LOCUS       DBNFCK010000001        78391 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.001, whole genome shotgun sequence.
ACCESSION   DBNFCK010000001 DBNFCK010000000
VERSION     DBNFCK010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 78391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78391
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.001"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            78..1667
                     /locus_tag="AC1PIW_000001"
     CDS             78..1667
                     /locus_tag="AC1PIW_000001"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HIP6604980.1"
                     /translation="MSKTIQAITNQIWSMANELRGNMDASEYKNYILAFMFYRYLSEH
                     QERFLVEDEIISPAPGETVQDAYAREAVGNDLVEHLENTASHLGYAIEPEDTWARLVA
                     KIDNSEIVASDYQTIFDHFNANVELNRDAMEDFRGVFNDINLGDSRLGNSTVVRAKSL
                     NSIVKLIDSIEYKNDEGKDILGEIYEYLIGQFAASAGKKGGEFYTPHQVSKILAKIVT
                     LGLEKSDTSFSVYDPTMGSGSLLLTVRNELPQGQHIKFYGQEMNTTTYNLARMNLMMH
                     QVGYSNMILNNADTLESDWPDGVDELGIDQPRSFDAVVANPPYSAKWDNRESKLKDPR
                     FMEYGKLAPASKADFAFILHSLYHLNNTGTMAIVLPHGVLFRGAAEGHIRKLIIEKNY
                     LDAVIGLPANLFYGTGIPTTILVFKKNRQTKDVFFIDASKEFEKGKNQNHLSDDMVEK
                     IVETYHNRQSVDKYAHLASIEEIVENDYNLNIPRYVDTFEEEEEIDLGQVTQQLEQDR
                     LEIRALEDKIRQQLKTLGVDF"
     gene            complement(1718..2992)
                     /gene="serS"
                     /locus_tag="AC1PIW_000002"
     CDS             complement(1718..2992)
                     /gene="serS"
                     /locus_tag="AC1PIW_000002"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIP6604981.1"
                     /translation="MLDIKRIRTDFDGVAAKLATRGVAAETLANIKELDAKRREILVT
                     VEELKAERNTVSAEIAQAKRNKENADDKIAAMQKLSADVKNLDAQLLEIDEELNAIIT
                     VLPNTPHDSVPVGADEEENVEVRRWGTPREFTFEPKAHWDLGEDLDILDWERGAKVTG
                     ARFLFYKNLGARLERALYNFMLDEHIAEGYQEIITPYMVNHDSMFGTGQYPKFKEDTF
                     ELADTNFVLIPTAEVPLTNYYRNEILEEKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFATPEQSYDELEKMTANAENILQKLGLPYRVLALCTGDMGFSAAKTY
                     DLEVWIPAQNAYREISSCSNTEDFQARRAQIRYRDAADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEVLRPYMGGVEVIKP"
     gene            complement(3210..3581)
                     /locus_tag="AC1PIW_000003"
     CDS             complement(3210..3581)
                     /locus_tag="AC1PIW_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HIP6604982.1"
                     /translation="MAQSQNTSVELQTTGVSYMGFGGKVGKFLIGDKALEFYPDSNVE
                     RYIQIPWSEMTSIGANVSGKAISRHFEIYTEKSRFLFASKDSGKILKIAREHIGNEKV
                     VKLPTLMQTIGRKISNLFAKK"
     gene            complement(3641..4552)
                     /locus_tag="AC1PIW_000004"
     CDS             complement(3641..4552)
                     /locus_tag="AC1PIW_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HIP6604983.1"
                     /translation="MSEKIQLSVSDRKKVWWRSTFLQGSWNYERMQNLGWAYAMIPAI
                     KKLYTKKEDQAAALERHLEFFNTHPYVASPILGVTLALEEERANGAEIDDTAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGALGASLALSGNIMGPIIFFLGWNIIRMAFLWYTQE
                     FGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVSITFAVDLPAKQLSEGA
                     YIVFPEGTVTGAELKGILGQALSGLSLEPTQAQTLQGQLDSLIPGLMGLLLTFLCMHL
                     LKKKVTPITMIIGLFIVGILARFFGIM"
     gene            complement(4570..5379)
                     /locus_tag="AC1PIW_000005"
     CDS             complement(4570..5379)
                     /locus_tag="AC1PIW_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HIP6604984.1"
                     /translation="MSDITLISAILVVIVAFFAGLEGILDEFQFHQPLVACTLIGLVT
                     GNLAAGVMLGGSLQMIALGWANIGAAVAPDAALASVAAAIIMIKGGDFSTEGIAVATT
                     TAIPLAVAGLFLTMIVRTISVGLVHGADNAAKDANFAAVERYHLFALLLQGLRIAVPA
                     ALLLAIPAEAVQAVLESMPAWLSGGMAVGGGMVVAVGYAMVINMMATREVWPFFALGF
                     AFAAISKLTLIALGVIGVAIALIYLNLSKQGGSGNGGASSNDPIGDILEDY"
     gene            complement(5411..6403)
                     /locus_tag="AC1PIW_000006"
     CDS             complement(5411..6403)
                     /locus_tag="AC1PIW_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIP6604985.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPSEGPDD
                     LYAKLNAAVDAFDADDEVLVLADLWSGSPFNQASRVAGENPDRKFAIITGLNLPMLIQ
                     AYTERMINAAAGVEEVAANIIKEAKDGIKVLPEELQPAEAAPVADAAPVALQGAIPPG
                     TVIGDGKLKINLARIDTRLLHGQVATGWTPASKADRIIVASDTVAKDELRKQLIKQAA
                     PGNVKANVVPIKKLIEVSKDPRFGNTHALILFETPQEALEAIEGGVEIKELNVGSMAH
                     STGKTMVNNVLSMDKDDVATFEKLRDLGVEFDVRKVPNDSKKDLFELINKANVQ"
     gene            complement(6625..7437)
                     /locus_tag="AC1PIW_000007"
     CDS             complement(6625..7437)
                     /locus_tag="AC1PIW_000007"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIP6604986.1"
                     /translation="MKKKIIAIDLDGTLLNNDSQLSDYTISTIKKVRQAGHTVLIATG
                     RPYRMAEKFYQQLELDTPMINFNGSLVHIPGKKWQWEQNILIDKKYLLEFLKEEETFE
                     ADFIAGEYKNKFYITQKNLDKIDPALMGVEQITPDTLIKPELITSDPHSILMQTRATD
                     KYELAKEMNAYFKDELEINTWGGPLNILETCAKGVNKATALSYVLNLYQASPSDLIAF
                     GDEHNDVEMLDLAGTAYAMKNCSDTLRPHADRLTAFANFEDGVARELENLFL"
     gene            7782..9242
                     /locus_tag="AC1PIW_000008"
     CDS             7782..9242
                     /locus_tag="AC1PIW_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HIP6604987.1"
                     /translation="MDQFFKLKEHGTTVSTEIMAGLTTFFAMSYILFVNPSVLSVAGM
                     PTQAVFLATIIASAVSTLVMGLFANVPYALAPGMGLNAFFTYTVVIGLGFTWQEALAM
                     VFLCGLFNVFITVTKVRKSIIKAIPVSLQHAIGGGIGVFVAYLGFKNSNLITFLTSGS
                     DIITVNGVAPADATAETFSNGVFSVFAGGGVVPGISTFTDPSVLLTVFGLLLTAVLVI
                     KNVRGAILIGIIATTVAGIPAGVVDLSTIDFANNNTGTAFAELGTTFLAAFDGLSSLF
                     ADSSRLPLVLMTIFAFSLSDTFDTLGTFIGTGRKTGIFSEEDEKALENGTGFNSKMDK
                     ALFADAIGTSIGALFGTSNTTTYVESAAGISAGGRTGLTAVTTAVLFLLSILILPFIG
                     IVPNAATAPALIIIGVMMVSSFLDVDWSHFEDALPAFFAAFFMALCFSISYGIAAAFI
                     FYCLVKVSTGKAKEIHPILWGATALFILNFIILAIL"
     gene            complement(9591..10451)
                     /gene="metF"
                     /locus_tag="AC1PIW_000009"
     CDS             complement(9591..10451)
                     /gene="metF"
                     /locus_tag="AC1PIW_000009"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HIP6604988.1"
                     /translation="MIGQTPSLSFEIFPPKPAVGNEKIIQTLDEMQGLAPHFISVTCS
                     NNNLNVEETTVKLANHVRNELHIPTIAHLPAAYLTKEKVRSVLHSLDEIGVHQILALR
                     GDIIEGLPPKEDFQYATDLISFIKEEAPHFDIIGACYPEVHPESPNSVSDIKNLKKKV
                     DAGCSTLVTQLFFDNEAFYNFQEKCSLADIEVPIIAGIMPIINKNQALRLLKTCENIR
                     LPRKFKAILEKYEHNPESLRAAGLAYAVDQIVDLVTNDVAGIHLYTMNNAETARTIHQ
                     ATHSLFKHYS"
     gene            complement(10455..12704)
                     /gene="metE"
                     /locus_tag="AC1PIW_000010"
     CDS             complement(10455..12704)
                     /gene="metE"
                     /locus_tag="AC1PIW_000010"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HIP6604989.1"
                     /translation="MTTTIIGFPRIGEHRELKFITEKYFRNEIPQEELLEAAKDLRAK
                     HWNIVKEAGITEIPSNDFSHYDNVLDAAVLFNIIPKAVQDLELTDLEKYFALARGYQG
                     EKGDVRARPMKKWFNTNYHYIVPAIEKDTEIKLAGHKIFDEFQEAKNLGITTRPVLIG
                     PFTLLQLTDFEEGLTATNFADSLVAAYGQVFEKLAELGAEKIQLDEPSLVKDLTAEEK
                     ALFLRIYQTLLADKKGLQVLIQTYFGDVRDIYTELTSLPVDAIGLDFVEGKETAALVA
                     TGFPADKTLYAGIVNGKNIWRNNYEKSLAVLDAIPAENVVITTSCSLLHVPFTTANEE
                     FEPAILNHFAFAVEKLSELRDLDAIRNGQGEVALTANKELFALERVGRDAALADRLAG
                     LTDTDYTRLPVFAERESIQREKLNLPLLPTTTIGSFPQTKEVRSTRLAFRKGNISEEE
                     YDAFVKTQTDEWIAWQEEVDFDVLVHGEFERNDMVEYFGENLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKE
                     STLQIALAIKEEVLDLEAAGIKIIQIDEAALREKLPLRRSDWYSEYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLVILDELKAKNFQTQVG
                     PGVYDIHSPRVPAVDEIAHTIQAILAKVPKEKVWINPDCGLKTRGESETKASLIHLTQ
                     AAKAARKEL"
     gene            13085..13774
                     /locus_tag="AC1PIW_000011"
     CDS             13085..13774
                     /locus_tag="AC1PIW_000011"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIP6604990.1"
                     /translation="MGKVIIGISGNEQEFPTKSGRVYVTVARELADSVRQAGGVPMVI
                     PMGTPDLAKDYIDMIDKLILSGGQHVDPSLYGQKRLIDSNDYLLERDEFELALIEEAL
                     HQGKPIFAVCRGMQLLNVALGGSLEQEVDHHWQDGIAGTSHRLQVKPKSRIGQLFAQG
                     SPINSFHQQRIKELAPGLVATGLDPRDGTIEAYESRGNQALFGIQWHPEFLYNDCKQH
                     RDLFQYLVDIF"
     gene            13924..14775
                     /locus_tag="AC1PIW_000012"
     CDS             13924..14775
                     /locus_tag="AC1PIW_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HIP6604991.1"
                     /translation="MKLKKLFSLATAALSVGVLAACGNSSSSSDSSATTVRVGVMSLS
                     DSEQARWDKVQEILGDEVKLEFTQFTDYSQPNKAVAENEVDINAFQHYNFLNNWNQEN
                     GEDLVAIADTYIAPIRLYSGTADGKNKYTKVEEIPDGAEIAVPNDPTNESRALYLLQA
                     AGLIKVGVSGTELATIADITENKKNLKITELDASQTASSLSSVDAAVVNNTFVLEAGL
                     DYKNALYKEQKDENSKQWYNLIAARSDWEKSEQAAAIKKIIEAYHTDEVKKVIEETSD
                     GMDEPVW"
     gene            14932..16299
                     /locus_tag="AC1PIW_000013"
     CDS             14932..16299
                     /locus_tag="AC1PIW_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIP6604992.1"
                     /translation="MADSKVQAFENDAIIQTYFEKLKVLISKKSIFAQQIGLLDVATY
                     LKDIFEEAGAEVVLDDSYAAPFVMARFEASVPDAKTLIFYNHYDTVPADADQVWEKGN
                     PFELTISDGYIYGRGVDDDKGHITARLSALKKYQARQNGHLPVNVIFIMEGAEESASV
                     DLDKYLSKYKEHLIGADLLVWEQGHRNSLHQLEIAGGNKGIVTFDLQVKSADLDIHSS
                     YGGVIDSASWYLLSALQSMRAADGQILVEGIYDQVQEPNERELALVEEFALATSQSMT
                     DIYGLTLPTLIEDRREFLKRLYFEPSITIQGLSTGYLGQGVKTIIPAQASAKMEVRLV
                     PGLEPHDVLDKIRQHLDKHGFDKVEVVFTLGEMSYRSDMSHPAIVNVIELAKKLTPEG
                     VAVLPTSPGTGPMHTVFHALGVPIAGFGLGNANSRDHAGDENVSIADYYSHVELVEEL
                     IASYE"
     gene            16292..17356
                     /locus_tag="AC1PIW_000014"
     CDS             16292..17356
                     /locus_tag="AC1PIW_000014"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HIP6604993.1"
                     /translation="MSKEMIKLDNIDVTFQQKKRTIEAVKDVTIHINQGDIYGIVGYS
                     GAGKSTLVRVINLLQVPTAGKITIDEDVIYEGEKVTLTPAQLRSKRREIGMIFQHFNL
                     MAQMTAEENVAFALKHSGLSKEEKKEKVAKLLDLVGLSDRAENYPAQLSGGQKQRVAI
                     ARALANDPKILISDESTSALDPKTTKQILALLQELNEKLGLTIVMITHEMQIVKDICN
                     RVAVMQDGRLIEEGSVLEIFSHPKEELTQDFIKTATGIDEALVKIYQQDIVKNLPENS
                     ILVQLKYAGSNTDTAIVNDLYKFYQVSANILYGNIEILDHTPVGEMVVILSGEPGQLH
                     RAIEAVTEARVEVTILKGAN"
     gene            17357..18049
                     /locus_tag="AC1PIW_000015"
     CDS             17357..18049
                     /locus_tag="AC1PIW_000015"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HIP6604994.1"
                     /translation="MLEWIQTNFPDIYKLGWDGQTGWLTHFNLTLYMTFVSFAFGGFM
                     GLVSGLFLVLTGPRGVIANKTAYWILDKVASIFRAIPFIILLAAIAPLTKIIVGKTIG
                     TEAALVPLALSVFPFFARQVEVVLSELDRGVIEAAQASGATFWDIVLVYLREGLPDLI
                     RVTTFALVSLVGYTAMAGAIGAGGLGQVALSYGYLRYNNDVTFLATLLILVIIFVIQF
                     IGDFLTRKISHR"
     gene            18231..18935
                     /locus_tag="AC1PIW_000016"
     CDS             18231..18935
                     /locus_tag="AC1PIW_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B3/B4 domain-containing protein"
                     /protein_id="HIP6604995.1"
                     /translation="MSQFVVDSSFWSLFPDAKIGVLLLKDYKTPAQSPDELVKLLEES
                     NGIAQKFLTEDTFSENEVIRTYRQAYQKFKTKKGARSSIEALLKRSTSDRPVTTISPL
                     VDIYNAASLRFGLPCGAEDLDTFVGNLQLTVTEGGDEFYLIGDETNNPTLPGEVCYKD
                     DKGAVCRCFNWRDGERTMITDETKNAFLVMELVNSDRVEDLENALDFISQHAEKFLGV
                     VPEKYLLDCDRPAIAL"
     gene            19286..19681
                     /locus_tag="AC1PIW_000017"
     CDS             19286..19681
                     /locus_tag="AC1PIW_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HIP6604996.1"
                     /translation="MFTLENINHGHEQFTGPDFPKLIAYFKDLGMVENIVDIQSGQVT
                     YRSQTGQTLEKQGYQVTIPVSDQANLDQFVTILRNHQAGQTDFLTFCQETAEAGIYKW
                     VIDLEAMTCSYMDKAEQAVFVETIPSVEN"
     gene            19992..20909
                     /gene="glyQ"
                     /locus_tag="AC1PIW_000018"
     CDS             19992..20909
                     /gene="glyQ"
                     /locus_tag="AC1PIW_000018"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HIP6604997.1"
                     /translation="MSKKLTFQEIILTLQQFWNEQGCLLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLER
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEVSDQDMLLENFT
                     KFEKEAERALEEGLVHPAFDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKKLGFPLLDEATRAELLKEDAE"
     gene            20909..22945
                     /gene="glyS"
                     /locus_tag="AC1PIW_000019"
     CDS             20909..22945
                     /gene="glyS"
                     /locus_tag="AC1PIW_000019"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HIP6604998.1"
                     /translation="MTKNLLVELGLEEIPAYIVTPAMHQLRDRMATFLTDNRLAFDSI
                     DVFSTPRRLAVRVRGLADQQTDLTEDFKGPAKKIALDADGNFTKAAEGFVRGKGLTTA
                     DIEFREIKGEEYVYVTKHEAGQPAKTVLAGIPEVLKAMTFPVSMNWASNKFAYIRPVH
                     TLTVLLDDEALDMDFLDITSGRISRGHRFLGNETEIASADSYEADLRAQFVITDPAER
                     QNMIVEQIKAIEDKHNVTVEIDEDLLNEVLNLVEYPTAFMGSFDTKYLEVPEEVLVTS
                     MKNHQRYFVVRDKAGKLLPNFISVRNGNDQYLDNVIKGNEKVLVARLEDGEFFWREDQ
                     KLKIADLVERLKVVTFHEKIGSLYEHMERTKVIAEKLADLAGLSAGEKADVARAADIY
                     KFDLLTGMVGEFDELQGIMGEKYALLAGEKPAVAAAIREHYLPNSAEGELPESKVGAV
                     LALADKFDTLLSFFSVGLIPSGSNDPYALRRATQGIVRILEAFGWEIPLDQLIAELYS
                     LNFASLTYDNQPAVMDFIRARVEKMMDKTIPKDIREAVLASSTFVVRLQLAASSAIFQ
                     KAKEADYKEAVENLSRVFNLAEKAEVTAIDEALFENDQEKALAAAVAGLELTEDMAGN
                     LDKLFALSPVIAAFFDNTMVMVDDATVKANRLALLKALADKASAVAVFNLLNSK"
     gene            22955..23212
                     /locus_tag="AC1PIW_000020"
     CDS             22955..23212
                     /locus_tag="AC1PIW_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HIP6604999.1"
                     /translation="MEQAKIDRINELARKKKAEGLTEEELQEQAALREEYIEGYRRSV
                     RAHIEGIKVVDEDGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            23667..24038
                     /locus_tag="AC1PIW_000021"
     CDS             23667..24038
                     /locus_tag="AC1PIW_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605000.1"
                     /translation="MKKLVYALLVLAYVTVAYLSVYVLPHYYSGAIIGAAGASLGASF
                     KQFKEVKASPDKALLAYFKRDEKKASSLLLVLLGIFLFVSLVLEFNWQYGLAFVVAIS
                     YAMLWNVLHIQFLRKYYFKNA"
     gene            complement(24060..25397)
                     /locus_tag="AC1PIW_000022"
     CDS             complement(24060..25397)
                     /locus_tag="AC1PIW_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIP6605001.1"
                     /translation="MNDLTKGKPIAVILQFAIPLLIGSFFQLAYNFADSMIVGHTLGK
                     DSFASVGSTASLIFLIIGFAQGVTNGLTIISAQRFGAGDLEGLKKSFVHGLFYASLIS
                     LLLTVSALAFLKPILVLMQTPVSIIDHSHAFLTAMFGGLTFTIFYNFLSSALRSLGNS
                     KTPLLALVIACFINIGLDFFFILVMNWGVFGAGFATILAQACSVLFLIFYIIHKVPHY
                     HIGLADLKLDRGNLKKHAQLAFPMGFQASIIAIGAMTLQFMVNQLGTDAIAAQAIALR
                     TDQLAMLPMVNLDLAIATFTAQNYGAKLYDRIREGVRHSLLLSIAWAIVFAVILILGN
                     RFFSGLFLPNASQTVLDLALVYYIINGSCYWIVASLFILRSFIQGLGKGFIPTLAGFG
                     ELIFRAAVAIIGMQYFGFYGTAAANPAAWIGSIIVLIPSSIIFMKKLKAGQSI"
     gene            complement(25718..26383)
                     /locus_tag="AC1PIW_000023"
     CDS             complement(25718..26383)
                     /locus_tag="AC1PIW_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIP6605002.1"
                     /translation="MPNYTIGILGLGVFGTTIAKTLHNYDCNIIAIDNHEQRINHLEP
                     ILTRGIVGDITDRSLLRAAGIGNCDAVVVATGENLESSVLAVMHSKVLGVPMVIVKVK
                     GTTAKEVLLRVGADKVISPERETGISLAKQLLHRDTTSLFELEGNVSIVEFHPPVKWI
                     GKTLGELKLRQHYKLNIIGYRSGENQELNIQLTPDYVFKSDELILAVTDHNTVDHFEE
                     LTN"
     gene            complement(26395..27744)
                     /locus_tag="AC1PIW_000024"
     CDS             complement(26395..27744)
                     /locus_tag="AC1PIW_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIP6605003.1"
                     /translation="MSPFRIKLSPSQRIIISFLFVILTGSILLSLPISQISTSTASYW
                     DHLFTSVSMVCVTGLFTKAVAETYSTFGQIICMMLIQIGGLSLIGFIGLFALRGGRKM
                     NFINMATLQEALNRSDTKYFRNFLKSAFGFTLAVEAFGALLLSFHFVPEFGWARGLFT
                     SIFVAISAFCNAGFDNFGATSIMRYVDNPLINITLASLIIMGGLGFSVWFDLQTQIGQ
                     KRSLRKLGFHTKIVLGLTAIILTLGTLTTLLTEWNNPSTIGQLSFGHKLLASFFQTVT
                     MRTAGFASIDYTKAEPITLLLYIFQMMLGGAPGGTAGGIKITAFLTLVLYARSEILGL
                     PHTNFMGRTIDNLSIRKAFATFSVFLLMFIIGLFALALTDDRQPLLFLMFEVMSALAT
                     VGVTANLTPSLTMAGQAVIMALMFFGRIGPLSILASLSVRKLSKTESLQYAKSSMLV"
     gene            complement(28008..28547)
                     /locus_tag="AC1PIW_000025"
     CDS             complement(28008..28547)
                     /locus_tag="AC1PIW_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HIP6605004.1"
                     /translation="MNSKRQTLNFITMLAAQAVVISLIERLITPPFAFAPGAKLGLGN
                     LISLIAIFTLPTKDSLKVVALRLLISTFLGGTFSTFLYGFAGTTLSYVGMLSAKQLGP
                     NRVSPIGISILGGMLHNLGQLLVFATIARSFYVLNYLPILAFTGILSGLLVGLAATYL
                     LQKVGPLRHYHQQVLAEWK"
     gene            28730..30622
                     /locus_tag="AC1PIW_000026"
     CDS             28730..30622
                     /locus_tag="AC1PIW_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIP6605005.1"
                     /translation="MTKNIVIVGAGYAGIAAARLLGKTFKKDQDVTVTLIDKNSFHTY
                     MTELHEVAAGRVEANAIKYDLQRIFKKYPKVQLVTDKVVEIDYDKKQVVAEHQTLDFD
                     YLLLAMGGEANDFGVKGVKEHGFTLWSIEAAERLHDHMIDACYRAMREHDEAKRRALL
                     TFTVIGAGFTGIEMIGELIDWVPILAREFKLDSKEFSLKVVEATPNILAMVTEKEQVK
                     ARKYLEKKGVELVLGDGVASVQEDSLTLSSGRQIPTYTSIWTAGVQANTDASEFGIEK
                     ARAGRLIANEFMEAKGKENVYVAGDLVYFEESEGKPTPQIVQAAEQTGHTAASNIIAA
                     IKGGEKHSYKGKYDGFMVSIGARYGVAFLMDKYHMSGFMAMAVKHMVNLLYFFTIRSF
                     FYMGSYVRHEFFDIQNKRNIFGGHTSGKGNLLWSVPMRVLYGSVWLYEGIKKAFGLFG
                     TTSWFGDQVVFPFPWLADPVSGASAAEAVSSASQAATAAAEVAEPIFGLSYAYGEAPM
                     AVLDKMPDWFATIMEFMMPNQEVALFMQKFMTVAEIGIGLALIAGAFVWIVSAATVAL
                     VVMFSLSGMFYWVNIWFIPAAISLMNGAGRAFGLDYWIMPWLGRFLDKKIYGKPKHIY
                     RIKDKK"
     gene            30641..31630
                     /locus_tag="AC1PIW_000027"
     CDS             30641..31630
                     /locus_tag="AC1PIW_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIP6605006.1"
                     /translation="MVHQIWNTYPEIERGLEEVKSIILSEMMVLHPAVTAKIVEYVEA
                     PGKYLRAGLCLLFAQMTEGKISRSKLYFAAHLEVLHLATLIHDDVIDGADKRRGVTAA
                     HQQFSNRIAIYTGDYLLAYSSRLFGKGLRLLEVSRDDLDLGNDKLMETILRGELSQLL
                     NQFDANMTMKAYLKQIQGKTAFLFGLACQLGSFVPGQSRKDSSMAFRSGQALGMAFQL
                     RDDLIDYQLDVAESGKPRLQDIQNGIYTAPLLFAMREDKSIQQQLRAYIQSPTAEGLE
                     ILTDRIFATHAIAKTEKLIVSYLEKMMSFVDRISQLSTASMQNLVAIVFSHYF"
     gene            31709..32188
                     /locus_tag="AC1PIW_000028"
     CDS             31709..32188
                     /locus_tag="AC1PIW_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HIP6605007.1"
                     /translation="MKTTKVVLKSLVVLGAVFALVACGSKQESTSTSSSTTETVVLKD
                     GTYKAESGKDDYGYKIVHTLTVKDGKISESKFDYEAEDGSLKSANEEYNKNMKDKAGV
                     SAGEAIEQLNAALVEKQDLSAVEVVSGATHTSEDFKKSTEALLAAAAEGKTETVKLD"
     gene            32266..33315
                     /locus_tag="AC1PIW_000029"
     CDS             32266..33315
                     /locus_tag="AC1PIW_000029"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIP6605008.1"
                     /translation="MKKIASLLFVGLLMVLLVACGGKQNESLSVVKTPLTRSESLLHT
                     VVQLSIYHENQEEAMDEAVSYIKEMESLLSTNLEGADVYRINQAAGKEAVKVDERTFE
                     VIETAIDMSKESQGLFDISIGAVSNLWKIGDEDARKPSDEEIKAALPFINYKDITLDK
                     EKKTVFIKEGMTLELGAISKGYIADKVRELFASKGITTAIINLGGNVVVMGDSPTTQN
                     GWNVGVQDPDQVRGSTVGSVPGTHDSVVTSGIYERYLEVDGVKYHHILDPKTGYPVEN
                     DISGVTVFSKTSIQGDAFSTTLFLLGVEKGLDFINQIDGVEAVFIDKEQGVHLSDGLK
                     ESFELTNEEYHLVNE"
     gene            33308..34264
                     /gene="menA"
                     /locus_tag="AC1PIW_000030"
     CDS             33308..34264
                     /gene="menA"
                     /locus_tag="AC1PIW_000030"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HIP6605009.1"
                     /translation="MNKSTKGLSLSVFLEFVEMKTKVASVFPMTLGILWSLYRYQAFN
                     WLNTLLFIIAVLSFDMCTTAINNSMDYHKAKDEAYRQESNVIGKFSLDFRQMIGIVLA
                     LLIFSLLVSLLLVWRTSWLLLPMGALCFLIGIFYTFGPIPLSRMPLGEVFSGVTMGFG
                     IFFLAVFIQQPDLLLTSQVSGQWMTLQFSWIKIIEIIFMSIPLVTLIANIMLANNTCD
                     LEEDIRNHRYTLVYYIGKKNALKLYFVLASLPWLLWIIYCLIGFLPIWALAGLIGVWP
                     AYKSLETFLKKQLKRETFIEAVKSFVLFALLYVVILALALIF"
     gene            complement(34572..35000)
                     /locus_tag="AC1PIW_000031"
     CDS             complement(34572..35000)
                     /locus_tag="AC1PIW_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HIP6605010.1"
                     /translation="MTFKSILKQLKLFDYILIGFTLVLSFLPAIFTYTQLTTDANEAK
                     TIAYVRINGEVVDQFELSKDTPRQEKTYYPNEGQYNIIEVDGERIRVKEDNSPDQIAV
                     MTSWISQPGQLSVCLPHNLLIEIKSVGGENTDEEELILPL"
     gene            complement(35053..35400)
                     /locus_tag="AC1PIW_000032"
     CDS             complement(35053..35400)
                     /locus_tag="AC1PIW_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605011.1"
                     /translation="MNVNKPKLILTLIVLSYTTFCSLYIHSYLTGDNPPIFILPFTIL
                     PILNSDWQTTSPESNAVQEAIHLYRKPLLCFLFAGLVLTFSIGFFTGQIKPLLAGFWG
                     IFALLLFIGQLKK"
     gene            complement(35390..35734)
                     /locus_tag="AC1PIW_000033"
     CDS             complement(35390..35734)
                     /locus_tag="AC1PIW_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIP6605012.1"
                     /translation="MNKPFTPKQTCRFFVLFYALFSAFFIYSYMVNEPISGSFLLMLP
                     PIQIIRSFKTEEEKRYFIIQAIFALFAMTLTYSVGIWLGHVKPHILILPAGFALAILY
                     RLYFKKEKTDER"
     gene            complement(35747..36829)
                     /locus_tag="AC1PIW_000034"
     CDS             complement(35747..36829)
                     /locus_tag="AC1PIW_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HIP6605013.1"
                     /translation="MKKETFQDKLIKRFYGIAGPLDEFRQKEASRLGNTCFILLFWGT
                     MALTLLALALSKRYPEVVAYGYPTALLLSTLSSSMYMTSKMRHSQVDSLDVEELTTKE
                     QKQLKGASIKFALYFTAVMYIWTVVFGAWMEGLNPFDHLFDLRKFLAACLGGIFTGIA
                     LEIMLRKRMKKAENLTISSAISKEEPKWIKNMIKRFYGIRGPLDEYRQAEADAIGGQA
                     FIYYFYFLALGNAIAYFLAYRYPVEVAAYYPMIIAFFSIILIGIVNMRTLHADLPQYD
                     MEELSPEERQGQTLNPLVWGLGVALLSSLFAGLSDLFSLQLPLLASSFHVKSLLFGGM
                     MGLFASLALSALAYLQKLEADTAKKK"
     gene            complement(36840..37040)
                     /locus_tag="AC1PIW_000035"
     CDS             complement(36840..37040)
                     /locus_tag="AC1PIW_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605014.1"
                     /translation="MKKNIRQALISTLIFIPLFVLFQLWGNSGAEILHTISQAKFWLQ
                     LLIAGTIYFLGFVYILPLFVQK"
     gene            complement(37055..37588)
                     /locus_tag="AC1PIW_000036"
     CDS             complement(37055..37588)
                     /locus_tag="AC1PIW_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HIP6605015.1"
                     /translation="MKDIFTKILNQATKDFYSISGPMDEMRLQESYRLSNTIVMIVFP
                     ILLIGNTIALLIALRQPEKVGFLLPLTNILIIGFTQALASHLALKNGISQSYEDEINV
                     TKLNKSLVKSSRSTFFGAFLASTTAPAFYKEWSVFLEELTDPTLLLGRLIVAGAITFI
                     HYYYCKKQLQKKILANK"
     gene            complement(37599..37787)
                     /locus_tag="AC1PIW_000037"
     CDS             complement(37599..37787)
                     /locus_tag="AC1PIW_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIP6605016.1"
                     /translation="MNRVKDYRLLLGISQLDLAKAIGVSRQTINMIENNKYNPSLDLC
                     INLAKALQTDLNSLFWNQ"
     gene            38058..39008
                     /gene="rsmH"
                     /locus_tag="AC1PIW_000038"
     CDS             38058..39008
                     /gene="rsmH"
                     /locus_tag="AC1PIW_000038"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIP6605017.1"
                     /translation="MTKEFNHTTVLLHETVDMLDIKPNGIYVDATLGGAGHSEYLLSQ
                     LTDGGHLYAFDQDQTAIDHAHIRLASYIEKGQVTFIRDNFRNLKTRLAELGVTEIDGI
                     CYDLGVSSPQLDERERGFSYKQDAPLDMRMNREGHLTAYDVVNNYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQARAVKPIETTTELAELIKSAKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAIDLLALDGRISVITFHSLEDRLTKQLFKEASTIDVPKGLPFIPD
                     DLKAPLELVNRKPILPSQEELEANNRAHSAKLRVAKKVHK"
     gene            39019..39342
                     /gene="ftsL"
                     /locus_tag="AC1PIW_000039"
     CDS             39019..39342
                     /gene="ftsL"
                     /locus_tag="AC1PIW_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HIP6605018.1"
                     /translation="MLQEKRREATMRVIGDKIRTFTRVEKAFYSSIVLSGLALAIGII
                     FMQTRLLQLQSSMAKVNQDISQKQIEINDAKQAVNELTRSARLMEIAEKAGLTFNNDN
                     IGVAE"
     gene            39342..41591
                     /gene="pbp2X"
                     /locus_tag="AC1PIW_000040"
     CDS             39342..41591
                     /gene="pbp2X"
                     /locus_tag="AC1PIW_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP2X"
                     /protein_id="HIP6605019.1"
                     /translation="MPRRNNKLLRYVLKKRYVPSKNRRRVGQSLLLLTVFVFFLFLIN
                     FAYIIGTDSKFGVDLSERADAVHQREVIVQAKRGTIYDRTGIPIAEDSTTYTVYAIID
                     TEYVSELKKVLYVQSSQFSKVAEIFKKHLGMETDYVMEQLNQPELKQVYFGTLGKNIS
                     YSTMSSIQEEMKAAGIEGIAFNASPGRMYPNGNFASIFVGLANLVENKDGSHSLQGVS
                     GLEYYLNDILAGQDGKVIYEKDSQGRVLPGTEKVETETVNGQDVYTTISADLQRSLET
                     NMNTFFDNAKGRYANATLVSAKTGEILATTQRPSYDSDTKEGLGQEGLLERSLLYQEA
                     FEPGSTMKVLTVASAIDNGTFDPNAYYTNNEYVIADAKINDWTLNAGYSAVTLNYAQG
                     FSLSSNVGMTLLQQQMGDEKWLNYLTKFRFGYPTRFGMGDEAPGMVPEDNIVSIAMSS
                     FGQGISANQAQMLRSFTAIANNGIMLEPKFISALYDPNTDTARVSSKEEVGNPVSEQA
                     ADDTLRYMINVGTDPVYGTLYSHDLQGPAIQVDGYDIAVKSGTAEIASEDGQGYIKGA
                     YINSVVAMIPAEDPEFIMYVTIRQPEVLNVLSWRDIVSPTLKEAMLLKDNLNLDSPAP
                     ALARVANETEYQLPDLIGKAPGESAEELRRQLVQPVILGNGAEIKKVSVEKGSKVIAN
                     QQVLLLTNKFEEMPDLYAITKENMDIFAEWTGIEVTYKGTGSKVVDQSVEVGTALNKT
                     KKITITLGD"
     gene            41593..42591
                     /gene="mraY"
                     /locus_tag="AC1PIW_000041"
     CDS             41593..42591
                     /gene="mraY"
                     /locus_tag="AC1PIW_000041"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIP6605020.1"
                     /translation="MQFALMSGLVAFLATVLLIPRFITFYQAKRIEGQQMHEDVKQHQ
                     FKAGTPTMGGTVFLVVAILVSLLFATAFQLLTGGVLAILFILALYGVVGFLDDFLKIF
                     RKINEGLNPKQKLALQILGGIVFYFVHVRGAGGGELNVFGHMVHLGVLYFPFVLFWLV
                     GFSNAVNLTDGIDGLASISVVISLLAYSVIAFNEQKFDILLVCVTMIGGLLGFFVYNR
                     KPAKIFMGDVGSLALGGMLATISIALRQEWTLLLIGLVYVIETSSVMLQVSYFKYTKK
                     RFGEGRRIFRMTPFHHHLELGGLTGKAEKWSEWKVDFFLWSVGLIMSLITLAILYL"
     gene            42684..43517
                     /locus_tag="AC1PIW_000042"
     CDS             42684..43517
                     /locus_tag="AC1PIW_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605021.1"
                     /translation="MEQFNQEQLYGVVIQTMTAASQLPMVKVNREEFLKTRFKDSPYL
                     DKIIADGPTAVFTTKALRKEAEKIINDMTTKTSLISFAAGLPGNPFTAVAAGTADIAQ
                     YFGFALNLSQQIAYLFGEDELFVGEGKEISEEVQIRIIAYIGVMFGAGGSAVLLNKVS
                     KTVGANIGKKVAMQALTKTTWYPLVKKVAAVIGVKITKKTVEKTIANAVPILGGLISG
                     GLTYFTFQPMGGMLADTFVKKINGDFDGELELTEAFKASLEEEKELGIIEAEFTEASE
                     S"
     gene            43596..44939
                     /locus_tag="AC1PIW_000043"
     CDS             43596..44939
                     /locus_tag="AC1PIW_000043"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIP6605022.1"
                     /translation="MKFTDMKLKPYIQEALKEINFVQPTEVQEKLIPVVLSGRDLIGE
                     SKTGSGKTHTFLIPIFQKLNEELDQVQAVITAPSRELATQIYQAARQLASHSDTEVRV
                     TNYVGGTDKNRQIDKLQASQPHIVVGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEQIKTHTVISDTIE
                     NWLISTKGRDRNAQILEVTKLMQPYLAMIFVNTKTRADELHSYLTANGLRVAKIHGDI
                     PPRERKRMMNQVKNLDYQYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLSGIAITLYQPSDDADIRELEKLNIKFLPKEMKNGEFVDTYDRDRRANREKSREK
                     LDLEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRVEARAKGRAERKAKRQTF"
     gene            44957..45181
                     /locus_tag="AC1PIW_000044"
     CDS             44957..45181
                     /locus_tag="AC1PIW_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HIP6605023.1"
                     /translation="MLQNILSFYLQGLLIASLLIITSSLVWFIWRATKGVDKTLQERQ
                     DFLFDLLMINVMTIPIAAFGVVGILLMFKA"
     gene            45195..46112
                     /gene="trxB"
                     /locus_tag="AC1PIW_000045"
     CDS             45195..46112
                     /gene="trxB"
                     /locus_tag="AC1PIW_000045"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HIP6605024.1"
                     /translation="MYDTVVIGAGPAGMTAALYAGRSNLKVALLERGIYGGQMNNTAE
                     IENYPGYDHISGPALAEKMFEPLEKFGVDHIFGTLVRIEEEGQIKKVITEDGVLETKT
                     VVLAMGAKHRLLGIPGEDTYNSRGVSYCAVCDGAFFRGQKLLVVGGGDSAVEEALFLT
                     QFAESVTIVHRRDQLRAQKVIQDRAFANEKINFIWDSVVEEIKGDDLRVQSVVIKNVK
                     TEEVSELDFGGVFIYVGLDPMTDTVADLGITDEAGWVITNEKMETTQSGIYAIGDIRQ
                     NQLRQIATAVGNGAVAGQEVYNYITELAE"
     gene            46122..46661
                     /locus_tag="AC1PIW_000046"
     CDS             46122..46661
                     /locus_tag="AC1PIW_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIP6605025.1"
                     /translation="MFHIYDIQKNLDGIFFDKTLDLQEELQARNGEVLGLSPVQVTGN
                     VRFESGFFFLDYQMTYDITLASSRSLQPVVLHEVQNVNELFVANEAVLKEQDLIDEDM
                     VLVVEDDHIVLDESVADNILLAIPIKVLTPEEEAGQELPSGKAWALMTEEDFQQKAQE
                     KKEANSPFAQLQGLFGDED"
     gene            46758..48185
                     /gene="gndA"
                     /locus_tag="AC1PIW_000047"
     CDS             46758..48185
                     /gene="gndA"
                     /locus_tag="AC1PIW_000047"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HIP6605026.1"
                     /translation="MTKANFGVVGMAVMGRNLALNVESRGYSVAIYNRSADKTEDVVA
                     SNPGKNLVPSYDVESFVASIEKPRRIMLMVQAGPGTDATIQALLPHLDEGDILIDGGN
                     TFYEDTIRRSKELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGAPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSVDEMAD
                     IFTDWNQGELDSYLIEITADILKRKDDQGQDGPIVNYIMDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKDERVAASKVLPKPAPFAYEGDKAELVEKIRQALYFSKI
                     MSYAQGFAQLRVASKENNWNLPFGEIAKIWRAGCIIRARFLQKITDAYGRDEDLANLL
                     LDEYFLDVTAKYQQAVRDVVALAVQAGVPVPTFSAAITYFDSYRAENLPANLIQAQRD
                     YFGAHTYNRKDKEGIFHYDWYSESK"
     gene            48267..48956
                     /locus_tag="AC1PIW_000048"
     CDS             48267..48956
                     /locus_tag="AC1PIW_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIP6605027.1"
                     /translation="MAKKILIAGKERNLSHFVSMELQKKDYLVDYASTGKEAMSLAHE
                     TDFDLILMSFQLSDMSSKELATELLAIKPATVMIVVVEPTEVSQYGEEVLSYAVSYVV
                     KPFVISDLVEQISAIFRGRDFIDNNCKQVHMHAAYRDLKVDFQNRTVTRGDELINLTR
                     REYDLLATLMNSPEPVSREQLLERVWKYEAASETNVVDVYIRYLRGKLDLPHQDSYIK
                     TVRGVGYAMRD"
     gene            49046..50176
                     /locus_tag="AC1PIW_000049"
     CDS             49046..50176
                     /locus_tag="AC1PIW_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIP6605028.1"
                     /translation="MNTSFKEKVILILLSGATLLAVLNWSSIYGAIQQLLGSMNSLFI
                     GAIFAFILNVPMKKLEDQFEKISFLKKSKRSLAIVGVLIGFALIVTGLVVIVLPTLVT
                     TVSELVSVSSTAIPKSVNALTNFLESNGLLSGQIGDQIASFLDQLKNLTLISSLVTPI
                     LSGLVSNVTGIFSNTMTLIMAFFFTLAILGSKEHLQAMTRKFLQATLPEKAVRVVNYI
                     GEVIVDTYDRFLMSQIVEACIIGTLVFVSYSLSGIPYASMAGILAGVLSFVPYIGPFS
                     ACLISALFVAVQNPLLALWSIALFQIIQLIEGNVIYPRVVGQSVGLPTLFTLAAALIG
                     GNLFGLLGMVFFTPIFAVVYRLVREWVASRLKKQDELLVESE"
     gene            50248..50739
                     /gene="nrdR"
                     /locus_tag="AC1PIW_000050"
     CDS             50248..50739
                     /gene="nrdR"
                     /locus_tag="AC1PIW_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HIP6605029.1"
                     /translation="MRCPKCQSLKSSVIDSRQAEDGNTIRRRRSCDQCGQRFTTYERI
                     EEKTLVVVKKDGTREQFSREKIFNGIIRSAQKRPVSTDDIDEVVNRIEQKVRAQGDSE
                     IESDVIGNLVMEELVELDEITYVRFASVYRSFKDVGELENLLKQMISKGSKVKPGKKD
                     ETK"
     gene            50726..51877
                     /locus_tag="AC1PIW_000051"
     CDS             50726..51877
                     /locus_tag="AC1PIW_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation/membrane attachment
                     protein"
                     /protein_id="HIP6605030.1"
                     /translation="MKPNDLFAYIKISPFTPDIVSLSKCYQPIIGFDALALYYYFYSF
                     ADQGQGRYKWTVILNHMDFGMNRLEKALDVLTAMGLLELYRADELTGLALQAPLTVKS
                     FLAKPLYKNLLARKIGESSMENLLVHQPNQEQNISKTFSQVFTMDGQAPVVFEPKHDF
                     DWSAFKALMAKDKLLFQDEAEDIIALSYIAEQAGWTWLETYRQAKATAIGQMISTKRL
                     QQSRQSLPTESANLTAQEQAIVREAKSKSSLTFLSFIKEQRKAAVTGTERKCLTDLAN
                     LGLLDEVINVLVLYTFNKVDSANLNEKYAMKLGNDFSYKGIGSAEAAVLYLRELKTGQ
                     AVTKSSQPQKSNVPEWSKEEVVMDNSPEAQARMEALRKRMLENESKGGS"
     gene            51877..52776
                     /gene="dnaI"
                     /locus_tag="AC1PIW_000052"
     CDS             51877..52776
                     /gene="dnaI"
                     /locus_tag="AC1PIW_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HIP6605031.1"
                     /translation="MKSVQDRLSQATNPSSKSYQQLYQEIVSDPEVAAFIKKEGLTQQ
                     EITLSISKFLEYISQRDLFVKQDETYIAKGYQPVLVMNEGYADVSYLETEELVEYRRL
                     EAIKNRIQLINMPQALKKVTIADLDFTDSNRIEVYDAIDAFVEQVGEHYKGLYLYGDF
                     GIGKSFLMAYMAHRLSTNRHLSTTILHYPTFVVDIKNAIKDGSVKERIDEIKLAQVLV
                     LDDIGAEQHSPWVRDDVLQVILQYRMQENLPTFFTSNFSFDELERHFASGKSGDETWQ
                     AQRVMERIRYLARDLHLKGNNRR"
     gene            52773..53485
                     /locus_tag="AC1PIW_000053"
                     /pseudo
     CDS             52773..53485
                     /locus_tag="AC1PIW_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647269.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
     gene            53504..54814
                     /gene="der"
                     /locus_tag="AC1PIW_000054"
     CDS             53504..54814
                     /gene="der"
                     /locus_tag="AC1PIW_000054"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIP6605032.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRKFSLIDTGGIDDVDAPFMEQIKHQAEIAMDEADVIVFVVSGKEGVTDADEY
                     VSRILYKTNKPVILVVNKVDNPEMRNDIYDFYSLGLGDPYPVSSVHGIGTGDVLDAII
                     ENLPAQEAEENPDIIKFSLIGRPNVGKSSLINAILGEERVIASPVAGTTRDAIDTHFT
                     DPEGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIERSDVILMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKQWEDDIRDQFQYLSYAPIIFVSALTKQ
                     RLHKLPEMIKAISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVVFVNEEELMHFSYMRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            54882..55220
                     /locus_tag="AC1PIW_000055"
     CDS             54882..55220
                     /locus_tag="AC1PIW_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIP6605033.1"
                     /translation="MVAVPIWRSFLILLSFSSFFLVFYEDSQYKPFGFRYWLGLVACI
                     WLNLVTLASYFIAFTGGSIMVYNQYKQPLVVIFLFCLFVASMLTLLSFKAIKMMIRRT
                     KYYRQVRKVG"
     gene            55222..55530
                     /locus_tag="AC1PIW_000056"
     CDS             55222..55530
                     /locus_tag="AC1PIW_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605034.1"
                     /translation="MEFLGELLVEFLTGLADFDEKKHPPFGIRYWLGWLGVLIHVLLL
                     ALLTCVTVFFFKFFLVGKGLINVVVAVVFLLFALFWLWKFGKTILKMWQATIYYLAIH
                     "
     gene            55669..58761
                     /locus_tag="AC1PIW_000057"
     CDS             55669..58761
                     /locus_tag="AC1PIW_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HIP6605035.1"
                     /translation="MGRMMPGRIRQEGIDLYEAGKLTVLHSENGKMVLDIAGERFTYG
                     DADSDLQCSCQLFQSKGYCQHLAATEYFLKNDASGKDMKQSLKEDGEQHKETVRRTYF
                     GGLFLDEILRPEPELGIKYQLSVEGSLLPYDRQIDWTLKITRLPDTRSYIVRDIGAFL
                     RIVKANGHYQIGKNYYEQVTYENFDEPSQALLDFLWALVPEKVGIDSDILTHFGRHFR
                     LPQAYFEEGLELLNQLEHFNFSYQQQSYSSLMVLPLTGEEGLYHFEVTVHTQMIEMVI
                     HEKTVRPLFHGRYLLVNGTVYSVNRHQEHLIYQISELVPTESGLRKVQVDFPDQNRLA
                     LSLLDLQTIGTVKAPKRFIIHDFKPEFHIQMEANDSLSLQLVLDFDGRKVRSEEDLAL
                     LPFASHFQHLERVYQAIRLAGFRGTYHAQRAALSQQELYPFFQQQLPILQKMGQVHLA
                     ERLQALYIEAKPQLEIVRNGSLLDISFDLSGIEQSEIDQAITALLNQEDHYTSPSGKV
                     FVFDEETKKISQTLIYLRARHGKEGQVQVHALAGYQLAQSLSQFNQVSFSKEFEEMAS
                     YLAQPDLFPLPAVEVTTSLRDYQQTGLKWLTMLDTYGFGGILADDMGLGKTLQTIAFL
                     SSRMTKDSKVLILAPSSLIYNWLDECKRFAPSLDVAVVHGNKEQREEIIANDHQVLVT
                     SYPSFRQDVALYRQERFDYLILDEAQVMKNAQSKIAQLLREFEVGNCFALSGTPIENH
                     LTELWSIFQIVLPGLLPGKQDFGKLAAKDIARTIQPFVLRRHKEDVLQELPDLIEVNV
                     LNELTDEQKAIYLAQLQQMRTQIAGADDAQINRSKIEILSGITRLRQICDTPSLFMEE
                     FSGESGKLNSLKELLLQLKEGEHRVLIFSQFRNMLEKIEEQLIEIGMTSYTLTGSTPA
                     NQRQEMTQAFNAGSRDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAISRAHR
                     MGQTEKVECYRLITRGTIEEKIQELQENKKNLVKTVLDGNESRANLTVEDIREILGIE
                     "
     gene            58840..59502
                     /locus_tag="AC1PIW_000058"
     CDS             58840..59502
                     /locus_tag="AC1PIW_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605036.1"
                     /translation="MIRHEKRFPLVADDEVLVGENPIMSLYDESDLISNIRGPYQEKE
                     FSWSTDSQRVASAKPVAQTEDELLPPLFEAKPSHYSRKERLQQLTKTKPSPVKTQGQL
                     AREQAREDLKKKRSATYLRDEKPAPAKVVVKPPVVSTAESKAGRLTRLADNLRQTDYI
                     LADMPAVYSLKKEDREQEQVIKKNSYDFLRKSQVYNYPERRQQRDRQVAQELNLTHIE
                     EE"
     gene            59504..60835
                     /gene="murC"
                     /locus_tag="AC1PIW_000059"
     CDS             59504..60835
                     /gene="murC"
                     /locus_tag="AC1PIW_000059"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIP6605037.1"
                     /translation="MTKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGIQILPFDEKNITADVELIAGNAFRPDNNVEIAYADAQGYTYKRYHEFLGEFMKG
                     FTSLGVAGAHGKTSTTGLLAHVMRNITDTSFLIGDGTGRGSANAQYFVFESDEYERHF
                     APYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQVKNALFVFGEDEQLRRITANA
                     PIYYYGLEDNNDFVAYDLKPSTSGSQFKVRHGEEELGKFQIPTFGKHNVMNATAVIAN
                     LYIAGFDLQLVAEHLKTFGGVKRRFTEKIVNDTVIIDDFAHHPTEIIATIDAARQKYP
                     SKELVAIFQPHTFTRTIALLDEFADALNGADAVYLAQIYGSARETDNGQVKVEDLAAK
                     INKKGGLVTVENTSPLLDHDNAVYVFMGAGDIQSYEYSFERLLSNLTNNVQ"
     gene            60891..61376
                     /locus_tag="AC1PIW_000060"
     CDS             60891..61376
                     /locus_tag="AC1PIW_000060"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6605038.1"
                     /translation="MEIRFATPSDLEQVVLIENANFSKEERIAESVLATYLNTLSKTC
                     LIMEHDEEIAGYLLSCPSVSQTVTDDIFYLTESDMPTGGHLAIASLSVADAYKGQGVG
                     TLLLAAIKEVALAGGFEGVSLTCKRYLISYYEMNQFEDFGPSLSQFGGQRWSDMYWKA
                     L"
     gene            61448..63265
                     /gene="mltG"
                     /locus_tag="AC1PIW_000061"
     CDS             61448..63265
                     /gene="mltG"
                     /locus_tag="AC1PIW_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIP6605039.1"
                     /translation="MTKDTNEKNTQSSGFRDQILRDLEELKVKRLAEQSADVLVDKRN
                     EVINELQPSLAEEKAPHRETEIVKVEGIAFPDSYLSEEQQESEVMTESIEEPTSETLA
                     TPETFVIEKEVVSSPVPQDTVERNLEKLRNLVAANTAGFDEVPSSSSVSEPVESVLSE
                     KSSLEDTFLEFPTEDVSAVTGDTEIIALEETIIAPKTTAIVPEVDKQMQETQPRRRTT
                     HRTSKQRRKKQDNAAKHIVSVIMSIVVVAVLVTGVTGYMWVKSSLEPVNAKATETIQV
                     EIPEGSSTLEIGKILVDNKLIKNATIFNYYSKIKSYNNFQSGFYNLKQNMSVDDIAKA
                     LQESGTPTAQKEAAGKVLIVEGYTLTQIAQAITDNTNTKDKNDKTPFTAEQFMATVTN
                     QDFINRMVATYPKLFASLPAADSGVIYQLEGYLFPAVYEYSDETTIEELVEQMIAAMD
                     NRLQPYYETITAKNLTVNEVLTLASLVEKEGSTDEDRRNIASVFFNRLNAAMPLQSNI
                     AILYAQGKLGQETTLAEDAAIDTSIESPYNIYWTPGLMPGPVDSPSLSAIEAVINANT
                     TDYLYFVADVTTGNVYFTNNIDEHNQNVAKYVNAHLNNE"
     gene            63339..63821
                     /gene="greA"
                     /locus_tag="AC1PIW_000062"
     CDS             63339..63821
                     /gene="greA"
                     /locus_tag="AC1PIW_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIP6605040.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEIVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISTIETKIRYAEIVNSDAVAADEVAIGKTVTVQEVGETE
                     EEVYHIVGAAGADVFANKISNESPIGHALIGKKTGDVATIETPAGSYDVKILKVEKTK
                     "
     gene            complement(64020..64934)
                     /gene="yidC"
                     /locus_tag="AC1PIW_000063"
     CDS             complement(64020..64934)
                     /gene="yidC"
                     /locus_tag="AC1PIW_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HIP6605041.1"
                     /translation="MKNKRLLILSGMALSTLLFLSGCVQTDKSGNPTGPIWDWLGQPM
                     SNLIRFFAEDQALGFGLAIIIVTLLVRFIILPLGIYQSWKAAIQSEKMNYLKPILGPI
                     QERMKNASSQEEQLAAQQEYFATQKQYGVSMLGGIGCLPMLIQMPFFSAIYFAARHTP
                     GISEATFLGINLGSTSLILTVVAGILYYAQSLLMQVGMDEEQKKQMKTMALMNPIMIM
                     MFSFGSPAGVTLYWVVGGLFGILQQAITNFILKPRIRKQVEEEFKNITVSATPRKTKD
                     VTPTASAVIEEKTSNKKRNRNAGKQRSR"
     gene            complement(65004..65285)
                     /locus_tag="AC1PIW_000064"
     CDS             complement(65004..65285)
                     /locus_tag="AC1PIW_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HIP6605042.1"
                     /translation="MRKVKMIASGRVQGVGFRWSVQFLAVEIGDIYGRVWNNDDGTVT
                     ILAQSDNAEKLSHFIHEIRKGPSRMAKVIYLDVTLANFEDYKDFQVSYR"
     gene            65415..66155
                     /locus_tag="AC1PIW_000065"
     CDS             65415..66155
                     /locus_tag="AC1PIW_000065"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIP6605043.1"
                     /translation="MEIIRSKANHLVKQVKKLQQKKYRTSSYLIEGWHLLEEAMEAGA
                     NIEHIFVVEEYFEKVAGLANVTVVSPEIMQELADSKTPQGVVAQLALPSQRLPETLDG
                     KFLVLEDVQDPGNVGTMIRTADAAGFDGVFLSDKSADIYNMKVLRSMQGSHFHLPVYR
                     MPISSILTALKSNQIQILATTLSSQSVDYKEITPHSSFALVMGNEGQGISDLVADEAD
                     QLVHITMPGQAESLNVAIAAGILLFSFI"
     gene            66202..66684
                     /locus_tag="AC1PIW_000066"
     CDS             66202..66684
                     /locus_tag="AC1PIW_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIP6605044.1"
                     /translation="MVAYKQDKEYMHYVGHLIATPKVQKLGKIPHHYHSTRLEHSINV
                     SYTSYKIAKKFGWDAKSTARGGLLHDLFFYDWRDTKFNKSHAWVHPRIAKRNAQKLIQ
                     LNKLEEDIIVKHMFGATIAPPCYKESWIVTCVDKYWAVREWSLPLQHKWKNRKVFRFQ
                     "
     gene            66706..67407
                     /locus_tag="AC1PIW_000067"
     CDS             66706..67407
                     /locus_tag="AC1PIW_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HIP6605045.1"
                     /translation="MNNDSFIINQVDNTALNRFFGKIYGVVAMGIGLSALVSFLAVTV
                     FQSLLLSLLSAGSIIMMLIMIGQIALVVSASAMAAKNSPMALPMFLAYSVTNGITISM
                     ILMFYTSETVVLAFVSAALMFAIMAVIGMTTKKDLSGMAQALRAALWGIIIASVINIF
                     LRSSGLSFMMSIISVLVFSGLIAYDNQRIRNVFEQTGGNVGQGWVVSMALQLYLDFIN
                     LFLNLLRIFGGLSRD"
     gene            complement(67456..68787)
                     /gene="pepC"
                     /locus_tag="AC1PIW_000068"
     CDS             complement(67456..68787)
                     /gene="pepC"
                     /locus_tag="AC1PIW_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HIP6605046.1"
                     /translation="MSTLDFDFTERLYANYLANPSLQATENAVSHNGLLKSLETRQSA
                     IDNDYVFSIDLTKDAVSNQKASGRCWMFAALNTFRHKLISDFKLENFELSQAHTFFWD
                     KYEKSNWFLEQIIATADQEIGSRKVKFLLDTPQQDGGQWDMVVALFEKYGVVPKSVYP
                     ESISSSASRELNQYLNKLLRQDAQILRDLLAKGASPEEVQTQKENLLQEIFNFLAVNL
                     GLPPRSFDFAYRDKDNVYHRDTNVTPQAFYEKYVGLKLSDYVSIINAPTTDKPYNKSY
                     TVELLGNVVGAPAVRYLNVEMNRFKELAIAQLKAGESVWFGSDVGQSSNRQTGIMATN
                     TYDFSSGLGIHFHQDKAGRLDYSESLMTHAMVLTGVDLDDNEQPLKWKVENSWGDKVG
                     DKGYFVASDSWMDEYTYQIVVRKEFLTQEELAAYQAQPQVLAPWDPMGALA"
     gene            68921..70168
                     /locus_tag="AC1PIW_000069"
     CDS             68921..70168
                     /locus_tag="AC1PIW_000069"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HIP6605047.1"
                     /translation="MTKTPFVSKEVLENITEQFPTPFHLYDEKGIREKARALNAAFSW
                     NKGFKEYFAVKATPTPAILKILQEEGCGVDCATDVEVLMSEKLGFKDIMFTSNDTQAQ
                     EFVYARKVGATINLDAYEHIEFLKNVAGIPETVCLRYNPGGVFSLGTDIMDHPEESKF
                     GMTKDQLMKGYKELKELGVKQFGIHAFLASNTVTNDYYPVLARQLFELALEIREETGV
                     TLDFINLSGGIGVNYRPEQEPNDIAVIGEGVRKVYEEILTPAGMGHVKIFTELGRFML
                     APHGHLITKVLHRKETYRTYIGVDASAANLMRPAFYGAYHHITNITRPDAPIEVVDVA
                     GSLCENNDKFAVNRELPRVEVGDTLVIHDSGAHGFSMGYNYNGRLRSSEILLQEDGTA
                     RMIRRAETPEDYFATIYGFDFDR"
     gene            70268..70504
                     /locus_tag="AC1PIW_000070"
     CDS             70268..70504
                     /locus_tag="AC1PIW_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HIP6605048.1"
                     /translation="MNLGLAILLIVLAFAGGVALGIYLSRRQVENYIADKPILDENAL
                     RLMMSQMGQKPSEAKVQQVLRQIKSQQKVASKKK"
     gene            70594..71388
                     /gene="racE"
                     /locus_tag="AC1PIW_000071"
     CDS             70594..71388
                     /gene="racE"
                     /locus_tag="AC1PIW_000071"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HIP6605049.1"
                     /translation="MDNRPIGFLDSGVGGLTVARELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKNVKMIVFACNTATAVVWEEVKEKLDIPVLGVILPGASA
                     AIKATQTGKVGVLGTAMTIQSDIYREKIQALSPETQVDSLACPKFAPLVESNSHQSSL
                     AKKVVYETLRPLVGQVDTLVLGCTHYPLLRPIIQNAMGKDVKLIDSGAECARDISVLL
                     NYFQINRSRTEKDIQHRFYTTASPAAFKEIAESWMGIDIHVEHVEL"
     gene            71385..72395
                     /locus_tag="AC1PIW_000072"
     CDS             71385..72395
                     /locus_tag="AC1PIW_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HIP6605050.1"
                     /translation="MTDKIYEYRDEHNWFIGKASFANLFGSFGENGREQEIYQIGQLF
                     DKLIAGNYEDENFNQCVNIEVIKLQSEFALFQFACDTLNELNNRQFKALQHQGAILVT
                     ENDKLLLVHLPQAGVSTADFFGQDKGLASVGDTILIATKNEGKTKEFRKFFERFGYQV
                     ENLNNYPDLPDVAETGMTFEENARLKAETIAELTGKMVLADDSGLNVDALGGLPGVWS
                     ARFSGPDATDELNNAKLLHELAMVFELKDRSAQFHCTLVMAAPNRDSLVVEADWEGFI
                     GMDLRGENGFGYDPLFLVGETGKTSAELTLEEKNKISHRAQALEKLVEAFPVWQEQAK
                     QS"
     gene            72368..72895
                     /locus_tag="AC1PIW_000073"
     CDS             72368..72895
                     /locus_tag="AC1PIW_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIP6605051.1"
                     /translation="MAGASKTILVMSDSHGDRQIVEDIKHHYLSKVDAIFHNGDSELD
                     SQDSLWDGIQVVNGNCDYFGGYPDQLITQLDGVTIAQTHGHLYGINYGWQRLDYWAQE
                     VDADICLYGHLHVPDAEVRGKTLFLNPGSVSQPRGLVRECLYALVTIYDDHFHVDYYN
                     RQHQLYPALTKDISR"
     gene            72892..73350
                     /gene="cbpB"
                     /locus_tag="AC1PIW_000074"
     CDS             72892..73350
                     /gene="cbpB"
                     /locus_tag="AC1PIW_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HIP6605052.1"
                     /translation="MIAREFEAFLLDQEETFLTPADKLAVIIDTHNIDHAKLLLSHMT
                     YSRVPVVTEDGRFFGTIGLTEIIKYQAENELSDDELNRDISVIVKTDEETVGLDYDLT
                     EVMRKLVDQSFLPVLGENREFMGIITRKSILKAINALLHNFPLASKEGKK"
     gene            73347..74078
                     /gene="xerD"
                     /locus_tag="AC1PIW_000075"
     CDS             73347..74078
                     /gene="xerD"
                     /locus_tag="AC1PIW_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HIP6605053.1"
                     /translation="MKQAIESFIQSKKVSVNSQKSYTYDLQQFVTVTKGEISQQSLLV
                     YQQSLLDLKPAAQKRKMSAVNQFLYFLYENNLLDRFYKLQTTSGPASVKKKLEREDLT
                     LLFQESPWLEGQLIALLIAYLGLTPSEIAELTSQQVNLDFQVLTVEKGGAKRVLTLPK
                     ELIPYMESHLSGRYVFDKKGQTYSRQWFFNRLTEFVQSIGKPDWTAQKLREQYILKQI
                     DEGKSLDQIAKQLGLKTSMSLEKFR"
     gene            74078..74797
                     /locus_tag="AC1PIW_000076"
     CDS             74078..74797
                     /locus_tag="AC1PIW_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HIP6605054.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQVDIYEVPITEVIEQYLAYIAT
                     LQAMRLEVAGEYMLMASQLMVIKSRRLLPKVVEQIDPEDDPEMDLLDQLEEYRKFKLL
                     SEKLGEQHDERANYFSKPKLDLIYDDVQLAKDKTVIDIFLAFSKVMAEKQASLRQSHA
                     TIARDEYKIEDMMDFVRSRFETGPRLELRQLFQESQDVNEMITIFLATLELVKVHEIV
                     LEQTETFGDIYLVRSEDESLS"
     gene            74781..75350
                     /gene="scpB"
                     /locus_tag="AC1PIW_000077"
     CDS             74781..75350
                     /gene="scpB"
                     /locus_tag="AC1PIW_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIP6605055.1"
                     /translation="MNRLAEIEVLLFVAGEDGLTLRNLAEMLEMQPTAVSQQLEKLSE
                     KYMADKASGLAILESSNRYKLVTKKEYANLLRIYAKTPINQTMSRALLETLSIVAYKQ
                     PITRIEVDDIRGVNSSGAISKLQAFDLIRENGKKEVLGRPNLYVTTDYFLDYMGINSL
                     EELPDVSEIDLIQEETELFVERNELENEN"
     gene            75340..76071
                     /locus_tag="AC1PIW_000078"
     CDS             75340..76071
                     /locus_tag="AC1PIW_000078"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIP6605056.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELGTVIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVSDDKGRKTVVDLLPTVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYLARVKGVANKEVLRPLTRGVVIDGKKTKP
                     AVYEIIKVDPVKNRSVVELTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGNLDLTGLRP
                     GEYRKLNKKDISKLHTLAVTKPVKK"
     gene            76071..76310
                     /gene="yidD"
                     /locus_tag="AC1PIW_000079"
     CDS             76071..76310
                     /gene="yidD"
                     /locus_tag="AC1PIW_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HIP6605057.1"
                     /translation="MTRLLIGLVRFYQKFISPLFPPSCRYRPTCSAYMIEALQKHGLK
                     GFLMGLARIGRCHPLVEGGEDPVPDRFSLKRNKQK"
     gene            76879..77424
                     /locus_tag="AC1PIW_000080"
     CDS             76879..77424
                     /locus_tag="AC1PIW_000080"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0141098 - tRNA
                     (cytidine(34)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HIP6605058.1"
                     /translation="MNIETLEYIEETAKNHIVLFEPQIPQNTGNIARTCAATNSPLHI
                     IKPMGFPIDDRKMKRAGLDYWDKLDVRFYDNLAEFMDYASKQGRVHLVSKFADKTYSD
                     EDYNDGNIHYFLFGREDKGLPEDFMREHPEKAIRIPMNDEHVRSLNVSNTACMIVYEA
                     LRQQQFAGLDLVHTYDGDKLK"
     regulatory      77474..77688
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            77747..78304
                     /locus_tag="AC1PIW_000081"
     CDS             77747..78304
                     /locus_tag="AC1PIW_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIP6605059.1"
                     /translation="MTNTRKMTIIAILSAVSFLLMYLKFPLIPTASFLEVDFSLVPIL
                     FGLLILDMKSSFAILLVRTFLKLILNNQGPSTIIGLPMNIAAMSVFILAVAYFWKNEQ
                     TLKNYLKAAAVATIGSTAVMLALNYVYAVPVYAAFANFDIKEILGLTNYLFMMVLPFN
                     LLQGIVLSAIFYICYKAAQPILKKV"
BASE COUNT        22776 a        14792 c        18082 g        22741 t
ORIGIN      
        1 gatcagctta ttactcttca tcagcgaaaa tgagatttcg agtaaaaaat gaaacaacat
       61 gaaaggatta caaaaacatg tcaaaaacta ttcaggccat tactaatcag atttggtcca
      121 tggccaatga attgcgagga aatatggatg cttcggaata taagaactat atcctagcct
      181 tcatgttcta tcgctatcta tctgagcatc aagagcgttt cttggtggaa gatgaaatta
      241 tcagtcctgc tccaggagaa actgttcagg atgcctatgc gcgagaagca gttgggaatg
      301 acttggtaga gcatttggaa aatacagctt ctcaccttgg gtatgccatc gagcctgaag
      361 atacctgggc aagactggta gccaaaattg ataacagtga gattgtagcg agtgactacc
      421 agacgatttt cgatcatttt aacgccaatg tagaactcaa tcgggatgct atggaagatt
      481 tcagaggcgt ctttaatgac atcaatctag gggactctcg tttgggcaat tcaacggttg
      541 tgcgtgcaaa atccttgaac agtattgtga agttgattga tagcattgag tataaaaatg
      601 acgaaggaaa ggatattctg ggagaaatct atgagtatct tattggacag tttgccgctt
      661 cggcaggtaa aaaaggtgga gaattttata ccccgcatca agtgagtaag attttagcca
      721 agattgttac tttaggtttg gaaaaatcag atactagctt ctctgtttac gatccaacca
      781 tgggttcagg gtctttgcta ctaacagtgc gtaatgaact acctcagggc cagcatatca
      841 aattctatgg ccaagaaatg aacacaacta cttataactt ggcgcggatg aacctgatga
      901 tgcatcaggt cggctacagc aatatgattt tgaataatgc tgatacctta gaaagtgact
      961 ggccggacgg ggtggatgaa cttggaatcg atcagccacg tagttttgat gcggttgttg
     1021 caaacccacc ctactctgct aaatgggaca atcgtgagtc caaattgaag gatccacggt
     1081 ttatggaata cggtaagtta gcaccagctt ctaaagcgga ctttgccttt atcttacaca
     1141 gtctttatca tctgaataat acagggacca tggccattgt tttaccacac ggagtgctct
     1201 ttaggggagc agcagaaggt catattcgca aactgattat tgagaaaaat tacctggatg
     1261 cagttattgg cttgccagct aacctgtttt atggcacagg cattccaacc actattctag
     1321 tcttcaaaaa aaatcgtcag accaaggatg tcttctttat cgatgcgagc aaggagtttg
     1381 agaaagggaa gaatcagaat cacctttccg atgacatggt agaaaaaatt gtagagacct
     1441 atcacaatcg tcagtctgtt gacaaatatg ctcatttggc atcaatagaa gaaattgtag
     1501 aaaacgacta caatctaaat attcctcgtt acgtcgatac ttttgaggaa gaggaagaga
     1561 ttgatctcgg gcaagtaacg cagcaactag aacaagatag actagagatt cgggctctag
     1621 aagataagat tcgtcaacaa ttaaagactt tgggagtaga tttttagata aatgaaaaga
     1681 gcgagtagga tttttcctcg ctctttcttt gtatggctta aggtttaatc acttctaccc
     1741 cacccatgta tggacggaga acttctggaa tggtgacaga accgtcttca ttttgatagt
     1801 tttcaaggat ggcagccact gtacgaccaa ccgctagacc agaaccgttg agggtgtgga
     1861 gcaatttgac tttaccgtca gcagcatcgc ggtagcggat ttgggcgcga cgagcttgga
     1921 aatcttctgt gttggaacag cttgaaattt cacggtaggc attttgggca ggaatccaga
     1981 cttccaagtc ataggtctta gcagctgaga agcccatatc gcctgtacag agggccaaaa
     2041 cgcggtaagg caagcctagt ttttggagaa tattctctgc gtttgctgtc attttttcca
     2101 attcatcata agactgttct ggtgtggcaa atttgaccat ttcaaccttg tggaattggt
     2161 gaaggcggat aagaccacgg gtgtcacgac cggcagaacc agcttcagaa cggaaagatg
     2221 ggctcatggc tgtgaagtag attggcaggt ctttttcttc cagaatctcg ttgcggtagt
     2281 agttggtcaa cggcacttcc gcagttggga tgaggacgaa gttagtgtct gccaactcaa
     2341 aagtatcttc cttgaactta ggatattgac cagtaccaaa catagagtcg tggttgacca
     2401 tgtaaggcgt gatgatttct tggtagcctt ctgcgatatg ctcgtccaac atgaagttgt
     2461 agagggcacg ttctaagcga gcacccaagt tcttgtagaa gaggaagcgt gcaccagtta
     2521 cttttgcgcc acgttcccag tccagaatat ctaggtcttc gcccaaatcc cagtgagctt
     2581 ttggctcgaa agtgaattca cgaggtgttc cccaacggcg aacctctacg ttttcttctt
     2641 catccgcacc aactggaaca gaatcgtgcg gcgtatttgg aagaacggtg atgatggcgt
     2701 ttaattcttc atcgatttcc aaaagctgag cgtctaagtt tttcacatcc gcagacagtt
     2761 tctgcatggc agcaatcttg tcgtcagcgt tttccttatt gcgttttgct tgggcaatct
     2821 ccgcagaaac ggtgttgcgc tctgctttta attcttctac agtaaccaag atttcacggc
     2881 gttttgcatc caattccttg atgtttgcca aggtttcagc agccacacca cgagtagcta
     2941 atttggcagc cactccgtca aaatcagtac gaatacgttt gatatctagc atagatgtct
     3001 cctttagaat aaaaaagcac agaatgagag atgctggagc gatatttacc gcggttccat
     3061 ccagcttcac attctgtgca cttgattgta ttttgataat ttcataacgg tagaatttca
     3121 caactttttc ctatctgctc acagcgaccg cagactttct ggaaggagcc agatgctact
     3181 tatccgtttc tactattata cttgaatttt tattttttgg caaatagatt cgaaattttt
     3241 ctgccgattg tttgcataag agtcggtaat ttcacaactt tttcattgcc gatatgctca
     3301 cgggcaattt taagaatctt accagaatct ttagatgcaa acaagaatcg acttttctct
     3361 gtataaattt caaaatgacg gctgattgct ttgccagaaa cgtttgcgcc aatgctagtc
     3421 atttctgacc aaggaatctg gatatagcgt tcaacgttgc tatctgggta gaattcaagt
     3481 gccttgtcac caattagaaa tttaccaacc ttgccaccaa agcccatgta ggaaacccct
     3541 gtggtttgta gttcaacgct tgtattttgt gattgtgcca tagtgctctc tccctagata
     3601 ttcattatat cataaaaccc cgcaagagcg gggtccttga ttacataatt ccgaagaaac
     3661 gagcaagaat acctacgatg aagagaccga taatcattgt gattggtgtt actttcttct
     3721 taagcaagtg catgcagagg aaagtaagga gaagtcccat caaaccagga atcaagctgt
     3781 ccaactgacc ttgtaatgtt tgagcttgag ttggttcaag gctaagtccg ctaagagctt
     3841 gaccaagaat acctttcaac tcagcaccag taacagtacc ttctgggaag acgatgtaag
     3901 caccttctga caattgttta gcaggaagat ctacagcgaa agtaatagat acccaacgtt
     3961 gaacaagaac ggcaaggatg aacataccaa ggattgaagc acctttagtg atgtcttgaa
     4021 ggataccacc agacatgtct ttagtgattt cgctaccagc tttgtaacca aattcttgtg
     4081 tataccatag gaaggccata cgaatgatgt tccagccaag gaagaagatg attggtccca
     4141 tgatatttcc agatagtgca agtgaagcac caagagcacc aaggattgga cgaacagtaa
     4201 accagaagac tgggtcaccg ataccagcaa gaggtcccat cataccgatt ttcacacctt
     4261 ggatagctgt gtcatcgatt tcagcaccat tagcacgctc ttcttcaagg gcaagagtta
     4321 caccaaggat tggtgaagca acgtatgggt gtgtgttgaa gaattccaag tggcgctcaa
     4381 gagcagcagc ttgatcttct tttttagtgt aaagtttttt gatagctggg atcattgcat
     4441 aagcccaacc taagttttgc atacgctcat agttccatga accttgaagg aaggttgaac
     4501 gccaccaaac ttttttacga tcgcttacgg aaagttgaat tttttctgac atgatgaatc
     4561 tcccttctct tagtagtctt caaggatgtc gccgattggg tcgttagatg aagcaccacc
     4621 gttacctgat ccgccttgtt ttgacaagtt gaggtagata agagcgattg caacaccgat
     4681 aacaccaagt gcgataagtg tcaatttgct gatagcagca aatgcaaaac caagtgcgaa
     4741 gaatggccat acttcacgag ttgccatcat gttgataacc atagcataac ctacggcaac
     4801 aaccatacca ccaccaacgg ccataccacc tgacaaccaa gctggcatag attcaagaac
     4861 tgcttgaaca gcttctgcag gaattgcaag aagaagagcc gcaggaactg cgatacgcaa
     4921 accttgaagg agaagagcaa acaagtggta acgttctaca gcagcgaagt ttgcatcttt
     4981 agcagcattg tcagcaccgt gtacaagacc gactgagata gtacgaacga tcattgtaag
     5041 gaaaagacca gctacggcaa gagggatagc agttgttgtt gcaacggcaa taccttcagt
     5101 tgagaagtcg ccacctttga tcatgatgat tgcagcagct acagaagcaa gagcagcgtc
     5161 aggtgcaaca gctgcaccaa tgtttgccca accaagtgca atcatttgta gagaaccacc
     5221 aagcataaca cctgcagcca agttaccagt cacaagaccg ataagggtac aagctaccaa
     5281 tggttgatgg aattggaatt cgtcaaggat accttcaaga ccagcgaaga aggcaacgat
     5341 gacaacgaga attgcagaaa taagtgtaat atctgacatg ttgtaatcct ttctatacta
     5401 acagatcgat ttattgaacg tttgccttgt tgataagttc aaacaagtct ttcttcgaat
     5461 cgtttggtac tttacgcaca tcaaattcaa cgccaaggtc acgcaatttt tcaaaggttg
     5521 caacgtcatc tttgtccatt gacaatacgt tgttaaccat tgttttacca gttgagtgag
     5581 ccatagaacc aacgttcaat tctttgattt caacaccacc ttcaattgct tcaagagctt
     5641 cttgaggagt ttcgaaaaga atcaaggcgt gtgtgttacc gaatcgaggg tctttagaaa
     5701 cctcaatcaa tttcttgatt gggacaacgt tcgcttttac gttaccaggt gcagcttgtt
     5761 tgatgagttg tttgcgcaat tcatctttgg caactgtatc tgatgcaacg atgatacgat
     5821 ctgctttgga agcaggagtc caaccagtcg ccacttgacc gtgcaagaga cgagtgtcga
     5881 tacgggctaa gttgattttc aacttgccat caccgataac agtacctgga gggatagcac
     5941 cttgtagtgc aacaggagca gcatcggcaa caggagcagc ttcagctggt tgaagttctt
     6001 ctggaagaac tttgatacca tctttggctt ccttgataat gtttgccgca acttcttcca
     6061 cgccagcagc agcgttaatc atacgctctg tgtaagcttg gataagcatt ggcaagttta
     6121 gaccggtgat gatagcaaac ttacgatctg gattttcacc agctacacgg cttgcttggt
     6181 tgaatggtga accactccaa aggtcagcca aaaccaaaac ttcatcatcg gcatcgaatg
     6241 cgtccacggc agcatttagc ttagcgtata aatcatctgg accttcactt ggcataaaag
     6301 taacaacttg tactttttct tgttcgccaa agatcattga acctgactga tgaataccag
     6361 cagcaaattc gccatgactt gcaataatga ttccaatact cattattgat cctcctaaat
     6421 ttttcttgtt catgtaaaaa ccattgaatg ggctttcatg ttcgaactgt accattataa
     6481 aacgttttca gtacatttgt caagtaggtt tgtgttaaaa gtgcaaaata tttccgaaac
     6541 ttttgataat cgtgtttcag aaaagtgata aaaatatgca acgaatatga caaaaacctg
     6601 atagtttatc aggttttttg atgtctataa aaataggttt tctagttcac gtgcgacacc
     6661 atcttcaaaa ttagcaaatg ctgtcaatct gtcggcatgc ggacgaagtg tatcgctaca
     6721 atttttcatg gcataggctg ttcctgctag gtctagcatc tcaacatcgt tgtgctcatc
     6781 gccgaaggca attaagtcgc taggagaggc ttggtagagg ttaagaacat agctcagagc
     6841 ggttgcctta ttgacgcctt ttgcacaagt ttcgaggata tttaatggtc cgccccacgt
     6901 attgatttct aattcatctt tgaaataggc attcatttct tttgcaagtt catacttatc
     6961 agttgcacga gtctgcataa ggatggaatg aggatcacta gtgattaatt caggtttgat
     7021 gagtgtatca ggagtaatct gttcaactcc catcagagca ggatcaatct tgtctaagtt
     7081 cttttgagtg atgtagaatt tattcttgta ttcaccagca ataaaatctg cctcgaatgt
     7141 ttcttcttct tttagaaatt cgagtaggta ttttttgtcg attaaaatat tttgttccca
     7201 ttgccatttc tttccaggga tgtgaactaa agaaccgttg aaattaatca tgggagtatc
     7261 cagttctagt tgttggtaaa atttttcagc cattcgatag gggcgacctg tggcgataag
     7321 aacggtgtgt cctgcttggc ggaccttctt tatagttgag atggtataat cagataattg
     7381 gctatcatta ttgagcaatg tgccgtccaa atcgatagct atgatttttt tcttcatatt
     7441 taatagcatt cctttctaga gttgttatac tagtttttaa tagtgaataa tgtttaggta
     7501 gtagttagag tgctgagaaa ttgttttatg cgtcaaagaa tagtacttgt aggtagccac
     7561 atcttgaact aataacttcc agtataacaa aaggagataa taaaagcaaa gaagcaagta
     7621 attttgtata gatttttgca agattgcaac gttcggaatt tacgaatgtt taaagttgaa
     7681 aattaataaa ataacgtttt caagattaaa aaacttgaaa ttataatgtt ttttgattat
     7741 aatgttttta ttgttcgtaa attaaaagga gagatgaaaa aatggatcaa ttttttaaat
     7801 tgaaagaaca tggaacgact gtttctacgg aaattatggc aggacttacg actttctttg
     7861 ccatgtccta tattttgttc gttaacccga gcgtattgag tgtggcaggt atgccaacac
     7921 aggctgtttt cttggctaca ataattgcca gtgctgtatc aacgcttgtt atgggattat
     7981 ttgctaacgt accttacgcc ttggcaccag gtatggggtt gaatgccttc tttacctata
     8041 cagtggttat tggtcttggt tttacttggc aagaagcttt ggcaatggtt ttcctctgcg
     8101 gtttgtttaa cgtttttatc acagttacaa aggttcgtaa gagtattatc aaggcgattc
     8161 cagttagttt acaacatgcc atcggtggtg gtatcggtgt attcgttgcc tatcttggtt
     8221 ttaaaaactc taatttaatc accttcttga catctggttc agatattatt actgtaaatg
     8281 gtgttgctcc ggcagatgca acagcagaaa cattctcaaa cggtgttttc tcagtttttg
     8341 ctggtggtgg tgtcgtacct ggtatttcaa cctttactga tccaagtgtt ctcttgacag
     8401 tgttcggtct tcttttgaca gctgttttgg ttattaagaa cgtgcgtggt gcaattttga
     8461 ttggtattat tgcaacgact gtagctggaa ttccagcagg tgttgtggat ctgtctacaa
     8521 tcgattttgc aaataacaat accggtacag cctttgcgga gcttggtaca actttcctag
     8581 cggcttttga tggtctatct tcgctctttg ctgactcaag ccgtctacca cttgttttga
     8641 tgactatctt tgcattcagc ttgtcagata cctttgatac acttggtacc tttatcggta
     8701 caggtcgtaa gactggtatt ttctcagaag aagatgaaaa agctctcgaa aatggtacag
     8761 gctttaactc taagatggac aaggcgctct ttgcagatgc gattggtacc tctatcggtg
     8821 cactatttgg tacgtcaaat acaacgacat atgttgaatc ggctgcaggt atctctgcag
     8881 gtggtcgtac tggtctgaca gctgttacaa ctgccgttct cttcttgcta tcaattttga
     8941 ttttaccatt cattggaatt gtgccaaatg ctgcaacagc tccagccttg attatcatcg
     9001 gtgtcatgat ggtttcatcc ttccttgatg ttgattggag ccactttgaa gatgcgcttc
     9061 cagccttctt tgccgccttc ttcatggcgc tttgcttctc tatctcttac ggtattgcag
     9121 ccgcctttat cttctattgc ttggtaaaag tttcgacagg taaggctaaa gaaattcatc
     9181 caatcctttg gggtgccaca gctctattta ttctgaattt catcattcta gccatcttgt
     9241 aatctgttta ttatccttga actgatcttc atttgaaggt cagttttttt atttacgatt
     9301 aaaatatatg ggaatcaatg tgatttattc agcataatgc tgtattgaag attgtaaatt
     9361 gaaaacggat ggagtttatt tttccatttc gtcgcttctc tgtctcctct tagtgccatt
     9421 tttactcctt gtgactgtat tttaaatggc tgttcgttat tgtaagcaag atgtctcata
     9481 cctagacatc cgctggatta atgtcatgat ggggtgaaaa atttatcatg tagtctttgg
     9541 gagtcacagg gcatagaata acccctgagc ttgggctcag ggggagtttt ctaactataa
     9601 tgtttgaata gcgaatgagt tgcctgatgg atggtgcgtg ctgtttcagc gttgttcata
     9661 gtgtagagat ggataccagc cacatcattg gtcactaagt caacaatttg atctacagca
     9721 taggccagac ctgctgcccg aagagattca ggattgtgct cgtatttttc caagatggcc
     9781 ttgaatttac gagggaggcg gatattctca caggttttca agagtcgaag tgcctgattc
     9841 ttgttgataa taggcataat acctgcgata ataggcactt cgatgtctgc tagagaacat
     9901 ttctcctgaa agttataaaa tgcttcgtta tcgaagaaga gttgagttac caaggttgag
     9961 caaccggcat caactttctt tttcaaattt ttgatgtctg agacagaatt tggtgattct
    10021 ggatgtactt ctggataaca ggctccaata atgtcaaaat gtggggcttc ttccttgata
    10081 aaggaaatca agtcagttgc atactgaaag tcctctttag ggggaagacc ttcaataata
    10141 tcgccacgaa gtgctaaaat ctgatgtaca ccaatttcat cgagagaatg aagaacggat
    10201 cggacctttt cttttgtcaa gtaagcagct ggcaaatgtg cgatagttgg aatatgcaat
    10261 tcattgcgaa catggttagc caacttgaca gtagtctctt ctacgttcaa attgttattg
    10321 ctgcatgtaa cactgataaa atgaggggcc aaaccttgca tctcatcgag agtttggata
    10381 atcttttcat tgcctactgc tggttttgga ggaaaaattt caaatgagag acttggggtt
    10441 tgaccaatca taaattagag ttcctttctg gctgccttag ctgcttgagt caagtggata
    10501 agactagctt ttgtttctga ttcgccacga gtcttgagtc cgcagtctgg gttaatccaa
    10561 actttttctt tcggtacttt agcaaggatg gcttgaatag tgtgtgcaat ttcatcaacg
    10621 gcagggacac gtggcgagtg gatgtcatag acaccaggac ctacctgagt ttggaagttc
    10681 ttagccttaa gttcatctag gataacaaga tttgaacggc tggcttcaaa ggagataacg
    10741 tccgcatcca tattgtcgat ggcaggaatg atgtcagtaa attcgctata gcacatgtgg
    10801 gtgtgaattt gtgtatctgg tgcaacagtt gagtgtacta aacggaaggc agggattgcc
    10861 caatcaagat actcgctgta ccagtcgcta cgacggagtg gtagtttttc acgaagggct
    10921 gcttcgtcga tttggataat cttgataccc gctgcttcaa ggtcaagaac ttcttccttg
    10981 atagcaagag caatttgaag ggttgattct ttgatagaaa tatcttcgcg tgggaatgac
    11041 cagttgagga tggttacagg acctgtcaac atacctttaa ctggtttgtt ggtacggctt
    11101 tgagcatagc tagaccattt gacagtaatt gggttcaagc gtgttacatc accccagata
    11161 attggtggtt taactccacg cataccgtag gattgtaccc aaccgttttt actgaagagg
    11221 tagccagaca ggttttcacc gaagtattct accatgtcgt tacgctcgaa ttcaccgtgt
    11281 acaagtacgt cgaagtcaac ttcttcttgc catgcaatcc actcatctgt ttgagttttg
    11341 acaaatgcat cgtattcttc ttctgagatg tttcctttgc gaaaggccaa gcgtgtgcta
    11401 cggacttcct tggtttgagg gaaggaaccg atagtggttg ttggaagaag tggtaagttt
    11461 agcttctcgc gttgaatgga ttcacgctct gcaaagactg gtaggcgtgt atagtcagtg
    11521 tctgtcagtc ctgcaagacg gtctgcaagg gctgcatcac gaccaacacg ctcaagggca
    11581 aagagttcct tgttagctgt gagagcaact tcgccttgac cgttacggat ggcatccaaa
    11641 tcacgcaatt cgctcagttt ttcaactgca aaggcaaagt ggttgaggat agctggttca
    11701 aattcttcat ttgctgttgt gaaaggtaca tgaagaaggg agcaagaagt tgttataacg
    11761 acattttcag ctggaatagc atccaaaaca gccaggctct tttcgtagtt gttgcgccag
    11821 atatttttac cgttgacgat accagcgtag agggtcttgt cagcagggaa accagttgca
    11881 acaagtgcag ccgtttcctt gccttctaca aagtcaagac cgatggcatc aactggtagg
    11941 ctggtcaatt ctgtgtaaat atcacgaaca tcaccgaagt aggtttggat gagaacttgc
    12001 aaacctttct tatctgccaa gagtgtttgg tagatacgta ggaaaagggc tttttcttca
    12061 gcagtcaaat ccttgaccaa gctaggttcg tccagctgga ttttttcagc accaagctct
    12121 gctaattttt caaagacttg tccgtaggct gcaactaagc tatcagcgaa attagtagca
    12181 gttaaacctt cttcaaaatc agttaattgt aagagtgtga atggaccaat caaaactgga
    12241 cgagttgtga tgcccaagtt ctttgcttct tggaattcat cgaaaatctt atgaccggct
    12301 agtttgattt ctgtatcttt ttcaatagct ggaacgatat agtggtagtt ggtgttgaac
    12361 cattttttca tcggtctagc acgaacgtcc cctttttctc cttgataacc acgtgcaaga
    12421 gcgaagtatt tttcaaggtc tgttaattca aggtcttgaa cagctttggg tatgatgttg
    12481 aagagtactg ccgcatccaa aacattgtca tagtgtgaga agtcgttgct tgggatttca
    12541 gtaatgcctg cttctttaac aatattccag tgcttagcac gtaagtcttt agctgcttcc
    12601 aaaagctctt cttgtggaat ttcatttcta aagtattttt cagtgataaa ttttaactca
    12661 cggtgttctc cgatacgtgg gaagccgata atagtagtag tcatggtttt tcctccaaaa
    12721 tatttctatg cccctatcct aacagaaaaa acgactcttg tataattgta aaaaattaga
    12781 gggagttata acttttgact ataacagatt gaaagagagc tacttttttg atatttcttc
    12841 tatataaaag cttgaaaaaa agcctgttta tcaagcttga cttttttatg ctggctccag
    12901 tatagttttt tcttaagcgt ggtatagatt ttatctatga aactgctaaa ggcaaactct
    12961 tgtttatgag caattgttta taggtagttc tgatagttat tttttatcaa gccataaaac
    13021 ttctatatta gacagggcta gatagccatg ctacaattga aaaaatgaaa gaaaggggct
    13081 ttttatggga aaagttatca ttggaatttc aggaaacgag caagaatttc caacaaaatc
    13141 tggtcgcgta tatgtaacgg tagcgcgtga attggcagac agcgttcgtc aggctggtgg
    13201 tgtaccaatg gttattccta tgggaactcc tgacttagcc aaggattata tcgatatgat
    13261 tgacaagttg atcttgtcag gtggtcagca tgtggatcca agtctctacg gtcaaaaacg
    13321 cttgattgat agtaatgatt acctcttgga acgggatgaa tttgaactgg ctttgattga
    13381 ggaagccctt catcagggaa aaccgatttt tgcagtctgc cgagggatgc agttgctgaa
    13441 tgttgctcta ggtggaagct tggaacaaga ggtggatcat cattggcaag atggtattgc
    13501 tgggacatcc catcgtttac aagtgaaacc taaaagccga atcggtcaat tgtttgccca
    13561 agggagccct attaattcct tccatcagca acgaatcaaa gaattggctc caggactggt
    13621 ggcgacaggt ttggatccta gagatggtac gatagaagct tatgaaagta gagggaacca
    13681 agctttgttt ggtatccaat ggcatccaga atttttatac aatgattgta agcaacatcg
    13741 agacctattt cagtatttag tagatatatt ttaagagggg tatccctctt tttattatca
    13801 atacttataa ttgcaagggt atttgttata acgaaaagtt ataactttct ataaaaaaag
    13861 tcaattagtc agaaagaaaa ttctgtgtta agatagatac agttaattta aggagatcat
    13921 tttatgaaat taaaaaaatt gtttagtttg gcaacggctg ctttatcagt aggtgtactt
    13981 gctgcatgtg gtaactcttc ttcaagttca gattcatctg caacaactgt tcgagtaggt
    14041 gtgatgagct tgagcgattc tgagcaagcc cgttgggata aagttcaaga aattcttggt
    14101 gatgaagtga aattggagtt cactcaattt acagattact cacagccaaa caaggcagta
    14161 gctgaaaacg aagtagacat caacgccttc cagcactata acttcttgaa taactggaat
    14221 caagaaaatg gtgaagactt ggttgctatt gcggatactt atatcgcacc aatccgtctt
    14281 tactcaggta cagctgatgg aaaaaacaag tacactaaag tggaagaaat tccagatggt
    14341 gcagaaattg cggttccaaa cgacccaaca aacgaaagtc gtgctctcta ccttcttcaa
    14401 gcagctggtt tgatcaaggt tggtgtatct ggtacagaat tggcaactat tgctgacatt
    14461 actgaaaaca agaaaaactt gaagatcact gagttggatg caagccaaac agcaagctca
    14521 cttagctcag ttgatgcagc agttgtaaac aacacatttg tgttggaagc tggtttggat
    14581 tacaaaaatg ccctttacaa agagcaaaaa gatgaaaatt caaaacagtg gtacaacttg
    14641 attgcagctc gtagcgattg ggaaaaatca gagcaagcag cagcgattaa gaaaattatc
    14701 gaagcctacc acacggatga agtgaaaaaa gttattgaag agacttcaga cggtatggat
    14761 gagccagttt ggtaaaatgt aaataggttg aggactgggc agaattgttc agccctctat
    14821 ctgtttttat agagataaga aaaaatctct taaccaaagt cagaaatgtg ggagaatatt
    14881 tgattttggt tagtagattt aattggtttt cattgagtat gaggagaaag tatggcagat
    14941 agtaaagttc aagcatttga aaatgatgcg attattcaaa cctattttga aaagttgaag
    15001 gtcttgattt caaagaaatc catttttgct cagcagattg gtttgctgga tgtggcgact
    15061 tacttgaaag atatatttga agaagctggt gcagaggttg ttttggatga tagttatgca
    15121 gcaccctttg tcatggctag gtttgaggct tcggttccag atgcgaaaac attgattttc
    15181 tacaatcatt atgatacagt acctgcagat gccgatcagg tctgggagaa gggcaacccc
    15241 ttcgaattga ccatttccga tggctatatc tatggtcgcg gggtagatga tgataagggc
    15301 catatcacag ctcgtctgtc ggccttgaaa aaatatcaag ctagacagaa tggccattta
    15361 ccagtcaatg tgatttttat tatggaaggg gcagaagagt cagcctctgt cgacttggat
    15421 aagtatcttt caaaatataa agaacatttg attggtgctg atttgcttgt ctgggagcag
    15481 gggcatcgca atagcctaca tcagctagaa attgctggtg ggaataaggg aattgtgacc
    15541 ttcgatcttc aggtcaaatc agctgacttg gatattcatt cttcctatgg tggtgtcatt
    15601 gattcggcaa gctggtattt attatcagca cttcaatcca tgcgggctgc agatgggcaa
    15661 attctggtag aaggcattta cgatcaggtt caagagccaa atgagcgtga attggccttg
    15721 gtggaagaat ttgcactggc taccagtcag tctatgacgg atatttatgg actaaccctt
    15781 ccaaccttga tagaagatcg tcgtgagttt ctgaagcgtt tgtattttga accatcgatt
    15841 accattcaag gcttgtcaac tggctatctt ggtcaaggtg tcaagaccat cataccagct
    15901 caggcttctg ctaagatgga agtccgtctg gtacctggat tggagccgca tgatgtatta
    15961 gataagattc gccagcattt ggataaacat ggttttgaca aagttgaggt tgtcttcact
    16021 ctaggagaga tgagctaccg aagtgacatg tcccaccctg ccattgtcaa tgtgattgag
    16081 ttggctaaaa agttgacgcc agaaggagtt gctgttttgc cgacgtcacc ggggacagga
    16141 cccatgcata cagtttttca tgccttgggc gtgccgattg caggatttgg attaggaaat
    16201 gccaacagtc gcgaccatgc aggtgatgaa aatgtcagca tcgctgacta ctatagccat
    16261 gttgaattag tagaggagtt aattgcaagt tatgagtaag gaaatgatta agctagataa
    16321 tattgatgta actttccagc agaaaaaacg tacgattgaa gcggtaaagg atgtgaccat
    16381 ccatatcaat caaggggata tttacggtat cgtaggttat tctggagcag ggaaatctac
    16441 ccttgttcga gtgattaatt tattgcaggt gccgacagcg ggtaaaatta ctatcgatga
    16501 ggatgtaatt tacgagggag aaaaggtcac acttacccca gctcaattgc ggagcaaacg
    16561 ccgtgaaatc ggcatgattt tccagcattt caatctgatg gctcaaatga cggcagaaga
    16621 aaacgtagcc tttgccctta agcattcggg tttgagcaag gaagagaaga aagaaaaagt
    16681 tgctaagttg ttggacttgg ttgggctttc tgaccgtgct gaaaactatc cagctcagtt
    16741 gtctggtggt caaaaacaac gtgttgccat tgcccgtgcc ttggccaatg accctaaaat
    16801 cttgatttca gatgagtcaa cttctgcctt ggatccgaaa acaactaagc agattttggc
    16861 acttttgcag gaattgaatg aaaaattggg cttgaccatt gttatgatta ctcatgagat
    16921 gcagattgta aaagatattt gtaaccgtgt ggctgttatg caggatggtc gcttgatcga
    16981 agaaggttca gttttagaaa tcttctctca tccaaaagaa gaattgacac aggatttcat
    17041 caaaacagca acaggaattg atgaggcctt ggtgaaaatt taccagcagg atattgttaa
    17101 gaatttgcct gagaacagta ttttggtgca actcaagtac gcaggttcaa atacggatac
    17161 ggcaattgtg aatgacttgt ataaattcta tcaagtatct gccaatattc tttatggcaa
    17221 tattgaaatt ttggaccata cgccagtcgg tgagatggtg gtgatcttat caggtgaacc
    17281 tggtcagttg caccgtgcga ttgaagcagt gacggaagcg cgtgtggaag tgacgatttt
    17341 gaaaggagcg aactagatgt tagaatggat tcaaacgaat tttccagata tttataaatt
    17401 gggctgggat ggtcagacag gttggttaac acattttaat ttgaccctct atatgacctt
    17461 tgtttccttt gcttttggtg gatttatggg cttggtgtct ggtttgttct tggtcttgac
    17521 aggtccacgt ggggttattg ccaataagac tgcctattgg attttggaca aggtggcttc
    17581 tatcttccgt gccattcctt ttattatctt gttggcggct attgctcctt tgacgaaaat
    17641 tattgttgga aaaaccattg gtacagaggc ggctttggtg ccgctcgctc tttcagtctt
    17701 tccgttcttt gcccgtcagg ttgaagtggt tttatcagaa ttggatcgtg gtgtcattga
    17761 ggcggctcag gcatcaggtg cgactttctg ggatattgtc cttgtctatc tgcgtgaggg
    17821 tctgcctgac ttgattcgtg tgacgacatt tgccctggtt tccttggtcg gctatacagc
    17881 catggcagga gctatcggag caggtggttt gggacaggtt gcgctatcat acggctacct
    17941 acggtataat aatgatgtga cctttttagc aactcttttg attttagtta tcatcttcgt
    18001 aatccagttt attggagatt tcttgacgag aaagattagt catagatagt tatttagttg
    18061 tgttttttca aattttggat agtccttgac ttatttaaag catttttata caataaattt
    18121 aattaaataa acactgccac cgggtagtca tgcaacagca ttttatgaac gtcgttaggt
    18181 cttagaagat gttcataggg tgctgatttt tctataaaag gagtgagaat atgtcacaat
    18241 ttgttgttga ttcatctttt tggagtctat ttccagatgc aaaaattgga gttctattat
    18301 tgaaagatta taaaacgccg gcgcaatcgc ctgatgaatt agtgaaatta ctggaagaaa
    18361 gcaacggaat tgcccagaaa tttcttacgg aagatacctt tagtgagaat gaggtcattc
    18421 gtacatatcg tcaagcctat caaaaattta aaacaaaaaa aggcgcgcgt tctagtatcg
    18481 aagcactttt gaaacgttca actagtgacc gtccagtcac aacaatttct ccattagtgg
    18541 acatttataa tgcggcgagt ttacgttttg gtcttccatg tggtgctgaa gatttagata
    18601 catttgtagg caatctccaa ctaacggtca ctgagggtgg ggatgaattc tatctcattg
    18661 gtgatgaaac caataatcca actttaccag gtgaagtttg ctataaggat gataaaggcg
    18721 ccgtttgtcg ctgtttcaat tggcgcgatg gtgagcgaac catgattacg gatgagacaa
    18781 agaatgcttt cttggtcatg gagttggtca attctgatag agtagaagat ttggaaaatg
    18841 ccctagattt tatcagtcaa cacgcagaaa aattcttggg agtagtacca gaaaaatatc
    18901 tactagattg cgataggcct gcaatagcct tgtaggattt gtcagcggga ttgtgttcgg
    18961 ataaatagtg gtctttctag acaaatgctg atttttctgc tacaatacta agcatgaaag
    19021 aaggttgttt caaaagcctg tctagcgagc ttgggagagt gaaagccaag ccagtcttga
    19081 aataattggc gctttcccag tcgtgaccat gtccgactca atcagaatca tacaaatgaa
    19141 gtaataaatt agggtggaac cgcgttctca acgcccctat gtctgttgac aggggaagag
    19201 aatgcggttc tttttgtctg ccgaccgagt atttcttgtt tttcatctca aggtgaaaga
    19261 cagctattat aaagaaagga gaccaatgtt caccttagaa aatatcaatc acgggcatga
    19321 acagtttaca gggcctgatt ttcccaaact gattgcctat tttaaggact tgggtatggt
    19381 ggaaaatatt gtggatatcc agtctggcca ggttacctat cgctcacaga cagggcaaac
    19441 tctggaaaag cagggctatc aagtgacgat acctgtttcg gaccaagcca atctggacca
    19501 gtttgtgact atcttacgca atcatcaggc tggacaaaca gattttctca ccttctgcca
    19561 agaaacagca gaagcaggga tttacaagtg ggtcatcgat ctagaagcca tgacctgttc
    19621 ctatatggac aaggcagaac aggctgtctt tgtggaaact ataccgagtg tggagaacta
    19681 gccattttgc aatatatata gaaagaggct gttatgtcaa agaaacttac ttttcacagc
    19741 gccagtggca tagtctcctt tcagtcgaat tgccacacgc tcatcgctat ggcggctgag
    19801 ctagacgctg tactagttcg cctttcctag cggtatcggc taggaaggct cacgtcgcaa
    19861 aaacatcgtc atttagttta tcttttatta cgtcgttaac tcgctttgcc gtacctcagt
    19921 actgcctgca gcttgttgcc tagtactaaa agtaaactaa aagacttaga tttaaagaaa
    19981 gaaggaaaaa tatgtcaaaa aaacttactt tccaggaaat tatcctgacc ttacaacaat
    20041 tttggaatga gcaggggtgc ttgctcatgc aggcttatga tacagaaaaa ggtgcgggta
    20101 ctatgagtcc atacactttc ttgcgtgcta tcgggcctga gccttggaat gcggcttatg
    20161 tagagccttc acgtcgtcca gcagatggtc gttatgggga aaacccaaac cgcttgtatc
    20221 agcaccacca attccaagtg gttatgaaac catctccatc taatatccaa gaactttact
    20281 tggaatcttt ggagcgtttg ggtatcaatc ctttggaaca tgacattcgt ttcgttgagg
    20341 ataactggga aaacccttca acaggctcag ctggtctggg ctgggaagtt tggttggacg
    20401 gtatggaaat cacgcagttt acctatttcc aacaggttgg tggtttggca acaggccctg
    20461 ttacagccga ggtaacctac ggtttggagc gtttggcttc ctatatccaa gaagttgact
    20521 ccgtttacga tatcgaatgg gctgacggtg ttaaatacgg tgaaatcttt atccagccag
    20581 aatatgagca ttcaaaatac tcctttgaag tatccgacca agacatgctc cttgaaaact
    20641 tcactaagtt tgaaaaagaa gctgagcggg ctttggaaga aggattggtt cacccagcct
    20701 ttgactatgt actcaaatgt tcccatacct ttaacctgct cgatgcccgt ggagccgtat
    20761 ctgtaaccga gcgtgctggc tacatcgccc gtatccgtaa ccttgcccgt gtcgttgcca
    20821 agacctttgt ggcagagcgg aaaaaactcg gtttcccact tttggatgaa gcaacacgag
    20881 cagaattgtt gaaggaggac gcagaataat gacaaagaat ttacttgttg aacttggctt
    20941 ggaagaaatt cctgcctaca tcgtaacccc agccatgcac cagttgcgtg accgcatggc
    21001 gacctttttg acagacaatc gcttggcttt tgacagcatt gatgtctttt caacgccacg
    21061 ccgtttggct gttcgtgtgc gtggtttggc agaccagcag actgatctta cagaagattt
    21121 caagggtcct gctaagaaga ttgccttaga cgcagacgga aacttcacca aggctgctga
    21181 aggttttgtc cgtggcaaag gcttgacgac agctgacatt gaattccgcg agatcaaggg
    21241 cgaagagtat gtttatgtga ccaaacacga agcaggccag ccagctaaga cagttttggc
    21301 gggcattcca gaggtcttga aggctatgac cttccctgtc agcatgaact gggcgtccaa
    21361 caaattcgcc tacatccgtc ctgtccacac cttgaccgtt cttttggacg atgaagcatt
    21421 ggatatggac ttcttggaca ttacatctgg tcgcatcagc cgtggtcacc gtttccttgg
    21481 aaatgaaact gagattgcaa gtgcagattc ttacgaggca gacttgcgtg cacagtttgt
    21541 tatcactgac cccgcagagc gtcaaaatat gatcgtcgag caaatcaagg ctatcgagga
    21601 caaacacaac gtgacagttg aaattgatga agatttgctc aacgaagttc tcaatctggt
    21661 cgagtaccca actgccttca tgggttcttt tgacactaaa tatttggaag taccagaaga
    21721 agttttggta acttccatga aaaaccacca gcgttacttt gtagtgcggg ataaggctgg
    21781 caagcttttg ccaaacttca tctccgtccg aaacggtaat gaccagtacc ttgacaatgt
    21841 cattaaagga aacgaaaaag tcttggtggc ccgtttggaa gatggtgagt tcttctggcg
    21901 tgaggaccaa aaactcaaga ttgctgactt ggtagagcga ctcaaagttg tgaccttcca
    21961 tgagaaaatc ggctcacttt acgagcacat ggaacgcacc aaagttatcg cagaaaaact
    22021 ggctgacttg gcaggtttgt ctgcgggtga aaaagcagat gtggcgcgtg cggcggacat
    22081 ctacaaattt gacctcttga caggcatggt cggtgagttt gatgaattgc aagggattat
    22141 gggtgagaag tatgcccttc ttgcagggga aaaacctgcg gtggcagcgg caatccgtga
    22201 gcactacttg ccaaactcag cagaaggcga attgcctgag agcaaggtcg gtgcggtcct
    22261 ggcactggct gataagtttg acaccctcct atccttcttc tcagttggct tgattccgtc
    22321 tggttcaaac gacccttacg ctcttcgtcg tgcaacacag ggtatcgtgc ggattttgga
    22381 agcatttggt tgggaaattc cattggatca gttgattgcg gagctctaca gcttgaattt
    22441 tgctagcttg acctatgaca accagccagc tgttatggac tttatccgtg ctcgtgtaga
    22501 gaagatgatg gacaagacca ttccgaaaga catccgcgag gctgtacttg ccagctcaac
    22561 ttttgtggtc agactgcaac tggcagccag ctctgctatt ttccaaaaag caaaagaggc
    22621 tgactacaag gaagccgtgg aaaacttgtc acgtgtcttc aacttggctg aaaaggcgga
    22681 ggtgactgcg attgacgagg ccctcttcga aaacgaccag gaaaaagccc ttgctgctgc
    22741 agtggcaggc ttggagttga cagaagatat ggcgggcaac ttggacaagc tctttgctct
    22801 cagcccagtc atcgcggcct tcttcgacaa taccatggtt atggtagatg acgcaacagt
    22861 caaagcaaac cgcttggctc tcttgaaagc cttggcggat aaggcaagtg ctgtggcggt
    22921 cttcaatctc ttgaatagca agtaggaggc atttatggaa caagcaaaaa tcgatcgcat
    22981 caatgaattg gcccgcaaga aaaaggcgga aggcttgaca gaggaggagt tgcaggaaca
    23041 agcagctctt cgtgaggagt acatcgaggg ctaccgccgt agtgtgcgtg cccacatcga
    23101 aggtatcaag gtcgtcgatg aagacggcaa tgatgtgacg ccagagaaac tccgtcaagt
    23161 ccaacgtgaa aaaggtttgc atggccgtag tttggatgat ccaaattcct aagaataaat
    23221 gataataaac aaggaaagac taggttttct agtctttttt tgtgcttgca gcctacttac
    23281 aataacgaat cactcgagat atagtcattt gaattagctt tgatacatcg tcactttcag
    23341 cttgccatac tctacgaaag tgacttgttt cgcaagtctt atttccaagc tagaacagcc
    23401 tagaggttgt tctaactacc tgcacctcag ttccttgtct aaaagctctt tatttgacta
    23461 tagcaaagcc ttgaaaaaat ctctggctca gactaaaaaa tgctacccaa cagcccgctc
    23521 taggctgatg agcaggatta ttatagcccg ctcttgacag aagttgagtt agaaagtcaa
    23581 ttgccatagt agattatttt gacttgtatt tacgataaaa tttatgttaa aatagggacg
    23641 gaaacaaata gatagggagg ttggtcatga aaaaactggt gtatgccttg ttggtgcttg
    23701 cctatgttac ggtcgcttat ttgtcggtct atgtccttcc acactattat tctggtgcaa
    23761 ttataggagc ggcaggagca agtcttggtg ctagtttcaa acagttcaag gaagtgaaag
    23821 cttctcctga taaagcctta ttggcttatt ttaagaggga tgagaaaaag gcaagtagtt
    23881 tattattggt ccttctggga atattccttt ttgttagcct tgtgttggaa tttaattggc
    23941 aatatggctt agcttttgta gtggcgatca gctatgctat gctttggaat gtccttcata
    24001 tccaattctt acgaaaatac tattttaaaa acgcatgaga gtttcatgcg ttttcttttc
    24061 taaatacttt gtcctgcttt taattttttc ataaagatga tggaacttgg aatcaggacg
    24121 atgatgcttc cgatccaggc ggcggggtta gcggcggcgg ttccataaaa accgaaatac
    24181 tgcatgccga tgatggcgac tgctgctcgg aaaatcaatt ctccaaagcc tgctagtgtt
    24241 gggataaagc ccttgccaag tccctgaata aagctacgaa gaataaagag ggaagcaaca
    24301 atccagtaac aagagccgtt aatgatgtag taaacaagag ccaagtccag cactgtttgg
    24361 ctggcatttg gcagaaagag accagagaag aagcgattgc ccaagatgag aatgaccgca
    24421 aatacaatgg cccaagcaat gctgaggagt aatgagtggc gcactccttc tcgaatacgg
    24481 tcatagagct tggcaccgta gttctgagca gtaaaggttg cgatagccaa gtccagattg
    24541 accatgggca gcatagccaa ctggtcagta cgaagggcaa tggcctgggc ggcgatggcg
    24601 tctgttccta gttggttgac cataaactgc aaggtcatgg ctccgatggc gataatactg
    24661 gcttgaaatc ccattggaaa agcaagctgg gcatgtttct tgagattacc tctatccaat
    24721 tttaaatcag ctagtccgat atgatagtga ggtaccttgt ggataatgta aaaaataaga
    24781 aagaggaccg aacaggcttg agcaaggata gttgcaaagc cagctccgaa aacgccccag
    24841 ttcataacta agataaagaa aaaatccaga ccgatattga taaaacaagc gataactaga
    24901 gccagtagag gtgttttaga gtttccgaga ctgcgtaggg ctgaagaaag aaaattatag
    24961 aaaatagtaa aggtcaatcc tccaaacatg gccgttagaa aggcatggga gtggtcgatg
    25021 atactcacag gcgtttgcat taagaccaag ataggtttca aaaaagctaa ggctgagact
    25081 gttagtaaaa ggctgatgag ggaagcgtag aagagtccgt ggacaaaaga ttttttaagc
    25141 ccctctaagt cgccagctcc aaagcgttgg gcagaaataa tggttaatcc attggtaaca
    25201 ccttgggcaa aaccgatgat taaaaaaatc aggctggctg tcgaaccgac acttgcaaag
    25261 gaatcctttc ccaaagtatg ccccacaatc atggaatcag caaaattata ggccagttga
    25321 aaaaaagagc caatcaataa gggaatagcg aattgtaaga tgactgcaat cggctttcct
    25381 ttggttaaat cattcataat ctctcctttt gacagatgat aagcccacta gataaatgat
    25441 agtctagtag gctttctagg gataaaatat ttctcctgaa aacggagtat agggcgtaaa
    25501 tttgttattt atactcttcg aaaatcaaaa tcagacgttg ttgacttgat ttgatgagtg
    25561 aaaggctcca gtggagcctt tcagcctgtt accttgaaac aaaatagtaa cagggttcta
    25621 tctgcgtaag tagtaacgaa ctacgttcgc cctatctcca acctccaaag gttccccaaa
    25681 cctttggagc tagtctgttt ttgattttca ttgagtatta atttgtcagt tcttcaaaat
    25741 gatcgacagt gttatggtct gttactgcta agatgagttc gtcagatttg aagacatagt
    25801 caggagtcag ttggatgttt aattcctgat tttcgccact tcggtaaccg atgatattga
    25861 gtttataatg ttggcggagt tttaattcgc cgagtgtttt tccaatccat tttactggcg
    25921 ggtgaaactc gacaatggaa acattgcctt cgagctcaaa gaggctggtt gtatcgcggt
    25981 ggagtagttg ttttgctaga gaaatacctg tttcacgttc aggagaaata accttatcgg
    26041 caccgacacg aagaaggact tctttcgcag ttgttccttt gactttgaca atgaccatag
    26101 gaacacctag aactttgcta tgcatgacag ccagaacact ggattccaag ttttcgccag
    26161 tagctacaac aacagcatcg cagttaccga ttcctgctgc acgtaggagc gagcgatcgg
    26221 tgatgtctcc gacaataccg cgtgtgagaa taggttctag atgattgata cgttgctcat
    26281 gattatcgat agcaatgata ttgcaatcgt aattgtgtaa tgtttttgca atggtagtac
    26341 caaaaactcc caggccgaga attccgatag tatagtttgg catgatttct ccttttaaac
    26401 aagcatagat gattttgcgt attgaagact ttcggtttta gataattttc gaacagaaag
    26461 gcttgcgaga atagaaagtg gaccgatacg gccgaagaac atgagcgcca taatgactgc
    26521 ttgaccggcc atggttaggc ttggagtcag atttgccgta acacctacag tggctaatgc
    26581 tgacatgacc tcgaacatta aaaatagaag tggttgtcgg tcatctgtta gggcaagtgc
    26641 gaagagccct atgataaaca taagtaggaa gactgagaat gtcgcaaaag ctttgcggat
    26701 gctgaggtta tcgatggttc gtcccataaa gttggtatga gggagaccga gaatctcgct
    26761 acgagcatag agcactaagg ttaggaaggc ggttatttta ataccgccgg ctgttccacc
    26821 tggggctccg ccaagcatca tctgaaagat atagagcaag agggtgattg gttcagcctt
    26881 cgtatagtcg atgctggcaa aaccagcggt tctcatggtg accgtctgga aaaagctggc
    26941 taataattta tggccaaatg atagttgtcc gatagtagaa gggttgttcc attcggtgag
    27001 taaggtagtc agagttccga gggtcagtat aatggctgtc aggcctagaa caatctttgt
    27061 atggaaccct agttttcgta ggctacgttt ttgcccgatt tgtgtctgaa ggtcaaacca
    27121 aaccgagaat ccaagacctc ccataataat gagactggct aatgttatgt tgattagagg
    27181 attatccaca tagcgcatga tgctagttgc gccaaaatta tcaaaaccag cattacaaaa
    27241 ggctgatata gctacgaaaa tcgaggtaaa taaacctctc gcccatccaa attcaggtac
    27301 aaaatgaaag gacagtaaga gggctccaaa ggcttcgacc gccaaggtaa aaccaaaagc
    27361 tgatttgagg aagttacgaa aatattttgt gtcagatcgg tttagagcct cttgtaaggt
    27421 cgccatattg atgaaattca ttttacgacc accgcgtaga gcgaagagac caataaatcc
    27481 tatcaagctc aaaccaccaa tttggattaa catcatacag ataatctgtc caaaagtaga
    27541 ataggtttca gcaacggctt tggtaaatag ccctgtcaca caaaccatgg agacagatgt
    27601 gaagagatga tcccaataac ttgctgtaga ggtgctaatt tgtgagatgg gaagacttag
    27661 taaaattgat ccagtcagaa tcacgaataa gaaactgata atgattcgct gactgggaga
    27721 aagtttgatc ctaaatggtg acataattct accttttctc aaaaaaataa aagatgaaga
    27781 tattgtagca caaaagaaag aaaatgtacg actaaaacac gcaaagaacc tgattttttt
    27841 ctcttcttga aagcttcatt attttttatt accatggatt ttataagtgg tgtttatatg
    27901 aggtggctca ttatatttgt gacaccagga ttatggggga attattatgg acaatgaaaa
    27961 agtggtgcgg accataaaaa gaatctcctt agttgaccta gggagattta tttccattct
    28021 gctaagactt gttgatgata gtgacgtaaa ggtccgacct tttggaggag gtaggtagca
    28081 gccagtccga ccaataagcc tgataaaatt cctgtaaaag cgaggattgg caggtagttc
    28141 agtacataga aagaacgggc gatggtagca aaaacaagaa gctgaccaag attgtgcaac
    28201 atcccgccca aaatagaaat tccaatagga ctgacacggt ttggaccgag ctgcttggca
    28261 gagagcattc ctacataact caaagtagtt ccggcaaagc catagaggaa ggttgaaaag
    28321 gtgcctccca gaaaggttga gatgagcaag cgtagtgcga caacctttag gctatccttt
    28381 gtagggaggg taaaaatagc aattaagcta atcaaatttc ccagtcctag cttggcgcct
    28441 ggagcaaaag caaatggggg ggtgatgagt cgttcaatca aagaaatgac aactgcctga
    28501 gctgctagca tcgtaataaa gttaagtgtt tgacgtttag aattcatggg tgtagagaaa
    28561 aaactctttc tagtcgatta tctattttac tatcatactt gtttttgttc actatggcaa
    28621 gttttgtttg tgtatattac taattattga aatttgcttg tcaactatct tggaatacga
    28681 tataatggaa acatgtaatt tgttttgatt ttcacaagga ggaaacattg tgacgaaaaa
    28741 tattgtgatt gtcggtgctg gttatgcggg gattgcagca gctcgattgc taggaaaaac
    28801 ttttaaaaag gatcaagatg taacagttac cttgattgat aagaactctt tccataccta
    28861 tatgacagag ttgcatgaag ttgcggctgg gcgtgtagaa gccaatgcaa tcaagtatga
    28921 ccttcaacgt atcttcaaaa aatatccaaa ggttcaattg gtaacagaca aggttgttga
    28981 gattgactac gataagaaac aagtagttgc ggaacaccaa actctggatt ttgattacct
    29041 gctccttgca atgggtggtg aggctaatga ctttggtgtg aaaggtgtta aagaacacgg
    29101 ttttacactt tggtctatcg aagcagcaga acgtttgcat gaccacatga ttgacgcttg
    29161 ctatcgtgcc atgcgtgagc atgatgaagc caaacgtcgc gctcttttaa cctttactgt
    29221 tatcggtgct ggcttcacag gtattgagat gattggtgag ttgattgact gggtaccaat
    29281 tttggcgcgt gagttcaagt tggattcaaa agaattctca cttaaggttg ttgaggcaac
    29341 gccaaatatc ttggctatgg ttactgaaaa agagcaagtc aaggctcgca aataccttga
    29401 gaaaaaaggt gttgaattgg ttcttggcga tggtgtagca agtgtacaag aagatagctt
    29461 gacactttca tcaggtcgtc aaattcctac ctatacctct atttggacag ctggtgttca
    29521 agccaataca gacgcaagtg aatttggtat tgaaaaagca cgtgcaggtc gtttgatagc
    29581 caatgagttc atggaagcga aaggcaagga aaatgtctat gtagctggcg acttggttta
    29641 ctttgaagaa tcagaaggaa aaccaactcc acagattgta caagcagctg aacagacagg
    29701 ccatactgcg gcaagtaata ttattgcagc aatcaagggc ggcgaaaaac atagctacaa
    29761 aggtaaatat gatgggttta tggtatctat tggagcacgc tacggtgtag ccttcttgat
    29821 ggacaaatac cacatgtcag gctttatggc aatggctgtc aaacacatgg taaacctact
    29881 ctacttcttt actatccgta gtttcttcta catgggttct tatgttcgtc atgaattctt
    29941 tgacattcag aataagcgaa acatttttgg cggtcatact tcaggcaaag gaaatcttct
    30001 ctggtctgtg ccaatgcgtg tcctttatgg ttcagtatgg ctctacgaag gtatcaagaa
    30061 agcctttggt ctatttggaa caacatcatg gtttggggat caagttgtct tcccattccc
    30121 ttggttagct gatccagtat ctggagcatc tgctgctgag gcggtttcaa gtgcctcaca
    30181 agcggcaact gctgcggcag aagtagctga accaattttt ggtttgagct atgcttatgg
    30241 tgaagcaccg atggctgttt tggacaaaat gcctgattgg tttgctacta tcatggaatt
    30301 tatgatgcca aaccaagaag tagcactctt tatgcaaaaa tttatgacag tagcagaaat
    30361 cggtatcggt ctggctttga ttgctggtgc ctttgtatgg attgtatctg ctgcaaccgt
    30421 tgctttggta gttatgttca gcctgtcagg catgttctac tgggtaaata tttggttcat
    30481 cccagcagct atttctctta tgaatggtgc gggtagagcc ttcggtctag actactggat
    30541 tatgccatgg ttgggtcgtt tcctagataa gaagatttat ggaaagccga agcatattta
    30601 ccgtatcaag gataaaaaat aaaggaaata tagaaagtta gtggtacatc aaatttggaa
    30661 tacctatcct gagattgaga ggggacttga agaggtaaaa tccatcatct tatctgagat
    30721 gatggtcctg catccagcag ttacagcgaa gattgttgaa tatgtagagg cgccagggaa
    30781 atacctgcgt gccggcctct gtctgctctt tgcacaaatg acggaaggaa agattagccg
    30841 ttcaaagcta tattttgctg cccatttaga agtcctgcat ctggcgactc ttattcatga
    30901 cgatgtgatt gatggcgcgg ataaacggcg gggagtcacg gcggctcatc aacaattttc
    30961 gaatcgaatc gctatctaca cgggtgatta tcttctggcc tattcaagtc gactatttgg
    31021 aaaggggctt cgtttgctag aggtgtcacg tgatgatttg gatttgggca atgataagct
    31081 gatggaaaca attcttcgag gggaattatc tcagctactc aatcaatttg atgccaatat
    31141 gactatgaaa gcctatctca agcaaattca aggaaaaaca gcttttctat ttggacttgc
    31201 ctgtcagttg gggagttttg taccaggcca atctaggaaa gacagtagca tggcttttcg
    31261 gtctgggcaa gcacttggca tggcctttca gttacgagat gacttaattg actaccagct
    31321 ggatgtggca gaatcaggaa aacctagatt gcaagatatt caaaacggta tctatactgc
    31381 accgctactt tttgccatgc gagaagacaa gtctatccag cagcaattgc gggcttatat
    31441 tcaatcccct acggcagaag ggttggaaat ccttacagat aggatttttg caacgcatgc
    31501 tattgccaaa acagagaaac tgattgttag ctatttggaa aaaatgatga gttttgtaga
    31561 tagaatatca cagttatcga ctgcgtctat gcaaaatttg gtggcaatag tttttagtca
    31621 ttatttttaa aaaaatacta gacaatttag tttattcggt atatcattat aagtataaaa
    31681 ctatttactt tacaaaagga gtattgttat gaaaacaact aaagttgtgt tgaaaagttt
    31741 ggtagttctt ggtgcggtat ttgcacttgt tgcttgtggt tctaaacaag aatctacttc
    31801 aacttcatct tcaactacag aaacagtagt attgaaagat ggtacttaca aagctgagtc
    31861 aggcaaagac gattatggct acaaaattgt tcacacattg actgttaaag acggaaaaat
    31921 cagtgagtca aaatttgact acgaagctga agatggaagt cttaaatcag caaatgaaga
    31981 gtacaacaaa aacatgaaag ataaagctgg agtttctgct ggtgaagcaa tcgaacaact
    32041 caatgcagct ttggttgaga aacaagacct ctcagctgtt gaagttgtat caggtgcaac
    32101 tcatacttct gaagacttca agaagagtac agaagccctt cttgcagcag ctgctgaagg
    32161 aaaaacagaa actgtaaaac ttgactaatt aagtctatac agaaaagaca cagtcgcact
    32221 tgtttggcta ctgtgtcttc ttatttttgg gggaaggtaa aatcagtgaa aaaaattgca
    32281 agtctattgt tcgtgggcct actgatggtt ttgcttgttg cctgtggtgg taaacaaaac
    32341 gaaagtttat ctgttgtgaa aactccgttg actcgtagtg aaagcttact tcacacagta
    32401 gtgcagttga gtatttatca tgaaaatcag gaagaagcga tggatgaagc tgtctcctat
    32461 atcaaggaga tggaaagtct gttgtccaca aacttggaag gggcagatgt ctaccgtatc
    32521 aatcaggcag caggaaaaga agctgtcaag gttgatgaac gtacctttga agtgattgaa
    32581 acagctattg acatgagtaa agaaagtcag gggctatttg acatttccat tggagctgtg
    32641 agcaatctgt ggaaaattgg agatgaggat gcacgtaagc cgagtgatga agaaatcaaa
    32701 gctgctctgc catttatcaa ttataaagac attactttag ataaggagaa gaagactgtc
    32761 tttatcaaag aaggaatgac cttagaatta ggagccattt ccaaaggtta tattgcagat
    32821 aaggttagag aattgttcgc aagcaaaggg attacgacag ccatcattaa tctaggtggg
    32881 aatgtagtgg ttatggggga ttcgccaacg acacaaaatg gttggaatgt gggagttcaa
    32941 gacccagacc aagtgcgtgg atcaacggtc ggttctgtgc cagggacaca tgattcggtt
    33001 gttacctcag gtatttatga gcgctactta gaggtggacg gtgtcaaata tcaccatatc
    33061 cttgatccca aaactggcta tcctgtggaa aacgatatct caggtgtgac agtcttttcc
    33121 aaaacatcta tccaaggaga tgccttctcc acaactctct tcttactagg tgtggaaaaa
    33181 ggtcttgact ttatcaatca gattgacggt gtagaagctg tttttatcga taaggagcag
    33241 ggcgttcatc tgagtgatgg cttgaaagaa agctttgaat taacgaatga ggagtatcat
    33301 cttgttaatg aataaatcaa ctaaagggct cagtttgtct gtatttctag aatttgttga
    33361 aatgaagacc aaggtagcta gtgtttttcc gatgaccctg gggattttat ggagcctgta
    33421 tcgctatcaa gctttcaatt ggctcaatac tctcctcttc attattgccg tccttagctt
    33481 tgacatgtgt acaactgcca tcaacaatag catggactac cacaaggcca aggatgaagc
    33541 ctatcgtcag gaaagcaatg tgattggaaa attctcccta gattttcgtc agatgattgg
    33601 aatcgtcctt gctctactta ttttttctct actggtttca ttactccttg tatggcggac
    33661 cagttggctc ctcctaccga tgggagctct ctgtttcctc attggtatct tctatacctt
    33721 tggacctatt ccgctttcga gaatgccact gggtgaagtt ttctctggag taaccatggg
    33781 atttggcatc ttctttcttg ctgtatttat tcagcaacca gatttgctac tgaccagcca
    33841 agtgagcggt cagtggatga ctttgcaatt ttcttggata aaaataattg aaattatttt
    33901 catgtccatt cctctggtaa ccttgattgc taatatcatg ttggctaaca acacctgtga
    33961 tttagaagaa gatattcgca accatcgtta tactctggtt tactatattg gcaagaaaaa
    34021 tgctcttaaa ttgtattttg tcttggcaag tttaccatgg ttgctgtgga taatctactg
    34081 tctgatagga ttcttaccaa tttgggcatt ggccggttta atcggagttt ggcctgctta
    34141 taagagtcta gaaacatttt tgaaaaagca actcaaacgg gaaaccttta tcgaggcagt
    34201 aaagagtttt gtactctttg cgctgctata tgtggtgata cttgctttag cccttatttt
    34261 ctaaaaagga aagacacagt cgttcatcag gctgtgtctt ctttgttata tcgtcattta
    34321 gtttttcttt tattacttcg acgagcgagg aaacatcgtt tcctaatttc caacttcaaa
    34381 cactccactg gagtatttga actcgccttg tctgattgaa acagtctggg ggactgtttc
    34441 aagtcaatgc ctagaaataa gaaagcattg agttatgcct gcggcttttg atgcgaactt
    34501 tgttcgcttt atctccaacc tccaaagatt tcccaaacct ttggagctag tactaaaagt
    34561 aaactaaaag actatagtgg taaaataagt tcttcttcgt cagtgttttc cccgccaacg
    34621 gatttgattt caatcaacag gttatgtggc aggcagacgg agagttgacc tggctggcta
    34681 atccagctgg tcataacggc gatttggtct gggctattgt cttccttgac acgaatgcgt
    34741 tcgccatcaa cttcaatgat attgtattgc ccttcattgg gatagtaggt cttttcttga
    34801 cggggtgtgt cctttgataa ttcaaattgg tcgaccacct caccattgat gcggacatag
    34861 gcaattgttt ttgcctcatt tgcatctgtt gtcagttgtg tgtaggtaaa aattgctgga
    34921 agaaaggata aaactagggt gaatccgatt aagatataat caaatagttt gagttgtttg
    34981 agaatggatt tgaatgtcat atacaatcta ttttatagaa aaaatcctac tttgtaaaat
    35041 gttggataca gactactttt tcaattgtcc aataaagagc aggagagcaa atattcccca
    35101 aaatccagct aggagtggtt tgatttgacc agtgaaaaat ccaatggaga aggttaggac
    35161 aagacctgca aagagaaaac atagcagtgg tttacgataa agatggatag cttcttggac
    35221 agcatttgat tctggactgg tagtttgcca gtcactattc aagattggaa gtatagtgaa
    35281 cggtaagata aagattggtg gattgtcccc agtcaggtag ctatgtatat acaggctaca
    35341 aaatgttgta taggagagaa caatcaaagt gagtataagt ttcggtttgt taacgttcat
    35401 ctgttttctc ctttttgaaa tacaatcgat aaagaatagc cagagcaaat ccagcaggaa
    35461 ggatgaggat atgtggtttg acatgtccta gccaaatgcc cacggaatag gtcaaggtca
    35521 tggcaaacag ggcaaagatg gcttgaatga taaagtatcg tttttcttct tctgttttaa
    35581 aggatctgat aatctgaatg ggcggtaaca tgagcaagaa tgaaccgcta atcggctcat
    35641 tgaccatgta agagtagatg aagaatgcag aaaataaagc ataaaataga acaaaaaatc
    35701 gacaggtttg tttgggtgta aaaggtttat tcattaggtc tgctccttat tttttcttgg
    35761 cagtatctgc ttccagtttt tgcagatagg caagggcaga cagggctaga ctggcaaaga
    35821 ggcccatcat accaccgaag aggagggatt tgacgtggaa gctggaggcc agtaggggaa
    35881 gctggaggct aaagaggtct gacagcccag caaagaggct agatagcagg gcaacaccca
    35941 gtccccaaac gagtggattg agggtctgtc cttgtctttc ctcaggactt agttcctcca
    36001 tgtcatattg aggcaggtca gcatggaggg tgcgcatatt aacgatacca attaggataa
    36061 tactgaagaa ggcaattatc attggatagt aggcagccac ctccacagga tagcggtagg
    36121 ctaggaagta ggcgatggca tttccaagag ctaggaagta gaagtagtag ataaaggcct
    36181 gtccgccgat agcatcagcc tcggcctgtc ggtattcgtc taaaggaccg cggataccat
    36241 aaaaacgttt aatcatattt ttgatccact tgggttcttc ttttgaaata gcagagctga
    36301 tagttaggtt ttctgccttt ttcatgcgct tgcgtaacat gatttcaaga gcaatgcccg
    36361 tgaaaatacc acctagacag gctgcaagga attttcggag gtcaaatagg tggtcgaaag
    36421 ggttgagccc ctccatccag gcaccaaaaa caacagtcca aatgtacata acggcagtga
    36481 aatagagggc gaatttgata cttgctccct tgagctgctt ctgttccttg gtagtcagtt
    36541 cctctacatc taaactgtcc acttggctgt ggcgcatctt ggatgtcata tacatactgg
    36601 aggaaagagt ggataggagt agagcagttg ggtagccata ggcaacgact tctgggtagc
    36661 gtttggatag agcgagtgcc agtagggtaa gagccatggt gccccaaaag agcaggataa
    36721 agcaggtatt gcctagtcgg gaggcttctt tttggcgaaa ttcatccaag ggtcctgcga
    36781 taccgtaaaa tcgtttgatt aatttatctt gaaaggtttc ttttttcata agcagctcct
    36841 tatttttgaa cgaacaatgg taagatgtaa acaaagccta agaagtagat ggtcccagca
    36901 atcagcaatt gtagccaaaa ttttgcttgg ctgatggtgt gcaagatttc tgctccgcta
    36961 ttgccccaaa gctgaaagag gacaaagagc gggataaaaa taagggtaga gataagagct
    37021 tgacgtatat tctttttcat ggtttttctt ccttttattt gtttgcgaga attttctttt
    37081 gtaactgctt cttacaatag tagtaatgga tgaaggtgat agctcctgca acaattaacc
    37141 gacctagtag gagggtcggg tctgttagtt cttcaagaaa tacagaccat tctttataga
    37201 aagcaggggc agtcgtggaa gccaggaagg ctccgaaaaa tgtgctacga ctacttttta
    37261 caagagattt attgagcttg gtcacattaa tttcatcctc atagctttga ctaatgccat
    37321 ttttcaaggc aaggtgggaa gctaaggctt gcgtgaaacc gatgattagt atattggtta
    37381 gggggagcag aaagccaact ttttcaggtt gtcgtagggc aataagaagt gcaatcgtat
    37441 ttcctattag caggatagga aaaacaatca tgacaatggt gttactgagg cgatagcttt
    37501 cttgcagacg catttcatcc atgggaccac taatggaata aaaatccttg gttgcttgat
    37561 tgagaatttt tgtaaaaata tctttcatga tgaactcctt attgattcca aaagagacta
    37621 ttaaggtcag tctgtaaagc tttggctagg ttgatacaga ggtctagcga ggggttgtac
    37681 ttgttgtttt caatcatatt gatggtctgc ctggatacgc cgatagcctt ggccagatcc
    37741 agctgggaaa tcccaagcaa taggcgataa tctttgacac ggttcatgtg ccctcctttc
    37801 tatgtcaatt atatctgaca tacttggcta aaaatgagag gataggttga ctatcctttt
    37861 aaattgttgt caattatatc cgacatacaa gtgtaaaaaa atgtgggaga cctccctgtt
    37921 gatgtcaact atatgtgaca tgtttcttta ctgttagtat atacctgttc atataagttg
    37981 tcaactatat ttgacagttt ttttgtaaaa agtttgcaac aactttttat tttaatttgt
    38041 ggtacaatgg aaaagatatg accaaggaat ttaatcacac aactgttctc ttgcatgaga
    38101 cagtcgatat gttagacatc aaacccaatg gaatttatgt agatgcaacg ctgggtggag
    38161 cgggtcatag tgagtacctg ctcagtcagt tgacggatgg tgggcatctt tatgcctttg
    38221 atcaggatca gactgctatt gatcatgccc atattcgctt ggcatcctac attgaaaagg
    38281 gacaagtgac ctttatcagg gataatttcc gtaatctcaa gactcgcttg gcggagctag
    38341 gtgtaacaga gattgatggg atttgttatg atttaggagt atctagtcca cagttggatg
    38401 agcgggagcg tggcttttct tacaaacaag atgctccgct tgatatgcgg atgaatcgtg
    38461 agggacattt gacggcttat gatgtggtca ataactatga ttatcacgac ttggtacgga
    38521 ttttctttaa atacggtgag gataaatttt ccaagcagat tgctcgtaag attgaacagg
    38581 cgcgtgcggt caagccgatt gaaacaacga ctgagttggc agagctgatc aaatctgcca
    38641 agccagccaa ggaacttaag aagaagggcc atcctgccaa gcagattttc caagcgattc
    38701 ggattgaagt caacgatgag ttgggggcag cagacgaatc tatccagcag gccattgact
    38761 tattggcttt ggatggacgg atttctgtta ttactttcca ttctttagaa gatcgtttga
    38821 ccaaacaact cttcaaagaa gcaagtacga ttgatgttcc aaaaggtttg ccatttattc
    38881 cagatgactt gaaggcgcca cttgaattgg tcaatcgtaa gcctatctta cctagtcaag
    38941 aagaattaga ggcaaataat cgcgcgcatt cagccaaact gcgtgtagct aagaaagtac
    39001 ataaatagga gagagtctat gttgcaagaa aaacgtagag aagccactat gcgggttatc
    39061 ggtgataaaa ttagaacctt cacacgtgta gaaaaagcct tctattcaag catcgtatta
    39121 tctggattag cccttgcgat aggaattatc tttatgcaaa cgcggctttt gcaacttcaa
    39181 tctagcatgg ccaaagtcaa tcaagacatt agccaaaaac agattgaaat taacgatgct
    39241 aagcaagctg tcaatgaatt gacacgttca gctcgcttga tggaaattgc tgaaaaagct
    39301 ggattaactt ttaataatga caatattgga gtagccgaat aatgcccaga agaaataata
    39361 aactcttgcg gtatgtcttg aaaaaacgat atgttccgtc aaaaaatcgt cgaagagtag
    39421 gacagagcct acttcttttg acggttttcg tcttttttct ctttttaatc aactttgcct
    39481 atattatcgg caccgatagt aaatttgggg tggacttgtc agagagagct gatgcggtac
    39541 accagcgtga agttatcgtt caagctaaac gggggacgat atacgaccgt acaggaattc
    39601 ctattgcgga agattcaacg acctatacag tctatgccat cattgacaca gaatatgtgt
    39661 cagaactgaa aaaagttttg tatgtgcagt cttctcagtt cagcaaagta gcagaaatct
    39721 ttaaaaagca tctcggaatg gagacagatt atgttatgga gcagctgaat caacctgaac
    39781 tgaagcaggt atactttggg accttaggta agaatatctc ctatagtaca atgtcgtcaa
    39841 tccaagaaga gatgaaggcg gcaggtattg aagggattgc ctttaacgct agtccaggac
    39901 ggatgtatcc gaatggaaat tttgcttcaa tttttgtcgg gttagctaat ctggttgaaa
    39961 acaaggatgg tagtcatagt ttacagggtg tgagtggttt ggaatattat ctgaatgata
    40021 ttctggctgg tcaagacgga aaggttatct acgagaaaga tagtcaaggc cgtgtcctac
    40081 ctgggacaga aaaggttgaa acggagacag taaacgggca ggatgtctat acgacaattt
    40141 cagccgatct acaacgttct cttgaaacca atatgaatac cttttttgat aacgccaaag
    40201 ggcgatatgc gaatgcaact ttggtttcag ccaagacggg tgaaatactg gcgacgaccc
    40261 aaaggccaag ctatgattca gataccaaag aaggtctggg acaagaagga ctgctagagc
    40321 ggtcactctt atatcaagag gcctttgagc ctggttcaac aatgaaggtg ctaacagtag
    40381 cgtcagctat cgataacggc acctttgacc caaatgcata ttataccaat aatgagtacg
    40441 ttattgcaga cgcgaaaatc aatgactgga ccctcaatgc aggttattca gctgtgactc
    40501 tcaattatgc gcaaggcttc tccctatcca gtaacgttgg gatgacctta ttgcagcaac
    40561 agatgggaga tgaaaaatgg ctaaattacc tcactaagtt ccgttttggc tacccaacgc
    40621 gttttggtat gggggacgag gcgccaggaa tggtaccaga ggataacatt gtttctatcg
    40681 ccatgtcatc gtttggtcag gggatttctg caaaccaagc acagatgctt cgtagtttta
    40741 ccgccattgc gaacaatggt attatgctag agcctaaatt tatctcagct ctttatgatc
    40801 ctaatacaga tacagctaga gtttcttcga aagaagaagt aggaaatcca gtttctgaac
    40861 aagctgccga cgatacactt cgctatatga ttaatgtcgg tactgaccct gtttatggta
    40921 cgctctactc acatgactta caggggcctg caattcaggt tgacggctat gatattgcag
    40981 tcaaatcagg tacggcggaa attgccagcg aagatggtca agggtatatt aaaggtgcct
    41041 acatcaactc tgtagtagcc atgatccctg ctgaagaccc tgaatttatc atgtatgtga
    41101 ccattcgcca accggaagta ttaaatgtcc tgtcttggcg tgacattgtc agtcctacat
    41161 tgaaagaagc gatgctgttg aaagacaatt tgaatctgga ttcacctgca ccagcattgg
    41221 cacgggtcgc taatgagaca gagtatcaat tgccagacct cattggtaag gcaccaggcg
    41281 agtcggctga ggaacttcgc cgtcaattag ttcaaccagt tatccttgga aacggtgctg
    41341 aaattaaaaa agtttcagtc gaaaaaggaa gtaaggtaat cgccaaccaa caggttctac
    41401 tcctgacaaa taaattcgaa gaaatgccag acttgtacgc aatcaccaaa gagaatatgg
    41461 atatatttgc tgaatggact ggtatcgaag tgacctataa aggaacgggt tcaaaagtag
    41521 tcgaccaaag tgtagaggtc ggtaccgctt tgaataaaac caagaaaata acaataacct
    41581 taggagactg acatgcaatt cgcacttatg tcgggattgg tagcttttct agcaaccgtt
    41641 ctcttaatcc cacgatttat tacattttat caagccaaac gcattgaggg gcaacagatg
    41701 cacgaagatg tgaaacaaca tcagtttaag gcaggtacac caacaatggg gggaacagtt
    41761 tttctcgttg ttgcgattct tgtcagcttg ttgtttgcaa cggcctttca actgttgacg
    41821 ggtggtgtcc ttgccatcct cttcatcctt gccctttatg gtgtagtagg atttttggat
    41881 gactttttaa aaattttccg taaaataaat gaaggtctta atcctaaaca aaagcttgct
    41941 ctacaaattt taggaggtat tgtcttctac tttgtccacg taagaggagc aggtggcgga
    42001 gaattgaatg tatttggtca catggtacat ttgggagttc tctatttccc atttgttctg
    42061 ttctggctgg tcggtttttc aaatgcagtt aatttgacag atggcattga tggattggct
    42121 tctatctcag ttgttatcag ccttctagct tactctgtca tcgccttcaa tgagcaaaaa
    42181 tttgatattc ttctagtttg tgtgaccatg attggtggct tgctcggttt ctttgtttat
    42241 aaccgtaaac cagcgaagat tttcatgggg gatgtgggaa gcttggctct cggcggtatg
    42301 ttggcaacta tttcaattgc cctccgtcag gaatggactc tcctccttat cggcctggtc
    42361 tatgtcattg aaacgtcctc tgttatgttg caggtttcat attttaaata caccaaaaaa
    42421 cgctttggag aaggtcgtcg tatcttccgt atgactcctt ttcatcacca tttggagctt
    42481 ggaggtttga ccggaaaagc tgaaaaatgg agcgagtgga aagttgactt tttcctctgg
    42541 tcagtcggtc tcatcatgag cttgattacc ctagctattc tttatctata aaatgtcaga
    42601 aacagcagaa atgctgtttt tttaccttga caaatatggt cggggggggg gtaaaataga
    42661 gagagaattg gtggaggata aaaatggaac aattcaatca agaacagtta tatggagtgg
    42721 ttattcaaac catgacagct gctagtcaac ttcctatggt taaagtcaat cgggaagaat
    42781 ttttgaagac tcgttttaaa gatagtccct atctagataa aattattgcg gatggaccaa
    42841 cggcggtctt cactacaaaa gctttgcgta aggaagctga aaaaatcatt aatgatatga
    42901 ctacgaaaac atctttgatt tctttcgcag ctggtttacc ggggaatcca tttacagcgg
    42961 tggcggcagg tactgctgat atagctcagt attttggttt tgccttgaat ttgtcgcaac
    43021 aaatcgctta tctttttggt gaagatgaat tatttgttgg tgaagggaag gaaatttcag
    43081 aagaggttca aatccgcatt attgcctaca ttggtgtcat gtttggagca ggtggttcag
    43141 cagttcttct caataaagta tccaagacag tcggagctaa tatcggtaaa aaagtagcta
    43201 tgcaggcttt gaccaagaca acttggtatc ccttggtgaa aaaggtagcc gcagtgattg
    43261 gtgtgaaaat taccaaaaag actgttgaaa agaccattgc caatgcagtt cctattttgg
    43321 gtggtctgat ttcaggaggt ttgacctatt ttaccttcca gcccatggga gggatgttgg
    43381 cagacacatt tgtgaaaaaa ataaatggtg attttgatgg cgaattagaa ctcactgagg
    43441 cctttaaagc aagtttggaa gaagagaagg aacttggaat tattgaggca gaatttactg
    43501 aagcaagtga gagttgatta ggacgggtat ccgtcctttt tgcttgtcca cctaaatcgt
    43561 gctataatag agggaataca actaaagagg aaactatgaa atttacagat atgaaactca
    43621 agccttatat tcaggaggct ttgaaagaaa ttaactttgt ccagccgaca gaagttcagg
    43681 agaaattgat tcctgttgtt ttgtcagggc gagacttgat aggggaatca aaaacaggtt
    43741 caggtaagac ccataccttc ttgatcccga tttttcaaaa attaaatgag gaattggacc
    43801 aagttcaggc tgttattaca gccccatctc gtgaactggc tactcagatt tatcaagcgg
    43861 cacgccagtt ggctagtcat tcggacacag aagttcgtgt gaccaattat gttggcggta
    43921 cggataagaa ccgtcaaatt gacaagcttc aagctagtca gcctcacatt gttgttggga
    43981 caccagggcg tatctatgat ttggtcaaat caggtgacct tgctatccat aaagccaaaa
    44041 cctttgtggt tgatgaggca gatatgacct tggacatggg ctttttggaa acagttgata
    44101 agattgctgg cagcctgcca aaagacttgc aatttatggt cttttcagcg accattccgc
    44161 agaaactcca accgtttttg aaaaaatact tgtccaatcc agtcatggaa caaatcaaga
    44221 cccatacggt catttcggat accattgaaa actggctgat ttcaaccaag gggcgtgacc
    44281 gtaatgctca aatcttggaa gtaaccaagc tcatgcaacc ttacttggcc atgatttttg
    44341 tcaatacaaa gacacgtgca gatgaattgc atagctattt gactgcgaac ggtcttcgtg
    44401 tcgcaaagat tcatggggat attcctccac gtgagcgcaa gcggatgatg aaccaggtga
    44461 agaacttgga ctatcagtac attgttgcaa cggatttagc agcacgtggg attgatattg
    44521 agggcgtcag tcatgtcatc aatgatgcca ttccacagga tctttccttc tttgttcacc
    44581 gtgtggggcg cacaggtcgt aatggtttat caggcattgc cattaccctt tatcagccaa
    44641 gcgatgatgc agatattcga gagctagaaa aactcaatat caaattcttg ccaaaagaaa
    44701 tgaaaaatgg cgaatttgtc gatacctacg accgtgaccg ccgtgctaac cgtgaaaaat
    44761 ctcgtgaaaa actagatttg gaaatgattg gcttggtcaa gaagaaaaag aaaaaagtca
    44821 aaccaggcta caagaagaaa attcaatggg ctgttgatga aaaacgtcgc aagaccaagc
    44881 gagttgaagc gcgtgcaaag ggtcgtgcag aacgtaaagc caagcgtcaa accttttaaa
    44941 gtaatcgagg aaaaagatgt tacaaaacat tctaagtttt tatttacaag gattgctaat
    45001 agcttccttg ttgattatca cttctagtct ggtctggttt atctggcgtg cgacaaaggg
    45061 tgtggataaa accttgcaag aacgacaaga tttcttgttt gatttgttga tgattaatgt
    45121 gatgacaatt cccattgcag cctttggagt agtgggaatc ttattaatgt ttaaagcgta
    45181 gaaggaaaaa gtatatgtac gatacagttg taattggtgc gggtcctgcg gggatgactg
    45241 cagctcttta tgcagggcgt agcaatttaa aggtagctct tttggaacgt ggtatttatg
    45301 gtggtcagat gaacaatacc gcggaaattg aaaactatcc aggctatgac catatttcag
    45361 gtccagcctt ggctgaaaaa atgtttgaac ccttggaaaa attcggtgtg gaccatattt
    45421 ttggcaccct agtccgtatt gaggaagaag gacaaattaa gaaagtgatt actgaggatg
    45481 gtgttttgga gacaaagaca gtcgttctag ccatgggggc aaaacaccgt ttgttaggta
    45541 ttccaggtga ggatacttac aatagccgtg gtgtctcata ttgtgcagtc tgtgacggtg
    45601 ccttcttccg tggacaaaaa ctcttggttg tcggtggtgg agattctgcg gtcgaagaag
    45661 ctctcttctt aacacagttt gcggagtctg tgacaattgt tcaccgccgt gatcagcttc
    45721 gtgctcaaaa agtcatccaa gaccgtgctt ttgccaatga aaaaattaac ttcatctggg
    45781 atagcgtagt cgaagaaatc aagggcgatg acttacgtgt acaaagcgtg gttatcaaaa
    45841 atgtgaaaac agaggaagtc agcgaactgg acttcggtgg tgtctttatc tatgtcggtc
    45901 tggatccgat gactgataca gttgcggacc taggaattac agacgaagct ggttgggtta
    45961 tcactaatga aaagatggaa acgacccaat caggcattta tgccatcggt gatattcgtc
    46021 aaaatcaact tcgtcagatt gcaacggcag taggaaatgg tgcagttgct ggacaagaag
    46081 tctataacta catcacagaa ctagctgaat aggggaatac aatgtttcat atttacgata
    46141 ttcagaaaaa tctagatggc atcttctttg ataaaacctt ggatttacag gaagaattgc
    46201 aagctcgaaa tggtgaagtt ctaggcttgt cgccagtcca agtcactgga aatgtccgtt
    46261 ttgaatcagg cttctttttc ttggactatc agatgactta tgatattacc ttggcatcca
    46321 gtcgttctct gcaaccagtt gttttgcatg aggtccaaaa cgtcaatgaa ctctttgttg
    46381 ccaatgaagc agttcttaaa gaacaagatt tgattgatga ggatatggta ctggtagtag
    46441 aagatgacca cattgtcctt gatgagagtg tagcagataa tattttactg gctataccaa
    46501 tcaaagtctt gacacctgaa gaagaagctg gacaggaact accatctggt aaagcttggg
    46561 ctttaatgac tgaagaagat tttcaacaaa aagctcaaga gaaaaaagaa gccaatagtc
    46621 cttttgccca attacaaggg ttatttggag atgaggacta actcagattg gcaagatagt
    46681 taaattatct gaaaattctt gtcaatttta agctttcagt gtataataaa agagatacaa
    46741 attagaggag taaacaaatg actaaagcaa attttggtgt tgtagggatg gccgttatgg
    46801 gccgcaacct tgcgcttaac gttgaatcac gtggctattc agtagctatt tacaaccgct
    46861 ctgctgataa aacagaagat gttgttgcaa gcaacccagg taaaaacttg gtaccaagtt
    46921 acgatgtgga aagctttgtg gcatctatcg aaaaaccacg tcgcatcatg cttatggttc
    46981 aagctggtcc tggtacagat gcaaccattc aagcgctctt gccgcacttg gatgaaggtg
    47041 acatcttgat cgatggtggt aatactttct acgaagacac tatccgtcgt tcaaaagaat
    47101 tggcaaactc aggtattaac ttcatcggta caggtgtatc tggtggtgaa aaaggagccc
    47161 ttgaaggtcc atctatcatg cctggtggtc aaaaagaagc ttacgaattg gtggcagatg
    47221 tcttggaaga aatttcagca aaagcaccag aagatggagc accatgtgtg acttatatcg
    47281 gtccagatgg agctggtcac tacgtaaaaa tggtacacaa cggtatcgag tatggtgaca
    47341 tgcaattgat tgcggagtca tatgatttga tgcagcactt gcttggcttg tcagtggatg
    47401 aaatggctga catcttcact gactggaacc aaggcgaatt ggatagttac ttgattgaaa
    47461 tcacagcgga catcttgaaa cgcaaggatg accaaggtca agatggacca attgttaact
    47521 acatcatgga cgctgctggt aacaagggta ctggtaaatg gactagccaa tcgtcacttg
    47581 acttgggtgt gccattgtca ttgattacag aatccgtctt tgctcgttat atctcaacct
    47641 acaaagatga gcgtgtggca gcaagcaagg tccttccaaa accagctccg tttgcatacg
    47701 aaggcgacaa ggctgaattg gtagaaaaaa tccgtcaagc attgtacttc tcaaaaatca
    47761 tgtcttatgc acaaggtttt gcacagttgc gcgtagcatc aaaagaaaac aactggaact
    47821 tgccatttgg tgaaatcgca aaaatctggc gtgcaggttg tatcatccgc gctcgcttct
    47881 tgcaaaagat tacggatgcc tacggacgtg atgaagactt ggcaaacttg ctcttggatg
    47941 agtacttcct tgatgtaaca gctaagtatc aacaggcagt tcgcgatgtt gttgccttgg
    48001 cagttcaagc aggagttcct gtaccaacat tctcagcagc tattacctac ttcgatagct
    48061 accgtgctga aaacttgcca gcgaacttga ttcaagcaca acgtgactac tttggtgctc
    48121 acacttataa ccgtaaagat aaagaaggaa tcttccatta cgactggtat agcgaatcaa
    48181 aatagaaaag atgggcgtgg aagcgccctt ctttctagtc ataatacgat tcctacacca
    48241 tttgaaagaa tagaaaagga tggacaatgg ctaagaaaat tttgattgct ggtaaagaac
    48301 gcaatctctc gcattttgtt tccatggaat tgcaaaaaaa agactatctt gttgattatg
    48361 catcgacagg gaaagaggct atgtccttgg cacatgaaac ggattttgac ttgattctga
    48421 tgagttttca gctttcagac atgtccagca aggaattggc tacagaactg ttagcgataa
    48481 aaccagccac ggtcatgatt gtggtagttg agccgacaga agttagtcaa tatggggaag
    48541 aagtcctctc ttatgctgtt tcctacgtgg tgaagccctt tgtcattagt gatttagtgg
    48601 agcaaatctc tgcgattttt cgtggtcgtg actttattga caataactgt aaacaggttc
    48661 acatgcatgc ggcctatcgt gatttgaagg ttgattttca aaatcgaaca gtgactcgag
    48721 gtgatgaatt gattaatttg acccgccgag agtatgatct gctggcaacc ttaatgaata
    48781 gcccagagcc tgttagtcga gaacaacttc ttgagcgtgt ttggaaatat gaagcggctt
    48841 ctgagaccaa tgtcgtcgat gtatatattc gctatctacg tggtaaactg gatttgccac
    48901 atcaagattc atatataaaa acagttcgtg gtgtcggata tgccatgcgc gattgattta
    48961 gagtagagag gagggaagct ttccctcttt ctttttctat aagcgtgtga taaaataata
    49021 gccaataagt aagaatggaa aagttatgaa tacatcattt aaagaaaaag tcatattgat
    49081 actcttatct ggagcgacat tattagctgt tctcaactgg tctagtattt atggggctat
    49141 tcaacaatta ttgggcagta tgaattcgct ttttattgga gctatttttg cctttattct
    49201 caatgttccc atgaaaaaat tagaagatca gtttgaaaaa atttcttttt taaagaaatc
    49261 caagcggtct ttggcaattg ttggagtatt aattggattt gctttaattg tgacaggact
    49321 ggtagtgatt gttcttccta cattggtgac gactgtttca gaactggttt cggttagcag
    49381 cacagccatt ccaaaatcgg taaatgccct gactaatttt cttgaatcaa atggtctttt
    49441 gtcgggacaa atcggagacc agattgccag ctttttggat cagttgaaga atttaacact
    49501 tatttcaagt ttggtcacac cgattttatc tggcttggtg tccaatgtga cggggatttt
    49561 ttcgaacact atgacgttga taatggcttt cttcttcact ctagcgattt tagggagcaa
    49621 ggaacacttg caggcgatga cgagaaagtt cttgcaggct accttgccag agaaggctgt
    49681 cagagtagtg aactatattg gagaagttat tgttgatacc tatgaccgat tcttgatgag
    49741 tcagattgtt gaggcatgta tcattgggac acttgtcttt gttagttatt cgctctcagg
    49801 aattccttat gccagcatgg ctggtatttt ggctggggtc ctatcatttg taccttatat
    49861 cggtcccttt tcagcgtgtt tgattagtgc tctctttgtg gcggtacaaa atccgctctt
    49921 ggcactctgg tctatcgccc ttttccagat tattcagctg atagaaggaa atgtgattta
    49981 cccgcgcgtg gttggtcaat cagttggtct tccgaccctc tttaccctgg ctgcagccct
    50041 gattggtggt aatttgtttg gcctacttgg tatggtcttc tttaccccaa tttttgcggt
    50101 tgtctatcgc ttggttcgtg aatgggttgc cagtcgattg aagaagcaag atgaattatt
    50161 ggtagaaagt gagtaattct cgctttcttt tatttttgtc atttttatgc tatactgaag
    50221 tgataaagtt tatgtaggag aaagagtatg cgttgtccaa aatgtcagag tttgaaatca
    50281 agtgtcattg atagtcgtca ggctgaagat ggcaatacca tccgtcgtcg tcgttcctgc
    50341 gaccaatgtg gtcaacgttt taccacctat gaacggattg aagagaaaac tctagtcgtt
    50401 gtgaaaaaag acggaactag agagcagttt tcacgtgaaa agattttcaa tgggattatt
    50461 cgttctgctc aaaagcgccc tgtgtcaacc gatgatattg atgaagtggt gaatcggatt
    50521 gaacagaagg ttcgagcaca aggtgatagt gaaatagaat ccgatgtcat tgggaatttg
    50581 gtaatggaag aattggtcga attggatgaa atcacttatg tgcgttttgc atcggtttat
    50641 cgttctttca aagatgttgg agagctagaa aatttgctta agcagatgat ctccaaagga
    50701 agtaaggtaa aaccgggaaa aaaagatgaa accaaatgat ttatttgcct atataaaaat
    50761 cagcccgttt acacctgata ttgtcagtct tagcaaatgc taccagccga ttatcggttt
    50821 tgatgcatta gcgctttatt attactttta tagttttgcc gatcaagggc aaggacgtta
    50881 caaatggact gtcattctca atcacatgga ttttgggatg aatcgtttgg agaaggctct
    50941 ggatgttctg actgccatgg ggctcctaga actctatcgt gcagatgaat taacaggttt
    51001 ggcgctacaa gcccctctga ccgtaaaaag ttttctagcg aaacccttgt ataagaactt
    51061 gttggcaagg aagattgggg agtcaagtat ggagaatctg cttgttcacc aacccaatca
    51121 agaacaaaat atttcaaaaa cattttctca ggtctttacc atggatgggc aggctccagt
    51181 tgtttttgaa ccgaaacatg attttgactg gtctgccttc aaggcattaa tggctaaaga
    51241 taagcttctt tttcaggatg aagctgagga tattattgcg cttagttaca tagcagagca
    51301 ggcaggttgg acctggttgg aaacctatcg gcaggctaaa gcaacagcta ttggacagat
    51361 gatctcgacc aaacgcttgc agcagtcacg tcagtcattg ccaacagaaa gcgccaacct
    51421 cactgcccaa gaacaagcca ttgttcgtga agcgaaatca aaatccagcc taaccttcct
    51481 gtcctttatc aaggagcaac ggaaggcagc ggtgacagga acagaacgaa aatgcttgac
    51541 ggatttggcc aatttgggtc tgcttgacga agttatcaat gtcttggtcc tctatacctt
    51601 taacaaggtg gactcagcca atctaaatga aaaatacgct atgaagctgg gcaatgattt
    51661 ttcctacaag ggaattggca gtgcagaggc agctgttctt tatctaagag agttaaagac
    51721 aggtcaagct gttaccaagt cgagccagcc acagaaaagc aatgtgcctg agtggagtaa
    51781 ggaagaggtt gtcatggata acagtcctga agctcaggca agaatggaag cgcttaggaa
    51841 acgaatgtta gaaaacgaaa gtaagggagg tagctgatga aatcagttca agacaggttg
    51901 tcgcaggcaa ccaatccaag ttcaaaatct tatcagcaac tctatcagga gatagtgagt
    51961 gatcctgaag tcgctgcttt tatcaaaaaa gaaggtttga ctcagcagga aatcaccctc
    52021 tccatttcta agtttttgga atatatcagt cagcgtgacc tttttgtcaa acaagatgag
    52081 acttatattg caaaaggcta tcaacctgtt ttggttatga atgagggcta tgcagatgtg
    52141 tcctatcttg aaacagagga attggtcgaa taccgtcgtt tggaagctat taagaaccgc
    52201 attcagctca tcaatatgcc tcaggctctc aaaaaagtta ctatcgctga tttagatttc
    52261 acagacagta atcgtataga ggtctatgat gccattgatg cttttgttga acaggtcggc
    52321 gaacactata aaggtttgta tctatatggt gattttggaa ttgggaagag ttttttgatg
    52381 gcttacatgg cccatcgcct atcaaccaat cgccacctct cgaccaccat tctgcattat
    52441 ccgacctttg ttgtcgatat aaaaaatgcc atcaaagatg gctctgtcaa ggagcggatt
    52501 gatgaaatca agctggctca ggtcttggta ctagatgata tcggagcgga gcaacatagt
    52561 ccgtgggtgc gcgacgatgt tttacaggtc attcttcaat accgcatgca ggaaaatctg
    52621 ccaaccttct ttacttccaa tttctccttt gatgagttgg aacggcattt tgcatctggc
    52681 aagtctggtg atgagacctg gcaggcccaa cgggttatgg agcggattcg ctatctggct
    52741 cgcgatttgc atttgaaagg aaataaccgc cgatgaatga aaccattgat ttgatgctga
    52801 atcatacttc cgtccgtcgt tttacggaag agcccattga ggtggaacat ttgcaagcta
    52861 ttatatcagc tgggcgagct gcatctagct ggaagaattt ccagtcttat tccatcattg
    52921 tggtgcagtc ggagaagaaa aaaaggcact ctatgacctg gtgccccagc cagctatttt
    52981 acaggctcag gctattctgg tttttgtggg cgaccataat cgtgccagca aggcggctca
    53041 actccatggt tcagactttg atgccaaagg aacagagaat ctcctgattt cttctgttga
    53101 tgcgagccta gcagggcaaa acgccctctt ggcagctgaa agtctgggct acggcggggt
    53161 ctttatcggt atgattcgcc ataaggccat ggcaatagca gagctgttca acttgccaga
    53221 ttatacctat cccattttct gtattgcctt gggtcgacca gcccaaaacc atcctgttaa
    53281 accacgtttg gaaagcgaag ccattgtctt tcaggaagaa tatgtggagc aaggcgtgga
    53341 agtaatccaa gcctacgacc aagttcagac agcctatgca ggtgcccgtc agacagaatc
    53401 ctggtctgag cgtatggttg cccagtttgg tcaggctgaa caaccagaaa caaagtctat
    53461 cttagaaaag aataaattat tgtaaactag aagaggagga gctatggctc taccaactat
    53521 cgccatcgtt ggccgtccca acgttggtaa atcaacacta tttaaccgta ttgcggggga
    53581 acgcatttct attgtagaag acgttgaagg tgtgacccgc gaccgtattt atgctactgg
    53641 tgagtggctc aaccgtaagt tttccctgat tgatacaggt ggtattgatg atgtggatgc
    53701 acctttcatg gagcaaatca agcatcaggc tgagattgcc atggatgaag cggatgttat
    53761 cgtctttgtc gtatcaggca aggaaggtgt gacggatgct gacgagtatg tatcgcgtat
    53821 cctttataag acaaacaaac cagttatttt ggttgtcaat aaagtagata accctgaaat
    53881 gcgtaatgac atctacgatt tctattcact cggtcttggc gatccttatc cagtatcttc
    53941 tgtacacggt atcggaacag gggatgtctt ggacgctatc attgaaaacc taccagctca
    54001 ggaagcagaa gaaaatccag atattatcaa gttcagcttg attggtcgtc caaacgttgg
    54061 taaatcaagt ttgattaacg ctattttggg tgaagagcgc gtgattgcgt cgcctgttgc
    54121 tggaacgact cgtgatgcta ttgatacgca ctttacagac ccagaaggtc aagaatttac
    54181 catgattgat accgcaggga tgcgcaagtc tggtaaggtc tatgaaaata ccgagaaata
    54241 ctctgttatg cgtgccatgc gtgccattga acgctcggat gtcatattga tggttatcaa
    54301 tgccgaagaa ggtatccgtg agtatgacaa acgcatcgca ggctttgccc atgaagctgg
    54361 taagggaatg attatcgtag tcaacaagtg ggatacgctt gaaaaagaca accataccat
    54421 gaagcagtgg gaagacgata ttcgtgacca gttccaatac ttgtcttacg caccgattat
    54481 ctttgtgtca gccttgacca aacaacgctt gcataagttg cctgagatga ttaaggccat
    54541 cagtgagagc cagaataccc gtattccgtc ggctgttctc aacgatgtta tcatggacgc
    54601 cattgccatc aacccaacac cgaccgacaa gggcaaacgc ctcaagattt tctatgcgac
    54661 tcaggttgcg accaaaccac cgacctttgt ggtctttgtc aacgaagaag agctcatgca
    54721 cttctcctat atgcgtttct tggaaaatca aatccgcaag gcctttgtat ttgaagggac
    54781 accgattcat ctgattgcac ggaaacggaa ataaagaggg caacctcttt tttctttgca
    54841 cctattcgta aatatgctag aatggacttg gaggtgttgg tatggtagcg gttccgattt
    54901 ggcgttcttt tttgatattg ctttcttttt catcattttt tcttgtattt tatgaagaca
    54961 gtcagtataa acctttcggc tttcgttatt ggctggggct agtggcttgt atctggctca
    55021 atttggtgac tttggcatcc tattttatcg cctttactgg tggttcaatt atggtctata
    55081 atcagtataa acagccattg gttgtgatat tccttttttg tctattcgtg gctagtatgc
    55141 taaccttact ttcctttaag gctatcaaga tgatgattcg acgaaccaaa tattatcgac
    55201 aggtaagaaa ggtaggctag tatggaattt ttgggtgagt tgctagttga gtttttgact
    55261 ggtctagcag acttcgatga aaagaaacac cctccctttg gaattcgcta ttggctgggc
    55321 tggttgggag ttctgattca tgttttactc cttgccttgc ttacttgtgt gactgtcttc
    55381 tttttcaagt ttttcttggt tggaaaaggc ttgattaacg tagtagtggc agttgttttc
    55441 ctgctatttg ccctattttg gctttggaaa ttcggaaaaa caatcttgaa aatgtggcaa
    55501 gcaacgattt attatttagc tattcattag agacaccagt ttacctgctg gtgttttttg
    55561 atacctattt aaacaattca aattgaaaat atgctataat gaaaagataa tattttagat
    55621 atattttaca gaagagctga tgattggttg aaggataaag gagttagaat gggtcgcatg
    55681 atgccagggc ggattcgcca agaaggaatt gatttgtacg aggctggaaa actgactgtt
    55741 cttcatagtg agaatggaaa gatggtgctg gatattgctg gtgagcgctt tacttacggc
    55801 gatgcagata gcgatttaca gtgtagttgc cagttatttc aaagtaaagg ttactgtcaa
    55861 catctagctg ccacggagta ttttttaaaa aatgatgcct caggcaagga tatgaagcag
    55921 tctttgaaag aagatggtga acaacataag gaaaccgtcc gtcggactta ttttggcggt
    55981 ctctttttag atgaaatttt gcggccagaa cctgaactgg ggattaagta tcagttatca
    56041 gttgaaggga gcttattgcc ctatgatcgt cagatagatt ggaccttgaa aattacccgc
    56101 ttgccagata cacgttcgta cattgtgcgg gacatcggag cttttttgcg cattgtgaaa
    56161 gccaacggtc actatcagat aggtaaaaat tattatgagc aagttactta tgaaaatttt
    56221 gatgaaccaa gtcaggctct cctggatttt ctctgggcct tggtaccaga gaaagttggt
    56281 atagattcgg atattcttac tcattttgga cgacattttc gtctgccaca agcctatttt
    56341 gaggaggggt tggagctgtt aaatcagcta gaacatttta acttttccta tcaacagcag
    56401 tcctattcct ccttgatggt tcttccgttg acaggtgagg aagggctcta tcattttgag
    56461 gtgaccgttc acacgcagat gattgaaatg gttattcacg aaaaaacagt ccgaccactt
    56521 tttcatgggc gctatctctt agtaaatggc acggtttata gtgtcaatcg ccatcaagaa
    56581 catctgattt accaaatttc cgaacttgtt ccaaccgaat caggtttgag aaaagtgcag
    56641 gtagatttcc ctgaccaaaa tcgtctggct ttaagcttgc tggatttgca gacaattggg
    56701 actgttaagg cacctaaacg cttcatcatt catgatttca agccagaatt tcatattcag
    56761 atggaggcaa atgacagttt atcactccaa ttggttttgg attttgatgg acgtaaggta
    56821 aggagtgaag aggatttggc tctcctgcct tttgccagcc atttccaaca tttggagagg
    56881 gtctatcagg ctattcgttt agctggcttt agggggacat atcatgccca gagagcggct
    56941 cttagtcagc aggagctcta tccatttttc caacaacaat tgcctatatt acaaaaaatg
    57001 gggcaggttc atctagcaga aaggctacag gctttatata ttgaagccaa acctcaatta
    57061 gaaattgtgc gtaacggctc acttttggat atttcgtttg acttatcagg tattgaacaa
    57121 tctgaaatcg atcaggcgat tacagccttg ttgaatcaag aggatcacta tactagcccg
    57181 agcggtaagg tatttgtttt tgatgaggaa accaagaaaa tcagtcaaac cctaatctat
    57241 ttgcgggccc gtcatggtaa ggaggggcag gtacaagtcc atgctcttgc cggttaccaa
    57301 ttggctcaat ccttgtcaca attcaaccaa gtcagtttct ccaaggaatt tgaggaaatg
    57361 gcaagctatc tagctcagcc agatttgttc ccgcttcctg ctgtggaagt aacaacgtcg
    57421 cttagggatt atcagcagac agggcttaag tggttgacta tgctggatac gtatggtttt
    57481 ggcggaattt tagctgacga catggggctt ggaaagacct tgcaaactat cgcctttctg
    57541 tccagtcgga tgacaaaaga ttccaaagtt ttaatattgg caccgtctag cttgatttat
    57601 aactggttag atgagtgtaa gcggtttgct ccaagtttag atgtggctgt tgtccatgga
    57661 aacaaggaac agcgtgaaga gatcattgcc aatgatcacc aagtcttagt tacctcctat
    57721 ccatccttca gacaggatgt tgcactctat agacaggaac gctttgacta cctcatttta
    57781 gatgaggcgc aggtcatgaa gaatgcccaa tctaagattg cacagcttct gcgtgagttt
    57841 gaggttggga attgctttgc cctctcaggt acgccgattg aaaaccattt gacggaacta
    57901 tggtccattt tccaaattgt cttaccaggt ttattgccag gcaaacaaga cttcggtaaa
    57961 ttagcagcca aggacattgc gcggaccatt caacccttcg ttctcagacg tcataaggaa
    58021 gacgttcttc aggaattgcc tgatttgata gaggtcaatg tacttaatga actgacagat
    58081 gaacaaaaag ctatctacct agcccagctt caacaaatgc ggactcaaat agctggcgca
    58141 gatgatgctc aaatcaatcg tagcaagata gaaatcctat caggaattac tcgcttacgt
    58201 caaatctgcg atactcccag ccttttcatg gaagagttta gtggtgagag tggcaaactt
    58261 aatagtttga aagaattact cttgcaacta aaagaaggtg aacacagggt cttgatattt
    58321 tcccaattca gaaatatgct ggaaaaaata gaagaacagc tgatagaaat tgggatgact
    58381 tcctatactc tgactggttc aacccctgct aaccaacgcc aagaaatgac acaggccttc
    58441 aatgcaggaa gtcgagatgc ctttctcatt tccttgaaag caggtggcgt tggcttgaat
    58501 ttgacagggg cagacacagt catcctagtc gacctttggt ggaatcctgc cgtggaagct
    58561 caggctatca gtcgagctca tcgcatgggg cagactgaaa aggttgaatg ctatcgcctg
    58621 attactagag ggacaatcga agagaagatt caagaattac aagaaaataa aaagaacctt
    58681 gttaaaacag ttcttgatgg caatgaaagc cgagcgaatt tgacggttga ggatattcgt
    58741 gagattttag ggattgagtg atatggaatc agttgactca gcgtagaatt aggaaataag
    58801 gcctaagtat ggtataataa gggttcgaaa ggatgactta tgattagaca tgaaaaaaga
    58861 tttccactag tcgcagatga tgaggtacta gttggagaaa atccaattat gagtttatat
    58921 gatgagagcg acctgattag caatatcagg ggaccttatc aagaaaagga atttagctgg
    58981 tctaccgatt cacagcgagt tgcgtcggca aaaccagtag cacaaacgga agatgagctc
    59041 ctgcctcctc tgtttgaagc taagccaagc cattattctc gcaaggaacg ccttcagcaa
    59101 ctgactaaaa cgaagccttc tccagtgaaa acacaggggc agttggctcg tgaacaggct
    59161 agagaagact tgaagaaaaa acgatcagca acctacttaa gagatgaaaa accagcacca
    59221 gctaaagttg tggtgaaacc tcctgtcgtt tcaacagctg aatctaaggc aggtcggttg
    59281 acacgtttgg cggacaacct acgtcagaca gactatattc tcgctgatat gccagctgtc
    59341 tattctttga agaaagaaga tagagaacag gagcaggtta taaagaaaaa ttcgtatgac
    59401 tttttaagaa agagtcaggt ttacaattat cctgagcgca gacaacaacg agatcgccaa
    59461 gtggcacaag aattgaattt gacacatata gaagaggaat aacatgacaa aaacctatca
    59521 ttttatcgga attaagggat cgggtatgag cgcacttgct cttatgttgc atcaaatggg
    59581 gcataaggtt caaggtagtg atgtagagaa atactacttt acacaacgcg ggcttgaaca
    59641 agctgggatt cagattttac cgtttgatga gaaaaatatt acagcagatg ttgaattgat
    59701 tgctggtaat gcttttcgtc cagataacaa tgtggaaatc gcttatgctg atgcccaggg
    59761 ctatacctac aaacgttacc atgaattttt aggtgaattt atgaaaggtt ttacgagcct
    59821 cggtgttgct ggtgcccatg gtaagacctc tacaacggga ttattggcac atgttatgcg
    59881 caatatcaca gatacttcct tcttgattgg tgatggtact ggtcgcggtt cggcaaatgc
    59941 tcagtatttt gtgttcgaat ccgatgaata cgaacgtcac tttgccccat accatcctga
    60001 atacagcatc attaccaata ttgattttga ccatccagat tatttcacta gtctagagga
    60061 tgttttcaat gctttcaatg attatgcgaa gcaagtaaaa aatgctctct tcgtttttgg
    60121 tgaagatgag caacttcgtc gtatcacggc aaatgctcca atctactatt atggcttgga
    60181 agacaataat gattttgttg cctatgactt gaagccttct acaagtggtt cacaattcaa
    60241 agttcgccat ggtgaagaag agttgggaaa attccaaatt ccaacctttg gtaagcataa
    60301 tgtgatgaat gcaacagctg ttattgccaa tctctatatt gctggatttg atttgcaact
    60361 ggttgcagaa cacttgaaga catttggagg tgtcaaacgt cgctttacgg agaaaattgt
    60421 caatgatact gttatcattg atgactttgc tcaccatcca acggaaatca ttgcaaccat
    60481 tgatgcggca cgtcagaagt atcctagcaa ggagttggta gccatcttcc aaccacatac
    60541 attcactcgt accattgcgc ttctcgatga gtttgcagat gctttgaatg gagcagatgc
    60601 agtttacctt gctcaaattt atggatcagc gcgtgaaaca gacaatggtc aggttaaggt
    60661 agaagatttg gcggctaaaa tcaacaaaaa aggtggactt gttacagttg aaaacacttc
    60721 accactttta gaccatgaca atgccgtata tgtatttatg ggagctggtg atattcagtc
    60781 ctatgaatat tccttcgaaa gactcttgtc aaatcttaca aataacgtcc aataaaaaga
    60841 aagacggagg cctatggccc ctgtttttcc gctatataag ggagaaaatt atggaaattc
    60901 gttttgctac tccttcggat ttagaacagg ttgttttgat tgaaaatgcc aatttttcta
    60961 aagaggaacg gatagctgag tctgttcttg ctacttatct gaatactttg agtaaaacct
    61021 gtttaataat ggaacacgat gaggaaatag caggatattt attatcctgt ccctctgttt
    61081 cgcagactgt tacagacgat attttttact tgacagagag tgacatgccg acaggcggtc
    61141 acctagctat tgccagtcta tccgtagcgg atgcctataa gggacaggga gtgggaactc
    61201 ttttgttggc agccataaaa gaagtggctt tagcgggagg atttgaagga gtttccctga
    61261 cctgtaagag gtatttgatt agctactatg aaatgaacca gtttgaagat tttggcccct
    61321 ccttgtcgca atttggagga caaaggtgga gtgatatgta ctggaaagcc ttgtagaaag
    61381 ggaagttctt gcaattcata agcctttaag atataatgta tagtaactgt gtgaaaggag
    61441 taacagcgtg acaaaggata cgaatgaaaa aaacacgcag tcttcaggct tccgcgatca
    61501 aattttacga gatttggaag aattgaaggt gaaacgtttg gcggaacagt cagcggatgt
    61561 tcttgttgat aagcgtaatg aagttatcaa tgagctccag cctagtcttg ccgaagaaaa
    61621 agccccccat agagagactg aaattgtaaa agtagaaggt attgctttcc ctgattcata
    61681 cttgtcagaa gaacagcaag aatcagaagt gatgacggaa agtattgaag agccaacatc
    61741 agaaactttg gctactccag agacttttgt gattgaaaaa gaagttgttt caagtccagt
    61801 tccacaagat actgttgaac gaaacttgga gaaattaagg aatcttgtgg ctgctaatac
    61861 ggcaggattt gatgaagtac catcaagctc gtcagtttct gagccagtag agtctgtcct
    61921 ttcagagaag tcttctttgg aagatacatt tttggaattt ccaacggaag atgtatcagc
    61981 tgtaacgggt gatacagaaa tcatcgcact agaggaaact attattgctc caaagacaac
    62041 ggctattgtt ccagaagtag acaagcagat gcaggaaaca caacccagaa gaagaacaac
    62101 ccacagaaca agcaagcaac gtcgtaaaaa acaagataat gccgctaaac acattgtctc
    62161 tgttatcatg tcgattgttg tagtggcagt cctagtgaca ggtgttactg gctatatgtg
    62221 ggtaaaatct agtctggaac cagttaatgc aaaagctaca gaaactattc aggtagagat
    62281 tccagaagga tcttcaactt tagaaattgg taaaatttta gttgacaaca agttgattaa
    62341 gaatgctact atttttaatt actactctaa aattaagagt tataataatt tccaaagtgg
    62401 tttttataat ttaaaacaaa atatgtctgt ggatgacatc gccaaagcac ttcaagaaag
    62461 cggaactccg acagctcaaa aagaagctgc agggaaggtt ttgattgttg aagggtatac
    62521 attaacacaa attgctcagg ctattacaga caatactaat acaaaagata agaacgataa
    62581 aacaccattt actgcagaac agtttatggc tactgtgacc aaccaagatt ttattaatcg
    62641 aatggttgct acttatccaa aattgtttgc cagcttacca gctgctgata gtggagttat
    62701 ttatcagttg gaaggttacc tcttcccagc ggtttatgaa tacagcgatg aaacaactat
    62761 cgaagagctc gttgagcaaa tgattgccgc gatggataat cgtttacaac catattatga
    62821 aacgattact gctaagaatt tgactgtaaa tgaagttctt accttggcat ctttggttga
    62881 aaaagaaggt tctactgatg aagatcgtcg aaacattgca agtgtcttct ttaaccgctt
    62941 gaatgcagca atgcctctac aatctaatat tgcaatcttg tatgctcagg gtaaacttgg
    63001 tcaagaaaca actctagcag aagatgcagc gattgataca tcaattgagt caccatacaa
    63061 catttattgg accccaggat tgatgcctgg accagtggat agtccaagcc tttcggctat
    63121 cgaggcagtg attaatgcca acacaacaga ctatctttac tttgtagcag atgttacaac
    63181 tggaaatgtt tactttacaa ataatattga tgaacataac caaaacgttg ctaagtatgt
    63241 aaatgcacat cttaataatg agtaaattct taaaacaaaa ttatgtggtt ttccacataa
    63301 ttttgttttc atgaaaaata aaagaaaagg aaagacaaat ggcagaaaaa acatatccaa
    63361 tgaccttgga agaaaaggaa aaattagaaa aagaactaga ggaactaaag ctagttcgtc
    63421 gaccagaaat tgttgaacgt attaaaattg ctcgttctta cggagacctt tcagaaaact
    63481 ctgagtacga agcagctaaa gatgaacagg cttttgttga aggtcaaatc tcaacaatcg
    63541 aaacaaaaat tcgctatgct gaaatcgtaa acagtgatgc agttgctgcg gatgaagtag
    63601 caattggtaa gactgtgact gttcaagaag ttggtgagac cgaggaagaa gtctatcata
    63661 tcgttggtgc tgcaggtgca gatgtctttg caaacaagat ttcaaacgaa agcccaattg
    63721 gccatgcttt gattggtaaa aaaacaggag atgttgcaac aattgaaaca cctgctggta
    63781 gctatgatgt aaaaatcttg aaggttgaaa aaacgaaata aggtgtggag agagtcgaaa
    63841 gattctctct ttttgtatag acaatatgta aagacctcta attgagtgtg gtaattcctg
    63901 ttagaagttg attatttttt tattctagtg tacaggtaaa tccacgaatt ctcaactggg
    63961 gatctttaca tagtgtctat ataagaaaac aaccatctga aatagatggt tgcgattttt
    64021 tatctagaac gttgtttacc ggcgttacgg tttctcttct tgttagatgt cttttcttca
    64081 ataactgctg aagcagttgg agtaacgtcc ttcgttttac gaggagttgc tgacactgtg
    64141 atatttttga attcctcttc cacttgttta cgaatacgag gtttcaaaat gaagtttgta
    64201 atagcttgtt gaagaatacc gaagagacca ccgacaaccc agtagagagt cactccagca
    64261 ggagacccaa atgagaacat catgatcata attggattca tcagagccat ggttttcatt
    64321 tgttttttct gttcttcatc catgccgact tgcattaaga gcgattgggc atagtataaa
    64381 ataccagcaa cgactgtcaa aatcaggcta gttgaaccta aattaatacc aaggaaagta
    64441 gcttctgaaa ttcctggtgt gtggcgagcc gcaaagtaga ttgcagagaa gaatggcatt
    64501 tgaatcagca tcggcaaaca accgattccg ccgagcatgc tcacaccgta ttgtttttga
    64561 gttgcaaaat actcttgttg cgcagcaagt tgctcttctt gagagcttgc atttttcatg
    64621 cgctcctgga ttgggcctaa aatcggtttg agataattca tcttctcaga ttggatagca
    64681 gctttccaag attggtaaat tcccaatggg aggatgatga atcgaaccaa taaggtgacg
    64741 atgataatag caagaccaaa tcctagagct tgatcttctg caaaaaagcg aatcaagttg
    64801 gacattggtt gtcccaacca atcccaaatg ggacctgtag gatttccgct tttatcagtt
    64861 tgaacacaac cagataagaa aagaagagtt gataaggcca tgcctgacaa gatgagtaga
    64921 cgtttatttt tcaatttttt tcgttccttt gtttttttca gtatactctc tattttactt
    64981 tttttgacgg aaatatacaa gttttatcta tagctaacct gaaaatcctt atagtcctca
    65041 aaattggcta gggtgacatc tagatagatg actttggcca tacgagaagg ccctttccga
    65101 atttcatgga taaaatggct gagcttctca gcattatctg actgagctaa aatggtgaca
    65161 gtcccatcat cattattcca aactctgccg tagatgtcgc ctatttccac agctaaaaat
    65221 tgaacagacc aacgaaagcc gactccttgc acacgaccag atgcaatcat ttttaccttt
    65281 cgcataagtt tctccttttg acaagacatt tactttatta tatcatggtt gggtaagact
    65341 gttcaaaaat caagataatt gatttagccg ttggaaaatt gaagttgctg agcagtaaaa
    65401 gaaaaggggc attcatggag atcattcgct caaaggccaa tcatttggtc aagcaggtta
    65461 agaaattaca acagaaaaaa taccgtactt cttcttattt gattgaaggc tggcatttgc
    65521 tggaggaagc gatggaggct ggagccaata ttgagcatat ttttgtggtg gaagagtatt
    65581 ttgaaaaggt tgcaggttta gccaatgtga cagttgtcag tcctgaaatt atgcaagaat
    65641 tagctgattc taagacgcct cagggggtag tagctcaact tgctttgccg agccaacgtt
    65701 tacctgagac tttggatgga aaatttttgg ttcttgaaga tgtgcaggac cctggaaatg
    65761 ttgggacaat gattcgtaca gctgatgcag ctggttttga tggagtattt ctatcggata
    65821 agtcggctga tatttataat atgaaagtgc ttcgttccat gcaggggagt cacttccatt
    65881 tgccagttta tcggatgcca atttcctcca ttcttacagc tcttaaaagc aatcagatac
    65941 aaatattggc aacgaccctc tctagtcagt cggttgacta taaggaaatt acgccacatt
    66001 ctagttttgc cttggtcatg ggaaacgaag gtcagggaat ttctgattta gtggctgatg
    66061 aggcggatca acttgttcat atcaccatgc cagggcaggc ggaaagcctc aatgtagcaa
    66121 ttgcggcggg tattctattg tttagcttta tttaagccaa ttgtactata gtgtggtata
    66181 atattccaac gaggttaacg aatggttgca tataaacaag ataaagaata catgcattac
    66241 gtggggcact tgattgccac acccaaggtg caaaaattgg ggaaaattcc tcatcattat
    66301 cactccacgc gcttggaaca ttccatcaat gtctcttata caagttataa gattgcgaaa
    66361 aaatttggtt gggatgccaa gtcaacagct cgtggtggtc tattgcatga tttattcttc
    66421 tatgactgga gagataccaa attcaacaag agccatgctt gggttcatcc acgaattgct
    66481 aaacgcaatg ctcagaaact cattcaactc aataaactag aagaagacat cattgtgaag
    66541 catatgtttg gtgcgacaat cgctcctcct tgctacaagg aatcctggat tgtgacatgc
    66601 gtggataagt attgggctgt gagagagtgg agtctgccct tacaacacaa gtggaaaaat
    66661 cgtaaggtct ttcggtttca ataagatgat ttaaaggaga atagtatgaa taacgattca
    66721 tttattatta atcaggtgga taataccgcc ctcaaccgtt tcttcggtaa aatttatggt
    66781 gtggttgcca tgggaattgg tctgtcagct ctagtttctt tcttggctgt gaccgtcttt
    66841 caatctttat tgcttagtct gctgagtgca ggttccatca tcatgatgct gattatgatt
    66901 ggtcagattg ccttggttgt ttctgcttca gcaatggcag ctaaaaatag cccaatggct
    66961 ctaccaatgt ttcttgccta ctcagtaaca aatggtatca ccattagcat gattttgatg
    67021 ttctatacta gtgaaacagt tgtgcttgcc tttgtgtcag cggctcttat gtttgctatc
    67081 atggctgtaa ttgggatgac aacgaagaag gatctttctg gtatggctca ggcattgcgt
    67141 gcagctctat ggggaattat tattgcaagt gttattaata tcttccttcg tagctctggt
    67201 ctaagcttta tgatgtctat catttctgtt ttggtattct caggtttgat tgcttatgac
    67261 aaccaacgta tccgcaatgt tttcgaacaa actggtggta atgttggcca agggtgggtc
    67321 gtatccatgg cgcttcagct ctatcttgac ttcatcaacc tcttcctcaa cctactccgt
    67381 atctttggtg gtttgagtag agactaatta tgacatataa aaaagaggat ttacctcttt
    67441 tttatatgtc gtttgttatg ctagagctcc cattggatcc catggggcaa ggacttgtgg
    67501 ttgagcttga taagctgcta gttcttcttg tgtgaggaat tcttttcgga caacaatctg
    67561 ataagtgtac tcgtccatcc agctatctga tgcgacgaag tatcccttgt caccaacctt
    67621 gtcaccccaa gagttttcta ctttccattt gagaggttgc tcattgtcat ctaaatcaac
    67681 accagttaag accatggcgt gggtcatcaa gctttcggag tagtctaatc ttccagcctt
    67741 gtcttgatgg aaatggatac ccagacctga agagaagtcg taggtgttgg ttgccatgat
    67801 acctgtttgg cgattgctgc tttgaccgac atcggaacca aaccaaacgg actcaccagc
    67861 tttgagttgg gcaatagcta gttctttaaa acggttcatt tctacattga ggtagcggac
    67921 agcaggggca ccaacgacgt tccccaataa ttcgactgta taagatttat tgtagggttt
    67981 gtcagttgta ggagcattga taatggagac atagtcagat agtttcaaac caacgtattt
    68041 ctcgtagaat gcctgaggtg tcacatttgt atcgcggtga tagacattgt ctttatctct
    68101 atatgcaaaa tcaaagctac gtggagggag gccaaggttg acggctagga agttaaagat
    68161 ttcttgaagc aagttttctt tttgtgtttg aacctcttcc ggagaagctc cttttgcaag
    68221 gaggtctcgc aagatttgtg catcttgacg gagcaatttg ttaaggtact gattgagctc
    68281 acggctggca ctggatgaga tagattctgg atagacggat tttggtacca caccatattt
    68341 ttcaaataag gccacaacca tatcccattg accgccatct tgttgagggg tatccaataa
    68401 gaattttacc ttacggctac caatttcttg gtcagctgta gcaatgattt gttcgaggaa
    68461 ccagttggac ttttcatatt tatcccaaaa gaaggtatgg gcctgtgata gctcaaaatt
    68521 ttccagtttg aagtcagaga tgagtttatg gcggaaggtg tttaatgcag caaacatcca
    68581 gcaacgtcca gaagcctttt ggtttgaaac agcgtccttg gtcaagtcga ttgaaaagac
    68641 atagtcattg tcgatggcgc tttggcgtgt ttctaaggat ttcaaaagtc cgttgtggct
    68701 aacggcattt tcggttgcct gtaggcttgg attagctaga tagttggcat ataagcgttc
    68761 tgtaaaatca aaatctaatg ttgacatata ttactccttt tttaatgata atcacattat
    68821 agcgctcata atttattttg caagtatttg gtggagttga aagctacagt tcctcacata
    68881 tttgtgatat aataggaaaa atatttgatg aggtagtgac atgaccaaga caccatttgt
    68941 tagtaaagaa gttttagaaa acattacgga gcaatttccg actccttttc atttgtatga
    69001 tgaaaaaggc attcgtgaga aggcccgtgc ccttaatgca gctttttcat ggaacaaagg
    69061 ctttaaagaa tattttgcgg tcaaagcgac accgacacca gcaattttaa aaatcttgca
    69121 agaagaaggt tgtggagtgg actgtgcaac ggatgtagaa gtgctgatga gtgaaaaatt
    69181 gggctttaaa gacatcatgt tcacatctaa tgacacccaa gcgcaagagt ttgtttatgc
    69241 cagaaaagtt ggggcgacca ttaaccttga tgcctatgag catattgagt ttttgaaaaa
    69301 tgttgctggt atcccagaaa cggtctgcct ccgttacaat ccaggtgggg tcttctcgct
    69361 gggaacggat attatggacc acccagaaga gtctaagttt ggcatgacca aggaccagct
    69421 catgaagggc tacaaggaat taaaagaact aggtgtgaag cagtttggta ttcatgcctt
    69481 tctagcttcg aatacagtaa caaatgacta ctaccctgtc ttagctcgtc aactctttga
    69541 attggccttg gaaattcgtg aggaaacagg tgtgaccttg gacttcatca acctatcagg
    69601 tgggattgga gtcaactacc gtcctgagca agaaccaaac gacattgctg tcattggtga
    69661 gggagttcgt aaggtttacg aggaaattct cacaccagct ggtatgggac atgttaaaat
    69721 ctttacagaa ttgggtcgct ttatgttggc gccacacggt cacttgatta caaaggtgct
    69781 ccatcgtaag gaaacctatc ggacttatat tggtgtcgat gcatcggcag ccaacctcat
    69841 gcgtcctgcc ttctatggcg cctaccacca tatcaccaat atcacgcgcc cagatgcgcc
    69901 aatcgaagtg gtggatgtgg ctggttccct ttgtgaaaac aacgacaagt ttgcggtcaa
    69961 tcgtgaatta ccacgggtag aagtaggaga caccttggtc attcatgaca gtggggccca
    70021 cggcttctcc atgggctaca actacaacgg tcgtctgcgt tcttctgaaa tccttttgca
    70081 ggaagatggc acagcgcgga tgattcgtcg tgctgaaaca ccagaagact atttcgcaac
    70141 tatttacggt tttgattttg acaggtaagt cttggaaaag actagggaat ttggtataat
    70201 agggttattg aaagattgtt aaaaacaatc agaagtatac tttttagaag agtcaggaga
    70261 ttgacagatg aacttaggtt tagctatttt acttattgta ttggcatttg caggtggtgt
    70321 ggccttgggt atttacttgt cacgcagaca ggtagaaaac tacattgctg ataaaccaat
    70381 tttggatgag aatgctttgc gtttgatgat gagccaaatg gggcaaaaac caagcgaggc
    70441 aaaagtacaa caagtacttc gccaaatcaa gagccaacaa aaagtggcaa gcaagaaaaa
    70501 ataatcaaac tgggactggg atattcattc ccagatccca ttttttgagt agaaaagaaa
    70561 ggctagtcag attttctggc tattctcagt attatggata atcgaccaat aggtttttta
    70621 gattcaggtg ttgggggatt aacggttgcg cgtgagttaa tgcgccaact accccatgaa
    70681 gaaatcgttt atatcggaga ttcggcacgg gcaccttatg gaccccgtcc agcagaacaa
    70741 atcagagaat atacttggca gttggtcaat ttccttttga caaaaaatgt aaaaatgatt
    70801 gtctttgcct gcaatacggc aacagcggta gtctgggaag aagtcaagga aaagttggat
    70861 attcctgttt tgggtgttat tttaccaggt gcttcggcag ctattaaggc aacgcaaact
    70921 ggtaaggttg gtgtcttagg aacggctatg accattcaat ccgatatcta tcgtgaaaaa
    70981 attcaggccc tttctccaga aacgcaggta gacagcctag cctgtcccaa atttgcacca
    71041 ctggttgagt ccaatagcca ccaatccagt ttggccaaaa aggttgttta tgagacactt
    71101 cgaccacttg ttgggcaagt agataccttg gtcttgggat gtacccatta tcctctgctt
    71161 cgccctatca tccagaatgc catgggcaag gatgtcaagt tgattgacag tggggcagaa
    71221 tgtgcgcgag atatttctgt tttattaaat tattttcaaa tcaaccgcag ccgtaccgaa
    71281 aaggatattc agcacagatt ttacacaaca gctagtccgg cagcttttaa agaaatagct
    71341 gaaagctgga tgggcattga tattcatgtg gagcatgtag aactatgaca gacaagatat
    71401 acgaatacag agatgaacac aattggttta ttggaaaagc cagctttgcc aatctatttg
    71461 gttcatttgg tgaaaatggc agagagcagg aaatttacca gattggtcaa ttgtttgaca
    71521 aactaattgc tggtaattat gaagatgaga actttaacca gtgtgtcaac attgaagtga
    71581 tcaaacttca gtctgaattt gccctctttc aatttgcctg tgatactttg aatgagttaa
    71641 acaatcgtca gttcaaggcc ctccaacacc aaggagctat tctggtaact gagaatgaca
    71701 aacttctctt ggttcatctg ccacaagcag gggttagcac ggcagatttc ttcggtcaag
    71761 acaagggatt ggcaagcgtg ggagatacca tcttgattgc cacaaaaaat gaaggtaaga
    71821 ctaaggaatt ccgtaagttt tttgagcgtt ttggctacca ggtcgagaac ctcaacaact
    71881 atcctgacct gccagatgta gcagaaacag gtatgacttt tgaggaaaat gcacggctca
    71941 aggctgaaac catagcagag ctgacaggca agatggtctt ggcagatgat tctggtttga
    72001 atgttgatgc tttaggtggc ctgccaggag tttggtctgc ccgtttttca ggtccagatg
    72061 ccacagatga gctcaataat gccaagctct tgcacgaatt ggccatggta tttgaactga
    72121 aagatcgttc ggcacaattc cattgtactc tggtcatggc tgctccaaat cgtgatagtt
    72181 tggttgtcga agcggactgg gaaggcttta tcggtatgga cctccgtggg gaaaatggat
    72241 ttggatatga tccacttttc ctcgtaggcg aaacaggtaa gacctcggca gaattaaccc
    72301 tagaagaaaa aaataagatt tcccaccgtg cacaagcact agaaaaatta gtggaggcat
    72361 ttccagtatg gcaggagcaa gcaaaacaat cctagtcatg agcgactctc atggggatag
    72421 acagattgta gaggatatta aacatcatta tcttagcaag gtcgatgcca tctttcacaa
    72481 tggtgattcg gagctggata gtcaagatag tttgtgggac ggcattcaag tggtcaatgg
    72541 gaactgcgac tattttggtg gttaccctga ccagctcatt actcaattgg atggtgtgac
    72601 tatcgctcag actcatggtc atctttatgg cattaattac ggctggcagc ggttggatta
    72661 ttgggcccag gaagttgatg cggatatttg tttgtatggg catctgcatg tgcctgatgc
    72721 cgaagtgcgt ggcaagaccc tcttcctcaa tcctggctcc gttagtcaac ctcgtggttt
    72781 ggtcagagaa tgtctctatg ctcttgtgac catctatgat gatcacttcc atgtggatta
    72841 ctacaatcga cagcaccaac tttacccagc attgacaaag gatatttcaa gatgattgca
    72901 agagaatttg aagccttttt attggaccag gaagaaactt tcttgacacc agcggataaa
    72961 ttggcagtaa ttattgatac gcataatatt gaccatgcaa aactgttact cagtcacatg
    73021 acctattcac gcgtacctgt ggtaacagaa gatggccgct tttttggaac catcggcttg
    73081 acggaaatca ttaaatatca agctgaaaat gaactatcgg atgatgagtt aaacagagat
    73141 atttctgtga ttgtaaagac agatgaagag acggttggtc tggattatga tttgacagag
    73201 gtgatgcgta agctagtaga tcaatctttc ttacccgtcc ttggggaaaa tagagaattt
    73261 atgggaatca ttacacgaaa atccatctta aaggcaatca atgctctctt gcataatttc
    73321 cccctagcat caaaagaagg caagaaatga agcaggcgat tgaatctttt atccagagca
    73381 aaaaggtcag tgtcaacagc caaaagtcct atacctatga cctgcagcag tttgtgacag
    73441 taacaaaggg ggagattagc caacagtctc ttttggtcta tcagcagtct cttctagact
    73501 tgaaacctgc tgctcagaaa agaaaaatgt cagcggtcaa tcaatttctc tactttcttt
    73561 atgaaaataa tctcctggat cgtttttata aattgcaaac gacgtcaggt ccagccagcg
    73621 tcaaaaagaa actggagcgt gaggatttaa ctctcctctt tcaagaaagt ccttggctag
    73681 aaggtcagct catcgcactt ttgatagcat atttaggttt gacgccaagt gaaatagcag
    73741 agctgactag tcagcaggtc aatctggatt ttcaagtcct gacagttgag aagggaggag
    73801 ccaagcgggt gctcactctg cccaaggaat tgatacccta tatggagtct catctgtctg
    73861 gacgctatgt cttcgataag aagggacaga cctattctcg tcaatggttt tttaatcgtc
    73921 tgacagaatt tgtccagtcc atcggcaagc ctgattggac agcccaaaaa ttgcgagagc
    73981 agtatattct aaaacagata gacgaaggaa aatccttgga ccaaatcgcc aagcagttgg
    74041 gactgaaaac aagcatgagt ttggaaaaat ttagataatg gatataaaat taaaagattt
    74101 tgaagggcca cttgatctcc tgctacacct ggtgtccaag tatcaagtag atatttacga
    74161 ggttccgatt acagaggtta ttgagcaata tttggcctac attgctacct tgcaagccat
    74221 gcgtttagag gtagcgggag aatatatgct catggctagt caattgatgg tcatcaaaag
    74281 tcgtcggtta ttgccgaagg ttgttgagca aattgatccc gaagatgatc ctgaaatgga
    74341 cctcttggac cagttggaag aataccgcaa gttcaaactt ctcagtgaga aattagggga
    74401 gcaacatgat gaacgggcca actatttttc aaaacccaaa ttagatttga tttatgatga
    74461 tgtacagtta gccaaggata agaccgtcat tgacattttc ctagcttttt ctaaagtaat
    74521 ggctgaaaaa caggcaagcc tccgtcagtc tcatgcgacc attgcccgtg atgaatataa
    74581 aatcgaagac atgatggact ttgttcgcag tcgtttcgag actggtccac gccttgaact
    74641 ccgtcagctt tttcaagaaa gtcaagatgt caatgaaatg attacgatct tccttgcgac
    74701 tttggaattg gtcaaggttc atgaaatcgt cctggagcag acagagactt ttggagatat
    74761 ttatctagta aggagtgaag atgaatcgct tagctgaaat cgaagtcctg ctctttgtgg
    74821 caggtgagga tggcttgacg ttacgaaatt tggcagagat gttggaaatg cagccgacag
    74881 ctgtcagcca gcagttagag aaacttagtg agaaatatat ggcagacaaa gcctcaggct
    74941 tagccatctt ggagtcttcc aatcgctaca agttggttac taaaaaagaa tatgcaaacc
    75001 ttttgcgaat ctatgccaaa acaccaatca atcagactat gtcacgggct cttttggaaa
    75061 ccttatcaat cgtagcctat aagcaaccca ttactcggat tgaagttgat gatattcgcg
    75121 gagtcaattc cagtggtgcc atcagtaaat tacaggcctt tgacctcatt cgtgagaacg
    75181 gtaagaaaga ggttcttggt agaccaaatc tctatgtgac aacggattat ttcctggatt
    75241 acatgggaat taatagtcta gaagaattgc ctgatgtgtc tgaaatagat ttgattcaag
    75301 aggaaacaga gctattcgta gaaagaaatg agttagaaaa tgagaattaa taaatacatt
    75361 gcccatgctg gagttgccag tcgtcgtaag gcagaagaac taatcaaaca aggactagtt
    75421 actgtaaatg gtcaggttgt tcgtgagcta gggacagtta tcaaatcggg tgataaggta
    75481 gaggttgagg gacaaccaat ttataacgaa gagaaggtct actatctcct caataaacca
    75541 cgaggtgtta tttctagtgt gtcagacgac aagggaagaa agacagttgt ggacttgttg
    75601 ccaacagtta aggaacggat ttatccagtt ggacgtctgg actgggatac atctggggtc
    75661 ttgattttga ccaatgacgg tgattttaca gacgaaatga ttcacccacg taatgagatt
    75721 gataaggttt acttggcgcg tgtcaaaggg gttgccaaca aggaagtttt gcgtcccttg
    75781 acccgtggtg ttgtcattga tggcaaaaag actaagcctg cggtgtatga gattataaag
    75841 gtggaccctg ttaagaaccg ctcagttgta gaattaacaa tccatgaagg tcgaaaccac
    75901 caggttaaga agatgtttga ggctgtcggc ttgcaggtag acaagctgtc tcgtactcgc
    75961 tttggtaatc ttgacttgac cggcctacgt ccaggagaat accgcaagct caataagaaa
    76021 gacatcagta aactacatac cctggccgtt actaagccag ttaaaaaata atgactagac
    76081 ttttgatcgg cttggtccga ttctatcaga aatttatttc tccccttttt ccgccctctt
    76141 gtcgctaccg tccaacttgt tcggcttata tgatcgaggc cttacaaaaa catggactca
    76201 aaggcttttt gatggggttg gctcgaattg gtcgctgtca tcccttggtg gaaggtggag
    76261 aagatcctgt gccggacagg tttagtttga aaagaaataa gcagaagtag ggcaacctac
    76321 tttttgttct gtataaatat aggtttttag gttgccttta gtactctctc aaaagatttg
    76381 ggagcctttt aaggctggag atagggcgaa gcaagttcgc ctcaatatta acagtccact
    76441 ggactgtaaa agccaatcaa tcactgcgct cagatgttga cacgaactct gagaagcaag
    76501 gttggtcttt ctgcccagcc tccatgattt tctatgaaaa tttgaaaaaa atggggcaaa
    76561 actcttgatt ttcagccttc tttaagctac aatagtatca cgatttcatt gatttaactc
    76621 aaacctgttg tgagttaagt gaatgaatca tcattcacca tgctgtttgc tgagcttgac
    76681 tccgggcagt gtggctattt ttttgtcttt cttgccatag gagacagtcg agtcttttta
    76741 ctcaatgaaa atcaaaaaaa gaaactagac aacaagcaac agaccgtact ggtatacggc
    76801 aaaatgaagc tgacaatggt ttggatttga ttttcgaagg gtattcgtgt ttagtgaaat
    76861 gtcaccagag aggttcttat gaatattgaa acattagagt atatagaaga aacagccaaa
    76921 aaccacattg tcttatttga accacagatt ccacagaata caggcaatat tgctcgaact
    76981 tgtgctgcaa ccaatagtcc tctccatatc attaagccta tgggatttcc aattgatgac
    77041 cgtaagatga aacgtgcagg actagattat tgggataagt tagatgttcg cttctatgac
    77101 aatttagcag aattcatgga ttatgccagc aaacaaggac gtgttcactt agtatctaaa
    77161 tttgctgata aaacctattc ggatgaagac tataatgatg ggaacattca ttatttctta
    77221 tttggtcgtg aagataaggg cttgccagaa gattttatgc gtgaacatcc agagaaggct
    77281 atccgtattc caatgaatga tgaacatgtg cgtagtctta atgtctctaa tacagcgtgt
    77341 atgattgtat atgaggctct tcggcagcaa cagttcgctg gtctagattt ggttcacacc
    77401 tatgatgggg acaagttaaa atagtgaaaa cttaccaagc tcttgcaaat gcaaggcttt
    77461 tttggtatga tgaaatagtc ttcagggcag ggtgaaattc ccgaccggca gtgtaaaacg
    77521 tggtaatttg ttttacgact aggcagcgaa tcgaagacca tactgaatgt atgtcgagat
    77581 gagctaacgc agttttagaa caaaatagac cgttggtagt ctgcgagcga aagctgatgt
    77641 ggtgcaattc cacaaccgac agtaaagtct ggatgggaga agacgaagtg gaggcctttt
    77701 gcctgctctt ccacgcttct tcaattttca aaattggagg aaccttatga caaacacacg
    77761 caaaatgacg attatcgcca ttctctcagc agtatccttt ctgctcatgt atttgaaatt
    77821 tccactgatt ccaacagcca gtttcctgga agtggatttt tctctcgtac cgattttatt
    77881 tggtctgctt attctagata tgaagtctag ttttgctatc cttttggtac gaacttttct
    77941 caaactgatt ttaaataatc agggaccatc gacaattatt ggcttaccga tgaatattgc
    78001 agctatgtca gttttcattt tagcagtagc ttatttctgg aaaaatgaac aaacgttaaa
    78061 aaattattta aaagcagccg ctgttgcgac aattggttcg acagcagtga tgctggcatt
    78121 aaattacgtt tatgcggtgc ctgtttatgc tgcttttgca aattttgata ttaaagaaat
    78181 cttgggtctg actaactatc tttttatgat ggtacttcct tttaatcttt tacaagggat
    78241 tgtgctttcg gcaatatttt atatctgtta caaagcagcc caacctattt taaagaaagt
    78301 ttaaccttta taaggtaaac tatactgtat gaagaataaa caaatccatt tacgaaacgc
    78361 atcttttttt gctctggcct ttgtgacatt g
//
LOCUS       DBNFCK010000002        68914 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.002, whole genome shotgun sequence.
ACCESSION   DBNFCK010000002 DBNFCK010000000
VERSION     DBNFCK010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 68914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68914
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.002"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..938
                     /locus_tag="AC1PIW_000082"
     CDS             <1..938
                     /locus_tag="AC1PIW_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIP6605060.1"
                     /translation="KIIIELTNKCHLQCTHCFKEAGPINRNFLKYEELINFLDRVKGK
                     VYEIQLTGGEPMAHPHFKEISKYATENFQEVTMTTTGHLINSKSIIYLKGMSYIQISL
                     YHHNPILNDQITNGKDTLNKTIRGIRFLNNENLDYSVTNIVRNSLIDEFDNFIEFLIL
                     NQVKAVRFGLFSHLGRGKLVGSDWFLSPSKVEEFYEMLIEKSREYGSRINIHTWEEDN
                     CFNYFEDIVDGTMRCGAGVIEWTINESGHIKPCTFFPDGEFSSYSLQNFEEYSMQNHE
                     QNIVRKINDWEGLLQTVGLSTRNICEEIYKMVEKK"
     gene            935..2512
                     /locus_tag="AC1PIW_000083"
     CDS             935..2512
                     /locus_tag="AC1PIW_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIP6605061.1"
                     /translation="MKKRNFWLTLSCIFAVALLNVGFLYQYRYIIDAIAAGDRQAFVS
                     YFVQMGLTVLVMLVFEYIRQIANVSYLNQVGFQLQATFIGKIFDLPFRQFVEQGAGAY
                     ISQLNNDLETVKEDYYDAFFTIFQGACTFGIASLALLSLDGLTAIFLILISFLPVVIP
                     YLFKKRRRLNQEAISDSQQVYNTRVSDVFLSYLQVKNSHQRQQVLDGLNDRYQAVNDK
                     VNQATRTTVTMRLLVGFVFYLTTLSIIFIGGFQVFSGALTIGGLTAILTISEQLVDPI
                     NSIAAAFLDRHAVKKLKQEFELSTFGQETKGQELASAFQSIQLVKASYAVGEKQVFED
                     LTVAFERGKKYLILGESGSGKSSLALILTKNSQLQAGDIYVDQISLSDLSYQAIQDKI
                     AYLPQEGSLFHDTVLYNLTMGREVPEDRLMFHIKTVNLDSRFPSYASLSEEISDDSGL
                     SGGQKQRLLLIRALLQDKDILLLDESLSALDQETYAVIENYLASLADKTLIHISHRVS
                     DEVLSRYDGVVRIGESN"
     gene            complement(2932..3013)
                     /locus_tag="AC1PIW_000084"
     tRNA            complement(2932..3013)
                     /locus_tag="AC1PIW_000084"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2978..2980),aa:Leu,seq:cag)
     gene            3409..4284
                     /gene="rsgA"
                     /locus_tag="AC1PIW_000085"
     CDS             3409..4284
                     /gene="rsgA"
                     /locus_tag="AC1PIW_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HIP6605062.1"
                     /translation="MQGRIIKALAGFYYVEADGQIYQTRARGNFRKKGQTPYVGDFVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSAKEPDFNANLLDRFLVLLEQK
                     DMDPIIYISKMDLVEDRTEMDAFKAIYEKIGYPFVYSLEELMPLLQDKVTVFMGQTGV
                     GKSTLLNRIAPELALETGAISDSLGRGRHTTRAVSFYNVFGGKIADTPGFSSLDYEVK
                     EAEALTDCFPEIAESSQDCKFRTCTHTHEPDCAVKEAVASSAISQSRFDNYLQFLSEI
                     QNQRETYTKVSKKFK"
     gene            4299..4958
                     /gene="rpe"
                     /locus_tag="AC1PIW_000086"
     CDS             4299..4958
                     /gene="rpe"
                     /locus_tag="AC1PIW_000086"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIP6605063.1"
                     /translation="MSHFKIAPSILAADYANFEKELKRLEKTGVEYVHIDVMDGHFVP
                     NISFGADVVAAMRPHSKLVFDCHLMVSNPENHIETFARAGADILTIHAEATVHLHGTL
                     QKIRAAGMKVGVVINPGTPLVTIEPVLNLVDQVLLMTVNPGFGGQAYIPEVADKIADL
                     VKLREAKGLAFDIEVDGGIDDKTIHSAKSAGANVFVAGSYLFKGDLDANVAKLRAALN
                     D"
     gene            4951..5583
                     /locus_tag="AC1PIW_000087"
     CDS             4951..5583
                     /locus_tag="AC1PIW_000087"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HIP6605064.1"
                     /translation="MIKIAVIAGGSFDCLPEPADLYVGVDAGSLRLLDHSLPLDWAIG
                     DFDSVTSEELGQIREMAEQFLQAPAEKDDTDLELALKEIFKAYPQAQVRIYGALGGRM
                     DHMMSNLFLVAEPDLAAYMEQIELVDSQNVVRFRPAGSHRISPIAGMKYISFMPSDQS
                     HLTIRYAKYPLDASNYFFKKCYASNEFIDRDIDIQLDQGYVVLIYSKDKD"
     gene            5584..6822
                     /locus_tag="AC1PIW_000088"
     CDS             5584..6822
                     /locus_tag="AC1PIW_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HIP6605065.1"
                     /translation="MDIVLLILLILVLLALVFLYGKWQSLALLLQDQAEDTADNLSDQ
                     LSYQLENATLKQQQTIHQEVERLRTELYQQLTDIRQELNKSHLETRDATDLRLQAIQE
                     SNEKRLEEMRQTVEEKLEKTLQTRLQASFEMVSKQLESVNRGLGEMQTVARDVGSLNK
                     VLSGTKTRGIMGELQLGQIIEDILTPSQYEREFATVSGSSERVEYAVKLPGRTEGDYI
                     YLPIDSKFPLADYYRLEDAYESGDKDQIDLHRKNLLAAIKRFAKDIQSKYLNPPETTN
                     FGVLFLPTEGLYSEVVRNPIFFDELRRQENIVVAGPTTLSALLNSLSVGFKTLNIQRS
                     ADDISKVLGNVKLEFGKFSDLLVKAQKQLNQASSNIDKLLTTRTNAIERSLRTIDLYE
                     DDQTKGLLGLSPLDEEDNEN"
     gene            6812..7753
                     /locus_tag="AC1PIW_000089"
     CDS             6812..7753
                     /locus_tag="AC1PIW_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HIP6605066.1"
                     /translation="MKINQMKKDEFFEGFYLIKSAEVRQTRAGKDYLALTFQDDTGEI
                     EGKIWDAQPGKIKDFTAGTVVHMQGCREVYNNTPQVNQLVLRLPKAGEPNDPADFKEK
                     PPVDVKDTKDYLSQMIFRIENATWQRIVRALYSKYDKEFYSYPAAKTNHHAFYSGLSF
                     HTATMVRLADKIGDIYPQLNKSLLFAGIMLHDLAKVIELTGPDNTGYTVRGNLIGHIS
                     LIDEEITKVLMELGIDDSLEEVTVLRHVILSHHGLLEYGSPVRPQIMEAEILHMIDNI
                     DAEMMMMLSALDKVGPGEMTNRIFAMDNRAFYKPNID"
     gene            7753..8619
                     /locus_tag="AC1PIW_000090"
     CDS             7753..8619
                     /locus_tag="AC1PIW_000090"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HIP6605067.1"
                     /translation="MAVRIVSRQPLTKGWSTDQKYKVQLEDGRLGLLRIAERPAYEAK
                     QLEFQLVENLFGLGLPVAEPLSFWADDLSVYTLYEWMEGQDMNDLATSLSEQTLYELG
                     CQSGKFLRTLHALPIDQSLRDWNSFYQAKIDNKLAAYQAASHSYPNGQAMIAFVQANR
                     HLLEGRPIAYHHGDFHTGNFLRGQDGRLKILDFDRYDIGDPWEEFNRLIFTADLSPAF
                     ARGQVDVYFDGAIPEEFWKLMALYVTVNSLGALSWAEQVDSEQIPLMKLQAQKISEWY
                     EYFNHHLPKWYM"
     gene            8721..9533
                     /gene="purR"
                     /locus_tag="AC1PIW_000091"
     CDS             8721..9533
                     /gene="purR"
                     /locus_tag="AC1PIW_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HIP6605068.1"
                     /translation="MKLRRSERMVVISNYLINHPYELTSLNTFAEKYESAKSSISEDI
                     AIIKKAFEESSIGHIETITGASGGVVFTPSISKAESVEIAQALRDQMAESNRILPGGY
                     IYSSDLLSTPHILKNVGRIIADAFKDEKIDAVMTVATKGVPLANAVANVLNVPFVIVR
                     RDLKITEGSTVSVNYVSGSSDRIEKMFLSKRSLKAGSRVLIVDDFLKNGGTINGMISL
                     LSEFDSTLVGVAVFAENQKGDRSVANYKSLLAVTDINVKENRVDVELGNIFE"
     gene            complement(9560..10426)
                     /locus_tag="AC1PIW_000092"
     CDS             complement(9560..10426)
                     /locus_tag="AC1PIW_000092"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIP6605069.1"
                     /translation="MKIDIRIPQAFPQLTVKEVLEDYFLIPRKIRHFLRTKKQVRVNG
                     ELINWQCPITAGDLLELTFDQEDYPEKSIPLGQADLVEELYQDEHLIIVNKPEGMKTH
                     GNDPTEIALLNHVSAYVGQTCYVVHRLDMETSGAILFAKNPFILPILNRLLEDKVIYR
                     DYLALCQGQLKMTDWTITDKIGRDRHDRRKRVVDNRKGQTALTQVRLLKTLGKNSLVS
                     CRLQTGRTHQIRVHLAHHGHALIGDPLYSRVAPPRLMLHAQKLSLTHPLTLEEISVEA
                     RSESFERIVKKS"
     gene            complement(10428..11396)
                     /locus_tag="AC1PIW_000093"
     CDS             complement(10428..11396)
                     /locus_tag="AC1PIW_000093"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIP6605070.1"
                     /translation="MTQVYDITIIGGGPVGLFAAFYAHLRQAKVKIIDSLPQLGGQPA
                     ILYPEKAILDIPAFPSLTGQELTDNLLAQLAPFDTTICLNETLTAIEPGETITLTTNK
                     GNHQTKTLIIAMGGGAFKPRPLEIDGADSFDNVHYHVSNIQQYADKDIVVLGGGDSAV
                     DWSLAFEKIAKTTQIIHRRDNFRALEHSVEELKQSSVTIHTPFIPKGLSGENGRASAI
                     DFDKVKSEDKLTLSFDHLFVNYGFKSSVGTLKEWGLELNRHRIVVNSKQETSVPGIYA
                     IGDCCFYEGKIDLIATGLGEAPTAVNNAMNYLNPNEKVQPKHSTSL"
     gene            11549..13762
                     /gene="pbp2a"
                     /locus_tag="AC1PIW_000094"
     CDS             11549..13762
                     /gene="pbp2a"
                     /locus_tag="AC1PIW_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP2A"
                     /protein_id="HIP6605071.1"
                     /translation="MDDLQHQPIEVPEDFQIDHEDRPSRRHRGNRGSIDKPKKKSRIP
                     EPIRKFWRRYQLTKILIILMVLTVLTVGGYLFFLAKTANVGDLQQALKATTIIYDKDG
                     AEAGTLSGQKGTYVELDAISDNLENAVIATEDRSFYKNSGINYQRTILAALTLGRSGG
                     GSTITQQLAKNAFLTQDQTISRKAREFFLALEINKKYSKQEILTMYLNNAYFGNGVWG
                     VEDASQKYFGIPASNLTLEQAAVIAGMLKGPEIYNPYYSLENAVNRRDTVLQNMVNAG
                     YIDQATADAGFQVDLASQLSDTYTGKQSNYSYPSYFDAVIAEAIERYGLKESDIINNG
                     YRIYTEMDQNSQASMQVIFNDETMFPTSSFDGTHAQAASVALDPRTGGVRALVGRVNS
                     SEDAVFRTFNFATQAKRSPGSTIKPLVAYAPAVAAGWSIDMALDNHTETYGDYTLHNY
                     DYSTSDTIPMYQALALSYNLPVAYIVNTLGIDKAFEYGQKFGLDMENVEKVLGVSIGS
                     GVETNPLQMAQAYATFANKGVMKDAHLITRIETASGKVLAEHRETSTKVIDRAVADKM
                     TAMMLGTYTNGTGVTADTANYHIAGKTGTTETSFDVNLVNDQWHIAYTPDLVISQWVG
                     FEQTDENHYIDSSSYWKAPSVFQTVANSILPYTAGSKFEVENAYAQNGIVEVAPDEAA
                     QTESSLGQEQVQEISEQAKNLIEQTKQNLIDAQLPERAKTLWDNITSWFGELSNP"
     gene            13796..13948
                     /gene="rpmG"
                     /locus_tag="AC1PIW_000095"
     CDS             13796..13948
                     /gene="rpmG"
                     /locus_tag="AC1PIW_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIP6605072.1"
                     /translation="MALKKASLACTVCGSRNYSISLSSNPKPTRLEVNKFCKHCGQYT
                     VHKETR"
     gene            13958..14134
                     /gene="secE"
                     /locus_tag="AC1PIW_000096"
     CDS             13958..14134
                     /gene="secE"
                     /locus_tag="AC1PIW_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HIP6605073.1"
                     /translation="MKFIKDIIRILKDTSWPTRKQSWTDFVSVVEYTAFFVAVVYLFD
                     LILSRGIMSLINLF"
     gene            14244..14783
                     /gene="nusG"
                     /locus_tag="AC1PIW_000097"
     CDS             14244..14783
                     /gene="nusG"
                     /locus_tag="AC1PIW_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HIP6605074.1"
                     /translation="MYDSFDKGWFVLQTYSGYENKVKENLLQRAHTYNMLENILRVEI
                     PTQTVQVEKNGEVKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKP
                     TPLLEEEIRQILVSMGQTVQEFDIDVKVGDTVRIIDGAFTDYTGKITEIDNNKVKMVI
                     SMFGNDTIAEVNLSQIAEL"
     gene            14927..20005
                     /locus_tag="AC1PIW_000098"
     CDS             14927..20005
                     /locus_tag="AC1PIW_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIP6605075.1"
                     /translation="MKQKWSQIENKQRFSIKKLSVGVASVSIGFFITGVPMVQADTSG
                     EGLESTVAVATDMDSRQNSAVEKKEDGPLSDDPVKTEQVDEPVAEEGVVEEVVDTEAG
                     EESGLLTDQAATEIETTAGKTTDESKEKEDISGKEASAPQTIPQESQLEPEEVTTGRY
                     ILQFSEENRNLVLDKLKKIDGVKIVHEYKEVLTGASVEVGKESLSDVKAITELTSLEE
                     SRRIRPTLHTAKQLVGALKASSKYQTDGRGMVIAVIDSGLDIKHKDMRLDDGVIPKIK
                     DITPSTTGTYTLKVPHGYNYVSGNDNLYDDTHEPHGMHIAGTLAGNATDEEVASKKGV
                     DGIAPNAQLLVYKIFSNDPKNYKAETEDAAYAAIEDAIKHGADVISLSVGYYDSGLPG
                     NAYYTIAKRAAEKGIIITAAIGNAGASSSDTSFDLHTNNALGAVDTATTVGVAATPAV
                     IAVGSARNTHLVQREFMLNGQSFGYYPIGYTTLTEGKYEFVDAGNGHWEEVQGLDLAG
                     KVAVIKKDKFDLKDAVRNLKFKDVAGIIVINTDQGWNKDYYRTHQLLVDDKTLLSYSS
                     IWGISLSGEDGRRLLEVANQSQGNTGLVLKPTIGMKKLIEVPTVSGFSSWGPTVNLEL
                     KPEIVAPGEDVYATLNDNRYGSMSGTSMASPIVAGASALLLPRIRQMTPPEGMTRMDL
                     LRIILMNTATPLVDVLDSSGHALENSPRQQGAGLLQIDRAFETDVILHHRLKGGVELK
                     EIGRETEFEVTLENLGNQQRSFAISAGKVLTSQDVPVDRIGRSGKVVKEIHATEIKGS
                     SIHLSEQSIQLGPKEKRTIRLKLDAGEAKDQFAEGYIYFKSLTEGQSDISIPYFGFVG
                     DWSKERIVDAPAWETSSKLKLTSVLSSYKHNKSGRYIELGREKIQDNQSPLNPDNIAI
                     QNQHSDSQIGNAFVRFALLRDITNYDLDIVKEATEDAPVLRRIDTGTMLSRVRYVDYF
                     ESLSEYSKLRTPIELHRWDGKVYDASNDENIPAPEGQYFFRLRVKNKENGAYQYTYLP
                     VKIDNQKPEIVAIDTNRLSSHRELVVTAKDNNKVWEVRANLNGEDLLVEKVVDDAGQL
                     HYHLKEVELPLDAKNHLRVEVMDIAGNVVAVEKDLMAPVIQFKNLEDLMAIRSKKTVE
                     IKANVSAQVSDVQANLDAQAVNYSLENGQLSLQIPEQSDGRHSFELILKDKDGNLIYT
                     KTLNYLVDNEKPTIDLDIEKDEEDEEVIQIGKNGRFTLKGKVSDNVSLPKDIKLYYSN
                     LDIGKGERKIIDVKEDGSFEQDFFKSDFPRAIMLTAVDEKGNKLKDLRINTSPESLDE
                     EEETEVPITVNNWLIDPIRFNKESLGRELDSGLVDFKKQEDGTYLFTFEIEAETEQAH
                     SVRINGGEKRYFEDGKLTYPVTLIEEGNVVDISVYNEADELTYTKKYQMLVDTENPVL
                     QLENEVLPLERQVVDSEEDEDEENQYAGVLLADADGHLTLTGSAKDNGIYWSLKINED
                     FVARGGFWRQYGNNEKAFRYELHSLKDGDTVKLDLSDSFGNAVVKKYKVRLNDKEVSE
                     QVPEKDLHVERSDKDQTPSIPILKSEAHIPMPKEENSLAPQTGSTEIALLTGDTREDG
                     VEHLGKLTKHEEPLGISDERIEVSVPHREFFERSGIGETGALAADTSGKLPQTGDSLG
                     SVFISTLLGLFGGAMALGNLKRKE"
     gene            complement(20048..20118)
                     /locus_tag="AC1PIW_000099"
     tRNA            complement(20048..20118)
                     /locus_tag="AC1PIW_000099"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20084..20086),aa:Cys,seq:gca)
     gene            20659..21171
                     /locus_tag="AC1PIW_000100"
     CDS             20659..21171
                     /locus_tag="AC1PIW_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIP6605076.1"
                     /translation="MIVLITGASHTGKTVLAQKLLEKYHYPYLSIDHLKMGLIRSGNT
                     KLSVEEDDKLTAYLWPIVREMIKTAIENEQNLIVEGIYIPFDWANDFATAYLDHIRYY
                     CLVMSENYIQKHFGSIKKHARLIENRIDDEWCTMESVLEENTRFFRRAKKYDANILWI
                     DESYEIEIDL"
     gene            21518..22294
                     /gene="rpsB"
                     /locus_tag="AC1PIW_000101"
     CDS             21518..22294
                     /gene="rpsB"
                     /locus_tag="AC1PIW_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HIP6605077.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKLADQAYEFIRDAAANDAVILFVGTKKQAAEAVKDEAIRAGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEINRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIADMPRIPDV
                     MFVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKMA
                     DAIIEGNQGEDSVAAVEAELAAEPASTESIEELVEVVEGK"
     gene            22450..23490
                     /gene="tsf"
                     /locus_tag="AC1PIW_000102"
     CDS             22450..23490
                     /gene="tsf"
                     /locus_tag="AC1PIW_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HIP6605078.1"
                     /translation="MAEITAALVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVDGNVAAVVEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPADNEAALKLAMPSGETLEEAYVNATATIGEKISFRRFALVEKTDAQAFGAYQHNG
                     GRIGVISVVDGGDETLAKQISMHIAAMKPTVLSYTELDEQFVKDELAQINHKIEQDNE
                     SRAMVNKPVLPLLKYGSKAQLTDEVIAAAEEAIKAELAAEGKPEKIWDKIIPGKMDRF
                     LLDNTQVDQAYTLLAQVYIMDDSKTVEAYLNSVNASVVEFARFEVGEGIEKASNDFEA
                     EVAATMAAALGK"
     gene            complement(23753..24454)
                     /locus_tag="AC1PIW_000103"
     CDS             complement(23753..24454)
                     /locus_tag="AC1PIW_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HIP6605079.1"
                     /translation="MLDISYQANTFHISYQGRPLGKIHSNQNPYHQTNIYLRLDLVDF
                     DCKIAKQLFQSLQTSLGGKPLQVMLSSAEQERIHFLTTAGFVCKRKCYEFEVTKQDYL
                     SQTGTSNLSIVLKGTAPYQIVCQQIFDHYQTVHQDINPWTASQEEFEKGLPDLVYTDS
                     ENCAFIDTDEIAYVCGKDEQSFGSFIQAVICQLFEQYEQISFEADDCDPIAMHLADQF
                     RQPNKPSWDAYILEI"
     gene            complement(24454..24792)
                     /locus_tag="AC1PIW_000104"
     CDS             complement(24454..24792)
                     /locus_tag="AC1PIW_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIP6605080.1"
                     /translation="MNFQDYIQDFTSVNVEQAQSLLTANEGAVLFIGRATCPYCNRFA
                     PKLHKVAQDKQVTIHFLDSSQVSPELQALRDHYQVPTVPGLLVAKSTGVQVRCDSSMT
                     EDEIAAFIQD"
     gene            24942..25400
                     /locus_tag="AC1PIW_000105"
     CDS             24942..25400
                     /locus_tag="AC1PIW_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HIP6605081.1"
                     /translation="MAMKNTSDYIEEHIKAILDQVSVAELRRSELASRFEVVPSQINY
                     VIKTRFTASRGYIVESKRGGGGYIRIGRITFSDKHELVHDLLKNMGDSLSATVFADIL
                     QLLFDEQLMTEREGNLLLATSTDEVLGNEASEIRARMMYQILRRLDRKEE"
     gene            25406..27859
                     /locus_tag="AC1PIW_000106"
     CDS             25406..27859
                     /locus_tag="AC1PIW_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIP6605082.1"
                     /translation="MKISRGLQGVYEDAQLIAQRYSSDYLETWHLLLAFVINPDTVAG
                     AILAEYPADVLDYERAVYMVMGRRYHEELESFFFLPSSKRVKELQVFAEKIAEIVKSK
                     GLGTEHIFMGMLLDKRSTASQILDQVGFHFEDSDDKVRFLDLRKNLEAKAGFTKEHLK
                     AIRTMTKGGKPKQATVGNMMGMTQSQSGGLEDYTRDLTALARSGQLEPVIGRDEEISR
                     MLQILSRKTKNNPVLVGDAGVGKTALALGLAQRIANGEVPASLVNMRILELDLMNVIA
                     GTRFRGDFEERMNNIINDIEEDGRVILFIDELHTIMGSGSGIDSILDAANILKPALSR
                     GTLRTVGATTQDEYQKHIEKDAALVRRFAKVTIEEPSVADSVAILQGLKPAYEAHHKV
                     TISDQAVVTAVAYAKRYLTSKNLPDSAIDLLDEASATVQNRAKGQVEEGGLTALDQAL
                     MAGKYKTVTQLLLKAQEAENQATSYSLEVTEEDILATLSRLSGIPVTKLSQTDAKKYL
                     NLEQELHKRVIGQEEAISAVSRAIRRNQSGIRTGHRPIGSFMFLGPTGVGKTELAKAL
                     AEILFDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLF
                     DEVEKAHPDIFNVLLQVLDDGVLTDRKGRKVDFSNTVIIMTSNLGATALRDDKTVGFG
                     ALDLSKSQEHVEKRIFEALKKAYRPEFINRIDEKVVFHSLTEADMQDVVKVMVKPLIA
                     VAASKGITLKLQASALKLLAKEGYDPEMGARPLRRLLQTKLEDPLAEMLLRGELPAGV
                     TLKVGVKAEQLKFDSVKAG"
     gene            27973..29010
                     /locus_tag="AC1PIW_000107"
     CDS             27973..29010
                     /locus_tag="AC1PIW_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIP6605083.1"
                     /translation="MKQAVAVIFGTFAPMHKGHIDLIQRAKRECDRAVVIVSGYKNDR
                     GHQIGLGLQKRFRYIRETFNDEPLVSVFKLDEEGMPPYPEGWAPWLEALQALVAIKEN
                     EELVFYVSEQEYAEELRSRGFKASFTERNFGISATLIREQSAKYWNMIAKPFRRHFSK
                     NILVVGSASNGKTTLVRDLGRYYSCPVSLEYARYYQQRYNVRDDELTGKDYNYLLTGQ
                     YRQTSDLIDSDTNRGLVIADTNATVTEAYYDYYIGETSSSFHSLCADTVKNEKWDLII
                     FVLPTGSYVDDGFRDMTMADAEIRNAFTEHLKELVAKNHPQASLAFIGGSYAENYAKA
                     IELIDAIYQEF"
     gene            29020..29841
                     /gene="pnuC"
                     /locus_tag="AC1PIW_000108"
     CDS             29020..29841
                     /gene="pnuC"
                     /locus_tag="AC1PIW_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HIP6605084.1"
                     /translation="MEQLTKFIDRFQASLKQVPENMSAIANRAKKLGVIGVLDAIIAG
                     LFFGRTLGQWLYLLVLSSVPVILHVWSASEDWMGLFTSWTGIICVILVAEGRSSNYFF
                     GFISNLIYFVLSYQNMFYGEVMTAIFFIVMQPVGLYFWLSARVNGAAEEEKTEFEARK
                     LTLWGWVKWLVFAVLVWGSFGLIYKSIGSARPFRDSITDGTNWTGQFLQSYLYREQWI
                     FWIATNVFSIYLWWVGPDSGNLQMSVMYFVWTINSLVGWYQWSKSIKEGQVARNG"
     gene            29834..30607
                     /locus_tag="AC1PIW_000109"
     CDS             29834..30607
                     /locus_tag="AC1PIW_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIP6605085.1"
                     /translation="MARPAGLSDKEYYEKYVTEAEFLDWYKKQDAPRFEKPSVTADMV
                     VYSFVEGRIKLLLIRRAAHPCQHKLSLVGGFIARGEDAYQTCQREIKKEAGLDLPRNH
                     IEQLLTVSDSERDPRGWMMTIAHLVYLPSQALDLVKPGPDGREPIVIDVDFKTSQCSY
                     EGQVLTAEDFAFDHYEIVMTSIQRIQGRMKWNPTFLNLLQQPFNIYAATDLVNLISPD
                     KKILHNNFLAKYGDFVEEVGVERLPKRKPRKTYRLKESS"
     gene            complement(30753..31757)
                     /gene="dusB"
                     /locus_tag="AC1PIW_000110"
     CDS             complement(30753..31757)
                     /gene="dusB"
                     /locus_tag="AC1PIW_000110"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HIP6605086.1"
                     /translation="MANLNTPFMIGDVEIPNRCVLAPMAGVTNSAFRTIAKEMGAGLV
                     VMEMISEKGLLYNNEKTLHMLHIDDNEYPMSIQLFGGDAEGLKRAADFIQKNTKANIV
                     DINMGCPVNKVIKNEAGAKWLKDPDKIYHIIKEVTSVLDIPLTVKMRTGWNNTDLAVE
                     NALAAESGGVAALAMHGRTREQMYTGTVDLETLTKVAGSLTKIPFIANGDIRNVEDAR
                     QRIEEVGADAVMVGRTAMGNPYIFNQINHYLETGEVLPDLSFDDKLNVAFDHLTRLTN
                     LKGESIAVREFRGLAPHYLRGSAGAAKIRGAVARAETIEQVQELFDQAREAYQERIAK
                     "
     gene            complement(31750..32616)
                     /gene="hslO"
                     /locus_tag="AC1PIW_000111"
     CDS             complement(31750..32616)
                     /gene="hslO"
                     /locus_tag="AC1PIW_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HIP6605087.1"
                     /translation="MDKIIKTLSKSGHFRAFVLDSTETVKTAQEKHDTMASSTVALGR
                     TLIANQILAVNEKGDTKITLKILASGAVGAIISVANTKGQVKGYIQNPDLDYKRTATG
                     EVIVGPLVGNGQFLVITDYGTGHPYNSMTPLISGEIGEDFAYFLTDSQQTPSAVGLNV
                     LLDEEDKVKVAGGFLLQVLPGATEVEIARFEKRIQEMPAISSLLASENHIEALLSAIY
                     GDDDFKRLSEEEIGFVCDCSKDRFLDALASLPKADLQEMKEEDKGVDITCQFCQTHYH
                     FDENDLEELING"
     gene            complement(32696..35815)
                     /locus_tag="AC1PIW_000112"
     CDS             complement(32696..35815)
                     /locus_tag="AC1PIW_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6359 domain-containing protein"
                     /protein_id="HIP6605088.1"
                     /translation="MKIRNRSLFYTVGSVAVTAGLLLSLATSPIPSVHATEVAIENYP
                     SLAITKSEVTIQGYLIAPLNSSGTAFDATNKTNLALGASMDTAAADTIPIQLKEPLRS
                     QFNLVNHPELVGKLVRITGTSDTYMKRAGIKPATAIEIVDSSSTNVQPPTSENTATKP
                     SDLVSTPIATVRSGAQGTEYTVSGKIISLVNGWGGNGFYLQGSDGAGIYIYPGAALGY
                     QLGDTVQLTGTLGEYKGELQLTTVSNHKAISENFNTPITETNIAQLATQAQATLVSLK
                     NLTVGDIQSDSYQNSTFTVTDSEGQTVDVRLDSRTGIKTADLLNRINKGDKINLTAIL
                     STYNGKIQLKPFDLSHFEVIEKATTEAGLGKTEAVTVGRIQGASHQSPLVNQSVMLKN
                     VVVTYVTSANNFYVQDVTPDGDTKTSDGINIFTDKLKTNVKVGDLVTIAGRVEEYQGR
                     GYAERDKTDLTITQIRATEVTVDGTAPVPSPIVLGLDRTIPADIIDNDGLAQFDPEQD
                     ALDFWESVEGMVVAVDDAKILGPLKNKEIYVTPATSQLPLNNVGGVNLRPEGNNTNII
                     PLLLKNGKQIVKSGDYFIGRIAGPVTYSYTNYKVYVDDSTLPTLHEGATKPETTTIIP
                     NDDKLTIASYNIENFSANSKSTSDAKVQRIAKSFVSDLHSPDVIGLIEVQDNNGATND
                     GTTDASKSAERLIAAIQAAGGPTYTYVDIAPENNKDGGQEGGNIRVGFLYNSKRVSLS
                     DKPIGTATQAVAWENGELNLSLGRIDPTNPAWAAVRKTLAAEFVFKGEKVVVLANHLN
                     SKRGDNGLYGKIQPVSFKSEEKRHILAQTIADFTKAGLAQNPNANIVMLGDFNDYEFT
                     KTIEILEAGGMANLVSRHDASDRFSYFYNGNNQSLDNMLVSTNLLERYAFDMVHVNSA
                     FMEEHGRASDHDPLLVQLDVTKAQEPTQPEPSDKQTDDSGTVNNSDDNGTTNNNKPTN
                     LSTSNQTAVNADDRSGATDKRQTTVTPTANNSQKKILPKTGGETSFVLITIGLVFLSA
                     CLVKKQKES"
     gene            complement(36701..38932)
                     /gene="gshAB"
                     /locus_tag="AC1PIW_000113"
     CDS             complement(36701..38932)
                     /gene="gshAB"
                     /locus_tag="AC1PIW_000113"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HIP6605089.1"
                     /translation="MLQKLSPNSPILQATFGIERESLRINSNHRVAQTPHPHKLGSRS
                     FHPYIQTDYSEPQLELITPIAQSTKEARRLLGAITDVAARSMDKQEYLWPLSMPPVIS
                     EEEIQIAQLDSDYEYQYRVGLGERYGKLVQSMSGIHYNFELGKDLTQQLFELSEETDF
                     IAFKNTLYLKLAQNFLNYRWLLTYLYGASSLAEKGFLTTEVGCVRSIRNSKYGYVNSD
                     DVHISFSSLQQYVADIEQAVQSGQLSAEKEFYSSVRLRGAKTSRDYLSKGISYLEFRS
                     FDLNPYDPLAISQETLDTVHLFILSLLWLDQLTDVDNTLAKADKLNNLIALSHPHTPL
                     PNDADATPILTAMKAIVLHFGLDDYYGQLIAHAEAALQDPRLTLSGKIAEQVEDGSLE
                     KFGQQQGQVFHDYAWTAPYALKGYENMELSTQMILFDAIQLGLNVEILDEEDQFLKLW
                     HGNHVEYIKNGNMTSKDNYVIPLAMANKVVTKKILDQAGFPVPAGAEFANKTDALRYY
                     GQVASSAIVIKPKSTNFGLGISIFQEPASLADYEKALDIAFSEDSHVLVEEFVAGTEY
                     RFFILDGKCEAVLLRVAANVVGDGSSTIRELVEQKNQDPLRGRDHRSPLEIINLGDIE
                     LLMLEQQGYTPDTVLPEGIQAFLRGNSNISTGGDSIDMTDQMDESYKQLAADMATAMG
                     AWACGVDLIIPDRTKPASKNKPNCTCIELNFNPAMYLHTYTYAGPGQSITPKILKKLF
                     PEL"
     gene            39297..40589
                     /locus_tag="AC1PIW_000114"
     CDS             39297..40589
                     /locus_tag="AC1PIW_000114"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HIP6605090.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGTKYKLHLIPSGIFFPEKISVIGNGVVVNPKSLVKEINYLHDSGVTTDNLRISDRA
                     HVILPYHIKLDQLQEESKGENKIGTTNKGIGPAYMDKAARVGIRIADLLDKEIFAERL
                     RTNLAEKNRLFEKMYESTPIEFDEIFEEYYAYGQEIKKYVTDTSVILNDALDQGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELHDEIGDRIREIGKEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLGTLKICVAYDLDGERIDHYPASLEQLKRCKPIYEEMPGWSEDITGVRSLDELPEA
                     ARNYVRRISELVGVRISTFSVGPGREQTNILESVWSAK"
     gene            40699..41022
                     /gene="yajC"
                     /locus_tag="AC1PIW_000115"
     CDS             40699..41022
                     /gene="yajC"
                     /locus_tag="AC1PIW_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HIP6605091.1"
                     /translation="MEGLFLPLVMVAMIGFMFYSQRKQQKQRQEALNQIKKGDEIVTI
                     GGLFGIVDEIDDKKVVLDVDGVYLTFERGAIRDRVASSDATSAVVEEVAVETPEATPE
                     SAIEE"
     gene            41136..41891
                     /locus_tag="AC1PIW_000116"
     CDS             41136..41891
                     /locus_tag="AC1PIW_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HIP6605092.1"
                     /translation="MFKFNFKKKESIETAIEVPKHIAVIMDGNGRWAKKRMQPRIMGH
                     KAGMDALQKVTKTASDLGVKVLTVYAFSTENWSRPEKEVKFIMNLPVEFYDKYVPELH
                     ANNVKIQMIGDHSKLPVPTLNALYKAEQKTKGNTGLILNFALNYGGRDEVTRAVKAIA
                     QDVLDAKFNPGDIDEKLIADYLYTGTLSPTLRDPDLVIRTSGELRLSNFLPWQTAYSE
                     LYFTDIAWPDFDGEALKLAIKEYNRRHRRFGGV"
     gene            41901..42707
                     /locus_tag="AC1PIW_000117"
     CDS             41901..42707
                     /locus_tag="AC1PIW_000117"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIP6605093.1"
                     /translation="MTNDLQKRVIFGGIALAIFIPFLLAGGMAFQFFVGLLAIIATAE
                     LIKMYRLAPNSIEGVLAMLASLVLALPLQNYLTFLPTDGNYTAYAIVVFLLLGSTVFN
                     IGHYNYSDVVFPIASSFYVGIGFHSLILARMDGLNKVFFALCLVWATDIGAYLIGRQI
                     GRRKLLPKVSPNKTVEGFVGGILSAVVVAIIFLIVDKSLLAGYSFGMMLLLVVIFSIF
                     SQFGDLVESAIKRHFGVKDSGKIIPGHGGILDRFDGMIFVFPIMHFFGLF"
     gene            42717..43976
                     /locus_tag="AC1PIW_000118"
     CDS             42717..43976
                     /locus_tag="AC1PIW_000118"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HIP6605094.1"
                     /translation="MKGILAFIFIFGVIVVVHEFGHFYFAKKAGILVREFAIGMGPKI
                     FAHTGKDGTLYTIRILPLGGYVRMAGWGEDKTEIKTGSPASLSLNEAGVVTRINLSGK
                     QLDSLSLPMNVTSFDFEEKLEITGLVLEESKTYKVDHDATIVEEDGTEVRIAPLDVQY
                     QNATVWGRLMTNFAGPMNNFILGILVFILLFFMQGGVANPSSNAVSITEGGALQAAGV
                     VTGDKILSVNGNTTDSYTEVATIISKAATDATTAPSFDLVVEHDGKNRHVSVTAEQVD
                     GAYRIGISPILKTGFVDKIVGGFQEAGATALRVVTALKNLIANFDVKQLGGPVAIYKV
                     SSQAAEFGLVSVLGLMAALSINLGIFNLIPIPALDGGKIVMNILEAIRRKPLKPETES
                     YITLAGVAVMVVLMIVVTWNDIIRVFF"
     gene            44257..46119
                     /locus_tag="AC1PIW_000119"
     CDS             44257..46119
                     /locus_tag="AC1PIW_000119"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIP6605095.1"
                     /translation="MKQSKMIIPTLREMPSDASVISHALMLRAGYVRQISAGIYSYLP
                     LANRVIEKAKNIMREEFDKIDAIEFLAPALLSADIWRESGRYETYGDDLYKLKNREGS
                     DFILGPTHEETVTLLARDAVQSYKQLPLNIYQIQPKYRDEKRPRNGLLRGREFIMKDG
                     YSFHASYESLDQTYDDYKAAYEAIFTRAGLEFKAIIGDGGAMGGKDSQEFMAITPDRT
                     DLDRWVVLDKSVASFEEIPEDVLEAFKAELLAWSVSGEDTIAYSSESGYAANLEMATS
                     EYKPSTAVVVEEDLVKVATPDAKTIDEVAAFLNVAEEQTIKTMLFMADGEPVVALLVG
                     NDQVNDVKLKNHLAADFFDVASPADAEKVFGAGFGSLGPVGLPENIKIIADRKVQDVK
                     NAVVGANEDGFHYTGANAGRDFQVTEYVDIREVKEGEPSPDGHGVLNFARGIEIGHIF
                     KLGTRYSDSMNANILDENGRSMPIIMGCYGIGVSRLLSAVLEQHARLFVNKTPKGEYR
                     YAWGINFPKELAPFDVHLIPVNVKDEAAMELTQSIEASLVGAGYEVLTDDRNERVGVK
                     FSDSDLIGLPIRVTVGKKAADGIVEVKIKGTGDTVEVHVDQLLETLQILTKENE"
     gene            complement(46301..47011)
                     /locus_tag="AC1PIW_000120"
     CDS             complement(46301..47011)
                     /locus_tag="AC1PIW_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HIP6605096.1"
                     /translation="MNLIGELLGTFILVLLGNGVVASCILSKTKAENSGWLTIVLGWG
                     IAVTIAVYISGIFGPAHLNPAVTIAMASIGSLPWAQVPGFLLAQFAGAMLASIVLYLH
                     FYPHWQETKDSGTILATFSTAPALRHTASNLLGEALGTAVLVMGILAIGPNNVSAGLG
                     PIIVGLIVFAIGFSLGSTTGYAVNPARDLGPRIMHAILPIPNKGDSDWSYSWIPVAGP
                     ILGGVAGAILYNILLNML"
     gene            complement(47021..47395)
                     /gene="dhaM"
                     /locus_tag="AC1PIW_000121"
     CDS             complement(47021..47395)
                     /gene="dhaM"
                     /locus_tag="AC1PIW_000121"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HIP6605097.1"
                     /translation="MTKVGILLVSHSKNLAQGIIDLVSEVAKDIPITYCGGLEDGSIG
                     TSFEIVQERVEANPADTLLAFFDLGSARMNMELAADFTDKQILIQTVPVVEGCYTAAA
                     LLQAGADLETILEQLKELTINK"
     gene            complement(47392..47967)
                     /gene="dhaL"
                     /locus_tag="AC1PIW_000122"
     CDS             complement(47392..47967)
                     /gene="dhaL"
                     /locus_tag="AC1PIW_000122"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HIP6605098.1"
                     /translation="MDAKQALLWMETFNKKIQENKDYLSELDSPIGDGDHGGNMARGM
                     AAVMQELSEKDYETADQVFKVVAMQLLSKVGGASGPLYGSAFMGITKASQAGADLADT
                     LQAGLDMIQKRGKAELGEKTMVDVWIPVISALRESQLTTEIIHKAVQGTKDIAATKGR
                     ASYVGERSIGHIDPGSFSSGLLFEAMLEAGL"
     gene            complement(47978..48967)
                     /gene="dhaK"
                     /locus_tag="AC1PIW_000123"
     CDS             complement(47978..48967)
                     /gene="dhaK"
                     /locus_tag="AC1PIW_000123"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HIP6605099.1"
                     /translation="MKKIINQPEHVVDEMLKGLVFMHQDLVDRIDGFDVIIRKAEKTG
                     KVGIISGGGSGHEPSHAGFVGKGMLSAAVCGAVFTSPTPDQILEAIKAADEGAGVFMV
                     IKNYSGDIMNFEMAQELAEMEGIEVASVIVDDDIAVENSLYTQGRRGVAGTILVHKIL
                     GHAATTGKSLAEIKELADRLVPQIKTIGLALSGATVPEVGKPGFVLEEDEFEYGVGIH
                     GEPGYKKEKLQPSAKLAEELVEKLAEGFDIQDGEHYGVLINGLGATPLMEQYVFANDV
                     AQLLAKKGVVVDYKKIGNYMTSIDMAGLSLTLIKLEDPSWIDALQAPVEVVAW"
     gene            49120..49665
                     /gene="dhaS"
                     /locus_tag="AC1PIW_000124"
     CDS             49120..49665
                     /gene="dhaS"
                     /locus_tag="AC1PIW_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase transcriptional
                     activator DhaS"
                     /protein_id="HIP6605100.1"
                     /translation="MPTSLITKKRIAKAFKKQLSQKSFDKISVVDIMQEAGIRRQTFY
                     NHFLDKYELLDWIFETELQEQVTDNLTYISGIKLLDELLYYIESNQSFYRQLFEIKGQ
                     NDFVSYLEGYFMVLMEKIINEYQLQEGKMISQQDKHFLILYHANAWIGLIKEGLTQSP
                     CQIHSYWKQMVALVRESFVIL"
     gene            49676..50665
                     /gene="dhaQ"
                     /locus_tag="AC1PIW_000125"
     CDS             49676..50665
                     /gene="dhaQ"
                     /locus_tag="AC1PIW_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DhaKLM operon coactivator DhaQ"
                     /protein_id="HIP6605101.1"
                     /translation="MVFIRNRQATIIEDYLDGFCRTNPKVKKVKGLPIVLQKVQDDHL
                     VPIISGGGSGHEPAHIGFVGKGMLTAAVYGEIFTPPTAEEVFAAIRAVDKGKGVFLIV
                     KNFEADLISFRKAIELARQEGIAVKYIVSHDDISVDTKNNFRMRHRGLAGTILLHKIL
                     GAAALTGYSLDELEQLGLSLATEIATIGFATKSAYLPNAALPLFDLEEGQISYGIGIH
                     GEEGYRTVRFESSEQLANEIVNKLKLKFRWKEGEDYILLVNNLGSMSEVEQGIFLNDI
                     CQFLELEGLQLSYVKTGKFMTSLDMGGISVTLCRVKDRKWLDFLEAPTEAAAW"
     gene            50790..55181
                     /locus_tag="AC1PIW_000126"
     CDS             50790..55181
                     /locus_tag="AC1PIW_000126"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002907973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HIP6605102.1"
                     /translation="MSDKFQLLLQQIGMPLDARQSGAFSTATIEKVVLHKVSKLWEFT
                     FRFETPLPLMDYQLFKARLATEFEKVGNKIQFSIVSDAEAFEAGLVEAYYPEAFTEDL
                     CQSAGFKALFQPLEVAYRDGVLWIKGPETIDTDHFRKNHLPNLVEQYKRFGFGNLAVD
                     IQVCQEMTQQQAEIFHAQNAEIYQQANEENLAALEQLAQMAPPPEAAQPFVPEYKKNR
                     PAKVNIEKAEITPMIEVDSEENRIVFEGLVFEVEQKTTKTGRVIINFKMTDYTSSFTL
                     QKWAKNEEEAQKFDMVKKGNWLRVRGNVETNNFTRDLTMNVQEVQEVKKEIRKDLMPE
                     GEKRVEFHAHTNMSTMDALPAVEDLVARAAAWGHKAVAITDHGNVQSFPHGYHAARKA
                     GIKPLFGMEANIVEDSVPIAYNEADVVLSDATYVVFDVETTGLSAVNNALIQIAASKM
                     HKGNIIAEFDEFIDPGHPLSQFTTDLTGITDEHVRGSKPLEQVLREFQDFCQDSVMVA
                     HNATFDVGFMNVNYERAGLPIISQPVIDTLEFARNLYPDFKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFLKDALEKHNLTNLNQLNTELIAEDSYKKARVKHATLYVIN
                     QVGLKNMFKLVSLSNTKYFEGVPRIPRTVLNAHREGLILGTACQEGEVFDDLLSKGID
                     EAVKTAAYYDFIEVMPPALYAPMIAKEQFKDMAEIEETIKQLIEVGRRAGLPVLATGN
                     VHYIDPEEEIYREIIVRALGQGAPINWTIGNGENAQPAPLPKAHFRTTSEMLDEFAFL
                     GESLAREIVITNPNAMLDRFEDVQVVKTDLYTPYIEKAEETVAELTYQKAFEIYGNPL
                     PDIIDLRIEKELTSILGNGFAVIYLASQMLVHRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPMPPHYVCPNCQHSEFITDGSYGSGFDLPDKDCEKCGTKYKKDGQDIPFETFLG
                     FDGDKVPDIDLNFSGDDQPSAHLDVRDIFGEENAFRAGTVGTVAAKTAYGFVRGYERD
                     YGKFYRDVEVERLAAGAAGVKRTTGQHPGGIIVFPDYMDVYDFTPVQYPADDVTASWQ
                     TTHFNFHDIDENVLKLDILGHDDPTMVRKLQDLSGIDPQTIPADDKGVMALFSGTEIL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVEETKPTTFSELLQLSGLSHGTDVWLGNAQD
                     LIKAGIANLSTVIGCRDDIMVYLMHAGLPPKMAFNIMERVRKGLWLKISEEERNGYIQ
                     AMKDNKVPDWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPLYYYCAYFSIRAK
                     AFDLATMSGGLERVKAKMEEIALKKKNNEASNVEQDLYTTLELVNEMLERGFKFGKLD
                     LYKSHATDFLIEEDTLIPPFVAMDGLGENVAKQVVAARAEGEFLSKTELRKRGGLSGT
                     LVEKMDEMGILGKMPEDNQLSLFDDLF"
     gene            55193..55864
                     /locus_tag="AC1PIW_000127"
     CDS             55193..55864
                     /locus_tag="AC1PIW_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIP6605103.1"
                     /translation="MKKRNILVELLKGIGLVLLSTILWTLILSFATPESSSGIYEPFP
                     GFTVIAGILTSFILHIIMKYNQLRQAEQAAKASKSHIVVIEERNQALMKKANRLVEKH
                     QNLEKETILDINKSVSKIRTVEEKTVMKSTKIESSQEFGQFLRQMPELMANKHIERLL
                     NEIFETENNLAQWKISYNQQVEYFNTLLHQFPISVIAKIIRLKDLEFYKEHSLTDFTD
                     EMLGL"
     gene            56100..56702
                     /locus_tag="AC1PIW_000128"
     CDS             56100..56702
                     /locus_tag="AC1PIW_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIP6605104.1"
                     /translation="MSNFAKLQETRRSIYALGKDLPVSNEEVVALVEKAMKESPSAFN
                     SQSSRAVVLFGAESEVFWNEIAYSELEKVTPAEAFEATKGRLAGFAAGAGTILFFEDQ
                     DVVKGLQESFPLYAENFPIWFEQAHGINLYAVWLAFAEKNIGMNVQHYNPLVDAQVAE
                     KYGIPANWKLRAQAPFGSIAAPAADKDYMADSDRVKVFGN"
     gene            56975..58216
                     /locus_tag="AC1PIW_000129"
     CDS             56975..58216
                     /locus_tag="AC1PIW_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIP6605105.1"
                     /translation="MVLPNFKENLAKYAKLLVSTGINVQPGHTVQLTIGVEQAELARL
                     IVKEAYAHGAKEVLVNWLDDVIARERLVNVDVELLEQVHPQRITEMNYLLERKASRLV
                     VLSEDPGAYDGVDPEKLSRNARALSQALNPMRQASQANKISWTLGAASGLEWAKKVFP
                     NATSDEEAVDLLWDQIFKTCRIYDEDPIKAWEEHEARLVAKAKVLNDEQFVKLHYTAP
                     GTDLVLGMPKNHLWEAAGSINAQGEKFIANMPTEEVFTAPDYRVADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGEIVDVTAEKGDEVMKKLVFDNAGARGLGEVALVPDKSPISQSGV
                     TFFNTLFDENASNHLAIGQAYAFSIEGGTEMSQEELKEAGLNRSDVHVDFMIGSNKMN
                     IDGIREDGTRVPIFRDGEWAI"
     gene            58275..58508
                     /locus_tag="AC1PIW_000130"
     CDS             58275..58508
                     /locus_tag="AC1PIW_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HIP6605106.1"
                     /translation="MLSSIIAGAIIGLIAGALTSSDDRRGCLGNIILGLAGSWIGQLL
                     FGDWGLQFAGMAVVPSVLGAILLVAIFSSSRRN"
     gene            58888..60450
                     /locus_tag="AC1PIW_000131"
     CDS             58888..60450
                     /locus_tag="AC1PIW_000131"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIP6605107.1"
                     /translation="MRVIEFLDTTLRDGEQTPGVNFSIKEKVTIAKQLEKWGISAIEA
                     GFPAASPDSFEAVRQIAAAMTKTAVTGLARSVKSDIDACYEALKDAKYPQIHVFIATS
                     PIHREFKLQKTKEEILAQITEHVSYARERFEVVEFSPEDATRTELDYLLEVVQTAVDA
                     GATYINIPDTVGFTTPQHYGEIFRYLTTNVKSDREIIFSPHCHNDLGMAVANTLSAIK
                     NGAGRVEGTINGIGERAGNAALEEVAVALEIRKDFYDVTSPIVLKETLNTSELVSRFS
                     GIAIPRNKAVVGGNAFSHESGIHQDGVLKNPSTYEIITPELVGVKKNSLPLGKLSGRH
                     AFVEKLKELDLYFEEGDVASLFARFKNLADKKEKITDADIRALVAGTEISNRDGFQFK
                     DLRLDSQSDGSIQAQVIFINQDEEEVVVSESGKGSVEAVFNAIDQFFHQEISLERYHI
                     DAITDGIDAQARVLVAVENKATETVFNASGIDFDVVKASAIAYIHANVMVQKENSGQI
                     DKKLSEKELPHI"
     gene            60459..61505
                     /gene="leuB"
                     /locus_tag="AC1PIW_000132"
     CDS             60459..61505
                     /gene="leuB"
                     /locus_tag="AC1PIW_000132"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIP6605108.1"
                     /translation="MTKKIVALAGDGIGPEIMEAGLAVLEAVAGQVGFDYEIEERAFG
                     GAGIDASGHPLPKETLQACRKADAILLAAIGSPQYDDAAVRPEQGLLQLRKELGLFAN
                     IRPVKIFDSLKDYSPLKVDRLDGVDLVMVRELTGGIYFGEHILGTDQASDSNTYQAEE
                     IERVVRSAFDLAQKRRKKVTSIDKQNVLATSKLWRKVADEVAKDYPDVVLEHQLVDSA
                     AMLLITNPSRFDVLVTENLFGDILSDEASVLTGTLGVLPSASHAVAGPSLYEPIHGSA
                     PDIAGQGIANPVSMILSVAMMLEESFDLVQAGQTIRQAVEAVFAEGIFTKDLGGSAST
                     KEMTAAIIAQIGGG"
     gene            61508..62827
                     /gene="leuC"
                     /locus_tag="AC1PIW_000133"
     CDS             61508..62827
                     /gene="leuC"
                     /locus_tag="AC1PIW_000133"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIP6605109.1"
                     /translation="MSGKSIFDKLWDRHVITGQEGQPQLLYVDQHYIHEVTSPQAFQG
                     LRDTGRSVRRPDLTFATFDHNVPTVDIHNIRDVISKAQMDALAKNIEDFGIPHAAHGS
                     EHQGIVHMVGPETGRTQPGKFIVCGDSHTATHGAFGAIAFGIGTSEVEHVLATQTIWQ
                     VKPKRLLVEFVGQAQRGVYAKDFILALIARYGVDCGLGHVVEFAGPVIDRLSMEERMT
                     ICNMSIEFGSKMGIMAPDQTTFDYLRGRECAPADFDAAVADWKELYSDADTVYDKHLV
                     LDVSDLAPMVTWGTTPAMGVSFDETFPSIRDHNDERVYAYMDLAPGQKAADIPVGYVF
                     LGSCTNARLSDLQLAARIVKGRKIAEQVTAIVVPGSRPVKRAAEKLGLDKVPAGVHCA
                     STSNRNFEDRQGFGAKTHLCSPAMAATAALYGRFVDTRQLDILKAGV"
     gene            62827..63420
                     /gene="leuD"
                     /locus_tag="AC1PIW_000134"
     CDS             62827..63420
                     /gene="leuD"
                     /locus_tag="AC1PIW_000134"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIP6605110.1"
                     /translation="MHQFTTWTGTTVPLMNDNIDTDQLLPKQFLKLIDKKGFGKYLLY
                     AWRYLDDNYTDNPDFILNQPEYQGASILISGDNFGAGSSREHAAWALADYGFKVIIAG
                     SFGDIHYNNDLNNGILPIIQPKEVRDQLAQLGPDQEITVDLADQLIRTPFGECPFDIE
                     QDWKHKLLNGLDDIGITLQYQDLIAEYEDNRPSYWQN"
     gene            63480..64265
                     /locus_tag="AC1PIW_000135"
     CDS             63480..64265
                     /locus_tag="AC1PIW_000135"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIP6605111.1"
                     /translation="MTKQIHWDGGLSQEGFDIIKGEGGVIVCPTKVGYIIMTADKAGL
                     ERKFDAKERKRNKPGVVLCGSMEELRELAQLTPEIDAFYQKHWDEDILLGCILPWKPE
                     AYAKLQAYGDGREELMTDVRGTSCFVIKFGVAGEQIAKEMWEKEGRMVYASSANPSGK
                     GNRGKVEGIGERIASKVDLIIEADDYVTSIQPDKTIETRYEQGVMVSMVDKDGKLIPE
                     QSADSRSISPCPVVIRKGLDIDKIMMHLSDHFNSWDYRQGEYY"
     gene            complement(64454..65068)
                     /gene="def"
                     /locus_tag="AC1PIW_000136"
     CDS             complement(64454..65068)
                     /gene="def"
                     /locus_tag="AC1PIW_000136"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIP6605112.1"
                     /translation="MSVIERLTKAAHLIDMDDIIREGHPTLRQVAEEVAFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMKLRGGVGLAAPQIDVSKRIIAVLVPNPEDEEGNPPAQ
                     AYSLQEVLYNPKIVAHSVQEAAMEGGEGCLSVDREVQGYVVRHARVTVDYMDKNGEKH
                     RIKLKGFNAIVVQHEIDHLNGVMFYDRIDPEHPFAVKEGMLVIE"
     gene            65198..66367
                     /locus_tag="AC1PIW_000137"
     CDS             65198..66367
                     /locus_tag="AC1PIW_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIP6605113.1"
                     /translation="MKRILEKASLLALSTMLVSTFAVSPAIPQMIEYFAQQGIAAAQV
                     ENLITVTSFAIMVTLLANGLVVRFLSERNIIIAGLLLMAIGGALPMFLTAYPLILLAR
                     ILLGLGIGLINARAINIIGNFFTGQERVQMMGLRGSAEVLGSAGLTLLVGWLTQFGWQ
                     PAFLVYLFALVILALFLIFVPKEELLSRMEVKVEENAPKVKLDKTMWMMGIYLAFLAF
                     FVINVNTFLTIRIPQIVLAKGIGTAQQASLILSLMQVMGIVAGTVFSSLVGRLKGWLL
                     AVSYVVFGLAVVGIAFADNLWVLGLGGMVSGFFYSIILTIVFSQVTDRAPKALLNAVM
                     TIVLMGCNIGGATSAILPTYLEKLNPTPTGAFGIYAIGCALISAGLIYKQLKKWE"
     gene            66664..66837
                     /locus_tag="AC1PIW_000138"
     CDS             66664..66837
                     /locus_tag="AC1PIW_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HIP6605114.1"
                     /translation="MLENNRLNELILRFPEDVQLIFKDMIPSYQLGYVDYVYQTDSYA
                     TQNRRIKNLAKNF"
     gene            66841..67290
                     /locus_tag="AC1PIW_000139"
     CDS             66841..67290
                     /locus_tag="AC1PIW_000139"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6605115.1"
                     /translation="MDYFYIMPLPQDLALEIANQWRYQPPLDAYTISANPEIYAVMIS
                     PEARGDRFFAVIRNAALMGYFCIDQDGETVDIRLGMKPSLTGQGNGRAFYQAIEDYLV
                     EHVQPASIKLTVASSHQVAQKLFHALGFTEIEKQAEYIKMEKKLVCD"
     gene            67281..68009
                     /locus_tag="AC1PIW_000140"
     CDS             67281..68009
                     /locus_tag="AC1PIW_000140"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIP6605116.1"
                     /translation="MRLDKFLVDCGVGSRTEVKKLLKNKQVTVNGQVETSPKQQINEK
                     KDQIAVAGQVLSHETFVYYLLNKPKGVISATEDDHHRTVLDLLDNTARQKEVFPVGRL
                     DIDTHGLLLLTNKGQLAHAMLSPKKHVDKLYRAQVDGIMTQEDVERFAAGIELKDFTC
                     QPAQLTILSTDEAKQTSLVDITIREGKFHQIKRMVQACGKTVTDLQRLTMGPLRLDPK
                     LALGEYRRLTAAELKQLEVFGVEL"
     gene            68411..>68914
                     /locus_tag="AC1PIW_000141"
     CDS             68411..>68914
                     /locus_tag="AC1PIW_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605117.1"
                     /translation="MLKYIRALPLVIVAGAVVYFTIFICIDNGDWTYVVTRALGLKEE
                     HANNVSLIVIGAQSLFLTSDWFYKRIRAFLNRRLEKAKGLNRQIYLGYKAGIEFLMAL
                     PKRTILFTFYLLLVVAESVGLIEKAKDYAAVIIFIIAVDRVTKIWPEEKGRMIEFSQK
                     IKKRIKSV"
BASE COUNT        19075 a        13736 c        16887 g        19216 t
ORIGIN      
        1 ggaaaataat tatagaatta acgaataaat gtcatttaca gtgtacacac tgttttaagg
       61 aagctggacc aattaacagg aattttttga agtatgaaga gctaataaat tttttagata
      121 gggtaaaagg aaaggtttat gaaatacagt taacgggtgg ggagccgatg gcacatcctc
      181 attttaaaga gatttcaaaa tatgctacgg agaactttca agaagtaaca atgacaacca
      241 ctggtcattt aattaattcc aaaagtataa tttatttgaa gggaatgagc tacattcaaa
      301 tttctttata tcatcataat ccgattctaa acgaccaaat taccaatgga aaggatacat
      361 taaataaaac gattagagga ataagatttc taaacaatga gaatttagac tattcagtga
      421 ccaatattgt tcgaaacagt cttatcgacg aatttgataa ttttatagaa tttttaatac
      481 taaaccaagt aaaagcggtg agatttgggc tatttagtca tttgggtaga ggtaagttag
      541 tcggatcaga ttggttttta agtccctcta aagtagaaga attttatgag atgcttatag
      601 aaaaaagtag agagtatggg agcagaatta atatacatac atgggaagaa gataattgct
      661 ttaactattt tgaagacatt gtagatggga caatgcgttg cggagcaggt gttattgaat
      721 ggacaatcaa tgagagtggc catattaagc cgtgtacatt ctttccggat ggtgagtttt
      781 cttcttactc tcttcaaaat tttgaagaat attcaatgca aaatcacgaa cagaatattg
      841 taaggaaaat aaatgattgg gaaggtttgc tccagacggt tgggttatca actagaaata
      901 tttgcgaaga aatctataaa atggtggaga agaaatgaaa aagaggaatt tttggctaac
      961 cttatcttgt atttttgcag tagccctgct aaatgttggc tttctgtacc aatatcgcta
     1021 tattattgat gctattgcag ctggagatag acaggccttt gtgtcttatt ttgtgcagat
     1081 ggggttaact gtccttgtca tgctggtttt tgaatacatt cgtcagattg ccaatgtcag
     1141 ctatctcaat caagtaggtt tccaattgca ggcgaccttt attgggaaaa tttttgattt
     1201 accttttagg cagtttgttg agcagggggc aggggcctat atttctcagc tcaataatga
     1261 tttggagacg gtgaaagaag attattacga cgctttcttt acaatttttc aaggtgcttg
     1321 tacctttggc attgccagtt tggcccttct tagcttggat ggtctgaccg ctattttctt
     1381 gattctgatc tcattcttac cagttgtgat tccctatttg tttaaaaagc ggagaaggct
     1441 caatcaggag gcgatttcgg acagtcagca ggtctataat acaagagttt cagatgtctt
     1501 tctgagttat ttgcaagtga aaaatagcca ccaaagacag caagttttag atggtctgaa
     1561 cgatcgctat caagcagtca atgacaaggt caatcaggcc actcgaacga cagtaaccat
     1621 gcggctctta gtcggttttg tcttttatct gaccactctt tcgattatct ttatcggtgg
     1681 ttttcaagtc ttttctggtg ccttgaccat cggtggcttg acagctattc tgaccatttc
     1741 agagcagttg gtggatccca ttaatagcat tgcggcagcc tttttggaca ggcatgccgt
     1801 taagaaatta aagcaagagt ttgagctatc tacttttggt caagaaacga agggacagga
     1861 actagcttca gctttccaga gtattcaact ggtcaaggct agttatgctg tcggtgaaaa
     1921 acaggtcttt gaagacctga ccgtagcgtt tgagcgtggc aagaagtatt taattcttgg
     1981 ggaaagtggt tctggcaaat cttccttggc tcttattttg accaagaata gccaactgca
     2041 agcaggggat atttacgttg atcaaatctc tctttcagac ttatcctacc aagccattca
     2101 agacaagatt gcctatctgc cacaagaggg atcccttttc catgatacgg ttctatataa
     2161 cttgaccatg ggacgggagg ttccagaaga taggctgatg tttcacatta agaccgtgaa
     2221 ccttgacagt cgtttcccaa gttatgcttc tttgagcgag gaaattagtg atgatagcgg
     2281 actgtctggt ggacaaaagc aacgcctgct cttgatccgt gccttgctgc aagataagga
     2341 tatcttgttg ctggatgaaa gtctgtctgc cttggatcag gaaacctatg cagtgattga
     2401 aaattacctt gcctcactag ctgataagac gctcattcat atttcccacc gcgtgtccga
     2461 tgaggtcctt agccgttatg atggggtggt acggattggg gagagcaatt aatactcttc
     2521 gaaaatcaaa atcagacgtt gttgacttga tttgatgagc gaggctgggc aaaaagcaca
     2581 gcaccgcttc acaaaattag tgtcaacatc tcagcgcagt gattgattgg cagatttgtt
     2641 cgtgttttac actccaaatc tgacctaatc aactgtgcgg ggggtgggaa gacgaactct
     2701 tctatgaatg gtcgagttct ttcccactcc cagcctgttc ctttgaaaca aaatagtaac
     2761 agggttctat ctgcgtaagt agaaacgaac tacgttcgct ctatttccaa cctccaaagg
     2821 ttcccaaaac ctttggagct agtctgtttt tgattttcat tgagtatgag tgagaagata
     2881 taaaaatgcc cccaagtcat caactttgac ttggggtctc tccatttatc atgcggagga
     2941 agggatttga accctcacac ccgatgggca catgcgcctg aagcatgcgt gtctgccgtt
     3001 ccaccacctc cgcttgcttt ttattatact attttttatt tgaaatgact agagtaaagt
     3061 gtaaaatctc agagtctggg taaaagacca tctcacttca taaggttagt gtcaacatct
     3121 cagcgcagtg gttgattggt agaatcgttc gtgttttaca ctccaaatct aaccattacg
     3181 actgttgcga acaaagttcg ctttatatcc aacctcaaac tgtctcccag acagtttgag
     3241 ctgtgcgggg gtgggattga aacagtctgg gaatagactg tttcagctca acaacttgaa
     3301 acgtggaacg ggtgacgcgc tcttttatta aggttcgagt tctttcctgc tccttttttc
     3361 ttaggaaatt tggtataata ggaacaagta cacacaaagg agtcttcatt gcaaggaaga
     3421 attatcaagg ccttggcagg tttttactat gtcgaggcgg atggacagat ttatcaaacc
     3481 agagctagag gaaattttcg taagaaggga caaacgccct acgtgggtga ttttgtggac
     3541 ttttctgcgg aggaaaactc agaaggctat atattaaaga ttcatgagcg gaaaaatagt
     3601 ttggttcgac ctcctatcgt caatatcgat caggcggtgg ttatcatgtc ggccaaggaa
     3661 cctgatttta atgccaatct actggatcgt ttcctagttc tccttgaaca gaaggatatg
     3721 gatcccatta tctatatctc taagatggac ttggtagaag atcggacgga gatggatgcc
     3781 ttcaaggcta tctatgagaa aattggctat ccttttgtct atagtttgga ggaattgatg
     3841 cctcttttac aggacaaggt gacggtcttt atggggcaga caggggttgg gaaatcgact
     3901 cttctcaatc gcattgcgcc agaattagcc ttggaaacag gggcgatttc agatagtctg
     3961 gggcgtggtc gccataccac tcgtgcggtc agcttctaca atgtcttcgg tggaaaaatt
     4021 gcggatacac ccggtttttc atccctagac tatgaagtca aggaggcgga ggctctgaca
     4081 gattgtttcc cagagattgc ggaaagtagc caggattgca aatttaggac ctgtacccat
     4141 acccatgagc cggattgtgc ggtcaaggaa gctgttgcca gctctgccat ttcccaaagt
     4201 cgctttgata attatctcca attcctcagc gaaatccaaa atcagcgtga aacctatact
     4261 aaagtcagta aaaaattcaa atagaaagtt ggttctctat gtcacatttt aagattgcac
     4321 cgtctatttt ggcggcagat tatgcaaatt ttgaaaaaga gctcaagcgt ctagaaaaga
     4381 caggtgtgga gtatgtgcac attgatgtca tggatggtca tttcgtgcca aatatcagct
     4441 ttggggcgga tgtggttgcg gctatgcgtc ctcatagcaa gctggttttt gattgccatc
     4501 tcatggtgtc caatcctgaa aatcatattg agacctttgc acgtgcaggc gcagatattt
     4561 taaccattca tgcagaagca acggttcact tgcatggaac cttgcagaaa atccgtgctg
     4621 ctggtatgaa ggtgggtgtg gtcatcaatc caggtacgcc gcttgtgacc attgagcctg
     4681 tgcttaactt ggtggatcag gtgcttctca tgacggtcaa cccaggtttt ggtggacaag
     4741 cctacattcc agaggttgcg gataagattg cagatttggt caaactccgt gaagccaagg
     4801 gcttggcctt tgatattgaa gtggacggcg ggattgacga taagactatt cattcagcca
     4861 aaagtgcggg tgccaatgtc tttgtggctg gctcctatct cttcaaagga gacttggatg
     4921 ccaacgttgc caaattgaga gctgccctca atgattaaga ttgctgttat tgcaggcggt
     4981 tcctttgact gccttcctga gcctgccgac ctctatgtgg gggtagatgc aggctctctt
     5041 cgtcttctgg accattccct gcctcttgac tgggctatcg gtgattttga ctcggtgacc
     5101 tctgaggagc tggggcagat aagggagatg gcagagcagt ttctgcaagc tcctgctgaa
     5161 aaagatgata cagacttgga gttggcttta aaggaaatct tcaaggccta tccgcaggcc
     5221 caggttcgca tatacggagc cttgggtggt cgcatggacc acatgatgag caatctcttt
     5281 ctggttgcag aacccgactt ggcagcctat atggagcaga ttgaattggt ggacagtcag
     5341 aacgtcgtcc gttttcgacc tgcaggcagt cataggattt cgccgattgc tggtatgaag
     5401 tacatttcct ttatgccgtc ggaccaaagc cacctgacca ttcgttatgc caagtacccg
     5461 ctggatgcca gcaattattt tttcaaaaaa tgctatgctt ctaacgaatt tatagatagg
     5521 gatatagaca ttcaactgga tcagggctat gtggtcctga tctacagtaa ggacaaggat
     5581 tagatggata ttgtactact tattttgctg atactggtct tgcttgcctt ggtttttctt
     5641 tatggaaaat ggcagagctt ggccctgctt ttgcaagatc aagccgaaga tacagcagac
     5701 aacttgtctg accagctcag ctatcaacta gaaaatgcaa ctctcaaaca gcagcagacc
     5761 attcaccagg aggtggaaag actccgcacg gagctttacc aacagctaac cgacatccgt
     5821 caagagctga ataagagcca cttggaaacg cgagatgcca ccgaccttcg tttgcaggcc
     5881 attcaggagt ccaatgaaaa acgcctggaa gaaatgcggc agacggttga ggaaaagctg
     5941 gagaaaaccc tgcaaacccg cctacaagcc tcctttgaaa tggtgtctaa gcagttggaa
     6001 tcagtcaatc gtggtctggg tgaaatgcag acggtggcgc gtgatgtggg tagtcttaac
     6061 aaggtgctgt ctggcaccaa aacccgtggc atcatgggtg aattgcagct gggacagatt
     6121 atcgaggata ttttgacacc tagccaatat gagcgagagt ttgccacagt ttcaggctct
     6181 agtgagcgtg tggagtatgc ggtcaagcta ccaggacgga ctgagggaga ctatatctac
     6241 ttgccaatcg attccaagtt tccgctggcg gactattatc gcttggaaga tgcctacgaa
     6301 agtggggata aggaccagat tgacctccat cgcaaaaatc tcctggcagc tatcaaacgt
     6361 tttgccaagg acattcagag caagtacctc aatccgcctg aaaccaccaa ctttggcgtc
     6421 ttgttcttgc caaccgaagg gctgtattca gaagtggtcc gcaatcccat tttctttgat
     6481 gagcttcgcc gtcaggaaaa tatcgtagta gcaggaccca ctaccctttc tgccctgctc
     6541 aattccctct ccgtcggctt taagaccctt aacattcaac ggtcggccga tgatatttcc
     6601 aaggtcttgg gcaatgtcaa gctggaattt ggcaagtttt ctgacctctt ggtcaaggcc
     6661 cagaaacagc tcaatcaagc tagcagcaat attgacaaac tcctaaccac tcgcaccaac
     6721 gccatcgagc gtagtcttcg taccattgac ctgtacgaag atgaccaaac caaggggctg
     6781 cttggcctgt ccccattaga tgaggaagat aatgaaaatt aaccaaatga aaaaagatga
     6841 attcttcgaa ggattctatc taatcaagtc agcagaagtc cgacaaacac gggctggaaa
     6901 agactaccta gctctgacct tccaggatga tactggtgag attgaaggca agatctggga
     6961 tgcccaacca gggaaaatca aggattttac tgctggaact gttgttcaca tgcaggggtg
     7021 ccgtgaggtt tacaacaaca ccccccaggt caatcaactg gttcttcgtt tgccaaaggc
     7081 aggcgagccc aacgaccctg ctgatttcaa ggaaaaacca cctgtggatg tcaaggacac
     7141 caaggactat ttgagccaga tgattttccg catcgaaaat gccacttggc agcggatcgt
     7201 ccgtgctctc tacagcaagt acgacaagga attctattcc tatccagcag ccaagaccaa
     7261 tcaccatgcc ttctactcag gcttgtcctt ccatacagcg accatggtac gcttggcaga
     7321 taaaattggt gacatctatc cccagctcaa caagagcctg ctctttgcag gaatcatgct
     7381 tcatgaccta gccaaggtga tcgaactgac tggtccagac aatactggct acactgtccg
     7441 tggcaatctg attggtcaca tttccttgat tgatgaagag attaccaagg tcttgatgga
     7501 attgggcatc gatgacagtc tggaggaagt gactgttctg cgccacgtta tcctcagtca
     7561 ccatgggctc ttggagtatg gaagccctgt ccgtccgcag attatggagg cggaaatcct
     7621 ccacatgatt gacaacattg atgcggaaat gatgatgatg ctatcggcct tggacaaggt
     7681 cggcccaggc gagatgacca accgcatctt tgccatggat aatcgcgcct tctataagcc
     7741 caatatcgac tgatggcagt taggatagtg tctcgacagc ccctcaccaa aggctggtcc
     7801 acggaccaaa agtacaaggt ccagctagaa gatggtcgcc ttggtctttt gagaatagca
     7861 gagcggccag cctatgaggc taagcaatta gagtttcagt tagttgaaaa cctgtttggt
     7921 ctaggtttgc ctgtggcaga gccactaagt ttttgggcgg atgacttgtc agtctacacc
     7981 ctttatgaat ggatggaagg tcaggatatg aatgacttgg ctaccagtct gtcagaacaa
     8041 accttatatg aattgggctg tcagtcaggg aagtttttgc gaaccttgca tgccctaccg
     8101 attgatcaaa gtctgcgcga ctggaacagt ttttatcagg ctaagattga caataaatta
     8161 gctgcctacc aagcagctag tcattcctat ccaaatggtc aagccatgat agcctttgtt
     8221 caggccaacc gtcacttgct tgaagggaga ccgattgcct accatcatgg agattttcac
     8281 acaggtaact ttctacgggg ccaggatgga agactgaaaa ttttagactt tgaccgctac
     8341 gacataggag acccctggga agaatttaac cgtttgattt tcacggcaga tttgtctcca
     8401 gcctttgcgc gtggtcaggt agatgtctat tttgacggcg ctattccaga ggaattttgg
     8461 aaactcatgg ctctttatgt aacagtaaat agtctaggcg ctctttcttg ggcagaacaa
     8521 gtagattccg aacaaattcc attgatgaag ttgcaagcgc agaagatttc tgaatggtat
     8581 gaatatttta atcatcatct tccaaagtgg tatatgtaag cgataaacaa aatacgaaaa
     8641 agacggactt ttcaatcaaa aaagttcgct ttttgtagtt tgttgtgata taatagaaaa
     8701 aagtgaaaga ggatgatttt atgaaattaa gaagaagtga gcgtatggtt gtcatttcca
     8761 attacctcat caaccaccct tatgaattaa ctagtttgaa tacctttgcg gaaaagtatg
     8821 aatctgccaa gtcatctatc tcagaggata ttgccatcat taaaaaggct tttgaggaaa
     8881 gctctatcgg gcatattgaa accattactg gtgccagtgg aggagttgtt tttactccat
     8941 cgatctctaa ggcagagtct gtcgaaattg cacaggccct tcgtgatcag atggccgaaa
     9001 gtaatcgtat cttgccaggc ggttatatct attcatctga cttgctgtct acaccacata
     9061 ttttgaaaaa tgtcggtcgt atcattgcag atgctttcaa agatgagaag attgatgcgg
     9121 tcatgacagt tgcgactaag ggggtccctt tggccaatgc tgttgccaat gtccttaatg
     9181 taccgtttgt gattgtgcgt cgtgatttga aaatcacaga aggttcaaca gtttctgtca
     9241 actatgtgtc aggatctagc gaccgtatcg aaaagatgtt cctgtctaag cgtagtttga
     9301 aggcaggaag ccgtgtctta atcgtcgatg acttcttgaa aaatggtggc accatcaatg
     9361 gtatgattag cctcttgtct gagtttgact caaccttggt aggcgtggct gtctttgcag
     9421 aaaaccaaaa aggggaccgt agcgtagcta actacaagtc tctgctcgct gtgaccgaca
     9481 tcaatgtcaa ggaaaatcgc gtggatgttg agttgggaaa tatttttgaa taacatacat
     9541 gcaaaaaaga gctggttttt cagctctttt ttactattct ttcaaaactc tccgaccgcg
     9601 cctccacgct gatttcttct aaagtcagcg ggtgggtcag gctgagtttt tgggcgtgga
     9661 gcatgaggcg agggggagct actcgactgt aaagcgggtc gccgattaga gcgtgaccgt
     9721 ggtgggctaa atgaactcgg atttggtgtg tacgacctgt ttggagacga caggaaacta
     9781 gactattttt accaagggtc ttcaagaggc ggacctgggt aagagctgtc tgccccttgc
     9841 ggttgtcgac cactcgcttt ctacggtcat gccggtcccg tccaatctta tcggtaatag
     9901 tccagtcagt catttttagc tggccttggc agagggctag gtagtcacgg tagatgacct
     9961 tgtcttccaa gagccgattg agaatgggca ggataaaggg atttttggcg aagagaatgg
    10021 ctccgctggt ttccatatcg agtctgtgga ccacatagca agtctgtccc acataggcag
    10081 acacatggtt gagcagggca atttctgtcg gatcattgcc gtgggttttc atgccttcgg
    10141 gcttgttgac gataatgagg tgctcatctt gatagagttc ctccaccaag tccgcctgtc
    10201 ccagaggaat gcttttttca ggataatctt cctggtcaaa ggtcaattct aacaagtcgc
    10261 ctgcggtaat cgggcactgc cagttaatca gctcaccgtt gacacggacc tgtttcttgg
    10321 tgcggagaaa gtggcgaatt tttctgggaa tgagaaaata atcttccaag acttccttga
    10381 cagtcagttg aggaaaggct tggggaatgc gaatatcaat tttcatctta taggctggtt
    10441 gagtgcttgg gttgcacttt ttcgtttgga ttgaggtagt tcatggcatt gttaacagcg
    10501 gttggggctt cgcccagtcc agtcgcgatc aggtcaatct tgccctcgta gaagcaacag
    10561 tcaccgatgg catagatacc agggacagag gtttcttgct tgctgttgac gacaatgcgg
    10621 tggcgattga gttccaatcc ccattctttc agcgttccga cagatgattt gaaaccgtag
    10681 ttgacaaaga ggtggtcaaa agacagggtc agcttgtcct cgctcttcac cttatcgaaa
    10741 tcaatggcag aagctctgcc attttcccca gaaagccctt tagggataaa aggtgtgtga
    10801 atggtcacgc tggactgctt gagctcttcc acgctgtgct caagggctcg gaagttatcg
    10861 cggcggtgga tgatctgggt ggtcttggcg atcttttcaa aggccaggga ccagtcgacc
    10921 gcagagtcac caccacccaa gacgacgatg tccttgtcgg catactgctg gatattagac
    10981 acgtggtaat ggacgttgtc aaagctgtca gcaccatcaa tctccagcgg acgaggcttg
    11041 aaggccccgc cacccatagc gataatcagg gtcttggtct ggtggttgcc cttgttggtt
    11101 gttagggtaa tggtttcccc aggttcgata gcagtcaggg tttcattgag gcagatggtg
    11161 gtgtcaaatg gagccagctg ggcaaggagg ttgtcggtca actcctgtcc cgtcaggctt
    11221 ggaaaggctg gaatgtctaa aatggccttc tcaggataga gaatggcagg ctggccaccc
    11281 agttggggaa gggagtcaat gattttgacc ttggcttgac ggagatgggc gtagaaggcg
    11341 gcgaagagac cgactggtcc gccaccgatg atagtaatgt catatacttg tgtcatagga
    11401 atcctcttct ttgttcagaa tgaccctatt ttatcatatt tttggaaaaa attccctgta
    11461 tgttgtcaca agttatcgca ggcttttttc tgctagtttt taggaaatgg tgtataatgg
    11521 tacaaaattc actagaaaga ggtaaagtat ggacgatttg caacatcagc cgattgaagt
    11581 tccagaagat ttccaaattg atcatgaaga ccgacctagc cgtcgccaca ggggaaatcg
    11641 tggctctatt gataaaccaa agaaaaaatc acgtattccg gagccgattc gtaaattttg
    11701 gcgccgttat caactgacta aaattttaat cattttaatg gtcctgacag ttcttacagt
    11761 aggtggctat ctatttttcc ttgcaaagac agctaatgtt ggtgatttac agcaggcgct
    11821 aaaagccaca acgattattt acgataaaga tggtgcggaa gctgggacat tgtctggtca
    11881 gaaggggacc tatgtagagc tggatgctat ctctgataat ttagagaatg ccgttatcgc
    11941 tacagaagat cggagcttct acaaaaattc aggcattaat taccagcgta caattttggc
    12001 tgccttgacg ttaggtcggt ctggaggtgg ttcaactatt acccagcaat tggcaaaaaa
    12061 tgcctttcta actcaggatc agaccatcag tcgaaaggct cgagaattct ttttggctct
    12121 ggaaatcaat aaaaagtaca gcaagcaaga aattttgact atgtatctca acaatgccta
    12181 ctttggaaac ggggtctggg gagtggaaga tgcaagccaa aagtacttcg gtattccagc
    12241 tagtaatctt actcttgagc aagcggccgt catagcaggt atgctcaagg gacctgagat
    12301 ttataatcct tattattctc tagaaaatgc agttaatcgt cgggatacag tcctgcaaaa
    12361 tatggtcaat gctggttata ttgatcaggc gactgcagat gcaggtttcc aagtagacct
    12421 agccagtcag ctttcagata cctatacagg gaaacaaagc aattattcct atccatccta
    12481 ttttgatgct gtgattgctg aggcgattga gcgatacgga ctgaaagaat cagacattat
    12541 taataacggc tataggattt acacagagat ggatcaaaat tctcaagcca gcatgcaggt
    12601 gatttttaat gatgagacta tgttcccaac atcaagtttt gatggtaccc atgctcaggc
    12661 tgccagcgta gctcttgatc ctaggactgg tggagttcgc gccttggttg gtcgagttaa
    12721 cagttcagag gatgcggtct ttcggacctt caactttgcg acacaagcca agcgcagtcc
    12781 tggttcaaca atcaagcctc ttgttgccta tgctccagca gtagcggcgg gctggtcgat
    12841 tgatatggct ctggataacc atacagaaac ctacggcgat tacaccttgc ataactatga
    12901 ttattcgact tctgatacca ttccaatgta tcaagctttg gctctctctt acaatttacc
    12961 agtagcttat atagtgaaca ccttgggtat tgacaaagcc ttcgagtatg gacaaaagtt
    13021 tggacttgat atggaaaatg tcgagaaagt tttgggtgtt tcaattggtt ctggagtgga
    13081 gaccaatcct ctgcaaatgg cccaggccta cgctactttt gcgaataagg gtgtgatgaa
    13141 ggatgcccac ctcatcactc gtattgaaac ggctagtgga aaggttttag cagagcatcg
    13201 agaaacaagt accaaggtga ttgaccgtgc tgtcgcagat aagatgacag caatgatgct
    13261 tggtacctat accaatggta cgggagtgac agcagatacg gccaattatc atatcgctgg
    13321 gaaaacagga acaaccgaaa cgagttttga tgtcaatctc gtcaatgatc agtggcatat
    13381 tgcctacaca cctgatttgg tcatcagtca gtgggttggt tttgagcaga cagatgaaaa
    13441 tcattatata gatagcagta gctactggaa ggctccgtcg gtcttccaga ctgtggccaa
    13501 ttcaatcttg ccatatactg caggaagtaa gttcgaagta gaaaacgcct atgcacaaaa
    13561 tggtattgta gaagtagctc cagatgaagc tgcgcaaact gaatcaagcc ttggtcaaga
    13621 acaggtacaa gaaatttcag aacaggcgaa aaacttgatt gagcaaacca aacaaaatct
    13681 aatcgatgcc cagttaccag aacgagcaaa aaccttgtgg gataacataa cgtcttggtt
    13741 tggagagttg tcaaatccat aaaaacatgg tagaatgaaa aggatggagg gctttatggc
    13801 actaaaaaaa gcaagcctag cctgtactgt ttgtggttct cgaaactatt caatcagcct
    13861 ttcgagcaat cccaagccaa cacggttaga agtaaataaa ttttgtaaac actgtggaca
    13921 atacactgtt cacaaggaaa ccagatagga gtcacgcatg aaatttatca aagacattat
    13981 ccgcatttta aaagatacgt cttggccaac tcgtaagcag agctggacgg atttcgtgtc
    14041 tgtagtggaa tatactgcct ttttcgtagc ggtggtttac ctctttgact tgattttgtc
    14101 acgaggaatc atgagcttga tcaatctttt ctagaaaata cttgccattt gtaaaatgga
    14161 agtgatataa taaggaaaag cgaaagcctt cgggcttttt ttagcgtcta aaaattccgt
    14221 gtttccaaag aaaggaagta acaatgtacg atagttttga taaaggctgg tttgttttgc
    14281 agacctactc aggatatgaa aataaagtga aggaaaactt gctccaacgt gcccatactt
    14341 ataacatgtt ggaaaacatc ctccgtgtag aaattccaac tcagactgtt caggttgaaa
    14401 agaacggtga ggtcaaggaa gtggaagaaa accgtttccc tggttatgtt ttggttgaga
    14461 tggttatgac cgatgaagct tggtttgtgg ttcgtaatac gccaaatgtt acaggtttcg
    14521 tgggttcaca cggtaaccgt tcaaaaccaa ctccactttt ggaagaagaa attcgtcaaa
    14581 tcttggtgtc tatgggacaa accgttcaag aatttgatat tgatgtcaaa gttggcgaca
    14641 ctgttcgtat catcgacggt gccttcacag actacacagg taaaattaca gaaatcgaca
    14701 acaacaaggt taaaatggtt atctctatgt ttggtaatga taccattgct gaagttaatt
    14761 taagccagat tgctgagttg taataagttg ttaaagcgca gggaagcctg tgctttttct
    14821 tgtttttgag ggctaattca aaaaaaactt gccaaagatt taaaaatttt atagaattag
    14881 gtgtacctaa cttataaatt aattaaactt aaaaaacgga ggttttatga aacagaagtg
    14941 gtctcagatt gagaataaac aacgctttag tatcaagaag ttaagtgtcg gtgtggcatc
    15001 ggtatcaata ggatttttta tcacgggtgt tccgatggtt caagcagata ctagtggaga
    15061 aggattggag tctacagttg cggtcgcaac agatatggat agtaggcaaa attctgcggt
    15121 agagaaaaaa gaagatggac cattatcaga tgatccagtt aaaacggagc aggtggatga
    15181 accagttgct gaagagggag tggtcgaaga agttgtagat acagaggcgg gtgaagaatc
    15241 aggtcttctc acagatcaag ctgcgaccga gatagaaact acagctggta agacaacaga
    15301 tgagtcaaag gaaaaagaag acattagcgg aaaagaagct agtgctcctc aaacaatccc
    15361 gcaggaatca cagcttgagc cagaagaggt gacaacaggg cgctatattt tacaattttc
    15421 cgaagagaac cgaaatcttg tattggataa attaaagaaa attgatggcg ttaaaattgt
    15481 tcatgagtat aaggaggttt taacgggagc atcagtagag gtagggaaag aaagtttgtc
    15541 tgatgtgaag gcaattaccg aattaacctc tttagaagag agtcgccgta tccgaccgac
    15601 tcttcatact gctaaacagc tggttggtgc cttaaaagca agttctaaat atcagacaga
    15661 tggtcgcgga atggtgattg cagtcattga ctccggtttg gatattaagc acaaagacat
    15721 gaggttggat gatggtgtca ttcctaaaat taaggacatt actccatcta cgacagggac
    15781 atacacattg aaagttcccc atggctataa ttatgtatct ggcaacgata atctctacga
    15841 tgatacccac gaaccacatg gtatgcacat agcaggaact ctggctggaa atgcaacaga
    15901 cgaagaagtt gcgtctaaaa agggagttga tgggattgct cccaacgcac aactgctagt
    15961 ctacaagatt ttttcaaatg accctaaaaa ttataaagct gagacggagg atgctgctta
    16021 tgctgctatt gaagatgcta tcaaacacgg ggcagatgtc atcagcttga gtgttggtta
    16081 ttatgatagc gggctaccag ggaatgctta ctacacgatt gctaagaggg cagcagaaaa
    16141 gggaattatc attacagcgg ctattggtaa tgctggtgcc tcctcttcag acacctcctt
    16201 tgacctgcat acaaataatg ccttaggagc tgtggatacg gcaacaacag ttggtgtagc
    16261 agcgactccc gcagttatcg cggttggttc ggcgagaaat acccatcttg ttcaacgaga
    16321 atttatgctg aatggacagt cttttggtta ctatcctatt ggctatacaa cgcttacaga
    16381 gggaaaatat gaatttgtag atgctggaaa tggtcattgg gaagaagtgc aagggcttga
    16441 tttggctggt aaagtagcgg ttatcaaaaa agataagttt gacttgaaag atgctgttcg
    16501 taatttgaaa ttcaaggatg ttgctggaat tattgtgata aatactgatc agggatggaa
    16561 caaggattat tataggaccc atcagttgtt ggtagatgat aagactttgc tttcctactc
    16621 ctctatctgg ggaattagtc ttagcgggga agatggaagg cgattattag aagttgccaa
    16681 ccaatcgcaa ggcaacactg gtttggttct taaacctaca attggaatga agaagttgat
    16741 tgaagtgccg actgtatcag gcttttctag ttggggacct acggtcaact tagaattgaa
    16801 gccagagatt gtggcgccgg gagaggatgt ttatgcaacc ttgaatgaca atcgttatgg
    16861 ctccatgtca gggacttcga tggcttcgcc aattgtggcg ggtgccagcg cacttttatt
    16921 gccacggatt cgccaaatga caccaccaga aggcatgact aggatggatt tgctgagaat
    16981 cattttgatg aatactgcta cccctttggt tgatgttcta gattcatctg ggcatgcttt
    17041 ggaaaattct ccgagacaac aaggtgctgg tctgttgcag attgatagag cctttgaaac
    17101 agatgtgatt cttcaccacc gtctaaaagg aggggtggaa ttaaaagaaa tagggcgtga
    17161 gacagaattt gaggtaacct tggaaaatct gggaaaccaa caaagaagct ttgctatttc
    17221 agctgggaaa gtgttgacta gtcaagatgt tcctgttgat agaataggac gttctggaaa
    17281 agtagttaaa gagattcatg cgacagaaat caaggggtcg agtattcatc tttctgagca
    17341 atccattcaa ctaggtccaa aagagaagag aaccattcgc ctgaaattgg atgcaggaga
    17401 agcgaaagac cagtttgcag aagggtatat ttacttcaaa tcattgacgg aggggcagtc
    17461 tgacatcagc ataccttatt ttggctttgt tggtgactgg tcaaaagaaa ggattgttga
    17521 tgcaccggct tgggaaacca gttctaaatt gaaactgacc tcagttttat ctagctataa
    17581 acacaataag tctgggcgct atattgaact tgggcgtgaa aagattcaag ataatcaatc
    17641 gcctctcaat ccagacaata tcgcaattca aaaccagcat tcggacagtc agattgggaa
    17701 tgcctttgtt cgatttgcgc tattgaggga tattaccaac tatgatttgg atattgtaaa
    17761 agaagctaca gaagatgctc ctgttttaag gcgaattgat acaggaacca tgctgtctcg
    17821 tgttcgctat gtagattatt ttgaaagtct atcggagtat tccaagcttc gtactccgat
    17881 agaattgcac cgttgggatg ggaaagtgta cgatgcaagc aatgatgaaa atataccagc
    17941 accggaagga caatatttct ttagattgcg ggtaaaaaat aaagaaaatg gggcctatca
    18001 gtatacctat ctgccagtca aaattgataa tcaaaagcca gagattgtcg cgattgatac
    18061 caatcgtcta tccagccata gagaactggt cgtcacagcc aaggataata ataaagtttg
    18121 ggaggttcga gctaatctaa atggagaaga tcttcttgtt gaaaaggttg tagatgatgc
    18181 aggtcaacta cattaccatc tcaaagaagt ggaactgcca ctcgatgcta aaaatcatct
    18241 ccgtgttgag gtgatggata tagcgggtaa tgtggttgct gttgaaaaag atttgatggc
    18301 gcctgtaatc cagtttaaaa atttggaaga tttgatggca attcgcagta agaaaactgt
    18361 agaaattaaa gcgaacgtct cagcgcaagt ttccgatgta caggctaact tggatgctca
    18421 ggctgtgaat tactctcttg agaatggtca actatctctc cagattccag agcaatcaga
    18481 tggacgtcat agttttgaat taatcttgaa agataaggat ggcaacctta tttacactaa
    18541 aaccttgaat tatttggtag ataatgaaaa gccgacgatt gatcttgata ttgaaaaaga
    18601 tgaagaggat gaagaggtta tccagattgg taaaaatggg cgttttacat tgaaagggaa
    18661 agtgagtgat aatgttagtc ttcctaagga catcaaactt tactattcca atctagacat
    18721 tggtaaggga gagcggaaaa tcatagatgt aaaagaagac ggcagttttg agcaagactt
    18781 ctttaaatct gattttccta gagccatcat gttaactgcg gtagatgaga agggaaataa
    18841 attaaaggac cttcgtatca atacaagtcc agagagtctg gatgaggagg aggaaacaga
    18901 agttccgatt actgtcaata actggctgat tgacccaatt cgtttcaata aagaaagtct
    18961 tggtcgtgag ctagatagcg ggttggtcga cttcaaaaag caagaagatg ggacttatct
    19021 atttacattt gaaatagaag cagaaaccga acaagctcat tctgtgcgca tcaatggtgg
    19081 tgaaaaacgc tattttgagg atgggaagtt gacctatcca gttactctga ttgaagaggg
    19141 aaatgttgtg gatataagtg tgtacaatga ggcagatgag ttgacttaca cgaagaaata
    19201 tcagatgttg gttgatacag aaaaccctgt tttacagctg gaaaatgagg tgctaccatt
    19261 ggaaagacag gtagtggata gtgaagaaga tgaagacgag gaaaatcagt atgcaggagt
    19321 cctcctggca gatgctgatg ggcacttgac acttaccgga agcgccaagg ataatggaat
    19381 ttattggtcc ttgaagatca acgaagattt tgtagctcga ggaggcttct ggaggcagta
    19441 tggaaataat gaaaaagcct ttcgctatga gctacatagt ctgaaagacg gggatacagt
    19501 caaacttgat ttgagtgaca gttttggaaa tgctgttgtg aagaaatata aggtacggtt
    19561 aaatgacaag gaagtatctg agcaggttcc tgaaaaagac cttcatgtgg agcgatcaga
    19621 taaggatcaa acccctagca ttcctattct gaaatcggaa gcgcatatac caatgccaaa
    19681 agaagagaat tcacttgctc cgcaaacagg atccactgaa atcgcgctgt taactggcga
    19741 tacaagagaa gatggtgtgg agcatttggg gaaacttaca aaacatgagg agccactagg
    19801 aatttctgac gagagaatag aagtgtccgt acctcacaga gaattctttg aaagaagcgg
    19861 aataggagag acaggtgcat tagcggcgga tactagtggg aaactgcctc aaactgggga
    19921 tagcctggga agtgtcttta taagtacttt gttaggtcta tttggtggag ctatggccct
    19981 cggaaatttg aagcgaaaag aatagtaaaa aacctaggaa gattcctagg ttttttagaa
    20041 tattaaaagg cggtagacgg atttgaaccg acgatcaagc ttttgcagag ccgtgcctta
    20101 ccacttggct ataccgccat aacaaagaat attctaccga aaaaaaagct gaaagtcaag
    20161 aaaaatctag tgactgtttg tgactgataa caaatctata aaacagttgc gaaaacatag
    20221 gagaatacga cgaaaaagat aggagtactt tttgagtatt gatggctagt accactctct
    20281 ttgtttgata gaaaaagagt ggagcaaaaa gaattaagaa acactttggg taagtgaatt
    20341 catttttact gcaaggaatg ttaagggaac agaattacga taatagtgca cctctttaag
    20401 aaagatttgg aggtgataga gtttattttt gctaaataaa gattaaacca agaagaatat
    20461 tccatctaaa acggttgttt ttatttacag tatattgcaa agataactca ggtacttgat
    20521 tagattaact gaaaaaatgt gggaacatga tataataaat tgagtaagag attaaaaaat
    20581 agataggatg acaagcagtg tttcttacaa attttcatat aaaacataga tgctttgtcg
    20641 cttagatagg agaaaatcat gattgtttta ataacaggag catctcacac tgggaagact
    20701 gtgctagctc aaaaattact tgaaaaatat cactatcctt atttatcaat agaccatttg
    20761 aaaatggggt taattcgtag cggcaatact aaactttcag tcgaagaaga tgataaactt
    20821 acagcatatc tatggcctat tgttcgtgaa atgattaaaa ctgctataga aaatgagcaa
    20881 aaccttattg ttgaagggat ctatatccct tttgattggg caaacgactt tgcaacagcc
    20941 taccttgacc atatcagata ttactgtctt gtgatgagtg aaaactatat tcagaaacac
    21001 tttggaagca taaagaaaca tgcacgcctt atagaaaatc gtatagatga tgaatggtgt
    21061 acgatggaaa gtgttttaga agaaaatact cgatttttta gaagggcgaa aaaatatgat
    21121 gcaaacatcc tatggattga tgagagttac gagatagaga ttgatttatg atagagagac
    21181 attgtatcac agtttatcta agggatacaa ctaagaggaa gtaacaccag ataggcgtta
    21241 cttcctttct atttctaaga aatagaaagt aaaatggatg aagttaaaaa taataaaaaa
    21301 gtagtaacat aaagagagac atatcccgat ctatgagctt ggcataatct agagatttgt
    21361 gtactgaaat gcttgatttt cacctaaaac tttggtatac ttactaagtt gtctaaacaa
    21421 caaatactca tcccggacca attgtggtct tgttgccgaa aggttgggct gcaagtcgaa
    21481 gttcgggaga ggagaaaaaa caaaaggaga aatactcatg gcagtaattt caatgaaaca
    21541 acttcttgag gctggtgtac acttcggtca ccaaactcgt cgctggaatc ctaagatggc
    21601 taagtacatc ttcacagagc gtaacggtat ccacgttatc gacttgcaac aaactgtaaa
    21661 attggctgac caagcttacg aattcatccg tgacgctgca gcaaacgacg cagttatctt
    21721 gttcgtaggt actaaaaaac aagctgctga agctgttaaa gacgaagcta tccgcgctgg
    21781 tcaatacttc atcaaccacc gttggttggg tggaactctt acaaactggg gtacaatcca
    21841 aaaacgtatc gctcgtttga aagaaatcaa ccgtatggaa gaagatggaa ctttcgaagt
    21901 gcttcctaag aaagaagtag cattgttgaa caaacaacgt gctcgtcttg aaaaattctt
    21961 gggcggtatc gctgacatgc cacgcattcc agatgtaatg ttcgtcgttg atccacacaa
    22021 agagcaaatc gctgttaaag aagctaagaa attgggtatc ccagttgtag cgatggttga
    22081 cacaaacaca gatccagatg atatcgatgt tatcatccca gctaacgatg acgctatccg
    22141 cgctgttaaa ttgattacag ctaaaatggc tgacgctatc atcgaaggca accaaggtga
    22201 agacagtgta gcagcagttg aagctgaatt ggcagctgag ccagcatcta cagaatcaat
    22261 cgaagaattg gttgaagttg tagaaggaaa ataagtaacg aaggcgttaa gaaaagcgtt
    22321 agatatatct aaattctagc tttttagtcc gagtacattc tacattattt atcagacaca
    22381 aaaacaatcc tagaggggca gggctgagcc cgctcctcta ttttataaat acaaacaagg
    22441 agaatagaca tggcagaaat tacagcagct ctcgttaaag aattgcgtga aaaatcaggt
    22501 gctggcgtta tggacgcgaa aaaagcattg gttgaaactg acggtgatat cgaaaaagcg
    22561 atcgaattgc ttcgcgaaaa aggtatggct aaggcagcta agaaagctga ccgtgtagca
    22621 gctgaaggtt tgacaggtgt ttacgttgat ggtaacgtag cagcagttgt tgaagtgaac
    22681 gctgaaacag acttcgttgc gaaaaatgct caattcgttg aattggtaaa cactactgct
    22741 aaagtaattg cagaaggtaa accagctgac aacgaagcag ctcttaaatt ggctatgcct
    22801 tcaggtgaaa cccttgaaga agcatacgta aacgcaactg caacaatcgg tgagaaaatc
    22861 tcattccgtc gctttgcttt ggttgaaaaa acagatgctc aagcatttgg tgcttaccaa
    22921 cataacggcg gccgtatcgg tgttatctca gttgttgatg gtggtgacga aactcttgca
    22981 aaacaaatct caatgcacat cgctgcgatg aaaccaactg ttctttctta cactgaattg
    23041 gatgagcaat tcgttaaaga tgagttggca caaatcaacc acaagatcga acaagacaac
    23101 gaaagccgtg caatggttaa caaaccagta ttgccattgt tgaaatacgg ttcaaaagct
    23161 caattgactg atgaagtgat tgcagcagct gaagaagcta tcaaagcaga attagcagca
    23221 gaaggcaaac cagaaaaaat ctgggataaa atcatcccag gtaaaatgga tcgcttcttg
    23281 cttgacaaca ctcaagttga ccaagcttac acacttcttg cacaagtgta catcatggac
    23341 gacagcaaaa cagttgaagc ttacttgaac tcagtgaacg cttcagttgt agaatttgct
    23401 cgtttcgaag tgggtgaagg tatcgagaaa gcttcaaacg actttgaagc agaagttgca
    23461 gcgacaatgg cagcagctct tggtaaataa ttgctagtct tcattttatc tttcattact
    23521 tcgttaactc gccttgccgt acttcagtac agcctgcggc tcgtttccta gtactaaaag
    23581 caaactgata gactattcaa aatcacaaga acttccgaaa gggagttctt ttttggcaaa
    23641 atcggtctta gggcggatgt cggatatgct tgattcggtt atactattat caatcctaaa
    23701 tatttttcat aatctcctta aaaagcaccg gccttgtggt cggtgctttt tgttagattt
    23761 ctaaaatgta agcatcccag ctaggcttgt ttggttgtcg gaattggtca gctaaatgca
    23821 tggcgatagg atcgcagtca tcagcctcaa agctaatttg ctcatactgt tcaaatagtt
    23881 ggcaaataac tgcttggatg aaggaaccaa agctttgttc atcttttccg catacgtagg
    23941 caatttcatc ggtatctatg aaggcacaat tttctgagtc tgtataaacc agatcaggta
    24001 agcctttttc aaattcttct tgactggcag tccaaggatt gatgtcttgg tggactgttt
    24061 gatagtggtc aaaaatttgc tgacagacta tttgataagg tgcggtgcct ttgaggacta
    24121 tgcttaagtt ggaagtgcct gtttgactga gatagtcttg cttggtcact tcgaactcat
    24181 aacatttccg cttgcagaca aagccagctg tggtaaggaa atggattctt tcctgctctg
    24241 cagaggagag cataacttga agaggttttc cgcctaagga agtctgtagg gactggaaga
    24301 gctgctttgc tattttgcaa tcgaaatcaa ccaaatctag tcgcagatag atattggtct
    24361 ggtggtaggg attttggttg ctgtgaatct tgccgagtgg tcttccttga taagagatgt
    24421 ggaaggtgtt ggcttggtag gaaatgtcta gcattagtct tgaataaaag ccgcaatctc
    24481 atcttccgtc atgctggaat cgcagcggac ttggacacca gtagatttag cgaccaggag
    24541 gcctggaaca gttgggactt ggtagtggtc gcggagggcc tggagttcag ggctgacttg
    24601 gctggagtca aggaagtgga tggtcacttg cttgtcttgg gcaaccttgt ggagttttgg
    24661 tgcgaagcgg ttgcagtaag ggcaggttgc acggccgatg aagaggaccg ctccctcatt
    24721 ggcagttaga aggctctgtg cctgctccac gtttacgctg gtaaaatctt ggatgtagtc
    24781 ttgaaagttc atggggttct cctttttttt cctttattat agaagaaagc agagcaaatt
    24841 tcaaaaattg gctacgtttg tgtggaagtg aggctttcgc tttcaaaaag tggataaaaa
    24901 tagtataatg gtcaaagata gtcaaagaaa gaggcgatag tatggcgatg aaaaatacgt
    24961 cggattatat cgaagaacat atcaaagcga ttttagatca ggtcagtgta gccgagttgc
    25021 gtcggagcga gctggccagt cgatttgaag tggtgcccag ccagattaac tatgttatca
    25081 aaactcgttt tacagccagt cgaggttata ttgtcgagag caagcggggg ggtggtggct
    25141 acattcgcat tgggcggatt accttttcgg acaagcatga gctagtccat gatttgctga
    25201 agaatatggg tgatagcctg tcggcaactg tttttgcgga cattctgcag ttgctctttg
    25261 acgagcagtt gatgacggaa cgtgagggca atctcttgtt ggctactagt acagatgagg
    25321 ttttagggaa cgaggctagt gaaattcggg ctcggatgat gtaccaaatt ttacgtcgat
    25381 tggataggaa agaggagtag ttagaatgaa gatttcaaga gggttacagg gtgtctatga
    25441 agatgctcaa ttgattgcac agcgttatag tagtgactat ttggagacct ggcacttgtt
    25501 gttagccttt gtcatcaatc cagataccgt tgcgggagct attttagcag aatatcctgc
    25561 ggatgtattg gactatgaac gtgcagttta tatggtgatg gggcggcgtt accatgaaga
    25621 gttagagagc tttttctttc ttccatcgtc caagcgggtg aaggaattgc aggtctttgc
    25681 cgagaagatt gcggagattg tcaagagtaa ggggctagga acggagcata ttttcatggg
    25741 aatgctcttg gacaagcgtt cgactgcctc acaaattctg gatcaggtcg gttttcactt
    25801 tgaggattcg gatgataagg ttcgttttct ggatttgcgg aaaaatctgg aagccaaggc
    25861 tggctttacc aaggagcatc tgaaggctat ccgcaccatg acgaaaggtg gcaagcccaa
    25921 gcaggcaacg gttggcaata tgatgggcat gacccagtca caaagtggtg gcttggaaga
    25981 ctatacacgt gatttgacgg ctttggcccg ctcaggtcag ttggagccag tcatcggacg
    26041 ggatgaggaa atttcccgta tgcttcagat tttgtcgcgg aaaaccaaga acaatcctgt
    26101 cttggttgga gatgcgggtg ttgggaaaac agctctggca ctgggtctag cccagcggat
    26161 tgctaatgga gaggtgccag ctagtcttgt caatatgcgg atcttggaat tggacttgat
    26221 gaatgtcatt gcgggaacgc gtttccgtgg ggattttgag gagcggatga acaatatcat
    26281 caacgatatt gaagaagatg gtcgagtgat tctcttcatt gatgaactcc ataccattat
    26341 gggatcgggg tcagggattg actcgatcct ggatgctgcc aatattttga agcctgctct
    26401 gtcccgtggg actttgcgga cagttggagc aacgactcag gatgaatacc agaagcatat
    26461 tgaaaaagat gctgccttag tacgtcgatt tgccaaggtg accattgagg aaccgagtgt
    26521 agcagacagc gtagcaattt tgcaggggtt gaagccagcc tatgaggctc accacaaggt
    26581 gaccatttcg gatcaggcgg tggtaacggc ggtagcctat gccaaacgct atctgaccag
    26641 taagaatttg ccagattcgg ctattgattt gctggatgaa gccagtgcga cggttcaaaa
    26701 tcgtgccaag ggacaggtag aagaaggtgg attgaccgct ttagaccaag ccttgatggc
    26761 tgggaaatac aagacggtaa cgcagctctt gcttaaggct caagaggcgg aaaatcaggc
    26821 gactagctat agcttggaag tcacagaaga agacattttg gcaaccctca gtcgcttgtc
    26881 aggtattcct gtcaccaaac tgagtcagac agatgccaag aagtacctta atcttgaaca
    26941 ggaattgcac aagcgtgtta tcgggcagga agaggcgatt tcagctgtca gccgggcaat
    27001 tcgccgcaac cagtcaggca ttcgcactgg tcacagaccg attggttcct ttatgttctt
    27061 ggggccaaca ggtgtcggta agacagaatt ggccaaggcc ttggcggaga tcctctttga
    27121 tgacgaatct gccttgattc gttttgatat gagtgagtat atggagaaat ttgcggctag
    27181 tcgcctcaac ggtgctcctc caggctatgt tggctatgaa gaagggggcg agctgacaga
    27241 aaaagttcgc aacaagccat actctgtcct actttttgat gaggtggaga aagcacatcc
    27301 agatattttc aatgttcttt tgcaggtctt ggatgacggt gtcttgacgg acagaaaagg
    27361 tcgcaaggtt gatttctcta atacggtcat cattatgacg tctaacttag gggcaaccgc
    27421 tttacgtgat gataagacag ttgggtttgg ggctcttgat ttgtctaaga gtcaggaaca
    27481 cgttgaaaaa cggatttttg aggcgttgaa gaaggcctat cgtcctgaat ttattaaccg
    27541 gattgatgaa aaagtggtct tccatagcct gacagaagca gatatgcagg atgtggtcaa
    27601 ggtcatggtc aaaccattga ttgccgtggc ggccagcaag ggtattaccc tcaaattgca
    27661 ggcttctgct cttaaactct tggccaaaga aggctacgat ccagaaatgg gtgcccgccc
    27721 acttcgtcgc ctcctccaaa ccaagttgga agatccattg gcagaaatgc tcttacgtgg
    27781 agaactgcca gctggtgtga ccttaaaagt aggggtcaag gccgagcagt tgaagtttga
    27841 tagtgtgaaa gcaggttaga cctgcttttc tttcggcttg ctatcttttt ataaaaaaat
    27901 ataaaaaact cttgactttg gtttgagaaa agagtaggat ataaaatata aaaaacaata
    27961 aaaaggtaga ttatgaaaca agcagtagca gttatttttg ggacctttgc tcccatgcac
    28021 aagggtcata ttgacctgat tcaacgagcc aagcgggaat gcgatcgggc ggtcgtcatc
    28081 gtgtcgggct ataaaaatga ccgtggtcat cagattggtc tcggtttgca gaaacgtttc
    28141 cgctatatcc gcgaaacctt caacgacgag cctctggtat ccgtctttaa gctagacgag
    28201 gagggaatgc ccccttatcc agaaggttgg gcaccttggt tagaagcctt gcaggccttg
    28261 gtggcgatta aggagaacga agagctcgtc ttctacgttt cggagcagga atatgccgaa
    28321 gagttgcgtt ctcgtggttt caaggcttcc tttacagagc gaaattttgg catttcagcc
    28381 actcttatcc gcgagcagtc ggctaagtat tggaatatga ttgccaagcc tttccgccgt
    28441 catttttcta agaatatctt ggtggttggc tctgcttcaa atggtaaaac cacgctagtg
    28501 cgggatttgg ggcgttatta ttcctgtcct gtttcgctgg aatatgcccg ctactaccag
    28561 caacgttaca atgtgcgtga tgatgagttg acaggcaagg actacaacta tctcttgacg
    28621 ggccagtatc gccagacctc agacctgatt gactcggata ccaatcgtgg tctggtcatt
    28681 gcggacacca acgcgaccgt gacggaagcc tactacgact actacatcgg cgaaacttct
    28741 agttctttcc actcgctctg tgctgatacg gttaagaatg aaaaatggga cctgattatc
    28801 tttgttctac cgacaggctc ttatgtggat gacggttttc gggatatgac aatggctgac
    28861 gcagaaattc gcaatgcctt taccgaacat ttgaaagaac tggttgctaa aaatcacccg
    28921 caggcttcgc tggcctttat cggtgggtct tatgcagaaa attatgccaa ggctatcgag
    28981 ctaatcgatg ctatctatca ggaattttag gaggacatta tggaacaact taccaaattc
    29041 atcgaccgtt ttcaagcttc cttgaagcag gtaccagaaa acatgtctgc tatcgcaaat
    29101 agagccaaaa aactgggagt tatcggagtt ctggacgcaa tcattgcggg actatttttc
    29161 ggtcgtaccc taggtcagtg gctctacttg ctagttttgt ctagtgtgcc tgttatctta
    29221 catgtatggt ctgctagtga ggactggatg ggcttgttta cctcgtggac agggattatc
    29281 tgtgttatct tggtagcaga aggacggtca agtaactatt tctttggctt tatcagtaac
    29341 ctcatctatt ttgtcttatc ttaccaaaat atgttctatg gggaagtcat gacagcgatt
    29401 ttcttcattg tcatgcagcc agttggcttg tatttctggc tatcagcccg tgtcaatgga
    29461 gcagcagaag aagagaaaac agaatttgaa gcccgtaagc tgactctctg gggctgggtt
    29521 aagtggttag tcttcgctgt cttggtatgg ggaagttttg gcttgattta caagtcaatt
    29581 ggatcagctc gtcccttccg tgactctatt acagatggca ccaactggac aggacaattc
    29641 ctgcaaagtt acctctaccg tgagcaatgg attttctgga ttgcaaccaa tgtcttctcc
    29701 atttacttgt ggtgggttgg tccagatagt ggtaacctgc aaatgtcggt tatgtacttc
    29761 gtttggacta tcaactcgct tgtcggctgg taccaatggt ctaagtccat aaaagaaggg
    29821 caggtggctc gaaatggcta ggcctgctgg tctatcagac aaggaatact acgaaaaata
    29881 tgtgacagag gcagaatttt tagactggta taaaaagcag gacgctccgc gatttgaaaa
    29941 accaagcgtg acagctgata tggtggtcta tagttttgta gaggggagaa tcaagctctt
    30001 gctgattcgt cgtgctgccc acccttgtca acacaagctg tccttggtcg gtggctttat
    30061 tgccagaggt gaggatgcct accagacctg tcagcgtgag ataaaaaaag aagcgggact
    30121 ggatttgccc cgcaatcata tcgagcaact cctaacggtt tccgattctg agcgggatcc
    30181 gcggggctgg atgatgacta ttgcccactt ggtttacctg cctagtcagg ccttggattt
    30241 ggtcaaacca ggtccagatg gacgggagcc gattgttatc gatgtggatt tcaagacttc
    30301 gcagtgttct tatgagggtc aggtcttgac ggcagaggat tttgcctttg accattacga
    30361 gattgtcatg acctctatcc agcggattca gggacggatg aagtggaatc caacctttct
    30421 caatctcctg cagcagccct ttaacatcta cgctgcgaca gacttggtca atcttatctc
    30481 acccgacaaa aaaatcctcc acaataattt cctagccaag tatggagact ttgtggaaga
    30541 agttggagtg gaacgattgc ccaaacgcaa acccagaaag acctatcgct taaaagaaag
    30601 ttcataaaaa gataaagaaa cctccagctc aaactggagg ttttgatata gtcgaatgaa
    30661 ttagctttca gacaaggaac tgaggtgcag gctgtactag agtacggcaa gccgaaagtg
    30721 acgatgtatc aaagttaatt cattcgacta tattatttag caatccgttc ctgataggct
    30781 tctcgtgctt ggtcaaagag ttcttggacc tgctcgatag tctctgctcg ggcaacagca
    30841 ccacggattt tggcagcgcc tgctgaacca cggaggtagt gaggggcaag accacggaat
    30901 tcacgtacgg caatggactc acccttaaga ttggtcaatc gggtcaggtg gtcgaaggcg
    30961 acattgagtt tgtcgtcaaa ggagaggtcg ggtaggactt ctcctgtttc aagatagtga
    31021 ttgatttggt tgaagatgta tggatttccc atagcagtcc gtccgaccat gacagcgtcg
    31081 gcaccgactt cctcgattcg ctggcgggca tcttctacat tgcggatgtc gccgtttgcg
    31141 atgaagggaa tcttggtcag gctgccagct accttggtca aggtctctag gtcaactgtt
    31201 cctgtgtaca tctgctcgcg ggtccgtccg tgcatggcca gggcagctac accgccactt
    31261 tctgcggcca gggcattttc gaccgccagg tcggtattgt tccaaccggt ccgcatctta
    31321 acggtcaggg gaatatcgag gaccgaggtt acttccttga tgatgtggta gatcttgtcg
    31381 ggatccttga gccacttggc accagcttcg tttttaataa ccttgttgac agggcaaccc
    31441 atgttgatat ctacgatatt agccttggtg ttcttctgga tgaagtcggc agcccgtttc
    31501 agtccttcgg cgtcgccgcc gaaaagctgg atagacatag ggtattcgtt gtcgtcgata
    31561 tggagcatgt ggagggtttt ctcgttgttg tagagaaggc ctttttcaga aatcatttcc
    31621 ataacgacca gtcccgctcc catttccttg gcaatggtgc ggaaggccga gttggtcacg
    31681 ccggccattg gggccagcac gcagcgattg gggatttcca catcaccaat catgaaaggg
    31741 gtattgagat tagccattga tgagttcctc caaatcgttt tcgtcaaagt ggtaatgggt
    31801 ctggcagaat tgacaggtga tgtccacgcc cttgtcttct tctttcatct cttgcaggtc
    31861 cgcttttggt aggctggcta gagcatcgag gaagcggtct ttagagcagt cgcagacaaa
    31921 gccgatttcc tcttctgaca ggcgtttgaa gtcgtcgtcg ccataaatgg cggatagcag
    31981 agcttctatg tggttttcag aagccagcaa gcttgaaatg gcaggcattt cttggatgcg
    32041 tttctcgaaa cgggcaattt caacctcggt cgctccaggt aaaacttgga gcaagaagcc
    32101 gccagctacc ttgaccttgt cttcttcgtc caacaacacg ttcagtccca ccgcagatgg
    32161 tgtctgttgg ctgtctgtca ggaaataagc aaagtcctca ccgatttcac cagaaatcaa
    32221 gggtgtcatg gagttgtaag gatgacctgt tccgtagtct gtgataacca ggaattgtcc
    32281 gttccctaca aggggcccaa cgatgacttc gccagtcgct gtccgtttgt agtccaagtc
    32341 aggattttga atgtagccct tgacctgacc tttggtattg gcaacggaga tgatagcccc
    32401 aacagcaccg ctggccaaga ttttcagggt gattttggtg tcgccttttt cattgacagc
    32461 caaaatctga ttggcaatga gggtgcgacc tagagcaacg gtggacgacg ccatggtgtc
    32521 gtgtttttct tgggctgttt tcacggtttc tgtgctatct agcacaaatg cacggaaatg
    32581 cccacttttt gatagtgttt taataatttt atccataact ttaattatag cacaagtgga
    32641 ggtggagcag tagaaaaatg agcctgaaag tcatgctttc aagctcaaaa aatgattagg
    32701 attctttttg ttttttgaca agacaggcgc tcaagaatac gagaccgatt gtgataagta
    32761 caaagcttgt ttctcctcct gtctttggaa ggattttctt ttgactgttg ttggcagtag
    32821 gggtcacggt tgtttgtctc ttgtctgtag cgccagaacg gtcatcagca tttacagctg
    32881 tttgattaga agttgaaaga tttgttggct tgttattgtt ggtcgtacca ttgtcatcac
    32941 tattattgac tgttcctgag tcatctgtct gcttgtcgct tggttctggt tgagttggtt
    33001 cttgtgcctt tgtcacatcc agttgtacca atagtggatc gtggtcagaa gctctgccat
    33061 gttcttccat aaaggctgaa ttgacatgga ccatatcaaa ggcatagcgc tccaacaagt
    33121 tagttgatac tagcatattg tcaagagact gattatttcc gttatagaag taagagaagc
    33181 gatcggatgc atcgtgacga ctaacgaggt tagccattcc tcctgcttct aaaatttcta
    33241 tggttttagt aaattcatag tcattgaagt cgcctagcat aacgatatta gcatttggat
    33301 tttgagctaa gcctgctttt gtaaagtcag cgatagtttg tgctagtatg tggcgttttt
    33361 cttcggactt gaagctgaca ggttggattt ttccgtagag accattgtca ccgcgttttg
    33421 agttgagatg gttggcaagg acaacgactt tctcgccttt gaagacgaat tcagctgcaa
    33481 gtgttttgcg gacagctgcc caagcagggt tggttgggtc gattcggcct aggctgagat
    33541 tgagttcgcc gttttcccat gcaactgcct gtgttgcggt accgattggt ttgtcagata
    33601 ggctgacacg ttttgagttg tagaggaagc cgacgcgaat attgcctccc tcttgtccgc
    33661 cgtctttgtt gttttcagga gcaatgtcta cataggtata ggttggtccg ccggctgctt
    33721 gaatggctgc aatcaggcgt tcagcactct tactagcatc agttgtgccg tcgtttgtag
    33781 caccgttgtt gtcctgcact tcaatcaatc cgataacatc tggagaatgt aggtctgaaa
    33841 caaaggattt ggcaatgcgt tggactttag cgtctgatgt tgacttacta ttagcagaga
    33901 agttttcaat gttataagaa gcgatagtca gcttgtcatc atttgggata atggttgttg
    33961 tttctggttt tgtagctcct tcgtgcaatg ttggaagggt gctatcatct acatagactt
    34021 tataattggt gtaagagtag gtgactggtc cagcgatacg accgatgaaa tagtctcctg
    34081 atttgacgat ttgttttcca tttttcaaaa gaagtggaat gatattggta ttattccctt
    34141 cagggcgaag gttgacgcct ccaacattat ttaaaggaag ttgactagtg gcaggagtga
    34201 catagatttc cttgtttttt agtgggccaa ggatttttgc atcatccacg gcaaccacca
    34261 tgccttcaac ggattcccag aaatcgagcg catcttgttc tggatcaaat tgagccaaac
    34321 cgtcgttgtc aataatatct gctgggatag tccgatcaag accgaggact ataggagatg
    34381 gtacaggtgc agtcccatcc acagttactt cagtagctcg aatttgtgtg atggttaagt
    34441 ctgttttgtc acgttcagca taccctctgc cctggtattc ttctactcgt cctgcgatag
    34501 tgacaagatc tcctaccttg acatttgttt ttaatttgtc agtaaagatg ttaatgccgt
    34561 cagatgtttt ggtgtctcca tctggtgtga catcttgcac gtagaagtta ttggcagagg
    34621 taacgtaggt tactacgaca tttttaagca taacagattg attcaccaga ggtgactggt
    34681 gacttgcacc ttgaatgcga ccgactgtta cagcttcggt ctttcctagt cctgcttccg
    34741 tcgttgcctt ttcaatgact tcaaaatgag agaggtcaaa tggttttaat tgtatttttc
    34801 cgttataggt ggataaaata gcggttagat taatcttatc tcccttgttg atccggttca
    34861 aaagatccgc cgttttgatg cctgttcggc tatctaagcg tacatcaaca gtttgtcctt
    34921 cggagtcagt tacggtaaag gtagaatttt gatagctatc gctttgaata tctccgacag
    34981 tcaagttttt tagagaaact agtgttgcct gtgcttgcgt tgctaactga gcgatgtttg
    35041 tttctgtaat aggagtgttg aaattctcag agatagcctt gtggttgcta actgtggtta
    35101 gttggagttc gcctttatac tcacccaatg ttcctgttaa ttggactgta tcaccaagct
    35161 gatagcccaa ggcagcccct gggtagatat agataccagc accgtcagaa ccctggaggt
    35221 aaaaaccatt tcctccccaa ccgtttacta ggctaataat tttaccagaa actgtgtact
    35281 ctgttccttg tgctccggaa cgaacagtag caatcggtgt agaaacaagg tcacttggtt
    35341 tggtagctgt attttcactt gttggtggct gaacattagt agaagatgaa tctacgattt
    35401 caatcgctgt agctggtttg atcccagctc gcttcatgta tgtatcgcta gttcctgtga
    35461 tgcgaaccaa ttttccgact agttcgggat gattaacgag gttgaattgg ctacgaagag
    35521 gttcttttag ttgaataggg atagtgtcgg ctgccgcagt atccatgctt gcaccaaggg
    35581 ctagatttgt tttgttagtg gcgtcaaaag cagttccgct actattaagt ggtgcaataa
    35641 gatagccttg tatcgtgact tccgacttgg ttattgcaag tgacggataa ttctctatcg
    35701 ccacttcagt agcgtgcaca gatggtatgg gcgaagtagc aagactaagc aagagtccgg
    35761 ctgttaccgc tacagaacct acggtataga agagagaacg gtttctaatc ttcatataaa
    35821 actccttttt gttatatatg gacgtcataa aaacgtctgc agttgttata tacatatagt
    35881 ttaacagaaa atgagtcgtt tggaatactt ttttagatat aatacggata tttttaaaaa
    35941 ttcaaatgaa atggtaaaaa taaattggaa aacgaaacaa aaaacgaaca aatcggttgg
    36001 gtgatgggaa ttggttcaca aaaaaagcac cgagatataa atgattttct tggtgccata
    36061 ttcattttgt ttaacattag ttgggagttt ttgtgaagga acaaaagggc aaagcagata
    36121 tatagtcctt ttgttgactt tttttacttc ggcgcaagag gaactccgtt tctggatttc
    36181 ccgcttcaaa caatccactg gtttgtttga actcgctttg ccgtacttta tagagaggca
    36241 agtcttattt ccaactgagg ctgggcaaaa agcccaggac cactactcag agttcgtgtc
    36301 aacatctcag cgcagtggtt gattgggttt aacagtccag tggactgtta aaggttggag
    36361 ataggatttg cgaagcaaat ctcagcaatt cgagtttcac actccaaatc tggcctctac
    36421 gactgatgcg aacagagttc gccttatctc caacctcaaa ctgtctccca gacagtttga
    36481 gctgtgcggg ggtgggagtg aaacagtctg gggatagact gtttcagctc aacaacttaa
    36541 aacgaaaact tgttgacgaa ctctttttat cttggtcgag ttctttctca ctcccagcaa
    36601 cctgcagcta ttgaggcgaa cgtgctttgc cctatcccca gccttcaaaa gttccacgaa
    36661 cctttgaagt aagcactaac aaagtaagga aaatggaaaa ctatagctct ggaaataatt
    36721 ttttcaaaat cttcggcgta atgctctgac caggtcctgc gtaagtatag gtgtgcaggt
    36781 acatggcagg gttaaagttg agttcgatgc aggtgcagtt gggcttgttc ttgcttgctg
    36841 gcttggtgcg gtcggggata atcaagtcca caccgcaggc ccaagctccc atagcagttg
    36901 ccatatcggc ggccagttgc ttgtaggact cgtccatctg gtcggtcata tcgatggagt
    36961 caccacccgt tgagatattg gaattgccac gcagaaaggc ttgaatgccc tctggcaaaa
    37021 ctgtatcggg tgtgtagccc tgctgttcta acataagcag ttcgatgtcg cccaagttaa
    37081 tgatctccag cggtgaacgg tggtcacgcc ctcgcagtgg atcttggttt ttttgctcta
    37141 ctaattcacg gatggttgag ctaccgtccc ccacgacatt ggctgcgacg cggagcagga
    37201 cagcctcgca cttgccatca aggatgaaga aacggtactc ggttcccgcc acgaattcct
    37261 ccacaagcac atggctatct tccgaaaagg cgatgtccag ggctttttca tagtccgcaa
    37321 ggctggctgg ctcctggaag atagagatgc caaggccgaa gttggtagat tttggtttga
    37381 taacaatagc agagctggct acctgtccgt aataccgcag ggcatctgtc ttgtttgcaa
    37441 attcagctcc cgcaggtact ggaaaaccag cctggtccaa aatcttcttg gtcacgacct
    37501 tgttggccat ggctagagga atgacgtaat tgtccttcga agtcatgttg ccatttttga
    37561 tgtactcgac atggttacca tgccagagtt tgagaaactg gtcttcttca tctaaaatct
    37621 ccacattcaa gcccaactga atggcgtcaa agagaatcat ctgggtggag agttccatgt
    37681 tttcatagcc cttgagggcg taaggggctg tccaagcata gtcatgaaag acttgtccct
    37741 gctgctgacc gaacttctct agagacccat cttcaacttg ctctgctatt tttccggaaa
    37801 gggtcaaacg agggtcttga agtgctgcct ccgcatgtgc aatgagttgg ccatagtagt
    37861 catccagtcc aaagtgtaga actatggctt tcatggccgt caagattggc gtggcatcgg
    37921 catcgttagg tagaggtgtg tgtgggtgac tgagggcgat gagattgtta agtttatctg
    37981 cttttgctaa ggtgttatca acatctgtca gttggtctaa ccaaagaaga gataagataa
    38041 agaggtggac tgtatcaagg gtttcttgac taatagcaag cgggtcatag gggttcaggt
    38101 cgaaggaacg gaattctaaa taagaaatcc ccttgctgag gtagtcacgg ctagtctttg
    38161 ctccacgtag tcggacagaa gagtaaaatt ccttctcagc ggatagttga ccagattgga
    38221 cagcttgttc gatgtcggcc acgtactgtt ggagagaaga aaaggaaata tgaacatcat
    38281 cggagttgac atagccgtac ttggaattac gaatggaacg gacgcaaccg acttccgtgg
    38341 ttaggaatcc tttttcggct aggctacttg ctccatatag gtaggttaag agccaacgat
    38401 agttgaggaa gttttgtgct aatttgaggt agagggtatt tttgaatgcg ataaaatctg
    38461 tttcctcgct taattcaaaa agttgttggg ttaagtcttt tcctaattca aaattataat
    38521 ggatgccaga catagactgg acgagcttgc catagcgttc ccccaaaccg acacgatatt
    38581 ggtactcata gtcgctgtct agttgagcaa tttggatttc ctcttctgaa atcacaggtg
    38641 gcatggatag gggccagagg tattcttgct tatccataga acgtgcggca acatctgtaa
    38701 tggctcccag taggcgacgc gcttccttag tcgactgagc gattggtgta atcaactcta
    38761 actgtggctc gctgtagtct gtctggatat aggggtggaa gctacgagaa cccagtttat
    38821 gaggatgggg agtttgtgca actctgtggt ttgaattgat gcgaagtgat tcgcgctcaa
    38881 ttccaaaagt agcctgaaga atggggctgt ttggtgataa tttttgtaac atatctaagt
    38941 cctttatatt tctgtcaaag tcttattcca attttatcta gattggagag caaatacaaa
    39001 tttctgctac gtttctggtg aaaaatgtac tgaaaatcac tgaaagtaaa caaagacttc
    39061 ggatttctct gccaaaagca tatttacaca tgtctacctc gtgtgtttaa tcggattttt
    39121 ttaaaatata cttttaaaaa aataaaaaca ttcgatatat atgaaattat tggaatttct
    39181 tgaaaaaatg ttcgtttttt gatagaatag tgtgtaaaac gaaacttgca ggtgagattc
    39241 ctgccagaag tagaagaaag gctgggctgg ttagccagtt cagagaaaga gtgaatatga
    39301 catcagtagt tgttgtagga acccagtggg gcgacgaagg taagggtaaa attacagact
    39361 tcttgtctgc taatgctgaa gtaatcgcac gttatcaagg tggtgataac gctggacaca
    39421 ctatcgttat cgatggcacg aagtataaat tgcacttgat tccgtcagga atcttcttcc
    39481 cagaaaagat ttctgtaatc ggtaatggtg tggttgtcaa tccaaaatct ttggtgaagg
    39541 aaatcaacta tctccatgat tcaggtgtga caacagataa tttgcggatt tcagaccgcg
    39601 cacatgtcat cttgccttat cacatcaaat tggaccagtt gcaagaagag tctaaaggtg
    39661 agaataagat tggtacaacc aacaagggta tcggtccagc ctacatggac aaggctgcgc
    39721 gtgttggtat ccgtattgca gacctcttgg acaaggagat ttttgcggag cgtttgagaa
    39781 caaacttggc tgagaaaaac cgcttgtttg agaagatgta tgagtcaact ccgattgagt
    39841 ttgacgaaat ctttgaagaa tactatgctt acggtcaaga aatcaaaaag tatgtaacag
    39901 acacatctgt tattttgaac gatgccctcg accaaggcaa gcgtgtcttg tttgaaggtg
    39961 cgcaaggagt tatgttggat attgaccaag gtacctatcc attcgttact tcttcaaacc
    40021 cagttgctgg tggtgtgacg atcggtagcg gtgtcggccc aagcaagatt gacaaggttg
    40081 ttggtgtatg taaggcctac actagccgtg ttggtgacgg accatttccg actgaattgc
    40141 acgatgaaat cggagaccgt atccgcgaaa tcggtaaaga gtacggtacg acaactggcc
    40201 gtccacgccg tgtcggttgg tttgactcag tggtgatgcg ccatagccgc cgtgtgtcag
    40261 gtattaccaa cttgtccctc aactcgattg acgtcttgtc aggtcttggg accttgaaaa
    40321 tctgcgtggc ttatgacttg gatggtgagc gtattgacca ctacccagca agtttggagc
    40381 aactcaaacg ttgcaaacca atctacgaag aaatgccagg ttggtctgaa gacatcacag
    40441 gtgtacgtag cctggatgaa ttgccagaag cggctcgcaa ctatgttcgt cgtatcagcg
    40501 aattggtagg cgttcgtatc tcaaccttct cagtaggacc tggtcgtgaa cagaccaaca
    40561 tccttgagag tgtatggagc gcaaaatagt gtgcgaagac tgaacgaaag ttcggtcttt
    40621 ttctgtttgg tcgaactttg ctttcttgtc cagaatttga tataatcatg cttatcaaac
    40681 aataaaagag gtaaacaaat ggaaggttta tttttgccac ttgtcatggt tgccatgatt
    40741 ggttttatgt tctattcgca acgtaaacaa caaaaacaac gtcaagaagc actaaatcaa
    40801 atcaaaaaag gtgatgaaat tgtgaccatt ggtgggctat ttggtattgt cgatgaaatc
    40861 gatgataaga aggttgttct cgatgttgat ggtgtttact tgacatttga acgtggtgct
    40921 attcgtgatc gtgtagcaag ttcagatgct acatcggcag ttgttgaaga agtggcagtt
    40981 gaaacgccag aagcaactcc agaatctgca attgaagaat aaggatgaga aaccgaggga
    41041 aaccttggtt ttctcttgtt ttagccctgc ctgatttttg ctatcctagg taaaaatatg
    41101 gtataatgaa acgattatac aataaggaaa ataccatgtt taaatttaat tttaaaaaga
    41161 aagaaagcat tgaaacagca attgaagtcc ctaagcatat cgctgtgatt atggatggca
    41221 atggccgttg ggctaaaaag cggatgcagc cacgtattat ggggcataag gctggtatgg
    41281 atgcactgca aaaggttacc aagacggctt ctgatttggg agtaaaggtg ttaactgtct
    41341 atgccttttc tacagaaaac tggtctcgac cggaaaaaga agtgaagttt atcatgaatc
    41401 tacctgtgga gttttatgat aagtatgttc ctgaactcca tgccaataat gtcaaaattc
    41461 agatgattgg tgaccattca aaacttcctg tcccgactct caatgcgctt tataaagccg
    41521 aacaaaagac aaagggtaac actggtttga ttctcaattt cgctcttaac tatggcggtc
    41581 gtgatgaggt gactcgcgca gtcaaggcta ttgcgcaaga tgtcttggat gccaagttca
    41641 atcctggaga tattgatgag aaattaatag cggattacct ctatacagga accttatcac
    41701 caacgttacg cgatcccgat ctagttatcc gtacaagtgg tgaacttcgt cttagcaact
    41761 tcctgccgtg gcaaacagcc tacagcgagc tgtactttac agatattgct tggcctgatt
    41821 ttgatggaga agcacttaaa ttagcgatta aagaatacaa ccgtcgccac agacgttttg
    41881 gcggtgtata aggagaaggt atgacaaacg atttacagaa acgagtgata tttggcggca
    41941 ttgctctagc tattttcatt ccctttttgt tggctggtgg gatggcattt caattttttg
    42001 taggcctgtt agctattatt gctacggctg aattgattaa aatgtatcgc ctagcaccaa
    42061 attctatcga gggtgtctta gctatgttgg ccagtttagt cttggctctt ccgctccaga
    42121 attatttgac atttttaccg acggatggaa attacacagc ctacgccatt gtagtatttt
    42181 tactgcttgg ttcgacggtt tttaatatcg gtcattacaa ctattccgat gttgtctttc
    42241 caattgcatc aagtttctat gttggtatcg gctttcatag tttgatactg gctcgtatgg
    42301 atgggctcaa taaagtcttc tttgccctct gtctagtctg ggcaacagac attggtgcct
    42361 acttaatcgg tcgtcagatt ggtcggagaa aattattgcc gaaggtttca ccgaataaaa
    42421 cggttgaagg ctttgtaggc ggtattcttt ctgcggtggt agttgctatc attttcttga
    42481 ttgtcgataa gtccttgtta gcgggctatt catttggcat gatgttgttg ttggttgtga
    42541 ttttctcgat attctcacaa ttcggagact tagtagaaag tgctattaaa cgccattttg
    42601 gtgtgaagga ttctggaaaa attattccag gacacggtgg tatcctggat cgttttgacg
    42661 gtatgatttt tgtctttcca atcatgcact tctttggatt attttaagga ggtactatga
    42721 agggaatttt agcatttata tttatatttg gtgtgattgt ggttgtccat gaatttggtc
    42781 atttctactt cgccaagaaa gctggtatcc ttgtgaggga atttgcgatt gggatgggtc
    42841 ccaaaatttt cgctcataca ggtaaggatg ggacacttta caccatccgt atccttcccc
    42901 taggtggcta tgttcgaatg gctggatggg gtgaagataa gacagagatt aagacaggta
    42961 gtcctgccag cctcagcctg aatgaagctg gtgttgtgac gcggattaat ttgtctggta
    43021 agcaactgga tagtctcagt ttgccaatga atgtaaccag ttttgacttc gaggaaaaat
    43081 tggaaatcac aggcttggtc ttggaagaaa gcaagaccta taaggttgat cacgatgcca
    43141 cgattgtaga ggaagatgga acagaagttc ggattgctcc acttgatgtt caatatcaaa
    43201 atgcgacagt ctggggacgg ttgatgacca actttgcggg tcccatgaat aactttattt
    43261 tagggattct agtatttatt cttttgttct tcatgcaagg aggggttgcc aacccatcaa
    43321 gcaatgcagt aagtattact gaaggtggtg ctttgcaggc tgcaggtgtt gtcacagggg
    43381 acaagattct ttcggtcaat gggaacacga cagacagcta cacagaagtt gcaacgatta
    43441 ttagtaaagc agcaacagat gcaacaacag ccccaagctt tgatttggtc gttgagcatg
    43501 atgggaaaaa ccgtcatgtc tccgttactg ctgaacaagt agatggggcc tatcgaattg
    43561 gtatttcacc gattttgaaa actggtttcg ttgataaaat tgtcggtggt ttccaagaag
    43621 cgggagcgac agctcttagg gttgtaacag ctttgaagaa tctaattgca aactttgatg
    43681 ttaagcagtt ggggggaccg gttgctattt ataaagtaag ttctcaagca gctgaatttg
    43741 gtttggtttc tgtattgggg ctaatggctg cgctctccat taaccttgga atcttcaatt
    43801 tgattccaat tccagccttg gatggtggta agattgttat gaatattctg gaagccattc
    43861 gcagaaaacc actcaaacca gagacggaat cttatatcac tctagcaggt gtagccgtta
    43921 tggtggttct tatgatagta gtcacttgga atgatattat aagagtcttc ttctaagata
    43981 atttgaaatt tatgaaagga attgaatgcg tgctaaacgc aggatattct gaaaaatacg
    44041 aaaggaattg aatgcgtgct gaacgcagga tattctgaaa actaagaaag gaactgaaca
    44101 cgggctgaaa cctgtgcatt cctgataaac aaaagatgaa aggaattgaa tgcgtgctaa
    44161 aaactttacg aaaaagataa acctgcctag tgccaaaggc actgcgttag gtttcctatt
    44221 ttcgtttcgt tttcttaacg cacttatatc ttaactatga aacagtctaa aatgattatc
    44281 cctactttgc gggagatgcc ttcggatgcg tcggtaatta gccacgcttt gatgttacgt
    44341 gcgggctatg tccgtcagat ttctgctggt atttacagct acttgccttt ggctaaccgt
    44401 gtgattgaaa aagcaaaaaa tatcatgcgt gaggagtttg ataagattga tgccattgaa
    44461 tttttggcac cagccctctt gtccgcggat atttggcgtg agtcaggtcg ttatgaaacc
    44521 tacggtgacg acctctacaa gctcaaaaac cgtgaaggtt ctgactttat cttgggtcca
    44581 acccacgaag aaacggtgac gctattggcg cgtgatgcgg tccagtctta caagcagttg
    44641 ccgcttaaca tctaccaaat ccagccaaaa taccgtgatg aaaaacgtcc tcgtaacggt
    44701 cttctccgtg gtcgtgagtt tatcatgaaa gacggctaca gcttccatgc tagttatgag
    44761 agcttggacc agacctacga tgactacaag gcggcttacg aggcgatctt cactcgtgca
    44821 ggattggagt tcaaggctat tatcggtgac ggtggtgcca tgggtggtaa ggatagccag
    44881 gagtttatgg ctattacacc agaccgtacc gatctcgacc gttgggttgt cttggacaag
    44941 tcggttgctt cttttgagga aatcccagag gatgttcttg aagcgttcaa ggcagaactc
    45001 ttggcttggt ctgtgtctgg tgaagacacc attgcctact caagcgaatc aggctacgct
    45061 gccaacctag aaatggcaac tagcgaatac aaaccaagta cagcagttgt agttgaagaa
    45121 gacttggtta aagttgctac accagatgcc aaaaccatcg acgaagttgc tgccttcttg
    45181 aacgttgcgg aagaacaaac tatcaaaacc atgctcttta tggctgacgg agagccagtt
    45241 gttgccctcc ttgttggcaa tgatcaggtc aatgatgtca agttgaaaaa ccacttggca
    45301 gcggatttct ttgatgtagc aagcccagct gatgcagaga aagtcttcgg tgcaggcttt
    45361 ggttctcttg gcccagttgg tttaccagaa aacatcaaga ttattgcaga ccgtaaggtt
    45421 caagatgtga agaacgctgt agttggtgct aacgaagatg gtttccacta cacaggtgcc
    45481 aatgcagggc gtgatttcca agtgactgag tatgtggata ttcgcgaagt caaggaaggc
    45541 gagccttctc cagatggaca cggggttctc aactttgccc gtggtatcga gattggtcac
    45601 atcttcaaac tcggtacacg ctattcagac agcatgaatg ccaacatctt ggatgagaat
    45661 ggtcgttcta tgccgattat catgggatgc tacggaatcg gtgttagccg tctcttgtca
    45721 gccgttcttg aacaacacgc tcgcctcttt gttaacaaga caccaaaagg cgaataccgt
    45781 tacgcttggg ggatcaactt ccctaaagaa ttggcaccgt ttgatgtgca tttgattcca
    45841 gtcaatgtca aagatgaagc ggcgatggaa ttgactcagt ctatcgaagc aagtttggtt
    45901 ggtgcaggct atgaagtatt aactgacgac cgtaatgagc gtgtcggcgt taagttttct
    45961 gacagtgact tgattggctt gccaatccgt gtgactgttg gtaagaaagc agctgatggc
    46021 atcgtcgaag tgaaaatcaa gggtacaggt gatactgtag aagtccatgt ggaccaactc
    46081 cttgaaacct tgcaaatcct tacaaaagaa aatgaataac agaaaaagac ttggttgatg
    46141 atagccaagt ctttgttgat aaaatgggag tggttctagt cacccaagcc tattttttat
    46201 atagtcgaat gaattagctt tcagacaagg aactgaggtg caggctgtac tatagtacgg
    46261 caagccgaaa gtgacgatgt atcaaagtta attcaaatga ctataacata tttagtagga
    46321 tgttatagag gatagctcct gcgacacctc caagaattgg gccagcaact gggatccagc
    46381 tataagacca gtcagaatct cccttatttg gaatgggtag gatggcatgc atgatacgag
    46441 ggcctaaatc acgagcagga ttgactgcgt agcctgtcgt tgatcctagt gagaagccga
    46501 ttgcaaatac gataagtccc acgatgattg ggccgaggcc agcgctgaca ttgttaggac
    46561 caattgcaag gattcccatg actaagacag ctgtaccaag agcttcgccg agcaagtttg
    46621 aagctgtgtg acgaagagct ggtgcagtag agaaggtagc aaggattgtt ccgctatctt
    46681 ttgtttcctg ccaatgtggg tagaaatgga gatagaggac gatggatgcc aacattgctc
    46741 cagcaaattg ggcaagtagg aagcctggga cttgagccca tggtagggag ccgatagatg
    46801 ccattgcgat agtgacagct ggatttaggt gggcaggtcc aaaaattcct gatatgtaga
    46861 cggcaattgt tacagcgatg ccccatccta gtacgatggt cagccagccg ctattttcag
    46921 ccttggtttt agagaggatg caggatgcaa ccacaccgtt tccaagtaat actagtatga
    46981 aagttccaag taattctcca atcagattca taatgacctc ctatttattg attgtcagtt
    47041 cttttaattg ttcaagaata gtctccaagt cagctccagc ctgtaagaga gctgcggcag
    47101 tatagcaacc ttcgacgact ggtacggttt ggataagaat ttgtttgtct gtaaaatcgg
    47161 cagcaagttc catgttcatt cgggcgctac ctaggtcgaa aaaggcaagg agggtatcag
    47221 cagggtttgc ttcaactcgc tcttgtacga tttcaaagct ggtaccgatg ctaccatctt
    47281 ctagtccgcc acagtaggtg atgggaatat cttttgcgac ttcagatact aggtcgatga
    47341 ttccctgggc caaatttttt gaatgggata cgagtaagat accaaccttt gtcatagtcc
    47401 tgcctccaac atggcttcaa agagaagtcc agatgagaaa gagcctggat caatgtgtcc
    47461 aatagacctt tccccaacat aggacgcgcg tcccttggtc gcagcaatat cctttgtccc
    47521 ttgaacggct ttatggataa tttccgttgt cagctggctt tctcttaggg cagaaataac
    47581 aggaatccac acgtctacca tggttttctc gcccaattca gccttgccgc gtttttggat
    47641 catgtccaaa cctgcttgaa gcgtatctgc caaatcagcg ccagcttgac tggctttagt
    47701 aatacccata aaggcagagc cgtagagagg accggaagca ccgccaacct tgctgagaag
    47761 ttgcatggca accaccttaa agacttggtc agcagtctca tagtcttttt cagatagctc
    47821 ctgcatgacg gcagccattc ctcgcgccat gttgcctccg tgatctccat ctccgattgg
    47881 agaatctagc tcactcaaat aatctttgtt ttcttgaatt tttttgttga atgtttccat
    47941 ccagagcaat gcttgttttg catccatagt ctttctccta ccaagctacc acttcaacag
    48001 gggcctgcaa agcgtcaatc cagcttgggt cttctagttt gattaaggtc aatgacagtc
    48061 cagccatatc aatcgatgtc atgtagttac cgattttctt gtagtcaacg accacacctt
    48121 ttttagccag caattgagca acgtcgttgg caaagacata ttgttccatg agaggagttg
    48181 cgccgagacc gttgataagg acaccatagt gttcgccatc ttgaatatca aagccttctg
    48241 ccaatttctc aaccaattct tcagccaatt tagcagatgg ttggagtttt tctttcttat
    48301 agccaggttc accatggata ccgacaccgt attcaaattc atcctcttca aggacgaagc
    48361 ctggtttgcc cacttctggt accgtagcac ctgatagggc taagccgata gtcttgattt
    48421 gtgggacgag acggtcagcc agttccttga tttctgctag agatttgcca gttgttgcag
    48481 cgtgaccgag aatcttgtgg acaaggatgg taccagctac accacgacgg ccttgggtat
    48541 agagactatt ttccacagcg atgtcatcgt ccacgatgac actggccact tcgatgccct
    48601 ccatttccgc caattcttgt gccatctcaa agttcatgat gtcgccggag tagtttttga
    48661 tgaccataaa gacgccagct ccctcgtcag cagccttgat ggcttctaag atttggtctg
    48721 gtgttggtga ggtaaatact gcgccacata cagcggcgga caacatgccc ttgccgacaa
    48781 agcctgcatg agaaggttcg tgaccagagc cacctcctga gatgatgccg actttaccag
    48841 ttttttcagc cttgcggata atcacatcaa atccatcgat acgatcgaca agatcttggt
    48901 gcataaagac aagtcctttt aacatttcat caacgacatg ttcgggttga ttgataattt
    48961 ttttcatgag aaaactcctt tgttattgtt tgcgctttca ttttacattg tccaaaagaa
    49021 aaaaggaagg gacagattgt ccaaagtgtc ccttttgttc caaagaaaag catgataggg
    49081 tataataaag aggaagcgaa aggaagtgac aagtgacata tgccaacctc gttgattaca
    49141 aagaaaagga ttgcaaaggc ttttaaaaaa cagctatccc aaaaatcctt tgataaaatc
    49201 tcagttgtcg acatcatgca ggaagcaggg attcgtcgtc aaacctttta caaccatttt
    49261 ttagacaagt atgagttgct ggactggata tttgagacag aattgcagga gcaggtaact
    49321 gacaatctga cctatattag tggaatcaag cttctggatg agctgttgta ctacatcgag
    49381 agtaaccaat ctttctatag gcaactcttt gagattaagg ggcaaaatga ttttgtcagc
    49441 tatttagaag gctactttat ggttttgatg gagaaaatta tcaacgagta ccagctccag
    49501 gagggtaaaa tgatttccca acaggataag cattttttga ttctctatca tgctaatgct
    49561 tggattggac tgataaagga aggactgaca cagtcacctt gtcagattca cagttattgg
    49621 aagcaaatgg tggctcttgt tcgcgagtcg tttgtgattt tgtaggagtt aatatatggt
    49681 gtttattaga aatagacagg caacaattat tgaagattat ttggatggtt tctgtcgaac
    49741 taatcctaaa gtgaagaaag tgaagggact tccgattgtg cttcagaaag tgcaggatga
    49801 ccatttagtt ccaattattt caggtggtgg atcggggcac gaaccggcac atattggatt
    49861 tgtcggaaag ggtatgctga ctgctgcagt atatggtgaa atatttacgc ccccaaccgc
    49921 tgaagaagta tttgcagcca ttcgcgcagt agataagggc aagggagttt ttctgattgt
    49981 aaaaaacttt gaggcagacc ttatctcttt tagaaaagcc atcgaattgg ctcggcagga
    50041 gggaattgca gtcaagtaca ttgtatccca tgatgatatt tcggtcgata ctaagaataa
    50101 tttccgtatg cggcataggg ggcttgcagg gaccattcta cttcataaga tattaggagc
    50161 tgctgccctt actggctata gcctagatga attggaacag ctgggactgt ctctggcaac
    50221 agagattgcg accatcggtt ttgcaaccaa gtcggcctat ttgcccaatg ctgccctgcc
    50281 tttgtttgat ttggaggagg gacaaatatc ttacggtatc ggtatccatg gagaagaagg
    50341 ctatcggacc gttcgttttg aatcctctga gcagttagcc aacgaaattg tgaacaaatt
    50401 aaaattaaaa ttccgctgga aggaagggga agactatatt cttctggtca ataatcttgg
    50461 atctatgtcc gaggttgagc aaggaatctt tttgaacgac atttgccaat ttctagagct
    50521 agaaggttta cagctttcct atgtaaaaac aggtaaattt atgaccagtc ttgatatggg
    50581 aggaatttct gtcaccctct gtcgtgtcaa ggatcgaaaa tggctggact ttttagaagc
    50641 accgacagaa gctgcggctt ggtaggctac caatttctat agaaatcaga tctctcagga
    50701 aatgaacctg agagtttttt cttcctgact caggaaaaaa tacctcattt atggtaaaat
    50761 gtttctaacg aactacaaag gaaataagta tgtcagataa gtttcaactc ttgctacaac
    50821 aaatcggaat gcctcttgat gcacgacaat caggggcttt ttcgactgca acgattgaga
    50881 aagtagtctt gcacaaggtt agcaaacttt gggaatttac cttccgtttt gaaaccccct
    50941 tgccgctcat ggactatcaa ctcttcaagg ctcgtttggc gacggagttt gaaaaggtgg
    51001 gcaataagat tcaattttct attgtcagcg atgcggaggc ttttgaagct ggtttggtag
    51061 aagcatatta tccagaagct tttacggaag atttgtgcca gagtgcaggc ttcaaggccc
    51121 tttttcagcc attagaggtg gcttatagag atggtgtttt gtggataaaa ggtcctgaaa
    51181 ccattgacac agatcatttt cggaagaatc atctgccaaa tcttgtggag caatacaagc
    51241 gatttggttt tggtaatctt gcggtggaca tccaagtctg tcaggaaatg acgcagcaac
    51301 aagcggagat tttccatgcg caaaatgcag aaatttacca gcaggctaat gaagaaaact
    51361 tggcggccct tgaacaacta gctcaaatgg cgccaccacc agaggcagct cagccatttg
    51421 tcccagaata taagaaaaat cgtcctgcca aggtcaatat cgaaaaggct gagattacgc
    51481 ctatgattga ggtggatagc gaggagaatc ggattgtctt tgagggactg gtctttgagg
    51541 tcgagcagaa gacgaccaag acggggcggg tcattatcaa ctttaagatg accgactata
    51601 cttcgtcctt caccttgcag aagtgggcta agaatgagga agaggctcag aagtttgata
    51661 tggtcaaaaa gggaaactgg ctgcgggtgc gaggcaatgt ggaaaccaat aatttcactc
    51721 gtgatttgac tatgaacgta caggaagtcc aagaggtcaa gaaggaaatc cgcaaagatc
    51781 tcatgcctga gggtgagaag cgggttgagt ttcatgccca taccaacatg tccactatgg
    51841 atgccctgcc tgccgttgag gacttggtgg cgcgtgcggc agcctgggga cacaaggcgg
    51901 tggccattac cgaccacggc aatgtccagt ccttccccca tggctatcat gctgctcgta
    51961 aagctgggat taagcccctc ttcgggatgg aggctaatat tgtcgaggac tctgtgccca
    52021 ttgcttacaa cgaagcagat gtagtcctgt ctgatgccac ctatgtggtc tttgacgtgg
    52081 aaacaaccgg tctgtctgca gtcaacaatg ccctgattca gattgcggcg tctaagatgc
    52141 acaagggcaa tatcattgca gaatttgatg agtttatcga tccaggtcat ccgctcagtc
    52201 aatttacaac agatctgaca ggaatcacag atgagcatgt gcgtggttct aaacctctag
    52261 aacaagtcct gcgtgagttt caagatttct gtcaagattc cgtcatggtt gcccacaacg
    52321 cgacctttga cgttggcttt atgaatgtca actatgaacg agcaggctta cctattatta
    52381 gccagcctgt cattgatacc ttggaatttg ctcgtaacct ttaccccgac tttaagcgtc
    52441 atggtttagg tcctctcacc aagcggtttg gtgttgctct tgaacaccac cacatggcca
    52501 actacgatgc ggaggcgaca gggcgcctgc tcttcatctt cctaaaagat gctctggaga
    52561 agcacaattt gaccaatctc aaccagctaa atacggagct gattgcggag gattcctata
    52621 agaaggctcg tgtcaagcat gccacgctct atgtgattaa tcaggttggt ttgaaaaata
    52681 tgttcaaact ggtctctctt tccaatacca agtattttga gggagtccca cgtattccac
    52741 ggaccgttct caatgcccat cgtgaaggtt taatattggg aacagcctgt caagagggtg
    52801 aggtcttcga tgacttgctc tccaaaggga tagatgaagc ggttaaaacg gcagcctatt
    52861 atgattttat cgaagttatg ccacctgccc tctatgctcc catgattgcc aaggagcagt
    52921 ttaaggacat ggcagagatt gaagagacta tcaagcagtt gattgaggta ggacgtaggg
    52981 caggtctgcc tgttctggcg actggaaatg tccactacat tgacccagaa gaggaaattt
    53041 atcgggaaat cattgttcgt gctctaggtc aaggggcacc gattaactgg accatcggaa
    53101 atggtgaaaa cgctcagcca gcaccactgc caaaagccca ctttagaacg accagcgaga
    53161 tgttggacga gtttgcattc ttgggagaaa gtctggcccg tgagattgtc attaccaatc
    53221 ccaatgccat gctggaccgg tttgaagatg ttcaggtggt taagaccgac ctttatacgc
    53281 cttatattga gaaggccgag gaaacggtcg cggaattgac ctatcagaaa gcctttgaga
    53341 tttatggcaa ccccttgcca gacattatcg acctgcgaat cgaaaaggaa ctgacttcca
    53401 ttttaggtaa tggattcgcc gtgatttacc tggcatcgca gatgttggtg caccgttcca
    53461 acgagcgggg ctacctggtg ggttcacggg ggtctgtcgg ctccagtttc gttgcgacca
    53521 tgattgggat taccgaagta aaccctatgc ctcctcacta tgtctgtcca aattgtcaac
    53581 acagtgaatt tatcacagat ggttcctatg gttcaggttt tgacctgcca gacaaggatt
    53641 gtgaaaagtg tgggaccaag tataaaaaag atggacagga cattcctttc gagaccttcc
    53701 ttggtttcga tggagacaag gttcccgata ttgacttgaa cttctcaggg gatgaccaac
    53761 cgtctgccca tttggatgtt cgggatattt ttggtgaaga aaatgccttc cgtgcaggaa
    53821 cggttggtac ggtagctgcc aagactgcct atggttttgt ccgaggctat gagcgggatt
    53881 atggcaaatt ctatagagat gtggaagtag aacgcctggc tgctggagca gctggtgtca
    53941 aacgaacgac tggtcagcac ccagggggaa ttattgtatt tcctgactac atggatgtct
    54001 atgactttac ccctgttcag tatcctgccg atgatgtgac agccagctgg cagaccaccc
    54061 actttaactt ccacgatatt gatgaaaacg tcttgaaact cgatattctg ggacacgatg
    54121 atccaaccat ggttcggaag ttgcaggact tgtcaggcat cgatccgcaa actattcctg
    54181 ctgatgataa gggggttatg gctctttttt ctggtacgga aattctgggt gttaccccag
    54241 agcagattgg gacaccgaca ggtatgctgg gcattcctga atttggaacc aactttgtac
    54301 gggggatggt ggaggaaacc aaaccgacca ctttttcgga actcttgcag ttatctggtc
    54361 tatcgcacgg tacagacgtt tggttgggca atgcccagga cttgattaag gcaggaattg
    54421 ctaatctatc tactgttatc ggttgtcggg atgacatcat ggtttacctc atgcacgcag
    54481 gtttgccacc taagatggcc tttaatatca tggagcgggt gcggaagggc ttgtggctca
    54541 agatttctga ggaagagcga aatggctaca ttcaagccat gaaggataac aaggtgccag
    54601 actggtacat cgaatcctgt ggaaaaatca agtacatgtt ccctaaagcc cacgcggctg
    54661 cctacgttat gatggccttg cgggttgcct atttcaaggt tcatcaccca ctttactact
    54721 attgcgctta tttctctatt cgggccaagg catttgattt ggcaaccatg tccggtggct
    54781 tggaacgagt caaggctaag atggaagaaa tcgccctcaa gaagaaaaac aatgaagctt
    54841 cgaacgttga gcaggatctc tataccacac ttgaattggt aaatgaaatg ctggaacgtg
    54901 gcttcaagtt cggtaagttg gacctctaca aatcccatgc gaccgacttt ctgattgaag
    54961 aagataccct catcccacca tttgtcgcta tggatggtct gggagagaac gttgccaagc
    55021 aggttgtggc ggcgcgtgcc gaaggagaat ttctctccaa gacagaactc cgtaaacgtg
    55081 gtggattgtc aggtacgctt gttgaaaaaa tggatgaaat gggcatcctc ggtaaaatgc
    55141 cagaggataa ccagttgagt ctctttgatg atttgtttta aaaaaggaaa tgatgaaaaa
    55201 aagaaatatt ttagttgagt tattaaaagg aattggtcta gtactgcttt ctactatctt
    55261 gtggacattg atcttatctt ttgccacacc ggaatcatca tcaggtattt acgaaccttt
    55321 tccaggtttt actgtgattg ctggtatact aacgagtttc attctccata tcattatgaa
    55381 gtataaccaa cttcgtcagg ctgaacaagc cgcgaaagct agtaaaagtc atatcgttgt
    55441 tattgaagag cgcaatcagg ccttgatgaa gaaagcaaac cgcttggttg aaaaacacca
    55501 gaatcttgaa aaagaaacca ttttggacat caataaatcc gtttccaaaa ttcgaacggt
    55561 tgaagaaaaa actgttatga aatccaccaa gattgaaagt tcacaagaat ttggtcaatt
    55621 tttgcgtcaa atgccggaat tgatggccaa taaacatatt gaaaggttac tgaatgaaat
    55681 ttttgaaaca gaaaataatt tagcacaatg gaagatttca tacaatcagc aggttgagta
    55741 tttcaatact ctgttgcatc agtttccgat tagcgtcatt gccaaaatca ttcgcctgaa
    55801 ggacttggaa ttttataagg aacattcgct gactgatttt acagacgaga tgttggggct
    55861 ataagataaa gaagggtcta tcaatgtatc ttaagtattt gataggcttt ttttgccatc
    55921 tttttgtaaa ttctctgtca ctactttatg aaaatcagac tatctgctgt aactagctta
    55981 attttcacgg agtataaaaa agtcagacta ggaatttgac actacttagt aataatgata
    56041 caatctccct atagtgataa aaactactat gtagtaacaa atagaaaaga ggaaaacata
    56101 tgtcaaactt tgcaaaactt caagaaactc gccgttcaat ctacgcactt ggtaaagact
    56161 tgccagtatc aaatgaagaa gtagtagcac ttgttgagaa agccatgaaa gaatctccat
    56221 cagctttcaa ctcacaatct agccgtgcag tggtactttt tggtgcggag tcagaagttt
    56281 tctggaacga aattgcctac agcgaattgg aaaaagtaac gcctgcagaa gcttttgaag
    56341 ctacaaaagg tcgcttagct ggttttgcgg ctggtgccgg tactatcttg ttcttcgaag
    56401 accaagacgt ggtgaaaggc ttacaagaaa gcttcccact ttacgctgaa aacttcccaa
    56461 tctggtttga acaagctcat ggtatcaact tgtacgcagt atggttggcc tttgctgaga
    56521 aaaatatcgg tatgaacgta caacactaca acccattggt tgatgcacaa gttgctgaaa
    56581 aatacggtat cccagctaac tggaaactcc gcgcccaagc accattcgga agcattgcag
    56641 ctccagcagc agacaaagac tatatggcgg attctgaccg tgtaaaggtt tttggtaact
    56701 aatactctgc gaaaattggg atcagacgtt gttgacttga tgtggtgagt gtaaagctcc
    56761 agtggagttt ttcagcctgt tcccttaaaa caagagagga gcagggttat gcctgcagct
    56821 tgttgacacg aactccattc gtttcatctc caagcttcaa ctatctccca gagctaacct
    56881 tttcaacttc ctgtatcagc aggaagtttt tttatctgtc tagcttaaaa tatgatacaa
    56941 taatagggta tttcaaagta caaaggagaa aaagatggta ttaccaaatt ttaaagaaaa
    57001 tctcgctaaa tatgctaaac tcttggtttc aacaggtatc aatgtccaac ctggtcacac
    57061 ggttcaattg acaattggtg tggaacaggc tgagttggca cgcttgattg tcaaagaagc
    57121 ttacgctcac ggtgctaagg aagtcttggt caactggttg gacgatgtga ttgcgcgcga
    57181 gcgtttggtc aatgtagatg tggaactctt ggaacaagtt cacccacaac gcatcactga
    57241 aatgaactac ctcttggagc ggaaggccag ccgtttggtt gtcttgtcag aagatccagg
    57301 ggcctacgac ggtgtggatc cagagaaatt gtctcgcaac gctcgtgcac tcagccaggc
    57361 tttgaaccca atgcggcaag catcccaagc taacaaaatc agctggacac ttggtgcagc
    57421 atctggtttg gaatgggcca aaaaagtttt cccaaatgca acgtctgacg aagaagcagt
    57481 agacttactt tgggaccaaa ttttcaagac ttgccgtatc tacgatgaag atccaatcaa
    57541 agcctgggaa gagcatgaag cacgtttggt agcaaaagct aaggttctta acgacgagca
    57601 gtttgtcaaa ttgcactaca cagcaccagg aaccgacctt gttcttggta tgccgaaaaa
    57661 ccacttatgg gaagcggctg gttcgatcaa tgcccaaggt gagaagttta ttgccaacat
    57721 gccaacagaa gaagtcttta cagctcctga ctatcgtgtg gccgatggtt atgtaacttc
    57781 tacaaaacct cttagctata acggcaatat tatcgaaggt attaaggtaa cctttaaaga
    57841 tggtgagatt gtagatgtga ctgctgaaaa aggcgacgaa gtcatgaaga aactggtctt
    57901 tgacaatgct ggtgcacgcg gtctaggtga agttgccctt gtaccagata agagcccaat
    57961 ctctcaatca ggtgtaactt tcttcaacac tctctttgat gaaaatgcct caaaccactt
    58021 ggcgattggt caagcctatg ccttctctat cgaaggtggt acagaaatga gccaagaaga
    58081 gctcaaagaa gctggtctca accgctcaga tgttcacgtg gacttcatga ttggttctaa
    58141 caagatgaac attgacggaa tccgtgaaga cggtactcgt gtgccaatct tccgtgacgg
    58201 tgagtgggct atctaaaaac aatgctataa gactgggaga ccagtcttat tttgtaaata
    58261 aggaggaaaa tagaatgtta tcaagtataa tcgctggagc cattattggc ttgattgcgg
    58321 gggctttgac ctcatcagat gaccgtcgtg gttgtcttgg aaatatcatc cttggacttg
    58381 ctggttcgtg gattggacaa ctgctcttcg gtgattgggg actacagttt gctggtatgg
    58441 ctgtagtgcc gtctgttcta ggggctattc tcttagtggc gatttttagc agttctagac
    58501 ggaattaaac acctgaaccg tgcttcaatt ggagcatggt tttctattat ctggatttgt
    58561 acgaattgcc gcattgttcg gatttttcag aaaatttcca tttttctgtt gacaagtgaa
    58621 aataagtagg gtatcattgt aaataattaa aatactgaaa ggaaaaagtc aaatgaaacc
    58681 tgtcactaca aaattctttg ctttgttgtt gcgacggtcg ggctagtgca ctaggtagca
    58741 ttagtcctgt ttggcttacc aagcgggcgc tgttgacaac atctcgcagg taatagtcct
    58801 cgaggtgttt tctttttctt agtatacttt gaaaactaag aaagagaacc taatattgac
    58861 gttagggtta tttagaaagg tattcttatg cgtgtgattg aatttttaga tacgacttta
    58921 cgagatggtg aacagacgcc aggtgtgaat ttttctatca aggaaaaagt gaccatcgcc
    58981 aagcagttgg aaaaatgggg gatttctgct attgaagcgg gttttcctgc ggccagtcct
    59041 gattcgtttg aagcggttcg tcagattgcg gcagcgatga ccaagacggc tgtgacaggt
    59101 ttggcacggt cagtaaaatc agacattgat gcttgttatg aggctctgaa agatgccaag
    59161 tatccgcaga ttcatgtttt cattgcgact agccccattc accgtgaatt taaacttcaa
    59221 aagaccaaag aagaaatttt ggcgcaaatc actgaacatg tcagctatgc acgtgagcgt
    59281 tttgaggtag tggagttctc accagaggat gcgacgcgga cggagttgga ttatttgcta
    59341 gaagttgtgc agacagcggt agatgcgggt gcgacctaca tcaacattcc agatacagtc
    59401 ggctttacga ctcctcagca ctacggagaa attttccgtt acttgacaac aaacgtcaaa
    59461 tcggaccgtg agattatttt cagtccacat tgccacaatg acctaggaat ggctgttgcc
    59521 aatacccttt cagctattaa aaacggtgcc ggtcgtgtcg agggaactat caacggtatc
    59581 ggtgagcgag caggaaatgc ggcccttgaa gaagtagctg ttgcccttga aattcggaaa
    59641 gatttctatg atgtgaccag tccgattgtt ctaaaggaaa cgctcaatac ttcggaatta
    59701 gtatctcgtt tttctgggat tgccattcca cgcaataagg cggttgtcgg tggcaatgcc
    59761 ttctctcacg agtcaggaat tcaccaagat ggtgtcttga aaaatccatc gacctatgaa
    59821 atcattacac ctgagttggt aggggtcaag aaaaattctc tgccactggg taaactatct
    59881 ggtcgccatg cctttgtaga aaagctgaaa gaactagacc tgtattttga agaaggagac
    59941 gttgctagct tgtttgctcg tttcaaaaac ctggcagaca agaaggaaaa aatcacggac
    60001 gctgatattc gagctctagt agctgggaca gaaatcagca atcgtgatgg cttccaattt
    60061 aaagatttgc gtttggatag tcagtctgac ggaagtattc aggctcaggt catcttcatc
    60121 aatcaggatg aggaagaagt ggttgtttcg gagtcaggca agggttctgt tgaggctgtc
    60181 tttaatgcta ttgaccaatt cttccaccaa gagattagct tggaacgcta ccatattgac
    60241 gccatcaccg atggaattga tgcccaggcg cgtgtgcttg tcgccgtaga aaacaaggca
    60301 acggaaacgg tctttaatgc ctctggtatt gactttgacg tggtgaaggc ttctgccatt
    60361 gcctatattc atgccaatgt catggtacag aaagaaaata gcggacagat tgataagaaa
    60421 ctatctgaga aagagttacc acacatctag gaggaaatat gacgaaaaaa attgtagcct
    60481 tggctggtga tggaatcggt cctgaaatca tggaggctgg cctagcagtc ctcgaggctg
    60541 tggctgggca agtaggattt gattatgaga ttgaggagag agcctttggt ggcgctggga
    60601 ttgatgcgag cgggcaccct ctacctaagg aaaccttaca agcctgccgt aaggcggatg
    60661 cgattttgtt agcagctatc ggtagccctc aatatgatga tgccgctgtc cgtccagaac
    60721 aaggtcttct tcaacttcgt aaggaattag gtctctttgc caatatccgt ccggtgaaaa
    60781 tttttgatag cctgaaagac tattctcctc tgaaggtcga ccggctggac ggtgtggatt
    60841 tggtcatggt aagggagctg acaggcggga tttattttgg agagcatatc cttggaactg
    60901 accaagccag cgatagcaat acctatcaag ctgaggaaat cgaacgggtt gtccgctctg
    60961 cctttgacct agcccaaaaa agacgaaaaa aagtcaccag tattgataag caaaatgtgc
    61021 tagcaacgtc aaaattatgg cgtaaggtgg cggatgaggt agcgaaggac tacccagatg
    61081 tggtcttgga gcaccagttg gtggatagcg cagccatgct tttgattacc aatcctagtc
    61141 ggtttgatgt cttggtgacg gaaaatctct ttggtgatat tttgtcggat gaggccagcg
    61201 tattaacagg aacgctaggg gttctgcctt ctgctagtca tgcggtggcg ggtcccagtc
    61261 tctacgaacc tatccatggt tcggctcctg atattgcagg tcagggcatt gccaatcctg
    61321 tcagtatgat tctatctgtt gccatgatgc tggaagaaag ttttgatttg gtccaagcag
    61381 gtcagaccat tcgccaggca gttgaagcag tctttgcaga gggcattttt accaaggatc
    61441 taggtggtag cgcatctacc aaagaaatga cagcggccat tattgcccaa attggaggtg
    61501 ggtaaatatg agtggcaaat ctatttttga taagctctgg gatcgccatg tgattacagg
    61561 gcaagaaggt cagccccagc tcctctatgt ggaccagcac tacattcatg aggtgaccag
    61621 tccccaggcc tttcaaggat tgagggatac agggcgatct gttcggcgcc cagacttgac
    61681 ctttgcgact tttgaccaca acgtgccaac cgttgatatt cacaatatcc gtgatgtcat
    61741 ttccaaggct cagatggatg ccttggctaa gaatatagaa gatttcggca ttccccacgc
    61801 agcccatggt tcggaacatc aggggattgt ccacatggtt ggaccagaaa caggcaggac
    61861 ccagccaggt aagttcattg tctgcggaga tagccataca gcgactcacg gagcctttgg
    61921 ggctattgcc tttgggattg gtacttctga agtggagcac gttctcgcta cccagaccat
    61981 ttggcaggtc aaacctaagc gactcctagt agagtttgtc ggtcaggcgc aaagaggtgt
    62041 ctatgccaag gactttatcc tggctctgat tgcccgctac ggcgtagatt gtggtttggg
    62101 tcatgtggtc gagttcgcag gacccgtcat cgaccgactc agcatggaag agcggatgac
    62161 catctgcaat atgtccattg agttcggctc caaaatgggc atcatggctc cagaccagac
    62221 cacctttgac tacctgcgag gtcgggagtg tgcaccagcg gacttcgatg cggcggtggc
    62281 agactggaag gagctctact cggatgcgga caccgtctat gacaagcacc tggtccttga
    62341 tgtgtcggac ttggcaccca tggtgacttg ggggaccact cctgctatgg gggttagctt
    62401 tgacgaaact ttcccatcca tccgtgacca caacgacgag cgggtctatg cttacatgga
    62461 cttggctcct gggcaaaagg ctgcggacat tcccgttggc tatgtcttcc taggctcctg
    62521 tactaatgct cgcctcagcg atttgcagct ggcagctagg attgttaagg ggcggaaaat
    62581 agcagagcag gttacggcta ttgtcgtacc aggcagtcgc cctgtcaagc gtgctgcaga
    62641 aaagttaggt ctagacaagg tccctgcggg tgtccactgc gcctctacaa gtaatcgaaa
    62701 ctttgaagac cgacagggct ttggggccaa gacccacttg tgtagtcctg ctatggctgc
    62761 cacagctgcg ctttatggac gttttgtcga caccagacag ctagacattc tcaaggcggg
    62821 agtctaatgc accaatttac cacatggaca gggacaaccg ttccactcat gaatgataat
    62881 attgataccg accagctcct gcccaagcag tttctcaagc tgattgacaa aaagggcttt
    62941 ggcaagtatt tgctatatgc ttggcggtac ttggatgata actacacgga caatcctgat
    63001 ttcattctca atcagcctga gtatcaaggc gctagtatcc tcatatctgg ggataacttc
    63061 ggagcaggtt cttctaggga acacgctgct tgggctctgg cagattatgg cttcaaggtc
    63121 atcattgcag gctcctttgg agatattcat tacaacaatg acttgaacaa tgggattctg
    63181 cccattatcc agcccaaaga agtcagggac caactagctc agctgggtcc tgaccaagaa
    63241 atcacggttg acttggcaga tcagttgata cggacgcctt ttggggaatg cccatttgac
    63301 atcgaacagg actggaaaca caagttgctc aatggcttgg atgatattgg tattacctta
    63361 cagtatcagg atttgattgc tgagtatgag gataatcgcc caagctactg gcaaaactaa
    63421 aaatattaga aaacaagcag atcgctctgc tatggaacaa aagaaaagag gaaattatta
    63481 tgacgaaaca aattcactgg gatggcggac tttcacaaga aggttttgac attatcaagg
    63541 gtgagggagg cgtgattgtc tgcccgacca aggttggtta catcatcatg actgctgaca
    63601 aggctggctt ggagcgtaag tttgatgcta aggagcgcaa gcggaataag ccaggcgttg
    63661 tcctttgtgg tagcatggag gagcttcgtg agttggcaca gttgactcct gagattgatg
    63721 ccttctacca aaaacattgg gatgaggaca ttttgttggg ttgtatcctg ccgtggaaac
    63781 cagaggctta tgctaagttg caggcttatg gcgatggtcg tgaagagttg atgaccgacg
    63841 ttcgtggtac ttcttgcttt gttatcaagt ttggtgtagc aggcgagcag attgctaagg
    63901 aaatgtggga aaaagaaggc cgtatggtct atgcgtcatc tgctaacccg tcaggtaagg
    63961 gaaatcgtgg caaggtagaa ggtatcggtg agcgtatcgc ttctaaagtg gacttgatta
    64021 tcgaggcgga tgattatgtg acatctatcc agccagacaa gaccattgaa acccgctatg
    64081 agcaaggggt tatggtgtct atggtagaca aagatggtaa actcattcca gaacaaagcg
    64141 cggatagccg ttctatcagc ccatgtccag ttgtcatccg taagggtctg gacattgata
    64201 aaatcatgat gcacttgtct gaccatttca actcttggga ctacagacaa ggggaatatt
    64261 actaagatat cgtcatttag tttatctttt attacgtcgt taactcacct tgcctaactc
    64321 cagttatgcc tgcggctcgt tgcctagtac taaaagtaaa ctaaaagact attattgaga
    64381 tcgatccgaa agggtcgttt tttcgtactc acataaaaaa ctgaccgaaa atttttcagt
    64441 cagtttctta tgtttattca attaccaaca tgccttcttt aacggcaaat gggtgttctg
    64501 ggtcaatgcg gtcgtagaac atgacaccgt tgaggtggtc gatttcgtgt tggaccacga
    64561 tggcattaaa gcctttgagt ttgatacggt gtttttctcc gtttttgtcc atgtaatcaa
    64621 cagtcacgcg cgcatggcgg actacgtagc cttggacttc gcggtcaacg gataggcagc
    64681 cctcgccccc ttccatagca gcttcttgga cagaatgtgc cacgatttta ggattgtaca
    64741 atacttcttg cagagagtag gcttgggcag gtggatttcc ttcctcgtcc tctgggtttg
    64801 ggactaagac agcaatgatg cgtttggaaa cgtcaatctg aggtgcagct agaccaactc
    64861 cgccacggag tttcattttt tcagccatga caggatcctg cgaatgtttg aggaactgca
    64921 tcattttttc gcctaaaata atttcctgat cagatagtgg aaatgcaact tcttcggcaa
    64981 cttggcgtag ggttggatgc ccttcacgga tgatgtcatc catgtcaatc aaatgggcag
    65041 cttttgtcaa tcgttcaata acagacatga ttctctcctt tgaatatagt taagataagt
    65101 atagcatgaa atgggagaga ataaaagtcg gacttttcta tccattcgct ttcaaatctg
    65161 ttatactagt aaaagacgaa cgttgaaagg gagaaaaatg aaaagaattt tagaaaaagc
    65221 cagtttgctg gccctatcca ccatgctggt ttcgacattt gctgtctcgc cagctattcc
    65281 acagatgatt gagtattttg cccagcaggg aattgcagcg gcccaggtgg aaaacttaat
    65341 tacggtgacc tcttttgcta ttatggttac tctattggca aacggtctag ttgtccgttt
    65401 cttgtctgaa cgaaacatta tcattgcagg gcttttgctt atggccatcg gcggagcttt
    65461 gcccatgttt ctaactgctt atcctctgat tttattggct cgcatcttgc ttggtttggg
    65521 gattggccta atcaatgcgc gtgctattaa tatcatcggt aattttttca ccggtcagga
    65581 gcgggtgcag atgatgggct tgcgtggttc agcagaggtt ttgggaagtg cagggcttac
    65641 cctacttgtc ggttggttga cccagtttgg ttggcaacct gccttcttgg tctacctttt
    65701 tgccttggtc atcttggctc tcttccttat tttcgtaccc aaggaggaat tgctgtctcg
    65761 catggaggta aaggttgagg agaacgcgcc caaggtcaaa ctggacaaga ccatgtggat
    65821 gatgggcatt tatctggcat tcttagcctt ctttgttatc aatgtcaata ccttcctgac
    65881 cattcgtatt cctcagattg ttttagccaa gggcatcggt actgctcagc aagccagtct
    65941 cattctaagt cttatgcagg tcatggggat tgtggcaggg acggtcttta gtagcttggt
    66001 tggtcgtttg aagggctggc ttttagctgt ttcctacgtg gtctttggac tcgcggttgt
    66061 cggcattgcc tttgcagata atctctgggt gctgggactc ggtggcatgg tgtcaggatt
    66121 tttctacagc atcattctaa ccattgtctt tagtcaggta acagaccgtg cacccaaggc
    66181 cttgctcaat gcggtcatga caatcgtcct tatgggctgc aatatcggcg gtgcgacatc
    66241 tgctatcttg ccgacttatc tggaaaaact caacccaacc ccaacaggtg cctttggtat
    66301 ttacgccatc ggctgtgcct tgataagcgc aggcttgatt tataaacaac tgaagaagtg
    66361 ggagtgagaa agaactcgac tggtctaaaa agagttcgtc aacaagtcct tatttccagt
    66421 tgttgagctg aaacagtcta tccccagact gtttcactcc cacccccgca cagctcaaac
    66481 tgtctgggag acagtttgag gttggagatg aagcgaactc tgttcgcatc agtcgtatag
    66541 gtcagatttg gagtgtaata cacgaacaaa tctgccaatc aaccactgcg ctgagatgtt
    66601 gacacgaact ctaagtagta gccgttgggc tttttgccca gcctcttttg gtataatatt
    66661 cttatgttag aaaataaccg cttaaatgaa ttaatactcc gttttccaga ggatgttcag
    66721 ttgattttta aggacatgat tccctcctat cagttgggct atgtggatta tgtttatcag
    66781 acagatagtt acgccaccca aaaccgtcgg attaaaaatt tagcaaaaaa tttctgaaga
    66841 atggattatt tttacatcat gcccctgccc caggatctgg cattggaaat tgctaaccaa
    66901 tggcgctatc aaccaccttt ggatgcctat acgattagtg caaaccccga gatttatgct
    66961 gtgatgattt caccagaggc gcgtggggac cgcttttttg cagtcatccg caatgctgcc
    67021 ctcatgggct atttctgtat tgaccaagat ggcgagacag ttgacattcg tttgggcatg
    67081 aaaccttctt tgacaggtca gggaaatggc agagctttct accaggctat tgaagactat
    67141 ttagtagagc atgttcagcc agctagtatc aaactgacag ttgctagctc tcatcaagtt
    67201 gcccaaaaac tctttcatgc tcttggtttt acagaaatag aaaagcaagc agaatatatc
    67261 aaaatggaaa agaaactcgt atgcgattag ataaattttt agtggattgc ggtgttggca
    67321 gtcggacaga agtcaagaaa ctcctgaaaa acaagcaagt cacagtcaac ggccaagtag
    67381 aaacatcacc caaacagcaa attaacgaaa aaaaagacca gattgcagta gcaggtcagg
    67441 tccttagtca tgaaaccttt gtttactatc tgttaaacaa gcctaaggga gttatttcag
    67501 ccaccgagga tgaccaccat cggactgtct tggacctgct agataataca gctcgccaga
    67561 aggaagtttt tcctgttgga cgcttggaca tcgataccca cggtctcttg cttttgacca
    67621 acaagggtca gctggctcat gccatgcttt ctccgaaaaa gcatgttgat aaactctacc
    67681 gagcacaggt tgacgggatc atgacccaag aggatgtcga acgatttgcg gcaggaattg
    67741 agctgaaaga ctttacctgt caaccagccc agttgaccat cctctctaca gatgaagcta
    67801 agcagacctc gctcgttgac atcaccatcc gagaagggaa atttcaccag atcaaacgca
    67861 tggtgcaggc ttgtggaaag acggtgacag acttgcaacg attgaccatg gggcctcttc
    67921 gcttagatcc aaaattagcg cttggagaat accgcaggtt gactgctgcc gaattaaagc
    67981 aattagaagt ctttggtgtg gaattataga taaatagaag agacttgctc ccgattggag
    68041 gtcttcttct ttttggggta agtaaaaaat gctaagggtg tacagttgtc gtacctgaaa
    68101 ttctagcccc tacttgaccg tacttggcat aacatattac aatatataca agaacctgtg
    68161 ttcacagttc atagcttcca ttggagtgtt tgaaggtgga gataaggaaa ggagattcct
    68221 gaccaattag gtggtaggcg aaaacagcca caggagcttt ttactcgtcg aagcaataaa
    68281 gtgaaactaa atggtgatag agtcaagtat tccttcaaaa aattgccttg atgagcaaaa
    68341 acgatatctt gcataagaac actttgttta tgaatatagg ttctaagaat tgtccaagag
    68401 aggttattat atgttgaaat atatccgtgc ccttcccctt gttatagtag cgggagcggt
    68461 tgtttacttt actattttta tctgtattga taatggtgat tggacctatg ttgtgactag
    68521 agcgctaggg cttaaggaag aacatgccaa taatgtttct ttgattgtaa ttggtgccca
    68581 atcacttttt ctaacttcgg attggtttta caagcggatt cgtgcctttc tcaatcgtcg
    68641 tttggaaaag gccaaagggc ttaatcggca gatttatctg ggctataagg cagggattga
    68701 gtttttgatg gcgttaccca aacgaactat tttattcaca ttctatctac ttttagtagt
    68761 tgctgaaagt gttggtctga ttgaaaaagc caaggactat gctgcggtaa tcatcttcat
    68821 aattgctgtt gaccgcgtga ccaaaatctg gccggaggaa aaggggagga tgattgagtt
    68881 tagccagaag attaagaaac ggattaagag tgtg
//
LOCUS       DBNFCK010000003        40671 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.003, whole genome shotgun sequence.
ACCESSION   DBNFCK010000003 DBNFCK010000000
VERSION     DBNFCK010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 40671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40671
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.003"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            55..846
                     /locus_tag="AC1PIW_000142"
     CDS             55..846
                     /locus_tag="AC1PIW_000142"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIP6605118.1"
                     /translation="MKLLTINVHAWLEERQDEKLDVLAQTIAEKAYDVIALQEVNQLI
                     TADLVTKDLKADNYGLILLEKLRTLGQFDYSYYWSNSHIGYDKYEEGIAFLTKLPVYE
                     VDQFYCSQNKSIDSILSRKIMGLTVFYQDQLIDLYSCHINLPGSEEEDQLDNIRTIVE
                     RTGNRRFKILMGDFNTDAFSDREAYEAITELGLYDSYNLAMEKDQGITVEKAIDGWAG
                     HSQEKRLDYIFLNQKKEVLSSLVIFNGENRPIISDHFGVEVEINV"
     gene            880..3048
                     /locus_tag="AC1PIW_000143"
     CDS             880..3048
                     /locus_tag="AC1PIW_000143"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HIP6605119.1"
                     /translation="MKKFLSFEFWQKFGKCLMVVIAVMPAAGLMVSIGNSIPLISPES
                     ELLIRIGNIIAQIGWGIIGNLHLLFALAIGGSWAKEKAGGAFSAGLAFILINLITGHF
                     FGVTTDMLADAAATVTTVFGTEIPVSGYFVNILGQPALNMGVFVGIIAGFVGATTYNK
                     YYNYRKLPDVLTFFNGKRFVPFVVIYRSVLVALGLAIFWPVVQTGINSFGKWIASSQD
                     TAPILAPFVYGTLERLLLPFGLHHMLTIPMNYTSLGGTYDILTGAQAGTQVFGQDPLW
                     LAWITDLINLKDAGDMAQYNDLLANITPARFKVGQMIGSSGILMGLTYAMYRNVDEDK
                     KHKYKGMFISSALAVFLTGVTEPIEFMFMFAAMPLYVVYAFVQGAAFAMADIVNLRMH
                     SFGNIEFLTRTPMAIKAGIGMDVINFIWVTALFAVGMYFIANFMIKKFNLATAGRNGN
                     YDTETTDVVSNSNVDTADANSQVVQIINLLGGRDNIADVDACMTRLRVSVKDVAQVGD
                     ENAWKQAGAMGLIIKDSGVQAVYGPKADVLKSDIQDLLESGVAIPRTEIVATEEVAPE
                     TQFKGVTETVYSVAEGQAIAITEVKDPVFSQKMMGDGYAVEPSSGNVYAPVSGIVTSV
                     FPTKHAVGILSDKGVEVLVHIGLDTVALNGAPFSTKVTDGQRVEAGDLLLVADLEAIR
                     SAGRETTIVVAFTNTAEIKSVSLENLGQVSQDSQVATVEL"
     gene            complement(3119..3745)
                     /locus_tag="AC1PIW_000144"
     CDS             complement(3119..3745)
                     /locus_tag="AC1PIW_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HIP6605120.1"
                     /translation="MIRHKLFTSLQEEEHWINSIQSEGYQLVKVTPWTATYHFEKCSR
                     TPHPVRLDFHEHIAKGEYSNYLSLFEDCGWQPIQGSRRCGIHYFQQTIETSSTEIFSD
                     TESKKAFYSRYQNYAYSYFGLFLTLFFIHYQVGLQNGYTLWNPKSWYLTPGLWERTGT
                     SFWFGFLFETPFALFRSGIIPLFFLAWSIYFLHIAEKGKKEAKKLEKY"
     gene            complement(3756..4067)
                     /locus_tag="AC1PIW_000145"
     CDS             complement(3756..4067)
                     /locus_tag="AC1PIW_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIP6605121.1"
                     /translation="MKRNKHLPLTETTYYILLALLEPAHGYHIMQKVEDMSDGDVKIA
                     AGTMYGAIENLLKLKWICSVPSSDKRRKVYQVTATGQEIIKLETQRIQKLHHIAKELG
                     F"
     gene            4228..4944
                     /locus_tag="AC1PIW_000146"
     CDS             4228..4944
                     /locus_tag="AC1PIW_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIP6605122.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPETN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFVEGRYEGFGPNGSMIIVDTLTSN
                     VNRTAANVRSAFGKNGGNMGASGSVSFMFDKKGVVVFAGDDADAIFELLLEADVEVDD
                     VEAEDGTITVYTAPTDLHKAIVALKESGIQEFNVTELEMIPQSEVSLEGDDLATFEKL
                     YDALEDDEDVQKIYTNVDGF"
     gene            5308..6156
                     /locus_tag="AC1PIW_000147"
     CDS             5308..6156
                     /locus_tag="AC1PIW_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM hydrolase/SAM-dependent halogenase family
                     protein"
                     /protein_id="HIP6605123.1"
                     /translation="MSNNLLVLQSDFGLVDGAVSAMIGVALQESRDLVVHHLTHDITP
                     YNIFEGSYRLFQTVEYWPEGTTFVSVVDPGVGSKRKSVVALTEQNHYIVTPDNGTLSF
                     IKKYVGIKAVREISEVANRRANTEHSYTFHGRDVYAYTGAKLASGHISFEEVGPELSV
                     DKIVEIPTVPTEVGSDYVKGAIDILDVRFGSLWTSITREEFYTLQPQFEDRFEVTIYN
                     NDMLVYQNQVTYGKSFADVRIGQPLIYINSLYRVGVAINQGSFAKAYNVGVGQNWHIE
                     IKRISN"
     gene            6177..6722
                     /locus_tag="AC1PIW_000148"
     CDS             6177..6722
                     /locus_tag="AC1PIW_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HIP6605124.1"
                     /translation="MKNNSIKTVVATGIGAALFVVIGHLINIPTFVPNTSIQLQYAVQ
                     SLLAVVFGPVVGFLVGFIGHTLKDSLTYGPWWSWILASGVFGLVVGLTKKRLRIQEGI
                     FEGKDILFFNLVQIAANVLAWGVIAPVLDILIYSEAANKVFAQGLVAGIANSITIAIA
                     GTLLLVVYAKSQTKTGSLSKD"
     gene            6830..7750
                     /locus_tag="AC1PIW_000149"
     CDS             6830..7750
                     /locus_tag="AC1PIW_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605125.1"
                     /translation="MVWTEEKLGPIIHSILNPDEEVLSLYQSKTTKSIYGCILWNNYY
                     YKVFRVSNHPSQSTYKQPTFYDFHGEFYMKGAIRTYLYHTNSWYELSKQGYYLLLFFQ
                     KLWDEKQEVEASWQNGRMQIRLVVEEEGWQVHYRFPDNQAREVERLLGSGMLVAIRSS
                     RNFIKIRTSRFVAPYIALIKQRQLYRKKPSKAMLQWEKYQSQLIEIQRTYRLVEESAP
                     QTFFGHWLNKVQLYLCSAIASYWEEVIKGVEWQEAREIKEQRKAEKPHNPIEDKWKEN
                     IARRHKRKMDQLIGHDTLEKLRQLKTELDD"
     gene            7963..8946
                     /locus_tag="AC1PIW_000150"
     CDS             7963..8946
                     /locus_tag="AC1PIW_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HIP6605126.1"
                     /translation="MSKDIRVLLYYKYVPIENAKEYAAEHLAFCKSIGLKGRILIADE
                     GINGTVSGDYETTQKYMDYVHANPLFSDLWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDIDPLVTTGAYLSPKEFKEALLDEDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVRGELWDGKMYVFDERIAVDVNHVNPVVVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEHKYVRGCSAECRAHERNRYISENGLTRQEWAERLEAIGETLTPANA"
     gene            9273..9515
                     /locus_tag="AC1PIW_000151"
     CDS             9273..9515
                     /locus_tag="AC1PIW_000151"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIP6605127.1"
                     /translation="MPKGGNLILMQADSFAQRVPFQVVASGNEVLISLNVASREEVDR
                     IIERVEANGGQITGCPTDARGFYGASFTDLDGIILM"
     gene            9633..11306
                     /locus_tag="AC1PIW_000152"
     CDS             9633..11306
                     /locus_tag="AC1PIW_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6605128.1"
                     /translation="MKKIIEFKDFTFKYQAQQEPTLKDIQLTIYQGEKVLIIGPSGSG
                     KSTIGQCLNGIIPNIYKGEHSGSLLLDGQEAFNLSVYDKSLLVSTVLQDTDGQFIGLS
                     VAEDLAFALENDMESQVVMKQKVGDWAERLSLTDLLAHRPQDLSGGQKQRVSLGGVLI
                     DESPILLFDEPLANLDPKSGQDTIDLIDRLHKQEGTTTIIIEHRLEDVLYRQVDRVVL
                     INDGRILFNGGPDDLLRTSLLQENGIREPLYLSTLRALGYPIAQAERLSSVWEIDIEA
                     LNIASLPTLHFENGEKEALLEVKHLYFSYPNKQVLTDIQLTIHKGERLAIVGKNGAGK
                     STLAKVLCGFIQGEGEILWKGQSIKEDSVKERAERIGYVLQNPNQMISQTMIFDEVAL
                     GLRMRGVTEEEIEHRVHHILKVCGLYPFRNWPISALSYGQKKRVTIASILVLNPEIIL
                     LDEPTAGQDQRHYREMMEFLDQLNAQGHTIVMITHDMQLMLDYSDRAVVVVDGQIIED
                     ASPAEILSDDTVIERANLKETSIFHLAERVGVNPLELTTFYMQEERRGR"
     gene            11303..12136
                     /locus_tag="AC1PIW_000153"
     CDS             11303..12136
                     /locus_tag="AC1PIW_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T family protein"
                     /protein_id="HIP6605129.1"
                     /translation="MSQQKLIGYHPGTGLIHSLSAVSKLLFFLIVSILAMITYDTRLI
                     LFIAVFSLALFKMSGIRYKEISLVLILTIIFAAMNALMVHLFAPRYGVELYGADTPLL
                     SGLGVYSLTSQQAFYLVNLLLKYFCTVPLAIIFLMTTHPSQFASSLNQIGVSYKVAYA
                     VSLTMRYIPDIQEEFYTIRMSQEARGLELSKKGKLMDRIKGNLSLVIPLIFSSLERID
                     TISTAMELRRFGKNKKRTWYTQQPLQRIDYAVLLFILALVVVTIYLFFVNQGRFYNPW
                     R"
     gene            12296..12499
                     /locus_tag="AC1PIW_000154"
     CDS             12296..12499
                     /locus_tag="AC1PIW_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIP6605130.1"
                     /translation="MVQGTVKWFNAEKGFGFIAQENGPDVFAHFSEIQSNGFKSLEDG
                     QKVTFEVEQGQRGLQATNITKIG"
     gene            complement(12541..13254)
                     /gene="rsmG"
                     /locus_tag="AC1PIW_000155"
     CDS             complement(12541..13254)
                     /gene="rsmG"
                     /locus_tag="AC1PIW_000155"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIP6605131.1"
                     /translation="MKPEVFYKTLADQGIQLTDQQKHQFHRYFQLLVEWNEKINLTAI
                     TEESEVYLKHFYDSIAPLLQGHIQNEPLRLLDIGAGAGFPSLPMKIIFPQLDVTIIDS
                     LNKRINFLHLLAEELELEGVHFYHGRAEDFAQDKNFRAQFDLVTARAVARMQILSELT
                     IPYLKLHGKLIALKASSAEDELTQAKNALNLLFAKVIENHDYTLPNGDPRTLTIVEKK
                     KETPNKFPRKAGMPNKRPL"
     gene            complement(13313..15487)
                     /gene="pbp1a"
                     /locus_tag="AC1PIW_000156"
     CDS             complement(13313..15487)
                     /gene="pbp1a"
                     /locus_tag="AC1PIW_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP1A"
                     /protein_id="HIP6605132.1"
                     /translation="MKTTTIKKALLITANILLAGAIIACLAGAALVVYYIQSAPELTE
                     ESLTATVSSKIYDKNGNLIADLGAEKRSSAKTEEIPTDLVNAIVAIEDQRFFNHRGVD
                     VIRIAGSLINNLSGGRLQGGSTLDQQFIKLTYFSTSVEDQNLKRKIQEAWLATQLERR
                     NTKQEILTYYINKVYMSNGNYGMKTAALAFYGKELKDLTLPQLALLAGMPQAPNQYDP
                     YTNPEDAKARRDLVLAEMLEEKYIDNTQYEQAVLTPVTDGLQPLSNAAAYPAYMDNYL
                     KQVVEEVEAKTGYNLLTTGMDVYTNVDPAAQQQLWNIYNTDMYVSYPDDLLQVASTVV
                     DVSNGKVVAQLGGRKQETNVSFGTNQAVETNRDFGSTMKPITDYAPAFENGIYTSTAD
                     LILDGPYNYPGTPTPVNNWDKQYYGNISVKTAIQYSRNVTAVKALEATGLENALKFLN
                     SVGINYPDIHYSNAISSNTSDTSSKYGASSEKMAAAYAAFANGGTYYAPQYVNKIVFS
                     DGTVTEYAPKGTKVMSAETAYMMTDMMKAVMSYGYGLNASVSGVPMAGKTGTSNYTDS
                     ETDTILASIPEANYSYMVVPDENFVGYSSQYAMAVWTGYTNRMTPILDNSMRIATDVY
                     HNMMLFMHSDYTATDWEMPSGLVRYGSNYYLRGSRSLSNAYNTYTSSSTSSSSSTETS
                     ETTEATTTSSETSSGTATETATSSTETATTGNNPATGQTDGQ"
     gene            complement(15468..16085)
                     /gene="recU"
                     /locus_tag="AC1PIW_000157"
     CDS             complement(15468..16085)
                     /gene="recU"
                     /locus_tag="AC1PIW_000157"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HIP6605133.1"
                     /translation="MVNYPHKVSKKINRTSPISSQRVNFANRGMSFEAAINDSNQYYL
                     AHDIAVIHKKPTPVQIVKVDYPKRSRAKIVEAYFRQASTTDYSGVFKRHYIDFEAKET
                     RQKASMPMKNFHAHQIEHMKQVVKQGGICFVLLHFSTLKETYLLPATHLIEFYQVDMG
                     SKSMPLTFIRQYGFEIQMGRFPSIPYLEIVEKNLLGGESFENYNN"
     gene            16151..16690
                     /locus_tag="AC1PIW_000158"
     CDS             16151..16690
                     /locus_tag="AC1PIW_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HIP6605134.1"
                     /translation="MDTKSLLVTGYRHTDLGIFSEKDPRLHIIKSAIRRNFIRFLEEG
                     VSWFILTGQLGFEYWSLEVLEDLRAEGYQLSIATIFPFENHGEQWNEANQAKLARFKQ
                     VDFVKYAYPRYENPGQFRDYNQFLLDNTTGCYLFYDSENETNLKYLYHMVLKKEGYNR
                     KTLTFEELNEEAENFSNSE"
     gene            16761..17096
                     /gene="gpsB"
                     /locus_tag="AC1PIW_000159"
     CDS             16761..17096
                     /gene="gpsB"
                     /locus_tag="AC1PIW_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HIP6605135.1"
                     /translation="MASIKFTTKDIFEQDFKIGFRGYDQDEVNDFLDDIMKDYDAYEA
                     IIKELKGEIARLKAQAANSPKTTLPTEESNDVLRTERPSSATNFDILRRLNRLEKEVF
                     GKQIVQDQE"
     gene            17235..17599
                     /gene="rnpB"
                     /locus_tag="AC1PIW_000160"
     ncRNA           17235..17599
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="AC1PIW_000160"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            17653..18819
                     /locus_tag="AC1PIW_000161"
     CDS             17653..18819
                     /locus_tag="AC1PIW_000161"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing class I SAM-dependent
                     RNA methyltransferase"
                     /protein_id="HIP6605136.1"
                     /translation="MKKQFELIATAAAGLEAVVGREIRNLGYECQVENGRVRFQGDVK
                     SIIETNIWLRSADRIKIIVGQFPAKTFEELFQGVFNLDWENYLPLGCKFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHFSRPEGVPLQEMGAEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRVEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAAMLAKN
                     IAPGLKRSFAFEEWPWVEDQLVVALRKEAQASIKTDLVLDITGSDIDARMIEIAKKNA
                     FAAGVEQDIVFKQMRVQDLRTDKINGVIISNPPYGERLLDDEAIVTLYREMGETFEPL
                     KTWSKFILTSDELFETRFGQQADKKRKLYNGTLKVDLYQFFGQRVKRQVQEVQG"
     gene            18829..20283
                     /locus_tag="AC1PIW_000162"
     CDS             18829..20283
                     /locus_tag="AC1PIW_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HIP6605137.1"
                     /translation="MGEKNSHHLPLDEEKVLDFEVAKDLTIEEAVKKHKEIEAGVTED
                     DGLLDRYIKQHRAEIESQKFETKINHLPLVEVADEEKNQGHESAEEVEANESSLTEVS
                     EEIAPIVEELSVTPMETLEETVIASTVAMEGLSSVADDSSLELEEDETEDLDHSEGAD
                     RDQKKKFYFWSAVGLSMIGVMATALVWMNSVNKSNTATSSSSTSTSQTSSTASSSTDA
                     NVTAFEQLYNSFFTDSSLTKLKNSEFGKLAELKVLLEKLDKNSDSYTKAKEQYDHLEK
                     AIAAIQAINGQFDKEVVVNGEIDTTATVKSGESLSATTTGISAVDSLLASVVNFGRSQ
                     QEVASATVASEAAVTRNQGADETVSTGVPATTEVASTTVSGSTTDFGIAVPAGVVLQR
                     DRSRVPYNQAMIDDVNNEAWNFNPGILENIVTISQQRGYITGNQYILEKVNIINGNGY
                     YNMFKPDGTYLFSINCKTGYFVGNGAGHSDALDY"
     gene            complement(20666..21148)
                     /locus_tag="AC1PIW_000163"
     CDS             complement(20666..21148)
                     /locus_tag="AC1PIW_000163"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002885585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HIP6605138.1"
                     /translation="MKKEVTVESFELDHTIVKAPYIRLISEEVGPKGDIITNFDIRLI
                     QPNENAMDTAGLHTIEHLLAKLIRQRIDGLIDCSPFGCRTGFHMIMWGKQDSEKIAQV
                     IKSSLEEIAEGITWEDVPGTTIESCGNYKDHSLHSAKEWAKLILSQGISTDAFERKPI
                     "
     gene            21278..22891
                     /locus_tag="AC1PIW_000164"
     CDS             21278..22891
                     /locus_tag="AC1PIW_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HIP6605139.1"
                     /translation="MNIITVVVTLVSALIGLVLGYFAISLKMKSAKETAELTLLNAEQ
                     EASNVRGRAEEQAEVILKTAERDRQTLKKELLLEAKEEARKYREEIAEEFKSERQELK
                     QIESRLTERATSLDRKDDNLTNKEKTLEQKEQSLTDKAKHIDEREQEVVKLEEQKALE
                     LERVAALSQEEAKEIILTDTRDKLTHEIATRIKEAEREVKERSDKMAKDLLAQAMQRI
                     SGEYVAEQTITSVHLPDDAMKGRIIGREGRNIRTFESLTGIDVIIDDTPEVVVLSGFD
                     PIRREIARMTMETLLQDGRIHPARIEELVEKHRVEMDNRIREYGEAAAYEVGAPNLHP
                     DLIKIMGRLHFRTSYGQNVLRHSIEVAKLAGVLAAELGENVNLARRAGFLHDVGKAID
                     REVDGSHVEIGTELAKKYKENPIVINAIASHHGDVEATSTIAVIVAAADALSAARPGS
                     RRESMEAYIKRLQDLEEIANSFEGIKQSYAIQAGREIRIIVHPNKVTDDQITILAHDV
                     REKIENNLDYPGNIKITVIRETRATDVAK"
     gene            23046..23672
                     /gene="gmk"
                     /locus_tag="AC1PIW_000165"
     CDS             23046..23672
                     /gene="gmk"
                     /locus_tag="AC1PIW_000165"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIP6605140.1"
                     /translation="MKERGLLIVFSGPSGVGKGTVRKEIFESSDNKFEYSVSMTTRPQ
                     RPGEVDGVDYFFRSREEFEDLIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDGVFIFLTPPDLEELQDRLIGRGTDSEEVIAQRIERAKEEIALM
                     REYDYAVVNDEVPLAAERVKRIIEAEHFRVDRVIGRYDEMIREQIVER"
     gene            23696..24010
                     /gene="rpoZ"
                     /locus_tag="AC1PIW_000166"
     CDS             23696..24010
                     /gene="rpoZ"
                     /locus_tag="AC1PIW_000166"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIP6605141.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAKPTQEFTSV
                     KSTLRALEEIESGNVVIHPDPEAKREAVRRRAEEAKRLAEEEERKIKAQIAKEKEEGE
                     KI"
     gene            24203..26593
                     /locus_tag="AC1PIW_000167"
     CDS             24203..26593
                     /locus_tag="AC1PIW_000167"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HIP6605142.1"
                     /translation="MLAQIIVDVPLMQTDQAYSYRVPAELEEGLKVGVRVHVPFGKGN
                     RLIQGIVVDLIDEDDENSLQDLKELAEVLDYSPVLNQEQFWLADQMRKSVFSYKITLL
                     KSMLPSLLNSTYDKLIRPGIGLEMAEQISLFGDKEQIRFSDLDVTEQGKIMRLAQSGK
                     ILVDYVAKDKKQIKTEKWYRVCLEKLREADISNRAKKRQQLREFLLEQSEDQLLSNLK
                     SDYSADTLRYFQEKGYIEVWEEEVSRTQGVFDKVEKTQALDLNPEQAIAVREIVASIG
                     QESQTFLLQGVTGSGKTEVYLQVIDRVLKMGKTAIMLVPEISLTPQMTNRFISRFGQQ
                     VAILHSGLSDGEKYDEWRKIEAGNAQVVVGARSAIFAPLTNLGVIIIDEEHEATYKQD
                     SNPRYHARDVAKLRADYNRATLVLGSATPSLETRARASRGVYGRLTLNQRANPLARIP
                     EVEVVDFRDYIGQQEASNFTPVLIEKIREKLARKEQVVLMLNRRGYSSFVMCRDCGSV
                     DQCPNCDISLTLHMDTKTMNCHYCGFQKGIPQTCPNCQSRSIRYYGTGTQKAYDELQE
                     LLPEARILRMDVDTTKKKGAHEDLLERFGRGEADILLGTQMIAKGLDFPNVTLVGVLN
                     ADTALNLPDFRSSERTFQLLTQVAGRAGRADKEGEVLIQTYNPNHYAIAFAKEQDYEG
                     FYRYEMDIRKNLGYPPYYFTVGLTFSHKSEEFVVKKAYETVAFLRQHLTDAIQILGPT
                     PKPIARTHNLYHYQIILKYRHEDRLEEVLNQILDWTQERENQDLRLIIDNEPQNMM"
     gene            26725..27663
                     /gene="fmt"
                     /locus_tag="AC1PIW_000168"
     CDS             26725..27663
                     /gene="fmt"
                     /locus_tag="AC1PIW_000168"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIP6605143.1"
                     /translation="MTKLIFMGTPAFSATVLRGLLADGNYNILAVVTQPDRAVGRKKV
                     IQMTPVKEVALEYNLPVYQPEKLSGSQEMDELMNLGADGIVTAAFGQFLPTKLLNSVD
                     FAVNVHASLLPKYRGGAPIHYALINGDERAGVTIMEMVKEMDAGDMISSDSIAIEESD
                     NVGTLFEKLAVVGRDLLLQTLPAYIAGDLKPVAQNPEQVTFSPNIQPEEEVLDWNKTA
                     RQLFNQIRGMYPWPVAHTYWQGERFKIQEAVEAEGEGSVGRVIARSKKELIIATGQGA
                     LSLKTVQPAGKPKMTIADFLNGAGRDIAVGDQFGDQ"
     gene            27650..28963
                     /gene="rsmB"
                     /locus_tag="AC1PIW_000169"
     CDS             27650..28963
                     /gene="rsmB"
                     /locus_tag="AC1PIW_000169"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HIP6605144.1"
                     /translation="MVINKHETARSLALSVLEQVFDQGAYSNIALNKALESSRLSAQD
                     KGLATELVYGTVSRKITLEWYLAHFIEDREKLDTWVYYLLMLSLYQLVYLDKLPTHAV
                     VNEAVNIAKRKPGTDKFVNAILRKLSQDSLPKPADIKRKNKRLSVQYSVPVWLVQTLI
                     AEYGDERAEKIFQSLHDRNKASVRVTDGQQVERLAEELGAQHSQLSPVGLVKPHGHFA
                     GTDYFKEGLITIQDETSQLVAPTLDIQGDEIILDACAAPGGKTCHMASYLTDGKVYAL
                     DLYDHKLNLIEENARRLGLADKIETKGLDASRVHEEFGPDTFDKILVDAPCSGIGLIR
                     RKPDIRYNKASMDFDSLKTIQLQILDSVCQSLKIGGIITYSTCTIIAKENQEVIQEFL
                     QNHPNFEQVTLEHPQTDIMVDGCLLITPEQYKTDGFFIGQLRRKS"
     gene            29001..29738
                     /locus_tag="AC1PIW_000170"
     CDS             29001..29738
                     /locus_tag="AC1PIW_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HIP6605145.1"
                     /translation="MEIALLTDVGQKRSNNQDYINRYKNRAGIDLVVLADGMGGHRAG
                     HIASEMAATDLGAAWVDTQLVTLNDVREWMVAIIDAENQKIHELGQTEEYKGMGTTLE
                     AVVVIDNQMIYAHVGDSRVGLIRDGEYTRLTNDHSLVGALVRAGQLTEEEAQRHPQKN
                     IVTQSIGQAEPIEPDIALKTLEVGDYVLINSDGLTNMIPTEDIRDIVLSDVSIESKVE
                     TLVRFANNAGGLDNITVGLLHITEEAR"
     gene            29738..31267
                     /gene="pknB"
                     /locus_tag="AC1PIW_000171"
                     /pseudo
     CDS             29738..31267
                     /gene="pknB"
                     /locus_tag="AC1PIW_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893497.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
     gene            31244..31732
                     /locus_tag="AC1PIW_000172"
     CDS             31244..31732
                     /locus_tag="AC1PIW_000172"
                     /inference="COORDINATES: protein motif:HMM:NF015734.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HIP6605146.1"
                     /translation="MAPQVVPNVVGKSQENATQILQTAGFSIGTITQEYSSSVTAGQV
                     ISTDPVANTELAKGSIINLVISKGKELIMPDLTSGNYTYSQARSQLQALGVNAESIEK
                     QEDRSYYSTTSDIVIGQYPAAGATIDGTVTLYVSVASTRTSSDSSAGSSTSTSTSTGS
                     GQ"
     gene            32153..32848
                     /gene="liaF"
                     /locus_tag="AC1PIW_000173"
     CDS             32153..32848
                     /gene="liaF"
                     /locus_tag="AC1PIW_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HIP6605147.1"
                     /translation="MRKVQFFLLIESMIFTMAAFDVVANEASRTLLLFSALLLVIWYF
                     LGRKVNSVLLVSSLSLLFLVFVLNPFFIIGIMLLVVYMLINFFSRYEKRNQYTHIVFS
                     DYSLQVQKEKNRWFGNHDHSQDRFGFEDINIVRLFGNDVVDLDKTVLVGRDNIVVIRK
                     TFGRTKIIVPIDVEVALSATSIYGRVSFLGESYWDLRNESIAVNSPDYQESHKRVKVV
                     TNNILGDVEVVRV"
     gene            32845..33861
                     /locus_tag="AC1PIW_000174"
     CDS             32845..33861
                     /locus_tag="AC1PIW_000174"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIP6605148.1"
                     /translation="MRKRTYLLLAWFACLIVFVVIASTLPLLNYSLLDVTLWISTEQL
                     VFTLILLVIVLTLFLAVMVQTVSLASTQVMRTKLQAILKNKSIRTDTESDQLLLQLSD
                     KVRNLTRQVQLVDNQDLVKKEEIVEGERKRIARDLHDTVSQELFATSMILSGLSSNLS
                     TLPQETLQEQLMLVKDMIESAQRDLRILLLHLRPSELESKTLVEGFELILREVSDKSN
                     IIVHFQHEVEELPKLIEEHLFRIAQEIISNTLRHAKAKHLDVYLLQKETELQLKMTDD
                     GIGFQQGADDELSYGLQNIRERVEDMAGTIKIRTAPNKGVAIDIRIPLLKGKEDEYDS
                     SDAG"
     gene            33833..34474
                     /locus_tag="AC1PIW_000175"
     CDS             33833..34474
                     /locus_tag="AC1PIW_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIP6605149.1"
                     /translation="MNTIRVMLVDDHEMVRLGLKSYLNLQPDVEVVAEASDGEEGLAK
                     ALEVKPDVVVMDLVMPKMTGVEATLALLKEWPQAQIVILTSYLDNEKIYPVLEAGARG
                     YMLKTSSADEILAAIRKVALGEYAIETEVEKKVEHHKRHPDLHDDLTAREREILTLLA
                     KGYDNQRIADESFISLKTVKTHVSNILSKLAVSDRTQAVVYAFQHGLVAQEDQ"
     gene            34524..35924
                     /locus_tag="AC1PIW_000176"
     CDS             34524..35924
                     /locus_tag="AC1PIW_000176"
                     /EC_number="3.1.3.-"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HIP6605150.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRVKNTGYIPESIQQVFKSLKE
                     KGILTGIASGRTPYGLVPEIKALKPDFFAMINGSYVEDAKGQVVYHQPMPQNLVESVL
                     NWAKEIGIEYGMLSSQKGTLSARTDRISQVIDLIYEGLETKPTFYKENDIYQLLTFEK
                     DGHEVELPEELQAELRSVRWDAISSDIVLKGSSKATGVAKVVEKLGLKPENVLVFGDG
                     LNDIELFDYAGISIAMGHSHPELQKHADYITKKVEEDGIFDALEKLGMVEKEKYFPQL
                     DLENVTGPVAHIKTNHGKLTVKLFPEIAPKTVANFVALSKDGYYDGIIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGTAFEDEFSMEAFNLRGALSMANAGPNTNGSQFFIVQNQNFP
                     YNAKELERGGWPKEVAEAYVKNGGTPYLDQRHTVFGHLVDEESFVVLDAIAAVATDSA
                     DCPHEDVVIETIEIED"
     gene            35926..36297
                     /locus_tag="AC1PIW_000177"
     CDS             35926..36297
                     /locus_tag="AC1PIW_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HIP6605151.1"
                     /translation="MKIGDKLKGTVTGIQPYGAFVQLENGTTGLIHISEIKTGFVDNI
                     YDHLKLGQEVLVQVVDFDEYTEKASLSIRTLEEEQQKIPCHHRFTNERNKIGFSPLAK
                     QLPKWIEEAKGYLKEHKEKKV"
     gene            complement(36323..37249)
                     /gene="cysK"
                     /locus_tag="AC1PIW_000178"
     CDS             complement(36323..37249)
                     /gene="cysK"
                     /locus_tag="AC1PIW_000178"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HIP6605152.1"
                     /translation="MAIYQNITQLVGKTPVIKLNNIVPEGAAEVYVKLEAFNPGSSVK
                     DRIALAMIEDAEKAGTIKPGDTIVEPTSGNTGIGLAWVGAAKGYNVIIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAKEIAEEKNGWVPFQFANPSNPKVHEDTTGQE
                     ILEDFGTTGLDAFVSGVGTGGTVSGVSHVLKTANPDIAIYAVEADESAVLSGEAPGPH
                     KIQGISAGFIPDTLDTSAYDGIIRVKSDDALATGRAIGGKEGFLVGISSGAAIHAAIE
                     VAKELGTGKKVLAILPDNGERYLSTALYEFND"
     gene            complement(37339..37971)
                     /locus_tag="AC1PIW_000179"
     CDS             complement(37339..37971)
                     /locus_tag="AC1PIW_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HIP6605153.1"
                     /translation="MKEFKTIREDGIVEEEIKKSRFICFMKRVTSEEEARDFINKIKK
                     EHYKATHNCSAFVLGENMEIKRSSDDGEPSGTAGVPMLTVLENHELTNVATVVTRYFG
                     GIKLGAGGLIRAYAGAVANAVKEIGVVEVKEQEGISLSMSYAQYQEFANWRAEQGLEE
                     FDTQFTTEVSTMIFVDKEFLEQTLASLTDFYHGKVSTEKANSRIVEVPVY"
     gene            38028..39320
                     /locus_tag="AC1PIW_000180"
     CDS             38028..39320
                     /locus_tag="AC1PIW_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIP6605154.1"
                     /translation="MKELENYYGRLFTKYQLTAKEREIAEKVPSITKKNNCFRCGTTF
                     EEENKLPNDAYYCRACLLLGRVRSDEKLYHFPQKDFPITKCLKWKGQLTDWQQRISDG
                     LVANVENNRATLVHAVTGAGKTEMIYHTVASVIDKGGAVCLASPRIDVCIELYKRLQN
                     DFSVPISLLHGESEPYFRTPLVVATTHQLLKFYQAFDLVLIDEVDAFPYADNPMLYRA
                     ADNAVKEAGVQVFLTATSTDELDKKVRTGKLSRLSLPRRFHGNPLVVPQKVWFSKFDD
                     TLKKNRLVPKLKKAIEEQRKSCFPLLIFVPEISKGQEFTKIMKKTFPEETIGFVSSQT
                     ENRLEIVEGFRKREITVLISTTILERGVTFPCVDVFVVQANHYLYTVSSLVQIAGRVG
                     RSMERPTGLLQFYHEGSTGAIEKAVAEIKQMNKEAGYV"
     gene            39313..39978
                     /locus_tag="AC1PIW_000181"
     CDS             39313..39978
                     /locus_tag="AC1PIW_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HIP6605155.1"
                     /translation="MSNCLLCGQAMKNKTRFSDLIFFSKEKSGICEECFSTFEEIAEQ
                     HCPHCYKNGESESCKDCQYWQNQGKPVVHTALYQYNQAMAHYFSRYKFQGDYVLRNIF
                     AKKLRIALSQFPDYTIVPIPISQKRLSERGFNQVEGLLDATNIPYQSLLGKYESQKQS
                     SKNRAERLEAKQMFYLLDEKEVPEKILLFDDIYTTGATIQLAVELFMKIGRKEIKTFS
                     LTR"
     gene            40055..40597
                     /gene="hpf"
                     /locus_tag="AC1PIW_000182"
     CDS             40055..40597
                     /gene="hpf"
                     /locus_tag="AC1PIW_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation-promoting factor, HPF/YfiA
                     family"
                     /protein_id="HIP6605156.1"
                     /translation="MIKFSIRGENLEVTEALRTYVEEKVAKIEKYFNEEQELNAKVNL
                     KVYRDKRAKVEVTIPVGAVTLRAEDISQEMYGSIDLVVDKIERQIRRNKTKIERKHRQ
                     KVATGQVFTDELVEQTGEEVKVVRTKQVDLKPMDMEEAVLQLELLGHDFFIYTDANDG
                     TTNVLYRREDGDLGLLEVRQ"
BASE COUNT        12333 a         7385 c         9170 g        11783 t
ORIGIN      
        1 tatactattc ctataaaaga aaacggttac gcattttcag agagaggggg agcaatgaaa
       61 ttactaacaa ttaatgtcca cgcctggtta gaagaaaggc aggatgaaaa actagatgtt
      121 ttagcccaaa caatagctga aaaggcttat gatgtcatcg ctcttcaaga ggtcaatcag
      181 ctcataactg ctgatttggt aacgaaagac ttgaaggcgg ataattatgg cttgatttta
      241 ttagaaaaat taagaacctt gggacaattt gactattcat attattggag caactcacat
      301 atcggctacg ataagtacga agaagggatt gcctttctta ccaagttgcc agtttatgaa
      361 gtggatcaat tttattgtag tcagaataaa tctattgatt ccatcctatc acgaaaaatt
      421 atgggattga cggtcttcta tcaagaccag ttgatcgacc tgtattcttg tcacatcaat
      481 cttccaggga gtgaagagga agaccagctt gataacattc gtacgattgt ggaacggact
      541 ggcaatagac gttttaagat tcttatggga gattttaata ccgatgcctt ctcagatcga
      601 gaggcctacg aagcgattac agagctcggt ctttatgatt catataatct agcgatggaa
      661 aaagaccagg ggattacggt tgaaaaggct atcgatggct gggctggtca cagtcaggaa
      721 aaacgattgg attacatttt cttaaatcag aaaaaggaag ttttatccag cttagtgatt
      781 ttcaatggag agaatcgtcc gattatttca gaccattttg gtgtggaagt tgaaattaat
      841 gtataaaatg gcacagagcc aaaaaataaa ggagaaaaaa tgaaaaaatt tcttagtttc
      901 gaattttggc aaaaattcgg taagtgttta atggtcgtga ttgcagttat gcctgcagca
      961 ggtctgatgg tttctatcgg aaactctatt ccgttaatca gcccagaatc agaactcttg
     1021 attcgtattg ggaatatcat tgcccaaatc ggttggggga ttatcggaaa ccttcacttg
     1081 ctctttgcct tggctatcgg tggaagctgg gctaaggaaa aggctggtgg tgccttctca
     1141 gcaggtcttg ccttcatcct tattaacttg attacaggtc acttctttgg agtgacgact
     1201 gacatgcttg cagatgcagc agcaaccgta accactgttt ttggaacaga gattccagtt
     1261 tcaggctact tcgtcaacat cttgggtcag cctgctctga acatgggtgt cttcgttggt
     1321 atcatcgctg gttttgtagg ggcaacaacc tataacaaat actacaatta ccgcaaatta
     1381 cctgatgttt tgaccttctt taacggtaaa cgctttgtac catttgtggt tatctatcgc
     1441 tcagttcttg tagcgctagg attagctatc ttttggccag ttgttcaaac aggaatcaac
     1501 agctttggta aatggattgc aagctcacag gatacagcac caattcttgc accttttgtt
     1561 tatggtacct tggaacgttt gcttcttcca tttggtttgc accacatgtt gaccattcca
     1621 atgaactata catcacttgg tggcacctat gacatcttga caggtgcaca agcaggtaca
     1681 caagtatttg gacaagatcc gctctggttg gcttggatta cagacttgat caacctcaaa
     1741 gatgctggcg atatggctca gtacaatgac cttcttgcaa acattactcc agcccgtttc
     1801 aaagttggtc aaatgattgg ttcatcaggg attttgatgg gattgactta cgctatgtac
     1861 cgcaatgtgg atgaagataa aaaacataag tacaaaggta tgtttatttc ttcagcactt
     1921 gccgtattct tgacaggtgt aacagaacca attgagttta tgttcatgtt tgcagcaatg
     1981 ccactctatg tcgtttatgc ttttgtacaa ggtgcggctt ttgccatggc tgacattgtc
     2041 aacttgcgta tgcactcatt tggtaatatc gaattcctta cacgtacacc gatggcgatc
     2101 aaagctggta tcggtatgga tgttattaac tttatctggg taacagccct ctttgcggtt
     2161 ggtatgtact tcattgccaa ctttatgatt aagaaattca acctagcaac agctggacgc
     2221 aatggcaact atgatacaga aacaacggat gtggtttcaa actcaaacgt agatactgcg
     2281 gatgccaatt cacaagtggt tcaaatcatc aacttgcttg gtggtcgtga taatatcgca
     2341 gatgtggatg cttgtatgac tcgtcttcgc gttagtgtta aagatgtggc acaggttgga
     2401 gatgaaaatg cttggaaaca ggctggtgct atgggcttga ttatcaaaga ctcaggtgtt
     2461 caagcagtct acggaccaaa agcagatgtt ctcaaatcag acattcaaga cttgctagaa
     2521 tctggcgtag ccattcctcg tacggaaatt gttgcgacag aagaagttgc tcctgagact
     2581 cagttcaaag gtgtgacgga gacagtctac tctgttgctg aagggcaagc cattgccatc
     2641 acagaagtga aagatccggt cttctcacaa aaaatgatgg gcgacggtta tgcagttgag
     2701 cctagctcag gcaatgttta cgcaccagtt tcaggtattg taaccagtgt cttcccaacc
     2761 aaacacgctg tcggtatttt gtctgacaag ggtgtagaag ttttagttca cattggttta
     2821 gacacagttg cactaaacgg tgctccattc tcaactaagg taacagatgg tcaacgtgtt
     2881 gaagcagggg atttgctcct tgtcgcagat ttggaagcca ttcgctcagc aggacgtgaa
     2941 acaactatcg tcgttgcctt cacaaacaca gctgaaatca aatctgtcag ccttgaaaat
     3001 cttggacagg tcagtcaaga tagccaagtt gcgacagtag agttgtaaaa agtaaaaaag
     3061 attgaaaaga tgattgcagt caactctttt caatcttttt cttttggata gtatgccatc
     3121 agtatttttc taatttcttc gcttcttttt tacctttttc agcgatatgg aggaaataaa
     3181 tagaccaagc aaggaaaaat aagggaataa tgccacttct aaacaatgcg aatggggttt
     3241 caaagagaaa accaaaccaa aaactggtac cagttcgttc ccatagtcct ggagttaggt
     3301 accaggattt aggattccaa agtgtgtatc cattttggag tccaacttgg taatggataa
     3361 aaaataaagt cagaaataat ccgaaatagg agtaggcata attttggtag cgtgaataga
     3421 atgctttttt cgattcggta tcagaaaaaa tttctgtgct tgaagtttcg attgtttgtt
     3481 gaaaataatg gatgccacat ctacgactac cttgtatagg ttgccaacca cagtcttcaa
     3541 atagagagag gtaatttgaa tattctcctt ttgcaatatg ttcatgaaaa tcaaggcgta
     3601 cgggatgagg ggttcgacta catttttcaa aatgataggt tgctgtccac ggagttactt
     3661 tgacaagctg ataaccttcg gattgaatcg aattgatcca atgttcttcc tcttgtaaac
     3721 tggtgaataa tttgtgtctt atcatggctt cctccttaaa aacctaattc tttggcaata
     3781 tgatgtagtt tttgaatacg ctgtgtttcc agtttaataa tctcttggcc cgttgctgtt
     3841 acttgataga cttttcttct tttatcggaa cttggaacag aacaaatcca ttttagtttc
     3901 aaaagattct caatcgctcc atacatggtt cctgcagcga ttttcacatc accatcactc
     3961 atatcctcta ctttttgcat gatatggtag ccatgggcag gttccaagag ggctaataga
     4021 atatagtagg ttgtctccgt taaggggaga tgcttgttcc ttttcatttg gtttctccta
     4081 tgtagttcaa atgtatagtt gtattatata ttatatatcg gggaactata catgtcaact
     4141 gtatagtgaa gaagatttta aaaatcttct caatccttgc catttatggt ataataaagc
     4201 gattttataa aatgtaggag gttccccatg ggacgtaaat gggccaatat tgtagccaag
     4261 aaaaccgcaa aagacggtgc taactcaaaa gtttacgcca aatttggtgt tgaaatctat
     4321 gtagcagcga aaaagggtga cccagatcca gaaacaaact cagcgctgaa atttgttatc
     4381 gaccgtgcta agcaagcgca ggttccaaaa cacatcattg acaaggccat tgacaaggca
     4441 aaaggaaaca cagacgaaac cttcgtggaa ggtcgttacg aaggctttgg accaaatggt
     4501 tctatgatta tcgttgatac tttgacatca aacgtaaacc gtacggcagc taacgtgcgt
     4561 tctgcttttg gtaaaaacgg cggtaacatg ggtgcatctg gttctgtatc attcatgttt
     4621 gataaaaaag gtgtggttgt ctttgctggt gatgatgcag atgcgatctt cgaattgctc
     4681 cttgaagcag atgtcgaagt ggatgacgta gaagcagaag acggcacaat cactgtctat
     4741 acagctccaa ctgatttgca caaggcaatc gtggcactta aagaatcagg aatccaagag
     4801 ttcaacgtca ctgaacttga aatgatccca caatcagaag tatcacttga aggcgatgac
     4861 ctcgcaacat ttgaaaaact ctacgatgcc ctagaagacg acgaagacgt ccaaaaaatc
     4921 tacacaaatg tagatgggtt ttaatataaa aaaagtgcta gtagcacttt ttttatatta
     4981 aaaggttgga gattggaatc gtcggtagac gattctcatt gtacgtggga cgtcctagaa
     5041 ggttggagat tggaactgcc gttaggcagt tttctttgta tccaattgct caaagacgat
     5101 agttggttgg aactagagaa gtggggatat tttagcttaa gtttgaataa ggtacgatgg
     5161 aaaaatttgc ggagttttcg gtggctgata gtagagtgct tgatagatga ggtataagga
     5221 aaaactatag ccaggctttg gaaatatcta tttgaatggt cacgggtttt ctgttatcat
     5281 agaacggtaa taagattagg agaatgtatg tcaaataatt tacttgtttt acaatcagat
     5341 tttggtttag ttgatggtgc agtttcagcg atgattggtg ttgctctcca agaatcgaga
     5401 gatttagttg ttcaccatct aactcatgat attactccgt acaatatctt tgagggttca
     5461 tatcgcctct ttcaaacggt cgaatactgg ccggagggaa caacatttgt ttcggttgtc
     5521 gatccgggag ttggttcaaa acgtaagagt gtagtagctt tgaccgaaca aaatcactac
     5581 attgtaacgc cagataatgg gacgctatcc ttcatcaaaa aatatgtggg gataaaggcg
     5641 gttcgagaaa tttcagaggt ggccaatcgc cgtgccaata cagaacattc gtacaccttc
     5701 catggtcgag atgtttatgc ctatacggga gctaaattgg cttctggaca cattagcttt
     5761 gaagaagttg gtccagaatt aagcgtggat aagattgtgg aaattccaac agtcccaacg
     5821 gaagttggtt cggactatgt aaaaggtgca attgatattc tggatgttcg atttggctcg
     5881 ctttggacct cgattacgag ggaagaattt tacaccttgc aaccgcagtt tgaagatcgc
     5941 tttgaagtga ccatatacaa taacgacatg cttgtgtatc aaaaccaagt aacctatggc
     6001 aagtcctttg cggatgtacg tatcggacag ccattgattt atatcaattc cctctatcgt
     6061 gtaggtgttg cgattaatca gggctcattt gccaaggctt ataatgtcgg tgtaggtcaa
     6121 aactggcata ttgaaatcaa acgtattagt aattaaagaa attttaggag atagttatga
     6181 aaaataattc aatcaaaaca gtcgttgcta ctggtatcgg cgcagccctc tttgtggtta
     6241 ttggtcattt aatcaatatt ccaacctttg tcccaaatac ttctatccag cttcaatacg
     6301 ctgttcaatc gctattggca gttgtatttg gtcctgtggt tggcttcttg gtcggtttca
     6361 ttgggcacac gcttaaggat tcgttgactt acggtccatg gtggtcatgg attttggcat
     6421 caggggtctt tggtttggtt gttggtctta ccaaaaaacg tctgcgtatt caagaaggga
     6481 tttttgaagg gaaggatatt cttttcttta accttgttca gattgcggcg aatgtgcttg
     6541 cttggggagt cattgcccca gttttagata ttcttatcta tagtgaggcg gcgaataagg
     6601 tctttgcaca gggacttgta gcaggtattg caaacagtat tacaattgct attgcaggga
     6661 cactcctttt ggttgtttat gcaaaatctc aaacgaagac aggtagtttg tctaaagatt
     6721 aagtttttat tttccctaga aaaaattcta gggttttttc aataccaaaa aatacatata
     6781 tgatataata atataaaaca aacaaaaaca tgtgttcaag gggagaagta tggtctggac
     6841 agaagaaaag ttgggaccga ttattcattc tatactcaat cctgacgagg aagttttaag
     6901 tctttatcaa tcgaaaacga cgaaatcaat ttatggatgt attctttgga ataattatta
     6961 ttataaggtt tttcgagtta gtaaccaccc atcacaatct acatataaac agccgacttt
     7021 ttatgatttc catggcgaat tttatatgaa aggagcgatt cggacctacc tttatcacac
     7081 gaacagttgg tatgaactta gtaaacaagg ctattacctt cttctctttt ttcaaaagtt
     7141 atgggatgaa aagcaagagg tggaggcgag ttggcaaaat ggtaggatgc agattcgctt
     7201 agtggttgaa gaagaggggt ggcaagttca ttaccgtttt ccagataatc aggcaaggga
     7261 ggttgaacgc ttattgggct caggcatgct ggtggccatt cgctcctcca gaaattttat
     7321 caagatacgg acgagtcgtt ttgttgcacc gtatatagct ctaatcaaac agcgtcaact
     7381 ttatcgaaaa aagccgagta aagctatgct ccagtgggaa aaataccaaa gtcaacttat
     7441 tgaaatccaa aggacctatc ggctagtgga ggaaagtgct ccgcaaactt tttttggaca
     7501 ctggttgaat aaggtccaac tctatctctg ctctgccatt gccagctatt gggaagaagt
     7561 tattaaagga gtggagtggc aggaggcaag agaaatcaaa gagcaaagga aagccgagaa
     7621 gccacataat cctattgaag ataaatggaa ggaaaatatt gctagacgcc ataagcgaaa
     7681 aatggaccaa cttatcggac atgatacctt ggaaaaactc cgacaactga aaacagaatt
     7741 agacgactaa gaagttatag cgaaccttca ataaatgcag tagcatgtca ttcatgttac
     7801 cgtatttttt tacatataag cactgaacct gcgaatacag gttctaaatt ttcagttcag
     7861 atttttgtct tttgaggggc taaacgatat aatacgtaag gtatcattta ttagaaatga
     7921 cagagtttta tcatctattg caaagattga aaaggagaca ctatgtcaaa agatattcgc
     7981 gttctactct attacaagta tgttccaatt gaaaatgcca aagaatatgc tgctgagcat
     8041 ctagctttct gtaagtctat cggactaaaa ggacgtattt tgattgctga tgagggaatc
     8101 aacggtactg tttctggtga ctatgaaaca actcaaaagt acatggacta cgttcatgca
     8161 aacccattgt tcagcgattt gtggtttaag attgatgaag aaaatgagca agctttcaag
     8221 aaaatgtttg ttcgttataa aaaagaaatt gttcaccttg gtttagaaga caacgacttt
     8281 gacaacgata ttgacccact cgtgacaaca ggtgcctacc tatcaccaaa agagttcaaa
     8341 gaagctctct tggacgaaga tacagttgtt ttggatacac gcaatgacta tgagtatgac
     8401 cttggtcact tccgtggtgc gatccgtcca gacatccgca acttccgtga gttgccacaa
     8461 tgggtccgtg ataacaaaga gaaattcatg gacaagcgcg tagttgttta ttgtactggt
     8521 ggtgttcgct gtgaaaaatt ctcaggctgg atggttcgtg aaggctacaa ggatgtcggt
     8581 cagctccacg gtggtatcgc aacctatggt aaagacccag aagttcgtgg tgaattgtgg
     8641 gatgggaaga tgtatgtctt tgacgagcgt atcgcggtcg atgttaacca tgttaaccca
     8701 gttgtcgttg gtaaggactg gttcgatggc acaccatgtg agcgctatgt caactgtggc
     8761 aatccattct gtaaccgtcg tattttgaca tcagaagaga atgagcacaa gtatgttcgt
     8821 ggctgttcag ctgaatgccg tgctcatgag cgcaaccgtt acatctctga aaacggcttg
     8881 actcgtcaag aatgggcaga acgcttggaa gcgattgggg aaactcttac tccagcaaat
     8941 gcttagtaat accaaaattg cattgaaaaa tcagtgcaat ttttttgtgc aatcagaatt
     9001 ctttatattt tttcagtcca ttctatacga agcctatcaa ttagaaaaga gaaggagatg
     9061 aagtcatttt ggatgaactt atccccttcg aaaatcaaca ttatccgttg tcaacttgcc
     9121 ttgatgaact tcagttctat cttcggcttc gtttcctcgg tcacttttga ttttcattga
     9181 gtattagcag tagcagacct tgaatagctg gtcggtttta tgaagcaatc ggttttgcag
     9241 ttgcgacgtt tggagatatt aagtctgcga ctttgcctaa gggtggcaac ttgattttga
     9301 tgcaggcaga tagctttgct caacgcgtac catttcaggt agtagcgagt ggcaatgaag
     9361 tcttgatttc gcttaatgtg gcgagtaggg aagaagtaga tagaattatc gagcgtgttg
     9421 aagcaaatgg cgggcaaatt acaggatgcc cgacagatgc ccgtggcttt tatggagcaa
     9481 gttttacgga ccttgatggt atcattttaa tgtgattgtg atgtaggaag ttagaattca
     9541 tctgctctag aatcatagat gaacttattt gaaaagggag aaactgatga ttcttccttt
     9601 ttttcagagt caatttacgc tataatagtc ctatgaaaaa aatcattgag tttaaagatt
     9661 ttacatttaa ataccaagcg caacaggaac ccactttaaa agatattcag ttaacgattt
     9721 atcagggaga gaaagttctg attattggcc catctggttc aggaaagtcg acgattggac
     9781 aatgtcttaa tggtattatt cccaatattt acaagggaga acatagcgga agtttactat
     9841 tggatggtca ggaagcattt aatctttcag tttatgataa gtccttgctc gtatcaacgg
     9901 ttctccaaga tacagatggt caattcattg gcttatcagt tgccgaggac ctagcttttg
     9961 ctttggaaaa cgacatggaa agccaggtgg tgatgaagca aaaagtagga gattgggcag
    10021 aaagactgtc tttgaccgat ttgctggcac atcgaccaca agatttatct ggaggtcaaa
    10081 agcaacgtgt cagtctgggc ggagttctta ttgatgagag tcctattttg ttgtttgatg
    10141 agccacttgc taatttagac cccaaatcgg gccaagatac gattgatttg attgaccgtc
    10201 tgcacaagca agaagggacc acaaccatta ttatcgaaca ccgtttagag gacgtactgt
    10261 atcgtcaggt ggaccgagtt gtattgatta atgatggtcg gattttgttc aatggaggac
    10321 cggatgattt attacgtacc tcattgttac aggaaaacgg catccgtgaa cccttatatc
    10381 tatcgactct tcgcgcctta ggatatccga ttgcgcaggc agaacgcctc tcttcagttt
    10441 gggaaattga tattgaggct ttgaatatag catccctacc gactctacac tttgaaaatg
    10501 gagagaaaga ggctttgtta gaagtcaagc atctttattt ttcctatcct aacaaacaag
    10561 ttcttacaga cattcaactg acgattcata agggggaacg tctagctatt gttggaaaaa
    10621 atggtgctgg gaaatctact ttagcaaagg tgctttgtgg ttttattcag ggtgagggtg
    10681 agattctctg gaaggggcag tcaatcaaag aagattctgt caaagagcga gcagaacgga
    10741 ttggttatgt tcttcagaat cccaaccaaa tgattagcca aacgatgatc tttgatgagg
    10801 ttgctcttgg tttgcgcatg cgaggggtga cagaagaaga aattgaacac cgagttcacc
    10861 atatcttgaa agtatgtggc ctgtatccgt ttcgtaattg gccgatttcg gcactttcct
    10921 atgggcagaa aaaacgggta acgatagcat ctatcttggt tctcaatcca gaaattattt
    10981 tgctagatga gccaacagca ggacaagatc aacgacatta tcgggaaatg atggagtttt
    11041 tggatcaatt aaatgctcaa gggcacacca ttgtcatgat tacccatgac atgcaactca
    11101 tgctagatta ttcagataga gctgttgttg tggtggatgg gcagattatt gaagatgcca
    11161 gtccagcaga aattttatct gatgatacgg tgattgagcg agccaattta aaagaaacat
    11221 caatttttca cttggcggag agggtgggag ttaatccctt agaattaacg acgttttata
    11281 tgcaggaaga aaggagagga agatgagtca acaaaaacta attggttacc atccaggaac
    11341 tggcttgatt catagtttat cggctgtcag taaattactc ttttttctca tagtttccat
    11401 tcttgctatg attacttacg atacccgctt gattcttttt atcgcagtat tttctctagc
    11461 cttgtttaaa atgtcaggga ttcgttataa agaaatctct cttgttttaa ttctgacaat
    11521 catttttgct gcaatgaatg ccctgatggt tcacttgttt gcaccacggt atggagtgga
    11581 actttacgga gcagacactc ccttgctatc aggtctggga gtttattcct tgacgagtca
    11641 gcaagcattt tatctggtga atcttctctt gaagtatttt tgtacagttc ctttagcgat
    11701 tatctttctc atgaccactc atcctagcca gtttgcttcc agcttgaatc aaattggggt
    11761 atcctacaaa gttgcttacg ccgtcagttt gaccatgcgt tatattccag atattcaaga
    11821 agagttttat acgattcgaa tgtctcaaga agcgcgtggt ttggaattgt ctaagaaagg
    11881 aaaattgatg gatcgtataa aagggaatct atcccttgtc attcctctga ttttcagctc
    11941 cttggagagg attgatacga tttcaacggc tatggaattg cgacgttttg gaaaaaataa
    12001 aaaaagaaca tggtataccc aacagcctct acagagaata gattacgctg tcttgctatt
    12061 tattcttgct ctagtagtgg tgacaatcta tctatttttt gttaatcagg ggcgattcta
    12121 taatccgtgg cgatgattat tgagtttatt ggacttatca accctttatc gttgacatat
    12181 aggaaatata ctgttacagt agggaatgta aataaattta gagtttaagt atcagtttca
    12241 tcttttgttt ttcctcttga taagttttac aaataaataa aaagaggtaa cacaaatggt
    12301 acaaggtaca gttaaatggt ttaatgcaga aaagggattt ggttttattg ctcaagaaaa
    12361 cggtccagat gtatttgcac acttttctga aattcagtct aatggtttca aatcgttgga
    12421 agatggtcaa aaagtgacat ttgaagtgga gcaaggccaa cgtggtcttc aagcaaccaa
    12481 tattacaaaa attggataat aaaagagcgg gtttcttagt gaaactcgct cttcttatgt
    12541 ttaaaggggt cttttatttg gcataccagc tttgcgtgga aacttatttg gagtttcctt
    12601 tttcttttcg acgatggtca atgttcgagg gtcgccattt ggaagagtat agtcgtggtt
    12661 ttcgatgact tttgcgaaga ggagattgag tgcgtttttt gcttgggtca attcatcttc
    12721 tgcgctggac gccttaagtg cgattaattt tccgtggagc ttcaaataag gaatagtcaa
    12781 ttcagaaaga atctgcatgc gggcaactgc gcgtgctgtg acaaggtcaa attgtgcacg
    12841 gaagttctta tcttgtgcaa agtcttctgc acgaccatga tagaaatgaa caccttcaag
    12901 ctctagttct tcggcaagca aatgtaaaaa gttaattcgt ttattaagag aatcaatgat
    12961 ggtaacatct agttgcggaa agatgatctt cataggtagg ctaggaaatc cagctccagc
    13021 tccaatatct aaaagccgaa gaggttcgtt ctgaatatgg ccttgcagga gtggagcgat
    13081 agaatcgtag aaatgcttca gatagacttc gctttcctcg gtaatagctg tcagattgat
    13141 tttttcattc cattccacta atagttggaa atagcgatga aattggtgtt tttgttggtc
    13201 agttaattga ataccttggt ctgccaaggt tttataaaat acttcaggtt tcataatctt
    13261 atcttatcat aatttaagca aaagaaaaac ccagaagagt ctgggaattt tcttattgac
    13321 catctgtctg tcccgttgcg ggattgtttc ctgttgttgc agtttcagtt gaagaagttg
    13381 cagtctcggt tgcagttcca gatgatgttt cgcttgatgt tgtcgtcgct tcggttgttt
    13441 cactagtttc agtcgaacta gaagaacttg tactagagct agtgtaagta ttataagcat
    13501 tggagagtga gcgactacct ctgagatagt agttggaacc gtaacgtacg agtccgcttg
    13561 gcatctccca atcggtagct gtatagtccg aatgcataaa gagcatcata ttgtggtaga
    13621 catctgttgc aattcgcatg ctgttatcaa ggataggagt catacgattg gtatatccgg
    13681 tccaaacagc cattgcgtac tgactagaat atccaacgaa gttttcatca ggaacgacca
    13741 tgtagctata atttgcttct ggaattgaag ctagaatagt atccgtttcg ctatcggtat
    13801 agtttgatgt acctgtttta ccggccatag gaacaccact aacagaagcg tttaatccat
    13861 aaccgtaaga cataacagcc ttcatcatgt cggtcatcat gtaggctgtt tcggcagaca
    13921 tgactttggt ccctttagga gcatattctg ttactgttcc atcactaaag acaattttat
    13981 tgacatactg tggagcgtag taagtaccac cattggcaaa agcagcgtag gcagcagcca
    14041 ttttttcact acttgctcca tacttactgc tagtatcgga tgtattactt gaaatagcat
    14101 tagagtaatg aatatctgga taattgatac caacgctatt caaaaatttc aaggcatttt
    14161 ctaagcccgt agcttccaat gctttaacgg cggtaacatt tcgagaatac tgaatggcag
    14221 tcttaacaga gatattaccg tagtattgtt tatcccagtt attgacagga gttggtgtac
    14281 caggatagtt ataaggaccg tctaagatta aatctgctgt agatgtataa atgccgtttt
    14341 caaaagcagg agcatagtct gtgattggct tcattgtaga accgaagtca cggttggttt
    14401 caacggcttg gttggtacca aatgagacgt ttgtttcttg tttacgaccc cctaattgcg
    14461 caacgacttt accattagaa acatccacaa ccgttgaagc tacctgcaat aaatcatctg
    14521 gatagctgac atacatatct gtattgtaga tattccaaag ttgctgctgg gcggcaggat
    14581 cgacgttcgt atagacatcc atccctgttg tcagcaagtt gtaacctgtt tttgcttcaa
    14641 cttcttcaac gacttgtttc agatagttgt ccatataagc aggataagca gctgcattag
    14701 atagtggttg caaaccatct gttacaggtg ttagaacagc ttgttcatac tgggtattat
    14761 caatgtactt ttcttccagc atctcagcca ataccaaatc gcgacgtgct tttgcatctt
    14821 ctggattggt ataaggatca tattggtttg gcgcttgggg cattcctgct aaaagagcta
    14881 attgaggaag agtcaaatct tttaactctt ttccataaaa agcaagagca gcagtcttca
    14941 taccgtaatt accgtttgac atatacacct tgttgatgta gtaggtcaaa atctcttgtt
    15001 tggtattgcg acgttccaat tgagtagcta gccaagcctc ttgaattttc cgttttagat
    15061 tttgatcttc tacggatgtc gagaaataag tcagcttgat aaactgctgg tcaagtgtcg
    15121 agccaccctg caaacgtcct ccgctcaagt tattaatcag ggaacctgca atacgaatca
    15181 cgtcaactcc tctatgattg aagaaacgtt ggtcttcgat tgcgacaatc gcatttacca
    15241 agtctgttgg aatttcttca gtcttggcac tcgaacgttt ttctgctcct aaatctgcaa
    15301 taagatttcc gttcttatca taaattttac ttgacacagt tgctgtcaaa gattcttctg
    15361 tcaattctgg agcgctttgg atgtagtaaa caaccaatgc agctccggcc aggcaggcaa
    15421 tgatagcccc cgcaagtaaa atatttgcag tgattagaag agctttttta attgttgtag
    15481 ttttcaaaag attcaccacc taatagattt ttttcaacga tttccagata tgggatgctg
    15541 gggaaacgcc ccatttgaat ctcaaagccg tattgtcgaa taaaggttag aggcatcgac
    15601 ttactaccca tgtctacttg ataaaattcg atcaaatgag tggctggcaa gagataggtt
    15661 tcttttaatg ttgaaaagtg aagaaggaca aaacaaatac caccttgttt aacaacctgt
    15721 ttcatgtgtt caatctgatg cgcatgaaaa tttttcatgg gcatggatgc tttttggcgt
    15781 gtctctttcg cttcaaaatc aatataatgt cttttaaaaa caccagagta gtctgtggta
    15841 gatgcctgtc gaaagtatgc ttcgacaatc ttagcacggc tccgtttggg gtaatcaact
    15901 tttacaattt gcacgggagt tggcttcttg tgaatgacag caatatcgtg cgcaaggtaa
    15961 tattgattgc tatcgttgat agcggcctca aaagacattc cgcgattagc aaagtttact
    16021 cgctgggatg aaataggact ggttctattg attttttttg acaccttatg aggataattg
    16081 accatagaac tccttcttga taaaataata tcagcatatt ataccataat tagcgaaaag
    16141 agtacaagaa atggatacta agagcctttt ggtaacaggt tatagacata cagacctggg
    16201 aattttttca gaaaaagatc ctcgactgca cattattaaa tctgcaattc gtaggaattt
    16261 tattcgtttt ttagaagagg gtgttagttg gtttatactg acaggtcaat tgggttttga
    16321 atactggtcc ttagaagtct tagaagattt gcgagccgag gggtatcaac tgagtattgc
    16381 aacaatattt ccatttgaaa atcatggcga acagtggaat gaggctaacc aagcaaagtt
    16441 agcacggttt aagcaggttg attttgtaaa gtatgcctat ccacgctatg aaaacccagg
    16501 gcaatttaga gactataacc agtttttact ggataatacg acaggttgtt atttgtttta
    16561 cgattctgaa aatgaaacca atttgaaata tttatatcac atggtcttaa agaaagaagg
    16621 atataataga aaaacgctta cctttgagga attgaatgag gaggctgaaa atttttcaaa
    16681 ttctgagtga tttgacttga tttttcccta ttttttttaa tataatagga gtagtgacgt
    16741 tttagattag ggagagagag atggcaagta ttaaatttac gacaaaagat attttcgagc
    16801 aagattttaa aattggtttt cgtggctatg atcaagatga agtgaacgat tttcttgatg
    16861 atattatgaa agactatgat gcttatgaag cgattatcaa ggaattaaaa ggcgaaattg
    16921 ctcgattgaa agcgcaggca gcaaattctc ccaaaacaac tcttcctact gaagaatcga
    16981 acgatgtatt gcgtacagaa cgtccatctt cagcgacaaa ctttgatatt ctccgccgtc
    17041 tgaatcgtct ggagaaagaa gtatttggaa aacaaatcgt tcaggatcaa gaatagaaaa
    17101 ttgtacacct aaactgacac tttattcgca gttattttct tgtgcacaaa tacattctgt
    17161 atttgtgtca gagatattcc ttgatttaaa gtggcttgct ttgtagatac aacttctaag
    17221 ataattaagg tgcaattttt ggataatcgc gtggtagttt tctaccatga ggaaagtcca
    17281 tgctagcact ggctgagatg ccagtagtgt ttgtgctagg cgaatcaata agcctaggga
    17341 cgcccttggg cgttacggcg gttaaaatgg ctaagtcttt tgatatgtca gagtagatct
    17401 gaaagtgcca cagtgacgaa gtttttatgg aaacgtaaaa aatggaacgc ggtaaacccc
    17461 tcaagctagc aacccaaact ttggtcgggg catgggatgg atggaaatga acagaaatcc
    17521 tgactgttta gacagtagac agatgattat cgaaggtggt agaacctagt accactggaa
    17581 caaaacatgg cttacagaaa attgcataaa ataggtagct agctctgcta gcttttattg
    17641 tagaggaaaa aaatgaaaaa acaatttgag ttgattgcaa cggcagcagc tggtctggaa
    17701 gcggttgtgg gtcgagaaat tcgtaatctc ggctatgaat gccaagtgga aaatggccgt
    17761 gttagattcc aaggagatgt aaaatccatt atcgaaacca atatctggct gcgttcagca
    17821 gatagaatta aaatcattgt tggacaattt ccagcaaaga cttttgaaga gttgttccaa
    17881 ggagttttta acttggattg ggaaaattac ttgcctctag gctgcaagtt ccccatttcc
    17941 aaggccaaat gtgtcaaatc gaaattgcat aatgaaccaa gtgtgcaggc tatttctaag
    18001 aaagcagttg taaagaaact gcagaaacac ttctctcgtc ccgaaggggt tcccttgcaa
    18061 gagatggggg cagagtttaa aattgaagtg tcgattttga aagatgtggc gacagtcatg
    18121 attgatacga caggttctag tttgttcaaa cgtggttatc gggtggaaaa aggtggcgca
    18181 ccaatcaaag aaaatatggc agctgctatt ttgcaattat cgaattggta tccagataaa
    18241 ccgcttattg accctacttg cggatcggga actttctgta ttgaggcagc tatgttggct
    18301 aaaaatatag ccccaggttt gaagagaagt tttgcatttg aggaatggcc gtgggttgag
    18361 gatcaattgg tggttgcatt acgcaaagaa gcacaagctt ctatcaaaac agatttggta
    18421 ttagatatta ctggatcaga tattgatgct cgtatgattg aaattgcgaa gaagaatgca
    18481 tttgcggcag gagttgagca ggatattgtt ttcaagcaaa tgcgtgtgca agacttgcgt
    18541 acagataaaa ttaatggtgt cattatttct aatccacctt acggagaacg tttgttggat
    18601 gatgaagcca ttgttaccct gtatcgagaa atgggggaga cttttgaacc actcaaaaca
    18661 tggtctaagt ttattctaac tagtgatgag ttgtttgaaa cccgttttgg acagcaagcg
    18721 gataagaagc gcaaactgta taatgggacc ctaaaagtgg atctttatca attttttggt
    18781 caacgagtga aacgtcaagt gcaagaagtg caaggatagg aggacatcgt gggagaaaaa
    18841 aatagtcatc atcttccttt ggatgaggag aaagttttag attttgaagt ggcgaaagat
    18901 ttaacaatcg aagaagcagt caaaaaacat aaggagattg aagcgggtgt gacagaagat
    18961 gatggtctct tggatcgtta catcaagcaa caccgcgcag aaattgaatc tcaaaaattt
    19021 gaaaccaaaa tcaatcatct tcctttagtt gaggtggctg atgaggaaaa aaatcaagga
    19081 catgaaagcg cggaagaggt agaagcaaat gagtcttctc ttacggaagt tagtgaggag
    19141 attgctccta ttgtagaaga actttcggta actccgatgg aaaccttgga agaaacagtg
    19201 attgcaagta ccgtagcaat ggaggggtta tcttcagtgg cagatgattc tagtttagaa
    19261 ttggaagaag atgaaaccga agacttggac catagtgagg gcgcagatag ggatcagaaa
    19321 aagaaatttt acttctggtc agcagtaggg ctttcaatga ttggtgtaat ggcgacggcg
    19381 ctggtttgga tgaattcggt taacaaatcg aatactgcga cttcttcaag ttcaacaagt
    19441 acaagccaga cgtcatcaac agcatcaagt tcgacagatg cgaatgtgac agcttttgaa
    19501 cagctctata atagtttctt tacggatagt agtttgacca agctaaaaaa ttcagagttt
    19561 ggtaaattag cagaactgaa agtgctgttg gaaaaattgg ataagaattc ggacagttat
    19621 accaaagcta aagaacagta tgatcatctt gaaaaagcca ttgcagccat tcaggccatt
    19681 aatggtcagt ttgataaaga agtcgttgtt aatggtgaaa ttgatacaac ggcgacggtt
    19741 aaatcaggag aatctctttc tgcgacaacg acagggatta gtgcggttga cagtctattg
    19801 gcatcggtcg tgaattttgg tcgttcacaa caggaagtcg cttcggctac ggtagcaagt
    19861 gaggctgcag ttacaagaaa ccaaggtgct gatgaaactg tatctactgg tgtaccagcg
    19921 acaacagaag tcgcttcaac aacggtttca ggctcaacaa ccgactttgg tattgccgtg
    19981 ccagctggtg tcgtcttgca acgtgaccgc agtcgtgttc cttataatca agcgatgatt
    20041 gatgatgtaa acaatgaagc atggaatttc aatccaggta ttcttgaaaa tattgtgact
    20101 atctcgcaac aacgtgggta tataacaggc aatcaatata tcttggaaaa agttaatatt
    20161 atcaacggca atggctacta caatatgttt aaaccagatg ggacctactt gttctctata
    20221 aattgtaaga caggctactt tgtcggtaat ggtgctggtc attctgatgc cttggattat
    20281 taaacaaaaa agcggtttat ctgcttcgga ttgaagaaaa agttcatttt ggacaatttc
    20341 cttcaatctt ttttctttgt gttgaaaata tagtcatttg aattaacttt gatacatcgt
    20401 cactttcggc ttgccgtact ctgcagaagt gacttgcttt gcaagttata tctccaacct
    20461 agcacagcca ctaggctgtg ctagcaacct gcacctcggt tccttgtctg aaagctaatt
    20521 cattcgacta taaaaaaact ctcagaaacg ccgaaatatc aatgtttcta agagtttaat
    20581 gatttgctat ctttcgcaaa cttcttcaat tttatatatt tttaggagtt tgtctacgtt
    20641 ctgagcggtg tatccgcttt ttttgttaga ttggttttct ttcaaaggca tctgtagaga
    20701 ttccctgaga aaggattagt ttggcccatt ctttagctga atgaaggctg tggtctttgt
    20761 agttaccaca tgattcaatg gttgtcccag gaacatcctc ccaagtgatg ccttctgcaa
    20821 tttcttccaa actcgacttg atgacttgag caatcttttc agagtcttgt tttccccaca
    20881 taatcatgtg gaaacctgtc cgacacccga aaggagagca gtcgattagc ccatcgatgc
    20941 gctggcgaat tagtttagct aatagatgct cgatggtatg tagaccagct gtatccattg
    21001 cattttcgtt gggttggata aggcgaatat caaagtttgt aataatgtct ccttttggtc
    21061 cgacttcttc agaaattaag cggatgtaag gtgcttttac aatagtgtgg tctagctcga
    21121 agctttcgac agtgacttct tttttcatgg tttcctccaa ataagtgatg aatttattat
    21181 accatatttg acaatttgga attgtgaaat cgtttcaaat atgataaaat agatacagat
    21241 tttgtgaaaa tcgaattata aaaaaagagg tagaaatatg aatatcatta cagtagtagt
    21301 gacacttgtt tctgctctca ttggtttagt attaggttac tttgccattt ctttgaagat
    21361 gaaatctgca aaagaaactg cagaattgac acttttaaac gcagaacaag aagctagtaa
    21421 tgttcgtggt cgagcagaag agcaagctga ggttattcta aaaacagctg agcgcgatcg
    21481 ccagaccttg aaaaaagaat tactcttgga agctaaggaa gaagctcgta agtaccgaga
    21541 agaaattgca gaagaattta agtcagaacg tcaagagttg aagcaaatcg aatcacgctt
    21601 gacagagcgt gcgaccagtt tggaccgcaa agacgataat ttgaccaaca aggaaaagac
    21661 tttggaacaa aaggaacaaa gtctgaccga taaagctaag cacattgatg agcgtgaaca
    21721 agaagttgta aaactcgaag aacaaaaagc tttggagtta gaacgagtgg cggccctcag
    21781 tcaagaagag gcgaaggaaa tcatccttac cgatacacgt gataaactga cgcatgaaat
    21841 tgcgactcgt atcaaggaag cagagcgtga agtaaaagaa cgttctgaca aaatggcaaa
    21901 ggacttgtta gcccaagcga tgcaacgcat atcaggtgaa tatgtggctg agcagacgat
    21961 tacatcggtt catttaccgg atgatgcgat gaagggacgg attattggac gtgagggtcg
    22021 aaatattcgt acctttgaaa gtctcacagg tatcgatgtt atcattgatg atacaccaga
    22081 agtggttgtt ctatcaggtt ttgatccgat tcgccgtgag attgcccgta tgaccatgga
    22141 gaccttgctt caagatggtc gtattcatcc agctcgtatt gaggagttgg ttgagaaaca
    22201 tcgtgtagag atggataacc gtattcgtga gtacggggaa gctgctgctt atgaagttgg
    22261 ggcaccaaac cttcatccag acttgattaa aatcatgggg cgtttgcatt tccgtacctc
    22321 ttatggacaa aatgttcttc gccactcaat cgaagttgct aaattagcag gtgtacttgc
    22381 tgctgaattg ggcgagaatg tgaatttggc acgtcgagct ggtttcctac atgacgttgg
    22441 taaagctatt gaccgtgagg tggatggaag ccacgttgaa atcggtacag aattagctaa
    22501 gaaatataaa gagaatccaa ttgttatcaa tgcgattgct agtcaccatg gcgatgtaga
    22561 agcaacaagt actattgcag tcattgtggc tgcggcagat gctctgagcg ctgctcgtcc
    22621 aggttcacgt cgtgaatcta tggaagccta catcaaacgt ctgcaagact tggaagaaat
    22681 cgccaacagt tttgaaggga ttaaacaatc ctatgctatt caggctggtc gtgaaattcg
    22741 gattatcgtt catcctaaca aggtcactga tgatcagatt accatcttgg cccatgatgt
    22801 tcgtgagaaa attgaaaata atctggatta tccaggaaat atcaaaatca cagttatccg
    22861 tgaaacaaga gcaacagatg ttgctaagta aatgtagaag agtccgaagg gctctttttc
    22921 tactggctca aagttcgttt tgggttggga atagaaaata gaaaatattt taatcgtatt
    22981 taaaagcagt tgaaattcat gctaaatttt gttacactag aatgaaagat ttaaaaggag
    23041 atatcatgaa agagcgaggc ttactcattg tcttttctgg tccatctggt gttggaaaag
    23101 gaacagttcg aaaggaaatt tttgaaagct ctgataataa atttgaatat tctgtatcca
    23161 tgacgacacg tccacagaga ccgggagaag ttgatggagt tgactatttc ttccgtagtc
    23221 gtgaggaatt tgaagatttg attcgccagg gtcaaatgtt ggaatatgca gagtacgttg
    23281 gaaattacta tggaacacct ctgacctatg tcaatgaaac tcttgataag ggaattgatg
    23341 tctttttaga aattgaagta cagggtgccc tccaagtgaa gaaaaaggtt ccagatgggg
    23401 tgttcatttt cttgacacca cctgacttgg aggagttgca agatcgcttg attggtcgcg
    23461 gaacggatag tgaggaagtt attgctcaac gtatcgaacg tgccaaggaa gagattgcct
    23521 tgatgcgtga gtatgattat gcagttgtga acgatgaagt tccgttggct gctgagcgtg
    23581 tcaagcgtat aatcgaagca gaacatttcc gtgtagaccg agtgattggt cgctacgatg
    23641 aaatgattcg tgaacagata gtagaacgat aaaagaaaga agagagaatc gcattatgat
    23701 gttgaagccg tcaattgata ccttgttgga taaggtacca tctaagtatt ctttggttat
    23761 tcttgaggct aagcgtgccc atgaattgga agcaggtgcc aaaccaactc aagaattcac
    23821 ctcagtgaaa tccaccctac gcgccttgga agaaattgaa tcaggtaatg tagtcatcca
    23881 tccagaccca gaagcgaaac gtgaggctgt tcgtcgccgt gccgaagagg ctaaacgttt
    23941 ggcggaagaa gaagagcgta aaatcaaagc tcaaattgct aaagaaaaag aagaaggcga
    24001 aaagatttag atacaagact cggttgggat tgccatccgg gtttcttttc actttcggaa
    24061 tgatgaaagt tttgacagaa aggaggcagg tcatttagtt tatcttttaa tatttgctcc
    24121 aactaatagt tgaaggttat acataaagtg aaccttgttc gcatcagtcg tgatattaga
    24181 aggtaaaata aatcgagatg gtatgttagc acagattatt gtagacgtcc ctttgatgca
    24241 gacagatcaa gcttattcct atcgagttcc agctgagttg gaagaggggc tcaaggttgg
    24301 cgtgcgtgtc catgtaccct ttggtaaggg aaatcggctg attcaaggga ttgtagttga
    24361 tttgattgat gaagatgatg aaaatagctt acaagacttg aaagagcttg ccgaggtttt
    24421 ggactatagt cctgttctca atcaagagca attttggttg gcggaccaga tgagaaagtc
    24481 ggtcttctcc tataaaatta ctctcttaaa atccatgcta cccagcttgc tcaattcgac
    24541 ctatgacaaa ttgatccgtc cagggattgg tttggaaatg gcagagcaga ttagtctgtt
    24601 tggtgataag gagcagattc gtttttctga cttggacgtg acagagcaag ggaaaatcat
    24661 gcgtctggcc cagtcaggaa aaatcttggt agactatgtg gccaaggata agaagcaaat
    24721 caagacagaa aaatggtacc gagtttgtct ggagaaatta cgagaagcag acatcagcaa
    24781 tcgggctaaa aaacggcagc agttaaggga atttcttctg gaacagtcgg aagaccagct
    24841 cctttcaaac ctaaaatcgg actattcagc tgatacgctc cgctatttcc aagaaaaagg
    24901 ctatatagaa gtctgggaag aggaagtgtc gcggactcag ggtgtttttg ataaggtaga
    24961 aaaaacgcag gccttggatt tgaatccaga gcaggccatt gcggttcggg agattgtagc
    25021 ctctatcggt caggaaagtc agacatttct actgcaagga gtgacaggtt ctgggaagac
    25081 ggaagtctat ctccaggtta ttgatagggt cttgaagatg gggaaaacgg ctattatgct
    25141 ggtgcctgaa atttccttga cccctcagat gaccaatcgt tttatttctc gttttggtca
    25201 gcaggtcgcg attcttcact ccggtctgtc tgatggagag aagtatgatg agtggcggaa
    25261 gattgaggcg gggaatgccc aggtggttgt cggggcccgt tcggccattt ttgccccttt
    25321 gacaaacttg ggtgttatca tcattgatga ggagcatgaa gcgacctaca agcaggacag
    25381 caatccacgc taccatgcgc gtgatgtggc aaagttgcgg gcagactata atcgagcgac
    25441 cctggtcttg gggtctgcta cgccgagtct tgaaacacgg gctcgggcta gtcgaggagt
    25501 ctatggaaga ctgactctta accagagggc caatcccttg gctcgtattc cagaagtgga
    25561 agttgtggat ttccgtgatt atatcggtca gcaggaggct agtaatttta cccctgttct
    25621 gattgaaaag ataagagaaa aattggctcg taaggaacag gtcgttctta tgttgaatag
    25681 gcgtggttat tcttcttttg tcatgtgtcg tgattgtggg agtgttgacc agtgtcccaa
    25741 ttgtgatatt tccctaacgc ttcacatgga tacaaaaacg atgaattgcc attattgtgg
    25801 ttttcaaaag gggattcctc agacctgtcc aaactgtcaa agtcgttcta ttcgttatta
    25861 tgggacgggt acgcaaaagg cctacgatga attgcaggag ctcttgccag aagctagaat
    25921 tttgcgtatg gatgtggata ctaccaagaa aaaaggtgct catgaggact tgctcgaacg
    25981 gtttggtcga ggcgaagcgg atattcttct gggaactcag atgattgcca agggcttgga
    26041 ttttccaaat gtgaccctag ttggggtgct caatgcggat acggctctga atttgcctga
    26101 ttttcggtct tctgagcgga ccttccagtt gctaactcag gtagctggtc gggctggtcg
    26161 agctgataag gaaggagagg tcctgatcca gacctataac cccaatcatt atgccattgc
    26221 ttttgccaaa gagcaggact atgaaggttt ctatcgttat gagatggaca ttcggaaaaa
    26281 tcttggctat ccaccttact attttacggt gggtctaacc ttttcgcaca agtcagaaga
    26341 atttgtcgtg aaaaaagcct atgaaactgt agcctttctc cgtcaacact taacagatgc
    26401 aattcaaatc ttgggaccga cgcccaagcc tattgccaga actcataatc tctaccacta
    26461 ccagatcatc ttgaaatacc ggcatgaaga ccgtttggaa gaggtattga atcagatttt
    26521 ggattggaca caggagagag aaaatcaaga tttgcgactg attattgaca atgaaccgca
    26581 gaatatgatg tgaggtcact attgaccttg catttttttg ttttaagaac ctttgattaa
    26641 ggaaaaaata agcaaaaatg atataattag gggatagtat aattgatatg aatcagaaaa
    26701 tgaaaaggag atgagaacac gaatatgaca aagttgattt ttatgggaac gccagcgttt
    26761 tcagcgacgg tcttaagagg gctcttagct gatgggaatt ataacatact ggcagttgta
    26821 acccagccag accgagctgt tggacgaaaa aaagtcatcc aaatgacacc tgtgaaagaa
    26881 gtagccttgg aatacaattt accagtctat caaccagaga agctatctgg aagtcaagaa
    26941 atggatgagc tgatgaattt aggagcagat gggattgtga cggcagcttt tgggcaattc
    27001 ctaccaacca aattattgaa ctcagttgac tttgcggtca atgttcatgc ttctcttctt
    27061 cctaaatacc gtggcggtgc ccctatccat tacgctttga ttaacggtga tgaacgtgcc
    27121 ggtgtaacca tcatggaaat ggtcaaggaa atggatgccg gtgacatgat ttccagcgat
    27181 agcattgcta ttgaagaaag cgacaatgtc ggcaccttgt ttgaaaagtt ggctgttgtt
    27241 ggtcgagatt tattattaca aaccttacca gcctatattg ctggtgactt gaaaccagtc
    27301 gctcaaaatc cagaacaggt aaccttctca ccaaatatcc agccagaaga agaggtactg
    27361 gattggaata agacagctcg tcaactattt aatcagattc gcggtatgta cccgtggccc
    27421 gttgcccaca cctattggca aggcgaacgg tttaaaattc aagaagctgt agaagcagaa
    27481 ggtgaaggat cagttggtcg ggttattgct cgaagcaaga aagaattgat cattgcgaca
    27541 ggtcagggag ccctttctct gaaaactgtc cagccagctg gtaagccaaa aatgaccatt
    27601 gcagactttt taaatggtgc aggtcgagat attgcagtag gagatcaatt tggtgatcaa
    27661 taaacatgaa acagcaagaa gtctagcctt atctgtattg gaacaagtct ttgatcaagg
    27721 tgcttactcg aacatcgctc tcaataaggc tctagaatct tctcgtttat cagcacagga
    27781 caagggcttg gcgaccgaat tggtttatgg gacggtttct cgtaaaatta ccctagagtg
    27841 gtatttggca cattttatcg aagataggga aaagttggac acttgggtct actatctcct
    27901 catgttgagc ctctaccagt tggtatattt ggacaaattg cctacccacg cagttgtcaa
    27961 tgaggcagtt aatattgcca agcgaaagcc tggaactgac aaatttgtta atgccatttt
    28021 gcgtaagctt agtcaggatt ccttgccgaa accagcagat attaaaagaa agaacaaacg
    28081 tctatccgtt cagtattctg tgccagtttg gttggtccaa accctgattg cagaatatgg
    28141 cgatgaacgt gcagagaaga ttttccaaag tttacatgat cggaataagg ccagtgtacg
    28201 tgtgacagat ggtcaacaag tggagcgatt ggcagaggag ctaggcgctc aacactcgca
    28261 gttatcacca gttgggttgg ttaagcctca tggacacttt gcggggacag attatttcaa
    28321 ggaaggactc atcactattc aagatgaaac cagtcaattg gtagctccga ctttggatat
    28381 tcaaggagat gaaatcatct tggatgcctg tgcggctccg ggtggaaaga cctgtcatat
    28441 ggcaagctat cttacggatg gaaaagttta tgcgttggat ttatatgatc ataaactaaa
    28501 cctgattgaa gaaaacgcac gtcgtttagg cctagcagat aagattgaga ccaaaggtct
    28561 ggacgccagt cgtgttcatg aggaatttgg tccagatacc tttgataaaa tcctagtgga
    28621 tgccccttgt tcaggtattg gactcatccg ccgtaagcca gatattcgtt ataataaggc
    28681 atcgatggat tttgattctc taaagactat ccagttgcag atactggaca gtgtttgcca
    28741 aagcttgaaa attggtggta taataaccta tagcacatgt acgattattg ctaaggaaaa
    28801 ccaagaggtt attcaagaat ttctgcaaaa ccatcccaat tttgaacagg tgacactaga
    28861 acaccctcaa acagatatta tggtggatgg ttgcttgttg ataacccctg aacagtataa
    28921 gacagatggc tttttcatcg gtcaacttag acgaaaatcc tagaataaga ggaggaagtt
    28981 gcttagcaac gataaaaatt atggaaattg cattacttac tgatgtagga cagaaacgtt
    29041 cgaacaacca agactatatc aatcgctaca aaaaccgcgc agggattgat ttggtcgtgt
    29101 tagctgatgg aatgggtggt caccgtgccg gccatattgc tagtgagatg gcagcaacag
    29161 atttgggagc tgcttgggtt gatactcaat tagtgacttt gaatgatgtc cgtgaatgga
    29221 tggttgcaat cattgatgct gaaaaccaaa agattcatga acttggacag acagaagaat
    29281 acaaggggat gggaacaact cttgaggcag ttgttgtcat cgacaatcaa atgatctatg
    29341 cccatgttgg agattcacga gttggattga ttagagatgg cgaatataca cgtctaacta
    29401 acgaccattc gctggtaggt gcccttgttc gagctggtca gctcacagaa gaagaagcac
    29461 agcgtcatcc tcaaaaaaat attgtaacgc agtccattgg acaagccgaa ccaattgaac
    29521 cagacattgc tttgaaaact cttgaggttg gagattatgt ccttattaac agcgatggct
    29581 taaccaatat gataccaacc gaggacattc gtgatattgt attgagtgat gtttcaattg
    29641 aaagcaaggt agaaacactt gtacgttttg ccaataatgc aggcggtcta gataatatta
    29701 cagtaggttt gcttcacatt acggaggagg ctaggtaatg attcaaatcg gtaagatctt
    29761 tgccggtcgg tatcgaatcg ttcggcaaat tggtcgaggc ggtatggcag atgtttacct
    29821 agcaagagat ttgattttag atggagaaga ggttgctgtt aaagttcttc ggaccaacta
    29881 tcagacagat caaattgcta tccagcgttt tcaacgtgaa gcgcgtgcca tggcagaact
    29941 tgaccatccc aatatcgttc gtatttcgga catcggtgaa gaagatggtc aacagtatct
    30001 ggctatggaa tatgtcaatg gcctcgattt aaaacgttat ataaaagaaa atgcaccact
    30061 ttccaacgat gttgcggtgc gaattatggg acagattctc cttgctatgc gcatggcgca
    30121 cacgagagga attgtccatc gagatttgaa acctcagaac gtccttttga cttccaatgg
    30181 agtcgctaaa gtaacagact ttggtattgc agttgccttt gctgaaacga gtttgacaca
    30241 aaccaactcc atgttgggtt ctgttcacta tctttcacct gagcaggcgc gtggttcgaa
    30301 agcgaccatt caaagtgata tttatgcgat gggaatcatc ctttttgaaa tgttgacggg
    30361 tcgcattccc tatgatggag acagtgctgt gacgatcgct cttcagcatt tccaaaaacc
    30421 tctcccatct gttagagaag agaatgccaa cgtacctcag gccttagaaa atgttgtact
    30481 aaaagcaacg gcaaaaaaat taaacgagcg ctataagtcg gttgcagaaa tgtatgcaga
    30541 tttagcttcg gctctatcca tggatcgtca gaatgaaccc agggttgaac tagaagggaa
    30601 caaggttgat acaaaaacct tgccgaaact ctcccaagcc aatgttgaaa cgaaagtacc
    30661 gcatacaaac agttctgctc aagtttctgc gacggataag gggtctggta agaaagaagt
    30721 ggcgaagtcg ggaaataagc ctgtttcaaa accaaggcca ggcatacgta cacgctacaa
    30781 agtcttgata ggagctattt tgttaacagt catagcggct ggactcatgt ttttcaacac
    30841 tccacgaaca gttacagtcc ctgatgtatc tggtcaaacc gttgaaaaag ctacagaaat
    30901 gattgaagtg gcaggattgg aagttggaaa tatcacagaa gaagcgacag cgactgtaga
    30961 tgaaggcttg gtaatacgga cctctccggc tgcaaagacg accagacgtc aagggagtaa
    31021 gattgatatt gtagtagcaa cggctgcgct ggcatctatt ccagatgtgg tggataagga
    31081 gtctgatact gcgcggcagg aattggaggc ccttggtttc caagtgacaa taaaagaaga
    31141 atatagcgaa aaagttgccc aaggactcgt tatcaaaaca gatccaggag caaatagttc
    31201 tgctgagaag ggtgcaaaaa ttacccttta cgtttccaaa ggagtggcgc cacaagttgt
    31261 tccaaacgtt gttggaaaaa gtcaggagaa tgctactcaa attcttcaga cagcaggctt
    31321 cagcattgga accattactc aggaatatag ctcatctgtt actgcaggtc aggttatcag
    31381 cacagatccc gttgccaata cagagttggc aaaaggttct atcatcaatc tagttatttc
    31441 aaaaggaaaa gaattgatta tgcctgattt aacatctggc aattacacct attcacaggc
    31501 gcgtagtcaa ttacaagcct tgggtgtcaa tgcggaaagt attgaaaaac aagaagatag
    31561 aagctattat tcaacgacaa gtgacatcgt cattggacaa tatcctgctg ctggagcaac
    31621 aattgatgga acagttacct tgtatgtctc tgttgcatca accagaacca gtagcgattc
    31681 ttcagcagga agttccacaa gtacaagtac ttcaacaggt agcggacaat aaagatgaat
    31741 aaggttgggg agatacccaa tgtcattaag ttatctaacc tatcacattt tgtgatgggt
    31801 tagttttttt atgttacact aaaaataaaa ggggaaaagt catgctagat caaattaaag
    31861 ctcatttata ctcttcgaaa atcaaaatta tccgttgtca acttgccttg atgaactcca
    31921 gttctatctt cgacttcgtt tcctaggcta cttttgattt tcattgagta ttacttgata
    31981 gtatagacaa tatttagttt tatagccctt tagtttctct ttgattactt catcaacttg
    32041 ctttgaacta aaagtaaact aaaactatat atcagtcagt cctatatttc ctactttgga
    32101 tggagttctt ttacttattt tttgttacaa tagtatagaa aggatggaga agatgcggaa
    32161 agttcaattt tttctactaa tagaaagtat gatttttacg atggcagcct ttgatgtggt
    32221 agccaatgag gccagtcgga ccctgctact tttctctgcc ttattactcg tcatttggta
    32281 cttcctagga agaaaagtca acagtgtttt actggtcagc tccttatctc tattattttt
    32341 ggtctttgtt ttaaatccct tcttcattat tgggattatg ctactagtcg tgtacatgct
    32401 gattaatttc ttttcacgtt atgaaaagag aaatcaatat acccacattg tttttagtga
    32461 ctattctcta caggttcaaa aagagaaaaa tagatggttt ggcaaccacg accactctca
    32521 agataggttt ggttttgaag atattaacat tgtacgcttg tttggcaatg atgtagttga
    32581 tttggataag actgtattag ttggcagaga taatattgtt gtaattcgta aaacgtttgg
    32641 acggacaaaa attattgttc caattgatgt tgaggttgca ctttcagcaa ctagtatcta
    32701 tggacgagta agtttcctgg gtgaatcata ttgggatttg cggaatgaaa gtatagcggt
    32761 caatagtccc gattatcaag aatcacacaa acgtgtaaaa gtcgttacca acaatatact
    32821 gggagatgtg gaggtggttc gcgtatgaga aaacgtacct acctactcct cgcctggttt
    32881 gcctgtctta ttgtttttgt ggtgattgct tccaccctcc cacttttaaa ttattcattg
    32941 ctagatgtta cgctctggat ttcaacggag cagctcgtgt ttactctcat tctactagtt
    33001 attgttttga ccctttttct agctgtaatg gtacaaactg tcagcctagc ctccactcag
    33061 gttatgcgga cgaagttaca agctatcttg aagaataaaa gtattcgaac agataccgaa
    33121 agtgatcagc tcttgctcca gttgtctgat aaggtcagaa acctcacgcg tcaggtgcag
    33181 ctggttgaca atcaagattt agtcaagaaa gaagagattg ttgagggaga acggaaacgg
    33241 attgccaggg atttgcatga tacggttagt caagagttat ttgcaacgag catgattttg
    33301 tcaggattat cgtccaatct atccactctt ccgcaggaaa ccttgcagga acagctgatg
    33361 ctcgtgaaag acatgattga gtctgcccaa agagacctac gcatcctatt attacatcta
    33421 cgcccaagtg aacttgaaag taaaaccttg gttgaaggat ttgagttgat tttgcgtgaa
    33481 gttagcgaca aaagtaatat tatcgttcat ttccaacatg aagtagagga gttaccgaaa
    33541 ctaattgaag aacatctctt ccgcattgcg caagaaatta tcagtaatac cttgcgacat
    33601 gccaaagcca agcatttgga tgtttacctc ttgcagaagg aaacagaact tcagttgaaa
    33661 atgacagacg atggtatcgg cttccaacaa ggtgcagatg atgaattaag ttacggtctt
    33721 caaaacatac gtgagcgggt tgaagacatg gctggaacga taaaaattcg cacagctcca
    33781 aataagggag tggcgatcga tattcgtatc ccactattga aaggaaaaga agatgaatac
    33841 gattcgagtg atgctggttg acgaccatga gatggttcgc ctaggattga aaagttactt
    33901 aaatttacag ccagatgttg aagtagttgc agaagctagt gatggcgagg aaggtttggc
    33961 taaagcgtta gaggttaaac ctgatgttgt agtgatggac ttggttatgc ccaagatgac
    34021 tggcgtagaa gcaactctgg ccttgctgaa ggaatggccg caagcgcaga ttgtcatcct
    34081 gacctcctat ctagataatg agaaaatcta tcctgtgctg gaagcaggag ctcgtggcta
    34141 tatgttgaaa acgtcaagtg ctgacgaaat cttagcagcc atccgcaagg tagctcttgg
    34201 agaatatgct attgagacag aagtagagaa aaaagtagag caccataaac gccacccaga
    34261 tttacatgat gacttgacag ctcgtgaacg cgaaatcttg acgctgttag ccaagggata
    34321 tgataatcaa cgaattgcag atgagtcctt catatcctta aaaactgtta agacccatgt
    34381 ttccaatatc ttatccaaac tagctgttag cgatcgtact caagcagttg tttatgcttt
    34441 ccagcatggt ttggtggcgc aagaggacca atagtggtat aatgaaaatt agttaatttt
    34501 taaccaaagg atagtggaat tatatggatg caaaactcag atataaggca aaaaaaataa
    34561 aaatagtctt ttttgacatt gacgacacgc tacgtgtgaa gaatacaggc tacattcctg
    34621 aatcaattca gcaggttttt aaatcactga aagaaaaggg aattttgaca ggaattgcgt
    34681 caggacgaac gccttatggt ttggtgccag aaatcaaggc cttaaagcca gactttttcg
    34741 ctatgattaa cggctcctac gtagaagatg ccaaaggtca ggtagtgtac catcagccga
    34801 tgccccaaaa tttagtagaa tctgtattaa attgggctaa agaaattgga atcgaatatg
    34861 gtatgcttag tagtcaaaaa gggacacttt ctgcacgaac tgaccgtatt agtcaagtaa
    34921 ttgacttgat ttatgaaggt ttggaaacaa aaccaacttt ttataaagaa aatgatatct
    34981 atcaattgtt aactttcgag aaagatggtc acgaagtaga acttccagaa gagttgcaag
    35041 cagaattgcg tagtgttcgt tgggatgcta tttcatcgga cattgtgtta aaaggctctt
    35101 ccaaagcaac tggtgttgct aaggttgttg aaaaactagg cctgaaacct gaaaatgtcc
    35161 tagtgttcgg agatggactc aacgatattg agttattcga ttatgcagga attagtatcg
    35221 ctatggggca ttcgcatcca gaattgcaaa agcatgcaga ttatatcaca aaaaaagtag
    35281 aagaagatgg catttttgat gccttggaga aattaggtat ggtagaaaaa gaaaaatatt
    35341 tcccgcaatt agatttggaa aatgttacag gaccagttgc gcatatcaag actaaccatg
    35401 gtaagttaac agtaaaactc ttccctgaaa ttgcacccaa aactgtagca aatttcgtag
    35461 ctctttctaa agatggctat tatgacggaa ttatcttcca tcgtattatc aaagacttta
    35521 tgattcaagg tggcgatcca accggaacag gtatgggtgg tgaatctatt tatggtactg
    35581 cgtttgaaga tgaattttca atggaagctt ttaatctcag aggagccttg tcaatggcca
    35641 atgcaggacc aaatacaaat ggaagccaat tctttatcgt tcaaaaccaa aacttcccat
    35701 acaatgcgaa agaattagaa cgaggcggtt ggcctaaaga agttgcagaa gcctatgtca
    35761 aaaatggcgg gacaccatat ctggatcaac gccacacagt gtttggtcat ctagttgatg
    35821 aagaatcctt cgtcgtcttg gatgctatcg cagcggttgc aacagattca gcagattgtc
    35881 cacatgaaga tgttgtaatt gagaccatcg aaatagagga ttaagatgaa aatcggagat
    35941 aaactcaagg ggacggtaac gggtattcaa ccgtatggag cctttgtaca attagagaat
    36001 gggacgacgg gcctaatcca tatctcggaa atcaaaacag gttttgttga taatatttat
    36061 gatcatttaa aattggggca agaggttttg gtacaggtag ttgattttga tgaatatact
    36121 gaaaaggcta gtttatcaat ccggacctta gaagaagaac aacaaaaaat tccgtgccat
    36181 catcgtttta caaatgaacg gaataaaatt ggtttttccc ccttagcaaa acaacttccg
    36241 aaatggattg aggaagctaa aggctatctc aaagaacaca aagaaaagaa ggtataggat
    36301 gcattcctaa ccttctttct ttttaatcat tgaactcata aagagctgtt gacaagtaac
    36361 gttctccgtt atcaggcaag attgccaaca cttttttccc agttcccaac tccttagcga
    36421 cttcgatagc agcgtgaatt gctgcacctg aagaaatacc aactaagaat ccttcttttc
    36481 caccgatagc acgacctgta gctaaggcat catcggattt gacacgaata atgccatcat
    36541 aggcgctagt gtccaaagta tctggaataa aacctgccga aataccttgg atcttgtgcg
    36601 gaccaggtgc ttcaccagat aagacagctg attcatcagc ttcaacagca tagatagcaa
    36661 tatctggatt tgctgtcttc aaaacatgcg aaacacctga aacggtacca ccagtaccaa
    36721 cgccagatac aaaggcatct aaaccagttg tgccaaagtc ttccaaaatt tcctgtcctg
    36781 ttgtatcttc gtgaactttt ggattggatg gattagcaaa ctggaaagga acccagccat
    36841 ttttttcttc agcaatctct tttgctttgg cgatagcccc cttcatccct tcgctaccag
    36901 gagtcaagac aagttcagct ccataagctt ggataatctt acgacgctca acactcatgg
    36961 tttcaggcat gacgataatc acattataac cttttgctgc tccaacccaa gcaagaccga
    37021 taccagtgtt accacttgtt ggctcaacaa tagtatctcc aggtttgatt gtaccagcct
    37081 tttcagcatc ttcaatcata gccaaggcga tacggtcttt tacagaagat cctgggttaa
    37141 aggcctctaa tttcacatag acctccgctg ccccctctgg aacgatatta ttgagtttaa
    37201 taacaggtgt ttttccaact aattgagtaa tgttttgata aatagccata tggcacctcc
    37261 aaataatgtt attatacatt atatagtaga aagtcaaaaa aggataatat aaaaaaacta
    37321 tctcgccgat agcttttttt aatagacagg tacctcaacg attcgagagt tagctttttc
    37381 cgtggatact tttccgtggt aaaagtcagt gagacttgct aaggtttgtt caaggaattc
    37441 tttatctaca aaaatcatgg ttgaaacttc agtagtaaac tgggtatcaa attcttccag
    37501 accttgctcc gctcgccaat tggcaaactc ttggtattgg gcataggaca tgctaaggga
    37561 aatcccttcc tgctccttaa cttccacaac cccgatttcc ttgactgcat tagccacggc
    37621 tccagcgtaa gcacgaataa gtccgcctgc cccgagtttg atacctccaa aatagcgggt
    37681 aaccaccgtt gcgacattgg tcagttcgtg attttctaaa accgtcagca ttggcacacc
    37741 agcagtacca gacggctccc catcatctga ggaacgcttg atttccatat tttctccaag
    37801 gacaaaagca gagcagttat gagtagcctt gtaatgctct ttcttgattt tgttgataaa
    37861 gtcgcgagct tcctcctcgc tggtcactcg tttcataaag cagataaatc gtgacttttt
    37921 aatttcttct tcaacaatac catcttctct aattgtttta aattctttca taaccaaatt
    37981 gtactaattt ttttaaaaac tgacaagaaa ttccgaataa ataagtgatg aaagaattag
    38041 aaaattatta tggaagatta tttaccaaat accaattgac agcaaaagaa agagaaatag
    38101 cagaaaaagt gccaagtatt acaaaaaaga ataactgctt tcgctgtgga acaacttttg
    38161 aagaagaaaa caaattgcca aacgatgctt attactgtcg agcctgcttg cttctaggca
    38221 gagtacggtc agacgaaaaa ctctatcatt ttcctcagaa agattttcca atcactaagt
    38281 gtttaaagtg gaaaggtcaa ttaactgatt ggcaacaaag aatttcagat ggactagttg
    38341 caaacgtgga aaataatcgt gcgacattgg ttcatgcagt aacaggagca ggtaagacag
    38401 aaatgatcta ccacaccgtt gcctcagtga ttgataaagg cggagcggtt tgcctagcca
    38461 gtcctcgaat tgatgtttgt atcgaactct ataaacgtct gcaaaatgac ttttcagttc
    38521 caattagttt actacatgga gagtctgaac cctatttccg aaccccatta gttgtagcaa
    38581 ccacacatca gttattaaaa ttttatcagg cctttgattt ggttttgatt gatgaagtag
    38641 acgcctttcc ctatgcagat aatcccatgc tctatcgagc agcagacaat gcggtcaagg
    38701 aagctggtgt tcaagttttt ctgacagcga cttcaacaga tgaattggat aaaaaagtca
    38761 gaacaggtaa attaagtcgt cttagtttgc caaggcgctt tcatggcaac ccacttgttg
    38821 tcccgcaaaa agtctggttt agtaaattcg atgataccct aaagaaaaat agactagttc
    38881 caaagttgaa aaaagcgatt gaagaacaga gaaagtcgtg ctttccctta ctcatttttg
    38941 tcccagaaat ctccaaaggt caagaattta ccaagataat gaaaaaaaca ttcccagaag
    39001 aaacaattgg ctttgtatcc agtcaaacag aaaatcgcct tgaaatagtt gaagggtttc
    39061 gcaagagaga aatcacagtc ttaatctcga ctactattct tgaacgtggg gtgaccttcc
    39121 catgtgtaga cgtctttgtt gttcaagcca atcattacct ctacacagtg tcaagtcttg
    39181 ttcagattgc aggccgagtc ggaaggagta tggaacgtcc gactggttta ctccagtttt
    39241 atcatgaggg aagtacagga gccattgaaa aggcagtcgc tgaaattaaa cagatgaaca
    39301 aggaggctgg ttatgtctaa ttgcctattg tgcggtcaag ctatgaaaaa taaaacaaga
    39361 ttttcagatc tcatcttctt tagtaaagaa aaatcgggca tctgtgaaga atgtttttcg
    39421 acttttgagg aaatagcaga gcaacactgt ccacattgtt ataaaaatgg ggaatcggaa
    39481 agttgcaagg attgtcagta ctggcagaat caagggaaac cagttgtcca cacagctttg
    39541 taccaatata atcaagcgat ggcgcattat tttagtcgct acaaatttca aggagactat
    39601 gttttaagaa atatttttgc taaaaagctt agaatagcgt taagccaatt tcctgactac
    39661 acaattgttc ccataccaat tagtcaaaaa cgactatcag aacgcggttt taatcaagtg
    39721 gaaggacttt tggatgctac aaatattccc tatcagtccc ttcttggaaa gtatgaaagc
    39781 caaaaacaat catctaaaaa tcgtgcagag agactagaag caaaacagat gttctatcta
    39841 ttagatgaaa aagaagtgcc agaaaaaata ttattattcg acgatatata tacaacagga
    39901 gccacaatcc aacttgctgt agaacttttc atgaaaattg gtaggaaaga aatcaaaaca
    39961 ttttcactaa cacgataaga aaacgattgc aatatcccta taatgtggta taataatagt
    40021 gaagaaaggc gaaagccaag aaagaggtac taatatgatt aaattcagta ttcgtggaga
    40081 aaaccttgaa gttacagaag cacttcgcac ctatgtagaa gagaaagtgg ctaagattga
    40141 gaaatatttc aatgaagaac aagagttgaa tgctaaagta aatctaaaag tttaccgtga
    40201 caagcgtgca aaagtagaag tgacaattcc agttggagca gttacacttc gcgcagaaga
    40261 tatttcacaa gaaatgtatg gctctatcga tcttgtagta gataagattg aacgccaaat
    40321 tcgtcgtaac aaaaccaaga ttgaacgaaa gcatcgtcaa aaagtggcaa ctggtcaagt
    40381 ctttacagat gaacttgttg agcaaacagg cgaggaagta aaagtggttc gtactaagca
    40441 agtagacttg aaaccaatgg atatggaaga agcagtcctc caattggagt tgctcggaca
    40501 tgatttcttt atctatacag atgctaatga cggtacaaca aatgtattgt atagacgcga
    40561 agatggagat ttgggtcttc tagaggtacg tcaataaaga taataaaaca gttgcagttt
    40621 ttgcagctgt tttatcattt tcaaaaaacc ggttgacaat gtatataagt c
//
LOCUS       DBNFCK010000004        34053 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.004, whole genome shotgun sequence.
ACCESSION   DBNFCK010000004 DBNFCK010000000
VERSION     DBNFCK010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 34053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34053
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.004"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..346)
                     /locus_tag="AC1PIW_000183"
     CDS             complement(<1..346)
                     /locus_tag="AC1PIW_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605157.1"
                     /translation="MRSHLGVRLSYEAKYWLESIQAVIQEKLDAKINEQDIENLEHAT
                     KSYLKEVDNELGATSVTLILKASASSVLEEAFEKTKSLSLKDWHKLDNEMKHSISSIP
                     KDKDVGTLSVRFF"
     gene            716..1498
                     /locus_tag="AC1PIW_000184"
     CDS             716..1498
                     /locus_tag="AC1PIW_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HIP6605158.1"
                     /translation="MKTLYHYTTVETLLLILNYKTLRFKSLLYVDDPLEPSTSDFGNL
                     GGFKYVSCWTDTPNSIPQWTMYSDNMTGVCIGISFDKETDVFLTEKFSLSESSEPIDM
                     VNALHPLKSGLLVTNNKYVPSLEQIRYTDDVSLITPRVVSSDDKSTTINLASNGIYKT
                     TDWSFQNEQRFSFQIFPLPIDLVLELMNANKGDLTEIINSFISVKPKEYFDLDLNPTI
                     FSNMTITFGKRCSAEDKLKVSKFLEDNKFHIPLFDSTVNIKP"
     gene            complement(1495..1764)
                     /locus_tag="AC1PIW_000185"
                     /pseudo
     CDS             complement(1495..1764)
                     /locus_tag="AC1PIW_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            1766..1864
                     /locus_tag="AC1PIW_000186"
                     /pseudo
     CDS             1766..1864
                     /locus_tag="AC1PIW_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226747.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
     gene            complement(1873..2010)
                     /locus_tag="AC1PIW_000187"
                     /pseudo
     CDS             complement(1873..2010)
                     /locus_tag="AC1PIW_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            2219..2662
                     /locus_tag="AC1PIW_000188"
     CDS             2219..2662
                     /locus_tag="AC1PIW_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent MarR family transcriptional
                     regulator"
                     /protein_id="HIP6605159.1"
                     /translation="MNRIALEIEKYLHEIVLSSENQLEILVGSCQSTVKLTNTQEHIL
                     MLIEKAAYTNTEIAKELNVSQAAITKATKSLVAQGLLVAVRDDKDARIVRFSLTEAAK
                     PIAAEHAHHHAHTLEAYEELLENYSLEEQESIARFLSELVEKIRK"
     gene            2663..3367
                     /locus_tag="AC1PIW_000189"
     CDS             2663..3367
                     /locus_tag="AC1PIW_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HIP6605160.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHIHYYLDSGEFVTLTGENGAAKTT
                     LIKATLGILKPKQGKVSIAEKSIKGKKLRMAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RQGWFRRLTAHDEEHVRISLESVGMWEHRDKRLGALSGGQKQRAVIARMFASDPDIFI
                     LDEPTTGMDAGTKDAFYQLMHHSAKKHGKSVLMITHDPDELNKYADRNIHLVRDQQSP
                     WRCFNVHEADEEVAHV"
     gene            3360..4172
                     /locus_tag="AC1PIW_000190"
     CDS             3360..4172
                     /locus_tag="AC1PIW_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HIP6605161.1"
                     /translation="MFDLSVFHYDFMQRAFLAIIAMSLFSPILGVFLILRRQSLMSDT
                     LSHVSLAGVAFGLVLGISPTLSTVLVVIVAAVFLEYLRTIYKNFMEIGTAILMSTGLA
                     ISLIVMNKSGGKSGLSLEQYLFGSIVTISQEQVIALFTIAVIVIVLTLLFLRPMYILT
                     FDEDTAFVDGLPVRAMSIAFNVVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKSF
                     KAVIFTGMGIGFFGMVMGLLTSYYAETPASASITLIFISIFLLVNVVQKFKK"
     gene            4182..5693
                     /locus_tag="AC1PIW_000191"
     CDS             4182..5693
                     /locus_tag="AC1PIW_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter solute-binding protein,
                     Zn/Mn family"
                     /protein_id="HIP6605162.1"
                     /translation="MKKVGLLFLSVSALLLGACGNSTASEDGKLNIVTTFYPVYEFTK
                     QVAGDEANVDLLVKAGTEVHGYEPSAKDIARIQEADAFVYENENMETWVHDVEKSLDT
                     TKVNVISATEGMLLLPGGEEEHEGHDHSEEGHSHAYDPHVWLSPERAITLVENIRDSL
                     VAKYPEKKDAFETNAAAYIEKLDALDAKYSETLSAAKQKYFVTQHTAFAYLALDYGLK
                     QVSITGVAADEDPTPSRLAELTEYINKYGIKYIYFEENASKSVAETLAKETGVQLDVL
                     NPLESLTDEDMKNGKDYISVMEDNLIALEKTTSQEGSEILPEEGAETAQTVYNGYFED
                     SAVKDRTLSDYAGEWQSVYPYLLDGTLDQVWDYKAKIKGGMTAEEYKTYYDTGYKTDV
                     DQINITDNTMEFVVGDKKEKFTYKYVGYKILTYKKGNRGVRFLFEATDANAGNYKYVQ
                     FSDHNIAPVKTGHFHIYFGGESQEKLLEELENWPTYYPVGLTGLEIGQEMLAH"
     gene            5796..6266
                     /locus_tag="AC1PIW_000192"
     CDS             5796..6266
                     /locus_tag="AC1PIW_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HIP6605163.1"
                     /translation="MEQTISAAEWQVMRVLWAHPGATSQEIIQALQEGFDWQATTIKT
                     LLGRLRKKNYLRMAKETSKYHYYPLISEEEHLQGQVELLLAAICSTKQGQLVEKLLDT
                     GTFSKKSLENLASKISQLQRTAPEQIACRCLAGQCTCGHHHTLRKSTSDVVDLT"
     gene            6447..6656
                     /locus_tag="AC1PIW_000193"
     CDS             6447..6656
                     /locus_tag="AC1PIW_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIP6605164.1"
                     /translation="MKQTLKLKNLSCQNCVKHVTNHLLDLDGVEEVKIQLEEQLAEVA
                     TSVAYDLERYQEVLEDTIYEVEELV"
     gene            complement(6684..7061)
                     /locus_tag="AC1PIW_000194"
     CDS             complement(6684..7061)
                     /locus_tag="AC1PIW_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIP6605165.1"
                     /translation="MTCIFCHQIEEIDILYQTEYFKVVWDIDPIQTGHLLIISKDHYD
                     TLSQVPPTIRYELSDLEVFLTEKLCQTLAIDGVTTACNDRLFNAGTHFHVHLIPRFRS
                     DGFWDQISLAQAQLDLTHFLKAL"
     gene            complement(7119..8375)
                     /gene="tyrS"
                     /locus_tag="AC1PIW_000195"
     CDS             complement(7119..8375)
                     /gene="tyrS"
                     /locus_tag="AC1PIW_000195"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIP6605166.1"
                     /translation="MNIFEELKARGLVFQTTDEEALVKALTEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVTKIQ
                     GQLSRFLDFENGDNKAEMVNNYDWFSDISFIDFLRDVGKYYTVNYMMSKDSVKKRIET
                     GISYTEFAYQIMQGYDFYELNDKHNVTLQIGGSDQWGNMTAGTELLRRKADKSGHVMT
                     VPLITDSTGKKFGKSEGNAVWLDADKTSPYEMYQFWLNVMDDDAVRFLKIFTFLSLDE
                     IAEIEKQFDAARHERLAQKILAKEVVTLVHGEEAYNQALNITEQLFAGNIKNLSAKEL
                     KQGLSNVPNYAVQAEDNLNIVELLVTSGIVNSKRQAREDVQNGAIYVNGERVQDLDYT
                     LSDSDKIDGELTVIRRGKKKYSVLTY"
     gene            8529..10952
                     /gene="pbp1b"
                     /locus_tag="AC1PIW_000196"
     CDS             8529..10952
                     /gene="pbp1b"
                     /locus_tag="AC1PIW_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP1B"
                     /protein_id="HIP6605167.1"
                     /translation="MATKSDKQDFKKKISALGLGDVVGVFLRTLKLLFNSVAVLVFLF
                     GLFGAGIGIGFVVSLFDDVKIPKTEELVAKVSEVSRISTVTYSDGSLVSEVNSDLLRV
                     PITSEEVSNYLKQAVIATEDETFETHNGVVPKAVLRAALGSVGLGSSSGGSTLTQQLI
                     KQQLVGDAPTFTRKANEIVSALALERNMTKEEILTIYLNVSPFGRNNQGRNIAGVEAA
                     AQGIFGKPAKDLTVPQAAFIAGLPQSPIVYSPYASDGTRKSDEDMVYGIERYQDVLFN
                     MYRASFLTKEEYETYKAYDIKQDFIAPAPVMADTKDYLYYKVMEEAQEVMFDYLVKRD
                     KVSKNDLKNDETKASYEELAKQELSQGGYTIKSTVDQKIYAAMQSVVANYGSVLDDGN
                     EYVETGSVLIDNATGAILGFVGGRDYATNQNNHAFDTLRSPASTIKPLLAYGIAIDQG
                     LIGSASILSDYPTNFSSGQPIMYGSGRGTGMMNLQTAIDRSVNIPAFWTYKMMRNAGV
                     DAKAYMDKMNYHIPMYDIESVPLGGGVEISVLTNTNAYQTLANGGVYNKHYIVESITA
                     SDGTVVYQHEATPVQVYSKATASIMNQLLRQVVNSGYTTTFKSRLSGLNPQAASSDFV
                     GKTGTSNEVNDVWLMLSTPRVTLGTWAGNDDNSEMYVWTGYHNNSQYVAHMVDALYNV
                     NADMFAGKFELDSSVIASSVVASTGQRAGTTQVNGRQVTVGGAMTTSYWAKNGAPVTS
                     YNFMVGGTDSDRAQAWNTIIGSQTATSSSSTQRSSSTRSSTSSSANNTNQSSETQTAT
                     SEQANNDSP"
     gene            11398..14970
                     /gene="rpoB"
                     /locus_tag="AC1PIW_000197"
     CDS             11398..14970
                     /gene="rpoB"
                     /locus_tag="AC1PIW_000197"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIP6605168.1"
                     /translation="MAGHEVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFQDFLDYG
                     LKEVFEDVLPVSNFTDTMELEFVGYELKEPKYTLEEARAHDANYSAPIYVTFRLVNKE
                     TGEIKTQEVFFGEFPIMTEMGTFIINGAERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELETDSKDIAYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTIEKDIHKNPADSRTDEALKEIYERLRPGEPKTAESSRSLLTARFFDPRRYDLAPVG
                     RYKINKKLNLRTRLLNQTLAEHVINGETGEIVLEAGTVLSRDVLEKVEAQFDELNLVE
                     YIPNDNAVLLEPVLLQKFKIVAPKDPERVVTVIGNANPAENVRTVTPADILAEMSYFL
                     NLAEGLGRVDDIDHLGNRRIRAVGELLANQVRIGLTRMERNLRERMSVQDNEVLTPQQ
                     IINIRPVTAAIKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVRD
                     VHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFIQTPYRKIDRATGTVTNEIVW
                     LTADEEDAYIVAQSTSPLDENNRFVDKIVMGRHQGNNQEFPADSADFMDVSPKQVVAV
                     ATACIPFLENDDSNRALMGANMQRQAVPLIDPKAPYVGTGMEYQAAHDSGAAIIAQHD
                     GKVVYADADKVEVRREDGSLDVYHISKFRRSNSGTAYNQRTLVKLGDIVEKGDFIADG
                     PSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDTK
                     LGPEEITREIPNVGEDALRNLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERLL
                     HAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRANGDELQSGVNMLVRVYIAQKRKI
                     KVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHLG
                     MAARNLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGVM
                     YMIKLHHMVDDKLHARSVGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVLQ
                     EILTYKSDDVTGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDNNE
                     VELRDLDEGEDDDIIHVDDLEKARAKAAADAAAAFAAEEAEGKE"
     gene            15148..18795
                     /gene="rpoC"
                     /locus_tag="AC1PIW_000198"
     CDS             15148..18795
                     /gene="rpoC"
                     /locus_tag="AC1PIW_000198"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIP6605169.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERD
                     GLFDEVIFGPTKDWECSCGKYKRIRYKGITCDRCGVEVTRAKVRRERMGHIELKAPIS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLPKEIAALKEELKTASGQKRIKAVRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMVQLDGGRFAASDLNELYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIAGNVKAAKRLIE
                     RGDDRIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEDA
                     GREGEGMVFKDADEAVMAYRNGYVHLHTRVGIATDSLDKPWKDNQKHKVMMTTVGKIL
                     FNAIMPEGLPYLQEPNNANLTEGTPDKYFLEPGSDIKAAIAELPINPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRLKDLGYYHSTLAGLTVGIADIPVIDNKAEIIEESHERVEQ
                     IKKQFRRGMITDDERYAAVTDEWRSAKEKLEKRLVEKQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMSAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLDIRSITDGKEMIEPLEERLQGRYTKKTVKHPE
                     TGAVIIGPNQLITEDIAREIVNAGVEQVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNSDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGEVIAIEEDASTRTKKVFVKGKTGEGEYVVPFTARMKVEVGDQVARGAALTEGS
                     IQPKRLLEVRDVLAVETYLLSEVQKVYRSQGVEIGDKHIEVMVRQMLRKVRVMDPGDT
                     DLLMGTLMDITDFTDANAEVVIAGGIPATARPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKRDNLLGLKENVIIGKIIPAGTGMARYRNLEPQAINEVEIIEDTVAEEL
                     AAEAELEAVTE"
     gene            18945..19310
                     /locus_tag="AC1PIW_000199"
     CDS             18945..19310
                     /locus_tag="AC1PIW_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HIP6605170.1"
                     /translation="MYQVIKMYGDFEPWWFLDGWEEDVTSRAAFDRYEDALNAFQKEW
                     VRLSEDFPMKKSKNGTMVAFWDESDQHWCEECDEYLQRYHSLMLVEARENLPAGFIKQ
                     PTQPRVRPCKLKQNNCVNE"
     gene            complement(19345..20288)
                     /locus_tag="AC1PIW_000200"
                     /pseudo
     CDS             complement(19345..20288)
                     /locus_tag="AC1PIW_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936565.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S66 family peptidase"
     gene            20374..21324
                     /gene="comGA"
                     /locus_tag="AC1PIW_000201"
     CDS             20374..21324
                     /gene="comGA"
                     /locus_tag="AC1PIW_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HIP6605171.1"
                     /translation="MIQEKARKMIEEAVADRVSDIYLVPRGECYQVYHRIMDDREFVQ
                     DLAEEEVTAIISHFKFLAGLNVGEKRRSQQGSCDYDYGSGEISLRLSTVGDYRGKESL
                     VIRLLYDNDKELKFWFEAAERLAEEIKGRGLYLFSGPVGSGKTTLMYHLARLKFPDKQ
                     ILTIEDPVEIKQEDMLQLQLNEAIGATYDNLIKLSLRHRPDLLIIGEIRDAETARAVI
                     RASLTGATVFSTVHARSISGVYARMLELGVGPEELNNALQGIAYQRLIGGGGVVDFAK
                     GNYQNHSADQWNAQIDRLFAAGHISLRQAETEKITLGSPA"
     gene            21236..22273
                     /gene="comGB"
                     /locus_tag="AC1PIW_000202"
     CDS             21236..22273
                     /gene="comGB"
                     /locus_tag="AC1PIW_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HIP6605172.1"
                     /translation="MRKLIGFLQQDISVFGRQKQKKLPLARQRKVIELFNNLFASGFH
                     LGEIVDFLKRSQLLADPYTQVLSDGLLAGKPFSSLLADLRFSDAVVTQVALAEVHGNT
                     SLSLIHIQSYLENVSKVRKKLIEVATYPIILLGFLLLIMLGLKNYLLPQLEEGNAATV
                     LINHLPTIFLSLSGLSLVAVLAGMVWFRKTNKIKVFSCLAALPFFGKLIQTYLTAYYA
                     REWGSLIGQGLDLPQIVGLMQEQQSQLFREIGQDLEQSLSNGQSFHEHIKTYAFFKRE
                     LSLIVEYGQVKSKLGSELTVYAAECWEDFFSRVNRAMQLIQPLVFLFVALMVVLIYAA
                     MLLPIYQNMEL"
     gene            22275..22556
                     /gene="comGC"
                     /locus_tag="AC1PIW_000203"
     CDS             22275..22556
                     /gene="comGC"
                     /locus_tag="AC1PIW_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HIP6605173.1"
                     /translation="MKKLVEMKVKGFTLVEMLVVLGIISLLLLLFVPNLSQQKDAIQK
                     KGDAAVVKVVESQMELYELEHDEEATVADLQAKGYITEKQAKQYATAKK"
     gene            22537..22944
                     /gene="comGD"
                     /locus_tag="AC1PIW_000204"
     CDS             22537..22944
                     /gene="comGD"
                     /locus_tag="AC1PIW_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HIP6605174.1"
                     /translation="MLRRKNKAFTLFESLLTLLVVSFLAVSLSGTVQTVFRSVQEEIF
                     LWEFEAIYKDSQKLAASSHKKVNLAIGGQEVTNGYQAVEVPRNVEVLEEKTIQFEEDG
                     GNSSLTKIRFHLSQKIVTYQLYIGSGRYKKTEE"
     gene            22916..23209
                     /gene="comGE"
                     /locus_tag="AC1PIW_000205"
     CDS             22916..23209
                     /gene="comGE"
                     /locus_tag="AC1PIW_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002282213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGE"
                     /protein_id="HIP6605175.1"
                     /translation="MVAIRKQKNKAYILLESLVALATLVTICSLILSAVDAGRRRQVW
                     ELEQQEVFNLAQMAVQTKQDDLALNGVAVQVQRTAERVAVFHEGKEVLSVVKK"
     gene            23196..23630
                     /gene="comGF"
                     /locus_tag="AC1PIW_000206"
     CDS             23196..23630
                     /gene="comGF"
                     /locus_tag="AC1PIW_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HIP6605176.1"
                     /translation="MLKSKIPAFTLLECLVVLVILSGSLLIFEGLSKLLVQEAHYQGQ
                     TVQKEWLVFSSQLRSEWDQAELVKVENGKVYVNKGEQALAFGKSWSDDFRKTNDRGQG
                     YQPMLYQVDSAAISQENQLVRIDFSFKNGEERTFIYAFKEKS"
     gene            23608..24018
                     /gene="comGG"
                     /locus_tag="AC1PIW_000207"
     CDS             23608..24018
                     /gene="comGG"
                     /locus_tag="AC1PIW_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HIP6605177.1"
                     /translation="MLLKKKVKAGILLYALLMLAVFSLLLQFYLHRQEAESRLVQVAR
                     QETTAYIMAQMVLDKVEQDLQVEKIVTGAVVNEKEANLTTGSKQKENRGRVSFQEGQA
                     RYQLKGDQLLVTVELTSDGLYTYQFPFGLSSEGG"
     gene            24075..25028
                     /locus_tag="AC1PIW_000208"
     CDS             24075..25028
                     /locus_tag="AC1PIW_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIP6605178.1"
                     /translation="MNFEKIEQAYDLLLENVQTIQNQLGTNIYDAMIEQNATYVAGQH
                     ETDLVVKNNQTLKQLDLTKEEWRRAYQFLLIKANQTEPMQYNHQFTPDSIGFILSFLV
                     DQLVPTQKVTVLEIGSGTGNLAQTILNASQKELDYLGIEVDDLLIDLSASIADVMQAD
                     ISFAQGDAVRPQILKESQVILGDLPIGYYPDDQIASRYQVASPKEHTYAHHLLMEQSL
                     KYLEKDGFAILLAPNDLLTSPQSDLLKGWLQEQANIVAMIALPPNLFGKTAMAKSIFV
                     LQKKAARSLTPFVYPLQSLQEPEAIQKFMLNFKNWKQENAI"
     gene            25078..26265
                     /locus_tag="AC1PIW_000209"
     CDS             25078..26265
                     /locus_tag="AC1PIW_000209"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HIP6605179.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVLAAGIIERIGLNDSISTV
                     KYDGKKESEVLDIPNHTVAVKILLEDLLKHNIIAAYEEITGIGHRVVAGGEYFKESAV
                     ISQQEIEQIEELSALAPLHNPAHVAAMRAFEEVLPEVLNVAVFDTAFHTTMPKHAYLY
                     PIPQKYYTDYKVRKYGAHGTSHYYVAHEAAKVLGRPIEELKLITAHIGNGVSITANYH
                     GESVDTSMGFTPLAGPMMGTRSGDIDPAIIPYLIANVEELKDAADVVNMLNKQSGLFG
                     VSGFSSDMRDIEAGIQAHNPDAVLAYNIFIDRIKKFIGQYLAVLNGADAIVFTAGMGE
                     NAPLMRNDVVEGLSWFGIELDLEKNVFGNYGDISTAESKVRVLVIPTDEELVIAREVE
                     RLK"
     regulatory      26391..26535
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            26580..27131
                     /locus_tag="AC1PIW_000210"
     CDS             26580..27131
                     /locus_tag="AC1PIW_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HIP6605180.1"
                     /translation="MEKKIPKLTVQLLAAIAMTLALVMIVENYFSIRISDTLQVQFTF
                     IPNTILGAIAGPVWAAVFAAISDPVFVLFSGQTVLFTWILIEAVSAFIYGWFFYRKPL
                     DTKNKADWLYVAGVVVLIQVVISFIMTPIALHFHFGTPWIVLYSSRLIKAVFEIPLRI
                     VVTMLVLPSLQKIPELAKLMGIK"
     gene            27187..28443
                     /locus_tag="AC1PIW_000211"
     CDS             27187..28443
                     /locus_tag="AC1PIW_000211"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIP6605181.1"
                     /translation="MNYQETRRWLSSRPASDLENGVARVNWILERLDNPQLQVPTVHF
                     VGTNGKGSTLNALQSILQSSDYTVGRFTSPSIIDFREQIVYQQEMISEEDFARIVTDL
                     QPLIEDLDQTAGLDAISEFEIVVVAMFVYFAHYQRPDILLVEAGMGGLQDATNVLAPL
                     AVVCPSIGLDHQAFLGETHAAIARHKVAVLREGVPLIYATDQPEVETVFEEHACQLQS
                     PTYAVGREILLENSRAGFAVSSPLGRVEELTLQMQGRHQEVNAALAVTTAQLIKPHFP
                     TITNETIRQGLSQAIWPGRLELIRPNLMIDGAHNNESIAVLTQLLEEKYADRDIEILF
                     AAINTKPVDQMLSQLSQFGPVSVTTFDDFRAVQLEDYPSGYERVQTYQEWVEQADLDN
                     PKKLYLITGSLYFITYVRKYILEELV"
     gene            complement(28491..29552)
                     /gene="pepA"
                     /locus_tag="AC1PIW_000212"
     CDS             complement(28491..29552)
                     /gene="pepA"
                     /locus_tag="AC1PIW_000212"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HIP6605182.1"
                     /translation="MTLFGKIKEVTELQSLPGFEGQVRNHIRQKITPHVDRIETDGLG
                     GIFGIKDTAVENAPRILVVAHMDEVGFMISQIKPDGTFRVVELGGWNPLVVSSQAFTL
                     QLQDGRTIPAISGSVPPHLSRGANAPGMPAIADIIFDAGFANYDEAWAFGVRPGDVLV
                     PKNETILTANGKNVISKAWDNRFGVLMVTELLESLSGHALPNQLIAGANVQEEVGLRG
                     AHASTTKFNPDIFLAVDCSPAGDIYGDQGKIGDGTLLRFYDPGHIMLKNMKDFLLTTA
                     EEAGVKFQYYCGKGGTDAGAAHLKNHGVPSTTIGVCARYIHSHQTLYSMDDFLEAQAF
                     LQTIVKKLDRSTVDLIKNY"
     gene            30370..30654
                     /locus_tag="AC1PIW_000213"
     CDS             30370..30654
                     /locus_tag="AC1PIW_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HIP6605183.1"
                     /translation="MKKKKTALLAGLLGAGVLTASAQFYRKIQEDRKKAQALQEVRDF
                     FADLGEIATVYVDETASTQTTLIGGVVMEAGQVFLFENTKGSIQYREEER"
     gene            30651..30971
                     /locus_tag="AC1PIW_000214"
     CDS             30651..30971
                     /locus_tag="AC1PIW_000214"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIP6605184.1"
                     /translation="MIFPTNHEEVAALIEDGKPTVFLFVTTWCGDCHYIKPHVPAIEE
                     AFPDLRFVQLDRDDFMPLAQEWAILGIPSLVVLENGKEIGRFVDKNRKTKEEIMNFLS
                     GLGR"
     gene            31016..31972
                     /locus_tag="AC1PIW_000215"
     CDS             31016..31972
                     /locus_tag="AC1PIW_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HIP6605185.1"
                     /translation="MKKSLGKILLAPVVLGLSLSLAPLVQAEIQTDVINEKWGKPTLV
                     YGGGLSDAQATEVNNLFGISDVNNVSRQVVVGTDMDQYLGTSEADTASLYSSVLVQKQ
                     DAGKGVVVDIKTPQNITLITETQYANAAITAGATDVLIDVASPIQVTGESALTGVYKA
                     LAANGETVDTARTEVAQQELETVNEVATAHTGDTNFDSSALDKAVAEIKTALADYKKS
                     NGQVASEADINTIINDVLAQNGLENVITADEVSKLVTFAKAYQETSAIDSAEVAAQLN
                     QFKQQAEQQISEAYKNLQDSGILEKIGAFFENLWKGLTGLFA"
     gene            31991..32614
                     /gene="ytpR"
                     /locus_tag="AC1PIW_000216"
     CDS             31991..32614
                     /gene="ytpR"
                     /locus_tag="AC1PIW_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HIP6605186.1"
                     /translation="MIFTYNKEHVGDVLMVIVAEDKGQAVQFERKGQVARVFLEETGK
                     TVAWNIFEASSLVELDGNGQVFLTDEQVATLNAELAKEGFTESLVNDNAPKFVVGQIV
                     ELVPHPDSDHLNICQVNVGDKTVQIVAGAPNAAQGLKTIVALPGAMMPSGSLIFPGKL
                     RGEDSFGMMCSPRELALPNAPQVRGIIELDDSAVVGEAFDPAKHWKG"
     gene            complement(32647..33426)
                     /locus_tag="AC1PIW_000217"
     CDS             complement(32647..33426)
                     /locus_tag="AC1PIW_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HIP6605187.1"
                     /translation="MKEFLMEKIQTSQPYTNAELDWLLENIGHPDPTIRDELVYASFC
                     HIFLEGLITREQAQSLLQFSQETKPFSLEGPTQKRSFTCLLYCLLLSVDNEPESIYHA
                     FLSTDDHELLFQQALDYLTIENDWSGYDEKLGWIHTAAHGADFLLATSCHDQFPAEKS
                     KEVWQTIVTCLTKQSKVFSAGEEIRLAQIPVYLLLNEKVSSQELTEWISKLDFPNQEP
                     VDYFRWLNLQHFLSSLYFQLKSQQASTEEIEQAIEGKIEPA"
     gene            33481..33876
                     /locus_tag="AC1PIW_000218"
     CDS             33481..33876
                     /locus_tag="AC1PIW_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIP6605188.1"
                     /translation="MYNKTILIGRLTAQPELTQTPTGKNLTRVTVAVNRRFKTENGER
                     EADFLNVIFWGKLAETLVSYGSKGSLISIDGELRTRKYEKDGSNHYVTEILGQSFQLL
                     ESRAQRAMRENNTGDDLADLVLEEEELPF"
BASE COUNT         9624 a         6592 c         8023 g         9814 t
ORIGIN      
        1 aaaaaaatct tacagacaag gttccaacat ccttatcctt tggaatagac gaaatcgaat
       61 gtttcatttc attatctaat ttatgccaat ctttcaaact taaagatttc gttttttcaa
      121 aagcctcttc taaaacactt gatgcagagg cttttaaaat caaagtcact gaagttgccc
      181 ctaattcatt gtcaacttct ttcaaatatg attttgttgc atgttctaaa ttttctatat
      241 cttgttcatt tattttagcg tctaactttt cctgaatcac tgcttgtatt gattctaacc
      301 aatacttcgc ttcgtaagat agacgtactc caaggtgtga gcgcattttt actcctatta
      361 ttagttttgt tgtatttgat atacacattc tatcatctat atttgcagaa atcaagtaaa
      421 aacaccaaaa gatttccaaa aataaaatat tctatttcct ttacgacaga atggacgtct
      481 taaaatattg atttaatagg ctttgagtag ttgtaagttc ttaagtatca aactcaaaat
      541 ccagtgtccg caaggacgtg ccggttcgac cccggccgcc ggtatagttt aaaagacaag
      601 gtttctaagc cttgtctttt tgttttgagg ttataaaatt ttcagttgag ttactaaaaa
      661 ttacttaatg ttttgttgta taataagtta tataagcaag aggaggagcc attatatgaa
      721 gacactttat cattatacaa cagttgaaac attgcttctg atattaaatt ataaaacatt
      781 gagattcaaa agtttacttt atgttgacga tccattagaa ccttcaactt cagactttgg
      841 taatctaggt ggatttaagt atgtgtcttg ttggactgat actcctaata gtattccaca
      901 atggacaatg tattctgata atatgacagg agtgtgtata ggaattagtt ttgacaaaga
      961 aactgatgtc tttttaacgg aaaaattttc tttatctgaa tcttctgaac caattgatat
     1021 ggtaaatgca ttacatcctt taaaatcagg cttattagtt acaaataata aatatgtgcc
     1081 ttctttggaa caaataaggt atactgatga tgtttcattg atcactcctc gagtagtttc
     1141 ttcagatgat aaatccacaa ctattaattt agcttcaaat ggtatctata agacaactga
     1201 ttggagtttt caaaatgagc aaagattttc ttttcaaatt tttccattac caatagattt
     1261 agttttggaa ttaatgaatg caaataaagg agatttgact gaaataatta atagttttat
     1321 ttctgttaaa cctaaagaat actttgattt agatttaaat ccgactatat tcagtaatat
     1381 gacaattacg tttggtaaaa gatgctctgc tgaagataag ttgaaagtat caaaattttt
     1441 agaggataat aaattccata ttcctttatt tgatagtact gtaaatataa agccttaaat
     1501 taggtcatct aatttatctg ctaaatcttt ttgcttgctt ggatacaagt gagaataggt
     1561 atcgattgaa gttgttataa aagcgtgtcc tagcctttct ttcacaacta aataatcttc
     1621 ccctcgattt atcaatagag aagcgtgtga gtgcctaaaa tcatgtatgc gtatcttttt
     1681 aagagccgca tcacgttcaa gaattttttt gtactgcttt tctattgaat tttttgtaat
     1741 cgtaatggga ctactttgaa acactgacat cgaacagaca ttagctagag ggctggctgg
     1801 actacagtta cgaattgaac aaattattct ggctcataac ttacgctatt ttgaaatgaa
     1861 ctagcaccac gggtggctta tacctttatt aacgtataca gactttgtga tatgaattgt
     1921 attgctcgaa aggtcaatgt cttgccatgt tagtgcgagt gcttctctaa gtcttagatc
     1981 tgtaaagaat aggactgtaa agagtaattt ttatttacca ttttcaacta aactctttaa
     2041 ttctaaatgt actttattgt tctcaagttc taccttattt tttcgtctca gtctagtttc
     2101 tagttctcga gtcagactag tacttacttt gagaatttag cttttaaata ttttccttgt
     2161 ttttttgttt tctatgtggt atacttaaca agttaattaa aaggttaagg ggatgcctat
     2221 gaatcgtatt gcattagaaa ttgagaagta cctgcacgag attgttttga gttcggagaa
     2281 tcaattagag attttggttg gttcttgtca aagcacggtg aagttgacga atacgcagga
     2341 gcatattttg atgcttattg aaaaggctgc atatactaat actgagattg ctaaggagtt
     2401 gaatgttagt caggctgcga tcacgaaggc tacaaagtca ttagttgctc aggggttatt
     2461 ggtggcggtt agagatgata aggatgctcg tattgttcgc tttagtttga ctgaggctgc
     2521 taagcctatt gctgcagagc atgctcatca tcacgcgcat accttggaag cttatgaaga
     2581 gcttttggaa aattatagtc ttgaagagca ggagtcaatt gctcgattct taagtgagtt
     2641 agtggagaag attagaaaat agatgagata tattactgtg gaagacttgt cgttttacta
     2701 cgacaaagaa ccggttttag aacatattca ttattatctt gatagtgggg aatttgtgac
     2761 tttgactggt gagaatggcg cagccaagac aaccctcatt aaggcgactt tggggatatt
     2821 gaagcccaaa caagggaagg tttctattgc tgagaagagt ataaagggga agaagttgag
     2881 aatggcctat ttacctcagc aaattgcaag ttttaatgct ggttttccga gcacggttta
     2941 cgaatttgta aaatcagggc gttatcctcg acaaggttgg tttcgtcgtt tgacagctca
     3001 tgatgaggaa catgttcgga ttagtttgga atcagttgga atgtgggaac atcgggataa
     3061 gcgcttaggt gctttgagtg gtgggcagaa gcagcgtgcg gtcattgcgc gtatgtttgc
     3121 ctctgatcca gatattttta ttctggatga accgacaaca gggatggatg cagggaccaa
     3181 ggatgctttt taccaactca tgcaccattc ggctaagaag catggaaagt cggttttgat
     3241 gataactcat gatcctgatg agctgaataa gtatgctgat cgaaacattc acttggttcg
     3301 tgatcagcag tctccttggc gttgtttcaa tgttcatgaa gcggatgagg aggttgccca
     3361 tgtttgatct atctgtattt cattatgact ttatgcagcg tgctttcctg gcaatcattg
     3421 cgatgagtct gttctcgccc attttggggg tcttcttgat actcaggcgg caaagtttga
     3481 tgtcggatac tcttagtcat gtttcgttgg cgggggttgc atttggtttg gtattgggga
     3541 tttcaccaac gctttcgact gttcttgtcg ttattgtggc agctgtattt ttagagtatt
     3601 tgcggacaat ttacaagaat tttatggaaa ttgggacggc tatcctcatg tcgactggtt
     3661 tggctatttc gctcattgtc atgaataaat ctggtgggaa atcagggctt agcctggaac
     3721 aatatctgtt tggttcgatt gtgacgatta gtcaggagca ggtaattgct ttgtttacga
     3781 ttgctgtgat tgtcatcgtg ctgacattgt tgtttttacg tccgatgtat attcttacct
     3841 ttgacgagga tacggcattt gtagatgggt tacctgtgcg agcaatgtcc attgccttca
     3901 atgtggtgac tggtgtcgcc attgccttaa tgattcctgc ggcaggtgct ttgctggttt
     3961 caacaattat ggttttgcct gcttctattg cattgcgact tggtaagagc tttaaagctg
     4021 ttatctttac tgggatgggt attggctttt ttggaatggt tatggggttg ctgacttctt
     4081 actatgcaga aaccccagcg agtgcaagta ttaccttgat tttcattagt attttcttac
     4141 tggtgaatgt tgttcaaaaa tttaaaaaat aaggagttaa gatgaaaaaa gttggtttat
     4201 tattcttgtc tgtctcagct ttgcttttgg gtgcttgtgg caatagcaca gcttctgaag
     4261 atgggaagtt gaatattgta acgacctttt atcctgttta tgagttcacc aagcaggtgg
     4321 caggtgatga ggcaaatgtt gatctgttgg ttaaggctgg gacagaggtt catggttatg
     4381 agccgtcagc caaagatatt gctcgtatcc aagaggcaga tgcttttgtt tatgaaaatg
     4441 aaaatatgga aacatgggtc catgatgttg aaaaatcact tgatacaaca aaggttaatg
     4501 tgattagtgc gacggagggg atgttgcttt tgcctggtgg tgaggaggaa catgaaggtc
     4561 acgaccatag tgaggagggg catagccatg cttatgaccc gcatgtatgg ttgtctcctg
     4621 agcgtgccat cacacttgta gaaaatattc gtgatagttt ggtagctaaa tacccagaaa
     4681 agaaagatgc ttttgaaaca aatgcggcag cctatattga gaaattagat gctttggatg
     4741 ctaaatattc tgaaacatta tctgctgcga aacagaaata ctttgttaca cagcatactg
     4801 catttgcata tttggctttg gactatggtt tgaaacaagt ttctattaca ggtgttgctg
     4861 cagatgagga tccaactcca tcacgtctag cagaattgac agagtatatt aataaatatg
     4921 gtattaagta tatctatttt gaagaaaatg cgtcaaaatc tgttgcagaa acacttgcca
     4981 aagagactgg ggttcagttg gacgttctta atcctcttga aagtttgacg gatgaggata
     5041 tgaaaaatgg taaggattat atttctgtta tggaagataa tctgattgcc cttgaaaaaa
     5101 caacttccca agaaggttct gaaattttgc cagaagaagg tgctgagact gcgcaaactg
     5161 tttataacgg ttattttgaa gatagtgccg taaaagatcg tactctttca gattatgcag
     5221 gtgagtggca atctgtttat ccatacttgc ttgatggtac actagatcag gtctgggatt
     5281 acaaggctaa gatcaaaggt ggtatgacag ctgaagagta caagacttat tacgatactg
     5341 gctacaaaac agatgttgat caaattaaca ttacagataa taccatggaa tttgtagtgg
     5401 gagataagaa agaaaaattc acgtataagt atgtgggtta taagattttg acctacaaaa
     5461 aaggaaatcg tggtgttcgt ttcttgtttg aagcaactga tgcaaatgct ggtaactata
     5521 agtatgttca atttagcgac cacaatatcg cccctgttaa aacaggtcac ttccatatct
     5581 attttggtgg agagagccaa gaaaaactct tggaagagtt ggaaaactgg ccgacatact
     5641 accctgttgg tttgactggt ttggaaattg gtcaggagat gctggctcac taagaataat
     5701 gagaagactg ggcaaggtgt tcagtctttc ttcttgacaa tttttgacta caaatgtaaa
     5761 ctgaagttag aaaattattg ttgggaggtg aatgtgtgga gcagacaatt tctgctgcgg
     5821 agtggcaggt catgcgggtt ctgtgggccc atcctggtgc gacttctcag gaaattattc
     5881 aggccttgca ggaaggcttt gattggcagg cgacgactat taagacactt ttagggcggc
     5941 tgcggaagaa aaactatttg agaatggcta aggaaactag caagtaccac tattatccgc
     6001 tgattagtga agaggaacat ttgcaggggc aggtggagct cttactagct gccatatgtt
     6061 ccacaaaaca ggggcaactg gttgaaaaac tgctagacac agggacattt tccaaaaaaa
     6121 gcctagaaaa tctggccagc aaaatctcgc agttacaaag gactgcacct gagcaaatcg
     6181 cttgtcggtg cttggctggg caatgtacct gcgggcatca tcatactctt cgaaaatcaa
     6241 cctcagacgt tgttgacttg acttgatgag tgtaaagctc ctgtggagct ttacagccta
     6301 ctacctgaaa atgcgaaagt agtagggttc tatctgctct tggagaaacg aagacgttcg
     6361 ctttatttcc aaccttcaaa ggttccccga acctttgaag ctagtctgaa tctgattttc
     6421 attgagtata aaaaaggaga aagaacatga agcaaacttt gaaattgaaa aacttatcgt
     6481 gccaaaactg tgtcaagcat gtgaccaatc acctcttgga cttggatggg gtggaagagg
     6541 tgaaaatcca gctggaagag cagttggcgg aggtggcaac ctctgtggcc tatgacttgg
     6601 aacggtacca agaggtgctg gaagatacta tctatgaagt ggaagagttg gtataagcga
     6661 aaaagcagcc gtcgggctgc tttttataat gctttgagaa aatgagtcag gtctagttgc
     6721 gcttgtgcaa gtgaaatttg gtcccagaag ccgtctgatc gaaagcgtgg aattaggtgg
     6781 acatggaaat gggtgccagc attaaacaag cgatcgttgc aggctgtggt cacaccgtca
     6841 attgccaatg tttgacagag tttttcagtc aaaaatactt ccaagtccga tagttcatag
     6901 cgaatagtag gtgggacttg actgagtgtg tcatagtggt ctttgctgat aatcaacaga
     6961 tgcccagttt gaatagggtc aatgtcccag accaccttga aatattctgt ctggtagaga
     7021 atatctattt cttcaatctg atggcaaaaa atacaggtca tagctgttct cctattgata
     7081 gaacaaaagg aaatcatggt ggatttcctt gaaaaatctt aataagtcaa cacagaatac
     7141 ttcttcttcc cacgacggat aacggttaac tcgccgtcaa tcttgtcact gtcagaaagg
     7201 gtatagtcca agtcttgcac acgctcacca ttgacataaa tggcaccatt ttgtacatct
     7261 tcacgagctt ggcgttttga attgacaata ccagaggtaa ctagaagttc cacgatgtta
     7321 aggttgtctt cagcctgtac agcgtagttt ggaacgttgc tgaggccttg tttgagttct
     7381 tttgcggata ggtttttgat attgccagca aacaattgct cggtgatgtt aagggcttgg
     7441 ttataggctt cttcaccgtg gaccaatgtc acgacttcct tggccaaaat cttctgagcc
     7501 aggcgttcat gacgggcagc gtcaaattgt ttctcgattt cagcaatctc atctaatgac
     7561 aagaaagtaa agattttcaa gaaacgaacg gcatcgtcgt ccatgacatt gagccagaat
     7621 tggtacattt catacggaga agtcttgtcg gcatccaacc aaacggcgtt gccttcagac
     7681 ttaccaaatt tcttacctgt tgagtcggtg ataagtggca cagtcatcac gtgaccagac
     7741 ttgtcagcct tgcggcgaag caattcagta cctgcagtca tattgcccca ctggtcagaa
     7801 ccaccgattt gtagggtcac attgtgcttg tcattgagct cgtagaagtc atagccctgc
     7861 atgatttggt aggcaaactc ggtgtaggaa atccctgttt caatccgttt ttttacagag
     7921 tctttgctca tcatgtagtt gaccgtgtag tatttaccaa catcacgaag gaagtcgata
     7981 aagctgatgt ctgagaacca gtcgtagttg ttgaccattt cagccttgtt gtcaccattt
     8041 tcaaaatcca agaagcgaga gagctgacct tggatttttg ttacccagcc gtccacggtg
     8101 tctttggttt gcaagctgcg ctccgcatcc ttgaaggaag ggtcgccaat cagaccagtt
     8161 gcaccgccaa ctagggcata aggcttgtgg cctgctaact gcaagcgacg gctagttaag
     8221 atagcaacca agtgccccaa gtgtaaacta tctgctgttg gatcgtatcc tgtgtaataa
     8281 gaaacctgtc cttctgtcaa tgcttttacc aaggcttctt cgtctgtcgt ttgaaagacc
     8341 aagccacgag cttttagttc ttcaaaaatg ttcatgtggg ctgtctcctt taggtttaat
     8401 atacaggacc ataaaccatt ttatatgatt tttggaccga acttcatttt aaaaaattcc
     8461 tccctattat accacgaaat tgcggaaaat aaacaaaatt tggtataatg gaacgataag
     8521 gagttaatat ggcgactaaa tcagataagc aagattttaa aaagaaaatc agtgccctag
     8581 gcttagggga tgtagtagga gttttccttc ggacgctgaa attattattc aattcagtag
     8641 ctgtcttggt gttcctattc ggtctttttg gtgcgggtat aggaattggt ttcgtggtta
     8701 gtctttttga tgacgttaag attcccaaaa cagaggaact ggtagcgaag gtttcagaag
     8761 ttagtcggat ttccaccgtt acctattcgg atggtagctt ggtgtcggaa gttaattcag
     8821 atctgcttcg tgtacctatt acttcggaag aggtctctaa ttatctaaaa caggctgtaa
     8881 ttgcgacaga ggatgaaaca tttgaaaccc acaatggggt tgtgcctaag gctgttttgc
     8941 gtgcggcact gggatcagtt ggtctaggtt catctagcgg tggttcaacc ttgacgcagc
     9001 aattgataaa acagcaactt gtcggagatg ctccaacctt tacgcgtaag gctaatgaga
     9061 ttgtttcggc tttggcatta gagcggaata tgaccaagga agagatttta accatttact
     9121 taaatgtttc tccatttggt cgaaataatc agggacgaaa tattgcaggg gtagaagctg
     9181 cggctcaggg aatttttggt aaaccagcga aagacttgac tgttcctcag gctgctttta
     9241 ttgcaggctt accgcaaagt ccgattgttt attctcccta tgcatcagat ggaacacgta
     9301 aatcagatga agatatggtt tacggtattg aacgctatca agatgttctc tttaatatgt
     9361 atcgggcgtc attcttgacc aaggaagaat acgaaaccta taaagcctac gatattaaac
     9421 aagattttat tgctccagct cctgtaatgg cggatacgaa agattatctc tattacaagg
     9481 tcatggaaga agcgcaagag gttatgtttg attacctggt gaaacgtgac aaggtttcta
     9541 aaaatgactt gaaaaatgat gaaacaaagg catcctatga ggagttggcc aagcaagaat
     9601 tgagccaagg tggctacacg attaagagta ccgttgatca aaaaatctat gcggcgatgc
     9661 agtctgttgt ggcaaactat ggatctgttt tggatgatgg aaacgaatat gtcgagacag
     9721 gtagtgtgtt aattgataat gccacagggg ccattttagg atttgtgggt ggtcgcgact
     9781 atgcaaccaa ccaaaataac catgcctttg atacccttcg ttcgccagcg tcaaccatca
     9841 agcctctgtt agcttacggt attgcgattg atcaaggttt gataggttct gccagcatcc
     9901 tttcagatta tccaacgaac ttttcaagtg gacaaccgat tatgtatggt tcaggacgtg
     9961 gtactggtat gatgaactta cagacggcta ttgaccgttc tgttaacatt cctgcttttt
    10021 ggacctataa gatgatgaga aatgcaggtg tagatgccaa agcgtacatg gataaaatga
    10081 attatcatat tcctatgtac gatattgaaa gtgtaccgtt gggtggtggt gttgaaattt
    10141 cagtattgac aaataccaat gcctatcaaa ctttggcaaa tggtggcgtt tataacaaac
    10201 attacattgt tgaaagcatt actgcctctg atggaacagt agtgtatcag catgaagcga
    10261 ctccggttca agtctattct aaagcaactg caagtattat gaaccagctt ttgagacagg
    10321 ttgtcaactc tggttatact acgacgttca agagtcgatt gagtggttta aacccacagg
    10381 cagcatcatc tgatttcgtc ggtaagacgg gaacaagtaa tgaagtcaat gacgtgtggc
    10441 tcatgctatc aacccctaga gtaactttag gtacctgggc tgggaatgat gataactcag
    10501 aaatgtatgt ctggacaggc taccacaata attcacaata tgtggcccat atggttgatg
    10561 cgctttacaa tgttaatgcg gatatgttcg caggaaaatt tgaattagac agtagtgtta
    10621 ttgcttctag cgtcgttgct tctacaggtc aacgtgcagg gacaacacag gtaaatgggc
    10681 gccaagtaac agttggcggt gcgatgacaa ctagttattg ggctaaaaat ggtgcaccgg
    10741 taacgagcta taacttcatg gttgggggga cagatagtga tcgagcacag gcttggaata
    10801 caattatcgg ctcccaaacg gcaacatcaa gtagctcaac ccaacgaagc agttcgactc
    10861 ggagtagcac gagttcttca gctaataaca cgaaccagtc atcagaaacg caaacagcaa
    10921 ctagtgagca agcgaataat gattcacctt gatttttcct agtacttgtg ctataatggt
    10981 ataaattatt tgtcgtgtgt cttgtttgaa atattgtcca aacaaggctt gcagcagtta
    11041 aatcaaactt tttcaaagga agatttagct gctctttttg tgtctatgag gaattttttt
    11101 gagaattgtt acctattctt aaagattcta aaaattcaga aaaaggatga atgttggtca
    11161 tcacatggct gtatataatt gaactcgacc taaaacctgt gtgaaaaagt gtatgaaata
    11221 tttactttgg tttgtcactc ccaacgtcgt aaacgtctgg tctttcttcg acattctgtc
    11281 tagaaagccc tagtcgttct gacgagggtg cgttaacgaa atcaaagatt tcggacttac
    11341 cgcttattct cactttggtt ttcacggtct cgtatcttat tacaaaggag aaaacttttg
    11401 gcaggacatg aagttcagta cggtaagcac cgtacccgtc gtagtttttc aagaatcaag
    11461 gaagttcttg atttaccaaa tttgattgaa attcaaacgg actctttcca agacttttta
    11521 gattacggtt tgaaagaagt ctttgaagat gtacttccag tttcaaactt tacggatacc
    11581 atggaattgg aattcgttgg ttatgaattg aaagagccta aatacacatt ggaagaggcg
    11641 cgtgcgcatg atgcgaacta ctcagctcca atttatgtaa ctttccgtct ggtgaataaa
    11701 gaaactggcg agatcaagac acaagaagtc ttcttcggtg agtttccaat catgactgaa
    11761 atgggtacct tcatcatcaa cggtgcagag cgtatcatcg tttctcagtt ggttcgttca
    11821 ccaggtgtct acttcaatga taaagtagat aagaatggta aagtaggtta tggatcaacg
    11881 gttattccta accgtggagc ttggctggaa ttggaaacag actcaaaaga cattgcctac
    11941 actcgtattg accgtacgcg taagattccg ttcacaacgc ttgttcgtgc gcttggtttc
    12001 tcaggcgatg atgaaatctt tgacatcttc ggtgatagcg aattggttcg caataccatc
    12061 gaaaaagata ttcataaaaa cccagcagat tctcgtacgg atgaagcgct taaggaaatc
    12121 tatgagcgcc ttcgtccagg tgaaccaaag acagctgaaa gctctcgtag tcttttgaca
    12181 gctcgtttct ttgacccacg tcgttacgac ttggcacctg ttggtcgtta taagattaat
    12241 aaaaaactca accttcgtac tcgtttgctc aaccaaacac ttgctgagca tgtgattaac
    12301 ggtgagacag gcgaaattgt cttggaagct ggtactgtct tgagccgtga cgtgcttgaa
    12361 aaagtagaag cacaattcga tgagctcaac ttggtagaat acattccaaa tgacaatgct
    12421 gttcttcttg agccagttct tttgcagaaa ttcaagattg tagctcctaa ggatccagaa
    12481 cgtgttgtga cggtgattgg taatgctaat cctgcagaaa acgtacgtac agtaacacct
    12541 gcggacatct tggctgaaat gagctacttc ctcaacttgg ctgagggtct tggtcgtgta
    12601 gatgatattg accacttggg taaccgtcgt attcgtgccg ttggtgaatt gcttgctaac
    12661 caagtacgta tcggtttgac tcgtatggaa cgtaacttgc gtgagcgtat gtctgttcaa
    12721 gacaatgaag tattgacgcc acaacaaatc atcaatatcc gtcctgttac agccgcaatc
    12781 aaagaattct ttggttcatc tcagttgtca cagttcatgg accaacacaa cccactttct
    12841 gagttgtctc acaaacgccg tttgtcagcc ttgggacctg gtggtttgac tcgtgaccgt
    12901 gctggttatg aggttcgaga cgttcactac actcactatg gtcgtatgtg tccgattgaa
    12961 acgcctgagg gaccaaacat cggtttgatc aacaacttgt cttcttatgg tcacctcaac
    13021 aagtatggct tcatccaaac accataccgc aagattgacc gtgcaactgg tacagttaca
    13081 aatgagatcg tttggttgac agccgatgaa gaagatgctt atattgtagc ccaatcaaca
    13141 tcgccacttg atgaaaacaa ccgtttcgtt gataagattg ttatgggacg tcaccaaggt
    13201 aacaaccaag agttcccagc agattcagca gatttcatgg acgtttcacc taagcaggta
    13261 gttgccgttg cgacagcatg tattcctttc ttggaaaacg atgactccaa ccgtgccctc
    13321 atgggtgcca acatgcaacg tcaggctgtt ccattgattg atccaaaagc accttacgtt
    13381 ggtactggta tggaatacca ggctgctcac gactcaggtg cggcaattat cgctcaacac
    13441 gatggtaagg ttgtttacgc agatgcggac aaggtagaag tgcgtcgtga agatggctcg
    13501 cttgatgtct accacatttc taaattccgt cgttcaaact cgggtactgc ctacaaccaa
    13561 cgtacccttg taaaattggg cgacatcgta gaaaaaggcg acttcatcgc agatggacct
    13621 tctatggaaa atggggaaat ggctcttggt caaaacccta tcgttgccta catgacgtgg
    13681 gaaggttaca acttcgagga tgcggttatc atgtctgaac gccttgtgaa agatgatgtc
    13741 tatacatctg ttcacttgga agaatacgaa tcagaaacac gtgatactaa gttaggccct
    13801 gaagaaatca ctcgcgaaat tccaaacgtt ggtgaagatg cccttcgcaa cttggacgaa
    13861 atggggatta tccgtattgg tgccgaagtt aaagagggcg acattcttgt tggtaaagtc
    13921 acaccaaaag gtgaaaaaga tctttctgct gaagagcgtc tcttgcacgc aatcttcggt
    13981 gacaagtcac gtgaagtacg tgatacctct cttcgtgtac ctcacggtgc cgatggtgtc
    14041 gttcgtgatg tgaaaatctt tactcgtgcc aacggtgatg aattgcaatc aggtgttaac
    14101 atgttggttc gtgtttacat cgctcaaaaa cgtaagatca aggtcggaga taagatggcc
    14161 ggtcgtcacg gtaacaaggg tgtcgtttca cgtattgtac ctgttgagga tatgccatat
    14221 cttccagatg gaacaccagt tgacatcatg ttgaacccac tcggggtgcc atcacgtatg
    14281 aacatcggtc aggttatgga acttcacttg ggtatggcgg ctcgcaactt gggcatccat
    14341 atcgcaacac cagttttcga tggtgcaagt tcagaagacc tctggtcaac tgttaaagaa
    14401 gcaggtatgg actcagatgc caagaccatt ctttacgatg gacgtacagg tgaaccattt
    14461 gacaaccgtg tatctgttgg tgtcatgtac atgatcaagc ttcaccacat ggttgatgat
    14521 aaacttcacg cccgctcagt cggaccatac tcactcgtta cccaacagcc gctcggaggt
    14581 aaggctcagt ttggtggtca gcgtttcggt gagatggagg tttgggccct tgaagcctac
    14641 ggtgcttcaa acgtccttca agaaatcttg acttacaagt cagatgatgt gacaggccgt
    14701 ctgaaagcct atgaagccat caccaaaggt aaaccaattc caaaaccagg tgttccagaa
    14761 tcattccgcg ttcttgtcaa agaattgcaa tcacttggtt tagatatgcg tgtccttgat
    14821 gaagacaaca acgaagtaga attgcgtgac cttgatgaag gtgaagatga tgacatcatc
    14881 cacgtagatg atcttgaaaa agcacgtgca aaagccgcag ctgacgcagc cgcagccttt
    14941 gcagcagaag aagcagaagg caaagaataa ttagaatgga tggaacaagt tggtccaaac
    15001 ctcagaactg aaagagataa gggccaacgg tcttccaccg tctgatttat agaaaaatag
    15061 cttaccagct atccattctg ctgattattg ttcaagaatt tgaggtatgg gaatccatcc
    15121 tctcacaaga aaagaaaggg attattagtg gttgacgtaa atcgatttaa aagtatgcaa
    15181 atcacgttag cttcaccaag taaggttcgt tcatggtctt acggtgaggt taaaaaacct
    15241 gaaacaatca actatcgtac acttaaacca gaacgtgatg gactttttga cgaagtcatc
    15301 ttcggtccaa caaaagactg ggagtgttca tgtggtaaat acaagcgtat ccgttataaa
    15361 gggattactt gtgaccgctg tggtgtagaa gtgactcgtg caaaagtacg tcgtgaacgt
    15421 atgggacaca ttgagttgaa agcaccaatt tcacacattt ggtatttcaa aggtattcca
    15481 agtcgtatgg gcttgacctt ggatatgagc ccacgtgcgc ttgaggaagt tatctacttc
    15541 gcagcttacg tggtgatcga tccgaaagat acaccgcttg agcacaagtc aatcatgaca
    15601 gagcgcgaat accgtgagcg tttgcgtgaa tatggttatg gttcattcgt tgccaaaatg
    15661 ggtgcagaag cgatccaaga cctcttgaaa caagtcgatc ttccaaaaga aatcgcagct
    15721 ttgaaagaag aattgaaaac agcttctggt caaaaacgca ttaaagcagt tcgccgcttg
    15781 gatgtattgg atgctttcta taaatctggt aacaagcctg agtggatgat tctcaatatc
    15841 cttccagtta ttccaccaga tttgcgtccg atggttcagt tggatggtgg ccgttttgcc
    15901 gcatctgact tgaacgaact ctaccgccgt gttatcaacc gtaacaaccg tttggctcgt
    15961 ctcttggaac ttaatgctcc aggtatcatc gtacaaaacg aaaaacggat gctccaagaa
    16021 gctgtggatg ctttgattga caacggtcgc cgtggtcgtc caattacagg accaggtagc
    16081 cgtccactta aatcactcag ccacatgctt aaaggtaagc aaggacgatt ccgtcaaaac
    16141 ttgcttggta agcgtgttga cttctcaggc cgttccgtta tcgccgttgg tccaactctt
    16201 aaaatgtacc aatgtggtgt accacgtgaa atggctatcg agctcttcaa accgtttgtc
    16261 atgcgcgaaa tcgttgcccg tgatattgct ggaaacgtaa aagctgcaaa acgtttgatt
    16321 gagcgtggtg atgatcgtat ttgggatatc ttggaagaag tgatcaaaga acacccagtt
    16381 cttttgaacc gcgcacctac ccttcaccgt ttgggtatcc aggcttttga gccagttcta
    16441 atcgacggta aagcccttcg tttgcacccg cttgtctgtg aagcctacaa cgccgacttt
    16501 gacggtgacc agatggcgat tcacgttcca ttgtcagaag aagcacaagc agaagcacgt
    16561 atccttatgc ttgcggcaga acacatcctt aaccctaaag atggtaaacc tgtcgtaaca
    16621 ccatctcagg atatggtttt gggtaactac tacttgacca tggaagatgc tggtcgtgaa
    16681 ggtgaaggta tggtcttcaa ggatgcggat gaggctgtta tggcttaccg caatggctat
    16741 gttcacttgc atactcgtgt tggtatcgca acagatagcc tcgataaacc ttggaaagac
    16801 aaccaaaaac acaaggtcat gatgacaact gtcggaaaaa tcttgttcaa cgcaattatg
    16861 ccagaaggtc ttccatactt gcaagagcca aacaatgcta acttgacaga aggaactcct
    16921 gataaatact tcttggaacc aggatcagat attaaggctg ccattgcaga attgccaatc
    16981 aacccaccat tcaagaagaa aaatcttggt aacatcatcg ctgaaatctt caagcgtttc
    17041 cgtacaactg aaacatcagc ccttcttgac cgtttgaaag acttgggtta ttatcactca
    17101 acactcgctg gtttgacagt gggtattgcc gatatcccag ttatcgacaa caaggctgaa
    17161 atcattgaag aatctcacga acgtgtagaa caaatcaaga aacaattccg tcgtggtatg
    17221 attactgacg atgagcgcta tgcagctgtt acagatgaat ggcgttcagc taaggaaaaa
    17281 ttggaaaaac gcctggttga aaaacaagat cctaagaacc caatcgttat gatgatggac
    17341 tctggtgccc gtggtaacat ctccaacttc tcccagttgg ccggtatgcg tggtctgatg
    17401 tcagctccga acggacgtat catggaattg ccaatcttgt ctaacttccg tgaaggtctt
    17461 tcagtattgg aaatgttctt ctcaactcac ggtgcccgta agggtatgac ggatacggcc
    17521 ttgaagacag ccgactcagg ttacttgact cgtcgtttgg ttgacgttgc ccaagacgtt
    17581 attatccgtg aagacgactg tggaacagac cgtggtcttg acatccgttc aatcacagat
    17641 ggcaaggaaa tgatcgagcc acttgaagag cgtttgcaag gtcgttacac taagaaaact
    17701 gttaaacatc ctgaaacggg tgccgttatc attggtccaa accaattgat tacggaagat
    17761 attgcccgtg aaattgtcaa tgcaggtgtt gaacaagtaa ccatccgtag cgtatttaca
    17821 tgtaacactc gtcacggtgt ctgccgtcat tgttatggta tcaacttggc gacaggtgat
    17881 gcggttgaag tgggtgaagc agttggtaca atcgctgccc aatcgatcgg tgagcctggt
    17941 acacagctta caatgcgtac cttccacacg ggtggtgtag cctcaaacag cgatattacg
    18001 cagggtcttc ctcgtgtcca agagatcttt gaagcccgta acccgaaagg ggaagcggtt
    18061 atcactgagg ttaaaggtga agttatcgct attgaagaag atgcttctac tcgtaccaag
    18121 aaagtctttg ttaaaggtaa aactggcgaa ggcgaatatg tggttccatt tacagcccgt
    18181 atgaaggttg aagttgggga ccaagtcgcg cgtggagcag cccttaccga aggttctatc
    18241 caaccaaaac gcttgcttga agtccgtgat gtcttggcgg ttgaaactta ccttctttct
    18301 gaagttcaaa aagtttaccg tagccagggt gtagaaatcg gcgacaagca catcgaggta
    18361 atggttcgtc aaatgcttcg taaagttcgt gtcatggatc caggtgacac agatcttctc
    18421 atgggtaccc tcatggatat cacagacttt acagatgcca atgcggaagt ggttattgcg
    18481 ggtggtattc cggcaacagc tcgtccagtt cttatgggta tcacgaaggc ttcccttgaa
    18541 accaactcat tcttgtctgc cgcatccttc caagaaacaa ctcgcgttct tacagatgca
    18601 gctattcgtg gtaaacgtga caaccttctc ggtcttaaag agaacgttat catcggtaag
    18661 attatcccag caggtacagg tatggcccgc taccgtaatc ttgaaccaca agccatcaat
    18721 gaagttgaaa tcattgaaga cacagtagca gaagagcttg ctgcagaagc agagcttgaa
    18781 gctgtaactg aataaattaa gatggcacat ctctctcttt gagggatgtg tttttgatat
    18841 tcggaaagaa accatatata tgacttgata aggaattaac aagcagaatg ttttcttaaa
    18901 tcatacaatt tcgatataat gtgtacgaaa aaggaagtaa tattatgtat caagttatta
    18961 aaatgtatgg agattttgaa ccctggtggt tcttagatgg ttgggaagag gatgttacca
    19021 gtagggctgc ttttgatcgc tatgaggatg cactgaatgc ctttcaaaaa gagtgggtac
    19081 ggctatcaga ggattttcca atgaagaaaa gtaagaatgg aacgatggtg gctttttggg
    19141 atgaatcaga ccagcattgg tgcgaggaat gcgatgaata tttgcagcgg tatcattcgc
    19201 tgatgttggt agaagcgaga gaaaatttac cggctggttt tattaagcaa cctacgcaac
    19261 ctcgtgtacg cccctgcaaa ttaaaacaaa ataattgcgt aaatgaataa taaaagagga
    19321 ttgacctttg cgggtcaatc ctttttagct gaatctgata gtaaatgctt ccgcatcaag
    19381 ttcaacatgc tcgccaatcc gaatggtaaa cagaggctgg gtatgggcaa agtccaaatc
    19441 atagagaaca gggctggttt tgagagtagg gtgcttgtcc aaaatcgcca gcaaaatctc
    19501 ctccgtcatc ttggtctctt taggaaaacg accgaagaca agagcctgtg gatttggata
    19561 ggcttgtaaa agtgcagtca agtgacgagc gataatgaga taatcgtcgt cttccgcctc
    19621 ctctaaaaat agaatatatt cgtctggtct gggagcaaac tctgttccag tcagtaggtt
    19681 gagatatttc caccaatggc aataccagag gcctggcctg gattgtagac tttccattct
    19741 gttgggtaaa aggtgcgagg ggcgtctggt aggtaccaag cgtcgctaga ccattctgga
    19801 ctcggggtca agtcaaagga gtcttgtgtc acagccttga gccaggcctg agtctggtaa
    19861 tcttgtccct cacgcatctt aaagctgcta tagaatggtc ccatgtaagt ctgtatacct
    19921 gttttggcat agatggcatt gagcagggca gttgtgtccg aataaccaca gaaaatcttt
    19981 ggattgcggg caattatgtc aaaattcaaa taaggtaata gctcattgca gttgaaacca
    20041 ccaatggttg tcaaaatggc atcgaccgac tcatccgcaa aggctgcttc caagtccgca
    20101 acacgactgg caatcggagc cgaatcgaac atatcatttt caaaataatg ttctgagaaa
    20161 gagagtttga agcccagatt ttctagtttt tccttggcgg caagattggc ctcaaaacca
    20221 ccaactcttt cgatagaaga tgacgggctg acaatgcgaa tatggtcgcc tttttttaat
    20281 tttttcatag gaagctccaa gcttttttat tattttaaca aaaaagtatg gaaaatcaag
    20341 tctccttacg aattgatagg gtgaggaggt tgtatgattc aagaaaaagc aagaaagatg
    20401 attgaagagg cggtggcgga tagggtcagt gacatttatc tggttcctcg tggtgagtgt
    20461 taccaagtct atcatcgtat catggacgat cgggaatttg tgcaagactt ggctgaggag
    20521 gaagtaacag ccatcatcag ccatttcaag tttttagcag gtttaaatgt tggcgaaaaa
    20581 cgccgtagcc agcagggttc ctgtgactat gattatggga gcggagagat ttcacttcgc
    20641 ttatcaactg tcggagatta tcgtggcaag gaaagtttgg ttatccgcct gctctatgac
    20701 aatgacaagg aactcaagtt ctggtttgag gcggccgagc ggcttgcaga agaaatcaag
    20761 ggacgagggc tctacctttt ttcgggtcca gtcggctctg gtaagaccac acttatgtac
    20821 catcttgcta ggctgaaatt cccagacaag cagattttga ccatcgagga tcctgttgaa
    20881 atcaagcagg aggacatgct gcaactccag ctcaatgaag ccatcggagc cacctacgac
    20941 aatctgatca aactgtccct ccgtcatcga ccagacttgc tcatcatcgg tgaaattcgg
    21001 gatgcggaaa ccgctcgagc agtcattcga gccagcctga caggagctac cgttttctca
    21061 acagttcatg caaggtctat ttcgggtgtc tatgctcgta tgttggaatt gggtgtcggt
    21121 cctgaggagt taaacaatgc ccttcaaggt attgcctatc aacgtttaat cgggggagga
    21181 ggtgtggtag attttgcaaa gggaaattac caaaaccatt ccgcagacca gtggaatgcg
    21241 caaattgatc ggctttttgc agcaggacat atcagtcttc ggcaggcaga aacagaaaaa
    21301 attacccttg gctcgccagc gtaaggtcat tgagcttttc aataatcttt ttgccagtgg
    21361 ttttcatctg ggggagattg ttgatttcct caaacgcagt cagcttctgg cagatcccta
    21421 tacccaggtc ttgtcagacg ggttgctggc aggcaaaccc ttttcgagtt tgctggcaga
    21481 tttgcgattt tcagatgcgg tggtcacaca ggtggctcta gcagaagttc atggtaatac
    21541 cagcctgagt ttgattcata tccagtccta tctggaaaat gtcagcaagg ttcgtaaaaa
    21601 gctgattgag gtggcgacct atccgattat attgcttggt tttctgcttt tgattatgct
    21661 tggcttgaaa aactatcttc tgccccagtt ggaggaaggc aatgcagcga cagtgctgat
    21721 taatcatcta ccgaccatct ttttatctct aagtggactt agtttggtgg cggtcttagc
    21781 tggtatggtt tggtttcgca aaaccaataa aattaaggtc ttttcctgct tggcagctct
    21841 gccatttttc ggaaaactca tccaaaccta cctgacggcc tattacgcca gggagtgggg
    21901 gagtttgatt gggcaaggct tggacctgcc gcagattgtg ggcttgatgc aagagcagca
    21961 gtcgcagctt tttcgggaga ttggccagga cctggagcag tcgctttcca atggtcagag
    22021 ttttcacgaa cacattaaga cctacgcctt ttttaagcgg gagctgagtt tgattgtcga
    22081 gtacggtcag gtcaagtcca agttgggtag cgagttgaca gtttatgcgg ctgaatgttg
    22141 ggaggatttt ttctctcggg tcaatagagc catgcagcta atccaaccgc tggtctttct
    22201 ctttgtggcc ttaatggtcg ttctcatcta cgcagccatg ttgctgccga tttatcaaaa
    22261 tatggagttg taaaatgaaa aaattagttg aaatgaaggt aaaagggttc actctggtgg
    22321 aaatgttggt cgttttaggg atcattagcc tgctcttgct cctctttgtg ccaaatttga
    22381 gccagcagaa ggatgctatt cagaagaagg gggatgcggc tgttgtcaaa gtagtggaga
    22441 gtcagatgga gctctatgaa ttggaacatg acgaggaagc aacggtggca gatttgcagg
    22501 cgaaaggtta tattactgaa aaacaagcaa aacagtatgc tacggcgaaa aaataaggct
    22561 tttaccctgt ttgaaagcct gctgaccttg ttggttgtca gttttttagc tgtttcctta
    22621 tcaggaacag tacaaacggt ctttcggtct gttcaggaag aaatttttct ctgggaattt
    22681 gaggccatct ataaagacag tcagaaatta gcagccagtt cccacaaaaa agtgaacctt
    22741 gctatcggtg gacaggaggt tacaaatggt tatcaggctg tagaggtgcc cagaaacgta
    22801 gaggtcttag aggagaaaac tattcagttt gaagaagatg gtggaaattc ttctctgacc
    22861 aagattcgtt ttcaccttag tcaaaaaata gtcacgtatc aattatatat agggagtggt
    22921 cgctataaga aaacagaaga ataaggctta tattttgcta gaaagtctgg ttgcccttgc
    22981 gactctagtg accatttgca gcttgatttt atcagctgtg gatgcaggtc gtaggcgaca
    23041 ggtttgggaa ttggaacaac aggaggtttt caacttagca cagatggctg tccagacaaa
    23101 acaagatgac ctagccttaa acggagttgc agttcaggtt caacgaactg cggaaagagt
    23161 tgcggttttc catgaaggaa aggaggtttt atcggttgtt aaaaagtaaa attcctgctt
    23221 ttacactctt ggaatgtttg gtggttctag tcattctgtc aggtagcctc ttgatttttg
    23281 aaggcttatc caaattacta gtccaggaag cgcattatca aggtcagacc gttcaaaagg
    23341 aatggttggt cttctctagc caattgcggt cggagtggga ccaggctgaa ttggtcaagg
    23401 ttgaaaatgg caaggtctat gtcaacaaag gtgaacaggc tttggccttt ggcaagtcat
    23461 ggtcggatga ttttcggaaa acaaatgata gaggccaggg ttaccaaccc atgctctatc
    23521 aggtggatag cgcagccatt tcccaagaaa accagctggt gcgcatcgac tttagcttta
    23581 aaaacggaga ggagcgaacc tttatctatg cttttaaaga aaaaagttaa ggctggcatc
    23641 ctactctatg cccttttgat gctggctgtt tttagtctct tgcttcagtt ttatctccac
    23701 cgccaagagg ctgagagtcg cttggtacag gtggcaaggc aagaaacgac tgcttatatc
    23761 atggctcaga tggtcttgga caaggtcgag caggacctgc aagtagaaaa aatagttacg
    23821 ggagcggtag tgaatgaaaa ggaagcaaat ttaaccacag gttctaagca gaaggagaat
    23881 agaggaaggg tctcctttca agagggacag gctcgttatc aacttaaagg agaccaacta
    23941 ttggttacag ttgagttaac aagtgatggt ctgtatacct atcaatttcc ttttgggcta
    24001 tcatcagaag gagggtgagt tgactcgttc tcctttttag tgcaaagtct gtgaaatttt
    24061 gataagatag gagtatgaat tttgaaaaga tcgaacaggc ttacgacctg ctattagaaa
    24121 acgtacagac tatccaaaac cagctaggta ctaatatcta tgatgccatg attgagcaaa
    24181 atgctactta cgtagctggt cagcatgaga cggatcttgt tgtcaaaaac aatcagacct
    24241 tgaaacaact agatttaacc aaggaagaat ggcgtcgtgc ctatcaattc ttgctcatca
    24301 aggccaatca gactgagccc atgcagtaca atcaccagtt cacaccagac tctatcggat
    24361 ttatcctatc ttttctagta gaccaattgg tgccgactca aaaggtgacg gttctggaaa
    24421 ttggttcggg gacaggcaat ctagctcaga ccattctcaa cgccagccag aaagaattgg
    24481 attatttggg gatcgaagtg gacgacctct tgattgattt gtcggcaagt attgcggatg
    24541 tcatgcaggc agatatttct tttgctcagg gagatgcggt acgtccgcag attttgaagg
    24601 aaagtcaagt aattttggga gatttgccta ttggctacta tccagatgac cagattgcta
    24661 gccgttatca ggtcgccagt ccaaaggaac atacctacgc ccatcatttg ctcatggaac
    24721 aatccctcaa atatctggaa aaagatggct ttgcgatttt gttggctcca aatgatttat
    24781 tgactagtcc gcaaagcgat ttgctgaaag gttggttaca ggagcaagcc aatattgttg
    24841 ccatgattgc cctgccacca aatctctttg ggaagactgc tatggccaag tctatttttg
    24901 tcttgcaaaa gaaagctgca agatcgttga cgccgtttgt ttatccctta caaagtcttc
    24961 aagaaccaga agctattcag aagttcatgc tcaatttcaa aaattggaag caagagaatg
    25021 caatttaagt gaaaatctgt tatactagtt aggaaaacgc tttaaaagag gtaagatatg
    25081 tcaaaaacaa ttgcaattaa tgcaggtagt tcaagtctaa aatggcaact ttatcaaatg
    25141 ccagaagaaa cagtactagc agcagggatt attgagcgca tcggtttgaa cgattccatc
    25201 tcaacagtta agtatgatgg gaagaaagaa agtgaagttt tagatattcc taatcatacg
    25261 gttgcggtta agattctcct agaagacttg cttaaacaca acatcatcgc tgcttacgag
    25321 gaaattactg gtatcggaca ccgtgttgtt gcaggtggag aatacttcaa ggagtcagcg
    25381 gttattagtc aacaagagat tgagcaaatt gaagagttga gtgccttggc accacttcat
    25441 aacccagcac acgtggcggc gatgcgcgca tttgaagaag tattgccaga ggtgttgaat
    25501 gtagctgttt ttgacactgc tttccataca acaatgccaa aacacgctta tctctatccg
    25561 attccacaaa aatattatac agactataaa gttcgtaagt atggtgctca tggaacgagc
    25621 cactactatg tagcgcatga ggctgcaaaa gttttggggc gtccaatcga agagttaaaa
    25681 ttgattacgg cacatatcgg aaatggtgta tccatcactg ctaactatca tggtgaatct
    25741 gtggatactt caatgggctt cacaccacta gctggtccaa tgatgggaac tcgttcaggg
    25801 gatattgatc ctgctatcat tccttatttg attgcgaatg ttgaagaact gaaagatgct
    25861 gcagacgttg ttaacatgtt gaataaacag tcaggcttat tcggtgtatc tggcttctca
    25921 agtgatatgc gtgatattga agcaggtatc caagctcaca atccagatgc agtgttggcc
    25981 tacaatattt tcattgaccg tattaagaaa tttatcggtc agtatcttgc agttttaaat
    26041 ggggcagatg ctattgtctt cacggctggt atgggtgaaa atgcaccgct tatgcgcaat
    26101 gacgtagtag aaggtttgtc ttggtttggt attgagttgg acctagaaaa aaatgtattc
    26161 ggcaactatg gtgacatttc aacggcagaa tcaaaagttc gtgtcttggt tattccgacg
    26221 gatgaagaat tggttattgc gcgtgaagtg gaacgcttga aataagaaaa actaactggt
    26281 agtcggagac tgccagtttc tcttatagtt tataccttta gaaaggtata gtttttagca
    26341 agtggacaaa atatatagtg tgtgatacaa tagactagca aagaaatttg cacagagtag
    26401 atggtttgcg tcaagtgtat gtggatggga tgttgccaca taccgaagct gatctttgct
    26461 tgcatctgat gtctcctaga gtaggaacat tggtcctggc tgagagtagc gcggtaaacc
    26521 attgcatccg ctgtcgaata cacacgacag ctccattttt tgaaaaagga gcatttttta
    26581 tggaaaagaa aattccaaaa ctaacggtgc agttgttggc tgctattgcg atgacccttg
    26641 ccttggtcat gattgtagag aactatttct ctattcggat ttctgatact ttacaggttc
    26701 agtttacctt cattcccaat actattttgg gagctattgc gggtccagtt tgggcagctg
    26761 tctttgcggc tatttcagac ccagtctttg tcttgtttag cgggcaaacg gtcctcttca
    26821 cttggatttt gattgaggcg gtatcggcat ttatctacgg ctggttcttc tatcgaaaac
    26881 cgctagacac caagaacaag gctgattggc tctatgtggc tggtgtagtt gtcttgattc
    26941 aggttgtgat ttcctttatc atgacaccga ttgccctcca tttccatttt ggaacacctt
    27001 ggattgttct gtatagcagt cgcttgatta aggcagtttt tgaaattcca ttacgcattg
    27061 tcgtgaccat gcttgtcttg ccaagtttac aaaaaatacc tgaattggcc aagttaatgg
    27121 gcattaaata aaacagtatc aagcaacagg tcatccccct gttgctactt ttgtagagag
    27181 ggaatcatga attatcaaga aactcgccgg tggctatcta gtcgtcctgc atcagattta
    27241 gaaaatggcg ttgcacgtgt caactggatt ttagaacgct tggacaatcc ccagcttcaa
    27301 gtgccgaccg tacactttgt gggcacaaat ggcaagggct cgaccctcaa cgccttacag
    27361 tctatcttgc agtcttcgga ttacaccgtc ggccgcttta catcaccgtc tatcattgat
    27421 tttcgagagc agattgtcta ccagcaggag atgatttcgg aggaagattt tgcgaggatt
    27481 gtgacagact tgcaaccctt gatcgaggac ttggaccaga cggctggact ggatgccatc
    27541 tcggagtttg agattgtagt agtggctatg tttgtctact ttgcccacta ccagcgtccc
    27601 gatattctct tggtggaggc cggcatgggt ggtttgcagg atgcgaccaa tgtccttgcc
    27661 cccttggcag tagtttgtcc gtccatcggt ttggaccatc aggcattttt gggagagacc
    27721 cacgctgcta tagcccgtca caaggtcgcc gtcttgcgtg agggggttcc gctcatctat
    27781 gcgaccgacc agccagaagt ggagacagta tttgaggagc atgcctgtca gcttcagagt
    27841 ccgacctatg cggtggggcg ggagattctt ttggaaaata gcagagcagg ctttgcagtt
    27901 tcaagtcctc tcggccgtgt ggaagagtta acactacaga tgcagggtcg tcaccaggag
    27961 gtcaatgcag ccttggcagt gacaacagct cagctcatta aacctcattt tccaacaatt
    28021 accaatgaaa ccatccgcca gggcttgtcc caagccatct ggccgggtcg cttagagttg
    28081 attaggccta atctcatgat tgacggtgcc cacaataatg aaagtatcgc cgtcctgaca
    28141 caactcttgg aagaaaagta tgctgacagg gatattgaaa tcctctttgc ggccatcaat
    28201 accaagccag tggaccagat gttgtcccag cttagccaat ttggacctgt tagcgtgacg
    28261 acctttgacg atttcagagc ggtacagtta gaagattatc cgtcaggcta tgaacgagtt
    28321 cagacctatc aggagtgggt ggagcaggcg gacttggaca atcccaaaaa actctacctg
    28381 attacaggct cgctatattt cattacctat gtgaggaagt acattttaga agaacttgta
    28441 tagaaaaaaa aaggctttgc cgggcattca acccagcaaa gtcttttgtt ttaataattt
    28501 ttaatcaaat caaccgttga gcggtctagt tttttaacga tggtctgcaa gaaggcttgg
    28561 gcctctaaga agtcatccat gctgtagaga gtttgatgtg aatggatgta gcgagcgcag
    28621 acaccgatag ttgttgatgg aacaccatga tttttcaagt gggctgcacc agcatctgtt
    28681 ccacctttac cacagtagta ttggaatttg acacctgctt cttcggcagt tgtgaggagg
    28741 aagtctttca tgttttttaa catgatgtga cctggatcat agaaacgaag cagagttccg
    28801 tcaccaattt ttccttggtc gccgtaaata tctccagctg gcgagcaatc aacagcgaga
    28861 aaaatgtctg gattgaactt ggttgtagag gcatgagcac cacgaagacc aacctcttct
    28921 tgcacattgg ccccagcaat caactgattt ggtaaagcat gaccggatag gctttctagc
    28981 aattccgtca ccataaggac accgaagcgg ttgtcccaag ccttagaaat aacatttttt
    29041 ccgttggctg ttagaattgt ttcatttttg ggaacgagga catcccccgg acgaacgccg
    29101 aacgcccaag cttcgtcata attggcaaaa ccagcatcga aaataatatc agcaatggca
    29161 ggcatgccag gagcgttagc ccctcgtgaa aggtgaggtg gtactgagcc tgagatggct
    29221 ggaatggtac gaccgtcctg caactgaaga gtgaaggctt ggctagaaac aaccagagga
    29281 ttccagccac ctagctcaac aacacggaaa gtaccgtcag gcttgatttg gctaatcata
    29341 aagccaactt catccatgtg ggcgaccact aggatacgag gagcattttc aacagccgta
    29401 tccttgatac caaaaattcc acctagccca tctgtttcaa tgcggtctac atgtggcgtg
    29461 attttctggc ggatgtggtt gcgaacctgt ccttcaaatc ccggtaagct ttgtaattcg
    29521 gtaacttctt taatttttcc aaaaagagtc attcttcttc ctccatgaca tgattactac
    29581 tattttatca cgaagagcta gacttgtctt gaaaatatag aaaaatatag aaaaatatag
    29641 aaaaatatgg atagtattga caagaatgat tgaagtttgc taggatagaa atgcccctca
    29701 aaatagagag gggagtgatt gttaagtgtg ggaagcattt atcgtcattt agtttttctt
    29761 ttattacttc gttaattcgc cttgcctaac ttcagttatg tctgcggctc attgcctcgt
    29821 actaaaagta aactaaaaga ctatatcaca gtttttcttg tatctttgtt cgtcagtttt
    29881 ctaacagttt tgtttgaaag atggctcaac aatcgtgaca atagagtggg atttatgact
    29941 tagagtttgt gtcaacatct cagcgcagtg gttgattggt agatttgttc gtgtttcaca
    30001 ctccaaatct gaccattacc tttgttgcga acattgttcg ctctatctcc aacctccaaa
    30061 ggttccccga acctttggag ctatgcgtgg gtgagactga aacggtctgg gaatagaccg
    30121 ttttagctca acaactgaaa acaaggactt gttgacgaac tcctttgttc tgtctcgctc
    30181 caaaagaaaa ccctcaacgc tggtaacgtt ggggcatttt gattgccaag tgttgacaaa
    30241 catttatcac tgtaagacca ttctatctat ttttaagtct tttgtcaagt ggtgcgagtt
    30301 tgtctcaact gtcacaagtc caatggcatt tttgggaaaa atcctgggaa atatgataaa
    30361 atagtcaaca tgaaaaagaa aaagacagcc ttgctagcag gcttattggg ggcaggtgtg
    30421 ctcaccgcta gtgctcaatt ttaccgtaaa atccaagaag accgtaaaaa agcacaggcc
    30481 ttgcaggagg tgcgtgactt tttcgctgat ttaggagaga ttgccacggt ctatgtggat
    30541 gaaacggctt ctacgcagac cactcttatc ggaggggttg tgatggaggc aggacaggta
    30601 tttttgtttg aaaataccaa aggttctatt caatacaggg aggaagaaag atgatttttc
    30661 caacaaatca tgaagaagta gctgccttga ttgaagatgg caagccaact gtttttctat
    30721 ttgtgacgac ttggtgtgga gattgccact atattaaacc gcatgtgcca gctatcgaag
    30781 aagcttttcc agatctccgt tttgtgcaac tggatcgaga tgactttatg cctttggcac
    30841 aggaatgggc tattttaggc attccaagtc tggttgtttt ggaaaatggt aaggaaatcg
    30901 gtcgttttgt cgataagaat cgcaagacca aggaagaaat catgaacttc ttatctggac
    30961 tgggtagata aattagcaga gaagctctgc catttcccaa aagaaaggaa agaacatgaa
    31021 gaaatcatta ggaaaaatcc ttctagcacc tgtggtgctg gggttgagtt tgagtcttgc
    31081 tcccttggtg caggctgaga ttcaaacaga tgttatcaat gaaaaatggg gcaagccaac
    31141 actagtctac ggtggaggtt tgtcagatgc gcaggcgaca gaggttaaca atctatttgg
    31201 tatttcggat gtcaataatg tcagtcgtca ggtggttgtt ggtacggata tggaccaata
    31261 tttaggtaca tctgaagcgg acacagcctc cctctattca tctgtcttgg tgcaaaagca
    31321 ggacgctggc aaaggggttg tggtggatat taagacgcca cagaacatca ccttgattac
    31381 cgaaacgcag tatgccaatg cggctattac agctggtgcg acagatgtct tgattgatgt
    31441 agcttctccg attcaggtaa cgggggagtc tgctttgaca ggtgtttaca aggctctcgc
    31501 agccaatggt gaaacagttg atacagcccg aactgaagta gcccaacaag agctagagac
    31561 agtcaatgaa gtggcgacgg ctcatacagg tgacactaat tttgatagtt cggccttgga
    31621 taaggcagtg gcggaaatca agactgctct tgccgactac aaaaagtcca atggtcaggt
    31681 ggcttctgag gctgacatca acaccatcat caatgatgtt ttggcccaaa acggtttgga
    31741 aaatgtcatc acagcagatg aagtttcaaa attagttacc tttgccaaag cttatcagga
    31801 aacctctgct attgattcgg ctgaggttgc tgcccagctc aaccagttca agcaacaggc
    31861 ggagcaacaa atttcagaag cctataagaa tctacaagat tcagggattt tagaaaaaat
    31921 cggtgccttc tttgaaaatc tttggaaagg cttgaccggt ctatttgcat aaaggagaaa
    31981 tgaaaaagaa atgattttta catataataa ggaacatgtc ggcgatgttc ttatggtgat
    32041 tgttgcggag gacaagggac aggctgtcca gtttgaacgc aagggtcaag tggcgcgtgt
    32101 tttcctagaa gaaacaggaa aaacagtggc ctggaatatt tttgaagcat caagtttggt
    32161 tgaacttgat ggaaatggtc aagttttctt gacagatgaa caagttgcga ccttgaatgc
    32221 tgaattggct aaggaaggtt ttactgaaag cttggttaac gacaatgctc caaagtttgt
    32281 agtcggtcag attgtggaat tggttcctca tccagacagc gaccacctca atatctgtca
    32341 ggtcaatgtt ggtgacaaaa cagtgcaaat cgtagctggt gccccaaatg ctgcccaagg
    32401 attgaaaact attgtggctc ttccgggggc aatgatgccg agcggtagcc tgattttccc
    32461 aggaaaattg cgcggtgaag atagctttgg tatgatgtgt agtccgcgtg aattggcact
    32521 gccaaacgca ccacaagtcc gtggtattat cgaattagac gactccgcag tggtcggaga
    32581 ggcctttgat ccagctaaac actggaaggg gtagaaaaag aagaattggt caggtggcca
    32641 attcttttat gctggttcaa tttttccttc aatagcctgt tcgatttctt cggtcgaggc
    32701 ctgttgtgat ttgagttgga aataaagact ggacaggaaa tgctggagat tgagccaccg
    32761 gaagtagtct actggttctt gatttggaaa gtccagcttg ctaatccatt ccgtcagctc
    32821 ttggcttgac actttttcat tcagcaggag gtagacaggg atttgtgcca gacggatttc
    32881 ctcgccagct gagaaaactt tactttgttt agttagacaa gtgacaatag tttgccagac
    32941 ttctttgctt ttttcagcgg gaaattgatc atgacagctt gtggccagaa gaaaatctgc
    33001 tccgtgggca gcggtatgga tccaaccgag tttttcatcg tagccagacc agtcattttc
    33061 aatcgtcaaa taatccaagg cctgctggaa gagtagctca tggtcgtcag tgcttaaaaa
    33121 tgcatgataa atagactctg gctcattatc gacagacagc aacagacagt aaagtaggca
    33181 agtaaaactt cgtttttgtg tggggccttc tagggaaaat ggcttggttt cttgagagaa
    33241 ttgcagtagc gactgtgctt gttctcttgt aatcaagccc tccaaaaaga tatggcaaaa
    33301 actagcatag accagttcgt cgcgtatagt agggtctgga tggccgatgt tttccaacaa
    33361 ccaatccagt tcggcattgg tatatggttg ggaggtctgt atcttttcca tcaaaaattc
    33421 tttcatgatt ttcgctcctt ttctaatcct tctacgaata gataagtagg aggatataat
    33481 atgtataata aaacaatttt aatcggtcgc ttgacggccc aacctgaact cactcaaaca
    33541 ccaactggca aaaatttgac tcgtgtaact gtcgcagtca atcgccgatt taagacagaa
    33601 aatggtgagc gtgaagcaga ttttctcaat gttattttct ggggcaaact ggcggagaca
    33661 cttgtttctt atggcagcaa gggtagtctg atttctattg atggtgagtt gcgaacgcga
    33721 aaatacgaaa aagacggcag caaccactat gtgacagaga ttctaggcca atctttccaa
    33781 ttactagaaa gccgtgctca acgtgccatg cgtgaaaata atactggtga tgacctagct
    33841 gacttggtct tggaagagga ggaattaccg ttttaaagca atatcagtcc caagaccgat
    33901 ggagtgtatt gaaaaatagg ctatactata gtcaatccta aatatttttc atatctcctt
    33961 aaaaacaccg acgaaagttg gtgtttttgg ctacttattt tctatatttc caccattcta
    34021 tcagcatgat aattagagaa agtaggagaa ctc
//
LOCUS       DBNFCK010000005        33641 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.005, whole genome shotgun sequence.
ACCESSION   DBNFCK010000005 DBNFCK010000000
VERSION     DBNFCK010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 33641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33641
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.005"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            22..1584
                     /gene="dltA"
                     /locus_tag="AC1PIW_000219"
     CDS             22..1584
                     /gene="dltA"
                     /locus_tag="AC1PIW_000219"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HIP6605189.1"
                     /translation="MSFKEESIKMISSMLERVEHFATENPDYPVYHYLGQLYSYGDLK
                     ADSDSLAAHIDSLDLPANSPVLVFGGQEYQMLAIFVALTKAGHPYIPVDSHSALDRIE
                     AILEIAEPSLVFAVADFPLETSLPVLGLSEVEAIFEKKSTYQLIHPVSEDDTYYIIFT
                     SGTTGKPKGVQISHDNLLSFTNWMIRDKEFALPERPQMLAQPSYSFDLSVMYWAPTLA
                     LGGTLFALPKELTLDFKTLFAAIQTLPFKIWTSTPSFVDMALLSREFDGEHLPDLTHF
                     YFDGEELTVKTAQKLRERFPKAVIVNAYGPTEATVALSAVAITDEMLVNYKRLPIGYT
                     KPDSPTFILDEDGQVVPNGQQGEIVVSGPAVSKGYLNNPERTEAAFFQFDGLQAYHTG
                     DLGVMTDEGLLLYGGRMDFQIKYNGYRIELEEVAQHLNKSTYIEAAVAVPRYNADHKV
                     QNLLAYVVPKAGIMEQFDRELELTKVIKQELKERMMDYMMPSKFIYRDSLPLTANGKI
                     DIKGLIAEVNAQ"
     gene            1581..2822
                     /gene="dltB"
                     /locus_tag="AC1PIW_000220"
     CDS             1581..2822
                     /gene="dltB"
                     /locus_tag="AC1PIW_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HIP6605190.1"
                     /translation="MMEFFKQLPQLEPYGNPLYFFYLVLALVPIFIGLFFKKRFPLYE
                     TGVSLAFIVLMFTGTKTMQLLSLLAYIIWQTMLIFFYKHYRQRANQSWVFYLIVCLAV
                     FPLTWVKLAPTFSQHGAIFGFLGISYLTFRSVGMVIEMRDGLLTEFSLGSFLRFLLFM
                     PTISSGPIDRYRRFTEDYKQIPERTELLNMLDQTVHYIMMGFLYKFILAYFIGHTLLE
                     PLKAVALDQGGWFNLPTIGVMYLYGFELFFDFAGYSMFAIGISNLMGIRCPINFDQPF
                     KSRDLKEFWNRWHISLSFWFRDFVFMRLVKTLLKHKVFKNRNTVSNVAYLLNMLLMGL
                     WHGVTWYYIAYGLFHALGLIINDAWIRKKKSINLARKKAGQEPLPDNRWTSFAGMFVT
                     FHTVMFSFLIFSGFLDKLWFK"
     gene            2865..3104
                     /gene="dltC"
                     /locus_tag="AC1PIW_000221"
     CDS             2865..3104
                     /gene="dltC"
                     /locus_tag="AC1PIW_000221"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HIP6605191.1"
                     /translation="MDVKETILNIIEELFMEDVSEMMDEDLFDAGVLDSMGTVELIVE
                     LESRFNITVPVSEFGREDWNTANKIISGVVELMHA"
     gene            3097..4362
                     /gene="dltD"
                     /locus_tag="AC1PIW_000222"
     CDS             3097..4362
                     /gene="dltD"
                     /locus_tag="AC1PIW_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HIP6605192.1"
                     /translation="MRKQLWQIFGPVICATFLVILTLLFFPSSSRSNFKEEKQDAVAL
                     SYISFKSKYKKNRALGDKNHRFVPFFGSSEWSRFDSMHPSVLADAYQREYTPYLLGQR
                     GAASLTQYFGMQQIGAHLKNSQAIYVVSPQWFSPKGASSKAFEQYFSSDQATHFLQTS
                     AKDDYDRYAAKRFLELKSDCSLTGMFQKVASGKELSKWDREQLNWQAYLLEKQDALFS
                     RFADSGSNLGLVETEAMKLPETFSYDRLSKVANEDGSRSTQSNDFQIDDRFYRNRLQS
                     KIKKLKQSQTKISYLQSPEYNDFQLVLNEFAKHQTDVLFVIPPVNERWMNYTGLNKDM
                     YRATVAKIKYQLTSQGFNHIADLSEQGGIDYFMQDTIHIGWQGWLELDKYVHPFLTEQ
                     QAPVHYQLKKEFLTSEWAAYKGDLNLFLK"
     gene            complement(4451..5566)
                     /locus_tag="AC1PIW_000223"
     CDS             complement(4451..5566)
                     /locus_tag="AC1PIW_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HIP6605193.1"
                     /translation="MSEITAKRVSNYELFYDLVFVLATSSLTGLLHGNHIGFKEVTTF
                     ITTNLIIMTLWMNETIYLNKYGERDFLDIFTIIPSMYLIGNMSLNFSNDFAQTALPFN
                     TFLTLSYVIILLQYYLRGRRIGFNEDIKATLQMLATYIVVFAGATLLVIFKLWTNDER
                     MLIVYIIPFLISFFFQKRMSHDPINFPHMVERCQLITIITFGEIVIAIIKNYPLLELP
                     LEGILLFFAMATLFIFYISQTYLTIDHHRKADATVLLYAHLVIVLGLNFFTVAMELFP
                     SHHNDLALPMLIVGNLIFYSGILSTSFYNQQVHQVGRRGLFIYALILLIGNVALLLDG
                     HSNILLFVILNLLSHAMVAYHVIRFGKANHSLLGEDM"
     gene            complement(5639..6595)
                     /locus_tag="AC1PIW_000224"
     CDS             complement(5639..6595)
                     /locus_tag="AC1PIW_000224"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIP6605194.1"
                     /translation="MGLLQDGKWVDQWYDTKSTGGKFIRSVTQFRNWITPDGQAGPTG
                     EGGFKAESGRYHLYISLACPWASRTLIMRKLKGLEDHISLSVVHPLMLENGWTFEDGP
                     GVIKDTLFNSDYLYQVYLKADPHYSGRVTVPVLWDKKTNTIVSNESAEIMRMFNSAFN
                     DITGNYDDYYPANLQAEIDAMNDFVYPNINNGVYKAGFSTSQAVYEKEVKNLFAALDK
                     LEEHLADKNYLVGNQLTEADIRLFTTLVRFDPVYFGHFKCNIKALVDYPNLWNYTKRI
                     YNHAGIAETVDFDHIKQHYYGSHKTINPTGIVPVGPDLDWTL"
     gene            6734..7450
                     /locus_tag="AC1PIW_000225"
     CDS             6734..7450
                     /locus_tag="AC1PIW_000225"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002282339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIP6605195.1"
                     /translation="MRLDKCLEKAKVGSRKQVKKLFKAQQIKINGQAAQSLSQIVDPE
                     LQTIQVSGKKVALEGSAYYLLHKPAGVVSAVTDQEHQTVIDLISPQDSREGLYPVGRL
                     DRDTEGLVLITNNGPLGYRMLHPSHHVDKVYYVEVNGCLAEDASKFFASGVTFLDGTR
                     CQPADLTVLEASLDHSRATIKLAEGKFHQVKKMFLAYGVKVTYLKRISFGGFELGDLE
                     RGTYRQLTPNEMEHLFTYFD"
     gene            7464..7943
                     /locus_tag="AC1PIW_000226"
     CDS             7464..7943
                     /locus_tag="AC1PIW_000226"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HIP6605196.1"
                     /translation="MSIYDFTVQKQDGTDQSMAEYQGQVLLIVNTAPGCGLAPQYKEL
                     QELYDSYKDNGFVVLDFPCNQFLNQAPGSAEDINQICSLNYGTTFPRFAKIAVNGSEA
                     SPLYRYLKKEKSTLLGGRIEWNFTKFLVDRQGRVVKRYLPTTSPLKLKEDIELYLEK"
     gene            complement(7962..8963)
                     /locus_tag="AC1PIW_000227"
     CDS             complement(7962..8963)
                     /locus_tag="AC1PIW_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIP6605197.1"
                     /translation="MIRHHNYLKIFTFLLILLFIIFLLSLSIGYSNSSFSDFIDIMSG
                     RASSSTLLIIGRIRLPRIIASIIGGASLALAGLLLQTLTRNPLADSGILGINAGAGLV
                     VALFVGLSSKISPVLLSLMPLFAMMGGGLTIFLVYWIARKKLYGIHPARLIITGVGIS
                     SMLSGIMVSIISQLDDFKMEYIVQWLSGRVNGGDWKTIAIYTPLLLLVWMATFSRSRS
                     LNIMNLNDQTAMALGLHLQRERRITLILATALASLSVILVGNITFVGLIAGHIVRKWL
                     GNDHRVIIPATMIIGSFILLLADTIGRVLLVGTGIPTGIIVSLIGAPYFLWLLKQEG"
     gene            complement(8960..9985)
                     /locus_tag="AC1PIW_000228"
     CDS             complement(8960..9985)
                     /locus_tag="AC1PIW_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIP6605198.1"
                     /translation="MNKLVSSRYPKTKPKNIWLVFFIICLSFIAGVYLSLRFGAISYS
                     HQQLIETLKHPLTNSSAQDVIIDLRLPRMVAAILVGAAMAQAGAMMQGITRNPIADPG
                     LLGINAGAGLALIVAYALFGGLHYSQILLICLLGSCLAAGLVFGLAYQVQKGYNQLRL
                     ILSGAMVASLFSAIGQAITIYFDLSTAVIGWQAGGLVQVNWKMLAIIAPLIIIGLILA
                     QLFSHQLTILSLNETVAKNLGQRTLLMTLVLLGIVLLLSASAVALVGSLSFIGLIIPH
                     FIRMFTGKNYKLLLPLTAFAGASFLIWVDLVCRSINPPVETPISAVISIIGLPCFLWL
                     IRKEKHF"
     gene            complement(9999..10928)
                     /locus_tag="AC1PIW_000229"
     CDS             complement(9999..10928)
                     /locus_tag="AC1PIW_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIP6605199.1"
                     /translation="MKKFLAIFSLFVGLVFLTACSTSSSSTDVELSSMPKIEGITYHG
                     DIPKNPKKVVNFAYSYTGYLLQLGIDVSSYSLDLEKNSPVFGDKLKDVPQLTTADTEA
                     IAAQKPDVILVFSGDDNLETLKEIAPVIEITYGKSDYLKMLTDIGQIFGKEKEAQAWL
                     DQWDKKVTTAKEELKGLIDTSSTFTVMDFFDKNIFLYGNNFGRGGELIYRALGFAAPA
                     KVQEDIINKEGWFGVSQEALPEYVGDYVLVNVNDKTKEAAASLKESDIWKNLPAVKNG
                     HVIEIDYNLFYFSDPMSLDLQIDAFVSEIQKLQ"
     gene            complement(10941..11726)
                     /locus_tag="AC1PIW_000230"
     CDS             complement(10941..11726)
                     /locus_tag="AC1PIW_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6605200.1"
                     /translation="MSSIQAENIQVSYDNRIIIDELSTSIPKGSITTIIGANGCGKST
                     LLKALTRIIPVQEGAIYLDGQAISQLPTKEVAKKLALLPQVLEATEGISVYELVSYGR
                     FPHQNGLGHLTDQDREKINWALEVTRTAPYARLPVDDLSGGQRQRVWIAMALAQDTDT
                     IFLDEPTTYLDLNHQLEVLELLKELNQSRQKTIVMVLHDVNLSARFSDYMIAMKEGDI
                     RYHGSVSNMMTTEILRDIFSIEPQLMQVAGQDYPILLTYDLKK"
     gene            complement(11878..13323)
                     /locus_tag="AC1PIW_000231"
     CDS             complement(11878..13323)
                     /locus_tag="AC1PIW_000231"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013990079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HIP6605201.1"
                     /translation="MITIERVLEILKADANFRHIKQNDQTDSTWTDVQFDALSYDSRT
                     VSPMTLFFAKGLAFKKEFLEKAIEAGLAFYVSEINYEVGIPAIIVHDIKQAMSLIAME
                     FHGHPQKQLKLLAFTGTKGKTTAAYFAFNILKQSKKPAMLSTMNTTLDGKTFFKSTLT
                     TPESLDLFAMMAEAVKNGMTHLIMEVSSQAYLVKRVYGLTFDVGVFLNISPDHIGPIE
                     HPTFEDYFYHKRLLMDNSRAVIVNAGMDHFEVVKEQVSSKDHDFYGPTSENQISQSAG
                     FDFTATGKLAGHYDIQLIGGFNQENAIAAGLACLRLGASLEDIHTGIAQTNVPGRMEV
                     LTQQNGAKVFVDYAHNGDSVKKLIDVVLEHQTGKVFLILGAPGNKGESRRKDFGLLLN
                     DYPQIEVILTADDPNREDPAAIAEQIRAHMTRTSNFILDREEAIRTAMSQTSSPKDAV
                     IIAGKGADAYQIVNGEKAAYDGDLEVAKQYL"
     gene            13470..14219
                     /locus_tag="AC1PIW_000232"
     CDS             13470..14219
                     /locus_tag="AC1PIW_000232"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIP6605202.1"
                     /translation="MFYSYLRTLLTFLLWAINGNIHYHDREKILPQEENYILIAPHKT
                     FWDPVFLGYAAAPKQFIFMAKKELFKDRGFGWWISKCGAFPIDRDNPGMAAIKYPVNM
                     LKKSDRSLVMFPSGSRHSSELKGGVAVIAKSAKVKLMPATYVGPMTIKGLLAGERIDV
                     AFGNPIDVSDIKRMDDAGTAEVTSRIEAEFKRLDDHAASFQTKKKPNILTYIYRIPVL
                     LLVALILGLTYAFSYIASFFWQPSTQLDKNK"
     gene            14282..14944
                     /locus_tag="AC1PIW_000233"
     CDS             14282..14944
                     /locus_tag="AC1PIW_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIP6605203.1"
                     /translation="MDTIKTYIEMLKEYKWQIALPAVAGLLMATFLIFSQPAKSDQTG
                     LTDFPQTEQASSSQEQTEETSTEESEELSQLTVDVKGAVEKPGLYTLEAGARVNDAVE
                     AAGGLTSQADPKSINLAQKLSDEAVVYVASKDENISVVASTTASSAMSPEEKSTSLVN
                     LNTATEADLQTISGIGAKRAADIIAYREANGGFKSVNDLNNVSGIGDKTMESIRPYVT
                     VE"
     gene            14928..17165
                     /locus_tag="AC1PIW_000234"
     CDS             14928..17165
                     /locus_tag="AC1PIW_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HIP6605204.1"
                     /translation="MSRLSKLPCQPVHLAVLAVAAYFAVHSFSLLTMCLLSLVLAVFG
                     VRQGKTVFLKTLPLLTLCGLFFGCQKVQWERAAQSAPEQVTTVQVIPDTIDINGDSLS
                     FRGRADGQNYQVFYKLTSQEEQTYFQNLADLVQLEVEAEVSLPAGQRNFKGFDYQAYL
                     KTQGIYRTVKLTAIKKIVPVQSWNVFDWLSTWRRQALVYVKTNFPAPMSHYMTGLLFG
                     ELDSDFDQMSDLYSSLGIIHLFALSGMQVGFFIDKFRWILLRLGLTKETVDKLQIPFS
                     LVYAGLTGFSVSVVRSLVQKILGNLGLRKLDNFAVTVFICLLILPRFLLTAGGVLTFT
                     YAFLLTVFDFEDLGQVKKAAVESLSISLGILPVLMTYFYAFQPLSILLTFVFSFVFDV
                     LLLPGLSVIFLLSPVVKMTWVNGFFVFMEKIIVWVADLGLRPWILGKPSELVLLLLLV
                     SLFLLYDFHRRKKWLLGLSLVLALLFFITKHPLENEVTVLDIGQGDSIFLRDMRGRTV
                     LIDVGGRVDFAAKEAWQERSRQANAERTLIPYLHSRGVDRIDSLVLTHTDTDHVGDVL
                     EVAKQVQIGRIVVSPGSLTVPDFVATLKKINVPVHVVKVGDRLPIFDSYLEVLYPDGT
                     GDGGNNDSIVLYGRLLETNFLFTGDLEQGELDLIETYPNLPVDVLKAGHHGSKGSSYP
                     EFLDHIGAKIALVSAGENNRYKHPHQETLERFDSRNIQVYRTDQQGAIRFRGWKEWKI
                     ETVRR"
     gene            17182..17307
                     /locus_tag="AC1PIW_000235"
                     /pseudo
     CDS             17182..17307
                     /locus_tag="AC1PIW_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
     gene            17490..18146
                     /locus_tag="AC1PIW_000236"
     CDS             17490..18146
                     /locus_tag="AC1PIW_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIP6605205.1"
                     /translation="MSVKDFMTRKVVYISPDTTVAHAADIMREQDLHRLPVIENDKLV
                     GLVTEGTIAEASPSKATSLSIYEMNYLLNKTKVKDVMIKNVITVSGYASLEDAAYLMY
                     KNKVGILPVVDNGQLYGVITDRDIFAAFLHVSGYGEEGVRARFLVENKAGELEKIIRL
                     VADKDYNIVSTVQLATKSGKVVIEVQIDGKVDVEEVRTLFEGAGIQVDSLVPTVAKNI
                     "
     gene            18240..19067
                     /locus_tag="AC1PIW_000237"
     CDS             18240..19067
                     /locus_tag="AC1PIW_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605206.1"
                     /translation="MGHKYKKYKKYGKNNSGLVALLVLLAIGFVMSFWYILLPTGLLI
                     GYFYKREAIQRFLNRDSIKRLQVLALSIRSGYEKHQELLVENGEDKVVLQLRNRLLGQ
                     LFELDQLYQKFGKYLNGYESKEVVDTLQLKYHLKLPEAKTEKNTDTQVSTGGNAVMDE
                     QGMIADLAPEILETYCNIQRDNLVILEKLEKATDKREELTAIHEANMNRFNDILQGYL
                     KIKKSPKDYFNAEERLVQAKSAMEMFDKDLDDTIKQFNEADMKDFEVSLRMMKREEE"
     gene            19073..20344
                     /locus_tag="AC1PIW_000238"
     CDS             19073..20344
                     /locus_tag="AC1PIW_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HIP6605207.1"
                     /translation="MSGFNFDIDQIANNSLSTKDKTTELIQATPTGDNSKVSFLAQLT
                     PEQQTGIRAKAPQLVDNFLANQNVLLDFGKEAVEEVNATVNHILSEQKKIEIPQVDEL
                     LANTNRELNGFVAKYKDISTTAELEKKPGFFQHLFKQTKNDLQEFYFDSQTIEKKMDS
                     MAASVVKQEEVLARNIVSAELLIENNTKSIENLVGVIAFIEATGQEAAQRAKAEQERL
                     AGLQPTTVDYQVASEKLARVTEVANILEQQHSEYMSRLYVAWTTTPQMRNLVKVSSDM
                     KQRLGMLRRNTIPTMKLSIAQLGILQQSIKSSQTADAIVNANNAALQMLADTSKEAIP
                     MMERTAQNPTLSVASVTKLAESLVAQNNGIIAAIDEGRQKRRELEAAIVRSAETINDS
                     VKLRDQKIIAALLEQGKEAQSEAEQPLETNQ"
     gene            complement(20381..21247)
                     /locus_tag="AC1PIW_000239"
     CDS             complement(20381..21247)
                     /locus_tag="AC1PIW_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIP6605208.1"
                     /translation="MVRLKNILLILLGAGLFAFGLNYLIMPNRLFEGGATGLTLIIYY
                     LFHIQPWIMNIVINIPLFILGWKILGKKTLYLSILGTFSVTFWLAIFEKIHFSINLQQ
                     DLILVSILGGILMGLGLGTIFRAGGTTGGSDIIARIGHKFLPYSIGQIILAVDILILT
                     LIVIVFKDLRTVLYTLMMVAIASKVIDFVTEGGYGSKGVMIVSQKSDQLAQAIDCEIE
                     RGVTFIKAQGFYSKADVNMIYSVIYKSQLQEMKELIHRIDPHAFITITDAHEVLGEGF
                     TLDRDKKPIERH"
     gene            21422..22060
                     /gene="tmk"
                     /locus_tag="AC1PIW_000240"
     CDS             21422..22060
                     /gene="tmk"
                     /locus_tag="AC1PIW_000240"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIP6605209.1"
                     /translation="MRNGFFITFEGPDGAGKTTVLQQLLPALQELGPEVVTTREPGGV
                     AIAEEIRNIILNPANTAMDDKTELLLFIAARRQHLKEKILPPLAQGKLLLIDRFIDSS
                     IAYQGFGRGLDIGDIHWLNQFATDGLKPDLTLYFDIDTEEGLARIARNADRDVDRLDM
                     EKADMHRRVRQGYLSILEQEPERFVKIDASQPLEAVVANALAVIKKRFAERT"
     gene            22057..22941
                     /locus_tag="AC1PIW_000241"
     CDS             22057..22941
                     /locus_tag="AC1PIW_000241"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HIP6605210.1"
                     /translation="MKIEELRNLQPYVFQRFVTILEQRRLAHAYLFSGDFASFDIAIF
                     LSQSLFCQEKEGILPCQKCRSCRLIEANEFSDLTVIAPQGNVIKTDTIRELVKNFSQS
                     GFESNKQVFIIRDAEKMHANAANSLLKVIEEPQSAIHIFLLTNQEEAVLPTIKSRTQI
                     IGFPKNIPAMERMLEEEGLLKTQANLLAQLVSSQEEAKKLAENKNFLDLMGQAKKFTD
                     LLLVEPTRAYLQVGILVSLAVEKAEQGRLFDLLSLILSEKMMESANVREKMDAVLKAK
                     KMWQANVSLQNSLEYITL"
     gene            22961..23278
                     /gene="yabA"
                     /locus_tag="AC1PIW_000242"
     CDS             22961..23278
                     /gene="yabA"
                     /locus_tag="AC1PIW_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HIP6605211.1"
                     /translation="MDKKEIFDALDDFSQNLLTTLAEVDAIKKHLQGVIDENTTLRLE
                     NSKLRERLEKEDKTGHKSSNFGKENLENIYEDGFHICTFSYGQRRDNDEPCMFCVELL
                     NRD"
     gene            23280..24143
                     /gene="rsmI"
                     /locus_tag="AC1PIW_000243"
     CDS             23280..24143
                     /gene="rsmI"
                     /locus_tag="AC1PIW_000243"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIP6605212.1"
                     /translation="MKVQKSFKGQTSFGTLYLVPTPIGNLQDMTFRAVQILKEVDIIA
                     AEDTRNTGLLLKHFEVETKQISFHEHNAHEKIPVLIDWLKSGQSIAQVSDAGLPSISD
                     PGHDLVGAAIAEDIPVVALPGASAGITALIASGLAPQPHIFYGFLPRKSGQQKEFFQE
                     KKTYPETQIFYESPYRVADTLENMLTVYGDRQVTVVRELTKLYEEYQRGSISELLEYL
                     EENPLKGECLIIVEGAGEEVYPSADEVDLKAEVEREIASGIKPNQAIKEVAKRYQLKK
                     QEVYDIYHGLG"
     gene            24613..25704
                     /gene="serC"
                     /locus_tag="AC1PIW_000244"
     CDS             24613..25704
                     /gene="serC"
                     /locus_tag="AC1PIW_000244"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HIP6605213.1"
                     /translation="MTIYNFSAGPAVLPKPVLERAQAEFLDYNGSGMSVLEMSHRSKD
                     FDDIIKGAEATLRELMAIPDNYKVIFLQGGASLEFTMIPLNFAQGKKAYYLVGGSWGK
                     KAYTEAVKLSKTIAFEPILLGSTEDITYAELPTFDKNDIDPTAAYVHLTTNNTIEGTA
                     VYDIPDTNGVPVIADMSSNILAARYNVEDFAMIYAGAQKNIGPAGVTVVIVREDFLND
                     QPMLSSMLDYRIQAENDSLYNTPPAYSIYISKLVFEWVKEIGGVDEMEKINREKSGLL
                     YDYIDQSNFYTNPVRKKEERSVANIPFVSPSEELDAKFVKEATAAGFKNIKGHRSVGG
                     MRASLYNAFPRQGVVELIEFMKKFAVENA"
     gene            25704..26261
                     /locus_tag="AC1PIW_000245"
     CDS             25704..26261
                     /locus_tag="AC1PIW_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6605214.1"
                     /translation="MEIRLAHPNEMEAIVNILEDARAFLATSGINQWQGEYPNKENIF
                     DDILSGRGYVGLVDGKVAAYAAVHRGQEAEYEAIYEGKWQHNHPVYTTFHRIAAADEF
                     RGQGIIQTFLQGLIEGQKGPDFRCDTHEQNKTIQHILKKLGYVYCGKVPIDGERLAYQ
                     KIKQKSERSLYQEISEDDRWLLGNN"
     gene            26315..27496
                     /locus_tag="AC1PIW_000246"
     CDS             26315..27496
                     /locus_tag="AC1PIW_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HIP6605215.1"
                     /translation="MVFSVRTFNNINQIGLKELGNKFQIDGDHAANPDAFIIRSENLH
                     GFDFPENLKAIARAGAGTNNIPIEEATEKGIVVFNTPGANANAVKEAVIASILLSARD
                     YIGATAWANTLSGDDVPKQVEAGKKQFAGTEISGKTLGVIGLGAIGARIANDARRLGM
                     NVLGYDPYVSVETAWTISSHVKRVDDLKEIFTNADYITVHVPLTDKTRGLFNADSFCQ
                     MRKGTTLINFARGELVNNADLFEAIEAGVIKNYITDFGTEEVLNKDNIIVFPHVGGST
                     EEAELNCAIAAGQTIRRFMETGEIINSVNFPNVKQFLDAPYRITLINKNIPNMVATIT
                     TAVSELGINIDNIINKSKGDYAYTLLDLDEADKVKIEQLVAKFEATDAIVKVRVIQNK
                     N"
     gene            27499..28005
                     /locus_tag="AC1PIW_000247"
     CDS             27499..28005
                     /locus_tag="AC1PIW_000247"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIP6605216.1"
                     /translation="MLYKQVYHSPLGPMSLIASDKGLRGAWFEGQKYFERGLNEKAIL
                     ASHPILDQTARLLDQYFSGDQVDFSNLPLEAVGTNFQEHVWQLLKIIPMGQTTTYGQL
                     AQQLGLRSGQAVGGAVGRNPYSIIVPCHRVLNQKGQLTGYAGGLDKKIWLLRHENPEF
                     EVKDDFIL"
     gene            27989..28342
                     /locus_tag="AC1PIW_000248"
     CDS             27989..28342
                     /locus_tag="AC1PIW_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HIP6605217.1"
                     /translation="MILFYEYPKCSTCRAAKAELKSLGLEFEAIDIKSTPPSAEEIKA
                     WMEATGLELKKYFNTSGNSYRELGLKDKFDSLSLEQALNLLANDGMLIKRPLLIQDGK
                     ILQIGYRTKYENLGL"
     gene            complement(28359..29258)
                     /locus_tag="AC1PIW_000249"
     CDS             complement(28359..29258)
                     /locus_tag="AC1PIW_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HIP6605218.1"
                     /translation="MDHKIIERKSLTVSSVVNGLSGLAGIAVYIMTDLNALLLDGVFS
                     LIAFISSLVAVYISKNSHRKTETFPQGLYFLEPLYAIMKSLATLLLLVFAVLETSATA
                     FAYFVHGIGHQMTTGPVLPYTITMFFVCMGLYLYNRHMSAKVNHLSTIILAEAKGNLV
                     DGLISGAIGIAILLLYLVPINSWFGFLHYTGDFFLTLALAIISFKEPWNLLVASFKEL
                     ANGTIHFPEIHDSIYQLLEPYLKEDSNDVEIHIFKQGMKIRVKINLHNVHHEIIQKLL
                     EDKPQMLYLLKKHHEYISIEYAL"
     gene            complement(29445..30272)
                     /locus_tag="AC1PIW_000250"
     CDS             complement(29445..30272)
                     /locus_tag="AC1PIW_000250"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIP6605219.1"
                     /translation="MKLISWNIDSLNAALTAESPRALLSRAVIDTLVTEDADIIAIQE
                     TKLSDKGPTKKHLEILESYFPGYANTWRSSVEPARKGYAGTLFLYKNHLTPTITFPEI
                     GAPTTMDSEGRIITLEFDDFYVTQVYTPNAGDGLKRLADRQIWDVQYADYLAKLDSHK
                     PVLATGDYNVAHKEIDLANPASNRQSPGFTDEERQGFTNLLAKGFTDTFRHLHGDVLN
                     AYTWWAQRSRTSKINNTGWRIDYWLVSDRIADKVSKSDMIDSGARQDHTPIVMEISF"
     gene            30428..30607
                     /locus_tag="AC1PIW_000251"
     CDS             30428..30607
                     /locus_tag="AC1PIW_000251"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605220.1"
                     /translation="MKRKLFVFAWLALVLEMLIYYWYQLPALNILSVDFWIFFLQSVG
                     LVWLILSTFSSKGAF"
     gene            30611..32089
                     /locus_tag="AC1PIW_000252"
     CDS             30611..32089
                     /locus_tag="AC1PIW_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605221.1"
                     /translation="MTKISGRQRQVDTYQIKTSQLPAYLKWLGRIWVLVVLGLLGLGL
                     INSRVFRAKDYAAVISVKDADFKADFPETDISKLALLDRASAEKIGDTYLGTIDKVSQ
                     FGISDDYRQITIGQQPFRVSPLEYKNFWKWLSNHQDGIGYYVKVNQTTGKAELAKLSK
                     AMHYSDSEFLLNDTLRHLRLQYPTTIFGKPSFEVDDQGNPYYIATIYEPKFGLSSNDP
                     IGAIVLDAVTGESKKYGLEDIPEWVDRVYSANNVISRVDDHYTYQNGFWNTIFSQTGV
                     KNTTDSYNYISIGSDIYLYTGITSATADSSNLGFILVNMRTREITNYKLASATETAAQ
                     KSAEGEVQEKGYKATNPSLVKLADTAYYLVSLKDDAGLVKSYALVDAEDYQQVTVNND
                     IALLISQVTGTDASSLSGLTVTENGEVGEQTEVISGKVEALASQIIGGATIYYIESEG
                     KIYKVKATEDSTDRLPFIKVGDHFSGQLDKKNYLKNFKVNPE"
     gene            32249..33637
                     /locus_tag="AC1PIW_000253"
     CDS             32249..33637
                     /locus_tag="AC1PIW_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIP6605222.1"
                     /translation="MSLFRKKQAVGRHSQMRRHLGLVDLIFLGIGSMVGTGIFTVTGL
                     AAAQYAGPALIISIVIAAISVGLTALFYAEFASRIPTNGGAYGYLYSVFGEFPAWIAG
                     WLTIMEFLTAVSSVASGWGAYLKGLLANFGIAMPTALNGTFNPAAGTYVDLLPVLVLI
                     FVVGVVLLNSKAALRFNSALVVLKFSALALFILVGLFFIKPENWSNFSPFGFGAIYGG
                     QAGIMAGASLMFFAFLGFESISLAIDEVKKPEKNVPKGIVLSLSIVTILYIVVTLVLT
                     GMVHYTKLNVADAVAFALREVGLDWAASYISIVAILTLITVCISMTYALSRMVYSISR
                     DGLLPKSLSRLTQTSKVPKNATILVGIFAAICAGIFPLASIASFLNICTLAYLIMLAL
                     GIIRLRQVEGLPKDGQFKTPLVPLLPILSIIICLSFMFQYSLDTWLAFGVSLVIGILI
                     YFCYGYRHSEAK"
BASE COUNT         9859 a         6295 c         7612 g         9875 t
ORIGIN      
        1 gacagggagg atttatctat aatgagtttt aaagaggaga gtataaaaat gattagcagt
       61 atgttagagc gggtagagca ttttgcgaca gaaaatcctg actaccctgt ttaccattat
      121 ctaggacagt tgtatagtta tggggattta aaggcagatt cagattcttt agcagcccat
      181 attgatagtt tggatttacc agcaaactct cctgtccttg tttttggcgg acaggagtac
      241 cagatgttgg caatctttgt agccttaacc aaggctggcc atccgtatat ccctgtagat
      301 agtcattctg ctttggatcg gattgaagct attctggaaa tagcagagcc gagtttggtg
      361 tttgcggtag cagactttcc gctagagaca agtttgccag tgcttggttt gtctgaagtt
      421 gaagccattt ttgagaagaa gtcgacttat cagttgatac atcctgtaag cgaagatgat
      481 acctactata ttatcttcac ttctgggaca acgggtaaac caaaaggagt gcaaatttcg
      541 catgacaatt tgctcagctt tactaactgg atgataaggg ataaggagtt tgcgctacca
      601 gagcgaccgc aaatgttagc tcaaccttcc tattcgtttg acttgtcggt tatgtattgg
      661 gctccgacgc ttgcccttgg tgggacctta tttgctctac cgaaagagtt gactttggat
      721 tttaagactt tatttgcagc tattcaaact ttgccattta agatttggac ttcgacacca
      781 tcatttgtag atatggcttt gctatctcgt gagtttgatg gggaacattt accagatttg
      841 acgcatttct attttgacgg agaggagtta actgtaaaaa cagctcagaa gctccgcgaa
      901 cgttttccaa aagctgtgat tgttaacgct tatggaccga ccgaagctac agtcgcctta
      961 tcagcagttg ccatcactga tgagatgctg gtgaattata aacgtttgcc gattggttat
     1021 accaaaccag actcgccaac ttttattttg gatgaggatg gtcaagtagt accgaatggt
     1081 caacagggag aaattgttgt atcaggtcct gctgtctcta aaggatattt gaataatcct
     1141 gagagaaccg aggcggcatt tttccagttt gacggcttac aggcctatca tactggggat
     1201 ttgggtgtaa tgacagatga aggtctgtta ctctatggtg ggcgtatgga tttccaaatt
     1261 aaatataatg gttatcgtat cgagttggaa gaagttgccc aacacctaaa taaatcaacc
     1321 tatattgagg ctgcggtggc tgttccacgt tataatgctg accataaagt gcaaaatctg
     1381 ttagcttatg ttgtccctaa agcagggatt atggagcagt tcgatcgaga gttggagtta
     1441 accaaggtca tcaagcagga gttaaaagaa cggatgatgg attatatgat gccttcgaag
     1501 tttatttatc gagacagtct tcctttgact gcaaatggga agattgatat taaggggttg
     1561 atcgctgagg tgaatgctca atgatggagt tttttaaaca gctacctcag ttagagcctt
     1621 atggaaatcc attgtacttt ttctatctag tcttggcgct agtaccaatt tttataggac
     1681 tcttttttaa aaaacgtttc cccttgtatg aaacaggtgt tagtttagcc ttcattgtct
     1741 tgatgtttac aggtacaaag acaatgcaat tgctgtcttt attggcctat attatttggc
     1801 agacaatgtt gatttttttc tacaagcatt atcgtcaacg ggcaaatcaa agctgggtct
     1861 tctatctaat cgtgtgttta gctgtttttc cgttgacttg ggtaaagttg gcaccgactt
     1921 ttagtcagca tggggcaatt ttcggttttt taggtatttc ttatttaacg tttcggtcgg
     1981 tcggaatggt gattgaaatg cgggacggtt tactaacgga atttagtttg ggaagttttt
     2041 tgcgattttt gctatttatg ccaactattt cgagtggccc tattgatcgt tatagacggt
     2101 ttacggaaga ttacaaacaa attcctgagc gaactgagtt attaaatatg ctcgatcaga
     2161 ctgtgcatta tattatgatg ggcttcttat ataagtttat tctagcctat tttatcgggc
     2221 atactttact ggagccttta aaggcagtag ccttggatca aggtggatgg tttaacttac
     2281 ctactattgg agttatgtat ctttatggtt ttgaattatt ctttgacttt gcaggctatt
     2341 ccatgtttgc cataggtatt tccaatctta tgggtattcg ctgcccaatt aactttgatc
     2401 aaccgttcaa atcgcgtgat cttaaggagt tttggaatcg atggcatatc agcttatctt
     2461 tttggttccg tgatttcgtc ttcatgcgtc tggttaagac tttgttgaaa cataaggttt
     2521 tcaaaaatcg aaatacggta tctaatgttg cctatctgct aaatatgctc ttgatgggct
     2581 tgtggcatgg tgtaacatgg tattatattg catatggtct ctttcatgct ttggggttga
     2641 tcatcaatga tgcttggatt cgtaagaaga aatcaattaa tttagctcga aaaaaagctg
     2701 ggcaagaacc attgccggat aatcgttgga cgagtttcgc tggtatgttt gtgaccttcc
     2761 atacggtcat gttctctttc ttgatatttt caggattttt agataaattg tggttcaagt
     2821 agacaatagg taacgattgt atgaatcgta gaaaggaata aaaaatggat gtaaaagaaa
     2881 caatattgaa tattatcgaa gaattattta tggaagatgt atctgaaatg atggatgaag
     2941 acctatttga tgcgggtgtt ttggatagta tgggaactgt agaacttatt gtggagttag
     3001 aaagtcgttt taatattaca gttccagttt cagagtttgg tcgtgaagat tggaatacag
     3061 ctaacaaaat catttctggt gttgtggagt tgatgcatgc gtaagcaatt gtggcagatt
     3121 tttggacctg tgatttgtgc aacgttttta gttattctca cgttgttgtt tttcccgagt
     3181 tcttctcggt caaatttcaa agaagaaaaa caagatgcag tagcattgtc ttatattagt
     3241 tttaagagca aatataagaa aaatagggct ctgggagata aaaatcatcg ttttgttcct
     3301 ttctttgggt caagtgaatg gtcacgtttt gatagtatgc atccgtcagt gttggcagat
     3361 gcctatcaga gagagtatac tccctatttg ttaggacaac gtggtgcagc ttcgctgaca
     3421 caatattttg gcatgcaaca gattggggca catttaaaaa atagtcaggc tatctatgtt
     3481 gtttctcctc aatggttttc acccaaaggg gcatcatcta aggcatttga acaatatttt
     3541 agtagtgatc aagccactca ttttctgcaa acaagtgcta aggatgacta tgatagatat
     3601 gcagcaaagc gtttcttaga gcttaaatca gactgtagtt tgacaggaat gtttcaaaaa
     3661 gtagctagtg gtaaggaatt gtcgaaatgg gatagagagc agttgaactg gcaagcctat
     3721 ttattagaaa agcaagatgc cttatttagt cgctttgctg attctggtag taatttagga
     3781 ttggtagaaa cggaagcgat gaaacttcca gagacttttt cctatgaccg cttgagtaaa
     3841 gtggctaatg aggacgggag tcgttctact caatcaaatg atttccaaat tgatgatagg
     3901 ttttatagaa atcgtcttca gtcaaaaatc aaaaaactca aacaatccca gacaaaaata
     3961 tcttatctgc aatcaccgga gtacaatgat tttcagctgg ttttgaatga atttgctaaa
     4021 catcagactg atgtgctatt tgtaattccc ccggttaatg aacgttggat gaactacact
     4081 ggtctcaata aagatatgta tcgggcaaca gtagcaaaga ttaagtacca gttgaccagt
     4141 caaggtttca atcatattgc tgatttgtcg gagcaggggg ggatagatta ttttatgcaa
     4201 gatacgatcc acatcggttg gcaaggctgg ctggagcttg ataagtatgt ccacccattc
     4261 ttgacagaac aacaagcacc tgttcattat cagttaaaaa aagaattttt aacttccgaa
     4321 tgggctgcat ataagggaga tttaaactta tttttgaaat agttagatac agttataata
     4381 aaaagcaaga aggctttgaa ttaggaagtc tccttgcttt tttgttcttt tactgtaata
     4441 aactgaatga ttacatatct tctcccaaca aactgtggtt ggctttgccg aagcgaataa
     4501 catgataggc aaccatagcg tgtgagagca ggtttaagat aacaaatagt aggatatttg
     4561 aatgaccatc cagcaataag gcaacatttc caattagtaa aatgagagca tagatgaaca
     4621 gccctctcct gccaacttga tggacttgtt gattatagaa ggaggtcgaa aggataccac
     4681 tatagaagat aaggtttcct acaatcagca tgggcaaggc caaatcatta tgatgactag
     4741 ggaaaagctc cattgccact gtgaagaaat tcaagccaag aacaattacc aagtgagcgt
     4801 atagcagaac agtcgcatct gccttgcggt gatggtcgat agtgagatag gtttgagaaa
     4861 tatagaagat gaacagagtc gccattgcga agaagaggag gattccttcc aaaggaagtt
     4921 ctagaagcgg gtaatttttg ataatagcta tgacgatctc accgaaagtg ataatggtaa
     4981 tcagctggca acgttccacc atgtgtggga agttgatggg gtcatgactc attctctttt
     5041 ggaagaagaa ggaaatcaag aagggaataa tatagacaat cagcatccgt tcatcattgg
     5101 tccatagctt gaagatgacc aacaaggttg caccggcaaa gacaacaata taagtcgcca
     5161 gcatctgcaa ggttgccttg atgtcctcat taaagccaat ccgtcttcct cttaagtaat
     5221 attgtaggag aataatgaca taagacaagg ttaaaaaggt gttaaatggc agagctgttt
     5281 gtgcaaaatc gttgctaaaa ttaagtgaca tatttccaat cagatacatg gatggaataa
     5341 tggtgaaaat atccagaaaa tcccgttcgc cgtatttatt gagatagatg gtttcattca
     5401 tccacaaggt cataataatc aagtttgtag tgataaatgt tgtaacttcc ttgaaaccga
     5461 tgtggttccc atgtagcaag ccagtcagtg aagaagtggc caggacaaag accagatcat
     5521 aaaataattc ataattggaa acacgttttg ctgtaatttc agacatgtca atctcctttt
     5581 aaaaaaatct gaaatattat accatacgaa aagggaggat gtcatctctc tttatatctt
     5641 atagtgtcca gtccaagtct ggtccgactg ggacaatacc tgttgggtta atggttttgt
     5701 ggctaccgta gtagtgctgt ttgatatggt caaagtcaac cgtctctgca atgccagcat
     5761 ggttgtaaat ccgtttggta taattccaca agtttggata atcaaccaag gccttgatat
     5821 tgcatttgaa atgcccaaag taaacaggat cgaatcgcac caaagtggta aagagtcgaa
     5881 tatcagcctc tgtcagctga ttgccaacca gatagttctt atcagccaaa tgctcttcta
     5941 gtttatccaa ggcagcaaaa aggttcttga cctctttttc atagactgcc tgactggttg
     6001 aaaaacctgc cttgtagact ccgttgttga tgtttggata gacaaaatca ttcatggcat
     6061 caatctcggc ttgcaggttt gctggataat agtcatcgta atttccagta atatcattaa
     6121 aagcagagtt aaacatgcgc atgatttcag ccgattcatt gctgacgatg gtattggttt
     6181 tcttatccca caagacaggc acggtcaccc gaccagaata gtgtggatca gccttcaaat
     6241 agacctgata gagataatcg ctgttaaaga gggtatcctt gataacacca ggaccatctt
     6301 caaatgtcca gccattttct aacatgaggg gatgaacgac agacaaggaa atatggtctt
     6361 ccaagccctt caattttcgc ataatcaagg tccgactagc ccaaggacaa gccagagaaa
     6421 tatagaggtg gtagcgacca gactcagcct taaaaccacc ctctcctgtt gggcctgcct
     6481 gtccgtctgg cgtaatccag ttgcgaaatt gtgtgacgct acgaataaac ttaccgcctg
     6541 ttgacttggt gtcataccat tgatccaccc atttgccatc ttgtaaaagt cccatgaatt
     6601 ttctccctta ctgaaaaaat gggaaatcca ataggatgat tggatttcct ttgtaaaaaa
     6661 atatatcact aactagtatt ataaactttt ttcaaaaaga gtcaagctat atgatttgtg
     6721 ataaaatagt cccatgagat tggacaaatg tttagagaaa gccaaagttg gctcgcgtaa
     6781 gcaagttaaa aaacttttca aagcccagca aatcaagatt aacggacaag cagctcaaag
     6841 cctaagccag attgttgacc cagagttaca aactatccaa gtttcgggta aaaaagttgc
     6901 cttagaaggt tccgcctatt atttgctcca taaaccagca ggagtcgttt ctgccgtgac
     6961 agaccaggag catcagacag tcatcgactt gatttctccg caagacagta gggaaggtct
     7021 ctacccagtc ggtcgcctgg acagggatac cgaaggccta gtcctcatta ccaacaatgg
     7081 tccgctcggt tatcgcatgc tccatcccag ccaccatgtg gacaaggtct actatgtgga
     7141 ggtaaatgga tgcttggctg aggatgcttc aaaatttttt gcctcaggtg ttactttctt
     7201 ggatggaaca cggtgtcagc ctgcagattt gactgtcttg gaggcttctt tggaccatag
     7261 cagagcgacc atcaagctgg cagaaggtaa gtttcatcag gtcaaaaaga tgtttttagc
     7321 ctatggtgtc aaggtcactt atctcaagcg gatttccttt ggtggttttg aactggggga
     7381 tttggaacga ggtacataca gacaactcac accaaatgaa atggaacatt tattcactta
     7441 ttttgactag aggaggatat ataatgagca tttatgattt tacagtacaa aaacaagatg
     7501 gcacagacca gtccatggct gagtatcaag gacaagtttt acttatagtc aatacagcgc
     7561 caggttgtgg attggcacca caatacaagg aactacagga gctatatgat agctataaag
     7621 acaacggttt tgtagtgttg gatttccctt gtaatcaatt tttaaatcag gctcccggtt
     7681 ctgcggaaga catcaatcag atctgtagct tgaattatgg tacaactttt cctcgttttg
     7741 ctaaaattgc tgtgaatgga tctgaagcta gtccgctata tcgctatctt aagaaagaaa
     7801 aatcaacctt attaggaggt cgaattgagt ggaattttac caagtttttg gtggatagac
     7861 aagggcgtgt ggtcaaacga tatttaccaa caactagtcc attaaaactt aaagaggata
     7921 ttgaacttta cctagaaaaa taacaagaag gctggagtca tctagccttc ttgttttagt
     7981 aaccacagaa aatagggagc gccgataagg gagacaatga tgccggttgg gatgcctgtt
     8041 ccgacaagta gtactcgtcc tatggtatcc gctagaagta gaatgaagct tcctattatc
     8101 atagtagctg gaatgatgac acgatggtca tttcctaacc atttgcgcac tatgtgacca
     8161 gcaatgagac caacgaaggt gatattacca actaaaatga cactaagaga ggctagggcg
     8221 gtagccaata tcaaggtaat ccgcctttca cgctgtaagt ggagccccaa agccatggct
     8281 gtctggtcgt ttagattcat aatattgagt gagcgacttc gactaaacgt tgccatccac
     8341 accaatagaa gaagaggagt atagattgcg atagttttcc aatctccacc atttactcga
     8401 ccgctgagcc actggacaat gtattccatc ttaaagtcat ctaattggct gatgatgctg
     8461 accatgatac cagacagcat gctcgaaatt ccaactcccg tgataattaa gcgtgcagga
     8521 tggataccgt acagcttctt acgtgcaatc cagtaaacta gaaaaatggt taatccaccg
     8581 cccatcatag caaatagagg catgagactc aataagacag ggctgatttt tgaagagaga
     8641 ccgacaaata gtgccacaac aagaccagct cctgcgttga ttcctaatat tccagaatct
     8701 gccagaggat tgcgggtcaa agtttgtagc aacaaacccg ccaaagccag agaagcccct
     8761 ccgataatgg aggcgattat tctaggtaag cgaattcgtc caatgataag tagagtcgat
     8821 gaactggcac gcccactcat gatgtcgatg aaatccgaaa aggatgaatt gctatagccg
     8881 atagataagg aaagtaggaa aatgataaat aaaaggatta gcaggaatgt gaatattttt
     8941 agatagttgt gatgtcttat caaaagtgtt tctcctttct aatcaaccat aggaagcaag
     9001 ggagaccaat gatactgatg actgcgctaa tgggagtttc aacaggtgga ttgatagaac
     9061 ggcaaaccaa atctacccag atgaggaaac tagcccctgc aaaggcagtt aatggcaaga
     9121 gtagcttgta atttttccct gtaaacatgc ggataaagtg aggaataatg agcccgataa
     9181 aagaaagtga gcccacaaga gctaccgcag aagctgagag tagaaggaca attcccaaca
     9241 aaaccaaggt catcagtaag gttcgttgac ctagattttt agcaacggtt tcgttgagac
     9301 tcaggatggt cagctgatgc gaaaagagtt gagcaaggat gaggccgatg atgataagag
     9361 gagcaatgat ggccagcatc ttccagttta cctgtaccaa tcccccagct tgccagccaa
     9421 tcactgcagt tgataaatca aagtagatag taatagcttg accaatagca gaaaatagac
     9481 ttgctaccat ggcgccagat aaaattaaac gtagttgatt gtagcctttt tgcacctgat
     9541 aagctagacc aaagactagg ccggctgcca gacaggagcc cagtaaacat attaataaaa
     9601 tttgactgta gtgcaaacca ccgaacagag cataggcaac aatgagagca agtcctgctc
     9661 cagcattgat tcctaataag ccaggatcgg caatcggatt gcgtgtaatt ccttgcatca
     9721 ttgccccagc ttgggccatg gcagctccta ccaagatggc ggctaccatt ctaggcaagc
     9781 gtaaatcgat gataacatcc tgagcggaag aatttgtcag tggatgcttt agggtttcta
     9841 tcagttgttg gtggctataa gaaatagctc caaagcgtaa actaagatag actccagcta
     9901 tgaaagaaag acagatgatg aaaaaaacaa gccaaatatt ctttggcttt gttttgggat
     9961 aacgagaact tactagtttg ttcatatatc cagtcctttt actgaagttt ttgaatttct
    10021 gagacaaagg cgtcaatttg taaatctaaa gacatcggat cggagaagta gaagaggtta
    10081 tagtctatct cgatgacgtg accattcttt acagcaggta gatttttcca aatatcactt
    10141 tctttgagag aggcagctgc ttcttttgtt ttatcattga cgttaacaag gacatagtcg
    10201 ccaacatatt ctggaagagc ttcttgagat accccaaacc agccttcttt attgatgatg
    10261 tcttcttgaa cctttgcagg agcggcaaaa ccgagagcgc gatagattaa ttctccacca
    10321 cgaccaaagt tattaccata aaggaagatg ttcttatcga agaagtccat gacagtaaat
    10381 gttgaacttg tatcaatcaa tcctttcaat tcctcttttg cagtagtaac tttcttatcc
    10441 cattgatcta accaggcttg tgcttccttt tcttttccaa aaatctgacc aatatctgtc
    10501 agcattttca agtagtcact ctttccataa gtgatttcaa taactggagc aatttctttc
    10561 agtgtttcta agttatcatc accagaaaat actagaatga catctggctt ttgtgcggca
    10621 atcgcttctg tatctgctgt tgttaattgt ggaacatcct ttagtttgtc tccaaagacg
    10681 ggactatttt tctctaaatc tagactatag ctagatacat caattcctaa ttgtaagaga
    10741 tagcctgtgt aagagtaagc aaagttgaca acttttttag gattttttgg aatgtcaccg
    10801 tggtaagtaa tgccttcaat ctttggcatg ctagacagtt ctacgtctgt tgaagaggaa
    10861 gaggtagagc aagctgttaa gaaaaccagc ccaacaaata aactaaaaat agctaaaaac
    10921 tttttcattt tttctcctta ttattttttt aaatcgtaag ttagcaaaat agggtaatct
    10981 tgaccagcaa cctgcatcaa ttgaggttca atgctaaaaa tgtccctaag tatctcagtt
    11041 gtcatcatat tggacacaga tccatggtag cgaatatctc cctctttcat ggcaatcata
    11101 taatctgaaa atcgagctga taaattgaca tcgtgaagga ccatcacaat ggttttctgt
    11161 cggctttgat tcagttcttt taaaagttct aagacttcca attgatgatt gagatcaaga
    11221 taggttgttg gttcatctaa gaaaatggta tccgtatctt gggcaagtgc catcgcaatc
    11281 caaacccgtt gcctctgacc acctgataaa tcatcaactg gtaacctagc gtagggagcg
    11341 gtccgggtga cttccaaggc ccagttgatt ttctctctat cctgatcagt gagatgtccc
    11401 agcccatttt gatgcggaaa gcgaccatag gagaccagtt cataaacgga aatcccttct
    11461 gtcgcttcta agacctgagg gagcagggcc aatttttttg caacttcttt ggttggcagt
    11521 tgtgaaatgg cttgtccatc tagatagata gccccttctt gaacaggaat aatccttgtc
    11581 aacgccttaa gcaaggtcga ttttccacag ccattggcac caatgattgt cgttatactt
    11641 ccttttggaa tacttgttga taattcatca ataataatcc gattgtcgta agagacttgg
    11701 atattttccg cttgaatcga agacacaaac tcctcctttc cagaatatga ttcattaata
    11761 atttgttccg accagtatta tatcaaaaaa atttaccaaa acaaagagtt ttctgaaaat
    11821 tttaatttaa tgagaatcgt tataaaaaag tccttgtttg tgaacaagga ccgtatgtta
    11881 caaatattgt tttgctactt ccaaatcacc gtcataggcg gccttttcgc cgtttacaat
    11941 ctggtaggca tcggctccct tgcctgcgat gatgacggca tctttcggag agcttgtctg
    12001 actcatggct gtgcgaatgg cttcttcccg gtccaagata aagttgcttg tacgggtcat
    12061 gtgggcacgg atttgttcag caatagcagc tgggtcttca cggttggggt catcagctgt
    12121 cagaatcacc tcaatctgcg gatagtcatt gagcagaaga ccgaagtctt tgcggcgact
    12181 ttctcccttg ttaccaggag ctcccaaaat caaaaatacc ttgcctgtct ggtgctctaa
    12241 gacgacatca atcagcttct tgacactatc gccgttatgg gcgtagtcca cgaatacctt
    12301 ggcaccgttt tgctgggtca ggacttccat acgaccaggg acattggttt gggcaatccc
    12361 tgtgtgaata tcttccagac ttgctcccaa acggagacag gccagtcctg ctgcgatggc
    12421 attttcttgg ttgaagccac cgatcagctg gatgtcgtaa tggccagcca atttgccagt
    12481 cgctgtaaaa tcaaaacctg ctgactgact aatctggttt tcagaagtcg gcccataaaa
    12541 gtcatggtct ttggagctga cttgctcttt cacgacttca aaatggtcca ttccagcatt
    12601 gacaatgacc gctctgctat tgtccatcaa aaggcgcttg tgatagaaat agtcctcaaa
    12661 ggtcggatgc tcaatggggc cgatgtggtc ggggctgatg ttgaggaaga caccgacgtc
    12721 aaaggtcagc ccgtaaaccc gcttgaccaa gtaagcctgg ctggacacct ccataatcaa
    12781 gtgggtcatg ccgttcttga ccgcctccgc catcatggca aagaggtcca gactttcggg
    12841 cgtggtcagg gtggacttga agaaggtctt accgtccagc gtggtgttca tggtcgaaag
    12901 catggcaggt ttcttgctct gtttaaggat gttaaaagca aaataagccg ccgttgtttt
    12961 acccttggtg ccggtgaagg ccaggagttt gagctgtttc tggggatgac catgaaattc
    13021 catggcaatc aggctcatag cctgcttgat gtcgtggacg atgatagcag gaatgccgac
    13081 ctcatagttg atttcagaca cgtaaaaagc cagtccggcc tcgatagctt tttccagaaa
    13141 ttccttttta aaggccaaac ccttggcaaa aaagagggtc attgggctaa cagtccggct
    13201 gtcatagctg agagcgtcaa actggacatc cgtccatgtg ctgtcagttt ggtcattctg
    13261 tttgatatgg cggaagttag catccgcctt caatatttct aatacacgtt caattgtaat
    13321 catgcttcta ttgtaaacct atcgaggata tttgacaagt ctgggatatg taaatagtgg
    13381 gtattttgac cgtaaatttg aatgaatatg acatttcgcc ctgaagcaaa tttgtgctat
    13441 aataggaggg attgaaaaag gagaagttta tgttttattc ttatctacga acactattaa
    13501 cattcttgtt gtgggccatc aatggcaata ttcactatca tgatagagag aagattttgc
    13561 cacaggagga aaattatatt cttatcgctc cgcacaaaac cttctgggat ccagtctttc
    13621 ttgggtacgc agctgctccc aagcagttca tctttatggc taaaaaagaa ctcttcaaag
    13681 accgcggttt tggttggtgg atttcaaaat gtggagcctt tccaattgac cgtgacaatc
    13741 ctggcatggc agccatcaag tatccagtta acatgttgaa aaaaagtgac cgttcgctgg
    13801 tcatgtttcc ttctggaagt cgtcattcat ctgagctaaa aggtggtgtg gcggtcatcg
    13861 ccaagtcagc caaggtcaaa ctcatgccag ctacctatgt gggtcccatg actatcaagg
    13921 ggctcttagc tggtgagcga atcgatgtgg cttttggaaa tcctatcgat gtttcggaca
    13981 tcaagcgtat ggatgatgca ggtacagcag aagtaactag ccgaattgag gctgagttca
    14041 aacgcttaga tgaccatgcg gcatctttcc aaaccaagaa aaaaccaaat atcttgactt
    14101 atatctatcg tatcccagtt cttttgctgg ttgctcttat cttgggattg acctatgcct
    14161 ttagctatat tgccagcttc ttctggcaac caagtactca gttggataaa aataagtaaa
    14221 tgaaactcgc agcttgcggg ttttttgtat tttttacgaa ctatataggt ggaggtaatt
    14281 tatggatacg attaaaactt atatagaaat gcttaaagaa tacaagtggc aaattgctct
    14341 gccagcagtg gctggcttgc taatggcgac gtttttaata ttcagccaac ctgctaagtc
    14401 tgatcaaaca ggactgacag attttcctca aaccgagcaa gcttctagca gtcaggagca
    14461 gacggaggaa accagtacag aagagagtga ggagctcagc cagctaaccg ttgatgtcaa
    14521 aggagcggtg gaaaagcctg ggttatatac tttagaagct ggtgcgcgtg tcaatgacgc
    14581 agttgaagca gctggcggct tgaccagtca ggcagacccc aagtctatca atctggctca
    14641 gaagctcagc gatgaggcgg tggtctatgt agccagcaag gacgaaaaca tctcggtggt
    14701 ggccagcacg actgccagct ctgctatgtc tccagaagaa aaaagcacca gtctggtcaa
    14761 tctgaatacg gcgactgagg cggacttgca gaccatttcg ggtatcggtg ccaagcgggc
    14821 ggcggacatt atcgcctatc gtgaggcaaa cggtggcttc aagtcggtga acgacctcaa
    14881 caatgtttcg ggcattggcg acaagaccat ggaaagcatt cggccttatg tcacggttga
    14941 gtaagctccc ctgccagccc gttcacctgg cagtcttggc ggtggcagct tactttgcag
    15001 tccactcttt ttccctcttg acaatgtgcc tgctcagctt ggtgttagct gtctttggtg
    15061 ttcggcaagg taagacagtt ttcctcaaaa cgctgccgct tctaaccctg tgtggtctct
    15121 ttttcggatg ccaaaaggtc caatgggagc gggcagctca gtcagcccca gagcaagtga
    15181 cgactgtgca ggtcattcca gataccatag acatcaatgg cgacagcttg tcttttcgtg
    15241 gtcgggctga tggacaaaac tatcaggttt tctacaaatt aaccagtcag gaggagcaga
    15301 cctattttca aaatctggca gacctggtcc agctagaagt ggaagcagag gtcagcctgc
    15361 cagctggtca gcggaatttc aagggctttg actatcaggc ctatctgaaa acacagggca
    15421 tctatcggac tgtcaagcta acggcgatta agaagattgt ccctgtccag tcttggaatg
    15481 tttttgactg gctgtcaact tggcggaggc aggccttggt ctatgtcaaa accaattttc
    15541 cggctcccat gagtcactac atgacagggc ttttgtttgg ggagctggat agtgattttg
    15601 accagatgag tgacctctat tccagtctag gaatcattca tctctttgcc ctgtctggta
    15661 tgcaggtggg ctttttcatc gacaagtttc gttggatttt gttgcgtctg ggcttgacca
    15721 aggaaacagt ggataagtta caaattccct tttcccttgt ctatgctggt ttgacaggct
    15781 tttctgtgtc ggtggtgcgg tccttggtcc aaaaaattct gggtaatcta ggcttgcgga
    15841 agttggataa ttttgcggtg acggtcttta tctgtctgtt gatactgccc cgatttttac
    15901 tgacagcagg aggagtgctg acttttactt atgctttctt gctgacggtc tttgattttg
    15961 aggatttggg gcaggtcaaa aaggctgcgg tggagagcct cagtatttca ctgggaattt
    16021 tgccggtgct tatgacctat ttctatgcct ttcagcctct atctatcctc ttgacctttg
    16081 tcttttcctt tgtctttgat gtcctactct tgccaggttt gtcagtcatt tttctcctgt
    16141 cgccagttgt caagatgact tgggtcaacg gatttttcgt ctttatggag aaaatcattg
    16201 tctgggtggc tgatttggga ttgcggcctt ggatactggg caagccgtct gagctggtcc
    16261 tcttgctttt gctggtcagc ctcttcttgc tctacgattt ccacaggaga aagaaatggc
    16321 ttctaggact gagcttggtc ctcgctctgc tatttttcat caccaaacac ccgctggaaa
    16381 atgaggtgac ggtgctggac atcgggcagg gggacagcat ctttttgcgg gatatgcggg
    16441 ggcggacggt tctgattgat gtaggcggac gagtcgattt cgcggccaag gaagcgtggc
    16501 aggagcggtc tcggcaggca aatgcagagc ggactttgat tccctatctg catagtcggg
    16561 gtgtggatag gattgatagt ttagtcctga cccacaccga cacagaccat gtgggggatg
    16621 tgctggaagt ggcgaagcag gtccagattg gtcggattgt cgtgtcgccg ggaagtctga
    16681 cggtgcctga ctttgtagcg accttgaaaa aaatcaatgt tcctgtccat gtggtaaaag
    16741 tgggcgaccg cctaccaatt tttgactcct atctggaggt cctctatcca gacgggacag
    16801 gcgacggtgg caataatgac tccattgtcc tttacggtcg cttgttggaa actaactttc
    16861 tctttacagg agacctggag caaggggagt tggacttgat agaaacctat cccaacctgc
    16921 ctgtcgatgt cctcaaagcc ggtcaccatg gctccaaagg ctcttcttat cctgaatttt
    16981 tagaccatat cggtgccaag attgccttgg tatctgctgg agaaaacaac cgctacaagc
    17041 atcctcacca agaaaccttg gaacgatttg acagccggaa tatccaagtc tatcgcacag
    17101 accagcaagg agccattcgc ttccgaggtt ggaaggagtg gaagattgag acggtgagga
    17161 ggtaaaatga taaatcctat gcacttatgg ttctatagtt tcaaagttta tgtgctcgct
    17221 gccttatctg gctatattct gcactatgtt actacgccag cttctgttaa cgacttcaaa
    17281 gaggttgctg ccttattgga aagatgctaa caatctgtga ttgtagcaga tttgggctat
    17341 ctcagtcagc agtcttgctc attccattgt atttcaaata gtctatattt tgaatttaag
    17401 aattgatttt catttctttt cattaaatat tgaaatcgtt ttcgaatttg tggtataata
    17461 aagtatgatt taccaaagga ggaagtccta tgtctgttaa agattttatg acccgtaaag
    17521 ttgtttatat ttcaccagat acaaccgttg cacacgctgc agatattatg cgtgaacagg
    17581 atttgcaccg tctacctgtt attgaaaatg ataagttggt tggtttggtc actgagggaa
    17641 cgatcgcaga agctagtcca tcgaaggcaa ctagcctatc tatttacgag atgaactacc
    17701 ttttgaataa gactaaggtc aaggatgtga tgattaagaa tgttatcaca gtctcaggtt
    17761 atgctagtct tgaagatgct gcttatctca tgtataaaaa caaagtgggg attcttccag
    17821 ttgttgacaa cggtcaacta tatggggtca ttactgatcg tgatattttt gcagcttttc
    17881 ttcatgtctc aggttatgga gaagaaggag tacgcgctcg gttccttgtt gaaaataagg
    17941 caggggagtt agaaaaaatt attcgattgg tggcagataa ggattataat attgtatcta
    18001 ctgtgcaact tgctaccaag tccggaaaag ttgtcatcga agtgcagatt gacggaaaag
    18061 ttgatgttga agaagttcga actcttttcg aaggtgcagg tatccaagtg gatagcttgg
    18121 ttccgactgt tgctaaaaat atttaaaaac cgagatttct cggttttttt ctttagctat
    18181 cattctagta ttttagggga atgtgatata ataaagtata tcatgtgtga ggaggctcta
    18241 tggggcacaa atataaaaag tataagaaat atggtaaaaa taattcagga cttgtggccc
    18301 tccttgtttt attggcaatc ggttttgtca tgagtttctg gtatatctta ttgcccactg
    18361 ggctcttaat tggctatttt tacaagagag aggctatcca gcgttttttg aatagagatt
    18421 ctataaaacg cttgcaagta ttagcgctat ctattcgttc gggctatgaa aagcatcaag
    18481 agctcttggt ggaaaatggt gaggataagg ttgtattaca gttgagaaac agactgttag
    18541 gacaattatt tgaattagat cagctctatc aaaaatttgg aaaatatctg aatggatatg
    18601 aaagtaaaga agttgtagat accttgcaac taaaatatca tttaaaactg ccagaagcta
    18661 aaacagaaaa aaatactgac acacaggttt ctactggtgg taatgctgtg atggatgaac
    18721 aaggcatgat agcggattta gctcctgaaa ttctagaaac ctattgtaat attcaaagag
    18781 acaatcttgt tattcttgaa aagctggaaa aggcgacaga taagcgggaa gaattgacag
    18841 ctatccatga ggccaatatg aatcggttta acgatatttt acagggttat ttgaaaatca
    18901 aaaaatcacc gaaagattat tttaacgcag aagaacgatt ggtgcaggca aaatcagcca
    18961 tggaaatgtt tgataaggat ttggatgata ccatcaaaca atttaacgaa gcggatatga
    19021 aagattttga agtcagtctg cgtatgatga aaagagaaga ggaatagaga agatgtcagg
    19081 atttaatttc gatattgatc agattgctaa taatagttta tcaaccaaag ataagacaac
    19141 agagcttatt caagcgactc cgactggaga taattcaaaa gtgtctttct tggcacagtt
    19201 gacaccggaa caacaaacag gtattcgtgc caaggctccc caattggtgg ataatttctt
    19261 agctaatcaa aatgtcctct tggattttgg taaggaagcg gtagaagaag tcaatgccac
    19321 ggtcaatcac attttgtcag aacagaagaa gattgaaatt ccacaggtcg atgagctttt
    19381 ggccaatacc aaccgtgaat tgaatggttt tgtggctaag tataaggaca tttccacaac
    19441 tgctgaatta gaaaagaaac ctggtttctt ccaacacctg ttcaagcaga ccaaaaatga
    19501 tttgcaggaa ttttatttcg attcgcagac cattgaaaag aaaatggata gcatggcagc
    19561 cagtgtggtc aagcaggaag aggtgttggc gcgcaatatt gtttctgcag agcttttgat
    19621 tgaaaataat accaagtcta tcgagaattt ggtgggggtc atcgccttta ttgaagcgac
    19681 tggtcaagag gctgcccagc gagcaaaagc agagcaagaa cgcttggcag gtctgcaacc
    19741 aactacggta gattatcaag tagcttctga gaagttggca cgtgtaacag aggttgctaa
    19801 tatcctcgaa caacaacatt ctgagtatat gagtcgcttg tatgtcgcgt ggacaacgac
    19861 accgcagatg cgtaatctgg tcaaggtttc ttcggatatg aaacagcgcc ttgggatgtt
    19921 acgtcgcaat actattccaa caatgaaatt gtccattgca cagttgggca tcttacaaca
    19981 atctatcaag tctagccaga cggcagatgc gattgtcaat gctaataatg ctgcccttca
    20041 aatgcttgcg gatacatcaa aagaagccat cccgatgatg gaacgcacag ctcagaatcc
    20101 gaccctttca gtagcatctg ttaccaaatt ggcagaaagt ttggttgccc aaaataatgg
    20161 cattattgca gctatagatg aagggcgtca gaaacgtcgt gagttggagg cagctattgt
    20221 acggtcagca gagactatca acgattctgt taagctacgc gatcaaaaaa tcattgctgc
    20281 tttgctggag caaggcaaag aagcacagtc tgaggcagag caaccacttg aaaccaatca
    20341 ataatataaa acctgcagtt cagcgaaact gcaggtttgt ttaatggcgt tctattggtt
    20401 ttttatctcg atctaaagta aatccctcac cgagtacttc atgtgcgtct gtaatggtga
    20461 taaatgcgtg ggggtcaata cgatgaataa gctccttcat ctcttggagt tggctcttat
    20521 atatgaccga gtaaatcatg ttgacatcgg ctttgctgta gaaaccttgt gctttgataa
    20581 aggtgacacc acgctcgatt tcacaatcaa tagcctgagc caattggtca gatttttgag
    20641 aaacaatcat aacacccttg ctaccatatc cgccctccgt gacaaaatca ataaccttgg
    20701 aagcaatagc aaccatcatt aaagtataga gaactgttcg gaggtcttta aaaacaataa
    20761 caatcagagt taggatgagg atgtcaactg caagaatgat ttgtccaatg gagtaaggta
    20821 ggaatttatg acctatacga gcaataatat cgcttccccc agtcgttccg ccagcacgga
    20881 agatggttcc taatcctagt cccataagaa tcccacccag gatactaacg agtatcaaat
    20941 cttgttgtag attgattgaa aaatggattt tttcaaaaat ggccaaccaa aaggtaacgc
    21001 taaaggttcc taagatactg agatagagag tcttctttcc gagtattttc cacccgagga
    21061 tgaacaatgg aatattaatc acaatgttca ttatccaagg ttgaatatga aaaaggtagt
    21121 aaataatcag cgtcaatcca gttgcgccac cctcgaatag acgattgggc atgattaaat
    21181 aatttagccc aaaggcaaat agccctgcac caagcaaaat aagtaaaata tttttcaaac
    21241 gaaccataag catctccgaa tatgtgtata tatcaatttt aaaagatttt ctccttaaaa
    21301 acaagtcaat tttcaagtga ttttttgact aatttttggt aaaatggaat gaatatctaa
    21361 gaaccagtct tcacaagtga agtgctgaac tgtgcatact ggtactaaga gcgagggaaa
    21421 gatgagaaac ggttttttta ttacatttga aggtccggat ggggcaggga aaacaacggt
    21481 attgcaacag ttattgccag ccttgcagga attggggcca gaagtagtca caactagaga
    21541 gccaggtggg gtggctattg cagaagaaat tcgtaatatt attctgaatc ctgcaaatac
    21601 agcgatggat gataagacgg agctgttatt gtttattgca gcacgacgtc agcatttgaa
    21661 agagaagatt cttccaccct tggcacaggg gaagttgctc ttgattgatc gttttatcga
    21721 ttcctctatt gcctatcaag gatttggacg ggggttggat ataggtgaca ttcattggct
    21781 caaccagttt gcaacagatg gtttaaagcc agatttaact ctctattttg atattgatac
    21841 agaagagggg ctggctcgaa ttgcccgcaa tgctgaccgt gatgtggatc gcttggatat
    21901 ggaaaaagca gatatgcata gacgggtgcg tcaaggctat cttagtattt tagagcagga
    21961 accagagcgg tttgtaaaga ttgatgccag ccagccacta gaggctgttg ttgcaaatgc
    22021 tctggcggtc attaagaaac ggtttgcaga aagaacatga agattgaaga acttagaaac
    22081 ttacaaccct atgtatttca acgttttgtg acaattttag agcagagacg tttggcacat
    22141 gcctatttat tttcaggtga ctttgctagt tttgatatag caatttttct cagtcaatcg
    22201 ttattttgtc aggagaaaga aggcatcctt ccttgtcaga agtgtcggtc ttgtcgtcta
    22261 attgaagcaa atgagttttc agatcttact gttattgcac cgcagggaaa tgttatcaaa
    22321 acagatacga tacgtgaatt agttaagaat ttttcacaat ctggatttga atccaataag
    22381 caggttttca ttattcgtga tgctgagaaa atgcatgcta acgcagccaa ctctttgcta
    22441 aaagtgattg aagaacctca gtcagccatt cacatcttct tattgaccaa tcaggaagaa
    22501 gctgtccttc ctactatcaa gagtcggaca cagattatag gttttcctaa aaatattcca
    22561 gctatggagc gaatgttaga agaggagggc ttgttaaaaa cacaagcgaa tctcttagct
    22621 caactggttt caagtcaaga agaagcgaaa aagttagcag aaaataaaaa ctttttagat
    22681 ttgatgggac aggcaaagaa atttacagac ttgctcttgg tggagcctac acgtgcttat
    22741 ttgcaagttg gaatcctagt gtctttagct gtggaaaaag ctgagcaggg acgtttgttt
    22801 gaccttctga gtctgatatt atctgagaaa atgatggaat cggcaaacgt acgtgaaaaa
    22861 atggatgctg ttcttaaggc taagaagatg tggcaggcaa acgtaagtct acaaaatagt
    22921 ctagagtata taactctgta aaataaaaga aagggagata atggataaaa aagaaatttt
    22981 tgatgcccta gatgattttt cgcagaatct tctaaccacg ttggcagaag tggatgctat
    23041 caaaaagcat ttacagggag ttattgatga aaatacgacc ctccgcttgg aaaattccaa
    23101 gttgcgcgaa cgtttggaaa aagaagataa gacaggccat aaatcttcca actttggtaa
    23161 ggagaatttg gagaatattt acgaagatgg tttccatatt tgtaccttct cttatggtca
    23221 acgtagggat aatgatgaac cttgtatgtt ctgtgtagaa ttattgaatc gagattagga
    23281 tgaaagtaca aaaatcattt aagggacaga cgagttttgg gactttatat ctggtgccaa
    23341 cgcctattgg aaacttacag gatatgacat ttcgagctgt tcagattttg aaagaagtgg
    23401 atataattgc agcggaagat acgagaaata caggcctcct actcaaacat tttgaagtag
    23461 aaaccaagca aatatccttt catgaacaca atgcccatga aaaaattcca gttttgattg
    23521 attggctcaa atctggtcag tccattgcgc aagtttctga tgcaggcctg ccttccattt
    23581 cggatcctgg tcatgacctg gtaggggcag cgattgcgga ggatattcct gttgtagcac
    23641 ttccaggagc atcggcggga attacagcct tgattgcatc tggtttggca ccccagccac
    23701 atatttttta tggattttta ccgagaaaat cggggcagca aaaagaattt tttcaagaaa
    23761 aaaagaccta tcctgagacg caaattttct atgaatctcc atatcgtgtg gcagatacct
    23821 tagaaaatat gttgacagtt tatggtgatc gtcaggtaac ggttgttcga gaattgacca
    23881 agttatacga agaataccag agagggagca tttccgaatt actagagtat ttggaagaaa
    23941 atccacttaa gggagaatgc ttgattattg tcgagggtgc tggtgaagaa gtctatcctt
    24001 ctgctgatga agtggacttg aaggctgagg tagaaaggga aattgcaagt ggcataaaac
    24061 ctaatcaggc tatcaaagaa gtggccaaac gctaccaact caaaaaacaa gaagtatacg
    24121 atatatatca tggactaggt taagacctag tttttttgta tgtttatgca agcttatact
    24181 cttcggaaat caaaattatc cgttgtcaag agccttgatg agtgaaaagc tccagtggat
    24241 cttttcagcc tgttaccttg aaatgagaaa gtaacagagt tctatcttcg gcttaatgac
    24301 gcgaacacag ttctctatac ttccagcctt caactatatc ccagctaagc tacttttgat
    24361 tttcattgag tattaatatg cagatagcaa ttggtgacac tccccaaaca gtcaaatctg
    24421 gtcataaaat tttcattgaa acctgataaa tccctgtgag gcaagcgcct cgtttatggc
    24481 taaaagtaag aggtgcttta gaaagtagaa taaatataag aaatccgaat atttttacaa
    24541 aataatcata tttcatggaa ttgtctgaat atttgtgata taatgaacag actaacttaa
    24601 tggagggatt ttatgacaat ctacaacttc tctgcaggtc ctgcagtatt gccaaaacca
    24661 gtacttgaac gcgcccaagc tgaattcttg gactacaatg gttcgggaat gagcgttttg
    24721 gagatgtcgc atcgctccaa ggacttcgat gatattatta aaggtgctga ggcaacgctt
    24781 cgtgaattga tggccattcc agataattac aaggttattt tcttgcaagg tggagcttct
    24841 ttggaattca caatgattcc ccttaacttc gctcaaggca agaaagccta ctatcttgta
    24901 ggtggctcat ggggtaaaaa agcctatact gaggctgtga agctgtctaa aacaattgcc
    24961 tttgaaccaa ttttgcttgg atcaacggaa gatattacct atgcagaatt gccaaccttc
    25021 gataaaaatg atattgatcc aactgctgct tacgtgcatt tgacaacaaa taacactatt
    25081 gaaggaacag cagtttatga tattcctgac accaatggtg tgccagtcat tgctgatatg
    25141 tcttcaaaca tcttagcggc tcgctataat gttgaagatt ttgccatgat ttatgctggt
    25201 gctcagaaaa atattggtcc tgctggtgtg acggtggtga ttgttcgtga ggacttcctc
    25261 aatgatcaac caatgctttc aagcatgttg gactaccgta ttcaagcaga aaatgactct
    25321 ctttataata cgccaccagc ttattctatc tatatttcta aactcgtctt tgaatgggta
    25381 aaagaaattg gtggtgttga tgagatggag aaaatcaacc gtgaaaaatc tgggttgctt
    25441 tatgactata tcgaccagtc taatttctat acaaaccctg ttcgtaaaaa agaagagcgt
    25501 tcagtagcga acattccttt tgtctcacca agtgaagaat tagatgctaa atttgtcaaa
    25561 gaagcaacag cagctggttt taaaaacatc aaaggtcacc gttcagtagg tgggatgcgg
    25621 gcgtcgctct ataatgcttt cccacgtcaa ggtgtggttg aattgattga attcatgaaa
    25681 aaatttgcag tggagaatgc ttaatggaaa ttagattagc acatccaaat gagatggagg
    25741 ctattgtcaa tattcttgaa gatgctcgtg catttctagc aacctccggt atcaatcaat
    25801 ggcagggaga gtatcctaat aaagagaata tttttgatga cattctttct ggcagaggtt
    25861 acgttggtct agttgacggt aaggtggcag cttatgccgc cgttcatcgt ggtcaagaag
    25921 cagaatatga ggccatatat gaaggaaaat ggcagcacaa tcatcctgta tatacaacat
    25981 ttcatagaat tgcagcagct gacgaatttc gtggtcaagg aatcatacaa acttttttgc
    26041 aaggtttaat tgaaggtcaa aaaggaccgg atttccgctg tgatacccac gagcagaata
    26101 agacaataca gcacattcta aaaaaattgg gctatgtcta ttgtggtaaa gtcccaatag
    26161 atggagaacg tctagcctat caaaaaatca agcaaaagag cgagcggagt ctttaccagg
    26221 aaatcagtga agacgaccgt tggttactcg gaaataatta atgaaataaa gaaagaaaag
    26281 gcggcagcta ttccgccttt ttaggtaaaa ataaatggta tttagcgtac gtacttttaa
    26341 caatatcaat caaattggtc ttaaggaatt aggcaataaa ttccagattg acggagatca
    26401 tgcagcaaat ccagatgcct ttattattcg atctgaaaat ttgcatggat ttgattttcc
    26461 tgaaaatcta aaagctattg cccgtgctgg tgcagggact aacaatattc caatcgaaga
    26521 ggcaacagag aaaggtattg ttgtctttaa cactccaggt gccaatgcca atgccgtaaa
    26581 agaggcagtc attgcttcca tcctcttgtc agcacgtgac tatatcggag cgactgcatg
    26641 ggcaaataca ttgtcaggag atgatgtacc taagcaggtt gaagcaggta aaaagcagtt
    26701 tgctgggaca gaaatttcag gtaagactct tggtgttatc ggacttggtg ctatcggtgc
    26761 gcgtattgcc aacgatgccc gccgtttggg aatgaatgtt ctcggttacg acccttatgt
    26821 atcagtagaa actgcatgga ccatttcaag ccatgtcaaa cgcgtggatg acttgaaaga
    26881 aatctttacc aatgcggact acattactgt tcatgttcct ttgacagata agacacgagg
    26941 acttttcaat gcggatagct tttgtcaaat gagaaaagga acaaccttga taaactttgc
    27001 acgcggtgaa ttggttaata atgctgattt gtttgaagca attgaggcag gagttatcaa
    27061 gaactatatc acggattttg ggacagaaga agtgctcaac aaggacaaca ttatcgtctt
    27121 cccgcacgtt ggtgggtcaa cagaggaagc agagctcaac tgtgccattg ctgcaggtca
    27181 gaccattcgt cgttttatgg agacgggtga aattatcaac tcagttaact tcccaaatgt
    27241 gaaacaattc ctagatgcac cgtatcgtat taccttgatt aataagaata ttcctaatat
    27301 ggtggcaacg atcacaacgg ctgtatctga gttgggaatc aatatcgata atatcatcaa
    27361 taagtctaaa ggtgactatg cctatacttt acttgatttg gatgaagcgg ataaagtgaa
    27421 aattgagcag ttggttgcta aatttgaagc aacggatgca attgtcaaag tgcgtgtcat
    27481 tcagaataaa aactaatcat gctgtataaa caagtctatc acagtccact tggccccatg
    27541 tcattaattg ctagtgataa gggattaaga ggggcctggt ttgagggaca aaaatatttt
    27601 gaacgaggct tgaatgagaa agctatttta gcctcccatc ccattttaga ccagacagct
    27661 cgattgcttg accagtattt ttcaggggat caggtggact tttcaaacct tccattagaa
    27721 gctgtgggaa caaatttcca agagcatgtc tggcaattat tgaagattat tccgatgggt
    27781 cagacgacta cctatggaca actggctcag caactgggcc ttcgttctgg tcaggcagta
    27841 ggaggagcag ttgggcgtaa tccttattct attattgttc cttgtcaccg agttctcaat
    27901 cagaaaggac agttgacagg ttatgcaggt ggactggata agaaaatctg gctcttgcga
    27961 catgagaatc cagaatttga ggtgaaagat gattttattt tatgagtatc caaaatgttc
    28021 gacttgccga gcagcaaagg cggagctgaa gagtttggga ttggaatttg aagctattga
    28081 cattaagtca acaccgccta gtgctgaaga gataaaagct tggatggagg cgacgggact
    28141 agaattgaaa aagtatttta atacttctgg gaatagctat cgtgaattag gcttgaaaga
    28201 taagtttgat agtcttagct tggagcaggc acttaatctt ctagctaatg atggtatgct
    28261 gattaagcgt cccctactga ttcaggatgg taaaatctta cagattggct atcgaacaaa
    28321 atacgaaaat ctcggtttat aagccgagat tttgtttatt atagtgcata ttctattgaa
    28381 atatattcat gatgtttttt caaaagatag agcatctgag gcttatcttc taagagtttt
    28441 tggataattt cgtgatggac attgtggaga ttgattttaa cgcgaatctt catgccttgt
    28501 ttgaaaatat ggatttcgac atcattgcta tcttccttta agtaaggctc caatagctga
    28561 tagatagaat catgtatctc agggaaatgg atcgttccgt tggcgagttc cttgaaagat
    28621 gccactaata agttccaagg ttccttaaat gaaatgattg ctagtgctag tgtcaaaaag
    28681 aagtcgccag tataatgcag gaagccgaac caagagttga taggaacaag atagagcaga
    28741 agaattgcaa ttccaattgc acctgaaatg agtccgtcta caaggttgcc ttttgcttct
    28801 gctaatatga tagtagacag atgattgact ttggcactca tatggcggtt gtataggtaa
    28861 agccccatac agacaaaaaa catggttatg gtataaggga gaacgggacc tgtagtcatt
    28921 tgatgaccga ttccatgaac aaagtaagca aaggctgtcg cgcttgtttc aagtacagca
    28981 aatacaagga gtagtagagt tgcgagagat ttcatgatag cgtagagagg ttctagaaag
    29041 tatagtcctt gaggaaaagt ctcggttttc cgatggctat ttttcgatat gtaaactgca
    29101 actaaggacg aaataaatgc tattagagaa aagactccgt ctaggaggag ggcgtttagg
    29161 tcagtcatga tataaacggc aatacctgct agtccagata aaccgttcac aacagaggaa
    29221 acagttagag atttgcgttc gataattttg tggtccataa tccctccttt taatatatat
    29281 ctatatattt attttaccac aaaatgacac cgttttcaaa ttgagcggat taaaacaagg
    29341 tagagtgata agaaataaag gctcaaaacc cttgcaatag atctacgggt tttaagcctt
    29401 taatatctgt tatctgataa ttgaaatcag gttaatttcc actgttagaa tgaaatctcc
    29461 atcacaattg gtgtatggtc ctgacgagca ccggagtcaa tcatatctga cttacttact
    29521 ttgtcggcga tgcggtcgct gacaagccag taatcaattc tccagcctgt attgttgatt
    29581 ttactggtgc ggctacgttg tgcccaccaa gtataggcgt tgagaacatc accgtgcaag
    29641 tggcggaagg tatcagtaaa acctttagct agaagatttg taaaaccttg acgttcttca
    29701 tctgtaaatc ctggagattg acggttactt gcgggattag caaggtcaat ttccttgtga
    29761 gcaacgttgt agtcacctgt tgcaagaaca ggtttgtggc tatcaagttt tgctaagtag
    29821 tcagcatact gtacatccca gatttgacgg tcagcaagcc gtttcaagcc atcaccagca
    29881 tttggcgtat aaacttgtgt gacatagaag tcatcaaatt ctagcgtgat gatacgccct
    29941 tcagaatcca tggttgttgg agcaccaatc tcaggaaagg ttatggttgg agttagatgg
    30001 tttttgtaga ggaagagtgt accagcatag cctttgcgag ctggttctac agatgaacgc
    30061 caggtattgg catatccagg gaagtagctt tccaaaattt ctagatgctt tttagtcggt
    30121 cccttatctg acaattttgt ctcttgaatg gcgataatat cagcgtcttc tgtcacaagt
    30181 gtatcgatga ctgcgcgtga taatagggcg cgaggagact ctgcagtcaa tgcagcgtta
    30241 agagaatcaa tgttccaaga aatgagtttc ataattttcc ttttctagtc tgttactgtt
    30301 ttacattata ccaaaaataa aagttgcagg cgaatatgga aacttgaaaa aaattttata
    30361 attcttgtat tataacatta aaattgtgat acaatagaac taataaatct gtgtcaagga
    30421 ggttattatg aaaagaaaat tatttgtttt tgcctggtta gccctagttt tggaaatgct
    30481 aatctactac tggtaccagt tgcctgcttt aaatattttg agtgtggact tctggatatt
    30541 tttccttcaa tctgtcggat tggtctggct catactttcc acgttcagct ccaaaggtgc
    30601 tttttaaaag gtgaccaaga tttccggtcg ccagagacaa gtagatacct atcagattaa
    30661 aacctcgcaa ctaccagctt acttaaaatg gttgggacga atttgggtat tggttgtcct
    30721 gggtttgttg gggttggggc ttatcaactc acgtgtattt cgtgctaagg attacgctgc
    30781 tgtcataagc gtcaaggatg cggattttaa agcagatttt cctgaaacag acatatccaa
    30841 attggccctc ctagatcgcg cctcagccga aaaaatcggt gatacttatc taggtaccat
    30901 tgacaaggtc tcccagtttg gcatttcgga tgattatcgc cagattacta tcggtcagca
    30961 accgttccgt gtatcgcctt tggaatataa gaatttttgg aaatggctga gcaaccatca
    31021 agatgggatt ggttactacg tgaaggttaa tcagacaaca gggaaggcag aacttgccaa
    31081 gttgagcaaa gccatgcact attcagattc cgaatttttg ctgaatgata ccttacgtca
    31141 tcttcgcctg caatacccga caactatttt tgggaaacca tccttcgaag tggatgacca
    31201 aggaaatcca tattatattg caacgattta cgagccaaaa tttggacttt catcaaatga
    31261 tccgattggt gctattgtat tggatgcagt aacaggcgaa agtaagaagt atggtctgga
    31321 agatattcca gagtgggtag accgtgttta ttcggcaaac aatgttatca gccgtgtaga
    31381 tgaccactat acttatcaaa atggtttctg gaataccatt ttcagtcaaa ctggtgtgaa
    31441 aaatacaaca gacagctata attatatttc tatcggctca gatatttatc tctacacagg
    31501 tatcacctca gcaactgctg attcatccaa ccttggattt attcttgtca atatgagaac
    31561 tcgtgaaatt accaactaca aattggcttc ggcgactgaa acagcagcgc agaagtctgc
    31621 agaaggtgaa gtgcaggaaa aaggctataa agccactaat ccaagcctag ttaagttggc
    31681 agatacagcc tactatttag tatccttgaa agatgatgcc ggcttggtta aatcatacgc
    31741 tcttgttgat gccgaagatt atcaacaggt tactgttaac aacgatattg cgctcttaat
    31801 ttctcaagtc acaggaacgg atgcttctag cctttcagga ttgacggtga ctgaaaatgg
    31861 agaagttggt gaacagactg aggttatcag tggaaaagta gaagctttag cgagtcaaat
    31921 aattggcggt gcgacaatct actatattga gtctgagggc aagatttaca aagtcaaagc
    31981 aacggaagat agtacagatc gattaccatt tatcaaagta ggagatcact ttagcggtca
    32041 gttagataag aaaaattatc ttaaaaactt taaggttaat ccagaataat cataagaaag
    32101 agtgaggtag aaaagccttg ctcttttgct tgtcgtcacc catgcgaaat atttcaactt
    32161 atggtataat ggagtgttgt ggttctttga caggaatccg tagattattc tgtcgagtat
    32221 ccatattctt attctgaagg agtattttgt gagtttattt agaaaaaaac aagcagtcgg
    32281 tcgccatagt caaatgcgtc ggcatttagg cttagttgat ttgatatttt taggtatcgg
    32341 ttcgatggtt ggaacaggta tctttacggt aactggtttg gcagcagccc agtatgcagg
    32401 gccagctctg atcatctcaa ttgttattgc agcaatttca gtcggactaa cagccttgtt
    32461 ttacgcagaa tttgcctcgc gtataccgac taacggtggt gcctatggtt atctctactc
    32521 ggtttttggt gaatttcctg cctggattgc tggctggctg acaatcatgg aattcttgac
    32581 tgctgtatcg agtgttgcat ctggttgggg agcttatttg aaaggactcc tggctaattt
    32641 tggtatcgct atgccgacag ctctgaatgg aacttttaac cccgctgcag gtacctatgt
    32701 agacctctta ccagttctcg tcttgatttt tgtggtagga gtggtcttgc ttaattctaa
    32761 ggcagctctt cgtttcaatt cagccttggt agttttgaaa ttttcagcgt tggctttatt
    32821 tattttagta gggttattct ttatcaaacc agaaaactgg tcgaacttct ctccttttgg
    32881 ttttggtgcc atttatggtg gacaagcagg aattatggca ggagcgtctc tcatgttctt
    32941 tgccttcctg ggatttgagt ctatttcatt agcaattgat gaggtgaaaa aacctgagaa
    33001 gaatgtacca aagggaatcg tgttgtctct atccattgta acaattcttt atattgtagt
    33061 gacattagta ttgactggta tggttcacta tactaagttg aatgtggcag atgcggtagc
    33121 ttttgcactt cgagaagttg gattggattg ggcagcgagt tatatttcta ttgtagcaat
    33181 actaacattg attacagtat gtatttcgat gacctacgcc ttgtcgagaa tggtctattc
    33241 catcagtcgt gatggtttgc taccgaaatc attgagccga ttgacacaaa caagcaaggt
    33301 tcctaagaat gcgaccattc ttgtggggat ttttgcagcc atttgtgcag gtatttttcc
    33361 actagcaagt attgcttcct tcctcaatat ctgtacctta gcttatttga tcatgctggc
    33421 gttgggtatt attcgtctgc gtcaagtcga aggtttgcca aaagatggac aatttaaaac
    33481 acctcttgtt cccttgttac ctatcctctc gattatcatt tgtctttcat ttatgttcca
    33541 gtatagttta gatacttggt tagcattcgg agtatccttg gttattggaa ttttgattta
    33601 cttctgttat ggctatcgtc attcggaagc aaaataatag a
//
LOCUS       DBNFCK010000006        32552 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.006, whole genome shotgun sequence.
ACCESSION   DBNFCK010000006 DBNFCK010000000
VERSION     DBNFCK010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 32552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32552
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.006"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            57..896
                     /gene="licT"
                     /locus_tag="AC1PIW_000254"
     CDS             57..896
                     /gene="licT"
                     /locus_tag="AC1PIW_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HIP6605223.1"
                     /translation="MIIKRVLNHNAVIAQNNKNVDILLFGKGIAFGKKVGDAIPPSSI
                     EKSFLLKNSDNMNRFTELFIDVPIELVYVCEKIINLGKITLGNHFDEIIYINLTDHIN
                     SSIERHKEGVVVTNPLRWEIAKYYKEEFELGKKALSIIKKDLHVELPVDEAAFIALHF
                     VNANLENIFQESYRITGIIMKIEQIIQDYYSTEFNQDSIDYYRFITHVKLFAHRLVEG
                     NEYHDEDDVDLLELMKKKYPREYQCGTRVADFIRLEYDYLLSPSELVYLIAHIRRLTK
                     NLS"
     gene            907..2748
                     /locus_tag="AC1PIW_000255"
     CDS             907..2748
                     /locus_tag="AC1PIW_000255"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HIP6605224.1"
                     /translation="MKYKNTALAILGAVGGEKNVLRATHCVTRLRLELKDENIVSDER
                     VKSISGVIGIMKKNGQYQIILGNDVANYYKEFTALGKFDSDSVQQLKKANVLEQVIEY
                     IAGSMTPLIPAMLGGGMIKVLVIVLPMLGLLKADSQSISFLAFFGDAPYHFMPIFLAY
                     SASQKLKVTPALAMSVAGILLHPNFVQMVSSGDPLHFLGAPVTPASYGSSVIPILIMV
                     WLMKYIEAVFNKVTPAVTKSFLQPTLVLLVSGFIALVLVGPLGVIVGEGLSQLVEQMH
                     GVAGWLTLAVLGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     IAVAFKSKNSNTKQIAFAAGFSALFAGVTEPALYGVTLKYKKPLYAAMIGGGLAGLFV
                     GLTGVKAYLFAVPSLIALPQFIYSEAASNITNAMIAAAISIIVTFILAYFLGIDEETS
                     TVNLEKVAPGISSRKNVFSPLSGQILPLEKVNDATFSKKMLGEGVAIIPKDGKVYAPF
                     DGTVTSLFPTKHAIGLTSDEGVELLIHFGLETVELKGRGFVSHVSDGEKVEKGQLMLE
                     VDVEMLVAEGYDIVTPVVVTNTQEYLDVLLLSTKEEVNYADDLLAVL"
     gene            2763..4187
                     /locus_tag="AC1PIW_000256"
     CDS             2763..4187
                     /locus_tag="AC1PIW_000256"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HIP6605225.1"
                     /translation="MSIFPDNFLWGGAVAANQVEGAYNEGGKGLSVQDVLPNGGLGEA
                     TQEPTEDNLKLQAIDFYHRYKEDIALFSELGFKVFRTSIAWSRIFPNGDEEFPNEEGL
                     QFYDELFDELLKNGIEPLVTLSHYETPLYLARKYHGWVDRKMIQFFEKFARTVFERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDLPNEELSKQDLYQAIHHELVASSLVTKIAHEI
                     IPDCKVGCMVLAMPTYPMTPNPEDVWAAHAYENLNYLFSDIHVRGYYPNYAKRYFKEN
                     GIKITFVEGDEELLKNNTVDFLSFSYYMSITQAADPSKYSSGKGNILGGLVNPYLEAS
                     DWGWQIDPIGLRLTLNRYYNRYQIPLFIVENGLGAKDQLVKDEAGGLTVHDDYRIQYM
                     QEHLLQVAEAIQDGVEVMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGSGSLTRY
                     RKKSFYWYKEVIQSNGSILSNSSK"
     gene            4944..5945
                     /gene="trpX"
                     /locus_tag="AC1PIW_000257"
     CDS             4944..5945
                     /gene="trpX"
                     /locus_tag="AC1PIW_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HIP6605226.1"
                     /translation="MLKNKNLLATIVALTVMVVAALFMTQKEQNNSSTSTEKVKIGVL
                     QFVTHDSLDEIYKGIKAGLEEGGYTTTDNLEIDFMNAEGDQSQVQTMSKKLVDNGNEL
                     LIGIATPAAQGLANATTELPIIMGAVTDPVGANLVTDLKNPGGNITGVSDQTPVADAV
                     SLIKEITPDAKTIGVLYSSNEDNSKIQVAEFKAAAEEAGYTVLEYAVASSNEIAATVE
                     VASSKADVLFTPVDNTVASAFSTVVSVANKTKTPIFTSVEDMVEGGGIASVTLSQYDL
                     GVATGKMAAKILDGANPADTPVQIFNEGTVVVNQKVAKELGITLSDDVISKASKVIE"
     gene            5968..6834
                     /locus_tag="AC1PIW_000258"
     CDS             5968..6834
                     /locus_tag="AC1PIW_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002902146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6605227.1"
                     /translation="MIVSTVSQALLWAVLGLGIFVTFRILNFPDMTTEGSFPLGGAVA
                     VTLLTQGVHPILATLAAVLVGCLAGFVTGLLYTKGKIPTLLAGILVMSSCHSVILFIM
                     GRANLGLLNTKRLQDFLPFSEQVNGLLLGLGSLAVVIGALMFFLYTRLGQAFIATGDN
                     PDMARSFGINTSRMELLGLVLSNGIIALAGALIAQQEGYADVSRGIGVIVVGMASLII
                     GEVIYGNLTMLERFLAIVVGAIFYQFLILVVISLGINTSYLRIFSAMILATCLMVPEL
                     KNKLLKGASLSR"
     gene            6831..7589
                     /locus_tag="AC1PIW_000259"
     CDS             6831..7589
                     /locus_tag="AC1PIW_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6605228.1"
                     /translation="MKAIVELKNATKVVTNGFDEERVILDNVNLTIYQGDFITILGGN
                     GAGKSTLFNVIAGTLPLTSGSVHILGEDVTNWPAEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKYRGEGRGLLPRRLAQQTEEFAQLLPRVGNGLEKHLDTPAGLLSGGQRQAL
                     SLLMASLKQPELLLLDEHTAALDPKTSQSLMQLTDELIETKGMTALMVTHQMEDALRH
                     GNRLIVMKNGQIIQDQTAEEKSKMKLEDYYKLFD"
     gene            7772..8257
                     /locus_tag="AC1PIW_000260"
     CDS             7772..8257
                     /locus_tag="AC1PIW_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HIP6605229.1"
                     /translation="MDKLVFESLADLGMDYTIVEHPPALTTEEADRYIEGVEGVRTKS
                     MFLTNKKKTAFYLLIMDDQKQLDMDQFRDLVGANRIRMASSDSLMEKMHLPAGAVSVF
                     GLLHNVDKDIQVFFDKEILSEPILTFHPNVNTKTIFVKTEDVLRFVEEIGFSATIVDW
                     G"
     gene            8263..8667
                     /locus_tag="AC1PIW_000261"
     CDS             8263..8667
                     /locus_tag="AC1PIW_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HIP6605230.1"
                     /translation="MRKCLAICSLLCCLFLVGCRTGAAYQAEIEPMEQSELYTEKEIE
                     AAILVVQDYFDHHFNSCKLLTIGYSGDNEKEFDEWAEHYGAEEAIILTSSFKVAAEGA
                     EPTLEPNSTHTDWKWILVRNVGGKWEHKGHGY"
     gene            9057..10718
                     /locus_tag="AC1PIW_000262"
     CDS             9057..10718
                     /locus_tag="AC1PIW_000262"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0004534 - 5'-3' RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HIP6605231.1"
                     /translation="MSTIKIMALGGVRENGKNLYIAEVNEHIFVLDAGAKYPENEQLG
                     VDVVVPNFDYLEENKHRVAGVFLSHGHADAIGALPYLLEKVKVPVFGSHLTIELAKLL
                     VKGNNATKKFNDFHVINAETEIDFGDSVVSFFQTTHSIPESLGIVVKTDEGNIVYTGD
                     FKFDQAADKFYRTDFGRLAEIGNEGVLALLSDSANADSNVQVASMHEAGEEILNTIAD
                     CDGRVIVAAVASNIVRIQQIFDAAEATGRRVVLTGHDVENIVRTAIQLKKLRLVSERL
                     LVKPKDMNKYEDHELIILETGRMGEPLNGLRKMSIGRHRYVEIKEGDLVYIVTTPSIS
                     KEAVVARVENLIYKAGGTVKSITKNLRVSGHGNARDLQLMLNIMKPKYLFPVQGEYRQ
                     LDAHAKAALEIGMYPENIIIVKRGDVMAFEEGDFVHSGAVPAGDVMIDGNAMGDVGNI
                     VLRDRKILSEDGIFIVAITVNRREKKIISKAKVNTRGFVYVKKSKDILREASELVNTT
                     VENYFTKDSFDWAELKSAVRDDLAKFLFDQTKRRPAILPVIMEVK"
     gene            10783..11559
                     /locus_tag="AC1PIW_000263"
     CDS             10783..11559
                     /locus_tag="AC1PIW_000263"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIP6605232.1"
                     /translation="MAIMKIEYHSEALDMSRQVTVLYPDRNRVENPDDNDIPVLYLLH
                     GMGGNQDSWLNRSTIERLVRYTNLIVVMVNTDKAWYTNTTYGMNYYDAIAIELPQILK
                     RFFPNMSDKREKNFIAGLSMGGYGTFKIAMMTNRFSHAASLSGALYFDFDNPAAAELG
                     SLSYWQGVFGDISDKQNPNNLLEIAKQSDKKTKFYAWCGEEDFLFEGHQKAVEELKEL
                     GFDIEASFGPGKHEWYYWNQQIEKVLAWLPIDFKLEERLS"
     gene            11625..12284
                     /locus_tag="AC1PIW_000264"
     CDS             11625..12284
                     /locus_tag="AC1PIW_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M57 family metalloprotease"
                     /protein_id="HIP6605233.1"
                     /translation="MKLIGNILRWIWRTFWGLIWLSMVLLACGLGLLFYFQQEAAPQM
                     LQTLPQEVQLMVKGQSEVTTDTVNTADHGRWESNTATVYIETQNPTFVAAYETAIANW
                     NATGAFTFVMTSDPNQADIIATEMNDGNTQAAGEANSTTNLLTNYYSSVTVCLNSFYL
                     LNDQYGYDMDRIIHTAEHELGHAIGLDHEDSQTSVMESAGSNHGIQQADIDAVLALYS
                     E"
     gene            12295..12660
                     /locus_tag="AC1PIW_000265"
     CDS             12295..12660
                     /locus_tag="AC1PIW_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605234.1"
                     /translation="MLRTITNTIKRYPEQALLFLYNAGIFAWMQSTSHSIMEQIGIDS
                     NWFDKIPEPIKAWTGASLESMQTLLNSSAWGWLIVSMILMLVIRFVKGLIKFVIMLII
                     IGGGLYLLWQNKELLSGLV"
     gene            12761..14659
                     /locus_tag="AC1PIW_000266"
     CDS             12761..14659
                     /locus_tag="AC1PIW_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIP6605235.1"
                     /translation="MIILSGNKIERSFAGEVLFNNINIQVDERDRIALVGKNGAGKST
                     LLKILVGEEAATSGEISTKRDLSLSYLAQDSCFESENTIYDEMLHVFDDLRMTEKRLR
                     SMEEQMGSLSGNELDQLMKTYDSLSEEFRLAGGFSYEADIRAILNGFKFDQTMWDMKI
                     SELSGGQNTRLALAKMLLESPELLVLDEPTNHLDIDTIAWLENYLVHYKGALIIVSHD
                     RYFLDKVATLTLDLTKHSLDRYVGNYSQFVELKEQKLQTELQNYEKQQKEIAKLEDFV
                     QKNIVRASTTKRAQARRKQLEKMDRLDKPTTGQKSANMTFQSDKTSGNIVLTVENAAV
                     GYDGNILSQPISIDQRKLDAIAIVGPNGIGKTTLLKSIVGALPFIKGEAWLGANVEVG
                     YYDQTQSALTPSNTVLEELWSAFPTTPEVEIRNRLGAFLFSGDDVKKSVSMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     KVLEISETGSTLYLGDYDYYLEKKAELEAEAQPDQLESTSQSAGAMDYQAQKENQKEQ
                     RKLARRIEQIEAEIDSIENRLNELNQAMLETNDIGQLTDYQKEIDQLTSQQESLMEEW
                     EELSEQMG"
     gene            15097..15573
                     /locus_tag="AC1PIW_000267"
     CDS             15097..15573
                     /locus_tag="AC1PIW_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIP6605236.1"
                     /translation="MNFGQQIKDLRKKKGLTQEQFALKLNVTRQAVSNWENDKNLPDL
                     ELLILMSSVFSISLDQLILGGTDMNNMTEKLVKDGREGRRTQMHLTITIIGSFLMLLG
                     FVCFVIKANSVEYIDAEGILHENFYLIPVGYLLVFTGALATLLSGLALHRFRKEHK"
     gene            15575..16402
                     /locus_tag="AC1PIW_000268"
     CDS             15575..16402
                     /locus_tag="AC1PIW_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative RNA methyltransferase"
                     /protein_id="HIP6605237.1"
                     /translation="MTKHQRFANSDQFFACPHCGQALGLDLNSLRCPNRHTFDIAKQG
                     YVNLAPQVKQSANYHKSSFENRQAFLEAGYYDHLYEALEEKIAELGLRSVLDIGCGEG
                     FYSRKLAEKMDLDILAFDISKDSILLAARTDRTKSVKWFVGDLTKLPIQDKTIDGILD
                     IFSPANYQEFARVLKAGGAILKLVPGPNHLKELRHLAKDQLRKESYDNQDIVDHFKSY
                     MGQVEQEVVSRTLPIGIEQARILADMTPLFFQVDQSKLDLSQLTEITVEGLLLIGSI"
     gene            complement(16542..17330)
                     /gene="gatD"
                     /locus_tag="AC1PIW_000269"
     CDS             complement(16542..17330)
                     /gene="gatD"
                     /locus_tag="AC1PIW_000269"
                     /EC_number="6.3.5.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0140282 - carbon-nitrogen ligase activity
                     on lipid II [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit GatD"
                     /protein_id="HIP6605238.1"
                     /translation="MVYTSLKSPEKDYTYDLHIAHLYGDLMNTYGDNGNILMLKYVAE
                     KLGARVQVDIVSLTDEFDKNFYDIAFFGGGQDYEQSILAKDLPTKKDSLADFIENDGV
                     MLAICGGFQLLGQYYIEAGGRKIDGLGIMGHYTLNQTNNRYIGDIKIHNDEFNETYYG
                     FENHQGRTFLADNQKPLGKVVYGNGNNKEDGGEGLHYKNTFGSYFHGPILSRNANLAY
                     RLVTTALRKKYGQDIQLASYADILSKEVAEEYGDVKSKAEFDTQ"
     gene            complement(17330..18673)
                     /gene="murT"
                     /locus_tag="AC1PIW_000270"
     CDS             complement(17330..18673)
                     /gene="murT"
                     /locus_tag="AC1PIW_000270"
                     /EC_number="6.3.5.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_function: GO:0140282 - carbon-nitrogen ligase activity
                     on lipid II [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit MurT"
                     /protein_id="HIP6605239.1"
                     /translation="MKINTALGILAGKSSQFILKKLGRGTTLPGKIALKFDKHILDSL
                     AKDYEVVVVTGTNGKTLTTALTVGILQEAFGEITTNTSGANMITGITATFLSAKKNKN
                     GKKIAVLEIDEASLARVTDFIKPSLIVFTNIFRDQMDRYGEIYTTYQMILDGAAKAPQ
                     ATILANGDSPLFNSTNVINPVKYYGFATEEHEPRLAHYNTEGVLCPHCHQIIQYKLNT
                     YANLGSYICTNCGFSRPELDYKLTKLKEITNTSSTFVIDGQDYKINIGGLYNIYNALA
                     AVSVAEFFGVSPEKIKAGFDKSKAVFGRQETFKLGDKDCTLVLIKNPVGATQAIEMMK
                     LAPYDFSLSVLLNANYADGIDTSWIWDADFEQITQMDIPQVFAGGVRSSEIARRLRVT
                     GYPENQITETPKLEDIMTLIEQSSSQHAYILATYTAMLEFRDLLAQRQAVGKEMK"
     gene            18855..19706
                     /gene="cdaA"
                     /locus_tag="AC1PIW_000271"
     CDS             18855..19706
                     /gene="cdaA"
                     /locus_tag="AC1PIW_000271"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HIP6605240.1"
                     /translation="MLNLNQLLDTGYWSSLIASPWTALLHLIDISIVVYLIYNFSKSI
                     AGTKIMTLIRGVFLFIIVQIIASLFGLQTIAWLINQVITYGVIAAVVIFAPELRAMLE
                     KLGRTTQIFGTNTVSAEEKLIVAFLKSVAYMSPRKIGALVAVEQAQTLQEYRATGIPL
                     NADISQELLINIFIPNTPLHDGAVIVKEDKVAVACAYLPLSESAGISKEFGTRHRAAI
                     GLSEVSDAFVFIVSEETGSISIARNGIFKHDLTLDEFEAELRATFISENVEKKSIWKR
                     LGGGKHE"
     gene            19699..20403
                     /locus_tag="AC1PIW_000272"
     CDS             19699..20403
                     /locus_tag="AC1PIW_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HIP6605241.1"
                     /translation="MNDKFKAVGHLALSIFLALLLFFYATTTNYKNSESAAQNTESET
                     YVHTLSNVPIEIEYDTNKYFISGFSSTVAVELRGSNRVLLQRESDESTRTFQVTADLR
                     ELSDGTQTVKLQLTNLPAGVSATLAPDAITVKIGKKVSKAFPVAGRVYSNQLAEGYSL
                     SKVSVNVDTVKVTTDEETMAKIDRVEAVAMDVSNLSENYSGTAKLQAVDSDGNVLPVV
                     LSQTEANMQIILTKTK"
     gene            20416..21765
                     /gene="glmM"
                     /locus_tag="AC1PIW_000273"
     CDS             20416..21765
                     /gene="glmM"
                     /locus_tag="AC1PIW_000273"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIP6605242.1"
                     /translation="MGKYFGTDGVRGEANVELTPELAFKLGRFGGYVLSQHETDVPRV
                     FVARDTRISGQMLEAALIAGLLSVGIHVYKLGVLATPGVAHLVKTEKASAGVMISASH
                     NPAQDNGIKFFAGDGFKLDDALEAEIEALLDAEEDTLPRPSAQGLGDVVEYPEGLRKY
                     QQFLVSTGTDLDGMKVALDTANGAAATSARQIFVDLGADLTVMAEKPDGLNINEGVGS
                     THPEKLQELVKETGSQIGLAFDGDSDRLIAVDENGVLVDGDRIMYIVGKYLADRGLLA
                     KNTIVTTVMSNLGFHKALDREGIEKAVTAVGDRYVVEEMRKEGYNVGGEQSGHVILMD
                     YNTTGDGQLTAVQLTKIMKETGKKLSELAAEVTIYPQKLVNIRVENSMKDKAMEVPAI
                     AAIIEKMEAEMAGNGRILVRPSGTEPLLRVMAEAPTDAEVDYYVDTIADVVRAEIGS"
     gene            21929..23473
                     /locus_tag="AC1PIW_000274"
     CDS             21929..23473
                     /locus_tag="AC1PIW_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIP6605243.1"
                     /translation="MSILEVKNLSHGFGDRAIFENVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGQLQPDEGKVEWSRYVTAGYLDQHAKLEKGQSVRDVLRTAFDELFKTEARIN
                     DIYMSMAEEGADMDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDYGMDKDVT
                     ELSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQLLTRYTGDYYQFQEVHAMKRAQLEAAYERQQKEIADLQDFVN
                     RNKARVATRNMAMSRQKKLDKMEIIELQAEKPKPSFDFKMARTPSRFIFQTTDLEIGY
                     DRVLTRKPLNLTFERNQKIAIVGANGIGKSTLLKSLLGIIPPLGGSVERGEYLELGYF
                     EQEVAGGNRQTPLEVVWDAFPALNQAEVRAALARCGLTSKHIESQIQVLSGGEQAKVR
                     FCLLMNRENNVLVLDEPTNHLDVDAKDELKRALQAYKGSILMVCHEPDFYEGWVDDVW
                     DFNDLT"
     gene            23604..24380
                     /locus_tag="AC1PIW_000275"
     CDS             23604..24380
                     /locus_tag="AC1PIW_000275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIP6605244.1"
                     /translation="MTDYVNYNQERWNRVSARQGNAYTVPLSHEEFLVAKDKPLSVSL
                     TVGKTVPLDWFEKAKGKKLLGLACGGGQQGPISAAHSYETTILDFSKEQLDKDRLVAE
                     RENLDLQTVQADMTQAFPFEDESFDIIFCPVSNIYIEDLANMWQESYRVLKKGGLLMV
                     GYMNPWIYVFDADDVWEHPDKTLVPKYSLPFNARQLEEAGQITIDPEYGYEFSHTLEE
                     QIAGQLRAGFAMIDFYESKDSRNRLTQFASDYLANLSIKW"
     gene            24558..24755
                     /locus_tag="AC1PIW_000276"
     CDS             24558..24755
                     /locus_tag="AC1PIW_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605245.1"
                     /translation="MKTKLLLGVALLSVGLSVAPPTIVEVEAQTTHVSDPALRNSYPY
                     NLSHYLEVNVGSGQTKKVQLF"
     gene            24791..24928
                     /locus_tag="AC1PIW_000277"
     CDS             24791..24928
                     /locus_tag="AC1PIW_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605246.1"
                     /translation="MADDGTGRVTSQTWSGNAVWGDASPYYELSEIKRTNPFNPFIQM
                     P"
     gene            24968..25336
                     /locus_tag="AC1PIW_000278"
     CDS             24968..25336
                     /locus_tag="AC1PIW_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605247.1"
                     /translation="MKITTPSRKTQTANTANARLGIYRNTYDLVGRQFLMVSNSINLY
                     GRWEYEYGTITEVRYDGLLQTNITGVKGQSGSPVVVDGRIIGVVSNIDSNSKIVITPL
                     TTEMKTQLFDKNGIKNIDIY"
     gene            25707..26912
                     /gene="sstT"
                     /locus_tag="AC1PIW_000279"
     CDS             25707..26912
                     /gene="sstT"
                     /locus_tag="AC1PIW_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HIP6605248.1"
                     /translation="MNKIISVWKKMNLIRKIGIGVVLGVLLGLIAPKITVIALFGSLF
                     VGALKAIAPLLVLTLVAHALSQAPAGQKSNMRTVICLYLFGTFAAAFIAVGASYLFPI
                     KLVLSTTTTTDITPPQGIAEVFQDLLLKVVDNPINALATANYIGVLTWAAVFGLAFRH
                     ASKTTKDLLQSTAEVISKVVGWIIGLAPFGIMGLVFDTIANNGLTALKDYGLLLLLLV
                     GSMIFVALVVNPLIAFLVMKKNPYPLVFECLRVSGVTAFFTRSSAANIPVNMQLCKRL
                     GVDPDTYSVSIPLGATINMAGAAITINILTMAAVHTLGISVDFSSALLLSVVASLSAA
                     GASGVAGGSLLLIPVACSLFGIPNELAMQVVGVGFVVGVIQDSCETALNSSTDVLFTV
                     VAERSAWKK"
     gene            27013..28413
                     /gene="pepV"
                     /locus_tag="AC1PIW_000280"
     CDS             27013..28413
                     /gene="pepV"
                     /locus_tag="AC1PIW_000280"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HIP6605249.1"
                     /translation="MTVNFRAEFDKRKDEFLADLFDLLRINSERDDSQADAQHPFGPG
                     PVRALDKFLEIAQRDGYPTKNVDNYAGHFEFGEGDEVLGIFGHLDVVPAGSGWNTDPY
                     EPQIIDGKLFARGSSDDKGPTMACYYGLKIIKELGLSTSKKVRFIVGTDEESGWADMD
                     YYFEHVGLPLPDFGFSPDAEFPIINGEKGNITAYLHFAGENSGAAKLHSFTGGLRENM
                     VPESATAIISGDLADLDSKLVDFTAAYGIKADAETLENGQVQVTVIGKSAHGSTPEEG
                     VNGATYLAKFLSQFAFDEAAKAYLDLAGQVLLEDHDAKKLGVAIYDEQMGALSMNAGV
                     FKFDETSSDNTIALNFRYPKNTNPETIKAGLEKLGVEAVSLSEHGHTPHYCPIDDPMV
                     ATLLSVYEKHTGLKGHEQVIGGGTFGRLLKRGVAYGAMFPGDVNTMHQANEFIEVEQL
                     YRAAAIYAEAIYELIK"
     gene            28454..28912
                     /locus_tag="AC1PIW_000281"
     CDS             28454..28912
                     /locus_tag="AC1PIW_000281"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6605250.1"
                     /translation="MELKELYFSDMEMVKELFLSVFSQEPWNDDWSDEEQLDCYIGDL
                     LGHSRALCFGLFDQEKLIGLSLGYIRYWYEGTEYRIEEFCISRDYQGQGHGASLLADI
                     EKQLADRKIVHILLQTERNLLAYSFYKKCVFRALEEDVTIVKKVGHHGTC"
     gene            28899..29477
                     /locus_tag="AC1PIW_000282"
     CDS             28899..29477
                     /locus_tag="AC1PIW_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HIP6605251.1"
                     /translation="MELADIRQAIIEDVANRDFTNKGIAPLFQAPSTAKILIIGQAPG
                     LKTQEKGRLFDDASGSNLRQWLGVDRQTFYESGHFAILPMDFYYPGKGKSGDLPPRKD
                     FAPKWHPLIMKQLPQIELTILVGNYAQTYYLEKSQRNLTERVKHADDYLPTYFPLPHP
                     SPRNNIWQAKHPWFVEQTLPILKNNINILLGK"
     gene            29549..30325
                     /locus_tag="AC1PIW_000283"
     CDS             29549..30325
                     /locus_tag="AC1PIW_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HIP6605252.1"
                     /translation="MRKWKKIIIGCFAILTVCLLFAPTLARANQIPDRPTGTTVVDET
                     QLLSSETIAEIDQLNRSWASTEQALQVGVYVTESLSSDIESLANETFRHWQVGFARTD
                     NGVLLVVAIADRAFRIETSDNAATVLTDVEAKDILENAREFFRQEDYSRGISYIVNSI
                     GDRFYGTSIGKNQLAAIEEGTSEKDDGFWLFLIVILIAILFGIIDKSSRGGGGPGNLL
                     WMLVDDHHHYHNHHSSNSSSSSFGGGSWSGGGGGGASSGW"
     gene            30398..30979
                     /locus_tag="AC1PIW_000284"
     CDS             30398..30979
                     /locus_tag="AC1PIW_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIP6605253.1"
                     /translation="MKKRWLVILIPVLALLFLGMGAVGQYNGLVDSYAEVENAQANVD
                     TQLQRRYDLIPNVVAAVKGAMEHEEEIFTAIAEARAKIGSSQVGTSDYNQAQSQLDSA
                     VSRLLVVAENYPQLTANQQVSDLITELEGTENRILVARKDYNAVATTYNKKIKRFPTS
                     IYAGLFGYEKVELFQATNDAATTVPSVDLSDKE"
     gene            30991..32460
                     /locus_tag="AC1PIW_000285"
     CDS             30991..32460
                     /locus_tag="AC1PIW_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0016600 - flotillin complex [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HIP6605254.1"
                     /translation="MDFGFDILAIMIPIIVVVLFVLLFVFGYVSAKPNEAIVITGLGK
                     PRTLIGRSGFMIPFIEKRSYISIEQFSTDVQTTDFVPTLDFINVKADAVVKVKVGVSD
                     ELLNAAAQNFLNWKTADISASIQDVLEGNLREIIGQMELRDMVNNRQAFAEKVQSNAA
                     PDLAKMGLEIIAFTVQSFTDDNDVIKNLGIDNIVTIQKDAANARAKAEREQAEVRARE
                     DKAANDARVAADLEIAKKQNELAIEQANLKRRSDVQLAQANAAYGIEEQAQRKEIERA
                     TAEANIVKQQKEAEVKAEEVKVREQELSATIRKQAEAEKYARQQAAEADLIERQRKAE
                     AELYETQREAEAQKARAEAAKYAAEQEAAGIEAKGRAEAEAIRLKLEAEAEGLSKKAD
                     AMAKFNDAAVTEMVVNVLPEIAKNIAAPLGNVDKITMYGEGNASKMVGDLMTTMDKTT
                     EGLGLNVRDLISATLTGRAMSTGLLTAQETKEVEGDKEK"
BASE COUNT         9431 a         6198 c         7643 g         9280 t
ORIGIN      
        1 atatatgtcg atttctttta agtaggttct tttattttaa gtttaaagga aaatttatga
       61 tcataaagcg tgtattaaat cataatgcag tcatagcaca aaataataag aacgttgata
      121 tcttgttgtt tggtaaagga attgcatttg gaaagaaggt cggagatgcg atcccacctt
      181 cttcgataga aaaaagtttt ttgctcaaga atagcgataa tatgaatcgc tttacggaac
      241 tatttatcga tgttcctatt gagttggttt atgtttgtga gaaaataata aatttaggaa
      301 agataacact tggaaatcat tttgatgaga ttatttacat caatttgaca gatcatatca
      361 attctagtat agaaagacat aaggaaggag tagtcgttac gaaccctctt agatgggaaa
      421 ttgccaaata ttataaagag gagtttgaac tggggaaaaa agcattatcc atcataaaaa
      481 aagatttaca tgtcgaactc ccagttgatg aagcagcctt catcgctttg cattttgtca
      541 atgcgaattt ggaaaatatt tttcaagaat cttatcgtat tactggaatt atcatgaaga
      601 ttgaacaaat tattcaagac tattattcta cagaattcaa tcaagattct attgattact
      661 accgattcat cacacatgtt aagttgtttg ctcatcgttt ggtagagggg aatgaatacc
      721 atgatgaaga tgatgtggat cttttagagt taatgaaaaa gaagtatcca agagagtacc
      781 aatgtggaac tcgagtagcg gactttattc ggttagaata tgattatcta ctgagtccaa
      841 gtgaattagt ctatttaatt gctcacattc ggcgtttaac aaaaaattta tcataaggag
      901 tctattatga agtacaagaa tactgcattg gcaatcctag gggcagtcgg aggagaaaag
      961 aatgttcttc gtgcaaccca ttgcgttact agattgcgac tggaactgaa agatgagaat
     1021 atcgtgtccg atgaacgtgt taaatccatt tccggagtca ttggcattat gaaaaaaaat
     1081 ggacaatatc aaatcatctt aggtaatgat gtagctaatt actataaaga atttactgca
     1141 ttaggaaaat ttgattctga ttcagttcaa cagcttaaaa aagcaaatgt cttagaacaa
     1201 gtcatcgagt atatcgctgg ctctatgaca ccacttattc cagctatgct tggtggaggt
     1261 atgataaaag ttctagtcat tgtcttaccg atgctggggc ttttaaaagc ggattcccaa
     1321 tcaatttcat ttttagcctt ttttggagat gcaccatatc actttatgcc gatttttttg
     1381 gcctactcag cttcacaaaa actcaaagtt acacctgctt tagctatgtc ggttgctggt
     1441 attttgctac atcctaattt tgttcaaatg gtatcttcag gtgatccact tcatttttta
     1501 ggtgcccctg ttacacctgc tagctatggt tcatcagtta ttccaattct tattatggtt
     1561 tggctaatga agtatattga agctgttttt aacaaggtca ctccagctgt aacgaaaagt
     1621 ttcttacagc caaccttggt cctgcttgtt tcaggtttca tagctttagt tcttgttgga
     1681 cctctcggcg ttattgttgg tgaaggatta tcgcaattgg ttgaacaaat gcatggtgta
     1741 gctggttggt taactttagc agttcttgga gcaatcatgc catttatcgt tatgaccggt
     1801 atgcactggg cttttgcacc gattttcttg gcagcttcaa tcgcgacacc tgatgtattg
     1861 attctaccag cgatgttagg ttctaacttg gcacaaggtg ccgcttctat cgcagttgca
     1921 tttaagagta aaaatagtaa cacaaaacaa atagcctttg cagctggatt ttcagcttta
     1981 tttgctggtg tcactgagcc tgctttatat ggtgttactc taaagtataa aaaaccattg
     2041 tatgcagcaa tgattggtgg tggtctagca ggattatttg ttggtttgac aggtgtaaaa
     2101 gcatacctct ttgcagtacc ttctctcatt gctttgccac aatttattta ctctgaagca
     2161 gcttcaaaca ttactaatgc tatgattgct gcggcaattt caattattgt cacatttatt
     2221 ttggcctatt tcctaggtat tgatgaagaa acgtcaacag ttaatttaga aaaagtagca
     2281 cctggcattt caagtagaaa aaatgtcttc tctcctcttt caggtcaaat tctcccttta
     2341 gaaaaagtaa atgatgcaac gttttcaaaa aaaatgcttg gagaaggagt tgcaattatc
     2401 cctaaggatg gaaaagtcta tgctccattt gacgggactg ttaccagtct ttttccaaca
     2461 aaacatgcaa tcggtttaac aagtgatgaa ggggtagagt tgcttatcca ctttggatta
     2521 gaaactgtcg agctaaaagg aagaggtttt gtaagccatg tttcagatgg ggaaaaagtt
     2581 gaaaagggtc aactgatgct ggaagtggat gtagaaatgc ttgtcgcaga agggtacgat
     2641 atagtgactc ctgtagttgt tacaaataca caggaatatc tggatgttct cttgctttcg
     2701 acaaaagagg aagtcaacta tgctgatgat ttattagcag tcttataatt cgaggagggt
     2761 atatgtcaat ttttccagat aatttcttat ggggaggagc agtagctgca aaccaagtag
     2821 aaggtgcata taatgaggga ggaaaaggat tatccgttca agatgttctc cccaatggtg
     2881 gactgggtga agctacccaa gaaccaacgg aggataattt gaaattacaa gctattgatt
     2941 tttaccatcg atacaaggaa gatatagccc tgttttcaga gttgggattt aaggtattta
     3001 gaacatcaat tgcttggagt agaatatttc cgaatggaga tgaagaattt ccaaatgaag
     3061 aagggctaca gttttatgat gaactttttg atgaacttct aaagaatggt atagagcctc
     3121 ttgtaactct ttctcactat gaaactcccc tgtatctggc aaggaaatat catggctggg
     3181 tggatagaaa aatgattcaa ttttttgaaa aatttgctcg tacagttttt gagcgataca
     3241 aagataaggt taagtactgg ttgacattta acgaggtcaa ttctgtctta gaattgcctt
     3301 ttacaagtgg tggaattgac ttgccgaatg aagaactttc aaaacaagat ttatatcagg
     3361 ccattcacca tgaacttgta gcttctagtc ttgttaccaa aattgctcat gaaattattc
     3421 ctgactgtaa ggttggctgt atggtcttag ctatgccgac ctatccaatg acacccaatc
     3481 ctgaagatgt ttgggcggct catgcctatg aaaatttgaa ctatttattt tcggatatac
     3541 atgttagagg atattatcct aattatgcca aaagatactt taaagaaaat ggcataaaga
     3601 ttactttcgt ggaaggtgat gaagagttat taaaaaataa tacggttgat tttctctcct
     3661 tcagttatta tatgagtatt actcaggcag cagatccatc aaaatattca tctggaaaag
     3721 gaaatatctt gggtggtttg gtcaatcctt atttagaagc ttctgattgg ggttggcaga
     3781 ttgatccaat tggtttaaga ttgacactaa atcgttatta taatcgatac cagattcctt
     3841 tattcattgt tgaaaatggt ttgggagcta aagatcagct agtcaaggat gaggctggtg
     3901 gtttgactgt tcatgacgat tatcgaattc aatacatgca agaacacctt ttacaagttg
     3961 cggaggcgat acaggatggc gtagaagtca tgggttatac ttcttggggc tgtattgatt
     4021 gtgtatctat gtctaccgca caattatcta aaagatatgg actaatttat gttgatagaa
     4081 atgatgatgg aagtggcagt ctaactagat atagaaagaa atcattttac tggtataaag
     4141 aagttattca atccaatggc agtatcctga gcaatagctc aaaatagact cttaaggtat
     4201 taaaaagttt tcagaaaatt taatcaaaag tattgacaaa gactgatgga taccgtataa
     4261 tcatctgtaa tcagaagaag tagtaaagca gaagtcttca gggagcctgt ggtgctgaga
     4321 acgggtggcg gagttttatg aaatgggctg atgtgcattt atgtctattt tatgacgtcg
     4381 ttagtcgtgc ttgccgtacc accagtactg ccttcacccg actgcctagt cctaaaatgc
     4441 cctaaattca caaggattgt ttggtatagc atactttgcg tagactgttt tggctgagta
     4501 cgaggcaggt cgacgcagct tatactggcg gtatagcaag gagagttaac gaagtaacta
     4561 gccaaaacag tcacgtaaat gaacttaaaa caatgatagt tcggtccgca caccttacag
     4621 tgcaactcct tgttagagga gatgagatat gggaggattc tagacaattc ccataagtga
     4681 ggtggcaccg tgtcattgac gccctcgcag gttgtttttt cctgcggggg cttttccatt
     4741 gtcttcgtag agtgctttta tattgcgtca cgttcggctt gcctaacttg agttatgtct
     4801 gcgcctccgt tccttatcta aaagcaatct actttgacaa ttattaacaa aacgaggtaa
     4861 tcatgatgaa acgatggcga tggtgggata atatattgat gacgtagaaa ttgtatattg
     4921 tgtattttac gaggagaatg aatatgttga aaaataaaaa tttacttgct acaattgttg
     4981 cccttactgt tatggtagtt gctgctttgt ttatgacaca gaaagagcag aacaattcat
     5041 ctacatcaac ggagaaagtt aagatagggg ttcttcagtt tgtaacccat gattcactag
     5101 atgagattta taagggaatc aaggcaggac ttgaagaagg tggttatacc acaacggata
     5161 atttagaaat tgattttatg aatgctgagg gtgatcaatc ccaagttcaa accatgagta
     5221 aaaaattggt ggacaatggc aatgaattat tgattggtat cgcaacacct gctgcacaag
     5281 gtcttgccaa tgcaaccaca gaattaccca ttatcatggg ggctgtaaca gatccagtcg
     5341 gagccaacct ggtgacggat ttgaaaaatc ctggtggaaa tattacaggg gtatctgacc
     5401 aaacaccagt agcagacgca gtttctctca ttaaagaaat tacaccagac gctaaaacta
     5461 tcggtgtcct ctattcttca aatgaggaca attcaaaaat tcaagtagcg gaatttaagg
     5521 cagcagcaga agaggctggc tatactgttc ttgaatatgc agttgcttct agtaatgaaa
     5581 ttgcagcgac agtagaggtc gcaagtagta aagcagatgt tctctttaca ccagtagaca
     5641 acacagttgc ctcagcattt tcaactgtcg tatctgtggc aaataaaacc aaaactccga
     5701 ttttcactag cgtagaagac atggtagaag gtggaggtat cgcatctgtt acccttagcc
     5761 aatatgactt gggtgtggca actggtaaga tggctgcgaa aattcttgat ggtgccaatc
     5821 cagcggatac acctgtacaa atctttaacg agggtaccgt tgttgtcaac caaaaagttg
     5881 ctaaagaatt aggaattacc ttgtcagatg atgtgattag taaagcaagc aaggttatcg
     5941 aataagagat aagagaaaag gaaaaatatg attgtatcaa cagtttcaca ggctctttta
     6001 tgggctgtac taggtttggg aatattcgtt acctttcgga ttctgaattt tcctgatatg
     6061 acgacagagg ggtctttccc gcttggagga gcggttgctg ttaccttatt gacgcaagga
     6121 gttcatccta tcttggctac tctagcagca gttcttgttg gctgtttggc gggctttgtc
     6181 acaggcttgc tttataccaa ggggaaaatt cccactcttc tagctggtat cttggtcatg
     6241 tcttcctgtc actctgtcat tctatttatc atgggtaggg caaatctagg cttactgaat
     6301 accaaacgtt tacaagattt ccttcctttt tctgagcaag tcaatggtct cttgctaggt
     6361 ctaggtagcc tagcggtggt gattggtgcc ttgatgttct tcctctatac tcgccttgga
     6421 caggccttta ttgcaacagg ggataatcca gatatggcac gcagttttgg tatcaataca
     6481 agtcgaatgg agttgctagg ccttgttctt tcaaatggga tcatcgctct tgcaggtgcc
     6541 ttgattgcac aacaagaagg ctatgcggat gtgtcgcgtg gtattggagt cattgtggtc
     6601 ggtatggcta gtctgattat cggtgaggta atttatggta accttactat gttagaacga
     6661 ttcttggcca ttgttgtcgg agcgattttc tatcagttct taattttggt ggtcatttcc
     6721 ctcgggatca ataccagcta cctccgtatt ttctcggcca tgattctggc aacttgcctc
     6781 atggttcctg agttgaaaaa taaacttctg aaaggagcca gtctgtcacg atgaaagcaa
     6841 ttgttgaatt aaaaaatgcc accaaggtgg tgacaaacgg ttttgatgaa gaacgtgtga
     6901 ttttggacaa tgtcaatctg accatctacc agggtgattt tattaccatt ttaggtggca
     6961 atggggctgg gaagtcaacc ctctttaatg tcattgctgg aaccctaccc ttgactagtg
     7021 gttcagtcca tattcttggt gaggacgtga ccaattggcc tgcagaaaaa cgtgctaagt
     7081 atttgtcacg tgttttccaa gatcctaaaa tgggtacagc gccacggatg acggtggcag
     7141 aaaatctact gatagctaag taccgtggcg agggacgggg cttactgcca cgcagattgg
     7201 cccaacagac ggaagaattt gcccagcttt tgccgagagt gggaaatggt ctagaaaaac
     7261 atctggacac gccagcagga cttttatcag gtggtcaacg tcaggcattg agtttgttga
     7321 tggcgagttt gaaacagcct gaacttttac tcttggatga gcatactgca gcccttgacc
     7381 ctaagaccag ccaatccttg atgcaattaa cggacgagtt gattgagacc aagggaatga
     7441 cggccctcat ggtcactcac cagatggaag atgctctgcg tcacggtaat cgcttgattg
     7501 tcatgaaaaa tggtcagatt attcaggacc aaacggctga agaaaaatcc aagatgaaac
     7561 tggaagatta ttataagttg tttgattaag aacctgtgtg gatagttcgc aacttccaac
     7621 taagtttggt tttggctaaa actgaatctt atatcaaaca gatcacttgt gaatacaggt
     7681 ctaaagtctg gagtaatcca ggcttttatt atcctttgaa tttttagtta aaaagcagta
     7741 taatatacta aatatatcaa gaggtgctgt catggataaa ttggtttttg aaagcttagc
     7801 tgacttagga atggactaca ccattgtaga gcatccacca gctctgacaa ctgaggaagc
     7861 agaccgttac attgaagggg tagaaggagt acggaccaag tccatgtttc tgactaataa
     7921 gaaaaagact gcattttatt tactgatcat ggatgaccag aagcagctgg atatggacca
     7981 atttcgtgat ttggtgggag ccaaccgcat tcggatggcc tcgagtgata gcttgatgga
     8041 aaaaatgcac ttgcctgcgg gtgcggtatc tgtttttggc ttgttacata atgttgataa
     8101 ggatattcaa gtcttttttg ataaagaaat cctatccgaa cccattctaa ccttccaccc
     8161 caatgttaat acgaaaacaa tttttgtcaa aacggaggat gtattacgtt ttgtcgaaga
     8221 gattggcttt tcagcaacga ttgttgactg ggggtaacaa agatgagaaa atgtttagct
     8281 atttgtagtc tgttgtgttg cctttttttg gtaggatgtc ggacaggagc agcttatcag
     8341 gcagagattg aaccgatgga acaatcagaa ttgtatacgg aaaaagagat tgaggcggct
     8401 attcttgtag ttcaagatta ctttgatcac cattttaaca gttgtaaatt gctgactatc
     8461 ggttacagtg gtgataatga aaaggaattt gatgagtggg cggagcacta tggtgcagag
     8521 gaagctatta tcctaacttc gtcttttaag gtagcagctg agggggcaga gcccacctta
     8581 gagcctaata gtactcatac ggattggaaa tggattttag tgcggaatgt tggtggtaag
     8641 tgggaacata agggacatgg ttactaatgt atatgtagaa cttcgttgat tttcagcgga
     8701 gttttttgta aattttcttg aaaaaatgta taatagaagc gatagctaaa atatagtctt
     8761 ttagtttact tttagtacta ggcaacgagc tgcaggcatg actcaatgtt ttcttgtttc
     8821 taggcattga cttgaaacag tcccccagac tgtttcaatc aggcaaggcg agttcaaaca
     8881 gtccagtgga gtgtttgaag ttagaaataa ggaaacggag tttcctctta cgtcgaagta
     8941 ataaaagaaa aactaaatga cgataaatag cagactgtaa ttcgacttgg agctcccaag
     9001 gaccgttggt aagaaaaata aaatggacac ctgtgggatg tccaaaagga gtaaagatga
     9061 gtacaattaa aattatggct cttgggggtg tgcgggaaaa tggaaaaaac ctgtacattg
     9121 ccgaagtgaa tgaacatatt tttgttttgg atgcaggggc aaagtatcct gagaatgaac
     9181 agttaggtgt ggatgtagtc gtcccaaatt tcgattattt ggaagaaaat aagcatcgtg
     9241 tagcaggggt ctttttatct cacgggcatg cagatgctat cggagcgctt ccttatctct
     9301 tggagaaagt gaaagtgcca gtatttggtt cgcacttgac tatcgaattg gctaagctac
     9361 ttgtcaaggg gaataatgct actaagaaat ttaatgattt ccatgttatc aatgctgaaa
     9421 cggaaattga tttcggggat tcagtggtat ctttctttca gacgacccac tctattccag
     9481 agagtttagg aattgtcgtt aagacggatg aaggaaatat tgtctacaca ggtgacttta
     9541 agtttgacca ggcggcagat aagttttatc gtacggattt tgggcgattg gctgaaatcg
     9601 gtaacgaagg tgtgttggcc ctcttgtcag actcagctaa tgcagatagc aatgttcaag
     9661 tagcgagcat gcatgaggct ggggaggaaa ttctcaatac gattgctgac tgtgacgggc
     9721 gtgttattgt ggcagcagtt gccagcaata ttgtacgtat tcaacagatt tttgacgcgg
     9781 cagaagcaac tggtcgtcgt gtcgtattga caggacatga tgtggaaaac attgtccgaa
     9841 cagcgattca attgaaaaaa cttcgtttgg tcagcgaacg cttgttggta aaaccaaagg
     9901 atatgaacaa gtatgaagac catgaattga ttattttaga aactggtcgt atgggcgagc
     9961 cgttaaatgg attgcgtaaa atgtctatcg gtagacatcg ctatgttgaa atcaaagaag
    10021 gtgatctagt ttatattgtt accactccaa gtatttcaaa agaagcggtt gttgctcgtg
    10081 tggagaactt gatttacaag gctggtggta ccgtaaaatc tatcacaaag aatcttcgcg
    10141 tctctgggca tgggaatgcg cgtgatttgc agctcatgct caacatcatg aagcctaaat
    10201 atcttttccc agttcaaggg gagtatcgtc aattggatgc tcacgctaag gctgcgcttg
    10261 aaatcggtat gtatccagaa aacattatca tcgttaagcg tggggatgtc atggcttttg
    10321 aagaggggga ttttgtccat agcggagcgg ttccggctgg tgatgtcatg attgacggaa
    10381 atgccatggg cgatgttgga aatatcgtcc ttcgtgaccg taagatcctc tcagaagacg
    10441 gtatctttat cgtggctatc actgtcaacc gccgtgagaa aaaaatcatt tctaaagcca
    10501 aggtcaatac tcgtggtttt gtctacgtga aaaagagcaa ggacatcttg cgtgaggcat
    10561 cagaattggt gaatacaacg gtggaaaatt actttacaaa ggatagcttt gactgggcag
    10621 agcttaagag tgctgttcgc gatgatttag caaaattcct ctttgaccaa accaaacgtc
    10681 gcccagctat tttgccggtg attatggaag tcaagtaagc tgatttatat acataagaag
    10741 agccgaggga aactcaggct ctttaccata aggagaagat ttatggcaat aatgaaaatt
    10801 gaataccact cagaagctct agatatgtct agacaggtta cggttttata tccagatcga
    10861 aatcgtgtgg aaaatcccga tgacaacgac attcctgtcc tatatctttt gcacggtatg
    10921 ggaggaaacc aggattcctg gctcaatcgg tctacaattg aacgcttggt tcgttatact
    10981 aatttgattg ttgttatggt taatacggat aaggcttggt acaccaatac cacctatggt
    11041 atgaattatt atgatgccat agcgattgag ttgcctcaaa tcctcaagcg gtttttccca
    11101 aatatgtctg acaagcggga gaagaatttc attgctggtt tgtctatggg gggatatggg
    11161 actttcaaga ttgccatgat gaccaatcgc ttttcacacg cagcttcgct ttcaggcgca
    11221 ttgtattttg attttgataa tccagcggca gcagaattgg gaagtctttc gtattggcaa
    11281 ggagtatttg gtgatatttc tgataaacag aatccaaata atttgttaga aattgctaaa
    11341 caatccgata aaaagaccaa attttatgcc tggtgtggtg aggaagattt tctctttgaa
    11401 gggcatcaaa aggcagttga ggagttgaaa gagctgggat ttgatataga ggcaagtttc
    11461 ggacctggta aacatgagtg gtactactgg aatcagcaga ttgagaaagt attggcttgg
    11521 ttgccaattg atttcaaatt ggaagaaaga ttgtcctagt ttttaagaaa agtttcagaa
    11581 attatggcta tactaaaacc tatttctaga aagaggtata gtctatgaaa cttattggaa
    11641 atattctacg gtggatttgg cggacatttt ggggtctgat ttggttatca atggtgttat
    11701 tggcttgtgg tctaggtcta ttattctatt ttcaacagga agcagcgcct cagatgttac
    11761 aaactctacc tcaagaagtg caactgatgg ttaaaggaca gtctgaagtg acgactgata
    11821 cggtcaatac agccgatcat ggtcgatggg aaagcaatac tgcgactgtt tacattgaaa
    11881 ctcagaatcc aacatttgta gctgcctatg aaacagccat tgcaaattgg aatgcgacag
    11941 gggcatttac ttttgtgatg acaagtgatc caaatcaggc tgatattatt gctactgaga
    12001 tgaatgatgg aaatacccag gcagcaggtg aggccaattc aacgactaat ttattgacga
    12061 attattatag ctcggtaacg gtgtgtctga atagttttta tttattaaac gaccaatatg
    12121 gttatgatat ggatagaatt atccatacag ctgagcatga attgggccat gctattggcc
    12181 tagatcatga agatagtcag acctctgtta tggaatcggc aggttctaat catggtatcc
    12241 agcaggcaga tattgatgcg gtccttgcct tgtattcaga atagaaggga aaatatgctt
    12301 cgaacaatta caaacacaat aaaacgttat cctgagcaag ctctgctctt tctctataat
    12361 gctggaattt ttgcatggat gcagtctacc agtcattcga ttatggaaca aatcggtata
    12421 gatagcaact ggtttgataa aatccctgaa ccaattaagg cctggacagg tgcaagtcta
    12481 gagagtatgc aaaccttact caattcttct gcttggggat ggctgattgt atctatgata
    12541 ttgatgttgg tcatccgctt tgttaagggc ttgattaagt ttgtcattat gctgattatt
    12601 atcggcggtg gtctttatct actctggcag aataaggaat tgcttagcgg tttggtctaa
    12661 atattttttg agaaggacag tgacggtaaa gtcgctgttt tttgctgaaa tcagaaaaag
    12721 gattccgcac tgttacccta aattatggta caatattttt atgattattt taagtggaaa
    12781 caagattgaa cgctcatttg cgggcgaagt tttatttaac aatatcaaca ttcaagtgga
    12841 tgagcgggat cggattgctc ttgtcggaaa aaatggggca ggtaagtcca ccttgcttaa
    12901 aatccttgtc ggagaagaag cagcgactag cggcgagatt tctaccaagc gagatttgtc
    12961 cctttcttac ctggcacagg acagctgttt tgagtcggaa aataccattt acgatgaaat
    13021 gctccatgtc tttgacgatc tgcggatgac ggagaaacgc ctgcgatcta tggaagagca
    13081 aatgggcagt ctttctggta atgaactcga ccagctcatg aagacctatg atagtctgtc
    13141 ggaggaattt cgtctggcag gtggttttag ctatgaggca gacatccgtg ctattttgaa
    13201 tggtttcaag tttgaccaga ccatgtggga catgaagatc tccgaactct caggtggtca
    13261 gaatactcga cttgctctgg caaaaatgct tcttgaaagc ccagagttat tggtcctgga
    13321 cgagccgacc aaccacctag acatcgacac gattgcttgg ctggaaaact acctagtgca
    13381 ttacaaggga gctttgatta ttgtcagcca tgaccgctat ttcttggaca aggtggcgac
    13441 actgacactg gatttgacca agcattcctt ggatcgctat gtgggcaatt attctcagtt
    13501 tgttgagctc aaagagcaga agttacaaac agaattgcaa aactatgaaa agcagcagaa
    13561 ggaaattgcc aaactagaag actttgtcca gaaaaatatt gtccgtgctt cgaccaccaa
    13621 gcgcgctcag gctagacgca agcagttgga aaagatggat cgattggaca agccgacaac
    13681 tggtcagaag tctgccaata tgaccttcca gtcggacaag acttctggca atatcgtctt
    13741 gacagtggaa aatgctgcgg tgggttacga tggaaacatt ctttcccagc ccatttctat
    13801 tgaccaacgg aaactcgatg ccattgccat tgtcggtccg aatggaatcg gaaaaacaac
    13861 cctgctcaaa tccatcgtcg gagctctgcc atttatcaaa ggggaagcct ggctcggtgc
    13921 caatgtggaa gtgggctact atgaccagac ccagtcggcc ctgacacctt ccaatacggt
    13981 cttagaggag ctctggtccg cctttccgac tacacctgaa gtggaaatcc gcaatcgcct
    14041 tggagctttc cttttctcag gagatgatgt caaaaagtcc gtttccatgc tatcgggtgg
    14101 ggaaaaagcc cgactgctcc tcgccaagct gtccatggaa aacaacaact tcctcatcct
    14161 tgatgagccg accaaccact tggatatcga cagcaaggag gtcttggaaa atgccctcat
    14221 cgactttgac ggcaccctgc tttttgtcag ccacgaccgc tatttcatca accgcgtcgc
    14281 caccaaggtg ctggaaatct cggaaactgg ctccacgctc tatttgggcg actatgatta
    14341 ttatttggag aaaaaagcag agctggaagc agaagcccag cctgaccagc tagaaagtac
    14401 cagccaatca gctggagcca tggactacca agcccaaaag gaaaaccaaa aagagcaacg
    14461 caaactagcc cgccgcatcg agcaaatcga ggcagaaatc gatagcatcg aaaaccgcct
    14521 gaacgagctc aaccaagcca tgctagaaac caacgacatc ggccagttga ccgattacca
    14581 gaaagaaatc gaccaactga ccagccaaca agaaagtctc atggaagaat gggaagaatt
    14641 atctgagcag atgggctagg ttgacagcaa gttgacaaga ggctcaaacc cttgatagaa
    14701 caagcttgca agccagtctt gcggagttag aacgaccact tttcgctaaa aaagtccaga
    14761 gcaagccagt ctttcttgga aaacatcgac cgacaagcta agatcagtat agagattagc
    14821 atcacttctc gaaggtggtg tcaacatctc agcgcagtgg ttgattggca gatttgttcg
    14881 tgttgcacac ttcaaatctg gcctaacgaa tgttgcgaac tttgttcgct ttattcccaa
    14941 ccttaaactg tcccccagac agtttaagct gtgcgggggt gggagtgaaa cagtctgggg
    15001 atagactgtt tcagctcaac aacttaaaac gaaaaattgt tgacgaactt tttttcattt
    15061 ttcgagttct gtcccgctcc cagaaaggag aaagaaatga actttggaca gcaaatcaaa
    15121 gacttacgaa aaaagaaagg tttgactcag gaacagtttg ccctcaaact caatgtgacc
    15181 aggcaggctg tttccaactg ggaaaatgac aagaatctac ctgatttgga gctcttgatt
    15241 ctcatgtcct ctgtcttttc gatctcctta gatcaactta tcttaggagg aactgatatg
    15301 aacaacatga cagaaaaact tgtaaaagac ggtcgtgaag gccgccgtac ccagatgcac
    15361 ctgaccatta ccattattgg tagttttctc atgcttctcg gcttcgtctg ctttgtcatc
    15421 aaggcaaact cggtcgagta tattgatgcc gagggcattc tgcatgaaaa tttttatctc
    15481 attccagtcg gctacttgct ggtctttaca ggagcccttg ccacgctcct atcaggacta
    15541 gccctgcacc gctttagaaa agaacacaaa taagatgacc aaacaccaac gttttgccaa
    15601 tagcgaccaa tttttcgcct gtccacattg tgggcaggct cttggtcttg acctcaacag
    15661 ccttcgctgc cccaaccgcc acaccttcga cattgccaag cagggctacg tcaacctggc
    15721 accccaggtc aagcagtccg ccaactacca caagtctagc tttgaaaacc gtcaggcctt
    15781 tttagaggcg ggctattacg atcatctcta cgaggctttg gaggaaaaaa tagcagagct
    15841 gggattgcgg tctgtcttgg acatcggctg tggagagggc ttttattccc gtaaattagc
    15901 cgagaaaatg gacttggaca ttcttgcttt tgatatttct aaggattcta ttctcctagc
    15961 agccagaaca gacaggacca agtcggtcaa atggtttgtc ggtgacttga ccaagttgcc
    16021 cattcaagac aagaccatcg acgggatctt ggacatcttc tccccagcca attatcagga
    16081 atttgccaga gtgctgaaag ctggtggggc tattctcaaa ctagtcccag gccccaatca
    16141 cctcaaggag ctccgccatt tagccaaaga ccaactccgc aaggagtcct atgacaacca
    16201 agatatcgtg gaccatttca agtcttacat gggacaggtg gagcaagagg tggtcagccg
    16261 gaccctgccg attggtatag agcaggctcg tattttggca gacatgacac ccctcttttt
    16321 ccaagtggac cagtccaagc tggatttgag tcaattgacg gagataacgg tggagggctt
    16381 acttttgatt ggaagtatat aaaataaaac cgcctctgga tatcagaagc ggttttgctt
    16441 tatttctcat actcttcgaa aatcaaagtc agacgttgtt gacttgattt gatgaacttc
    16501 agttctatct gcatctgcgt cgcctagtct gattctgatt ttcattgagt atcaaattct
    16561 gccttgcttt ttacatcgcc gtattcttca gcgacttctt tgctgaggat gtcggcgtag
    16621 ctggcaagtt ggatgtcttg tccgtatttt ttgcggaggg cagtagtgac caggcggtag
    16681 gccaggttgg cattgcggga taagattggt ccgtggaagt agctaccgaa ggtgtttttg
    16741 tagtgcagac cttcgccgcc gtcttccttg ttattgccat tgccgtagac aaccttgccc
    16801 agcggttttt ggttgtcagc taggaaagta cgcccttggt ggttttcaaa accgtagtag
    16861 gtttcgttga actcatcatt gtgaattttg atgtcaccga tgtagcggtt attggtctgg
    16921 ttgagggtgt agtggcccat gattcctaga ccgtcaatct tgcgaccgcc agcttcaata
    16981 taatattgac cgagcaactg gaagccaccg cagatagcta gcatgacgcc atcgttttcg
    17041 ataaagtctg ctaggctgtc tttttttgtc ggtaaatcct tagcaagaat ggattgttca
    17101 tagtcctgtc caccgccgaa aaaggcgatg tcgtagaaat ttttgtcaaa ttcatctgtc
    17161 agggaaacga tgtccacctg gacacgagcc cccagctttt cagccacata cttaagcatg
    17221 aggatattgc cattgtcacc gtaggtgttc atcaaatcac cgtagaggtg ggcgatgtgg
    17281 agatcgtaag tgtagtcttt ttcaggactt tttaatgacg tatagaccat tatttcatct
    17341 cctttccaac ggcttggcgt tgagccagca ggtcacggaa ttcgagcata gctgtataag
    17401 tagctaaaat ataagcatgc tgactactcg attgctcaat caaggtcatg atgtcttcta
    17461 gttttggtgt ttcagtaatc tggttctcag gatagccagt cacacggagg cgacgggcaa
    17521 tttcagacga acgaacaccg cctgcaaaga cttgtggaat gtccatttgg gtgatttgtt
    17581 caaagtcagc atcccaaatc cagctggtat caattccgtc agcgtagttg gcattgagaa
    17641 ggacggacag gctaaaatcg tatggagcca gtttcatcat ttcaatagcc tgggttgcac
    17701 cgacagggtt tttaatcaag accaatgtac aatctttatc acccaactta aatgtttctt
    17761 ggcgaccgaa gactgccttt gatttgtcaa atcctgcctt gattttttca ggagatacgc
    17821 cgaaaaattc tgctacgctg acagctgcta gggcattgta gatgttgtag aggcctccga
    17881 tgttaatctt ataatcctga ccgtcaatga caaaggtaga agaagtattg gtgatttctt
    17941 ttagtttggt caacttgtag tccagttcag gtcggctaaa gccacagttg gtacagatgt
    18001 agctacccaa gttggcgtag gtattgagct tgtactggat aatctggtgg caatgaggac
    18061 agagaacgcc ttcggtattg taatgggcca gacgtggctc atgctcttcc gtcgcaaaac
    18121 cgtagtattt tactggattg ataacgttcg ttgagttgaa aagtggacta tcaccattgg
    18181 ccaaaatagt tgcctgcgga gcttttgcag ccccgtccaa aatcatctgg taggtggtat
    18241 aaatctcacc gtagcggtcc atctggtcac ggaagatatt ggtaaagaca atcagactag
    18301 gctttataaa gtcggttaca cgggcaagac ttgcttcgtc aatctccaag acggcaatct
    18361 tcttgccgtt tttatttttc ttggcagaca aaaaggttgc cgtaattcct gtgatcatat
    18421 tggcaccgct ggtattggtc gtgatttcgc caaaggcttc ttggaggata ccaactgtca
    18481 gggcggtggt cagagtcttt ccgttagtac ctgtcacaac aacgacttca taatccttag
    18541 ccaaactatc taaaatatgt ttatcgaatt tgagggcaat ttttccaggt agggtagtac
    18601 cacgtcctag ttttttcaaa atgaactggg aactttttcc tgctagaata cccaaagctg
    18661 tatttatttt catgggacta ttttatcata aaggttgggg attttctaga atggagtatg
    18721 ttggaacatg atatattcct gcagtaaatc tgtaatattt ttaacaggaa ggtcactatt
    18781 ttctaaagaa aaggggaaaa aatatggtat aatgagtaca ttactatgta tgagttagaa
    18841 agtatgagaa tgatatgtta aacctaaacc agttactgga tacagggtat tggtcgagtt
    18901 tgatagccag tccttggacc gcactgttgc acctaattga tattagtata gttgtttatt
    18961 tgatttataa ttttagtaag tctattgcag gtactaaaat tatgacattg attcgagggg
    19021 tttttctctt cattattgtt cagattatag ctagtttatt cggtctgcag acgattgctt
    19081 ggctgattaa tcaagtaatc acgtatgggg ttattgcagc agtagttatt tttgcaccag
    19141 agttgcgcgc tatgcttgaa aaactcggta gaacaactca gatttttggg acaaatacag
    19201 tgagtgcaga agaaaagtta attgttgctt ttctcaaatc ggttgcttat atgtctccgc
    19261 gtaaaattgg tgcacttgtg gcagtagaac aagcccaaac attgcaagaa tatagagcaa
    19321 cggggattcc actcaatgcg gatatatctc aggaattgtt aataaatatt ttcatcccga
    19381 atacaccgct acatgatggg gctgtaattg tcaaggaaga caaggtagct gttgcttgtg
    19441 cttatttgcc cctgtcagag agcgcaggaa tttcaaagga atttggaaca cgacatcgtg
    19501 cagccattgg cttatcggag gtatctgatg cgtttgtatt tattgtttct gaagaaactg
    19561 gcagtatctc tattgcacga aacgggatat ttaaacatga tttgactttg gatgagtttg
    19621 aagcagagtt gcgagcgact tttatttctg aaaatgtaga gaagaagtcc atctggaagc
    19681 gattaggagg tggcaaacat gaatgataag tttaaagctg taggtcactt agccttatct
    19741 atttttctag cattgttgct ctttttctat gcaacaacca ccaactataa aaattcagaa
    19801 tcagctgcac agaacacgga gtcagaaacc tatgttcaca cactgagcaa tgtaccgatt
    19861 gagattgaat atgatacaaa caagtatttt atttccggat tttcttcgac ggtggcagtt
    19921 gaattaaggg gctctaatcg tgttctccta cagagagaat cagatgagtc aactcggact
    19981 tttcaagtga ctgcggattt gagagagttg agtgatggga cacagactgt caagctacaa
    20041 ttgaccaatt tgccggctgg agtgagtgcg acactagcac ctgatgcgat taccgtcaag
    20101 attggtaaaa aagttagcaa ggcattccca gtagcaggtc gagtctatag taatcaattg
    20161 gcagaaggtt acagtctgtc taaggtttca gtcaacgtag ataccgttaa agtgacaacg
    20221 gatgaagaaa caatggctaa gattgatcgg gtcgaagctg ttgcgatgga tgtgagcaat
    20281 ctgtctgaga attatagtgg tacagctaaa ttacaggctg tagatagcga cggcaatgtc
    20341 ttgccagttg tactgtctca gacggaagca aatatgcaaa ttatacttac aaaaacaaaa
    20401 taagaggtta tgaaaatggg taaatatttt ggtacggacg gagtccgtgg agaagcaaac
    20461 gtagaattaa cgccagaatt agcattcaaa ttgggtcgtt ttggaggtta tgtccttagc
    20521 cagcatgaga cagatgttcc tcgtgtcttt gtggcgcgtg acacacgtat ctcaggtcaa
    20581 atgttagagg ctgccttgat tgcaggactt ctatcagtag ggattcatgt gtataaattg
    20641 ggagttttgg cgacaccggg tgttgcccac ctagtcaaaa ctgaaaaagc aagtgcaggt
    20701 gtcatgattt ctgcaagtca taacccagcg caagataacg gtatcaaatt ctttgctggt
    20761 gatggtttca aattggatga tgcattggaa gcggaaattg aggctcttct tgacgctgag
    20821 gaagacacat tgccacgccc atctgctcaa ggtttaggag atgtggtaga atatccagaa
    20881 ggtctacgta agtaccagca attcttggta tcaactggta cagatttgga cggtatgaaa
    20941 gttgcacttg atacagctaa tggtgctgct gcaacatctg cacgtcaaat ttttgtagat
    21001 ttgggagcag atttgacagt aatggctgaa aagccagacg gtttgaatat caatgagggt
    21061 gttggttcaa ctcatcctga aaaattgcaa gaactggtca aagaaacagg tagtcaaatt
    21121 ggacttgcct ttgatggtga cagtgaccgc ttgattgccg tcgatgaaaa tggtgttttg
    21181 gtagatggcg accgcatcat gtacatcgtt ggtaaatacc ttgctgatcg aggtctattg
    21241 gccaaaaata caattgtgac cacagttatg tctaaccttg gtttccataa ggctttggac
    21301 cgtgaaggga ttgaaaaagc tgtgaccgca gttggtgacc gttatgtggt tgaagaaatg
    21361 cgcaaggaag gctacaatgt cggtggtgag cagtcaggac atgtgattct gatggattat
    21421 aacaccacag gcgacggcca gttgaccgct gtgcaattga ccaaaatcat gaaagaaact
    21481 ggtaagaagt tgtcagaatt ggcagcagaa gtgaccattt acccacaaaa attggtcaat
    21541 attcgtgtgg aaaatagcat gaaggacaag gcaatggaag tgcctgctat tgcagctatc
    21601 attgaaaaaa tggaagcaga aatggctggc aacggtcgta tcttggttcg cccaagtggt
    21661 actgagcccc tcttgcgtgt catggcagaa gcgccaacgg atgctgaagt cgattactac
    21721 gtggacacca ttgccgatgt ggttcgtgcg gaaattggga gttagacaga aatcagtaac
    21781 tcgtagagtt tgattttgtt gtctaacccc cgcaaggttg agtaggaatt tcacagctga
    21841 taggcgaaga agttccgctc aatactgcga gaataaaagt aaaaactgga gtttggttac
    21901 tccagttctt tgatgttgtg aggaaattat gagtatttta gaagtaaaga atttaagtca
    21961 tgggtttggt gaccgcgcaa tttttgaaaa tgtctccttc cgtctcttga aaggggaaca
    22021 tatcggactt gtcggtgcaa atggtgaagg gaaatcgacc ttcatgtcca tcgtgacagg
    22081 tcaattgcag ccagatgaag gtaaggttga atggtcacgt tatgtaacgg caggttattt
    22141 agaccagcat gctaaactag aaaagggtca atcagtccgt gatgtcttgc ggacagcatt
    22201 cgatgaactc ttcaagacag aagctcgtat caatgacatt tacatgtcta tggcagaaga
    22261 gggtgccgat atggatgccc tcatggaaga ggttggtgag ctgcaagacc gtctggaaag
    22321 ccgtgatttc tatacgcttg atgccaagat tgacgaagtg gcgcgtgcgc ttggtgtcat
    22381 ggattatggc atggacaagg atgttacgga gttatcaggg ggacaacgga ctaaagttct
    22441 tcttgcaaaa ttgctcttgg aaaagcctga catcttgctt ctggatgagc cgaccaacta
    22501 cttggatgca gagcatattg actggctcaa acgctacttg cagaactatg aaaatgcctt
    22561 tgtcttgatt tcgcatgaca ttcctttcct gaatgatgtg ataaacattg tctaccatgt
    22621 ggaaaatcaa ctcttaaccc gttacacagg ggattactac caattccaag aagtacatgc
    22681 tatgaagcgg gctcagttgg aggcggccta tgagcgccag cagaaggaaa tcgcagactt
    22741 gcaggacttc gttaaccgta ataaggcccg agttgcgaca cgaaacatgg ccatgtcccg
    22801 tcagaagaaa ttggacaaga tggagattat tgaacttcaa gcagagaaac caaagccatc
    22861 atttgatttc aaaatggctc ggacgccaag ccgctttatc ttccagacga ctgacttgga
    22921 gattggttac gaccgtgtcc tgacccgtaa gccgctcaat ctgacctttg aacgcaacca
    22981 gaagattgcc attgtcggtg ccaacggtat cggtaaatct actcttctta aaagtttgct
    23041 ggggattatt ccaccgctcg gtggctctgt ggagcgtggg gagtatctgg aactcggtta
    23101 ttttgaacaa gaagtagctg gcggcaatcg tcagacccca ctggaagtcg tttgggatgc
    23161 ctttccagcc ctcaatcaag cagaagtgcg ggcagccttg gctcgttgtg gtctgacttc
    23221 caagcatatc gaaagccaaa ttcaagttct ttcaggtggt gagcaggcca aagttcgctt
    23281 ctgtttgctt atgaaccgtg aaaacaatgt cctggttctc gacgagccga ctaaccactt
    23341 ggatgtggat gccaaggatg aactcaaacg tgcccttcaa gcctataagg gctcaattct
    23401 catggtctgc cacgagcctg atttctatga aggctgggtg gacgatgtct gggattttaa
    23461 cgacttgacg tagactaaaa gctatcgata ggtagctttt tctctttctt tatgccataa
    23521 tagaattagc atagtaaagg aggtgaaaaa tgaaaagaac agaacgaggt catattggct
    23581 aggcctcaag cgaggaaaga aaaatgacag attatgtaaa ctacaatcaa gaacgttgga
    23641 atcgggtttc agctagacag ggaaatgcct atacagttcc gctcagtcat gaggaatttc
    23701 ttgttgccaa agataagccc ttatccgttt ctttgacggt tggaaagaca gtaccgcttg
    23761 actggtttga aaaagccaaa ggtaagaaat tgctgggttt ggcctgtggt ggtggtcaac
    23821 agggaccaat ttctgcggct catagctatg agacaactat tttggacttc tccaaggaac
    23881 agttggacaa ggataggcta gttgctgagc gagaaaatct ggatttgcaa acggtccagg
    23941 cagatatgac acaagccttt ccttttgaag acgagagttt tgacatcatc ttttgtccgg
    24001 tttcaaatat ttatatcgaa gatttggcca atatgtggca ggagtcttat cgggttttga
    24061 aaaaaggtgg tctgctcatg gtcggttata tgaacccttg gatttatgtc tttgatgctg
    24121 atgatgtttg ggagcatcca gacaaaacct tggttccaaa gtacagcctg cctttcaacg
    24181 cacgacaatt agaagaagca ggacaaatta cgattgatcc agaatatggc tatgaattta
    24241 gccacacctt agaagaacag attgctgggc aattacgagc tggttttgcc atgattgatt
    24301 tttatgaatc caaagacagt cgcaatcgct tgactcaatt tgcctcagat tacctagcga
    24361 atttatcgat aaagtggtag gcaaatactt ctgacagaaa gtattttaac agggctagtt
    24421 agataagcac tggtcctatt tttgagctat tgtcaatgga tgagaagagc ctaaattgtt
    24481 gaacaaaaaa cacttggaaa cgcttgcact ttataattag gattgatata atgaaagaaa
    24541 aaacaagagg ttttcaaatg aaaacgaaat tactgcttgg cgtagccctt cttagtgtcg
    24601 gtctatcggt agctcctcct acaatagtag aggttgaagc acagacgact cacgtttcag
    24661 atccagctct acgcaatagt tatccatata atttatctca ttatttggaa gtaaatgttg
    24721 gttccggtca aaccaaaaaa gttcagctat tctaatagct ccgaatactt tattaaccgc
    24781 agttcacgta gtggcagatg atgggactgg acgtgtgacc agccaaactt ggtctggaaa
    24841 tgctgtttgg ggcgatgctt caccatacta tgaactgtca gaaattaaga gaaccaatcc
    24901 cttcaatccc ttcattcaga tgccatgata tggtggaaca tgggatgcta gtcgagacgt
    24961 agcccttgtg aaaatcacca cccctagcag aaaaacacag acagccaata cggcaaatgc
    25021 tcggctagga atttaccgta acacctatga cctagttggc cgacaatttc taatggtcag
    25081 caacagcatt aatctctacg gacgttggga atacgaatat ggaaccatta cggaagtccg
    25141 ttatgacggt ttgcttcaaa ccaatatcac aggtgttaag gggcaatccg ggtcacctgt
    25201 cgttgtagac ggtcgcatta tcggtgtcgt atccaatatt gattctaatt caaaaatcgt
    25261 tattactccg ttgactacgg agatgaaaac acaattattt gataagaatg gtatcaaaaa
    25321 tattgatatt tactagcgat attcttgagc catcaaagtc ctgctttact gataaggcag
    25381 ggcttttcat tgcaatccta acacattttt cgcacataaa gaaaaattac aggtcagtgt
    25441 tacctagcta tcatttgaaa taaaagtaag tagtggagtt agctgtttag gtaacaaaat
    25501 gattgcgtaa atcgttgagg tctgggcagt aagtagataa agcttataaa gtattttatt
    25561 ttgattacag atgcatattt attcgtaaaa aaatgggcgc cacacataga ttgcatcatg
    25621 cagaaaatag attttagaag ccaaggctat tgacctgact ggatttttct gctatgattg
    25681 aaaaatgttt gtttggaggt aaatgagtga ataaaataat atctgtatgg aaaaaaatga
    25741 atttaatccg aaaaatcgga attggtgttg ttttgggagt gttactaggc ttaattgctc
    25801 ccaaaataac ggtcattgct ctctttggta gtctatttgt aggagcattg aaagctatag
    25861 cccctctttt ggttctgaca ctagttgcac atgctctatc acaagctcct gctggtcaaa
    25921 aatcgaatat gaggacagtt atttgcttgt atctatttgg cacttttgcg gcggccttca
    25981 ttgctgtagg agcaagctat ttattcccta ttaaactggt tctttctact acaacgacta
    26041 cggacattac tcctccacaa gggattgctg aagtattcca agacctcttg ttgaaggtcg
    26101 ttgacaatcc aatcaatgct cttgcaacgg ctaattatat tggtgttttg acatgggcgg
    26161 cagtgtttgg cttggccttt cgtcatgcaa gcaagacaac aaaagattta ttgcaatcta
    26221 cggcggaagt gatttctaaa gttgtaggat ggattattgg tctggcacca tttggtatca
    26281 tgggcttggt tttcgatacc attgcaaata atggtctgac agctttaaaa gactatggtt
    26341 tactgctctt actcttggtt ggaagcatga tttttgtcgc gcttgttgtc aatcccttga
    26401 ttgcttttct tgtaatgaag aaaaatccgt acccgctggt atttgaatgt cttcgagtta
    26461 gtggggttac ggcatttttt acaagaagtt cagcagctaa tatccctgtc aatatgcagc
    26521 tctgcaaacg tttaggagtg gatccagata catactcagt atccattccg ctcggtgcca
    26581 caattaatat ggctggcgcg gctattacga ttaatatttt aacaatggca gctgttcata
    26641 cacttggcat tagtgttgat ttcagctcag ctcttctttt atccgttgtt gcatcactat
    26701 cagcagcagg agcatctggg gtagcaggtg gttcgcttct cctcatccca gtagcatgta
    26761 gtctttttgg tattccaaat gagttagcca tgcaagtagt tggagtcgga tttgtggtcg
    26821 gagtgattca ggactcttgt gaaacagccc tcaactcatc aactgatgtt ttgtttacag
    26881 ttgtagcaga acgatctgct tggaaaaaat agaaagaagt tgggagacca actttttttc
    26941 ttcgctttac ttgtgaaatc tctttcattt tgtgataaaa tagacttact ttgatactga
    27001 aaaggagaac atatgacagt aaattttaga gcagagtttg acaaacgcaa agatgagttt
    27061 ctagcggacc tctttgacct cctccgcatc aattctgagc gtgacgatag ccaggcagat
    27121 gcccaacacc catttggacc tggaccagtg cgtgccttgg ataagtttct agaaattgct
    27181 cagcgtgatg gttatccaac caagaacgtt gacaactacg caggtcactt tgaatttggc
    27241 gagggtgacg aagtcctcgg tatcttcggt cacttggacg tggtgccagc aggaagcggt
    27301 tggaacactg atccatacga gccgcaaatc attgacggca agctctttgc ccgcggatct
    27361 tctgacgaca agggaccgac catggcttgc tactacggct tgaaaatcat caaggagttg
    27421 ggcctttcga cctctaaaaa agtccgcttc atcgtcggta ccgacgaaga gtcaggctgg
    27481 gcggacatgg attactactt cgagcacgtc ggccttcccc tgccagattt cggattctct
    27541 ccagacgctg agttcccgat tatcaacgga gaaaagggca atatcacagc ttatcttcat
    27601 tttgcggggg aaaatagcgg agctgctaaa ctgcattcct tcacaggcgg cctgcgtgag
    27661 aacatggtgc ctgaatctgc gacagctatt atctctggcg acctagcaga tctggacagc
    27721 aagttagtag atttcacagc agcctatggc ataaaagctg atgcggaaac ccttgaaaac
    27781 ggtcaagttc aagtcaccgt catcggaaaa tcagcccacg gttcaacccc agaagaaggg
    27841 gtcaacggag caacctactt ggcaaaattc ctcagccaat tcgcctttga cgaagcagcc
    27901 aaagcctatt tggatttggc aggtcaagtc cttctggaag accatgacgc taaaaaactc
    27961 ggcgtagcca tctacgatga gcaaatggga gctctttcta tgaacgctgg tgttttcaaa
    28021 tttgacgaaa cttcatcaga caataccatt gccctcaact tccgttatcc aaaaaatacc
    28081 aaccctgaaa ccatcaaggc tggtttggaa aaactgggcg tggaagctgt tagcctgtct
    28141 gagcatggcc atactccaca ttattgccca atcgatgacc caatggtcgc aaccctcttg
    28201 tctgtttatg aaaaacacac aggcttgaaa ggtcacgaac aagttatcgg aggtggtaca
    28261 ttcggacgct tgctcaaacg cggggttgcc tacggagcta tgttcccagg cgatgtcaac
    28321 accatgcacc aagccaacga atttatcgaa gttgagcaac tctaccgcgc cgctgccatt
    28381 tatgcagaag ctatctacga actcattaag taatacatga agacgggtac gcccgtcttt
    28441 tttaggagga aatatggaac tgaaagaact ttatttttca gacatggaaa tggttaaaga
    28501 attattttta tctgttttca gccaagaacc ttggaatgat gattggtcag atgaggaaca
    28561 attagattgc tacataggcg atttactggg acactcaaga gcactctgtt ttggcttgtt
    28621 tgaccaagaa aaattgatag ggctgtcact tggttacatt cgctattggt atgagggaac
    28681 agagtatcga attgaagagt tctgtatttc ccgtgattac caagggcaag gacatggtgc
    28741 tagtttatta gctgatattg agaagcagtt agctgataga aagattgttc atattcttct
    28801 gcaaaccgaa cgcaaccttt tagcctattc tttctacaaa aaatgcgttt tccgagcctt
    28861 agaagaagat gtgaccatag tcaagaaagt aggacatcat ggaacttgct gatattcgtc
    28921 aagccatcat tgaggatgtt gctaaccgag attttacgaa caagggaata gctccccttt
    28981 tccaagcccc ttcgaccgca aaaattctca ttattggaca ggcacctggc ttgaaaaccc
    29041 aggaaaaagg gcgtttattt gacgacgcca gtggtagcaa ccttcgtcaa tggttgggcg
    29101 tggatcgaca gactttttat gaatcgggtc attttgccat tcttcctatg gatttctatt
    29161 atccaggaaa agggaagtct ggggatttgc cgcctcgcaa ggattttgcg cccaaatggc
    29221 acccactcat tatgaagcaa ctcccccaga ttgagctgac catattggta ggcaattatg
    29281 ctcaaactta ttatttggaa aaatcccaaa ggaacctgac ggagagagtg aaacatgcag
    29341 atgattacct acccacctat tttcctttgc cccatccatc acctagaaac aatatctggc
    29401 aagctaaaca tccttggttt gttgaacaga ctctaccgat actgaaaaac aacataaata
    29461 tattgttggg aaaatgataa tatgatataa ttgtatatat atacctaata aatttttctt
    29521 atctttgttc atcgaaggag gtgttctgat gagaaaatgg aaaaaaatta tcatcggctg
    29581 tttcgctatt ctaacggttt gcttgctatt tgcacccaca ctagctagag caaatcagat
    29641 cccagaccgc cccacaggta caacggtagt ggatgagacg cagttactct cgagtgagac
    29701 cattgcagaa attgatcaac tcaatcgaag ttgggcaagc accgagcaag ctttacaggt
    29761 tggtgtttat gtaacagaaa gtctgtccag tgatattgaa agtttggcta atgaaacctt
    29821 tcgtcactgg caggtcggtt ttgcaagaac ggacaacgga gttcttttag tggttgccat
    29881 agctgacaga gcatttcgga tagaaacctc agacaatgct gcaacggtgt tgacagatgt
    29941 ggaagccaag gatattttag agaatgcaag ggaattcttt cgacaggaag actacagtag
    30001 aggaatttcc tatatcgtca actccattgg ggatagattt tatggtacaa gcattggtaa
    30061 aaatcagtta gctgctatag aagaaggtac aagtgagaag gatgacggct tctggctgtt
    30121 tctcatagtt attctgatcg ctatcctatt tggtatcatt gacaaatcta gtcgtggtgg
    30181 tggaggtcca ggcaatctac tctggatgtt ggttgatgat catcatcact atcacaatca
    30241 tcactcgtct aattcctcat cgtctagttt tggcggaggt agttggtcag gaggcggcgg
    30301 tggcggagcc tcttctggat ggtaaggcat cgtataaggc ttctgatagt tgtttgcttt
    30361 tttacgaatg tttaatcgta gttagaaagg aaatcttatg aaaaagagat ggttggttat
    30421 tctcattcca gtcctagctc tgctgttttt aggaatggga gcagttgggc aatacaatgg
    30481 cttagttgac agttatgctg aggttgaaaa tgcgcaagcc aatgtagaca cacagttgca
    30541 acgtcgctat gacttgatac ccaatgtagt cgcagctgtc aaaggcgcca tggaacacga
    30601 ggaagaaata tttactgcta ttgcagaagc gcgtgctaaa atcggctcca gtcaagtagg
    30661 aaccagcgac tacaatcagg cacaaagtca gctggattcg gcagtgtcta gattgttggt
    30721 agtggctgag aattatcctc aattgacagc caaccaacag gtatctgacc tgataacaga
    30781 gcttgaaggt acggaaaatc ggatactggt agcccgtaaa gactataatg cggtagcaac
    30841 tacctacaat aaaaaaatca agcggttccc gacctccatc tatgcaggtc tattcggata
    30901 tgaaaaagta gagctcttcc aagcaacaaa tgatgcggcg acaaccgtgc caagtgtgga
    30961 tttgagtgat aaggaataaa ggagataaat atggactttg gatttgatat attggcaatt
    31021 atgataccaa ttattgttgt tgtactattt gttttattgt ttgtatttgg ttatgtgtct
    31081 gctaagccta acgaagcgat tgtcattacc ggcttgggca aacctcggac cttaattggt
    31141 cgttctggtt ttatgattcc ctttatcgaa aaacgctcct atatctctat tgagcagttt
    31201 tcaacagatg tgcagaccac agattttgtg ccgactcttg attttatcaa cgtaaaagct
    31261 gacgcggtcg taaaagtaaa ggttggggta tccgatgaat tgctcaatgc ggcagctcaa
    31321 aacttcctta actggaaaac tgcagatatt tctgcctcta ttcaagatgt cttggaagga
    31381 aatctgcgtg aaatcattgg tcagatggaa ctgcgagaca tggtcaataa ccgccaggct
    31441 tttgcagaaa aagtccaatc caacgcagct cctgacttgg ctaaaatggg gctggaaatc
    31501 attgccttta cggttcaatc cttcacagat gacaacgatg tcattaaaaa cttgggtatt
    31561 gacaatattg tgaccatcca gaaggatgcc gcaaatgctc gcgccaaagc agagcgggaa
    31621 caagcagaag tccgtgcccg tgaggataag gcggctaatg atgcgcgtgt tgctgcagac
    31681 ttagaaatcg ccaagaaaca gaatgaacta gctattgagc aagctaatct aaaacgtcgg
    31741 tccgatgtcc aactggcaca ggcaaacgca gcctatggca ttgaggagca agctcaacgg
    31801 aaagaaatcg agcgtgcaac tgctgaagcc aatattgtca agcaacaaaa agaagcagaa
    31861 gtaaaagcag aggaagttaa ggtccgtgaa caagagctct cagctactat ccgcaaacag
    31921 gcagaggcag aaaaatacgc tcgccaacaa gctgcagaag cagatttgat tgaacgtcaa
    31981 cgcaaggcag aagcagaact ctacgaaacg cagcgtgaag cggaagctca aaaagcgcgt
    32041 gcagaagctg ccaagtatgc tgcggagcaa gaagcggcag gtattgaggc caaaggacgt
    32101 gccgaagcag aagccatccg tttgaaactc gaagccgagg cagaaggact cagcaagaag
    32161 gcggatgcca tggctaagtt taatgatgca gcggtgacag aaatggtggt taatgttctt
    32221 ccagaaatcg ccaagaatat tgcagcacca cttggaaatg ttgataagat taccatgtac
    32281 ggcgaaggga atgcctcaaa aatggtggga gacctgatga cgaccatgga caagacaaca
    32341 gaaggtctag gactcaatgt ccgtgattta atcagcgcaa ccctaactgg tcgtgccatg
    32401 tcaacaggtt tactgactgc ccaagaaaca aaagaagtgg aaggggacaa agaaaaataa
    32461 taagcagttg gaagagatgg gtgactgagt aggtttgcac catcatttcc atcgcatgat
    32521 tgtttcagaa atccctacga agatgaaaat gt
//
LOCUS       DBNFCK010000007        30818 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.007, whole genome shotgun sequence.
ACCESSION   DBNFCK010000007 DBNFCK010000000
VERSION     DBNFCK010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 30818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30818
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.007"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            62..616
                     /locus_tag="AC1PIW_000286"
     CDS             62..616
                     /locus_tag="AC1PIW_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009911037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIP6605255.1"
                     /translation="MNITLKKNLKTTIAGLVAGISLLTAGVVSAETYTVKSGDTLSEI
                     AETYNTTVEKLAEQNKITNLDFIHVGQVIELGDVTVKAVESSVEQTQSTSTSTATVTT
                     STYSSNLSAEDAAAKEWIAMKESSGSYDARNGIYIGRYQLTNTYLNGDYSPENQERVA
                     DAYVASRYGSWSAAKAFWLANGWY"
     gene            complement(1073..1945)
                     /gene="sdaAA"
                     /locus_tag="AC1PIW_000287"
     CDS             complement(1073..1945)
                     /gene="sdaAA"
                     /locus_tag="AC1PIW_000287"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HIP6605256.1"
                     /translation="MFYTIEELVQQADNFSGNVAELMIATEIELTGRSREEILTIMSR
                     NLTVMKASIVDGLTESKSVSGLTGGDAAKLDAYMKSGKTLSDSAVLSAARNAMAVNEL
                     NAKMGLVCATPTAGSAGCLPAVLGVAIEKLNLTEEQQLDFLFTAGAFGLVIANNASIS
                     GAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAICFVIKNMLGLICDPVAGLVEVP
                     CVRRNAMGASYAMVAADMALAGIESKIPVDEVINAMYQVGSALPTAFRETAEGGLAAT
                     PTGRRLAQEIFGED"
     gene            complement(1958..2629)
                     /gene="sdaAB"
                     /locus_tag="AC1PIW_000288"
     CDS             complement(1958..2629)
                     /gene="sdaAB"
                     /locus_tag="AC1PIW_000288"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HIP6605257.1"
                     /translation="MTNLKFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFGDEPT
                     EVEFQLYNSFAKTYRGHGTDVALVAGILGMDTDDPRIPDSLDIARERGIKVYWRVNKD
                     SNTPHPNTTRIIIKNDKKSISATGVSIGGGNIQVTELNGFSVNLNMNTPTIIIVHQDV
                     PGMIAKVTDVLSKYDINIAQMNVTREKAGEKAIMIIEVDARQCENSIAEIEKIPHLHN
                     VTFFN"
     gene            2844..3968
                     /gene="mnmA"
                     /locus_tag="AC1PIW_000289"
     CDS             2844..3968
                     /gene="mnmA"
                     /locus_tag="AC1PIW_000289"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIP6605258.1"
                     /translation="MSIDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDD
                     TDENGFCTATEDYKDVAAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDV
                     MCNKEIKFKAFLDYAMNLGADYVATGHYAQVTRDEDGTVHMLRGADNGKDQTYFLSQL
                     SQEQLQKTMFPLGHLQKPQVREIAERAGLATAKKKDSTGICFIGEKNFKEFLGQYLPA
                     QPGRMMTVDGRDMGEHTGLMYYTIGQRGGLGIGGQIGGDNEPWFVVGKDLSKNILYVG
                     QGFYHESLMSTSLQASQVHFTRDMPEEFTLECTAKFRYRQPDSQVTVKVKGDKAEVIF
                     AEPQRAITPGQAVVFYDGQECLGGGMIDMAYKDGEACQYI"
     gene            complement(4011..4400)
                     /locus_tag="AC1PIW_000290"
     CDS             complement(4011..4400)
                     /locus_tag="AC1PIW_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIP6605259.1"
                     /translation="MSTNLEIFNAYNNALIAGDFAALFETMADDIIWHQPGNHSTSGA
                     KIGKEVLGAHLATFAEKTNGTFKVVTNWVSDNDTLVAANVTFLGTRTDGEELNMNGID
                     LFRMEDGKIKEVWLFSADQKAEDAFWG"
     gene            4526..4852
                     /locus_tag="AC1PIW_000291"
     CDS             4526..4852
                     /locus_tag="AC1PIW_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIP6605260.1"
                     /translation="MNKVSEYGICPFATTQKVLTGKWGLVIIYQLSTGTKRFNELQRL
                     IPGITQTMLTRHLRQLEEDRIISRTVYAEVPPRVEYSLTEMGEKFRSVLDAVEAWGLE
                     YIETLT"
     gene            4994..5446
                     /locus_tag="AC1PIW_000292"
     CDS             4994..5446
                     /locus_tag="AC1PIW_000292"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIP6605261.1"
                     /translation="MDFRTRVDNQIFGVRATGLVVQDEKLYLVKSPEGKYYTLGGAIQ
                     LGETTEEAVQREMKEEIGIDVEVGPLAFIVENQFTLQEKSYHQIEFLYLVTPLSEPVT
                     YLEEGNSIRQCEWIAFVDLEKLDLNPAFLKTELAKWDGQLKHFMNKDN"
     gene            5463..5894
                     /locus_tag="AC1PIW_000293"
     CDS             5463..5894
                     /locus_tag="AC1PIW_000293"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIP6605262.1"
                     /translation="MPVKLIAHVLLTVADSHLMIQRSQIKRGEPNVFPLHWDIPGGRV
                     EENELPRDAAVRECFEETGISIEKENLTIIHEDSQFDEEKQTVFTRLVYEVTLPEQPK
                     TILLDPEEHTDFLWLAPSDKNKKNLVPYLDEILEKRRINGF"
     gene            5884..6345
                     /locus_tag="AC1PIW_000294"
     CDS             5884..6345
                     /locus_tag="AC1PIW_000294"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIP6605263.1"
                     /translation="MDFRTRIDNQIFGVRATALIIKDGKIFLTKDAKGRYYTIGGAVE
                     VNEVAADAAVREVKEELGIDSRVNQLVFVVENQFTHEGIDFHNIEFHFIVEPIGEMPK
                     EMIEGKLKQPCEWIALDNLVNLDVVPAFLTQELPNWSGQVKHIMNMKERTT"
     gene            6342..8258
                     /gene="mnmG"
                     /locus_tag="AC1PIW_000295"
     CDS             6342..8258
                     /gene="mnmG"
                     /locus_tag="AC1PIW_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HIP6605264.1"
                     /translation="MTHTFAENYDVIVIGAGHAGVEAGLAASRMGCKTLLATINLDMV
                     AFMPCNPSIGGSAKGIVVREIDALGGEMGRNIDKTYIQMKMLNMGKGPAVRALRAQAD
                     KAEYASEMKRTVERQENLTLRQTMIDEILVEDGKVIGVRTATNQKFSAKAVVVTTGTA
                     LRGEIIIGDLKYSSGPNNSLASITLADNLKELGLEIGRFKTGTPPRVNARTINYEDTE
                     IQPGDEKPNHFSFLSKDEDYLLDQIPCWLTYTNATSHEIINSNLHRAPMFSGIVKGIG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTDEIYVQGLSTSLPEDVQQDLIHSIKGL
                     ENAQMMRTGYAIEYDMVMPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIVAGI
                     NAALKVQGKPELILKRSDGYIGVMIDDLVTKGTVEPYRLLTSRAEYRLILRHDNADMR
                     LTEIGRQVGLVDDERWQVFQIHKNQFDNEMKRLESIKLKPIKETNEKVVAMGFKPLTD
                     ALTAKEFMRRPDVTYADAVAFIGPAAEDLDAKTIELIETEVKYEGYIAKALDQVEKMK
                     RMEEKRIPADIDWDDIDSIATEARQKFKLISPETIGQASRISGVNPADISILMVYLEG
                     RSRSISKNKSKDSH"
     gene            8365..10326
                     /locus_tag="AC1PIW_000296"
     CDS             8365..10326
                     /locus_tag="AC1PIW_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIP6605265.1"
                     /translation="MKRFRFSTIHFVMICVILFGLVALVHRFFPIGASTVFALLISLL
                     VLIALFIYQKHSYEFSELEQIEYLNNQANSGLMMLLDKMPVGVIKVRPDSNQVEWFNP
                     FAELIFAQENGEFDQKKLREIIDVGLDEERIYANFSGKRYAVNVDFDQGLFYFFDAST
                     EYSATNSLIGSRPVIGIISVDNYDELEDAVTDSQISQVNSFLAQFVSEFCHDYGIYYR
                     RGTMDRFYFFTDYAVLEKLIENKFSVIDKFREEAKKLDLGLTLSMGLAYGNENHHQIG
                     QVALQNLNMAEVRGGDQVVVKENDESKQPLFFGGGSASSVKRTRTRTRAMMTAVSDKL
                     KSVDAVFVVGHRNLDMDALGSAVGMQYFAQNIMNNAYTVYNPDEMAPDIARAIKKLSD
                     ENCSNLLTVEEAMKMVTFQSLLIMVDHSKIGLTLDKDFYEKFTQVVVVDHHRRDTDFP
                     NNAVLTYIESGASSASELVTELIQFQNDKHHKLNRIQSSLLMAGIMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSLEIQQIAATDFEEYRQINELILSGQRIESNVVIACADDTKEY
                     DNIIPSKAANTILDMAGIEAVFVVTRNIKGYVSISARSHNKINVQRIMEEMGGGGHFN
                     LAAAQVYDQTIAEVCEKLTDKIREEINGN"
     gene            10337..10789
                     /gene="rplI"
                     /locus_tag="AC1PIW_000297"
     CDS             10337..10789
                     /gene="rplI"
                     /locus_tag="AC1PIW_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIP6605266.1"
                     /translation="MKVIFLADVKGQGKKGEIKEVPTGYAQNFLIKKNLAKEATNQAI
                     SALRGQEKSKEKAHAEMIAEAEAIKAKLAEEATLVKFVEKVGPDGRTFGSITSKKIAE
                     ELDKQFGIKIDKRHIQLDHPIRAIGLIDVPVKIYQDVTGIINLSIKEA"
     gene            10821..12176
                     /gene="dnaB"
                     /locus_tag="AC1PIW_000298"
     CDS             10821..12176
                     /gene="dnaB"
                     /locus_tag="AC1PIW_000298"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIP6605267.1"
                     /translation="MAELDELRVQPQDILAEQSVLGAIFLDEGKLVFVREYIEAEDFF
                     KHAHKLIFKAMIALSDRNEAIDATTMRNYLVNHGDLENIGGLAYLAEIISSVPTAANA
                     EFYAKIVAEKSNLRKLIARLTDAVNQAYEGTDGSDDIIANAEKALIDVSEGASRSGFK
                     RIDDVLNINFDNLETRAKQTSDITGIATGYPALDAMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLGLTVAIFSLEMGAESLVDRMLASEGVINSRAIRTGHLTQEEWNKY
                     MVAQANLARASIYIDDTPGIKITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGEQEDGGIPNNTVEVIIEKNRSGARGTVELMFQKEYNKFASISKRED
                     G"
     gene            12180..12431
                     /locus_tag="AC1PIW_000299"
     CDS             12180..12431
                     /locus_tag="AC1PIW_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018164720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HIP6605268.1"
                     /translation="MSDAFTDVAKMKQIKQDIKDHEGQIVELTLENGRKREKNKQGRI
                     VEVYQSLFIVEFEDPNNTFVESYTYSDILTEKILIHYLD"
     gene            12739..13350
                     /gene="rpsD"
                     /locus_tag="AC1PIW_000300"
     CDS             12739..13350
                     /gene="rpsD"
                     /locus_tag="AC1PIW_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIP6605269.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFSYGLGEKQFRNLFVQATKIKQGTLGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVEVGQVISVREKSIKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            13416..13865
                     /locus_tag="AC1PIW_000301"
     CDS             13416..13865
                     /locus_tag="AC1PIW_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HIP6605270.1"
                     /translation="MWRKYFLLVLLAILVVGGAFFLSQSAATPNESDYVKAVKSYQAI
                     SIDEVEQKVQDEEEFILYIGRETCPYCRDFVPKLTEAVEQSHATIYYLDSESDPNGKI
                     QQFRQSQGLATVPSLTYYKSGKLSGILRKGSQATLSEIENFLALQGQ"
     gene            13998..15515
                     /locus_tag="AC1PIW_000302"
     CDS             13998..15515
                     /locus_tag="AC1PIW_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HIP6605271.1"
                     /translation="MKKKAWLGLLMSLLAVLFLVACGGKSEKTATSSSSTTSSSSEEA
                     VSGASEKVYTDPSELKDSYDVIVVGSGGAGMSAAISAKDAGASVALLEKMPVIGGNTA
                     KSSAGMNASQTKFQEAEGIADTNDKFYEETLKGGKGTNDPELLRYLVDNSASAIDWLD
                     GMGITLSNLTTTGGMSEKRTHRPADGSAVGGYLVNGLYHNLVEREVPIFVNADVTKLQ
                     DKDGAVTGVEVKIAGETKKISAKAVVLATGGFGADLEMVAKLNPALKGYVTTNQEGST
                     GDGIALAESEGAATVDMEQIQIHPSVEQETSYLITEAVRGEGAILVNSKGERFVNELE
                     TRDKVSAAINSLDPNYAYVIFDDALKDRVKAIAQYEEKGLVKTGATLADLAKEIGVPA
                     DALQTTLDTWNKAVADKNDAAFGRTSGMDHALNTGSYHAIKVAPGVHHTMGGVKINTN
                     TEVLKTDGSAISGLYAAGEVTGGVHGQNRIGGNAAADIIVFGRQAGEQAAGFAKK"
     gene            15601..16947
                     /locus_tag="AC1PIW_000303"
     CDS             15601..16947
                     /locus_tag="AC1PIW_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIP6605272.1"
                     /translation="MKKKIVLLLGAIAVIVLTWSAITGQRQQSYAEAELRDQDKIWII
                     TDLHYLSQDLFDDGEAFSYIEKTAAGKDLRYGKERMEALVEQVEREQPSLLLVSGDLT
                     LNGEKQSMVELAQYFTRIEEKGTEVLVIPGNHDIASGWARAFKGDQQIVTDQVTAQQF
                     AELFVNHGYQQASSRDKASLSYLAKPFSNAWFLMIDSNIYSDGYGKGAPPTNGCIKKE
                     TLEWIEVQLQAAKEAGVSLLPVVHHNVLQQHAMLSKGYTLDNAADLKALFDQYGIHFG
                     FSGHTHSQNIVKEDLGQVNYTEVVNGAFSIYPAIIGQLSLDDTSIHYQKTQLDMASWA
                     EKHQPSDPNLQDHVTYLQTVFDNTSDIMVHNVLNDERWYDGETADAISQFIMPANRAY
                     FSGEKLDQTWLDEKVFPSQAYQTLQAASPRSFLADYLDVIIKRSQSRDVEQVTVSK"
     gene            complement(16986..18293)
                     /locus_tag="AC1PIW_000304"
     CDS             complement(16986..18293)
                     /locus_tag="AC1PIW_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HIP6605273.1"
                     /translation="MINFPDIPRFYTALTESLACLLVCSSLVLSEKWSRKQVRILLIF
                     LIQILLQLAAGKLPLLLWTVGMGTNIAWMFVSVRFLGVIHKRTSLYLTAKAFIIAELV
                     ASITWHLYCLTIYQQPVDNLWTQGLFLLITSLLSYWLIYLQDQKVRLEELDKFIEQRD
                     VTVAVFTTLSIFVLSNIGFILSGTRQFQDSTSIFILRTTVNLSGLLLLFTQESQQYDR
                     YLRQELTSINNIFQLQYKQYQAYRENSEILDRKVHDLKHQLAIIQQESDKTKKEQYLE
                     EMSEVIQTLDAKIETGNPVLDTILSQKNYYCLQNGINFTCIVQGEPLHFMDVMDISAL
                     FGNAIDNAIEAVEKIDNPEQRLITLKVASHQQFLIIRLDNYDTSSLDLSTGQLPETSK
                     TNKDYHGFGLKSMEYVANKYGGSLTLSKEDNWVQLKIILPLQV"
     gene            complement(18290..19003)
                     /locus_tag="AC1PIW_000305"
     CDS             complement(18290..19003)
                     /locus_tag="AC1PIW_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIP6605274.1"
                     /translation="MKIAIVEDQKIEQDRLSNYIKTYCKKAGIAVEISCFNDGLHITS
                     DYQKQFDLIYLDVEMEIMDGMTTAQKIRNLDKEVLLVFVTNHSHVAIQGYSVEAIDFL
                     LKPLSSFVFEEHFKKILRKLPDSQEKQSLYIKSKQSTVKLYQEDIVYLESQGHQVHIY
                     TVDSGLTITHNSLKNLEAMLNSKLFVRAHSAYIINLSHVQKVEGSLVYVTNTALPISR
                     PRKKEFMTALTNFIGDSLL"
     gene            19242..22151
                     /locus_tag="AC1PIW_000306"
     CDS             19242..22151
                     /locus_tag="AC1PIW_000306"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HIP6605275.1"
                     /translation="MKTWKRKGALLSLAGALLSGKIVYADSYPSSYQSLDAVGIAKTV
                     FICSAVVVLGLLGFAIYRVVKEKSLSSKKKKLGFGLLALLSIIIVGANYAVNMFVNVI
                     DTYFAPSYADAAKIADAEEVSKSLVTTIQEEGTVLLENKNKTLPLEKSSKVNVFGISS
                     VAITYGGSGSGAADESKNINLQQGLENAGFEVNSELTDFYTENLPEKEGTNIFSLNGG
                     DYNIYEPTQDKYSEELLTNAKNFSDTALVVISRNGGEGADLPQNMAEYQQGDKDKHYL
                     ELQEVEKAMLKTVTENFDKVVVLVNSSNAMELGFLEDAGVDAALWIGGPGSTGLTGVA
                     NILAGEVNPSGRLVDTYAYDLESNPTYWNTGDFSYTNITYEKTPWGAKEPQTFNYKFV
                     NYQEGIYLGYRYYETRFVDNATGQVDEAAYDKVVQYPFGYGLSYTNFDQEITSMKENG
                     DKIEVNVKVTNTGDVAGKEVAQVYYTAPYTEGGVEKSHVVLAGFDKTEVLEPGKSEEL
                     TISFDRDDMASYDEKTEKAYVLDKGDYEIKLMNNAHDVLDSETYTVDSKEVLKQRSSD
                     KAEVTNAFEYAVGDINYVTRADWDGTLPTERTKDKEASEQIATEINNANFRIDESSET
                     PIDTVTTEDNGLRLVDLIGANYDDERWEQLVAQMSVDEMVNLVGFGGYATGAVKSIDK
                     PAVVDLDGPAGINGIFQGIKGIQYNSEVVVASTWNTDLAEEMGDAFAKEALAHGIVGL
                     YAPAVNIHRSPFSGRNFEYYSEDPLISGKMASSLVAKALENGVYTFTKHFALNDQEDN
                     REGVATWSNEQAMREIYLKAFEIPVKEGGATAIMSSFNRIGTLWAGGNSDLLNTVLRD
                     EWGFRGMVITDFDGQDYMSPDQAIRNGGDLMLTPVGDAPTATSTGTKEGVAALRQASK
                     NILYTVAHSAAFDIYKPKTKWWIVVLVASNIALIGLTGLGLVKLTGKKKEEKEVA"
     gene            22190..22669
                     /locus_tag="AC1PIW_000307"
     CDS             22190..22669
                     /locus_tag="AC1PIW_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HIP6605276.1"
                     /translation="MKEWIAKHPDLWEFIKFNVLSNIATITNFCVLWLSTNFLFTALS
                     SQPFHWFIFHYSVENGGLNGFLSFLLAYIAAQVVNYIVQRKLVFGAENDISKTLHWYI
                     LTVVVAGILSIVLPPYTTQLFTSWGLSLGWAQTAANWVNIFVQVAVNYPMMKFVIMK"
     gene            23031..25430
                     /locus_tag="AC1PIW_000308"
     CDS             23031..25430
                     /locus_tag="AC1PIW_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HIP6605277.1"
                     /translation="MKYQEIINRLTLEEKAALMSGKSVWETKDYPEKGIPSIFLSDGP
                     HGIRKQEGEGDHLGLNASVPATCFPTAATLANSWDVDLVERVGQGLGAEANSLGVNVI
                     LGPGLNIKRSPLCGRNFEYYSEDPYQAGKLAAAMIRGMQSQGVAATPKHLAVNSQELR
                     RMASDSIVDERTLRELYLTGFEIAVREGNPKAIMSAYNKINGVYANEDKHLLRDILRD
                     EWGFTGFVVSDWGGSNDHVLGVENGSHLEMPGTKKVGQKEIIHAVQSGRLSEQVLNER
                     VDELIDVVLELAHAEKQSVDYSEQHELARQAATESIVLLKNKDQILPLSSETKIALIG
                     EFAQNPRYQGAGSSLINTRQLDKTVDCIADYPLNVIDYEQGYQRVDKENRVLVDRAVN
                     LANQAELVLYYMGLDEMSESEGLDRHHISLPKNQLSLLNALVGTGKKIVVVLSAGSVV
                     DMNWDVDVDAVLHGYLSGEAGARAILDTLTGRVNPSGKLSETYPINLADVPSSDDYPA
                     EGDFSLYKEALYVGYRYFTSLDKTVKYPFGYGLSYTDFSYSQLDVTELGVRVTITNTG
                     LVDGAEIVQLYVGKEVRQIYRPAKELKGFKKVYVKAGESKQIFIPFEDYTFRYFNKET
                     GQFEVEAGNYLLYVGASSQDIRLVGEITREGTVDQFPASEQIPTYLTAQVQAVSEQDF
                     AQLLGRPVPEETWKIGQELSLNDPVLKLQFAKSGLARGVHKLLAGLLKKAEQKGTPDL
                     NLLFLYNMPFRAMAKMTGDMLDLPMVEGILTIVNGNFFKGLGQLWRAYQAKRKYQKQL
                     S"
     gene            25475..26488
                     /locus_tag="AC1PIW_000309"
     CDS             25475..26488
                     /locus_tag="AC1PIW_000309"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIP6605278.1"
                     /translation="MTLLSIAIPSYNSEAYLHYCVHSLVMGGDKVEILIINDGSTDRT
                     QEIAEGLENQFSNVRAIYQDNKGHGGAVNTGIREAKGKYFKVVDSDDWVDTRAYLKIL
                     ESLQALEDENTPVDAFISNFVYEKEGQSRKKSMSYQDVLPENRIFGWEDVDAFSKGQY
                     MMMHSLIYRTDLLREVGLVLPEHTFYVDNIFVFTPLQAVKTMFYLPVDFYRYFIGRND
                     QSVNESVMIKRIDQQLKVNRILVDNLDLEAIDNPDLRSYLLNHVEITTIISCALLNRA
                     GTAEHMMKKQELWHYIRDNNPALFKIVRKGLLGQLTNLYGYPGRKISNAVYKIAKRIY
                     GFN"
     gene            26558..27469
                     /locus_tag="AC1PIW_000310"
     CDS             26558..27469
                     /locus_tag="AC1PIW_000310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIP6605279.1"
                     /translation="MQRLGQTGLEVSRIALGCMRMASLREKETAKVLETVVEQRINFF
                     DHADIYGGGESEIRFAQGAKLAGLKREDMLLQSKCGIRKGYFDFSKEYILESVDGILK
                     RLDTEYLDVLVLHRPDALMEAEEVAEAFYLLKKAGKVHHFGVSNQNIYQMELLQSYLD
                     QPLAVNQLQLSPAHTPLIDAGLHVNMKDDAATMRDGGLIDYCQLKKITIQAWSPFLID
                     LQRGIFANYPDYAHLNQTIEEIAERYNVSHETIVVAWILRHPAKIQTIVGSMNPGRLT
                     KIAQASQITLTRPEWYEIYRSAGNILP"
     gene            27479..27931
                     /locus_tag="AC1PIW_000311"
     CDS             27479..27931
                     /locus_tag="AC1PIW_000311"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6605280.1"
                     /translation="MKWEIKTFEQLSLHELYTILTLRTDVFVVEQACPYPEIDGKDPS
                     CLHLLGTDNGELVAYLRILPAGLSYDEVSIGRVVIKPSHRGKGLGRPMMEQAIHYITT
                     EWKESQIKIGAQAYLEKFYQSLGFKPVSEVYLEDDIPHLDMLYSKPVN"
     gene            28026..29777
                     /gene="aspS"
                     /locus_tag="AC1PIW_000312"
     CDS             28026..29777
                     /gene="aspS"
                     /locus_tag="AC1PIW_000312"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIP6605281.1"
                     /translation="MKRSMYAGRVRKEHVGQEITLKGWVGRRRGLGGLIFIDLRDREG
                     IMQLVINPESVEAEVMAKAESLRSEFVIEVTGTVVEREQANDNIPTGAVELQVTSLTV
                     LNTAKTTPFEIKDGIEASDDTRLRYRYLDLRRPEMLNNFKLRAAVTHSIRNYLDDLEF
                     IDVETPMLTKSTPEGARDYLVPSRVSKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLNEVEIQDIVEGLIAKVLKDTKGIDVTLPFPRMAY
                     DHAMNFYGSDKPDTRFEMLLQDLTELVKEVDFKVFSEAPVVKAIVVKGAADSYSRKDI
                     DKLTDYAKQFGAKGLAWVKVDKGELAGPVAKFLTGITEKLTASLQLEDKDLVLFVADE
                     LEVANNTLGALRNRLAKEQGLIDESKFNFLWIVDWPMFEWSEEEGRYMSAHHPFTLPT
                     EETAHHLDGDLAQVRAVAYDIVLNGYELGGGSLRINQKDMQEQMFKALGFSAEDAHEQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEDNIREVIAFPKNNKASDPMTQAPSTV
                     ASAQLEELALDITLENE"
     gene            29764..30702
                     /locus_tag="AC1PIW_000313"
     CDS             29764..30702
                     /locus_tag="AC1PIW_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002265645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIP6605282.1"
                     /translation="MKMNKFFRKQRVRFLRKARKNKFWSVILGISREKYAERISGSII
                     YGLLSSIAVNFFFRPGNVYSSGVTGLAQIVSALAASYAGWNVPIAVVYYGLNIPLLIL
                     AWYKIGYKFTIFTFITVSFSSFFIQFMPPVTLTTDPLVNAIFGGLMLGTGIGFALRNN
                     VSSGGTDIVSIIIRKKTGRQVGSISLIVNIMIMLIAGMTFGWQYALYSMVALFISSRM
                     TDAIFVKQKRMQAMIITNHPERVIKKIHKKLNRGVTIINGAEGAYNHEQKTILITIIT
                     RAEFNDFKHIMKKADPKAFVSVADNVNIIGRFVESD"
BASE COUNT         9024 a         5747 c         7230 g         8817 t
ORIGIN      
        1 caaagtctaa gtaatagtta gagggtatga tagacacatg acagaaagga atgtgactat
       61 tatgaacatt acattaaaga aaaacttaaa aacaactatt gctggtttgg tagcgggaat
      121 ctccctttta actgcgggtg tagtcagtgc agaaacctat actgtaaagt ctggtgatac
      181 cctgtctgaa attgctgaaa catacaatac tactgttgaa aaattagcag agcagaataa
      241 gattaccaac ttagatttta tccatgtcgg tcaagttatc gaattgggtg atgtgacagt
      301 gaaggcagta gagagttcag ttgaacaaac tcaatcaaca tcaacttcta ccgcaacagt
      361 aacgactagc acctattctt caaatttgag tgctgaagat gcagcagcaa aagaatggat
      421 tgcaatgaag gaatcaagcg gtagctatga tgcccgtaac ggtatctaca ttggtcgtta
      481 ccagttgaca aatacttact tgaatggtga ttactcacca gaaaaccaag agcgtgtagc
      541 agatgcttat gttgctagtc gctatggttc atggtctgca gccaaggctt tctggttggc
      601 aaatggttgg tactaagata aattagaaga ggtctagcac gatgctagac tttttctgtc
      661 tcacgaatgt atttcacaac tgctttttcg ttcagtccaa cggtactgaa tagtagcctc
      721 tagcccaaaa cttttgattt ccacgctgta tggacatagg tgatggaaaa tatcaattaa
      781 gtcccgtcta attttttagt aaatggattt tctgcggtac ttaggagtga catacatttg
      841 gtatgtgata aactgtaacg gttttaacca tgctaaacta gtccactgga ctagtttact
      901 cctcctttat ttaacttgac aaattagatt ataacaggaa aaaaatagaa gctcaaagcc
      961 ttgctagcca ccagcatagg tagtagtttt cttgtttttc tctatgagaa aactagcccg
     1021 aagccataat aagaaaaagg accgttaggg tcctttttgg tttacattat tgtcaatctt
     1081 ctccaaaaat ttcttgagca aggcgacgac ccgtcggtgt agctgcaagg cctccttcgg
     1141 cagtttcacg gaaggcggtt ggtagggcag atcccacttg gtacatagcg ttgatgactt
     1201 catcaacagg gatttttgat tcaattcctg ccagtgccat atcggcagct accatggcgt
     1261 agcttgctcc cattgcatta cgtctgacac aagggacttc taccagacca gcgacgggat
     1321 cgcaaatcag acccagcata ttttttatga caaagcagat ggcttggctg gcttggtagg
     1381 gagttccacc tgctgccagt gtcagggctg cggcagacat agcagaggca gatccgactt
     1441 ctgcctgaca gcctccttct gcacctgaga tggaggcgtt gttggcgata actagtccaa
     1501 aggctccagc tgtaaatagg aagtcaagtt gctgttcttc tgtcaaattg agcttttcaa
     1561 tagcaactcc tagcacagct ggtagacagc ctgcagatcc tgctgtcgga gtggcacaga
     1621 ctaagcccat tttggcattc aattcattaa cggccatggc atttcttgcg gcggatagga
     1681 cagcggaatc tgacaaggtt ttaccagatt tcatataggc atctaacttg gcagcatcgc
     1741 ctcctgttaa accagatacg gatttgcttt cagtaagtcc atctacgatg gaggctttca
     1801 taactgtcaa gtttcttgac ataattgtca atatttcttc gcggctacga ccggtcaact
     1861 caatttcagt cgcgatcatg agttcagcta cattaccaga aaagttgtct gcttgctgaa
     1921 ctaattcttc gatagtataa aacatgtctt tctctttcta gttgaagaag gttacattgt
     1981 gcaggtgtgg aattttttca atttctgcaa tagaattttc acattggcga gcatccactt
     2041 cgataatcat aatggctttt tcgccagctt tttcacgtgt cacgttcatt tgggcgatat
     2101 tgatgtcata cttagaaaga acatcggtca ctttcgcaat cataccaggg acatcttggt
     2161 gaacaataat aatagttggc gtattcatat ttagattgac agagaagccg ttgagctctg
     2221 ttacttgaat gtttccccca ccgatggaaa cgcctgtggc tgagatggat tttttatcgt
     2281 tttttatgat gattctggtg gtattgggat gtggggtatt gctgtccttg ttgacacgcc
     2341 agtaaacctt aatgcctcgt tctcttgcaa tatcaagaga gtcaggaatg cggggatcat
     2401 ccgtatccat gcccagaata cctgctacca aggctacatc cgtaccgtga ccacgatagg
     2461 tcttggcaaa ggaattatag agttgaaatt ccacctctgt cggctcatcg ccgaagatgg
     2521 aagacacaat tttcccaatt cggaccgccc cagctgtgtg actagaagat ggaccaatca
     2581 taacaggtcc aataatatca aagaccgact gaaattttaa gtttgtcata gtaatcctca
     2641 cgtttttctt ggtactatta tatcaaattt tccagttaga gaatatgcct agatgaatgt
     2701 gttttcttat gttaaaattc tatcgcttac cctttttgta tggtataata caagttgttc
     2761 aaaatcgtca atcgtttttg gataatcaaa ttttggaata cgaccttcaa atcgttattt
     2821 ctacgaaaag atgggagaag attatgtcaa ttgacaattc taaaacccgt gttgttgtcg
     2881 gtatgagtgg cggtgtggat tcatcggtta cggctctctt gctcaaagag cagggctacg
     2941 atgtgatcgg catcttcatg aaaaactggg acgacacgga tgaaaatggc ttctgtacag
     3001 caacagaaga ttacaaggat gtggctgcag tggcagatca aatcggcatt ccttactact
     3061 ctgtcaactt tgaaaaagag tactgggacc gcgtatttga gtacttttta gcagagtatc
     3121 gggctggtcg cacgcccaat ccagatgtca tgtgtaacaa ggaaatcaag ttcaaggctt
     3181 tcttggatta cgctatgaac ttgggtgcgg actatgtggc gacagggcac tacgctcagg
     3241 ttacccgcga cgaggacggc accgttcata tgctgcgtgg ggcagataat ggtaaggacc
     3301 agacctattt ccttagccaa ctctcacagg aacagctgca gaaaaccatg tttccgctcg
     3361 gccatttaca aaagcctcag gtgagggaaa tagcagagcg agcaggcttg gcgaccgcta
     3421 aaaagaaaga ctcgacaggc atctgcttta tcggtgaaaa gaacttcaaa gaatttttag
     3481 ggcagtattt gccagcccag cccggtcgga tgatgaccgt tgacggtcgt gacatgggag
     3541 agcatacagg cctcatgtac tatacaatcg gtcagcgggg cgggcttggt atcggaggac
     3601 aaatcggagg ggacaatgag ccttggtttg ttgtcggaaa agacctgtca aaaaatatcc
     3661 tctatgtcgg tcaaggcttc tatcatgagt ccttgatgtc aacttcttta caggctagtc
     3721 aagttcactt tacacgtgat atgcctgaag aattcacact tgagtgtaca gctaagttcc
     3781 gctaccgtca accagacagt caagtgactg tcaaggtaaa aggtgacaag gcagaagtca
     3841 tctttgcaga gccacaacgg gctatcacac caggacaagc cgttgttttc tatgacggac
     3901 aggaatgcct cggtggcggc atgattgaca tggcctataa agacggagag gcttgtcagt
     3961 atatttaatc aaaaataata gcttagcact ctcgtgctaa gctatttatc ttatccccag
     4021 aaagcatctt cggctttttg gtcggctgag aagagccaaa cttccttgat tttgccatct
     4081 tccatacgga aaagatcgat accattcatg tttagttctt caccgtctgt gcgagtaccg
     4141 aggaatgtta cgttagcggc aacaagagta tcattatcgg atacccagtt tgtcactact
     4201 ttgaaagttc catttgtttt ttcggcgaaa gttgctagat gtgctccgag tacttcctta
     4261 ccaattttag caccagatgt tgaatgattt ccgggttgat gccagataat gtcgtctgcc
     4321 attgtttcaa aaagagctgc aaaatcacca gcgatgagag cattgttata ggcattaaaa
     4381 atttctaagt ttgttgacat ttgatgtcct ccaatttttt tgatatagta caattatagt
     4441 ggaagtagtc aattatacaa gtaggtactt ttgtatttgt tggtatcaaa agtgatacta
     4501 ttgtgataaa taaaggagaa tttttttgaa caaagttagt gaatatggta tttgtccttt
     4561 tgcaacaacg caaaaagtct tgactggtaa gtggggattg gtgattatct accaactcag
     4621 tacaggaacg aaacgcttca atgaattaca gcgactaatt ccaggtatta ctcaaaccat
     4681 gttaacaagg caccttagac aattggagga ggatcgcatt attagtcgca cggtctatgc
     4741 agaagtcccc cctcgtgtag agtatagctt gactgaaatg ggcgaaaaat ttcgttcggt
     4801 gctggatgct gttgaggctt ggggcttaga atatattgaa acgttaacgt aacaccagat
     4861 ttgaaattct acaaatcttt tgctaaaata agcgtaacaa ttatcatgat ggtcaaagct
     4921 catgggaatt gtaggctttt ttgtcctgca gttttcgaga gttttggcta ttttttgcga
     4981 ttggggagag aagatggatt tcagaaccag agttgacaac caaattttcg gtgtccgtgc
     5041 gacgggcttg gtggtacaag atgagaagtt atatctcgtt aagtcgcctg aagggaaata
     5101 ctacactttg ggtggagcta ttcagcttgg tgaaacaacc gaagaggctg tgcaacggga
     5161 aatgaaagaa gaaatcggaa ttgatgtgga ggttggacca ctagctttca tcgttgaaaa
     5221 tcagttcacc ttgcaggaga aatcctatca tcagattgaa tttctttatc ttgttacccc
     5281 actgtccgag ccagtcacct atctggaaga aggaaattcc attcgccagt gtgaatggat
     5341 tgcttttgtg gacttagaga aattggatct caatccagcc tttcttaaga ccgagctagc
     5401 caaatgggac ggacaactaa agcattttat gaacaaagac aactaaagta gcggaggaaa
     5461 ccatgcctgt taaattgatt gcccatgtct tgctgactgt tgcagacagt cacttgatga
     5521 ttcagcgttc gcagattaag cgtggagagc cgaatgtatt tccgctgcat tgggatattc
     5581 caggcggtcg tgtcgaggag aatgagttgc cccgtgatgc tgcagtgaga gagtgttttg
     5641 aggaaacagg tatttcgata gaaaaggaaa atctgaccat tatccatgaa gatagtcagt
     5701 ttgatgaaga aaaacagacg gtgtttacaa ggctggttta tgaagtaaca cttcctgaac
     5761 aaccgaaaac cattcttctt gacccagaag agcatacaga ttttctatgg ttagccccta
     5821 gcgataaaaa taagaaaaac ttagttccat atctggacga aattttagaa aagagaagga
     5881 taaatggatt ttaggaccag aattgacaat caaatcttcg gtgtccgtgc cactgcattg
     5941 attataaaag atgggaaaat atttctcacg aaggacgcta agggccgtta ttatacgatt
     6001 ggtggtgcgg ttgaggtcaa tgaggtagca gcggatgcgg ctgttcggga agtcaaagaa
     6061 gaattaggga ttgacagtcg tgtcaatcaa ttagtttttg tcgttgaaaa tcagtttaca
     6121 catgaaggta tagattttca caatatcgag tttcatttta tcgttgagcc tattggagaa
     6181 atgcctaagg agatgattga agggaagttg aaacaaccgt gtgaatggat tgcacttgac
     6241 aaccttgtca acttagatgt cgtaccagct tttctgacac aggaattacc aaactggagt
     6301 ggacaagtta aacatatcat gaacatgaag gagagaacaa catgacacac acatttgcag
     6361 aaaactatga tgtcatcgtg attggtgcgg ggcatgcggg tgtagaagcg ggattggcag
     6421 ccagtcggat gggctgtaag accctgcttg cgaccattaa cttggatatg gtggccttta
     6481 tgccttgtaa tccctccatt ggtggctctg ccaaggggat tgtggtaaga gaaatcgatg
     6541 ccctgggtgg cgaaatgggc cgcaacattg acaagaccta cattcagatg aaaatgctca
     6601 atatgggcaa ggggccagct gttcgtgcct tgcgggctca ggcagataag gcagagtatg
     6661 catcagagat gaagcggacg gtggagcgtc aggaaaatct gaccctgcgt caaaccatga
     6721 ttgatgagat tttggtggag gatggcaagg ttatcggtgt ccgcacggct accaaccaga
     6781 aattctcagc caaggcagtt gtggtgacaa cgggtacagc cttgcgtggt gagattatca
     6841 tcggtgacct caagtattcg tcagggccta acaatagcct tgcctctatc acgctggcgg
     6901 ataacctgaa agaacttgga cttgagatag gtcggttcaa gacaggaacg ccaccgcgtg
     6961 tcaatgctcg taccattaac tacgaagaca cggagattca accaggcgat gaaaagccaa
     7021 accacttttc attcttgtcc aaggacgagg actatttgct ggatcaaatt ccttgctggt
     7081 tgacctatac caacgccacc agccacgaga ttatcaacag caatcttcat cgagcaccta
     7141 tgttttcagg aattgtcaag gggattgggc cccgttattg tccgtcaatc gaggacaaga
     7201 ttgtccgttt tgcggataag gaacgccacc agcttttcct agagccagaa ggtcgcaata
     7261 cagacgaaat ctatgttcaa gggctgtcaa ccagtctgcc agaagatgtg cagcaggact
     7321 tgatccactc tattaaaggc ttggaaaatg cccagatgat gcggacgggc tatgctatcg
     7381 agtacgatat ggttatgccg caccagttgc gggcgacgct tgaaaccaag aagatttcag
     7441 ggctctttac agcaggtcag accaacggaa catcaggtta tgaagaagca gcaggtcagg
     7501 gaattgtcgc tggtatcaat gcagccctca aggtgcaggg caagcctgag ctgattttga
     7561 aacgaagcga cggctacatt ggtgttatga ttgatgacct ggtaaccaag ggaacggtgg
     7621 agccttaccg cctcttgacc agtcgggcag aataccgctt gattctgcgt catgacaatg
     7681 ctgatatgcg actgacggag attggtcgtc aggttggctt ggtagatgat gaacgctggc
     7741 aggtcttcca gattcacaaa aaccaatttg acaatgaaat gaagcgtttg gagtctatca
     7801 aactcaagcc aatcaaggaa accaacgaaa aagtcgtagc catgggcttc aagcccttga
     7861 cagacgctct gactgccaag gaattcatgc gtcgtccaga tgtgacttac gcagatgcgg
     7921 tagcctttat cggtcctgcg gcagaggatt tggacgccaa gaccattgaa ttgattgaaa
     7981 cagaagtcaa gtacgagggc tacattgcta aggccttaga tcaggtggag aagatgaaac
     8041 gtatggaaga aaaacgcatt ccagccgaca ttgactggga tgatattgac tccattgcga
     8101 ccgaggctcg ccaaaaattc aagctgattt caccagaaac cattggccag gctagtcgga
     8161 tttccggtgt caatccagcg gatatatcca ttctcatggt ctacttggaa ggacgcagtc
     8221 gttccatttc taaaaataaa tcaaaggaca gtcactaagg ctgttctttt cgctgtatgt
     8281 cagctatatg tcaactactt taaaatcatg tgaaaagtag cttttttact gaatttatgg
     8341 tagaatggca ggcagaggtt agtgatgaaa agatttcgat tttcgacaat tcactttgtc
     8401 atgatatgtg tcattttatt tgggttggtg gctcttgtcc accgattttt tccaattggt
     8461 gccagtacag tttttgcgct actcatcagc ctattggtgt tgattgcgct gtttatttat
     8521 caaaaacatt cttatgaatt tagtgagcta gaacagattg aatatctcaa taatcaggcc
     8581 aattctggtt tgatgatgct cttggataaa atgccagtgg gagttatcaa agttcgacca
     8641 gatagtaatc aggtagaatg gtttaatccc tttgctgagc tgatttttgc tcaagaaaat
     8701 ggtgagtttg atcaaaaaaa actacgtgaa attattgacg ttggtctgga tgaggagcga
     8761 atttatgcaa acttttcagg caagagatac gctgttaacg ttgattttga tcaagggtta
     8821 ttttactttt ttgatgcgtc gacagagtat tcagcgacaa atagtttgat cggaagccgt
     8881 cctgttatag ggattatttc agttgataac tatgacgagc ttgaggatgc tgtaactgat
     8941 tctcaaatta gccaagtcaa ttccttcttg gctcaatttg tttcagaatt ttgccatgat
     9001 tacggtatct actaccgacg cggcacaatg gatcgattct atttctttac agattatgct
     9061 gtgttggaaa aattgataga gaataagttt tcagttattg ataagttccg cgaggaggct
     9121 aaaaagttag atttaggttt gactttgagt atgggattgg cgtatggtaa tgaaaatcat
     9181 caccaaatcg gacaggttgc gctccaaaat ctcaatatgg ctgaggttcg tggtggagac
     9241 caggttgtgg tcaaggaaaa tgatgagagt aaacagccac ttttctttgg cggtggctcg
     9301 gcttcttccg ttaaacgaac acgtacgcgg actcgtgcta tgatgactgc tgtatcagat
     9361 aaactcaaat cggttgatgc tgtttttgtg gtgggacatc gtaatttaga tatggacgcg
     9421 cttggatccg cggtcggcat gcagtacttt gctcaaaata ttatgaacaa tgcttatacg
     9481 gtgtataatc cagatgaaat ggcaccagat attgcacgtg ctatcaagaa gttgagtgat
     9541 gaaaattgtt ctaatttgtt aacggttgag gaagccatga agatggtcac tttccagtcg
     9601 ttgttaatca tggtagatca ttcaaagatt ggcttgactt tggataagga tttctatgaa
     9661 aaatttaccc aggttgtggt ggttgatcat catcgtcgcg atacagattt cccaaataat
     9721 gcagttttga cttatataga gagtggtgct agttctgcga gtgagttagt aacggaatta
     9781 attcagttcc agaatgacaa acaccataag cttaatcgca ttcaatctag tttattgatg
     9841 gctgggatta tgcttgatac aaaaaatttc acctctcgtg tgactagtcg tacttttgat
     9901 gtggctagtt acttgcggac tcgtggtagc gatagcttgg aaatccagca gattgctgcg
     9961 acagattttg aggaatatcg tcaaatcaat gaactgattt taagtgggca gagaattgag
    10021 tccaatgttg ttattgcgtg tgcagatgat actaaagaat acgataatat tattccgagt
    10081 aaggctgcca atacaatatt ggacatggca ggaattgagg cggtattcgt tgtaacaaga
    10141 aatatcaaag ggtatgtatc tatttctgca aggagtcata ataaaatcaa tgttcaacgc
    10201 atcatggaag aaatgggtgg aggtggccac tttaacctag ctgccgctca ggtatatgac
    10261 cagacaatcg cagaagtatg tgaaaagttg acggataaaa tccgcgaaga aataaatgga
    10321 aattaggaga acagatatga aagtaatctt tttagcagat gttaaaggtc aaggtaaaaa
    10381 gggtgagatt aaggaagttc caacaggtta cgcccaaaat ttcttgatca agaaaaattt
    10441 ggctaaggaa gcaacaaacc aagcaatcag cgcccttcgt ggtcaggaaa aatctaagga
    10501 aaaggctcat gcagagatga ttgcagaagc agaggctatc aaggctaagt tggctgagga
    10561 agctacttta gtgaaatttg tcgaaaaagt tggtccagat ggtcgtacct ttggttctat
    10621 taccagcaag aaaattgctg aagaattgga caagcaattt ggcatcaaga ttgataaacg
    10681 ccatattcag ctggatcacc caattcgtgc gattggtctc attgatgtac cagttaaaat
    10741 ctatcaagat gtaacaggca tcattaattt gagtattaag gaagcttaat tatttatcta
    10801 gccagtgagg aggtgagatc ttggcagagt tggatgaatt gcgggtgcaa ccgcaggata
    10861 ttctagctga acaatcggta ttaggagcta ttttccttga tgaaggcaag ctggtcttcg
    10921 ttcgtgaata catcgaagct gaagattttt tcaaacatgc tcataagcta atttttaaag
    10981 ctatgattgc tctttctgat agaaatgagg caattgatgc gactaccatg cgtaactatt
    11041 tagtgaacca tggtgatttg gaaaatatcg gtggtttggc ctatttggct gaaatcatta
    11101 gttccgtacc gacggcagcc aatgcggaat tctatgccaa gattgttgct gaaaagtcta
    11161 atctccgtaa actgattgcc cgtttgacgg atgcagttaa tcaggcttat gagggtacgg
    11221 atggttcaga tgacattatt gccaatgctg aaaaagcttt gattgatgtc agtgagggag
    11281 ccagtcgaag tggtttcaag cggattgatg atgtcttgaa tatcaacttt gacaaccttg
    11341 aaactcgtgc taagcagacg tcggatatta cagggattgc aactggctat cctgctcttg
    11401 atgccatgac aacgggtctg catgaggaag agttgattat tcttgcggct cgtcctgcgg
    11461 ttggtaagac ggcttttgcc ctcaatattg ctcaaaatat cggtacaaaa ctgggtttga
    11521 cagttgctat cttctctttg gagatgggtg cagaaagctt ggttgaccgt atgctcgctt
    11581 ctgagggagt tatcaattcg agggctatcc gtacgggtca cctaacgcaa gaggaatgga
    11641 acaagtatat ggtggctcaa gctaatttgg ctcgagcaag tatctatatt gatgatactc
    11701 caggtatcaa gattaccgaa attcgctcgc gttctcgtaa attggcgcag gagacaggga
    11761 atttaggttt gattttgatt gactatctcc aattgattac aggaaccggt cgagaaaacc
    11821 gtcaacaaga agtttcagag atttctcgac aattgaaaat tctagccaag gaattaaaag
    11881 ttccagttat tgctctaagc cagttgtctc gtggtgtgga gcaacgtcaa gataaacgtc
    11941 ctgtcttgtc tgatatccgt gagtcagggt ctattgagca ggatgcggat atcgttgcct
    12001 tcttgtatcg tgatgattat tatgaacgag gagagcaaga agacggtggt attcccaata
    12061 atacggttga agttatcatc gagaaaaacc gttccggtgc gcgtggtacc gtggaactca
    12121 tgttccaaaa agaatacaat aaatttgcaa gtatttccaa gagggaggat ggatagagaa
    12181 tgagtgatgc atttacagat gttgctaaga tgaagcaaat caaacaagat attaaggacc
    12241 atgagggaca gattgtcgag ttgacccttg aaaatggtcg taagcgtgag aagaataagc
    12301 aggggcgtat tgtagaggtt tatcagtctc tcttcatcgt tgaatttgaa gatccaaaca
    12361 atacctttgt ggaatcttat acttactcag acattttgac tgaaaagatt ttgattcatt
    12421 atttggatta atcgctatat aaggctttac agagcctttt ttttgtgcta tactaatctt
    12481 tgtgtgaaat aacagcaggg caaaatgaag ctcgtcaaca gaaggtcagc aagaagagta
    12541 atcgttgctg tttcgatgaa ggcctcctgc acgaatcagg tttgcttaaa acgttatttc
    12601 ctacagataa gtctttttgt ttccttttag tactaggcaa ggaggtgcag gctatactga
    12661 ggtatgccaa gccgacttaa cgacgtaata aaagagaaac gaaatgacgg aaatttttat
    12721 tttaggagga cataacttat gtcacgttat acaggaccat cttggaaaca agctcgtcgt
    12781 cttggcttgt cattgacagg tacaggtaaa gaattggcac gtcgtaacta cgtaccaggt
    12841 caacacggac caaacaaccg ttctaaattg tcagaatacg gtttgcaatt ggctgagaaa
    12901 caaaaattgc gtttctctta cggtttgggt gaaaaacaat tccgtaactt gttcgtacaa
    12961 gctactaaaa tcaaacaagg tactcttggt ttcaacttca tgcttctttt ggagcgtcgt
    13021 ttggacaacg ttgtttaccg tcttggtttg gcaactactc gtcgtcaagc acgtcaattc
    13081 gtaaaccacg gtcatatcct tgttgacggc aaacgcgttg acatcccttc ataccgtgtt
    13141 gaagttggtc aagtgatctc agttcgtgag aagtcaatca aagttccagc tatccttgaa
    13201 gctgttgaag caactcttgg acgtccagct ttcgtatcat tcgatgctga aaagcttgaa
    13261 ggttcattga ctcgcttgcc agaacgcgac gaaatcaacc cagaaatcaa cgaagctctt
    13321 gtcgtagaat tctacaacaa aatgctctaa gatttgtatc gatattttaa aagccctatt
    13381 tacaagggct ttttgtctgt atttgaggag ggattatgtg gcgtaagtat tttttgctag
    13441 tgctattagc tattttggtc gtaggtgggg ccttctttct atctcaatca gcggctacgc
    13501 caaatgaatc agactatgtg aaggctgtaa agagttacca ggctatttcg atagacgaag
    13561 tggagcaaaa ggtccaagac gaggaagaat ttatattata cataggtcgt gagacatgcc
    13621 cttattgtcg tgattttgtt ccgaaattaa cagaggcagt agagcaatca catgcgacga
    13681 tctactattt agatagcgag tcggatccga atggaaaaat acaacaattt cgacagagtc
    13741 aaggtcttgc tacggttcct agcttaacct actataaatc aggtaaatta tctggaatac
    13801 tccgtaaagg gagccaagca acgctatccg aaatagagaa ttttttagct cttcaagggc
    13861 agtaaatagt tttaagaatg tatgaagcgg atatcataca tttttttgtt catgtttgtg
    13921 aaaatatttc aaaacaaatt gaaaaacatt tcacaatctt gtataatact aataccagat
    13981 aaaaattgga ggtttatatg aaaaagaaag cttggttagg cttattaatg agtctacttg
    14041 ctgttttgtt tttagtggcg tgtggaggta agtctgaaaa gacagctaca tcttcatcat
    14101 ctacgacttc ttcttcatca gaagaagctg tttcaggtgc atctgagaaa gtttatacag
    14161 atccatctga gttgaaagat agctacgatg ttatcgttgt tggttcaggt ggtgcaggta
    14221 tgtcagcagc tatctcagct aaagacgctg gtgctagtgt tgctctcttg gagaaaatgc
    14281 ctgttatcgg tggtaatacg gctaaatcat cagctggtat gaatgcttca cagactaaat
    14341 tccaagaagc ggaaggtatt gctgatacaa atgataaatt ctacgaagaa acgcttaaag
    14401 gtggtaaagg gacaaacgat cctgaattgc ttcgttatct tgtagataac tctgctagtg
    14461 caattgactg gttggatgga atggggatca ctcttagcaa cctaactaca actggtggta
    14521 tgagcgagaa acgtacccac cgtccagcag atggttcagc tgtcggtggc tacttggtaa
    14581 atggtcttta ccacaatctt gttgaacgtg aagtaccaat ttttgtcaat gcagatgtta
    14641 caaaactcca agacaaggat ggagctgtga caggtgttga agtgaagatt gctggtgaaa
    14701 ctaagaaaat ctcagctaaa gcagttgtat tggcgactgg tggtttcggt gctgatttgg
    14761 aaatggttgc taaattaaac ccagcattga aaggttatgt tacaactaac caagaaggat
    14821 caacaggtga cggtattgcg cttgctgaat cagaaggtgc agcaactgtt gatatggaac
    14881 aaatccaaat tcacccaagt gttgagcaag aaacttctta cttgattaca gaagcagttc
    14941 gtggcgaagg tgctatcctt gtcaactcaa aaggtgagcg ttttgttaac gaattggaaa
    15001 ctcgtgataa agtttctgcg gctatcaata gtttggatcc aaactatgct tatgttatct
    15061 ttgatgatgc cttgaaagat cgtgtgaagg ctattgctca atatgaagaa aaaggtcttg
    15121 taaaaacggg tgctactctt gcagatttgg ctaaagaaat tggtgtacct gcggatgctc
    15181 ttcaaacaac acttgatact tggaacaagg ctgtggcaga caaaaatgat gcggcatttg
    15241 gtcgtacatc aggtatggac catgctttga atactggttc ttaccatgcc attaaagttg
    15301 cacctggtgt tcaccataca atgggtggtg tgaaaatcaa taccaatact gaagtattga
    15361 aaacagacgg ttcagcaatt tctggtcttt atgctgctgg tgaagtaact ggtggtgttc
    15421 atggtcaaaa ccgtattggt ggtaatgcag cagcagacat catcgtattt ggtcgtcagg
    15481 ctggtgaaca agcagctgga tttgctaaga aataaattgt agacataaga ggctcggcag
    15541 aaagaaggtc gagcttcttt ttcttgtccc tctatctcta agatgatata cttttaaggt
    15601 atgaagaaga aaatagtatt attattagga gctattgcag ttatagtttt gacttggtcg
    15661 gctattactg gccagcgtca gcaatcctat gccgaggctg agttaagaga ccaggataaa
    15721 atctggatta tcactgattt gcattatttg tcacaggatt tatttgatga tggagaggct
    15781 ttttcctata tagaaaaaac agctgctggt aaggatttac gttatggaaa agagcggatg
    15841 gaggctttgg ttgagcaggt tgagcgtgag caaccgtcct tattactagt cagtggggat
    15901 ttgactctaa acggtgaaaa acagagcatg gttgagttgg cgcaatattt tacccggatt
    15961 gaagaaaaag gaacggaggt tttggtgatt cctggcaacc atgatattgc cagtggatgg
    16021 gctagagcct ttaagggaga ccagcagata gtgacggatc aggtcactgc tcagcaattt
    16081 gcagagctat ttgtcaatca cggttatcaa caagctagca gtcgtgacaa agcctcattg
    16141 agctacctgg ccaaaccatt ttccaatgct tggtttctga tgatagacag caacatctac
    16201 tctgacggct acggaaaagg agctcctcca accaatggat gtatcaaaaa agagacccta
    16261 gaatggatag aagttcagct tcaggcagcc aaggaagctg gagtgagcct gttacctgtt
    16321 gtccatcaca atgtcttgca acagcatgcc atgctctcaa aaggctatac tttggataat
    16381 gctgccgatt tgaaggcttt gtttgatcag tatgggattc attttggttt ttcaggccat
    16441 actcattctc agaatattgt taaggaagac ttgggacaag ttaactatac tgaagtggtc
    16501 aatggagcat tttctattta tccagctatt atcggtcaat tgagccttga tgatacaagc
    16561 attcactatc aaaaaactca acttgatatg gcttcgtggg cagaaaagca ccaacctagt
    16621 gatccaaatt tgcaggatca cgttacctac ttacaaactg tctttgacaa taccagtgac
    16681 atcatggtcc acaatgtgtt gaacgatgag cgttggtatg acggcgagac ggcagacgct
    16741 atttcacaat ttatcatgcc ggccaaccgt gcttattttt caggagaaaa actagatcag
    16801 acttggctag atgagaaggt atttccaagc caggcttatc agaccttgca agcagcatca
    16861 ccgagaagtt tcttggctga ttatcttgat gtgattataa aacggagtca aagtcgagat
    16921 gtggagcaag taacggtttc taaatgatga atagaaagaa aatcccgcct tcaagcggga
    16981 tttttctata cttgtaaagg gagaatgatc tttagttgaa cccaattatc ttccttgcta
    17041 agtgtgagac tgccaccgta tttattggca acatactcca tactcttaag accgaatccg
    17101 tggtagtcct tatttgtttt tgatgtttct gggagctgtc ctgtggataa gtcaagcgaa
    17161 gaagtatcgt agttgtctaa tcggatgatg agaaattgct gatggctggc aacctttaaa
    17221 gtaatcaagc gttgttcagg attgtctatt ttttccacag cctcgatggc attgtcaatg
    17281 gcatttccaa acagagctga aatatccatt acatccataa aatggagggg ttctccctga
    17341 acaatacagg taaagttgat tccattttgc aaacagtaat aatttttctg gctgagaatg
    17401 gtatctagaa cgggatttcc tgtttcaatc ttggcatcaa gtgtctggat cacctcgctc
    17461 atttcctcta ggtattgctc ctttttggtt ttatccgatt cttgctgaat gatggccagt
    17521 tgatgcttta aatcatggac cttacggtca agaatctcac tgttttcacg ataggcttgg
    17581 tattgcttgt attgaagttg aaagatatta ttgatagaag tcaattcctg ccgtaaatag
    17641 cgatcgtatt gttgagattc ttgggtgaat aggagtagaa gaccagagag attgacggtt
    17701 gtccggagaa tgaagatgct ggtcgaatct tgaaattggc gcgtgcctga gaggatgaaa
    17761 ccaatattac tgagaacaaa gatagataaa gttgtaaaga cggctaccgt cacatctcgc
    17821 tgctcaatga atttatccaa ttcttctaag cgaacttttt gatcttgaag atagatgagc
    17881 caataggaaa gtagagaagt tatcaacaga aataacccct gtgtccacaa gttatccaca
    17941 ggttgttgat aaatcgtcaa acagtagaga tgccaagtaa tggaggctac taattcggca
    18001 ataataaagg cttttgctgt caaatagaga ctagtcctct tgtggataac tccaagaaat
    18061 cgaacggaaa caaacatcca agcgatattg gtgcccattc ctacagtcca aaggagcaag
    18121 gggagtttgc cagccgctag ttgaagtaag atttgtatga gaaaaatcag gaggatacgt
    18181 acctgtttgc gtgaccattt ttcagacaaa acaagtgagg aacataccag taggcaggcc
    18241 aagctttctg ttagtgcagt atagaaacgt ggaatatcag ggaaattgat catagtaggc
    18301 tatctccgat gaagttggtt agagctgtca taaattcttt tttccttggg cggctaatcg
    18361 gcaaggctgt atttgtgacg taaacaaggc taccttccac tttttgaaca tgggacaaat
    18421 tgatgatata ggccgagtgg gctcgtacaa acaatttact gtttaacatg gcttcgagat
    18481 ttttgagact attatgggta atggttaggc cactgtcaac tgtgtagatg tgaacctgat
    18541 gtccttgact ttctaggtaa acaatatctt cttgatagag ttttacggtg gactgcttgc
    18601 tttttatata caaactttgc ttctcttggc tatcgggtaa ttttctaaga atttttttaa
    18661 aatgttcctc aaagacaaag gagctcagtg gtttgagaag aaaatcaatg gcctcaacag
    18721 agtagccctg aatagcgaca tgggaatgat tggtcacaaa tactaataga acttccttat
    18781 caagatttcg aattttttga gcggtggtca tcccgtccat aatctccatc tcgacatcta
    18841 ggtaaatcag gtcaaattgt ttttgatagt cactagtgat atggagaccg tcgttaaaac
    18901 agctgatttc cactgcaatc ccagcttttt tgcagtaggt tttgatgtag ttggacaatc
    18961 tatcttgctc aattttttga tcttctacaa tcgcgatttt cataaagcac cccttttatt
    19021 ttctatcagt atatcaaaat taaaatgaaa ttgttagcta tattttagaa aatcaaatga
    19081 taaaatagca agagtaggat tattcttata aatttacaaa ttatgaaggt taaatgacag
    19141 attatgaaga aagggttttc attgagtatg ttaattgata tagtggattc aagaaaaaga
    19201 aaaggcttac atgaaagccg aaatgtttag gaggaagtct tatgaagact tggaaaagga
    19261 aaggggctct cttgagccta gcaggagctc tgctatcagg aaaaatagtc tatgcagata
    19321 gctatccaag tagctatcaa tcattggatg cggtcgggat tgcaaaaaca gtatttatct
    19381 gttctgctgt agttgtcctt ggcttattgg gatttgcgat ttatcgcgta gtgaaggaaa
    19441 aatccctctc tagcaagaag aaaaagttgg gctttggcct cttggctctc ttgagtatca
    19501 ttatcgttgg agctaactat gctgtcaata tgtttgtcaa tgtgatcgac acctattttg
    19561 cgccgtctta tgcagatgct gctaaaattg ctgatgcaga agaagtttct aagtccttgg
    19621 taacgaccat tcaagaagaa ggcactgtct tgcttgaaaa caagaataag accctaccac
    19681 ttgaaaaatc atccaaggtc aatgtctttg gtatttcttc tgtagccatt acttacggtg
    19741 gatctggttc aggtgcggca gatgagtcca aaaacatcaa tctgcaacaa ggtcttgaaa
    19801 atgcaggttt tgaagtcaac agcgaattga cagacttcta cacagaaaac cttccagaaa
    19861 aagaagggac aaatattttc tccctcaatg gtggagatta caatatctac gagccaactc
    19921 aagacaaata ctcagaggaa ctgttaacaa acgcgaagaa cttttcagat acagccttgg
    19981 tagtcatttc tcgtaatggt ggtgaaggtg cggaccttcc acaaaacatg gcagaatacc
    20041 aacaaggtga caaggacaag cactaccttg aacttcaaga agttgaaaaa gccatgttga
    20101 agactgtgac agagaacttt gacaaggttg ttgtcttggt taactcctca aatgccatgg
    20161 aactcggctt cttagaagat gcaggcgtcg atgctgctct ttggattggt ggtcctggct
    20221 caaccggctt gactggtgta gctaatatct tggcagggga ggtcaaccca tctggtcgct
    20281 tggtcgatac ctatgcctac gacttggaaa gcaatccaac ctactggaat acaggtgact
    20341 tctcttatac taatatcacc tatgaaaaaa ctccatgggg agcaaaagaa ccacaaacct
    20401 ttaactacaa atttgttaac tatcaagaag gtatctatct aggctatcgt tactacgaaa
    20461 ctcgctttgt tgacaatgcg actggtcaag tggacgaagc agcttatgat aaagttgttc
    20521 aatacccatt tggttacggt ttgagctata ccaactttga ccaagaaatc actagcatga
    20581 aagaaaatgg tgacaagatt gaggtcaatg ttaaagtaac caatactggt gacgtggctg
    20641 gtaaggaagt tgcacaagtc tattacacag ctccatatac tgaaggtggt gttgagaaat
    20701 cacacgttgt cttggctgga tttgataaga ctgaggtcct tgagccaggt aagagcgaag
    20761 aattaaccat ttccttcgac cgtgatgata tggcatctta cgacgaaaag acagaaaaag
    20821 cctatgtttt ggacaagggt gactatgaaa tcaaactcat gaacaatgcg catgatgtct
    20881 tggattcaga aacctacaca gttgatagca aagaagtctt gaaacaacgt tcatctgata
    20941 aggctgaagt aaccaacgcc tttgaatatg ctgtgggcga tatcaactat gtaacccgtg
    21001 cggattggga cggtactttg ccaaccgaac gcacaaaaga caaagaagca agtgagcaaa
    21061 ttgctacaga aattaacaac gcaaacttcc gcattgacga atcatctgaa acaccgattg
    21121 atactgtgac aacagaagac aatggacttc gtttggttga tttgattggt gcaaactatg
    21181 atgatgagcg ttgggaacaa ctagttgctc aaatgtctgt tgatgaaatg gtcaacttgg
    21241 ttggctttgg tggttatgca acaggagcag tcaaatcaat cgataaacca gctgttgttg
    21301 acttggacgg acctgctggt atcaacggta tcttccaagg tatcaaaggt atccaataca
    21361 actcagaagt cgttgttgct tctacttgga ataccgactt ggcggaagaa atgggtgatg
    21421 cttttgctaa agaggcattg gcacacggta ttgttggtct atatgctcct gcagtgaaca
    21481 tccaccgctc accattctca ggccggaact tcgagtacta ttcagaagac ccactgattt
    21541 caggtaaaat ggcaagctca ctggtagcaa aagctcttga aaatggtgtc tatactttta
    21601 ccaaacactt tgctctcaat gaccaagaag ataaccgaga aggtgttgca acatggtcaa
    21661 atgaacaggc aatgcgtgaa atctatctga aagcctttga aattcctgtc aaagaaggcg
    21721 gagcgactgc tattatgtca tcctttaacc gtattggtac cctctgggca gggggtaata
    21781 gcgacctctt gaatacagtt ctccgtgacg agtggggctt cagaggaatg gttattacgg
    21841 actttgacgg tcaagactac atgagtccag atcaagcaat ccgcaatggt ggcgacctta
    21901 tgttaacccc agtaggtgat gcaccgacag caacctcaac aggtactaaa gaaggtgttg
    21961 cagcacttcg tcaggcaagt aagaacatct tgtacaccgt tgctcattct gcagcctttg
    22021 acatctataa accaaaaaca aaatggtgga ttgttgtatt ggtagcaagt aatattgccc
    22081 ttatcggctt gacaggtctt ggactcgtta agttgacagg taagaaaaaa gaagaaaaag
    22141 aagtagcata acaaggggga tttccccctt gtcttgcata ggagaccgta tgaaagaatg
    22201 gattgccaaa cacccagatt tgtgggagtt tatcaagttt aatgtcttat caaacatcgc
    22261 aactattaca aacttctgcg tcctttggtt gtcaaccaat ttcctcttta cagccttgtc
    22321 aagtcagcca tttcactggt ttatctttca ttattccgta gaaaatggcg gattgaacgg
    22381 ctttctctcc ttcctcttgg cttacatagc ggcacaggtt gtcaactata ttgttcaaag
    22441 gaagttggta ttcggagcag aaaacgatat atccaaaacc ctccattggt atatcttgac
    22501 tgttgtcgtt gcaggaattt tgtccattgt gctgccgcct tacaccactc aactctttac
    22561 atcatggggc ttgagcttgg gctgggcaca gacagcagcc aactgggtca atattttcgt
    22621 tcaagtggct gtcaactatc caatgatgaa gtttgtaatc atgaaataat actcttcgaa
    22681 aatcaaactc agccgttgtt ggcttgattt gatgagtgga aagttccagt ggaggtttcc
    22741 agcctgtcgc ttgacaataa taaagcgaca gagttctatc tgctcatata gaaacgaact
    22801 aggttcgtcc tatctcccac ctccaacagt ctattctcag actgttggag ctagcctgat
    22861 tttgattttc attgagtata agaagtaata ggagcgatct ccctagtatt ttctggggag
    22921 attttttata gcagattatg tccaaaatca tacagattat gaagaaagcg ttttaattgt
    22981 ccctttagtc tgatatgata gtgataaaga aatagaatga ggaaaagatg atgaagtatc
    23041 aggagattat taatcgttta acattggaag aaaaagcagc tctcatgtca ggaaaatctg
    23101 tttgggaaac aaaggattac cctgaaaaag gaattccatc tatctttttg tctgatggac
    23161 cacatggaat tcgcaagcaa gaaggtgaag gtgatcactt agggctgaat gcctctgtcc
    23221 ctgcaacttg tttcccaaca gctgctacct tggcaaattc ttgggatgtg gacctggttg
    23281 aacgtgtcgg acagggtctt ggcgcagaag caaatagctt gggtgtcaat gtcattttgg
    23341 ggccggggct taatatcaag cgcagtccct tatgtggtcg aaactttgag tattattcag
    23401 aggatccata tcaagcggga aaactggcgg ccgcaatgat ccgaggaatg cagtcgcaag
    23461 gtgttgcggc aacgccaaag catttagctg taaacagtca ggaattgcgc cgtatggctt
    23521 cagactccat tgtagatgag cgtacactga gagagctata tctgacagga tttgagattg
    23581 cagtgcgtga aggaaatcca aaggcaatca tgtctgccta taacaaaata aacggcgtct
    23641 acgctaatga agacaagcac ctcttacgtg acattctccg tgatgaatgg ggctttacag
    23701 gctttgtcgt atcggattgg ggtggctcca atgaccatgt tctcggtgtt gaaaatggta
    23761 gtcatttgga aatgccaggt actaaaaagg ttggtcaaaa ggaaatcatt cacgctgttc
    23821 agtctggtcg tctatccgag caagtattga atgagcgcgt ggatgagctg attgatgtag
    23881 ttttagagtt ggctcatgct gaaaaacaat ctgtagatta ttctgaacaa catgaattgg
    23941 cccgtcaggc tgccactgag agcattgtgc tattaaaaaa taaggatcag atcttgccct
    24001 tatctagtga gacaaaaata gctctcatcg gagaatttgc ccaaaatcct cgctatcagg
    24061 gagcaggttc ttctttaatc aatacaagac aattagataa aacagtggat tgcatcgcag
    24121 attatccatt aaatgtcatt gattacgaac aaggctatca acgtgtagat aaagaaaaca
    24181 gggtgttagt tgacagggct gtcaatcttg caaaccaagc ggaacttgtt ctttactata
    24241 tggggctaga tgaaatgagt gaaagtgagg gcttggatcg tcatcatata agtctcccta
    24301 aaaaccagtt atccttactg aatgccttgg tagggacagg taaaaaaatc gttgttgtcc
    24361 tatcagcagg ctcagttgtg gatatgaatt gggatgtgga tgtagatgcg gttcttcacg
    24421 gctatctatc aggagaagct ggtgccagag ccattttaga taccttgaca ggacgtgtca
    24481 acccatcagg aaaactaagc gaaacctatc ctattaatct tgcagatgtt ccatcttcgg
    24541 atgactatcc agcggaaggc gatttttccc tctataagga agctctctat gtaggctacc
    24601 gttactttac aagccttgac aagactgtca agtatccctt tggttatgga ttgtcctaca
    24661 cagacttttc ttacagtcaa ttggatgtga ccgaattagg agtcagagtc actattacaa
    24721 atactggtct ggttgatggt gctgaaattg tccaattgta cgtaggcaaa gaagttcgtc
    24781 agatttaccg ccctgcaaag gagttaaaag gctttaaaaa agtatatgta aaagcaggcg
    24841 agtccaaaca gatttttatt ccttttgagg actatacgtt tcgttatttt aataaagaga
    24901 caggccaatt tgaagtagag gctggaaatt atctcttgta tgtaggtgca agtagtcagg
    24961 atattcgctt agttggcgag attacccgag aaggcactgt agaccaattt cctgcttcag
    25021 aacagattcc gacttatctg acagcccaag tgcaggctgt ttcagagcag gattttgcac
    25081 aattgcttgg acgtccagtt ccagaagaaa cctggaaaat tggtcaagaa ctgagcctga
    25141 acgatccagt tttgaaactc caatttgcga agagtgggtt ggcacgtggc gtgcataaac
    25201 tgttggctgg cttactgaaa aaagcagagc agaaagggac tcctgacttg aacctgctgt
    25261 tcctttataa tatgccattt agggcaatgg caaagatgac aggagacatg ctcgatttac
    25321 cgatggtgga agggattcta accattgtca atggaaattt tttcaagggc ttaggccaac
    25381 tatggagagc ctatcaagca aaaagaaaat atcaaaagca actttcctga aaatgtgata
    25441 aaatagtaac aaattgtgtc aaatggagag aaagatgacc ttactaagca ttgccatacc
    25501 gagctataat tcggaagcct accttcatta ctgtgttcat tcccttgtaa tgggcggtga
    25561 caaggttgaa attctgatta ttaacgacgg gtcgaccgat cggactcagg aaatcgcaga
    25621 ggggctagag aatcaatttt caaacgtacg tgccatttat caggacaata agggccatgg
    25681 cggagctgtt aatacaggga tacgtgaggc caaggggaag tactttaaag ttgtagattc
    25741 agatgactgg gtcgatacca gggcctattt gaagatccta gagagtttgc aggctttgga
    25801 agatgaaaat acgccagttg atgcctttat ctccaacttt gtttacgaaa aagagggaca
    25861 atctcgtaaa aaatccatgt cttatcagga tgttttacca gaaaatcgga tttttggctg
    25921 ggaagatgtg gatgcctttt ccaaggggca atacatgatg atgcattcct tgatttacag
    25981 gacagattta ttgcgtgagg ttggtctcgt tctgccagag cataccttct atgtggataa
    26041 tattttcgta tttactcctt tgcaggctgt gaaaactatg ttctatctgc cagtagattt
    26101 ttatcgttac tttattgggc gaaatgacca gtctgtcaat gaatccgtca tgattaagcg
    26161 cattgaccag cagttaaaag tcaatcgaat tttggtagat aatttagact tggaggcgat
    26221 tgacaatcct gatttacgaa gttatttgct caatcatgtg gagattacga ccattatctc
    26281 ctgtgctctt ctcaatcgtg ctggaacagc tgaacatatg atgaaaaagc aggaactctg
    26341 gcactatatt cgggataata atccagctct atttaaaatc gttcgaaaag ggttactcgg
    26401 gcaattaacc aacctttacg gttacccagg tcgaaaaatt tccaatgccg tctataaaat
    26461 cgcgaaaaga atttatggtt ttaactaaaa ctcagtttcg gctgagtttt tctgttataa
    26521 taaggaatat gaaaagcgtt tacaaaggag aagataaatg caaagattag gacaaacagg
    26581 tctagaagtt tcacgaattg ctttaggctg tatgcgaatg gcaagtttga gagagaagga
    26641 aacagccaag gtattggaaa ccgtggttga gcaaagaatc aacttttttg atcatgcgga
    26701 tatttatggt ggtggtgaat cagaaatccg ttttgctcag ggggcaaaat tggcgggatt
    26761 gaaacgtgag gacatgctcc tccaatcaaa atgtggtatt cgtaaaggct attttgattt
    26821 ctcaaaagag tatattttgg aatctgtaga tggaattttg aagcgcttgg atacggaata
    26881 tttagatgtt ctcgtcttac atcgtccgga tgctctaatg gaagcagagg aagtagctga
    26941 agccttttat ctcttgaaaa aagcagggaa ggttcatcat tttggcgtca gcaaccaaaa
    27001 catttaccaa atggagttat tacaatccta tctcgatcaa ccgcttgcag ttaatcaatt
    27061 gcagctatct cctgctcaca cgcctttgat tgatgctgga ttacatgtca atatgaaaga
    27121 tgatgctgcg actatgcgtg atggtgggtt aattgactat tgccagctta aaaaaatcac
    27181 tatccaagcc tggtctcctt tcttgattga tttgcaacga ggtatttttg ccaactatcc
    27241 tgactatgct catttaaatc aaactattga ggaaatagca gagcgataca atgtttcaca
    27301 tgaaacaatt gtcgtagctt ggattttgcg tcatccagca aagattcaaa cgattgtagg
    27361 ttctatgaat ccaggtcgcc tgacaaagat tgcccaggct agccagatta ccttaacgag
    27421 accagaatgg tatgagattt acagaagtgc agggaacatt ctgccctagg aggaatgtat
    27481 gaagtgggaa ataaaaacat ttgaacaact tagtttacat gaactttaca ctatcttgac
    27541 acttcggacc gatgtgtttg tcgtggaaca agcctgtccc tatccagaaa tagatggaaa
    27601 agatccaagt tgtcttcatc ttttaggaac agacaatggg gagttagtgg cttatttgag
    27661 aatcttgcca gctggtctaa gttatgatga agtttccatc ggacgtgtgg tcatcaagcc
    27721 aagtcatcgt ggcaagggtt taggtaggcc aatgatggag caagctatcc attacattac
    27781 aaccgagtgg aaggaaagtc agattaaaat cggtgcccaa gcctatttgg aaaaattcta
    27841 tcaatcgctt ggctttaagc cagtttctga agtatatttg gaggatgata ttccgcattt
    27901 agatatgtta tatagtaaac cagtcaacta gggctggttt ttcgattttt ctcggcaatt
    27961 tctccccaaa atcagctatt ttatggtaga atgaataggt ataaaatcag taaaagagga
    28021 agaaaatgaa acgttctatg tacgctggac gtgttcgtaa agaacatgtc ggacaggaaa
    28081 ttactttgaa aggttgggtt ggccgccgtc gtggcttggg tggcctgatt ttcattgact
    28141 tgcgtgaccg tgaaggcatt atgcaattgg tgattaaccc tgagtcagtt gaagctgaag
    28201 tgatggcaaa agcggagagt cttcgttccg agtttgttat cgaagtaaca ggaacggttg
    28261 tggagcgtga gcaagctaac gacaatatcc cgacaggagc tgttgaactg caagtaacca
    28321 gcttgacagt gttgaacacg gctaagacaa ctccgtttga aatcaaggac ggcattgaag
    28381 ccagtgatga tacccgtctt cgttaccgtt atttggatct tcgccgtcca gaaatgctca
    28441 acaacttcaa attacgtgcg gctgtaaccc acagcattcg caactacttg gacgatttgg
    28501 aatttatcga tgtggaaaca ccaatgttga ccaagtcaac accagaaggt gcgcgcgact
    28561 acttggtacc atctcgtgtg tccaaaggtc atttctatgc tttgccacaa agtccacaga
    28621 ttactaagca gcttttaatg aacgctggtt ttgaccgtta ctaccaaatc gttaagtgtt
    28681 tccgtgatga agatttgcgt ggggatcgtc aacctgagtt tacacaggtt gacttggaaa
    28741 cctcattctt gaatgaggtt gaaatccaag acatcgtaga aggcttgatt gctaaggtct
    28801 tgaaagatac caaaggaatt gatgtgacct tgccattccc acgtatggcc tatgaccatg
    28861 cgatgaactt ctacggttca gataaaccag atactcgttt tgagatgctt ttgcaagact
    28921 tgacagaact tgtcaaggaa gttgacttca aggtcttctc agaagcacca gttgtcaaag
    28981 ccattgtggt caagggtgcg gcggatagct actcacgtaa agacattgat aaattaacag
    29041 actacgcaaa acaatttggt gctaagggac ttgcttgggt taaagtagac aagggagaat
    29101 tagcgggccc agttgctaag ttcttgacgg gtattacaga aaaattgaca gcaagtttac
    29161 agttggaaga caaggatttg gttctctttg tagcagatga attggaagta gccaacaata
    29221 ccctgggagc cttgcgtaac cgcttggcaa aagagcaagg cttgattgat gaaagcaagt
    29281 tcaacttcct ctggattgtg gattggccaa tgtttgagtg gtctgaagag gaaggtcgct
    29341 atatgagtgc ccatcaccca ttcaccttgc cgacggaaga aacagctcat catttggatg
    29401 gtgatttggc gcaggtgcgt gcagttgcct acgatattgt tttgaatggt tatgaattgg
    29461 gtggcggtag ccttcgtatc aaccaaaaag acatgcaaga acagatgttc aaggctcttg
    29521 gtttctcagc tgaagatgcc catgagcagt ttggtttctt gctagaagct atggactacg
    29581 gtttcccacc acacggtgga ttggctatcg ggttggaccg ctttgtcatg ttattggctg
    29641 gtgaggataa cattcgtgaa gttatcgcct ttccgaagaa taacaaggca tctgatccga
    29701 tgacacaggc accaagtacg gttgcatcag ctcaattaga ggaattggct ttagacatta
    29761 cacttgaaaa tgaataaatt tttcagaaaa cagcgtgtac gctttttaag aaaggccaga
    29821 aagaataagt tttggtcggt cattttggga atttctcggg aaaaatatgc tgaacggata
    29881 tcggggtcca ttatttatgg actcctatct agtattgcag tcaatttttt ctttcgacca
    29941 gggaatgtct attcgtctgg agttactggt cttgcacaga ttgtttctgc tctggcagct
    30001 tcttacgctg gttggaatgt accgattgcg gtggtctact acggcctgaa cattcccttg
    30061 cttatcttag cttggtataa aattggttat aaatttacta tctttacctt tattacggta
    30121 tccttcagtt ctttctttat ccaatttatg ccgccagtta ccctgacgac ggatccgtta
    30181 gtcaatgcta ttttcggtgg tctcatgctg ggaactggga ttggctttgc acttaggaac
    30241 aatgtatcca gtggtggtac tgatattgtc tctattatta ttcgcaagaa gacgggacga
    30301 caagtcggct ctatttcctt gattgtcaac attatgatta tgttgattgc agggatgacc
    30361 tttggctggc aatatgccct gtattcaatg gttgcgctgt ttatctccag tcgcatgaca
    30421 gatgcgattt tcgtgaaaca aaaacggatg caggccatga ttattaccaa tcatcctgaa
    30481 cgtgtcatta agaaaatcca taagaagctc aatcgcggtg tgaccattat caacggtgct
    30541 gagggtgctt ataaccatga gcaaaagacg attctcatta ccatcatcac tcgtgcggag
    30601 tttaatgatt tcaagcatat tatgaaaaag gcagacccca aagcctttgt atcggtagct
    30661 gacaacgtca atattattgg tcggtttgtg gaaagcgact gattgacaaa cactacatgt
    30721 aggagtaaaa tagtatttac agtctacatg tagtgttttt attataaaga ggaagtgtat
    30781 gaagaagaaa atatatttgg tttacatcag tttgagtg
//
LOCUS       DBNFCK010000008        30559 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.008, whole genome shotgun sequence.
ACCESSION   DBNFCK010000008 DBNFCK010000000
VERSION     DBNFCK010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 30559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30559
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.008"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(99..518)
                     /locus_tag="AC1PIW_000314"
     CDS             complement(99..518)
                     /locus_tag="AC1PIW_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HIP6605283.1"
                     /translation="MQISSRFTIASHILVLLALEGEKEKQTSTSIAGSVGVNPVIIRN
                     ILSQLKEAGLVEVARGIGGARLAQGPDQITLLHVYQAVELFGEKGQLFAFHEQPNPSC
                     QVGRNIHPLLDSRLENAQSALENELAKTRLADLLAEL"
     gene            complement(561..1628)
                     /gene="dinB"
                     /locus_tag="AC1PIW_000315"
     CDS             complement(561..1628)
                     /gene="dinB"
                     /locus_tag="AC1PIW_000315"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HIP6605284.1"
                     /translation="MLIFPLINDLSRKIIHVDMDAFFAAIEVRDSPALKGKPVIIGAD
                     PRLSGGRGVVSTCNYEARAFGIHSAMSSKEAYERCPQAIFISGNYEKYQEVGRQVREI
                     FHRYTDLVEPMSIDEAYLDVTENKLGISSAVKIAKLIQYDIWNELHLTASAGVSYNKF
                     LAKIASDMEKPHGLTLILPEDAVGILASLPVEKFHGVGKKTVERLHEMGVYTGQDLLD
                     VPEMVLIDRFGRFGFDLYRKARGISNSPVKVDRVRKSSGKERTYRKLLYREEDVLKEL
                     ISLCQRVAASLKRNGKKGRTIVLKVRYGDFSTLTKRHSLEVYTDQTETIEKEVRQLIE
                     EIGKIEKGIRLLGVTVTNFQT"
     gene            1944..4289
                     /gene="pflB"
                     /locus_tag="AC1PIW_000316"
     CDS             1944..4289
                     /gene="pflB"
                     /locus_tag="AC1PIW_000316"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HIP6605285.1"
                     /translation="MSTKVKTKNVAEDIFAQAWEGFKGTDWQDKASVTHFVQANYTPY
                     DGDESFLAGPTERSLHIKKIIEETKAGYEDTRFPMDVDRATSIADIPAGFIDKENELI
                     FGIQNDELFKLNFMPRGGIRMAETTLIENGYTPDPLLHEIYTKHATTVNDGIFRAYTA
                     DIRRARHSHHVSGLPDAYSRGRIIGMYARLALYGADYLMEEKVADWNAITEIDEESIR
                     LREEINMQYQALQQVVRLGDHYGVDVRKPAMNTKEAIQWTNIAFMAVCRVINGAATSL
                     GRVPIVLDIYAERDLARGTFTEAEIQEFVDDFVLKLRTVKFARTKAFDEIYSGDPTFL
                     TTSMAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWSDQLPYSFRRYCMAMSH
                     KHSSIQYEGVTTMAKDGYGEMSCISCCVSPLDPESEDQRHNIQYFGARVNVLKALLSS
                     WNNGYDDVHKDYKVFDGVEPNTSEVFDYDQVVKNFEIALDWLTDTYVDAMNIIHYMTD
                     KYNYEAVQMAFLPTHLRANMGFGICGFANTVDSLSAIKYAQVKPIRDEDGFIYDYEVT
                     GDFPRYGEDDDRVDDIAKWLMEAFFTRLNKHKLYKNAEATVSILTITSNVAYSKQTGN
                     SPVHRGVFLNEDGSVNTTKVEFFPPGANPTSKARGGWLQNLNTLSKLNFKHANDGISL
                     TTQVSPKALGKTFDEQVNNLVTILDGYFEQGGQHVNLNVMDLNDVYEKIMAGEDVIVR
                     ISGYCVNTKYLTKEQKTELTQRVFHEVLSMDDHAAEVSGQA"
     gene            complement(4696..5642)
                     /locus_tag="AC1PIW_000317"
                     /pseudo
     CDS             complement(4696..5642)
                     /locus_tag="AC1PIW_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941482.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
     gene            complement(5639..6385)
                     /locus_tag="AC1PIW_000318"
     CDS             complement(5639..6385)
                     /locus_tag="AC1PIW_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIP6605286.1"
                     /translation="MKCIVLRKNEAIVPILRINNRAINQGFLEENLGMKTKLEDGPFA
                     DFGDRTSPEAKLVLTESPGNRTRAVEGLKKLNKIVIKVDNASEIEALLAQGSQYSKLY
                     QGKNGYGFEALSPEGDTFLLHAEASVDDLKAILPPVPFKVQDDFSGLTAFTVESVWIN
                     TPQASISQDYYEAILPQQAFLRFVGAEGKDLLTPAEQVWDLEGLRFPVEQDFDWAHLE
                     SRLERPFFKDKKASFIQTVDPSGIELWFEK"
     gene            6555..8822
                     /locus_tag="AC1PIW_000319"
     CDS             6555..8822
                     /locus_tag="AC1PIW_000319"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HIP6605287.1"
                     /translation="MRFNQFSFIKKETSVYLQELDTLGFQLIPDASSKTNLETFVRKC
                     HFLTANTDFALSNMIAEWDTDLLTFFQSDRELTDQIFYQVAFQLLGFVPGMDYTDVMD
                     FVEKSNFPIVYGDIIDNLYQLLNTRTKSGNTLIDQLVSDDLIPEDNHYHFFNGKSMAT
                     FSTKNLIREVVYVETPVDTAGTGQTDIVKLSILRPHFDGKIPAVITNSPYHQGVNDVA
                     SDKALHKMEGELAEKQVGTIQVKQASITKLDLDQRNLPVSPATEKLGHITSYSLNDYF
                     LARGFASLHVSGVGTLGSTGYMTSGDYQQVEGYKAVIDWLNGRTKAYTDHTRSLEVKA
                     DWPNGKVATTGLSYLGTMSNALATTGVDGLEVIIAEAGISSWYDYYRENGLVTSPGGY
                     PGEDLDSLTALTYSKSLQAGDFLRNKAAYEKGLAAERAALDRTSGDYNQYWHDRNYLL
                     HADRVKCEVVFTHGSQDWNVKPIHVWNMFHALPSHIKKHLFFHNGAHVYMNNWQSIDF
                     RESMNALLSQKLLGYENNYQLPTVIWQDNSGEQTWTTLDTFGGENETVLPLGTGSQTV
                     ANQYTQEDFERYGKSYSAFHQDLYAGKANQISIELPVTEGLLLNGQVTLKLRVASSVA
                     KGLLSAQLLDKGNKKRLAPIPAPKARLSLDNGRYHAQENLVELPYVEMPQRLVTKGFM
                     NLQNRTDLMTVEEVVPGQWMNLTWKLQPTIYQLKKGDVLELILYTTDFECTVRDNSQW
                     QIHLDLSQSQLILPH"
     gene            complement(9099..10784)
                     /locus_tag="AC1PIW_000320"
     CDS             complement(9099..10784)
                     /locus_tag="AC1PIW_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HIP6605288.1"
                     /translation="METANKKFRYSIRKFKVGVGSVLIATCLLGAGVSTPTAFATTET
                     STATQVEAMAKVEEVQKLVKELEKELGELDKVPSYGDAQDYSYQKALWEEFLRIGKDN
                     MDYASKMKADDKFFHKVKGDLNDFKYQIKVENYIRQVAELRKKYPGDNTIEEEYNAHL
                     KQDEGKSIASQEGATLRDYVDREASEAMGRIKQRVAELEKSKQPQPSPADEPAPAPKE
                     EETPAPKEEDTPAPDAAPAPAPTPEVDPAPTPIPDTPKAEEEAPTPVPDTPAPKEDEV
                     PAPIPDAPTPKVEEETQEPKTEEKAPETKEETPTPVPDTPAPKEDEVPAPMPDAPAPK
                     AEEEVPAPTPMPETPMDKPKTDKVESDKQMPEAKQPEMEQPKAEDMPKEEMPKSEQPK
                     AEDSAPKTAVPEVAPKTAEKPKLDFTTKERKVEEALPIKEEIRYDASLPLGKSYLLQE
                     GKAGKKVSVYQDVIVDGKVVATNLLSETVVEGQNRILVKGSLEMKKEEVKTTPSVQSN
                     PTLSHKGAPSANKATLPATGEQRNNLALVGLGLAGISLAVVATAINKKSKDQI"
     gene            complement(10914..12083)
                     /locus_tag="AC1PIW_000321"
     CDS             complement(10914..12083)
                     /locus_tag="AC1PIW_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIP6605289.1"
                     /translation="MFRLAKEELEKLGAIITATEIYQQPGLWKEAYQLYFDQLEKIES
                     FLKSIKEKHDFVHVVFTGAGTSDFVGQSIANYLNQVNDLKHIRFSAIGTVELVSRPHD
                     YLQADVPTVLVSFARSGNSPESVAAVEVAKQLVDKLYQVTITCAPEGKLALAAEGDAE
                     NLLLLQPAGSNDKGFAMTGSYSCMALTALLVFSSASQEEKAAWVEIIARLGQDVLDRE
                     DYVQDLINLDFERVIYLGSGGFYGLAHEAQLKILELTAGKIATMYESPLGFRHGPKSF
                     INEKTLIFLFASNDAYTRQYDVDLLNEVHGDGIAERIVALSADKLVGTEAANFVLAEG
                     GADLPDVFLTFPYILFAQTVSIMAAIKCNNLPDTPSPTGTVNRVVQGVIIHPLEK"
     gene            12350..12649
                     /locus_tag="AC1PIW_000322"
     CDS             12350..12649
                     /locus_tag="AC1PIW_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIP6605290.1"
                     /translation="MMNMQNMMRQAQKLQKQMEKSQAELAATQFTGSSVQDLVTATFT
                     GDKKLVSIDFKADVVDADDLETLQEMTIQAVNAALTKVDEATQKKLGAFAGKLPF"
     gene            complement(12693..14453)
                     /locus_tag="AC1PIW_000323"
     CDS             complement(12693..14453)
                     /locus_tag="AC1PIW_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIP6605291.1"
                     /translation="MKKIQREFQKIYYNLDKILLLFFTLFTFMEFVWIPLNSWISEGL
                     LAMTGHAYLSPTNLLSVFAENLLVTGLFILLFFVNIAIAYLELALLFTGVWQLLDEKV
                     KHLTDYLRDVRDSMVAIIRHSSLPKVLFLLFYSVILLPFLRRVLNIYYFNKIVVPQFI
                     VDYLSNTVWLGILILIFLLLFFWLAARFMYALPNIYFEHRGVKESIAYSWEKTRGRKQ
                     IGSLFRLIWLVTFPVLLFTGVGGLFYLVQLLAEHFVPSASYWLAVVCYALLKLAYYGV
                     VALFMMGFVSLLTGKQLPIYRRKRLRHHLRLAILLVSGLVFGTQGALALYFPFDTLPV
                     TISHRGVDDGNAVQNSIEALEKTSQLKPDYIEMDVQETKDGQFIVMHDTDLLALTGNP
                     GGTHDYTLAELTGMTASENGMAAPVPSFDEYLEKADELGQKLLVEIKVTKADSPKLTQ
                     NFLKKYGQRLIAKGHQMQSLDYRTIIEIKQYSEKLVSFFILPFNSIYPTTVADGYTME
                     YTSLDQNFMTKSWIRGKSVYAWTPNEEESMTKMLLLQVDGIITDNMTDLQSLMEEMKE
                     NRRYPDLFFQQFQSLIYNFE"
     gene            complement(14644..15570)
                     /locus_tag="AC1PIW_000324"
     CDS             complement(14644..15570)
                     /locus_tag="AC1PIW_000324"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HIP6605292.1"
                     /translation="MRQTKEMRLVYSDFLAQVGQEDTTIAAIEADLSSSMATNKLSSV
                     LGGRYVNVGIMEQEMVGVAAGLSLLGYKPYIHTFGPFASRRVYDQVFISLAYAQLNAT
                     IVGSDAGVSAEMNGGTHMPFEELGLMRLIPKARVYEVSDDVLFQAVLKETIKVDGLKY
                     IRTIRKAPTPIYQGGEDFSKGYCVLRQGEDMTLLASGIMVEQALKAADQLEAQGVSVQ
                     VIDLFRIKPIHEDISALLSGRPVLTVENHNRIGGLGSSICELMATDLTTPVHRIGIEE
                     SFGQVGQQAYLMDVYGLTAEYIVKQAQLMLQQ"
     gene            complement(15567..16415)
                     /locus_tag="AC1PIW_000325"
     CDS             complement(15567..16415)
                     /locus_tag="AC1PIW_000325"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIP6605293.1"
                     /translation="MTSGTADLKRFAKEIRYHTLATLNQLGFGHYGGSLSIVEVLAAL
                     YGEILDIRVQNFSSKERDHFILSKGHAGPALYSTLYLKGFFDRDFLLSLNQNGTHLPS
                     HPDRNLTPGVDMTTGSLGQGMSVATGVAYGKKIEQSPYYTYTLVGDGELNEGQCWEAA
                     QFAAHHELSKMILFVDDNKKQLDGRTHDICQTFDFVEKFQAFGWESVRVNGADIDAII
                     NAILTMKSSKSPQPKCIVLDTTKGQGVTFLEELESNHHLRLSGQLREDLEQVTLVLAR
                     ELEVTE"
     gene            complement(16417..17763)
                     /locus_tag="AC1PIW_000326"
     CDS             complement(16417..17763)
                     /locus_tag="AC1PIW_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HIP6605294.1"
                     /translation="MILDFLIDIAKTPAILVALIAILGLALQKKPAADLVKGGLKTFV
                     GFIVVGGGANIIVGSLEPFGQMFEQAFNLKGVVPNNEAIVAMALDKYGTATSLIMLLG
                     MVFNIAIARFTRFKYIFLTGHHTLYMACMIAVIMSVAGFTSVGLIVMGGLALGLIMTL
                     SPAFVQKYMIQLTGNDHVALGHFSALGYWLSGVVGGLVGDKSKSTEDINFPKGLAFLR
                     DSTVSIAISMSLIYMIVALFAGGDYIKENLSGGTNSMIYALTLGGTFAAGVFVILSGV
                     RLILAEIVPAFKGISEKLVPNSKPALDCPVVYPYAPNAVLIGFISSFAGGIVSMIILA
                     LTGGVVILPGVVPHFFCGATAGVMGNASGGVRGAVCGAFMQGVLISFLPVFLLPVLGD
                     LGFAGSTFSDADFGLSGIFLGVLAKNGGILTVSVGIFAVLALMLGLSLVKKEQKEG"
     gene            complement(17817..18101)
                     /locus_tag="AC1PIW_000327"
     CDS             complement(17817..18101)
                     /locus_tag="AC1PIW_000327"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIP6605295.1"
                     /translation="MLRIGTACGSGLGSSFMVQMNIESILRDLGVSDVHVEHYDLGGA
                     NPSEADVWIVGRDLADSATHLGDVRILNSIIDMNELRELVTAICQEKGLV"
     gene            complement(18103..20145)
                     /locus_tag="AC1PIW_000328"
     CDS             complement(18103..20145)
                     /locus_tag="AC1PIW_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HIP6605296.1"
                     /translation="MVDNRTMAILMELFATRKESLYELSIQTGIEKEQLHSNLELVNQ
                     LLQEHSFPQIQYKDSEFVISTELYNQKESVFSLFRNRQIYLSQEERQLLIYLYTFIRK
                     EFVSNVHYQELLSVSRNTTLTDIKNVKELCLDFQVRLEYTRAKGYHLIGREEDKHRLA
                     LYALSNCLQSSIGVWALDYILKSWGEENPIEELKIVSQQACNYYKVSALEERLDEYLY
                     FLIFLFIRQARVGNRINWNFEGKGGFVKDFLQRLWKLLRLKSTSTLELNAATQEYLSH
                     LLQGCLEGESGEKDDLFYHLTVEIVEEMERLSLITFEHRSEMIEGLQRHLIPAYYRLT
                     SRLVNVNSYTETIKEEHADLFYLVKKALRPLEEHLGFTIPDSEVSYFVIHFGGYIEAG
                     QQRSYRYRALVVCPNGVSSSLIVKENLRQLFPNIYFADTHSLQDWKMLDMTDYDMVFG
                     TIKLNIELPFFLVSQLMTSNHKKELFHLVNQHFPNAAYFPIEIEQLLSLVGKHATIHQ
                     EQALKYELVQFLNQRSHEQRGRSPMLEELITKETFQWSEEVLDWQKAVALAARPLVDK
                     GAIETRYIDAMIAKVEEFGPFIDLGKGIAIPHARPEDGVNEVGMSMLVLDKPVYLLDD
                     PSHEIRLFICIAAVDNQTHLRALSHLTKILREEENIQQLVGTKEFADVQHLLKEEQ"
     gene            complement(20413..20907)
                     /locus_tag="AC1PIW_000329"
     CDS             complement(20413..20907)
                     /locus_tag="AC1PIW_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019783378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HIP6605297.1"
                     /translation="MGIEKTVSELAEILGVSRQAMNNRVKSLPEEFVDKNDKGVTVVN
                     RAGLIKLEEIYKTTIFEDEPISDEVKHRELMEILVDEKNAEIIRLYSQLKAKDKQLAE
                     KDEQLKVKDVQIAEKDKQLDQQQQLTLKAMADKEVLKLELDEVKAQAEEVQAKGFFAR
                     LFGK"
     gene            complement(21077..22477)
                     /locus_tag="AC1PIW_000330"
     CDS             complement(21077..22477)
                     /locus_tag="AC1PIW_000330"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HIP6605298.1"
                     /translation="MVTEQREYRFPEGFLWGSSTSGPQSEGSVAGDGKGWNNWDYWYS
                     QEPELFHQQIGPEQTSTFYEHFQSDLDLLVETCHSIFRTSIQWSRLIPEGIGEVNPQA
                     VEFYRAVFAGIRERGIKLLVNLYHFDLPYVLQEKGGWENKEIVWAYEAYARQCFSLFG
                     DLVDHWITFNEPIVPVECGYLGSYHYPCKVDAKAAVVVAYHTQLASSLAVRACHQLHP
                     DHKISIVLNLTPAYPRSQSPEDLQAARIAELFQTRSFLDPSVLGQYPRELVDILEKHG
                     LRPETTEEELTIIKENRVDFLGVNYYQPLRVQAPSEDGWNGEPTFLSQYFQPYDKPDK
                     KINPHRGWEIYEEGLYDIAKNIQENYGNIEWLVTENGMGVEGEGVFEENGMIQDDYRI
                     HFMEDHLIQLHRGIDEGANCKGYMVWTFIDCWSWLNTYKNRYGLVSLDLQTQKRTVKK
                     SGYWFKQVSAKNGFIR"
     gene            22715..23890
                     /locus_tag="AC1PIW_000331"
     CDS             22715..23890
                     /locus_tag="AC1PIW_000331"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIP6605299.1"
                     /translation="MNTVDTIIIGAGPAGMMAAISSSFYGKKTLLLEKNKRLGKKLSG
                     TGGGRCNVTNNGTLEDLLAGIPGNGRFLYSVFSQFDNHDIMNFFQENGVKLKVEDHGR
                     VFPTTDRSQTIIKCLEMKMLENGVDIRTGTEVVSVRKMDDLFHVKTSEEVFTAPQLIV
                     TTGGKTYPSTGSTGFGHDIARHFKLEVTEIEAAESPVLTDFPHKKLQGISLDDVTLTY
                     GKHVITHDLLFTHFGLSGPAALRLSSFVKGGETAFLDVLPTHSDQDLFEHLEKNREKS
                     VKNTLSELMPDRLADFFAENYDSKVKQVSQKDLTELVTLLKVLPIKITGKMSLAKSFV
                     TKGGVDLKEINPKTLESKKVPGLHFAGEVLDINAHTGGFNITYCLATGWVAGSSYSS"
     gene            24246..24944
                     /locus_tag="AC1PIW_000332"
     CDS             24246..24944
                     /locus_tag="AC1PIW_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIP6605300.1"
                     /translation="MQLKLSSISKKFDHKSILEDASFQFEQGKIYGLLGRNGAGKTTL
                     FNCIARNLTLDNGSIQFVKGEETLDYDNTDIGFTQTYPQLPAFMTAYEFVRFYMDIHK
                     DKLKSPRSPEEWLNLVGIGEEDQHRLLKDFSHGMQNKVQLLLSLIVQPPVLLLDEPLT
                     SFDPVAAHEFKQLIREAKKDSVIIFSTHILQLAQDLCDEIVLLHHQELQAVPSDKLHD
                     PDFEQEVVALLTAD"
     gene            24954..26576
                     /locus_tag="AC1PIW_000333"
     CDS             24954..26576
                     /locus_tag="AC1PIW_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605301.1"
                     /translation="MKTILRYSWYQEKYNLHRSVNGFFYYLRKLPLVGKCIPESIFKS
                     YSFKSSLFLVLHILSIPSRFLIKGFWLLFHFYHASFWMNMLVNGELTFWKILPNTWLL
                     GFMFWLLFVGLSYRLGKSLDPVISKADREFMQNFSLSQSSFLQSQLFIEPILTTIYYL
                     PASTIFCMITKEWLVFPIGLMTVLAGHLAGHALNRLLFERRIFSRRNSWHAWLWIAFV
                     FVLYVLAIVFRNQLSSGMLVPILLVQPFLIWFSYGYLKQQTNHLDYLSYCMDESLQMD
                     KKLFEMTKGNEYTRQGLQMQAKLNAKTGKDLSHLSGMTYLNALLFDRYKKVLYKKVQN
                     WVLAVVVILAALEGIRYFLDPFTFTEAYLLQFLPLSFMIMYVASSGKVVAQMVFVNCD
                     ISMLHYPFYREAKTIIAGFNYRFLQTCKLNLIFATSLFLAIMVLGRFAFSLETILLTA
                     LLLISLTALFSFHDLFIYYILQPFTNDMDVVNPLYKFLSGALYWVAYLNTRINVGSHT
                     YILLVSLAMIAYVSIGYWILLKKAPQTFRLKA"
     gene            complement(26796..27356)
                     /locus_tag="AC1PIW_000334"
     CDS             complement(26796..27356)
                     /locus_tag="AC1PIW_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIP6605302.1"
                     /translation="MTIKYPDGTIDLLSPVEVVKQADKTAPTVANDGKGNIVIVPSEK
                     AVELVVSYVDNNGKSQTVVVTKGTDGLWTASNTVVIVDPVTGQVIVPGSVIKPGTVVT
                     AYSKDEVGNSSDSAEAEVVAVDENNSAAGVKVKSVTTNANNVEKKAKQLPNTGEEANS
                     ATSLGLVALGLGLALLAAKRRRDEEA"
     gene            complement(27390..27953)
                     /locus_tag="AC1PIW_000335"
     CDS             complement(27390..27953)
                     /locus_tag="AC1PIW_000335"
                     /inference="COORDINATES: protein motif:HMM:NF019202.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HIP6605303.1"
                     /translation="MAQDVLDAAKQDAKNKIAKESDAAKSAIDANPNLTDAEKESAKK
                     AVDADAKAATDAIDASTSPVEAQSAEDKGVGAIAKDILDAAKQDAKNKIAKEAESAKS
                     VIDSNPNLTDAAKEAAKSEIDKAVEEAIVLINGVRTYQELEKIKLPMAALIKPAAKVT
                     PVVDPNNLTEKEIARIKAFLKENNNLP"
     gene            complement(27959..30490)
                     /locus_tag="AC1PIW_000336"
     CDS             complement(27959..30490)
                     /locus_tag="AC1PIW_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445096.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HIP6605304.1"
                     /translation="MSYKDMFRKEQRFSFRKFSFGLASAVIANVILGGAIANSPVVHA
                     NTVTEAETAVAPANQDLGNETKTEEEPKEPIEAVRTDMENRAAEILPEALNASVTNQA
                     PVIPTIGDLPKDASGQNVHGKATDNKIYRVVYVFGNVAGTTETEDGKQNVAPTFNRND
                     ATKTFPITDPDSDIQTISYEVPADIASYTLDDPNSIVTNGTSPGPVSYLDGPNGSATL
                     TQDGYLTGSFPWGAGDLAGRRIKVTDATGNTTKSNPFYMVAYTVKPVDDKPLAVSNSS
                     ELTEQAIFDKLVVDKSAKTTSNSALVIDSSNYKHSIAGYRTVNSDGTKTETVEETNLS
                     DFPTEGKYEVRVKTTNVYGQTIYNWIPVNAYKLDTAKDAEIRKYTDNQAPIHAIMQIG
                     QAGEKAAVILKDIPSDFSIENFNLKDGVADELAKRNLEFVRNDAVATTDTDGDGAKEG
                     IVGYIQPKTGGANSGVATYTGSNNLTYGFTYKAVETKDKANATEAKTLELDYTILFID
                     TKAPVMTPKSEYIRFVGEEYTVSVPGTDNAFLNTGKLNGTLSILKDGESGSLVSSDLG
                     TNTKITSELDPTGATANQGDDGQSSTKFNVKITGTGPATEGTGTYKLRVGEDNYPFGP
                     EGKLVDGNKPENVGLTSVKVTFVKHATVSTPVSVENPANLTPEEKAAVIAQIKKDNAD
                     NERLKGLPDSAFTVNSDGTVSVDYSAGGVNVDGATDIIKNATTNLADTRNEAKAEIDT
                     KLAEHKKAIEAKRDEAFSKIDDDISLRAEQRQAAKDAVAAAAGDALKELDNKATEAKE
                     KIDKATTASEINDAKTNGEINLDSAEAVGEKAINQSKRNRQRTKA"
BASE COUNT         8543 a         7166 c         5883 g         8967 t
ORIGIN      
        1 agggcaacac tctacagatt acttcaacag tccagtggag tgtttgaagt aggaaatagg
       61 gaaacaaagt ttctccttac gttgacctaa taaaacgact atagttccgc caagagatca
      121 gcaagccttg tcttagcaag ttcattttcc aaagctgact gagcattttc caaacggcta
      181 tctagcagag gatgaatatt gcgacctact tgacaggaag gattcggttg ctcatgaaaa
      241 gcgaataatt gacctttttc tccaaataac tccacagcct gatagacatg caaaagtgta
      301 atctggtctg gaccctgtgc caagcgagca cctccaatac cacgcgctac ttctaccaga
      361 ccagcctcct tgagttggga caaaatattt cggataatga caggattaac cccgacactg
      421 cctgcaatgc tagtactggt ctgtttttct ttttctcctt ccagagccaa gagaaccaag
      481 atatggctgg caatggtaaa tctacttgaa atttgcatct cttctccttt ttttcagtct
      541 atcatagact gaaacagaac tcaagtttga aaattagtca cggtcacacc cagcaaacga
      601 atcccttttt caatcttccc aatttcttca attagttgac gaacctcttt ctcaatcgtt
      661 tccgtctggt ccgtatatac ctctagacta tgacgtttcg tcaaggtaga aaaatcacca
      721 taacgaacct ttaaaacaat tgttcgaccc ttttttccat ttcgcttcaa actggctgcg
      781 acccgttggc agagactgat tagctctttc aggacatcct cctcccgata aagaagtttg
      841 cgataggttc tttcctttcc acttgactta cgaacacggt ccactttaac gggcgaatta
      901 gaaatccccc tcgctttccg ataaaggtcg aagccaaaac gcccaaatct atctatcaaa
      961 accatttctg gtacatctag caaatcttgc cccgtgtaga cccccatctc atgcaggcgt
     1021 tccaccgtct tcttccccac accgtgaaat ttttcaacag gcagggaggc gagaatacca
     1081 acagcatctt caggtagaat caaggtcaaa ccgtgcggtt tttccatatc agaggcaatt
     1141 tttgccaaaa atttattata ggaaacacct gcggaagctg tcagatgcaa ttcattccaa
     1201 atatcgtatt ggataagctt ggcaatctta accgcagagc tgatgcccaa cttattttct
     1261 gttacatcca agtaagcctc atcgatagac atgggctcca ccaaatcagt atagcgatgg
     1321 aaaatttctc gaacctgtcg cccaacttcc tgatattttt cataattgcc agaaatgaaa
     1381 atcgcctgag gacaacgctc ataagcctct tttgaggaca tagccgagtg aatcccaaag
     1441 gcacgcgcct cataattaca ggtagacacc actcctctac cgccagatag tcttgggtca
     1501 gccccaatga taacaggttt gcccttcaaa gcagggctat ccctgacctc aatggcagca
     1561 aaaaaagcat ccatatctac atgaataatt tttctagata aatcattgat taagggaaaa
     1621 attaacatga ctgcctcttc ctgtttacaa gtttaactcg ctatattata ccgcatttct
     1681 taggaagttt cagcctttag attcgttgaa ccagccatct taccaataca ataaacgaca
     1741 gcttatcacc ttattacaaa tacaagcaga aatttatttt gaaaatacca actctatatt
     1801 tcaatttttt tcatattcct tgaaaggttt ttcatttatt ttcaagtatt tttgtcttgt
     1861 agaaagtgtt ttcaaatggt ataataatga catgttggta tcgatagcaa gtcgataccc
     1921 aaaagcataa aaggagaacc ctcatgtcaa caaaagttaa aacaaaaaat gttgctgaag
     1981 acattttcgc ccaagcctgg gaaggcttta aaggtacaga ttggcaagat aaggcaagtg
     2041 taacacactt cgttcaagcc aactacactc catacgatgg agatgaaagc ttcctcgcag
     2101 gcccaactga gcgttcactt catatcaaaa aaatcatcga agaaacaaaa gctggctacg
     2161 aagacactcg cttcccaatg gacgtagatc gtgctacttc tatcgctgat attccagcag
     2221 gtttcatcga taaagagaac gaactcatct tcggtatcca aaacgatgag ctcttcaaac
     2281 tcaatttcat gccacgtggt ggtattcgta tggctgaaac aactcttatc gaaaacggct
     2341 atactccaga cccactcctt catgaaatct atacaaaaca cgctacaact gtaaacgacg
     2401 gtatcttccg tgcctacacc gctgacatcc gtcgtgctcg ccactcacac cacgtttctg
     2461 gtcttccaga tgcatactca cgtggtcgta tcatcggtat gtacgctcgt ttggcacttt
     2521 acggtgcaga ctacttgatg gaagaaaaag tagctgactg gaatgccatt actgaaatcg
     2581 atgaagaatc aatccgtctt cgtgaagaaa tcaacatgca gtaccaagca ttgcaacaag
     2641 ttgtacgctt aggtgatcac tatggtgttg atgttcgcaa acctgcaatg aacactaaag
     2701 aagctatcca atggacaaac attgctttca tggctgtatg tcgtgttatc aacggtgcag
     2761 caacttctct tggtcgtgtg ccaatcgtac ttgacatcta cgctgaacgt gatttggctc
     2821 gtggtacatt tactgaggct gaaatccaag aatttgttga tgacttcgta ttgaaacttc
     2881 gtacagtgaa attcgctcgt acaaaagcct tcgacgaaat ctactctggt gacccaacat
     2941 tcttgacaac atctatggca ggtatgggta acgatggtcg tcaccgtgtt actaagatgg
     3001 actaccgttt cttgaacaca cttgacaaca tcggtaactc tccagaacca aacttgacag
     3061 ttctttggtc agaccaactt ccatattcat tccgtcgcta ctgtatggca atgagccaca
     3121 aacactcttc tatccaatat gaaggtgtga caacaatggc gaaagatggt tatggtgaaa
     3181 tgtcatgtat ctcttgctgt gtatctccac ttgatccaga aagcgaagac caacgccaca
     3241 acatccaata ctttggtgct cgcgtaaacg tccttaaagc ccttctttca agctggaaca
     3301 acggttacga tgacgttcac aaagattaca aagtattcga cggtgttgaa ccaaacacat
     3361 ctgaagtatt tgactacgac caagtggtta agaactttga aatcgccctt gactggttga
     3421 ctgatacata tgttgatgcc atgaatatca tccactacat gacagataaa tacaactacg
     3481 aagcagttca aatggcattc ttgccaactc accttcgtgc taacatgggc ttcggtatct
     3541 gtggtttcgc aaacactgtt gactcattgt cagcaatcaa gtatgcacaa gttaaaccaa
     3601 tccgtgatga agatggattt atctacgact acgaagtaac tggtgacttc ccacgctatg
     3661 gtgaagacga tgaccgtgta gatgatattg cgaaatggct catggaagca ttcttcactc
     3721 gtttgaacaa acataaattg tacaagaatg ctgaagctac agtatctatc ttgacaatca
     3781 cttctaacgt tgcttactct aaacaaacag gtaactctcc agttcaccgt ggtgtattcc
     3841 tcaatgaaga tggttctgtg aacactacta aggtagaatt cttcccacca ggtgctaacc
     3901 caacttctaa agctcgtggt ggttggttgc aaaacttgaa taccctttct aaactcaact
     3961 tcaaacatgc caacgacggt atctcattga ctactcaagt ttctccaaaa gcgcttggta
     4021 aaacattcga tgagcaagta aacaacttgg taacaatcct tgatggttac tttgagcaag
     4081 gtggtcaaca cgttaacttg aacgttatgg acttgaacga cgtttatgaa aaaatcatgg
     4141 ctggtgaaga tgttatcgtt cgtatctctg gatactgcgt aaacactaaa tacctcacta
     4201 aagagcaaaa aactgaattg actcaacgtg tcttccacga agttctttca atggacgacc
     4261 acgctgctga agtttcaggt caagcttaat caagcagaca caaaaggact tgcacatgca
     4321 ggtcctttta ctatgtcgaa aaatctactg agtgttgcgg aaggatagtt atagtctttt
     4381 agtttacttt tagtactagg caacgagctg cggacagctt gtacagtcga gtgactgtgc
     4441 aaggttggag atagcacttg cggagcaagt cccccctgca gagtacggca aagcgagttc
     4501 aaatactcca gtggagtgtt tgaagttgga aataaggaaa cgatgtttcc tcgatcgtcg
     4561 aagtaataaa agaaaaacta aatgactgat atatactcaa tgaaaatcaa aagtagccta
     4621 agaaacgaag tcgaagatag aactggagtt cattaaggca agttgacaat ggataatttt
     4681 gattttcgaa gagtattagt caatagcctc ttcttcctcc aagacctgac gaatcaaggc
     4741 cataacctgg tttctatcca acttccactg ggcgcgctca tccttgtcaa aagtacgatt
     4801 cgagagaaaa atcgcagctt tctgcaaggg acggttatac atgataaagg tccccgtata
     4861 gcctgtgtgc gacaaccagt cccccttctt atcccaagcc agagaccgct tgcgtttatc
     4921 atcaaaaccg taatcctgcg ttaaattcgc cgcaaaatcc tcagttagat agcgctccaa
     4981 aaagatttcc aaatctttga cggtcgaaaa gagacctgca cttcccgcgt ggaggcccaa
     5041 gacacgcgcc ttgggatcat gcaccacacc tgctttgacg ccacgaaccg tcggcaccgc
     5101 ctgactgact ggtccaaagg aggttcggaa cataccaaag ggctctagaa cgtctcgact
     5161 aatcagctgg tctagactgt ctctacccaa ttcctccaac aaaaagccca gtaagagaaa
     5221 attaatatcc gtgtagagga aagctttctt atccgttaca gcaatcttca aaatcgcttc
     5281 cttgagctgg tcggcatcca gactatccct atttggaata aagggatcaa taccactagt
     5341 atgggtcaat aattgccgaa tggtgacctc actatccgca aaagctggat aataagactg
     5401 caaggtccta tctagctcca aagctccgct attaaccaaa aaagtcgcaa gagtccctac
     5461 tccaaccacc ttagaaacag acgccaaatc ataggtcaaa cccgcctcaa ctggcgtatg
     5521 cccatcctgg gtccctagat agtactcctg ccactgacca tcagaaaaaa agccagactg
     5581 acaccaggat atactccttg ctcaatctgc tctgaaacct tgtctaaaat tacttgtctc
     5641 atttttcgaa ccacaattca ataccacttg ggtcaactgt ttggataaag ctcgcttttt
     5701 tatctttaaa gaatggtctt tccaaccggc tttccaaatg cgcccaatca aagtcttgct
     5761 cgacgggaaa acgcaagcct tctaaatccc aaacttgttc tgcaggagtt aataaatcct
     5821 taccttctgc tccaacaaaa cgcaaaaagg cttgttgagg taggatagcc tcataatagt
     5881 cttgactaat actagcttgt ggggtattaa tccagacaga ctctactgta aaagcggtca
     5941 gaccagaaaa gtcatcctgc accttaaatg gaacaggtgg aaggattgct tttaggtcat
     6001 caacagatgc ttctgcgtgt aaaagaaagg tgtctccttc tggcgaaaga gcttcaaaac
     6061 catagccatt ttttccttgg tagagcttac tatactgtga cccttgcgcc agtaaagctt
     6121 caatttcaga ggcattgtct acttttatca caattttatt cagtttttta agcccttcga
     6181 cagcacgggt gcgattacca ggagattctg tcaaaacgag cttcgcttct ggacttgttc
     6241 tatctccaaa atcagcaaaa ggaccatctt cgagcttggt tttcattcct agattttctt
     6301 ctaaaaatcc ttggttaata gccctattat taatacgtaa aatagggact atagcttcat
     6361 tttttctcaa cacgatacat ttcactcctc agtcattcta caatattgta taaaaatttt
     6421 catcatttta caagtaattg tatcgtcgtt ttatacatta atatctctat atatactgat
     6481 aatttttaga caaacattga cttatccgat ataatagcta ttatagacta ttatcctaaa
     6541 aaagaggaga atttatgcgc tttaatcaat tttctttcat aaaaaaagag acctccgtct
     6601 acttgcaaga acttgatact ctgggatttc aactgattcc tgatgcaagt agtaagacaa
     6661 atttagaaac ctttgtcaga aagtgccatt tcctgacagc aaacaccgac tttgccctgt
     6721 caaacatgat cgcagaatgg gacacagatc ttctgacctt cttccagtct gaccgtgagt
     6781 tgactgacca gattttctat caagtcgcct ttcagttgct gggctttgtg cctggtatgg
     6841 attatacaga tgtcatggat ttcgtggaga aaagcaactt cccgattgtc tatggggaca
     6901 tcattgacaa cctctaccag ctcctcaata ctcgcaccaa gtctggaaac accttgattg
     6961 accagctagt cagcgatgac ctgattccag aggacaatca ctaccacttc ttcaacggca
     7021 aatctatggc aaccttttca accaaaaacc ttattcgtga ggtggtctat gtcgaaacgc
     7081 ctgttgatac agctgggact gggcagacag atattgttaa gctgtccatc ctccgtcctc
     7141 actttgacgg gaaaatccct gcggtgatta caaatagtcc ttatcatcaa ggagttaacg
     7201 atgtagccag cgataaggct ctgcataaga tggaagggga actggctgaa aaacaagtcg
     7261 gtaccattca ggtcaagcag gccagcatca ccaagttaga cttggaccaa cggaacctgc
     7321 ctgtcagccc tgctactgaa aaactggggc acattacttc gtactccctc aatgactact
     7381 tcctcgctcg cggctttgcc agcctccatg tgtctggtgt tggcacgctg ggctcgacgg
     7441 gctacatgac atctggcgac taccaacagg tggagggcta taaagcggtg attgactggc
     7501 tgaacggtcg caccaaggcc tacacagacc acactcgctc gcttgaggtc aaggccgatt
     7561 ggcccaatgg taaggtagcg acgacgggac tgtcttatct cggtaccatg tccaatgcct
     7621 tggcaacaac tggcgtggac ggattggaag tcatcatcgc agaagcaggg atttcctctt
     7681 ggtatgacta ctaccgtgaa aacgggctgg tgaccagccc tggtggctat ccaggcgaag
     7741 atttggacag cttgaccgcc ctgacctact ccaagagttt gcaagcaggt gacttcctcc
     7801 gcaataaagc agcctacgaa aaaggactgg ctgccgagcg tgctgccctg gaccgcacca
     7861 gcggtgacta caatcaatac tggcatgacc gcaattatct gctccacgct gatagggtca
     7921 agtgtgaggt ggtctttacc cacggctcac aggactggaa tgtcaagcca atccatgtct
     7981 ggaatatgtt ccatgcccta cctagtcaca tcaagaagca cctcttcttc cacaacggtg
     8041 cccacgttta tatgaacaac tggcagtcta tcgacttccg cgagtccatg aacgccctgc
     8101 tcagtcagaa actactgggc tacgagaaca actaccaact gccaacggtt atctggcagg
     8161 acaacagcgg tgagcaaact tggacgacct tggacacctt tggcggtgaa aacgagactg
     8221 tcttgccact aggtactgga agccagaccg ttgccaatca gtatacccaa gaagattttg
     8281 aacgctacgg caaatcctac tctgccttcc accaagacct ctatgcaggc aaagccaatc
     8341 aaatcagcat tgaactgcct gtaacggagg gcctcttgct taacgggcag gtcacgctga
     8401 aactccgtgt cgcttctagt gtagctaaag gcctcttatc cgctcagcta ttggacaagg
     8461 gaaataaaaa acgcctagcc ccaatccctg cgcctaaagc aagattgagt ctagacaacg
     8521 gtcgttacca tgcccaagaa aatctggtgg agttgcccta tgtggagatg ccacaacgct
     8581 tggtaaccaa gggctttatg aacctgcaaa accgcaccga cctgatgact gtcgaggaag
     8641 tggtgcctgg acagtggatg aatctgacct ggaaactgca gccgaccatt tatcagctga
     8701 aaaaaggcga tgtcttggag ttgattctct atacaaccga ctttgagtgt actgttcggg
     8761 ataattctca atggcaaatc caccttgatt taagtcaatc acagttaatt ttaccgcact
     8821 aatactcaat gaaaatcaaa atcagactag tttcaaaggt tttggaaacc tttgaaggtt
     8881 ggagatagag tgaacgaagt tcgtttctcc aagagcagat atacccctgc tactttctta
     8941 ttttatggta gcaggctgtc aagctccact ggagcttgac actcatcaaa tcaagtcaac
     9001 aacgtctgat tttgagtttc gaagagtata atattgattt tcagttctaa aaagaggctg
     9061 ggcgtttccg ccctgcctct ttattatttc ataatggttt agatttgatc tttagatttt
     9121 ttgtttatag ctgtagcgac taccgccaag ctaattccag ctaaaccaag gcctactaag
     9181 gctaggttat tgcgctgttc acctgttgca ggtagggttg ctttgtttgc agaaggtgcg
     9241 cccttatgac ttagtgtcgg atttgattgt actgaaggag ttgtttttac ttcttctttc
     9301 ttcatttcca agctaccttt tacaaggata cgattttgac cttcaacaac agtttctgat
     9361 aataggttgg ttgcgacaac tttaccatca actatgacat cttgataaac agatactttt
     9421 ttacctgctt ttccttcttg aagaaggtat gatttgccaa gcggtaggct tgcatcatat
     9481 ctgatttctt ctttgatagg gagagcttct tctaccttgc gttcttttgt tgtgaagtct
     9541 agctttggtt tttccgctgt ttttggtgcg acttctggaa ctgctgtctt aggtgcagag
     9601 tcttccgctt ttggttgctc agactttggc atttcctcct tcggcatgtc ttctgctttc
     9661 ggttgctcca tttctggttg cttagcctct ggcatctgtt tgtcagactc tactttatca
     9721 gttttcggtt tgtccattgg agtttctggc attggagttg gggctggaac ttcttcctct
     9781 gctttcggcg ctggagcatc tggcattggt gccggaactt cgtcctcttt cggtgccgga
     9841 gtgtccggta ctggagttgg agtttcttct ttcgtctctg gtgccttctc ttctgttttc
     9901 ggctcctgtg tttcctcttc tactttcggt gttggagcgt ctggaattgg tgccggaact
     9961 tcgtcctctt tcggtgccgg agtgtccggt actggagttg gagcctcttc ctctgccttt
    10021 ggagtgtctg gaattggagt tggagctgga tccacttccg gtgttggagc tggagctggg
    10081 gctgcgtccg gtgccggagt gtcctcctct tttggagctg gggtttcttc ctcttttgga
    10141 gctggagctg gctcatctgc gggacttggc tggggttgtt ttgatttttc cagttcagca
    10201 actcgttgct taattctgcc catggcctca cttgcttctc tatcaacgta gtctcttaag
    10261 gtagcgccct cttggctagc tatactcttg ccttcgtctt gctttaaatg cgcattatat
    10321 tcttcctcaa ttgtattatc accagggtat ttctttcgca attctgcaac ctgacggata
    10381 tagttttcca cttttatttg atatttaaaa tcattcaaat cccctttaac cttatggaaa
    10441 aacttgtcat ctgctttcat ttttgaagca tagtccatat tatcttttcc aattcttaaa
    10501 aactcttccc acaaagcctt ctgataagaa taatcttgag catcaccata acttggaacc
    10561 ttatctagtt cccccagctc tttttctaat tctttcacaa gcttctgaac ttcttcaact
    10621 ttcgccattg cttcaacttg agtagctgtt gaagtctctg tcgtcgcaaa agcggttggt
    10681 gtcgagactc ccgcaccaag taagcaagta gcaatcagca ccgagcctac gccgacttta
    10741 aatttacgaa tactatatct gaatttttta tttgcagttt ccatttctat aagtcctaat
    10801 catattttat tatatattat tattgtacca tgatattcag gtggtcggat gtgtttttta
    10861 tgtgttaaaa ctctaccgaa tagctctgtt tcttcatcgt tgaatgcatc tgcctatttt
    10921 tctaaaggat ggatgataac accttgcacc acacggttga cggtgcctgt tggagatggt
    10981 gtatctggca agttgttgca cttaatggct gccataatag ataccgtttg ggcaaagagg
    11041 atgtaaggga aggttaggaa gacatctggc agatctgctc cgccttctgc caagacaaag
    11101 ttggctgctt ctgtaccgac taacttatca gccgacaatg ccactatccg ctctgctatg
    11161 ccatcaccat gaacttcatt aagcaagtcc acatcatact gacgagtgta ggcatcgttt
    11221 gaggcaaaga ggaaaatcaa ggtcttttca ttgatgaagg attttggtcc atgacggaaa
    11281 ccgagtggtg actcgtacat ggtcgcaatc ttacctgctg tcaattccag aattttcaac
    11341 tgagcttcgt gggcaagacc atagaaacca cctgaaccga ggtaaatcac tcgctcaaaa
    11401 tcaaggttaa tcaagtcttg cacgtagtct tcacggtcca aaacatcttg acctagacgg
    11461 gcaatgatct caacccaagc agctttttct tcttgactgg ctgacgagaa gacgaggagg
    11521 gctgtcagag ccatacagct gtaagaacct gtcatggcaa agcccttatc gtttgatcca
    11581 gccggttgca agagcaggag attttcagca tctccttcag ccgctagtgc caacttacct
    11641 tcgggtgcac aggtaatggt cacttgatac aatttatcta ccaattgctt agcgacttca
    11701 actgctgcca cactttctgg tgaattccct gaacgggcaa aggataccaa aaccgttggc
    11761 acatctgcct gtaaatagtc atgtgggcga ctaaccagct ccactgtacc aatggcagaa
    11821 aaacgaatgt gtttcaagtc attgacttgg ttgaggtagt tggcaatact ttgcccaaca
    11881 aagtcagaag tccctgcacc tgtaaaaaca acgtgaacaa agtcatgttt ttccttgatt
    11941 gattttagaa aactctcgat tttttccaat tggtcaaaat agagttgata cgcttccttc
    12001 cataagcctg gctgttggta aatttcggtc gccgtaataa tagctcccaa cttttcaagt
    12061 tcttcttttg ctaaacggaa catagctcct cctccgtgat ttctggttat aaccagtttt
    12121 tcttatatta tagcgaattt atccctcaaa gtcaatttaa aatagattaa tatgtcaaat
    12181 ttatatccga tattgctgac tataacttat tttaagaagg tagcattatc tacataaaac
    12241 atgttataat atttactacc cctctgcctt tgaaaaagtt taacataagg caaggaaaat
    12301 ttacaaacag ggtctaaccc cttgatacga aaggaatctt aaaaatctta tgatgaacat
    12361 gcaaaacatg atgcgccaag ctcaaaaact tcaaaaacaa atggagaaga gtcaagctga
    12421 gttggctgct actcaattta ctggtagctc tgtacaagac ttggttactg ctacttttac
    12481 tggagataag aaattggttt ctatcgactt caaggctgat gttgtggatg cagacgacct
    12541 tgaaaccttg caagaaatga caatccaagc cgtcaacgct gcacttacaa aagttgacga
    12601 ggcaactcag aaaaaactag gtgcctttgc aggtaaatta cctttttaag acagaaaaga
    12661 cgcgattggg ccaggccctc gcgtctcttt gtttattcaa aattataaat caaagactga
    12721 aattgctgga aaaagaggtc tggatagcgt cgattctctt tcatttcctc catcagactt
    12781 tggaggtcgg tcatgttgtc agtgatgata ccgtccactt gtagcagcaa catcttggtc
    12841 atactctctt cttcattcgg tgtccaagca taaaccgact tccctcgaat ccaagacttg
    12901 gtcataaaat tttgatccag cgaggtatac tccatagtat agccgtcagc aaccgtcgtc
    12961 ggataaattg aattgaacgg aagaataaag aaggatacta atttctcgct atactgcttt
    13021 atttcgataa tagtacggta atctaatgat tgcatctggt gtcctttggc aatcaaacgt
    13081 tgaccgtatt tttttaagaa attctgggtg agttttggcg aatctgcctt ggtcacttta
    13141 atttctacca ataatttctg tcccagctca tccgcttttt caagatattc atcaaaagaa
    13201 ggaactggag ctgccatgcc gttttctgat gcagtcatcc ctgtcaactc tgcaagagta
    13261 tagtcatgtg tacctccggg attgcccgtc aaagccaata aatccgtatc atgcatgaca
    13321 ataaactgac catccttcgt ctcctgaaca tccatctcaa tataatctgg cttgagctga
    13381 gaggttttct ctagggcctc gatagagttc tggacagcat tgccgtcatc gacacctcgg
    13441 tgagaaatgg taaccggcag agtgtcaaag ggaaagtaga gggcaagcgc tccttgggta
    13501 ccgaacacca gaccagatac gagcaaaatg gctagtcgaa ggtgatgacg gaggcgttta
    13561 cggcggtaga ttggtagttg ttttcctgtt aggaggctga caaatcccat catgaagagt
    13621 gcaactactc cgtagtaggc taattttaga agtgcgtaac agacaactgc cagccagtaa
    13681 gaagcacttg gtacaaaatg ctctgctaac agttgcacaa gatagaatag tcctcctact
    13741 cctgtaaata ggagaactgg aaaagtaacc aaccaaatta gacggaacaa tgaaccgatc
    13801 tgctttctgc ctctggtctt ctcccaagaa taagcgatag actccttcac acccctgtgt
    13861 tcaaaataaa tgtttggcag agcgtacatg aagcgtgccg caagccagaa gaacaagagg
    13921 aggaaaatca aaatcaatat tcctagccag actgtgttgg ataggtaatc tacgataaat
    13981 tggggcacta cgattttatt aaaataatag atatttaata ctcggcgtaa aaatggaaga
    14041 agaataactg agtaaaagag cagaaataga acctttggta ggctactgtg acggataatc
    14101 gccaccatac tatctcgtac atcccgtaaa tagtcggtga ggtgcttgac tttttcatcc
    14161 aacagttgcc aaacccctgt gaacaataga gctaattcta gataggcaat ggcaatgttg
    14221 acaaaaaaga ggagaataaa caagcctgtc acgagcagat tttcagcaaa caccgacaac
    14281 aaattcgtcg gtgaaaggta ggcatgccct gtcatagcta agagtccctc agaaatccat
    14341 gaattgagcg gtatccaaac aaattccata aaggtaaaca gggtaaagaa gagcaagaga
    14401 atcttgtcta agttgtaata tattttttga aattcacgtt gtatcttttt catgattcta
    14461 gtgtcccata ttctagcaag aattgcaagc cttcatcaag gatggaagat tgtgaaagca
    14521 atgaatgaag tatagaaaaa atcaagttcc taagtggagc ttgatttctt acatttgatt
    14581 tgctttccta tttccaagcc aaagtaaatc gaaaggctac ataaaatacg aatcactatc
    14641 tcactattgt tgtaacatta attgtgcttg tttaacaata tattctgctg tcaaaccata
    14701 tacatccatt aagtaagctt gttgcccaac ttgaccaaaa gattcctcaa taccaattcg
    14761 atgtactggg gtcgttaaat ctgtagccat taattcacaa atgctactac ctaggccacc
    14821 aattcgatta tgattttcta cagttaatac tggccttcct gataatagag ctgatatgtc
    14881 ttcatgaatt ggtttgatac gaaaaagatc aatgacttga acagatactc cttgagcctc
    14941 taactgatcc gccgccttta atgcctgctc taccataata ccactcgcaa gcaaggtcat
    15001 atcctcccct tgcctaagga cacagtagcc tttgctaaaa tcttctccac cttgataaat
    15061 aggcgttggc gctttccgaa tggtacgaat gtacttcaac ccgtctacct tgatggtttc
    15121 tttaagaaca gcttgaaaca ggacatcgtc actgacctca taaacacgcg cttttggaat
    15181 gagccgcatt aagcccagct cctcaaacgg catgtgagtc ccaccattca tctccgctga
    15241 tacccccgca tccgacccta caatcgtggc attgagctgg gcataagcta gagagataaa
    15301 cacctgatca tagactctac gactagcaaa tggtccgaag gtatgaatgt atggcttgta
    15361 tcctagaaga gataatccag ctgctacccc taccatttcc tgctccatga tacctacatt
    15421 gacatagcgt ccccccagaa cagaggacaa tttgttggta gccattgaac tggataaatc
    15481 tgcttcaata gctgctatgg ttgtgtcttc ctgccctacc tgagctagaa aatcactata
    15541 aaccagtcgc atctcctttg tctgtctcat tccgtcacct ccaactctct cgctaaaaca
    15601 agtgttactt gctctaaatc ttcccgtaat tgacccgaca aacgcaaatg gtgattgctt
    15661 tctaattctt ctaagaaggt aactccctgc cccttagtag tatcaagaac gatacatttt
    15721 ggttgaggag acttacttga cttcattgtt agaatagcat ttataatagc atctatatcg
    15781 gcaccattca cacgcactga ttcccaacca aaagcctgaa atttttcaac aaaatcaaag
    15841 gtttggcaaa tatcatgggt acgaccgtct agttgttttt tattgtcatc cacaaacaga
    15901 atcattttcg atagttcgtg gtgggctgca aactgagctg cttcccagca ttgaccttca
    15961 ttcaattccc cgtcccctac cagagtatag gtatagtacg gtgactgttc tatcttctta
    16021 ccataagcaa cacctgtcgc gacactcatc ccttgcccca acgaacccgt tgtcatatcc
    16081 acccctggcg ttaagtttcg gtcagggtgt gaaggcaaat gtgttccatt ttgatttagc
    16141 gacaagagaa aatctctatc aaagaagcct ttcaaataca acgtactata aagtgctgga
    16201 cctgcatgtc ccttagacag aataaagtga tcccgctcct tagaagagaa attctggaca
    16261 cgaatgtcca gaatttctcc atatagagca gccaacactt ctacgattga taagctacca
    16321 ccataatgac cgaatcctaa ttgattcaaa gtcgccaagg tatggtagcg aatttctttg
    16381 gcaaatcgtt tcaaatcagc tgtaccacta gtcatattat ccttctttct gttctttttt
    16441 caccaatgac aatccaagca tcaaagccaa gacagcaaag ataccaaccg aaactgtgag
    16501 gataccgcca ttcttagcta gcactcccag gaaaatacca gaaagaccaa agtctgcatc
    16561 tgagaaagtt gaaccagcaa aaccaaggtc tcccaaaaca ggaagtaaga atacaggtaa
    16621 gaaactaatg agaacccctt gcataaaagc accacaaact gcgccacgaa caccacctga
    16681 cgcatttccc atgacccctg cagttgctcc acagaagaag tgtggtacga cacctggaag
    16741 gataacgaca ccgccagtca aggcaagaat aatcatgctg acaataccac ctgcaaaact
    16801 agagataaag ccaatcaaca cggcgtttgg tgcataagga taaacaactg gacagtctag
    16861 ggctggtttg gaattcggaa ccaacttctc tgaaattcct ttgaaggctg gaacaatttc
    16921 cgctaaaatt aagcgtacac cagataagat aacaaaaaca cctgctgcaa aagttccacc
    16981 tagggtcaag gcgtaaatca tgctgttagt accaccacta aggttttcct taatataatc
    17041 accgcctgca aacaaagcca caatcatgta aatcaaggac atagaaatgg caatgctgac
    17101 agttgaatca cgcaagaatg ccaaaccttt agggaaatta atatcctcag tagacttaga
    17161 cttatcaccg accaaaccgc ctactacacc actcaaccag taaccaagtg cactgaagtg
    17221 acctagggcg acatgatcat ttcctgtcaa ttgaatcatg tatttttgaa cgaaggctgg
    17281 ggagagtgtc ataatcagac ctaaagccaa cccacccatc acaattaggc cgacagaagt
    17341 gaatcctgca acactcatga taactgcaat catacaggcc atatatagtg tgtggtgtcc
    17401 tgtcaagaaa atatatttaa agcgtgtaaa gcgggcaata gcaatattga agaccattcc
    17461 aagcaacata atcaaggatg tagcagttcc atacttatcc aaagccatcg ctacaatagc
    17521 ttcattattt ggaacaaccc ctttcaagtt gaaagcttgt tcaaacattt gaccaaatgg
    17581 ctcaagcgag ccaacaataa tattcgctcc gccaccaaca acaataaaac caacaaaagt
    17641 tttcaagccc cccttaacta aatctgctgc aggctttttc tgtaaggcta aacctagaat
    17701 agcaatgaga gcaaccagaa ttgccggagt tttagcaata tcaatgagaa aatccaatat
    17761 cattttctgt ctcctttata attttatctt tgcgggtcat cccctgtgta tacgatttaa
    17821 accaaacctt tttcttggca gatagccgtg acaagttccc tcaactcatt catatcaata
    17881 atactattca atattcgaac atctcctaaa tgagttgcag aatcagccag gtcacgtccg
    17941 acaatccaca catctgcttc actcggattg gcaccaccta gatcataatg ctccacatga
    18001 acatctgata cacctaaatc tcgcaaaatg gattcaatat tcatttgaac cataaagcta
    18061 gagcctaaac ctgaaccaca ggctgttcca attcgtaaca tattactgtt cctcctttaa
    18121 taaatgttgt acgtcggcga attctttggt gccgacaagt tgttgaatat tttcttcttc
    18181 tcgcaaaatt tttgtcaaat gcgagagtgc tctgagatgg gtttgattat ccacggctgc
    18241 aatacaaatg aacagccgaa tttcatgact agggtcatcc aatagataaa ctggcttatc
    18301 caataccaac attgacatcc ctacctcatt aaccccatct tctggtcgtg catgtggaat
    18361 cgcaattcct ttacctaggt caataaaagg accaaactct tctaccttcg ctatcatagc
    18421 atcaatatat cgagtttcta tagctccttt atctaccaga ggtcgtgcag ccagggcaac
    18481 tgctttttgc cagtctaaaa cttcctcaga ccattgaaag gtctcttttg taatcaattc
    18541 ttctaacata ggactccttc ccctttgctc atgggagcgt tgattgagaa actgcaccaa
    18601 ttcatatttt aaagcctgct cctgatgaat ggttgcatgt ttcccaacaa gtgataataa
    18661 ctgctcaatc tcaatcggaa aataagccgc atttgggaaa tgctgattaa ccaaatgaaa
    18721 taattctttt ttatgattag aggtcatcag ctgggatacc aagaaaaatg gtagctctat
    18781 attcagcttg atagttccaa ataccatgtc atagtctgtc atgtctagca tcttccaatc
    18841 ctgtaaggaa tgggtatcgg caaaatagat attaggaaat agttgccgta gattttcttt
    18901 tacaatcagc gaagagctca ctccatttgg gcaaacaacc aaggcccgat aacgatatga
    18961 acgttgttga cctgcttcga tgtagccacc aaagtggatg acaaaataag atacctcact
    19021 atctggtata gtaaaaccta gatgctcttc caaaggtcgc aaagcttttt ttacaagata
    19081 gaacaagtcg gcatgctctt ctttgatggt ttcagtatat gaattgacat ttaccaaacg
    19141 agatgttaaa cggtagtaag caggaattag gtgacgttgc aaaccttcaa tcatttccga
    19201 gcgatgttca aaagtaatta aggacaaacg ttccatctct tccactattt ctacagtcaa
    19261 gtggtaaaat agatcatcct tttctccgct ctcaccttct aagcatcctt gcagtaaatg
    19321 tgacagatac tcttgtgtcg cagcatttaa ttctaatgtg ctagtacttt ttaaacgtaa
    19381 taatttccaa aggcgttgca gaaaatcttt tacaaaccct ccctttcctt caaaattcca
    19441 gttaatacgg ttgccaacac gcgcctgacg gataaataaa aatatcaaaa aatacaaata
    19501 ttcatccaat ctctcttcta aagctgagac tttataataa ttgcaagctt gttgactcac
    19561 tattttcaat tcttcaatag gattttcctc accccaagat ttaagaatat aatccaaagc
    19621 ccacacacca atcgaagact gcaaacaatt actgagtgca taaagcgcca agcgatgttt
    19681 atcttcttct cgaccaatta gatgatagcc tttcgccctc gtatattcca atcgtacttg
    19741 aaagtccaag caaagttcct ttacattctt aatatccgtc aatgttgtgt tacggctcac
    19801 actcaataat tcttggtagt gaacgtttga tacaaattct tttcggataa aggtataaag
    19861 atagataaga agctgtcttt cttcttgcga aagatagatc tggcgattgc gaaagagcga
    19921 aaatacactt tctttttgat tgtaaagctc cgtagaaata acaaactcgc tatctttgta
    19981 ttgtatctgc gggaaggaat gttcttgcaa caattgattg actagttcca aattagaatg
    20041 tagctgctcc ttttcaattc ctgtttgaat actaagttcg tacaaacttt cttttcgagt
    20101 cgcaaacaat tccatcagaa tcgccatggt acgattatca accatatttt cctccttgtc
    20161 ttaattgtat cccttttcat ttctgatttc tagagtcttt gagcccaatt aatataatga
    20221 aaaataaaaa ttcaattgtg ctcactactt aaactaaaaa agattggttc ccaccaatct
    20281 ttttatatca catatcattt tccagttttt agctcgggct gaaacagtct atccccaggc
    20341 tgtttcactc ccccggacct ttattcgctc tcttgttttc aagctcatga taaaaaggtc
    20401 ccccggacct ttttatttcc caaataaacg tgcaaagaag cctttagctt gcacttcttc
    20461 tgcctgtgct ttaacttcgt ccaattccaa cttgagaact tccttatcgg ccatagcttt
    20521 tagtgtcaat tgctgttgct gatccaactg tttatccttc tcagcaatct gcacatcttt
    20581 aaccttgagt tgttcatctt tttcggcaag ttgcttatct ttagctttta attgactata
    20641 taagcgaatg atttcagcat ttttctcgtc aactaaaatt tccatcaatt cacgatgctt
    20701 gacttcatca ctaatcggct catcttcaaa aatggttgtt ttgtagattt cctccaactt
    20761 aatcaagcca gcacgattta caacggtcac acccttgtca tttttatcta caaattcttc
    20821 aggaagtgat ttgacacgat tattcatagc ctgccggctc actcctaaaa tttcagctaa
    20881 ctcactgact gttttttcga ttcccataat atcctcggta cttacatttc tcatagtttg
    20941 tatcattaaa atattaccac agaaccctat ccctgtcaaa tctaggggaa tttccttacc
    21001 aagcattagt gaataatcac aaacctcaaa agcatcttat ctcaatgcaa tgttatgcag
    21061 aaacgaaata tacattctat ctaataaaac catttttagc actcacttgc ttgaaccaat
    21121 atccagattt tttgacagta cgcttctgag tttgaaggtc aagcgagacc aaaccgtatc
    21181 ggttcttata ggtatttaac caagaccagc agtcaataaa ggtccagacc atatagccct
    21241 tacagttcgc cccttcatca atacctcgat ggagctgaat caaatggtct tccatgaagt
    21301 gaatccgata gtcatcctga atcatgccat tttcttcaaa aacaccctcc ccttcgactc
    21361 ccatgccgtt ctcagttacc aaccattcga tatttccata gttttcctgg atatttttag
    21421 caatatcgta cagcccttct tcgtaaattt cccagcctct atggggattg attttcttgt
    21481 caggcttatc ataaggctgg aaatattggg ataggaaagt aggctctccg ttccatccat
    21541 cttctgaagg agcctgcacg cgcaggggct ggtaataatt cacccccagg aagtcaaccc
    21601 tattttcttt gataatggtc aattcctcct ctgtggtttc tggacgtaaa ccgtgttttt
    21661 caagaatgtc aaccagctcc ctcgggtatt gtcccaaaac ggatggatcc aaaaagcttc
    21721 tcgtttgaaa tagctcagca atccgtgctg cctgtaaatc ttctggactc tgacttcttg
    21781 gataggcagg agtaagattg agaacaatgg aaattttgtg gtctgggtgt agttggtgac
    21841 aggcccgaac tgccaaagaa ctggctaatt gcgtatgata cgctacgacc acagctgcct
    21901 ttgcatccac cttgcaagga taatgatagc ttcctagata gccacattct actggaacaa
    21961 tgggttcatt aaaggtaatc caatgatcca ccaaatcccc aaagaggctg aaacactggc
    22021 gagcataagc ctcataggcc cagacaatct ccttattttc ccagccccct ttttcctgta
    22081 agacataagg caaatcgaaa tgataaagat tgaccaaaag ctttatccca cgctctcgaa
    22141 tacctgcaaa tacagcccta taaaactcca cagcttgcgg attgacctcg cctatccctt
    22201 ccggaatcag acgtgaccac tgtatagagg tgcgaaaaat actatgacat gtttccacca
    22261 acaaatccaa atctgactgg aaatgttcat agaaagtcga tgtttgttcc ggtcctattt
    22321 gctggtgaaa gagctcaggt tcctgactgt accagtaatc ccagttattc cacccctttc
    22381 catcaccagc tacacttcct tcactttgag gccctgaagt ggaggatccc cacaaaaaac
    22441 cttctggaaa acgatactct ctttgctcag ttaccattct tctacctact ttctttcatg
    22501 ctacagacgg agttgttaga acctggtttc acaatgcagt ttccacaaaa ttactttgct
    22561 tgtacataca ggctcctgat aaaaactaat ttcccttagt ttaagtatac tcccctaacc
    22621 cgtcaatggt cttccgcaat ctcaaggaaa aactccacaa atcatagcaa agactcaaac
    22681 tactttcttg atgaaaatat gatacaataa aggcatgaat acagtagata ccattattat
    22741 cggagcgggg cctgctggca tgatggccgc catttcttct agtttttatg gcaagaagac
    22801 cctgcttctc gaaaaaaaca agcgtttggg caagaaacta tctggcacag gcggcggacg
    22861 ttgcaatgtg accaacaacg gcacactgga agacttgctg gcaggcattc ctggcaatgg
    22921 ccgctttctg tacagcgtct tttctcagtt tgacaaccat gacatcatga atttctttca
    22981 ggaaaacggg gtcaagctca aggtggagga ccacggccgt gtctttccaa ccaccgaccg
    23041 ctcccagacc attatcaagt gcctggagat gaaaatgttg gaaaatggcg tggacatccg
    23101 tacaggaact gaggtggtgt ccgtccgcaa gatggatgac ctcttccatg tcaagaccag
    23161 cgaggaagtc tttacagctc cccagctgat tgtcaccacc ggtggcaaga cctatccttc
    23221 caccggatcg actggttttg gacacgacat tgcccgccac ttcaagctgg aagtcacgga
    23281 gattgaagcc gctgaaagcc ccgtcctgac cgacttcccc cacaagaagc tccaaggaat
    23341 ttccctggac gacgtcaccc tgacctacgg caagcacgtc atcacccacg acctgctctt
    23401 tacccacttt ggcctgtcgg gacccgctgc tcttcgcttg tccagttttg tcaaaggcgg
    23461 cgaaaccgca tttctcgatg tattgccgac ccattctgac caagacctgt ttgaacattt
    23521 agaaaagaac agggaaaaat ccgttaagaa taccctttct gaactcatgc cagaccgtct
    23581 agcagacttt ttcgctgaaa actacgatag caaggtcaag caagtttccc aaaaagacct
    23641 gactgaactg gtcaccctcc tcaaagtctt acctatcaag attacaggca agatgtccct
    23701 ggccaagtcc tttgtgacca agggaggcgt tgacctcaag gaaatcaatc ccaaaacctt
    23761 agaaagcaag aaggtgcctg gtctccactt tgctggtgag gtcttggaca tcaacgccca
    23821 caccggtggc tttaacatta cctactgcct tgcgactggc tgggtggctg gtagttccta
    23881 ctcttcataa atcaaactca gacgttgttg acttgatttg atgagtgtaa agctccagtg
    23941 gagctttaca gcctgctaca cgaaaataaa aggtacagcg ttctatctac atctaatggc
    24001 acgaactttg ttcccctatc tccaacctcc aaaggttccc cgaacctttt tgtagcgaac
    24061 tttgttcgcc ttatctccaa cctccaaagg ttccccgaac ctttggagct agtctgagtt
    24121 cgattttctt tgagtagtac actactaatt atttaatgac tgaaaaattc aagcaaaaac
    24181 tgcttgaatt tttttgtttt ttattgtatg ataaaaccat aacaatataa caaaggagat
    24241 tggctatgca actgaaactg tcttctattt ctaaaaaatt tgatcacaag agtattctgg
    24301 aggatgccag ttttcaattt gaacaaggca agatttacgg cttgctagga cggaacgggg
    24361 ctggtaagac aaccttgttt aactgcattg cccgtaatct gaccttggat aatggctcga
    24421 ttcagtttgt gaaaggcgaa gaaactcttg actacgataa tacggacatc ggctttaccc
    24481 agacctatcc acaactacct gcctttatga cagcctatga gtttgtccgc ttctatatgg
    24541 acattcataa ggataaactc aaatctcctc gcagtccaga agaatggcta aacttggtcg
    24601 gtattggaga agaagaccag caccgtctgc tcaaggattt ttcccatggt atgcagaaca
    24661 aggtccagct actcctgtcc ctgattgtcc agccacctgt ccttctctta gatgagccgc
    24721 tgacttcctt tgaccctgtg gctgcccacg agttcaaaca gctgattcgg gaggctaaaa
    24781 aggattccgt tattatcttt tcaacccata tcttacaact ggctcaagac ctctgtgacg
    24841 agattgtttt gctccatcac caagaattgc aagcagttcc gagcgacaaa ctccatgatc
    24901 ctgacttcga acaggaagta gttgccttgc tgacagcaga ttaggagaaa cctatgaaaa
    24961 caatattacg ttattcttgg tatcaggaaa aatataacct ccatcggtca gtcaatggat
    25021 tcttctacta tcttcggaaa ctgccgcttg tgggaaagtg cattccagag agtattttca
    25081 agtcttacag ctttaagtct agtctctttt tggtcctgca catcttatcc atccccagtc
    25141 gcttcctgat caagggcttc tggttgctct ttcatttcta ccatgcttct ttctggatga
    25201 atatgctagt caatggggag ctgacctttt ggaaaattct cccaaacact tggctccttg
    25261 gctttatgtt ctggcttttg tttgtcggac taagctatcg tttggggaag agcttagacc
    25321 ctgttatctc taaagcagat cgagaattta tgcagaattt cagcctgtca caatccagct
    25381 ttcttcaaag ccaacttttt atcgagccta tcctgaccac tatttactac ctgccagcct
    25441 caaccatttt ttgcatgata accaaggagt ggttagtttt tcctattggt ttgatgacag
    25501 tgctggcagg tcatctagct ggccatgccc tcaatcgtct gctctttgaa cgtcgtatct
    25561 tttcccgtcg caattcgtgg catgcttggc tatggatcgc gtttgttttt gttttatatg
    25621 ttttggctat tgtcttccgt aatcagctct cctcaggcat gctagtccct atcctgcttg
    25681 tccaaccatt tctgatttgg tttagctatg gttatttgaa gcaacaaacc aaccacctag
    25741 actatctttc ctactgtatg gacgagtcct tgcaaatgga taagaaactt tttgagatga
    25801 ccaagggaaa tgaatacact cgccagggtt tgcagatgca ggcaaaactc aatgcgaaaa
    25861 caggaaagga cctgtcccac ctgtctggca tgacctactt aaatgctctg ctatttgacc
    25921 gctacaaaaa agtcctctat aagaaggtgc agaactgggt cttggcagta gtcgtgattc
    25981 tggcagccct ggaggggatt cgttactttt tagatccgtt tacctttacg gaagcctact
    26041 tgctccaatt cctaccgctt tccttcatga ttatgtacgt agcttcgtcg ggcaaggtgg
    26101 tggctcagat ggtctttgtt aactgcgata tttccatgct tcactatccc ttctatcgag
    26161 aagccaagac tattatcgct ggttttaact accgattttt gcagacctgc aagctcaatc
    26221 tcatctttgc gaccagcctc tttttggcta tcatggtgct ggggcggttt gcctttagtt
    26281 tggaaaccat tctactaact gccctgctcc tcatcagtct gaccgctctc ttttccttcc
    26341 acgatctctt tatctactat atcctccagc cttttaccaa tgacatggat gtggtcaatc
    26401 ccctctataa atttcttagc ggtgctctct actgggtggc ctatctcaac acccgaatca
    26461 atgtaggtag ccacacctat atcctgctgg tttccctagc tatgattgcc tatgttagca
    26521 tcggttactg gattttattg aaaaaagctc cgcagacttt tagattaaaa gcatgaaaaa
    26581 cagcctctcg caagagaagg ctgtttttta ggtatgacga gcatgtcgta tcaaaaaaat
    26641 ccaaagtcga atgactttgg attttggggg ttcgatagat aataactgat acctaccaat
    26701 catagcgagg gaatttcctt gctatgaaag agtatcatac tactatcttc taggaaaatt
    26761 gcggcttcca aaagagttct gaggaagagc ttatcttaag cttcttcgtc tcttcttctc
    26821 tttgctgcaa gaagtgctaa tccgagtcca agagctacta atccgagtga agttgctgaa
    26881 tttgcttcct caccagtatt cggtaattgc ttagctttct tctcaacatt attagcattt
    26941 gtagtaactg atttaacttt cactcctgct gcagaattat tttcgtctac tgctacaact
    27001 tcagcttctg ctgaatcaga actatttcca acctcgtctt tagagtatgc tgtaacaact
    27061 gtacctggct taataacaga acctggaacg attacttgcc cagtcacagg gtccacaatc
    27121 accactgtat tacttgctgt ccataaacca tccgtacctt tcgtaacaac tacagtttgc
    27181 gacttaccat tgttatctac gtatgaaaca acaagctcaa cagctttttc agacggtaca
    27241 atcacaatat tacctttgcc atcatttgcg accgtaggag cagttttatc tgcctgcttc
    27301 acaacttcta ctggtgatag caaatcaata gttccatctg gatatttaat tgtcactgaa
    27361 gcatctttag aaacattaat ctctgttcct tatgggaggt tattgttctc tttaaggaat
    27421 gccttgatac gagcaatttc tttttcagtc aagttatttg gatcaaccac tggtgttact
    27481 ttcgcagctg gtttaattag agctgccatt ggaagtttga ttttttccaa ctcttgataa
    27541 gttctaacac cattgattaa aacaatcgct tcctcaacag ctttatcaat ttcagattta
    27601 gccgcttcct tagctgcatc tgtcaagttc ggattggagt caatgactga cttagcggat
    27661 tctgcctctt tagcaatctt gttcttagca tcttgtttcg cggcatcaag aatgtctttg
    27721 gcgatggcgc ctacgccttt gtcctctgcc gattgcgctt cgactggact tgttgaagca
    27781 tcaattgcat ctgtcgcagc tttagcatct gcatctaccg ctttcttagc tgattccttc
    27841 tctgcatctg tcaagtttgg attcgcgtca atggctgact tagcagcgtc ggattcttta
    27901 gcaatcttat tcttagcatc ttgtttcgct gcgtcaagaa catcttgggc gattgaacct
    27961 acgcctttgt cctctgccga ttgcgcttcg actggttaat agctttttcg cctactgctt
    28021 ctgcactgtc cagattaatc tcaccattag tcttagcatc attgatttct gaggccgtcg
    28081 tagctttatc aattttttct tttgcttctg tcgccttgtt gtctaattct ttcaaagcat
    28141 ccccagcagc tgcagcaacg gcatccttag cagcctgtct ctgttctgct ctcaaggaaa
    28201 tgtcatcatc aattttagaa aacgcttcat cccgttttgc ttcgatagct tttttatgtt
    28261 cagctaattt tgtgtcgatt tctgcttttg cttcattccg tgtatctgcc aagtttgtgg
    28321 tagcattctt aataatgtct gtcgcaccat caacattgac accaccggca ctgtagtcaa
    28381 ctgacacagt accatctgag ttaactgtaa atgctgaatc tggcaagccc ttcaatcttt
    28441 cgttgtctgc gttgtctttc ttgatttgag caataactgc ggctttttct tctggcgtta
    28501 agttagctgg attttcaaca gaaactggtg ttgacaccgt agcatgtttt acgaaggtaa
    28561 cttttacaga tgtcaaacct acattttctg gtttatttcc atcaacaagt ttcccctctg
    28621 gaccaaaagg atagttatct tctccaacac gaagcttata agtgccggta ccttctgtag
    28681 caggtccggt acctgtaatc ttaacgttaa acttagttga agattgaccg tcatctcctt
    28741 ggtttgcagt tgctcccgta ggatccagtt ctgaagtaat cttagtgttt gtacctaagt
    28801 ctgatgatac aagagaacct gactctccat ctttcaaaat tgagagagtt ccatttagtt
    28861 tgccggtatt aaggaaggcg ttatccgtac ctgggacgct aaccgtatac tcttcaccaa
    28921 caaaacggat gtactctgat ttaggtgtca tgactggtgc tttagtatct atgaataaga
    28981 tggtgtaatc taattcgaga gttttagcct ctgtggcatt cgccttatct tttgtctcaa
    29041 cagctttgta agtgaagcca taagtaagat tatttgatcc tgtataagtg gctaccccac
    29101 tgtttgcacc gccagttttt ggttgaatat atccaacaat tccttctttg gcgccatctc
    29161 catcagtatc agttgtcgcc actgcatcat ttcttacaaa ttccaagtta cgtttagcaa
    29221 gctcatctgc tacaccatct ttcaaattga agttttcaat actgaaatcg gatggaatat
    29281 ccttcaatat aactgctgcc ttttctccag cttgaccaat ttgcattata gcatgaattg
    29341 gggcttggtt gtctgtatac ttccgaattt cagcatcctt cgctgtgtcc aacttatagg
    29401 catttacagg aatccagttg tagatagttt gaccgtaaac attggttgtt tttactcgaa
    29461 cttcgtattt accttcagtt gggaaatcag atagatttgt ttcctctact gtttctgttt
    29521 ttgtgccatc agaatttacg gtacgataac ctgcaattga atgtttgtag ttgctagaat
    29581 caattacaag agcgctattt gaagttgttt tagcagactt atcgacaacc aacttatcaa
    29641 aaatagcctg ttccgtcagc tcagaagagt ttgatactgc tagaggttta tcatctactg
    29701 gcttgactgt atatgcaacc atatagaacg gattactctt agtagtatta ccagtggcat
    29761 ccgtcacttt aatccgacga ccagctaggt ctcctgctcc ccaagggaaa cttcctgtta
    29821 gataaccatc ttgtgtgaga gtggctgacc catttggacc atctaagtaa gatactggac
    29881 caggtgaggt gccattagta acaattgagt ttggatcatc caaggtatag cttgcaatat
    29941 cagctggaac ttcgtatgaa atagtttgaa tgtcgctatc tggatctgtg attggaaaag
    30001 ttttagttgc atcatttctg ttaaatgttg gagcaacatt ttgtttacca tcttctgtct
    30061 ccgtagtccc tgctacatta ccaaaaacgt atacaacacg ataaatctta ttatccgttg
    30121 ccttaccatg aacattctga ccactcgcat ctttaggaag atctccaata gtcggaataa
    30181 ctggtgcttg gtttgttaca ctagcattca gcgcctccgg caagatttca gctgcacggt
    30241 tttccatgtc cgtgcgaact gcttcgattg gttccttggg ttcttcttcc gttttagtct
    30301 catttccaag gtcttggtta gctggtgcta cagctgtctc tgcttctgtc actgtgttag
    30361 catgaacaac agggctgttt gcgattgctc ctcccaaaat aacgtttgca atgactgccg
    30421 aagctagacc aaagctaaat ttacgaaaag aaaaacgttg ttcttttctg aacatatctt
    30481 tataagacat gatatcctcc tatattttac tggcgaatgc catcatttct ggttcctgta
    30541 acaccatgta caaaaacac
//
LOCUS       DBNFCK010000009        29821 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.009, whole genome shotgun sequence.
ACCESSION   DBNFCK010000009 DBNFCK010000000
VERSION     DBNFCK010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 29821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29821
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.009"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            33..734
                     /locus_tag="AC1PIW_000337"
     CDS             33..734
                     /locus_tag="AC1PIW_000337"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047465 - N-acylglucosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HIP6605305.1"
                     /translation="MGTISKTDLKQQIKDGIIVSCQALPGEPLYREEGGIMPLLVKAA
                     QEAGAVGIRANSVRDIKEIKEVTTLPIIGIIKRDYPPQEPFITATMREVDELAALDIE
                     VIALDCTKRERYDGLDIVDFIKQIKEKYPEQLFMADISTFEEGLTAYEAGIDFIGTTL
                     SGYTSYSRQEEGPDIELVDRLCRAGIDVIAEGKIHYPDQVKIIHDLGVAGIVVGGAIT
                     RPKEIAERFISALHK"
     gene            762..2270
                     /locus_tag="AC1PIW_000338"
     CDS             762..2270
                     /locus_tag="AC1PIW_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HIP6605306.1"
                     /translation="MNFKKFSQLGQAFMLPISILPVAGLLLGIGGALSNSNAVAQFPV
                     LDQAWLQAVFTIMSTAGSAVFANLALIFAIGVAVGLADADKGTAGLSAGVAYLVYTAT
                     ISGFLTLFADKGASIDTGVLGALAIGITVATLHNKYRKIELPAFLGFFGGSRFIPIIS
                     SFAAIAIGSIFYIIWPPIQNLLVDFGKAIAEMGSFGTFLYGFGLRLTGAFGLHHTIYP
                     MFWYTSLGGSEVVAGETIQGAQNIFFAQLADANHTGLFTYGTRFFAGRFATMIFGLPA
                     SCLAMYHAIPKENRKKVGSFYSSSALTSLLTGITEPIEFSFLFVAPWLYVVHAFLDGL
                     SFLVADLLQIRIGNSFSGGLIDYLIFGVFQGNDKTNWIYVIPVGLLWAAVYYLVFKFL
                     VTKFHVLVPGMESSDQASHSEKVLSNGSSLRDNAIAIIDALGGADNIENVTACATRLR
                     VSLRNSGLVDKGVIQSIGATAVLDVKGGIQAIFGGKAILYSQEINQILGLEE"
     gene            complement(2374..3186)
                     /locus_tag="AC1PIW_000339"
     CDS             complement(2374..3186)
                     /locus_tag="AC1PIW_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIP6605307.1"
                     /translation="MLQTPKHITAIIESHLDQMTRLEKQIASYFTQLDPSSADLSQEN
                     TIQQLHISPSALTRFAKKCGFSGYREFVFAFQNNQQYLDKHFEKLQRSLTKKVLVDYE
                     EILTATNKLVDEEKLEEIAKLIDSSKRVYFYGIGSSGLVAMEIKSRFMRLGVVCDAIT
                     DENNLIWTTNILDDSCLVIGLSLSGQTEEVMEHLQLAAQKGIPTALLTTRSFPHPTFD
                     QIIPVASVRHLNYGNRISPQIPLLIMLDIIYAYFLAIDKEKKENIFKQTIRE"
     gene            3286..3783
                     /gene="ybeY"
                     /locus_tag="AC1PIW_000340"
     CDS             3286..3783
                     /gene="ybeY"
                     /locus_tag="AC1PIW_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIP6605308.1"
                     /translation="MYIEMIDETGQVSAQMQEQITELLQFAAEKIGKQNKEMAVTFVD
                     NKRVHEVNLEYRGIDRPTDVVSLEYKPESEIVFDEEDLLDNPELAEMMEDFDAYIGEL
                     YISIDKAREQAEDYGHSYEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRE"
     gene            3764..4165
                     /locus_tag="AC1PIW_000341"
     CDS             3764..4165
                     /locus_tag="AC1PIW_000341"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HIP6605309.1"
                     /translation="MDLQENNSKNRWKNRELIASLEFAVTGLLTAFKEERNMKKHLAS
                     AVLVVLAGLIFQVSVTEWLFLLLSISLVIAFEIVNSAIENVVDLASDYHFSMLAKNAK
                     DMAAGAVLFVSGFALLTGLIIFVPKIWDFIF"
     gene            4182..5081
                     /gene="era"
                     /locus_tag="AC1PIW_000342"
     CDS             4182..5081
                     /gene="era"
                     /locus_tag="AC1PIW_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HIP6605310.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNYVMGQKIAIMSDKAQTTRNKI
                     MGIYTTEEEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEKRGKGD
                     DMIMERLKQAKVPVILVVNKIDKVHPDQLLEQIDDFRQQMDFKEIVPISATQGNNVNR
                     LMEILKENLDEGFQYFPADQITDHPERFLVSEMIREKVLHLTREEIPHSVAVVIESMK
                     RDEFTDKVHIRATIMVERDSQKGIIIGKQGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEKEY"
     gene            5694..6521
                     /gene="mutM"
                     /locus_tag="AC1PIW_000343"
     CDS             5694..6521
                     /gene="mutM"
                     /locus_tag="AC1PIW_000343"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HIP6605311.1"
                     /translation="MPELPEVETVRRGLNRLVKGKVISKVEVTYAPMIKTGVDAFCQD
                     LIGQEILDVDRRGKYLLIYLTDHVLISHLRMEGKYNFFADQVPANKHFHAFFTFIDGS
                     TLVYQDVRKFGTMELLGKADVDAYFISRKIGPEPTEEDFDLEEFAKKLAKSKKPIKSH
                     LLDQSLVAGLGNIYVDEVLFKAKVHPAQTSNQLSTDQVADLRQATIEVLQLGIEKGGS
                     TIRTYKNALGMDGTMQDYLQVYGKTGQACPRCQTEIVKIQLGGRGTHFCPKCQVKHG"
     gene            6514..7116
                     /gene="coaE"
                     /locus_tag="AC1PIW_000344"
     CDS             6514..7116
                     /gene="coaE"
                     /locus_tag="AC1PIW_000344"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637643.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIP6605312.1"
                     /translation="MAKIIGLTGGIASGKSTVTAFLREQGYPVIDADAVVHELQAKGG
                     KLYQILLKEFGPDILSADGHLDRVKLGQAVFADSKLRARLSDLQDQIIRQELLDRRDA
                     LKQTEQVLFMDIPLLYEADYSGEVNEIWLVYVDRAQQLERLMKRNGLAVQDAENRLAA
                     QLSLEEKRSQAQVVIDNSGAVEATLAQVAQLLEELKDGRR"
     gene            7103..8308
                     /locus_tag="AC1PIW_000345"
     CDS             7103..8308
                     /locus_tag="AC1PIW_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HIP6605313.1"
                     /translation="MDDGSSYWKQNLKVAWLGNFLTGTSFTLVMPFISVFVEELGVGP
                     GQVEYYAGLAVSVNALAAALMAPIWGSLADRYGRKPMMVRAAFAMIFTMGGMAFVPNV
                     FWLLTLRVLNGVFTGYIPNATALIASQVPKDKTGYALGTLSTGAVAGNLIGPTLGGIL
                     AEMFGVHMVFLLVGLLYAIVVLLTVFYIREDFVPVKKGEEMSVKEVFEQVKDRQMLVG
                     LFVTSMIIIAAAQAVVPILTLYVRHLGQTDNLLFVAGFIISLPGMASLVTSGYLGKIG
                     DRIGNHRLLLIALTYSLLINVFCVFAENPFQLGLLRFMYGFGTGALLPSVNSLLTKLT
                     PKEGISRIFSYNQLFNNLGSVVGPMMGSAVAAHMGYDWVFYLSSGLVLFNLIWSLTNF
                     RNYLKVRDV"
     gene            8308..8457
                     /gene="rpmG"
                     /locus_tag="AC1PIW_000346"
     CDS             8308..8457
                     /gene="rpmG"
                     /locus_tag="AC1PIW_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIP6605314.1"
                     /translation="MRVKINLQCSECGSKNYLTSKNAKTHPDKIEVLKYCPKERKVTL
                     HLETK"
     gene            8504..8737
                     /gene="secG"
                     /locus_tag="AC1PIW_000347"
     CDS             8504..8737
                     /gene="secG"
                     /locus_tag="AC1PIW_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIP6605315.1"
                     /translation="MYQALLAILLILSVILIVVIFIQPAKNQSSNVFDSSSGALFERT
                     KARGFEAVMQRITAILIFLWMLDALALVIISSK"
     gene            9169..11538
                     /gene="rnr"
                     /locus_tag="AC1PIW_000348"
     CDS             9169..11538
                     /gene="rnr"
                     /locus_tag="AC1PIW_000348"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HIP6605316.1"
                     /translation="MKNEIINYIKEVGPVTMDQLADQFGASSAKSFTDLVKLVSSMEG
                     SRQLVFDNAGRITLPAPKISKNKVTLQGIFRAHKSGFGFVTIDEEEDDLFVSRDDVNF
                     AIEGDRVEVAIKKVADRFKGTAAEAEVIDILEHSLKTAVGLIVFDEDKPEYAGYIKSK
                     NQKIAQKIYIKKSPLVLTGTEILKVDIEAYPNKKRDHFVATIRDVVGHKDDVGIDVLE
                     VLESMDIVSEFPDEVLAEANRVPESPSEKDFEGRLDLRDEIIFTIDGADAKDLDDAVH
                     IKQLKNGNLELGVHIADVSYYVTEGSALDQEAVKRGTSVYVTDRVVPMLPERLSNGIC
                     SLNPNVDRLTQSAIMEIDCKGKVVKHWIGQTVIKTTFRMTYSDVNDMIAGNKEKLDKY
                     KTIVPSVELMVKLHETLETMRYKRGALNFDTAEAKIIVNKDGLPVDIQLRQRGIAERM
                     IESFMLAANECVAEHFAKLDLPFIYRIHEEPKSDKLQKFIDYATSFGLTVYGTASSIS
                     QDALQDLMERVKDEPYADVLNMMLLRSMQQARYSEHNHGHYGLGAEFYTHFTSPIRRY
                     PDLLVHRMVREYGHNPSEKAEHFEQVIPELAKSSSSLERRAIDAEREVEAMKKAEFMQ
                     EFVGQEFDGVVSSVVKFGLFVELPNTVEGLIHVTNLNEYYQFHERTLTLQGEKSGRVF
                     RVGQPIRIKLTRADKMTGEIDFAHMPSELDIVEKALKAKRRTASHSNRDRDDRSGRGR
                     GKHYKQEAAGRDSGRHKSGKDHKSKSGKKDKKKKPFYKEVAKKNKKKRR"
     gene            11541..12008
                     /gene="smpB"
                     /locus_tag="AC1PIW_000349"
     CDS             11541..12008
                     /gene="smpB"
                     /locus_tag="AC1PIW_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIP6605317.1"
                     /translation="MAKGEGNVIAQNKKARHDYTIVDTVEAGMVLTGTEIKSIRAGRI
                     NLKDGFAQIKQGEAWLVNVHIAPYDEGNIWNQEPTRTRKLLLRKKQIESLDNEVKGTG
                     MTLVPLKVYIKDGFAKVLIGLAKGKHDYDKRESIKRREQDRDIKRTMKAINSR"
     gene            complement(12095..13138)
                     /locus_tag="AC1PIW_000350"
     CDS             complement(12095..13138)
                     /locus_tag="AC1PIW_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIP6605318.1"
                     /translation="MKAAIYEKAGKMTVAEIEKPSLQAKDDVIIKVVRACVCGSDLWG
                     YGEDIHHDHGDTNSGHEAIGIVEEVGEDITTLVPGDFVIVPFTHGCGHCDACRAGFDG
                     TCDNHPGYTNWGNNYQSQYIRFHYGNWALVKIPGQPSDYSEGMIKSLLTLADVMPTGY
                     HATRVADVKPGDKVVVIGDGAVGQCAVIAAKMRGASQIIMMSRHADRQQMALESGATA
                     VVAERGEEGIAKVREILGGGADAALECVGTEASIDQALGVLHNGGRIGFVGVPHYNNH
                     ALGSTFAQNIIIGGGSASVTTYDKEILLDAVLKGDINPGRVFTQTYSLDNIDQAYKDM
                     ANRKTIKAMITVD"
     gene            13307..14167
                     /gene="tehB"
                     /locus_tag="AC1PIW_000351"
     CDS             13307..14167
                     /gene="tehB"
                     /locus_tag="AC1PIW_000351"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HIP6605319.1"
                     /translation="MTVLVPYKRMPIWNQDTVPQHFLTKHNTKVGTWAKIKVLRGQLK
                     FEPISDDNEILGEFVYGPTDDVPFVEPQAWHRVTLLTEDTEFFLEFFCQPEDYFAKKY
                     DYTRTHSEVLEALKRIEPCKVLDLGCGHGRNSLYLAQQGFEVTAVDKDVPSIRTLLQV
                     KEVEDLDLQAGTYDINSASLEQDYDWIISTVVFMFLERERVPAIIRNMQEQTRPGGYN
                     LIVAAMDTEDAPCPMNFSFTFGVGELKEYYRDWELVKYNEDFGQLHKRDENGHFLKMR
                     FATMLARKKG"
     gene            14361..16184
                     /locus_tag="AC1PIW_000352"
     CDS             14361..16184
                     /locus_tag="AC1PIW_000352"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HIP6605320.1"
                     /translation="MKKTFVRIATFLMLVCLFPVQLVQACSGFIIGKGLTTDGSILYG
                     RTEDYPYPPNNGAHNKNYIVVPATAYAKGDMLVDESFGFTAPHLANEFKYTSTPDAAR
                     GDGSNGNFGAHGFNEKGVSMTATVTAIPNKKVLAVDPLVTAGGLGEAILIDYVLPRVT
                     SAREGIELIAKTIDEKGSAEGNIIVIADKNEVWYMEILSGHQYVAIKFPEDKYAIFAN
                     TYYLGHVDFTDTENVIASAKVEEVAKQAENYMMVDGKFHIAKSYGPENYADGDRSRTY
                     AGIKLLDPASSVTYEDAVYDLLRQPTDPSRRFSLQDTFALQRNRFEHLPEFRPDDEAG
                     KVKQGDNGANDQAADATYKYALGNENVIDAHVYQINSSLPSAFGGTVWLGLAQTRNTP
                     YVPFYGIVTDTYEAFKNRSASYDTNSWYWTVQNIDKMAISHPELFGTSIQEKWIALEK
                     EWIASQAALDAQYAGLSEDAAVALAPTVTEATLARSAEIFAQLKAVEAEMMAKIEAAT
                     TPSSSSTEPSTSTEPSSSGTETSTSTSQSTSDSNTGGATDTSSSSRTVVPSDKKVTPT
                     NKKGKSSLPSTGEQVSLLLVALGVAGILTAIFLHRKKSSKE"
     gene            16515..19004
                     /locus_tag="AC1PIW_000353"
     CDS             16515..19004
                     /locus_tag="AC1PIW_000353"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140358 - P-type transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIP6605321.1"
                     /translation="MKQASYPIQGMTCASCALTVEKAVGKLAGMEEVSVNLATEKLSV
                     SYDEKLLGLEDIRQAVEKAGYQLVDNLVTESYDISGMTCASCALTIEKALGKLEGVEE
                     VSVNLATEKATIRYSRHRQNPASLERAVEQAGYQLIRPEKVEGAADKGPSTEEKLRHR
                     FVWSAAFTFPLLYIAMGPMLPWGGLPLPALLHQPLVYAISQVILLIPILYIGRSFFQK
                     GFKTLLQGHPNMDSLIAVGTGAALVQGLLMIVFLQMGKEVAMHGHHPELYFESAAVIL
                     TLITLGKYFEARAKGQTSEAIKKLMDLAPKAAQVLRNGQEMQVPIEEVVVGDQVIVRP
                     GQQIPVDGQVLEGQTRVDESMLTGESLPVKKALGNNVFGGTLNQQGAITMQATKVGRD
                     TTLAQIIRLVEEAQGSKAPIAKLADQVSAIFVPVVMGLALLSGLAWYFLGQESWIFSL
                     SIIIAVLVIACPCALGLATPTAIMVGTGKGAENGLLFKSGQAIETLQGVNTIVFDKTG
                     TITEGKPQVTDIHLLSTKNREQVLQLAASSEQFSEHPLAQALLQAAQTEKIELLPATD
                     FQALSGRGLSVIIAEQTIYLGNERLMREQGIDVSKGRAVAEAFAQQAKTPVFLASQQE
                     VLAVIAIADKIKETSRQAVQALQTIGLEVVMLTGDNEKTAKAIAKEVGIEQVISQVLP
                     DDKANQVKFLQEQGKTVAMVGDGINDAPALAQAHVGLAIGSGTDIAIESADIVLMHSD
                     ILDVVKAVKLSQATMRTIKQNLFWAFAYNVIGIPIAMGLLHAFGGPLLNPMFAGAAMA
                     LSSVSVVLNALRLKTYKLYQKYQSKNWYFFT"
     gene            19044..19529
                     /gene="tpx"
                     /locus_tag="AC1PIW_000354"
     CDS             19044..19529
                     /gene="tpx"
                     /locus_tag="AC1PIW_000354"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HIP6605322.1"
                     /translation="MTTFIGKPVTLVGPQLRVGDVAPDFVLMANDLSLKSLKDFGKQT
                     KVISVVPSIDTGICDTQTRRFNQELADRDNTVVITVSADLPFAQKRWCGAAGLEDAIT
                     LSDYYDHAFGKSYGVLMREWNLLARAVFVLNADNEITYVEYLDNVNEHPDYDAALEAV
                     K"
     gene            19624..19959
                     /locus_tag="AC1PIW_000355"
     CDS             19624..19959
                     /locus_tag="AC1PIW_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HIP6605323.1"
                     /translation="MEKTQIIQDVLALIQDLNRAFQADKVGTADEQRFQHNLTETTRI
                     LSEAKSVTNTELIAIEKFYRSTSFLVGLGDLRLSETSRQAWRAFDRYYYETIREELKL
                     YGGSAIAQV"
     gene            20048..20749
                     /locus_tag="AC1PIW_000356"
     CDS             20048..20749
                     /locus_tag="AC1PIW_000356"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6605324.1"
                     /translation="MSYIQVKNSSKYYQMGDATIVANDGVSFEIEQGELVIILGSSGA
                     GKSTLLNILGGMDSNDEGEVWIDGVDIAKLSSHELTNYRRDDVGFVFQFYNLVANLTA
                     KENVELAAEIVKDALDAEEVLEQVGLGHRINNFPAQLSGGEQQRVAIARAVAKKPKIL
                     LCDEPTGALDYQTGKQVLQILQDMSRKQGATVIIVTHNSSLAPIADRVIRMRDARVHS
                     VELHAEPQDIASLEY"
     gene            20761..24126
                     /locus_tag="AC1PIW_000357"
     CDS             20761..24126
                     /locus_tag="AC1PIW_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIP6605325.1"
                     /translation="MKKKIYWKDMRQSLLSSKGRFLSIFSLMMLGALALTGLKVTAPN
                     MEKTAQAYIASHRTMDLAVISGLGLSQADLDELETIEGATLEAGYFKDVVTNEGQTAI
                     RLFSAPKTLSTYKLVEGEVPSQKGQIALSASFKNRYKLDDTVQVTESDKDGKILTQTS
                     FTLVGFVASAEIWDNETMGMAVSGDGQLGGYGLVSQDTFKSEVYTIARIRYDDLVDLP
                     YYSQAYQDRLDQHQEDLEKLLANNDEQRLATIQSDGQTDISKGEEEIAQAQSQLEQAE
                     SELETGEEQLATGRSEFARGRAKVVQTEAELRAAQAQLLKNKFELEESKKELDGRKAQ
                     LEQTASFLDGYQMELEMSAQQLEEAKQELDSQNAQLSQTASQISTGRASWFQAQQELD
                     LEIANHLQEGQTLSDYPDLLARQEGLDVEKSRLDQMESAHEQANQAYLQGYDYYQTKQ
                     NEYNSNLAQYQTWNQEYQAGLARYQAGLSQYEQGIAAYNKGVEDYEWGLSQLESSNQL
                     LRQEELRLEEADKELSQAQSQFSDKKAVADQEISQAQTEIAQAKSDLSKLAKAPYQVY
                     TRSSLPGGDGYTTYSNATRSIAAVGNVFPVVLYLVAALVTFTTMARFVDEERTQSGIL
                     KALGYTNRQIMAKFILYGLAAGLVGTIVGIIAGNLLLSPLISDIITQTTVIGPAKLHF
                     YPLWTGLALLLSLVSSVLPAYLVARRELTEKPAQLLLPKPPVTGSSIWLEKWPAIWSR
                     LSFTHKVTARNIFRYKLRMLMTIFGVAGTVALLFGGLGIRSSISGVVQRQFGELIHYD
                     MLVVENSRATEEELDKLTHFLQSDQVRQSLPVAFEQLNQTVEENGQRKNLSISLYISD
                     RRDFGNQVSLESSTGQPIKLSDRGIVLTEKLAQIYGVSVGDKLSLTLEDKEVSVRVEA
                     VADMYAGHFIYMTDSYYEQVTGKQKTANAYLVQLKDSQLGHIQTLASQLLAMPAVRSL
                     VQNTSLIDMLTTIAGSLQTIMTILVILSILLGLVILYNLTIINMSERIRELSTIKVLG
                     FHNREVTMYIYRETIALSLIGMLVGLVGGIYLHKLLLAMIGSDSIRFNPSVGLEVYLI
                     PILAISGILSVLGWYVNHHLRKVDMLVALKSVD"
     gene            24296..25603
                     /locus_tag="AC1PIW_000358"
     CDS             24296..25603
                     /locus_tag="AC1PIW_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605326.1"
                     /translation="MKKISDLGLTGRKLVGEGLILVFIGIGFLIAGWQFPGLILRFVH
                     AGLFFLALYELSMTFFRKKKSSESVIALVGKAVLFGILASLDLAVQIPLYFAAIFVGI
                     YQLFTALINFITFYLYRKDGVQPRIRYLIDGIWLFLLGSASLFVSGTQLVVQTIVIGG
                     YLILYGLTNLRDGFLFEEAIEQQNLKRHVRLPLPLFLAALIPRMTLQKVNDYLADNEG
                     QTAQSIYNRHKEIAELPALEVFVHVGEEGFGAVGHVDLSYKGRVYGYGSYDVLSERLG
                     GAIGDGVLFKAERQAYIDFCNQEGMTMLGYQLSLRPEQEEAIETRLAEIDNLLLPWNP
                     SPEKVSKTVDGQPIEMYAYRMKEAIHAELFKFRKSKFKTYFVLSTNCVLLADSVIGQA
                     GTDILGMHGFIAPGTYQSYLDQEFEKPHSLVVAKNIYYRKEKS"
     gene            25679..28315
                     /gene="polA"
                     /locus_tag="AC1PIW_000359"
     CDS             25679..28315
                     /gene="polA"
                     /locus_tag="AC1PIW_000359"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIP6605327.1"
                     /translation="MKKNKLLLIDGSSVAFRAFFALYNQIDRFKNANGLHTNAIYGFN
                     LMLDHMMKRIEPTHILVAFDAGKTTFRTEMYADYKAGRAKTPDEFREQFPFIRQMLDA
                     MGVKHYELDQYEADDIIGTLDKMAERTDIPFDVTIVSGDKDLIQLTDENTVVEISKKG
                     VAEFEEFTPAYLMEKMGISPTQFIDLKALMGDKSDNIPGVTKIGEKTGLKLLTEFGSL
                     DGIYENIDSMKASKMKENLIADKEKAFLSRTLATIDTQAPIEIGLDDIVYQGPKVDEL
                     GQFYDDMGFKQLRAQLGTTSSQEEAVLDFQIVTEISPAMLKQDQFFYFEILGENYHRE
                     DLVGLAWGDKEKIYVGGPELLDSPVLRDFLESQTIKTYDFKRGKVLLDRKEIKLPPAT
                     FDSRLAKYLLSTVEDNSLTTIANLYGQTSLVPDEAVYGKGAKLDLPEREIFFPHLARK
                     VQVLIETEEPMLTKLEENQQLDLLFDMELPLANVLAKMEIAGIKVEAETLKAMQSENE
                     VLIDQLTKEIYELAGQEFNINSPKQLGTILFEDMGLPLEYTKKTKTGYSTAVDVLERL
                     APIAPVVSKILEYRQITKLQSTYVVGLQDAILEDGKIHTRYVQDLTQTGRLSSTDPNL
                     QNIPVRLEQGRLIRKAFVPSLENSVLLASDYSQIELRVLAHISQDQHLIEAFQQGADI
                     HTSTAMRVFGIEKAEYVTPNDRRNAKAVNFGVVYGISDFGLSNNLGITRKEAKAYIDT
                     YFERFPGIKNYMETIVREARDKGYVETIYKRRRELPDINSRNFNVRNFAERTAINSPI
                     QGSAADILKVAMINLDKALTEAGLATRMLLQVHDEIVLEVPVAELETVKAMVKETMES
                     AISLSVPLIADENEGSTWYEAK"
     gene            28302..29084
                     /locus_tag="AC1PIW_000360"
     CDS             28302..29084
                     /locus_tag="AC1PIW_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HIP6605328.1"
                     /translation="MKQSKKGKSMKKLILCIGLCSLLLAGCGKASQKTEKTPASSGEE
                     FSTTLPILQEKQDTTNEQFNVLANAEVVAVNSTPPADETQKGHKIYAANNGVAEKDEN
                     GNIKEYFRYYDVPSTWTINAENTKDETVAAVYDVKEGGSNYMVQIYNINAFNQSPLED
                     GRNMTPEELEARMVETNHSFSEKTVVTINGQEWQVGRQLLTDQKLARLTFYRMESTAA
                     YDDSVVVGSIYYSLDPGDDKKRTNLKKVIGQVKDVVYQIAKK"
     gene            29204..29641
                     /locus_tag="AC1PIW_000361"
     CDS             29204..29641
                     /locus_tag="AC1PIW_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIP6605329.1"
                     /translation="MSYSFQNPSQDIIFDYLKNAKTIAVVGLSSREETAAYRVSKLMQ
                     EAGYKIIPVNPKAAGGTILGELVYSSLAEIDQPIDIVDVFRRSEFLPEVAQEFIQSNA
                     KVFWAQLGLESQEAEKQLRQAGRNDIVMNKCIKIEYLEMKEQY"
BASE COUNT         8498 a         5863 c         7184 g         8276 t
ORIGIN      
        1 atctctagta tggagcagaa aggaatgaca taatgggcac tattagtaag acagatttaa
       61 agcaacaaat taaggatgga attatcgttt cctgtcaggc tttgccaggc gaacctctgt
      121 atagggaaga aggcggtatt atgcctttac tagtcaaggc tgcccaagaa gcaggggctg
      181 tcggtattcg ggccaatagt gtacgagata ttaaggagat aaaagaagta actactttac
      241 caattatcgg aattatcaag cgagactatc ctccacaaga gccctttatt acggctacga
      301 tgcgagaagt agacgagtta gcagctctag atattgaagt cattgctctt gattgtacca
      361 agcgtgagcg ttatgacgga ctcgatattg ttgatttcat taagcaaatc aaggaaaaat
      421 accccgaaca actttttatg gcagatattt caacctttga ggaaggatta actgcttatg
      481 aagcaggcat cgatttcatt gggacaacac tttcggggta tacatcctat agtaggcagg
      541 aggagggacc ggatattgaa ttggttgacc gcttgtgccg agcaggtatc gatgtgatag
      601 cggaaggaaa aatacactat cctgaccaag taaaaattat tcatgattta ggtgtggcag
      661 gaattgttgt aggcggtgcg atcacacgtc caaaggaaat cgcagagcgg tttattagtg
      721 ctttacataa ataataaata ggttatagaa aggaatggag tatgaatttt aagaagtttt
      781 ctcagctagg tcaggccttt atgctgccaa tctcaatttt gcctgttgct ggtctcttac
      841 tgggaattgg aggcgcttta tcaaatagta atgctgtagc acaatttcct gtattggatc
      901 aagcgtggtt gcaagctgta ttcactatca tgagtacggc aggtagtgcg gtatttgcga
      961 atttagcatt gatttttgca attggtgttg ctgtaggttt ggcagatgct gataagggga
     1021 ctgctggcct atctgctggg gttgcatact tggtttatac ggctactatt agtggatttt
     1081 taactctttt tgctgataag ggagcaagca ttgatactgg agttttgggc gctttagcga
     1141 ttggtatcac tgttgcaact ttacacaata aatatcgaaa aattgaatta ccagcctttc
     1201 ttggtttctt cgggggctct cgttttattc caattatttc atcatttgca gcaattgcga
     1261 ttggctccat tttttatata atttggccac cgattcagaa tttattagtt gattttggta
     1321 aggcaatagc agagatggga agttttggaa ccttcttata tggttttgga ttgcgtttga
     1381 caggtgcatt tggcttgcac cataccatct atccaatgtt ttggtatact tcattaggtg
     1441 gaagtgaagt ggtagcaggt gagacgatcc aaggtgcgca aaatattttc tttgctcaat
     1501 tagcagatgc gaatcacaca ggcttattta cttatggaac tcgatttttt gctggtcgat
     1561 ttgcaacgat gatttttggt ctaccagcat cttgtttggc tatgtatcac gcaattccta
     1621 aggaaaatcg taagaaggta ggcagttttt attcaagtag tgcattaact tccctattga
     1681 caggaattac tgaaccaatc gaattttctt tcttgtttgt tgctccatgg ctatatgttg
     1741 ttcacgcatt tttagatgga ctatcattct tagtcgcaga tctcctgcaa attcgtattg
     1801 ggaactcatt ttctggtggg cttattgatt acttgatttt tggtgttttc caaggaaatg
     1861 acaaaaccaa ttggatttac gtgattcctg ttggcttgct ttgggcagca gtttattatc
     1921 tagttttcaa atttttagta accaaatttc acgtgctagt tcctggtatg gaaagcagtg
     1981 accaagcatc acattcggaa aaagttttat caaacggttc tagtcttcgt gataatgcaa
     2041 ttgctatcat cgatgctctt ggtggagcag ataatattga gaatgtgact gcctgtgcga
     2101 ctcgattaag agtatcgctc agaaatagtg gtcttgtgga taagggagtt attcagtcta
     2161 taggtgcaac ggctgttttg gatgtaaaag gaggaataca ggctattttc ggaggtaagg
     2221 ctattctgta tagccaagaa atcaatcaaa ttcttggtct tgaagaatag agttatattt
     2281 aagcgaatta aaagcattca aactgtacct atcgggtatt tttgaatgct ttttgtttta
     2341 ttaagattgt tgtgaaatag aaagacggat gaattattct ctaatggttt gcttgaaaat
     2401 attctctttt ttctccttgt caatagctaa aaaataggcg tagataatat ctagcatgat
     2461 gagcaggggg atttggggtg agatacgatt gccgtaattg aggtggcgga cagaggcgac
     2521 aggaatgatt tggtcaaaag tcggatgggg aaaactccta gttgttaaca gggcggtagg
     2581 aatccccttt tgagcagcca gctggagatg ctccatgacc tcttctgtct gaccagacag
     2641 agaaagaccg ataaccaggc aactatcatc cagaatattg gtagtccaga tgagattgtt
     2701 ttcatcagta attgcatcac agactacacc cagtcgcata aagcgggatt tgatttccat
     2761 tgcaaccaaa ccagaactgc cgattccgta aaaatagaca cgcttgctac tgtcaatcag
     2821 tttggcaatc tcttctaatt tttcctcatc taccagttta ttggtagcag ttaggatttc
     2881 ctcatagtct accaagactt tttttgttag gctccgctgt aatttttcaa aatgtttatc
     2941 caagtattgt tggttattct gaaaggcaaa gacaaattct cgataaccgg aaaatccaca
     3001 ttttttggca aaacgggtaa gagcagatgg agaaatgtgg agttgctgta tagtgttttc
     3061 ttggctaaga tcggcgctag atgggtctaa ctgagtaaaa taactggcaa tctgcttttc
     3121 aagtctagtc atctgatcca gatgagactc aatgatggca gtaatgtgtt tcggtgtttg
     3181 taacattttc cattcctctg atagcttctg aggacattat accactaaaa aaccctcatt
     3241 gataggtttt atgatataat ggaaacgtaa aaggaatgta agattatgta tattgaaatg
     3301 attgatgaaa cagggcaggt ttctgctcaa atgcaggagc agattacgga attgctccag
     3361 tttgctgccg aaaaaattgg caagcagaat aaggaaatgg ctgtgacctt tgtggacaat
     3421 aagcgtgtcc atgaggttaa cttggagtac cgaggaattg accgtccgac agatgttgtc
     3481 agtttagaat ataagccaga gtcggagatt gtttttgatg aggaagactt actggacaat
     3541 cctgaattgg ctgaaatgat ggaagatttc gatgcctata ttggtgaatt gtatatttct
     3601 atcgataagg cacgtgagca agctgaagat tatggtcata gctacgagcg cgaaatgggc
     3661 tttttagctg tacacggttt tctgcatatc aatggctatg atcattatac gccagaagaa
     3721 gaagcagaaa tgtttggttt acaagaagaa attttgacgg cctatggact tacaagagaa
     3781 taattcaaaa aatcgctgga aaaaccgaga attgattgct agtctagaat ttgcagtaac
     3841 aggtttgttg acagctttca aggaagaacg caatatgaag aagcacctgg cttcagctgt
     3901 ccttgttgtg cttgcgggac ttattttcca agtatctgtg acagaatggc tcttcctctt
     3961 gctcagtatt agtctagtta tcgcttttga aattgtgaat tcggctattg agaatgtggt
     4021 ggatttggcg tcagactatc atttttctat gttggcaaag aatgcaaaag atatggcagc
     4081 aggggcagtc ttgtttgtgt caggatttgc cttgttgaca ggattgatta tctttgtgcc
     4141 gaaaatttgg gactttattt tttagaatat aaggagaata catgacattt aagtcaggtt
     4201 ttgtggcgat tttaggtcgt ccaaatgttg ggaaatcaac atttctcaac tatgtaatgg
     4261 ggcagaaaat tgccatcatg agcgacaagg ctcaaaccac ccgtaacaag attatgggca
     4321 tttatacgac agaagaagag cagattgtct tcattgacac accaggaatc cacaagccta
     4381 agacagcctt gggtgacttt atggtggaat ctgcctatag cacccttcgt gaagtggata
     4441 cggtcctctt tatggtgcca gccgatgaaa agcgaggcaa gggtgacgac atgattatgg
     4501 aacgccttaa acaggccaag gttccagtca ttttagtggt taacaaaatt gacaaggtcc
     4561 acccagacca gcttttggaa cagattgatg atttccgtca acagatggac ttcaaggaaa
     4621 ttgtgccaat ttctgctacc caagggaaca atgtcaaccg actcatggaa attctcaagg
     4681 aaaacctgga cgaaggcttc cagtatttcc cggcggacca aatcactgac cacccagaac
     4741 gtttcttggt atctgagatg attcgggaga aggttctaca cctgactcgt gaggaaattc
     4801 cgcactctgt ggccgttgtc atcgagtcca tgaaacgtga tgaatttacc gacaaggtcc
     4861 acattcgcgc tacgattatg gtagagcgtg atagccagaa aggcatcatc atcggtaagc
     4921 aaggagccat gctcaagaaa atcggctcca tggctcgtcg tgatattgaa ctcatgctag
     4981 gtgacaaggt cttcctcgaa acctgggtca aggtcaagaa aaactggcga gacaaaaaac
     5041 tcgacctcgc cgactttggt tataatgaga aagagtatta aaaacgtccg ggggacgttt
     5101 ttaacccgag cttggaaact agagagcgag gaagttcgag ggagtgaaac agtctgtgaa
     5161 tagactgttt cagctggtca catagaaatg ttaaagtggc catttgaaaa aaccgagctt
     5221 gaaaactaga gggcgaggta gttcggggga gtgaaacaat ctaggaatag actgtttcag
     5281 caggtcacat aggaatgtga aagtgaccat ttgaaaaccc gatcttagaa attggaaggc
     5341 gagatagcct ggggataggc tgtctaagtc aatcacttag aaatagttag cagaaaagcc
     5401 gaaaggcttt tactgcgtta tggagaaatc tttttagtaa acaagaaagg aaagccgatt
     5461 aggcgttgtc ttgaactcgc ctacgactct ttgaaaatca caaccgtgac ctaggtgccg
     5521 aagcaccgtc ggtcaggttt ctatttgctt tgtaattttg ttagaaagga tgagtgtttg
     5581 cgtacttgaa cccgagctaa aagctcggaa aatagataga gctccttgtg tgcaagtcac
     5641 acagcgtcgc tctcctaatt ttcagtcgct ttttacgctc ttggtatcgt aaattgcccg
     5701 aattgcctga ggttgagacg gttcgtcgtg gtttgaatcg tttggtcaag ggaaaagtta
     5761 tttccaaggt ggaggtgacc tatgctccca tgattaagac aggtgtggat gccttctgcc
     5821 aagacttgat tggtcaggag attttagatg tggaccgtcg tggcaagtat ctcttgattt
     5881 acctgacaga ccatgtcctc atttctcatt tgcggatgga aggtaagtac aatttctttg
     5941 ctgaccaggt gcctgccaac aaacatttcc atgccttttt tacttttata gatggctcga
     6001 ccttggtcta tcaagatgtc cgcaagtttg ggaccatgga actgctgggt aaggcagatg
     6061 tggatgccta ttttatcagt cgaaaaattg gtcctgagcc gacggaggaa gattttgact
     6121 tagaagaatt tgccaaaaag ttagctaagt cgaaaaagcc cattaagtcg catcttttgg
     6181 accagtcttt ggtggctggt ttgggcaata tttatgtcga tgaggtcttg ttcaaggcca
     6241 aagttcatcc tgcacaaacg agtaatcagc tgtcgacaga tcaggtggca gacctccgtc
     6301 aagccaccat tgaagtcctg caactgggta ttgaaaaagg tggttcaacc attcggactt
     6361 acaaaaatgc cttgggcatg gatgggacta tgcaggatta tttacaagtt tacggaaaga
     6421 cagggcaggc ctgtccccgt tgccagacag agattgtcaa aatccagctg ggcggacggg
     6481 ggacacattt ctgtcccaag tgtcaggtga aacatggcta aaattatcgg tttaacaggc
     6541 ggaatcgcct ctggaaaatc aacagttaca gcttttctga gagaacaagg ttatccagtc
     6601 attgatgcgg atgcggttgt tcacgaatta caggccaaag gtggcaagct ctatcagatt
     6661 ttgctgaaag agtttggtcc agacatcttg tcggcagatg gacatttaga cagggtcaag
     6721 ctgggtcagg ctgtttttgc ggatagcaag ttgcgggctc gcctgtctga cctgcaggac
     6781 caaattatca ggcaggagct gttagacaga agggatgctc taaaacagac ggagcaagtt
     6841 cttttcatgg atattccttt gctttacgag gcagattata gcggggaagt aaatgaaatc
     6901 tggctagttt atgtcgatag agcacagcag ttggaacggc ttatgaaacg taatggctta
     6961 gctgttcaag atgcagaaaa tcgtctggct gctcagcttt ctttagagga aaaacggtcg
     7021 caagcccagg tggtgattga caatagcgga gcggttgagg cgaccttagc acaggttgct
     7081 cagcttttgg aggaattaaa ggatggacga cggtagtagt tattggaagc agaatttgaa
     7141 ggtggcttgg ttgggaaatt tcctaacagg gacgagtttt accttggtta tgccttttat
     7201 ctcagttttt gtggaagagt tgggggtagg tcctggacag gtagaatatt atgcggggct
     7261 tgcggtatct gttaatgccc ttgcggcagc cttgatggca cctatttggg ggagtttggc
     7321 ggaccgttac ggtcgcaagc ccatgatggt gcgagcggcc tttgccatga tttttactat
     7381 gggtggtatg gcctttgtac caaatgtttt ttggttgctt accttacgtg tcctaaacgg
     7441 ggtatttaca gggtacattc ccaatgcgac tgccttgatt gccagtcagg ttccaaagga
     7501 taagacgggg tatgctctgg ggaccttatc aacaggtgcg gtagcaggta atttgattgg
     7561 tccgacccta ggtggtatct tagcagagat gtttggcgta catatggtat ttttgctggt
     7621 gggacttctt tatgctatcg ttgtgttact gaccgttttc tatattcgag aggattttgt
     7681 tccagtcaaa aaaggcgaag agatgtcggt taaagaggta tttgagcagg tgaaagatcg
     7741 gcagatgctg gttgggcttt ttgtgacttc tatgattatt attgcagctg ctcaggctgt
     7801 tgttcctata ttgaccctct atgtgcggca tttgggacag acagataatc tgttatttgt
     7861 tgctggtttt atcatttcct tgcctggtat ggcatcgcta gtgacatccg gttatttagg
     7921 gaaaattggt gatcgtattg gcaaccatcg acttctatta attgctttga cttacagtct
     7981 gttgattaat gttttctgtg tttttgcaga aaatcctttc cagcttggtt tgttgcgatt
     8041 catgtatggt tttgggacag gagcactgtt gccttcggtc aattctcttt tgaccaagtt
     8101 gacaccaaaa gaaggaattt caaggatatt ttcctacaac caactcttta ataatctagg
     8161 ttctgttgtt ggtcccatga tggggtctgc ggtagctgct cacatgggtt atgactgggt
     8221 tttctatctg tcgagcggac ttgtcctctt taaccttatc tggtctttga ccaattttag
     8281 aaactatttg aaagtaaggg atgtttagtg agagttaaaa tcaatttaca atgttcagaa
     8341 tgtggtagta agaactactt gactagcaaa aacgccaaga ctcatccaga taagattgaa
     8401 gtccttaaat actgcccaaa agagagaaaa gtaaccctcc accttgaaac taagtaggtt
     8461 ttatggtata atgtcataag ttattgtaga aagtgaaaaa aatatgtatc aagcattatt
     8521 agcaattctg ttgattttat ctgttatttt gattgtagtg atttttattc agccagcaaa
     8581 aaaccagtct agcaacgtct ttgactcttc tagtggagcc ttatttgaac gtacaaaggc
     8641 ccgtggtttt gaagcggtga tgcaacgtat tacggctatt ctgattttcc tatggatgtt
     8701 ggatgcgctg gcacttgtta tcatttcaag taagtagata aatcagactg ggacttgttc
     8761 ttggtctatt tttttgggag tgggccagaa ctcgacaagt ataaagagtt cgtctgccca
     8821 cctccgcata agttgaatag gtatggttcg gagcgttatt cgcgaactat tgagatttgc
     8881 tttgcaaatc ctatctccca cctccaacaa tcatttgatt gttggagcaa tcaaccactg
     8941 cgtctggata taacgcaaaa tacagtcttt tagtttgctt ttagtactag gcaacgagct
     9001 gcagactgta ctgaagtacg acaaagcgag ttcaaataat gatgaaatta tttgaagttg
     9061 gaaataggga aacggagttt cctcgtacgt cgcagtaata aaagataaac taaaggactg
     9121 aaagagaaat agtgctagat tcttaactag cactctggta aatacaagat gaaaaacgaa
     9181 attataaact atattaaaga agttggtcct gtgaccatgg accagctggc agatcagttt
     9241 ggagccagtt ctgctaagag ctttacagac ttggtcaagc tggtttctag catggaaggc
     9301 agtcgtcaac ttgtctttga taatgctggg cggattactc ttcctgctcc aaaaatctct
     9361 aagaacaagg taaccttaca ggggattttc cgtgctcaca agtcaggttt tggctttgtg
     9421 accattgatg aagaggaaga tgatctcttt gtcagccgtg acgatgtcaa cttcgctatc
     9481 gagggcgacc gagtggaagt ggctatcaag aaagtggcgg acaggttcaa ggggacggct
     9541 gcggaagcgg aagtcattga tattttggaa catagcctga aaacagcggt gggcttgatt
     9601 gtctttgacg aagacaagcc agagtatgca ggctacatca agtctaaaaa ccagaaaatc
     9661 gctcagaaga tttacattaa gaagtcacct ctggtattga ccggaacgga aatcctcaaa
     9721 gttgacattg aagcctatcc aaacaagaaa cgggaccact ttgttgcgac cattcgggac
     9781 gtggtggggc ataaggatga tgtagggatc gatgtcttgg aagttttgga atccatggat
     9841 atcgtgtcgg aattccctga cgaagttttg gcggaggcca atcgggtacc agaaagtccg
     9901 tctgagaagg actttgaagg acgcttggac ctgcgagatg agatcatctt tactatcgac
     9961 ggtgcagatg ccaaggactt ggatgatgcg gtccatatca agcagctcaa gaatggcaat
    10021 ctggaactgg gtgtccatat cgctgatgtc agctactatg tgactgaggg ctcagccttg
    10081 gaccaggaag ctgtcaagcg gggaacctct gtctatgtga ccgaccgtgt ggtgccaatg
    10141 ctgccagaac gtttgtcaaa cggcatttgt tccctcaatc caaatgtgga tcgcctgacc
    10201 cagtcggcta ttatggaaat tgactgcaag ggcaaggtgg tcaagcactg gattggccaa
    10261 acggttatca aaaccacctt ccgtatgacc tattccgatg tcaatgacat gattgcaggt
    10321 aacaaggaaa agctggacaa atacaagaca atcgtgccaa gtgttgagct catggtcaaa
    10381 ctccatgaaa ccctggaaac catgcgctac aagcgtgggg ctctcaactt tgatacagct
    10441 gaagctaaaa tcatcgtcaa caaggatggt ctgccagtcg atattcaact gcgccagcga
    10501 ggcattgccg agcggatgat tgagtccttc atgctagctg ccaacgagtg cgttgccgag
    10561 cattttgcta agttggactt gccttttatc tatcggatcc acgaggagcc taaatcagac
    10621 aagttgcaga agttcatcga ctatgcgact agctttggtc tgacggtata tggtacagcc
    10681 agctctatca gccaggacgc ccttcaggat ctcatggagc gggtcaagga tgaaccctat
    10741 gcggacgtcc tcaacatgat gttgctacgt tccatgcaac aggcgcgtta ctcagagcac
    10801 aaccacggcc attacggact gggtgcggag ttctacaccc acttcaccag tcctatccgc
    10861 cgttatcccg acctgctggt tcaccgtatg gtgcgggaat atggtcacaa tccttcggaa
    10921 aaagcagagc attttgagca ggtcattcct gagcttgcca agtcttcatc cagtctagaa
    10981 cgccgtgcca tcgatgcaga gcgggaagtt gaagctatga agaaggcaga attcatgcaa
    11041 gaatttgttg ggcaagagtt tgatggtgtc gtatctagcg tagtcaagtt tggtctcttt
    11101 gttgagttgc caaacaccgt tgaaggactg atccatgtta ccaacctcaa cgagtactac
    11161 cagttccacg agcgaactct gaccttgcag ggtgagaaat caggtcgtgt cttccgtgtc
    11221 ggtcagccaa tccgtatcaa gctaacgcgg gcggacaaga tgacagggga aatcgatttt
    11281 gcccacatgc cgtccgaact ggatattgtc gaaaaagctt tgaaagccaa gcggagaact
    11341 gctagtcact ccaaccgtga ccgtgatgat cggtcagggc gaggacgggg caagcactac
    11401 aaacaagaag ctgctggtcg cgacagcgga cgtcacaagt ctggaaaaga ccacaagtca
    11461 aaaagtggca aaaaagacaa gaaaaagaaa ccattttaca aagaagtggc taaaaagaac
    11521 aagaagaaaa ggagataggc atggccaagg gtgagggcaa tgtgattgcg caaaataaaa
    11581 aagcccgcca cgactatacc attgtcgata cagtggaggc ggggatggtc ctgacgggga
    11641 cggagattaa gtctattcgt gcggggcgga tcaaccttaa ggacggcttt gcccagatca
    11701 agcagggcga ggcttggttg gtcaatgtcc atatcgctcc ctacgatgag ggcaatatct
    11761 ggaatcagga gccaacacgg acccgcaagc tcctcttgcg gaagaagcaa atcgagtctt
    11821 tggacaatga ggtcaaggga acagggatga ctctggtgcc cctcaaggtc tatatcaagg
    11881 atggctttgc caaggttttg attgggcttg ccaaagggaa gcacgactac gacaagcggg
    11941 agtcaattaa acgccgtgag caagaccgtg atatcaaacg aaccatgaag gctatcaata
    12001 gtcgatagag gctgggagaa atcccggtct tttttgttat aggaaaacaa ataccccaga
    12061 cagtttcgag actatctggg gcttattatt tatcttaatc caccgtaatc atagctttaa
    12121 tcgttttccg gttggccatg tctttgtagg cttggtcgat attgtccagg ctgtaagttt
    12181 gggtgaaaac tcgacctggg ttgatgtcgc ctttgagaac agcatcaagc aaaatttcct
    12241 tatcataggt tgtgacagaa gcagagccac caccgataat gatattttga gcaaaagttg
    12301 atccgagggc gtggttgttg taatgaggca caccaacaaa accgatgcga ccgccattat
    12361 gaaggacgcc gagggcttgg tcaatagaag cttctgttcc gacacattcg agggcagcat
    12421 cagcgccacc tccaagaatt tcacggactt tggcaattcc ttcctcgccc cgttcagcaa
    12481 caacagcagt agcccctgat tccagagcca tttgctgacg gtcagcatga cggctcatca
    12541 tgataatctg cgaagctccc cgcatttttg cagcaatgac ggcacattgc cccactgcac
    12601 catcaccgat gacaaccacc ttatcaccag gtttgacatc tgcaacacgc gtagcatggt
    12661 agcctgttgg cataacatct gccagagtca agagagattt gatcatgcct tctgagtagt
    12721 cgcttggctg accaggaatt ttgaccaagg cccagttacc gtagtggaaa cgaatatatt
    12781 gagactggta gttgttgccc cagttggtgt agcctggatg gttgtcacaa gtaccgtcaa
    12841 agccagcacg acaggcatca cagtgtccgc aaccatgtgt aaatggaacg ataacgaagt
    12901 caccaggaac aagtgttgta atgtcttcgc ctacctcttc aacgataccg atggcttcat
    12961 gaccagagtt tgtgtcaccg tggtcatggt gaatgtcttc tccgtagccc caaaggtccg
    13021 acccacagac acaggcccga acgaccttga taatgacgtc atctttggct tgaaggcttg
    13081 gtttttcgat ttctgcaacg gtcatcttac cggctttttc ataaatagct gctttcatat
    13141 tttctacaca cctacattta ttaataaatg tcctttctga ttgatatgga atattataca
    13201 ccttggagtt cactctaagt caagtgaaaa tatagatttg aaatcaagat ttctttgtga
    13261 tagactagaa acacgtttta agttttgtag acgaaaagga gagataatga ctgttttagt
    13321 tccttacaaa cgcatgccta tttggaatca ggatacggtt ccccagcatt ttttgaccaa
    13381 gcacaatacc aaggtaggta cttgggccaa aatcaaggtc ttgagaggtc aacttaaatt
    13441 tgagccgatt tcagatgata atgaaatttt gggagaattt gtctatggtc cgacagatga
    13501 tgttcccttt gtcgagccac aggcttggca tcgggtgact ttgctgacag aggatacgga
    13561 gtttttcttg gaatttttct gtcaaccaga agactatttt gccaaaaagt atgactatac
    13621 tcggacacat tcggaagtct tggaggcctt gaagagaatt gagccttgta aggtcttgga
    13681 cttgggctgt ggtcatggca ggaacagtct ctacttggcc cagcaggggt ttgaggtaac
    13741 tgctgtagat aaagatgtac caagtattcg gacactcttg caggtcaagg aagtagaaga
    13801 tttggacttg caggctggga cttacgatat taactcagct agtctggagc aggattacga
    13861 ctggattatc tcaaccgttg tctttatgtt cttggagcgg gagcgggtgc cagctattat
    13921 ccgcaatatg caggagcaga cacgacctgg tggctataac ttgattgtgg cggccatgga
    13981 tacagaagat gcgccctgtc ctatgaattt ctcctttact tttggagtag gtgagttgaa
    14041 ggaatactac cgtgactggg aactggtcaa gtacaatgaa gatttcggtc aacttcacaa
    14101 gcgggatgaa aatggccatt tcctcaagat gcggtttgcg accatgctgg ctaggaaaaa
    14161 aggataaata tttttttgaa agcacttgca aggcaggtgc ttttgcgtta caatgtaggt
    14221 gcaaatgggt aaatcatatt atactccacg aaaatcacct tctggttgga taagagactt
    14281 gtgtaaaata gtctctgagt ttatatctct ggagggcgcc tacttctgat tttcacagag
    14341 tatcaggaaa ggagaagtct atgaaaaaga cttttgttcg tatagccaca tttctaatgt
    14401 tggtttgcct attccctgtt caacttgttc aagcctgttc aggctttatt attgggaaag
    14461 ggttgacaac ggatggttct atcctttatg gtagaacgga agattatcca tatccaccaa
    14521 ataatggagc gcacaataaa aattatattg tggttccagc aacagcctat gccaaggggg
    14581 atatgttggt ggatgaatca ttcggtttca cagcaccaca cttggcaaat gagttcaagt
    14641 acacctctac gcctgatgcg gcacgtgggg atgggtcaaa cggtaatttt ggtgcgcatg
    14701 gatttaatga aaaaggtgtg tccatgactg ctactgttac agctattcca aataaaaaag
    14761 ttttagctgt tgacccattg gtaactgcgg ggggactagg tgaggccatt ttgattgact
    14821 atgttcttcc tagagtcacc agtgcgcgtg aaggtatcga attaattgcg aagacaattg
    14881 atgaaaaagg ttcggcagaa gggaatatca ttgttattgc tgataagaat gaagtttggt
    14941 acatggaaat tttgtcaggt caccaatacg tagccatcaa attcccagaa gacaagtatg
    15001 caatttttgc aaatacttac tatctaggtc atgttgattt taccgataca gaaaatgtga
    15061 ttgcttctgc aaaggttgaa gaagtagcca aacaggctga aaattatatg atggttgatg
    15121 ggaagttcca tattgccaaa tcatatggac ctgagaatta tgctgatggt gatcgttctc
    15181 ggacatacgc tggtataaaa ttgttagatc cagcatcgtc tgtaacctat gaagatgctg
    15241 tgtatgattt gctacgtcag ccaacagacc caagtcgtcg ctttagttta caagatacct
    15301 ttgccttaca acgtaaccgt tttgaacatc ttccagaatt tcgtccagac gatgaagcag
    15361 gtaaggtaaa acaaggggat aatggtgcga atgaccaagc ggcagatgcg acttataaat
    15421 acgctcttgg taatgaaaac gtcatcgatg cccatgttta tcagattaat agtagcttac
    15481 catcagcctt cggtggcaca gtatggttgg gcttggctca gacaaggaat acaccgtatg
    15541 ttccatttta tggtattgtg actgatactt atgaagcctt taaaaatcgc tcagcttctt
    15601 atgataccaa ttcatggtac tggacggtac aaaatattga taagatggcc atctcacacc
    15661 cagagttatt tggtacatca attcaagaaa aatggattgc acttgaaaaa gaatggattg
    15721 cttcacaggc agctcttgat gctcaatatg ccggactttc agaagatgca gccgtagctc
    15781 tggctccaac tgtaacagag gcaaccttgg ctcgttcagc agagattttt gcccaactaa
    15841 aagctgttga agctgaaatg atggcaaaaa ttgaagcagc aacaactccg tcatcaagta
    15901 gtactgaacc atcgaccagc actgagccat catcaagtgg taccgaaact tcaaccagta
    15961 caagtcagtc tacaagtgat tctaacacag gaggagctac ggatacttcc tcatcaagcc
    16021 gaacagtagt gccttctgat aagaaagtaa cacctaccaa taaaaaaggt aaatcaagtc
    16081 ttcctagtac aggtgagcaa gtcagcctct tgttggtggc gctaggtgtt gcaggaattt
    16141 taacagcaat ttttcttcat cgaaaaaaat ccagtaaaga ataaatagag ctagtaagaa
    16201 ataggttggg gtctctccaa tctatttttt atactcaatg aaaatcaaaa acagacttgc
    16261 tccaaaggtt tggggaacct ttggaggttg gagatagagc gaacctagtt cgtatcgata
    16321 tgatgtagat agaacactgt tccttttata tttcaaggga acaggctgaa aacctccaca
    16381 ggagcttttc agtcatcaaa tcaagtcaac aacgtctgat tttgattttc gaagagtatt
    16441 aatcaaaaat gtttacaaac gtagtcgtat attgtataat ttgtttacag atgtagtcgg
    16501 aaaaggagta acctatgaaa caagcaagct atcccattca aggtatgacc tgcgcttcct
    16561 gtgctctgac ggttgagaaa gcggttggta aactagcagg aatggaagaa gtcagtgtca
    16621 acctagcgac tgaaaaattg agtgtcagct atgatgaaaa gcttttggga ctagaggata
    16681 ttcgtcaggc agttgaaaag gcggggtacc agctagtcga taacttggtg accgagtctt
    16741 atgacatttc aggcatgacc tgtgcttcct gtgctctgac gattgaaaaa gctctgggca
    16801 aactagaggg tgtagaagaa gtcagtgtca atctagcaac agaaaaggcg actatccgtt
    16861 atagccgtca taggcaaaat ccagctagtc ttgaaagagc agttgagcag gcaggttatc
    16921 agttgattag accagaaaaa gtagaagggg ctgcggacaa gggaccgagc acggaagaaa
    16981 agctgaggca tcgatttgtc tggtcagcag catttacttt tccactgctc tatattgcta
    17041 tgggtcctat gttgccttgg ggagggcttc cgttaccagc tttgctccac caaccgctag
    17101 tctatgcgat cagccaagtt atcttgctca tccctatcct ctatatcgga cgcagttttt
    17161 tccaaaaagg ctttaaaact ctcttgcaag gccatcccaa tatggattcc ttgattgctg
    17221 ttgggacagg ggcagccttg gttcaaggtc ttttgatgat tgtgtttctt cagatgggga
    17281 aagaagtggc catgcacggt caccatccag aactgtattt tgaatcagcc gccgttatct
    17341 tgacattgat taccctagga aaatattttg aagctagagc caaaggtcag acttctgagg
    17401 cgattaagaa attgatggat ctggccccca aggcggctca ggtcttgcga aatggtcagg
    17461 aaatgcaagt gccgattgaa gaagtggtgg tcggtgacca ggttattgtt cgtcctgggc
    17521 aacagattcc tgttgatggt caggttctcg aaggccagac acgtgtggat gagtctatgc
    17581 tgacgggtga gagcctacca gtgaagaaag ctctaggcaa taatgttttt ggcggcaccc
    17641 ttaaccagca aggcgccatt accatgcagg ctaccaaggt tggtcgtgat acgactctgg
    17701 cccagattat tcgcttggta gaagaggctc aaggttctaa ggcccccatc gccaaattgg
    17761 ctgaccaagt atctgcaata tttgtaccag tcgtcatggg cttggctctc ttgtcgggtc
    17821 tggcctggta cttcttgggt caagaatcat ggattttttc actaagtatt atcatcgccg
    17881 tcttggtcat cgcctgccct tgtgctctgg gcttagcaac gccgactgcc atcatggttg
    17941 gtactggaaa aggtgcagaa aatggccttc tgttcaagtc tggtcaagct attgaaaccc
    18001 tacaaggtgt gaataccatt gtctttgaca agactgggac cattactgag ggcaaacccc
    18061 aggtgacaga catccacctc ttgtctacta aaaatcgtga gcaagttctc cagctagcag
    18121 caagtagcga acaattttcc gaacatccct tggctcaggc tctcctgcaa gcagctcaga
    18181 cagaaaagat tgaactcttg cccgccacag attttcaggc actttctggt cgtggtcttt
    18241 cggtgataat agcagagcag accatctatc tgggaaatga acggttgatg cgggaacagg
    18301 gaattgatgt ttccaaagga cgtgcagttg cggaggcatt tgcccagcaa gccaagacgc
    18361 ctgttttcct agccagccag caagaagtac tagcagtcat tgccattgct gataaaataa
    18421 aagagaccag ccgtcaggct gtccaggctc tgcaaaccat aggactagag gttgtcatgc
    18481 tgacaggaga taatgaaaaa actgccaaag ccattgccaa ggaagtcggc attgaacagg
    18541 tgatcagtca ggtcctacca gatgacaagg ctaaccaagt caagttctta caagaacagg
    18601 gcaagacagt cgctatggtg ggggatggca tcaacgatgc cccagctcta gctcaagcac
    18661 atgtgggtct agccattggt tcagggactg atattgccat tgagtctgcc gacattgtcc
    18721 tcatgcatag tgacattttg gatgtggtca aggctgtcaa actcagccag gcaaccatgc
    18781 ggaccatcaa acaaaatctc ttctgggcct ttgcttataa tgtgataggt attccaatcg
    18841 ccatgggtct gttacatgca tttggtggac ccctgctcaa tcctatgttt gcaggtgcag
    18901 ccatggccct cagctcagtg tccgtcgtct tgaacgctct gcgattgaaa acctacaaac
    18961 tatatcaaaa ataccagtca aaaaactggt atttttttac ataaacgctt acaatgtata
    19021 tattaagtgc aagggagatg tttatgacaa cctttattgg aaaaccggtg accttggtag
    19081 gtccgcaatt aagagtgggg gatgtagccc ctgattttgt cctcatggcc aatgacttga
    19141 gcttgaaaag cctgaaagac tttggcaagc agaccaaggt catcagtgtt gtgccttcta
    19201 ttgatacagg gatttgtgat acccagaccc gtcgcttcaa tcaggaactg gctgaccgtg
    19261 acaatacggt tgttattacg gtttcagcag acctcccctt tgcccaaaaa cgctggtgtg
    19321 gcgctgcagg tttggaagat gccatcaccc tgtctgatta ctatgaccat gcctttggta
    19381 aatcctacgg tgtgctcatg cgggagtgga atttgctggc gcgtgcggtc tttgtcctca
    19441 atgccgacaa tgaaatcacc tacgtagaat acttggataa tgtcaacgaa catccagact
    19501 atgatgcggc tttggaggct gttaaataaa gatataacgg tgccggaaca ggcatcgttt
    19561 tttgatggaa tatttttgtc taatttgata tactaaattt atcaaaaaca aggagggtct
    19621 tttatggaaa agacacagat tattcaagac gttttggccc tcattcaaga cttgaatcgg
    19681 gcctttcaag cagataaagt aggaacagct gatgaacaac ggttccaaca taatctgaca
    19741 gagacgacac gcatattgtc ggaggccaag tcggttacca atacagaact gattgcgatt
    19801 gagaaatttt atcgttcgac cagttttttg gttggactgg gcgacctgcg attgagtgaa
    19861 accagcaggc aggcttggcg ggcatttgac cgatattact acgaaacgat cagagaagaa
    19921 ctcaaactct atggcggctc tgctattgcc caagtgtaaa agatgaagga gagggtgaaa
    19981 accctctttt gtgctataat aagtagtaag aatacagttt ttaggaagag aagaagcgag
    20041 gtgtatcatg tcctatatcc aagtaaagaa ttcgtcaaaa tactaccaaa tgggagatgc
    20101 gaccattgtg gccaatgatg gtgtgtcctt tgagattgag cagggtgagc tggtcatcat
    20161 tctgggttca tctggtgcgg gcaaatccac tctgctcaat atcttaggtg gcatggacag
    20221 caacgacgag ggggaggttt ggattgacgg tgtggatatc gccaagctgt caagtcatga
    20281 actgaccaat tatcgtagag atgatgtagg ttttgtattt caattttata acttggttgc
    20341 caatctgacc gccaaggaaa atgtggaact ggcagcagaa atcgtcaagg atgccttgga
    20401 tgccgaggag gtcttggaac aggtcggact cggtcaccga atcaataatt tccctgctca
    20461 gctttcaggc ggtgaacaac aacgtgtggc cattgcccgt gctgttgcca aaaagcctaa
    20521 aattttgctt tgcgatgaac caactggcgc tctggactac caaacaggta agcaggtttt
    20581 acaaatccta caagatatgt cccgcaagca gggggcaacg gtgattatcg tgacccacaa
    20641 tagttcccta gcccctattg cagacagggt cattcggatg cgggatgccc gtgtgcactc
    20701 cgttgagtta cacgcagaac cacaggatat tgcaagccta gaatattagt gggtgaaact
    20761 atgaaaaaga aaatctactg gaaggatatg agacaatctc tgctttcttc caaggggcgt
    20821 tttttgtcca tatttagcct gatgatgcta ggagctctag ctttgacagg tctgaaagta
    20881 acagctccca atatggaaaa gacggcccaa gcctacatag ccagtcatcg aacgatggat
    20941 ttggcagtaa tttcaggtct gggacttagt caagctgacc ttgacgaatt agaaacgata
    21001 gaaggtgcta ctcttgaagc gggctacttt aaagatgtgg ttactaatga aggtcagact
    21061 gcaattcggc tgttttcagc tcccaagacc ctatcgacct ataaactagt agaaggggaa
    21121 gtgcccagcc aaaaggggca aatagcccta tcagcttctt tcaaaaatcg ttacaaattg
    21181 gacgatactg ttcaagtgac ggagtctgat aaggatggaa aaatcttgac acagacaagt
    21241 tttactcttg tgggctttgt cgcatcagca gaaatttggg acaatgaaac catgggcatg
    21301 gctgtaagtg gtgatgggca gttaggtggc tatggccttg ttagtcagga taccttcaaa
    21361 tcagaagttt acactattgc ccgtattcgc tatgatgatt tggttgattt accatattac
    21421 agtcaggcct atcaagacag gttggaccag caccaagaag acttagaaaa gttattggct
    21481 aacaatgatg aacaacgatt ggcaactatt cagtcagatg gacagacaga tatttcaaaa
    21541 ggggaagagg aaattgctca ggctcagtcc cagcttgaac aggcagagtc agaactggaa
    21601 acaggggagg agcaattagc gacaggtcgc agtgagtttg cgcgtggtag ggctaaggtt
    21661 gtccaaacgg aagctgaatt acgagctgcc caagcccaac tattaaaaaa taagtttgaa
    21721 ttggaagaaa gtaaaaagga attggacggg cgtaaggctc aattagaaca gactgcttca
    21781 tttctagacg gctatcagat ggaattagaa atgtcagccc agcagttaga agaggccaag
    21841 caagaattgg attcccaaaa tgcccaactg agtcagaccg catctcagat ttccacaggg
    21901 cgagctagtt ggtttcaagc tcagcaagag ttagaccttg aaattgccaa tcacttgcaa
    21961 gaaggtcaaa ccctctcaga ctatcctgac ttgttggcca gacaggaagg tttggatgtt
    22021 gagaaaagcc gattagacca aatggaatct gcacatgagc aggccaacca agcctattta
    22081 caaggttatg attactatca aaccaaacaa aatgaataca atagcaatct agcccagtac
    22141 caaacttgga atcaagaata tcaggcaggt ttggcccgtt atcaagcagg tttgagtcag
    22201 tatgaacaag gcattgcagc ctacaataaa ggagtagaag actatgaatg gggactgagt
    22261 cagttagagt cttcaaatca actgctccgt caggaagaac tccgtttgga ggaggctgat
    22321 aaagagttaa gtcaagctca atcccaattt tctgataaga aagctgtggc cgatcaagaa
    22381 attagccaag ctcaaaccga gattgctcag gctaaatccg acttaagtaa gctggcaaaa
    22441 gcgccctatc aggtctatac tcgttcaagc ctaccaggtg gagatggcta tactacctat
    22501 agcaatgcga ctaggtctat tgctgcagtg ggcaatgtct tcccagttgt tctctacctt
    22561 gtcgcagctc tagtgacttt taccaccatg gcccgttttg tcgatgaaga gcgaacacag
    22621 tcaggtatcc tcaaggccct tggttatacg aatagacaga ttatggctaa gttcattctc
    22681 tatggtttgg cggcaggtct ggtaggaaca atagtcggca taatagcagg taatctcctc
    22741 ttgtctccac ttatttccga cattattacg cagacaactg tgattggacc agctaaactt
    22801 catttttatc ccctttggac aggtttagcc ttgctcctat ctttggtttc ctcggtcctg
    22861 ccagcttact tggtggctcg tcgggagctg acagaaaaac ctgctcagct acttttacct
    22921 aagccaccgg tcacaggctc cagcatttgg cttgaaaaat ggccggctat ctggtctcgt
    22981 ctgagcttta cccataaggt gacagcccgc aatatcttcc gctacaagct tcgtatgctt
    23041 atgaccatct ttggagtagc agggaccgtc gcccttcttt ttggaggact gggtatccgt
    23101 tcctccatct caggagttgt ccaacgacag tttggggagc tgatccatta tgatatgttg
    23161 gtggtggaaa atagcagagc tactgaggaa gagttagata agttaacaca ttttcttcag
    23221 tcagatcagg ttcgccaatc cttgccagtg gcttttgaac agctcaatca aacggtggaa
    23281 gaaaacggtc agagaaagaa tttatcgatt agtctctaca tttcagaccg tcgggatttt
    23341 ggaaatcaag tcagtctgga aagctcgaca ggtcagccta ttaaactgag tgacaggggg
    23401 attgtcctca cagaaaaatt ggcgcaaatt tacggggtct cagttggtga taagttatca
    23461 ctgaccttgg aagataagga agtttcagtc agggtagagg ctgtggctga tatgtacgca
    23521 gggcatttta tctacatgac agatagctac tacgaacaag tgaccggcaa gcagaagact
    23581 gccaatgcct atttggttca attgaaagac agtcagttgg ggcatattca gactcttgca
    23641 agtcaactcc tagcgatgcc agcagtcaga agtttggttc aaaatacttc tcttatagac
    23701 atgcttacca cgattgcagg ctcgcttcag accatcatga ccattctagt catcctatcc
    23761 atcttgctcg gcttggttat tctttacaac cttacaatta tcaatatgtc cgaacggatt
    23821 cgtgaattat ccaccatcaa ggtgctgggc ttccacaata gagaggtgac catgtacatc
    23881 tatcgtgaaa ccattgccct ttcgctgatt ggtatgttgg ttggactggt tggcggtatt
    23941 tatctccaca agctccttct tgccatgatt ggttcggaca gtatccgctt caatccatct
    24001 gtcggactag aagtctatct cattcctatc ttagccatta gtggaatttt gtctgttctt
    24061 ggttggtatg tcaatcacca tctgagaaag gttgatatgc tggtggcctt gaagtcggtt
    24121 gattaaaaca gtctgggaat agactgtttt aacccgagcc taaaaacacg aaagcggata
    24181 gtctgtattg tgaactaatt tgaaatatga gaaacacagc tataaagcaa aaaaatccta
    24241 gtgcatctag gatttttctg taaacacaag tctagaagtg tgctataatc atctcatgaa
    24301 aaaaataagt gatttaggat taacaggtcg aaaattagtt ggagaaggtc taatcttagt
    24361 cttcatcggt attggatttt taattgctgg ttggcaattt ccaggactga ttcttcgatt
    24421 tgttcacgca ggtctattct ttttagcttt atatgaattg agcatgacat ttttcagaaa
    24481 gaagaaatca agtgaaagtg ttattgcgct agttgggaag gctgttcttt tcgggatcct
    24541 agccagtctc gatttagctg ttcaaattcc cctctatttc gcggctattt ttgttgggat
    24601 ttatcaactt tttacagctc ttatcaattt cattactttt tacctatatc gaaaagatgg
    24661 ggtacagcca cggattcgct atttaataga tggaatttgg ttattcttac taggttcagc
    24721 ttccctattt gtgtcaggca cacagctggt tgttcaaaca attgtcatcg gaggttatct
    24781 gatcttgtat ggactaacta atctgcgtga tggtttcttg tttgaggagg cgattgagca
    24841 acaaaatctt aagcgccatg tccgtttgcc tctaccttta tttttagcag ctttgattcc
    24901 tcgtatgacc ctgcaaaaag tcaatgatta cttggcggat aatgagggac aaacggctca
    24961 atctatctat aaccgccaca aagaaattgc ggagcttcca gcattagaag tctttgtgca
    25021 tgtaggtgaa gagggttttg gggctgttgg acatgttgat ttgagctata agggacgagt
    25081 gtatggttat ggttcatacg atgtcctgtc tgagcgtttg ggtggtgcga ttggtgatgg
    25141 agtcttgttc aaagcagagc gccaagccta tattgacttc tgtaatcaag aaggaatgac
    25201 catgttaggt taccagctga gcttaagacc agagcaagaa gaggcaatag aaactcgatt
    25261 ggctgaaatt gataatctgc ttcttccttg gaatccaagt cctgaaaaag tatcgaaaac
    25321 ggtagatggg cagccgattg aaatgtatgc ctatcgaatg aaagaggcaa tacatgcgga
    25381 attattcaaa tttagaaagt ctaaatttaa aacctatttc gtcctatcta ctaattgcgt
    25441 tcttctggct gattctgtga ttggacaagc tggaaccgat atacttggta tgcatggatt
    25501 tatcgcaccc ggaacctacc aatcttatct ggatcaagaa tttgaaaagc cacatagttt
    25561 agtagtcgca aagaacattt attatcgaaa agaaaaatcc tagtcactga ctgggatttt
    25621 ctgttattcc agctctctaa gttccacaaa actcccccat ccatgctata atagagacat
    25681 gaaaaaaaat aaattattat taatcgatgg ctcttctgta gccttccgtg ccttctttgc
    25741 actctataac caaatcgacc gcttcaagaa tgccaacggt ctgcatacca acgccattta
    25801 cggcttcaat ctcatgctgg accacatgat gaagcggatt gagccgaccc acattcttgt
    25861 ggcttttgat gctggaaaga ccactttccg tacggagatg tatgcagact ataaggcagg
    25921 tcgtgccaag actccagacg aattccgtga gcagtttcct tttatccgtc agatgttgga
    25981 tgccatggga gtcaagcatt acgaactgga tcagtatgag gcagacgata ttatcggtac
    26041 cttggacaaa atggcagagc ggacagacat ccctttcgat gtgaccattg ttagcggtga
    26101 taaggatttg atccagctga ctgatgagaa caccgtagtc gaaatttcca agaaaggcgt
    26161 tgccgaattc gaagaattta ccccagccta cctcatggaa aaaatgggga ttagccctac
    26221 tcagtttatt gacctcaagg ctttgatggg agataagtcc gataacattc ctggcgtgac
    26281 caaaatcggt gaaaaaacag gtctaaaact cctgacagaa tttggttctt tggatggtat
    26341 ctacgaaaac attgacagca tgaaggcttc taagatgaaa gaaaacctga ttgctgataa
    26401 ggagaaggcc ttcctatctc gtacacttgc taccattgat actcaggcac caatcgaaat
    26461 tggtctggat gacattgttt atcaaggccc caaggtggat gaactgggac aattttatga
    26521 tgacatgggc ttcaaacaat taagggctca gttaggtacg accagctcgc aagaggaagc
    26581 ggtgctcgat ttccaaatcg taacggaaat cagccccgct atgctcaaac aagaccagtt
    26641 tttctatttt gaaatcttgg gtgaaaatta tcaccgagag gatttggttg gtttagcttg
    26701 gggagataag gagaaaatct atgtcggagg gcctgagcta cttgatagtc cagtcttgcg
    26761 tgacttttta gaaagccaaa ccataaaaac ctatgacttt aagcgtggaa aagttcttct
    26821 cgaccgaaaa gagattaagc tacctccagc tactttcgat agccgtctag ccaaatactt
    26881 gctttccacc gttgaggaca attccctaac aaccattgcc aacctctatg gtcagaccag
    26941 tcttgttcca gatgaggcag tttatggcaa gggtgctaag ttagacttgc cagaacgcga
    27001 gatctttttc ccacacttgg cacgtaaggt tcaggttttg attgagacag aagagccgat
    27061 gttgaccaaa ttagaagaaa atcagcaatt agatcttttg tttgacatgg agttgccact
    27121 agcaaatgtt ttagctaaga tggaaattgc tggtattaaa gtggaagctg agactctcaa
    27181 agctatgcaa tctgaaaacg aagttttgat tgaccagttg accaaggaaa tctatgaact
    27241 agctggtcag gaatttaata tcaattcgcc taagcagctt gggaccattc tctttgaaga
    27301 tatgggacta ccattggaat ataccaagaa aaccaagaca ggctattcaa cagcagtaga
    27361 tgtcttagaa cgattagcgc caattgcacc agttgtttca aaaatcttgg aataccgtca
    27421 gattaccaag cttcaatcaa cttatgtagt cggcttgcag gatgccattt tagaagatgg
    27481 taagatccat acccgctatg tacaggattt gacacaaaca ggtcgtctat cttcaacgga
    27541 ccctaacttg caaaatattc ctgttcgtct agaacaaggg cgcttgattc gtaaggcatt
    27601 tgtgccatcg cttgaaaata gtgtcctttt ggcatcggac tattcacaga ttgaattgcg
    27661 cgtcttggct catatttctc aggaccagca tttgattgag gccttccagc aaggtgcaga
    27721 tattcacact tcgacagcta tgcgggtctt cggtatcgaa aaagctgagt atgtgacacc
    27781 aaatgaccgt cggaatgcca aggctgtaaa cttcggtgtc gtctatggta tttcagattt
    27841 cggtttatct aataacttgg ggattacacg taaggaagct aaagcctata tcgataccta
    27901 ttttgaacgc ttcccaggaa ttaagaatta catggaaacc attgttcgtg aggcacgtga
    27961 taaggggtat gtagagacga tttacaaacg ccgtcgtgaa ttaccagata taaactctcg
    28021 caatttcaat gtccgtaatt ttgcggaacg aacagccatc aactcaccaa ttcaaggttc
    28081 ggccgcagat attctcaaag tagccatgat taatctcgac aaggctttga ctgaagcagg
    28141 tcttgcgaca cgtatgttat tgcaagtaca cgatgagatt gttttggaag tcccagtggc
    28201 agagctagaa acggtaaaag ccatggtaaa agagactatg gaatcggcga ttagcctgtc
    28261 tgtaccattg attgcggatg aaaatgaagg ttcaacatgg tatgaagcaa agtaagaaag
    28321 gaaaaagtat gaaaaaatta attctttgta ttggtctatg tagtcttcta cttgcaggtt
    28381 gcggaaaagc atcgcagaag actgagaaaa caccagcttc ttcaggggaa gagttttcaa
    28441 ccaccttacc gattttgcaa gaaaaacagg atactacgaa cgaacagttc aatgttttag
    28501 caaatgcaga ggttgtagct gttaatagta caccacctgc cgacgaaaca caaaaaggcc
    28561 acaagattta tgcggccaat aatggtgttg ctgaaaaaga tgaaaatggg aatatcaagg
    28621 aatatttccg ttattatgat gtgccatcta cttggacaat taatgcagaa aatactaagg
    28681 atgagacggt cgctgcagtc tacgatgtta aagagggggg aagcaattac atggttcaaa
    28741 tctataatat caatgctttt aatcagtctc cattggaaga cggtcgcaat atgacgccgg
    28801 aggaattaga agcacggatg gttgaaacaa atcattcttt cagtgaaaag acggttgtca
    28861 caatcaatgg tcaggaatgg caggttgggc gacagttatt aacagatcaa aaattagctc
    28921 gtctgacttt ttatcggatg gaatcaacag ctgcttatga tgactccgtg gttgtgggtt
    28981 caatctatta ttccttagat ccaggagatg ataaaaagcg taccaatctt aaaaaggtta
    29041 tcggccaagt taaggatgtt gtgtaccaaa ttgctaaaaa ataatcaagg tagacaagct
    29101 ataatcagta acgagttaaa actgacaaga ttgtgttata gcacacctta ttcattaagc
    29161 ttttattgat tctaacattt aatatacatt aaaggaggtc tctatgtctt attccttcca
    29221 aaatcctagt caggacatta tctttgacta tctgaaaaat gctaagacca ttgcagtggt
    29281 cggtttgtct agtcgagaag agacggcagc ctaccgcgtt tccaagctca tgcaggaagc
    29341 gggctataaa attattccag ttaatcccaa ggctgctggt gggactattt taggcgagtt
    29401 ggtctatagt agtctagcag agattgacca acctattgat attgtagatg ttttccgcag
    29461 aagtgaattt ttaccagagg tagcacagga atttatccag tcgaacgcca aagttttctg
    29521 ggctcaactc ggtttagaaa gccaagaagc agaaaaacaa ttgcgtcagg caggtcgtaa
    29581 cgatattgtc atgaataaat gtatcaagat tgaatatctt gaaatgaaag aacaatacta
    29641 ataagtcggg tatatttatc cgactttttt catcgaaaaa gcagctcaat gaaccattga
    29701 actgccggat gactctaagg aggagcactt agaattggaa taaattacta cacatggcgt
    29761 aaaaaataga aaggaagaaa tctgctccaa taccaagcag gctaaaatag tcctttgtcg
    29821 c
//
LOCUS       DBNFCK010000010        27461 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.010, whole genome shotgun sequence.
ACCESSION   DBNFCK010000010 DBNFCK010000000
VERSION     DBNFCK010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 27461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27461
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.010"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            1..135
                     /locus_tag="AC1PIW_000362"
                     /pseudo
     CDS             1..135
                     /locus_tag="AC1PIW_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
     gene            153..3887
                     /locus_tag="AC1PIW_000363"
                     /pseudo
     CDS             153..3887
                     /locus_tag="AC1PIW_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167702.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0018262 - isopeptide cross-linking
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SspB-related isopeptide-forming adhesin"
     gene            3888..4079
                     /locus_tag="AC1PIW_000364"
     CDS             3888..4079
                     /locus_tag="AC1PIW_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HIP6605330.1"
                     /translation="MNPKSIYEKDSDQDGLTDAQELAFGTNPQSVDTDGDGQADLEEL
                     QSGHSPLVPQKELCDGLEL"
     gene            4063..4614
                     /locus_tag="AC1PIW_000365"
     CDS             4063..4614
                     /locus_tag="AC1PIW_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605331.1"
                     /translation="MAWNFDTMKEALSEMEKVDYQEFIKAFLSLELSISDRTILNQVY
                     QDYMDEDDLSLISDELRVKVDSYQDEVQADMTDILEKLYRTGEGSSFIMDLMSSNSLS
                     DTLEQYEVLDSDDYSPLSLETLQAMIQQDLAISSQDYFGDLVHLALQKDLLDQKSHFL
                     QHYVATVMEGIPQERDQRALVLD"
     gene            4663..10611
                     /locus_tag="AC1PIW_000366"
     CDS             4663..10611
                     /locus_tag="AC1PIW_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HIP6605332.1"
                     /translation="MNQEVLLQMMRATIPRDRALLEAFLYYQAEHFDEEWDSLIRQFL
                     SNRQANISPVQVLHFETDVSAFVQASPYDNAHDLLTYTQVFGQTGLQKLDKLSPSEKD
                     LVIEVALFNLATRFQLLDSNGHYQTISPDSLLQKSRGANLVNVYRVANNLADRISRDI
                     EQFLLTYEPELETRADKTVLENEETGDEHKVSSNQAIPFREDGSLIIASLDVDLSQLN
                     VQTGKTSHLPAYEELLLQRKFEILTYFDHIRNERSKVPSFKRGDFDTEMEMTPIFEGK
                     ELLTYLEADGSPYELKRTLTTVEEKELEKIGQAIRIENQEKLTQLGIELSQFDPDRVG
                     ILLDAAGRYRLKNADLALLGGYPKASVTQLALATELLQMGLSHEKVEFFFGSQLSLEE
                     LRQVAYAFLHEELSREDAEQFEKDKGNQPDLTLRDWKSKLEKAERKEVVDEEFVENPL
                     VQRVLDTYPLGSLVSYKGQDFEVVSVSDARLNGLIRIELVNDFSDIIEQNPVLYVRTW
                     EEVSQALHQPKAEPQTELEEADQELNLFSFLEEEPVQSIGLLEPDGSEKGHNDTDLEE
                     TDNQIPEEEVVETIPEIPVTDFYFPEDMTDFYPKTARDKVETNIAAIRLVKTLETGRR
                     QASTSEQELLAKYVGWGGLANDFFDDYNPKFSKEREELKSIVTDKEYSDMKQSSLTAY
                     YTDPALIRQMWDKLERDGFTGGKILDPSMGTGNFFAAMPKHLREKSELYGVELDTITG
                     AIAKHLHPNSHIEIKGFETVAFNDNSFDLVISNVPFANIRIADNRYDKPYMIHDYFVK
                     KSLDLVHDGGQVAIISSTGTMDKRTENILQDIRETTEFLGGVRLPDSAFKAIAGTSVT
                     TDMLFFQKHLNKEYVADDSAFSGSIRYDKDSRIWLNLYFDGEYNSQVLGTYEVRNFNG
                     GTLSVKGTSDDLIASVEAALHQVKAPREIDRNEVIINPDVLIKQVIDTSIPAEMRENL
                     GQYSFGYQGSTVYYRDNKGIRVGTKTEEISYYVDEEGNFKAWDTKHSQKQIDRFNALE
                     VTDNTALDVYVTDDAAKRGQFKGYYKKTVFYEAPLSDKEVARIKGMVDIRNAYQEVIA
                     IQRYYDYDKKTFNHLLGKLNRSYDSFVKRYGYLNSAVNRNLFDSDDKYSLLASLEDES
                     PVPSGNSVIYTKSLAFEKALVRPKKEVKKVHTALDALNSSLADGRGVDFDYMMSIYQV
                     ESKMNLIEELGDLIMPDPEKYLNGELTYVSRLDFLSGDVVTKLEVVDLFVKQDNQDFN
                     WSHYAGLLEAIKPARITLADIDYRIGSRWIPLSVYGKFAQETFMGNAYELSDQEVATV
                     LEVSPIDGVITYQSKFAYTYSNAMDRSLGVPASRYDSGRKIFENLLNSNQPTITKQIV
                     EGDKKKNVTDVEKTTVLRAKETHLQEFFQDFVARYPEVQQMIEDTYNSLYNRTVSKTY
                     DGSHLTIDGLAQNISLRPHQKNAIQRIVEEKRALLAHEVGSGKTLTMLGAGFKLKELG
                     MVHKPLYVVPSSLTAQFGQEIMKFFPTKKVYVTTKKDFAKAKRKQFVSRIITGDYDAI
                     VIGDSQFEKIPMSREKQVTYINDKLEQLREIKLGSDSDYTVKEAERSIKGLEHQLEEL
                     QKLERDTFIEFENLGIDFLFVDEAHHFKNIRPITGLGNVAGITNTTSKKNVDMEMKVR
                     QVQAEHGDRNVVFATGTPVSNSISELFTMMNYIQPDVLERYQVSNFDSWVGAFGNIEN
                     SMELAPTGDKYQPKKRFKKFVNLPELMRIYKETADIQTSDMLDLPVPEAKIIAVESEL
                     TQAQKYYLEELVERSDAIKSGSVAPSRDNMLKITGEARKLAIDMRLIDPAYTLSDNQK
                     ILQVVDNVERIYREGENEKATQMIFSDIGTPKSKEEGFDVYNELKDLLVDRGIPKEAI
                     AFVHDANTDDKKNSLSRKVNSGEVRILMASTEKGGTGLNVQSRMKAVHHLDVPWRPSD
                     VGRILRTFKIKKNVEVTDNGKDNF"
     gene            10592..10966
                     /locus_tag="AC1PIW_000367"
     CDS             10592..10966
                     /locus_tag="AC1PIW_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HIP6605333.1"
                     /translation="MAKTIFEEMGGKYERQGDYLIPCLTVPAEEEQPIGIWGQRHLDY
                     LKHHCKVTYTNLLTSGRLNAYLADIDRQAQERFERLIEGMKQAQGITEQLKAENALEW
                     TGCLNNIRACAREIVEKEIIFA"
     gene            11333..13252
                     /gene="tet(O)"
                     /locus_tag="AC1PIW_000368"
     CDS             11333..13252
                     /gene="tet(O)"
                     /locus_tag="AC1PIW_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(O)"
                     /protein_id="HIP6605334.1"
                     /translation="MKIINLGILAHVDAGKTTLTESLLYTSGAIAEPGSVDKGTTRTD
                     TMNLERQRGITIQTAVTSFQWEDVKVNIIDTPGHMDFLAEVYRSLSVLDGAVLLVSAK
                     DGIQAQTRILFHALQTMKIPTIFFINKIDQEGIDLPMVYQEMKAKLSSEIIVKQKVGQ
                     HPHINVTDNDDMEQWDAVIMGNDELLEKYMSGKPFKMSELEQEENRRFQNGTLFPVYH
                     GSAKNNLGIRQLIEVIASKFYSSTPEGQSELCGQVFKIEYSEKRRRFVYVRIYSGTLH
                     LRDVIKISEKEKIKITEMCVPTNGELYSSDTACSGDIVILPNDVLQLNSILGNEMLLP
                     QRKFIENPLPMLQTTIAVKKSEQREILLGALTEISDGDPLLKYYVDTTTHEIILSFLG
                     NVQMEVICAILEEKYHVEAEIKEPTVIYMERPLRKAEYTIHIEVPPNPFWASVGLSIE
                     PLPIGSGVQYESRVSLGYLNQSFQNAVMEGVLYGCEQGLYGWKVTDCKICFEYGLYYS
                     PVSTPADFRLLSPIVLEQALKKAGTELLEPYLHFEIYAPQEYLSRAYHDAPRYCADIV
                     STQVKNDEVILKGEIPARCIQEYRNDLTYFTNGQGVCLTELKGYQPAIGKFICQPRRP
                     NSRIDKVRHMFHKLA"
     gene            13307..13477
                     /locus_tag="AC1PIW_000369"
     CDS             13307..13477
                     /locus_tag="AC1PIW_000369"
                     /inference="COORDINATES: protein motif:HMM:NF025568.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HIP6605335.1"
                     /translation="MKCEWILCPVCGSKTHNKIRKDTVLENYPLYCPKCRQESLIKVD
                     NLKITVIKEPDA"
     gene            13823..14476
                     /locus_tag="AC1PIW_000370"
     CDS             13823..14476
                     /locus_tag="AC1PIW_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIP6605336.1"
                     /translation="MKVTEPLRFFFCRFLLESAAGLFLVCVAAPRLSIWIFTFQKFRL
                     EVLAVKYAPRKVYIKESGGYVELSYTDFCRRRQADKGYMDKLFIPVQGCLLEVVREQY
                     ADFYRDRERWRYLQKLDARNSLLSLDGFTDSEGNPLDFIADEAADIAETVVNAVMVDR
                     LKAALPLLSDSEQELIQAIFFDGLSEREVGARLGITQSVVNKRKARILRKLRKIIEN"
     gene            14571..14996
                     /locus_tag="AC1PIW_000371"
     CDS             14571..14996
                     /locus_tag="AC1PIW_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIP6605337.1"
                     /translation="MAYNHGREDRKWRIWKEAEEKLLRECGVDEVTIEQIRIADRADF
                     NSNRRFYRWTNDVAEYLEDMADRERQAEVNTVAELLDEIESENLYQVLVTVDGRTLKI
                     VLLKMQGYSTKEIAPLVHLTTGAIYARLDHLRKKLRKIL"
     gene            15080..15553
                     /locus_tag="AC1PIW_000372"
     CDS             15080..15553
                     /locus_tag="AC1PIW_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HIP6605338.1"
                     /translation="MAYKAKAYTLREESTESGIRYFISFKDGQGEHHELEVSEQLFFE
                     FRQMERRNRNLLQWDERHREFSEVWDETLNRRALKLPKSIEEQMIEAERAELLCKAVD
                     RLPEIQRRRFLLYYEYEFNFYQIAAMEHCTASAIQKSVAVAKKKVKAEMRKYLQP"
     gene            15707..16731
                     /locus_tag="AC1PIW_000373"
                     /pseudo
     CDS             15707..16731
                     /locus_tag="AC1PIW_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521636.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            16984..17250
                     /locus_tag="AC1PIW_000374"
     CDS             16984..17250
                     /locus_tag="AC1PIW_000374"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605339.1"
                     /translation="MPDVPSGLPYGRSYALLSYALRLRIKERRHDGYSSCEGVSQKHR
                     HHRHDRHAGDKGDSYGNRQGASQNCRHHRHRCHPKGSYPPKKGR"
     gene            17257..18222
                     /gene="mobV"
                     /locus_tag="AC1PIW_000375"
     CDS             17257..18222
                     /gene="mobV"
                     /locus_tag="AC1PIW_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="HIP6605340.1"
                     /translation="MPYAILRFQKRKAGGVAACERHNERKKEAYKSNPDIDMERSKNN
                     YHLVAPPKYTYKKEINRKVAEAGCRTRKDSVMMVETLITASPEFMNQLPPEEQKAYFT
                     MALDFISERVGEQNILSAVVHMDERTPHMHLCFVPITPDNKLSAKTILGNQKSLSEWQ
                     TAYHERMSSRWNQLERGQSSMETKRKHVPTWLYKLGGRLDKQYGEIVSALSDINAFNA
                     GKKRDKALELVAAWLPEVEKFSKEIGRQQAYIDSLKEQIGQEADYAGRMRDEKYEQEL
                     KVQKANQRIFELQRTNEQMGRLLSKIPPEVLEELQRTGRNKSRER"
     gene            18230..19084
                     /locus_tag="AC1PIW_000376"
     CDS             18230..19084
                     /locus_tag="AC1PIW_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIP6605341.1"
                     /translation="MKKQDFKVLKTKDLYPFPDNPFHVVEDETLSELAESIKEFGIVT
                     PIITRPKEDGNGYEVIAGQRRVRASELAGINTVPAFVLPLDRDRAIITLVDSNLQREN
                     ILPSERAFAYRMKSEAMKRQGFRTDLTSSQVVTKLRTDDKVAQGFGVGRMTVQRFIRL
                     TELIPPILQMVDEGKIALTPAVELSFLKKDEQENLFATMESEEATPSLSQAQRMKSMS
                     QSGQLDMDMIFSIMTEEKGNQKETLKINTSKLKKYFPKDTTPKQMEETIIRLLERELQ
                     RKRSRDSR"
     gene            19161..19391
                     /locus_tag="AC1PIW_000377"
     CDS             19161..19391
                     /locus_tag="AC1PIW_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HIP6605342.1"
                     /translation="MKEIQYEIVKEIAVLSASDSGYTKEINLISWNGREPKYDIRSFS
                     PNREKCGKGITLNADEAAALLEALQKEVNSGD"
     gene            19462..19653
                     /locus_tag="AC1PIW_000378"
     CDS             19462..19653
                     /locus_tag="AC1PIW_000378"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605343.1"
                     /translation="MGEDRKTTGRRKRIVPKGQVQMIISREYIGTQTVAEAFVPIISE
                     DIRKKIAEGDTFDNEGLSA"
     gene            19707..21590
                     /locus_tag="AC1PIW_000379"
     CDS             19707..21590
                     /locus_tag="AC1PIW_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIP6605344.1"
                     /translation="MAGIRTENKIYEVGIYCRLSKDDGTDNESASIATQKSILTDYVK
                     RQGWHLAKTYVDDGYSGTNFQRPSFQNMIKDIENGLINCVITKDLSRLGRNYLDCGLY
                     LEVFFPEHNVRYIAVNDGVDTLNKSAMDITPFRNILNEMYSADVSVKIKSAYRARFQQ
                     GKFMGTTAPYGYVKDPADHNHLLIDDKVAHVVREIFDLALAGNGIAKIRKHINKQHIL
                     RPAAYAVEQGATGYERYFEDNEENRYIWSENSVRGILRSPIYAGNLAGYKRIAANMKS
                     KKRPSKLPEEWEVIPDTHEGIVTQEEFDTVQQLMTSRRREQNAGGFENIFSGVIKCAD
                     CGYAMRAASANRRKRPDIIDCVQYTCNNYGRYGNVMCTAHSIEARDLFNAVLADINRF
                     ADMAVNDEKAVRAIERRLTETDQSRAKSLEKEKKKLNKRLAELDRLFSSLYEDKVMER
                     ITERNFEMMSGKYQKEQLEIEARLKEVTETLNDSYEKSQGVRDFLSLIRNYQGLKELD
                     ATIINALIDKILVSEREKLADGTVRQEIKIYYKFIGFVGELHITPTKRWTALKPKNCT
                     VCGVEYVPSSGISKYCPACAKRIQREKSNESKRRSRERNRRACIELSAKNDRLTLSSE
                     MDD"
     gene            21557..22516
                     /locus_tag="AC1PIW_000380"
                     /pseudo
     CDS             21557..22516
                     /locus_tag="AC1PIW_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071192.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            22587..22886
                     /locus_tag="AC1PIW_000381"
     CDS             22587..22886
                     /locus_tag="AC1PIW_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5962 family protein"
                     /protein_id="HIP6605345.1"
                     /translation="MGQTVEEMRYQLEEWLSQGFTSSEDRANYQTLKEQYEDETFDYS
                     FSRREITGQLEIIITSRENDFPSLDKVTKAEYLDLVAQLDDLDKRQADYYRKQLA"
     gene            22900..23190
                     /locus_tag="AC1PIW_000382"
     CDS             22900..23190
                     /locus_tag="AC1PIW_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5966 family protein"
                     /protein_id="HIP6605346.1"
                     /translation="MLEQILQSLLIIAAIGLMLFVLYRIVKVSGALFLIGLISGLVFI
                     EIYGIYLFFTERHLYSEDLAANGIWSFTGFYIALNLFLAISLSMRWWRNRIV"
     gene            23285..23923
                     /locus_tag="AC1PIW_000383"
     CDS             23285..23923
                     /locus_tag="AC1PIW_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIP6605347.1"
                     /translation="MLNKVKARFLIGLGGLIAVSFMVMIGYTIGSQTISKQTENQIRA
                     EANKLVSKKKQEERATVLSDELVNEFLTQYFTKVQLGENNARIKPYMTDSAFSEEEAN
                     QNKAINQVYKDYMLDYRFESASIYVNTESNVALAEVTYQVTYVSDLSEQQQRTSQTET
                     KTVMLSYSKVSDKLLVNQLTIWNGKLEDMKEATDGANSSIPTIQGTTTSENN"
     gene            23963..25063
                     /locus_tag="AC1PIW_000384"
     CDS             23963..25063
                     /locus_tag="AC1PIW_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HIP6605348.1"
                     /translation="MTRIKAVKQKAILDVAESLGYSFRRLSGHIYEHPDHDSFRIFAD
                     TNTFKWFSRDIQGDVIDFVQLVAGVTFKEAVSYLETGDFEQAKLIEETYQPFQYYLHE
                     EPFQQARTYLQVVRGLSDQTINTFGRQGLLAHATYQSEPVLVLKSYDHNGTLQAASLQ
                     GLVKNEEKHDRGYLKKIMKGSHGYVGISFDIGNPKRLIFCESVIDMMSYYQLYQKQLS
                     DVRLISMEGLKLSVIAYQTLRLAAEEQGKLAFLDTVNPSRLSHYLQAIQETTTFFQTH
                     SNALTLAVDNDEAGREFCQKLSDKGLPLSQDLPPLQGLETKLDWNDIVKRQKELSLRD
                     LIQSAQTKVIRDHPPPKREHTFDVITKSSPLS"
     gene            25091..25318
                     /locus_tag="AC1PIW_000385"
     CDS             25091..25318
                     /locus_tag="AC1PIW_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5965 family protein"
                     /protein_id="HIP6605349.1"
                     /translation="MSVIERLAEKVARQEEKVSRETEKLEHYRDQLQTAMYSTFIKRQ
                     QSSQLSFHEALEQAFGKETTLHPNYRNEDTE"
     gene            25315..25704
                     /locus_tag="AC1PIW_000386"
     CDS             25315..25704
                     /locus_tag="AC1PIW_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5945 family protein"
                     /protein_id="HIP6605350.1"
                     /translation="MSKTWNFDQPLDDVKPTSSHEERAKIAALFHKQDEKPIEEVDYV
                     AAFEQQQKESESKDVQTHESKVKQSQPKKVNITSDYKQHLADTIAQNNKDISACQKQI
                     EELHQLIDEKKIQNKKLQAISVAIDDL"
     gene            25754..26044
                     /locus_tag="AC1PIW_000387"
     CDS             25754..26044
                     /locus_tag="AC1PIW_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605351.1"
                     /translation="MKFFQKKKQNQDQFKRLIHRLSEMSDSELTKVEQLLDVVFDSSF
                     KSSERVEISRDIITDEEQILDKSIQEAKNKLNTEQLEKRIEEFKQSKKPKNG"
     gene            26114..26590
                     /gene="pezA"
                     /locus_tag="AC1PIW_000388"
     CDS             26114..26590
                     /gene="pezA"
                     /locus_tag="AC1PIW_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013851913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PezA"
                     /protein_id="HIP6605352.1"
                     /translation="MIGDNIKSLRRTHDLTQPEFAKMVGISRNSLSRYENGTSTVSTE
                     LIDRICQKFNVSYVDIVGEDKMLTPVEDYHLTLKVEVIKERGAAILSKLYRYQDSQDI
                     VFDDESNPWILMSDDLAELINTKIYLVATFDEIERYNGYLDGIERMLDMVHHRVVA"
     gene            26590..27357
                     /gene="pezT"
                     /locus_tag="AC1PIW_000389"
     CDS             26590..27357
                     /gene="pezT"
                     /locus_tag="AC1PIW_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin PezT"
                     /protein_id="HIP6605353.1"
                     /translation="MRLEEFSEAEFQKALQRTIRALTRGKTIPDQPKAILLGGQSGAG
                     KTTIHRIKQKEFQGNIIIIDGDSYRSQHPNYLALQEKYGKDSVDYTKGFAGKMVEQLV
                     DELSTQGYHLLIEGTLRTTQVPRQTAQLLSSKGYQVSLAVIGTKPELSYLSTLIRYEE
                     LYAIDPTQARATPKEHHDGIVENLVDNLRELESENLFDQIQIYQRDRTCIYDSETAEG
                     SAAEVLQECLFGKWSKVEEEMVKESELLLKELLEKNK"
BASE COUNT         8761 a         5340 c         6418 g         6942 t
ORIGIN      
        1 tagttatgga gaaggaaaaa ctaaaaatac ttgaagaatt aagacgtatt ttaaacaata
       61 aaaatgaagc aattattatc ttgaataatt actttaaagg tggtgttgga aagtccaaat
      121 tatcgactat gattttgatt tatatcaaat agaatctcaa taacggtgag ccacaagcac
      181 aacatggagc aattacccga aatcctaatc aaatcatttc aactggcgat gccatgctgg
      241 gtggctactc aagaatattg gattcaacag gattctttgt ctatgatagc ttcaaaacag
      301 gtgagaccct tagttttaac tatcaaaatc ttcaacatgc acgatttgat ggtaaaaaga
      361 ttagtcgagt gacttacgat attaccaacc ttgtatcacc tgctggaacc aatgccgtga
      421 aattggttgt accaaacgat cccacagagg gctttattgc ctatcgaaat gacggaaacg
      481 gtgactggcg aacagacaag atggagtttc gtgtagttgc taagtatttc ttggaagacg
      541 gttcacaagt taccttctcc aaagaaaagc caggagtctt cacacattct tccctcaatc
      601 ataatgacat tggtctagaa tatgtcaaag atacttctgg gaaatttgtg ccgattaatg
      661 gttctaccgt tcaagtgact aatgaaggtc tagcacgttc tttgggttcc aaccgtgcaa
      721 gtgatttgaa tttacctgag gaatgggata ccacatcaag tcgttatgct tataaaggag
      781 caattgtctc aacagttaca tcaggaaata cctacacagt cacctttggc caaggcgata
      841 tgccacagaa tgttggattg tcttactggt ttgccttaaa taccctacca gttgcacgta
      901 cagtgacacc ttatagtccc aaacctcatg tagcggtgga acttgaaccc gttccagaac
      961 ctattacggt aacaccagat gtctttactc ctaaaacatt tacaccagaa aagcctgtaa
     1021 ccttcacgcc aaagcctttg gatgaagtag tgcagcctaa tctgaccttg actaaggtaa
     1081 ccttacctgt caaacctatt ccaaaagaac ttccaacgcc accacaagta ccaactgtcc
     1141 attatcatgc gtaccgtttg acgacaactc cagagattat gaaagaagtg gtcaatagtg
     1201 accaagctaa tcttcatgag aaaactgtcg caaaagattc aacggtgatt tatcccttaa
     1261 cagttgatgc cttatcgccc aatcgtgccc aaacgactag tctcattttt gaggactact
     1321 tgcctgctgg ttacctcttt gataaggaaa caacacaaaa agagaatgga aactatgtcc
     1381 ttagctttga tgagactaag aattttgtga ccttaaccgc aaaggataac ttgttgcagg
     1441 aggtaaataa agatttaacc aaggtttatc aactgaacgc tccaaaactc tatggttctg
     1501 ttcaaaatga tggggcaacc tattccaata gttacaaact ccttttgaac aagggcacaa
     1561 ccaatgctta cacagtcact tcaaatgttg taacggttcg tacaccaggt gatggggaga
     1621 caaccacact cattacacca gataaaaaca atgaaaatgc ggatggtgtc ctcattaatg
     1681 acatggtcgt agcctttggc acaaccaacc actaccgatt gatttgggat ttggaccagt
     1741 ataagggaga tcgttctgct aaagagacaa ttgcacgagg cttcttcttt gtggacgatt
     1801 acccagagga agtgcttgat atggtggaaa atggcacggg tgttacaacc cttgatggtc
     1861 agaaggtatc aggaataacg gttaaaaact atgcttccct aaatgaagca cctaaagacc
     1921 ttcaagataa attagctcgt gctaagatta caccgacagg tgcctttcaa gtctttttgc
     1981 cggatgacaa ccaagccttt tatgaccagt atgttcaaac tggaatatct ttagctttac
     2041 tgaccaaaat gacagttaaa gatagtctct atggtcagac aaagacctat gcaaacaagg
     2101 cttatcaagt tgatttcggc aatggctatg aaaccaagga agtgaccaac acccttgttt
     2161 ctccagaacc caagaaacaa aacctcaata aagacaaagt agacatcaat gggaagccca
     2221 tgctagtggg aactcaaaat cactatactc tctcatggga tttggaccaa taccgaggga
     2281 ttaaagcaga caactctcag gtttcacaag gtttttactt tgtggatgat tatccagaag
     2341 aagttttatt gccggatgaa gcagctattc agtttgtcac atctgatggc aaaacagttt
     2401 caggaatcac ggtgaagtct tattctcaat tatcagaagc tcctaaatct ttacaaacag
     2461 ccctttccaa acaaaaaatt cagcctaaag gagcttttca agttttcatg cctgaggacc
     2521 cacaagcttt ttttgaatct tatgtgacca agggggagaa tattaccatt gtcactccga
     2581 tgacagtttt ggaaaccatg cttaattcag ggaagtctta tgaaaacgtg gcctatcagg
     2641 tggattttgg ccaagcttat gaaaccaaaa cggtgaccaa ttttgtccct aaagtaactc
     2701 cacataagtc taataccaac caagaaggta tttcaattga tggaaagact gttcttccga
     2761 atacggtcaa ttattacaaa attgtcttgg attacagtca atacaaggac atggtcgtga
     2821 cggatgatgt tcttgccaag ggattttaca tggtagacga ttacccagaa gaagccctta
     2881 ccctaattcc tgatggcatt caagttttgg ataaggatgg caatcgtgta tctggtatct
     2941 ctgtcagcac ctacgctagt ttgtcagaag ctccgaaagt cgtccaagat gccatggcta
     3001 aacgtcagtt tacacctaaa ggagccattc aggtccttag tagcgatgat cccaaaacct
     3061 tttatgatac ctatgtgaag actggtcaaa ccttagttgt cacgcttccg atgacggtaa
     3121 aaaatgagtt gaccaaaaca ggtggtcagt atgaaaatac agcctatcag attgattttg
     3181 gcttggccta tgtcacggaa gcagtagtta ataatgttcc aaaattagac ccacaaaaag
     3241 atgtggtaat cgacttgtct cataaagatg ccagccttga cgggaaagaa gtggccttgc
     3301 atcaaacctt taactatcgc ttggttggag cattgattcc aagcaatcgt gcgactgatt
     3361 tatttgaata tggttttgaa gataactatg atgaaaagca tgatgagtat aatggtgttt
     3421 atcgcagcta tctgatgaag gatgtcatcc tcaaagacgg ttctgtatta aaagagggga
     3481 cagaagtcac gaaatatacc ttgcaacagg tggatacaga aaatggccta gtgtcaattt
     3541 catttgataa atccttctta gagactatct ctgatgattc agcttttcag gcagatgttt
     3601 acctgcagat gaaacggatt gcgactggtc aggtggagaa tacctacctt catacagtga
     3661 atggctatgt catcagctca aatacagttg taacatatac acctcaacct gaacaaccaa
     3721 gtccaaatca acccacacca cctcaaccac caattgagac tattgaagcg cctgtttcag
     3781 caagcatttt gccaaataca ggggaacagg aatccctttt gggcttgatt ggagctggta
     3841 ttctacttgg tacggcttat ggactgaaga aaaaggagga gaagtagatg aatccaaaaa
     3901 gcatttatga aaaggattca gaccaggatg gtctgacaga tgctcaggaa ctggctttcg
     3961 gaaccaatcc gcagtctgtt gacacagatg gtgatggtca agctgattta gaagagctac
     4021 aatctggaca ttcaccatta gtcccacaaa aggagttgtg tgatggcttg gaactttgac
     4081 accatgaaag aggctttgtc tgaaatggag aaggtcgatt accaagagtt tatcaaagcc
     4141 tttctctctt tggaattaag tatttctgat agaacaatcc tcaatcaggt ttatcaagat
     4201 tacatggatg aggatgacct atccttgatt agtgatgagc tacgagttaa agttgatagt
     4261 tatcaggatg aagtacaggc agatatgact gacattctag aaaagttgta ccgaacaggt
     4321 gaaggctcta gttttattat ggatttaatg tcctcaaata gtctctcaga caccttggaa
     4381 cagtatgagg ttttggatag tgacgattat tcaccactta gccttgaaac cttacaggcc
     4441 atgatccaac aggacttagc tatttccagt caggattatt ttggagatct ggttcacctt
     4501 gccttgcaaa aagatttact agaccagaaa agtcatttct tacaacacta tgtggcaact
     4561 gtaatggaag gtattccaca agaaagagac caacgagcct tggtcctaga ttaacgcaaa
     4621 gggctgagaa aaactctcag cccttttaaa atgggaggga aaatgaatca agaagtctta
     4681 ctacaaatga tgagagccac cattcctcgt gatagagccc tgcttgaggc atttttatat
     4741 taccaagcag agcattttga tgaggagtgg gatagtctta ttcgtcagtt tttatccaat
     4801 aggcaagcaa acatcagccc tgttcaggta cttcactttg agacagatgt ttcagctttt
     4861 gtccaggcta gtccttatga taatgctcat gatctattga cctatacaca agtattcggc
     4921 caaactggtc tccaaaaact cgacaaacta tcgccgtctg aaaaagactt ggtgattgaa
     4981 gtggccttgt tcaatctggc cactcgtttt caactattag actcaaatgg acactaccaa
     5041 actatttcac cagattctct cttacaaaag agtaggggag ctaatttggt caatgtttat
     5101 cgtgtggcta ataatttagc ggatcgaatc agccgagata ttgaacagtt tctcttgact
     5161 tacgagcctg agcttgaaac aagagctgat aaaactgttc tagaaaatga agaaactggt
     5221 gatgagcaca aagtgagtag taatcaagca ataccattta gagaagatgg ctctctcatc
     5281 attgctagtt tggatgtaga tttgtctcaa ctgaatgttc aaacaggaaa aaccagtcat
     5341 ctgccagctt atgaagagtt gttattacaa cgtaaatttg agattctaac atattttgac
     5401 catattcgaa atgaacgttc caaagtccca agttttaaac gaggtgattt tgatactgag
     5461 atggaaatga caccaatctt tgagggcaag gagttactta cctatctaga agctgatggc
     5521 agtccctatg agctgaaacg aacgctgact acagtcgaag aaaaggaatt agaaaaaatt
     5581 ggacaagcca ttaggataga aaatcaagaa aaattgactc agctagggat tgagctatct
     5641 cagtttgacc cagaccgagt cggtatttta ttggatgcgg caggtcgtta tcgtttaaaa
     5701 aatgcggacc ttgctttact aggtggttat cccaaagcct cggtaactca actagccctt
     5761 gcgacagaac tactccaaat gggactaagt catgaaaagg ttgaattttt ctttggtagc
     5821 cagctttccc ttgaagagtt gcgacaagtt gcttacgcct ttttacacga agaactcagc
     5881 agagaagatg cggagcaatt tgaaaaagat aaaggtaatc agccagattt aaccctcaga
     5941 gattggaaga gcaagctaga gaaagctgag agaaaagaag tagttgatga agaatttgtg
     6001 gaaaatccac tagtccagag agtattggac acttatcctc tggggtcatt ggtttcctat
     6061 aagggacagg actttgaggt cgtgtcggtc agtgatgctc gattgaacgg tttgattcgg
     6121 attgagttag tcaatgactt ttcggatatc attgaacaaa atccagttct ttatgtgagg
     6181 acctgggaag aagtcagtca ggcacttcat cagccaaagg cagaaccaca aacagagtta
     6241 gaagaagcgg accaagaact aaatcttttt tcatttctgg aagaggagcc agttcagagt
     6301 attggactat tggaaccaga tggttcagaa aaaggtcata acgatactga tcttgaagaa
     6361 acagataatc aaattcctga agaggaagtc gtcgaaacaa ttccagagat tccagtaacg
     6421 gacttttatt ttccagaaga tatgacggac ttttatccta agactgctag agataaggtt
     6481 gagacaaaca ttgcggccat tcgtttggtt aaaacacttg aaacgggacg ccgtcaggca
     6541 agtacaagtg aacaagaact ccttgccaag tatgtgggct ggggtggact agccaatgat
     6601 ttctttgatg actataatcc aaaattttct aaggaacgag aagaactgaa aagcatagtc
     6661 acagataaag agtattcgga tatgaaacag tcctctctga cagcctatta cacagaccca
     6721 gccctgatcc gtcagatgtg ggataagttg gaaagagatg gctttacagg tggcaaaatc
     6781 ctagatcctt ccatgggaac agggaatttt tttgcagcaa tgcccaaaca cttaagagaa
     6841 aagagtgagt tatatggtgt agagttagac accatcacag gagctatagc caaacacctt
     6901 catcccaata gtcacattga aattaagggc tttgagacgg tagcttttaa cgacaatagt
     6961 tttgatttgg tgatttcaaa tgtgccgttt gccaatatac gaattgcgga taacaggtac
     7021 gataagcctt acatgattca tgactacttt gtcaaaaagt cacttgattt ggtccatgat
     7081 ggtggacaag tagcgattat ctcttccaca ggaactatgg ataagcgaac agaaaatatt
     7141 ttacaagata ttcgtgagac aactgaattt cttggtgggg ttcgactgcc tgattctgcc
     7201 tttaaggcca ttgcgggaac gagtgtcaca acggatatgt tattcttcca gaaacacctg
     7261 aacaaggaat atgtggcaga cgactcagcc ttttcaggtt ccattcgcta tgacaaggat
     7321 agtcgcattt ggctcaatct ttactttgat ggagaataca atagccaagt gctaggaacc
     7381 tacgaggtca ggaattttaa cggaggaaca ctttctgtta aggggactag tgatgacttg
     7441 attgcaagtg ttgaagcagc tctacatcaa gtaaaagccc caagagagat tgatagaaat
     7501 gaggtcatca ttaatccaga tgtgttgatc aaacaagtca ttgatacctc cattccagct
     7561 gaaatgaggg agaatctggg tcagtacagt tttggttacc agggttctac agtttactat
     7621 cgagataata aaggcattcg agtcggaacc aagacggaag aaatcagtta ctatgtcgat
     7681 gaagagggca acttcaaagc atgggacacc aaacattctc aaaagcagat tgatcgcttt
     7741 aatgccttag aagtgactga taacactgct ctggatgtct atgtgacaga tgatgcagcc
     7801 aaacgtggtc agtttaaggg atattataaa aagacagttt tctatgaagc tcctttgtct
     7861 gataaagaag tggcacgaat caaaggaatg gtcgatattc gcaatgctta ccaagaagtc
     7921 attgccattc aacgctatta tgactatgat aagaagacct ttaaccactt gttaggtaaa
     7981 ctcaatcgta gctatgatag ctttgtcaaa cgctatgggt atttgaatag tgctgtgaac
     8041 cgcaatcttt ttgatagtga tgacaagtat tcgcttcttg ctagtttgga agatgaaagt
     8101 ccggttccaa gtggaaactc cgttatctac accaagtccc ttgcttttga aaaggctctg
     8161 gtgcgtccta aaaaagaggt taaaaaggta catactgccc ttgatgcctt aaattcgagc
     8221 ttggctgatg gtcgaggtgt tgatttcgat tatatgatgt ctatctatca ggttgaatcg
     8281 aagatgaact tgattgagga gttaggtgac ctcattatgc ctgatcctga gaagtatttg
     8341 aatggggagc tgacctatgt ttctcgccta gactttctgt caggtgatgt cgttaccaag
     8401 ttagaagtgg tggacctatt tgtcaaacaa gacaatcagg actttaactg gtcacattat
     8461 gcgggacttc tagaagctat caaaccagcc cgtattactt tggcagacat tgattatcga
     8521 atcggttcac gctggattcc tttgtctgtt tatggaaaat ttgcccaaga aacctttatg
     8581 gggaatgcct atgaattatc ggaccaagaa gtagcgacag tcctagaagt cagtcccatt
     8641 gacggggtta tcacttacca atctaagttt gcctacacct attccaacgc aatggatagg
     8701 agtttaggtg tccctgcttc acgctatgat agtggtcgaa aaatcttcga aaatctcctg
     8761 aattccaatc aaccaacgat cacaaaacaa attgttgaag gggataagaa aaagaatgtg
     8821 acggatgtag agaaaacaac ggtcctgcgt gccaaggaaa cacacctaca ggaattcttt
     8881 caagattttg tagcaaggta tccagaagtc caacagatga ttgaagacac ctataacagt
     8941 ctctataatc gtacagtatc aaagacctat gatggtagtc atttaaccat tgatggactt
     9001 gcccagaata tctccttacg tcctcaccaa aagaatgcca ttcaacgaat tgtggaggaa
     9061 aaacgtgccc tactagccca cgaggtaggg tcaggaaaaa ccttgaccat gcttggagct
     9121 ggtttcaagt tgaaagaatt agggatggtg cataaacctc tctatgtggt tccttctagt
     9181 ttgactgccc agtttggtca agaaatcatg aaatttttcc ctaccaagaa agtctatgtg
     9241 actactaaga aagactttgc caaagccaaa cgtaagcagt ttgtgtctcg gattattaca
     9301 ggggactatg atgccattgt cattggggat tcccagtttg agaaaatacc gatgagtcgt
     9361 gaaaaacagg tcacctatat caatgacaaa cttgagcaac tcagagaaat taaactagga
     9421 agtgactctg attacacggt gaaagaagcg gaacgttcga ttaagggatt ggaacaccag
     9481 ttggaagaac tccaaaaact agagcgagat acctttattg aatttgaaaa tcttggaatt
     9541 gattttcttt ttgtggatga ggctcatcac ttcaagaata tccgtccaat cactggactt
     9601 gggaatgtcg cgggaatcac caatactact tcaaaaaaga acgtagacat ggagatgaag
     9661 gtgagacagg ttcaggcaga acatggagat agaaatgtcg tttttgcgac aggaacgcca
     9721 gtttctaact ctattagtga acttttcacc atgatgaatt acattcaacc tgatgtttta
     9781 gagcgatacc aggtatccaa ttttgattca tgggttgggg cttttgggaa tattgaaaac
     9841 tccatggaac tagccccgac aggtgataaa taccaaccta agaaacggtt taagaaattt
     9901 gtcaacttgc ctgaactcat gcgaatctat aaggaaactg ccgatattca gacctcagat
     9961 atgctcgact tacctgtacc tgaagctaag attattgcgg tggaaagcga gttaacgcaa
    10021 gctcagaaat actatttaga agagctggta gagcgttcag acgctatcaa gtcaggtagt
    10081 gttgctccaa gtagagataa catgcttaag ataactggag aagccagaaa actagccatt
    10141 gacatgcgtc tcattgatcc tgcttacacc ttgtctgaca atcagaagat tcttcaagta
    10201 gtggacaatg tggaacgcat ttaccgtgag ggagaaaatg agaaagctac ccagatgatt
    10261 ttctcagata ttggaacgcc taagagtaag gaagaaggct ttgatgttta caatgaattg
    10321 aaagaccttt tagtggatag agggattccc aaagaagcaa tagcctttgt ccatgacgct
    10381 aatacggatg acaagaaaaa ctctctgtca cgcaaggtca atagtggaga agtacggatt
    10441 ctcatggctt cgacagaaaa agggggaaca ggattaaacg tccagtctcg catgaaagct
    10501 gtccaccatt tagacgttcc ctggaggccc tcagatgtag gacggatttt gcggacattc
    10561 aaaataaaaa agaatgtgga ggtaacagac aatggcaaag acaatttttg aggaaatggg
    10621 cggcaaatac gaaaggcaag gcgattattt aataccgtgc ttaactgtac ccgccgaaga
    10681 agaacagccg ataggcatct ggggacagcg gcatttggat tatctgaagc atcactgcaa
    10741 agttacatac accaatcttc ttacaagcgg cagacttaac gcttaccttg ccgacattga
    10801 cagacaggca caggaacgct ttgaaaggct catagagggt atgaaacagg cacagggcat
    10861 aacggaacag ctaaaggcag aaaacgcctt agaatggaca ggatgcctca ataacataag
    10921 ggcttgtgcg agggagattg tggaaaagga aattattttt gcataaacag atgattagtg
    10981 gcagggggaa atcctgccgc tttttctgct ttagtttgtc agcttgacaa ataaagggtt
    11041 aaggaatata attagattca gtattataca aggagttaat aaatatgcgg caaggtattc
    11101 ttaaataaac tgtcaatttg atagtgggaa caaaaagtag cagtcccgtt tcacttttaa
    11161 tatggggctt agttttttgt acccagttta agaatacttt tatcatgtaa ttttatatgc
    11221 ccgaaaacat ataagtgttt tggggctatt ggagttattt acccagtgat aggagtattt
    11281 atcactgggt atttttatgc ccttttttgg gtgttgatag gaggaaaatc acatgaaaat
    11341 aattaactta ggcattctgg ctcacgttga cgcaggaaag acaacattaa cggagagttt
    11401 attgtatacc agtggtgcaa ttgcagaacc agggagcgta gataaaggca caacaaggac
    11461 agatacaatg aatttggagc gtcaaagggg aatcactatc cagacagcag tgacatcttt
    11521 tcagtgggag gatgtaaaag tcaacattat agatacgcca ggccatatgg attttttggc
    11581 ggaagtatac cgttctttat ccgtattaga cggagcagta ttattagttt ctgcaaagga
    11641 tggcatacag gcacagaccc gtatactgtt tcatgcacta cagacaatga agattccgac
    11701 aatttttttc atcaataaaa ttgaccaaga ggggattgat ttgccaatgg tatatcaaga
    11761 aatgaaagca aagctttctt cggaaattat agtgaagcaa aaggttgggc agcatcccca
    11821 tataaatgta acggacaatg acgatatgga acagtgggat gcggtaatta tgggaaacga
    11881 tgaactatta gagaaatata tgtcagggaa accgtttaaa atgtcagaac tggaacagga
    11941 agaaaacagg agattccaaa acggaacgtt atttcccgtt tatcacggaa gtgctaaaaa
    12001 caatctgggg attcggcagc ttatagaagt gattgccagt aagttttatt catcaacgcc
    12061 tgaaggtcaa tctgaactat gcgggcaggt ttttaagatt gaatattcag agaaaaggcg
    12121 gcgttttgtt tatgtgcgta tatatagcgg aacattgcat ttgagggatg ttattaaaat
    12181 atctgaaaaa gagaaaataa aaatcacaga gatgtgtgtt ccgacaaacg gtgaattata
    12241 ttcatccgat acagcctgct ctggtgatat tgtaatttta ccaaatgatg ttttgcagct
    12301 aaacagtatt ttggggaacg aaatgctgtt gccgcagaga aaatttattg aaaatcctct
    12361 ccctatgctc caaacaacga ttgcagtaaa gaaatctgaa cagcgggaaa tattgcttgg
    12421 ggcacttaca gaaatttcag atggcgaccc tcttttaaaa tattatgtgg atactacaac
    12481 gcatgagatt atactttctt ttttggggaa tgtgcagatg gaagtcattt gtgccatcct
    12541 tgaggaaaaa taccatgtgg aggcagaaat aaaagagcct actgttatat atatggaaag
    12601 accgcttaga aaagcagaat ataccatcca catagaagtc ccgccaaatc ctttctgggc
    12661 ttctgtcggg ttgtccatag agccgctccc tattggaagc ggagtgcagt atgaaagcag
    12721 agtttcactt ggatatttaa accaatcgtt ccaaaatgcg gttatggagg gggttcttta
    12781 tggctgcgag caggggctgt atggatggaa agtgacagac tgtaaaatct gttttgaata
    12841 tggattgtat tatagtcctg taagtacccc cgcagacttt cggctgcttt cccctatcgt
    12901 attggagcag gctttaaaaa aagcagggac agaactatta gagccatatc tccactttga
    12961 aatttatgca ccgcaggaat atctctcacg ggcgtatcat gatgccccaa ggtattgtgc
    13021 agatattgta agtactcagg taaagaatga cgaggtcatt ctgaaaggag aaatccctgc
    13081 cagatgtatt caagaataca ggaacgattt aacttatttc acaaatgggc agggagtctg
    13141 cttgacagag ttaaaaggat accagccagc tattggtaaa tttatttgcc aaccccgccg
    13201 cccgaatagc cgtatagata aggttcggca tatgttccac aagttagctt aacagcttgc
    13261 aaaagtcata taaaatgaga tttgaaagga ttagagacta attatgatga aatgcgaatg
    13321 gatattgtgt cctgtttgtg ggagcaaaac ccataataaa attaggaagg acactgtttt
    13381 ggagaattat cccctttatt gtccaaaatg cagacaagaa agcttgatta aagttgacaa
    13441 cttgaagata actgtcatca aagagccaga cgcttaagac gcatagccga tgaaattgtg
    13501 gaacaattca cgaatcatcg gctctttttg tttcgtattt gaaagaacaa actcaccaaa
    13561 taaaaaaaac gatattcggg tgggttattt tgttataccc taaattaccc tctgaatttg
    13621 tttttaaatt tggagggatt tttttatgtt cttttttcgg gcggttattc atctgctcat
    13681 acgaagtaaa atttatcaat acatagcata tcagagaatg gcaggaaacc agttaaaaaa
    13741 atttctgcca gtgcagcggt acttctcacc ttgaaagtgg aagtacaatc tccatacaac
    13801 agaatttgta tttcccataa ccgtgaaggt cacagagcct ttgcggtttt tcttttgtcg
    13861 ttttttgttg gaaagtgccg cagggctgtt tctggtgtgc gttgccgctc cccgcctctc
    13921 catctggatt tttacatttc aaaaattcag attggaggta ttagcggtga aatacgcacc
    13981 aagaaaagta tatatcaaag aaagcggcgg ctatgtggaa ctgtcctata cggatttctg
    14041 ccgccgcagg caagccgaca agggatatat ggacaagctg tttatccccg tccaaggttg
    14101 tctgcttgaa gtcgtaaggg agcaatatgc ggacttctac cgtgacaggg agcggtggcg
    14161 ttacctgcaa aaattagatg cgaggaacag cctgctatct ctggacggat ttacggacag
    14221 cgaggggaat cctctggact ttatcgctga tgaagcggcg gacattgcgg aaaccgttgt
    14281 caatgctgtt atggtggaca ggctgaaagc cgccctgcct ttgttgtcgg atagtgagca
    14341 ggagttgata caggcaatct tttttgacgg actttccgag cgtgaagtcg gggcgaggtt
    14401 aggcataacc cagagcgttg tgaacaaacg caaagccaga atcctaagaa aactaagaaa
    14461 gataatagaa aattaaaatt taaggcgttc agcccccttg ttttttcctt tgggaaaatg
    14521 agggggcttt tctctgccct ctcaatcaat tctgattgga ggaagaaaga atggcataca
    14581 accacggacg ggaggacagg aaatggcgta tctggaaaga agcggaggaa aagctgctgc
    14641 gtgagtgcgg cgttgatgaa gtgaccattg agcagatacg catagcggac agggcagact
    14701 tcaattccaa caggcggttt taccgatgga cgaatgacgt tgcggaatac cttgaggaca
    14761 tggcagacag ggagcggcaa gcggaggtga atacagttgc ggagttactg gacgagattg
    14821 agagcgaaaa tctctatcaa gtattagtca cggtggacgg gcgtaccttg aaaatcgtcc
    14881 tgctgaaaat gcaggggtat tccacaaagg agattgcccc acttgtgcat ttgacgactg
    14941 gtgccatcta tgcgaggtta gaccatctgc ggaagaagct tcggaaaatt ttgtaagcgt
    15001 ctaatacatc ccattattct gcgggctact gggtggggag tggaatctcc ccgcttattt
    15061 ttggcaggag gaaaacggga tggcatataa agctaaggcg tacacgcttc gggaggaatc
    15121 cacggaaagc ggtataaggt attttatcag ttttaaggac gggcaggggg aacaccacga
    15181 actggaagta tccgagcagt tattctttga atttcggcag atggaacgca ggaacaggaa
    15241 tcttctccaa tgggacgagc ggcacaggga gttttcggaa gtatgggacg aaacgctgaa
    15301 caggcgggcg ttaaagctgc ctaagagcat tgaggaacaa atgattgaag cagaacgggc
    15361 ggaactgctc tgtaaggcgg ttgacagact gccagagata caaaggcgac gtttcctgct
    15421 ctactacgag tatgagttca atttttacca aatcgccgct atggagcatt gcaccgcttc
    15481 tgcaatacag aaatccgttg ctgttgcaaa gaagaaagta aaagcggaaa tgaggaaata
    15541 tctccaacca tgaccgacac cgcccgaaaa agaaatctgt tttttatttg tgtgggattg
    15601 gcggtattac atactctcac ttttttcgtg ggagtaaatc tatttttaag gaggtcaacg
    15661 cctatgtgca acaaaaacaa gataactgcc cgaaaggagg aaacccatgc agaagttaca
    15721 gacagtcaac gccgaaacgc tcctttatga gccgcttgag aagccgtcct ttgtggtgga
    15781 cagccttatc cccacaggct tgtcgctgtt ctgcggctca cagaagatag gcaagagctg
    15841 gctcatgctg aagctgtgct tatgcgtgtc gcagggaatc cccttatggg acatgacgac
    15901 aatggagggc gatgtgcttt acctctgcct tgaggacacg ttctgccgca tacaggacag
    15961 gctgttccgt ttgacggacg aagcgagcgg gcggcttcat tttgccgtgg caagctgcaa
    16021 gctgtcagac ggtcttatcg tgcagcttga ggattatctg aaagactatc cagacagcag
    16081 gctcattgtc attgatacct tgcagaaagt ccgtacagct tcaaaagaca acgcctatgc
    16141 aagcgactat ggggacatct ccctaatcaa agattttgcc gacaggcact ctctggcggt
    16201 cattgtcgta caccacatcc gaaagcagaa tgacagcgat gtgttcaaca aggtgtctgg
    16261 gacgacaggc ttaacgggga gtgcggacgc tacctttgtt ctggaaaagg agaaacgggc
    16321 atctgacacc gccaagctgt atgtaacggg cagggacacg ccctatcagg aatacacgct
    16381 ccgtttccgt gattgcagtt gggaacttgt ggagaggaaa acgcaggagc agcttgcgaa
    16441 agaaacgata ccagatgtcc tttttcggtt ggtggatttt atgagggata aggaagaatg
    16501 ggcaggcacg gcaacggagc tgttagccgc tatgggggaa acggaaacca tacccacggt
    16561 gattacgaaa tggctgaatg aatatcacac cacattttta agcgagaacc gtatctgcta
    16621 ccagtacagc cgcaggaaag acggcaggca gattgccctt gcaaggaggg cgggtgacag
    16681 cggtgacggc ggtgacagcg atattgggat accccctgtt actgtcattg acgcttaaag
    16741 ccatgtaaag ttggcggctc tgccctgcgg gtgacagcgg tgacggcagt gacagtgatt
    16801 ttaggatacc ctgccgctgt catccctacg ggagaacacc ccgcaaacgc aaaatgcagg
    16861 cgtgggattc acccgccctt ttcgggcgtg taaaatcacc cctgcggtca gaaaaatctc
    16921 tccgattttt ccgactgcgt ttacagggtg taacacacta cactttgccc tgcaaagagc
    16981 cgtgtgccag acgttccctc tggactccct tatggcaggt cttacgccct gctatcctac
    17041 gccttgcggc ttcggataaa agaacggcgg catgacggtt acagctcttg cgagggagta
    17101 tcccaaaaac accgtcacca tcgtcatgac cgtcatgcag gggataaggg tgacagttac
    17161 ggcaatcggc agggggcatc ccaaaactgc cgtcaccacc gtcaccgctg tcacccaaag
    17221 ggcagttacc cgccgaagaa aggaagatga tggattatgc cctatgcaat cctgcgtttc
    17281 cagaaacgca aagcgggcgg cgttgcggct tgcgaacgcc acaacgagcg gaagaaagaa
    17341 gcctacaaaa gcaacccaga tatcgacatg gaacgctcta aaaataacta tcatcttgtc
    17401 gcaccgccaa agtacaccta taagaaagaa attaaccgaa aggtagccga agcagggtgc
    17461 aggacaagga aagacagcgt gatgatggtg gaaacgctca tcacggcttc accagaattt
    17521 atgaaccagt taccgcccga agaacagaaa gcgtatttta cgatggctct ggatttcatt
    17581 tcggagcgtg tcggggagca gaatatcctc tccgcagtcg tccacatgga cgagagaacg
    17641 ccccatatgc acctctgctt tgtgccgatt acaccagaca ataagctgtc cgccaaaaca
    17701 attttaggca atcaaaagag cctgtcggag tggcagaccg cctaccatga gcggatgtcc
    17761 tcacgttgga atcagcttga gagaggtcaa tcttcgatgg aaaccaagcg gaaacacgtc
    17821 cccacatggc tctataagtt gggcggcagg cttgataagc agtatgggga aatcgtgtct
    17881 gccctgtccg acatcaacgc ctttaacgca gggaaaaagc gtgacaaggc tctggaactg
    17941 gttgccgcat ggctgcccga agtggagaaa ttctctaagg aaatcggcag acagcaggcg
    18001 tatatcgaca gcttaaagga gcaaatcggg caggaagccg actatgcagg gcgtatgcgt
    18061 gatgaaaagt acgagcagga attaaaggtg cagaaagcca accagaggat atttgaattg
    18121 cagagaacca acgagcagat ggggcggctg ctctcaaaga taccgccaga agtgttggaa
    18181 gaattacaga gaacaggcag aaacaaatca agagaaaggt aaaaagtgaa tgaagaaaca
    18241 ggattttaag gtgttaaaga ccaaagattt atacccgttt cccgacaatc cgtttcatgt
    18301 tgtggaggat gaaacgctgt cagagttagc ggaaagcatc aaggaatttg gcattgtcac
    18361 gccgataatc acacgcccga aagaggacgg gaacggttat gaagtgattg cggggcagcg
    18421 gcgtgtccgt gcgtccgaac ttgcaggtat aaataccgtg cctgcatttg tcctgccctt
    18481 agaccgtgac cgagccatca tcacccttgt agacagtaat ttacagcgtg aaaatatcct
    18541 gccatcggag cgggcgtttg cctacaggat gaaatccgaa gccatgaagc ggcagggttt
    18601 ccgcacagac ttaacctcgt cacaagttgt gacgaagttg cggacggacg acaaagtggc
    18661 acagggcttc ggcgtgggca ggatgaccgt ccagcgtttt atccgcctga cggaactgat
    18721 accgccgatt ttgcagatgg tggacgaggg gaaaatcgcc ctcacacctg cggtggaact
    18781 gtcctttttg aagaaagacg agcaggaaaa tctctttgcc acgatggaga gcgaagaagc
    18841 aacgccctca ctctcacagg cacagcggat gaaaagcatg agccagagcg ggcagcttga
    18901 catggatatg atattttcca ttatgacgga ggaaaaggga aaccagaaag aaaccttgaa
    18961 aatcaacaca agcaaactga aaaaatactt tccgaaggac acaacgccga agcagatgga
    19021 ggaaaccatc atccgacttc tggaacgtga gttgcagaga aaacggagca gggacagccg
    19081 ctaaccttct tttttcggga agtaaatgca ggaatttgga acagaagaaa gacagaattt
    19141 taaggagaat gaggtaagga atgaaagaaa tccagtatga gattgtaaag gaaatcgccg
    19201 tattgtctgc gagtgacagc ggctacacaa aggaaatcaa tcttatttca tggaacggga
    19261 gagagccgaa atatgacatc cgcagctttt ccccgaaccg tgagaagtgc ggaaagggta
    19321 tcacgttgaa cgctgatgaa gcagcggcac tccttgaagc attacagaaa gaagtaaaca
    19381 gcggggattg atggtatctg attgacaggg cggggcgttt ccagacgttc tcctgccctg
    19441 tctggaaagg aagatttaag tatgggcgag gataggaaaa caactggaag aagaaagcgt
    19501 attgtgccaa aagggcaagt acaaatgatt atcagtcgag aatatatcgg cacacagacc
    19561 gttgcagaag cgtttgtccc gattatctcc gaggatattc ggaagaagat tgccgagggc
    19621 gacaccttcg acaatgaggg gctgtccgct tagaatgtac gcaatggaac atgaaaacgt
    19681 taggacagat atggaggttt tacagtatgg caggaataag aacggagaac aagatttatg
    19741 aagtcggcat ttactgccgc ttatcaaagg acgatggcac ggataacgag agtgcgagca
    19801 ttgcgacaca aaaatccatc ctcacggatt atgtgaaaag gcagggatgg catttagcaa
    19861 aaacgtatgt tgacgatggc tactctggaa caaatttcca aagaccaagt ttccagaata
    19921 tgattaagga cattgaaaac gggctgataa actgcgtgat tacaaaagat ttatcccgtc
    19981 tggggaggaa ctatcttgat tgtgggttat atctggaagt ctttttccca gagcataacg
    20041 tgaggtatat agcggtcaat gacggcgtgg acaccttaaa caaatccgct atggacatca
    20101 cgcctttccg caacatccta aacgaaatgt attctgccga tgtgtcagtc aagataaaat
    20161 cggcgtaccg tgcgaggttt cagcagggga aattcatggg aacaacagcc ccttatggct
    20221 atgtcaaaga ccctgccgac cacaaccatc tgctgataga tgacaaagtt gcccatgtgg
    20281 taagggagat attcgacctt gcgttagcgg gcaacggcat cgccaaaatc cgcaagcaca
    20341 tcaacaaaca gcatatccta cgccctgccg cttatgcggt ggagcaaggg gcaacaggct
    20401 atgagcggta ctttgaggat aacgaggaaa accgttatat ttggagcgag aacagcgtga
    20461 ggggcatttt aagaagcccg atatatgcgg gcaaccttgc aggctacaag cggattgcag
    20521 ccaacatgaa aagcaagaaa cgcccctcta agctgcccga agaatgggaa gtgataccag
    20581 acacccatga ggggatagtc acgcaggagg aatttgacac cgtacagcag cttatgacga
    20641 gccgcaggcg ggagcagaac gcagggggat ttgagaatat cttttcgggc gttatcaagt
    20701 gtgcggactg cggctatgcg atgcgggcgg cgagtgccaa caggaggaaa cgccccgaca
    20761 tcatcgactg cgtacaatac acctgcaata attatggcag atatggcaat gttatgtgta
    20821 ccgcacacag cattgaagcg agggacttat tcaatgccgt ccttgccgac atcaaccgct
    20881 ttgcggatat ggcggtcaat gatgaaaagg cggtcagagc catagaaagg cggctcacgg
    20941 aaacagacca gagcagggcg aaatcattag agaaagaaaa gaagaagctg aacaaacgcc
    21001 ttgcggagct tgacaggctg ttttcctctc tctatgagga caaggtaatg gagcgtatca
    21061 cggagcggaa ttttgagatg atgtcgggga aataccagaa agagcagctt gaaattgaag
    21121 cacggctgaa agaggtaacg gaaacgctca atgacagcta tgagaaatcg cagggcgtga
    21181 gggactttct atccctaatc cgcaactatc aggggctgaa agagttggac gcaaccatca
    21241 taaacgcact catagacaag atacttgttt cggaacgtga gaaacttgcg gacgggacag
    21301 taaggcagga aatcaagatt tactataaat tcatcggctt tgtcggtgaa ttacatatca
    21361 cacccacaaa gcggtggacg gcgttaaagc ctaaaaattg tacggtgtgc ggtgttgaat
    21421 acgtccccag ctctggaata tcaaagtatt gtcccgcttg tgctaagagg atacagaggg
    21481 agaaatcaaa cgagagcaaa cgcaggagta gggagaggaa ccgaagggca tgtattgaac
    21541 tgtccgcaaa aaatgaccga ctgacattgt ccagcgaaat ggacgactaa ttcgacaagg
    21601 aaacatgcac caggaggtag atatttatca ctatattact aaagggagct tcgacaatta
    21661 cctatggcag acacaggaga ataagctaaa gtatatcacc cagataatga cctcaaaaga
    21721 tcctgtgaga tcagctgaag acattgatga acaaaccatg actgcctcag actttaaggc
    21781 cttggcaact gggaaccctt atctcaaact caaaatggag ttggaaaatg aactgacagt
    21841 tttagagaat caaaaacgag cctttaatcg ctccaaagac gagtatcgcc ataccatttc
    21901 ctatagcgag aagcacctcc ctatcatgga aaaacggttg agtcaatatg ataaagatat
    21961 tgcccaatct ttggcaacaa agtcgcaaga ttttgtcatg cgatttgaca atcaagcaat
    22021 ggataatcgt gctgaagctg gggactatct gcgaaaactc attacctata accgctcaga
    22081 gaccaaggaa gttaaaacac ttgccaactt tagaggattt gatttgaaaa tgacaacacg
    22141 agctcctagt gagcccatgc cagagactgt ctctttaatg attgtaggag ataaccagta
    22201 tactgtcgcc cttgatttga aatcagacgt gggaaccatt caacggatta gcaatgccat
    22261 tgaccatatt atagatgacc aagaaaagac gcacgagctg gtaaaggatt taaaagataa
    22321 gctacgagta gccaaagtag aagttgaaaa agcctttcca aaggaagagg actatcagct
    22381 tgtaaaggct aagtatgatg ttttagctcc cttggttgaa aaagaagcag agattgagga
    22441 gatagatgca gctttggcca agtttagtga agatacaaca ccccaaaaga agcaacaaat
    22501 agcacttgag atataagaaa aaaggctgac aaataatttt gaacgcggta aaatgaagtt
    22561 aagaaatcac ataaggagaa tagatcatgg gccaaacagt agaagaaatg cgttatcaac
    22621 tagaagaatg gttgtcacaa ggctttacga gttcagaaga tagggcaaac taccaaactt
    22681 taaaggaaca gtatgaagat gagacctttg attacagttt ttcaaggcgt gaaattactg
    22741 gacagctgga aatcatcatc acaagtcgtg agaatgattt tccaagctta gataaggtga
    22801 cgaaggcaga ataccttgat ttggttgccc aacttgatga tttagacaag agacaggctg
    22861 actattatcg caagcaatta gcctagaaag aggtgtaaga tgttggagca aattctacaa
    22921 agccttttga ttatcgcagc aataggactg atgttgtttg tcctttatcg gattgtgaaa
    22981 gtttcaggtg ctttatttct tatcggactc atcagtggat tagtctttat tgaaatatat
    23041 ggaatttacc tattcttcac agaaagacac ctatattcag aagatttagc agctaatggc
    23101 atatggagtt tcactggatt ttatattgca ttgaatttat ttcttgctat tagcttaagt
    23161 atgaggtggt ggagaaatag aatcgtttaa catggggatt acctactggt agtccttttt
    23221 cttgtgataa aatctccaag ctaatcttga tatgatgaag ctatcactaa tagaggagta
    23281 agccatgtta aataaagtga aagctcgatt tctgattgga ttaggaggtt taatcgcagt
    23341 cagttttatg gtcatgattg gctacacgat tggctcacaa accatttcaa agcaaacaga
    23401 gaaccaaata cgagcagaag ccaataaact tgtgtcaaag aaaaagcagg aggaaagagc
    23461 gacagtccta tcagatgagc ttgtcaatga atttctcact caatatttca ccaaggtcca
    23521 gctgggtgaa aataacgctc gtatcaaacc ctacatgacg gactcggcat tttcagaaga
    23581 ggaagcaaat cagaataaag cgatcaatca agtttataaa gactatatgc ttgactaccg
    23641 atttgaatca gccagtatct atgtcaatac agaaagtaat gttgcacttg cggaagttac
    23701 ctatcaagtg acctatgtat ctgatttgag tgagcaacaa cagcgaacct ctcaaacaga
    23761 aaccaagacg gttatgttat cctactccaa agtttccgac aagctcctgg ttaatcagtt
    23821 gaccatttgg aatggaaaac tagaggacat gaaagaagcc acagatggtg caaattccag
    23881 cataccaacg atccaaggaa ctacgacaag cgagaacaac tagcaggaga cagtcttctg
    23941 ctttttttga tagggagaca aaatgacacg aattaaagca gtaaaacaaa aggccatttt
    24001 agatgtggct gaaagtctgg gttattcctt cagacgttta tcaggacaca tttatgaaca
    24061 cccagaccat gattcctttc ggatttttgc cgataccaat actttcaaat ggttttcaag
    24121 agatatacaa ggggatgtaa ttgactttgt tcaattagta gcaggcgtta ctttcaaaga
    24181 ggctgtatct tatcttgaaa ctggagattt tgaacaagct aagttgatag aagaaactta
    24241 tcaaccattt caatactatt tgcatgaaga accctttcag caagcacgta cttacttgca
    24301 agtcgtccgt ggcctaagtg atcagactat taataccttt ggcagacaag gattgcttgc
    24361 tcacgctact tatcaatcgg agcccgtatt agtgttaaaa agctatgacc acaacgggac
    24421 cttacaggcc gcaagccttc aaggtctcgt caaaaatgaa gaaaaacacg atcgaggtta
    24481 tctcaaaaaa atcatgaaag gatctcatgg ctatgtcggt attagtttcg atattgggaa
    24541 tcctaagcga ctcatttttt gtgaatcagt tatcgatatg atgagttatt atcagcttta
    24601 ccaaaagcaa ttatccgatg ttcgcctgat ttcaatggaa ggtttaaaac tttctgtgat
    24661 tgcttatcag accttgcgtc tagcagcaga ggaacagggg aaattggcat ttctagatac
    24721 agtaaatcca agcagactta gtcattatct tcaggcaata caagagacga caaccttctt
    24781 ccaaactcat tcaaatgcat taacattggc tgttgataac gatgaggcag gaagagaatt
    24841 ttgtcagaaa ctgtcagata aaggacttcc gctttctcaa gatttaccac cattgcaagg
    24901 acttgaaaca aagttagatt ggaatgatat tgtgaaacga caaaaggaat tatctttaag
    24961 agatcttatc cagtcagccc aaacaaaagt cattagagat catcctccac ctaaacgaga
    25021 gcacactttt gatgtgataa caaaatcaag tccgctatca taatctagaa tgtgacaagg
    25081 aggacaccct atgagtgtga ttgaacgtct ggctgaaaaa gtagctagac aagaagaaaa
    25141 ggtctcacgt gagacggaga aattggaaca ctatcgtgac caactacaaa cagctatgta
    25201 cagtaccttt atcaaacggc aacaatctag tcagttgtca tttcatgaag cactagagca
    25261 agcctttggt aaagaaacca cactacaccc aaattacaga aatgaggata cagaatgagt
    25321 aaaacatgga attttgatca gccactagat gatgtgaaac caacatcatc ccatgaagaa
    25381 cgagcaaaaa ttgcagcact tttccataaa caagatgaaa aaccaattga agaagtagac
    25441 tatgtggctg cttttgaaca gcaacaaaag gagtcagaat ctaaagatgt acagacgcac
    25501 gaatcaaaag ttaaacaaag ccagccgaag aaagtaaata tcacaagcga ctataaacaa
    25561 cacttggcag acactattgc acaaaacaac aaggatattt cagcctgtca aaagcaaatt
    25621 gaagaacttc atcaattgat tgatgaaaag aaaatccaaa ataaaaagtt gcaggctatt
    25681 tcagtagcca ttgatgattt ataagtcagg tagtcctatg cgggctacct ttttataatt
    25741 gaaggagaag ataatgaaat tttttcaaaa gaaaaaacag aaccaggatc aatttaaacg
    25801 actgattcat cgactatcag agatgtcaga tagtgaacta accaaagtag agcaactcct
    25861 agatgtggtc tttgattcaa gtttcaagtc tagtgagaga gtggaaatat ctagggacat
    25921 tataaccgat gaggaacaaa ttcttgataa gtcaatccaa gaagcgaaaa ataaactgaa
    25981 tactgagcag ttggagaagc gaatagagga gtttaagcaa tcgaagaaac ctaaaaacgg
    26041 ataatgcacc aaatatggtg cgttatccgt tttttatggt ataatgaaag caaatatgag
    26101 gaggtcttta tctatgatcg gagacaatat aaaatcacta cgccgaacac acgatttaac
    26161 acagccagaa tttgcgaaaa tggttgggat ttcacgcaat agcctgagtc gctacgaaaa
    26221 tggtaccagc actgtttcaa ctgaactcat cgaccgtatt tgtcagaaat ttaacgtttc
    26281 ttatgtcgat attgtaggag aggacaagat gttaacacct gtcgaagatt accatctgac
    26341 tttaaaagta gaagtgataa aagaacgtgg agcagccatt ttatcaaagc tttatagata
    26401 ccaggatagt caagatattg tttttgatga tgaatctaat ccttggattt tgatgagtga
    26461 tgacttggcc gaattgatca atacgaaaat ttaccttgtc gctactttcg atgaaataga
    26521 gcgttacaat ggctatttag atggtataga gcggatgtta gacatggtgc atcatcgggt
    26581 ggtggcttaa tgagactgga agaatttagt gaggctgagt ttcagaaggc cttgcagcga
    26641 accattcggg ccttaacccg aggaaagacg attccagatc aaccgaaagc tattttgctt
    26701 ggtggacaaa gcggagcagg taagacgact attcatcgta tcaagcaaaa ggaatttcaa
    26761 ggtaatatca ttattatcga tggtgacagc taccgttctc agcatcccaa ttacttagcc
    26821 ctgcaagaaa agtatggcaa ggatagcgtg gactatacca agggatttgc aggaaaaatg
    26881 gtagaacagt tggttgatga actcagtacg caaggctacc atttgctgat tgagggaacc
    26941 ttgcgaacta ctcaagttcc tcgtcagact gctcaattat tatcttctaa aggttaccaa
    27001 gtttccttag cagtgattgg taccaaacca gagctttctt atctcagcac cttgattcgt
    27061 tacgaagagc tttatgccat cgatccgact caggctagag caacaccaaa agaacaccac
    27121 gatgggattg ttgagaactt ggttgataac ttgagggagt tagaaagtga aaatctcttt
    27181 gaccagattc aaatttatca aagagataga acttgtatct atgattctga aactgctgaa
    27241 ggttccgcag cagaagtgct acaagaatgc ctatttggaa aatggagcaa ggtagaggag
    27301 gagatggtga aggagagtga actcctactt aaggaattac ttgagaaaaa taaataattg
    27361 attttaaaga taatattttt ttgggggtta gaattgcgag gatttaataa taagataaaa
    27421 tctgtttatc gagaattaac aaataccaaa gagaaattcg g
//
LOCUS       DBNFCK010000011        27418 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.011, whole genome shotgun sequence.
ACCESSION   DBNFCK010000011 DBNFCK010000000
VERSION     DBNFCK010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 27418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27418
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.011"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..813
                     /gene="murQ"
                     /locus_tag="AC1PIW_000390"
     CDS             <1..813
                     /gene="murQ"
                     /locus_tag="AC1PIW_000390"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HIP6605354.1"
                     /translation="DGLVALAVQRILPQINAVIEIVVDCLKKDGRLIYVGAGTSGRLG
                     VLDASECPPTFGVSPELVQGLIAGGNGAVRAAKEGAEDNRAAAVEDLQQIGISAKDVV
                     IGLAASGRTPYVLEAVTYAREIGAKTASISCVQDAEISPLVDAPIEVLVGPEVVTGSS
                     RMKAGTAQKMILNMISTTSMIQLGKVFRGFMVDVQPTNQKLIQRAKRIICETTGCTEM
                     EAEHIFQQANQDVKLAILMQLSQLDQIEAKKLLENYHNHISFAIQSILDKEN"
     gene            815..2242
                     /locus_tag="AC1PIW_000391"
     CDS             815..2242
                     /locus_tag="AC1PIW_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HIP6605355.1"
                     /translation="MKNTKDVAKQVLENVGGRENILGNAVCMTRLRLQLRDDKLVHLD
                     SLKNIEGVLGVVEADTLQIVFGPGTVTKVGQAFSELTGISLGSVEEDYTTADVAATNK
                     RINKEKYDKPVQRFLQKIANIFIPILPGIIAAGLINGITNVINVSTGNAFAMEWWYQC
                     IRTMSWGLFTFLPIYVGMNAAKEFKGSPILGAMAGAMSVAVPSMPLLTVVDDKSILLP
                     FVDKAFSPSAGGLLAALFVGIFFAFLERNIRKYMPSFLDTFLTPLCTVILGGLVALLV
                     IQPLGEVLTTAIYSGLNFVYEKLGIVGGYILSAGFLPLVSVGLHQALTPIHVLLNDPS
                     GPTGGINYLLPILMMAGGGQVGAGLALFLKTKNKKLKQLTRDSIPVGILGIGEPMMYA
                     VTLPLGKPFLTACLGSGFGGILAVLFHLGTVSQGVSGLFGLLIMVPGSQLQFVIAMLA
                     AYVGGFVLTWFFGVDEDRIETVYGS"
     gene            2232..3050
                     /locus_tag="AC1PIW_000392"
     CDS             2232..3050
                     /locus_tag="AC1PIW_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HIP6605356.1"
                     /translation="MEVNDFFSRFGFSLYLSNSFANSEKWFIEYQNAGFSFVFSSLNI
                     NEEEKGDEKIRHIVTKCQREGLTLIVDINQDSLKRYGNEGIKKLGIKALRIDDGITIE
                     VMKSLSEDFLLVLNASTLSDDFLRKMMDVGIEKTKVLACHNYYPKPYTGLGGSSVRDL
                     NSRLHSCGIKVITFIPGDNKRFPLFEGLPTVEEHRALPPIQAALDCLMCCESDYVCIG
                     DNALSSASLERFSYLTKGIIALSAQFPKQLKGMNFENRIDAVNMSYVLAIQGLS"
     gene            3062..3262
                     /locus_tag="AC1PIW_000393"
     CDS             3062..3262
                     /locus_tag="AC1PIW_000393"
                     /inference="COORDINATES: protein motif:HMM:NF017701.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar glycosidase domain-containing
                     protein"
                     /protein_id="HIP6605357.1"
                     /translation="MQGPASHRKRGDIVLANEQFLRYQSELEICLVDLPQDDRQMVVG
                     HVHESDVPLLDFVKHPFHFTFI"
     gene            complement(3345..3872)
                     /locus_tag="AC1PIW_000394"
     CDS             complement(3345..3872)
                     /locus_tag="AC1PIW_000394"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6605358.1"
                     /translation="METCHLVVPSLKWKNQILAYKEAFSNEHLHGGGKLQQFEAVEEW
                     LEHIKAVSSYGTNHTERSPSSTFLCIREKDQQMVGICTIRHDLNHEHLKNYIGHIGYS
                     IHPEERRKGYATEQLRIALLEAKKLGIAQVLITAADWNIASQKTILANGGVYEDTRID
                     PNSSERMLRYWIENL"
     gene            4074..6293
                     /gene="pnp"
                     /locus_tag="AC1PIW_000395"
     CDS             4074..6293
                     /gene="pnp"
                     /locus_tag="AC1PIW_000395"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIP6605359.1"
                     /translation="MSKQVFETVFAGKKLVVETGQVAKQANGAVVVRYGDSTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGWMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDPDASAPMAAMFGSSLALAISDIPFNGPIAGVQVGYVNGELI
                     INPDQAQQEASLLELTVAGNKDAINMVESGAKELSEEVMLEALLKGHAAIQELLDFQN
                     QIVAAVGKEKADVELLQVDPELQAEIVAAYNDDLKKAVQVEEKLAREDATNAVRETVI
                     ATYEEKYAEHEEFDRIMRDVHEILELMEHTEVRRLITEDKVRPDGRRVDEIRPLDAEV
                     DFLPNVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDDEYKKRFLHHYNFPQYSVGS
                     TGRYGAPGRREIGHGALGERALEQVLPSLEDFPYAIRLVAEVLESNGSSSQASITAGT
                     LALMAGGVPIKAPVAGIAMGLISDGTNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIDGITPQILEEALAQAKKARFEILDVIEATIPEVRPDLAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEDGLVAIFSPDRAAIERTKEIIAGLVREAKV
                     DEVFQAKVVRLEKFGAFVNLFDKTDALVHVSEMAWTRVNKPEDLVEVGDVVDVKVIKI
                     DDKGRIDASMKALLPKPEGYVEPEKRERSEKPRRHKEHKEKKDNNFGEFKFHKVDKK"
     gene            6349..6966
                     /gene="cysE"
                     /locus_tag="AC1PIW_000396"
     CDS             6349..6966
                     /gene="cysE"
                     /locus_tag="AC1PIW_000396"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HIP6605360.1"
                     /translation="MGWWKDTIEIVKEKDPAARNSLEVLLTYPGVKALAAHRLSHWMW
                     QNGFKLLARMHSQFWRFWTNIEIHPGAEIASGVFIDHGAGLVIGETAIVESGVMLYHG
                     VTLGGTGKDTGKRHPTVRKGALVSAHAQVIGPVEIGENAKVGAAAVVLADVPADVTVV
                     GMPAKIVRVHGQKDEKIIHDMEDGREHYTTKLAELREASHRSSHL"
     gene            6981..7754
                     /locus_tag="AC1PIW_000397"
     CDS             6981..7754
                     /locus_tag="AC1PIW_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HIP6605361.1"
                     /translation="MLLEEFENTSAVIEPTDKAIRGGGEVCETMIFSFNGEIVDRVSQ
                     LAESREGGHLQTLNGRHPWYILERDGLQVAVMLAVIGAPMAVGHLEELKACGFENFIV
                     LGSCGVLDKSLAADKIILPSSALRDEGTGYHYAPASEEISYDPALLLTMEKALDQAGI
                     EHVRAKTWTTDAFFRETAAKVKRRLAAGAMVVDMEASAIMAWANFRQAKVYQFFYTAD
                     YVDHHKNEWDARREERMADSMTFFEVAMAIARKLESRSC"
     gene            7944..9287
                     /gene="cysS"
                     /locus_tag="AC1PIW_000398"
     CDS             7944..9287
                     /gene="cysS"
                     /locus_tag="AC1PIW_000398"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIP6605362.1"
                     /translation="MIKIYDTMTRSLRDFVPLEEGKVKMYVCGPTVYNYIHIGNARSV
                     VAFDTIRRYFEYRGYDVTYISNFTDVDDKIIKAANEAGMTTKELSDKFIAAFKEDVTK
                     LGVKPATKNPRVIDYMDEIIDFVQVLIDKGYAYEAAGDVYFRVEKVENYARLANKTLS
                     DLEVGASGRVEGEGQLKEHPFDFALWKTAKSGEVSWGSPWGAGRPGWHIECSVMATEI
                     LGATIDIHGGGADLEFPHHTNEIAQSECKTGQTFANYWMHNAMLNINDEKMSKSLGNF
                     LTAHDMLEQIDGQVLRFFLATQHYRRPLNYTEKAISDAEINLKYLKNTYTQPVQNSVD
                     KSALELHLAAFEAAMDDDFNAANGITAIFDFAKWINSGNYDETVKEAFGKMLAVFGIV
                     FEEEVLDSEIEALIAERQAARANRDFATADRIRDELAAQGIKLLDTKDGVRWTRD"
     gene            9280..9678
                     /locus_tag="AC1PIW_000399"
     CDS             9280..9678
                     /locus_tag="AC1PIW_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HIP6605363.1"
                     /translation="MTKAVDVNLINGIALAFEGDAVYSMYIRRHLIFKGLTKPNKLHG
                     EANKYVSAKAQAGLISALLEAQLLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGF
                     EAVLGYLHMTEQMERLDELVGWCIKFVENN"
     gene            9924..14399
                     /locus_tag="AC1PIW_000400"
     CDS             9924..14399
                     /locus_tag="AC1PIW_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033925 - mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HIP6605364.1"
                     /translation="MTKCDFDKRQRFGIRKFTIGACSVLIGATLFGTQVSADQLENVD
                     SLSEPAVELSSVVNEHVEKTEAEEGMSPISADEKAVTEELNGEASVSDSREEKLPSAP
                     SDNPENKLEDTEEAEDKEVDSSDSGLLLSDFPQVEDQGIRPKEIKFDNWDQVLAWEPG
                     ARPDDDLNRASVELVERFRGHVVNERANENAKVEALSNTNSKAKDHASVGGEEFKAYA
                     FDYWQYIDSMVFWEGLIPSPDVIDAGHRNGVPVLGTIFYNWSSSIEDQEKFVSSMRQD
                     PDGSFPVARKLVDMAKYYGFDGYFINQETTGGLVQPLGETMRNFMLYTKEYAESIGYP
                     ITYSWYDAMTYEYGRYHEDGLGEYNYPFMEKEGDKVPADHFFANFNWTRAKNNYSIEV
                     AKRIGRNPFDIFAGFELQRGGSYKTQINWDALFDENGKLKLSLGLFAPDTITSLGATG
                     EDYHENENIFFTGYQGDPTKQKPSDKNWYGLANLIADKTAITSSNFNSSFNTGHGKKW
                     FVDGKVAKDGEWNYRSVSGILPTWRWWIEASGEKLAAAYDFDDAYNGGNSLRFTGDLE
                     KDGKQEIKLYSTKIPLSETSRLRVAHKGGQGANISLALALEPDYQFANKEAWRLLKLS
                     GDWQDQTFDLSSLAGKTVYGIQVVVENEAALSDFDFRLGQLAIYDQENSPTAPKDGQV
                     LAKRLKNAQDAEAVISFTGTDDADYYEVYAQVDGQWKLLTGSSNTRIYLPQLVRSAQA
                     EGRTQALKVHAVGKNGLRSEAGEFIFDWEMTVKDTSLPKPSAENIVLGAEVIGSSFAK
                     KEGGEGIEGMLNGTITSLSDKWSSHQLSGHVDIRLTQPRTVVRWAMDHAGAGGESVND
                     GLMNTKDFDLYYKNENGDWVLAKEVRGNRDHVTDIVLDKPIRAQEWRLDVLTSDNGTP
                     WKAIRIYNWRMYEELDMETPNIPMTHAVARHLGNHQIQVGFKDVPANRKIALYSSPDA
                     VKPLAELETTESGNLIFDPIRFESLPEFIYYRTLEEGKDWSNLLAIRVPQGEKVVKAM
                     EWIAPLEKKVYRQGSPLSLAGAQFRVVFEGDWPAERVNTTNPAVTVRGFDPQKLGEQT
                     LTVSYLGETLAQPLMVYVVEDIAQGEKKAVGLEIQQLPKVQYVVGDDLDVTNGRFAII
                     YDDESTQSFSLTEAGVEIVGFDSRKEGRQSLGLRYAGLETSFDVLVSPKPIVNDEYLK
                     QKIAEIEALQTQTAYLYSSEQTQLALQTALEEAKSVVADTNRTVEQVEAAQTLLEKQL
                     GELDGEKLYQADLGRLNQLIQEVKGIDRPINSSELSSLLEKTEKILGSDSIRPDEMKE
                     LLSEWTDLFEQSSLVTHVGTRDSMESKNLLAPEKPRMDLTYELVPRQIITQTSTDLPL
                     GKERIVQEGADGQMMIAHLVYSDGRRELYSRIVADESQPKIVEVGSGLPIQQEKTLMV
                     ENSLQVEKQIRDASLQKSGLPQTGDTYQEKYVFLGLIGVALAGLSQLAKLRKQGE"
     gene            complement(14451..16580)
                     /locus_tag="AC1PIW_000401"
     CDS             complement(14451..16580)
                     /locus_tag="AC1PIW_000401"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HIP6605365.1"
                     /translation="MYTSIVSTIDTRYGTYNSHAFSNGNTLPLIGVPFGMNYFAPQTS
                     DQTGNWWFNPYEPVYQGIRLTHQPSPWLGDFASLLLTPVTGELSRDSLFHRQSSYQLD
                     QAIFRPDLLQLQSNRYRLISRLAPSRYGASFEIASFANQPIGMVFYSSGKSIYRLLNP
                     HQLLIQIFQVSACQSPDFCMYALLEFEEEITECYHLVKDRKIAKTEVVGEDLHLRINF
                     SSTSIKGRLATSFISPEQAAFLLKDVPDFLQAQKQAENTWNQLLGRIQIRDKKTEDIN
                     FFYHCLYRCLLYPQTFYEPNPDGQAIHFDPISNQAKLGKFFTNNGYWDTFRSSYPLLS
                     LIYPDYLEEFLHGILQHYRDTGFLPKWLSPDERGIMPGTLVDGVIADAACKGLVPDLM
                     EDFLQAMIETSQKTDADQRYGRHGSQIYRQLGYLPLDFNESVSHSLDYAYSDWAISQV
                     AEKLRQDQLAKQYAQMCLGYRQLFDSQTGLMRAKDRQKQFRADFSPLSWGRDYAECSA
                     LQATLSVFHDIDGLIELMGGKEAFTNYLIQLCNQEPYFSPEQYGYEIHEMSEMAQVNL
                     GQLAISNQPSFHIPYLFQWSTKPEYTSLLIHKIRQTAFKTGFQAYPGDEDNGSLSAWY
                     IFSCLGFYPVCPGKADYQIGLPHFKEVDLLLADQHKLTISTPKSDANFQFVKQVSLDK
                     QVVQTIKHDQLIKSNCLEFDLSILPSN"
     gene            16827..18113
                     /locus_tag="AC1PIW_000402"
     CDS             16827..18113
                     /locus_tag="AC1PIW_000402"
                     /EC_number="3.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HIP6605366.1"
                     /translation="MFYTKEIIENWLTGIQKKTADHPSWGSVFERCYTDTLDRTISQL
                     EDGTTFVLTGDIPAMWLRDSTAQVKPYLALARKDEKLRQMIVGLVERQMAFILMDPYA
                     NAFNQAANGRGHQTDHTQIGPWIWERKYEVDSLCYPLQLAYLLWKATGETSHFNQTFF
                     GAAEKIVQLWKLEQHHENSPYSFERDTDRLEDTLTHQGKGAPCAYTGMTWSGFRPSDD
                     ACIYPYLIPSNMFAVVVLGYLGEIAEQFASEEFSDLAEGARRLAQEIDQGIRTFGLTK
                     NQAGETIFAYEVDGLGNQSIMDDPNVPSLLAAPYLGYCQKDDPIYLATRRTILSQENP
                     YYYEGDYAAGLGSSHTFYRYIWPIALAIQGLTTDDKEEKARLLDTMVACDGGTGLMHE
                     SFHVDDPTLYSREWFSWANMMFCELVLDYYDIRPEE"
     gene            18115..20787
                     /locus_tag="AC1PIW_000403"
     CDS             18115..20787
                     /locus_tag="AC1PIW_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HIP6605367.1"
                     /translation="MGKETVHIISHSHWDREWYLPLEEHRMRLVELFDDLFDLFETDP
                     EFRSFHLDGQTIVLDDYLEIRPQNRELLKKYIQDGRLIIGPFYILQDDYLITSEANMR
                     NTLLGHLESKKWGRAPKIGYYPDTFGNMGQAPQLLRQAGIDVALFGRGVKPVGFDNQV
                     LGDDQFQSTFSEMIWEGADGSQVLGILFANWYSNGNEIPVDEEEARVFWEKKLADVRK
                     YASTSHYLLMNGCDHQPVQKNLSQALRLARKLYPDIDFVHSSFEEYIAAVKEELPVDL
                     SRVKGELISQETDGWYTLANTASSRIYLKQANDQASQLLEQVVEPLVVMTGDKVPRDE
                     LRYAWKLLLQNAPHDSICGCSVDQVHSEMETRFKKVKQVAHFLSQRSLDRWEKQVDSS
                     QLDGLLFTVFNTTACRQTQLVEVDLLVEQEDFRDGQLEQHFQSMAAISLPALGLFTSQ
                     GQEVEATIEDLGVNFRYDLPKDAFRSANFARQIRVRFVVENQAPFSWQTYQVKPIGDT
                     RVNSSLSEQFENDYYRINVMDGQLLLEDKRTGQAYKDWLRFEDCGDLGNEYIFMAPKN
                     DQPIYGQIEYFGLVYQTEAVSIAKVMHRLQLPVAMETSLEDEQKRLVEFKHRTSQRSQ
                     EKKDLLLTTYLTLYRHQSQIRIQTDFHNQIKDHRLRAVFDIGIEAENHLADSIFEVVE
                     RPNQPHPAWENPSNPQRHRSFISLSDGRQAMTVSSKGLHEYEILDKGKIALTLLRATG
                     ELGDWGYFPTPEAQCLRECQLDYAFEICPVEEGFASFQRAQAFQGHLLTKQLTSHSGH
                     LPCDHQFLSHPALEEDRLCVTSLKVAETSDRILLRYYNMSQEQLAGWSEWTEGVDLLE
                     EPLEDLPARIGSQAIRTEVVGHHGKEKEHGSSLF"
     gene            20768..21634
                     /locus_tag="AC1PIW_000404"
     CDS             20768..21634
                     /locus_tag="AC1PIW_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIP6605368.1"
                     /translation="MVVACFDIGGTGIKAALIGSDKQINYRQEWKTPSSLAELLALMD
                     SVIQPVDGLEAISLSIPGAVDLETGVIQGLSAVPYIHGVSWYELLSQYGCPVYLENDA
                     NCVGLSELAVDDQLSTFACVVCGTGIGGALIVDRKLIRGKQGFGGEFGYMLIATGKGP
                     LKNWSQLASTGSLVRQVASQYEEAVEDWNGKRIFELAEQGDEVCQLAIEEMTRNLAYG
                     ILNISYFLEPEVLAIGGSISQNPVFIQAIQEQLEAIQKEYPEEFPYLPAIRACHYQQD
                     ANLMGAYMKTLQ"
     gene            21644..23527
                     /locus_tag="AC1PIW_000405"
     CDS             21644..23527
                     /locus_tag="AC1PIW_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HIP6605369.1"
                     /translation="MMLQINHSLRQSEGVLEEICSSLGLDCQLELEVGGKESLRIEGS
                     AGSYRIRAPKEHMIYRGLLVLASQLAQGETDIQILERPAYRQLGFMEDCSRNAVLTVE
                     ASKILIRQLALIGYSHFQLYMEDTYQLRDEPYFGYFRGAYTKEELQAIEEECHRYGME
                     FIPCIQTLAHLIAYLKWNISSIQAIRDVDNILLIGDERTYALIDKMFEALSHLKTRTI
                     NIGMDEAHLVGLGQYLNQHGYQKRSLIMCQHLERVLDIADKYGFHCSMWSDMFFQLLT
                     ASKDYTGQLEIDSEIQAYLDRLKDRVTLIYWDYYQTSRESYGQKLASHQQLGDQIAFA
                     SGAWKWIGFTPDNDFSMRIAPKAHAACQEYGVQEVTVTAWGDNGGECATFSILPSLHA
                     WAELQYRGNLGCLAEHFYQLHQVSLDDFLQLDLPNKTPSHPGPGHHGFNPSRYILYQD
                     ILCPLLQEHIDAEKDNAFYQELAPRLAEIGSRAKGYAYLFDTQAKLCQVLATKAAISV
                     GIRQAYQEGNRQVLAEKVDGLQQLRIDLESFYQALSYQWMVEKKVFGLDTVDIRLGGL
                     DTRIRRAIQRLQAYLNNEVPKLDELEVPILPYDDFHQDKGFIATTANQWQIIATASTI
                     YTT"
     gene            23729..24658
                     /locus_tag="AC1PIW_000406"
     CDS             23729..24658
                     /locus_tag="AC1PIW_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6605370.1"
                     /translation="MKDLIKNLKQNAIFFLMVLPGVIWLILFFYIPVFGNVIAFKDYR
                     HTGNGFWDSLIQSKWIGLDNFKFLFQSSDAYIITRNTILYNLGFIVLGLIFAVGIAII
                     FSEMRSKKLVKIYQTTTLFPYFLSWVIISFFVYAFLSPDKGLINNILKGMGMDPINWY
                     STPTYWPFILLFLGIWKGLGYNSIIYYATIMGIDPTYYEAAIVDGATKWQRIRYVTLP
                     QLMPLMTILTILAVGNIFRADFGLFYQIPKNSGPLFNVTQVLDTYVYRGIAGTGDIGM
                     AIAAGLYQSVVGCILVVVTNLIVRRFDKESALF"
     gene            24668..25591
                     /locus_tag="AC1PIW_000407"
     CDS             24668..25591
                     /locus_tag="AC1PIW_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIP6605371.1"
                     /translation="MKKKKQITSVSIHSFSKKTDLFFSSLLGIFALSCTLPFIFVIII
                     SMTDETSLAINGYSFWPEQWSLAGYEYLFQLKDQILQALFITFFVTIVGTSLNVFFTV
                     LYAYAISRNSFRYRRFFTLMALFTMLFSAGTIPTYIVMTSFLNLKDTVAALILPMLLS
                     PFNIMVMRSFFRKTISESIIESARIDGASEFRIFFQICLPLTLPGIATIALFTALAFW
                     NDWYNALLYIQSDNLFPLQYLLMKIQANIQYLSENAAAAAAMSSEVAGALPKEATRMA
                     IVVVSTLPIACLYPFFQRYFVKGLTIGGVKE"
     gene            25661..27142
                     /locus_tag="AC1PIW_000408"
     CDS             25661..27142
                     /locus_tag="AC1PIW_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIP6605372.1"
                     /translation="MKTWKKFAYSCVTLASAAALAACGSLTGNKTASSSTTTEDGLTN
                     ILMYQIGDAPKNIDVLLENANKIIEKEAGAHLDIKYISWGDYKDKMAIITSSGEEYDI
                     AFADNYIINAQKGAYADLTELYKKEGKALYDIIDPAYIKGNTIDGKIYAVPVIGNVAS
                     QQMLSFNQDMVDKYGFDISKVDSYDDVESMLATIKEKEADVTPFAWVKSSKPSTDGLE
                     YPAGNELPFAIDLKGDTTKVVNPFEVDRHMDNLRTIHKYYKAGYIMADAATTDATFSL
                     DVANWFVREETQGPADFGDSLLSTVAGYKITSKPITSAYKTSGSTQVANFVISKTSKN
                     KEKAMEVLTLINTNPELLNGLVFGPEGVNWEKDPSAENRIKLLDGYKGDTRMSAWNTG
                     NSQILYITDKVTDEDVKKAEELLAGAEESPLLGFNFNADAVKTEITSIQNIMSQYAAS
                     INTGTVDPDVAVPEMLKKLEAEESYKKVKEEMQKQYDEFLANK"
BASE COUNT         7821 a         5300 c         6702 g         7595 t
ORIGIN      
        1 gatggcctgg ttgctttagc tgtacaacgt atactgcccc aaattaatgc agtaatagaa
       61 atagtcgttg actgcttgaa aaaggatggt agactgatct atgtcggagc gggaacctca
      121 ggaaggctag gagttttgga tgcttcggag tgtccgccga cttttggagt ttctccagag
      181 ttagtacaag gacttattgc tggaggaaat ggagctgtac gtgccgccaa agagggggca
      241 gaggataacc gagcagcagc tgtagaggat ctgcaacaaa ttggtatatc ggcgaaggat
      301 gtggttatcg ggcttgctgc ttcaggcagg actccatatg tccttgaggc agtcacatat
      361 gctcgtgaaa ttggagcaaa gacggccagt atttcctgtg tacaagatgc ggaaatctcc
      421 ccattggtag atgctccgat tgaagtattg gttggaccag aggttgtaac gggatctagt
      481 cgaatgaaag ctgggacagc tcaaaaaatg attctgaata tgatttctac cacctcgatg
      541 atacaactag gaaaagtttt tcgaggattc atggtagatg tgcaaccgac caatcaaaaa
      601 ctgattcaac gagcaaaaag aattatttgt gaaacaactg gctgtacaga gatggaggcg
      661 gaacatattt ttcaacaagc caaccaagac gtgaaattag ccatcttgat gcagttgagt
      721 caacttgatc aaatagaggc aaaaaaactt ctggaaaact atcataatca catttcattt
      781 gcaattcaat caattttgga taaggagaat taaaatgaaa aatacgaaag atgttgcaaa
      841 gcaagttctt gaaaatgttg gtggcaggga aaatatccta gggaatgcag tttgtatgac
      901 acgacttcgt ttacagttac gagatgacaa gctggttcac cttgatagtc tcaaaaacat
      961 agaaggggtt ttgggagttg ttgaggcaga tacacttcaa attgtttttg ggcctggaac
     1021 agtgacaaag gttggccaag cattttcaga actgacaggt atatcattgg gaagtgttga
     1081 agaagattac acaacagcgg atgttgctgc tacaaataaa aggataaata aagagaagta
     1141 cgacaagcca gttcaacgat tcttgcaaaa aattgctaat atttttatac ctatattacc
     1201 tggtataatt gcggcaggtt tgataaatgg gattacaaat gtcattaatg tatcaaccgg
     1261 gaatgctttt gcaatggaat ggtggtatca atgtattagg actatgagtt ggggactctt
     1321 tacttttttg ccaatctatg ttggaatgaa tgcggcgaaa gaatttaaag gaagtccgat
     1381 tttgggagca atggccggag ctatgtctgt ggcagttcct tcaatgcctt tgttgacagt
     1441 agtggatgac aaatcaattt tattaccttt tgtggacaag gcattctcac ctagtgcagg
     1501 cggtttgtta gcggctctat ttgttgggat tttctttgca tttctagaaa gaaatatccg
     1561 aaaatatatg ccaagttttt tagacacttt tttgactcct ctttgtacag taatattggg
     1621 tggtctggtt gctttgttag tcattcagcc tcttggagaa gtgttaacca cagctattta
     1681 tagtggatta aattttgtat atgagaagct aggtatagta ggtgggtata ttttgtcagc
     1741 aggcttctta ccattggtat cagttggact acaccaagca ttgactccaa tacatgtttt
     1801 gttgaatgat ccatctgggc caacaggtgg tataaattat ctcttgccta ttttgatgat
     1861 ggcaggtggt ggacaagtcg gggctggttt agcactcttt ttgaaaacaa aaaataaaaa
     1921 gttgaaacaa ctaacacgag attctattcc agtcggaatc ttgggaattg gtgaaccgat
     1981 gatgtacgcc gtcactttgc cattgggcaa acctttccta accgcttgtt taggttcagg
     2041 ttttggcggt attttggcag tgttattcca tttagggact gtatctcaag gagtttctgg
     2101 tttgtttgga ctgttaatca tggtacctgg aagccaactg caatttgtta tagcgatgct
     2161 ggctgcatat gtaggtggtt ttgtactaac ttggttcttt ggtgtggatg aagatagaat
     2221 tgagaccgta tatggaagtt aatgattttt tcagtcgttt cggtttttca ctctatttat
     2281 ctaactcatt tgctaattcg gaaaagtggt ttatagaata tcaaaatgca ggattttcct
     2341 ttgttttctc ttcgcttaat ataaatgaag aagagaaggg agacgaaaag attcgtcata
     2401 ttgtaacaaa atgtcaacga gaagggctga ccttgattgt ggatatcaat caagatagtt
     2461 tgaagcgata tgggaatgaa gggattaaaa agctcggtat taaagcactt aggattgatg
     2521 atggaattac tattgaagtc atgaaatcat tgtcggaaga ctttttactt gtcttaaatg
     2581 catctacatt gtcagatgat tttttaagga agatgatgga cgttggtatt gagaaaacaa
     2641 aagttttggc atgtcataat tattatccta agccttatac aggtttaggg ggaagtagtg
     2701 tccgtgacct aaatagtcgg ctacactctt gtggaataaa agtcatcacc tttattcctg
     2761 gagataacaa gcgttttccg ctttttgaag gacttccaac cgttgaagaa catagagcgt
     2821 tgcctccaat tcaagccgca ctcgattgtt tgatgtgttg tgaaagtgat tatgtttgta
     2881 ttggggataa tgccttatcg agtgcttcat tagaaagatt ttcatatttg acaaagggta
     2941 taatagcctt atcagcgcag tttccaaagc aattgaaggg aatgaatttc gaaaatagaa
     3001 ttgatgcagt gaatatgtca tacgtgctag ccattcaagg gctcagttaa aaggatatgc
     3061 aattcaaggg ccagcaagtc atcgaaaacg aggagatatt gttcttgcta atgaacagtt
     3121 tcttcgttat caatcagaac tagaaatttg tttggtcgac ttgcctcagg acgaccgcca
     3181 aatggttgtt ggccatgtcc atgaaagcga tgtccctttg ttagattttg tgaaacatcc
     3241 atttcatttt acctttatat aaaacgagta tttggtctga gagttcaata atcggtaact
     3301 taaaaaagcc tccaaacgat ttagtatcat tggaggtttt tatattacaa attttcaatc
     3361 caatatcgga gcatacgctc gctactatta gggtcaatac gggtatcctc ataaactcca
     3421 ccatttgcaa ggatggtctt ttgagaggca atattccaat ctgcagcggt gattaagact
     3481 tgggcaattc ccaatttttt agcttccagc agggcaattc gtagttgttc agtcgcatag
     3541 ccttttcttc gctcttctgg gtgaatagaa tagccgatat gaccaatata attctttaaa
     3601 tgctcgtgat taagatcgtg gcgaatagtg caaatgccaa ccatctgttg atctttttca
     3661 cgaatacaaa gaaatgtaga agaaggagaa cgttctgtat gatttgtccc ataagatgac
     3721 acagctttaa tgtgttctag ccattcctca actgcttcaa attgttgcag tttgccacca
     3781 ccatgaagat gttcattgct aaatgcttct ttataggcta ggatttggtt cttccatttt
     3841 aaactaggaa ctacaagatg gcaagtttcc attttctacc tccaactatt ttgaatagtt
     3901 tatcatgagt atgttttctt gtcaaaaata gtttcaggcc tttatttcta cctaaaatat
     3961 gataaaataa ggagtatgcg tggttttgtg caaaagagag tgaaacaagc ttgtttgatt
     4021 gtcttttgta gaaaacacgc aagaaaaatt tcagaaaaaa taggagacaa ttaatgtcaa
     4081 aacaagtatt tgaaacggtt tttgctggca aaaaattggt cgttgaaaca ggtcaagttg
     4141 ccaaacaagc caacggtgcg gttgtcgttc gctacggtga ttccacagtt cttacagcgg
     4201 cagttatgtc aaaaaaaatg gcgacgggag acttcttccc cctccaagtc aactatgaag
     4261 agaaaatgta cgctgctggg aaattcccag gtggctggat gaagcgtgaa ggtcgtccgt
     4321 caactgatgc gaccttgaca gctcgtttga ttgatcgtcc gattcgtcca atgtttgcgg
     4381 aaggtttccg taacgaagtt caagtcatca acacagtgct ttcttatgat ccagatgcat
     4441 ctgctccaat ggcagccatg tttggttcct cattggcatt agcgatctca gacattccat
     4501 tcaatggtcc aattgctggt gttcaggttg gttatgttaa tggcgaattg attattaacc
     4561 cagatcaagc tcaacaagaa gcctcccttt tagaattgac agttgctggt aacaaagacg
     4621 ctatcaacat ggttgaatcg ggtgccaaag aattatcaga agaagtgatg ttggaagcac
     4681 tcttgaaagg tcatgcggcc attcaagaac tcttggattt ccaaaatcaa atcgtggcag
     4741 cagttggtaa ggaaaaagca gatgtggagc ttcttcaagt ggacccagaa ttgcaggctg
     4801 agattgttgc agcttacaat gacgatttga aaaaagcggt gcaggttgaa gaaaaattgg
     4861 cccgtgaaga tgcgaccaat gccgttcgcg agactgtcat tgcaacttac gaagaaaaat
     4921 acgctgagca cgaagaattt gaccgtatca tgcgtgatgt ccatgaaatc ttggaactca
     4981 tggagcatac agaagttcgt cgtttgatta ctgaagacaa ggtccgtcca gatggtcgcc
     5041 gtgttgatga gattcgtcct ttggatgcag aagtagactt cttgccaaat gttcacggct
     5101 caggtctctt tacccgtggt caaacgcaag ccctctctgt cttgaccttg gcaccaatgg
     5161 gagaaaccca aatcatcgac ggtttggatg atgagtacaa gaaacgcttc ttgcatcact
     5221 acaatttccc gcaatactca gttggctcaa ctggtcgtta cggtgcacca ggtcgtcgtg
     5281 agattggtca cggtgctctc ggtgagcgcg ctcttgagca agtcttgcct agcttggaag
     5341 acttccctta cgctatccgc ttggtagcag aagtcttgga gtcaaacggt tcttcttcac
     5401 aggcatctat cacagctggg acccttgccc ttatggcagg tggtgtgcca atcaaggcac
     5461 ccgttgcagg gattgctatg ggtctgatct cagatggtac caattacacc gtcttgaccg
     5521 atattcaagg tcttgaggat cacttcggcg acatggactt caaggtagct ggtactcgtg
     5581 acggtatcac agcccttcaa atggatatta aaattgacgg tatcacacca caaatcttgg
     5641 aagaagcctt ggcacaagct aagaaggcac gttttgaaat ccttgatgtc atcgaagcga
     5701 ctatcccaga agttcgtcct gacttggcac caactgcacc gaagattgac actatcaaga
     5761 ttgatgtgga caagatcaag attgtcatag gtaagggtgg tgaaaccatt gataagatta
     5821 ttgcagaaac tggcgtgaaa attgatatcg acgaagacgg tcttgtggcg atcttctcac
     5881 cagaccgtgc tgcaattgag cgcacaaaag aaatcatcgc tggtcttgtc cgtgaagcaa
     5941 aagtggacga agtcttccaa gcaaaagtgg ttcgtttgga gaaattcggt gcctttgtca
     6001 acctttttga caagaccgat gccctcgtcc acgtttctga aatggcttgg acacgtgtca
     6061 ataaaccaga agatttggtt gaggttggcg atgttgtaga tgttaaggtg ataaaaattg
     6121 atgataaggg acgtattgat gcttctatga aagctcttct accaaaacca gaaggctacg
     6181 tggaaccaga aaaacgtgaa cgttcagaaa aacctcgtcg tcataaagaa cacaaagaga
     6241 aaaaagataa taactttggt gaatttaaat ttcataaagt agataaaaaa taatggtgaa
     6301 gtaggcaagt cccgaaatgg acttgtcttc tcgctagaag gagaagtcat gggttggtgg
     6361 aaggatacca tagaaattgt aaaagaaaaa gatccggcag cacggaactc cttggaagtc
     6421 cttttgacct acccaggagt caaggcactg gctgctcatc gtctctctca ctggatgtgg
     6481 caaaatggtt ttaaattatt agctcggatg cacagtcagt tttggcgttt ttggaccaac
     6541 attgaaattc atccaggtgc agaaattgcc tcgggagtct ttattgatca tggcgcggga
     6601 ttggtaattg gtgagactgc aattgtcgaa tctggtgtaa tgttatacca tggagtaact
     6661 cttggaggaa caggcaagga tacagggaag cgccatccaa cagttcgaaa aggggcactc
     6721 gtttctgctc atgcccaggt gattggccca gttgaaatcg gagaaaatgc gaaagtaggt
     6781 gctgctgctg ttgtcctagc cgatgttcca gcagatgtga cagtggtcgg tatgccggct
     6841 aaaatcgtcc gtgttcatgg tcaaaaagat gagaagatca ttcatgacat ggaagatggt
     6901 cgtgaacact acacgacaaa attagcagag ttgagagaag ccagccatcg ttcgtcgcat
     6961 ttgtaggaaa gaaggctgat atgctactag aagaatttga aaacacctct gcagttattg
     7021 aaccaacgga caaggctatc cgtggtggtg gagaggtatg tgagacgatg attttctcct
     7081 tcaatggaga aatcgtggac cgagtaagtc agctagctga aagcagggaa ggtggtcatc
     7141 ttcagacgtt aaatggtcgc catccttggt atattttgga aagagatgga ctgcaggttg
     7201 cggtcatgtt ggcagtaatc ggtgctccaa tggcagttgg tcatctggaa gagctgaaag
     7261 cctgtggctt tgagaatttt atcgttctgg gttcttgtgg ggtgctggat aagtcgctcg
     7321 ctgcggataa gatcatcctt cctagctctg ctcttcggga cgagggaact ggttaccact
     7381 acgcacctgc tagcgaggaa atcagctatg accctgctct gctattaacc atggaaaagg
     7441 ccttggacca agcgggaatc gagcacgtgc gggctaagac ctggaccacg gacgctttct
     7501 ttcgggaaac agctgccaag gtcaagcgtc gcctagccgc tggtgccatg gtggtcgata
     7561 tggaagcttc tgctatcatg gcctgggcaa actttcgtca ggccaaggtc taccaatttt
     7621 tctacacggc agactatgtg gaccaccaca agaatgaatg ggatgcccgt cgagaggaac
     7681 ggatggctga cagtatgacc ttctttgaag tggccatggc cattgcaaga aaattagaaa
     7741 gtagatcatg ttaaaaaaat tagtaagaca aaactggcct tatgtgttga ccgccatcgc
     7801 tgggacaatc atgtttatcc ttaaattctc ccaaggaagc tggcaggtag ggatgatttg
     7861 gttggccgcg acagcttatt ggctagtaaa attgtatcaa aagtatcaag tcctaaagaa
     7921 tactcagaag taatggagaa actatgatta aaatttacga taccatgact cgcagtctgc
     7981 gtgactttgt gcccttggaa gagggtaagg tcaagatgta tgtttgcggt ccgacggttt
     8041 acaactatat ccatatcggt aatgcccgta gtgtcgtggc ttttgatacc atccgccgct
     8101 attttgaata ccgtggctat gatgtgacct acatctcaaa ctttacggat gtggatgaca
     8161 agattatcaa agctgctaat gaagcaggca tgacaaccaa ggaactgtct gataaattta
     8221 tcgcagcttt caaggaagat gtgacaaaac tgggtgtcaa gccagctacc aaaaatcctc
     8281 gcgttatcga ctacatggat gaaatcatcg attttgtgca ggtcttgatt gacaagggtt
     8341 acgcctatga agcggctggc gatgtttatt ttcgtgtgga aaaggtggag aattatgctc
     8401 gtttggccaa caaaacccta tctgacttgg aggttggggc ttctggtcgt gttgagggag
     8461 aaggtcaatt gaaagaacat cctttcgatt ttgccctgtg gaaaactgcc aagtcaggcg
     8521 aagtttcttg gggaagtcct tggggagcgg gtcgtccagg ttggcatatc gagtgttcgg
     8581 tcatggcgac agagattttg ggagctacca tcgatattca cggtggtggt gcggacttgg
     8641 aatttcctca ccataccaat gaaatcgccc agtctgagtg caaaacaggt caaacctttg
     8701 ccaactactg gatgcacaat gccatgctca acatcaatga tgagaagatg tccaagtcct
     8761 tgggcaattt cctgacagcc catgatatgt tggaacaaat tgatggtcag gtcttgcgct
     8821 tcttcctagc cacccagcac taccgccgtc cactcaacta cactgagaag gctatttcag
     8881 atgcggaaat caatctcaaa tatctaaaaa atacttatac acaacctgtg caaaactctg
     8941 tggataagtc agctcttgaa ttacatctgg cggcctttga agcagccatg gatgatgatt
     9001 tcaacgcagc aaacgggatt acggctatct ttgactttgc caaatggatc aactcaggca
     9061 actacgatga aactgtaaaa gaagcctttg gcaagatgct cgccgtcttt ggtattgttt
     9121 ttgaagaaga agtcttggac agcgagattg aagccttgat agcagaacgt caagcagcgc
     9181 gtgccaatcg ggactttgcg acggctgacc gtattcgtga tgaattggca gctcagggca
     9241 tcaagctctt ggataccaag gatggtgtga ggtggacacg tgactaaggc agtagatgtc
     9301 aatctcatca atggaattgc tctggctttt gaaggggatg ctgtctattc catgtatatc
     9361 agacggcact tgattttcaa gggactgacc aagcctaaca aattgcatgg cgaggctaac
     9421 aaatacgtct cggccaaggc tcaggcaggc ttgatttctg ccctcttaga agctcagcta
     9481 ttgaccgaaa aagaggaaga aatctacaag cgggggcgca ataccaatag ccataccaag
     9541 gccaagaatg cggacgtggt cacctatcgg atgtccacag gctttgaggc ggtcttgggc
     9601 tacctgcaca tgacagagca gatggaacgc ctggatgaac tggtaggttg gtgtattaag
     9661 tttgtagaaa ataattaacg aagacaacga ggagggaaac ttcctctttt ttgttttctc
     9721 taaactaaag ttctttttat ctgatttaaa gaagggaacc atgagggctt gaaggtcata
     9781 tttttcctct gtacaatgga attaataaaa cctgataaac ttcgtgtgat agctgttcta
     9841 tcaaaggtag catgtgccaa ctatgatctg gaaaggagga aattgagaac agctgttgca
     9901 ctacaattgt gtaggaggaa aatatgacaa aatgtgattt cgacaaacgt cagcgattcg
     9961 gtattcgaaa atttacaata ggtgcttgtt ctgttttgat tggtgcgacc ttatttggaa
    10021 cacaggtttc tgcggaccag ctagaaaatg tggacagctt gtcagagcca gctgttgaac
    10081 tctcttccgt agtgaacgaa catgtggaaa agaccgaagc tgaagaagga atgtcaccaa
    10141 tttcagcaga cgagaaagcg gtgaccgaag agttgaatgg agaagcatct gtatcagata
    10201 gtagagaaga aaaactgcct tcagctccgt ctgataatcc agaaaataaa ctagaggata
    10261 cagaggaagc ggaagataaa gaggtagatt cttcagattc tggtctgttg ctttcagatt
    10321 ttcctcaggt agaggatcaa ggaattcgtc cgaaagagat taagtttgac aactgggatc
    10381 aggtgcttgc ttgggagccg ggtgctcgtc cagatgatga tttgaaccgc gcttctgtag
    10441 agttggttga gcgctttaga gggcacgtcg tcaatgagag agccaatgag aatgcaaagg
    10501 tagaagctct ttcaaatacc aactctaagg cgaaagacca tgcctctgtt gggggagaag
    10561 agtttaaagc ctatgccttt gattactggc agtatattga ttcaatggta ttttgggaag
    10621 gcttgattcc tagcccagac gtcattgatg ctggacaccg taatggtgtg cctgttttgg
    10681 gaacgatttt ctacaattgg tccagtagta ttgaagatca agagaagttt gtatcttcta
    10741 tgagacagga tcctgatgga agttttcctg ttgctcgtaa gttggtagat atggctaaat
    10801 actacggttt cgatggttat ttcataaatc aagagactac aggaggcttg gttcaaccac
    10861 taggggaaac aatgagaaac ttcatgctct ataccaagga atatgcagaa tcgattggtt
    10921 atccgataac gtattcttgg tatgatgcca tgacctacga atatggtcgc tatcacgaag
    10981 atggtttggg tgagtacaac tatccgttta tggaaaagga aggagacaag gtccctgcgg
    11041 atcatttctt tgcgaatttt aactggacac gggccaaaaa taactattca atcgaagtgg
    11101 cgaaacgaat cggtcgaaat ccatttgata tttttgctgg atttgaattg caacggggag
    11161 gctcttacaa aacgcaaatt aactgggatg ctctgtttga tgaaaacggg aaattgaaat
    11221 tatcactcgg tctgtttgcg ccagatacca ttacaagttt gggtgcgacg ggtgaggatt
    11281 atcatgagaa tgagaatatc ttctttacag gctatcaagg tgatccaacc aaacagaaac
    11341 cttcggataa aaattggtat ggcttggcca atctcattgc ggataagacg gctattacaa
    11401 gcagtaattt caatagttcc tttaatacag ggcacgggaa aaaatggttt gtagatggca
    11461 aggttgccaa agatggggaa tggaattatc gttctgtgtc tggtatttta ccaacctggc
    11521 ggtggtggat tgaagcaagt ggtgaaaagc ttgctgcggc ttatgatttt gatgatgcct
    11581 ataatggggg caactctctt cgttttacag gcgatttgga aaaagatggt aagcaggaaa
    11641 ttaagttgta ttccaccaag attcctctat cagaaacaag tcgtttacgg gttgcgcaca
    11701 aaggagggca aggtgcaaat atctccctag ctctagcgct tgaaccagat tatcaatttg
    11761 cgaataaaga agcgtggcgc ttgcttaaac tatcaggaga ttggcaagat cagacctttg
    11821 atttatctag tttggcagga aagacagtgt acggtatcca agtggttgtt gaaaatgaag
    11881 cagcgctgtc agattttgat tttcgtttag ggcagttggc tatttatgac caagaaaatt
    11941 cacctactgc accgaaagat ggccaagtcc ttgctaagcg attgaagaat gcccaagatg
    12001 ctgaagctgt gattagcttt acagggacag atgatgcaga ctattatgaa gtatatgcgc
    12061 aggttgacgg acagtggaaa ttgctgacag gttcttctaa tacaaggatt tacctccctc
    12121 aactagttcg ttctgcacag gcagaaggtc gaacccaagc tttgaaagtt catgccgttg
    12181 ggaaaaatgg tttgcgttcg gaagcagggg agtttatctt tgattgggag atgacggtga
    12241 aagatactag ccttcctaaa ccatcggcag aaaacattgt tctcggagca gaagtcatcg
    12301 gttcaagttt tgctaaaaaa gaaggtggag aaggcattga gggcatgttg aatgggacca
    12361 ttaccagttt gtcagataaa tggtcttccc atcaattgag tggccatgta gatattcgtt
    12421 tgacccaacc tcggacagtc gtgcgatggg ctatggatca tgctggtgca ggtggtgaat
    12481 cggtcaatga tggcttaatg aacaccaagg atttcgatct ctattataaa aatgagaatg
    12541 gtgattgggt gttagccaaa gaagttcgtg gcaatcgtga tcatgtgaca gacatcgtat
    12601 tggacaagcc aattcgagcg caggaatggc gtttggatgt cttgacttct gataatggta
    12661 ctccttggaa ggccattcgt atttataatt ggcgtatgta tgaagaattg gacatggaga
    12721 caccaaatat tccgatgacc catgctgttg ctcgccatct cggtaatcat cagattcaag
    12781 taggtttcaa ggatgtccct gccaatcgga aaatagcgct ttattctagt ccagatgccg
    12841 taaaaccact agcagaattg gaaacgacag agtcaggaaa tcttatattc gacccgattc
    12901 gctttgagtc cctgcctgag ttcatttact accgtactct ggaggaagga aaggattgga
    12961 gcaatctatt ggccattcgc gttccgcaag gtgaaaaagt cgtgaaagcg atggaatgga
    13021 tagctcccct agagaaaaaa gtttaccgac aaggtagtcc tttatcgctt gcaggagcac
    13081 aattccgtgt agtttttgaa ggagattggc ctgctgaacg ggtgaatacc accaatccgg
    13141 cagttacagt aaggggattt gatccgcaaa aattaggtga acaaacattg accgtttcct
    13201 atctaggaga aacacttgct cagccactga tggtatatgt tgttgaagat atagcccagg
    13261 gtgagaaaaa agcagtcggt ctagaaatcc aacagttacc aaaagtgcag tatgttgtag
    13321 gagatgattt ggatgtcaca aatggtcgat ttgctattat ctatgacgat gagtcaactc
    13381 agtccttctc tttgacagaa gctggagtag aaattgttgg tttcgattcc cgtaaagagg
    13441 ggcgtcagag cctaggtttg cggtatgctg gtttggagac ttcatttgat gtccttgttt
    13501 caccgaagcc aatcgtcaat gatgagtatc taaagcagaa aatagcagaa attgaggctc
    13561 tacaaaccca aacagcctat ctctattcta gtgagcagac acaacttgcc ttgcaaacag
    13621 ccttggaaga agctaagtct gtggtagcag ataccaatcg gacagtcgag caggttgaag
    13681 ccgctcaaac tcttttagag aaacagttag gagagctgga tggggagaaa ctttaccaag
    13741 cagatcttgg gcgtctcaat cagctaatcc aagaggtgaa aggaatagat agacctataa
    13801 attcttctga gctaagttct ttacttgaaa aaactgagaa aattttgggt tcggatagta
    13861 ttcgtccaga tgagatgaag gagctgctca gtgaatggac tgatttgttt gaacagtcta
    13921 gtctggtaac tcatgtcggt acaagagatt cgatggagtc taagaattta ctagctcctg
    13981 aaaaaccgag aatggatttg acctatgagc tggtgccgag acaaatcatc acacagacca
    14041 gcactgattt gccactgggc aaagagcgaa ttgttcagga gggggctgat ggtcaaatga
    14101 tgatagctca cctagtctat agtgatggcc gtcgcgagct gtattcacga atagttgctg
    14161 atgagagtca gccgaaaata gtggaggtcg gcagcggttt gcctatccag caagagaaga
    14221 ctttaatggt ggaaaatagc ctgcaggttg aaaagcagat cagagatgct tctctgcaga
    14281 agagtggtct tccacaaact ggtgatacct atcaggagaa gtatgtcttt cttgggttaa
    14341 ttggtgtggc tttggctggc ctatctcagt tagctaagct tagaaagcaa ggtgaataaa
    14401 agaaatagtc aggcttggtc ctgactattt cttttgtttg tagaagctgg ttagttactt
    14461 ggtaatatag ataggtcaaa ttctaggcag tttgatttta tcagttggtc atgtttgatg
    14521 gtttggacga cttgtttgtc taatgaaact tgcttgacaa actggaaatt tgcatcgctt
    14581 tttggagtag aaatggtcag cttgtgttga tctgcaagta gcaagtctac ttccttaaaa
    14641 tgcggtagtc cgatttggta gtcagctttt ccgggacaga caggatagaa gcctagacag
    14701 gagaaaatat accaggctga aaggctaccg ttatcctcgt cacctggata ggcttgaaaa
    14761 cctgtcttga aagctgtttg tctgattttg tgaatgagta gactagtgta ttccggtttt
    14821 gtagaccatt ggaaaagata agggatatgg aagctcggtt gatttgaaat agcaagctga
    14881 ccgagattga cttgcgccat ttccgacatt tcgtggattt cgtagccgta ttgttcaggt
    14941 gagaaatagg gctcctgatt acagagctgg attagatagt tggtgaaggc ttcttttccg
    15001 cccatcaatt ctatcagtcc atcgatgtca tgaaagacgg acagggttgc ctgtagggca
    15061 gaacattcgg catagtctct tccccaactt agaggggaaa aatctgctcg gaattgtttc
    15121 tgtctatcct tggcacgcat gagacctgtt tggctatcga ataactgtcg gtagccgagg
    15181 cacatttgag cgtattgctt agccagttgg tcctgcctca gtttttctgc tacttggctg
    15241 atagcccaat cactgtaggc ataatcaaga gaatggctca cagattcgtt gaaatcgaga
    15301 gggagatagc ctaattggcg gtaaatctga ctaccatggc ggccgtaacg ttgatccgca
    15361 tcggtctttt gactggtttc aatcatggct tggagaaaat cttccatcaa atcaggaaca
    15421 agtcccttgc aggccgcatc agcaatgact ccatccacaa gagttcctgg catgataccg
    15481 cgctcgtctg gcgatagcca tttgggtaaa aatccagtat ctcgatagtg ttggagaatg
    15541 ccgtggagaa actcttccaa ataatctggg tagataagtg aaagcagggg ataagaagag
    15601 cggaaggtat cccagtagcc gttgttggtg aaaaacttac ctagcttggc ctgattgctg
    15661 atagggtcga aatgaatggc ttgtccgtca ggattaggtt cgtagaaagt ttgtggatag
    15721 aggaggcaac ggtagaggca gtggtagaag aagttaatgt cctctgtttt tttgtcacga
    15781 atctgaatac gtcctaagag ctgattccag gtgttttctg cttgtttttg ggcttgaagg
    15841 aagtcaggaa catcttttaa tagaaaggcg gcttgctcag ggctgataaa agaggtagcc
    15901 aaacgcccct tgatagaagt gcttgagaag ttgatgcgaa ggtgcaaatc ttctcccacc
    15961 acttccgttt tagctatctt gcgatccttg accagatgat aacattctgt tatttcctct
    16021 tcaaattcta ggagagcata catgcagaaa tcaggggatt ggcaggcgct gacctgaaaa
    16081 atctgaatga gcaattggtg aggattgagc agtcgataaa tggattttcc agatgaatag
    16141 aagaccattc cgataggctg gttagcaaaa gacgctatct caaaactggc tccgtatcgg
    16201 ctgggagcta aacggcttat caagcgatag cgattggatt gtaattgaag gagatcaggt
    16261 cggaaaatag cctgatccag ttggtagcta gattgacggt gaaagaggct atctcggctt
    16321 aattcgcctg tgacaggcgt caataggagg gaggcaaaat ctcccaacca aggactgggt
    16381 tgatgggtta agcgaatgcc ctgatagaca ggttcatagg gattgaacca ccagtttcca
    16441 gtctggtcgc tggtctgggg agcaaaataa ttcatgccga aagggacccc tatcaatggc
    16501 agggtgtttc cgtttgaaaa ggcatgtgag ttataagtac catagcgggt gtcaatggtt
    16561 gaaacaatgg aggtatacat aggctatcct tttctttttt tattgataat aagtggtgtt
    16621 ctccttatta tatagagaat gggaaagaaa ggaaatcgtt ttccgaaact ggcaatattt
    16681 gtgtaaaaat gcgtttaatt tgtgtatagg atttgcatat agaattgtat agactgatac
    16741 taggaacctt gtgtctaatg aaaattatat ggatttgcca gatagaggtt ggaagaactc
    16801 tgagcaaagg ataaaaagga gaaatcatgt tttacacaaa agaaatcata gagaactggc
    16861 tgacaggcat tcagaagaaa acagcagacc acccaagttg ggggagtgta tttgaaaggt
    16921 gttatacgga tacgcttgat aggaccatta gtcagctgga agatgggact acttttgtct
    16981 taacggggga tattccagcc atgtggttaa gggactcgac agcccaggtc aaaccctatt
    17041 tagctttggc tagaaaagat gagaaattac gccagatgat tgtaggtctg gtagagaggc
    17101 aaatggcctt tattttgatg gatccttatg ccaatgcctt taatcaggca gctaatgggc
    17161 gggggcacca gaccgaccac actcagatag gcccctggat ttgggagcga aaatatgagg
    17221 tggattccct ctgctatccc cttcaattag cctatctact ttggaaagcg acaggggaaa
    17281 ccagtcattt taaccagacc ttctttgggg cagctgaaaa aattgttcaa ctctggaaat
    17341 tggagcagca ccatgaaaac tccccttata gttttgaacg ggatacagac cggctagagg
    17401 ataccctcac gcaccaagga aaaggggctc cctgcgccta tacagggatg acttggtctg
    17461 gttttcgtcc aagcgatgac gcctgtatct acccttacct catcccctcc aacatgtttg
    17521 cggtcgtcgt cttgggttat ttaggagaaa tagcagagca gtttgcaagt gaggaattct
    17581 cagatttggc tgaaggtgcc agacggttgg cgcaggaaat tgaccagggg attcggacct
    17641 tcgggctgac caagaatcag gcgggagaaa ctatttttgc ctatgaagtg gacggattgg
    17701 gcaatcagtc tatcatggat gacccaaatg tcccgagttt gctggcggct ccctatctag
    17761 gatactgtca gaaagatgat cccatctatt tggcaacccg tcggactatt ttaagtcaag
    17821 aaaatcccta ttactacgag ggagactatg ctgcgggtct tggttccagt cataccttct
    17881 atcgctatat ctggccgatt gccttggcta ttcaaggatt gacaacggat gacaaggaag
    17941 aaaaagcgcg cttgctggat accatggtag cctgtgatgg cggtactggg ctgatgcacg
    18001 agagtttcca tgtggatgat ccgacgcttt actctagaga gtggttctcc tgggcaaata
    18061 tgatgttttg cgaattggtt ttagattatt atgatatacg accggaggaa taaaatggga
    18121 aaagaaacgg ttcatattat ttcacatagc cactgggata gggagtggta tcttcctttg
    18181 gaagaacatc ggatgcgctt agtagagcta tttgatgacc tgtttgactt atttgaaaca
    18241 gatcctgagt ttaggagttt tcatctagat ggacagacca ttgtcctaga tgactatctg
    18301 gaaattcgtc cgcagaatcg agagttattg aagaaatata ttcaagacgg tcggctgatt
    18361 attggtccct tttacatctt gcaggatgat tacctcatca ccagcgaggc caatatgcga
    18421 aatacccttc tgggtcattt ggaaagcaag aagtggggac gggctccgaa gattggttat
    18481 tatccagata ctttcggcaa tatgggacag gctcctcagc tactgcgtca ggctggtata
    18541 gatgtggcct tgtttggtcg tggggtcaaa ccagttggct ttgataatca ggttctggga
    18601 gatgaccaat tccagtccac tttctcggag atgatttggg agggggcaga tggcagtcag
    18661 gttctgggca ttttatttgc taactggtac agcaatggca atgaaatccc agttgatgag
    18721 gaggaagcac gtgttttctg ggagaaaaaa ttggcagatg ttcgcaaata cgcttcaacc
    18781 tcccactatc tgctgatgaa tggctgtgac caccagcctg tccagaaaaa tctcagccaa
    18841 gcattacgac tggcccgaaa actgtatcca gatatagact ttgttcattc aagctttgaa
    18901 gaatatatcg cagctgtcaa ggaagagtta ccagtagacc tcagtcgtgt caagggagaa
    18961 ttgattagcc aggaaacgga tggctggtac accttggcaa atacggcttc ttcgagaatt
    19021 tatctcaagc aggccaatga ccaagccagt cagctcttgg aacaagttgt agaacccttg
    19081 gtggtaatga caggagataa ggtgcccaga gatgagttgc gctatgcctg gaaactcttg
    19141 ttgcagaatg cccctcatga tagcatctgt ggttgtagcg tagaccaggt gcatagtgag
    19201 atggaaaccc gctttaagaa ggtcaagcaa gtcgctcatt tcttgagcca gcgctcccta
    19261 gacaggtggg aaaagcaggt ggattctagc caattagacg gcttactatt tacggttttc
    19321 aatactacag cctgccgaca aacccagtta gtggaggttg atttgcttgt tgaacaggag
    19381 gatttccgag atgggcagtt agaacaacac tttcaatcca tggcagctat ttctctgcca
    19441 gccttgggcc tctttaccag tcaaggccaa gaggtagaag ctaccattga agatctaggt
    19501 gtcaacttcc gctacgattt accaaaagat gcttttcgct ctgccaattt tgcccgccag
    19561 attcgtgtcc gctttgtagt ggaaaatcaa gcaccatttt catggcagac ctatcaagtc
    19621 aagccgattg gtgacactag ggtgaatagc agtttaagtg aacagttcga gaatgattat
    19681 taccgtatca atgtgatgga tggtcagctc ttgctagaag ataagagaac gggtcaagcc
    19741 tataaagact ggctacggtt tgaagattgt ggagatttgg gcaatgaata tatcttcatg
    19801 gcgcctaaga atgaccaacc gatctatggt cagatagagt acttcggtct tgtctaccaa
    19861 acagaagcgg tcagcattgc caaggtcatg caccgtctgc agctgcctgt agctatggaa
    19921 acaagtctgg aagatgaaca gaagcgcctg gtagaattta aacatcgaac atctcaaagg
    19981 agtcaggaaa agaaagacct gctccttacc acctatctga cactctaccg ccaccaatcc
    20041 caaattcgca tccagaccga tttccacaac caaatcaagg accatcgctt gcgagcagtc
    20101 tttgatattg gaatagaagc tgagaaccac ttggcggatt ccatctttga agtcgttgaa
    20161 cgtccaaatc agccacatcc agcctgggaa aatccgtcca atcctcagcg tcatcgcagt
    20221 tttattagtc tatcagatgg tcgacaagcc atgaccgttt cgagtaaggg cctacatgag
    20281 tatgaaatcc tagacaaggg aaaaatagct cttaccttgc ttcgtgcgac tggagagctg
    20341 ggagattggg gctacttccc aacaccagaa gcccagtgtt tgagggaatg tcagttggac
    20401 tatgcctttg aaatttgccc agtagaggaa ggttttgcca gcttccaaag agcgcaggcc
    20461 ttccaaggac acttgctaac taaacagttg actagccata gtgggcatct gccgtgtgat
    20521 catcagtttt tgagccatcc agcactggaa gaggaccgct tatgcgtgac ttctttgaag
    20581 gtggcagaga cttcggaccg cattctgctc cgttactaca atatgagtca ggagcagtta
    20641 gcaggttgga gcgagtggac tgagggggtt gatttgctag aagaaccact agaggatttg
    20701 ccggctagga ttgggtcaca ggcaatccga acagaggtcg ttggtcatca tggaaaggag
    20761 aaagaacatg gtagtagcct gttttgacat tggtggtacg ggaataaagg cggccctgat
    20821 tggttcagat aagcagataa actataggca ggaatggaaa acgccatcta gtttagcgga
    20881 attgttggcg cttatggaca gtgttattca gccggtagac ggcttggagg ctatttccct
    20941 cagtatacct ggagcggtag accttgaaac aggtgtcatc caggggctga gtgccgttcc
    21001 ttatatccat ggtgtatctt ggtatgagct attaagtcag tatggctgtc ctgtttattt
    21061 ggaaaatgat gccaattgtg tcggtctgag tgaattggct gttgatgacc agttatcgac
    21121 ttttgcctgt gtcgtctgtg gtacgggtat cggtggtgcc ttgattgtgg atagaaaact
    21181 catccgaggg aaacaaggtt tcggtggcga atttggctat atgctgattg ctactggaaa
    21241 gggacctcta aaaaactggt cccaacttgc ttctactgga agtttggtca gacaggttgc
    21301 tagtcagtat gaggaagctg tggaagattg gaacggcaag cggatttttg agctagcaga
    21361 acagggagat gaggtatgtc aattagccat tgaagaaatg acacgcaatc tggcctatgg
    21421 cattttaaat atctcctatt tcctggaacc agaagtgctg gctataggtg gctctatcag
    21481 ccagaatcca gtctttatcc aagctattca ggagcaacta gaggctattc aaaaggaata
    21541 tccagaagaa tttccttatc tacctgccat tcgtgcctgc cactaccagc aggatgccaa
    21601 tctcatgggt gcttatatga agaccctaca ataaggagaa atgatgatgt tacagataaa
    21661 ccattcgtta cggcagtcgg agggggtctt ggaagagatt tgttcctcgc ttggcttgga
    21721 ttgccagtta gaacttgaag tcggtggcaa ggagagtttg cggattgaag gttcagcagg
    21781 ttcttaccgt atccgtgcgc ctaaagaaca catgatttat cgaggcttgc ttgttttggc
    21841 tagtcagttg gctcagggtg agaccgacat acagattcta gaaagacctg cctatcgtca
    21901 actcggtttt atggaagatt gttctcgaaa tgctgtcctg acggttgaag ccagtaaaat
    21961 cttgattaga caattggcct tgataggcta cagccatttc cagctttaca tggaagatac
    22021 ctaccaacta cgtgatgaac cctattttgg ttatttccga ggggcctata caaaagaaga
    22081 attgcaggct attgaggagg agtgccaccg ttacggtatg gaatttattc cctgtatcca
    22141 gaccttggcc catctgatcg cctacctcaa atggaacatt tcatctattc aggccattcg
    22201 agatgtggat aatattctct tgattgggga tgagaggacc tatgccttga ttgataagat
    22261 gtttgaggct ttgtcccatc tgaaaacacg gaccattaac atcggtatgg atgaggctca
    22321 cttggtcggg ctgggtcaat acctcaatca acacggctat cagaaacgaa gtctgattat
    22381 gtgccaacac ttggaacggg tcttggatat tgctgacaag tacggcttcc attgtagtat
    22441 gtggagcgat atgttcttcc agttgttgac agcgagtaag gattatactg gtcagttgga
    22501 aattgattcg gaaattcagg cttatttgga tcgtctcaag gacagagtga ccttgattta
    22561 ttgggattac tatcagacta gtcgtgagag ctatggtcaa aaactagcta gtcatcagca
    22621 gttgggagat cagattgcct ttgcaagtgg tgcctggaag tggattggtt ttactcctga
    22681 taatgatttc agtatgcgga ttgcaccaaa agcacatgct gcctgccaag agtatggcgt
    22741 ccaagaagtg acggtgaccg cttggggaga taatggtgga gaatgcgcaa ccttttcgat
    22801 cttgccgagt cttcatgcct gggcagaact ccagtaccga ggaaatttgg gctgtctggc
    22861 tgagcatttc tatcaactac accaagtttc cctagatgac ttcctgcaat tggatttgcc
    22921 aaataagaca cctagtcacc caggtccagg tcatcacggt ttcaatccca gtcgctacat
    22981 tttgtatcag gatattctct gccccttgct gcaagagcat attgatgccg aaaaagacaa
    23041 tgccttttat caggaattag cgcctagatt ggcagagatt ggaagccgag ccaagggcta
    23101 tgcctatctc tttgatactc aggctaagtt gtgtcaggtt ttagcgacca aggcggctat
    23161 ttcagtaggt attcgtcagg cctatcagga aggaaatcgt caggtcttgg cagagaaagt
    23221 tgacggtttg cagcaactaa gaatagatct ggaaagcttc tatcaggcac ttagctacca
    23281 atggatggta gagaagaagg tgtttggtct ggatacggtg gacattcgcc tagggggatt
    23341 ggatacccgc attcgccgag caatccagcg gttgcaagcc tatctgaaca atgaagtacc
    23401 taaactagat gaactagaag tgccaattct cccttacgat gacttccatc aggacaaggg
    23461 atttattgcg acaactgcca accaatggca gattattgcg acggcttcaa ccatctatac
    23521 gacctagtag ttctcgagct cgaaagggct caatttttta tgcaaatttt gattagcaac
    23581 ccagattttc tttgtttttt acagaaggta tatcggttgt tatatacacc tataatttgt
    23641 agaattttgc ctataatgtg gcaattcaca gttggcccaa tcaggagtaa actaataatg
    23701 taaaggttca taatagaaag gagctatgat gaaagacctc ataaaaaatc taaaacaaaa
    23761 tgccattttt ttcctaatgg tattgccagg agtcatctgg ctcattctat ttttctatat
    23821 tccagtattt ggcaatgtga ttgcttttaa ggactatcgc catacaggta atggcttttg
    23881 ggatagtctc atacaaagta agtggatagg attggataat tttaaatttc tatttcaatc
    23941 atccgatgcc tatatcatca caagaaatac catcctatat aatcttggtt ttatcgtact
    24001 cggactgatt tttgcagtag gtattgccat tatctttagt gagatgcggt cgaagaagct
    24061 ggtaaagatc tatcagacca caaccttgtt cccttatttc ctttcttggg tcattatcag
    24121 tttcttcgtt tacgcatttt tgagtccaga taaaggattg ataaacaata ttctcaaagg
    24181 aatggggatg gatcccatca actggtacag cacaccaacc tattggccct tcatcttgct
    24241 tttcttagga atttggaaag gtttgggcta taacagtatt atttattatg caaccattat
    24301 gggcattgac ccaacctact atgaagctgc gattgtggat ggagcgacca aatggcaacg
    24361 catccgctat gtcacccttc ctcagttaat gcctctgatg actattttaa cgattctggc
    24421 tgtcggaaat atcttccgag cagactttgg ccttttctat caaattccga aaaattcagg
    24481 tcctctcttc aatgtgactc aggtccttga tacctacgtt tatcgtggaa ttgcaggaac
    24541 aggggacatc ggtatggcta tcgcggcagg tctctatcag tctgttgtcg gctgtatctt
    24601 ggtggtagtg accaacctga ttgttcgtcg ctttgataag gaatcagcct tattctagga
    24661 ggggagtatg aaaaaaaaga aacaaatcac ttctgtttct attcattcat ttagtaagaa
    24721 aacagatcta tttttctcaa gtttactagg gatatttgcc ctatcttgta cccttccctt
    24781 catatttgtt atcatcattt ctatgacaga tgagacaagc ttggctatca acggctattc
    24841 tttctggcca gagcaatgga gtttggcagg ttatgaatat ctctttcaat taaaagatca
    24901 gattctccaa gccctcttta tcaccttctt tgtgaccatt gtagggacga gcctgaatgt
    24961 cttcttcacc gttctttacg cctatgccat ttcgagaaac agtttcagat acagacgatt
    25021 ttttaccctg atggctctct ttaccatgct gtttagcgca gggaccattc caacctatat
    25081 cgttatgacc tcgtttttaa atctcaaaga tacagtggca gccttgattc tacctatgct
    25141 gctatctcct ttcaatatca tggtgatgag gtccttcttt agaaagacta tttctgagtc
    25201 tattatcgaa tctgccagga ttgacggggc cagtgaattt cggattttct tccaaatctg
    25261 cttgccacta accctaccag gtatcgcaac aatcgctctc tttacagccc tcgctttttg
    25321 gaatgactgg tacaacgctc tcctctatat ccagagcgat aacctcttcc ctctccaata
    25381 cctgctgatg aaaattcaag caaacattca atatctctct gaaaatgctg ccgcagcagc
    25441 ggccatgtcc tcagaagttg caggggccct tcccaaagaa gcaacacgta tggctattgt
    25501 ggtcgtatcg actctaccaa ttgcctgtct ctatcctttc ttccaacgtt actttgtcaa
    25561 aggtttgaca attggcggag tcaaggagta gagacatcac tctatcttaa caatcgtatt
    25621 ccatacttca ttatctaaaa aaatagaaag gaacagccaa atgaaaacat ggaaaaaatt
    25681 tgcctactcc tgcgtgacac ttgcgtccgc agctgccctt gccgcttgtg gtagtctgac
    25741 tggaaataag accgcatcaa gtagcactac aacagaagat ggattgacaa acatcctcat
    25801 gtatcaaatc ggagacgcac cgaaaaacat cgatgttctt ttggaaaatg ccaataagat
    25861 tatcgaaaaa gaagcagggg cccatctaga catcaagtat atctcatggg gggactataa
    25921 agataaaatg gccattatta cctcatctgg tgaggaatat gatattgcct ttgcagacaa
    25981 ctatattatc aatgcgcaaa aaggagctta tgctgattta acagaacttt acaagaaaga
    26041 aggcaaggcc ctctatgaca ttattgaccc tgcctatatc aaaggaaata ccatcgacgg
    26101 aaaaatctac gcagtccctg tcatcggtaa cgtagctagt caacagatgc tttccttcaa
    26161 ccaagacatg gttgataagt atggttttga catttctaaa gtagatagct acgatgatgt
    26221 ggaaagcatg ttggcaacca tcaaagaaaa agaagcagat gtaacaccat ttgcctgggt
    26281 aaaatctagc aaaccctcaa cagacggctt ggaatatcca gctgggaatg agttaccatt
    26341 tgctatcgac ttgaaaggcg acactacaaa agtggtaaac ccatttgaag tggaccgtca
    26401 catggataac cttcgtacca ttcacaaata ctataaggct ggctacatta tggcagatgc
    26461 agcgactact gacgcaacct tctcacttga tgtagcaaac tggttcgttc gtgaagaaac
    26521 acaaggacca gctgactttg gcgatagcct cctttcaaca gttgcaggct ataagattac
    26581 ttctaaacca atcacatctg cctataagac aagcggatct acccaagtag caaactttgt
    26641 catttcaaaa acatctaaga acaaagaaaa agctatggaa gtattgaccc ttatcaatac
    26701 aaatccagaa ttgctgaacg gcttggtatt tggtccagag ggagtcaact gggaaaaaga
    26761 cccaagtgct gaaaatcgta tcaagttgtt ggatggttac aaaggcgata ctcgtatgtc
    26821 agcttggaac acaggtaaca gccaaattct ctacatcact gacaaggtaa cagatgaaga
    26881 tgtgaaaaaa gcagaagaat tgctggcagg tgcagaagag tctccacttc ttggcttcaa
    26941 cttcaatgct gatgcagtta agactgaaat tacaagtatc caaaatatca tgagccaata
    27001 tgcagcaagt atcaatactg gtactgtgga tcctgatgtt gctgttccag aaatgctgaa
    27061 aaaattggaa gcagaagaat cctacaaaaa agtaaaagaa gaaatgcaaa aacaatacga
    27121 tgagttttta gcaaataaat aatacgatac aggtgatgaa gtgtgaaaag agggagtggg
    27181 aaagaactcg actggtctaa aaaaagttcg tcaacaattt ttcgttttaa gttgttgagc
    27241 tgaaacagtc tatccccata ctgtttcact cccacccccg cacagctcaa actgtctggg
    27301 agacagtttg aggttggaga tgaagcgaat tctgttcgca tcagtcgtat aggtcggatt
    27361 tggagtgtaa aacacgaaca aatctgccaa tcaaccactg cgatgagatg tcgacacg
//
LOCUS       DBNFCK010000012        26876 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.012, whole genome shotgun sequence.
ACCESSION   DBNFCK010000012 DBNFCK010000000
VERSION     DBNFCK010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 26876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26876
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.012"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..285)
                     /locus_tag="AC1PIW_000409"
     CDS             complement(<1..285)
                     /locus_tag="AC1PIW_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HIP6605373.1"
                     /translation="MRVVFGIDVSKASSEVAILVNGEKVHNYTMSNDAIGFSRLLGDL
                     KTVHKPEIIFEATGVYSRRLQAFLDEHGYAYTRLNPLEAKKQLDSLRVRKT"
     gene            619..984
                     /locus_tag="AC1PIW_000410"
     CDS             619..984
                     /locus_tag="AC1PIW_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HIP6605374.1"
                     /translation="MTVKINTKDGQIELTDDVIATVVGGATTEIFGVVGMASKSAIKD
                     NFQALLRKENYSKGVVIKTLEDGRIAVDVYTVMSYGVKISEVSRNIQERVKFNLENQL
                     GISVDAVNVFIQNIKVVGE"
     gene            987..2651
                     /locus_tag="AC1PIW_000411"
     CDS             987..2651
                     /locus_tag="AC1PIW_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HIP6605375.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTITNGAKEVADKSASTVGEVAQILSKGLLMGARGNSGVITSQLFRGFGQSVKGKVE
                     LDGKDLAQAFQHGVEVAYKAVMKPVEGTILTVSRGAATAALKKAEETDDAVEVMRATL
                     DGANRALKKTPEMLPVLKEVGVVDSGGQGLVYIYEGFLSALTGEYIASEDFEATPAVM
                     SEMINAEHHKSVAGHVATEDITFGYCTEIMVALRQGPTYVKDFDYEEFRNYLNDLGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQAGLQYGSLVKVKVDNMREQHEAQVEKVESFQKPA
                     EQKEYAIIAVAAGEGLTEIFKSQGVDYVISGGQTMNPSTEDFVNAIELVNARNVILLP
                     NNKNILMAAQTAAEVAEVAVKVVETKTLPQGFTSLLAFDPSRDLESNFEAMTASLAEV
                     KSGSVTTAVRDTTIDGLEIHENDNLGMVDGKIVVSNPDMMETLVATFDKMLDEDSEIV
                     TIYIGEDGNEELAQTLAEQLEEQFEDVEVEIHQGNQPVYPYLFSVE"
     gene            2752..3192
                     /gene="tsaE"
                     /locus_tag="AC1PIW_000412"
     CDS             2752..3192
                     /gene="tsaE"
                     /locus_tag="AC1PIW_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIP6605376.1"
                     /translation="MYSQNENELIAIGERIGKACKPNQVLVLSGDLGAGKTTLTKGLA
                     KGLKIEQMIKSPTYTIVREYEGAMPLYHLDVYRIGDDPDSIDLDDFLYGGGLTVIEWG
                     ELLDVSLFDDYLLIRIEKEGDGRRLTVESHGAQSSDLAKEFQNV"
     gene            3185..3712
                     /locus_tag="AC1PIW_000413"
     CDS             3185..3712
                     /locus_tag="AC1PIW_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HIP6605377.1"
                     /translation="MSEKEVYFSEAEPADAAAFIDFMNQVARETDYLVMDETGFRFSV
                     DEIETIFDAGIENPRELCLLAKVGSEVVGAISVKSSKQFRISHIGNIFIAVKKAYWGH
                     GIGTILLEEVLHWAEEMDVLKRLELTVQVRNQAAVHLYQKLGFNIEGTQIGGARTDEG
                     EWLDLYYMGKLIGEV"
     gene            3709..4902
                     /locus_tag="AC1PIW_000414"
     CDS             3709..4902
                     /locus_tag="AC1PIW_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIP6605378.1"
                     /translation="MTIGKKIFLMSLAIIGLTVGAGLIYGASLLNFSTDAISKTFKQL
                     DGEEKITPIDATEPLTILLMGVDMDQATRGGDWEGGRSDSMILVTVNPKTKETNMMSL
                     TRDIMVEIAEANGESSGTVEKLNHSYSYGQAPMAIATIEKMMDINIDRYIEINMDGLV
                     ELVDAVGGIEVNNTLGFPISISEHEPAYTSIVQPGKQLVNGDQALVYARMRYDDPEGD
                     IGRQRRQREVITAIIKKLLQLDGFTQYKKILTAISNNMRTNIEISPVTIPNLLGYKDS
                     VSKLNSYQLRGVDQMVDAIYYQLPTSTHLLEMQNVLKKSIGLEEKTDLVTNVKVYEGQ
                     MGLPSPINVYDAYTNLLLLEASPWAESLDPEIGSPSATTTVTTEEQVTDFSTEPVLGD
                     ENGAQ"
     gene            complement(5011..5313)
                     /locus_tag="AC1PIW_000415"
     CDS             complement(5011..5313)
                     /locus_tag="AC1PIW_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIP6605379.1"
                     /translation="MKLRNIILAVSAATASFLAVTHRDKITKEIKETKQLLSDIQLSK
                     DNIQEQLAIIQSFQEPLQEMASDLQYKTRVYQQSIAGNLEEIQQILKKYQNENNTP"
     gene            complement(5315..5728)
                     /locus_tag="AC1PIW_000416"
     CDS             complement(5315..5728)
                     /locus_tag="AC1PIW_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIP6605380.1"
                     /translation="MSDCIFCKIISGEIPASKVYEDDQVLAFLDITQVTKGHTLVVPK
                     KHYRNVLDMDEEAAATLFSVVPTIARQLKEKLRASGLNIVNNNEEAAGQTVFHTHIHL
                     LPRFNNQDGLSIQFKANAPDFPALAQLAQELYLGE"
     gene            5791..6525
                     /locus_tag="AC1PIW_000417"
     CDS             5791..6525
                     /locus_tag="AC1PIW_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6605381.1"
                     /translation="MLEVKNVTGGYVNIPVLKDVSFTVEDGQLVGLIGLNGAGKSTTI
                     KEIIGLLTPYQGQIVIDGLTLLQGAENYRKKIGFIPETPSLYEELTLKEHLEVVAMAY
                     DLSWEQAWGRVEHLLKLFRLDEKLDWFPVNFSKGMKQKVMIICAFMVEPSLLIVDEPF
                     LGLDPVAISDLIALLGEEKSKGTSILMSTHVLDSAEKMCDSFVILHQGQVRATGNLEE
                     LQSAFGMDGASLNEIYLALTQEGVAG"
     gene            6522..7562
                     /locus_tag="AC1PIW_000418"
     CDS             6522..7562
                     /locus_tag="AC1PIW_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6605382.1"
                     /translation="MKELFQQRRLDFLNRCSKYLRYVLNDHFVLVLMVLLGFLSLQYR
                     QLLLSFPENPLPIVLVVIAISLLVLLSGRIATYLEEADQIFLLTKEKDILQGLEVAAR
                     RTVLFWSSIQVVVQLVLLPIYLKLGLPVWMIVSGLLTLLVLKCLFVKRQLVAYQSQGV
                     LDWSKAIQDEQKRKQSILRFFALFTTVKGIGTTVKPRSYLNGILRLVKSRQTWFYLYL
                     RAFLRSGDYLGLTIRLLLLAILSLFAIEESWLSIGLVLVFHYLLVFQLLGLYKHYDYQ
                     YITQLYPLEKDSKKIGLQNLLRVILYSLLVVELGFALLFSKENAMVVVLLLVGIFLHE
                     IYLPLKLKKLID"
     gene            7612..8406
                     /gene="ccrZ"
                     /locus_tag="AC1PIW_000419"
     CDS             7612..8406
                     /gene="ccrZ"
                     /locus_tag="AC1PIW_000419"
                     /EC_number="2.7.1.15"
                     /EC_number="2.7.1.229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0051726 - regulation of cell cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell cycle regulator CcrZ"
                     /protein_id="HIP6605383.1"
                     /translation="MQFDTSGLQLQSIAGNSGKAFKGLRSDGTPVFVKYEMPPIVSAL
                     AREQITPPVLSANREVGMGHRVEQEWLNGRTLERQDMTSKQIRQILVRMHYSRILLNQ
                     ALQMNYTYMEPQDLVYRWQKEAPTRLGQNSYLQSICQDLLNNLPRFRQEVATFVHGDL
                     HHSNWVETTSGLVYLTDWETACVTDRMLDVAYILTHYIPRQSWEEWLRAYGYKYNSTV
                     LNKVYWYGQLGYLNQIAKHVESYNVLAANKEIYALRQFRQSFYKEV"
     gene            8403..9041
                     /gene="trmB"
                     /locus_tag="AC1PIW_000420"
     CDS             8403..9041
                     /gene="trmB"
                     /locus_tag="AC1PIW_000420"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HIP6605384.1"
                     /translation="MRVRNRKGASELLANNPQYVISNPEECKGKWAEIFGNNNPIHIE
                     VGSGKGRFVTGMAAQNPDINYIGIDIQMTVLSYALDRVLEAGLPNIKLLQVDGSSLTN
                     YFAPAEIDQLYLNFSDPWPKKRHEKRRLTYKSFLDTYKEILPEKGEIHFKTDNRGLFE
                     YSLASFSQYGMVLKQVWLDLHADGLEGNVMTEYEEKFSNKGQVIYRVEAQFQ"
     gene            9612..10082
                     /gene="rimP"
                     /locus_tag="AC1PIW_000421"
     CDS             9612..10082
                     /gene="rimP"
                     /locus_tag="AC1PIW_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HIP6605385.1"
                     /translation="MSSIIELVTAAITPAIQTPYELVDVEYGKMGGDYVLSIFVDKEG
                     GISLQDTADLSEKISPILDTIKPDPFPDQYMLEVTSPGLERPLKTADAVEKAVGKYIH
                     VKLYQAIDKIKVFEGTLLSFDGTDLIMEYMDKTRKKEVTIPYQTVAKARLAVKL"
     gene            10156..11298
                     /gene="nusA"
                     /locus_tag="AC1PIW_000422"
     CDS             10156..11298
                     /gene="nusA"
                     /locus_tag="AC1PIW_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIP6605386.1"
                     /translation="MSKEMLEAFRILEEDMGINKADIIDAVTESLRSAYKRRYGQSES
                     AVIEFDEKKGDFHVFTVREVVDEVFDSRLEISLKDALAISSAYEMGDKIKFQEDPAEF
                     GRVAAQSAKQTIMEKMRKQKRAITFNTYKQHENEIMSGTVERFDNRFIYVNLGTIEAQ
                     LSKQDQIPGEVFQSHDRIEVYVYKVEDNGRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGSNIKKITSKFHPARYDAKNDR
                     MIPTEENIDVIEWVADEAEFIYNALAPAEVDQVLFDTEDGKHATVVVPDDKLSLAIGR
                     RGQNVRLAAHLTGFRIDIKSASEYEAFEATQYATDEVVEEVAEEQE"
     gene            11315..11611
                     /gene="rnpM"
                     /locus_tag="AC1PIW_000423"
                     /gene_synonym="ylxR"
     CDS             11315..11611
                     /gene="rnpM"
                     /locus_tag="AC1PIW_000423"
                     /gene_synonym="ylxR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase P modulator RnpM"
                     /protein_id="HIP6605387.1"
                     /translation="MAKARKIPLRKSVVSGEIIDKRDLLRIVKNKEGQVFIDPTGKAN
                     GRGAYIKLDNDEASQAKKRRVFDRSFSMEVAEEFYDELIAYVDHKIKRRELGLE"
     gene            11568..11906
                     /locus_tag="AC1PIW_000424"
     CDS             11568..11906
                     /locus_tag="AC1PIW_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HIP6605388.1"
                     /translation="MLIIRSRGENLDSNKRQQILNLLGLAQRAGRLVSGEDLVVEAIQ
                     KGQAKLVFLAEDAAGNLSKKVTDKSHTYQVEVVTVFSTLELSAAVGKARKVLAVTDAG
                     FTKKMRSIME"
     gene            11924..14746
                     /gene="infB"
                     /locus_tag="AC1PIW_000425"
     CDS             11924..14746
                     /gene="infB"
                     /locus_tag="AC1PIW_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIP6605389.1"
                     /translation="MSKKRLNEIARELGVSSKEVVAKAQELGFEVKSHASSVDEASAK
                     RLAESFGGQKSEATKVAAKVSKPEKVDETPKVETAKVEKAKETQPVVKEEVAASAVQS
                     ASHRPQSRNFKAEREARAKEQAAKRAQNQGKGGQAKSDQDRRDNRQLGQGRSNNERND
                     RRDNRRDQRPEERKDNRFGDRRDNRDNRRQDNRSGRLARFEQREAAKPAGPKIDFKAR
                     AAALKAEQNAEYARTSEERFRQAQEAKKQPKKPKEIKFEEPVVESKPFVKPALVASVP
                     EQVAETTVDTRRKKQARPDKKRDFNSDEEDGPRKQQRNRNSQNQVRNQRTSNWNNNKK
                     NKKGKANQPAKPVTERKFHELPTEFEYTAGMTVAEIAKRIKREPAEIVKKLFLMGVMA
                     TQNQSLDGDTIELLMVDYGIEAKEKVEVDNADIERFFVEEGYLNEEEMTERPPVVTIM
                     GHVDHGKTTLLDTLRNSRVATGEAGGITQHIGAYQIEEAGKKITFLDTPGHAAFTSMR
                     ARGASVTDLTILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGE
                     LAEHGVISTAWGGESEFVEISAKFNQNIDELLETVLLVAEIQELKADPTVRAIGTVIE
                     AHLDKGKGAVATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKTAGPSTPVSIT
                     GLNETPMAGDHFAVYEDEKSARAAGEERAKRALLKQRQATQRVSLENLFDTLKAGEVK
                     SVNVIIKADVQGSVEALASSLQKIEVEGVRVNIVHSAVGAINESDITLAEASNALVIG
                     FNVRPTAEARSQAEADDVEVRLHSIIYKVIEEMEDAMKGMLDPEYEEKIIGEAIIRET
                     FKVSKVGTIGGFMVVRGKVTRDSSVRVIRDGVVVFDGKLASLKRYKDDVKEVGNAQEG
                     GLMIENYNDLKVDDTIEAYIMEEIKK"
     gene            14902..15255
                     /gene="rbfA"
                     /locus_tag="AC1PIW_000426"
     CDS             14902..15255
                     /gene="rbfA"
                     /locus_tag="AC1PIW_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIP6605390.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMVGD
                     LSTAKVYYTIMSNLASDNQKAQTGLEKATGTIKRELGRKLTLYKIPDLVFEKDQSIEY
                     GNKIDQMLRALDQKD"
     gene            15537..16208
                     /locus_tag="AC1PIW_000427"
     CDS             15537..16208
                     /locus_tag="AC1PIW_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HIP6605391.1"
                     /translation="MKIFISKLMLVSTVVLLAACQSNQVKGGESSSSSASSQVSDGSS
                     LQQASTIASSSETQASSVTTPELSEVYQSVIDRYQANMGQPAEAINQDEASSYLSLLT
                     SQGQEYSGIFYSLYDVNHDGTEELLLALDKSGEYVLIDLYTQLADESLRLVDNFRNLG
                     FEIGPDALLRPLQDGTYLFEGAGVFRIYQYNAMIPGLKRISESDTNPETSPLLELKSL
                     HWVKL"
     gene            16373..17527
                     /locus_tag="AC1PIW_000428"
     CDS             16373..17527
                     /locus_tag="AC1PIW_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HIP6605392.1"
                     /translation="MKTNLKKLLYSGVTLMSLGVLAACSSTSSSTTTSSSAATSQTSV
                     ATTSNNSTSSDSSSSTIDWSALPTTEVKLSNDGLKITEGGTYILTGSTTAGVTVETDA
                     NVRIILAGAEISSSDTAAINVINADNVELEIKDGTTNTVKDTSNHTDTNIEGAIHVEA
                     DLTITGNGSLTVEGNFQDGIVSTDDLVVNSGNITVTAVDDGIRGKDSLTINGGTINVT
                     AGGDGIKSTNDTDTTKGYTTITGGEITVTAGDDGIKAETALTIDGGTITVSESAEALE
                     GTNITINGGTLDVYGSDDAINAASTTSSEIFIKVTGGDLKVAVGSGDTDAFDANGDIY
                     ISGGTIDVTAQSAFDFDGTAELTGGTVTVNGEQITQITATGPGAGGHGGW"
     gene            complement(17563..18222)
                     /locus_tag="AC1PIW_000429"
     CDS             complement(17563..18222)
                     /locus_tag="AC1PIW_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HIP6605393.1"
                     /translation="MEFDLFLMICNYIGTIAFAVSGAVKGFKKKLDIFGISLLGIITA
                     VGGGIIRDTMANRIPAALTDPTAIYLSLGVAISMYLIVINLKQDKPLDKKMIHFLSQT
                     NLIFDAIGLAIFALIGASTGISLQLNALTSGILAALTGVGGGITRDLLVNETPIVLKE
                     DVYAVLAFFSGVLYHLCVVNWKLPQIPTFITIFTISLIIRLLVIKYKINLPNMESKRK
                     V"
     gene            18455..19423
                     /locus_tag="AC1PIW_000430"
     CDS             18455..19423
                     /locus_tag="AC1PIW_000430"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIP6605394.1"
                     /translation="MVSITKEQHLDMFLKMQQIRDVDMKLNKLVRRGFVQGMTHFSVG
                     EEAAAVGPIAGLTDEDIIFSHHRGHGHVIAKGIDINGMMAELAGKATGTSKGRGGSMH
                     LANVEKGNFGSNGIVGGGYALAVGAALTQQYLGTDNIVIAFSGDSATNEGSFHESMNL
                     AAVWNLPVIFFITNNRYGISTDISYSTKIPHLYQRAAAYGIPGHYVEDGNDVIAVYEK
                     MQEVIEYVRAGKGPAMVEVESYRWFGHSTADAGVYRTKEEVNEWKAKDPLKKYRKYLT
                     ENKIATDEELDAIEAQVAEQVEASVKFAQESPDPDISVAYEDVFVD"
     gene            19710..20705
                     /locus_tag="AC1PIW_000431"
     CDS             19710..20705
                     /locus_tag="AC1PIW_000431"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIP6605395.1"
                     /translation="MAETKVMALREAINLAQSEEMRKDEKVFLMGEDVGIYGGDFGTS
                     VGMLDEFGPKRVRDTPISEAAIAGSAVGAAQTGLRPIVDLTFMDFITIALDAIVNQAA
                     KTNYMFGGGLKTPVTFRVASGSGIGSAAQHSQSLEAWLTHIPGIKVVAPGTANDAKGL
                     LKSSILDNNPVIFLEPKALYGKKEEVNLDPDFYIPLGKGEIKREGTDVTIISYGRMLE
                     RALKAAEEVAAEGISVEVVDPRTLIPLDKELIIESVKKTGKVILVNDAYKTGGFIGEI
                     ASIITESEAFDYLDAPIIRIASDDVPVPYANILENAVLPNVEKIKAAIYKQVNKG"
     gene            21046..22434
                     /locus_tag="AC1PIW_000432"
     CDS             21046..22434
                     /locus_tag="AC1PIW_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002885106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HIP6605396.1"
                     /translation="MAIEIIMPKLGVDMQEGEIIEWKKQEGDFVNEGDVILEMMSDKT
                     SMELEAEESGVLLKIVHGNGATVPVTEVIAYIGAEGETVEAGASSAPAVEPAAAIEEV
                     PAGRTPVIVAPATAAKPQGSGKVRATPAARKLARELGIDLGLVPGTGANGRVHKVDVE
                     DFKGAAPKATPLAARIAADQGVDLSTLTGSGVNGKIVKNDVLAVLAPVAVETAAPVPK
                     AEEKPAKELPEGVEIIKMSPMRKAISKGMVNSYLTAPTFTLNYDIDMTNLMALRKQVL
                     EPIMNKTGLKVTFTDLIGLAVVRTLMKEEHRYMNASLINDAQEIELHKFVNLGIAVGL
                     DDGLVVPVVHGADKMSLSDFVVASKDVIKKAQSGKLKGAEMSGSTFSITNLGMFGTKT
                     FNPIINQPNSAILGVASTVQTPVVIDGEIKIRPIMALCLTIDHRIIDGMNGAKFMVDL
                     KNLLENPLELLI"
     gene            22708..24468
                     /gene="lpdA"
                     /locus_tag="AC1PIW_000433"
     CDS             22708..24468
                     /gene="lpdA"
                     /locus_tag="AC1PIW_000433"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIP6605397.1"
                     /translation="MAIEIIMPKLGVDMQEGEIIEWKKQEGDFVNEGDVILEMMSDKT
                     SMELEAEESGVLLKIVHGNGATVPVTEVIAYLGAEGESVEVGAALAPAEVAQATADLK
                     AAGLEVPATPAAAPQAPKAELAADEYDMVVVGGGPAGYYAAIRGAQLGGKIAIVEKSE
                     FGGTCLNKGCIPTKTYLKNAEILDGLKIAAERGINLASTNYTVDMDKTVDFKNKVVKT
                     LTGGVQGLLKANKVTIFNGLGQVNPDKTVVIGDKVIKGRSIILATGSKVSRINIPGID
                     SKLVLTSDDILDLREIPKSLTVMGGGVVGVELGLVYASYGTEVTVVEMADRIIPGMDR
                     EVSVELQKVLSKKGMKFLTSVGVSEIVEANNQLTIKLNDGSEIVSEKALLSIGRVPQL
                     AGLENLNLELDRGRIKVNEYQETSIPGIYAPGDVNGTKMLAHAAYRMGEVAAENAIHG
                     NHHKAKLDFTPAAVYTHPEIAMVGLTEDQAIEKYGKENILIGRNSFTGNGRAIASNEA
                     HGFVKVIADKKYHEILGVHIIGPVAAEMINEAATIMESELTVDDVAASIHGHPTFSEV
                     MYEAFLDVLGVAIHNPPKRK"
     gene            complement(24520..25710)
                     /locus_tag="AC1PIW_000434"
     CDS             complement(24520..25710)
                     /locus_tag="AC1PIW_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6688 domain-containing protein"
                     /protein_id="HIP6605398.1"
                     /translation="MSKHLKSFLIFYLAASIFLPFMWFINAPLICFVLPLYLTWKNIR
                     HFWDLLKKQLKEYSFWINDGLIFFLGTGLSWLALEMAQVVYVDWPETLVNNQIHSPMQ
                     TEAWSGQFFLLLLGVLAYLVLNIFQTKLLPPLLTVLLISCLYPSFVFAVLWTIQLSSL
                     IETDFFTYCYLCLVPFNICLIYSRTILQTIQLWQAELAKQSNPRFPRLSALLQKSLSL
                     PIWLLFFSLPYLAVLGSYLILFGQKPDQLLQMWTETSDWALSEKISPPNAFYDEHYLC
                     TVGAAGHRKLVKPIRMGERHGHRVVVNRQLQIANAFEQILEERCPRLHRCVRSNYDRY
                     GYPISKHIRKAWQADLIYLIMKPAEWLFLIVIYLHDRQPENRIAVQYLPLSKNLLPQE
                     NTSN"
     gene            25840..26829
                     /locus_tag="AC1PIW_000435"
     CDS             25840..26829
                     /locus_tag="AC1PIW_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014677692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HIP6605399.1"
                     /translation="MKYIVNNSNDPAYNIALEAYAFKELTDIDEIFILWINEPAIIIG
                     KHQNAIEEINKEFTDEKGIHVVRRLSGGGAVYHDLNNLNYTIISNKADEGAFDFKTFS
                     KPVIDTLATLGVEANFTGRNDLEIDGKKICGNAQAYAKGRMMHHGCLLFDVDMSVLAS
                     ALKVSKDKIESKGVKSVRARVTNINNELPDKMTVLEFKDAILNQMKQEYPEMDEYVLT
                     EDDLARIQEIRDSQFATWDWTYGQTPEYTVERSVRYPAGKITTYIKAEKSVIESIKIY
                     GDFFGIGDVADVEDLLVGTRYEYADVLAKLQEIDTTHYFSRMTTEEVAKAIVA"
BASE COUNT         8148 a         4945 c         6305 g         7478 t
ORIGIN      
        1 tgttttccgc acacgcaagc tatccagttg cttcttggct tctaagggat tgagtcgtgt
       61 ataagcgtag ccatgttcat ccagaaaagc ttgaagacga cgagaataga cgcctgttgc
      121 ttcaaagatg atttctggct tgtggacggt tttcaaatcg ccaagtagcc gagaaaagcc
      181 aatggcatca ttggacatgg tgtagttatg taccttctcg ccgttgacta gaatggccac
      241 ttctgaactt gccttactca catcaatccc aaaaactact cgcatgatat tacctctttg
      301 tcttgaatga ttccttgttt tagtgatgtc attttcaata ctcgacgtct ggcgtcccac
      361 atactttgat aacattcttt ctaaaacagg tgtcttgcca gtttttgttg cgacgtctag
      421 cgtcaaaaga gttctcgact taacaagaca cctctacttt atcataaaga aaaagtagtg
      481 agtactttct cccgtcggag atttcctcac tactaatctt agtatgtttt tgtaaaatcc
      541 gatgtaacaa gcttgttttt tacagaaagc agaaaatgtg gtaaaatgga gtgataaaac
      601 tatttgaggt aacgaattat gactgttaaa atcaatacta aagacggcca aatcgaattg
      661 actgatgacg tgattgccac agttgttggc ggtgctacga ctgagatttt cggtgttgtt
      721 gggatggcta gtaagtctgc gatcaaggat aattttcaag cacttttgag aaaagaaaat
      781 tattctaaag gtgttgtgat taagaccttg gaagatggtc gcattgcagt cgatgtttat
      841 actgtgatga gctacggtgt aaaaatcagt gaagtatctc gcaacatcca agaacgcgtt
      901 aagttcaact tggaaaatca actaggtatc tctgtagatg ccgtcaatgt ctttatccaa
      961 aatatcaaag tcgtaggaga ataattgtgt ctaaaattac aactagttta tttcaagaaa
     1021 tggtgcaggc agcatcaact cgtctgaata aacaagccga atatgtgaac tcactcaatg
     1081 ttttcccagt tccggatggg gatactggta ccaacatggg gatgactatt accaatggtg
     1141 ctaaagaagt agcagataag tctgcttcta ctgttggaga agttgcgcaa attctttcta
     1201 agggcttgtt gatgggagct cgtggaaact caggtgttat tacgtcgcaa ttgttccgcg
     1261 gttttggtca atctgttaaa ggcaaggttg aattggacgg taaggatttg gcgcaagcct
     1321 ttcaacatgg tgtagaagtg gcttataaag ccgttatgaa gccagttgaa ggaacaattc
     1381 ttactgtttc gcgtggggca gccactgccg ctttgaaaaa ggcagaagag actgatgatg
     1441 cggttgaggt catgcgtgcc actcttgacg gtgctaatcg tgctcttaag aaaacgccag
     1501 agatgcttcc agtcttgaag gaagttggtg ttgtcgattc aggtggacaa ggtcttgttt
     1561 acatttatga aggcttcttg tcagctttga cgggtgagta tattgcttcg gaagattttg
     1621 aggcgacacc tgctgttatg tctgaaatga tcaatgctga gcaccacaag tcagttgcag
     1681 gtcatgtagc gactgaagat attacctttg gttactgtac agaaatcatg gtagctcttc
     1741 gccaggggcc tacttatgtc aaggactttg attatgaaga attccgtaac taccttaacg
     1801 accttgggga ttccctcctt gtggtcaacg atgatgaaat cgttaaagtc catgtccata
     1861 cagaagatcc aggtttggtc atgcaagcgg gtcttcaata tggtagcttg gtcaaggtaa
     1921 aagtggacaa tatgcgtgag caacatgaag ctcaggttga gaaagtagaa agtttccaaa
     1981 aaccggctga acaaaaagag tatgctatta ttgcggtagc tgctggggaa ggtttgacag
     2041 aaatcttcaa atcacaaggc gttgactatg tgatttcagg tggtcagacc atgaacccat
     2101 caacagaaga ttttgtcaat gctattgaat tggtcaatgc ccgcaatgtc atcttgttgc
     2161 caaacaacaa aaatatcttg atggctgctc agacggctgc tgaggttgca gaagttgcgg
     2221 ttaaggttgt ggaaactaag accctaccac aaggttttac tagcttgctt gcttttgatc
     2281 caagtcgtga tttggaaagc aactttgaag cgatgacggc atctctcgca gaggtgaaaa
     2341 gtggtagcgt gacaactgcg gtccgcgata caaccattga cggtcttgaa atccatgaga
     2401 atgacaacct tggtatggtt gatggcaaga ttgtggtttc taatccagat atgatggaga
     2461 cacttgtggc tacctttgat aaaatgttgg atgaagacag cgaaatcgtg actatctaca
     2521 tcggtgaaga tggtaatgaa gaattggctc aaactcttgc tgaacagcta gaagaacaat
     2581 ttgaagatgt tgaggtcgaa atccatcaag gaaatcaacc agtctatcca tatcttttca
     2641 gcgtagaata aacaaaatcg caggctctgg cttgcgattt tttctgtcga cttgtctaca
     2701 atttccagta gacctatgac aaactactgg ggctatggta taattaaaga catgtatagt
     2761 cagaatgaaa atgaattgat tgccattggt gagagaattg gaaaggcctg taagccaaat
     2821 caagttctag tattatcagg ggatttgggt gctgggaaaa caactctgac caagggtttg
     2881 gccaaggggt taaaaattga acagatgatt aagagtccta cttatacgat tgttcgagag
     2941 tatgaggggg ccatgccgct ctatcactta gatgtttatc gaattggaga tgaccctgac
     3001 tcgattgatt tggatgattt tctctatgga ggaggtctaa cggttatcga gtggggagaa
     3061 ttactggatg tcagtctatt tgatgactat ttgctcattc gtatagagaa agagggagat
     3121 ggtcgacgat tgacagtcga atctcatgga gctcaaagta gcgatttagc gaaggagttc
     3181 caaaatgtct gaaaaagaag tgtattttag tgaagctgaa ccagctgatg cagcagcttt
     3241 tattgatttt atgaatcaag ttgctagaga aacggattat ctagtcatgg atgagactgg
     3301 ttttcgattt agtgttgatg aaatagagac catcttcgac gcagggatcg aaaatcctcg
     3361 tgaattgtgc ctgttggcta aggttggttc ggaagtcgtc ggcgcaattt ctgttaagtc
     3421 ctctaagcag tttcgcatta gccatatcgg aaatattttt attgcagtaa aaaaagccta
     3481 ctggggtcac ggtataggga cgattttact ggaggaagta ttgcattggg cagaggaaat
     3541 ggatgtattg aagcgtttgg aattaacggt tcaggttcgc aatcaggcgg cagtacattt
     3601 ataccaaaaa ctaggattca acattgaagg aacccagata ggtggggcta gaacagatga
     3661 aggagaatgg cttgatcttt attatatggg caagttaatt ggtgaagtat gacaattgga
     3721 aaaaaaatat tcttaatgtc tttggctatt attggtttaa cagtaggtgc cggcttaata
     3781 tatggagcca gcttgttaaa tttttctacg gatgctattt cgaaaacatt taagcagcta
     3841 gatggagaag aaaaaataac tccgattgat gcgacagagc ccctaactat cctactgatg
     3901 ggggtggata tggaccaagc gacccgcggg ggagattggg agggtggccg tagtgattcg
     3961 atgattttgg taactgttaa tcccaagact aaggaaacca atatgatgag cttgactcgt
     4021 gatattatgg ttgagattgc agaagcgaat ggggagtctt caggaacggt tgaaaaacta
     4081 aatcattctt atagctacgg acaggcacca atggccattg ccacgatcga aaagatgatg
     4141 gatattaaca ttgaccgtta tatcgaaata aatatggatg gtctggttga gcttgttgat
     4201 gcggttggag gtattgaggt aaacaatact cttggcttcc ctatatccat ttcggaacat
     4261 gagcctgcct acacctctat tgtccagccg gggaaacagt tggtaaatgg ggatcaggcg
     4321 ctggtttatg ctcggatgcg ctatgatgat cctgaggggg atattggtcg tcagagacgt
     4381 caacgtgaag ttataacggc tattataaaa aaactccttc agttagacgg ttttacccag
     4441 tataagaaga ttctgactgc catttctaat aatatgcgaa ccaacattga gattagtcct
     4501 gtaacaattc caaatctctt ggggtacaag gactctgtct ccaagttgaa ttcctatcag
     4561 ttgcgcggtg tggatcaaat ggttgatgca atctattatc aattaccaac cagtacacat
     4621 ctattggaaa tgcaaaatgt tctgaaaaaa tctatcggct tagaagaaaa gaccgactta
     4681 gtcacaaacg tgaaggtcta cgaaggacag atgggattgc caagtccaat aaacgtttat
     4741 gatgcatata caaatctctt gctgttagaa gcaagtccat gggcagaaag cttggatcca
     4801 gaaataggga gtccatcggc tactacgact gtgacaacag aagagcaggt aaccgatttt
     4861 tcaacagagc cagtactcgg tgatgaaaac ggggcacaat aatgtagtca ttagtttttc
     4921 ttttattact ttgacgagcg agtactaaaa gcctataaaa atagaggttg ttttcaacct
     4981 ctatttttat atatttggaa attccagatg ttagggagtg ttattctcat tctggtattt
     5041 tttgagaatc tgttggattt cttccaagtt tcctgcaata ctttgctgat agactcgagt
     5101 cttatattgt aagtcagaag ccatttcctg caagggctct tgaaaacttt ggataatggc
     5161 aagttgttcc tgaatattgt ccttgctcag ttgaatatct gatagaagtt gtttggtttc
     5221 ctttatttct ttggtaattt tatcccgatg cgtgactgca aggaagctgg cggtagcggc
     5281 gcttacggct aaaatgatat tacgaagttt catattattc tcctagatag agctcttgtg
     5341 ccagttgagc cagggctggg aagtcagggg cgttggcttt aaattgaatg ctcaatccat
     5401 cttgattgtt aaagcgtggt aagagatgga tgtgtgtatg aaagacggtt tgcccagcgg
     5461 cctcctcgtt attattaacg atgtttagac cgctagctct aagtttttct tttaactgac
     5521 gagcgatggt tggcacaaca gaaaagagtg tggctgcagc ttcttcatcc atgtcaagca
     5581 cgtttcggta gtgtttttta gggacaacca atgtgtgtcc ttttgtgacc tgagtgatgt
     5641 ctaaaaaggc taatacttgg tcatcttcgt agacttttga agctggtatt tctccagaaa
     5701 taattttgca aaaaatacaa tccgacataa tggcaacctc tttctaggat ttttgttata
     5761 ataattatat caaattatag gaagaaatgt atgttagaag taaaaaatgt aactggagga
     5821 tatgtcaata tcccagtttt gaaagatgta tcttttaccg ttgaggatgg tcaattagtt
     5881 ggtctaattg gtttaaatgg tgcagggaaa tcaacgacta ttaaggaaat tatcggtctt
     5941 ttgacaccct atcaaggaca gattgtaatt gacggcctaa ctttgctaca aggggctgaa
     6001 aattatcgga agaaaattgg gtttatccct gaaacaccaa gtttgtatga agaactaacc
     6061 ctcaaagagc acttggaagt ggtagcaatg gcctatgatt tatcctggga gcaagcttgg
     6121 ggacgtgttg agcacttgtt aaaactgttt cgcttggatg agaaattgga ctggtttcct
     6181 gtcaattttt caaagggaat gaagcagaag gtcatgatta tttgtgcttt tatggtggag
     6241 ccgagtctgt tgattgttga tgagcctttt ctgggattag accctgtggc tatctctgat
     6301 ttaatcgctc ttttggggga agaaaaatct aagggcactt caatcctcat gtccactcac
     6361 gttcttgact cagctgagaa aatgtgcgac tcttttgtga ttttacatca gggacaggtc
     6421 cgtgcaactg gaaatttgga ggagctacaa agcgcatttg gtatggatgg tgcaagtctg
     6481 aatgaaattt atctggcctt gacgcaagaa ggagtggctg gatgaaagaa ttgtttcaac
     6541 aacgccgact agatttttta aatcgttgtt ccaagtatct gcgctatgtt ctgaatgacc
     6601 attttgtttt ggttctcatg gttttattag gctttttgag tttgcaatat cgtcaattat
     6661 tgttgtcatt ccctgagaat ccgttgccga ttgttttggt ggttattgca atcagtctac
     6721 tggtgctatt gtccggaaga attgcgactt atttagaaga agctgatcaa atcttcctat
     6781 tgaccaagga aaaagacatt ctgcaaggac tcgaggttgc agccagacgc acggtcttgt
     6841 tttggtcctc tatacaggtg gtggttcagc ttgttttgtt accaatctat ttgaaactgg
     6901 gtttaccggt gtggatgatt gtatctggtc ttcttacttt gttggtttta aaatgtcttt
     6961 ttgtcaagcg ccaattagtg gcttatcagt cacagggagt attggactgg tctaaggcta
     7021 ttcaagatga acaaaaaagg aaacagtcta ttttacgttt ctttgcctta tttacaacgg
     7081 ttaaaggaat cggtacgact gttaaaccga gaagctatct caatggaatc ttgcgtctcg
     7141 tcaagtcgag acagacttgg ttttatctct acttacgggc tttcttgcgt tcaggagact
     7201 acttgggctt gacgatacgt ctactgttgt tggcgatttt gtccctattt gccattgaag
     7261 aatcgtggtt atccatcggc ctagttttag tctttcatta tttgcttgtc ttccagttgt
     7321 tggggcttta caagcattat gattatcaat atataacaca actgtatcct ctggagaaag
     7381 attccaagaa aattggtctt cagaatttgc ttagagtgat tttatatagt ctattagtag
     7441 tggaattggg ctttgctctg ctattttcta aagagaatgc gatggtagtc gtcttacttc
     7501 tcgtggggat tttcttgcat gaaatatatt tgcctctaaa attaaaaaaa ttgattgact
     7561 aaatagacga aaattagtaa aatagtaagg aatttttaaa ggaggaaagg catgcagttt
     7621 gataccagtg ggctacaatt acagtctatt gcaggaaata gtggcaaagc gtttaaaggc
     7681 ctacggtcag acggaacgcc agtctttgtg aagtatgaga tgcctccgat agtgtctgct
     7741 cttgcgcgtg agcaaattac accacccgtt ttgtcagcaa atcgtgaagt aggaatgggg
     7801 catcgtgtag agcaggagtg gttgaatggt cgtaccttgg agcgtcaaga tatgactagt
     7861 aaacagattc gtcaaatact ggtgagaatg cactattctc gcattttgct caatcaggct
     7921 ttgcagatga attataccta tatggaacct caggatttgg tttatcgttg gcaaaaagag
     7981 gcaccaacta gattgggaca gaattcttat ctgcaatcta tctgtcaaga tttattgaat
     8041 aatctaccaa gattccgtca agaggtggca acatttgttc atggagattt gcatcatagt
     8101 aactgggtag aaacgacgtc tggtttggtg tatttgacag attgggagac cgcttgtgta
     8161 acggatcgga tgttggatgt tgcctatatt ttgacgcatt atattcctcg tcagtcctgg
     8221 gaagagtggc ttcgggctta tggctacaaa tataacagca cagtattaaa taaggtttat
     8281 tggtatggcc agcttggcta tctaaaccaa atagctaagc atgtagaaag ctacaatgtt
     8341 ttggctgcaa ataaagaaat ttatgctcta cggcaattcc gtcagagttt ctataaggaa
     8401 gtatgagagt tagaaatcgt aagggagcga gtgagcttct agcaaacaat ccccagtatg
     8461 ttatctccaa tccagaggag tgtaagggga aatgggcaga gatttttgga aacaataatc
     8521 ctatccacat agaagttggt tctggtaaag gtcgctttgt cactggtatg gctgcgcaaa
     8581 atccagatat taactatatt ggtattgata ttcagatgac cgtgttaagc tatgccttgg
     8641 accgtgtgct tgaagctggt ttaccaaata taaaattatt gcaggtggat ggttcgagtt
     8701 tgaccaacta ttttgcaccg gcagaaatag atcagcttta tctgaatttt tcagatccat
     8761 ggcctaagaa acgtcatgag aaacgccgtt tgacctacaa gtcattcttg gatacctaca
     8821 aggaaatcct acctgagaag ggagaaatcc acttcaaaac agataatcgt ggtctgtttg
     8881 agtatagcct ggctagtttt tctcaatatg gcatggtctt gaagcaggtt tggctagatt
     8941 tgcatgcgga tggtttggaa ggtaacgtta tgacagagta tgaggagaaa ttctcaaaca
     9001 agggtcaagt catctatcgt gtggaagcac agtttcaata attcccttag tggaaaatga
     9061 ggagaggtct aacagagacc tttttccttt gtcgaataaa ctatagtcat tcagtttttc
     9121 ttttattact tcgacgtaag aggaaattcc gtttccttat ttctaagttc aaacactcca
     9181 ctggattgtt tgaattcgct ttgccgtact cttcaggggt gacttgctcc gcaagggcta
     9241 tctccaacct tgcacagtca ctcgactgta caagctgtct gcagctattg aagtgaactt
     9301 gcttcgccct atctccaacc tccaaaggtt ccccaaacct ttggagagag tactaaaagt
     9361 aaactaaaag attataaatg tctataatcc atcgttttcc tttacaaata gggatagata
     9421 tgatagaata atggaagtga ataatgagaa aaggggttgg cgtcagtcaa ccctttccct
     9481 gtacacggat ttctgtatat agaatgaatg atagtacaag ggagtttact gtcaaaggaa
     9541 atagagagtc ctggatagtg cgaactattt gagtaatgta tttctagcga ctgtaaaaat
     9601 aggaggtgtt tatgtcatca attattgaat tggtcacagc agcaattacc cccgctattc
     9661 aaactcctta cgagttggta gatgtggaat atggtaaaat gggcggtgac tatgtcctat
     9721 cgatttttgt cgataaggag ggtggaattt cccttcaaga tacggcagat ttatccgaaa
     9781 agatcagtcc gattttggac actatcaaac cagacccatt tccagaccaa tacatgctgg
     9841 aggtaacaag tccaggtttg gaacgtcctt taaaaacggc agatgctgtt gagaaggcgg
     9901 ttggaaagta catccatgtc aaactctatc aggcaattga taaaatcaag gtttttgaag
     9961 gaacgttgct ctcctttgat ggaacagacc tgattatgga gtatatggat aaaactcgta
    10021 agaaggaagt aactattcct taccagacgg tggctaaggc ccgtttggct gttaagttat
    10081 aatgctcttt catgagtatt ttataaagat attagacttt aagaaaggac cttttgagcg
    10141 caaaaggaaa acaacatgag taaagaaatg ctagaagcct tccgtatttt agaggaagac
    10201 atgggtatta acaaagcgga tatcattgat gcagttacag agtcgcttcg ttcagcttat
    10261 aaacgccgtt atggtcaatc agaatcagca gtgattgaat ttgacgagaa aaaaggcgat
    10321 ttccatgtat ttactgttcg tgaagtagta gatgaagtat ttgatagccg actagaaatt
    10381 agtctaaaag atgccttggc tatttcatca gcttatgaaa tgggtgacaa aattaaattc
    10441 caagaagacc cagcggaatt tggacgtgta gctgctcaat cagctaaaca gaccatcatg
    10501 gaaaagatgc gcaaacaaaa gcgtgccatt accttcaata cctacaagca acatgaaaat
    10561 gagattatgt caggtacggt agagcgtttt gataatcgct ttatctatgt caaccttggt
    10621 acgattgaag cacaattgtc aaaacaagac caaattcctg gtgaggtttt ccaatctcat
    10681 gatcgcatcg aagtctatgt ctacaaggta gaagacaatg gtcgtggtgt taacgtattt
    10741 gtcagccgta gccatccaga aatgatcaaa cgtctcatgg aacaggaaat tccagaagta
    10801 tacgatggaa cagttgaaat catgagtgtt gcccgtgaag ctggagaccg tacgaaagtg
    10861 gctgttcgta gccataatcc aaacgtagat gctattggta ctatcgttgg tcgtggtggt
    10921 tcaaatatta agaaaatcac tagcaaattc cacccagctc gttatgatgc taagaatgac
    10981 cgtatgattc ctactgagga aaatattgac gttatcgagt gggtagcaga tgaagcggaa
    11041 tttatctaca atgcccttgc acctgctgaa gttgaccaag tattgtttga cacggaagat
    11101 gggaaacatg ctacagtcgt tgtaccagat gataagttgt cattggcaat tggtcgtcgt
    11161 ggtcaaaacg ttcgtttggc agcgcatttg acaggattcc gtatcgacat caagtcagca
    11221 tcggagtatg aagcttttga agcaactcaa tatgcaacag acgaagttgt agaagaagtc
    11281 gcagaagaac aagaataagg caaaaggagc aaccatggca aaagctagaa aaataccctt
    11341 gcgcaagtct gtggtgtccg gtgaaatcat cgacaaacgg gatttgctcc gtattgtaaa
    11401 aaataaagaa ggtcaagtat ttatcgatcc aacaggcaag gcaaatggtc gtggagctta
    11461 catcaagctt gacaatgatg aggcaagcca agctaagaaa cgtcgtgtct tcgatcgttc
    11521 ctttagtatg gaagtggccg aagaatttta cgatgaatta attgcctatg ttgatcataa
    11581 gatcaagagg agagaacttg gactcgaata agagacagca aatcttaaat ttattaggtt
    11641 tggcccagcg agctggtcgc ctggtttcgg gtgaggacct ggttgttgaa gctattcaaa
    11701 aagggcaggc aaagttggtg tttttagccg aagatgctgc tggaaattta agtaaaaaag
    11761 taacagataa aagtcatacc tatcaagtag aagttgtaac agtgttttca acactggaat
    11821 tgagtgcagc tgtcggcaag gctagaaaag ttcttgcagt gacagacgct ggatttacaa
    11881 agaagatgag gtctattatg gaatagaaca ggaggacaag gcattgtcta agaagagatt
    11941 gaatgaaata gcccgtgaat taggcgtaag cagtaaggaa gttgttgcta aggctcaaga
    12001 attgggtttt gaagtcaaaa gtcatgcttc aagcgtagat gaagcaagtg ctaaacgcct
    12061 tgcagaaagc tttgggggac aaaaatcaga ggcaactaaa gttgcggcaa aagttagcaa
    12121 gcctgagaag gttgatgaaa ctccaaaagt ggaaacagct aaggtagaaa aagctaagga
    12181 aacacagcca gtagtaaaag aagaagttgc ggcaagtgca gtacaatcag cttcacatcg
    12241 tccacagagt cgaaacttta aggcagagcg tgaagcacgg gccaaagagc aggctgctaa
    12301 gcgagctcag aatcaaggaa aaggtggtca agctaaatct gatcaggacc gtcgtgacaa
    12361 ccgtcaacta ggtcagggac gttcaaacaa tgagcgaaat gatcgccgcg acaatcgtcg
    12421 tgaccagcgt ccagaagagc ggaaagacaa ccgatttgga gaccgtcgcg ataatcgaga
    12481 caaccgtcgt caggacaatc gttcaggtcg gttagctcgc tttgaacaaa gagaggctgc
    12541 aaaaccagca ggacctaaaa ttgacttcaa agcgcgtgct gctgctttga aagcagaaca
    12601 aaatgcggag tatgctcgta ccagtgaaga acgttttcgt caggctcagg aagctaaaaa
    12661 gcaacctaag aagccaaaag aaattaagtt tgaagaaccg gttgtggaaa gcaaaccatt
    12721 tgtgaaacct gcacttgttg catcggtacc agagcaagtt gcagaaacta ctgtagatac
    12781 ccgtcgtaag aaacaagctc gaccagataa aaaacgtgac tttaatagtg acgaagaaga
    12841 tggtccacgg aaacaacaaa gaaatcgaaa tagtcaaaat caagtgagaa atcaaagaac
    12901 aagtaactgg aataacaaca agaaaaataa aaaaggcaag gctaaccaac ctgccaaacc
    12961 tgtcacagaa cgcaagttcc acgaattgcc aactgaattt gaatatactg ctggcatgac
    13021 cgtcgcagaa attgcaaaac gaatcaaacg tgaacctgct gaaattgtga aaaaactctt
    13081 ccttatggga gttatggcga ctcaaaacca atctttggat ggagatacta tcgaactcct
    13141 catggttgat tatggtattg aagcgaaaga aaaggtagag gtcgataacg cagatattga
    13201 gcgtttcttc gtagaagagg gctacctcaa tgaagaagag atgacagaac gcccacctgt
    13261 tgtgactatc atgggacacg ttgaccatgg taaaacaacc ttgttggaca ccctacgtaa
    13321 ttctcgtgtt gctactggag aagctggagg tatcacacag catatcggtg cctaccaaat
    13381 tgaggaagct ggtaagaaaa ttaccttctt ggatactcca ggacacgcgg cctttacttc
    13441 tatgcgtgca cgtggtgcat ctgttaccga cttgaccatc ctagttgttg cagcagatga
    13501 tggtgtcatg ccgcaaacga ttgaggctat caaccactca aaagcagcta atgtgccgat
    13561 cattgtagct atcaacaaga ttgacaagcc aggtgcaaac ccagagcgtg tcatcggtga
    13621 attggctgag cacggtgtta tctcaaccgc ttggggtgga gaatctgaat ttgtagaaat
    13681 ctcagctaag tttaaccaaa acattgatga attgttggaa actgttcttt tggttgctga
    13741 aatccaagaa ctgaaggcag acccaactgt tcgtgcgatt ggtacagtta tcgaagctca
    13801 tttggataaa gggaaaggtg cggttgcaac cctcctcgtt caacaaggga cacttaatgt
    13861 tcaagaccca atcgttgtcg gaaatacctt cggacgtgtt cgtgccatga caaatgacct
    13921 tggtcgtcgt gttaaaactg ctggtccatc tacaccggta tctattactg gtttgaacga
    13981 aacgccaatg gctggcgacc atttcgcggt ttatgaagat gaaaaatcag ctcgtgcagc
    14041 aggtgaagaa cgtgctaaac gtgctcttct taaacagcgt caagcaacgc aacgagttag
    14101 tcttgaaaac ctctttgata cccttaaagc aggtgaggtt aagtcagtta atgtgattat
    14161 caaggccgac gtacaaggtt ctgttgaagc tcttgcatca tcgcttcaaa agattgaagt
    14221 agagggagta cgtgtcaata tcgtccactc tgcagttggt gctatcaatg aatcagatat
    14281 tactcttgcg gaagcttcaa atgctcttgt gattggtttc aacgttcgtc caacagctga
    14341 agcgcgtagc caagctgaag cagatgatgt tgaagtacgt cttcacagca tcatttacaa
    14401 ggttatcgaa gagatggaag atgccatgaa aggtatgctt gatccagaat atgaagaaaa
    14461 aatcattggt gaggcaatca ttcgtgaaac cttcaaggta tccaaagttg gaacaatcgg
    14521 tggtttcatg gtcgtacgtg gtaaggttac ccgtgattca agtgttcgag ttatccgtga
    14581 tggtgttgta gtatttgatg gtaaactagc tagtttgaaa cgctataaag atgacgtgaa
    14641 agaagttggt aatgcccaag agggtggttt gatgattgag aactacaacg acttgaaagt
    14701 tgatgatacc attgaagctt acatcatgga agaaatcaaa aaataatatt ttctcactgt
    14761 caaagtcata gacaagtgat tttgtcgagg aagtgacaat ttaatagatt ttagtaggtt
    14821 ttaaagcaat agtttttaat ctacttggat tattcaagct ggggaaactc agcttttcta
    14881 tcatctaaga aaggaaagaa tatggcgaat cattttcgta cagaccgtgt cgggatggaa
    14941 atcaagcgcg aagtcaatga gattttgcaa aagaaagttc gtgatccacg tgtgcaggga
    15001 gtgactatta cagatgttca gatggtcggc gatttgtcaa cggccaaggt ttattacaca
    15061 atcatgagta atttggcatc cgataatcaa aaagcacaaa cgggtcttga aaaagcaacg
    15121 ggaaccatta agagagaact tggtcgtaag ttaactcttt ataaaattcc ggatttggtc
    15181 tttgaaaaag accaatcaat tgagtacggc aataaaattg accaaatgtt gcgtgctctt
    15241 gatcagaaag attaacaaca aaagtcggat ggcaacattc ggctttttct ggctcggatg
    15301 ctttgtccat atatggtggc gtttacaagc cctcggaaag atgttatact aatactcaat
    15361 gaaaatcaaa agtagactag gaaacgaagt cgaagataga actctgttac tatcttattt
    15421 catggtaaca ggctgaaaac ctccactgga gcttttcact catcaaggca agttgacaac
    15481 ggataatttt gattttcgaa gagtataaga ccaaagatag agcacaggag gaagttatga
    15541 aaatatttat tagtaaactg atgttagtgt ccacggttgt actattagct gcttgtcagt
    15601 ctaatcaggt aaaaggtggt gagagttcta gttctagtgc ttcaagccaa gtttcagatg
    15661 gttctagttt gcagcaagcg tcaacaatag catctagctc cgagacgcaa gcatcctcag
    15721 tgaccactcc tgagctaagt gaagtatacc aatcagttat tgatcgttat caagcgaata
    15781 tgggtcagcc tgctgaggca attaatcagg atgaggccag tagctatctg agcttattaa
    15841 ctagccaggg gcaagaatac agtggaatat tttatagttt gtatgatgtt aaccatgatg
    15901 gtacagagga gctgcttcta gctttggaca agtcgggaga atatgtctta attgaccttt
    15961 atactcagct tgccgatgag agtctacgtt tggtggataa tttccgcaat cttggatttg
    16021 aaattggacc ggacgcccta cttcgtcctt tacaggatgg aacatactta tttgaaggag
    16081 ctggtgtttt tcgcatttat caatacaatg cgatgattcc aggtttaaaa cggatttctg
    16141 aatcagatac caatcctgaa acttctccct tgcttgagtt gaaaagcttg cattgggtta
    16201 agctatagat tttatcaatg attattccta ggctgggttt cccggccttt tgtagttcct
    16261 aagaagttta agttttcttt tggcaatctt taatctttct tttaggaagg aaaggtaaac
    16321 taggagcata tcaggaaggg atacgacttc ctaaagaaaa ggaatctaca ttatgaaaac
    16381 aaatcttaaa aaattacttt actctggtgt tactcttatg agccttggtg tcttagcagc
    16441 atgttcatct acttcaagtt caacgactac ttcaagctca gcagcaacta gtcagacaag
    16501 tgtagcgaca acttcaaata atagcacttc atcagattca agttcgtcaa ctattgactg
    16561 gtcagccctt ccaacgacag aagtgaagct ttcaaatgat ggcttgaaaa ttactgaggg
    16621 tggaacctat attttaactg gttcaacaac agctggtgta acggttgaaa cagatgccaa
    16681 tgttcgcatc attctagcag gtgctgagat ttcaagttca gatacagcag caatcaatgt
    16741 tattaatgct gacaatgttg aattggaaat caaagatggt acgacgaata cagtgaaaga
    16801 taccagcaat catacagaca ccaatattga aggggctatc catgttgaag ctgatttgac
    16861 catcactggt aatggtagcc tgaccgttga aggcaacttc caagatggca tcgtatcgac
    16921 agatgacctt gtcgtgaatt caggaaatat taccgtcact gcagtggatg atggtatccg
    16981 tggtaaggat tcattgacaa tcaatggtgg tacaatcaat gtaacagcag gtggtgatgg
    17041 tatcaagtct acaaatgata cagatactac caaaggttat acaactatca caggtggtga
    17101 aattactgta acagcaggtg atgatggtat taaagcggag acggctttga caattgacgg
    17161 aggtaccatt acagtatcag aatcagcaga agccttggaa ggaactaata tcactatcaa
    17221 tggtggtaca ttagatgtct atggttcaga tgatgctatt aatgcggcaa gtacaacatc
    17281 atcagaaatc ttcatcaagg taactggcgg tgacttgaaa gtagctgtag gtagtggaga
    17341 tacggatgcc tttgatgcta atggagacat ttatatctca ggtggtacaa ttgatgtaac
    17401 agctcaatct gcttttgact tcgacggaac tgctgaattg acaggaggta ctgtgacagt
    17461 taacggtgag cagattactc aaatcacagc aacaggacca ggtgctggtg gccacggtgg
    17521 ctggtaatca ataataatac aaaaaggtct gagttgacga actcagacct ttcgtttgct
    17581 ttccatattg ggaagattga ttttgtattt aattaccaat aatcgaatga ttaaagaaat
    17641 ggtaaaaata gtgatgaagg ttggaatttg tggtaatttc caatttacaa cgcaaagatg
    17701 atagaggact ccagagaaaa atgcaaggac agcatagaca tcttctttaa gaacaatggg
    17761 tgtttcattg acgagtaaat cacgggtgat tccgcctccg acacctgtca aagctgccag
    17821 aataccactg gttagagcgt ttagttggag agagatgcca gtactggctc caatgagggc
    17881 gaagatggct agaccgatcg catcaaagat aaggtttgtc tgggaaagga aatgaatcat
    17941 cttcttatca agtggtttgt cctgctttag attgataaca atcaagtaca tgctaatcgc
    18001 tactccgaga gataaataga tggctgtagg atcagtcaag gcggcaggaa tgcgattggc
    18061 catcgtatct cgaataattc ccccaccaac ggcggtaatg ataccgagca agctaattcc
    18121 aaaaatatct aactttttct taaatccttt tacggccccc gaaacagcaa atgcgatagt
    18181 gcctatgtag ttacaaatca taagaaataa atcgaattcc ataagaacct ccgtacctat
    18241 attaacagaa agaaagacgg cgtgcaagct tatttcaaag tttgtgaaaa aagtttcaaa
    18301 caagtaaagt gaatctttta atgatttgtg aaaaaacgca ctaaatcaag gttttcacaa
    18361 aaatttctgt aaaaagaaaa cggttcaatt acttgccatt tgtgaaatat tggtctatca
    18421 tagtacagaa agagaaaaac ctttggagga aattatggta tctatcacaa aagaacaaca
    18481 tttggacatg ttcttgaaaa tgcaacaaat ccgtgatgtt gacatgaaat taaacaaatt
    18541 agtgcgtcgc ggtttcgtac aaggtatgac tcacttctca gtcggtgaag aagctgcggc
    18601 tgttggacct attgctggat tgacagatga ggacatcatc ttctcacacc accgtggtca
    18661 cggtcacgtt attgcaaaag gtattgacat caacggcatg atggctgagc ttgctggtaa
    18721 ggcaacaggt acatctaaag gtcgtggtgg ttcgatgcac ttggctaacg ttgaaaaagg
    18781 aaactttggt tcaaacggta tcgtaggtgg tggttatgcc cttgcggtgg gtgcggccct
    18841 tacgcaacaa tacctcggta cagataacat tgttattgcc ttctcaggtg attcggctac
    18901 taacgaaggt tccttccacg aatcaatgaa cttggcagct gtttggaact tgccagttat
    18961 cttcttcatt acaaacaacc gttacggtat ttcaactgac atttcttatt ctactaagat
    19021 tcctcacctc taccaacgtg ctgctgcata cggtattcca ggtcattatg ttgaggatgg
    19081 taacgacgtt attgctgttt atgaaaaaat gcaagaagtc atcgagtacg ttcgtgcagg
    19141 taaaggtcca gccatggtcg aagttgaatc ataccgctgg tttggtcact caactgccga
    19201 tgcaggtgtt taccgtacta aagaagaagt caatgagtgg aaagcaaaag acccacttaa
    19261 gaaataccgc aaatacttga cagaaaacaa gattgcgaca gacgaagaat tggatgccat
    19321 cgaagcacaa gtcgcagaac aagtagaagc atctgtgaaa ttcgcacaag aaagcccaga
    19381 tccagacatc tcagtagctt atgaggatgt gtttgtagac tagggtgtga ggacgttaaa
    19441 gaggcacaga aaaattagag agtcagctag tgatgtggac atcactaatg gctcatctat
    19501 ttttccaagc ctctagtcag aacctaacta caaacgtaag aagtttgact aagctctaaa
    19561 tagcaagtca aactagggtg gactacctac tcaagttcga gtacaattct aaggtgtctt
    19621 aagctgggac aataaacaat tacatcgtag taattgtttg gttttcctta gttcagtata
    19681 gataaattga tataaattag gagtaaaaaa tggctgaaac aaaagtaatg gccttgcgtg
    19741 aagcgattaa cttggctcaa agcgaagaaa tgcgtaagga tgaaaaagta ttcttgatgg
    19801 gtgaagacgt cggtatctac ggcggtgact ttggtacatc tgttggtatg ttggacgagt
    19861 ttggtccaaa acgcgttcgc gacacgccta tctctgaggc tgcaatcgct ggttctgcgg
    19921 ttggtgcagc tcaaacaggt cttcgtccaa tcgttgactt gactttcatg gacttcatca
    19981 caattgccct tgatgcaatt gttaaccagg ctgccaaaac caactacatg tttggtggtg
    20041 gtttgaaaac acctgtaact ttccgtgtgg cttcaggttc aggtatcggt tcagcagcac
    20101 agcactcaca gtctcttgaa gcttggttga ctcacattcc aggtatcaag gtggttgcac
    20161 ctggtactgc taacgatgca aaaggtcttc tgaaatcatc tatccttgac aacaacccag
    20221 ttatcttctt ggagccaaaa gctctttacg gtaaaaaaga agaagtgaac ttggatccag
    20281 atttctacat tccacttggt aagggtgaaa tcaaacgtga aggtactgat gtaaccatta
    20341 tttcatacgg tcgtatgttg gaacgtgcct tgaaagcggc tgaagaagtg gctgctgaag
    20401 gtatcagcgt agaagtggtt gacccacgta cccttatccc attggataaa gaattgatca
    20461 tcgaatctgt gaagaaaact ggtaaggtta tcttggttaa cgatgcttac aaaacaggtg
    20521 gtttcatcgg tgaaatcgca tcaatcatca cagaaagcga agcctttgac tacttggatg
    20581 caccaatcat ccgtatcgct tcagacgatg taccagttcc ttacgcaaac attcttgaaa
    20641 acgcagtatt gccaaacgta gagaaaatca aagcggcaat ctataagcaa gtaaacaagg
    20701 gttaatttta tcgtcatcca cttatctttt ctggctgtgt tgatttggct tgcctaacgt
    20761 aagttatgcc tgcaccaaat cgccttgtca gaaaagcaat cggaaagacg ataaccctaa
    20821 tatgctagaa ataaaatgtt ctagcatgac taagaatgct tggaaaagaa ttgagccgct
    20881 tcgattatcg aagtctggtt tggttcttga caaggtaaga aactgaaaga aaggaattga
    20941 acccgtccgg aaaactcgga aaaaagataa atcgttacga aaatcaggat tttctacgat
    21001 ttcctatttt tcattcgttt tctgttcgga cttggtatct taataatggc tattgaaatc
    21061 attatgccta agctcggtgt ggacatgcaa gaaggtgaaa tcatcgagtg gaaaaaacaa
    21121 gagggcgatt ttgtcaacga aggcgacgtc atcttggaaa tgatgtctga caagaccagc
    21181 atggagctgg aagcagaaga gtcaggcgtc ctattgaaaa ttgttcacgg aaacggtgct
    21241 accgttcctg taacagaagt tattgcctac atcggtgctg aaggtgaaac ggttgaagcc
    21301 ggcgcttcat ctgctcctgc tgttgaacca gcagcggcta ttgaagaagt acctgcgggt
    21361 cgtacaccag taatcgttgc tcctgcaact gctgccaaac cacaaggtag tggcaaggtg
    21421 cgtgcaacgc cagctgcgcg taaattggcg cgtgagcttg gaatcgactt gggtcttgtt
    21481 ccaggaacag gtgctaacgg tcgtgtccac aaggttgacg tcgaagactt caagggagca
    21541 gctccaaaag caactccgct tgcagcccgt attgcagcag accaaggtgt tgacttgtca
    21601 acccttacag gttcaggtgt caacggtaag attgtcaaga acgacgttct tgcagtactt
    21661 gctcctgtag ctgtagaaac tgctgctcca gtaccaaaag cagaagaaaa accagctaaa
    21721 gaattgccag aaggcgttga aatcatcaag atgagcccaa tgcgtaaggc gatttcaaaa
    21781 ggtatggtca actcttactt gactgctccg acatttacac ttaactacga tattgacatg
    21841 accaacctca tggcacttcg taagcaagtc cttgagccaa tcatgaacaa gactggtttg
    21901 aaagtaacct ttacagactt gatcggtctt gcggttgttc gtactttgat gaaagaagaa
    21961 caccgttaca tgaacgcatc tttgattaac gatgcacaag aaatcgaatt acacaagttc
    22021 gtcaaccttg gtatcgcagt gggtctagat gatggcttgg tggttccagt tgttcacggc
    22081 gcagataaga tgagcttgtc tgactttgtg gtggcttcga aagatgtcat caagaaggct
    22141 caatctggta agttgaaggg ggctgaaatg tcaggttcga ccttctctat caccaacttg
    22201 ggtatgtttg gtactaaaac ctttaaccca atcatcaacc aaccaaactc agcaatcctt
    22261 ggtgtagcat caactgttca aacaccagtt gttattgacg gcgaaatcaa gatccgtcca
    22321 atcatggcac tctgcttaac catcgaccac cgtatcattg atggtatgaa tggtgctaag
    22381 ttcatggttg acttgaagaa cttgctggaa aacccattgg aattgttgat ctgagataag
    22441 atgcaaaagg cgttaagaaa aacgtatgaa aataggaaat caacgatgtg ctttgaggca
    22501 cgaggcgatt tatctttttc aataggtttt tagcctgtgc tcaatttggt aaagagaaaa
    22561 aataaagaaa gggaataaga gcgagagagt tttagcaatt gaacccgagc ggaaagctcg
    22621 gaaaaaagat aaatcgttta gaaaatcagg attttctacg atttcctatt tttcagtcgc
    22681 tttctcttcg ctcttggtat cttaactatg gcaattgaaa ttattatgcc gaaacttggt
    22741 gtagatatgc aagaaggcga aatcatcgag tggaaaaaac aagagggcga ttttgtcaac
    22801 gaaggcgatg ttatcttgga gatgatgtca gataagacaa gtatggaatt ggaagcggaa
    22861 gaatcaggtg ttcttttgaa aatcgttcat ggcaacggtg caacagttcc tgtaacagaa
    22921 gtcattgcct accttggtgc agaaggcgaa tcagttgaag tgggtgctgc actagctcca
    22981 gctgaggttg ctcaagcaac tgctgacttg aaagcagctg gtttggaagt tcctgcaaca
    23041 cctgcagcag ctccacaagc tcctaaggct gaattggcag cagacgagta tgacatggtt
    23101 gttgtcggtg gtggtcctgc tggttactac gctgctatcc gcggtgctca attgggcggt
    23161 aaaatcgcta tcgttgagaa atcagaattt ggcggtacct gcttgaacaa gggttgtatc
    23221 ccaactaaga cttacctcaa aaacgctgaa atccttgacg gcttgaagat tgcggctgag
    23281 cgtggtatca accttgcttc aacaaactat accgttgata tggacaagac agttgacttc
    23341 aagaacaagg ttgtgaagac attgactggt ggcgttcaag gtctcttgaa agctaacaag
    23401 gtaacaatct tcaacggtct tggtcaagtg aatcctgaca agactgttgt catcggcgac
    23461 aaggtcatca aaggtcgtag catcatcctt gcaacaggtt ctaaagtatc acgcatcaat
    23521 atcccaggta ttgattctaa gttggtattg acttctgatg atatccttga cttgcgtgaa
    23581 attcctaagt cacttactgt tatgggtggt ggcgtagtcg gtgtagaact tggtttggtt
    23641 tacgcatcat acggcacaga agtaaccgtt gttgaaatgg ctgaccgtat cattccaggt
    23701 atggaccgcg aagtgtctgt tgaattgcaa aaagtccttt ctaagaaagg tatgaaattc
    23761 ttgacatctg ttggtgtatc tgaaatcgtc gaagccaaca accaattgac aatcaagttg
    23821 aatgacggct cagaaatcgt ttctgaaaaa gcccttcttt caatcggtcg tgtaccacaa
    23881 ttggctggtc ttgaaaatct taaccttgaa ctagaccgcg gtcgtatcaa ggttaacgaa
    23941 taccaagaaa catcaatccc aggtatctac gcaccaggtg atgtcaacgg tactaagatg
    24001 ttggctcacg ctgcttaccg tatgggtgaa gtggcagctg aaaatgccat ccatggtaac
    24061 caccacaagg ctaaattaga cttcacacca gcagcggttt acacacaccc agaaatcgct
    24121 atggttggtt tgacagaaga ccaagctatc gagaaatacg gtaaagaaaa catccttatc
    24181 ggtcgcaata gcttcactgg taacggtcgt gctattgctt ctaacgaagc ccatggtttc
    24241 gtaaaagtta tcgctgataa gaaataccat gaaatccttg gggttcacat catcggtcca
    24301 gttgcagctg aaatgatcaa cgaagcagca actatcatgg aatctgaatt gactgttgat
    24361 gatgtggcag catcaatcca cggtcaccca accttctcag aggttatgta tgaagccttt
    24421 cttgatgttc ttggtgttgc aatccacaac ccaccaaaac gcaaataata agctaagcaa
    24481 aaagaaagtc catgtggctt tcttttttgt gatatattac taattgctag tattttcttg
    24541 tggtaataga ttttttgata gcggcaaata ctgaacagca atgcgatttt ctggctgacg
    24601 atcgtgcaga tagatgacga tgaggaagag ccattcggct ggtttcatga tcagatagat
    24661 cagatccgcc tgccaagctt tgcgaatatg tttggaaatg ggatagccat accggtcgta
    24721 gttggaacga acgcaacgat gcaggcgtgg acatctttct tctaaaattt gttcaaaagc
    24781 attggcaatt tgtagctgac gattgaccac cacacgatgt ccatggcgtt cccccatacg
    24841 aatgggtttg accagtttac ggtgaccagc agcccccact gtacaaagat agtgttcatc
    24901 atagaatgcg tttggtgggg agattttttc cgacaaggcc caatcactgg tttccgtcca
    24961 catctgaagt agctggtcag gtttttgacc aaagagaatg agataggagc caagaactgc
    25021 aagataggga agactgaaga agaggagcca aattggcaga gaaaggcttt tttgcaggag
    25081 agcagaaagt ctaggaaagc gtgggttgct ctgcttggct aattctgctt gccacagttg
    25141 aatggtttgt aaaattgtcc gactgtaaat gagacaaata ttgaaaggaa ccaaacacag
    25201 gtagcagtag gtgaagaaat ctgtttcaat caaactagaa agttgtatag tccacaaaac
    25261 agcaaagaca aaacttggat ataggcagct gatgaggagg accgttagga gaggggggag
    25321 gagcttagtt tggaagatgt ttagcaccaa ataagctaaa acacccaaaa ggagtaggaa
    25381 aaactgccct gaccaggctt cggtctgcat tgggctgtgg atctggttat taaccaaggt
    25441 ttctggccaa tcgacgtaaa caacttgagc catttcaaga gccaaccaac ttagccctgt
    25501 tcctaaaaag aaaatcaaac catcgttaat ccaaaaacta tactctttta actgtttttt
    25561 caaaaggtcc caaaaatggc ggatgttttt ccaggtcaga taaagaggaa gtacaaagca
    25621 gataagtggc gcattgataa accacataaa aggaagaaag atgcttgcgg ctagataaaa
    25681 gatcaaaaat gattttaagt gtttgctcat gaaaacctcc ttttatagcc taccatcatt
    25741 tttgagaaaa ttcaagaatg gacaagaaga gtttcaatac ttgtcttttg ttatcgtttc
    25801 cactataata aaaattagtt aacaataaag gagttttgca tgaaatacat tgtaaataac
    25861 agcaatgacc cagcttataa cattgccctc gaagcctatg ccttcaagga attaacagat
    25921 attgacgaaa tttttattct ctggatcaat gagcctgcca tcattatcgg taagcaccag
    25981 aatgctattg aagaaatcaa caaggaattc acagacgaaa agggcatcca tgttgttcgc
    26041 cgtctgtcag gtggtggcgc agtttatcac gacctaaata acctcaacta tactattatt
    26101 tcaaataagg cagacgaggg agcctttgac ttcaaaacct tctccaaacc tgtgattgat
    26161 acccttgcaa cactgggtgt agaggccaat ttcaccggtc gtaatgacct ggaaatcgac
    26221 ggaaagaaaa tctgtggtaa cgcccaagcc tatgccaaag gtcgcatgat gcaccacggt
    26281 tgcttgctct ttgatgtgga tatgtcagtc ctcgcaagtg ccctcaaggt cagcaaagac
    26341 aaaatcgagt ccaaaggtgt taaatccgtt cgtgcccgtg tgaccaacat taacaacgag
    26401 ttgccagata agatgaccgt tctggagttc aaggatgcca tcctcaacca aatgaagcaa
    26461 gaatatccag aaatggatga atatgtgttg actgaggatg atttggctcg aatccaagaa
    26521 atccgtgatt cgcagtttgc aacatgggat tggacctacg gtcaaacacc agaatacact
    26581 gtggagcgca gcgttcgtta cccggctggt aaaatcacaa cctatatcaa ggctgaaaaa
    26641 tctgtcatcg aatccatcaa gatttacggt gacttcttcg gtattgggga tgtagctgat
    26701 gttgaagact tactagttgg tacacgttat gaatatgcgg atgtacttgc taaacttcaa
    26761 gagattgata ctactcatta tttctcgcgt atgacaacag aagaagtcgc aaaagcgatt
    26821 gtagcttaat tcatagggct gccctttgtt tgggcagtct ttattttttg tgaaaa
//
LOCUS       DBNFCK010000013        26572 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.013, whole genome shotgun sequence.
ACCESSION   DBNFCK010000013 DBNFCK010000000
VERSION     DBNFCK010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 26572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26572
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.013"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            72..2597
                     /gene="secA"
                     /locus_tag="AC1PIW_000436"
     CDS             72..2597
                     /gene="secA"
                     /locus_tag="AC1PIW_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIP6605400.1"
                     /translation="MVTNVLRSLIENDKGELRKLEKMADKVFSYADEMEALTDEQLQA
                     KTAEFKERYNNGESLDDLLYEAYAVVREGARRVLGLYPYKVQVMGGIVLHNGDVPEMR
                     TGEGKTLTATMPVYLNALSGQGVHVVTVNEYLSTRDATEMGELYSWLGLSVGINLAAK
                     SPLEKREAYNCDITYSTNSEIGFDYLRDNMVVRAEDMVQRPLNYALVDEVDSILIDEA
                     RTPLIVSGAQGSETNQLYFLADNLVKSLTTEDYIIDIPSKTIGLSDSGIDKAEKFFKL
                     DNLYDIENVAITHFLDNALRANYIMTYDIDYLVNEDQEVMIIDPFTGRTMEGRRYSDG
                     LHQAIEAKEGVPVQNESKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVV
                     PIPTNRPIARVDHEDLLYPSLEYKFNAVIADVKRRYEKGQPVLVGTVAVETSDLISQK
                     LVAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGPGVRELGGLC
                     VIGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERIKVFMERMNLT
                     EEESVIKSKMLTRQVESAQKRVEGNNYDSRKQVLQYDDVMREQREIIYRQRQDVITAD
                     RDLAPEIKAMMKRTIERQVAGHFLGSKDEAIDGIIKFAHANLVEDDTLSKATFEAMNQ
                     KEIVEELYERALRVYDSQVKKLRDEERVREFQKVLILRVVDNKWTDHIDALDQLRNAV
                     SLRGYAQNNPIVEYQSEAFTMFNDMIGAIEFEVTRLMMKAQIHDNIERERTSQEAHTT
                     AVKNIMPNQSHAIQENVSFEGVDRNDPCPCQSGKKFKNCHGRK"
     gene            2712..3071
                     /gene="acpS"
                     /locus_tag="AC1PIW_000437"
     CDS             2712..3071
                     /gene="acpS"
                     /locus_tag="AC1PIW_000437"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HIP6605401.1"
                     /translation="MIVGHGIDLQEMEAIESARIKHQGFPKKILTEKEFERYQSLSGR
                     RQLEYLAGRWSAKEALTKALGTGIGKIGFHDIEILNTSKGVPYVTKSPFDGNIWLSIS
                     HSGNFVQASVILEEKDD"
     gene            3064..4167
                     /gene="alr"
                     /locus_tag="AC1PIW_000438"
     CDS             3064..4167
                     /gene="alr"
                     /locus_tag="AC1PIW_000438"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011226530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIP6605402.1"
                     /translation="MIESEHRPTQIRVNLDAIAENFEQVMTNLPPKTEAFAVVKANAY
                     GHGAVAVAKKLSSQAAGFCVSNLDEALELRKAGIEHPILILGVVPVRFLPVAHQLNIS
                     VTVASLDWLQDAKDLEADLSGLTVHLKLDTGMGRIGFRKIADLLQAIAILEELEYDIE
                     GVYTHFATADEVEQAHFESQLSVFKEFLDVLPITPRWIHASNSATSIWHADTVFNLVR
                     LGNILYGLNPSGRVLELPFGVQPALSLVSEIVHVKQVEAGTTIGYGATYHSTDSEWIA
                     TVPIGYADGLVRSLQGLSVLVDGQACEIVGRISMDQITIRLPRAYPLGQKVTLIGQDG
                     DKTISVQEWADRIGTINYEVVCLLTDRLPRIFE"
     gene            complement(5106..6461)
                     /locus_tag="AC1PIW_000439"
     CDS             complement(5106..6461)
                     /locus_tag="AC1PIW_000439"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIP6605403.1"
                     /translation="MKVIKFGGSSLASAGQLEKVFNIVQSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYKEYVNGKDVTASQEWIINRYQAMVDELGFTANSMKKIADSIISLAS
                     LPIDDNEFLYDAFLAAGEDNNAKLIAEYFTHRGLPARYVHPKKAGIIVSSEPGNARIL
                     PSSYDKIEELRDTDEVLIIPGFFGVTVDNQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIKNPHSIKELTYREMRELAYAGFSVLHDEALLPAYRGRIPLVIKN
                     TNNPTHPGTRIVHKHTEQTVPVVGIAADDGFVSINLSKYLMNREVGFGRKVLQILEDL
                     NIRWEHMPTGIDDLSIVVRERELTPIKEEEILRQLNTKMEVDKAEIEHGLSIIMIVGE
                     NMKSHVGVTATATTALSKQNVNLAMISQGASEVSVMFVVKTEEKKRALHALYEAFFNE
                     Q"
     gene            6638..7279
                     /locus_tag="AC1PIW_000440"
     CDS             6638..7279
                     /locus_tag="AC1PIW_000440"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIP6605404.1"
                     /translation="MVKAIIFDMDGVLFDTETFYFQRRADFLATKGLSVDHLDPSIFV
                     GGRASQIWQRILGDDYDNWDVPALEEEYRVYKEKRPTPYAERIFPEVKAVLERLTIQG
                     IPVVLASNTDRSEIERALLDAGIRFHFSHVFSAMDCEAPKPDPTVYMKAASATGVAKS
                     DILVFEDSAKGIEAAKKAGLTVWAIRDQHYGIDQSKADKLVDNLEDAFDLLRF"
     gene            7715..8506
                     /locus_tag="AC1PIW_000441"
     CDS             7715..8506
                     /locus_tag="AC1PIW_000441"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HIP6605405.1"
                     /translation="MAYQTIRYEVIDSVAILTLNRPEVANGFNIPMCEEILEAIRLAE
                     GDASVKILQIQAQGSIFSVGGDLVEMKRAVDDDDIASLVKIAELVNDISFAMKKLSKV
                     VIMVTDGAVAGAAANMAVAADFVIASSKTKFIQAFVGVGLAPDAGGLFLLSRAIGANR
                     ASHLAMTGEGLSADKALEYGIIYKLAEPEKLEKTVAQVIKKLLRGSVNSYAAIKQLTW
                     ESQFKDWESYRRLELDLQETLAYKEDFKEGVRAHAERRRPVFTGD"
     gene            8586..9020
                     /locus_tag="AC1PIW_000442"
     CDS             8586..9020
                     /locus_tag="AC1PIW_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIP6605406.1"
                     /translation="MEDPKVNEYLTAIFNNVLVIEETSLRGSRFNDISIKEMHTIDVI
                     GSIDGATPSDVARTLMVTLGTVTTSLNNLERKGYVERIRSTKDRRVVHLYLTKKGRLV
                     YRLHQRFHNEMVKQITDGMDDAEYQVMKKGLMKLYHFLEDLK"
     gene            9017..9994
                     /locus_tag="AC1PIW_000443"
     CDS             9017..9994
                     /locus_tag="AC1PIW_000443"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HIP6605407.1"
                     /translation="MRNHAKISQVAHYLPKKIVTNDDLAQRMETSDEWIRSRTGIGQR
                     HIVTGETTSDLASQVARKLLEKSQLDASEIDFIIVATITPDASMPSTAAMVQAAIGAK
                     NAFAYDLVAACSGFVFALSTAEKLLASGVYKRGLVIGAETLSRSVDWSDRSTAVLFGD
                     GAGGVLLEACEQPTFLAEILRTDGGRGASLTAGIDQKETPFSTQSCQQPFIQMEGRAI
                     FEFATRDVTATMAELLEQADMTVDCVDYFLLHQANIRILDKMARKLGVAREKFPANMD
                     KYGNTSAASLPILLSECVESGMLRLDGSQTILMAGFGGGLTWGTLLLQL"
     gene            10054..10278
                     /locus_tag="AC1PIW_000444"
     CDS             10054..10278
                     /locus_tag="AC1PIW_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIP6605408.1"
                     /translation="MAVFEKVQEIIVEELGKDAEEVTLTTTFEDLDADSLDLFQVISE
                     IEDAFDIQIDTEEGLTTVGDLVAYVEEKTK"
     gene            10451..11416
                     /gene="fabK"
                     /locus_tag="AC1PIW_000445"
     CDS             10451..11416
                     /gene="fabK"
                     /locus_tag="AC1PIW_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HIP6605409.1"
                     /translation="MKTKITELLDIKYPIFQGGMAWVADGDLAGAVSNAGGLGIIGGG
                     NAPKEVVKANIDKVKSITDKPFGVNIMLLSPFADDIVDLVIEEGVKVVTTGAGNPGKY
                     MERLHAAGITVIPVVPSVALAKRMEKLGVDAVIAEGMEAGGHIGKLTTMTLVRQVVEA
                     VSIPVIAAGGIADGAGAAAAFMLGAEAVQVGTRFVVATESNAHQAYKEKVLKAKDIDT
                     TVSASIVGHPVRAIKNKLSSAYAAAEKDFLAGKISADAIEELGAGALRNAVVDGDVTN
                     GSVMAGQIAGLVSKEESCEDILKDIYYGAAKVIREEASRWASVGE"
     gene            11418..12338
                     /gene="fabD"
                     /locus_tag="AC1PIW_000446"
     CDS             11418..12338
                     /gene="fabD"
                     /locus_tag="AC1PIW_000446"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIP6605410.1"
                     /translation="MTKTAFLFAGQGSQTVGMARDLYEYYPIVRETFDQASQILGYDI
                     RDLIDNQEDKLHQTRYTQPAILTTSVAIFRLLEEEGIQPDMVAGLSLGEYSALVASGA
                     LAFEDAIALIAKRGEYMELAAPAGTGKMVAVLNTDVNLIEEICATVTSGIVSPANYNT
                     PAQIVIGGEVAAVDEAVELLKEAGVKRMIPLNVSGPFHTALLRPASEQLAQALEQVEL
                     ADFQVELVGNTEAEVMKKEDIKSLLPRQVMEPVRFYESIATMQEAGVTNFIEIGPGKV
                     LSGFVKKIDKTAHVVAIEDIEGLQIVLNKS"
     gene            12348..13082
                     /gene="fabG"
                     /locus_tag="AC1PIW_000447"
     CDS             12348..13082
                     /gene="fabG"
                     /locus_tag="AC1PIW_000447"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011225529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HIP6605411.1"
                     /translation="MELTNKNVFVTGSSRGIGLAIAHKFASLGANVVLNGRGQLGQDL
                     LDSFADYGVKVLAISGDISSAQDAKRMVAEAIETLGSVDILVNNAGITKDGMALRMTE
                     EDFDTVLKVNLTGTFNMTQAVLKPMTKAREGAIINLSSVSGLIGNAGQANYSASKAGV
                     IGFTKAIAREVAGRNVRVNAIAPGFIQSDMTDVLSDKIKEAMTAQIPMKRFGATEEVA
                     DVAVFLAKQEYLTGQVIAVDGGLTMQ"
     gene            13108..14343
                     /gene="fabF"
                     /locus_tag="AC1PIW_000448"
     CDS             13108..14343
                     /gene="fabF"
                     /locus_tag="AC1PIW_000448"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIP6605412.1"
                     /translation="MTKLNRVVVTGYGLTSPIGNTPEEFWNSLKAGKIGIGKITKFDT
                     SEYSVHNAAELKDFPFDKYFVKKDTNRYDNYSLYALYAAKEAIANAQLDTETVDSDRF
                     GVILSTGIGGILEIEEQVARMNEKGPKRIRPMALPKALPNMAAGNIAMQVGANGVCKC
                     IITACASSNDALGEAFREIKFGFQDVMLAGGAEAAITPFAIGGFQALTAMSTTEDPER
                     ASIPFDKDRNGFVMGEGSAVLVLESLEHAEARGATILAEIVGYGNTCDAYHMTSPHPE
                     GLGAIKAMKLAISEAGLEPADIDYINAHGTSTPANEKGESQAIVSVFGKNTPVSSTKS
                     FTGHLLGAAGAVEAAAVIEAMRHSYAPKTAGTTELSEDIEADVIYGQGRDMEIRHAIS
                     NTFGFGGHNSVIAFKRWEA"
     gene            14344..14820
                     /gene="accB"
                     /locus_tag="AC1PIW_000449"
     CDS             14344..14820
                     /gene="accB"
                     /locus_tag="AC1PIW_000449"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIP6605413.1"
                     /translation="MNITEIKDLMSQFDQSSLREFSYSNAGEILHFSKNQQATVAPSP
                     AVEAPLAPVTPASPAPVPAVEMSEPAPAEAGASPVAEGAVVESPLVGVAYLSPAPDKP
                     AFVAVGDTVKKGQTLMIIEAMKVMNEVPADRDGVVTEILVANQDVVEFGQGLVRIK"
     gene            14817..15239
                     /gene="fabZ"
                     /locus_tag="AC1PIW_000450"
     CDS             14817..15239
                     /gene="fabZ"
                     /locus_tag="AC1PIW_000450"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HIP6605414.1"
                     /translation="MIDILKIKEALPHLYPMLLVDRVLEVSEDEIVALKNVTINEPFF
                     NGHFPDYPVMPGVLIMEALAQTAGVLELSKEENKGKLVFYAGMDKVKFKKQVVPGDQL
                     IMTAKFVKRRGTIAVVEAKAEVEGKLAASGTLTFAIGK"
     gene            15533..16906
                     /locus_tag="AC1PIW_000451"
     CDS             15533..16906
                     /locus_tag="AC1PIW_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIP6605415.1"
                     /translation="MFEKILIANRGEIAVRIIRSARELGIATVAVYSEADKEALHTML
                     ADEAVCIGPARSTDSYLNMQAVISAAVVTGAQAIHPGFGFLSENSKFATLCEEVGIKF
                     IGPSGTVMDTMGDKINARAEMVKAQVPVIPGSDGEVLTSQEALEIAEKIGYPVMLKAS
                     AGGGGKGIRKVEKAEDLVPAFESASSEAKAAFDNGAMYIERVVYPARHIEVQILADQH
                     GHVIHLGERDCSLQRNNQKVLEEAPSIAIGQTMRERIGQAAVRAAQSVGYENAGTIEF
                     LLDEAKGEFYFMEMNTRVQVEHPVTEFVTGVDIVKEQIKIAAGQELSVRQEDVKITGH
                     AIECRINAENPAFNFAPSPGKISTLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKVI
                     VHGENRFEALMKMQRALYELEIDGVVTNTDFQLDLISDKRVVAGDYDTAYLMEEFLPR
                     YQEDLKK"
     gene            16921..17787
                     /gene="accD"
                     /locus_tag="AC1PIW_000452"
     CDS             16921..17787
                     /gene="accD"
                     /locus_tag="AC1PIW_000452"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HIP6605416.1"
                     /translation="MALFRKKDKYIRINPNRSRIESAPQAKPEVPDELFSKCPACKVI
                     LYKNDLGLEKTCQHCSYNFRITAQERRALTVDEGSFEELFTGIETTNPLDFPNYLEKL
                     AATRQKTGLDEAVLTGKATIGGQPVALGIMDSHFIMASMGTVVGEKITRLFELAIEER
                     LPVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTVLTDPTTGGVTASFA
                     MEGDIILAEPQTLVGFAGRRVIESTVRENLPDDFQKAEFLQEHGFVDAIVKRQDLPAT
                     ISRLLRMHGGVR"
     gene            17784..18557
                     /locus_tag="AC1PIW_000453"
     CDS             17784..18557
                     /locus_tag="AC1PIW_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HIP6605417.1"
                     /translation="MTSDVARMIRLARDQARLTTLDYATQLFDDFIELHGDRNFRDDE
                     AVVGGIGFLAGQPVTVIGIQKGKNLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPV
                     VTFINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVAD
                     QVWMLENSMYAVLSPEGFASILWKDGSRAMEAAELMKITSYELEKLQVVDRVVLENGR
                     ATKEVLSDIKDNLVSQLAQLQALPLEQLLENRYQRFRKY"
     gene            18621..19094
                     /locus_tag="AC1PIW_000454"
     CDS             18621..19094
                     /locus_tag="AC1PIW_000454"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6605418.1"
                     /translation="MLVKADLSNAEELLPIQHRAFAALYKTYQDQYNPAIETMDYFQS
                     RFARPNCSYYKIIEEEHTVGLVRTTVAEDAEQGWLGLIGIDPDHRGKGYGHKAMLELE
                     SLYPTVRRWVLCTVLQESKLVSFYEKLGYKAIKTEPEQEGMDMVYMEKWIGDSDA"
     gene            19087..19719
                     /locus_tag="AC1PIW_000455"
     CDS             19087..19719
                     /locus_tag="AC1PIW_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605419.1"
                     /translation="MLEQCRRYLDYQGVHYCKPEKAGALAGDMIALKNVAQAARKEFQ
                     TLSLVLAEHVSPFVPERVSQWMNQAQICRPHFWTYYRFPSASLTDSALAIRLYGKGTE
                     AGISVEVSFIERKKVETSLLQQNRVLNCSIAAPLYYWVQEGQVFYRLDGTEENRQSLL
                     QAVEAGRIRKVLVKYDVPFRTVASEEELLERLIQGFSLLEPYLKMTHLAK"
     gene            19897..20142
                     /locus_tag="AC1PIW_000456"
     CDS             19897..20142
                     /locus_tag="AC1PIW_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605420.1"
                     /translation="MEKLGQSLKWLGRKIWLLLRIFGLLVLAVLPDLILEGSKSQVTL
                     VIVGLMTLGLLVFFWWRAEKMICQSGTLTFLLGTVWR"
     gene            20178..20555
                     /locus_tag="AC1PIW_000457"
     CDS             20178..20555
                     /locus_tag="AC1PIW_000457"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIP6605421.1"
                     /translation="MLGYYLLELQGVTTTENDASIIEFLKGVPFRLALLTTAFLPAVV
                     EEVIFRGYFFKKLFGSQVLLGIVVSSLVFGSFHGPTDLGSWLIDAGSGIILSLLYYKS
                     RYLIYPIIVHLVNNFIATVFYYI"
     gene            complement(20609..21535)
                     /locus_tag="AC1PIW_000458"
     CDS             complement(20609..21535)
                     /locus_tag="AC1PIW_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HIP6605422.1"
                     /translation="MNLSKEKLLFHHYYANWLRIYKEGAIREVTLKKYRMTLQWLIRL
                     APSLLLEDLNRVRYQELINSYAKTHERQTTMDFHHQLKGAILDAVDEGIIERDPTRKV
                     IIKGRLPRKKKTKYLNQFELHQLIQELRLGEELNWDWLILLIAKTGIRFSEALALTTQ
                     DFDFHRQLLTIDKTWNYKGDGGFLPTKNKSSIRKIQLDWQTSTQFANLLQHTQPDDLL
                     FVKKERIYNSTINAILEKRCLSANLPVVTIHALRHTHASILLFAGVSIASVARRLGHA
                     SITTTQKTYLHIIRELENQDVDLIMRSLSGLC"
     gene            21656..22234
                     /locus_tag="AC1PIW_000459"
     CDS             21656..22234
                     /locus_tag="AC1PIW_000459"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HIP6605423.1"
                     /translation="MKLEELALFMGGSLQVRLDTTSWEDARDYILYNQQHHQLDGYEV
                     IEEEVIDSRTVTTDREVTLLEEGDLLFSLLSGKAVLVRAEHAGLLYTQNYIKIEPIAK
                     LDKAFLLYLINESSAIRRQFYQSLQGSEVMKYTVKQLKSLQLGALPPLDSQQKLGKIY
                     LDGLALRQKRQAYAQGDFLRLTYLLKEVYSHE"
     gene            22227..25433
                     /locus_tag="AC1PIW_000460"
     CDS             22227..25433
                     /locus_tag="AC1PIW_000460"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HIP6605424.1"
                     /translation="MNELAFETELIQYITTGTITQPEHLEGLEWSVSDSSTLYAVKSK
                     LWTYEPGIKTTEQLWENFRNILWQHNQHRLDKPLSDTEFAQVKQIISDLKTPYEAGQF
                     LYGISGVSELEITLDDGRHTFLTVFDQRKIGGGDTVYQVVNQIRRTPKIPGRRECIFD
                     TTLLINGLPIIQIEEKKDTVDVDKALNQMHQYIQERQYTDIFSTLQILVAIAPNNVRY
                     MANTTAEKFNKDFAFHWQRKSDGSKVRNWKDFADSMLSIPMAHQMATNYMILDGTPQK
                     QMLKVMRPYQVYATHAVIEGIKKVDFELGCNKVGYIWHTTGSGKTITSFKTAWLASRM
                     PKVDKVVFLVDRIALTNQTKDDYLAYDPEAGENSFGSIRETQNTTALARQLTSKDSGI
                     IVTSVQKLDTLVKRKSFKSPDKHIVFIVDEAHRSTGGDSFAGIQKAFKKAAWIGYTGT
                     PNLDDTGASLQTQDIFGPLLHAYTIKNAISDRNVLGFKVDFETTIDQEKLKTTYLPDF
                     YRQKYPKWTSAQIEEKIANLSPDDMDDAVTSSVYDENPEHVRLVVEDVFKHWANRSGS
                     GRYNALFTTHVGGGRASTPMAMMYFREFQRINQLHREQGGLVLKVGLTFALNTQNNDS
                     MVESNTGLLEAITIYNTEFGTSFGLSDVAAYTEDLISRLNRTAQDKNYLDLVIVVDQL
                     LTGFNAPELNTLYVDRTLKGASLIQAYSRTNRVEDMDKKPFGRVVNYRWPAHNEKLMN
                     QALAIYADDSAANLSDAEQKNNNEKNGLVAKPFKEVFAEVKQTVDQLRELTGDFNDIP
                     KSEKKREAMLELLRAYNQGLSKLKQYQPEEVDGVIEGFDYNQPDRFIESLGMSVEEEK
                     LLTTVLANELKRTLAKEQKVSIHQIELKMTHIKEVTIDYDKLTELVELLLNQVHEEKM
                     VEAAATRDELDRFASGLEDLNYADQIRQTGDAIYRGDFPTQDMNFAYPVKGIDSQKII
                     QEASHVYIDKKLFAFRIQWGLTEHIRNQELRELLTRHRYGQNDLDDTGHLRTLIKEAS
                     RDYQTWAEGDAVKGLSKIQYRNQLRQALYDLADDLVGQ"
     gene            25541..>26572
                     /locus_tag="AC1PIW_000461"
     CDS             25541..>26572
                     /locus_tag="AC1PIW_000461"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HIP6605425.1"
                     /translation="MTKEKSTVPRLRFPGFTDAWKQRKLGEVADFSIKTNSLSRDKLS
                     SYFYEVQNIHYGDILTKYDAILDVCNKELPSIIGSTISDFADALLSEGDIVFADAAED
                     STVGKAIEVRNFKGKNVVSGLHTIVARPKVSYAPYYLGYLINSTAYHNQILPLMQGTK
                     VSSISKANLKSTTVVFPTLPEQEAIGSFFSDLDQLITLHQRKLDDVKELKKALLQKMF
                     PKGNGNDFPELRFPEFTDAWKQRKLGEFMKESKILGSKGDIARKLTVRLWGRGVVSKK
                     EIYSGSSATQYYIRKSGQFIYGKLDFLNQAFGIIPPELDGYESTLDSPAFDLLKGING
                     QFLLEFVSRK"
BASE COUNT         7718 a         5029 c         6385 g         7440 t
ORIGIN      
        1 ccaaaccttg aaaattgcct agttttctgc tacaatatat tattgactaa aactgaaata
       61 ggatgaaatg tatggtaaca aatgtacttc gttctctcat tgaaaatgat aagggagagc
      121 taagaaaact tgagaaaatg gcagacaagg tcttctcgta tgccgatgaa atggaagcgc
      181 tgactgatga acagttgcaa gctaagacag cagaatttaa ggagcgctac aataacggtg
      241 aatccctcga cgatcttttg tacgaagctt atgcggtagt ccgtgaagga gctcgacgtg
      301 tcttgggtct ctatccctac aaggttcagg ttatgggagg aatcgttctt cacaacggtg
      361 acgttccaga aatgcgtaca ggtgaaggta aaactctgac tgcaacgatg cctgtttatt
      421 tgaatgcctt gtctggtcaa ggtgttcacg tagtgacggt taacgagtat ctttctaccc
      481 gtgatgcgac tgaaatgggt gaattgtatt catggcttgg tctatcagtt ggtatcaact
      541 tagctgctaa atcacccttg gaaaaacgtg aggcctataa ctgcgatatt acttattcga
      601 ccaactcaga gattggtttt gactatctcc gagacaatat ggttgttcgc gcagaagaca
      661 tggttcaacg tcctctcaat tatgccttgg tcgatgaagt tgactcaatc ttgattgatg
      721 aagctcgtac gccgttgatt gtatctggtg cacaaggttc ggaaacaaac cagttgtatt
      781 tcttggcaga taatcttgtt aaatctttga caacagaaga ttacatcatt gacattccat
      841 ctaaaacgat tggactgtct gattcaggta ttgacaaagc agagaaattc ttcaagttag
      901 ataacctcta cgatattgaa aatgtagcca ttacccattt cttggataat gcacttcgtg
      961 ccaactacat catgacctat gatattgact atctggtcaa tgaagaccaa gaggtcatga
     1021 ttatcgatcc atttacaggt cgtacgatgg aaggtcgtcg ttattctgat ggacttcacc
     1081 aagccatcga agctaaggaa ggtgtaccgg ttcaaaacga gtctaagaca agtgcctcta
     1141 ttacctacca aaaccttttc cgtatgtata aaaagttatc ggggatgacg ggtactggta
     1201 aaacagaaga ggaagaattc cgtgaaatct acaacatccg tgttgtacca attccaacca
     1261 accgtccaat cgcgcgtgtt gaccacgaag atttactcta tccaagtcta gaatacaaat
     1321 ttaatgccgt tattgctgat gtgaagagac gctatgagaa agggcaacct gtcttggtcg
     1381 gtacagttgc ggttgaaact tctgatttga tttctcaaaa attggtagct gcaggtgtcc
     1441 ctcacgaagt attgaatgct aaaaaccact atcgtgaagc acagattatt atgaatgctg
     1501 gtcagcgtgg tgcggtcact attgcgacca acatggcggg acgcggtacc gatattaagt
     1561 taggtccagg tgtacgtgaa cttggtgggt tgtgtgttat tggtactgag cgccatgaaa
     1621 gtcgtcgtat tgataaccag cttcgtggac gttcaggtcg tcaaggggac ccaggtgaat
     1681 cacaattcta tctatctctc gaagatgact tgatgaaacg ctttggttca gaacgcatca
     1741 aagtatttat ggagcgtatg aatctaacag aagaagagtc cgttatcaag tctaaaatgt
     1801 tgacacgtca agttgaatct gctcagaagc gtgtggaagg gaacaactac gactcacgta
     1861 aacaagtcct tcaatatgac gatgtgatgc gtgagcaacg tgaaatcatc tatcgccaac
     1921 gccaggatgt tattactgct gaccgtgatt tggctcctga aatcaaagcg atgatgaaac
     1981 ggacgattga acgccaagtt gcgggtcact tccttggttc gaaagatgaa gctattgatg
     2041 gtattatcaa atttgcgcat gcaaatctcg ttgaagatga taccttgagt aaagctactt
     2101 ttgaagcaat gaatcagaaa gagattgtcg aagagctcta tgaacgtgct ttgagagtct
     2161 atgattcgca agtgaaaaaa ttacgcgatg aagaacgtgt acgcgaattc cagaaagttc
     2221 ttatccttcg tgttgtggat aacaaatgga cagaccatat tgatgccttg gatcaattgc
     2281 gcaatgcagt cagtcttcgt ggatacgctc aaaacaatcc aatcgttgaa tatcaatcag
     2341 aagcatttac tatgttcaac gatatgattg gggctatcga gtttgaagta acccgcttga
     2401 tgatgaaggc gcaaattcat gacaatattg agcgtgagcg tacaagtcaa gaagcacata
     2461 cgacagctgt taagaatatt atgccgaacc aatcgcatgc aattcaggag aatgtatctt
     2521 ttgaaggtgt tgatcgtaac gatccatgcc catgccagtc tggtaagaaa tttaaaaatt
     2581 gtcacggccg taaataggaa tgacgcgtgg actggacggc tgtccagtcc ttttgcaatt
     2641 tttagacaag tcactagagt cagttcgttt attttaataa atgaagtgac tatgcaaaag
     2701 gagaccgatt aatgatagta ggacatggca ttgaccttca ggagatggag gcgattgaaa
     2761 gtgccagaat caaacaccaa gggtttccca aaaagattct cacagagaaa gaatttgaac
     2821 ggtatcaaag tctgtccggt cgccgtcagc tagaatattt agctggacgt tggtcagcca
     2881 aagaagcact gaccaaggcc ttggggacag gcattggtaa gataggattt catgatattg
     2941 aaattctgaa taccagcaaa ggagttccct atgtgacgaa gagtcccttt gatgggaata
     3001 tttggctcag cattagccat tcaggaaatt ttgtacaagc tagtgtgatt ttggaggaga
     3061 aagatgattg aaagtgaaca tcgacccacg caaattcggg ttaacttaga tgcgattgcg
     3121 gaaaatttcg agcaagtaat gaccaacttg ccccctaaga ctgaagcatt tgctgttgtt
     3181 aaggcgaatg cttatggtca tggagctgtt gcggtagcga aaaagttatc cagtcaagca
     3241 gctggttttt gtgtttcgaa tttggatgaa gcattagagt tacgtaaagc gggtatcgag
     3301 catcctatct tgattttagg agtggttcct gtacgttttt taccagtagc tcatcaactc
     3361 aatatcagtg tgacagttgc cagtctggac tggttgcaag atgcaaaaga cttagaggct
     3421 gatttatctg gtctaactgt ccatcttaag ctggatacag gtatggggcg gattggtttt
     3481 agaaaaatag ccgatttgct acaagcgatt gctatccttg aagaattaga gtatgacatc
     3541 gaaggagtct atactcattt tgctactgcg gatgaggttg aacaggcgca cttcgagagt
     3601 caactgtctg tttttaaaga atttttggat gttctaccaa ttactccgcg ctggattcat
     3661 gccagcaatt cagcaacaag catttggcat gctgatacag tatttaacct ggttcgtttg
     3721 ggaaacattc tttatggact taatccaagt ggacgagtac tagaactccc gtttggtgtt
     3781 caaccagcct tatctctcgt atctgaaatt gttcatgtaa agcaggttga agctggcaca
     3841 acaattggct atggagctac ttatcacagc accgattcag aatggattgc gaccgttccg
     3901 ataggatatg cagatggtct cgtacgaagc ttacaaggct tatcagtcct agtcgatggg
     3961 caggcctgtg aaattgtagg gcgtatttcg atggaccaga tcaccattcg tttacctcgt
     4021 gcttatccac ttggtcaaaa agtcacccta atcggtcaag atggagataa aacgatctct
     4081 gttcaggagt gggctgatcg catcggaacc ataaattacg aggtggtttg tcttttgaca
     4141 gatcgtctac ctcgaatttt tgagtaaacg tgatgataat ccgcttcttt cactaaaata
     4201 gaaggctaaa atattagaga agtgttgcct agagattgtc tataatacaa atatcgtcat
     4261 ttagttgcct tttattactt tgacaatcga ggaaatatcg tttccatatt tccaacttca
     4321 aacactccac cggattgttt gaactcgcct tgcctgattt tgtaaaagtg acttgctttg
     4381 caagtattat ctcccacctt gcacagtcca ttgactgtgc aagcaatctg ggggatacga
     4441 atagtccagt ggactattcg tacctgagcc tgaaaacaaa aaagcgaagt acatcagctg
     4501 gtcacctaga aataagaaag cattgagtta tgcctgtggc ttttgatgag gctgggcaaa
     4561 aagtccagga ccactactca gagttcgtgt caacatctca gcgcagtggt tgattggcat
     4621 atttgttcgt gttttgcact ccaaatctgg cctaatcaac tgtgcggggg gtgggaagac
     4681 gaactctttt tatcttggtc gagttctttc ccactccctt tgtccccagt ctccaactat
     4741 ctcccagata gttggagcga gtactaaaag taaactaaaa gactataatg aggctggtca
     4801 aaaagtccaa tactcttcga aaatcaaaat tatccgttgt caacttgcct tgatgaactc
     4861 cagttctatc ttcgacttcg tttcctaggc tacttttgat tttcattgag tataaaaaat
     4921 aaaaaatagc ttagaattgc atcgtcagaa acgttgattc taagctattt ttgtttattc
     4981 aaattttaat tggtactcaa tgaaaattaa aatcagacta gctccaatgg agcttgacac
     5041 tcatcaaatc aagtcaacaa cgtctggttt tgattttcga agagtatgaa gcgacctcgt
     5101 tgtgattatt gctcgttaaa aaaagcttca tagagtgcgt gcagggcgcg tttcttttct
     5161 tcggttttga caacgaacat aacagaaact tcactggctc cctgtgaaat cattgccaag
     5221 ttgacgtttt gtttagagag ggcagtggta gcggtagctg ttacaccgac atggctcttc
     5281 atattttcac caacaatcat gataatagat aaaccatgtt cgatttcggc cttgtcaact
     5341 tccattttcg tattgagttg acgaaggatc tcttcttcct tgataggagt caattcacgc
     5401 tcacggacta cgatggataa atcgtcaata ccggtcggca tgtgctccca gcgaatattg
     5461 agatcttcga gaatttgcaa taccttgcga ccaaaaccaa cttcacggtt catcaagtat
     5521 ttggacaagt taatgctgac aaaaccatcg tctgcagcaa taccgacaac aggaactgtt
     5581 tgctcggtat gtttgtgtac aattcgggta ccagggtgag ttgggttatt ggtgtttttg
     5641 ataaccaatg gaattcgtcc gcgataggca ggaaggaggg cttcatcgtg gaggacggag
     5701 aaacctgcat aagctaattc tcgcatttca cgataggtca attctttgat tgaatgtgga
     5761 tttttaatga tgccaggatg ggctgcaaaa ataccatcga catctgtaaa gttttcatag
     5821 aggtcagcct tgaccccggc agcaatgatg gaacctgtaa tgtccgatcc acctcgtgag
     5881 aaggtacaaa tctggttatc aacagttaca ccgaaaaatc ctgggataat gagtacttca
     5941 tctgtatcac gaagctcttc gattttgtca taacttgaag gcaaaatgcg agcatttcct
     6001 ggttcggagc tgacaataat accagctttt ttaggatgga catagcgtgc aggcaatcct
     6061 ctgtgggtaa aatattcggc aatcaatttg gcattattat cttcaccagc tgctaagaat
     6121 gcgtcgtaaa ggaattcgtt atcatcaatt ggtaggctgg ccaaagagat gatgctgtct
     6181 gcgattttct tcatgctgtt agctgtgaaa ccaagttcgt caaccattgc ttgataacgg
     6241 ttgatgatcc attcttgact ggcagtcaca tcttttccgt taacatattc tttataatat
     6301 ttaatcaacg catcagtaac tttagtatcc tcggcattgc gttttccagg tgcagacacc
     6361 acaacaaagc gacgttctga atcagattga acaatattga aaactttttc taactgtcca
     6421 gctgaagcaa gtgagcttcc cccaaattta ataactttca ttctcatctc cttggatatt
     6481 tttttctatt atagcagaat gatatgaaat tgtcagtatg ttcagacaaa tcttgccaag
     6541 aattttatcg aaaaacgaac aagagacaag tatgaaaatt tcagaaaaac atttttttct
     6601 ttcaactgtg ttacaattaa agtaatagga ggcgagtatg gtaaaagcaa ttattttcga
     6661 catggatggt gtactttttg atacagaaac attctatttt caacgtcgag cggatttttt
     6721 agcgacaaaa ggtttatcgg ttgatcattt ggatccatct atatttgtcg gcggaagagc
     6781 tagtcaaatt tggcaacgga ttttagggga tgattatgat aactgggatg tccctgcctt
     6841 ggaagaagaa tatcgtgtgt ataaagaaaa gcgaccaacc ccttatgcag agaggatttt
     6901 tcctgaagtt aaagcggtat tggagagact aactatccaa ggtattcctg tagttttggc
     6961 ttcaaatacc gaccgttcag aaatcgaacg tgctctacta gatgctggta tacgctttca
     7021 ctttagtcat gttttctcag cgatggattg tgaggcccca aagccagatc ccacagttta
     7081 tatgaaagca gctagtgcaa caggagtagc taaatcggac attttagtat ttgaagatag
     7141 tgcgaagggg attgaagcgg ctaaaaaagc aggtttgaca gtttgggcta ttcgagacca
     7201 gcattatgga attgatcagt cgaaagcaga caaattggta gacaatcttg aagatgcctt
     7261 tgacttattg cgattttaag aaatattata ttgtagtcca agcatcagtt ctcgtgaaaa
     7321 atggtatttc tagctcagaa tccagttaaa tcttccaaaa gtattaacgt gaaaaaatga
     7381 aagttgtaaa ttaaacttag agcctataat ttacagttca gacttgcata ttgaggttgt
     7441 tttttcaaaa tacagtcagt atttcccgca aaactacctt gatgagtgaa aaagccaagg
     7501 cgctttgcgt gtgaatatag attcttagac acgttatttt ctagcatatc taaattttat
     7561 attgcgatga tggcctccat ctttttcgga cgtcacactt tccttgctag aaaaaagtga
     7621 caattttaaa aactatgtta caatggaaaa caagaaattg aagtttgata ttcaaatctt
     7681 ttaattatca aactattttg aaaggagcaa gagaatggcc tatcaaacaa ttcgttatga
     7741 agtaattgat tccgtagcga ttctaacttt aaacagaccg gaagtggcaa atggttttaa
     7801 tattcctatg tgtgaagaaa tcttagaagc aatccgcctt gcagaagggg atgcctccgt
     7861 caaaatttta caaattcaag cacagggttc cattttctcc gtgggtggag atttagttga
     7921 gatgaagcgt gcagttgatg atgatgatat tgcttctttg gtaaaaatag cagagctggt
     7981 gaatgatatt tcgtttgcga tgaagaaatt gtctaaagta gtcattatgg tgacagatgg
     8041 ggcagtggct ggagccgcgg ctaatatggc agtggcagct gattttgtta ttgcctccag
     8101 caagacaaaa ttcatccaag cctttgtagg cgttggactt gcgccggatg cgggggggct
     8161 ctttctactg agtcgtgcta ttggggctaa tcgtgctagt cacttggcga tgactggaga
     8221 gggcttatca gcggataagg ctttagaata tgggattatt tataaattgg cagagccaga
     8281 aaaactagaa aagacggttg cccaggtcat aaaaaaactc ttgcgaggct cagtaaattc
     8341 ttatgcagca atcaaacaat tgacatggga aagtcaattt aaagactggg agtcttatcg
     8401 ccgattggag ctggatttac aagaaacatt agcctataaa gaggatttta aagagggagt
     8461 tcgagcgcat gcagaacgac gtagaccagt gtttacggga gattaatttt taaattagta
     8521 aaaaatgttt gacaatcaaa gttttatggt ttatactttg actatcaaag taaaggaggc
     8581 ctgttttgga agatccaaaa gtaaatgaat atctaacggc catttttaac aatgtgttgg
     8641 tgattgaaga aacaagtctg cgtggtagcc gattcaatga tatttcgatt aaggaaatgc
     8701 acacgattga tgtgatagga agtattgatg gagctactcc gagtgatgtt gctcggactt
     8761 tgatggtaac cttaggtact gttacaacta gcttgaacaa tttggaaaga aaaggatatg
     8821 ttgagcgtat tcgttcgacc aaagatcggc gagttgttca tctctatctg accaaaaaag
     8881 gtcgactggt ctatcgtttg catcaacgat ttcacaatga aatggtgaaa cagattacag
     8941 atggtatgga tgatgcagag tatcaggtaa tgaagaaagg actcatgaaa ctctatcact
     9001 tcttggagga tttgaaatga gaaaccatgc taaaatcagt caggtggccc actatcttcc
     9061 gaagaagatt gtgaccaatg atgatttggc tcaacgaatg gagaccagtg atgagtggat
     9121 tcgtagccgt acaggtatcg gtcaacggca cattgttact ggagagacga cgagtgattt
     9181 ggctagtcaa gtggctagaa aattactgga aaagtctcag ctagatgcta gtgaaattga
     9241 cttcatcatc gtagcaacga ttactccaga tgcttcaatg ccatctacag cagctatggt
     9301 tcaagcagca attggcgcga aaaacgcttt cgcctatgat ctcgttgcag cttgttcagg
     9361 atttgttttt gcgctatcaa cagcggaaaa attgcttgct tcaggagtct ataagagagg
     9421 acttgtcatt ggtgcagaga ccctatctag gtctgtcgac tggtcggatc gatcaacggc
     9481 tgttttattt ggagatggtg ctggaggagt tttgctggaa gcgtgtgaac aaccgacctt
     9541 tttagcagaa atcttgcgaa cggatggcgg tcgaggtgct agtctgacag cagggataga
     9601 ccagaaagag acgccattct cgacacaaag ctgtcagcaa ccctttatcc aaatggaggg
     9661 cagagctata tttgaatttg ctacgaggga tgttacggca accatggcag agctgctaga
     9721 acaagctgat atgactgtgg attgcgttga ttatttcttg ctccaccaag ccaatatacg
     9781 tatcctggat aagatggctc gtaaattggg agtggcgcgt gaaaaatttc cagcaaatat
     9841 ggataaatat ggcaatacca gtgcagcaag tcttcctatt ctcttatcag aatgtgtaga
     9901 gagcggaatg ttgcgattgg atggaagcca gactattctc atggctggtt tcggtggagg
     9961 cctcacatgg ggaactctac tcttacaact ttagttaatt ccttgcccta ggcaagatta
    10021 aaatatttta ttttacaaaa ggagacacat atcatggcag tatttgaaaa agtacaggaa
    10081 atcatcgttg aagaattggg taaggatgct gaagaagtaa ctcttacaac aacctttgag
    10141 gatttggacg cagattcatt ggatcttttc caagttatct ctgaaatcga agatgcattc
    10201 gacatccaaa tcgacactga agaaggtttg actactgtag gcgatttggt tgcttatgtt
    10261 gaagaaaaaa caaaataatg actgttaagg agagtgctat gtagcatttt tcttaagtta
    10321 aatactttga taatcaaaat attgaaatat caaactattt tttgagtgag ggggggcaac
    10381 ccttcacttg tttacctaat gatttgatat tcaaatgatt aggtaagcaa gaaaaaaaga
    10441 tgagggaatt atgaagacga aaataacaga attgctcgat attaagtatc caatttttca
    10501 aggtggtatg gcttgggtag ctgatggaga tttggctggt gccgtatcta atgcaggtgg
    10561 tctaggaatc atcggtggag gaaatgctcc caaagaggtg gtcaaggcaa atattgacaa
    10621 ggttaaatct atcacggaca aacctttcgg tgtcaatatc atgcttttgt ctccttttgc
    10681 ggatgacatt gttgacttgg tcatcgaaga aggtgtgaaa gttgtcacca caggagcagg
    10741 aaatccaggg aagtatatgg agcgtctcca tgctgcaggg attacagtta ttcctgttgt
    10801 accgagtgta gcccttgcta aacgcatgga aaaattaggg gtagatgctg ttattgctga
    10861 aggtatggaa gccggaggtc atattggtaa attgaccacc atgaccttgg ttcgtcaggt
    10921 tgttgaagct gtatctattc ctgttattgc tgctggaggt attgcagatg gtgcaggtgc
    10981 agcggcagcc tttatgttgg gagcagaagc ggtgcaagtt ggaactcgtt ttgtagtagc
    11041 tacagagtcc aatgcccatc aggcttataa ggaaaaagtt ttaaaagcta aggatattga
    11101 tacaactgta tcagcttcta ttgttggaca tcctgtgaga gccatcaaga ataagttgtc
    11161 atctgcttat gcagctgcag agaaagattt cttggcaggt aaaatttcgg cagatgctat
    11221 tgaagagctt ggtgcgggag cacttcgcaa tgcagttgtt gatggtgatg taacaaatgg
    11281 ctccgtaatg gctggtcaaa ttgctggtct agtttctaaa gaagaatctt gtgaagatat
    11341 tttgaaagat atctattatg gagcagccaa agtgattaga gaagaggcaa gccgttgggc
    11401 ttcggttgga gaataggatg acaaagacag cttttctttt tgcaggtcaa gggtctcaga
    11461 cagtggggat ggctcgcgac ctttacgaat attacccgat agttagagaa acgtttgacc
    11521 aggctagtca gattctgggg tatgacattc gcgacctaat tgacaatcaa gaggataaat
    11581 tacatcagac tcgctatacc caacctgcta ttttgaccac ttctgtggct atttttcgac
    11641 tgctggaaga agagggaata caacccgaca tggtagctgg tctatccttg ggagaatatt
    11701 cagctctggt ggcatcggga gctttggcct ttgaagatgc gattgccctg attgctaagc
    11761 gtggtgaata tatggaactg gctgcgccag cagggactgg taagatggtt gctgttctca
    11821 acacagatgt caacttgatt gaagaaatct gtgcgactgt tacctctggg attgtttccc
    11881 cagccaacta caacacacca gcccaaatcg tcatcggagg cgaggtggct gcagtggatg
    11941 aggcggttga attgctgaag gaagcaggtg tcaagcggat gattccgctc aatgtatctg
    12001 gcccctttca cacggctctc ctgcgaccag cgtcggagca attggctcag gccttggagc
    12061 aggtggaact tgcggacttc caagtagagt tggtcggtaa tacggaagcc gaggttatga
    12121 aaaaagagga catcaagtcc ctcttgcccc gtcaagtgat ggaaccagtt cgtttctatg
    12181 agtcaatcgc aaccatgcag gaagctggtg tgactaactt tattgaaatc ggtccaggta
    12241 aagtcttatc aggatttgtc aagaaaatcg ataagacggc gcatgttgta gcaattgaag
    12301 atatagaggg gctacaaata gtattaaaca aatcatgagg aggaatcatg gaattaacaa
    12361 ataaaaatgt ttttgtaaca ggttcaagtc gaggaatcgg tttggctatt gctcataaat
    12421 ttgccagttt aggagcaaat gtcgttctta atggtcgtgg tcagctaggt caggacttgc
    12481 tggatagctt tgcagattac ggtgtgaaag tcctagctat ttcaggagat atttcaagcg
    12541 cgcaagatgc caaacgaatg gtggcagaag ccatcgaaac actcggtagc gtcgatattt
    12601 tggtcaacaa tgcagggatt accaaagatg gaatggctct tcgtatgaca gaagaggatt
    12661 ttgatacggt tctgaaggta aatcttactg gaacttttaa tatgacgcag gctgttttga
    12721 aaccaatgac aaaggctcgt gagggtgcta ttatcaatct atcaagtgta tctggcttga
    12781 ttggaaatgc tggacaggcc aactattctg cttctaaggc aggtgtgatt ggatttacta
    12841 aagccattgc ccgtgaggtt gctgggcgaa atgttcgtgt caatgcgatt gcgccgggct
    12901 ttatccaatc tgatatgaca gatgttttat ctgacaagat taaagaagct atgactgcac
    12961 aaattccgat gaaacgcttt ggtgcgacag aagaagtagc tgatgttgca gtgtttcttg
    13021 ccaagcaaga atatctaaca ggacaggtga ttgcggttga cggcggcttg accatgcagt
    13081 aagtttccta atgagaaagg actgaaaatg acaaaattaa atcgtgtagt agtaacaggc
    13141 tacggtctga catctccaat cggaaatacg ccagaggagt tctggaatag tttgaaggct
    13201 gggaaaattg ggatcggaaa gattaccaag tttgatacca gtgaatattc ggtccataat
    13261 gctgcggaat taaaagattt tccttttgac aaatatttcg tgaaaaagga tacaaatcgc
    13321 tacgataatt actcgctcta tgcactctat gcagctaaag aagcgattgc taatgcacag
    13381 ctggatacag agacagtgga tagtgaccgt tttggtgtta tcttatcaac aggtatcggt
    13441 ggtattttgg aaattgaaga gcaagttgct cgtatgaatg aaaaaggtcc aaaacgcatt
    13501 cgtcccatgg ctcttccaaa ggccctgcca aatatggcgg ctggaaatat tgctatgcag
    13561 gtcggtgcaa atggcgtctg caagtgcatc atcacagcct gcgcttcatc aaatgatgct
    13621 ttaggggaag ccttccgtga aatcaagttt ggtttccaag atgtgatgct ggctggcgga
    13681 gcagaggcag ccattactcc gtttgcaatc ggtggtttcc aagctttaac agctatgtcg
    13741 actactgagg atccagaacg tgcgtctatt ccatttgaca aggaccgcaa tggttttgtc
    13801 atgggagagg gttccgcggt tttagtattg gaaagtttgg aacacgcaga ggcgcgtgga
    13861 gcgacgattt tggcggaaat cgttggttat ggaaatactt gcgatgctta ccacatgact
    13921 tctccgcatc cagaaggtct gggtgctatt aaggccatga agttggccat ttcagaagca
    13981 ggtttagagc cagctgatat tgactacatc aatgcccatg gcacttcgac accggctaat
    14041 gaaaaagggg aaagccaagc tattgtatcc gtattcggca agaacacgcc agtttcttcc
    14101 accaagtcct tcacagggca cttgttgggg gcggcgggtg cagttgaggc ggcagctgtc
    14161 attgaggcta tgcgtcattc ttatgcacca aagacagctg gtacgacaga attatctgaa
    14221 gatattgaag cggatgtcat ttatggacag gggcgtgata tggaaatccg tcatgccatt
    14281 tcaaatacat ttggctttgg tgggcataat tcagtcatcg ctttcaaacg ttgggaggcc
    14341 taagtgaata ttacagaaat taaggacctg atgagtcagt ttgaccagtc aagtttgcga
    14401 gaattttcgt atagcaatgc tggggagatc ttgcatttca gtaaaaacca gcaggcgaca
    14461 gtggctccga gtccagcggt agaagctccg cttgccccag taactcctgc aagcccagcc
    14521 cctgtgccag cagttgaaat gagtgagcca gctccagcag aagcaggtgc cagccccgtg
    14581 gcagaaggtg cggtagtgga aagccccttg gtcggcgtgg cttatttgtc cccagcccct
    14641 gacaagccag cctttgtggc agttggggat acggttaaaa aaggccagac cctcatgatt
    14701 atcgaagcca tgaaggtcat gaacgaagta ccagctgacc gtgacggtgt ggtcacagag
    14761 attttggtgg ccaaccaaga tgttgtagaa tttggacaag gattggtacg catcaaatga
    14821 ttgatatttt gaaaattaaa gaggcgcttc ctcatctcta tcctatgctc ttggtcgatc
    14881 gtgtccttga agtatcggaa gatgagattg ttgccctcaa aaatgtgacc attaatgagc
    14941 ccttcttcaa tgggcatttc ccagactatc ctgtcatgcc aggtgttctc atcatggagg
    15001 ctttggcaca aaccgcaggt gtcttggagt tatccaagga agaaaacaag ggcaagctgg
    15061 tcttttatgc cggtatggac aaggttaaat ttaagaagca agttgtacct ggtgaccagt
    15121 taatcatgac cgctaaattt gtcaaacgcc gtggaaccat tgcggtcgtt gaggcaaagg
    15181 cagaggtgga aggaaaactg gctgcgtcgg ggacgttgac ttttgctatt ggaaaataat
    15241 actcttcgaa aatcaaactc agccgttgtt gacttgattt gatgagtgtc aagctccagt
    15301 ggagcttgac agcctgctac cataagataa gaaagtagca ggagtatatc tgctcttgga
    15361 gaaacgaact tcgttcgctc tatctccaac ctccaaagct ttcccaaacc tttggagcta
    15421 gcctgatttt gattttcatt gagtatgaat cggtccgtta ttttgcaaac tagttaaggg
    15481 ctgagcttgt gataaataat tattctatgt taaaaaggag ataggattca tcatgtttga
    15541 gaagattttg attgctaatc gtggtgagat tgcggtgcgg atcattcgtt cggccagaga
    15601 gttggggatt gcgacggtgg ctgtctattc agaggctgac aaggaagctc tccacaccat
    15661 gttggcagat gaggctgtct gtatcggccc agcccgttcg acagactcct atctcaatat
    15721 gcaggcagtc atctcggcgg ctgttgtgac aggtgcccag gccattcatc ctggctttgg
    15781 atttttgagt gaaaactcca aattcgccac cctctgtgaa gaggttggta tcaagttcat
    15841 cggaccttct gggacggtta tggataccat gggcgataaa atcaacgcgc gggcggagat
    15901 ggttaaggct caagtgcctg ttattccagg ctctgacggt gaagttttga ccagtcaaga
    15961 agcccttgaa atcgctgaaa agattggcta ccctgtcatg ctcaaggcat cggcaggtgg
    16021 tggcggtaag gggattcgta aggtggaaaa agctgaagac ctagtgcctg ccttcgaatc
    16081 ggcttccagc gaagccaaag cagcctttga taacggagct atgtatatag agcgtgttgt
    16141 ctatccagct cgccatatcg aagtacagat tttggcggat cagcatggtc atgtcattca
    16201 tttgggagaa cgggattgtt cccttcaacg caataaccaa aaagttttag aagaagctcc
    16261 gtcaattgct atcggtcaga ccatgcggga acgtatcggt caagcagcag tgcgggctgc
    16321 ccagtcagtt ggatatgaaa atgcaggaac cattgagttt cttttggacg aagccaaggg
    16381 cgaattttac ttcatggaaa tgaacacacg ggtgcaggtg gaacatcctg ttaccgaatt
    16441 tgtcaccggt gtggacattg tcaaggagca aatcaagatt gcggctggtc aggaactcag
    16501 tgtccgccaa gaagatgtga agattactgg tcatgccatt gaatgccgca tcaatgctga
    16561 aaatccagcc ttcaacttcg ccccaagtcc aggtaagatt tccactctct acctgccaag
    16621 tggtggcgtt ggtttgcgtg tagattcagc tgtttatcca ggctacacca ttccgcctta
    16681 ctatgattct atgattgcca aggtcatcgt gcatggggaa aatcgttttg aagccctgat
    16741 gaagatgcag cgagccctct acgaattgga aattgacgga gtggtgacca atacggactt
    16801 ccagctggac ttgatttccg acaagcgagt ggtggctggt gattatgaca ctgcctacct
    16861 catggaagaa tttttacccc gctatcaaga agatctaaaa aagtaaacta ggagaatgct
    16921 atggctttgt ttcgcaaaaa ggacaaatat atccgcatca atcccaatcg ttcgcggata
    16981 gagtcagcac ctcaagcaaa gccagaggtg ccagatgaac tcttttctaa atgtcctgct
    17041 tgtaaggtca tcctttacaa gaatgattta ggattagaga aaacctgtca acactgttcc
    17101 tataattttc ggattacagc ccaggaacgc cgggctttga cagttgatga aggatcgttt
    17161 gaggaattgt ttacagggat tgaaacaact aatccgctgg acttccccaa ttatttagag
    17221 aaattagcag ccacccgtca aaaaacgggc ttggatgaag ctgttttgac aggcaaggcg
    17281 acaatcggcg gtcagccagt tgcccttgga atcatggatt ctcactttat catggcttca
    17341 atgggcacag tggtcggtga gaaaattacc cgcctctttg agttggcgat tgaagagcgt
    17401 ttgccagtcg tcctctttac cgcatctggc ggtgctcgga tgcaggaagg catcatgagc
    17461 ctgatgcaaa tggccaagat ttctgcagcc gtgaaacgcc attccaatgc tggcctcttt
    17521 tacctgacgg tcttgacaga ccccacaaca ggaggtgtga cagctagttt tgccatggaa
    17581 ggcgatatta tcctagctga gccgcagacc ttggttggtt ttgcaggacg acgggttatt
    17641 gagtcaaccg ttcgggaaaa tctgcctgac gatttccaga aggctgaatt tttacaggaa
    17701 cacggctttg tcgatgctat tgtcaaacgc caggacttgc cagcgaccat cagtcgcttg
    17761 ctgagaatgc atggaggtgt cagatgacca gtgatgtagc acgtatgatc cgtttggcac
    17821 gtgaccaagc tcgcctgacg accttggact atgccacaca actctttgat gactttatcg
    17881 aattgcacgg tgatcgcaat ttccgtgatg acgaagctgt agtgggtggc atcggctttc
    17941 tagcaggtca gccggtgaca gttatcggta tccaaaaagg gaagaacctt caggacaacc
    18001 tcaaacggaa tttcggtcag ccccacccag aaggctaccg caaggccctt cgccttatga
    18061 agcaggcgga aaagtttggt cgccctgttg tcacttttat caataccgcc ggagcctacc
    18121 caggcgtcgg tgctgaggag cgtggacagg gcgaggccat tgcccgcaac ctcatggaga
    18181 tgagtgacct caaggtgcct attatcgcta ttatcatcgg agagggcggc tctggtggag
    18241 ctctggctct agcggtggca gaccaggtct ggatgctgga aaactccatg tacgccgtcc
    18301 tcagccccga aggtttcgcc tctatcctct ggaaagacgg cagtcgagcc atggaagcgg
    18361 cagaactcat gaagatcact tcttatgagt tggagaaact ccaagtggtc gatagggtgg
    18421 ttttggaaaa tggcagagcg accaaggaag tcttgtctga tatcaaggac aacctggtca
    18481 gtcagttggc tcaactacaa gccttaccac ttgaacaact actcgaaaac cgctatcaac
    18541 gctttagaaa atactaggaa gacctagtat ttttttgata gatttgatac aatggataaa
    18601 ataatttcag gaggttttcc atgttagtaa aagcagatct atcaaacgca gaagaattgc
    18661 tacctattca gcaccgagca tttgcggctt tatataaaac ctatcaggac cagtacaacc
    18721 ctgccattga aactatggac tatttccaat cacgctttgc acgaccaaat tgtagctatt
    18781 acaagattat cgaggaagag cacacagtcg gactagtacg gacaacggtc gctgaagacg
    18841 ctgaacaggg ctggctaggc ttgattggta ttgatccaga ccacagagga aaaggctatg
    18901 gtcacaaggc tatgctcgaa ttggaaagcc tttatccgac agttagacgc tgggttctct
    18961 gcacggtttt gcaggagtct aaattagttt ctttctacga aaagctcggc tacaaggcca
    19021 tcaaaacaga gcctgaacaa gaaggcatgg atatggtcta tatggagaag tggataggag
    19081 attcagatgc ttgaacagtg tcgcagatac ctggattatc aaggagttca ttattgcaag
    19141 cctgaaaaag caggagcttt ggcaggcgat atgatagcct taaaaaatgt agcacaggca
    19201 gcacgcaagg agtttcagac attaagttta gtcttagctg agcacgtcag tccctttgtg
    19261 cctgagaggg tcagtcaatg gatgaatcaa gcccaaattt gcagaccaca tttttggacc
    19321 tattaccgtt ttccctctgc tagtctaacg gactcggctc ttgctattcg tttgtatggt
    19381 aagggaactg aagcagggat ttcagtagaa gtcagtttta ttgaacgaaa gaaagtggag
    19441 acctctctgc ttcagcaaaa tcgtgttctg aactgctcga ttgcggcacc gctttattat
    19501 tgggtgcaag agggacaggt attttatcgc ctggacggaa cggaggagaa tagacagagc
    19561 ctattacaag ccgttgaagc aggacggatt cgtaaagtat tggtcaagta tgatgtaccg
    19621 tttagaaccg ttgcctccga ggaggaactg ctggaacgct tgattcaggg cttttctcta
    19681 ctagagccct acttgaagat gacacacctc gctaaataat cagtggggtg atggcaacaa
    19741 acatcgtaag ggtatttgtt ttttagtgta tattgacaaa ttcattgaat ttttgtaaaa
    19801 ctgtatcaca acaacagaac agtttgattg acattacgtg aaatcctata atagattgaa
    19861 ggcactctat atgatgaagc ggaagaggag aagagtatgg agaaactagg tcaaagtttg
    19921 aaatggcttg gtcggaagat ttggctatta ttaaggattt ttggtctctt agtgcttgct
    19981 gtccttccgg atctgatttt ggaagggtcc aaatcgcaag tcaccttggt aatagttggg
    20041 ctgatgacac ttggactttt agtctttttc tggtggcggg ctgaaaaaat gatctgccag
    20101 tctgggaccc taacattctt acttgggacg gtctggcgat agttatatta gcctttgcta
    20161 tcatgcagtt gacggatatg cttggttatt atttgttgga attgcagggt gttaccacaa
    20221 cagaaaacga tgcttcgatt atagaatttt tgaagggagt tccttttaga ctggcactct
    20281 tgacaacagc atttttgcca gcagttgtag aagaagtcat atttcgaggc tacttcttta
    20341 agaaattatt tggatcacag gttttgttag gaatagttgt ttcaagcctc gtttttggct
    20401 cctttcatgg tccaacagac ttaggctctt ggttgattga tgctggcagt ggtattattt
    20461 tgtctctcct ctattacaag agtagatact taatctatcc aataatcgta cacttggtga
    20521 acaatttcat tgcaacagtt ttttactata tataagaaaa gacggttcca tacggattcg
    20581 tcttttgcta taagtagaaa catggaattt aacaaagtcc ggagagtgac cgcataatta
    20641 aatcaacatc ttggttttcc aactcacgaa tgatatgaag ataggttttc tgggtcgttg
    20701 ttatgcttgc atgaccaagt cgtctagcaa cacttgcgat agagaccccg gcaaataata
    20761 aaatagatgc atgggtgtgt cgcagggcgt gaatggtgac gacaggaaga tttgcactta
    20821 ggcaacgctt ttcaagaatg gcattgatgg tagagttgta aatccgctct ttcttcacga
    20881 aaagtaggtc atcaggttgg gtgtgctgta ataagttggc gaattgggtg ctggtttgcc
    20941 agtcgagttg gatttttcga attgaagatt tattttttgt aggtaaaaat ccgccgtctc
    21001 ctttataatt ccatgtttta tcaatggtca atagctgtcg gtgaaaatca aaatcttgag
    21061 tagtgagagc aagggcttcg gagaatcgaa tacctgtttt agcaatcaat aagatgagcc
    21121 aatcccaatt cagctcctct ccgagacgta attcttgtat caactgatga agttcaaatt
    21181 ggttgaggta ttttgtcttt ttctttcgag gaagtcttcc tttgataatc accttacgtg
    21241 tcggatctcg ttcaataatt ccttcatcta cagcatctaa aatagctcct ttcaactggt
    21301 gatgaaaatc catggttgtc tgtcgttcat gtgttttagc atagctgtta atcaattctt
    21361 ggtaacgaac ccgattcaaa tcttccaaca acagactagg agctagcctg ataagccact
    21421 gcaatgtcat tctatatttc ttgagagtga cttctcgaat ggctccttcc ttgtaaatcc
    21481 gaagccaatt tgcataatag tgatggaaaa gcaatttttc ttttgataag ttcatggcag
    21541 aacctccttt tttaattttt gtaaattaca taatttattg acaggtttct gagaagatgc
    21601 tatacttagt ttatcaaaaa atgtaattat gaaaaatttc aaagaaaggg aaattatgaa
    21661 actagaagaa ttggctctct ttatgggagg tagtttacag gttcgcttgg atactactag
    21721 ctgggaagat gctagagact atatcctcta caatcagcaa catcatcaat tggatggtta
    21781 tgaggtgata gaagaagaag tgatagatag tcggaccgtg acgacagata gagaagtgac
    21841 cttgctcgag gagggagact tactctttag tttactatcg gggaaggctg ttttagtgag
    21901 agcggagcac gcaggcttac tctatacaca gaactatatc aaaattgagc ccattgcaaa
    21961 gctggataag gccttcttac tgtatctgat caatgaatcc tcggctatta gacggcagtt
    22021 ttaccaaagc ttacagggga gtgaggtaat gaaatataca gtcaagcaac tgaaaagttt
    22081 acagctaggt gccttgcctc ctttagatag tcagcagaaa ctaggaaaga tttatttaga
    22141 tggattagcc ttacggcaga agcggcaagc ttatgcgcaa ggagattttt tacgactaac
    22201 atatttgtta aaggaggttt actcacatga atgaattagc atttgaaaca gaattgattc
    22261 agtatattac gacagggact attacacaac cggagcacct agaaggttta gaatggtctg
    22321 ttagtgattc ttcgaccttg tatgcagtca aatctaaact gtggacttat gagccgggta
    22381 tcaagacaac ggagcaatta tgggaaaatt ttcgtaatat tctctggcaa cacaaccaac
    22441 atcgattgga caaacccttg agcgataccg agtttgctca agtcaagcag attatttcag
    22501 atctgaagac accttatgag gctggccagt ttttatatgg aattagcggt gtttccgaat
    22561 tggaaattac gcttgatgat ggacggcata catttttgac agtattcgac caacgtaaga
    22621 taggcggtgg ggacactgtc taccaagtgg tgaaccaaat tcgtaggact cctaaaatcc
    22681 ctggacgacg agaatgtata ttcgatacaa ccttgttgat taatggctta cctattattc
    22741 agattgaaga gaaaaaggat acggtagatg tggacaaggc attgaaccaa atgcaccaat
    22801 atatccagga acggcaatat acggatattt tttctacctt gcagattcta gtcgctatcg
    22861 cccccaacaa tgttcgctat atggccaata ccactgcgga aaaatttaat aaggactttg
    22921 ctttccactg gcagcgtaaa tctgacggta gtaaggttcg gaattggaaa gactttgcgg
    22981 attccatgct cagcattcct atggcccatc agatggcgac caactacatg atcttagacg
    23041 ggacaccgca gaaacaaatg ctgaaagtca tgcgtcccta tcaagtttac gccactcatg
    23101 cggtcattga ggggataaaa aaagtcgatt ttgaactagg ctgtaacaag gttggctaca
    23161 tctggcatac gacaggctca gggaagacga tcactagttt taaaacagct tggctggcaa
    23221 gtcgaatgcc caaggtagac aaggttgtct ttctagtgga tcgtatcgca ttgaccaatc
    23281 agactaagga tgattatctg gcttatgatc cagaagctgg ggagaatagt tttggcagta
    23341 ttcgagaaac tcaaaatacc acagccttgg cacggcagtt gactagtaag gatagtggga
    23401 ttatcgttac ttctgtgcaa aaattggata cactggtgaa gagaaaatcc ttcaaatcac
    23461 cagataagca tatcgtgttt atcgttgacg aagctcatcg ctcaactggt ggagatagtt
    23521 ttgcagggat tcaaaaggcc tttaagaaag cggcttggat tggctataca ggaactccca
    23581 acttagatga tacaggtgct agtctgcaga cgcaagatat ttttggccct cttcttcatg
    23641 cctacactat taaaaatgcc attagtgacc gcaatgtact agggtttaag gtagattttg
    23701 agacaaccat cgatcaggaa aaactaaaaa caacttactt accagatttt tatcggcaaa
    23761 aatacccgaa gtggacaagt gcacagattg aagaaaaaat tgccaatcta agtccagatg
    23821 atatggatga tgcggtgact tctagcgtct atgatgaaaa tccagagcat gttcgattgg
    23881 tagtcgaaga tgtcttcaaa cactgggcaa atcgctctgg cagtggtcgt tacaatgcct
    23941 tgttcaccac ccacgtaggt ggaggcaggg cctccacacc catggctatg atgtatttcc
    24001 gagagtttca acgaatcaat caactacatc gagagcaagg aggtctggtc ttgaaggtgg
    24061 gattgacctt tgccttgaat actcaaaata atgatagcat ggtcgagtca aatactggtt
    24121 tgcttgaagc tattacgatc tataacacag aatttgggac gtcctttggc ttgtcagatg
    24181 ttgctgcgta tacagaggac ctgattagtc gcctcaatag aacagctcag gacaagaact
    24241 acttagattt ggtgattgtc gtggatcagt tattgacagg tttcaatgct cctgagctca
    24301 atactcttta tgttgaccga acccttaaag gagccagtct tattcaggcc tattctcgta
    24361 ccaatcgtgt ggaggatatg gataaaaaac cttttggacg ggtggtcaat taccgctggc
    24421 cagctcataa tgagaaattg atgaatcagg ccttggccat ctatgcagat gatagtgctg
    24481 ctaatttatc agatgcggaa caaaaaaata acaatgaaaa aaatggactg gtggctaagc
    24541 ctttcaagga agtatttgct gaagtcaaac aaacggtcga ccaattgaga gagttgacgg
    24601 gtgatttcaa tgatattccg aagtctgaga aaaagcgtga ggctatgtta gaattgctcc
    24661 gtgcttacaa tcaaggactg tctaagctca aacagtatca gccagaggag gttgatgggg
    24721 tgattgaagg atttgattac aatcagccgg accgctttat tgaaagtttg ggaatgtctg
    24781 tagaggaaga aaaacttttg acgacggtct tagccaacga attgaaacga accttggcga
    24841 aagagcaaaa agttagcatt caccaaattg aactcaagat gactcacatc aaggaagtga
    24901 caattgacta cgataaattg accgaactgg ttgagctctt gctgaatcag gttcacgaag
    24961 agaagatggt agaagccgca gctaccaggg atgaattgga tagatttgca agcggtttgg
    25021 aagacctcaa ctatgctgat cagatccgtc aaacagggga tgcaatctat cgaggtgatt
    25081 ttccgacaca agatatgaac tttgcttatc cagtcaaggg gattgatagt cagaagatta
    25141 tccaagaagc ttcgcatgtt tacattgaca agaagctctt tgctttccga atccaatggg
    25201 gcttgacgga acatatccgc aaccaagaac tacgagagtt gttgacgaga catcgatatg
    25261 gtcagaatga cttggatgat accggacact tacgtacctt gataaaagaa gctagtcgcg
    25321 attaccagac ctgggcagaa ggtgatgctg ttaaaggctt gagcaagatt cagtaccgca
    25381 atcagttgag acaagcacta tatgatttgg ccgacgactt ggttggtcaa taggttgcga
    25441 ttttgttgga tgatagcgta taatagaatg gaaatcgtcc gaaaaattgc aaaaataact
    25501 ttcagaaata gaacaaaaag agagtagaaa aggagggact atgacaaaag aaaaatcaac
    25561 cgtaccacga ttgcgcttcc ccggattcac ggacgcttgg aaacagcgta agttggggga
    25621 ggtggcggac ttttccataa aaacaaactc actttctaga gataagttat catcgtattt
    25681 ttatgaagtt cagaacattc attatgggga tattttaaca aaatatgatg ctattttaga
    25741 tgtatgtaac aaagaacttc catcaattat tggaagtacg atttcagact ttgcagatgc
    25801 tttacttagt gaaggagata ttgttttcgc ggatgcggca gaagactcga ctgttgggaa
    25861 agctattgaa gttcgaaatt ttaagggtaa gaatgttgtt tctggtttac atacgatagt
    25921 tgctaggccg aaagtttctt atgcccccta ctatttaggt tatctaatta attcaactgc
    25981 ataccataat caaattttac ctttaatgca ggggacaaaa gtgagctcaa ttagtaaggc
    26041 taatttaaaa tccacgacag tagtgttccc caccctccct gaacaagaag ccatcggcag
    26101 cttcttctcc gacctagatc agcttattac tcttcatcag cgaaaattag atgatgttaa
    26161 agaattgaag aaggctttgt tacagaaaat gtttccgaaa gggaatggaa acgattttcc
    26221 tgagctaaga ttcccagaat ttacggacgc ttggaaacag cgtaagttgg gggagtttat
    26281 gaaagaaagt aaaattttag gtagtaaagg agatatagca aggaagttaa ctgtaagatt
    26341 atgggggaga ggagttgttt ccaagaaaga aatttatagt ggtagttcag ctacacagta
    26401 ttatataaga aaatcaggcc agtttatata tgggaaatta gactttttaa atcaagcttt
    26461 tggaattatt ccaccagaac ttgatggtta tgaatcaact cttgattcgc ctgcttttga
    26521 tttattgaaa gggataaacg ggcaatttct gttagaattt gtatctcgca aa
//
LOCUS       DBNFCK010000014        26522 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.014, whole genome shotgun sequence.
ACCESSION   DBNFCK010000014 DBNFCK010000000
VERSION     DBNFCK010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 26522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26522
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.014"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            203..1366
                     /locus_tag="AC1PIW_000462"
     CDS             203..1366
                     /locus_tag="AC1PIW_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HIP6605426.1"
                     /translation="MDVLYQSCAGIDVHQANIVVCILHGPLTSTRPKREMATFDTTTK
                     GLRACHDFLSQFHVEAVGMESTGVYWRPVWHALCDDFELILAQPAHMKAIPGQKTDKK
                     DAHWIAKLTRIGLLPRSFVPDETIQELRELTRQRKHYVESRNRETNRIHKFLQSGGIK
                     LTTYIEDIMGLSGRNLLQLLVDGTPITPRIVHQSVYTSLKKKVPQLLEALDGYFSDHH
                     RFMLKQSLEIYDFYQKQIELLEERITVYLSQYENHVETLDSIPGIDVITASVIISEVG
                     VDMSQFPTSGHLASWAGLCPGNNESAGKKRSTKIRHGNSYLKKCLCQAAFAARNQKGS
                     TLADRFYQIQSRRGSQKATIALAHQLLKIAYILLKEQMTYPEFLAQKRLLGTS"
     gene            complement(1904..2842)
                     /locus_tag="AC1PIW_000463"
     CDS             complement(1904..2842)
                     /locus_tag="AC1PIW_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HIP6605427.1"
                     /translation="MLDQIKAHLLDSINDIVSTANQFLLHPKKDFSRKSQLTRNLDER
                     AAFIDMLKTSSFKQALVIMDRGYESYNVMVHCQERNWSYIIRIRVGNHSMKSGFNLPD
                     TPCFDEAFDLNICRKQTNEMKQLYRDFPNHYRCLPNHTSFDFLPNSSRKRDPLQFYDL
                     HFRMVRLEIKPGFFETLVTNTDYSPEKLKDLYAYRWGIETSFRDLKYSIGLTHFHAKK
                     KEGILQEIYARFINFNVCKWLTSHVTIKTSKLKQTYKICFSDAVYACRKFLREELTSF
                     QLETYIAKHLSIIRPNRTFQRKIKSQAPVSFTYRIS"
     gene            2982..4202
                     /locus_tag="AC1PIW_000464"
     CDS             2982..4202
                     /locus_tag="AC1PIW_000464"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIP6605428.1"
                     /translation="MLENWLNTKQGQVFHYKMEKIEYALELLGNPQFAVPVIHVAGTN
                     GKGSTIAFMRKLFQAHGMRVGSFVSPHMVSVHDRICIDSQPISDHDFQHYLQKVYDLE
                     QEIATRYEPFRYFEVMVLIMFLYFEAQQPDVALVEVGIGGLLDTTNVVAPALSVITSI
                     GMDHQDLLGSTLREIAEQKAGIIKESVPVVLGPLSPETTAICRQIAQDNQAPVYQFGQ
                     EFTYKAGQFSNPDIDLSELVLGLAGHHQEENAAVALQTFLLYMTNIQKDIQPQLIQQA
                     LAQTSWPGRLELVVQEPKIYLDGAHNVPAIERLLEFIQVQEEPVTILFSALRRKDFQE
                     MLELLEEKLPHTPLVLTSFAYDGALAEENRQGRDYVENYQQFIEDWQSSKQGILIVTG
                     SLYFISEVRRIFKK"
     gene            4393..5916
                     /locus_tag="AC1PIW_000465"
     CDS             4393..5916
                     /locus_tag="AC1PIW_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol oxidase"
                     /protein_id="HIP6605429.1"
                     /translation="MTELDVVSDVVTHPEETYRRSRRASRRQQVQLNGEFHERVLKTK
                     YWPALVWSLVLSVFSVANPLLMPFATNIQTQNLYAGMAMANGQIPYGDFFGTSGLLFY
                     LLAFLGHLGGTFIIFGILQFIALLIAGVYFYKIVAYFSQSEHLAISSSHWFYVFIFAL
                     GFGGMYAEMFALPFLLTSVWFLVRYFENAVRDEAFILYGIDAALVFLIYPKSLILWLV
                     AGLVLFIFNIQHRQVTRGIYQLLATIFGFLLILYAVGYYAFEAQILGTAIQQTFLYNL
                     QLDFHHSYLYLALAIVSVFLLLSGFFKSFIQMVFSFKQGRHTYIKVLLLLTFLVQCVF
                     IIGNANFQWSQLILLLPYGFAMSVVYLRDEDVEDYSGYLRRQFFLPLAICLGIIAQPV
                     YLYLVQGDLRTDREQVANYIDEQTKDSDKIYVWDNSASIYLSSQRLSAATITTAEPYL
                     NTDDNKNSLMYDINKNEAKFVVVNKNLPILDEIKTNLESQYQSVQTTDYFTIYQKNE"
     gene            6034..7971
                     /gene="mutL"
                     /locus_tag="AC1PIW_000466"
     CDS             6034..7971
                     /gene="mutL"
                     /locus_tag="AC1PIW_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HIP6605430.1"
                     /translation="MSHIIELPEILANQIAAGEVIERPASVVKELVENSIDAGASQIE
                     ISVEEAGLKMIQITDNGEGIAPDEVALALRRHATSKIKNQSDLFRIRTLGFRGEALPS
                     IASVSHMIIETATADSAHGLHLEAKGGVIEKEEPVSRPVGTQITVSDLFYNTPARLKY
                     VRSQQAELSHIVDVVNRLSLAHPEIAFVLVNEGRELIRTAGTGKLRQAISGIYGIASA
                     KKMVEIEAEDLDFQISGYVSLPELTRANRNYISIFINGRYIKNFLLNRAILEGYGSKL
                     MVGRFPLAVISIEIDPYLADVNVHPTKQEVRISKEKELMTLIREAISQALKEQDLIPD
                     ALENLAQSSTRPKVKAEQGTLPLREPKIYYDTIKQDFFLKPDVVAEDVKPLEEDRQEI
                     VESPVENRPTSVQFAERQSVESEDQEHPNLSAKELAKLADKLDREETSTFPELEYFGQ
                     MHGTYLFAQGKTGLYIIDQHAAQERVKYEYYREKIGQVDNSAQQLLVPYIFEFPQNDA
                     LDLVHKMDALRQVGVNLEEYGSNQFILREHPIWMKEEEIESGIYEMCDMLLLTDQVSI
                     KQYRAELAIMMSCKRSIKANHALDDYSARDLLRQLSYCQNPYNCPHGRPVLVHFSKSD
                     MEKMFRRIQENHTSLRELGKY"
     gene            8010..8600
                     /gene="ruvA"
                     /locus_tag="AC1PIW_000467"
     CDS             8010..8600
                     /gene="ruvA"
                     /locus_tag="AC1PIW_000467"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIP6605431.1"
                     /translation="MYDYIKGILTKITAKYIVVETQGVGYILQVANPYAYSGQVQQEV
                     TVYTHQVIREDAHLLYGFATENEKSVFLSLISVSGIGPTTALAIIAVDDNDGLVRAIE
                     QKNITYLTKFPKIGKKTAQQMILDLEGKFVMSEEAGPVQQVAPSSENIALEEAMEAME
                     ALGYRPAELKKIKKFFEGTNDTAENYIKSALKMLMK"
     gene            9325..9894
                     /locus_tag="AC1PIW_000468"
     CDS             9325..9894
                     /locus_tag="AC1PIW_000468"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HIP6605432.1"
                     /translation="MSRCAWVNPNNPLYIAYHDEEWGKPLHDEQSLFELLCLESYQAG
                     LSWEIVLNKRQAFRSAFFNYDIQKVAAMTDSELDSLLTNPDIIRHKAKLYATRANAQA
                     FLRVQEEFGTFDTYLWEWVNFTSIDNPVKSFRELPTKNDLSERISKDLKKRGFKFVGP
                     VCIYSYLQAAGLLNEHEETCEIGKKLRTN"
     gene            9931..11112
                     /locus_tag="AC1PIW_000469"
     CDS             9931..11112
                     /locus_tag="AC1PIW_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HIP6605433.1"
                     /translation="MKAELIAVGTEILTGQIINTNAQFLSEKCAELGIDVYFHTAVGD
                     NEGRLLSTLEVASKRSNMVVLCGGLGPTEDDLTKQTLATFLGRNLVFDELAMAKLDRF
                     FASRPGRVRTPNNERQAQIVEGSQALQNPAGLAVGGMIEQDGVTYIVLPGPPSELKAM
                     FSESLLPLLSQSQQQLYSRVLRFFGIGESQLVTVLADVIDKQTDPTLAPYAKVGEVTL
                     RLSTKATSQEEANLRLNQLEEDILQHDKLADYFYAYGEDNSLVKTVATRLAEKRQTIA
                     IVEQGTGGLLQAELSLALADQPYFSGGKVVGQLGIESGWLSEEADCIRQELQADLGLA
                     VSVLIKPESTEDNVLAKVYLTLATPSGISQKEIDLGGYSWQYLRQLACLQAWDFVRNT
                     L"
     gene            11164..12315
                     /gene="recA"
                     /locus_tag="AC1PIW_000470"
     CDS             11164..12315
                     /gene="recA"
                     /locus_tag="AC1PIW_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIP6605434.1"
                     /translation="MAKKPGKKLEDITKKFGDERKKALDDALKSIEKDFGKGAVMRLG
                     ERAEQKVQVMSSGSLSIDIALGAGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKDGG
                     IAAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSV
                     AALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTVAIFINQLREKVGVMFG
                     NPETTPGGRALKFYASVRMDVRGNTQIKGTGDKKDQNVGKETKVKIVKNKVAPPFKEA
                     VVEIMYGEGISCTGELIEIGSNLGIIQKAGAWYSYNGEKIGQGSENAKKFLADNPAIF
                     DEIDRKIRVHYGLIEADGVEEVATEEAPVVAEEIQDVILDLDGGIELED"
     gene            12551..12949
                     /gene="spx"
                     /locus_tag="AC1PIW_000471"
     CDS             12551..12949
                     /gene="spx"
                     /locus_tag="AC1PIW_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HIP6605435.1"
                     /translation="MIKIYTVSSCTSCKKAKNWLNAHQLSYNEHNLAKEAITKEEILN
                     ILTKTENGIASIVSSKNRYAKSLDFDIEELSVNEVIDLITSNPRILKSPILIDEKRLQ
                     VGYKEDDIRAFLPRAVRNVENAQARMRAAL"
     gene            13049..13315
                     /locus_tag="AC1PIW_000472"
     CDS             13049..13315
                     /locus_tag="AC1PIW_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002885737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HIP6605436.1"
                     /translation="MGFTEETVRFRLDDTDKQEISKTLTSVYRSLEEKGYNPINQIIG
                     YVLSGDPAYIPRYNDARNQIRKHERDEIIEELVRYYLKGNGIDL"
     gene            13315..13734
                     /gene="ruvX"
                     /locus_tag="AC1PIW_000473"
     CDS             13315..13734
                     /gene="ruvX"
                     /locus_tag="AC1PIW_000473"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIP6605437.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIPIDEEKGEFGLER
                     LTELVEQYKVDKFVVGLPKNMNNTSGPRVEASQAYGDLLTERYKLPVDYQDERLTTVA
                     AERMLIEQADISRGKRKKVIDKLAAQLILQNYLDRTF"
     gene            13746..14066
                     /locus_tag="AC1PIW_000474"
     CDS             13746..14066
                     /locus_tag="AC1PIW_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HIP6605438.1"
                     /translation="MAHHHDHEHDHNHDERELITLVDDQGNETLFEILLTIDGQEEFG
                     KNYVLLIPASAEEDENGEVEIQAYSYIENENGTEGDLQPIPEDATAEWDMIEEVFNSF
                     MEEE"
     gene            14274..14852
                     /locus_tag="AC1PIW_000475"
     CDS             14274..14852
                     /locus_tag="AC1PIW_000475"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIP6605439.1"
                     /translation="MKDSFTERSKKLSEELERCLLADKNILVILDIMDRLNLSDCWLC
                     AGTIRNFIWNQYSFDEETDVDLVFFDENISYEETMEIESNLYQNYPAYQWELKNQVYM
                     HIHSPNTKPYQSSRDAIEKFPERCTAIGICRTERNKLEIFAPFGLDDIYAYQVRPTPH
                     FLADPNRMELYRQRILKKQWKKRWLSLTVKEI"
     gene            14925..15191
                     /locus_tag="AC1PIW_000476"
     CDS             14925..15191
                     /locus_tag="AC1PIW_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HIP6605440.1"
                     /translation="MEFETVCLKVRNLGRLYCYFKVKFRTHIEAIIRKQESQKCKFDR
                     LLHEDIHSLIQSPSLGNDELLMLWGLLRDYRKNLINDRLENMKN"
     gene            15515..15823
                     /gene="rpsJ"
                     /locus_tag="AC1PIW_000477"
     CDS             15515..15823
                     /gene="rpsJ"
                     /locus_tag="AC1PIW_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HIP6605441.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVETATRTGAQVAGPVPLPT
                     ERSFYTIIRATHKYKDSREQFEMRTHKRLIDIINPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            15920..16546
                     /gene="rplC"
                     /locus_tag="AC1PIW_000478"
     CDS             15920..16546
                     /gene="rplC"
                     /locus_tag="AC1PIW_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HIP6605442.1"
                     /translation="MTKGILGKKVGMTQIFTESGEFIPVTVIEATPNVVLQVKTVETD
                     GYAAVQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNIEGLEVGQEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGNRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            16571..17194
                     /gene="rplD"
                     /locus_tag="AC1PIW_000479"
     CDS             16571..17194
                     /gene="rplD"
                     /locus_tag="AC1PIW_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HIP6605443.1"
                     /translation="MANVTLFDQTGKQAGEVVLNDAIFGIEPNQAVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYAYKLP
                     QKVRRLALKSVYSEKVAENKFVAVNSLEFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNIPGVKVATATTASVLDIANADKLLVTQAAISKIEEVLA"
     gene            17194..17493
                     /locus_tag="AC1PIW_000480"
     CDS             17194..17493
                     /locus_tag="AC1PIW_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HIP6605444.1"
                     /translation="MNLYDVIKKPVITESSMGQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKTKRVGRYVGRTNKVKKAIITLAADSKAIELFATADAE"
     gene            17511..18344
                     /gene="rplB"
                     /locus_tag="AC1PIW_000481"
     CDS             17511..18344
                     /gene="rplB"
                     /locus_tag="AC1PIW_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIP6605445.1"
                     /translation="MGIKVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDGVEAIVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTVVHNIELKPGRGGELVRAAGA
                     SAQVLGQEGKYVLVRLQSGEVRMILGTCRATVGTVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNQ
                     K"
     gene            18595..18876
                     /gene="rpsS"
                     /locus_tag="AC1PIW_000482"
     CDS             18595..18876
                     /gene="rpsS"
                     /locus_tag="AC1PIW_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HIP6605446.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            18894..19238
                     /gene="rplV"
                     /locus_tag="AC1PIW_000483"
     CDS             18894..19238
                     /gene="rplV"
                     /locus_tag="AC1PIW_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HIP6605447.1"
                     /translation="MAEITSAKATARTVRVSPRKSRLVLDNIRGKSVADAIAILKFTP
                     NKAAGIIEGVLNSAIANAENNFGLEKANLVVSEAFANEGPTLKRFRPRAKGSASPINK
                     RTAHITVVVAEK"
     gene            19251..19904
                     /gene="rpsC"
                     /locus_tag="AC1PIW_000484"
     CDS             19251..19904
                     /gene="rpsC"
                     /locus_tag="AC1PIW_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HIP6605448.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRNFIKK
                     ELADASTSTIEIERAVNKVIVSIHTAKPGMVIGKAGSNVDALRAQLNKLTGKQVHINI
                     IEIKQPDLDAHLVGESIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEALTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            19908..20321
                     /gene="rplP"
                     /locus_tag="AC1PIW_000485"
     CDS             19908..20321
                     /gene="rplP"
                     /locus_tag="AC1PIW_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HIP6605449.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKQVDFGQYGLQATTSSWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEVAGVSEEIAREAFRLAGHKLPVKVKFVKREAE"
     gene            20331..20537
                     /gene="rpmC"
                     /locus_tag="AC1PIW_000486"
     CDS             20331..20537
                     /gene="rpmC"
                     /locus_tag="AC1PIW_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HIP6605450.1"
                     /translation="MKLQEIKDFVKELRGLSQEELAKKENELKKELFELRFQAAAGQL
                     EQTARLNEVKKQIARIKTVQSETK"
     gene            20561..20821
                     /gene="rpsQ"
                     /locus_tag="AC1PIW_000487"
     CDS             20561..20821
                     /gene="rpsQ"
                     /locus_tag="AC1PIW_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HIP6605451.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            20846..21214
                     /gene="rplN"
                     /locus_tag="AC1PIW_000488"
     CDS             20846..21214
                     /gene="rplN"
                     /locus_tag="AC1PIW_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIP6605452.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDIIVASVK
                     QATPGGAVKKGDVVKAVIVRTKTGARRADGSYIKFDENAAVIIREDKNPRGTRIFGPV
                     ARELRDGGFMKIVSLAPEVL"
     gene            21353..21658
                     /gene="rplX"
                     /locus_tag="AC1PIW_000489"
     CDS             21353..21658
                     /gene="rplX"
                     /locus_tag="AC1PIW_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIP6605453.1"
                     /translation="MFVKKGDKVRVIAGKDKGVEAVVVTALPKVNKVIVEGVNIVKKH
                     QKSNSENPQGAIVEKEAPIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            21682..22224
                     /gene="rplE"
                     /locus_tag="AC1PIW_000490"
     CDS             21682..22224
                     /gene="rplE"
                     /locus_tag="AC1PIW_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIP6605454.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGDA
                     VSNAKNLEKAAQELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVTVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGMDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            22239..22424
                     /locus_tag="AC1PIW_000491"
     CDS             22239..22424
                     /locus_tag="AC1PIW_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HIP6605455.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCEKCGRPHSVYRKFKLCRVCF
                     RDLAYLGQIPGVTKASW"
     gene            22761..23159
                     /gene="rpsH"
                     /locus_tag="AC1PIW_000492"
     CDS             22761..23159
                     /gene="rpsH"
                     /locus_tag="AC1PIW_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIP6605456.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIATILKNEG
                     FVKNVEFIEDDKQGIIRVFLKYGPNGEKVITNLKRVSKPGLRVYSKREDIPKVLNGLG
                     IAIISTSEGLLTDKQARQKNVGGEVIAYVW"
     gene            23625..24161
                     /gene="rplF"
                     /locus_tag="AC1PIW_000493"
     CDS             23625..24161
                     /gene="rplF"
                     /locus_tag="AC1PIW_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIP6605457.1"
                     /translation="MSRIGNKVITLPAGVELAQNNGVVTVKGPKGELTREFPTAIEIR
                     VEGAEVTLHRPNDSKEMKTIHGTSRANLNNMVVGVSEGFKKELEMRGVGYRAQLAGNK
                     LTLAVGKSHPDEVVAPEGITFEVPTPTQIVVSGINKEVVGQTAAYIRSLRAPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            24250..24606
                     /gene="rplR"
                     /locus_tag="AC1PIW_000494"
     CDS             24250..24606
                     /gene="rplR"
                     /locus_tag="AC1PIW_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HIP6605458.1"
                     /translation="MISKPDKNKIRQKRHRRVRGKISGTAARPRLNIFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVVVGKLVAERAVAKGISEVVFDRGGYLYHG
                     RVKALAESARENGLKF"
     gene            24625..25119
                     /gene="rpsE"
                     /locus_tag="AC1PIW_000495"
     CDS             24625..25119
                     /gene="rpsE"
                     /locus_tag="AC1PIW_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HIP6605459.1"
                     /translation="MAFKDNAVEIEERVVAINRVTKVVKGGRRLRFAALVVVGDRNGR
                     VGFGTGKAQEVPEAIRKAVESAKKNMIEVPMVGTTIPHEVRSEFGGARVLLKPASEGS
                     GVAAGGATRAVIELAGIADVTSKSLGSNTPINIVRATVEGLKQLKRAEEVAALRGISV
                     SDLA"
     gene            25134..25316
                     /gene="rpmD"
                     /locus_tag="AC1PIW_000496"
     CDS             25134..25316
                     /gene="rpmD"
                     /locus_tag="AC1PIW_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HIP6605460.1"
                     /translation="MAQIKITLTKSPIGRKPEQRKTVVALGLGKLNSSVVKEDNPAIL
                     GMVNAISHLVTVEEVK"
     gene            25452..25892
                     /gene="rplO"
                     /locus_tag="AC1PIW_000497"
     CDS             25452..25892
                     /gene="rplO"
                     /locus_tag="AC1PIW_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HIP6605461.1"
                     /translation="MKLHELQPATGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRPGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNAFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITSKGGSVEVI"
     gene            25906..>26522
                     /locus_tag="AC1PIW_000498"
     CDS             25906..>26522
                     /locus_tag="AC1PIW_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0065002 - intracellular protein
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIP6605462.1"
                     /translation="MFFKLLKDALKVKLVRSKILFTIFILFVFRVGTHITVPGVNAKS
                     LEALSNLPFLNMLSLVSGNAMRNFSVFALGVSPYITASIIVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYISLVLAFVQSIGITAGFNALSGAKLTNMPLNWQTYLLIGAILTT
                     GSIIVTWLGEQISEKGYGNGTSMIIFAGIISSLPGTFHEIYIDRFV"
BASE COUNT         8228 a         5052 c         5867 g         7375 t
ORIGIN      
        1 atgaaaacga acataaagcc ctcactaggt gtataatctt agtgagggct ttgtggttaa
       61 cgagttaatg atgttagtca tcgcgggaaa aactaactca ctctactgac gagcacccca
      121 aaatatgcga caggcatgag tataaaaata cgactaaaat tcggtagata agctctctaa
      181 tttttagaag gagggaatta tcatggatgt tctctatcaa tcttgtgcag gtattgatgt
      241 ccatcaagcc aatattgttg tctgtatcct acatggacca ctcacctcaa ctcgtccaaa
      301 gcgtgagatg gctacatttg atacaacgac taaaggccta cgtgcttgcc acgattttct
      361 cagtcaattt catgtggaag ctgttggtat ggaaagcacc ggtgtctatt ggcgacctgt
      421 ctggcatgct ctctgtgatg acttcgagct gatactcgct caaccagccc acatgaaggc
      481 tattccaggt cagaaaaccg acaagaagga cgctcactgg attgccaaat taacacggat
      541 tggtctgctt cctcggagtt tcgttcccga tgaaaccatt caagaattga gggagttgac
      601 cagacaacga aaacattatg tggaaagtcg caatcgagaa acaaaccgta tccataaatt
      661 tcttcagtca ggtggtatca agctaacaac ctatattgaa gatattatgg ggctatctgg
      721 tcgcaatctc cttcagctac tggttgatgg gacgcctatt acacctcgca ttgttcatca
      781 atccgtttat accagcttga agaagaaagt gccgcagctt ctggaagcct tggacggtta
      841 tttctctgac catcatcgct ttatgttaaa gcagtcctta gagatttatg atttttatca
      901 gaagcagatt gagttgttgg aagagcgaat tactgtctat ctatcacaat atgaaaacca
      961 tgtagaaaca ttggattcca tcccaggcat tgatgtcatt acagcttctg ttatcatttc
     1021 tgaggttggt gttgacatga gtcagtttcc gacatctgga catttagctt cttgggctgg
     1081 actctgtcca ggtaataatg agagtgctgg taaaaaacga agtaccaaga ttcgacacgg
     1141 aaattcatat ttgaagaaat gtttatgtca agccgctttc gccgctagga accagaaagg
     1201 aagtacccta gcagaccgat tttatcagat tcaaagtcgg cgtggctcac aaaaagcaac
     1261 cattgcactt gcgcatcaac tattaaaaat agcctatatc cttttaaaag aacagatgac
     1321 ttatcctgaa tttttagcac aaaaaagact actagggacg agctagtagc ctaatataaa
     1381 aattgtttca ctttgattct atcataggga gggagtttct gcattctttt gagttttcgt
     1441 ataagaactc gactggtcaa aaagagttcg tcaacaagtc tttatttcta gttgttgagc
     1501 tgaaacagtc tattcccaga catcaattta tgtgagctga ctgccgtcag cttatatctc
     1561 ccactttaaa aggtctccca gaccttttaa actcccaccc ccgtatagct ccaacagtca
     1621 gagtagtgac tgttggaggt tggagataag gcgaactctg ttcgcatcag tcgtaatggt
     1681 cagatttgga gtgtaaaaca cgaacaaatc taccaatcaa ccactgcgct gagatgttga
     1741 cacgaattct gagaagtgtg ctggcttgag cccaatgtca ttaagttaag catttaaaca
     1801 attaaagaaa ctattcccgt ctgagcagtt gatgacaaga cgggaatagt ttttgtattt
     1861 agggcacaca aaaaggaagg tttttaaccc aattttttaa ctattatgat attctataag
     1921 tgaagcttac gggagcctgg ctttttatct ttctttggaa ggttcgattg ggtcggatga
     1981 tggataaatg tttggcaatg taggtttcta attggaagga agtaagttct tctctaagaa
     2041 attttcgaca ggcataaaca gcgtctgaaa aacaaatttt ataagtctgt tttaactttg
     2101 atgttttaat ggtaacgtgt gaggttagcc atttacaaac attaaagttg ataaagcgag
     2161 cgtagatttc ttgcagaatc ccttccttct ttttcgcatg aaaatgagtc aacccaatac
     2221 tgtattttag gtcacgaaaa ctggtctcta tgccccatct gtaggcatag agatctttta
     2281 atttttctgg tgaataatcc gtatttgtca ccaaagtctc aaagaaacct ggcttgattt
     2341 cgagacgcac catccgaaaa tgaaggtcat aaaactggag tggatccctt tttcggctag
     2401 agtttggtaa aaagtcaaag gatgtgtgat taggtaaaca gcgatagtga ttaggaaagt
     2461 ctcgatacag ttgtttcatc tcattggttt gttttcgaca gatgtttagg tcaaatgcct
     2521 catcaaaaca aggggtgtca gggaggttaa atcctgactt catagaatga ttcccgacac
     2581 gaatacgaat aatataggac caatttcttt cttgacagtg taccatgaca ttgtaggatt
     2641 cataccccct atccattatc accagagctt gtttgaaaga agaggtcttc agcatgtcaa
     2701 taaaagctgc tcgttcatca agattcctag ttagctgact tttccgacta aaatcttttt
     2761 taggatgaag taaaaactgg tttgcggtgg aaacgatgtc gttaatacta tcaagtaaat
     2821 gagctttaat ctgatctagc atgacttttc cccttttatt tttagtgtac tatagaaaaa
     2881 actaacctac cacaaaacgt gataggttag ttaacttaat gacattgggc tttttgccca
     2941 gcctcttttt tgttatacta gacagtatgt aggaggtggc tatgttagaa aattggttaa
     3001 acaccaaaca aggtcaggtg tttcattaca agatggaaaa gattgagtat gccctagaac
     3061 tgctagggaa tccccagttt gcagttccgg tcattcatgt cgctggaact aatggcaagg
     3121 gatcgaccat tgcctttatg cgcaagctat ttcaggcaca tggtatgcgt gttggaagtt
     3181 ttgtatcccc ccacatggtg agtgtacacg acaggatttg tattgacagc cagcccattt
     3241 cagaccatga ttttcagcac tatttacaga aagtctacga tttggagcag gaaatcgcca
     3301 ctcgttatga gccttttcgc tattttgagg tcatggtgct cattatgttc ctctatttcg
     3361 aagctcaaca acccgatgtg gcactagtag aggtgggcat cggaggactt ttggatacga
     3421 ccaatgtcgt ggccccagcc ctaagcgtta tcacctccat cggcatggac catcaggatt
     3481 tattaggctc gactttaagg gaaatagcag agcagaaagc agggattatc aaggaaagcg
     3541 tacctgtcgt cctaggaccg ctttctccag aaaccacagc catctgtcgc caaattgccc
     3601 aagacaatca agcacctgtc taccaatttg gtcaggaatt cacctataaa gcaggacagt
     3661 tcagcaatcc ggacatcgac ctgtcagaat tggttttggg tctggctggc catcaccaag
     3721 aagagaatgc ggccgtagcc ctacaaactt ttctactcta tatgaccaat attcaaaaag
     3781 acattcaacc tcagttgatt caacaagccc ttgcccaaac cagctggcct ggtcgtttgg
     3841 aattggtagt tcaagagcca aaaatctatt tggatggagc ccacaatgtc cccgctatcg
     3901 aacgtttgct tgaatttatt caggttcaag aagagcctgt caccattctc ttttcggccc
     3961 ttcggcgcaa ggactttcaa gaaatgcttg aattattaga agaaaaattg ccacatactc
     4021 cacttgtatt aaccagcttt gcctatgatg gtgccttggc tgaggaaaac cgacaaggac
     4081 gagattatgt tgagaattat cagcaattta tagaagactg gcaatctagc aagcaaggga
     4141 ttttgattgt tacaggctct ctctacttta tctcagaagt tcgtcggatt tttaaaaaat
     4201 gaatatcttt ttgcgaataa ataggtatga cacatgcaga aaaagtagcc tttttaaaag
     4261 aatttcaaga gaacaaacgc catacttttc gccaattaga acagtttgca aaaatttcca
     4321 gaactcccga taggaaatga catcgggagt gctttttgat ataataagga aagcaaatcc
     4381 aaggagaaga aaatgacaga attagatgta gtttcggacg tagttactca tcctgaggaa
     4441 acttatcggc gtagtcgtag agcatcacgt agacagcaag tgcaactgaa tggtgaattt
     4501 catgaaagag tattaaagac aaagtattgg ccagctcttg tttggagctt agttttaagt
     4561 gtttttagcg tagccaatcc actgctaatg ccatttgcaa ccaatataca aactcaaaat
     4621 ttatatgcag ggatggcaat ggcaaatgga caaatccctt atggtgattt ctttggtaca
     4681 agcgggttat tattctactt acttgctttc ttaggtcatc tgggaggaac tttcattatc
     4741 tttggaatcc tccagtttat agcattactg atagcgggag tctatttcta taagattgta
     4801 gcttatttta gtcagtcaga acacctagcg ataagttcta gtcattggtt ctatgtattt
     4861 atttttgcac ttggttttgg agggatgtat gcagaaatgt ttgctcttcc gtttcttttg
     4921 actagtgtgt ggtttctggt ccgctatttt gaaaatgcag tgcgtgatga ggcatttatc
     4981 ctctacggaa ttgatgcggc attggtattt ttaatttacc caaaaagtct gatcctttgg
     5041 ttagttgcgg gtctagtttt atttattttt aatatccaac accgccaagt tactcgagga
     5101 atctatcaat tattagcgac catttttggt tttcttctga ttctatatgc ggtaggttat
     5161 tatgcttttg aggcgcagat tctggggaca gctattcaac aaactttcct ttataactta
     5221 cagttagact ttcaccattc ctatctatat ttagccttgg cgattgtgtc tgtattttta
     5281 ctcttatctg gattctttaa aagtttcatc caaatggtgt tttctttcaa acaaggaaga
     5341 catacctata tcaaagttct cctattattg actttcttag ttcaatgtgt ctttattatt
     5401 gggaatgcaa actttcaatg gagccaactc attcttcttt taccgtatgg ttttgcgatg
     5461 tcagtcgttt acctgcgtga tgaggatgta gaagattata gtgggtattt gcgtcgtcaa
     5521 ttctttcttc cgttagctat ttgcttgggt attatcgctc aacctgttta tctttatttg
     5581 gtacagggag atttgagaac tgaccgtgaa caagttgcca attacattga tgaacaaaca
     5641 aaagattcgg ataagattta tgtttgggat aatagcgcaa gcatctatct ttcaagtcaa
     5701 cgtttatctg ctgcaacgat tactacagca gaaccctatc tgaatactga cgacaataaa
     5761 aatagcttga tgtatgacat caataaaaat gaggcaaaat ttgtagtcgt caataagaat
     5821 ttgccaattc tagatgaaat caagacaaat ttagaatcac agtaccagtc tgttcagaca
     5881 acggattact ttacgattta tcaaaagaat gaatgattgc tcaggcagtc attttttctt
     5941 tagcctatct gggaaaagga gggaaagtgg atatgtggta taatggaaaa ataagcattc
     6001 gggaaaaatt attaaagaaa tgtaggagaa atgatgtcac atattattga actaccagaa
     6061 atactagcca accagattgc ggctggtgag gtcattgagc gaccagccag tgtggttaag
     6121 gagttggtgg aaaattccat tgacgcaggg gctagtcaga ttgaaattag tgttgaagag
     6181 gctggcctca aaatgattca aattacggat aacggtgagg ggattgcccc tgatgaagta
     6241 gcgcttgccc tccgccgtca cgccaccagt aagataaaaa atcaatcgga tttgtttcgt
     6301 attcgcaccc tcggttttcg tggagaagcc ctgccctcca ttgcttctgt cagtcatatg
     6361 atcattgaga cagctacggc agactctgca cacggacttc atttggaggc caaaggtggt
     6421 gtcatcgaga aagaggagcc agttagtcgt ccagttggta cgcagattac tgtttcggat
     6481 ttattttaca atacccctgc tcgtctcaaa tatgttcgca gtcaacaggc tgaattatca
     6541 catattgttg atgttgtaaa tcgattgagt ctagctcatc cggaaatagc ctttgtctta
     6601 gtaaacgaag gacgagaatt aattagaact gcagggacag ggaaacttcg tcaggcaatt
     6661 tcagggattt atgggattgc ttctgccaaa aaaatggttg aaattgaggc agaagatctg
     6721 gattttcaga tttcaggtta tgtttccttg ccagagttga ctcgtgccaa ccgcaactac
     6781 atttccattt ttatcaacgg tcgctatatc aagaattttt tgcttaatcg agcgattttg
     6841 gaaggttacg gcagtaagtt gatggttgga cgctttccgc tggcagtcat ttctatagaa
     6901 attgacccct atcttgctga tgtcaatgtg catccgacca agcaggaagt tcgcatctcc
     6961 aaggaaaaag aactcatgac cttgattcga gaggcgatta gtcaagcact taaagagcag
     7021 gacttgatac cagatgctct tgagaattta gctcagtcaa gtactcgacc aaaagtaaaa
     7081 gcagagcaag gcaccttacc actcagagag ccaaaaatct attatgatac gattaagcaa
     7141 gacttcttct taaaaccaga cgtggtcgct gaggatgtca aacctctcga agaggatagg
     7201 caagagattg ttgagtcgcc tgtcgaaaat agacctacat ctgttcaatt tgcagaacgt
     7261 cagtcggtgg aatcagaaga tcaggagcat cctaatctaa gtgcaaaaga attggcaaaa
     7321 ctggcagata aattagaccg ggaggaaaca tcgacatttc cagagttaga atattttggt
     7381 caaatgcatg ggacgtattt atttgcacaa ggcaagacag gactttatat cattgaccag
     7441 catgccgcac aagagagggt caaatacgaa tactaccgtg agaaaattgg acaagttgac
     7501 aattcagctc aacagttatt ggtgccgtat atttttgaat ttccgcagaa tgatgccctt
     7561 gacctcgtcc acaaaatgga tgcccttcgt caagttggtg tcaacttgga agaatatgga
     7621 tctaatcaat ttattctgcg tgaacatcct atttggatga aggaagagga gattgagtct
     7681 ggcatttatg agatgtgcga catgttgctc ttgacggatc aggtgtcaat caagcagtat
     7741 cgggcagaac tggccatcat gatgtcttgt aaacggtcaa tcaaggccaa tcatgctttg
     7801 gatgattatt cggcgcgtga tttattgaga caattgtctt actgccaaaa tccctataat
     7861 tgcccgcacg gccgcccagt cttagtgcat tttagcaaat cggatatgga aaaaatgttc
     7921 cgtcgcattc aagaaaatca cacgagtttg cgtgaattag ggaagtatta acatagttga
     7981 aaaagttgac actttagcaa aggaagtata tgtacgacta tattaaagga attttaacaa
     8041 aaataactgc aaaatacatt gtggtagaaa cgcaaggagt aggctatatc ttgcaggttg
     8101 ctaatcccta cgcctattca ggacaagtcc agcaagaagt gactgtctat actcatcagg
     8161 tgattcgtga agatgctcat ttgctctacg gatttgctac agaaaatgaa aaatccgtct
     8221 ttctgagtct gatttcagta tcaggtattg gtccaacaac agctctggct attattgctg
     8281 ttgatgataa tgatggactt gttcgtgcta tcgagcagaa aaacattacc tacctgacca
     8341 aatttccaaa gattggcaag aaaacagccc agcagatgat tttggacttg gaaggcaagt
     8401 ttgtcatgag cgaagaagcg ggtcctgttc aacaagtagc accatccagt gaaaatatcg
     8461 cccttgaaga agccatggaa gccatggaag cccttggtta ccgcccagcc gaactcaaga
     8521 aaatcaagaa attctttgaa ggcaccaacg acaccgcaga aaactacatc aagtcagccc
     8581 ttaaaatgct gatgaagtaa taagatacaa aggacgtaaa ggacaaagag aaaatagacg
     8641 gccgaacaga aattttgcta gaaatttagt tgtgagacct ccgcaatgac aactaggtaa
     8701 aaggcgagtt ttgacaaagc cttttgccag atgtcaactg cgttattcgg tgatatttag
     8761 gattcagttt gacaattttt caaagtatcg tagccgtgtt caactaacgt accagtatga
     8821 tggttaaaaa gttttgtttt gttactcaaa gtttgagtca acatttcagc gcagtggttg
     8881 attggcagat ttgttcgtgt tccacacatc aaatctggcc taatcaactg tgcgggggca
     8941 ggaagacgaa ttctttttct gcctgtcgaa ttctttcccg ctcccactac agcaatcgct
     9001 atttgcgact tgcctaggta ccttactaac tcgccacaaa aataatgtcg atttttgtgg
     9061 ctcatgtcgt gtccagatac aaagggcgta aaggacaaag agaaaataga cggccgaaca
     9121 gaaattttgc aagaaatttc gttgtgagac ctccgcaatg acaactaggt aaaaggcgag
     9181 ttttgacgaa gccttttgcc agatgtcaac tgcgttactc agtgatatgt aggattcagt
     9241 ttgacaattt tgcaaagtat cgtagccgtg tttcagtctg tttatcctga ttcgtacttt
     9301 agttattcac gaaaaggagt ttctatgtcc cgttgtgctt gggtcaatcc caacaatcca
     9361 ctttatattg cttaccatga tgaagaatgg ggcaagccac ttcacgatga gcagtctttg
     9421 tttgagttgc tttgcttgga atcctaccaa gcaggacttt cctgggaaat tgttctcaat
     9481 aaacgccagg cttttcgctc tgcttttttc aactatgata ttcaaaaggt tgcggccatg
     9541 acagatagcg agttagatag ccttctgacc aatccagaca tcattcgtca taaggcaaag
     9601 ctgtacgcta cccgcgccaa tgctcaggcc tttcttcgag tacaggagga attcggtacg
     9661 tttgatacgt atctttggga atgggttaac tttacatcta tcgacaaccc tgtcaagtcc
     9721 ttccgagaat tgccgaccaa gaacgacttg tctgagcgga tttcaaaaga tttgaagaaa
     9781 cgaggtttca aatttgtagg gcctgtctgt atttactcct atttgcaggc tgcaggtctg
     9841 ttgaatgaac acgaagaaac ttgcgagatt ggaaagaaat tacgaactaa ttagatgagc
     9901 gagcaatcgc accctagaca gaggaggaat atgaaagcag aactaatcgc cgttgggacg
     9961 gagattttga caggtcaaat cattaatacc aatgctcagt tcctttcgga gaaatgtgca
    10021 gagctgggaa ttgatgttta ttttcatacg gcagttggtg acaatgaagg ccgtttatta
    10081 tcaacccttg aagtagccag caagcggagt aatatggtcg ttctttgcgg tggtttagga
    10141 ccaactgagg acgacctgac caagcagacc ttggcgacct tcttaggtag aaatcttgtg
    10201 tttgatgagt tggctatggc aaaattagac cgtttctttg ccagtcgccc aggtcgtgtc
    10261 cgtacaccaa ataatgaacg tcaggcacag attgtggagg gaagtcaggc gctacagaat
    10321 ccagctggtt tagctgttgg tggtatgatt gagcaagatg gtgtaaccta tattgttttg
    10381 cctggcccac caagtgagct caaggccatg ttttctgaga gtctcttacc tttactgtcc
    10441 caatctcagc agcaacttta ttcgcgtgtc ctacgctttt ttggaattgg cgaaagccag
    10501 ttggtgactg ttttggcgga cgtgattgat aagcagacag acccaactct tgctccttat
    10561 gcaaaagttg gagaggtgac tttacgcttg tccaccaaag cgaccagcca agaagaagca
    10621 aatctacgtt taaatcagtt ggaagaagac atcttacaac atgacaaact ggcagactat
    10681 ttctatgcct acggagagga caatagtttg gtcaaaacgg tagcgactcg tttggcggag
    10741 aaaagacaaa ccatcgctat cgtcgaacag gggacaggtg gtctcttgca agctgaattg
    10801 agcctggctt tggctgatca gccgtatttt agcggaggaa aagtcgtcgg tcagctaggg
    10861 atagaatcgg gctggctatc agaggaagct gactgcattc ggcaggagct gcaagctgat
    10921 ttaggtttgg ctgtgtctgt gcttatcaaa ccggaatcaa cagaggacaa cgtacttgca
    10981 aaagtatacc tcactttggc tacgccctcg ggtatttccc aaaaagagat agatttaggt
    11041 ggttattcgt ggcaatacct tcgccagctt gcttgtctgc aggcctggga ttttgtacga
    11101 aacactttgt gaaatagcag atatttgcta taatagtttg actaagaaaa gaggagaaaa
    11161 tcattggcta agaaaccagg aaaaaaatta gaagatatta caaagaaatt tggcgatgag
    11221 cgtaaaaaag cgttggatga tgccctaaaa tcaattgaaa aagattttgg taagggtgct
    11281 gttatgcgtc ttggtgagcg tgctgagcaa aaagttcaag tcatgagctc aggtagctta
    11341 tcaatcgaca ttgcgcttgg agcaggtggc tatcctaagg gccgtatcat tgagatttac
    11401 ggtccagaga gctcaggtaa gacaactgtt gctctacacg cagtagctca agcccagaaa
    11461 gatggtggta ttgctgcctt tatcgatgcg gagcatgcct tggatccagc ctatgcggcg
    11521 gctcttggtg tcaatattga tgagttacta ttgtcacagc cagactcagg ggaacaaggt
    11581 cttgaaattg caggcaagct gatcgactct ggtgcggttg acttggttgt tgttgactcg
    11641 gttgcagccc ttgtaccacg tgcagaaatt gacggtgata tcggtgatag ccacgttggt
    11701 ttgcaggctc gtatgatgag ccaggctatg cgcaaactcg gtgcatccat caacaaaact
    11761 aagacagtag ctattttcat caaccaatta cgtgaaaaag tcggtgtcat gtttggtaac
    11821 cctgaaacaa cgcctggtgg acgtgcgctt aaattctatg catctgtccg tatggatgtt
    11881 cgtggaaaca cacaaatcaa aggaacaggt gacaagaaag atcaaaacgt tggtaaggaa
    11941 accaaggtga agattgtgaa gaacaaggtg gctccaccat ttaaagaagc tgttgttgaa
    12001 atcatgtacg gtgaaggaat ttcttgcaca ggtgaattga ttgagattgg tagcaacctc
    12061 ggcatcatcc aaaaagcagg ggcttggtat tcatataatg gagaaaaaat tggccaaggt
    12121 tctgaaaatg ctaagaaatt cttagcagat aatccagcaa tctttgatga aattgaccgc
    12181 aagatacgtg ttcattatgg cttgattgaa gcagatggag ttgaagaagt tgcgaccgaa
    12241 gaagctcctg ttgttgcgga agaaatccaa gatgttatcc tagatcttga tggcggtatt
    12301 gaattagaag attaattcct aacaaaatag atttaatatt gaaccattga tgcatatctg
    12361 ttataggtgt gtatgaagtt gtttagtata agaacgaaaa agtctggaga ttccaggctt
    12421 ttttctaata aaaaccgaat ttttacccca aaatttaaag tttttttaaa aaataggtct
    12481 gcagaccgag ggaatttatg atataatagt ctataatgtg cttaataaaa tgtagcaaag
    12541 gagtgactac atgattaaaa tttatactgt atctagttgt acttcttgta agaaagcgaa
    12601 aaattggtta aatgctcacc aattatctta taatgagcat aatcttgcga aggaagccat
    12661 tactaaggaa gaaatcctaa acatcttaac aaaaacagag aatggtattg caagtatcgt
    12721 atcgtcaaaa aaccgttatg caaagagtct ggacttcgat attgaagagt tgagtgtgaa
    12781 cgaggtcatt gatttaatta cctctaaccc acgtattttg aagagtccaa tcttgattga
    12841 tgagaaacga cttcaagtgg gctataaaga agatgatata cgcgctttct tgccacgtgc
    12901 agtgcgcaat gttgaaaatg ctcaagctcg tatgagagca gctctataag atgtacaaaa
    12961 ggactctttt ggggttcttt ttgcgcattt tttgagaata attaaaattt gtgatataat
    13021 gtaaaaaata gggaaagaag gtgtaagtat gggatttacc gaagaaaccg ttcgttttcg
    13081 cctagatgat acagataaac aagaaatcag caagacattg actagtgttt atcgttcgct
    13141 ggaggaaaag ggctataatc cgattaacca aatcattggc tatgtattaa gtggggaccc
    13201 tgcttatatt cctcgctata atgatgcccg caatcagatt cgtaagcatg aacgagatga
    13261 aatcattgaa gaattggtgc gctactattt gaaagggaat gggattgacc tctaatgaga
    13321 ataatgggat tagacgtcgg ttccaagaca gttggtgtag ccatttcaga tccgttaggt
    13381 ttcacggccc aagggttgga aatcatccca atcgatgaag aaaagggcga attcggtctg
    13441 gagcgtttga ccgaacttgt agaacagtac aaggttgata aatttgttgt aggcttgccg
    13501 aagaatatga ataatactag tggtccacgt gtggaagcta gtcaagctta cggtgattta
    13561 ttgactgagc gttacaaact tccagttgat tatcaggatg aacgtttaac aactgttgca
    13621 gcagaacgga tgctgattga gcaagcggat atcagccgag gaaaacgcaa gaaagttatc
    13681 gataaattag cagctcagct gattttacaa aattatttag atagaacatt ttaaaggaga
    13741 ttctaatggc acatcatcat gaccatgaac acgaccacaa ccacgacgaa cgtgaattga
    13801 ttactttggt agacgaccaa ggtaacgaaa cactttttga aatcttattg acgattgacg
    13861 gtcaagaaga gtttggtaag aactatgttc tcttgattcc tgcaagtgca gaagaagatg
    13921 agaacggaga agttgaaatc caagcctact catacattga gaatgagaat ggcacagaag
    13981 gcgatttgca acctatccca gaagacgcaa cagcagaatg ggatatgatt gaagaagtct
    14041 tcaacagctt tatggaagag gaataaaaag gtccgggtgg acctttttat catgagcatg
    14101 aaaacaagaa agcgaattaa ggtccagtgg acctttttat catgagctga aaatcaaaga
    14161 gcgaattaag gtccggtgga gtgaagcagt ctgggggaga ctgtttcagc ccgagttaag
    14221 aaatagcaag gggaggaatg aatcatgagc cagaaaatca aaagggaggc cagatgaaag
    14281 atagttttac agaaagaagt aagaagctga gtgaggaatt agagaggtgt ctactggctg
    14341 ataaaaatat tttggtcata ttagacataa tggatagact gaatttatca gattgctggc
    14401 tctgtgcggg aactatccgt aatttcattt ggaatcaata cagttttgat gaagaaacag
    14461 atgttgatct tgtgtttttt gatgaaaata tttcttatga agagacaatg gaaatagaaa
    14521 gcaatttata tcaaaactat ccagcctatc aatgggaatt aaaaaatcaa gtgtatatgc
    14581 atatccatag tccgaatact aaaccgtatc aatcatcaag ggatgcgatt gaaaaatttc
    14641 cagaaagatg cacagcgata ggtatttgtc gaacagaaag aaataaatta gaaatatttg
    14701 ctccttttgg attggatgac atttatgctt accaagtacg gccaacacca catttcttag
    14761 cggatcctaa tcgcatggaa ttgtatcgac aaagaattct taagaaacag tggaaaaaga
    14821 ggtggctatc gctaacagta aaagagattt aactcccacc aagtaagtag gccaaaaaca
    14881 gtatttttta taaactaaaa caatcaacta cgggagatta tgttatggaa tttgaaacgg
    14941 tttgtttgaa ggttagaaac ttaggtaggc tatattgcta cttcaaagtt aaatttagaa
    15001 cccatatcga ggctataatt cgtaaacagg aaagtcaaaa atgtaaattt gatcgtctcc
    15061 tccatgaaga tattcatagt ttgattcaat caccgagctt gggtaatgat gagttgctga
    15121 tgttatgggg tctcctgaga gattatcgaa aaaatttaat caatgaccga cttgaaaata
    15181 tgaaaaattg ataagcggac agagttttac tggcagtcaa aaaaataatt cgagacttac
    15241 aaggatattt gaaaaaaaaa cgttgatagt taactcaaat aaaaaataag taatgaagaa
    15301 tcctgaaata aaagttttgg gattttttga atttttgagt aaaaactctt gcaatggcaa
    15361 gtggaatctg gtagaatgtt ctagtgccgt aaaaattacg gctgtagctt tgatgcaaga
    15421 ggttgcgaca cgctcggttg cattgccacg caacacgtgt tggttttctt gtggagctag
    15481 cctattatgt taaatagacg aaaaggagaa aaagatggca aacaaaaaaa ttcgcatccg
    15541 cttgaaagcg tacgaacacc gtacacttga tacagcagct gcaaaaatcg ttgaaactgc
    15601 aacacgtaca ggtgctcaag tagcaggtcc agttccactt ccaacagaac gtagcttcta
    15661 cacaatcatc cgtgcgactc acaagtacaa agattctcgt gagcagttcg aaatgcgtac
    15721 acacaaacgt ttgatcgaca tcatcaaccc aactcaaaaa acagttgacg ctttgatgaa
    15781 attggatctt ccaagtggtg taaacgtaga aattaaattg taatcaagtc gcgagcaaaa
    15841 ctcagaatct tgatttgagc atgaaaaacg ctcgttaaaa actttttaaa tacaaaataa
    15901 gaaaaggaat atttttctca tgacaaaagg aatcttaggg aaaaaagtgg gaatgactca
    15961 aatcttcact gaatctggtg aatttatccc tgttactgtc atcgaagcaa ctccaaacgt
    16021 tgttcttcaa gtgaaaacag ttgaaactga tggttatgca gcagttcaag ttggttttga
    16081 tgacaaacgt gaagtattga gcaacaaacc tgccaaaggc catgtagcta aagctaacac
    16141 agctcctaag cgcttcattc gtgaattcaa aaacattgaa ggcttggaag ttggacaaga
    16201 aattacagtt gaaactttcg cagctggtga tgttgttgat gtaactggta catctaaagg
    16261 taaaggtttc caaggtgtta tcaaacgcca tggtcaatca cgtggtccta tggctcacgg
    16321 ttctcgttac caccgtcgtc caggttctat gggtcctgtt gcacctaacc gtgtattcaa
    16381 aggtaaaaac cttgcaggtc gtatgggcgg caaccgtgta acaattcaaa accttgaagt
    16441 tgtacaagtt gttccagaaa agaacgttat ccttatcaaa ggtaacgtac caggtgctaa
    16501 gaaatctctt atcactatca agtcagcagt caaagctggt aaataataag gaaaggggaa
    16561 atcagtcgaa atggcaaacg taacattatt tgaccaaact ggtaaacaag ctggtgaagt
    16621 agttcttaac gatgcgatct ttggtatcga gccaaaccaa gcagttgtat ttgatgtgat
    16681 catcagccaa cgtgctagcc ttcgtcaagg tactcacgca gttaaaaacc gttcagcagt
    16741 ctcaggtggc ggacgcaaac catggcgtca aaaaggaact ggacgtgctc gtcaaggttc
    16801 tatccgttca ccacaatggc gtggcggtgg cgtagtcttc ggaccaactc cacgttcata
    16861 cgcgtacaaa cttccacaaa aagttcgtcg cttggcactt aaatctgttt actcagaaaa
    16921 agttgctgaa aacaaatttg tagctgttaa ctcacttgaa ttcacagctc caaaaactgc
    16981 tgaatttgca aaagtacttg cagcattgag cattgattct aaagtccttg ttattcttga
    17041 agaaggcaac gaattcgcag ctctctctgc tcgtaacatc ccaggagtta aagttgcaac
    17101 tgcaacaact gcaagtgtac ttgacatcgc aaatgcagac aaacttcttg taactcaagc
    17161 agctatctct aaaattgagg aggttcttgc ataatgaatt tgtatgatgt tatcaaaaaa
    17221 cctgtcatca cagaaagctc aatgggccaa ctcgaagcag gcaagtatgt atttgaagtt
    17281 gacactcgtg cacacaaact cttgatcaag caagctgttg aagctgcatt cgagggtgtt
    17341 aaagttgcaa atgttaacac aatcaacgtg aaacctaaaa caaaacgcgt aggtcgttat
    17401 gtaggtcgca caaacaaagt gaaaaaagca atcatcacat tggctgctga ttcaaaagcg
    17461 atcgaattgt tcgctacagc tgacgctgaa taatcaaagg aggaattaac gtgggtatta
    17521 aagtttataa accaacgaca aatggccgtc gtaacatgac ttctttggac ttcgctgaaa
    17581 tcacaacaag cactccagaa aaaagcttgc ttgttgcttt gaagagcaaa gctggtcgta
    17641 acaacaacgg tcgcatcact gttcgtcacc aaggtggcgg tcacaaacgt ttctaccgtt
    17701 tggtagactt caaacgtaac aaagatggcg ttgaagcaat cgttaaaact atcgaatacg
    17761 atccaaaccg ttcagcaaac atcgctcttg tacactacac agacggtgtt aaagcttaca
    17821 tcattgctcc taaaggtctt gaagttggtc aacgcatcgt ttcaggtcca gaagcagata
    17881 tcaaagttgg taacgcactt ccacttgcaa acatcccagt cggtactgtt gttcacaaca
    17941 tcgagttgaa accaggtcgc ggtggtgagt tggttcgtgc tgctggtgca tctgcacagg
    18001 ttcttggtca agaaggtaaa tacgttcttg ttcgccttca atcaggtgaa gttcgtatga
    18061 tccttggtac ttgccgtgct actgttggca ctgtaggtaa cgaacaacat ggccttgtta
    18121 accttggtaa agcaggtcgt agccgttgga aaggtatccg tccaacagtt cgcggttctg
    18181 taatgaaccc taacgatcac ccacacggtg gtggtgaagg taaagcacca gttggtcgta
    18241 aagcaccatc tacaccatgg ggtaaaccag ctcttggttt gaaaactcgt aacaagaaag
    18301 ctaaatctga caaacttatc gttcgtcgtc gcaaccaaaa ataatctatt cttagttaca
    18361 tagcattcta tatcatccgc caactcggta gggtttgtta tggactgact attccagcac
    18421 ctcagtttga ttgaaatgcc agtttgacgc agtggttgat tgcagtttct agtagcttag
    18481 ctacgtcgaa acttctaatc aactgtgcgg gggcaggaca acgaacacgg taactggggt
    18541 tctgtccatc tcccaagccg ctgtggtaca tattttaaag gagaaaacac taaaatggga
    18601 cgtagtctta aaaaaggacc tttcgtcgat gagcatttga tgaaaaaagt tgaagctcaa
    18661 gcaaatgacg aaaagaaaaa agtaattaaa acttggtcac gtcgttcaac gatcttccca
    18721 agtttcatcg gttatacaat cgcagtttac gatggacgta aacacgtacc tgtatacatt
    18781 caagaagaca tggtaggtca caaacttggt gaatttgcac caactcgtac ttacaaaggt
    18841 catgctgctg acgacaagaa aactcgtcgt aaataattta ggaggaaaac acaatggcag
    18901 aaattacttc agctaaagca actgctcgca cagtacgtgt ttcacctcgt aaatcacgtc
    18961 ttgtcttgga taacatccgt ggcaaaagcg tagcagacgc aatcgcaatc ttgaaattca
    19021 caccaaacaa agctgcaggc attatcgagg gagttttgaa ctcagcaatc gctaacgctg
    19081 aaaacaactt tggtttggaa aaagctaact tggtagtcag cgaagcattc gcaaacgaag
    19141 gaccaacgtt gaaacgtttc cgtccacgtg cgaaaggttc tgcttcacca attaacaaac
    19201 gcacagctca tatcactgta gttgtggcag agaaataagg aggtaaaaac gtgggtcaaa
    19261 aagtacatcc aattggtatg cgtgttggca tcatccgtga ttgggatgct aaatggtatg
    19321 ctgaaaaaga atacgcggat taccttcatg aagatcttgc aatccgcaac tttatcaaaa
    19381 aagaattggc tgatgcgtca acatcaacaa tcgaaatcga acgtgctgta aataaagtaa
    19441 tcgtttctat ccacactgct aaaccaggta tggttatcgg taaagctggt agcaacgttg
    19501 atgcgcttcg tgctcaattg aacaaattga ctggtaagca agtacacatc aacatcatcg
    19561 aaatcaaaca acctgatttg gatgcacacc ttgttggtga gtcaatcgct cgtcaattgg
    19621 agcaacgtgt ggcattccgc cgtgctcaaa aacaagctat ccaacgtgca atgcgcgctg
    19681 gtgcaaaagg tatcaaaaca caagtttctg gtcgtttgaa cggtgcggat attgcccgtg
    19741 cagaaggcta ctcagaagga actgttcctc ttcatacact tcgtgcggat atcgactacg
    19801 cttgggaaga agctttgaca acttacggta aacttggtgt taaagtatgg atctaccgtg
    19861 gtgaagttct tccagctcgt aaaaacacta aaggaggtaa ataacaaatg ttagtaccta
    19921 aacgtgtaaa acaccgtcgt gaattccgtg gaaaaatgcg cggtgaagct aaaggtggaa
    19981 aacaagtaga ctttggtcaa tacggtcttc aagcaactac tagctcatgg attacaaacc
    20041 gccaaatcga agctgcccgt atcgctatga cgcgttacat gaaacgtggt ggtaaagttt
    20101 ggattaagat cttcccacac aaatcataca ctgctaaagc tatcggtgta cgtatgggtt
    20161 ctggtaaagg tgctcctgaa ggttgggtag ctccagttaa acgcggtaag gttatgtttg
    20221 aagtagctgg cgtttctgaa gaaatcgctc gcgaagcatt ccgccttgct ggtcacaaat
    20281 tgccagttaa agttaaattc gtaaaacgtg aagcagaata aggagaagac atgaaacttc
    20341 aagaaattaa agattttgta aaagaacttc gtggcctttc tcaagaagaa cttgctaaga
    20401 aagaaaacga attgaagaaa gaactcttcg aacttcgttt ccaagctgct gctggtcaac
    20461 ttgagcaaac tgctcgtttg aacgaagtga agaaacaaat tgcgcgtatc aaaactgtgc
    20521 aatctgaaac taaataatag attgggaaag gagaatttca atggaacgca ataatcgtaa
    20581 agttcttgtt ggacgcgtag tatctgacaa aatggacaaa acaatcacag ttgtagttga
    20641 aactaaacgt aaccacccag tctatggtaa acgtattaac tactctaaaa agtacaaagc
    20701 tcatgatgaa aacaatgttg ctaaagaagg cgatatcgtt cgtatcatgg aaactcgccc
    20761 actttcagct acaaaacgtt tccgtcttgt agaagttgtg gaagaggcag ttatcattta
    20821 atcaacctga aaggagaaaa ttgacatgat tcaaacagaa actcgtttga aagttgctga
    20881 caacagtggc gcacgtgaaa tcttgacaat caaagttctt ggtggttcag gacgtaaatt
    20941 cgcgaacatc ggcgacatca tcgttgcttc agtaaaacaa gctactcctg gtggtgcggt
    21001 taaaaaaggt gacgttgtta aagccgttat cgttcgtact aagacaggtg ctcgtcgtgc
    21061 tgatggttca tacatcaaat tcgatgagaa tgctgcagtt atcatccgtg aagacaaaaa
    21121 ccctcgcgga actcgtatct ttggcccagt ggcacgcgaa ttgcgtgatg gcggtttcat
    21181 gaaaatcgtt tcattggcac cagaagtact ttaatcaaat agttttagga aatttgttac
    21241 aaactaacga agtctgtgac gggtttagta aactagaaaa caaactaagt cccctggtta
    21301 aatcactaac cagggtgccc attagggcgt aagaaattat aggagaaatc aaatgtttgt
    21361 aaaaaaaggc gataaagttc gcgtaatcgc tggtaaagac aaaggcgttg aagcagttgt
    21421 cgtaacagca cttccaaaag taaacaaagt tattgttgaa ggtgttaaca tcgttaagaa
    21481 acaccaaaaa tcaaacagcg aaaaccctca aggcgctatc gttgaaaaag aagctccaat
    21541 ccatgtgtca aacgttcaag ttcttgacaa aaatggtgtt gcaggacgcg ttggttacaa
    21601 gtttgtagac ggcaaaaaag ttcgctacaa caaaaaatca ggcgaagtgc ttgattaatc
    21661 acgaaggaaa ggagaagtat aatggcaaat cgtttaaaag aaaaatatct taatgaagta
    21721 gttcctgctt tgactgaaca atttaactat tcttcagtta tggctgtgcc aaaagttgat
    21781 aagatcgttt tgaacatggg tgttggtgac gctgtttcta acgctaaaaa ccttgagaaa
    21841 gctgctcaag aattggcttt gatctcaggt caaaaaccac ttatcactaa agctaagaaa
    21901 tcaatcgccg gcttccgtct tcgtgagggt gttgcgatcg gtgcgaaagt aactcttcgt
    21961 ggcgaacgta tgtatgaatt cttggacaaa ttggttacag tttcacttcc acgtgtacgt
    22021 gacttccacg gtgtaccaac taagtcattt gacggacgtg gtaactacac acttggtgtg
    22081 aaagagcaat tgatcttccc agaaatcaac ttcgacgatg ttgataaaac tcgcggtatg
    22141 gatatcgtta tcgttacaac tgctaacact gacgaagaat cacgtgcatt gcttactggc
    22201 cttggtatgc cgtttgcaaa ataagaggga gagaataaat ggctaaaaaa tcaatgatcg
    22261 ctaagaacaa acgcccagct aagttctcta cacaagctta cacacgctgt gaaaaatgtg
    22321 gacgtccaca ctcagtttac cgcaaattca aattgtgccg tgtatgcttc cgcgacttgg
    22381 cttaccttgg acaaattcct ggcgtaacaa aagcttcttg gtaagataag atgtgagagc
    22441 gtttagcaac caacgcaaag ataggagatt ttacgacgaa gtttacttct aggaaaatct
    22501 atcttctttg ctaagcttgt agctcgaact caattaaaac atgatactaa gggcgtgacg
    22561 gacaaagtga aaataggaac tctagcgaag aacgcttgcg ttctagatag agttatcttt
    22621 ttcacacagg ccgtagcccg agttcaaatg ttaggatgca atagcatcat actagcaaat
    22681 tcaaactttg cattgcctat caggcacaca ttaactagca agtgatctaa tcaaactgct
    22741 agtaagagga gaaacaacaa atggttatga ctgacccaat tgcagatttt ttgacacgta
    22801 ttcgtaacgc taaccaagca aaacacgaag tgcttgaagt acctgcatca aacatcaaaa
    22861 aaggtattgc tacaatcctt aaaaacgaag gttttgtaaa aaacgttgaa ttcatcgaag
    22921 atgacaaaca aggcatcatc cgtgtattct tgaaatacgg accaaacggt gaaaaagtta
    22981 tcacaaattt gaaacgcgtt tcaaaaccag gtcttcgtgt ttactcaaaa cgtgaagata
    23041 ttcctaaagt tcttaacgga cttggtatcg caatcatctc aacatcagaa ggtcttttga
    23101 ctgacaaaca agctcgtcaa aagaacgttg gtggtgaggt tatcgcatac gtttggtaat
    23161 cttttagctt ccaatttcgt catgtgcctg tgttagagcg tcttgacata ctcaagtatg
    23221 gtctgcgtcg ctctgccttg tcacatttag aaattgaaac ctaaaagcat gatacaaatg
    23281 cgagaggacc agttcttcgt gcgtgttcaa atctgactta ctgattattt ggtatcatgc
    23341 aaccaaacta gaacaagatt cactaattga acacgcctac aactctttga aaaaagtgaa
    23401 aagtgtttca gaaatcctta ccttgttcgt cgtaaacgtc tgaactttct ctggccttcg
    23461 gtcaggaaag tcctagtcgt tctgacgagg gtgcgtctgc gaaatcaaag acttcggact
    23521 taccgcttat ttttcttcga gttgcttaac ggctttgtat cttgttcacc ccgtgaaaac
    23581 tagtcgcttg acggcttgat aatttaacag gagaattaaa aaatatgtca cgtattggta
    23641 ataaagtaat tacattgcct gctggtgttg agcttgctca aaacaacggc gtggtaactg
    23701 taaaaggacc taaaggggaa ttgactcgtg aattcccaac tgctattgaa atccgtgtgg
    23761 aaggtgcaga agtaactctt caccgtccaa acgattcaaa agaaatgaag actattcacg
    23821 gtactagccg tgctaacctc aacaacatgg ttgttggtgt ttctgaaggc ttcaaaaaag
    23881 aacttgaaat gcgtggtgtc ggttaccgtg ctcaattggc tggtaacaaa ttgacacttg
    23941 ctgttggtaa atcacatcca gatgaagtgg ttgcaccaga aggtatcaca tttgaagttc
    24001 caacaccaac acaaattgtc gtgtctggta tcaacaaaga agttgttggt caaacagcag
    24061 cttacatccg tagccttcgc gctcctgagc catacaaagg gaaaggtatc cgctacgttg
    24121 gtgaattcgt tcgccgtaaa gaaggtaaaa caggtaaata atagcttgct gaggtggctg
    24181 gaccacctgg ctgactattt ctcaaattgt ttcgtaagaa acagaatcat acattaagag
    24241 gtgaatattg tgatttcaaa accagataaa aacaaaatcc gccaaaaacg ccaccgtcgc
    24301 gttcgcggta aaatctctgg aactgctgct cgcccacgtt tgaacatttt ccgttctaat
    24361 acaggcatct acgctcaagt gattgatgac gtagcgggtg taacgctcgc aagcgcttct
    24421 actcttgata aagaagtttc aaaaggtact aagacagaac aagctgttgt tgtaggtaaa
    24481 ctcgttgctg aacgcgcggt agctaaaggt atttctgaag tggtctttga ccgcggtgga
    24541 tatctctatc acggacgtgt gaaagctttg gctgaatcag ctcgtgaaaa cggattgaaa
    24601 ttctaatagg gaggacacta agaaatggca ttcaaagata acgcagttga aattgaagaa
    24661 cgcgtagtag ccatcaaccg tgttacaaaa gttgttaaag gtggacgtcg tcttcgtttt
    24721 gcagctcttg tggttgttgg tgaccgtaac ggtcgcgtag gtttcggtac tggtaaagct
    24781 caagaagtac cagaagctat ccgtaaagca gttgaatctg ctaagaaaaa catgattgaa
    24841 gtaccaatgg ttggtacaac aatccctcac gaagttcgct cagaatttgg cggcgctcgt
    24901 gtattgttga aacctgcttc agaaggttct ggggttgctg ccggtggtgc aactcgtgcc
    24961 gtaatcgaat tggcaggtat cgcagatgtg acttctaagt cacttggttc aaacacacca
    25021 atcaacatcg ttcgcgcaac agttgaaggt ttgaaacaat tgaaacgtgc tgaagaagtg
    25081 gctgcacttc gtggcatctc agtttctgat ttagcataag aaaggagata ctcatggctc
    25141 aaattaaaat cactttgact aagtctccaa tcggtcgcaa accagaacaa cgtaaaacag
    25201 ttgttgcact tggacttggt aaattgaact cttcagttgt taaggaagat aacccagcta
    25261 tccttggtat ggtgaacgct atctctcact tggtaactgt agaagaagtt aaataatcta
    25321 acaaatagac taagtcgcat agagaaatct ttgcgacttc tagtccattt tacatattta
    25381 catacagcga gtttgtggag gaagactctt gtacttcaca ggcgctagca tataaaagag
    25441 gagaaataat aatgaaactt catgaattac aacctgctac aggttctcgt aaagtccgca
    25501 accgtgtagg tcgtggtaca tcatcaggta acggtaaaac atctggccgt ggtcaaaaag
    25561 gtcaaaaagc tcgtagcggt ggcggtgtcc gcccaggttt tgaaggtgga caaactccat
    25621 tgttccgtcg tcttccaaaa cgtggattta caaatatcaa cgctaaagag tacgcaatcg
    25681 ttaaccttga tcaattgaac gcttttgaag atggtgcaga agtaacacca gttgtgctta
    25741 tcgaagctgg aatcgttaaa gctgaaaaat caggaattaa aattcttggt aacggtgaat
    25801 tgacgaagaa attgacagtt aaagctgcta aattctctaa atcagctgaa gaagcaatca
    25861 cttctaaagg tggctcagta gaagtcatct aagaggtggc agcctatgtt ttttaaactt
    25921 ttaaaagatg ccttaaaggt aaaattggta cgtagtaaaa ttctatttac tatctttatc
    25981 ctttttgtct tccgcgtagg aacccacatt acagtaccag gagtaaacgc aaagagtctt
    26041 gaggccttgt caaaccttcc gttcttgaac atgttgagtt tggtttcagg aaatgctatg
    26101 cgtaatttct cagttttcgc actaggtgtt agtccttata ttacagcttc catcattgtt
    26161 caacttttac aaatggatat tttacctaaa tttgttgaat ggggtaagca aggtgaagtt
    26221 ggtcgtcgta aactaaatca agcaacacgc tacatttctt tggtactggc atttgttcaa
    26281 tcgattggta ttacagcagg ctttaatgct ttgtccggtg caaaattgac caatatgcca
    26341 ttaaattggc aaacatattt gttgattgga gcgattttga caacaggttc gattattgta
    26401 acctggctgg gggaacagat ttctgaaaag ggctatggta atggtacatc aatgatcatc
    26461 tttgcaggaa ttatttcatc gttaccagga acttttcacg agatttacat tgatcgcttt
    26521 gt
//
LOCUS       DBNFCK010000015        24932 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.015, whole genome shotgun sequence.
ACCESSION   DBNFCK010000015 DBNFCK010000000
VERSION     DBNFCK010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 24932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24932
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.015"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(75..1385)
                     /locus_tag="AC1PIW_000499"
     CDS             complement(75..1385)
                     /locus_tag="AC1PIW_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HIP6605463.1"
                     /translation="MTILATLGVLLAIVAIIYWTSKNLHVIIAAPLASLIIILTNQMN
                     VLEVMLGKEQSYMAGLAGFLINNFAIFMLGSILARYMEASGATQTIANSILKVMGKDS
                     PYKGLLAITLIASILTYGGVSIFVVIFTLLPLSRPLFKELNINWALFPIPVFLGAGTY
                     TMTTLPGAPSIQNVIPTKVLGTSLTAAPIISLAASLTLLVFGLLYMAYCLKKSLANGE
                     TYTEEEDETAVVVADKTPNLFLSVLPLFSLIGTIFLMSKTPNVLAIGLLVSILLSALI
                     FYPYLPNQKEILNAATTASIVPAFATSSTVAFGTVLTLSAGFAVIQEWIQQIPGSPLI
                     SLSVSTALISGIIGSSSGAIGIASSNFLPSYLEMGINPELLHRVVVVASAILTVMPQS
                     GVMITFHNLSKLSMKRGLKYSFILVTVGHLLALFVILLLAPLLY"
     gene            complement(1436..1924)
                     /gene="folK"
                     /locus_tag="AC1PIW_000500"
     CDS             complement(1436..1924)
                     /gene="folK"
                     /locus_tag="AC1PIW_000500"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HIP6605464.1"
                     /translation="MKHLAYLSIGGNMGNRQAYLQAALDKLASHPGCQLGLVSNIYET
                     PAWGKTDQADFLNLACQVYTDLSAQDFLSVFQNIEQELDRVRIEKWGQRTIDLDIIFW
                     DEEKIQEENLIVPHPYAHERAFVLKPLAEIASDYCHPILKKVVSDLLAELGEQKDIKI
                     FK"
     gene            complement(1921..2283)
                     /gene="folB"
                     /locus_tag="AC1PIW_000501"
     CDS             complement(1921..2283)
                     /gene="folB"
                     /locus_tag="AC1PIW_000501"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HIP6605465.1"
                     /translation="MDKISLNKCRFYGYHGAFKEEQVLGQIFTVDCDLFVDLTAASQT
                     DNLEDTVHYGMVFETIKAVVEGKPYILIEKVAGVICQEIFDQFPKVEKIRLAIYKENP
                     PIAGHYDSVGIELERERP"
     gene            complement(2276..3088)
                     /gene="folP"
                     /locus_tag="AC1PIW_000502"
     CDS             complement(2276..3088)
                     /gene="folP"
                     /locus_tag="AC1PIW_000502"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIP6605466.1"
                     /translation="MSIEQLANQVTIMGILNVTPDSFSDGGHYNEVESALVQVEKLLA
                     EGATVIDVGGESTRPGATFVSEEDEIARVVPIIRAIKENYDCLVSVDTYKTGTARAAL
                     EAGADILNDVWAGLYDGDMLALAAEYKVPIILMHNQKDESYADVVGEVKNFLAERAQA
                     ALDAGVSADQIWLDPGFGFSKNVQHNLDLLQGLEQITALGYPVLFGISRKRVVDYLLG
                     GNSLPTDRDQATAALSAWAVQKGCKMVRVHNVAANRDIVKVWDQLTSGGQHG"
     gene            complement(3097..3660)
                     /gene="folE"
                     /locus_tag="AC1PIW_000503"
     CDS             complement(3097..3660)
                     /gene="folE"
                     /locus_tag="AC1PIW_000503"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HIP6605467.1"
                     /translation="MSKQEQIEQTIYQLLELLGEDPNREGLLDTPKRVAKMYLEMFNG
                     LEEDPKDQFTAVFSEGHEEVVLVKDIPFHSMCEHHLVPFYGIAHVAYIPSKGRVTGLS
                     KLARAVEVASRRPQLQERLTHQVAHALQDALEPEGVFVMVEAEHMCMSMRGIRKPGSK
                     TVTTVALGKYKEDAILRRELLSMIHNK"
     gene            3828..4310
                     /locus_tag="AC1PIW_000504"
     CDS             3828..4310
                     /locus_tag="AC1PIW_000504"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIP6605468.1"
                     /translation="MTKKPVQLATICYIDNGKEFLLLHRNKKENDVHQGKWIGVGGKL
                     EPGETPQACAIREIFEETGLTVTKHALKGVITFPDFTPNTDWYTYVFKITGFEGSLID
                     CNEGDLEWVPYDQVLSKPTWEGDRHFQEWLLEDKPFFSACFRYDGDKLLDYSVDFYEE
                     "
     gene            complement(4358..5035)
                     /locus_tag="AC1PIW_000505"
     CDS             complement(4358..5035)
                     /locus_tag="AC1PIW_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HIP6605469.1"
                     /translation="MQLFNSIFSTANSNITLAQMSMVFGVSLSMGVLLAAVYKYKSNY
                     TKEFVITLSLMPALIAVIIALVNGNLGAGVAVAGTFSLIKFRSAAGSSKEMLAVLLAM
                     AIGLATGMGFLGLAVFMTVILSACILIFENISFAQVNQNRRHLLVTVPMNFDYDQFFE
                     KQFGTSCKQADLISLKYKKKKEALILEYQVLLDKKITDKRLVDTVLSAGPLDVVLNKQ
                     MPKKKYL"
     gene            complement(5022..5765)
                     /locus_tag="AC1PIW_000506"
     CDS             complement(5022..5765)
                     /locus_tag="AC1PIW_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HIP6605470.1"
                     /translation="MKTRIVQKQFKRKETKYIVDKKTFELLEKELRQYMMADEFATST
                     ITNIYFDNEDFDMIQDSLAKKNGREKIRMRVYDATPSESSQAFLEIKKKENKIGYKYR
                     LTSNPVSVANYIENGVIDSTIKDDKVTSELEMLRERYGTIKPKMYIYYDRVSYKGIED
                     KKVRLTIDKNLLYRDYDVDAMEGKFGKNLLDPTKVIMEVKVPEERPDWLVALLEKYQI
                     EKQSFSKYGNAYKLAHNITGEEVSKHAAV"
     gene            5958..6260
                     /locus_tag="AC1PIW_000507"
     CDS             5958..6260
                     /locus_tag="AC1PIW_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605471.1"
                     /translation="MYEFVLEYGSFPVKLIDGFVNNRSEIPDFLKEDEEMIARLNEIN
                     ELFHQLFLTIECKFDYIGKQFPDKIEQLRELYHPLADDLLAKYGNQIELKIEPFIL"
     gene            complement(6281..6547)
                     /locus_tag="AC1PIW_000508"
     CDS             complement(6281..6547)
                     /locus_tag="AC1PIW_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HIP6605472.1"
                     /translation="MNTQDIQEKFFREGKLIVIPKKLKSKQVLFAYLQEELAKKGSTF
                     TEKEVNAFLAEVYDDYAILRRYLVDYGYLSRDQYGLEYRIEEKR"
     gene            complement(6602..9001)
                     /gene="pheT"
                     /locus_tag="AC1PIW_000509"
     CDS             complement(6602..9001)
                     /gene="pheT"
                     /locus_tag="AC1PIW_000509"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIP6605473.1"
                     /translation="MLVSYKWLKELVDFDATSHDLSEKMSTTGIEVEGVEQKSAGLSK
                     LVVGQVVSAEPIPDTHLNICQVNVGEAITQIVCGAPNVKPGIKVIVALPGARIAGNYK
                     IKKGKIRGVESLGMLCSLSEIGVSDSVVPKVYADGIYHLPEDAVVGEPVFSYLDLDDE
                     IIELSITPNRADALSMRGVAHEVAAIYDSKVNFKPVVLEESDKKASDVIEVAIESDKV
                     TAYTARVIENVTIAPSPQWLQNLLMNAGIRPLNNVVDITNYILLYFGQPMHAFDFDKF
                     EGKKIVARQAKEGENLVTLDGEERDLIADDIVISVADKAVALGGVMGGASTEIDNSSK
                     TVVLEAALFDGKSIRKTSGRLNLRSESSSRFEKGINVATLTEAMDTAAAMIAELAGGQ
                     VLSGIVSAGSVDTSDVPVTATIDYVNRSLGTNLDYAQIADIFRRLGMETTGDASQFTV
                     AVPRRRWDIRIPADLVEEIARIYGYNNLPTTLPKEDGTAGELTLTQNIRRQVRSLAEG
                     AGLTEIISYALTTPDKAVEFVARPTTVTELMWPMTVDRSALRQNMVSGMLDTVAYNVA
                     RKNKNLALYEIGKIFEQSGNPKEDLPQEINKFALVLTGLVAEKDFQTPAVPVDFFHAK
                     GILEAIFDHYKLAVDFVATSEIAALHPGRTAEIHLNGAVIGFVGQVHPQTAKDYGIPE
                     TYVAEINLDAIEEALQPAQPFTDISKFPAVSRDIALLLNSDVKHQDVLDAIAGAGVKR
                     LTKVTLFDVYAGSNIEVGKKSMAYNLTFQNPEDSLTDEEVAKYMEKISKSLETLGAEI
                     R"
     gene            complement(9003..9524)
                     /locus_tag="AC1PIW_000510"
     CDS             complement(9003..9524)
                     /locus_tag="AC1PIW_000510"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6605474.1"
                     /translation="MQIDPIRLEEVSVLQELAKQTFAETFAHDNDPKELEAFFEESYS
                     LEVLTSELSDPNCQHYFLRIDHQIAGYLKLNKGSAQTEQELENAFEIQRIYLLQAYQG
                     RGLGKVLFEFALEKAEQSSCDWVWLGVWEHNYKAQKFYSKYGFEKFGQHEFAVGDKID
                     VDWLLKRPLHQKG"
     gene            complement(9726..10769)
                     /gene="pheS"
                     /locus_tag="AC1PIW_000511"
     CDS             complement(9726..10769)
                     /gene="pheS"
                     /locus_tag="AC1PIW_000511"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIP6605475.1"
                     /translation="MSNIEQQLAELSQTTLEKLKEIQHQGEKELQDLRVAVLGKKGSL
                     TDLLKGLKDLSNDMKPIVGKQVNEVRDVLTTAFEETAQKVAAAKIQQQLASETIDVTL
                     PGRQVKVGKRHVLTQTSEEIEDIFLGMGFQIVDGFEVEKDYYNFERMNLPKDHPARDM
                     QDTFYITEEILMRTHTSPVQARTMDQHDFSKGALKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGENVSMGDLKGTLEMIIKKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     DGCNVCKKTGWIEILGAGMVHPQVLEMSGIDSTKYSGFAFGLGQERIAMLRYGINDIR
                     GFYQGDVRFSEQF"
     gene            complement(11225..12949)
                     /locus_tag="AC1PIW_000512"
     CDS             complement(11225..12949)
                     /locus_tag="AC1PIW_000512"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HIP6605476.1"
                     /translation="MPRLVDLRKKPYCLNDEQITWVENTLAHLTDEEKIGQLFVNLFF
                     FGGDAFSGNNLSNQELMNRYHIGGARYMNGSPEQVQGLINELQSYSKVPLLVAANCDS
                     GGDGAVKGGTYISSGAQSEASRDPRIPYLAGLVSAREETALGVNVNFDPCVDIVQNWR
                     NTIVNTRAYGTNADDVITYTSAYLEGLTAERDVIQCIKHFPGDGKEERDQHLVLGVNE
                     LSPEEWDKSFGRVYRHHIEAGVEMIMAGHIALPYYQQRLNPSLTDEDILPATLAPELI
                     QDLLKEKLEFNGLVITDASHMLGMTAAMRREDYVPAAIAAGCDMFLFFNNLEEDFEFM
                     LNGYRKGVITDERLHDALRRILGLKAKLNLHIKQAEGTLLKSADELAIIGCEEHLAWQ
                     REAADKAITLLKDTQKNLPISPETHRRIRLYYLEGEKEGIMAASTEVVDNLKAALEAR
                     GYEVTVNDGTTRIKGKTLQYREEVDLALTVANVIGYGAQNNYRIQWKTAMSNEVPWYV
                     HEVPTIFISLNYTTHLHDVTMVKTAINAYHHNKNTIESLLDKLEGKDAFFGVPNENVW
                     ANKWQAKL"
     gene            complement(12960..14750)
                     /locus_tag="AC1PIW_000513"
     CDS             complement(12960..14750)
                     /locus_tag="AC1PIW_000513"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HIP6605477.1"
                     /translation="MTHLVDLRKKPYSLDEEAISWIEETIAQMTLDEKIGHLFVNMGS
                     QRTEDYLTGVLNDYKIAAVRYNPGPAADIWEQNYILQTKSKIPLLIAANTESGGNGAV
                     TDGTKIGDEIKIAATTDPRYAYEMGKVAGLEAAAVGCNASFAPIMDLSRNWRNPIIAN
                     RTWGANVDQVIELSKEYMRGIMEHGIVPFAKHFPGDGIDERDHHLSFASNPMTKSEWM
                     ETFGRIYGEMADAGLPGIMAGHIHLPNVEKEMHPERELDDMLPASLNKTLLDELLRGE
                     LGYNGAIVTDASHMIGMTASMPRRELLPTAIEAGCDLFLFFNDPEEDLQWMKEGLENG
                     LLSEERLHDALRRTLGLKAKLGLHLFEGRRQEIMLPKEEALALIGRDEAKQLAKEVAD
                     KAITLVKAKQEGIFPVNPERYKRILLVEVDGYKGGFGAMINAGKKRAADTLKELLEAR
                     GHQVSIWENTEARIAKLPEEERPAAIANVYASKRPIAEITDNYDLIINLVDVNSGGTT
                     QRIIWPAAKGTPDQPFYVHEIPTIIVSVQHPFALADMPQVATYINAYDGLPVTLEALV
                     AKLAGESAFTGVSPVDAYCGLVDTQIWRGK"
     gene            complement(14797..15597)
                     /locus_tag="AC1PIW_000514"
     CDS             complement(14797..15597)
                     /locus_tag="AC1PIW_000514"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIP6605478.1"
                     /translation="MMKMEYVFCVDSDGCAMDTMTYKHKLFFGPLAAEVFGVEDKEPF
                     LAEWNRVNLYSRERGINRFVGLVKGLEFAGVTGIDNLKNWVATTDSLSNASLERLIEE
                     QPSKDLELALKWSTQVNQAIKHYSGPVLAFIGVHKGLEKLSQLGKVYVVSSANKEAVE
                     EEWTDQGLMDFVTELYCQDRGKKEDVIKLLIEEGYCPNKIMMIGDSPGDLKAAELNGV
                     HFYPILVGREMQSWADLTETIADDFAHQAFTGEKETELIQAFWNNLDD"
     gene            complement(15597..16436)
                     /locus_tag="AC1PIW_000515"
     CDS             complement(15597..16436)
                     /locus_tag="AC1PIW_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIP6605479.1"
                     /translation="MTRVIEFKDKVVVITGAGGVLCGYLAKEFAKAGAKVALLDLNQA
                     AAQVFVDEIVAAGGSAKAYKSNVLSKENLEEVRQQVLADFGPVDILINGAGGNNPKAT
                     TDNEFHEVGLPADTKTFFDLDEAGINFVFNLNYLGTLLPTQVFAQDMIGRSGANIINI
                     SSMNAFTPLTKIPAYSGAKAAISNFTQWLAVHFSKVSIRCNAIAPGFLVTNQNRGLLF
                     DESGQPTARANKILTNTPMGRFGESEELVGGVFFLADENLASFVNGVVLPIDGGFSAY
                     SGV"
     gene            complement(16439..17539)
                     /locus_tag="AC1PIW_000516"
     CDS             complement(16439..17539)
                     /locus_tag="AC1PIW_000516"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HIP6605480.1"
                     /translation="MKMSFRWYGKKDPVTLEEIKAIPGMQGIVTAVYDVPVGQAWPLE
                     NILELKKMVEEAGLEITVIESIPVHEDIKQGKPNRDALIENYKTSIRNVGAAGIPVVC
                     YNFMPVFDWTRSDLHHPLPDGSTSLAFLKSDLAGVDPVADDLNLPGWDSSYSKEEMKA
                     IIENYRQNISEEDLWANLEYFIKAILPTAEEAGVKMAIHPDDPPYGIFGLPRIITGQE
                     AVERFLNLYDSEHNGITMCVGSYASDPKNDVLAMTEYALKRNRINFMHTRNVTAGAWG
                     FQETAHLSQAGDIDMNAVVKLLVDYDWQGSLRPDHGRRIWGDQTKTPGYGLYDRALGA
                     TYFNGLYEANMRAAGKTPDFGIKAKTVGTKEG"
     gene            complement(17554..18954)
                     /gene="uxaC"
                     /locus_tag="AC1PIW_000517"
     CDS             complement(17554..18954)
                     /gene="uxaC"
                     /locus_tag="AC1PIW_000517"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HIP6605481.1"
                     /translation="MSFNDKNFMLKNEPAKELYGKIAELPIYDFHCHLDPKEIFEDKV
                     YEDIVDLWLGGDHYKWRLMRANGISEEEITGSASKLDKFKAWARTLERAFGNPLYHWS
                     HLELRQVFGVEELLTEENAERLYHQLNAYLQEHQVSPRKLIADVRVTFIGTTDHPLDS
                     LEWHKRLAEDSTIDTVVAPTFRPDEAFIEHRNFNPFIKRLEEVTGVAVRDFASFVEAL
                     GQRVSYFAQHGCRASDISFTAISYEEATLEELDEILLARLAGKEAGQSSINKWQTAIF
                     RELCRLYKKHGFVTQVHFGALRNNHTGLFEKLGADVGVDSIGDQTCLTVNLNRFLDNL
                     VKENALPKMIWYNLNPSYNIALANTLANFQSNEEGVRSQLQFGAGWWFNDTKLGMIDQ
                     MNAYAEQGMLANFVGMLTDSRSFLSYQRHDYFRRILATYVGQWIVDEEVPEDYNRLGQ
                     FVEAISYYNAKEFFEQ"
     gene            complement(18956..19576)
                     /locus_tag="AC1PIW_000518"
     CDS             complement(18956..19576)
                     /locus_tag="AC1PIW_000518"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - (R,S)-4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HIP6605482.1"
                     /translation="MLKQLKENYFFAVIRGKDEEDAKEIARHAILGGIRNIEITFSTP
                     NAATVIKELQEEFSDDSSVVIGAGTVMNLKLAQAAIDAGASFLVSPHFDKEIQDLAQE
                     AEVFYFPGCATATEIVTASQAGCPIIKLFPGGVLGPGFIKDIHGPVPDVNLMPSGGVS
                     VENVADWKKAGACAVGVGSALASRVQAEGYESVTTIARSFVAALEG"
     gene            complement(19699..20415)
                     /locus_tag="AC1PIW_000519"
     CDS             complement(19699..20415)
                     /locus_tag="AC1PIW_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIP6605483.1"
                     /translation="MAKPLVEKASERLLELILERGYEVGDKLPNEYELAQDLEVGRST
                     VREAVRSLATRNILEVRQGSGTYISSKKGVSEDPLGFSLVRDRVKLTTDLFEVRYLLE
                     PRIAERVAQFASDEDIARLEEIVLAIEEAVAAGDTKHLELDVKFHCMLAEMSGNLAMT
                     SLMPVINQSIHLINENYSNRQMKSDSLQAHRNILMAIKQRHPVAAYDSMLAHIISVRQ
                     VVLSEWFDKDMTLHGLNDHK"
     gene            complement(20461..22260)
                     /gene="uidA"
                     /locus_tag="AC1PIW_000520"
     CDS             complement(20461..22260)
                     /gene="uidA"
                     /locus_tag="AC1PIW_000520"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HIP6605484.1"
                     /translation="MLYPIQTKTRSVYSLNGIWKFKQGNNDVHTLLDTDEVMVVPSSF
                     NDVVVDKDKRRFVGDNWYELMVALPVVADDEELVVRFGSVTHQAKVYADGQLIREHKG
                     GFTPFECLIPSNLYDADQFRLTVCANNELNYTTLPVGNYSEEVDENGQIVKTVKENFD
                     FFNYAGIQRTVHLYKRPQNRIEDIVIRTELNQDLTQAVVNVDVKTVGQYDSIRMSILD
                     QEGQEVGLLENGQMVIEHPRLWEVLDAYLYTAKVELVDGENLLDTYSEQFGIRSVAVE
                     NGQFFINGKPFYFKGFGKHEDTFINGRGFNEAANLMDLNLLKDIGANSFRTSHYPYSE
                     EMMRLADRLGIVVLDEVPAVGLFQLFNAALNLTGDSEEAKNTWEVMQTKEAHELVIDE
                     LIARDKNHPSVVMWVVANEPAGHEKGARAYFEPLIQRMRDRDPSKRPVTLVNIMTATP
                     DKDEVMDLVDVVCLNRYYGWYVAHGDLKAAEKGLRQELETWQKLYPDKPILITEYGAD
                     TLPGLHSMWDIPYTEEFQVDYYDMNHCVFDSIPNLVGEQVWNFADFETTVGIIRIQGN
                     HKGLFSRNRQPKQIVREIKKRWTAIPNYHYKRK"
     gene            complement(22276..23304)
                     /locus_tag="AC1PIW_000521"
     CDS             complement(22276..23304)
                     /locus_tag="AC1PIW_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIP6605485.1"
                     /translation="MKKILAFGEVLLRLSPPHYQTLTQATSLDCQFGGSELNVLASLA
                     QLGHSVELVTALPENEVGKMAEHFLFARQIGQKHLIRKKGRLGIYYYQKGFSLRPSQV
                     TYDRDYSAFSLSKEEEYDLEGMFDGVDWFHVSGITVALNPDIYRLAFRLMEMAKRQGV
                     KVSFDLNYRESLWSSFEEAREKLSPFVGLADICFGLEPIQLFNESGKDVKDELGLKRP
                     YENKEVLLAVVKGLAETYSLQSLAFTQREMTYNNEYLLKAYLYQDGILYETEKESIQV
                     LDRVGTGDAFTAGIILGYLQEKTPQEIVDLGMASFQFKHTIEGDVNVISPSDIALLLK
                     KGSREIKR"
     gene            complement(23360..24913)
                     /locus_tag="AC1PIW_000522"
     CDS             complement(23360..24913)
                     /locus_tag="AC1PIW_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIP6605486.1"
                     /translation="MEKHVEQRVEDVTYNKAKLWQIILFAMNNSSTNIYLVAFTFVTY
                     LSTGVLGLAALFVSQLMGYIRIFDGFIDPTIGVIIDKTDTKFGKYRPILVLGNIITAA
                     SLFMMLGLSGVSESLRLPLFILVLIIHKIGYSLQQTITKAGQVALTNDPKQRPIFNIV
                     DGIATTVLMTGSQVVVSGTLVPKHGGFTRSFFQELFLGVVVISAVLGILAVIGIWEKD
                     NKKYFGLGEKTQETKLKDYWKVIKGNKPLQVLSISAAFVKFVAQLFGDSVIRVMLYGI
                     IFGDYALSGKMSMLLILPGILVTVGAAQLARRKGLRFAYITALQLGIAGLVTLAAMLF
                     SAKPGMLDLTNWNLYTIGFFVVYIIARYASSAPSGLVLTMGADITDYETSVSGRYLSG
                     MIGTIFSLTDSIASSFAPMVVGFVLAGIGFGKEYPTVETAFSTDLRMALIVLLTVIPA
                     VVLSVSLFLMKFYKLDREEMARIQEKIHVMKAAKDEERVRAIAKNVPLSDMDYVDVTQ
                     YKVDQYDDK"
BASE COUNT         7172 a         5945 c         4719 g         7096 t
ORIGIN      
        1 taaatatttg agtagaggtt ttaactgaat gatattgccc attggcaggg cttttatttg
       61 tttgttaatc aatcctaata tagcaaaggt gccaacagta aaatcacaaa taatgctaac
      121 aaatgcccaa ctgtcactag gataaacgaa tacttgaggc cacgtttcat acttaatttc
      181 gaaagattgt ggaaggtaat catcacacca gactgaggca taactgtcaa aatagccgat
      241 gcaactacta ctacacgatg cagcaactcc ggattaatcc ccatttccaa ataggaagga
      301 aggaaattac ttgaagcaat acctatcgca ccagaagatg aaccgataat accactaatc
      361 aatgctgtcg aaacagataa actgattaga ggtgatcctg gtatttgttg aatccattct
      421 tgaatgactg caaaacctgc tgacaaagtt aacacagtcc caaaggctac tgtactagaa
      481 gttgcaaaag caggcactat agatgccgtt gtcgcagcat tcaaaatttc tttttgattt
      541 ggcagatatg gataaaaaat caaggctgat agaaggatag agaccaacaa tccgattgcc
      601 aggacattcg gagttttact cattaggaaa atcgtcccaa taagactgaa tagaggcaag
      661 actgataaaa atagatttgg tgtcttatca gctaccacta ctgcagtttc atcctcttct
      721 tccgtatacg tctctccatt tgctagactt ttcttgaggc agtaggccat gtagagcaag
      781 ccaaatacta atagtgtcaa gctggctgcc aaactgataa tgggtgccgc ggtcaagcta
      841 gttcccaaaa cttttgtcgg aatgacattc tggatggaag gtgccccagg aagagttgtc
      901 atcgtatagg tacctgctcc caaaaagaca ggaatcggaa aaagtgccca gttgatattc
      961 aactctttaa agagggggcg tgaaagggga agcaaggtaa aaataactac aaaaatactc
     1021 acaccaccat aggtcagaat ggaagcaatc aaagtgatag ctaacaagcc cttataggga
     1081 ctatcctttc ccattacctt caaaatgcta ttggcgatcg tctgcgtagc tccacttgcc
     1141 tccatataac gtgccaagat agaccccaac ataaagatgg caaaattatt aatcaaaaaa
     1201 cctgctaagc ccgccatata agactgctct ttgcccaaca taacttccaa aacattcatc
     1261 tgattggtca gaatgatgat taaactagcg agtggtgcag caatgataac atgtaaattt
     1321 tttgatgtcc aataaataat ggctacaata gccaataaaa ctcctaaagt tgctaaaatt
     1381 gtcataaaac tcctttatct attctatcct ctaataaacc tagtatcaga ggcaactatt
     1441 tgaaaatttt tatatctttt tgttccccta gttctgcaag caagtccgat accacttttt
     1501 tcaaaattgg gtgacagtaa tcagaagcaa tctcagcaag aggtttgaga acaaatgctc
     1561 tttcatgggc gtagggatgg ggaacaatca gattctcttc ttgaatcttc tcctcatccc
     1621 aaaaaataat atccaaatca atggtccgtt gtccccattt ttcaatccgt actcggtcca
     1681 actcttgttc gatgttctgg aaaacgctta gaaaatcctg agcagacaaa tcagtataca
     1741 cttgacaggc tagattgagg aaatcagcct ggtcggtctt gccccaagct ggagtttcat
     1801 aaatgttgga aactaagcca agctggcaac ctggatgact ggctaatttg tctagggctg
     1861 cctgtaaata ggcttgtcga ttgcccatat tccctccgat actgagatag gctagatgct
     1921 tcatggtcgt tcacgctcca attcaattcc gacagaatca tagtgtccag caatgggcgg
     1981 attttccttg taaatggcca agcgaatctt ctccaccttt ggaaattgat caaaaatctc
     2041 ctgacagatg acacctgcca ccttttcaat gagaatatag ggcttgcctt ccacaactgc
     2101 tttaatggtt tcaaagacca taccatagtg aactgtgtct tctaaattgt cagtttgtga
     2161 agctgctgtc aggtcaacaa acaaatcaca gtcaactgtg aaaatttgac caagaacttg
     2221 ttcttctttg aaggcaccat ggtagccgta aaagcgacat ttattgagag aaatcttatc
     2281 catgttgacc tccagaagtc aattggtccc aaaccttaac gatatcacgg ttggcagcga
     2341 cattgtgaac acggaccatt ttacagcctt tttggactgc ccaggcagac aaggctgccg
     2401 ttgcttgatc gcggtcagtc ggtaaactat tgcccccaag cagataatcc accacgcgct
     2461 tacgagatat tccaaataga acgggataac caagggcagt aatctgttcc aaaccttgta
     2521 agagatcaag gttatgttgg acattctttg aaaagccaaa tcctggatcc aaccaaatct
     2581 ggtctgcgga aacaccagca tcaagtgctg cttgggcacg ctcagctaga aagtttttga
     2641 cctctccaac aacatccgca tagctttcgt ctttctggtt gtgcatgaga ataattggta
     2701 ccttgtactc cgcagccagg gccaacatat ctccatcata aagtcctgcc cagacatcat
     2761 ttaaaatatc tgcgcctgct tccaaggctg cacgtgcagt tcctgtttta taggtatcga
     2821 cgctgacaag acagtcatag ttttctttga tagcgcggat aatcggaacc acgcgagcaa
     2881 tctcgtcttc ttcagacaca aatgttgcac ctggccgagt ggactcacca ccaacatcaa
     2941 tgacggtagc accctcagcc aacaactttt ctacttgtac caaggcagac tccacctcat
     3001 tatagtgacc accatctgaa aatgaatctg gcgtcacatt gagaatcccc ataatggtta
     3061 cttgattcgc taactgttcg attgacatag aacctcctat ttattgtgaa tcatggacaa
     3121 gagttcacgg cgcaagatgg catcttcctt gtatttgcca agagcaactg tggtcactgt
     3181 cttactacct ggcttgcgaa taccacgcat gctcatgcac atgtgctctg cttcgaccat
     3241 gacaaaaaca ccttctggtt caagggcatc ttgaagggca tgggcaacct gatgggtcaa
     3301 gcgttcctgc aattgagggc gacgacttgc tacctccact gcacgcgcca atttgctaag
     3361 acctgtcaca cgccccttac tcggaatata agctacatgg gcaatgccat aaaagggtac
     3421 caaatggtgt tcacacatag aatgaaaagg aatatccttg accaaaacaa cctcttcatg
     3481 accttctgaa aagacagctg taaattggtc tttgggatcc tcttctaggc cgttaaacat
     3541 ttctaaatac atcttagcaa cccgtttagg cgtatctaat aaaccttcac gattcgggtc
     3601 ctcacccaat aattccaata attgataaat tgtttgttcg atttgttctt gttttgacat
     3661 agtgcatcct aaatagtttg atgttcttat tatacctcaa aaactgtttt cagaaaagag
     3721 ctttcagtat gctaatccca aaactgtccc ctaccagagc accaagcagg tgtccctact
     3781 cccgcctttg tgatataata ggaacaaact aactataagg agctactatg accaagaaac
     3841 ctgtccaact ggctacgatt tgctacattg ataatggcaa agaatttctc ctgctccatc
     3901 gcaataaaaa agaaaacgat gtccaccagg ggaaatggat tggggtcggt ggtaagctgg
     3961 aacccggtga aacaccgcag gcttgtgcca ttcgagaaat ctttgaggaa actgggctta
     4021 ctgtaacgaa gcatgctcta aaaggagtga taaccttccc agactttact ccaaacactg
     4081 actggtacac ctatgtcttt aagataactg gttttgaagg aagtcttatt gactgcaatg
     4141 aaggtgactt agagtgggtt ccctatgacc aagtcttgtc caaaccaact tgggagggcg
     4201 accggcattt tcaagaatgg ctattagagg acaaaccatt tttttccgct tgcttccgct
     4261 atgatgggga caaattgctt gattatagcg ttgattttta tgaagaataa gctaataaaa
     4321 aacgaaaaag ccttggaaat ccagggcttc tttgatatta caaatacttc ttcttcggca
     4381 tttgcttgtt caatacgaca tccaaaggac cggctgacaa tactgtatct accaagcgtt
     4441 tatctgtaat ttttttatcg aggagaactt gatattctaa gatcagcgct tcttttttct
     4501 tcttgtactt gagggaaatg agatccgctt gcttgcagga tgtaccaaat tgtttctcaa
     4561 aaaattggtc gtagtcaaaa ttcattggca ctgttaccaa gagatgacga cgattttgat
     4621 tgacttgagc aaagctgatg ttctcaaaaa tcaaaataca agcagatagg atgaccgtca
     4681 taaagacagc caaacctagg aagcccattc ctgtcgctag accgatagcc atcgctaaga
     4741 ggactgcaag catttccttc gaagaacctg ctgctgagcg gaatttaatt agactgaaag
     4801 tccctgctac cgcaacaccc gctcccaagt taccattaac taatgcaatg ataacagcaa
     4861 tcaatgccgg catcagactt agtgtgatga caaattcctt tgtataatta gatttatatt
     4921 tataaacagc cgctaagagt acccccatgg atagactaac tccaaaaacc atggacattt
     4981 gcgctaaggt aatattgcta ttggccgttg aaaagatact attaaacagc tgcatgtttg
     5041 cttacttcct ctccagtgat gttgtgggca agcttatagg cattgccata ttttgaaaat
     5101 gattgctttt cgatttggta tttttcgagc agggctacca accagtctgg tcgttcttct
     5161 ggaactttta cttccatgat tactttggtt gggtctaata ggttcttacc aaatttacct
     5221 tccattgcat ccacatcgta gtctcgataa agtaagttct tatcgattgt gagacgaact
     5281 tttttgtctt cgattccttt ataggatacg cgatcgtagt agatatacat cttcggctta
     5341 atagtaccgt agcgttctct caacatttcg agttctgagg ttaccttatc atccttgatt
     5401 gtggaatcaa tcactccatt ttcgatataa tttgctacag aaacagggtt tgaagttaaa
     5461 cggtatttat agccaatttt attttctttt ttcttgattt caagaaaagc ttgactcgat
     5521 tcagatggtg tcgcatcata gacacgcatc cgaattttct cacgtccatt ctttttggca
     5581 agtgaatctt gaatcatatc aaaatcttca ttatcaaagt agatattggt aatcgttgat
     5641 gtcgcaaact catctgccat catgtactga cgaagttcct tttctaaaag ttcaaaagtt
     5701 tttttatcaa cgatatattt agtctcttta cgcttaaact gcttttgtac gattcttgtt
     5761 ttcattattg tgcctcttct atcctaaatt ttgacaggtc cctgtcttgt ttacaagata
     5821 tagtttaccg acctagcttt aaatgaaact gaaaataaaa ttaaacaaaa cttaaactgc
     5881 aataaagttg aaattctttt gtcacattct aatctatgct acaatagtcc tatcaatagt
     5941 aaggcaagga gaaacccatg tacgaatttg tcctagaata cggctctttc cccgtaaaac
     6001 tcatcgacgg ttttgtcaac aatcgctcag aaatccctga ttttctcaaa gaagacgaag
     6061 aaatgattgc ccgcttgaat gagattaacg aactcttcca ccaacttttc ttaaccatcg
     6121 agtgcaagtt tgactacatc ggcaaacaat ttcctgacaa gatcgaacaa ctacgagaac
     6181 tctatcatcc cctggcagac gatttactag ccaagtatgg caaccagata gagttgaaga
     6241 ttgaaccctt tattttgtaa gacaaaagag atgaccgcaa tcatctcttt tcttctattc
     6301 tgtattccaa accatattgg tcacgcgaca agtagccgta gtccaccaag tagcgtctca
     6361 atatagcata atcatcatag acttcagcta gaaaggcatt gacttctttt tctgtgaagg
     6421 tagagccttt cttagccaac tcttcttgta aataggcaaa taaaacttgc ttacttttta
     6481 attttttagg aataacgata agcttgcctt ctcgaaaaaa tttttcttga atatcttgag
     6541 tgttcatact tccctccaaa aaatcccagc ccgctgacta ggatttcaca agatttacat
     6601 gttaacgaat ttcagctcca agtgtttcga gcgacttact gattttctcc atgtacttag
     6661 ccacttcctc atccgtcaag ctatcctcag gattctggaa ggtcaggtta taagccatag
     6721 acttcttacc aacctcgata ttgctgccag catagacgtc aaagagagtc accttggtca
     6781 agcgtttcac gccagcacca gcaatggcat ccaggacatc ctgatgtttg acatcgctgt
     6841 tcaaaagcag ggcaatatca cggctaaccg ctgggaattt agagatgtcg gtaaatggct
     6901 gagcaggctg gagggcttct tcaatcgcat ccagattgat ttcagccaca taagtttcag
     6961 gaataccgta gtccttagcc gtttgagggt gtacctgacc gacaaagccg atcacagccc
     7021 cattcaaatg gatttcagct gtacgacctg ggtgcagggc agcaatctca ctagtcgcca
     7081 caaagtcaac tgccaacttg taatgatcaa agatagcttc caaaatccct ttggcatgga
     7141 agaaatcaac tggtacagct ggtgtctgga agtctttctc agcaaccaaa ccagtcaaaa
     7201 caagggcaaa cttgttgatt tcttgtggca ggtcctcttt tggattgcca gactgttcaa
     7261 agatcttacc gatttcgtaa agagccaaat tcttgttctt acgagccacg ttgtaggcca
     7321 ctgtgtctaa cataccagac accatgttct gacggagggc agagcggtca actgtcatcg
     7381 gccacatgag ttcggtgact gtggttggac gagccacaaa ctccacagcc ttgtcaggcg
     7441 ttgtcaaggc ataggaaatg atttctgtca gacctgcacc ttcagccagg ctacgtactt
     7501 ggcgacggat gttctgggtc agggtcaact cgcctgctgt tccgtcctct ttcggaagag
     7561 tggtcggcag attattgtaa ccatagatac gagcgatttc ctctaccaag tctgctggaa
     7621 tgcgaatgtc ccaacgacga cgcggtactg ctactgtaaa ctgacttgca tcgccagttg
     7681 tttccatacc taaacgacgg aaaatatctg cgatttgggc atagtcgagg ttggtcccaa
     7741 gcgagcggtt aacgtaatca atggttgctg tgactgggac atccgaagtg tccacgctgc
     7801 ccgcagaaac gatgccagac aagacctgac cgccagccag ttccgcaatc atagcagccg
     7861 ctgtgtccat agcctccgtc aaagttgcta cgttgatgcc tttttcaaag cgagaagagg
     7921 actcagaccg aaggttgagg cgacctgagg tcttgcggat agacttgccg tcaaagaggg
     7981 ctgcttccag cacgacggtc ttagagctat tgtcaatctc tgtactagca ccacccatga
     8041 caccaccgag ggcaactgcc ttgtccgcaa cggaaatgac gatgtcatcg gcaatcaagt
     8101 cacgttcttc gccgtcaaga gttaccaagt tctcaccctc ttttgcctga cgagccacga
     8161 ttttcttgcc ttcaaattta tcgaaatcaa aggcgtgcat tggctgaccg aagtagagca
     8221 agatgtagtt ggtgatgtcc accacattgt tgagcgggcg aatacctgcg ttcatgagca
     8281 ggttttgcaa ccactgcggg ctcggtgcga tagtcacatt ttcaataaca cgggccgtgt
     8341 aggcagtcac cttgtctgac tcaatggcca cttcaatcac atcgctggct ttcttgtcgc
     8401 tttcctccaa gaccactggc ttgaagttga ccttgctgtc atagatagct gccacttcgt
     8461 gagccacacc acgcatagag agggcgtcgg cacggtttgg tgtgatggac aactcaataa
     8521 tctcgtcgtc gaggtcaaga tatgaaaaga ctggctcacc cacgactgca tcttctggca
     8581 ggtggtagat accgtccgca tagacctttg gcacaactga gtcggacacg ccgatttcgc
     8641 tcaatgagca gagcatgccc aaggattcaa ctccgcggat tttgcctttc ttaatcttgt
     8701 agttgccagc gatacgagct cctggcagag caacgatcac cttgatacct ggcttgacat
     8761 ttggagcacc gcaaacaatt tgcgtgattg cttcgcccac atttacctga caaatattga
     8821 ggtgagtatc tggaattggc tcagcagaca caacctgacc gacaaccaac ttagaaaggc
     8881 cagcactctt ttgctcaacc ccttctactt caattcctgt tgttgacatt ttttcagaca
     8941 agtcatggct tgtcgcatca aagtcaacga gttcttttaa ccatttgtaa cttactaaca
     9001 tattatcctt tctggtgcaa aggccgtttc agtagccaat ccacatcaat cttatcaccg
     9061 accgcaaatt catgctggcc aaatttttca aatccgtatt tggaatagaa tttctgagcc
     9121 ttgtaattat gttcccaaac tcccagccag acccaatcac aactagactg ctctgctttt
     9181 tccaaagcaa attcaaacaa gaccttccca agaccgcgac cttgataggc ctgcaagaga
     9241 tagatacgct ggatttcaaa ggcattttcc aactcttgct ccgtctgggc agaacctttg
     9301 ttgagcttga gataaccagc tatctgatga tctattctta gaaaatagtg ctggcagttg
     9361 ggatccgata actcactagt caagacctcc aaggaataag attcctcaaa gaatgcctcc
     9421 agttccttcg ggtcattatc gtgagcgaag gtctctgcaa aagtctgctt ggccaactcc
     9481 tgcaaaacgg aaacctcttc caaacgtata ggatcaatct gcatctcatt gcctctcatt
     9541 tctaactgga ctaggaaagt cgcagagcaa cgatacccac accttaaata gtgcgagatt
     9601 acgacggttc aaggttgaaa acgacatatt tttcaacctt gaacctagtg aggggagtag
     9661 gtagaccgcc atagcggcta ccgtcctgtt tctagatact ttcagtatca gaaacaggtc
     9721 tcacatcaaa actgctccga gaatcgtaca tcaccttgat agaaaccacg gatgtcgttg
     9781 ataccgtaac ggagcatggc aatgcgttcc tgaccaagac cgaaggcaaa gccagagtac
     9841 ttggttgagt caataccact catttccaat acttgcgggt ggaccatgcc ggcaccgagg
     9901 atttcaatcc agcctgtttt cttacataca ttacagccgt caccaccgca cttgaagcag
     9961 gaaacatcca cctcaacaga aggctcagtg aatgggaagt atgatggacg caggcgaatc
    10021 tgacgctctt caccaaacat tttcttgata atcatttcaa gcgtaccttt gagatcgccc
    10081 atggatacat tttcaccgac aaccaagcct tcaatctggt ggaactggtg gctgtgggtc
    10141 gcatcatccg tatcacgacg gaaaacacgt cctggcgaaa tcatcttcaa agcacctttt
    10201 gagaaatcat gctggtccat ggtacgcgcc tgaacaggcg acgtatgggt cctcataagg
    10261 atttcttctg tgatgtagaa ggtatcttgc atatcacggg ctggatgatc tttcggcaaa
    10321 ttcatcctct caaagttgta gtagtctttt tccacttcaa atccgtcaac gatttggaag
    10381 cccatgccca agaaaatatc ttcaatttct tcagaagttt gggtcaagac atggcgtttt
    10441 ccgaccttaa cctgacgacc tggcaaggtc acatcaatgg tttcggaagc tagttgttgc
    10501 tgaatttttg ctgctgcaac cttttgagca gtttcttcaa aggcagttgt cagcacatca
    10561 cgcacttcat taacctgttt accaacgatt ggtttcatgt cgttagacag gtctttcaag
    10621 cctttcaata agtccgtcaa agaccctttt ttacccaaaa ccgcaacacg caggtcttgt
    10681 agttcttttt ctccttggtg ctggatttct tttaactttt ctagtgtcgt ttggcttaat
    10741 tcagccaact gttgttcaat gtttgacata attcctccaa taaaaaacgc atgacaccac
    10801 tcgtatcgga gcgttgtcac gcggtaccat ccgttttcat ctgcaaggca gaccttagtt
    10861 ctatgaacct atattcacaa gtgaagtgct tgcacataag agatggtttt ccgctaatca
    10921 aggctgtttt ttgagggaat actgactgta ttttgaaaaa aactaacgat gagtagctga
    10981 aaaactagcc ttagatggaa gtgttgaact gtgaatgcag gttctaagtc cttgtgtgag
    11041 tgaattcgac caactttcac tgattgagct tgcagtcacg gctcaactcc ctggacagct
    11101 tccataaggt gtactagtct cacagacttc tattcaatta aggatatttt atcaaaaaga
    11161 agaaagtttg gcaagaaatc ttcctagaaa actccctcta tctaggatag aaaggagttt
    11221 agttttatag ttttgcctgc catttattgg cccaaacatt ctcatttggc acaccaaaga
    11281 atgcgtcttt tccttctaat ttatcaagta aggattcgat agtattttta ttgtgatggt
    11341 aggcattgat agcagttttg accatggtaa catcgtgaag gtgagttgta tagtttagtg
    11401 aaatgaaaat ggttggaact tcatgtacat accaaggaac ttcatttgac atggccgttt
    11461 tccactggat gcggtaatta ttctgcgctc catatccaat gacattggca acagtcaagg
    11521 ctaaatcaac ttcttctcgg tattgcagcg tttttccttt gatccgagtc gtaccatcat
    11581 tgacggtcac ttcatagcca cgcgcttcca aggcagcttt aaggttatca accacttctg
    11641 tacttgccgc cataatccct tctttttcac cttctaggta atacaatcgg attctgcgat
    11701 gggtttctgg actgattgga agatttttct gtgtatcttt caacagcgta attgccttgt
    11761 ctgccgcctc acgttgccaa gccaagtgtt cttcacagcc gataatagcc aattcatcag
    11821 ctgattttag taaggttcct tccgcttgct tgatatgcag attgagtttg gctttcagac
    11881 ctaaaatgcg acggagagca tcatgaaggc gttcgtcagt aatcactccc ttgcggtagc
    11941 cattgagcat aaattcaaaa tcttcttcta agttattgaa gaataagaac atatcgcaac
    12001 ccgcagcaat ggctgcaggt acgtagtctt cacgacgcat ggctgctgtc atccccaaca
    12061 tgtggctggc atctgttatg accaagccat tgaattccag tttttctttc aataaatctt
    12121 ggatcaattc aggagccagc gttgcaggca aaatatcttc gtccgtcaaa cttggattga
    12181 gtcgttgttg gtaatatggc aaggctatat gtcccgccat aatcatctca acaccagctt
    12241 caatatgatg acgatagaca cgaccaaagc tcttgtccca ttcttctgga gagagctcat
    12301 tgacgcctag gactaggtgc tgatcacgtt cttctttacc atctcctggg aaatgcttga
    12361 tacattgaat aacatctctt tcagcagtca atccttctaa ataggcactg gtataggtaa
    12421 taacatcatc cgcatttgta ccgtaggctc tggtattgac gatagtattg cgccagttct
    12481 gaacaatgtc cacgcaagga tcaaaattga catttacacc taatgcagtc tcctcacgcg
    12541 cagatacgag acctgctagg tagggaatac gaggatcccg actggcttcc gattgagcac
    12601 ctgaggaaat ataggttcct cctttcacag ctccatctcc acctgagtca cagttcgctg
    12661 caaccaagag aggtactttt gaatagcttt gcagctcgtt aatcagtcct tgtacctgtt
    12721 ctggactgcc attcatataa cgggcaccac ctatgtggta tcgattcatc aattcctgat
    12781 tactcagatt atttccgcta aaagcgtctc ctccgaagaa gaatagatta acaaacaatt
    12841 gaccgatttt ttcttcatca gtcagatgag ccaaggtatt ttctacccag gtaatctgct
    12901 catcattcaa acaatagggt ttcttccgca aatcaaccaa tctaggcatt ctttcctcct
    12961 tattttccgc gccaaatctg tgtatctacc aagccacagt aggcatcaac aggcgatact
    13021 cctgtaaagg ctgactctcc tgctagttta gcaaccaagg cttctaatgt gacaggcaag
    13081 ccatcgtagg cattgatata ggttgcaacc tgtggcatat ctgcaagtgc aaatgggtgc
    13141 tgaacagaga caataatggt tggaatttcg tgaacataga aaggctggtc tggtgttccc
    13201 ttggcagctg gccaaataat tcgctgagtt gttcctcctg agttgacatc aactaaattg
    13261 ataatcaaat catagttatc tgtgatttcc gcaattggac gcttggaagc atagacattt
    13321 gcaatcgctg ctggtctttc ttcttctggc aatttggcaa tccgtgcctc cgtattttcc
    13381 cagatagata cttggtgacc tcgagcttct aatagttctt tcagcgtatc ggctgctctc
    13441 ttcttgccag cattgatcat ggcaccaaaa ccacccttat agccatctac ttcgaccaac
    13501 aaaatacgtt tgtagcgttc tggattgact gggaaaattc cttcttgttt ggcttttacc
    13561 aaggtaatcg ccttgtcagc tacttctttt gcaagctgtt tcgcctcatc tctaccaatc
    13621 aaagccagtg cttcttcttt tggcaacatg atttcctgac gacgaccttc aaacaagtga
    13681 agaccgagtt tagctttcaa gcccaaggta cgacgaaggg catcatggag gcgttcctca
    13741 gaaagcagac cattttccaa tccttctttc atccattgca aatcttcttc tggatcgttg
    13801 aagaaaagga aaagatcaca tcctgcctca atagctgtag gcaatagctc acgacgcggc
    13861 atagaggctg tcataccgat catgtgactg gcatccgtga caatggcacc attgtaacct
    13921 aactctccac gaagcaattc atccaagagg gttttattca gcgaagctgg caacatgtca
    13981 tccaattcac gttctggatg catttctttc tcaacatttg gcaaatgaat gtgccccgcc
    14041 ataatacctg ggagacctgc gtcagccatc tcaccataaa tccgtccaaa tgtttccatc
    14101 cattcagact tggtcatggg attcgaagcg aaagacaaat gatgatctcg ttcgtcaata
    14161 ccatctccag gaaaatgctt agcaaacggc acaatgccat gttccataat gcctcgcatg
    14221 tattctttgg acaattcaat gacttggtct acattagctc cccatgttcg gtttgcaatg
    14281 attggattgc gccaattacg gcttaggtcc ataatcggtg caaaggaggc attgcagcct
    14341 accgcagctg cttcaagtcc tgcaactttc cccatttcat aagcatagcg gggatcggtt
    14401 gtcgcagcaa tcttgatttc atccccaatt ttcgttccat ccgtaacagc gccatttcca
    14461 ccagattcgg tattggctgc aatcaagagg ggaatcttag acttggtctg caaaatatag
    14521 ttttgctccc aaatatctgc agcaggtcca ggattgtaac gaacagcagc aatcttataa
    14581 tcatttaata ctccagtcag atagtcttct gtccgctggc tccccatgtt gacaaagagg
    14641 tgaccgattt tttcatccaa ggtcatctga gcaatggttt cttcaatcca agaaatagcc
    14701 tcttcatcaa ggctgtaagg tttcttccgt aaatctacta aatgtgtcat gtttgcctcc
    14761 aaaaaagcta gagggcatag cgcctctagc ccttctctag tcgtctaaat tattccaaaa
    14821 tgcctgtatc aattctgttt ctttctcacc tgtgaatgct tgatgagcaa agtcatctgc
    14881 aatcgtttct gtcaaatctg cccaagattg catctctcgt cctactaaaa taggatagaa
    14941 atggacacca ttcaattccg ccgccttcaa atcaccaggc gagtcaccaa tcatcataat
    15001 cttattagga caataccctt cctctattaa cagtttgatg acatcttctt tcttgccacg
    15061 gtcttgacag tacaattctg taacaaagtc catcaaacct tggtctgtcc actcttcttc
    15121 tactgcttcc ttattagcag aagaaactac gtagactttt cctaactggc tcaatttttc
    15181 caagcccttg tgaacgccga tgaaggctaa aacaggacca ctataatgct taatggcttg
    15241 attgacttga gtggaccatt ttaaggctaa ttccaagtct tttgacggct gctcctctat
    15301 caatctttcc agagaagcat ttgacaaaga atctgtggtc gcgacccaat ttttcaagtt
    15361 gtctatacca gtcacacctg caaattctag gcctttaact aagccaacaa aacggttaat
    15421 gccacgttct cgtgagtaga gattaactcg attccattca gctaaaaacg gttctttatc
    15481 ttcaacacca aaaacttccg cagctaaggg gccgaagaaa agcttatgtt tgtaggtcat
    15541 ggtgtccatg gcgcagccat cagagtccac acaaaatacg tattccatct tcatcatcag
    15601 actcctgaat aggctgagaa gccaccgtca atcggtaaaa caacaccgtt gacaaaactt
    15661 gccaaattct catctgccaa gaagaagacg ccgccgacta gctcttccga ttcaccaaac
    15721 cgtcccattg gtgtattggt caaaatcttg ttggcacgtg cggtcggttg acctgactcg
    15781 tcaaaaagca aaccacggtt ttggttggtt accaaaaatc ccggagcaat ggcgttgcaa
    15841 cgaatactaa cttttgaaaa atgtactgcc aaccattggg taaaattact gatggcagcc
    15901 ttagcacctg aataggcagg aatttttgtc aaaggagtaa aggcattcat gctggagata
    15961 ttgataatat ttgctccgct acggccaatc atatcctgtg caaagacctg tgttggcaat
    16021 aaggttccta gataattcag attgaaaaca aagttaatcc ccgcctcatc taggtcaaag
    16081 aaggttttcg tatccgctgg aagacccact tcatggaact cattatccgt tgtcgctttc
    16141 ggattattgc cacctgctcc gttgattaaa atatctaccg gaccgaaatc tgccaatacc
    16201 tgttggcgaa cttcttccaa attttccttg gaaaggacat tagatttgta agccttggca
    16261 ctacctcctg ctgcaacaat ctcatcaaca aaaacttggg ctgctgcttg atttaagtct
    16321 aaaagagcca ccttggcacc agccttagca aattccttgg ctaaataacc acaaagtact
    16381 ccgcctgctc ctgtaatgac aacaaccttg tccttaaatt caatgacacg cgtcatagtt
    16441 atccctcctt tgttccaact gtttttgctt taataccaaa atctggtgtt ttgccagccg
    16501 ctcgcatatt ggcttcgtat agaccattaa agtaagtcgc cccgagcgct cggtcataca
    16561 gaccgtagcc cggtgtcttg gtctgatctc cccagatacg acgaccatga tctggacgca
    16621 aagaaccttg ccaatcatag tccaccaata atttaacaac agcattcata tcaatgtcac
    16681 cagcctgtga caaatgtgca gtttcttgga agccccaagc acctgccgtc acattgcgcg
    16741 tatgcataaa attgatacga ttgcgtttga gagcatattc cgtcatggca aggacatcat
    16801 ttttcggatc agaagcatag gatccgacac acatggtaat tccattgtgt tctgaatcat
    16861 agagattcaa aaatcgttca acggcttctt gaccagtaat aatacgtggc aatccgaaaa
    16921 ttccatacgg agggtcatca gggtgaatgg ccattttgac gccagcctcc tcagccgttg
    16981 gcagaatcgc tttgataaaa tactccaaat tcgcccataa atcttcttca gagatattct
    17041 gacgataatt ttcaataata gccttcattt cttccttgga ataggacgaa tcccaacctg
    17101 gtaagttcaa atcatccgcc acaggatcaa cacctgctaa atcagatttt aggaaagcta
    17161 aagaggttga accatccgga agtgggtggt gcaagtctga acgggtccag tcaaacacag
    17221 gcatgaaatt gtagcaaaca actggaatac ctgcagcccc gacattacgg atggaagtct
    17281 tatagttttc gattaaggca tcacgatttg gcttcccttg tttgatgtct tcatgaacag
    17341 gaatggattc aataacagta atttcaagtc ctgcctcttc taccattttt ttcagctcta
    17401 gaatgttttc aaggggccat gcctgaccaa ctggtacatc gtagactgcc gttacgattc
    17461 cctgcatacc agggattgct ttaatctctt ccagtgtcac aggatctttt ttcccgtacc
    17521 agcgaaatga cattttcatg atttactctc cttctattgt tcaaaaaact ctttggcatt
    17581 gtaataggat atggcttcta caaattgccc taaacgattg taatcttctg gcacttcttc
    17641 atcaacaatc cattgaccaa cataggtcgc taaaatgcgt ctgaaataat catgccgttg
    17701 ataagataaa aagctacgcg aatctgtcaa cataccaacg aaattggcaa gcatgccctg
    17761 ttctgcataa gcattcatct ggtcaatcat gcccaacttt gtatcattga accaccaacc
    17821 agcaccaaat tgtagctgac tacgaacccc ttcctcattg gactggaaat tcgccaaagt
    17881 attggccaga gcaatgttgt aacttggatt gagattgtac caaatcattt ttggaagggc
    17941 attttccttg accaagttat ctaagaaacg attcaaattc acagtcagac atgtctggtc
    18001 tccaatcgaa tccacaccta catctgctcc aagtttttca aacagtccgg tatggttatt
    18061 ccgtaaggca ccgaagtgaa cttgtgtcac aaagccatgt ttcttgtaca atcggcacaa
    18121 ttctctaaag atagcagtct gccatttatt gatactggat tgaccagctt ctttacctgc
    18181 aagtcttgct agtaaaattt catccaactc ttcgagggta gcctcttcat aggaaatagc
    18241 cgtaaaactg atgtcacttg ctcggcaacc gtgttgagca aaataggaaa cacgctgacc
    18301 aagtgcctct acaaaacttg caaagtctct tactgctacc cctgttacct cctccaagcg
    18361 tttaataaat ggattgaaat ttcgatgttc tataaaagct tcatctggtc ggaaagtcgg
    18421 tgccactaca gtatctatgg tcgagtcctc agccaagcgc ttatgccatt ccaagctatc
    18481 aagaggatgg tcagttgtcc caataaaggt caccctaaca tccgcaatca gcttccgtgg
    18541 actaacttga tgttcttgca aataagcatt gagctgatga tagagtcgct ctgcattttc
    18601 ttcggtcaac aattcctcaa caccgaaaac ctgtcgcaat tccaaatgac tccaatgata
    18661 gagaggattt ccgaaagccc gttccaaggt tcttgcccag gccttaaact tatctagttt
    18721 acttgctgaa cctgtgattt cttcttctga gataccatta gcccgcatca agcgccactt
    18781 ataatggtca ccccccagcc acaaatcaac aatatcttca taaaccttat cttcaaaaat
    18841 ttccttcgga tccagatgac aatggaaatc atagatagga agctctgcta ttttcccgta
    18901 aagctcctta gcaggctcgt tttttagcat aaagtttttg tcattaaaac tcatttcatc
    18961 cctccaaagc tgcaacaaaa ctgcgagcaa tcgtcgttac actttcatag ccttcagcct
    19021 gcactcgact tgccagagcc gaaccgactc caacagcaca agcacctgcc tttttccaat
    19081 ctgcaacatt ttcaaccgaa actccacctg aaggcatcaa attcacatca ggaacagggc
    19141 cgtgaatatc cttgataaat ccaggaccca agacaccacc agggaaaagc ttgataatag
    19201 gacatcccgc ctgactagct gtgacaatct ctgttgcagt tgcacaacct gggaagtaga
    19261 atacctcagc ctcttgcgcc aagtcttgaa tttctttatc aaaatgagga ctaaccagga
    19321 aactagcacc tgcatcaatg gccgcttgtg ccagcttaag attcatcact gttcccgccc
    19381 caatgactac ggatgaatca tctgagaact cctcttgtaa ttctttgata actgtcgctg
    19441 catttggagt cgaaaatgta atttcaatat ttcgaatacc tcccaaaatc gcatggcgag
    19501 cgatctcttt cgcatcttct tcatcttttc cgcgaatcac tgcgaaaaag taattttctt
    19561 ttaattgctt taacatcttg acctccaatt agaaagcgtt gtcatttata gctatagtat
    19621 acaattcatc agatgatttg tcaataaaaa agcaaaaaat acccagaaga attcttctgg
    19681 gctgtctatt ttttatttct atttatgatc gttgagacca tgcaaggtca tatctttatc
    19741 gaaccattcg cttaaaacaa cctgtcttac tgatataata tgtgctaaca tgctatcata
    19801 tgctgcaaca gggtgacgtt gcttgatagc cattaagata ttcctgtggg cctgtaaact
    19861 atccgatttc atctgacgat ttgaatagtt ttcattgatt agatgaatag attgattaat
    19921 gacaggcatg agacttgtca tagccagatt cccactcatc tcagccagca tacagtggaa
    19981 ttttacatcc aactccagat gcttcgtgtc tccagcagca acagcctctt caatagcaag
    20041 gacaatttct tctagtcgag caatgtcttc gtccgaggca aactgagcga cacgctccgc
    20101 aattctcggt tcaagtaaat accgcacttc aaacaaatct gtcgtcaact tcacacggtc
    20161 tcttaccaaa gaaaaaccta ggggatcctc tgatactcct tttttagagc tgatataggt
    20221 tcccgaccct tggcgcacct ccagaatatt tcgagtagcc aaacttcgca cagcttcacg
    20281 aacagtcgaa cgtccaactt ctaaatcttg ggccaattca tactcatttg gaagcttatc
    20341 accaacttca tatcctcttt ccaaaatcaa ttccaataag cgctctgatg ctttttctac
    20401 caagggtttc gccatgtgct tggtcctcca atacaacttt ctatttgcta ctattatagc
    20461 tcatttcctc ttataatggt agtttggaat ggcagtccaa cgtttcttga tttctcttac
    20521 aatttgcttg ggttgtctat ttcttgagaa taatcctttg tgatttcctt gaatgcgaat
    20581 gataccgact gtagtttcaa aatcagcaaa attccatacc tgctcaccaa ctaaattcgg
    20641 aattgaatca aagacgcaat ggttcatatc gtagtaatcc acctgaaatt cctctgtata
    20701 aggaatatcc cacatcgaat gcaaacctgg aagagtatct gcaccatatt ccgtaatcaa
    20761 aatcggcttg tcaggataaa gtttttgcca agtctccaac tcttgacgga gccctttctc
    20821 tgccgccttc aagtcaccat gagcaacata ccaaccatag taacgattga gacagacaac
    20881 atcgaccaaa tccataacct catccttatc aggcgttgct gtcataatat tgaccaaggt
    20941 cacaggacgc ttagacggat ctctgtccct catgcgctga atcagaggtt caaagtaagc
    21001 acgtgctccc ttttcatgcc ctgccggttc attggcaaca acccacatca ctacagaagg
    21061 atgattctta tcacgcgcta tcaattcatc aatcaccagc tcatgagcct ccttggtttg
    21121 cataacttcc caagtatttt ttgcttcttc agaatcccct gtcaaattca aggctgcatt
    21181 gaaaagctgg aacaggccaa ctgctggcac ttcatcaagg acgacaattc ccaaacgatc
    21241 tgccagccgc atcatttcct cagaataggg ataatgagat gtcctaaagg aatttgctcc
    21301 aatatcttta agcaaattca aatccatcag attggctgct tcattgaaac cacgcccatt
    21361 gatgaaggta tcttcatgtt taccaaatcc cttgaaatag aaaggttttc cattgatgaa
    21421 aaactgccca ttttcaaccg caacactacg aatgccgaat tgttctgagt aggtatcgag
    21481 taagttttct ccgtcaacta attcaacttt agcagtatag agataggcat ccaagacctc
    21541 ccaaaggcgt ggatgttcaa tgaccatttg tccattttca agaagaccta cttcctgtcc
    21601 ctcttgatct aaaatagaca tacggatgga atcgtactgc ccaactgtct tcacatcaac
    21661 attcactacc gcctgagtca aatcttgatt tagctccgtc cgaataacaa tatcctcaat
    21721 cctattttgg ggacgtttat agagatgaac tgtccgctgg ataccagcat agttgaaaaa
    21781 gtcaaaattt tccttgacgg tcttcacaat ttgaccattc tcatccactt cttcgctata
    21841 attccctact gggagggttg tgtaattcaa ctcattatta gcacaaaccg tcaaacggaa
    21901 ttgatcagcg tcgtataagt ttgacggaat caagcattca aaaggtgtga aaccaccctt
    21961 gtgctccctg attaactgac catctgcata gactttagcc tgatgtgtca ccgaaccaaa
    22021 gcgtacaact aactcttcat catcagctac tactggaaga gctaccatca gttcatacca
    22081 attatctccg acaaaacgac gtttgtcctt atcaacaaca acatcattaa aagaactcgg
    22141 tacaaccatt acctcatctg tatctaacag agtatgaaca tcattgttcc cttgcttaaa
    22201 tttccatatt ccatttaaag aataaactga gcgtgttttt gtctgtattg gatacaacat
    22261 actttttcct tctttctatc gttttatttc acgtgaccct tttttcaata gcaaagcaat
    22321 atctgacgga ctgatgacat tgacatcacc ttcaatggta tgtttgaatt gaaaactagc
    22381 catccccaag tcgactattt cctgtggcgt tttttcctgc aaatagccca aaataattcc
    22441 tgctgtaaag gcatcccctg tcccaactcg gtccaaaact tgaatggatt ctttttccgt
    22501 ttcataaaga ataccatctt gatacaaata tgctttcagc aaatattcat tattgtaggt
    22561 catttccctc tgggtaaaag caagactttg caaagaatac gtctccgcca gacctttaac
    22621 caccgccaac aagacttctt tattttcata agggcgcttc aaccccaatt catccttcac
    22681 atcttttcca gattcattga agagttgaat aggctctaga ccaaagcaaa tatctgctaa
    22741 acctacaaaa ggagataatt tctccctcgc ttcttcaaaa gaagaccaca aactttctcg
    22801 ataattcaag tcaaaagaaa ccttaacacc ctgcctcttt gccatttcca tcaaacgaaa
    22861 ggccagtcga taaatatctg gattcaaagc gaccgttata ccactgacat gaaaccaatc
    22921 gacaccgtca aacatacctt ccaaatcata ctcttcttcc ttggataagc taaaggcaga
    22981 ataatcacgg tcgtaggtca cctggctagg tctaagagag aagccctttt gataataata
    23041 aatccccagc cttccttttt ttcgaatcag atgcttctgt ccaatctgcc gtgcaaagag
    23101 aaagtgttct gccatctttc ccacttcatt ttcaggaagt gccgtcacca actcaactga
    23161 atgccccaac tgtgctaaac tggccaagac attcaactct gagccaccaa actgacaatc
    23221 taaactggta gcttgtgtta gggtctgata atgaggagga gatagccgca ataaaacttc
    23281 tccaaacgct aagatttttt tcataccgcc tcctagatga aagacccagc tggaatcctc
    23341 caactaggtc gttattgtat tatttatcgt cgtattgatc aaccttatat tgagtaacat
    23401 ctacataatc catatcagat aatggaacat ttttcgcaat cgcacgcaca cgttcctcat
    23461 cttttgccgc tttcataaca tggattttct cttgaatacg tgccatttct tcacgatcta
    23521 gtttgtaaaa tttcatcaag aacaaggata cgctcaatac cactgctgga atcactgtca
    23581 ataggacaat caaagccatt ctcaaatctg ttgagaatgc tgtttcaacg gttgggtact
    23641 ctttaccaaa accaatacct gctaatacaa atccaacaac cattggggca aatgaagaag
    23701 caattgagtc tgtcaatgag aagatagtcc caatcatacc tgacaagtaa cgaccagata
    23761 ctgaagtttc atagtctgta atatctgccc ccattgtcaa taccaaacca gatggagcac
    23821 tagatgcgta gcgagcaatg atataaacaa caaagaagcc gattgtgtag agattccaat
    23881 ttgtcaaatc caacatacct ggtttagctg aaaagagcat cgctgccaag gtcacaagac
    23941 cagcaatccc aagttgaagc gctgtaatat aggcaaagcg taaaccttta cgacgagcca
    24001 attgcgcagc accaactgtt acaagaatac ctggcaaaat caacagcata gacattttac
    24061 cagaaagagc atagtcaccg aagataatac catacagcat aaccctgata acggaatctc
    24121 caaacagttg agcaacaaat tttacaaaag ctgcagagat tgaaagaacc tgcaaaggtt
    24181 tgtttccttt gataactttc cagtagtctt tcaacttagt ttcttgtgtc ttctcaccaa
    24241 gaccaaagta tttcttattg tctttctccc aaataccgat aacagcaaga atacccagaa
    24301 cagctgaaat tactactaca ccgaggaaca attcttggaa gaaacttcga gtgaaaccac
    24361 catgttttgg aaccaatgtc cctgacacaa caacctgact accagtcatc aacacagttg
    24421 tcgcaatacc atcaacaata ttaaagattg gacgttgctt tggatcattg gtaagcgcaa
    24481 cttgtcctgc ttttgtaatc gtctgttgaa gtgaatatcc aattttatgg ataatcaaaa
    24541 caaggatgaa tagaggaaga cggagactct cgctaactcc acttaaacct aacatcataa
    24601 aaagagaagc tgctgtaatg atattcccca aaaccaaaat aggacgatat ttaccaaact
    24661 tagtatccgt tttatcaatg ataactccga ttgtaggatc aatgaaacca tcaaagatac
    24721 gaatataccc catcagttga ctaacaaaca gggctgcaag accaagcaca cctgtcgata
    24781 aataagtcac gaaagtaaaa gcaaccaagt aaatattcgt cgatgaattg ttcatcgcaa
    24841 ataatatgat ttgccataac ttggctttgt tataagtaac gtcttcgaca cgttgctcaa
    24901 catgtttttc cattggaaat tcctcctaaa tg
//
LOCUS       DBNFCK010000016        24643 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.016, whole genome shotgun sequence.
ACCESSION   DBNFCK010000016 DBNFCK010000000
VERSION     DBNFCK010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 24643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24643
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.016"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            64..1377
                     /locus_tag="AC1PIW_000523"
     CDS             64..1377
                     /locus_tag="AC1PIW_000523"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIP6605487.1"
                     /translation="MIEKVFRDPVHNYVHVDHELIYKLINTKEFQRLRRIKQLGTTSY
                     TFHGGEHSRFSHCLGAYEIARRITQKFEDKYPQIWDTNESLLTMVAALLHDVGHGAYS
                     HTFERLFDTDHEEMTCAIITSPETEINALLKQVSPEFPDKVASVIKHTYPNKQVVQLI
                     SSQIDVDRMDYLLRDSYFTGANYGEFDLTRILRVIRPTENGIAFKESGMHAVEDYVLS
                     RYQMYMQVYFHPASRSMEVLLQNLLNRAKLLYTGEQEFFARTSPRLLPFFEHRIRLTD
                     YLSLDDGVMNTYFQSWIDGPDRILSDLAQRYINRKVLKSITFKAEEEEALDRLRSLVA
                     DVGFDPEYYTAIHHNFDLPYDIYRPNAEKKRTQIEIYRKDETLVELSSLSPIVHSLSG
                     TIHGDSRFYFPKEMLEETGIFAPQIADFNSHIHNDHFIIGESNEH"
     gene            1367..2176
                     /gene="yidA"
                     /locus_tag="AC1PIW_000524"
     CDS             1367..2176
                     /gene="yidA"
                     /locus_tag="AC1PIW_000524"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HIP6605488.1"
                     /translation="MSIKLVAIDIDGTLLNSQHQITAEVHSTIQEAKKAGVKIVIATG
                     RPISGVQAILEELNLTEAGDYVITFNGGLVQDAATGEDIVKDTLTYEDYLDIESAARK
                     LKLPMHASTKEGIYTANRNIGKYTIYESTLVSAPIFYRTPEEMADKEIIKTMMVDEPE
                     ILDAAIPLLPTILTEKYNVAKSAPFYLEITPKTVNKGQAIIHLAEKLGLTMDQTMAIG
                     DQENDRPMLEVVGNPVVMENGNPELKKIAKYITKSNDESGVAHAFREWVLK"
     gene            complement(2229..2981)
                     /gene="tpiA"
                     /locus_tag="AC1PIW_000525"
     CDS             complement(2229..2981)
                     /gene="tpiA"
                     /locus_tag="AC1PIW_000525"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIP6605489.1"
                     /translation="MSRKPIIAGNWKMNKNPQEAQAFVEAIAGKLPAGDKIEAAIAAP
                     AVDLNALLWFAKDSELKVAAQNCYFEDAGAFTGETSPKVLAEMGVNYVVIGHSERRDY
                     FHETDEDINKKAHAIFRNGLTPIICCGESLETYEAGKAVEFVGAQVSAALKDLTADQV
                     ASLVIAYEPIWAIGTGKSATKDDAQNMCKAVRDVVAADFGQEVADKVRVQYGGSVNPS
                     NVAEYMACPDVDGALVGGASLEAESFLALLNF"
     gene            complement(3156..4352)
                     /gene="tuf"
                     /locus_tag="AC1PIW_000526"
     CDS             complement(3156..4352)
                     /gene="tuf"
                     /locus_tag="AC1PIW_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HIP6605490.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGTVRVNDEIEIVGLQEEKSKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVISKPGSINPHTKFKGEVYILTKEEGGRHTPFFDNYRPQ
                     FYFRTTDVTGSIKLPEGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(4708..5679)
                     /locus_tag="AC1PIW_000527"
     CDS             complement(4708..5679)
                     /locus_tag="AC1PIW_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti sigma factor C-terminal domain-containing
                     protein"
                     /protein_id="HIP6605491.1"
                     /translation="MTMKTFEEVTKKSKRKNLWKTVGLSSFIGFILLLIGIQTVKIIV
                     GAREGSENWKQIILMEISYPNIKHDGIKIQSTSYVSGKLYCNLYKDLAGVPMAFGQHE
                     IYYDLFDRHYDFAQHIPGNTFKYLYDFTTNTKIPVFYNRKKSVWSVQDIANELHYLKD
                     MSGQLVEVAISFDKKYSLAEIKQMIPSNLKQNWYWIGTEGKQNTHYYRPEQLLGLDPE
                     DIQLVFSDMPGDYMTGMDFMKEFLESGDQKRAQYLDIPVDDLQAFVDKFGDTDFTKQE
                     EIDKLEFAGIILTGKAEDFAQLEGKDWIYSSSIGASIPIQPYYQLHH"
     gene            complement(5660..6163)
                     /locus_tag="AC1PIW_000528"
     CDS             complement(5660..6163)
                     /locus_tag="AC1PIW_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIP6605492.1"
                     /translation="MSIKLDQYEKEVIGIAEEISYYLQKSGANYADSQDIAQDILVKI
                     LEAELVLPFEKLRAWLYRSAVRAYIDKYRRDKRYHEILQKEFFTPENFLVYDQEDYEE
                     LYQAVADLPEKYQQVIDLYYFQDMSIKEIAHILGAGQSWVKISLHRGRKQLRKILEER
                     GYDYEDF"
     gene            complement(6278..8974)
                     /gene="ppc"
                     /locus_tag="AC1PIW_000529"
     CDS             complement(6278..8974)
                     /gene="ppc"
                     /locus_tag="AC1PIW_000529"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HIP6605493.1"
                     /translation="MAIQKLENYNNKEAIREEVTILTSILEEVAAQMMPAETFAKIVE
                     LSQLSRQDDYQALIAIISQLNNDELAVISRYFAVLPLLINISEDVDLAYEINHQNNID
                     QDYLGKISTTIDMVSKQENAAEILEKLNVVPVLTAHPTQVQRQSMLDLTKHIHELLRR
                     YRDVKLGLLNKNKWEDELRCYIEIIMQTDMIREKKLKVTNEITNVMEYYNSSFIKAVT
                     KLQREYKRLAAEKGIVLNNPRPITMGMWIGGDRDGNPFVTAETLKLSALTQCEVIMNY
                     YDEQLYKLYRNFSLSTSIVNVSTAVKMLADLSEDSSVYRENEPYRRAFHYIQMKLANT
                     RDYLVHNKPSDVRYSNVAEFKADLLAIKQSLVENKSMALLKGDFTELLEAVEAFGFYL
                     ASIDMRQDSSIHEASVAELLASARIVEDYSSLSEEAKCHVLLKQLETDPRILSATHMP
                     KSEQLEKELAIFAAARELKDKLGEEIIKQHIISHSESVSDLLELAVLLKEVGLVDVDK
                     ARVQIVPLFETIEDLDNAPDTMRQYLQLDLAKRWIAGNKYYQEIMLGYSDSNKDGGYL
                     SSGWTLYKAQNELTQIGQDYGVNITFFHGRGGTVGRGGGPSYDAITSQPFGSIKDRLR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSATLDRMVTQMITDPNEIDGYRETMDEIVSDS
                     YRIYRDLVFNNPHFYDYFFAASPIREVSSLNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QNRVMLPGWYGVGSSFKRFIDKHPDNLSKLQKMYESWPFFRSLLSNVDMVLSKSNMNI
                     AFEYAKMCESEEVRNIFHVILDEWQLTKEIILSIEQNDELLAELPFLKASLDYRMPYF
                     NVLNYIQIELIHRLRRGELSKEQENLIHITINGVATGLRNSG"
     gene            complement(8995..10209)
                     /gene="ftsW"
                     /locus_tag="AC1PIW_000530"
     CDS             complement(8995..10209)
                     /gene="ftsW"
                     /locus_tag="AC1PIW_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division peptidoglycan polymerase FtsW"
                     /protein_id="HIP6605494.1"
                     /translation="MKIDKRQLLNYSILIPYLILSVIGLVMVYSTTSATQIINGGNPF
                     RTVINQAGFWIVSLVAIYTIYRMKLSFLRKKAVIYSVILVEVFLLAISRLFPPINGAH
                     GWIPLPIAGTLQPAEYLKLIIIWYLAHEFAKQQADIRTYDYVSLIKGSWIPKEFTDWR
                     VVSLLLLGLVATLPDLGNATIIVLIMVVMISVSGIAYRWFSTAVLGIVAASTVILGTI
                     RILGVETVEKIPLFGYIARRFAAYFDPFGNATNSGLQLTHSYYAMSNGGWLGRGLGNS
                     IEKKGYLPEAHTDFAFSIVIEELGFVGASLILALLFFLIIRIIIVGVRARSPFNAMMA
                     LGMAGMLLIQTFVNIGGISGIIPATGVTFPFLSQGGSSLLIISIGIGFVLNIDASEKR
                     RLIEEEIERTLI"
     gene            complement(10346..10711)
                     /locus_tag="AC1PIW_000531"
     CDS             complement(10346..10711)
                     /locus_tag="AC1PIW_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HIP6605495.1"
                     /translation="MKKISIMLMVVFSLFLAACTNTTEKQNNSAIEVTLQITTKDKEN
                     RQEVTAEEGDSVMDVLKEHHDIVEDNGLITEIDGVSQDTSTNTYWMYKVNGQMADKGA
                     KDLTVANGDEIEFYLETFE"
     gene            complement(10708..11043)
                     /locus_tag="AC1PIW_000532"
     CDS             complement(10708..11043)
                     /locus_tag="AC1PIW_000532"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase YidC"
                     /protein_id="HIP6605496.1"
                     /translation="MILVMVGSGLLFGFGTVVLWQVLSFAFIQFIWWLAIGPFIKKEN
                     IPLWLQSLLAGILVFLYGLTISFLSATQFGTSPIVFWLNGLVFDSLHATSTVLFYPII
                     DYIFRRFYK"
     gene            11240..12094
                     /locus_tag="AC1PIW_000533"
     CDS             11240..12094
                     /locus_tag="AC1PIW_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HIP6605497.1"
                     /translation="MRKKLNPMIVVGFFLSFFALIFITGVTGNTVNKTEVANEPAVTQ
                     SNTSTSSKTTNSSSSYYIANALEMKPIIDVSAWQRPSEIDYDTLSKNISGAIVRIQSG
                     SHTKNENTATDKNGLDKSFDTHIKEFQARNIPVAVYAYVTGNSIENMREEARSFYKAA
                     NKYKPTFYWLDVEEYTMDDMNAGVEAFRKELETLGAKNIGIYVGTYFMEDHSINVDKF
                     DAIWIPTYGDDSGYYNAAPNTDLNYDLHQYTSRGYVNGFEHHLDLNIITTLKDPNEVY
                     QKLFTTPE"
     regulatory      12118..12213
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            12272..12820
                     /gene="thiT"
                     /locus_tag="AC1PIW_000534"
     CDS             12272..12820
                     /gene="thiT"
                     /locus_tag="AC1PIW_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HIP6605498.1"
                     /translation="MPTSKLPILAEVAIFSAIALVFDKIPLFTMPQGGSVSLVMLPIL
                     LLALRHGLGVGVLTGGIVGTIQLFYGGYFLNVFQVFLDYAFSYAGIGLAGLVAPTLSK
                     QNNLKNATLIITLASFLGGSIRLLATFLSGIIFYADYAPDGMPVWFYSFTYNISYILP
                     STAIASILLILLYRARPGFYNL"
     gene            12923..13765
                     /locus_tag="AC1PIW_000535"
     CDS             12923..13765
                     /locus_tag="AC1PIW_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HIP6605499.1"
                     /translation="MASWISRIIKGMIIALGFILPGVSGGVLAAILGIYERLIGFLAN
                     IRKDFKENFLYFVPVGIGGILGIALFSFPVEFLLQHFQVPVLWAFAGAIVGTIPSLLK
                     ESTQKSKRDSIDLAWLIGTFIISGLLLYNLSDLVGTLPATFASFVLAGALIALGVLVP
                     GLSPSNLLLILGIYTPMLNGFKSLDLFGTFLPIAIGGVLAMVAFSKAMDHALKVYHSR
                     VYHFIIGIVSSSTLLILIPQASNKESISYAGANFVTWIAAIVLFILGAWLGLWMSKLE
                     EKYK"
     gene            13765..14247
                     /locus_tag="AC1PIW_000536"
     CDS             13765..14247
                     /locus_tag="AC1PIW_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HIP6605500.1"
                     /translation="MAKKVKIKKTLVDQILDKAKINHDSLVLNAFEGQLPPGIEEHEI
                     YKTLALTGDKTGPIIGIIPITEHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNFPIYIDQSALELGHLIVSAGEIGRSIRIDSQVLADFVKADFADLIEGRD
                     "
     gene            14250..14876
                     /locus_tag="AC1PIW_000537"
     CDS             14250..14876
                     /locus_tag="AC1PIW_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIP6605501.1"
                     /translation="MKIYFVRHGKTEWNLEGRFQGYSGDSALLPESYQDLEKLGKYLA
                     EIPFDAIYSSDLQRAHSTAVEIAKANHHCQTVLTTQQLREWNFGTLEGSKMAIFRAIY
                     PKQAWALKHNLALFNNDLFEAESVRQVTQRMVDFVQSLKGQDMETVLIVSHGAFLTAS
                     IHRLLGFPPAQLRHRGGLDNASVTILETSDFENFTELAWNDTSYKAKQ"
     gene            14979..16469
                     /gene="lysS"
                     /locus_tag="AC1PIW_000538"
     CDS             14979..16469
                     /gene="lysS"
                     /locus_tag="AC1PIW_000538"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HIP6605502.1"
                     /translation="MSTEHFEELNDQQIVRREKMTALAEQGIDPFGKRFERSANSAEL
                     KAQYEDKSKEDLEELGQTAIIAGRIMTKRGKGKAGFAHIQDREGQIQIYVRKDDVSEE
                     NYEIFKKADLGDFIGVEGDVFKTNVGELSIHARKLTHLSKALRPLPEKFHGLTDIETR
                     YRKRYLDLITNRESFDRFVTRSKIISEIRRYLDGLGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADYQDIMDLTEGIIQHTAKAVVGDGPVTYQGTEIAIHEPFKRIHMVDAIKEQTGVD
                     FWQEMSFEEAKALAAEHKVPVEKHHTEVGQIINSFFEEYVEATLIQPTFVYGHPVAVS
                     PLAKKNDEDPRFTDRFELFIMTKEYGNAFTELNDPIDQLERFEAQAKAKELGDDEATG
                     VDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(17057..18121)
                     /locus_tag="AC1PIW_000539"
     CDS             complement(17057..18121)
                     /locus_tag="AC1PIW_000539"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIP6605503.1"
                     /translation="MVELGISTFGETTPLEKTGETYSHDERIRQLVKEIELADAVGLD
                     VYGIGEHHREDFAVSAPEIVLAAGAVNTKHIKLTSAVSILSSMDPVRLYQQYTTIDAL
                     SNGRAEIMAGRGSFTESFPLFGYDLHDYEELFDEKLDMLLEIGKETNLKWDGHFTQSV
                     DNKPVYPRPVQEDFPIWVATGGNVESTIKIAKKGLPIVYAVIGAGAHRFKPLVDAYRK
                     VARNSGHDPKKTKVAAHSWGWIADDHDKAVEEYYHPTKVITDNIAKDRPHWSEMTKAQ
                     YLYSLTDEGATIVGDPKRVAEKIIKTIETLDLDRFFLHLPIGSMPHEDVLRAIELYGK
                     EVVPIVRDYFAKKEENQSQD"
     gene            complement(18247..19494)
                     /locus_tag="AC1PIW_000540"
     CDS             complement(18247..19494)
                     /locus_tag="AC1PIW_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6605504.1"
                     /translation="MENWKFALKSIMAHKMRSLLTMLGIIIGVAAVVIIVAMGTGVAK
                     SIEKSLAGDQNNVQVYFTAFSSSGTAFGPFGTASTEEPILEEEPDLTDSTLKGLLEID
                     GVSNYYSSISNIAEVRSGNKKAENVTITGVSQNFFAVKEYEILAGRQFTANDYSRFSR
                     MIMLDTALAEKLFGSIENSLNQTISVNSNAYLVIGVYKDPKAGTAIYGMNSGGNAVMT
                     NTQLAAETGMKENSQIFVHVEDVTRATEVGKAAADYLTKATGLKEARYEIYDMSAMLE
                     EFKSQMSGVTLFIGAVAGISLLVGGIGVMNIMLVSVTERTREIGLRKALGATRGNILI
                     QFLIEAMVLTTLGGAIGLAIAQTIVFLLNVSKALGERIAAEISIPVVLGSLAFSAVVG
                     IVFGVLPANKASKLDPIEALRYE"
     gene            complement(19495..20190)
                     /locus_tag="AC1PIW_000541"
     CDS             complement(19495..20190)
                     /locus_tag="AC1PIW_000541"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6605505.1"
                     /translation="MKNQLIRLTSINKSYKNGDQELRVLKNIDLEVEEGEFLAIMGPS
                     GSGKSTLMNIIGLLDRFSSGNYWLEGEEVSQLSEKKLAQVRNDQIGFVFQQFFLLSKL
                     NALQNVELPLIYAGVPSSKRKELAKQYLEKVELAERMTHLPSELSGGQKQRVAIARAL
                     VNTPAIILADEPTGALDTKTGEQIMQLLTELNNEGKTIIMVTHEPEIAAYAKRKIVLR
                     DGVITEDSRREGV"
     gene            complement(20171..21328)
                     /locus_tag="AC1PIW_000542"
     CDS             complement(20171..21328)
                     /locus_tag="AC1PIW_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIP6605506.1"
                     /translation="MLKTKKAKIALFGGLGVVAVALVGGALIFSNMLTSPSSSEDVAM
                     SMTYTVAKEGSIASSTLLTGTITAADEQYVYYDPSKGDLSEVLVAPGAKVEVGTPLIR
                     YDATELQSALDTAVRGRDKIGRQIADLRTNGQTVQTTGDAETDAAATATAQRSVDMQL
                     ADLNDSYADAQAAVDKAYTALTEATVTSTVAGTVVEVNKAVSKNSTSSQTVVHIVNQG
                     SLQVTGSLTEYDLANIAVDQEVKLTSKVYPDKTWTGKITYISNYPSSNQAGAATTGTS
                     GSGAKYPFKAALTSELGELKQGFSVNIEVVNTSKNILIPVSAVVPEEDKNYVWTLVDG
                     KAKKVEVTLGNADALNQEVTAGIAAGDKVITIPTPNLEDGKEVEANEEPAY"
     gene            21542..22891
                     /gene="gorA"
                     /locus_tag="AC1PIW_000543"
     CDS             21542..22891
                     /gene="gorA"
                     /locus_tag="AC1PIW_000543"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HIP6605507.1"
                     /translation="MKVFDIIAIGGGSGGIATMNRAAMYGAKAAVIEGSYLGGTCVNL
                     GCVPKKIMWYGSQVAEAIQHYGPEYGFTSQEVTFDFATLRKNREAYIERSRASYGNTF
                     NNNEVEVIQGFARFVDNQTVEVNGELIRAKHIVITTGAKPVVPAIPGSELGIVSDDVF
                     AWEELPSSVAVIGAGYIAVEMAGLLHGLGVQTDLFVRGNRPLRSFDTYIVDALMEEMQ
                     RTNLPLHTGKTPVSLEKTDDGQIQINFADGSNHIAQKVLWAIGRKPNIDKLNLKATSV
                     QLTSSGHISVNEYQETAVPGIYALGDVTGEKELTPVAIKAGRLLAERLFNNKPTAKMD
                     YTTIPTVVFSHPAIGTVGLTEDEAITQYGQDNVKVYTSAFTSMYTALENNRQMAKFKL
                     VTVGENEKVVGLHGIGYGVDEMIQGFSVAIKMGATKEEFDAVVAIHPTGSEEFVTMR"
     gene            complement(22941..23678)
                     /locus_tag="AC1PIW_000544"
     CDS             complement(22941..23678)
                     /locus_tag="AC1PIW_000544"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIP6605508.1"
                     /translation="MTELLDIYRTLKSKTWVDLTHQINEKSPHFPALPALEKKALFTH
                     KDGFFVEQFTVVGQYGTHIDPPIHFVEGARYLDEIDLKDLLLPLYVIDKSAAVIENND
                     YEITKQDILDFEAEYGPIAPKSFVAFRSDLSKRWPSQDAIRNLDEDGVQRTPGWSHEA
                     LEYLIEERQVKAVGHETLDTDSGVSAAKHGGSLSEEYYLLSKDIYQLEVLANLDQVPP
                     TGALISIAFPHWEKASGSPVRAIAILP"
     gene            23883..24422
                     /locus_tag="AC1PIW_000545"
     CDS             23883..24422
                     /locus_tag="AC1PIW_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HIP6605509.1"
                     /translation="MKTTSIKSLVYIAIGTALISTLSQISLSIGPVPFTLQTLAIGLV
                     ACLYKPKEAITSVSLYLVLGAIGLPVFAGFSGGFAALTGPTSGFLWGFLLYTIITSAL
                     TKSDSSPVTIFLACLLGTSICFLLGCCVFKLVSGASWTDTIGWTVLPFILPDLAKITL
                     VMICHRLLQPVIKKEAYFA"
     gene            complement(24434..>24643)
                     /locus_tag="AC1PIW_000546"
     CDS             complement(24434..>24643)
                     /locus_tag="AC1PIW_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018167072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HIP6605510.1"
                     /translation="EFTFEIEEKLLVLSENDKGWTKELNRVSFNGAPAKYDIRTWSPD
                     HTKMGKGITLSNEEFQVLLDAFANK"
BASE COUNT         7254 a         5428 c         4635 g         7326 t
ORIGIN      
        1 agcaatatcc ttgtgtttta gaaaaatcag gaatttatct cccttttttg ttataatgaa
       61 actatgatag aaaaagtatt tcgcgatcca gttcataact atgtacatgt tgatcatgag
      121 ctgatttata aacttatcaa tacaaaagaa tttcagcgac tacgccgcat caagcaacta
      181 gggactactt cctatacctt ccatggtggc gagcatagcc gcttttcgca ctgccttggc
      241 gcctacgaaa tcgccagacg gattactcaa aaatttgaag acaagtatcc acaaatctgg
      301 gatacaaatg agtccctact gaccatggtt gctgcgctac tccatgatgt cggacacgga
      361 gcctattctc ataccttcga gcgtctattt gatacagacc atgaggaaat gacctgtgct
      421 attattacta gtccagaaac ggaaatcaat gctctgttga aacaagtttc acctgagttt
      481 ccagacaagg ttgctagtgt tatcaaacac acctatccaa ataaacaggt cgttcaactc
      541 atttcgagcc agattgatgt tgaccggatg gattatctat tacgagattc ctacttcaca
      601 ggtgcgaact atggtgaatt tgacctaact aggatattac gtgtcattcg cccgacagaa
      661 aatggaattg ccttcaaaga atctggtatg catgcagtag aagactacgt tctcagccgc
      721 taccagatgt acatgcaagt ctattttcat ccagcaagcc gctctatgga agtcctattg
      781 cagaatttac tcaatcgtgc caaactacta tatactggtg aacaggagtt ttttgcacga
      841 acttcacctc ggctattacc tttttttgag catcgtatcc gtctaaccga ctacctcagt
      901 ctggatgatg gagttatgaa tacttacttt caatcatgga tagatggacc agatcgtata
      961 ttatctgact tagcccagcg ctatatcaat aggaaagtgc tcaaatccat cactttcaag
     1021 gccgaagaag aggaagcttt ggaccgcctg cgtagcctgg tcgcagacgt cgggtttgac
     1081 ccagaatact atactgctat tcaccacaac tttgatttgc cgtatgacat ttaccgaccg
     1141 aatgcagaaa agaaacggac tcaaattgag atttatcgca aagatgaaac cttggtagaa
     1201 ctatcctctc tatcaccaat tgtccattcc ttatcaggca caatccatgg cgatagccgc
     1261 ttctatttcc caaaagaaat gttagaagaa acaggaattt tcgcacctca aatagctgat
     1321 tttaacagcc acatacataa cgatcatttt ataattggag aatcaaatga gcattaaact
     1381 tgtcgccatc gatattgatg gtacactact aaatagtcaa caccaaatta cagctgaagt
     1441 acactcaaca atccaagaag caaaaaaagc aggcgttaaa attgttattg ctactggtcg
     1501 ccccatttcc ggtgttcaag caattttaga agaactcaat cttacagaag caggtgacta
     1561 cgtcatcact tttaacggtg gcttagtcca agatgcagca actggtgagg atattgtcaa
     1621 agataccttg acttatgagg attatctcga catcgaatct gctgcccgta aactcaagct
     1681 cccaatgcat gccagcacca aggaaggtat ctatactgcc aaccgtaaca tcggtaaata
     1741 caccatttac gaatccacac tagtaagtgc acccattttc tatcgcactc ctgaagaaat
     1801 ggctgacaag gaaatcatca aaacaatgat ggttgatgag ccagaaattc ttgatgctgc
     1861 tattccactt ctaccgacca tcttgactga gaagtacaac gtagcaaaat cagcaccatt
     1921 ttatttggaa atcaccccta aaactgttaa caagggacaa gctatcattc atctagctga
     1981 gaagcttgga ctaactatgg atcaaaccat ggccatcggt gaccaagaaa atgaccgtcc
     2041 aatgttggaa gttgttggta atcctgttgt catggaaaac ggaaatcctg aattgaagaa
     2101 aatcgccaaa tacattacga aatcaaatga tgagagcggt gtagcccacg cttttagaga
     2161 atgggtactg aagtagcctc ttaaaatatc aaaaaagcat cgaattcaaa atccgatgct
     2221 ttttgttatt agaagttcaa caaagccaag aagctttctg cttcaagtga tgcaccacca
     2281 acaagcgcac cgtcaacgtc tggacatgcc atgtattcag caacgtttga tgggtttaca
     2341 gaaccaccgt attgaacgcg aactttgtct gcaacttctt gaccgaaatc agcagcaaca
     2401 acgtcacgaa cagctttaca catgttttgt gcatcatctt tagtagctga tttaccagta
     2461 ccgattgccc agattggctc ataagcaata acaagtgatg ctacttgatc agcagtcaag
     2521 tctttcaaag ctgcagaaac ttgagcacct acaaattcta ctgctttacc agcttcgtaa
     2581 gtttcaagtg actcaccaca acagatgatt ggagtcaaac cgttacggaa gattgcatga
     2641 gcttttttat taatatcttc atcagtttcg tggaagtaat cacgacgctc tgagtgaccg
     2701 ataacaacat agttaacacc catttcagcc aatacttttg gagatgtttc accagtgaag
     2761 gcaccagcat cttcaaagta gcagttttga gcagcaactt tcaattcaga atctttagcg
     2821 aaccataaga gagcgttcaa atctacagct ggagctgcga tagctgcttc aattttgtcg
     2881 cctgcaggca attttcctgc aattgcctca acgaaagctt gtgcttcttg aggatttttg
     2941 ttcattttcc agttaccggc aatgattggt ttacgtgaca tttcacttac ctcttctata
     3001 tttattttac aaggtacatt atagcataaa cttagctaag attaaagaat ttctgtgcat
     3061 tttatgaata cttacagaaa aactctctac ccgaaagtag agagttacaa ttaagttgtc
     3121 tgactataat tgttatttct gggaactcaa tcgaattaag cttcgatttc tgtaaccata
     3181 cctgaaccaa cagtacgtcc accttcacgg atagagaaag tagtaccttg ttcaacggcg
     3241 attgggtgga tcaattcaac gtcgatagta acgttatcac caggcattac catttcagta
     3301 ccttctggca atttgattga accagttacg tcagttgtac ggaagtagaa ctgtggacgg
     3361 tagttgtcga agaatggagt gtgacgtcca ccttcttctt tagtaaggat gtaaacttca
     3421 cctttgaatt tagtgtgtgg gttgatagaa cctggtttag agataacttg accacgttcg
     3481 atttcatcac gttgtacacc acgaagaagc acaccaacgt tatcgccggc aagaccttcg
     3541 tcaagttgtt tacggaacat ttcaacacca gtaacaactg ctttagattt ttcttcttga
     3601 agaccaacga tttcgatttc gtcgttgaca cgaacagtac cacggtcgat acgtcctgaa
     3661 gctacagtac cacgaccagt gattgagaat acgtcctcga ctggaagcaa caatggtttg
     3721 tcagtgtcgc gttctggttc tggaatgtac tcatcaacag tgttcatcaa ttccataacg
     3781 atgtcttcgt acttagagtc accttcaaga gctttaagag ctgaaccttg gataactgga
     3841 agatcatcac ctgggaaatc gtattctgaa agaaggtcac ggatttccat ttcaaccaac
     3901 tcaagcaatt cttcatcgtc aaccaagtca actttgttca tgaagacgat aaggtgttta
     3961 acaccaacct gacgtgaaag aaggatgtgc tcacgagttt gtggcattgg accgtcagtt
     4021 gaagctacta caaggatcgc accgtccatc tgagcagcac cagtgatcat gtttttaacg
     4081 tagtccgcgt gtcctggagc gtcgatgtga gcatagtgac gtttttcagt ttcgtactca
     4141 acgtgtgcag tgttgatagt gataccgcgc tcgcgctctt ctggagcagc atcgatagac
     4201 gcatagtctt taggttggtt aactgatgaa ggcaagcgac gtgccaatac agttgtgata
     4261 gctgctgtca aagtagtttt accgtggtca acgtgtccaa ttgtaccaat gttaacgtgt
     4321 ggtttactac gatcgtattt ttcttttgcc attttggtaa aagcctccaa taaaatatat
     4381 tttatagata gacagtaggc aatacagtct aactttacct tactattcta tcaaattgta
     4441 gcagaattgc aagcattttc tatcgttttt tattttatcc attttctgca agggctatta
     4501 gcttcgctta taactttttt tatcatccca ttttaaccta ttactcaact aaaaacacct
     4561 catttccgta agaatattga tgttaccata gctacaaggt gccacaaata cgagaccact
     4621 gcgacagacc tttctccatt cagttatcct acgactccct gacaagcctc ttgctatagg
     4681 tgttgaaaat atattgacta ttttcactta atgatgcagt tgataataag gctgaatagg
     4741 aatggacgcg ccaatactag aagaataaat ccagtccttg ccttccaact gggcaaagtc
     4801 ttctgctttt cctgttagga taatgcctgc aaattctagc ttatcaatct cttcttgttt
     4861 agtaaagtct gtatcgccaa atttgtcaac aaaggcttgc aagtcatcca caggtatgtc
     4921 cagatactgc gctcgtttct gatctcccga ttctaagaac tctttcataa agtccatacc
     4981 tgtcatatag tctccaggca tatccgaaaa gactagttga atatcctctg gatctaaccc
     5041 caaaagctgc tctggacgat agtaatgggt attctgcttc ccttctgtcc ctatccaata
     5101 ccaattttgc ttgagattgc tgggaatcat ttgcttgatt tctgccaaac tatacttctt
     5161 atcaaaggaa atagccactt ctactagctg cccactcatg tctttaagat aatgcaattc
     5221 attggcaatg tcttgaacac tccaaacgga cttttttcta ttgtaaaaaa caggtatctt
     5281 ggtattggtt gtgaaatcat atagatattt gaaggtattc ccgggtatat gctgagcaaa
     5341 atcatagtga cgatcaaaca ggtcatagta aatttcgtgc tgaccaaaag ccataggaac
     5401 accagctaga tccttatata aattgcagta aagtttccca gaaacataac tcgtgctttg
     5461 gattttaata ccgtcatgtt ttatatttgg ataagagatt tccattaaaa taatttgttt
     5521 ccaattctca ctaccttctc gtgcaccgac aattattttt acagtttgaa tgccaatcaa
     5581 caataagata aagccgatga acgatgatag tccaactgtc ttccataaat ttttccgctt
     5641 actctttttt gtcacctctt caaaagtctt catagtcata tcctctctct tctagtattt
     5701 tccttaactg cttgcgtcct cggtgaaggc tgattttcac ccaactttga ccagcaccta
     5761 aaatatgcgc aatctctttg atactcatat cttggaagta gtataagtca atcacttgct
     5821 ggtatttttc tggcaggtct gcaacagctt gatagagttc ctcataatct tcttggtcat
     5881 agaccaaaaa attttcaggt gtaaaaaact ccttttgtaa aatttcatgg tagcgcttgt
     5941 ctcgcctgta tttgtcaatg taagcccgaa ctgcagaacg gtaaagccaa gctcttagtt
     6001 tttcgaaagg aaggacaagc tctgcttcta aaattttcac tagtatatcc tgtgcgatgt
     6061 cctgactatc tgcatagttg gcgccagatt tttgtagata gtaggaaatc tcctcagcaa
     6121 taccaatgac ttctttttca tattgatcaa gtttgatact catctcctcc tttcactaat
     6181 agaacgaata agggatgatt tggttacact ttcttatgat tattatatat ttttatcagc
     6241 aaaaaaaaca acctccgaag aagttgtttt atgcctatta acctgagttg cgcaaacctg
     6301 tcgccacacc attgatggta atatgaatga gattctcttg ttctttgctc aattctccac
     6361 gacgaagacg atgaattaat tcaatctgaa tatagttgag cacattaaaa taaggcatgc
     6421 gataatcaag actagctttt agaaacggta gttctgctaa aagctcatca ttttgctcga
     6481 ttgacaaaat aatttctttg gtcaactgcc attcatctaa aatgacatgg aagatatttc
     6541 gtacctcttc gctttcacac attttagcat attcaaatgc gatattcata ttggatttag
     6601 acaataccat atccacgttt gatagaagtg aacggaagaa tggccaagat tcatacattt
     6661 tttgtaactt agacaaatta tcagggtgct tatcaataaa gcgtttgaaa cttgaaccaa
     6721 ctccatacca ccctggcaac atgacacggt tttgtgacca agagaatacc catgggatcg
     6781 cacgcaaacc accgatttct gtgatggttt ttcgagccgc aggacgtgag ccaatgttca
     6841 gacttgatac ttcacgaatt ggactagcgg caaagaaata atcataaaaa tgtggattgt
     6901 tgaatacaag atctctataa atacgatagc tatctgatac aatttcatcc atggtttcgc
     6961 gatagccatc gatttcattt ggatcggtaa tcatttgtgt gaccatacgg tcaagcgttg
     7021 cagataccag catttctaaa ttatagtagg ctgcgtcttt gttaccgtat ttattgccta
     7081 tcacttcgcc ttgttctgtc aaacgaagcc ggtccttgat ggaaccaaat ggctgagatg
     7141 taatcgcatc ataggaagga ccaccaccac gaccaacagt acctccacga ccgtggaaga
     7201 aggtgatgtt tactccgtag tcttgaccaa tttgcgtcaa ttcattctgt gctttgtaga
     7261 gtgtccagcc ggaggatagg tagccaccat ccttgttgga atctgagtag ccaagcatga
     7321 tttcttggta gtatttattg cctgcaatcc aacgtttcgc taaatctaat tgcagatatt
     7381 gacgcatggt atcaggtgca ttatctaaat cctcgattgt ctcaaaaagt ggaacaatct
     7441 gaacacgggc cttatcgaca tctactagac caacttcttt taataggaca gctaattcca
     7501 gtaaatcaga aacactctcc gagtgtgaaa taatatgttg cttgatgatt tcctcaccta
     7561 gcttatcttt caattcacgt gcagcagcaa agattgccaa ttctttttct aattgttctg
     7621 actttggcat gtgcgttgca gaaagaatgc gtggatctgt ttccaactgt ttcaaaagaa
     7681 catggcattt agcttcctca gataaactgg agtagtcttc tacgatacga gcagaggcca
     7741 ataactccgc aacacttgct tcatgaatag aagagtcttg gcgcatatcg atactagcta
     7801 ggtagaaacc aaacgcttca accgcttcaa gcaattctgt aaaatcacct ttcaatagcg
     7861 ccatagattt attttcgacc aaagattgtt tgatggctaa taaatcagct ttaaactcgg
     7921 ctacattact atagcgaaca tctgacggct tattgtgaac taaataatct cttgtatttg
     7981 caagtttcat ctgaatatag tgaaaagcac gacgataagg ctcattttca cggtagacag
     8041 aactatcttc agataaatcc gccagcatct taaccgctgt agatacattt acaatggaag
     8101 ttgagagcga aaaattccga tagagtttat agagttgctc atcgtaataa ttcataatga
     8161 cttcacactg agtcaaggct gacagcttca aggtctctgc agtaacaaat ggatttccat
     8221 cacggtctcc cccaatccac atacccattg taattggacg aggattattt aagacgattc
     8281 ctttctcagc ggccaaacgt ttgtattccc gctgcaactt agtgacagcc ttaataaagg
     8341 aagaattgta atactccata acgtttgtga tttcgttggt taccttcaac ttcttctcac
     8401 gaatcatatc cgtctgcatg ataatttcaa tgtaacaacg gagctcatct tcccatttgt
     8461 ttttattgag caagcccaat tttacatcgc gataacgacg aagcaactca tgaatatgct
     8521 tggttaaatc aagcatagac tggcgttgaa cctgagtagg atgggctgtc aagactggaa
     8581 caacattcaa cttttcaagg atttcagctg cattttcttg tttggaaacc atatcaattg
     8641 ttgttgaaat cttaccaaga taatcttgat caatattgtt ttgatggttg atttcatagg
     8701 ccaagtcaac atcttctgat atattaatca aaagtggcaa aacagcaaag tatcgagaaa
     8761 taaccgctag ttcatcatta ttcaactgac tgataatagc aatcaatgcc tgataatcat
     8821 cttgacgaga caattgagat agttctacaa ttttagcaaa agtctctgca ggcatcattt
     8881 gggctgctac ttcttccaaa atagatgtta gaatcgttac ttcttcacgg attgcttcct
     8941 tgttgttata gttttctaat ttttgaatag ccatctccta cctctttcct atctctaaat
     9001 tagcgtacgc tctatttctt cttcaattaa tcgacgtttt tcactagcat caatattcaa
     9061 aacaaaccct atcccgatgg aaataatcaa gagactcgat ccaccctgtg acaagaaggg
     9121 gaaagttacc cccgttgcag gaataattcc tgaaattcct ccgatattga caaatgtttg
     9181 aatcagaagc atacctgcca ttccaagtgc catcatcgca ttaaatggac tacgagcacg
     9241 gacgcctaca ataataattc gaataatcaa aaagaacaat aaggctagaa ttaaactagc
     9301 tccaacaaaa ccgagttctt caatgacaat cgaaaacgca aagtcggtgt gggcctctgg
     9361 tagatacccc tttttctcaa tcgaatttcc caaaccacgt ccaagccatc caccgttact
     9421 catcgcatag taagaatgag tcaattgaag acctgagttg gtggcattac caaatggatc
     9481 aaaataggca gcaaaacgac gagcaatata accaaagagc ggaatttttt ccaccgtttc
     9541 tacacccaaa atacgaatcg tccctagaat aactgttgag gcggccacaa ttcctaaaac
     9601 cgctgttgaa aaccagcgat aggcaatccc actcacacta atcatcacga ccataatcag
     9661 cacaataatc gtcgcatttc ccaagtccgg cagtgtcgca actagaccta ggaggagcag
     9721 actgaccacc cgccaatctg taaactcttt cggaatccaa cttcctttaa tcaacgatac
     9781 atagtcataa gtccgaatat ccgcctgttg ttttgcaaac tcatgtgcca gataccaaat
     9841 gataattaat ttcaaatatt ctgccggctg caaggttcct gcgattggca gaggaatcca
     9901 tccatgagcc ccgttaatcg gaggaaacaa ccgagaaata gctaacagaa atacttctac
     9961 caaaataacc gaatagataa cggctttctt gcgtaaaaaa cttaatttca tacgataaat
    10021 cgtatagatt gctaccaagc tgacaatcca gaatcctgcc tggttaatca ctgttctaaa
    10081 tggatttcca ccattaataa tttgcgtagc actggttgtc gaatagacca tgaccaaccc
    10141 aatcaccgat aagatgagat agggaatcag tatggaataa ttcaacaact ggcgtttgtc
    10201 aatcttcatt gcttatccaa ccttcttcga gtatagctct tattatagca tactttgtat
    10261 aaaaatactt atcactttct aagttgctct gcaaacattc ggaactgact tttgtcgaca
    10321 agaaaaaggc cgaagccttt catatctatt caaatgtttc aagataaaat tcaatttcat
    10381 ccccattcgc aactgtaagg tctttcgccc ctttatcagc catttggcca ttaactttat
    10441 acatccagta tgtattggta ctcgtatctt ggctaacacc atcaatttct gttatcaaac
    10501 cattgtcttc tacaatgtca tgatgttctt taagcacatc catgacacta tcaccttctt
    10561 ccgcagtgac ttcctgacga ttctctttgt ccttggttgt aatctgcaaa gtgacttcga
    10621 tagccgagtt gttctgtttc tcggtagtgt ttgtacaagc agccaagaag agcgagaaga
    10681 caaccatcaa cataatacta atttttttca tttataaaat ctcctaaaaa tataatcaat
    10741 aattggataa aacaaaactg ttgaagtagc gtgtaaacta tcaaagacta aaccattaag
    10801 ccaaaagacg ataggagaag tcccaaactg tgtagcagat aaaaaactta tcgttagccc
    10861 atacaaaaag acaagaatcc ccgccagaag actctgtagc cacaagggaa tgttctcttt
    10921 tttgataaaa ggaccgattg ccaaccacca tataaattgg ataaaagcaa aactaagaac
    10981 ttgccacaaa acaactgtgc caaatccaaa aagcaagcca ctccctacca tcactagtat
    11041 catcaatatc cagctctcca gcaaactgaa ataggacaga acaactagct gactaacacg
    11101 tcttactttt tcacttttgt catgcatacc aatttagcct accctattcc ctttattatg
    11161 tcaagttcag ataatctttt actatttctt gaatcgtatt tttttgattt tttcatgatt
    11221 ttacggtaga attgaggaga tgagaaagaa actaaatccg atgattgttg ttggtttttt
    11281 cctatccttc tttgctctta ttttcatcac tggggtgact ggaaatactg taaataagac
    11341 ggaggtagct aacgaacccg cagtcacaca atcaaacacc agtacaagtt ctaaaacaac
    11401 aaattctagt tcgagttatt acattgcaaa tgcgctcgaa atgaaaccta tcatcgacgt
    11461 ttctgcttgg cagagaccgt ctgaaattga ctacgatacc cttagcaaaa acatttctgg
    11521 cgccatcgtt cgtattcaaa gtggatcaca cactaaaaac gaaaatactg cgactgataa
    11581 aaatgggtta gataaatcct ttgataccca tattaaagaa tttcaagcaa gaaatatacc
    11641 tgttgctgtc tatgcctatg tcacgggaaa tagtattgag aacatgcgag aggaagctcg
    11701 aagcttttat aaggccgcaa ataagtacaa gccaaccttc tattggttag acgtcgaaga
    11761 atataccatg gatgatatga atgctggagt ggaggctttt cgaaaagagt tagaaactct
    11821 cggcgctaaa aacattggaa tttacgtcgg aacctatttc atggaagacc acagcatcaa
    11881 tgtggataaa tttgacgcta tttggattcc tacatacggt gatgattctg gctactacaa
    11941 tgccgctcca aatactgact tgaactacga tcttcaccaa tatacctcac gtggttatgt
    12001 aaacggcttt gagcaccacc tagacctcaa cattattact accctgaagg atcctaatga
    12061 agtttatcag aaactattta caacacccga ataaaatgat acaatttcac taattacata
    12121 tacacaaagg agtgcttcgg ctgagatcgc aactgcgaaa tcctcgaacc tgatctagtt
    12181 aaaactagcg taggaattgt gtgctttata gaatgatact atactctgct ataaacactc
    12241 ctttgttgac catctacaaa ggagtctttt tatgcccaca tcaaaattac ccatcctagc
    12301 tgaagttgct atcttttctg ccattgccct cgtctttgac aaaatcccgc tctttacaat
    12361 gccccaaggt ggatctgtgt ctttagtcat gcttccaatc ttactgcttg ctctgcgcca
    12421 cggactaggg gtaggagttc ttactggtgg tattgtagga accattcaac ttttctacgg
    12481 tggctacttc ctaaacgttt ttcaagtttt tctcgactac gcattttctt acgccggaat
    12541 tggtttagca ggtttagtgg cacccaccct atccaaacaa aacaatctaa aaaatgctac
    12601 tctcatcatt actctggcta gttttctagg aggaagcatc cgattgttag ccaccttctt
    12661 atcaggaatt attttctacg cagactacgc tccagatggt atgcctgtct ggttctattc
    12721 ctttacctac aatatcagct atattcttcc ttccacagca attgcctcta ttctgctcat
    12781 tttactctat agagcaagac caggttttta caatctttaa ccaaaactgc ctgctggatt
    12841 ttatctaaca ggcagtcttt ttaattattt ttacttgctt tatgctataa taggaacgtt
    12901 ataatacatt ttggaggaaa ttatggcttc ttggatttca agaattatta aaggtatgat
    12961 tatcgcactc ggctttatct tacctggtgt ctctgggggg gtactcgctg ctatcctcgg
    13021 aatctatgag cgactaattg gatttttagc aaatattcga aaagatttca aggaaaactt
    13081 tctttatttt gttcctgtag gaattggtgg aattttggga atcgcacttt tctctttccc
    13141 tgtagaattt ttattgcaac atttccaagt cccagttttg tgggcctttg cgggagcaat
    13201 cgtgggaacg attcctagcc tacttaaaga atcaactcaa aagagcaaac gagatagcat
    13261 tgatttggct tggttgattg ggacattcat tatttctggc ctcctacttt acaatttgag
    13321 cgatcttgtc gggacacttc cagctacttt tgctagtttt gtattggcag gtgccttgat
    13381 tgctttaggg gtacttgttc ctggactcag cccttcaaac ttactattga ttttgggtat
    13441 ctacacgcca atgctaaatg gtttcaaatc acttgatcta ttcggtacct tcctaccaat
    13501 cgcaatcggt ggcgttcttg caatggttgc attttcaaaa gcaatggacc acgctctgaa
    13561 agtataccat tcacgtgttt atcactttat tattggtatc gtttcatctt ctactctcct
    13621 aattttgatt ccacaagcaa gtaataaaga gtctatttcc tatgctggcg ctaactttgt
    13681 tacatggatt gctgctattg tcctatttat actaggagca tggttagggc tttggatgag
    13741 taaacttgag gaaaaatata aataatggca aaaaaggtta aaataaagaa aaccttggtg
    13801 gaccaaatat tggacaaggc taagatcaat catgatagcc ttgttttaaa tgccttcgaa
    13861 ggtcaacttc caccaggaat tgaagaacac gaaatttaca aaacactggc attaactgga
    13921 gataaaactg gaccaattat cggcattatc ccaatcacag aacacctgtc tgagaaaaaa
    13981 ttagccaaga tctctggcaa taaaaaagtg agtatgattc cgcaaaagga cttggaaaag
    14041 acaactggct acatccatgg tgccaacaat cccgtaggca ttcgtcagaa gcataatttc
    14101 ccaatctaca ttgatcaatc tgcccttgaa ctcggtcact tgattgtctc tgctggagaa
    14161 attggtcggt cgattcgaat tgacagtcag gtattagctg attttgtaaa agctgacttt
    14221 gccgatttga tcgaggggag ggactagact tgaaaatcta ctttgtccgt cacggtaaga
    14281 ccgaatggaa tttggaggga cgctttcaag gctattctgg agattcagct ctcttgcctg
    14341 aatcttatca ggatttagaa aaactaggca aataccttgc tgaaatcccc tttgatgcta
    14401 tttattctag tgatttgcag cgcgcacata gcactgccgt agaaattgcc aaagccaatc
    14461 accattgcca aaccgttcta accacgcaac aattacgtga atggaatttt ggaacgctgg
    14521 aaggaagcaa gatggcaatt tttcgtgcca tctatcccaa acaagcctgg gcactcaaac
    14581 ataatctagc actttttaac aatgacttat ttgaagctga gagcgttcgc caggtaactc
    14641 aacgaatggt ggactttgtc caatcgctga aagggcaaga catggaaaca gtcctcatcg
    14701 tcagccatgg tgcttttcta accgcttcta tccatcgcct acttggtttt ccaccagccc
    14761 aactgcgtca ccgaggtggt ttggataatg catcggttac tattttagaa acaagcgatt
    14821 tcgaaaactt tacagaatta gcttggaatg ataccagcta taaagcaaaa cagtagaatt
    14881 ttctgctgtt ttttcctatc ctattagcaa tttatactga ttttttctct cttctatgat
    14941 aaaatagaaa taacagactt tggaggaata aaatttttat gtcaactgaa cattttgaag
    15001 aattaaatga ccaacagatt gtccgtcgtg agaaaatgac ggctcttgct gaacaaggca
    15061 ttgacccatt tggaaaacgt tttgagcgtt ctgccaactc agctgaattg aaggcccagt
    15121 acgaagacaa atcaaaagaa gacttggaag aattagggca aacagcaatc attgcaggtc
    15181 gtattatgac caagcgtggt aagggtaagg caggttttgc tcacattcaa gaccgcgaag
    15241 gccagattca gatctacgtt cgtaaggatg atgttagcga agaaaactat gaaatcttca
    15301 aaaaagcaga cctaggtgac tttatcggtg tcgagggaga tgtcttcaag acaaacgttg
    15361 gtgagttgtc tatccatgct cgcaagttga ctcacttgtc taaagcgctc cgcccattgc
    15421 cagaaaaatt ccacggtttg acagacatcg agacccgcta ccgcaaacgt tatttggact
    15481 tgattactaa ccgcgagagc tttgatcgct ttgtgacccg ctcaaaaatt atctcagaaa
    15541 tccgccgtta ccttgacgga cttggtttct tggaagtgga aacaccagtt cttcacaacg
    15601 aagctggtgg tgctgcagct cgtccattca tcacccacca caatgcccaa aacattgaca
    15661 tggtccttcg tatcgcgaca gaactccacc tcaaacgcct gattgtcggc ggtatggaac
    15721 gcgtttatga aatcggccgc atcttccgta acgaaggtat ggatgcgacc cacaaccctg
    15781 agttcacttc cattgaggtt taccaagctt atgcagacta ccaagacatc atggacttga
    15841 ccgaaggtat tatccagcac actgccaagg ctgttgtcgg tgatggtccg gtgacttatc
    15901 agggcactga aattgctatc cacgagccat tcaagcgtat ccacatggtt gacgctatta
    15961 aggaacaaac tggcgtggac ttctggcaag agatgagctt cgaggaagca aaagcccttg
    16021 cagctgagca caaggtccct gtggaaaaac accatacaga agttggtcaa atcatcaaca
    16081 gcttctttga agaatacgtt gaagcaactc ttatccaacc aacctttgtc tatggtcacc
    16141 cagtagccgt atctccactg gctaagaaaa acgatgaaga cccacgcttc acagatcgct
    16201 tcgagctctt catcatgacc aaggaatacg gaaatgcctt tacagaattg aacgacccaa
    16261 tcgaccaatt ggaacgcttt gaagcccaag ccaaggctaa agaactcggt gacgacgaag
    16321 cgacaggcgt tgactacgac tacatcgaag ccctcgaata cggtatgcca ccaacaggtg
    16381 gacttggaat cggtatcgac cgcctctgca tgctcttgac agatacaact actattcggg
    16441 atgtgttgct attccctact atgaagtaag caagagtagc ttgctaggtt tgttttcact
    16501 aggatgttca aatcgctctc cagtttcttg gcgattggct gaaaaggtct accagacctt
    16561 ctcactcccg acgatgaaat aaaaattaca aactgtttaa caccgttacg taaaacactc
    16621 ttggaaattc caagagtgtt tttgcattca aaaatcccaa ccacatggtc aggtttttca
    16681 ggagattatg aaaaatattt aggattggga gtgggaaaga actcaaataa tcatagagag
    16741 ttcgtcaaca agttcatatt tccagttgtt gagctgaaac agtctatccc cagactgttt
    16801 cactcccacc cccgcacagc tccaaaggtt tggggaacct ttggaggtcg gagatagagc
    16861 gaacagagtt cgctacagaa cagtcagaat agtgactgtt ggaggtcgga aataaagcga
    16921 acaccgttcg cttcagccgt agaggaagat gtggagtgta aaacacgaac aaatcttcca
    16981 atcaaccact gcgctgagat gttgactcca ctttagagaa gtgaggctat tataacgtcc
    17041 catccttttt atctcatcag tcctgggact gattctcttc tttttttgca aaataatctc
    17101 ggacaattgg cacaacttct tttccgtaga gttcgatagc acggaggaca tcttcatgtg
    17161 gcattgagcc gattgggaga tggaggaaga aacggtccaa gtcaagtgtt tcaatagtct
    17221 tgataatttt ttcagcaact cgttttggat ctccgacgat ggttgctcct tcgtctgtca
    17281 gagagtagag atattgcgcc ttggtcatct cgctccaatg tgggcggtct ttggcaatat
    17341 tgtccgtaat tactttagtt ggatgatagt attcttccac agccttgtca tggtcgtctg
    17401 caatccaacc ccaagaatgg gctgcaactt tcgttttctt tggatcatga cctgagttgc
    17461 gagcaacctt gcgataagca tcgactaaag gtttaaagcg atgggcacct gcaccaatga
    17521 ctgcatacac gattggcagg cctttcttag caatcttgat ggtggactct acattgcccc
    17581 cagttgcaac ccagatcgga aaatcttcct gaacaggacg tggatagact ggcttattat
    17641 cgactgactg ggtaaaatgc ccatcccact tgagattggt ttccttgccg atttccagaa
    17701 gcatatctag cttttcatcg aagagttctt catagtcgtg gagatcatag ccaaacaagg
    17761 ggaatgactc cgtaaaggaa ccacgacccg ccatgatttc cgctctgcca tttgacaaag
    17821 catcaatggt ggtgtactgc tgatagagcc ttacaggatc catggaggaa aggattgaaa
    17881 ctgctgatgt tagcttgata tgcttggtat tgacggcacc agctgccagc acaatctcag
    17941 gtgcagaaac cgcaaaatct tctcggtgat gctccccaat cccataaaca tcaagtccca
    18001 ccgcatcagc tagttcgatt tctttgacta gctggcgaat ccgctcgtcg tggctgtaag
    18061 tttcccctgt tttttctagt ggagttgttt cgccaaatgt tgaaatacct aattctacca
    18121 tgatgtgttc tcctttgaaa tcactatttc ttaattgatg cgtttatttt atcgctaatt
    18181 agtgataaat tcaattaatt tgctcaaaaa ataaaacgac taggaaaacc taatcgtttc
    18241 aatagattat tcatagcgca aagcttcaat tggatcaagc ttagatgctt tgttggcagg
    18301 tagtacgcca aatacaatac caactactgc tgagaaagcc agactaccta acacaacagg
    18361 aattgaaatt tcagcagcta ttctttcgcc caacgccttg ctcacattga gcaagaaaac
    18421 tattgtctga gcgattgcga gaccaattgc tccaccaaga gtggtcaaga ccatggcttc
    18481 tatcaagaat tgaatcaaga tatttcctct agttgctcca agcgcttttc gaagaccgat
    18541 ttcacgagtc cgctcagtta ctgataccag catgatattc atgacaccga taccaccaac
    18601 tagcaaggag atacctgcaa cagcaccgat aaacaatgta acgccagaca tttgagattt
    18661 aaactcctcc aacatagctg acatatcata aatctcgtat ctcgcttctt tcaatccagt
    18721 cgctttagtc aggtaatctg cagcagcctt cccaacttct gttgcccgag taacatcttc
    18781 aacatggaca aagatttgag aattttcttt catgcctgtt tcagcagcta gctgcgtatt
    18841 agtcatgacg gcatttccac ctgaattcat tccataaata gctgtgcctg ctttcggatc
    18901 tttgtagacc cctattacta gataggcatt gctattaaca ctaatcgtct gattgagaga
    18961 gttctctata gaaccaaata acttctctgc taatgccgta tctaacataa tcatacgtga
    19021 aaaacgacta tagtcgttag ctgtaaactg acgaccagct aaaatctcat actctttcac
    19081 agcaaagaaa ttctggctaa caccagtaat agtgacattc tctgcttttt tattgcccga
    19141 tctgacttca gctatgttgc tgatgctgga gtaatagttt gatacgccat caatctctag
    19201 cagtcctttt aaagtagaat ccgtcaaatc cggctcttcc tctaaaattg gttcttctgt
    19261 cgacgctgtg ccaaacggac cgaaagctgt acctgaactg gagaaagcag taaagtaaac
    19321 ctgaacatta ttttgatcac cagctagaga cttttctatg cttttggcca cccctgttcc
    19381 catggcgaca ataatgacaa ctgcagccac accaatgatg atacctaaca tagtaaggag
    19441 cgatctcatt ttatgagcca taatagattt gagggcgaat ttccaatttt ccatctaaac
    19501 tccctccctt cgactatctt cagtgatcac tccatcacgt agaacgattt ttcgttttgc
    19561 gtaggcagca atctcaggct catgcgtaac catgataatg gtctttcctt cattattcaa
    19621 ctccgtcaga agttgcataa tctgctcacc tgtcttggta tctagagccc ctgtaggttc
    19681 atccgctaaa ataatagctg gggtattgac tagtgcacga gcaatagcta cccgttgttt
    19741 ttgaccacca gacaattctg atggtaaatg cgtcatccgc tctgccaact caactttttc
    19801 aagatactgt tttgctagtt ccttacgttt gcttgatgga actccagcat aaatcaaggg
    19861 taattctaca ttttgaaggg cgttaagttt cgataagagg aaaaattgtt gaaagacgaa
    19921 accgatttgg tcattccgaa cctgagctaa ttttttctca gaaagttgac tgacttcttc
    19981 accttccagc caatagtttc ctgaactgaa acgatccaaa agcccaataa tgttcatcaa
    20041 ggttgatttc cctgaaccag agggccccat aatggcaaga aattctccct cttccacctc
    20101 taaatcaata ttctttaaaa ctcggagttc ttgatcacca tttttgtagg atttattgat
    20161 acttgttagt ctaataagct ggttcttcat tggcttcaac ctctttgcca tcttcgagat
    20221 tcggagttgg aatggtaata accttatctc ctgctgctat accagcggtc acttcctggt
    20281 tcaaagcatc tgcgttcccc agagtaactt ctactttctt agctttccca tcaaccaagg
    20341 tccacacata gtttttatct tcttctggca caactgcgga caccggaatt aaaatgtttt
    20401 tacttgtatt gacaacctca atatttacag aaaatccttg tttcaactca cctaattcac
    20461 tagttaaagc tgccttaaat ggatatttgg cacctgaacc actggttcct gttgtagcag
    20521 caccagcttg attactagat ggatagtttg aaatataggt aattttcccc gtccaagtct
    20581 tatcgggata taccttgcta gtcagcttga cctcttgatc cactgcaatg ttagccaaat
    20641 cgtattctgt caaactacct gttacttgca aactaccttg atttacaatg tgtacaactg
    20701 tttggctcga tgtagaattc ttagaaacag ccttattcac ctcaacaact gtaccagcaa
    20761 cggtactagt tactgtcgct tctgttaagg ctgtatatgc tttatcaacc gcagcttgag
    20821 catctgcgta tgaatcattc aagtctgcca actgcatatc tactgaacgt tgtgcagtag
    20881 cagttgctgc agcatctgtt tctgcatcac cagttgtttg aacagtctga ccgttggttc
    20941 ttaagtctgc aatttggcga ccaatcttat cacgaccacg aacagcagta tccaaagcag
    21001 actgtaattc tgtagcatca tagcgaatca aaggggtacc tacttcgacc ttagcacctg
    21061 gagcaaccaa aacctcactc aaatctccct tagaaggatc atagtaaaca tactgctcgt
    21121 ctgctgctgt aatcgttcct gtaagtagcg ttgaggaagc aattgagcct tctttagcaa
    21181 cagtataggt catactcatg gctacatctt ctgaagatga tggtgatgtt agcatattgc
    21241 taaaaatcaa agccccacct actaaggcaa ctgcgaccac tccaagacca ccaaacaaag
    21301 caatttttgc tttctttgtt tttaacataa caaactcctt ttctttacaa aacataccta
    21361 tttgtgttgt ctatataata cacaaataca agaacaaatg caagtttttt cctaactatt
    21421 ttataaaaag ttcggcaatt ttcccactta catttttgta aggtgacgta gcagattttt
    21481 gattgacata caaaaaagcg ttacaatgaa tataattatt gacctttatt ggaggaaatt
    21541 tatgaaagtg tttgatatta tcgctatcgg tggtgggagc ggaggcatcg ctaccatgaa
    21601 ccgagctgct atgtacggtg ccaaggcagc agttattgag ggaagctatc taggagggac
    21661 ttgtgttaac cttggctgtg tacccaaaaa aattatgtgg tacggctctc aggtagcaga
    21721 agccattcag cattatggac ctgaatatgg atttactagc caagaagtga cctttgattt
    21781 tgccaccttg cgtaaaaatc gcgaagccta tattgagcgt tcacgcgcat cctacggtaa
    21841 tacttttaac aataacgaag tagaagttat ccagggtttc gcacgttttg tggataatca
    21901 aacggttgaa gtcaatggtg aactgattcg tgcaaaacac attgtcatca caactggcgc
    21961 caaaccggta gtccctgcta ttcctggtag tgagctaggg attgtatctg acgatgtctt
    22021 tgcatgggaa gaattaccct cttctgtggc tgtgattggt gctggttata tcgctgtgga
    22081 aatggccggt ctcctacatg gactcggtgt ccaaactgac ctatttgttc gtggtaatcg
    22141 ccctcttcgt agctttgata cctacattgt tgatgccttg atggaagaaa tgcaacgtac
    22201 caaccttcca ctccataccg ggaaaactcc tgtcagtctt gaaaaaactg atgatggaca
    22261 aatccagatt aactttgctg acggtagtaa ccatattgct cagaaggtcc tttgggcaat
    22321 tggacgtaaa ccaaatattg acaaactcaa cctgaaagca actagtgttc aacttacttc
    22381 ttcaggacat atttctgtca atgaatacca agaaacagct gtaccaggca tctatgccct
    22441 cggtgatgta actggtgaaa aagaattaac acccgttgca atcaaggcag gtcgcctttt
    22501 ggcagaacgt ttatttaaca ataaaccaac cgcaaaaatg gattacacaa ccatcccaac
    22561 tgtcgtcttt tctcaccccg ctatcggaac cgttggtcta actgaggacg aagcgattac
    22621 ccaatacggt caggacaatg taaaagtcta tacttcagcc tttacttcta tgtacactgc
    22681 cctagaaaac aatcgccaaa tggctaagtt taaattagta actgtgggag aaaatgaaaa
    22741 agtcgttgga ctgcacggca ttggctatgg tgtcgatgaa atgattcaag gattctcagt
    22801 tgctatcaaa atgggagcta caaaagaaga atttgatgcc gttgtcgcca tccatccgac
    22861 aggttctgaa gaatttgtta ctatgcgtta actcaattga aaaagtccag acatgaaggt
    22921 cagcactgtc tggacttttt ttatggtaat atggcaattg ctcgcactgg tgaacccgaa
    22981 gccttctccc aatgaggaaa agcgattgaa atcaatgcac ctgttggcgg tacctgatcc
    23041 aagtttgcca atacttccaa ctgataaata tcttttgata ggagatagta ttcttctgat
    23101 aaacttcctc cgtgtttggc tgcagatact cctgaatcgg tatccaatgt ttcatgacca
    23161 actgctttca cctgacgttc ttcaatcaaa tattctagag cttcatgact ccatcctggt
    23221 gtacgttgaa ctccatcttc gtctagatta cgaatggcat cttgactagg ccagcgcttt
    23281 gacaaatccg aacgaaaggc aacaaaagat tttggcgcta tcggaccata ttcagcctca
    23341 aagtctaaaa tatcctgctt agttatctca tagtcattat tttctataac agcagctgat
    23401 ttatcaataa cgtagagtgg caggagtaaa tctttcaaat caatctcatc aagataacga
    23461 gccccttcta caaaatgaat cggaggatca atatgggttc cgtactgccc cacaaccgta
    23521 aattgttcga caaagaagcc atccttatga gtaaataggg ctttcttttc gagagctggt
    23581 agcgcaggga aatgcgggct cttttcattg atttggtgag tcaagtctac ccaagtttta
    23641 gatttaagag tacggtaaat atcaagtaat tctgtcatag aaattctcac tttctttttt
    23701 tattattcta gcataggtat aggttgtttt acatataatt tattgctttt aactatataa
    23761 gtttcttatt agctgttata aaaatcacct atagagttgt tacagccgat gaaagagcat
    23821 tgactttctt atccaatctc actataattt ttgttaacca taaaatttta agaggataac
    23881 aaatgaaaac aacttctatc aaatcacttg tttatatcgc tattggaaca gccttaattt
    23941 ccaccctttc acaaatcagt ctgtctatcg gacctgtacc atttacatta caaaccttag
    24001 ctatcggctt agtagcttgt ctatacaaac ccaaggaggc aattacaagt gtcagcttat
    24061 atctagttct tggcgctatc ggtttacctg tttttgctgg tttttctggt ggctttgctg
    24121 cactcacagg accgacatct ggctttctat gggggtttct actctacact atcatcactt
    24181 ctgccctcac taaatcagac tcctcaccag ttacaatttt tctagcctgc ctacttggaa
    24241 caagtatctg tttccttctc ggctgttgtg tatttaaact agtttcaggt gccagttgga
    24301 ctgacactat cggctggact gttctgcctt tcattcttcc tgatttagct aaaattacac
    24361 tagtcatgat ttgtcatcgt ttattgcaac ctgttatcaa aaaagaagcc tattttgctt
    24421 aggcttcctt catttattta ttagcaaacg cgtcgagtag aacttgaaat tcctcattac
    24481 taagagtgat ccctttcccc atcttggtat ggtcaggact ccaggtacgg atgtcatact
    24541 tggctggtgc gccattaaaa gatactcgat taagctcctt ggtccatcct ttatcatttt
    24601 cagataagac caataatttt tcttcaattt caaatgtaaa ttc
//
LOCUS       DBNFCK010000017        24445 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.017, whole genome shotgun sequence.
ACCESSION   DBNFCK010000017 DBNFCK010000000
VERSION     DBNFCK010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 24445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24445
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.017"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            268..1023
                     /gene="ldcB"
                     /locus_tag="AC1PIW_000547"
     CDS             268..1023
                     /gene="ldcB"
                     /locus_tag="AC1PIW_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase LdcB/DacB"
                     /protein_id="HIP6605511.1"
                     /translation="MIMKRTILLCLPTILLLAACHQNINQANTTKNSSTVVSTTSSQS
                     ASTTSEQSTSSSTSAETNQEVTSTETQVNFNGSYYSVQGKYGEVIIVNKKHPLAADYA
                     PGENPEALAAFQRLIADMQALGFGISNNYSGFRSYETQSSLYQSYVNQEGQAAADRYS
                     ARAGYSEHQTGLAFDLLDTSGNLLEEPNASQWLATNAHLYGFVVRYLPGKESSTGYMA
                     ESWHVRYIGQEATDIYNSGLTLEEYFGVPGGDY"
     gene            1112..1555
                     /locus_tag="AC1PIW_000548"
     CDS             1112..1555
                     /locus_tag="AC1PIW_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIP6605512.1"
                     /translation="MLKAMEVYLVTNGNGLEAIEFYKNALGATVEQVNLFKDFLPDCP
                     AELENYVMNAQFRLNGQRFMLSDNNPEMPYTVGDNITVALITDDAETAQELYSKLSVD
                     ATAINMELQAVPWSPAYGNVTDKFGISWQINAEVEGYGQEYYAEN"
     gene            complement(1583..3193)
                     /locus_tag="AC1PIW_000549"
     CDS             complement(1583..3193)
                     /locus_tag="AC1PIW_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HIP6605513.1"
                     /translation="MSKSIIWELVKINILYSNPQLLASVKKKQSKRKDASFSAYKSVL
                     LQQVFMVLSFGLLYSVFFMGVDYSQSIGFFSLQLALFTIIAIVYGFTGFFSVFYDSKD
                     TKLYLALPLKPQEVFLAKILSAQGMVLPFLMPCLSLLLITYWQIGGPLLALASIPSFL
                     ILWILVNLVNLIIMHFVGEVLTKSPRKTLISTILMTGSSLLAFAALMFLQSQQTVSLE
                     SGGFVDFPIIPIFAGFHYLVSHTISLETLLHFGLPFILMFGLITFAFKTVIPNYFNQV
                     LAIENASTKRTNNAKSAKIPSNLNQALVKHHLSTLKDSNLMVQTMIQPIIMGVALFPS
                     FSRFTENGGLSSVEWDFFGIALLAGFLLGSLFAGSTTFIGVAMSLEKENYHFIRTLPI
                     SFKKFIIQKFWVLAAVQFALPTLLYFALALGFMKVKLILAVFFTVGIIISALLTGQIY
                     YWRDQRLLMLNWQNSNQLFGRGAGQWLIAGSIIGTMFLGAIILVVSIILAGTIGAAYV
                     SSALMTLLFVLSAALQLYIYKAYWQKLK"
     gene            complement(3190..3933)
                     /locus_tag="AC1PIW_000550"
     CDS             complement(3190..3933)
                     /locus_tag="AC1PIW_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6605514.1"
                     /translation="MIQFDHITKTYGETIALDDLNLTIESGEIFGLIGHNGAGKTTTI
                     SLLTSIIEASYGTISVDGLNLEENRDEVKKRIAYVPDSPDIFLHLTAFEYWHFMGKVY
                     GVEKDTVDDRIAKLSSLFDMTARQHEPIDSFSHGMRQKTIIIGALIPNPDIWILDEPL
                     TGLDPQASFDLKQMMKEHAQSGHTVLFSTHVLSVAEQLCDRIAILRKGQLIFVGSLDE
                     LKSQYPDKDLETIYLELAGRQQVKAGEEV"
     gene            4173..5957
                     /gene="uvrC"
                     /locus_tag="AC1PIW_000551"
     CDS             4173..5957
                     /gene="uvrC"
                     /locus_tag="AC1PIW_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HIP6605515.1"
                     /translation="MNDLIKHKLELLPDSPGCYLHKNKYGKIIYVGKAKNLRNRVRSY
                     FRGAHDTKTEALVAEIADFEFIVTDSNIEALLLEINLIQENKPRYNIMLKDDKSYPFI
                     KITKERHPRLLITRQIKKDGGQYFGPYPDVGAANQTLKLLERLYPFRKCKLPENKYCL
                     YYHLGQCLAHGDNMPSLSDYDKMAKEVAQFLTGHDDKIIKEVQDKMKAAAGNLEFEKA
                     AEYRDVLQSISTLRTKQRVMAHDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERNVNL
                     FPYYNDAGEDFLTYIGQFYRDQQHLIPSEVLIPQDIDQEAVEALVDTKVIKPQRGEKK
                     QLINLATKNARVSLEQKFNLLEKNLEKTYGAVENIGQLLGIPTPVRIEAFDNSNIMGT
                     SPVSAMVVFENGVPNKKEYRKYKINTVEGPDDYASMREVLQRRYSRVLRDGLTPPDLI
                     IIDGGQGQVNVAKQVIEQELGMSIPIAGLQKNDKHQTHELLFGNPLEVVELPRNSQEF
                     FLLHRIQDEVHRFAIEFHRQVRSKNSFSSKLDGIEGLGPKRKQTLMKHFKSIGNIQSA
                     SLQEIAEAGVPYKVAEKVKESLSSKDEI"
     gene            6014..6142
                     /locus_tag="AC1PIW_000552"
     CDS             6014..6142
                     /locus_tag="AC1PIW_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HIP6605516.1"
                     /translation="MAFGEEKHKKTTFEKVTLVIVVVMVVATLAGLILPAINALMN"
     gene            6346..7659
                     /gene="obgE"
                     /locus_tag="AC1PIW_000553"
     CDS             6346..7659
                     /gene="obgE"
                     /locus_tag="AC1PIW_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HIP6605517.1"
                     /translation="MSMFLDTAKIKVKAGKGGDGMVAFRREKYVPNGGPWGGDGGHGG
                     NVVFVVDEGLRTLMDFRYNRRFKADDGEKGMTKGMHGRGAEDLIVRVPQGTTVRDADT
                     GKIITDLVENGQEFVIAHGGRGGRGNIRFATPKNPAPEISENGEPGEERNLELELKVL
                     ADVGLVGFPSVGKSTLLSVITAAKPKIGAYHFTTIVPNLGMVRTKSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVLDMSASEGRDPYEDYVAINNELETYNLRLME
                     RPQIIVANKMDMPEAEEHLEEFKKKLATNYDEFEELPQIFPISGIAHQGLENLLEATA
                     ELLEKTPEFLLYDESDFQEEEAYYGFNPDEPEFAISRADDASWILSGDKLEKLFTMTN
                     FDRDESVMKFARQLRGMGVDEALRARGAKDGDIVRIGKFEFEFVD"
     gene            8033..8197
                     /locus_tag="AC1PIW_000554"
     CDS             8033..8197
                     /locus_tag="AC1PIW_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605518.1"
                     /translation="MGDKPISFKDKYGNFVSAADVWNAEKLEELFNRLNPNRKLRLER
                     ERLAREQEKE"
     gene            8561..12331
                     /locus_tag="AC1PIW_000555"
     CDS             8561..12331
                     /locus_tag="AC1PIW_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HIP6605519.1"
                     /translation="MRRSNKKSFDWYGTKQQFSIRKYHFGAASVLLGVSLVLGAGAQV
                     VKADETVASSEPTIASSVAPASTEAVAEEAEKTNAENTSAVATTSTEVEKAKAVLEQV
                     TSESPLLAGLGQKELAKTEDATLAKAIEDAQTKLAAAKAILADSEATVEQVEAQVAAV
                     KVANEALGNELQKYTVDGLLTAALDTVAPDTTASTLKVGDGEGTLLDSTTTATPSMAE
                     PNGAAIAPHTLRTQDGIKATSEPNWYTFESYDLYSYNKNMASSTYKGAEVDAYIRYSL
                     DNDSSTTAVLAELVSRTTGDVLEKYTIEPGESVTFSHPTKVNANNSNITVTYDTSLAS
                     ANTPGALKFSANDDVYSTIIVPAYQINTTRYVTESGKVLATYGLQTIAGQVVTPSSVR
                     VFTGYDYVATTTKAVQGPYPKGTVYLAGTVQKDTVQYKVIREIVENDQAVLKFYYLDP
                     TYKGEVDWRGTDTTGFIELLTTSPTTYKVGTIYDYNINSKITAPFTIDPTKNVMVFKE
                     SEQNEQGSKYRVIAQWSGDETTKGIYGKIYIATQVWTTKLGTNEWGWFDYSDDQAGIK
                     FNNKGFWPAGVQNTLRNATPATAVETTYIYKESSKYGDVIVEYYDTDGKQIVNSVVDT
                     PKSALGTEYNTDVDRRPASLVAADGTVYFYKEVKSDSAKTTGTVVAGTTTVKYVYEKA
                     GSVNVNFVDINGKVIKAPVSDEKDAKPGYNYDTDLDQKLASITFEGKEYKLVPAGDYP
                     VGKVGKGNNLIEVGNNTAKGIDPTTGKIEAGVNKEVTYVYRAVTGSVVVNYKDTEGNV
                     IKDPETDVSDAPVGDAYTTTDKKPNEIITKDGSRYVLVPSKTDGEENGKVIEGTITVT
                     YVYQKVANWIPEIPNVPETDRPKVPYPFDPTEPDEPIDPTTPGTNGEVPNIPYVPGYT
                     PVDPKDNTPLKPIDPNDPGKGYVPPTPENPGVDTPIPYVPVKKVVTNHVDEEGNPIAP
                     QEEGTKPNKSIPGYEFTGKTVTDEDGNTTHIYKKTPEVKNGTVVVNYVTEDGTVIKEP
                     VTDTPTSPEGTPYDTTDNKPKTITFKGEEYELVRVDGTENGKVVEGETVVTYVYRKVE
                     TPAKKVVTNHVDEEGNPVAPQEEGTKPNKSIPGYEFTGKTVTDEDGNTTHIYKKTPAK
                     KVVTNHVDEEGNPIAPQEDGTTPKRQISGYEYVRTVVDEEGNTTHIYRKLSNKPTTPE
                     KETPAKPQAGKTASGKAQLPNTGEASSVAGALGTAMLVATLAFARKRRRNED"
     gene            12622..15888
                     /gene="rexB"
                     /locus_tag="AC1PIW_000556"
     CDS             12622..15888
                     /gene="rexB"
                     /locus_tag="AC1PIW_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HIP6605520.1"
                     /translation="MKLVYTDIRNPLTQYLTEQTATFAEQGKRVFYIAPNSLSFEMER
                     KVLEYLPEQATFDIIVTRFGQLARYLMIDRKEAGQPLDDVGLAMIFFRVLSQFEDGDL
                     KVYGRLQTDFGFINQLVALYKELQRANMSILDLEAMESPDKQADLVKIFLAVTDILSK
                     EGFEHQSKLAQLTGLVETGQLDEQLKNIVLVVDGFSRFSAEEEALVSALNERVSEILI
                     GVYASKKAVQATYAEGNVYQANVDFLRQLSAQFQTKATYIGQEPVLDSIGKFSKNMEA
                     YYDYSGTMIDLTPADQEKIQLWEVVNQKEEVEQVATAIRQHVHQGARYKDILLLLGDV
                     DSYKLQIGKIFDKYDIPYYFGKAEEMSHHPLVHFVESLERLRRYRFRAEDLLNLLKSG
                     LYASISQKELDLFESYILFADMKGQAAFSRAFSVNGRADYDAEVIKEKRLVYDLTVLE
                     PLRAKIMEPLNQLFKAGPQSGTALLEKFMAFLEAIDLPKNMEKMSRNLSEVEQEKEEQ
                     VWKSFTHLLENFHQIFGKEKLKMDDFLAILQAGMQASHYRTVPATVDVVNVKSYDLIE
                     PHTAKYVYAIGMGQSNFPKVAKNTSLLTEEEMEKVNLVSASSSRFDLVSRENIKKNHA
                     AMMSLLNSATEQLVISTPQIYNEGEDSLSPYIKILQKMGLKSEERGRIKTLSPQDIGH
                     YKSLLSRLIESERPSLETEEWEGQRAFWTVLVRHLKKKLESQSIEIPTITGDIASKQL
                     SDETLAALYPEDKPLNLSASSLTNFYNNQYLYFVRNVLRLREQESIHPTAFQHGLFLH
                     RIFERVVMDQSELDFDQKVDKAILRTRDEAEFAMFYNQDADARYTEEVLDKIARSSAT
                     ILRDNDLVEIDGQEKSFRQDKALVFDLQNGKSVHVNGTIDRLDTLQINQAVGVVDYKS
                     SDQSFSVGDFYNGLKPQLVTYLAALQELDETKDKPVFGAMYLHLQDPIIKLKDTKNLE
                     QLEGAANTSLVYKGLFLKEESLGLNHFYQTRNQLYTEDEFAVLLNHNQELYKQAAMDI
                     LAGRFAINPYTKDGRSVAGEQLKAITGFEADRHMGMARRLVKEAKRQDWMERMKGGQD
                     "
     gene            15888..19538
                     /gene="addA"
                     /locus_tag="AC1PIW_000557"
     CDS             15888..19538
                     /gene="addA"
                     /locus_tag="AC1PIW_000557"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HIP6605521.1"
                     /translation="MAFEQFLSAEEIKAVQLAEAHSDKQQKRTAEQIEAIYTHGQNVL
                     VSASAGSGKTFVMVQRILDKLKRGIGIDQLFISTFTVKAAGELKERIEKKLNETIAET
                     TDMELRRHLSAQLADLTKADIGTMDSFTQKLVTTYGYSLGISPQFRILQDETEKASLK
                     KEVFDQLFADYLEEDENGAFRKLVRNFSGNRKDNSGFRQVVYQVHDFSQSTSSPTKWL
                     KEQAVQADLYSQERIEQMLEQGFKEKVLDKLYQAADFFRYHVEWGRNDFGSAKYFANV
                     EEVLDLLTGLDSLDQKDLMERVERILLINNQSRGKGLTNANRPKDEHLIAFKEEYNAG
                     KSQIISELRDLGQEVYELTLLKDYQVQALPLLILLRDFVLDFSQAYLDVKIKEAAFEF
                     GDIGHFAIRILEENADIRQFFQEKYHEVMVDEYQDNNHSQERMLDLLSNGHNRFMVGD
                     IKQSIYRFRQADPMIFQEKFELYQANPQSGKLILLKENFRSQIEVLEATNAIFTRLMD
                     RQVGEIKYDDTHSLVAGSPGQKIAQPKNEMEYLIYDQQDSANSSTDAEEETPLTAGEI
                     EVVAKEIIRLHNEEGADFKDITLLVQKRTHNDLIMSIFEKHGIPIVADGGAASYLQSL
                     EVMIMLDTLRVINNPLNDYALVALLKSPMFRFDEDELTRISLQAGTGFFYQKMEIAQQ
                     ASGQHPELMSEKLKKKITDFLSILENWRAYAKLHSIYDMIWKMFNEKFYYDYVGALPN
                     GSKRQANLYALGLRANQFEKTGYKGLSRFIAMIDRALANDKDLADVQEFLPQNAVQLM
                     TIHKSKGLEFKYVFLMNIDKRFNLEDHYQSVIISRKNGLGIQYLADMKDKVNSPLPQV
                     RVLMNTLPYQNNLQELKIANLSEQMRLLYVALTRAEKKLYLVGKGNADKLAEKYDGKK
                     ENGVLAQSTRESMATFQDWILAIDEAFSGEDLHFKKVFVTDEDLTEEKIGKLTLKSKL
                     EDASLKDIRQSEDIAQALDQLSSVQELNERYKAAIELPSLRTPSQIKKLYEPILEQEG
                     MEVMEKYQPKRTFNLPDFSKKPKITGAQVGSAVHELMQRLDLSWLVTEDTVRAALEAV
                     HAEQAIKDKINVQMILDFFDTDLGREILANTDKLHREAPFASLQTDSVSQENFVLRGI
                     IDGYLLYDDHIVLFDYKTDKYDQPIQLSQRYQAQMQLYAEALKKAYKIDRVDCHLILL
                     GGERIEVVEVNI"
     gene            complement(19660..20304)
                     /gene="plsY"
                     /locus_tag="AC1PIW_000558"
     CDS             complement(19660..20304)
                     /gene="plsY"
                     /locus_tag="AC1PIW_000558"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HIP6605522.1"
                     /translation="MLVNLILLFIAYLLGSIPSGLWIGQTFFNINIREHGSGNTGTTN
                     TFRILGPKAGTIVFVIDFLKGTLAALLPLIFHAQGISPIVFGLMAVLGHTFPIFAQFK
                     GGKAVATSAGMLLGVAPAFCLYLVLIFASSLYLTSMVSFSSVLAAALAILGALLFPAI
                     GIILPSYDWLFTVIIVFLGSFVIIRHKENIQRILKKEENLVPFGLNLTKQKKRV"
     gene            20447..22390
                     /gene="parE"
                     /locus_tag="AC1PIW_000559"
     CDS             20447..22390
                     /gene="parE"
                     /locus_tag="AC1PIW_000559"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HIP6605523.1"
                     /translation="MAKKEIDINNYNDDAIQVLEGLDAVRKRPGMYIGSTDGNGLHHM
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLSVSDRGRGMPVGMHATGKPTVEVIFTVL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFEQGGKPVTTLE
                     KIGTAPKSKTGTKVTFMPDDTIFSTTDFKFNTIAERLKESAFLLKQVTMTLTDERTGE
                     QEEYHYENGVQDFVSYLNEDKETLTPVLYFEGEDAGFQVQVAMQYNDGYSDNILSFVN
                     NVRTKDGGTHETGLKLAITKAMNDYARKTNLLKEKDKNLEGSDYREGLSAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVSDKLTFFLLENGELASNLVRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVINTAKAKMADILKNEEINTMIYTIGAGVGSDFTIEDVNYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGRVYIALPPLYKMSKGKGKAEKIAYAWSDGELED
                     LRKDFGKGFILQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVSVLMGD
                     KVEPRRKWIEDNVKFTLEESTVF"
     gene            22420..23232
                     /locus_tag="AC1PIW_000560"
     CDS             22420..23232
                     /locus_tag="AC1PIW_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATPase"
                     /protein_id="HIP6605524.1"
                     /translation="MKLQIWNESYSLQWKGTYFLALSDYPNIQDWELEKIVAFLAYEK
                     LYGRETLIDCEDKVMLEQLVYLSCCSPTAFPFTPSKKIVASTYDVGGNYVYSDFLSHT
                     CTVETAQEIFMSGQLLSAVRAFNKTHQELVQDNRNAAGDPEDYFDYVMLGWSNTSSGY
                     RLAMERLLGREPTEEELGNSFIPGVSFHFKYDDIVNADGYVFDGYHPAKVRDCLSLKY
                     LFACIVPSSEKSHFETIIPLDMSSKVYYLDYGQDGMADWSQKVYQFVENIAN"
     gene            23285..23389
                     /locus_tag="AC1PIW_000561"
                     /pseudo
     CDS             23285..23389
                     /locus_tag="AC1PIW_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
     gene            23413..24019
                     /gene="fic"
                     /locus_tag="AC1PIW_000562"
                     /pseudo
     CDS             23413..24019
                     /gene="fic"
                     /locus_tag="AC1PIW_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395946.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
     gene            24052..>24445
                     /locus_tag="AC1PIW_000563"
     CDS             24052..>24445
                     /locus_tag="AC1PIW_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HIP6605525.1"
                     /translation="MPLRVKLTDRFQKRLSEWARKRKPFKNKQELEEKFGQALKIWQS
                     SKTRQHAENLLSEQGKLEHFLHSTERKLSRMPFGGDKFAAIPGLISMVRSYIRRDYSK
                     LPKATILAIIGALIYFFSPIDALPDFILG"
BASE COUNT         7502 a         4657 c         5607 g         6679 t
ORIGIN      
        1 gtcagtatct cctaaaaaac tattttgtat ataaatcact ttacttgtga atacgactat
       61 ttaacatcat atatgtccac cagtcgaaag gctggtgttt ctatttatga catgtatgtt
      121 atcaggaatg ccactatttg aaaggaaaag aatcgagaga aattcaatga atactaaaaa
      181 tatcaatagt agttaaaatc ataagccgtc attagacggg gtcttatgat tgtgctacaa
      241 tagacataat tataagagaa cggggtgatg ataatgaaac gtactattct attatgcttg
      301 ccgacaatac tgttactagc agcatgtcat caaaatataa atcaggccaa tactacaaaa
      361 aattcgtcaa cagttgtttc gactacttca agtcagtctg cttcaacaac ttcggagcag
      421 tcaacaagca gctctacatc ggcggaaacg aatcaagaag taacaagtac agagactcag
      481 gtaaatttta acggttccta ttatagtgta caaggaaaat atggcgaagt gattattgta
      541 aataaaaagc atccgcttgc agctgattat gcaccgggcg aaaatcctga agccttagca
      601 gcttttcaac gattaatagc agatatgcag gcgctaggat ttggtatctc caataactat
      661 agtggatttc gttcctatga aactcagtca agtctctacc agtcttatgt aaatcaggaa
      721 ggacaagcag cggcagatag gtattctgca agagctggat atagtgaaca tcagacagga
      781 ttagcctttg atttattgga tacaagtggt aatttattag aagagcctaa tgcttctcag
      841 tggttggcta cgaatgcaca tctatatggc tttgttgtcc gctatcttcc aggaaaagag
      901 tctagcacag gctatatggc agaatcctgg catgtgcgat atattggtca agaagcgaca
      961 gatatttaca attcaggctt gaccttggaa gaatattttg gtgtaccagg tggagactac
     1021 taattttaca tttagtcaga aataagtcga aaaatttgtc cgtataacta actcgtgata
     1081 taataaacca tcataatttt ggaggtccat catgcttaaa gcaatggaag tatacttggt
     1141 tactaatggt aatggtttgg aagccatcga gttttataaa aatgctcttg gggcaacagt
     1201 ggagcaagta aatctattta aagatttctt accagactgc cctgctgaac tagaaaatta
     1261 cgtaatgaat gcacagtttc gtttgaatgg tcagcgtttt atgttgtcag acaacaaccc
     1321 agaaatgcct tatacagtag gtgacaatat tacagttgcg cttatcacag acgatgctga
     1381 gacagcgcag gaactataca gcaagttatc cgtagatgca actgctatca atatggaact
     1441 tcaagcagtt ccttggtctc ctgcctatgg taatgtaaca gacaagtttg gtatctcttg
     1501 gcaaatcaat gctgaagtag aaggctacgg tcaggaatat tacgcagaaa actaaaatag
     1561 attggacaag gccttgtcca atctatttta gtttttgcca ataagctttg tagatataaa
     1621 gttgaagagc agcagataga acaaatagta aggtcatcaa ggctgagctt acataagctg
     1681 caccaatagt gccagctaat atgatgctga caactagaat aatggctccg agaaacattg
     1741 ttcctatgat cgatccagca atcaaccatt gacccgctcc gcgaccaaat aactggttac
     1801 tattttgcca gttgagcatg aggagtcgct ggtcacgcca gtaataaatc tgaccagtta
     1861 gcagggctga aatgatgatg ccgactgtaa agaagactgc tagaatgagt ttgactttca
     1921 tgaaacctag agccaaagca aagtaaagta gagttggtag tgcgaattgt accgcagcca
     1981 atacccagaa tttttggatg ataaattttt tgaaactaat aggaagcgtt cgaatgaaat
     2041 gataattttc cttttccaat gacatggcta ctccgatgaa ggttgtagaa ccagcaaata
     2101 gactgccaag caagaatcca gctaatagag caataccaaa gaaatcccat tcaactgaac
     2161 ttagaccacc attctccgtg aaacggctaa aacttggaaa aagtgccacc cccatgatga
     2221 taggctgtat catggtttgg accatgaggt tgctatcttt aagagtagat aggtggtgtt
     2281 tcactaaagc ctgattgaga ttgctaggaa tcttggcaga cttagcatta ttggtccgtt
     2341 ttgtcgaagc attttcaatc gccaaaactt gattaaagta gttgggaatg acagttttaa
     2401 acgcaaacgt aataagtcca aacatcaaaa tgaaaggaag tccaaaatga aggagtgttt
     2461 ctagtgatat agtatggctg accaagtaat ggaatccagc aaatataggt atgataggaa
     2521 aatcaacaaa gccaccggat tcgagtgaaa cggtctgctg ggattgcagg aacatgagcg
     2581 cagcaaaagc cagcaaggaa gaacctgtca tcaggattgt agaaatcaat gttttacgag
     2641 ggctttttgt cagaacttcc ccaacaaagt gcatgataat cagattgact aaattaacta
     2701 aaatccagag aatcaaaaag gaaggaatgg aagctaaggc gaggagcgga ccgccaattt
     2761 gccagtaagt gattagaagt aaagacagac acggcatgag gaagggtagg accatccctt
     2821 gtgcagagag gattttagcg aggaagactt cttgtggctt caggggaagt gctaaataca
     2881 attttgtatc cttactatcg tagaaaacgg agaagaaacc tgtaaaaccg tagacaatgg
     2941 caataatggt aaagagcgct agttgcagag aaaagaaacc gatagactga ctataatcta
     3001 ctcccatgaa aaagacagag tagagaagtc cgaaactcaa aaccataaat acttgttgca
     3061 agaggacact tttataggct gaaaaggagg cgtctttgcg cttactttgt ttcttcttga
     3121 cggaagctag aagttgaggg ttggaataaa gaatattaat ctttactagt tcccaaatga
     3181 tggatttact catacttcct cacctgcctt cacttgttgg cgtccagcta attccaaata
     3241 gatggtctcc aaatctttat ctggatactg tgatttgagt tcatcaagac ttcctacgaa
     3301 aatgagttgc ccttttcgga gaatagcaat gcggtcacag agttgttcag ccactgaaag
     3361 gacgtgtgta gaaaatagaa cggtgtgacc agattgagca tgttctttca tcatttgttt
     3421 taggtcgaaa gaggcctgag gatccagtcc agttaggggt tcatccaaaa tccaaatatc
     3481 agggttaggg atcaatgcac caataataat ggttttctga cgcataccat gtgaaaaact
     3541 gtcaataggc tcatgttggc gcgctgtcat atcaaataga ctagatagtt tagcgatacg
     3601 gtcatcaact gtatcctttt ctacaccata gaccttgccc ataaagtgcc agtattcaaa
     3661 tgctgttaag tgtaggaaga tgtctggcga atctggaaca taggctatcc gttttttgac
     3721 ttcatcgcgg ttctcctcta ggtttaaacc atcaacagag atagttccat aactggcttc
     3781 gattatcgaa gtcagtagac tgattgtcgt tgtttttcct gccccattgt gaccaattaa
     3841 accgaaaatc tcgccgcttt caattgtcaa atttaggtca tcaagtgcga ttgtttcgcc
     3901 gtatgttttt gtgatatggt caaattgtat catagtaacc tccttgttat tcgtgaacta
     3961 aaagtagggg cttgctaggt attccaagcc ttattccact gttcttcgaa taatatatac
     4021 aattaattac atttattata ctgctgaatt agtacaaaag caatagtttc tatcaatatt
     4081 tttgtagtat ggatgcggat atattgcaag aagtcgagtg taaaatggaa tgcaataagc
     4141 tcgattatca gaaaatttgc tataatagat ctatgaacga tttgatcaag cataaattgg
     4201 agttactacc agatagccct ggctgttacc tccataaaaa caagtacggt aagattatct
     4261 atgtgggcaa ggccaaaaac ctacgcaatc gggtgcggtc ttattttcgt ggtgcccatg
     4321 ataccaagac agaggctcta gtggctgaaa ttgccgattt tgaatttatc gtgacggatt
     4381 ccaacatcga ggccctcttg ctagaaatca atctgattca ggaaaacaag ccacgctata
     4441 acatcatgct caaggatgac aagtcttatc ccttcatcaa gattaccaag gaacgtcatc
     4501 cacggctgct cattactcgg cagattaaga aggatggtgg tcagtatttt ggtccctatc
     4561 cagatgtggg tgcggccaac cagaccctca aactattgga gcgcctctat cctttccgca
     4621 agtgtaaatt gcctgagaac aagtattgtc tttattatca tttggggcaa tgtttggctc
     4681 atggagacaa tatgcccagc ctgtctgact atgacaagat ggccaaggaa gtggcccagt
     4741 tcttgacagg tcatgatgat aagattatca aggaagtgca ggacaagatg aaggcagctg
     4801 ctggcaattt ggaatttgaa aaggctgcgg aataccggga tgtcctccaa tccatttcaa
     4861 ccctgcggac caagcaacgt gtgatggctc atgacttgca gaatcgagat gtctttggct
     4921 actacgtaga caagggctgg atgtgtgtgc aggttttctt tgttcggcag gggaaactga
     4981 ttgagcggaa tgtcaatctt tttccatact ataatgatgc gggcgaagac tttttgacct
     5041 acatcggtca gttttacagg gaccagcaac atttgattcc ctcagaggtc ttgataccgc
     5101 aggatattga ccaagaagcc gtagaagctc tggtagatac caaggtcatc aagccccagc
     5161 gtggcgagaa gaagcaactc atcaatctgg caaccaagaa tgcgcgtgtc agtctggagc
     5221 agaagtttaa tcttttggag aaaaatctgg agaagaccta cggtgcggtg gagaatatcg
     5281 gtcagctttt ggggattccg acgcctgtgc ggattgaggc tttcgataac tccaatatta
     5341 tggggactag tcctgtgtcg gctatggttg tctttgaaaa tggggtgcct aataaaaaag
     5401 aataccgcaa gtacaagatt aatacggttg agggaccgga tgactatgct tccatgcggg
     5461 aagttttgca gcggcggtac agtcgggtgc tgcgggacgg tttgacaccg cctgatttga
     5521 ttatcattga cggtggtcag gggcaggtca atgtcgccaa gcaggtcatc gagcaggagt
     5581 tgggtatgtc cattcccatt gcaggtttgc agaagaatga caagcaccag acacacgaat
     5641 tgctctttgg taatccgctg gaagtagttg agttgcctcg caattcacag gaatttttcc
     5701 tcctgcatcg gattcaagat gaggttcacc gctttgctat tgagtttcat cgtcaggttc
     5761 ggtcaaaaaa ttccttctca tccaaattgg acggcataga aggcttggga ccaaaacgca
     5821 agcagacttt gatgaagcat ttcaagtcta ttggcaatat ccagtcggcc agtctacaag
     5881 aaatcgcaga agcaggagtg ccgtataaag tagcggaaaa ggtcaaggaa agcctttctt
     5941 caaaggatga gatttgaaaa cattcccata atgtgttaga ataaactatc aatgactaaa
     6001 taaggagaaa gtaatggctt ttggtgaaga aaaacacaaa aaaacaacat ttgagaaagt
     6061 gactttagta atcgttgtcg tgatggttgt agcgacattg gctggcttga ttttaccagc
     6121 tattaatgca ttgatgaatt aagaacctgt attcactgtt cagcacttct atactaaggc
     6181 tagtttttaa gcactcatcg ttagtttttt tcaaaataca gtcagtattt tgaaaaaacc
     6241 accttgattg gctaaaaacg atttcttata tataagcact tcgcttgtga atacaggtct
     6301 aaaacgcttt taagcgtttt tttctaggaa agaatagggt aaattatgag tatgttttta
     6361 gatactgcca agattaaggt caaggcaggg aaaggtggcg acgggatggt cgccttccgc
     6421 cgtgaaaagt atgtacctaa tggcggtcct tggggcggtg atggtggtca tggtggaaat
     6481 gttgtcttcg ttgtagatga aggcttgcgg accttgatgg acttccgtta caatcgtcgc
     6541 tttaaggctg atgatggtga aaaagggatg accaaaggta tgcacggtcg aggagcggaa
     6601 gatttaattg ttcgtgtacc gcagggaaca actgtgcgtg acgctgatac aggtaaaatc
     6661 attacagacc tggttgagaa tgggcaggaa tttgtcattg cccacggtgg tcgtggtggt
     6721 cgtggaaata ttcgttttgc gacacctaag aatccagcgc ctgagatttc tgaaaatgga
     6781 gaaccaggtg aggagcgcaa tcttgaatta gaattgaaag tgttggctga tgtaggcctt
     6841 gtaggtttcc catcagttgg aaaatcaacg cttctcagtg ttattacagc agctaaacct
     6901 aaaattggtg cctaccattt tacaaccatt gtgccaaatt tgggaatggt tcggaccaag
     6961 tctggtgagt catttgcagt ggctgatttg cctggcttga ttgaaggtgc tagtcaaggt
     7021 gttggattgg gaacgcaatt cctacgtcat attgagcgga cacgtgttat cttgcatgtt
     7081 ttggatatgt cagcaagtga aggtcgtgat ccttacgagg actatgtagc catcaacaat
     7141 gaattggaaa cctataatct tcgtttaatg gagcgccctc aaattattgt tgccaacaag
     7201 atggacatgc cagaggcaga agaacatttg gaagagttca agaaaaaatt ggcaaccaac
     7261 tatgatgagt ttgaagaatt gccacagatt ttcccaattt ctggtattgc tcaccaaggt
     7321 ctggaaaatc tactagaagc tacagctgag ttgcttgaga aaacgccaga attcttgctc
     7381 tacgacgaat cagatttcca agaagaagag gcttactatg gctttaaccc agatgaacca
     7441 gaatttgcaa tcagtcgtgc agacgatgca agttggattc tatctggtga caagctggaa
     7501 aagctcttca ccatgaccaa cttcgatcgc gacgaatctg tcatgaaatt tgctcgtcag
     7561 ttacgtggaa tgggtgtcga tgaagccctc cgtgcgcgtg gtgccaaaga cggcgacatc
     7621 gtccgtattg gcaagttcga atttgagttc gttgattaac gtagcagttt gactgcaagt
     7681 taacttgcta gttaaactac gccaagtcct atgggatctt ggctagctac cttactaact
     7741 tcacccaaat ggtggtatca accatttggg ttccgtgtcg taactttgga atcacatcaa
     7801 aatcctaaag gacttgatgt gatacggtag ccgctatggc ggactaccta ccaaccttac
     7861 taattccgca caggcagtgg tatcgactgc ctgtactcca tatcgtaacc tgtccggtaa
     7921 gttagtctag tatgaactta gaaattcgag agcagactat agctttacaa atttcgttca
     7981 gttggtgaca gtcgtttgtg agcaatctta aaaaaaatag ttggaggtct gtatgggtga
     8041 taagccaata tccttcaagg ataagtatgg aaattttgtc tcagcagccg atgtttggaa
     8101 tgctgaaaaa ttagaagaac tctttaacag gctcaatccc aatcgcaaac tccgtttgga
     8161 acgtgagcgt ttggcaagag agcaagaaaa ggaataacgt caaaatctgc ttgttgtatc
     8221 agaaaacagg cagatttttt taaaatttat tgactattta ttattggtta tatgtataag
     8281 aatacaagat atggagctaa tatttttgcg gtgtcggtaa ttttatagaa attctcaaaa
     8341 gtgaaatttc ttaaaaaata tgtaaaaact atgaatatac tggtttcttc ttgaattgaa
     8401 gcgaattcat aatgtttttt tgaggaattt tataatatta cttggcattt aaagttattt
     8461 gtagtataat acctcgaatg attgcgggag ttttcaaggc tttgatacaa agagtagaaa
     8521 atttgtgtaa ttaaattaat atttatatgg gggatttttt atgcgtagat caaataaaaa
     8581 atcatttgac tggtacggta cgaaacaaca attttcgatt cgtaagtatc attttggggc
     8641 agcaagcgtt ttgcttggtg tgtcgttagt tttaggtgct ggtgcacagg ttgttaaggc
     8701 tgatgaaact gttgcttcat cagaaccaac tattgccagt agtgtagcgc ctgcttcaac
     8761 agaagcggtt gcagaagaag cagaaaaaac aaatgctgaa aatacgagtg cagtagctac
     8821 gacttcaaca gaagttgaaa aagcgaaagc tgttcttgaa caggtaacat cagaatcacc
     8881 acttttggct ggtcttggtc aaaaagagtt ggctaaaact gaagatgcaa ctcttgcaaa
     8941 agctatagag gatgctcaaa caaaacttgc agcagctaag gcaattttgg ctgactcaga
     9001 agcaactgtt gagcaagttg aagcgcaagt cgcagcggtt aaagtagcca acgaggcgct
     9061 agggaatgaa ttgcaaaaat acactgtaga tggtctcttg acagcggctc ttgatacagt
     9121 agcacctgat acaactgcat caacattgaa agttggtgat ggcgaaggta cccttctaga
     9181 tagcactaca acagcaacgc cttcaatggc tgagccaaat ggtgcagcaa ttgctccaca
     9241 tacacttcga actcaagatg gaattaaagc gacatcagag ccaaattggt atacttttga
     9301 atcgtacgat ttgtactcat ataataaaaa tatggctagc tcaacttata aaggagctga
     9361 agttgatgcc tacattcgtt actctttgga taatgattcg tcaacaactg ctgttttagc
     9421 agagttggta agtaggacaa ctggtgatgt gttagagaaa tatacgattg aaccgggcga
     9481 gagtgttacg ttttcacatc cgacaaaagt taatgctaat aatagcaata taactgtgac
     9541 ttatgatacc tcattagctt ctgctaatac tcctggagca ttgaaattct ctgctaatga
     9601 tgatgtttat tcaacaatta ttgtacctgc ttatcagatt aatacaactc gttacgtcac
     9661 tgaaagtggc aaagttttgg caacctatgg tcttcaaact attgcaggac aggtagttac
     9721 tccatcttct gttcgtgtat ttactgggta tgattatgtg gcaactacaa ctaaagccgt
     9781 tcaaggtcca tatccaaagg gaacggtata ccttgctggt acggttcaaa aggatacagt
     9841 acaatataaa gttattcgtg aaattgtgga gaacgaccaa gcagttctta aattctatta
     9901 tttagatcct acctataagg gtgaagtaga ttggagagga actgatacga ctgggtttat
     9961 tgagttgctt acaacttccc caacaaccta taaagttggt actatatacg attacaatat
    10021 taattcaaaa attacagctc catttactat tgatcctacc aagaatgtta tggttttcaa
    10081 ggaaagtgaa cagaacgagc aaggtagcaa atatcgcgtc attgctcaat ggtcaggaga
    10141 tgaaaccact aaaggtatat atggaaaaat ctatatcgct actcaggttt ggacgactaa
    10201 attgggaaca aacgagtggg gatggtttga ctattctgat gaccaagctg gtataaaatt
    10261 taataacaaa ggtttttggc cggcaggtgt tcaaaataca cttcgaaatg ctactccagc
    10321 tacagctgta gagactactt atatctacaa agaaagttcc aagtatggtg atgtcattgt
    10381 tgagtactac gatactgacg gaaaacaaat tgtaaattca gttgtagata ctcctaagtc
    10441 agctcttggc acagagtata atacagatgt ggaccgtaga ccagccagct tggttgctgc
    10501 tgatgggaca gtctacttct acaaagaagt taagtctgat tcagctaaga caaccggtac
    10561 agtagttgca ggtacgacaa ctgttaagta tgtttacgaa aaagctggta gcgttaatgt
    10621 taacttcgtt gacatcaatg gtaaagtaat caaagctcct gtttcagatg aaaaagatgc
    10681 gaaacctggt tacaattatg ataccgactt ggatcagaaa ttagcttcca tcacttttga
    10741 aggcaaggaa tacaaacttg ttcctgctgg tgattatccg gttggtaaag ttggcaaggg
    10801 aaataacttg attgaagttg gtaataatac tgcgaaaggt attgacccaa caacaggcaa
    10861 aattgaagcc ggtgttaaca aagaagttac ctatgtctat agagcagtga caggttctgt
    10921 agttgtaaat tacaaagata cagaaggtaa tgtgattaaa gatccagaaa cggatgtgtc
    10981 tgatgcaccg gttggagatg cttatactac aactgacaag aaaccaaacg aaatcatcac
    11041 aaaagatgga tcacgctatg ttcttgttcc atctaagaca gatggtgagg aaaatggtaa
    11101 agttatcgaa ggaacaatca cagtaactta tgtttaccag aaagttgcaa actggattcc
    11161 agagattcca aatgtaccag aaacagaccg tccaaaagta ccttacccat ttgacccaac
    11221 agagccagac gagccaatcg atccaacgac accaggaaca aatggcgagg ttccaaatat
    11281 tccttacgtt ccaggatata caccggttga tcctaaggat aacacgccgt tgaaaccaat
    11341 tgatccaaat gatccaggta agggttatgt accaccaaca ccagaaaatc caggtgttga
    11401 tacaccaatt ccttatgttc cagttaaaaa agtcgtaact aaccacgttg atgaagaggg
    11461 taaccctatt gcaccgcaag aagagggaac aaaaccaaac aaatcaatcc caggttacga
    11521 gttcacaggt aaaactgtta ctgacgaaga tggcaacaca actcacatct acaagaaaac
    11581 accagaagtt aagaatggta cagttgttgt taactatgta acagaagatg gcacagttat
    11641 caaggaacct gtaacagata caccaacttc tccagaaggc acaccatacg acactacaga
    11701 caacaaacct aagacaatca ctttcaaagg tgaagagtat gaattggttc gtgttgacgg
    11761 tacagaaaac ggtaaagttg tagaaggtga aacagttgtg acttacgttt accgtaaagt
    11821 cgaaacacct gctaagaaag ttgtaactaa ccacgttgat gaagagggta accctgttgc
    11881 gccgcaagaa gagggaacaa aaccaaacaa atcaatccca ggttacgaat ttacaggtaa
    11941 aactgttact gacgaagatg gcaacacaac tcacatctac aagaaaacac ctgctaagaa
    12001 agttgtgact aaccacgttg atgaagaagg taaccctatt gctccacaag aggatgggac
    12061 aacaccaaaa cgtcaaattt caggttacga gtatgtgcgt actgtagttg atgaagaagg
    12121 taacacgaca catatttatc gcaaactttc taataaacca acaacacctg agaaggaaac
    12181 tcctgcaaaa cctcaagcag gtaaaaccgc ttcaggtaaa gctcaattgc caaatactgg
    12241 tgaggcttca tctgtggcag gtgcgcttgg tacagcaatg cttgtcgcaa cacttgcgtt
    12301 tgcaagaaaa cgtcgtcgta acgaagatta gtcaaaattc tttatacaga ctttattccc
    12361 ccacatagaa agtataagaa ttgtacgtaa catgcaggat tgcctttccg aaaaaatgag
    12421 gctgggcaaa aagtccagag ttacatctta gagttcgctc catttccaac ctccaacagt
    12481 cactactctg actgttggag ctgtgtgggg gtgggagacg agctcttttt ttattcggtc
    12541 gagttctttc tcgctcccat ttttttgtct tattttgata tactagatat agtatattca
    12601 acgaaagcat gagaatttga tatgaaatta gtctatacag atattcgcaa tcctctgacg
    12661 cagtatttga cagaacagac agcaacattt gccgagcagg gcaaacgtgt tttttatatt
    12721 gctccaaact ctctatcctt tgagatggaa cgcaaggtct tggaatacct gcctgaacag
    12781 gcaacttttg atattatcgt gacccgtttt gggcaattgg cccgctattt gatgattgat
    12841 aggaaagaag caggtcaacc gctggatgat gttggtctgg caatgatttt ctttcgtgtt
    12901 ctatcgcaat ttgaagatgg cgacttgaaa gtctatggtc gtttacagac agattttggt
    12961 ttcattaacc agctagttgc tttatacaaa gaattgcaac gggcaaatat gtctattttg
    13021 gacttggaag ccatggagtc accagataag caggctgatt tggtaaagat ttttctggct
    13081 gtaacagaca ttttgtccaa agaaggcttt gagcatcagt ctaagttagc acagttgact
    13141 ggcttggtag aaactggtca gttggatgag cagttgaaga acattgtgct ggtggtagac
    13201 ggattttctc gtttttcggc agaagaagaa gctttagtaa gtgctttaaa tgaacgggtg
    13261 tcggaaatcc tgattggtgt atatgccagc aagaaagctg tgcaagccac ctatgcggaa
    13321 ggcaacgtct atcaagccaa cgttgacttt ttacgtcagt tatcagcgca gttccagact
    13381 aaggcaactt atattggtca agagccggtc ttggacagta tcggtaaatt ttcaaagaat
    13441 atggaagcct attatgacta cagcgggacc atgatagact tgactccagc agaccaagaa
    13501 aaaatccaac tctgggaggt tgttaaccaa aaagaagaag tggagcaagt tgcgactgca
    13561 attcgtcagc atgtgcatca aggtgctcgc tataaagaca tcttgttgct cttgggtgac
    13621 gtggatagct acaagttgca gattggcaag atttttgaca agtatgacat tccttactat
    13681 ttcggtaagg cagaggagat gagtcatcat ccgttggtcc attttgtgga gtccttggag
    13741 cgcttgcgtc gctatcgttt tcgtgcggaa gacctgctca atctcctcaa atctggtttg
    13801 tacgccagca tttcccaaaa ggagctggac ctatttgagt cttacatcct ttttgcggat
    13861 atgaaggggc aggctgcttt ttcgagggct ttttcggtca atggcagagc agactatgat
    13921 gctgaagtca tcaaggagaa aaggcttgtc tatgatttga ctgtcttgga acccctacgt
    13981 gctaagatta tggaaccttt gaaccagctg tttaaggctg gaccacaatc tggaactgcc
    14041 ttgcttgaga aatttatggc atttttggag gcaattgatc ttcctaaaaa tatggaaaaa
    14101 atgtctcgaa atttgagtga agtagagcaa gaaaaagaag agcaggtctg gaaaagtttt
    14161 acccatcttc ttgagaattt tcatcagatt ttcggaaaag aaaagttgaa aatggatgat
    14221 tttctggcta ttttacaggc tggaatgcag gccagccact atcgtaccgt tcctgcaaca
    14281 gttgatgtgg tcaatgtcaa atcctatgat ttgatcgagc cgcacactgc aaagtatgtt
    14341 tatgctatcg gaatgggtca gtcaaatttt ccaaaagttg ccaagaatac cagtctattg
    14401 acagaagaag agatggaaaa agtcaatctt gtctcggcta gttcctcacg tttcgatttg
    14461 gtcagccgag agaatatcaa gaagaaccat gctgctatga tgtctctgct taattctgcg
    14521 acggaacagt tagtcatttc tactcctcag atttataacg aaggcgaaga tagtctctcc
    14581 ccatatatca agattctcca aaaaatgggg ttgaaatccg aagaacgtgg tcggattaaa
    14641 acacttagtc cacaagacat cggtcactac aagagtctct tatcccgttt gattgagtcg
    14701 gaaagaccaa gccttgaaac agaggagtgg gaagggcaga gggccttctg gacagtcttg
    14761 gttcgccatt tgaagaagaa attggagtcg caaagcattg aaattccgac cattactggt
    14821 gatattgctt ctaaacaact ttcagatgaa accttggctg ctttatatcc agaggataag
    14881 ccgctcaatc tttctgcatc cagcttgacc aatttctaca ataaccaata tctttacttt
    14941 gtccgtaatg ttcttcgcct gcgggaacag gaatccatcc atccgactgc ctttcagcat
    15001 ggtttgttct tgcatcggat ttttgaacga gtggtcatgg accaatctga gctagatttt
    15061 gatcaaaaag tggacaaggc aattctgcgt acgcgtgacg aggcagaatt tgctatgttt
    15121 tataaccagg atgcggatgc tcgttatacg gaggaggtgc tggataagat tgctcgttcc
    15181 agtgcgacta ttttgcggga taatgatttg gttgaaattg atggccagga aaagagtttc
    15241 cgtcaggata aggcgctggt ttttgacctt caaaatggca agagtgtgca tgtaaatggt
    15301 accattgacc gcttggatac cttacaaatc aatcaggctg tcggtgttgt ggattacaag
    15361 tccagcgacc aatctttttc agttggggat ttttataatg gtctcaaacc tcagttggtg
    15421 acctacttgg cagccttaca agagctggat gaaaccaagg ataaacctgt ttttggggct
    15481 atgtatttgc atttgcagga tccgattatc aaattaaaag acacgaagaa tttagaacag
    15541 ctagaaggag ctgccaatac tagcttggtt tacaaggggc ttttcttgaa ggaggaaagt
    15601 ctggggctca atcattttta tcagactcgt aaccaactct acacggaaga tgagtttgcg
    15661 gttttattga accacaacca agaactatac aaacaagctg ccatggacat tctggctggt
    15721 cgctttgcta ttaaccctta taccaaagat gggcgttcgg tggcaggtga gcagctgaag
    15781 gcaatcactg gttttgaggc ggatcggcac atgggcatgg cacggcggtt ggtcaaagag
    15841 gccaagcgtc aagattggat ggaacgaatg aaaggaggtc aggactaatg gcttttgaac
    15901 aatttttaag cgcggaagaa atcaaggctg tgcagctggc ggaagcccat tctgataagc
    15961 agcaaaaacg cacagctgag cagattgaag ctatctacac gcacggtcag aatgttctgg
    16021 tctctgcatc ggctgggtca ggaaagactt tcgtcatggt tcaacggatt ttggataagt
    16081 tgaagcgtgg tatagggatt gaccagctct ttatctcgac ctttacagtc aaggcggctg
    16141 gtgaattgaa ggagcggatt gagaaaaagc tcaatgaaac tattgcggag acgaccgata
    16201 tggaattgcg tcggcatttg tcggcccagt tggcagattt gactaaggcg gatattggga
    16261 ccatggactc ctttacgcag aaattggtga ccacctatgg ttatagcctt ggtatttctc
    16321 cgcagtttcg gattttacag gatgagactg aaaaagcgag tctgaaaaaa gaggtttttg
    16381 accagctctt tgcagattat cttgaagagg atgaaaatgg tgcattccgc aaactggttc
    16441 gcaatttttc gggcaaccgc aaggataata gtggtttccg tcaggttgtt tatcaggtgc
    16501 atgattttag ccaatcgacg agcagtccaa ctaaatggct gaaagaacag gctgttcaag
    16561 ctgatttgta cagtcaagag cgtatagagc agatgctaga gcaaggcttt aaggaaaaag
    16621 tgttagacaa gctctatcaa gctgcagatt tcttccgcta ccatgtggaa tggggcagaa
    16681 atgactttgg gtctgctaaa tattttgcaa atgtggaaga agtcttggat ttgttgacag
    16741 gtttagatag tctggaccag aaggacttga tggaacgggt tgaaagaatt cttctcatca
    16801 ataatcagtc cagaggcaag ggcctgacca atgccaatcg accaaaagat gagcatttga
    16861 tagcctttaa agaagaatac aatgctggta agagccagat tatctcagaa ctacgagatt
    16921 tgggtcagga agtttatgaa ttgaccctgc tgaaagacta tcaggttcaa gctttgccac
    16981 tactgatatt attacgtgac tttgtcttgg atttttcgca ggcttatctg gatgtcaaaa
    17041 tcaaggaagc agcctttgaa tttggcgaca ttgggcattt cgctattcgt attttagaag
    17101 aaaatgcgga cattcgccag tttttccaag agaaatatca cgaagtcatg gtggacgagt
    17161 accaggataa caaccatagc caagagcgaa tgctggacct gctgtctaac ggtcacaacc
    17221 gctttatggt gggtgatatt aagcagtcca tctatcgatt ccgtcaggcg gatccgatga
    17281 ttttccaaga gaaatttgaa ctgtatcagg caaatcctca gtctgggaaa ttgattttgc
    17341 tcaaggaaaa tttccgtagt cagattgaag tcttggaggc aacaaatgct attttcacgc
    17401 gcctgatgga ccgtcaagta ggggaaatca agtatgacga cacccacagt ctagtggctg
    17461 gtagtcctgg tcaaaaaatt gcccagccca agaatgagat ggaatacttg atttacgatc
    17521 agcaggatag cgcaaactct tcaacagatg ctgaggaaga aacacctctg actgctggtg
    17581 aaattgaggt cgttgcaaaa gagattattc gtcttcacaa tgaagaagga gcagacttca
    17641 aggacatcac tctcttggta caaaagcgga cgcacaatga cctcattatg tccatttttg
    17701 aaaaacatgg gattccaatt gtggcagatg gcggagcagc ctcctatctg caatctttag
    17761 aagtcatgat catgctggat accttgcggg ttatcaataa tccactcaat gactatgcct
    17821 tggttgccct gcttaaatca cctatgttcc gattcgatga agatgaactg acacggattt
    17881 ccttgcaggc cgggacaggt ttcttttacc agaaaatgga aatagcccag caagcaagtg
    17941 ggcaacatcc tgaactgatg tcagaaaagt tgaagaagaa aatcacggat ttcttgtcta
    18001 ttttggaaaa ttggcgtgcc tatgccaaac tgcattctat ttatgacatg atttggaaga
    18061 tgttcaatga aaaattctac tatgactatg tcggtgcgct tccaaatggc agcaagcgtc
    18121 aggccaatct atacgcactt ggtctgcgtg ccaaccagtt tgaaaaaaca ggctacaagg
    18181 gattgtctcg cttcatcgcc atgattgatc gtgccttagc aaatgacaag gacttggcag
    18241 atgtacaaga gtttctaccg caaaatgctg tacagcttat gactattcac aagtcaaaag
    18301 gtttggagtt caagtatgtc ttcctcatga acatcgataa gcgatttaac ttggaagacc
    18361 attaccaatc agtcatcatc agccggaaaa atggtctagg tatccaatat ttagcagata
    18421 tgaaagataa ggtaaatagc ccattacctc aggtgcgagt cttaatgaac accctccctt
    18481 atcaaaataa tctccaagag ctaaaaattg ccaatctttc ggaacaaatg cggttgcttt
    18541 atgttgcact aacacgtgct gagaagaagc tgtatctggt tggaaagggt aatgctgaca
    18601 agttagctga aaaatacgat ggcaaaaagg aaaatggcgt actagcccaa tcaactcgtg
    18661 aaagtatggc gaccttccaa gactggattc tagccattga tgaggccttc tcaggagaag
    18721 acttgcactt caagaaagtc tttgtgacgg atgaggattt gacagaggag aagattggta
    18781 aacttacatt gaagagtaag ctagaagacg ccagccttaa agatattcgt caatcagaag
    18841 acatcgccca ggccttggac cagttgtctt ctgtccaaga gttgaacgaa cgctataagg
    18901 ctgctataga attgccaagt ttacggactc caagtcaaat caagaagctc tatgagccta
    18961 ttttggaaca agaaggcatg gaagttatgg aaaaatacca gcccaaacgg accttcaact
    19021 tgccagattt ctccaagaaa ccaaaaatca ctggagccca agtcggttca gcagtgcatg
    19081 aactcatgca acgcttggat ctgtcttggt tggtgacaga agatacggta agagcagccc
    19141 tagaagctgt tcatgctgag caagcgatta aagataaaat caatgttcag atgattttgg
    19201 atttctttga tacagactta ggaagagaaa ttctagccaa tacggacaaa ctccaccgag
    19261 aagctccatt tgcaagtttg cagacagatt ctgtatctca ggaaaacttt gtccttcgtg
    19321 ggattatcga tggctaccta ctgtatgatg atcatattgt cctctttgat tacaagacag
    19381 acaaatatga ccaaccaatc caactcagcc aacgttacca agctcaaatg caactctatg
    19441 cggaggcatt gaaaaaagcc tacaagatag atcgtgtaga ctgccatttg attttgcttg
    19501 gtggggagag aatagaagtg gtcgaagtca acatatagtc atttagtttt tcttttatta
    19561 cttcgttaac tcgctttgcc gtactccagt actgtctgca gctcgttgcc tagtactaaa
    19621 agtaaactaa aagactataa ataaattaaa agcgagtggt taaactcgct ttttctgttt
    19681 ggttagattg agtccgaatg ggacaaggtt ctcttctttt ttcaaaatcc gctggatatt
    19741 ttccttgtga cggatgatga caaaactgcc aagaaagacg ataatcactg taaagagcca
    19801 gtcgtagctt ggcaggatga ttcctattgc tggaaatagc agagctccta agattgctaa
    19861 agctgctgct aagacgctag agaaggaaac catggacgtc aagtagagtg aactggcaaa
    19921 gattagaacg agatagaggc aaaaagcagg tgcgacgccc agaagcatac cagcagatgt
    19981 tgcaaccgcc tttccacctt tgaattgggc aaagataggg aaagtgtgtc ccagtacagc
    20041 cattaaccca aatacaatcg gagaaattcc ttgggcatga aagatcagcg gaagcaaggc
    20101 tgccaacgtt cccttgagaa aatcaattac aaatacaatc gtaccagcct ttggacctaa
    20161 aatacggaat gtgttggttg ttccagtatt tcccgaacca tgctcacgaa tgttgatatt
    20221 gaaaaaagtt tgacctatcc aaagtcctga cgggatagat cccaacaaat atgcaataaa
    20281 tagtaaaata agattgacta acatgttttc attatagcat attttcatct aatttagtcc
    20341 aaaatcttat tttgtgagct gtctcagccg ataggtaaca gaaatatttt gcaaaaatgc
    20401 taaaaaactt gtaatataga aaggatgata agtatggagg tcagttttgg ctaagaaaga
    20461 gattgatata aataattaca atgacgatgc cattcaggta ttggaagggc tagatgccgt
    20521 tcgcaaacgc cctggtatgt acatcggctc cacagacgga aatggcttgc accacatggt
    20581 ctgggagatt gtcgataacg ctgtcgatga agccctgtct ggtttcggtg accgaattga
    20641 cgtgactatt aacaaggacg gtagtctgtc cgtttccgac cgtggacgcg ggatgcccgt
    20701 tggtatgcat gcaacaggca agccaacagt tgaagtcatc ttcaccgttc tccacgcagg
    20761 tggtaagttc ggtcaaggtg gctacaagac ttcaggtggt ctccacgggg ttggttcttc
    20821 ggtggtcaat gccctgtcca gctggttgga agttgagatt acccgtgacg gagctgttta
    20881 caagcaacgg tttgagcagg gcggaaagcc agtcacgacc ctagagaaga ttggtaccgc
    20941 tccaaaatca aaaacaggta caaaagttac ctttatgcct gacgatacca tcttctcaac
    21001 gacggatttc aagttcaaca ccattgccga acgcctgaaa gaatcagcct tcttgctcaa
    21061 gcaagtcacc atgactctga cagatgagcg aacaggtgag caggaggagt atcactatga
    21121 aaatggcgtt caggactttg tatcctacct caacgaagac aaggaaactc tgacacctgt
    21181 cctttatttt gaaggagaag atgctggttt ccaagtccaa gttgctatgc agtacaacga
    21241 cggctattca gacaatatcc tgtcttttgt caacaacgtt cggaccaagg acggcggtac
    21301 ccacgaaaca ggtctcaaac tagccattac caaggccatg aacgactatg ctcgtaagac
    21361 caacttactc aaggaaaagg acaagaactt ggaaggctct gactaccgtg aaggtttgtc
    21421 tgcggtcctc tctatccttg tgccagaaga gcatttgcag tttgaaggac agaccaagga
    21481 caagctgggt agcccgcttg ctcgccctgt ggtggacggc attgtgtcgg acaagctgac
    21541 cttcttcctt ttggaaaatg gcgagctagc atctaatcta gttcgtaaag ccattaaggc
    21601 gcgtgacgcc cgagaggctg cgcgtaaggc gcgtgacgaa tcccgaaacg gtaagaaaaa
    21661 caagaaggat aagggccttt tgtcagggaa attaacccca gcccagtcca aaaatccagc
    21721 caaaaatgaa ctctatctgg tcgagggaga ttcggccgga ggttctgcca aacaaggccg
    21781 tgaccgcaag tttcaagcca ttctaccttt gcgtggtaag gttatcaata ccgccaaggc
    21841 aaaaatggcg gacatcctca aaaacgaaga aatcaatacc atgatttaca ccattggtgc
    21901 aggtgttggg tcggatttca ctatcgaaga tgtcaactat gacaagatta ttatcatgac
    21961 cgatgcggac acggacggtg ctcacattca gaccctcttg ctgaccttct tctatcgcta
    22021 tatgcgaccg ctagtagagg caggccgggt ttatatcgcc cttccgccgc tctacaagat
    22081 gtccaaaggc aagggaaagg cagaaaagat tgcttatgct tggtctgatg gagaattaga
    22141 agatctccgc aaggattttg gcaagggctt tatcctccag cgctacaagg ggcttggtga
    22201 gatgaatgcc gatcagctct gggaaacgac catgaaccct gaaacccgta ccctcatccg
    22261 tgtcaccatt gaagacctag cccgtgctga acgccgtgtg tctgtcctca tgggcgacaa
    22321 ggtcgagcca cgtcgcaagt ggattgaaga caatgtgaag tttaccttgg aagagagtac
    22381 ggtgttttag aaattgaaat acaattgtaa gactacgtta tgaaattaca aatttggaat
    22441 gaatcttata gtctccaatg gaagggaacc tattttcttg ctttgtctga ctatcctaat
    22501 attcaagatt gggaattaga gaaaattgta gcttttttag cttatgagaa actttacggc
    22561 agagagacgc taatagattg tgaagacaaa gtaatgcttg aacaacttgt ctatttatct
    22621 tgttgctcac ccactgcatt tccatttact ccttcgaaaa agattgtggc ctcaacatat
    22681 gatgttggtg gtaattacgt ttattcggat tttttaagtc atacgtgtac tgtcgaaact
    22741 gcacaagaga ttttcatgag tggtcaatta ttgtccgctg ttcgtgcgtt caacaaaact
    22801 catcaagaac tcgttcagga taatcgaaac gcagcaggtg atccagaaga ttattttgat
    22861 tatgtcatgc taggttggtc aaatacaagt tctggctaca gacttgcaat ggaaagatta
    22921 ctaggtagag aaccaacaga ggaagagttg gggaattctt ttattccagg cgtaagtttt
    22981 cattttaagt acgacgatat agtcaatgca gatggttatg tttttgatgg ataccaccct
    23041 gcaaaggtca gggattgttt aagtttgaaa tatttgtttg cctgtattgt tccttcaagt
    23101 gaaaaatctc attttgagac aattattccc ctagatatgt cttcaaaagt ttattattta
    23161 gactatggtc aggatgggat ggcagattgg tcccaaaagg tctatcaatt tgttgaaaat
    23221 atagcaaact aatactataa actattcaat tcgatgattt gtgataggaa tcgattgttc
    23281 ctaacgccgt gtatctgtct ttatgggcga caaggtcgag ccacgccgca agttgattga
    23341 agacaatgtt aagtttacct tggaggaatc gacagctttt actaaataat ctcagggaag
    23401 ttgaggtgcc atatggtttt agaaaataaa ttggagattg aaaattcggc tgaattagcc
    23461 cgtcttgaag aacaaatcag caagaaaaaa gcagctcaac tgttcgaaaa tggtcagtta
    23521 ttccagatag aagttggaac ctttgccggt ttggcacaca ttcatcaagc tttatttgag
    23581 gatatttacg actttgcagg taagattcgt gatgtcaaca ttgccaacca aagataattt
    23641 tcaattcgct ccacgtatct ttcttgagca gtctttggcc tatatcgaca agctacctca
    23701 cgaaaccttt gatgaaatcg ttgaaaaata tgcagatatg aacattgcac atccattccg
    23761 agaaggaaat ggacgcagta tgcgtatctg gttggattgt atgctacggg atagtttggg
    23821 tagggttgtt gactggaata gcattgataa agatgaatat ttcaatgcaa tggttagaag
    23881 ccatgtgtca acaggagagc ttaaatatct gttattgcaa gccttaacgg aagatcttgg
    23941 tcaggctact tatttcaagg gcatcgacca ttcttattac tatgagggtt acaacctata
    24001 tcgtattgag gatttgtaaa tctagcataa acaaatgatg tgaggtgttt tatgccacta
    24061 agagtaaaat taactgaccg ttttcaaaaa cggttgtctg aatgggcacg aaagcgcaag
    24121 ccatttaaaa acaaacagga gttggaagag aaatttggcc aagcattgaa gatttggcaa
    24181 tcttccaaaa ctcggcaaca tgcagaaaac ttgctatctg agcaaggaaa attagaacat
    24241 tttttacata gcacggaacg gaaactgtct cgtatgcctt ttggcggaga taagtttgcg
    24301 gctattccag gcttgatttc catggtgcga agctatatcc gtagagatta cagcaagtta
    24361 cccaaagcga caatcctcgc aattataggt gctttgattt attttttcag tcctattgat
    24421 gccttaccag atttcatcct tggag
//
LOCUS       DBNFCK010000018        23349 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.018, whole genome shotgun sequence.
ACCESSION   DBNFCK010000018 DBNFCK010000000
VERSION     DBNFCK010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 23349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23349
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.018"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            1..551
                     /locus_tag="AC1PIW_000564"
                     /pseudo
     CDS             1..551
                     /locus_tag="AC1PIW_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018164076.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            531..740
                     /locus_tag="AC1PIW_000565"
     CDS             531..740
                     /locus_tag="AC1PIW_000565"
                     /EC_number="3.4.26.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HIP6605526.1"
                     /translation="MNPLNISVLITAVLFAFVHSYEISLSFWSALFPGFLFSWIYYKT
                     NHLKYPIVAHILSNSIVTILNVISV"
     gene            804..1478
                     /locus_tag="AC1PIW_000566"
     CDS             804..1478
                     /locus_tag="AC1PIW_000566"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIP6605527.1"
                     /translation="MKIIKSIVLAVGTLLLTLLIQFPAYVGITAFQENVLLKLAVMIG
                     LGLPILALLSVIRRGMMKHQVFSREKLNVLKIVGMTALVFTGQTCLNLFWQYLGLSLS
                     DSNAMDTVNQLNSDNFGVMALSVVLFAPVLEEFLYRGILLEKTAQYFPKHPQMVIVFS
                     ALLFAYCHTWNFSVAFLGHFITGAYLGYLYMCNRRMTDTILAHSFYNASILFLQILFG
                     LVQIGG"
     gene            1576..1851
                     /locus_tag="AC1PIW_000567"
     CDS             1576..1851
                     /locus_tag="AC1PIW_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605528.1"
                     /translation="MRKFFQDLNQEYLETRSSVGEDWLEWIIQRKMTWGMRWGLVGIL
                     MFVLQPLTYNPHFLLGCFYIGFLLWLVYVCGQLFFAARSIFRNLKKR"
     gene            complement(1887..2546)
                     /locus_tag="AC1PIW_000568"
     CDS             complement(1887..2546)
                     /locus_tag="AC1PIW_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HIP6605529.1"
                     /translation="MTPNKEDYLKCIYELGQLDQKITNKLIAEKMAFSAPAVSEMLKK
                     MVAEELISKDAKAGYLLSQTALEMVASLYRKHRLIEVFLVEQLGYSPEEVHEEAEILE
                     HTVSDHFINRLDLLLEQPQTCPHGGSIPQAGQPLIERYQTRLSQLTETGNYQLVRIHD
                     FYQLLQYLEQHELAVGDLLTVTAFDQFAQTITIQYKDKELAVPTAIAQQLFIEKSNRP
                     A"
     gene            2672..3595
                     /locus_tag="AC1PIW_000569"
     CDS             2672..3595
                     /locus_tag="AC1PIW_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter solute-binding protein,
                     Zn/Mn family"
                     /protein_id="HIP6605530.1"
                     /translation="MKKILFSFALLLSLIGLGACRPSQTTEGSSKPRVAVTTSFLNDM
                     VYQLAGDEVERDLLIPAGEDPHLYVAKSSDLSKLQKADLVLYHGLHFEGKMVEALEKT
                     GVAVSKNFNAKDLNTMDEDGEEIVDPHFWFSIPLYKSAVAVASEELQKLLPAKAEMIQ
                     KNTEKYQAQLDDLHAWVEKELSVIPKESRYLVTPHDAFNYFAASYDFTLYAPQGVSTD
                     SEVANSDMIETVNLIIDHNIKAIFTESTTNPERMKKLQEAVKAKGGQVEVVTGEGKEL
                     FSDSLAPEGEEGDTFIDMYKHNVKLMVKYLK"
     gene            4149..4919
                     /locus_tag="AC1PIW_000570"
     CDS             4149..4919
                     /locus_tag="AC1PIW_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HIP6605531.1"
                     /translation="MSAIIELKDVNLAYTASQTMALEDVNLVIPRGSRTAIVGPNGAG
                     KSSLFKVILGLEKPDTGQVRLLGQEAGLERLIAQKVAYIPQSSQVNWQFPATVFEIVL
                     MGRFAHSKGMFRRPTKADRQIVEQALERLKIADLRHRQIDQLSGGQRQRVFLARALAQ
                     EAELYLMDEPLAGIDQATEIMIMDMLKEFQCEGKTSIVIHHDLTTLDAYFDHLVWLHK
                     HVIDSGPMDETLTSENYQATYGIGNGLFLGNAKGGSHV"
     gene            4912..5772
                     /locus_tag="AC1PIW_000571"
     CDS             4912..5772
                     /locus_tag="AC1PIW_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HIP6605532.1"
                     /translation="MFEVFKEYSFWTVALGTVSLAVAASTIGSISVLTKQSLLGDALG
                     HASYPGVIVSFMIFQSRHPLYLLLGAVLSGYLSYALVHWLRRKGGHSLVNALSLVSAS
                     FFGLGMVLKNAIQGNEAFAGASQAGLQTYLFGQAAFIQLDDVILIGIISLLALALFAF
                     FYQDYKLYLFDQTFARVIGVRVKYLQQLTMFLMICLIAVGLKLVGAILMSSFLIAPAV
                     FGLMLGKSYHKSLLLAGIVAIGSAFVGTWISSSVSGLSTGPTIIVCLTGLTLSAFVYI
                     TYVRKENGRV"
     gene            5765..6604
                     /locus_tag="AC1PIW_000572"
     CDS             5765..6604
                     /locus_tag="AC1PIW_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HIP6605533.1"
                     /translation="MFEVLLILMVIASSCGLLGSILVVKNQSMLADALSHSVLLGIVL
                     GFFISHSLDSPLLIVGASLFGLLSVLAIDRLHSRKIAHDAATGLVFSFFFAVAVLLIS
                     LFARNVHLDVDLVLQGEVLFAPLHRMDVLAWSLPVSLVKSSLAWLVIVLFFAWAYHRL
                     QVYLFDSNHARLSGLRTRILEMVILILVSLTTVLAFEAIGSMTVIVFLVAPSMAALCW
                     VKSFWQLLLLGQGIAILTVVLGFLVANQLDLTMSGTCAVVSLLVVCSSIILKNTWSRS
                     SDK"
     gene            6660..8552
                     /locus_tag="AC1PIW_000573"
     CDS             6660..8552
                     /locus_tag="AC1PIW_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloendopeptidase"
                     /protein_id="HIP6605534.1"
                     /translation="MIRLQDDFYEYVNGEWAKTAVIPDDKPRTGGFTDLADEIEKLMI
                     DTTNAWLAGEDVPEDSVLQNFVAFHKQVADYEIRDRLGAEPAQALIAEYKALNSFEEF
                     TSKLAEYELAGKPNLMPFGVAPDFMDATTNVLWADSLGIILPDTTYYEEGHEKGAELL
                     KTWRESQEALLPKFGFSDEEIKDLLDKRLELDATIAKYVLSNEEGSEYAKLYHPYEWA
                     DFTALAPELPLDDFFTAILGQTPDKIIVPEERFWQAAKDIYCADNWELLKATLILKAA
                     GAYTAFLSDEIRILAGAYSRALSGTPQAQNQEKAAYNLAQGYFNQALGLWYAGEKFSP
                     EAKADVEAKVAKMIEVYKSRLETADWLAQETRDKAIVKLNVIKPYIGYPEALPERYYK
                     KLVDPSKSLVENAIELNKIDIAHGWSKWNKPVDIKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDSNGASFDEHGSLNNWWTEEDYAA
                     FEARTQQVIDQFEGQDSYGAKINGKLTVSENIADLGGIAAALEAAKSEDDFSAEEFFT
                     NFARIWRMKARPEFMQMLASVDVHAPGHLRTNIQLPNFDEFHETFGVQEGDGMWRAKE
                     DRVIIW"
     gene            8655..11156
                     /gene="leuS"
                     /locus_tag="AC1PIW_000574"
     CDS             8655..11156
                     /gene="leuS"
                     /locus_tag="AC1PIW_000574"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIP6605535.1"
                     /translation="MSFYNHKEIEPKWQEFWAKNHTFKTGTDAEKPNFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPADFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLEEVDWPESIKD
                     MQRNWIGKSTGANVTFKIKDTDKDFTVFTTRPDTLFGATYAVLAPEHDLVDSITSAEQ
                     AEAVAEYKRQASLKSDLARTDLAKDKTGVWTGAYAINPVNGKEIPIWIADYVLASYGT
                     GAIMAVPAHDERDWEFAKQFGLDIIPVLEGGNVEEAPYTEDGAHINSDFLDGLNKEEA
                     IAKMVAWLEENGVGQEKISYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPENELPLV
                     LPKTSDIKPSGTGESPLANLTDWLEVVREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NDEKLADEDLLKAWLPVDIYIGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDSRGALVATDKVEKRDGSFFHMETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQFGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLLTTKELVAENSGALD
                     KVYNETVKTVTEHIEDLKFNTAIAQLMIFVNAANKEDKLYVDYAKGFVQLIAPFAPHL
                     AEELWQGLANTGQSISYVAWPTYDESKLVESEVEIVVQIKGKVKARLTVAKDLAPAEL
                     EKVALADEKVQAEIAGQTVVKVISVPNKLVNIVVK"
     gene            11331..12692
                     /locus_tag="AC1PIW_000575"
     CDS             11331..12692
                     /locus_tag="AC1PIW_000575"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HIP6605536.1"
                     /translation="MLKTLKLVTIGGGSSYTPELVEGMILRAHQLPIREWWFVDVEQG
                     AKKQEIVVELARRMVAKAGLDWKIESTLDRREALIEADFVTSQFRVGQLPARYLDETI
                     PLSYGMLGQETNGAGGIFKAFRTIEAYKGIIKDMKDLCPNAWLINFTNPAGIVTEAIA
                     NELHWKKVIGLCNIPIGQKKLAAQILGQSEGDLSTRHIGLNHFHFHEVWDKYGENRTQ
                     VILETLYGDLREEMPEAVKNITNLEFPIQLLRTIGMLPCDYHRYYFLEQEMLEDALEQ
                     YRQGNVRAKVVQEVETELFELYKDPQLKDKPKQLELRGGAYYSDVACQIICAIHNDSR
                     EELTVSTVNRGVIPYLPENCVVEVTSIISAQGATPLACPPTPPIVTGYLQLMKQMELV
                     TVQAAMTGDYSLALQAFTLNPLISNGPQAEVLLQDMLLAHENYLPQFAPAIQHIKERR
                     NNQ"
     gene            12689..14089
                     /locus_tag="AC1PIW_000576"
     CDS             12689..14089
                     /locus_tag="AC1PIW_000576"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HIP6605537.1"
                     /translation="MTKDKVFYQFPTNFLWGSSTSGPQSEGIEPGDGKGQNNWDYWYS
                     IEPERFHNKIGPSQTSTFYKHYEKDIDLLVETGHTVFRTSIQWSRLIPTGTGEINQEA
                     VSFYRSVFSLVRAKGIQLFVNLYHFDLPYCLQEKGGWENKEIVRAYEAYARTCFELFD
                     DLVDRWITFNEPIVPVECGYLGNYHYPCKVDAKAAVAVAYHTQLASSLAVKACHEVNP
                     HKKIAIVLNLTPAYPRSEQAEDIKAARIAELFQTKSFLDPSVLGEYPDDLVELIDAYG
                     LMPEVSDEELAIIKENRVDFLGVNYYQPLRVQAPTNSWKEGEPLTPEYFFAPYDMPGK
                     KMNPHRGWEIYEKGIYDIAINIKENYHNIEWFVTENGMGVEGERAFKDNGLIQDDYRI
                     EFIEDHLIELHRAIQEGANCKGYMLWTFIDCWSWLNAYKNRYGLVELDLDTQERIVKK
                     SGKWFKKLSDANGFHR"
     gene            14099..15205
                     /locus_tag="AC1PIW_000577"
     CDS             14099..15205
                     /locus_tag="AC1PIW_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HIP6605538.1"
                     /translation="MVELGFSIYPENYSLEESQAYIKLLHQYGARRLFMSLLQLENGD
                     RTVFELYQKLIDYANQLGMTVIADISPHFIAAQGWDGALMERAAELGLAGIRLDEALP
                     LEEIIEMTHNPRNIKVELNLSTDKILLSNLLASVANRDNIIACHNFYPHAFTGLSEEH
                     FLEMSSFYHQSGIRTAAFVSAQTASEGPWPLSEGLPTLEDHRFWPLPLQIAWMKATGF
                     IDSILISNQFISEEELQSIQTVLNEDTISLMVDIEEGLTPVEREIVAFNHVYRGDISA
                     YVLRSTMPRVVYKDASVPARSDQAIPVGRGDILIDNDLYGRYKGELQIALKEFTISPK
                     VNKVGRISPDYLPLLRFIKPWQEFKLVIASEHFH"
     gene            15297..17291
                     /locus_tag="AC1PIW_000578"
     CDS             15297..17291
                     /locus_tag="AC1PIW_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HIP6605539.1"
                     /translation="MLSKKEVRLLEALLAKQETFVPSIELAKVLSVSDRTVRTYIHQL
                     TSSLENSGAHILSKQGSGYCLQIERPVEFEMFWQEQLLLKKRLTDLTQLEEAGDRQRY
                     ILNKLLFEDPIQSPDCLQRELFIGKTTLHSILADIRKLFIPYQLELLVTRKGIELKGD
                     EPAIRHFIMDYFFDEGNVQSVYGVVETNLLPDIRFTDLMLIVLDECRDAKLRLSDFVM
                     QNLVLHIALLLQRLKIGSGLDRFPIPERIQSSQEYLVAQRIIDRIEETFQVTIPSEEA
                     NYIALHLKVKLTGNPNQDGEVEEIFRQNVENSLLHLSHLTGLELSKDQQLFKGVLAHL
                     MPLRIRLENNIQLGNPLTAEIKDQYFEAFELTKMAFAAIPELMGYSISDDEWAYLTLH
                     VMAAIERYQGFKKTRVLVVCATGIGSALMLKNRLEKEFSSSLEILDTVSYYEVTEERL
                     KDVDLIISSISLSNLIFMTPVINVSVFLSEKDIEAIRSHLQQTTPVIPDQGENVLMEV
                     GQAKQIAEAVFAPHRIIYLEEDCDREVALKQLVTSLDESIEPDFVNRFIEQVNLRESY
                     SPVVFGNRLAFPHPALPMSYSEQIVVGIYKNAVDWGHGKQVQFVFLLSPSKGRNAFLK
                     YISPCLVDFVQDRVLQERLLHHPNYKELIDIFIPLIQRKG"
     gene            17304..17615
                     /locus_tag="AC1PIW_000579"
     CDS             17304..17615
                     /locus_tag="AC1PIW_000579"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIP6605540.1"
                     /translation="MKHIMLVCNAGMSTSMLVTKMQKAAEETGVEATIWAVPVSEADN
                     EVAQKEIDVLLLGPQVKFLLNDYKAKFEPTIKVDAINMMDYGVMNGAKVLEAALKMME
                     G"
     gene            17617..17931
                     /locus_tag="AC1PIW_000580"
     CDS             17617..17931
                     /locus_tag="AC1PIW_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HIP6605541.1"
                     /translation="MNSPEYLEAIMGLIMYGGEAKGFASQAIQAAKEGRIDEAEQLLQ
                     DAIASLNIAHKSQTQLLAKEASGESVELSLLMVHGQDHLMNALTFIDLAKEFVDLYKQ
                     IG"
     gene            17950..18390
                     /locus_tag="AC1PIW_000581"
     CDS             17950..18390
                     /locus_tag="AC1PIW_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HIP6605542.1"
                     /translation="MKISKIGQVPIERLFATIRQSLKDDFQQNTGSILQDQDIIQGLT
                     YTKTFGDKGQHSVKVTVEEFTAPYCYAVCFHSNRGKEHVRYQLEDLGNDQTNIEYSYE
                     MEAGDIFVKGNQFLMEKIFSKSIKRQTNAQLEALIRCSVEPVSV"
     gene            18434..19810
                     /locus_tag="AC1PIW_000582"
     CDS             18434..19810
                     /locus_tag="AC1PIW_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HIP6605543.1"
                     /translation="MSNISEKFMEISGKIGSQRHLIAIRDSFISMMPITMAGTVAVLL
                     NVFLRDIPNNMGWTGFADAMQPVIDINGYVYFGTIGIMALVFAFTFGYNLSLMYKVNP
                     LAGGLISFASFISTIPQSVTISTALDGVDKSVVSALEGLGLSIVTTDGVSALETSQWG
                     AIALKYGGATGLFTALFIGFLATWVYASLVKRNVIIKLPDSVPPAVNKAFAAIIPGTA
                     AIYASSIVAYLAFTLTGQSLGDLVSTYIQLPLLGLSQGLGSVILLTFLVQLFWFFGLH
                     GHNVLAPIMDGVYMVALNENAAAYTATHSTANLPWLWTRGSFDAYAQMGGSGVTLALI
                     IAIFIFSKREEYKMVAKLSAPMGVFNINEPVTFGIPMVLNPLFAIPWLIVPPICATIA
                     YLATAAGIIPPVFAPVPWITPPGLYAYLATGGNIMAALVSLFNLFVAFLIWTPFVIAA
                     NKVKAGEE"
     gene            20142..20753
                     /locus_tag="AC1PIW_000583"
     CDS             20142..20753
                     /locus_tag="AC1PIW_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HIP6605544.1"
                     /translation="MSYQMIKKQFKLGLFTALIGNLLWLGMVNVSADDTVISDSPTVS
                     ALTKKVEAAKIAAKAAAEKIVAEKAAAAAKAALATNMVSEVALEYTANTYPAGQCTWG
                     AKEMAPWVGNYWGNGGDWAASAAALGYEVGTTPKVGAIAVWTDGGYGHVAYVTDVAAN
                     GHIQVKESNYGGVYYPSNVRGFFDPTTTSEGTVSYIYPPAGVN"
     gene            20916..21704
                     /locus_tag="AC1PIW_000584"
     CDS             20916..21704
                     /locus_tag="AC1PIW_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIP6605545.1"
                     /translation="MFTSLLFAGIVLAACNSSAGTSNSTDQTLLAQIKEEGVIQIGTE
                     GAYAPYSYHDESGKLVGYDVEVAEAVAEKLGVKVEFVETEWDSMIAGLDAARFDTIAN
                     QVGITDERKAKYDFSTPYTYIYGALVTQKDNTDITSFADLSGKKSANSLTSNWADLAR
                     ENGAEVVGVDGFSQAVELLNTGRVDATINDNLVYLDYVKQHADAPIKVVTLTDDVSTT
                     AFPVAKGNEDLVKEIDAALAELASEGKLAEISNKYFGEDVSKAK"
     gene            21717..22376
                     /locus_tag="AC1PIW_000585"
     CDS             21717..22376
                     /locus_tag="AC1PIW_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIP6605546.1"
                     /translation="MTERVWQLLLDSFSQILVPGLLVTIPLTILSFTFGLLIAIGTAL
                     VQIAQIPILKQVARFYIWVVRGTPLLVQLYVIFFGLPSLGIVLDAFPSAVLVFSVNTG
                     AYAAETIRASIESVPKGQLEAGYSVGMSFAQTMRRIILPQAFRVAFPPLSNTLIGLVK
                     DTSLAANITVLEMFMATQQIAARTYEPFALYCEVALVYLFFSTILTKLQAYGEKKLAV
                     Y"
     gene            22386..23132
                     /locus_tag="AC1PIW_000586"
     CDS             22386..23132
                     /locus_tag="AC1PIW_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIP6605547.1"
                     /translation="MIQANGVKLTFGGQQVLKGIDVEVNPGEVVVILGPSGSGKTTLL
                     RSLNFLEKADAGHLVFGEDSYDLAQMTKKEIAGIRKRTAFVFQNYNLFANKTALQNVT
                     EGLIVARKVPKQEAERIGKAALDKVGLSDKYGFYPSQLSGGQQQRVGIARAIATNPEV
                     IYFDEPTSALDPELIGEVLAVMMQLAKEGMTMVVVTHEMGFARAVADRVIFMDGGRII
                     EEGRPEDIFDQPKEERTKQFLARIQKVDVE"
BASE COUNT         6690 a         4287 c         5483 g         6889 t
ORIGIN      
        1 acgtcagtaa ggtataaatt cctagaagtt tttggtaaac caaatcaatt attggaatca
       61 attgaggaag cagggaatca tcaatttttc tttttagata ttgaaataaa aggagaagaa
      121 aagaaaggaa tggaaatcgc taaagaaatc cgggctcgag atccttatgc tgttattgtc
      181 tttgtaacaa ctcactcaga atttatgcca gtaacatatc gttatcaggt ttctgcttta
      241 gattttatag ataaaggcct ggaggatagt gactttcaaa aggcagtatc agatgtctta
      301 gtgcatgctt ttgaaaatat tgatcatact gtagctgaaa attcttttgt atacaaaact
      361 gaaactgctc atattcaagt tccttttagt gacatccttt attttgaaaa tcatcaacga
      421 ttcataaagt cattttaaaa acgaaaacag gtcagacaga gttttacggg aaagtatcgg
      481 atatagcgaa agctgatgaa agactttacc aggcacatcg ttcttgtgta gtgaatccat
      541 taaatattag cgtgttaatt acagcagttt tatttgcttt tgtacatagt tatgaaattt
      601 ctttgagttt ctggtcggca ctttttccag gatttttgtt ttcttggatt tactataaaa
      661 cgaaccatct caaatatccg atagtggcac atatcttgtc gaacagtatt gtgaccattt
      721 taaatgttat aagtgtttaa aggtgtgaac attgtgaaaa acaattgagt aggttataaa
      781 tgacaatgga aaggagagag aatatgaaaa taataaaatc catcgtttta gcagtgggta
      841 ccttgctgct cactttgctc attcaatttc cagcttatgt ggggataaca gcctttcaag
      901 agaatgtgct actaaaactt gcagtcatga tagggcttgg tcttccaata ttggcactac
      961 tttctgtcat cagacgagga atgatgaagc atcaagtctt ttcaagagag aagctgaatg
     1021 ttttaaaaat agtcggaatg acagcactgg tatttactgg acaaacttgc ctgaacctct
     1081 tttggcagta tttgggtctt agccttagtg attctaacgc catggataca gtcaatcagt
     1141 taaattcgga caattttggg gtgatggctc tctcagttgt cttgtttgct ccagttttag
     1201 aagaatttct ttacagagga attttgttag aaaagacagc ccagtatttt cctaagcatc
     1261 ctcaaatggt catcgtattt tctgcccttc tatttgctta ttgtcatacc tggaactttt
     1321 cagtggcatt tttaggtcac tttatcacag gggcttatct gggctatctg tatatgtgca
     1381 atagacgaat gacggatact atccttgcac atagcttcta taatgcctcc attttattcc
     1441 tccaaatttt atttggtttg gtgcagattg gtggataaaa attgacagta gacattttgt
     1501 ggaaatgcct gtatactgag aggaagttgg caattttata attgctaact atcaagtgca
     1561 gaggtgggca gaaatatgag aaaatttttt caagacctta atcaagagta tctggaaacc
     1621 agaagttcag ttggagaaga ttggctggag tggattatcc agagaaagat gacctggggt
     1681 atgcggtggg ggttggtagg gattctcatg tttgtcttgc agcctttaac ctataaccct
     1741 cattttctat tgggctgttt ttatataggt ttcctacttt ggttggtgta tgtatgtggg
     1801 caactatttt ttgcagccag atctattttc cgtaatttga aaaagaggta actatttatt
     1861 ttagaagaaa aaatcgctca gcttgtttag gctgggcgat tgcttttttc gatgaataat
     1921 tgttgagcga tggctgttgg gacggcgagc tctttgtcct tgtactggat ggtgatggtc
     1981 tgggcaaact ggtcgaaggc tgtgacggtt agtaaatcac cgacagctaa ttcatgttgt
     2041 tccaagtact gaaggagttg atagaagtca tggatacgga caagctggta gttccctgtc
     2101 tcagttagct gtgacagccg tgtctggtag cgttcgatga gcggttgtcc tgcttgagga
     2161 atgcttcccc cgtgaggaca agtttgaggc tgttccagta gcaggtctag gcgattgata
     2221 aagtgatctg aaacggtgtg ttctaaaatc tcagcctctt catgtacttc ttctggagag
     2281 tagccaagtt gctcaactaa gaatacctca atcaagcggt gtttgcgata gaggctggct
     2341 accatttcaa gggcagtttg actgaggaga taacctgcct tggcatcctt agaaatgagc
     2401 tcttcggcta ccattttttt gagcatttcg gaaacggctg gtgcggagaa ggccatcttc
     2461 tctgcgatga gtttattggt aattttttgg tctaattgac ccagttcata aatacatttt
     2521 aggtaatctt ctttgtttgg tgtcatgaga actcgctttc ttctgaaact attatatcaa
     2581 aaaatttaaa aatcagttga caaaatgatt tttttagatt aatctaatta tatagttagg
     2641 tatgcttaat aaaaataaaa ggagaaccta tatgaagaaa atattgttca gttttgctct
     2701 gctattgtcc ctaatagggt taggagcttg tcgtccatcg caaaccacag aaggctctag
     2761 taagccaaga gtggctgtga ctacttcctt cctcaatgac atggtctatc agttggcggg
     2821 tgatgaggtg gaacgtgatt tgctgattcc agcaggagaa gaccctcatt tgtatgtagc
     2881 caaatctagt gatttatcaa aattacaaaa ggctgacctt gttctctatc acggtttgca
     2941 ctttgaaggt aaaatggttg aagctttgga gaagaccgga gtagctgttt ctaaaaactt
     3001 taatgcaaaa gacttgaata cgatggatga ggatggagaa gagattgtcg atccgcactt
     3061 ctggttttca attcccttgt acaagtcagc tgtagctgta gcttcagagg aactacaaaa
     3121 actcttacct gctaaagctg agatgattca gaaaaataca gaaaaatacc aagcgcagct
     3181 agatgatttg catgcctggg tagaaaaaga attaagtgtg attccaaaag aaagtcgcta
     3241 tttggtaaca ccacatgatg cctttaatta ctttgctgct agttatgact tcactcttta
     3301 tgctcctcaa ggcgtgagca ccgactcaga agtggctaac agtgacatga ttgagacagt
     3361 taacctaatc atcgaccata atatcaaggc tatctttaca gagtctacaa ccaatccaga
     3421 acgcatgaag aaattgcaag aggcagtaaa agccaaaggt ggtcaggttg aagttgttac
     3481 tggcgaaggt aaagagttgt tctcagattc cttggcacca gaaggcgagg aaggggatac
     3541 ctttatcgac atgtacaaac ataatgttaa actgatggtt aaatatctga aatagtacag
     3601 gtcgaacgga cttgctattc aatgaagact agagatatta ccaaagatag agttgatagg
     3661 taccaatatg agggacaatc aattgtttcg agcacaagcg gtatagggct ctttctattg
     3721 gaggtggtaa cgcttggggg aggataggcg ttagtagcaa atcacaccag ttattgaggt
     3781 ccttgactag aaataccgct tgtagacctc gatgatcttg gtttacagcg ggactttata
     3841 tatcagtagt ctgtgcgtga atagagattt caaatgagta gtaaaaagga ctcggtctag
     3901 gtcgagttct ttccgttttt ggaccatttt taacagattt ataaacatac gattgaagga
     3961 agcattggtg ctctgcgaaa atcaaattca tgtactgatg tgttgaaata agcgatgtat
     4021 agggtttgcc agcaatttgt tggtacggta tcttaatctt atcgactttc tcagcttggt
     4081 aggttggtct ggttttgatt ttcattgagt atgaatgagg catatcattt ttaaaaatag
     4141 gaggaaatat gtcagcaatc attgaattaa aagatgtcaa tttagcatat acagccagcc
     4201 agaccatggc tttagaagat gtcaatttgg tgattcccag agggagtcgg acggctattg
     4261 ttggaccaaa tggtgctggg aagtccagtc tattcaaggt cattttgggg ttggaaaaac
     4321 ctgacacagg gcaggttcgc ttgcttggtc aggaggcagg tctagagagg ttgattgctc
     4381 aaaaagtagc ctatattccc caatccagtc aagtaaattg gcaatttcct gcaacagtct
     4441 ttgaaattgt tctgatggga cgcttcgctc atagtaaggg aatgtttaga agaccgacta
     4501 aggctgatag acagattgtt gagcaggctc tagaacggtt aaaaattgcg gatttacgcc
     4561 atcgtcagat tgatcagctt tcaggtgggc agcgtcagcg ggtattttta gcgagagccc
     4621 tggcacagga agcagaattg tatcttatgg atgagcctct agcagggatt gatcaggcaa
     4681 cagaaatcat gatcatggat atgctcaagg agttccaatg tgaagggaaa acttccattg
     4741 tcattcatca tgacttaacg acgctggatg cctactttga tcaccttgtc tggcttcata
     4801 agcatgtgat tgactctggt ccgatggatg agactttgac aagcgagaat tatcaggcga
     4861 cctacggcat aggaaatggg ttgtttctag gaaatgccaa aggaggcagt catgtttgag
     4921 gttttcaaag agtattcttt ctggacagtt gctttaggga ccgtcagcct agctgtagca
     4981 gccagcacta tcggaagtat atctgttctt actaaacaga gtttgcttgg ggatgctctt
     5041 gggcatgctt cttatcctgg agtgattgtc tctttcatga tttttcagtc gcgacatcct
     5101 ctctacttgc tgttaggagc agtgttgtca ggttaccttt cttatgcact tgtccattgg
     5161 ctgcgtcgaa aaggaggtca cagcctagtc aatgcccttt cattggtgtc tgcttctttc
     5221 tttggtttgg gaatggtctt gaaaaatgct attcagggaa atgaagcctt tgcaggggct
     5281 tcccaggctg gattgcaaac ctatctattt ggacaggcag cctttattca gctggatgat
     5341 gtgatactca tcggaattat ttctctattg gctttggcat tgtttgcttt cttttatcaa
     5401 gactataagc tatatctgtt tgatcaaact tttgcaaggg ttattggtgt tcgtgtaaag
     5461 tacttgcagc agttgacgat gtttttgatg atttgcctaa ttgcagtagg attaaagtta
     5521 gtcggagcta ttttgatgag tagttttttg attgcaccag ctgtctttgg cttgatgctg
     5581 ggaaaatcgt atcacaagag tctattactg gcaggaatcg ttgcaattgg gtcggctttc
     5641 gtagggacct ggattagctc cagtgtatct ggtttgtcga ccggaccgac tattatcgtc
     5701 tgtctgacag gtctgaccct gtctgcattt gtgtatatta cctatgtcag aaaggagaat
     5761 ggtcgtgttt gaagtattac tcattttgat ggttattgct agttcttgtg gcttgctcgg
     5821 ctcgatactg gttgtaaaaa atcaatcaat gctggcagat gcgctatcgc attctgtttt
     5881 gcttgggatt gttctgggat tttttattag tcatagtttg gattcgccat tgctaatagt
     5941 tggtgccagt ctgtttgggt tattatcggt tcttgccatt gaccgtctgc atagtcgaaa
     6001 gatagcgcat gatgccgcaa caggtctagt tttttctttc ttttttgcgg tggcggtttt
     6061 acttatttct ctttttgctc gcaatgttca cttggatgtg gatctggttt tgcaggggga
     6121 agtacttttt gctccgctcc atcgtatgga tgttctagcc tggtcactcc ctgttagtct
     6181 ggtcaagtcg agtttggctt ggttggttat tgttctattt tttgcgtggg cttaccatcg
     6241 tttacaagtg tacctatttg acagcaacca tgctcgtttg tctggcttac gaacaaggat
     6301 tttagaaatg gttattttga ttctagtttc cttaactact gttctggcct ttgaagcgat
     6361 tggttcgatg actgttattg ttttcttggt ggcaccaagt atggctgccc tatgttgggt
     6421 caaatccttt tggcaattgc ttcttttagg gcaaggtatc gctattctaa cggttgtgct
     6481 aggtttttta gtggccaatc aactggattt aaccatgtca gggacctgtg ccgttgtcag
     6541 ccttcttgtg gtttgttcga gtattattct aaaaaatact tggtcacggt catctgataa
     6601 gtgaaattgt cagaattttt tgttattata gtcattaaga aagacaaagg agaaaatcaa
     6661 tgatacgttt acaagatgat ttttacgaat acgtcaatgg tgaatgggca aaaacagccg
     6721 tgattccaga tgataagcca agaacaggtg gtttcacgga cttggcggat gagattgaaa
     6781 agctcatgat tgacacgaca aacgcttggt tggcgggaga agatgtccca gaagatagcg
     6841 ttttgcaaaa ctttgtggct tttcacaaac aagtagctga ctatgaaatc cgtgaccgtt
     6901 tgggtgcaga acctgcccag gccttgattg cagagtacaa ggctctcaac tctttcgaag
     6961 aatttaccag caagctggca gaatatgagt tggcaggcaa gccaaacctc atgcctttcg
     7021 gtgtggcacc tgactttatg gacgcgacca ccaatgtcct ctgggcagat tctctaggca
     7081 ttatcctgcc agatacgacc tactatgaag aagggcatga aaaaggagca gagttgctca
     7141 aaacgtggag agagagccaa gaagccctct tacctaagtt tggtttcagt gatgaggaaa
     7201 tcaaggacct cttggacaag cgtttggaac tagatgccac gattgccaag tatgttctgt
     7261 caaatgaaga gggttctgag tatgcaaaac tctatcaccc ctacgagtgg gcagatttta
     7321 ctgccttggc accagagttg cctttggatg atttcttcac agcgatttta ggtcaaacgc
     7381 cagacaagat tatcgtgcct gaggaacgtt tctggcaggc tgcaaaggac atttactgtg
     7441 cggataattg ggagcttttg aaggcgacct tgatcttgaa agcggcagga gcttatacag
     7501 ccttcctgtc ggatgaaatc cgtattctag caggagccta cagccgtgct ctttctggta
     7561 caccgcaggc gcaaaaccaa gaaaaagcag cctacaactt ggcacaaggc tactttaacc
     7621 aagccctcgg tctttggtat gcaggagaaa aattctcacc agaagccaag gccgatgtgg
     7681 aagccaaagt tgccaagatg attgaggttt acaaatctcg tttggaaacg gcagactggt
     7741 tggctcagga aacgcgtgac aaggccattg tcaaactcaa tgttatcaag ccttatatcg
     7801 gttacccaga agccttgcca gagcgttatt ataagaagct tgttgaccca agcaagtcct
     7861 tggtggaaaa tgccatcgaa ctaaacaaga ttgacattgc tcatggttgg agcaagtgga
     7921 acaagcctgt ggacattaag gaatggggca tgccagctca catggtcaac gcctactaca
     7981 atccacagaa gaacttgatt gtcttcccag cggccatttt gcaggcgcct ttctactctc
     8041 tggagcagtc ttcatcagct aactacggcg gtatcggtgc ggttattgcc cacgagattt
     8101 ctcatgcctt tgactcaaac ggtgcttcct ttgatgagca tggtagcctt aataactggt
     8161 ggacagagga agactatgct gcctttgaag ctcgtactca gcaggtcatc gaccagtttg
     8221 agggtcagga ttcttacggt gccaaaatca atggtaaatt gaccgtgtca gaaaacattg
     8281 ccgaccttgg tgggattgcg gcagccctag aagcagccaa gtcagaagat gacttctccg
     8341 cagaggaatt cttcactaac tttgctcgca tctggcgaat gaaggctcgt cctgagttca
     8401 tgcagatgtt ggctagtgtc gatgtccatg caccaggtca cctacgcacc aacatccagt
     8461 tgccaaactt cgatgaattc catgaaacat ttggcgttca agaaggcgac ggcatgtggc
     8521 gtgccaaaga agaccgtgtg attatttggt aaaaatggga aaacagctag tgaaaactgg
     8581 ctgtttttgt ctttcccaat tcccccaaaa cgtgatataa tagtaaagat aatttattga
     8641 catggagaaa tgacatgagc ttctataatc acaaggaaat cgagcccaaa tggcaagaat
     8701 tttgggcaaa aaatcatact tttaagacag gaacagatgc agaaaagcca aacttttatg
     8761 ccctagatat gttcccttat ccgtctggtg cgggcttgca cgttggtcac cctgagggct
     8821 atacggcgac ggacattctc agccgttaca agcgtgccca aggttacaac gttcttcacc
     8881 caatgggttg ggatgccttt ggtctgccag ctgagcaata cgctatggat acaggcaatg
     8941 acccagctga ctttacggcg gaaaatattg ccaacttcaa acgtcagatt aacgcccttg
     9001 gcttttctta cgactgggac cgcgaggtta atacgactga ccctaactac tacaagtgga
     9061 ctcagtggat tttcaccaaa ttgtatgaaa aaggcctagc ctatgaggca gaagtgcctg
     9121 taaactgggt agaagaattg ggaacagcta tcgccaacga agaggtcctt cctgacggaa
     9181 cgtctgaacg tggtggttat ccagttgtcc gcaagccaat gcgccaatgg atgttgaaaa
     9241 taacggccta tgcagagcgt ttgcttaatg acttagaaga agttgattgg ccagagtcta
     9301 tcaaggatat gcaacgcaac tggattggca agtctacggg tgccaatgtg actttcaaga
     9361 ttaaggacac agataaggac ttcacagtct ttactacccg tcctgacacc cttttcggtg
     9421 cgacctacgc agtccttgcc cctgagcatg acttggtaga tagtatcaca tcagctgaac
     9481 aagcagaagc tgtggcagaa tacaaacgcc aagcctctct caaatcagac ctggctcgta
     9541 ctgacctggc taaagacaag accggtgtct ggacaggtgc ttatgccatc aaccctgtca
     9601 acggtaaaga aattccaatc tggattgccg actatgtact ggcaagctac ggaacaggtg
     9661 ctatcatggc cgttcctgct catgacgagc gtgactggga atttgccaaa cagtttggtt
     9721 tggacattat tccagttctg gaaggtggca atgtagaaga agctccttac acagaggacg
     9781 gtgcccacat caattctgat ttcctagatg gtcttaacaa ggaagaagct attgccaaaa
     9841 tggtagcttg gttggaagaa aacggtgtcg gtcaggagaa gatttcttac cgcctccgcg
     9901 actggctctt cagccgccag cgttattggg gtgagccaat tccaatcatc cactgggaag
     9961 atggaacttc cacagccgta ccagaaaatg agttaccttt ggtattgcca aaaacctcag
    10021 acatcaagcc ttcaggtaca ggggagtcac ctcttgctaa cttgacagac tggttggaag
    10081 tggtgcgtga agacggtgtt aaaggtcgcc gtgaaaccaa cactatgcca caatgggcgg
    10141 gttctagctg gtactacctc cgctatatcg acccgcacaa tgatgagaaa ttggcagacg
    10201 aggatcttct caaagcttgg ttgccagtgg acatctacat cggtggtgct gagcacgcag
    10261 tccttcacct gctctacgct cgcttctggc acaagttcct ttacgacctt ggcgttgtgc
    10321 cgaccaaaga gccattccaa aaactcttta accaagggat gattttggga actagctacc
    10381 gcgacagccg tggtgccctt gtagcgacag ataaagttga aaaacgcgat gggtccttct
    10441 tccacatgga aactggtgaa gagttggagc aggcacctgc taagatgtct aagtctctta
    10501 aaaacgtggt taacccagat gatgtggtcg agcaattcgg tgcggacact ctccgtgttt
    10561 atgaaatgtt catgggtcca cttgatgcgt caatcgcttg gtcagaagaa ggtctcgaag
    10621 gcagccgtaa gttccttgac cgtgtctatc gcctcttaac cactaaggag cttgtggcgg
    10681 aaaatagcgg agcgcttgat aaagtttaca atgaaactgt gaagacggtg acagagcata
    10741 ttgaagacct gaaattcaac acagctatcg cccagctcat gatttttgtc aacgcagcca
    10801 acaaggaaga caagctttat gttgactacg ccaaaggttt tgtccaattg atcgccccat
    10861 ttgcaccaca cttggcagaa gaactctggc agggtcttgc aaatacaggc cagtcaatca
    10921 gctatgtcgc atggccaaca tatgacgaaa gcaagctggt agaaagcgaa gtggaaatcg
    10981 ttgtccaaat caaaggcaag gtaaaagctc gcttgaccgt agccaaagac ttagcaccag
    11041 ctgaacttga aaaagttgcc cttgcagatg aaaaagtcca agctgaaata gctggtcaaa
    11101 cagtggtgaa ggtgattagt gttccgaata aactagttaa tattgttgtg aaataaatgt
    11161 aaataataag aaaacgactt ccgtattgga ggtcgttttt gtattttcta gaatgatcat
    11221 gttgtcttaa tttccgttgt gatacggaaa aagagggtct gtgtatccgt ttacaataga
    11281 tgttcactga aatataataa gaatataaaa gaagatgagg gagggaaagc gtgttgaaaa
    11341 ctcttaaatt agtgacaatt ggtggaggct caagttacac tccagaattg gttgaaggta
    11401 tgattcttcg tgcacatcaa ttacccattc gagaatggtg gtttgttgat gttgaacaag
    11461 gcgcaaaaaa acaggagatc gtagttgagt tagcacggcg aatggtcgca aaagcaggcc
    11521 tggactggaa gatagagtcg accttagaca gaagagaagc attgatagag gctgattttg
    11581 taacgtctca gttccgagtt ggccaactac ctgcaagata cttagatgag accattccct
    11641 tgagttatgg gatgctgggg caagagacca atggggctgg aggaatcttt aaagcattta
    11701 ggaccattga agcatataaa gggattatca aggatatgaa ggacttgtgt ccgaatgctt
    11761 ggttgattaa ttttacgaat cctgctggta tcgtaacgga agccattgct aatgaactcc
    11821 actggaagaa agttattggt ttgtgtaata tccctattgg tcaaaagaaa cttgctgctc
    11881 agattttagg ccaatcagaa ggagacctaa gtacgcgcca tattgggtta aatcattttc
    11941 actttcatga agtttgggat aagtatgggg aaaatcgtac gcaagtaatc ttagaaacat
    12001 tgtatgggga tttgagagag gagatgccag aagcagtcaa aaatattact aatctagagt
    12061 ttcctattca attgcttcgg acgattggaa tgttgccatg tgattaccat cgttattatt
    12121 ttttggaaca agaaatgtta gaggatgctt tggaacagta tcgtcagggt aatgtacgag
    12181 ccaaggttgt tcaagaagta gagacagaat tgtttgagct ttacaaagac ccacaattga
    12241 aagacaaacc aaaacaactg gaactacgtg ggggtgccta ctatagtgat gttgcctgtc
    12301 aaattatttg tgctatccat aatgatagta gagaagaatt gaccgtatct actgtaaata
    12361 gaggagttat tccctatcta cctgaaaatt gtgtggtaga agtcacctcg attatctctg
    12421 cgcaaggagc aactccacta gcttgtccac caactccgcc gattgtaacc ggctatttac
    12481 aactgatgaa gcaaatggag cttgtcactg ttcaggcagc aatgacaggt gattattccc
    12541 tggctttaca agcttttaca ctcaacccgc ttatttcaaa tggaccacaa gctgaagtgc
    12601 tattacaaga tatgttactt gcacatgaga actatttgcc acaattcgca ccagctatac
    12661 agcacataaa agagagaagg aacaatcaat gactaaagac aaggtatttt atcaatttcc
    12721 aacaaatttt ttgtggggtt cttctacatc aggacctcaa agtgaaggaa tcgagccagg
    12781 tgatggaaaa ggacagaaca actgggatta ttggtatagt attgagcctg aacgatttca
    12841 taacaaaatt ggccctagtc aaacatcaac attttacaaa cattatgaaa aagatattga
    12901 tttactagtt gaaactggac acactgtatt tcggacttcc attcaatggt cgcgtctcat
    12961 accaacaggt actggagaaa ttaatcaaga ggcggtgtcc ttctatcgtt cggttttttc
    13021 tttggtaaga gcaaagggaa ttcagctctt tgtcaatctt tatcactttg atcttcccta
    13081 ttgtttacaa gaaaaaggtg gttgggaaaa taaggaaatt gttcgagcct atgaagcata
    13141 tgcacgtaca tgttttgaat tgtttgatga tttagtcgat cgttggatta cctttaatga
    13201 gccgattgtt ccagtagagt gtggttatct aggaaattat cattatccat gtaaggttga
    13261 tgcaaaagcg gcagttgcgg tggcttatca tacacagttg gcaagttctt tagctgtaaa
    13321 ggcatgccat gaggtaaatc ctcacaaaaa aatcgctatt gtcttgaatc taactcctgc
    13381 ctaccctcgt agtgagcaag cagaggatat aaaagcagct cgaattgcag aattgtttca
    13441 aacaaagagc tttctagatc cgtctgtgtt aggagaatat cccgatgact tagtcgagct
    13501 gattgatgca tatggtctaa tgccagaggt cagcgatgag gaattagcca ttattaagga
    13561 aaatcgtgtg gacttcctag gggtgaatta ttatcaaccc cttcgggtac aagctccaac
    13621 caatagttgg aaagagggag agcctctaac tccggaatat ttctttgccc catatgatat
    13681 gccaggtaaa aaaatgaatc cgcatagagg ttgggagatt tatgaaaaag ggatatacga
    13741 tattgcgata aatattaagg aaaattacca taatatcgaa tggtttgtaa cagaaaatgg
    13801 tatgggagtt gaaggagagc gcgcatttaa agacaatgga ctcattcaag atgattaccg
    13861 gatcgaattt atcgaagatc acttaattga attacatcga gctatccaag aaggggcgaa
    13921 ttgtaaagga tacatgttat ggacctttat tgattgctgg tcttggttaa atgcatacaa
    13981 gaatcgctat ggattagttg aactggatct agacacgcaa gaaagaattg ttaagaaatc
    14041 aggcaagtgg ttcaagaaat tgagcgatgc taatggtttt catcgctagg agggaatgat
    14101 ggtagaactg ggcttttcaa tttatcctga aaactattcg ctagaagaga gtcaagctta
    14161 cattaaactt ctgcaccaat acggagcaag gcgcttattt atgagtttgt tgcaacttga
    14221 aaatggagac cgaacagttt tcgaattgta tcaaaaacta attgactatg caaatcaact
    14281 ggggatgact gtcattgcag atatcagtcc ccattttatc gcagcgcaag gttgggatgg
    14341 ggcgttgatg gagcgtgcag cagaactggg gttggccggg attcgtcttg atgaagctct
    14401 tcctttggaa gaaatcattg agatgacaca taatccacgc aacataaaag ttgaattgaa
    14461 tttgagtaca gataagatct tgctgtccaa tcttttggct agtgttgcaa acagggataa
    14521 tattattgcc tgccataatt tttatcctca tgcttttaca ggtttatccg aagagcactt
    14581 cttagagatg tcatcttttt atcatcaatc aggtataaga actgcggcat ttgtcagtgc
    14641 acaaacagct tctgaaggac cgtggccatt atctgaaggc ctaccaactt tagaagatca
    14701 tcggttctgg cctcttccct tgcaaattgc ttggatgaag gcaacgggtt ttattgattc
    14761 tattttgatt tcaaaccaat ttatctcgga ggaggaacta caatccattc aaacagtttt
    14821 aaatgaagat acaatcagcc tcatggttga tattgaggag ggtttgactc ccgttgaaag
    14881 agagattgta gcgtttaatc atgtttatag aggtgatatt tctgcttatg ttctgcgttc
    14941 aaccatgccc cgtgtggtgt ataaggacgc atctgtaccg gctcggtcag accaagcaat
    15001 tcctgtggga cgaggtgata tcttaattga taatgattta tatggtcgct acaagggtga
    15061 attgcaaatt gcactcaaag aatttaccat cagtccaaag gtaaataagg tcggacgaat
    15121 cagcccagac tatcttccct tgttacgctt tattaaaccg tggcaagagt ttaaactggt
    15181 catagcttcc gagcactttc attaatgatg cggaaaaagt aggcacctcc agaaatcgct
    15241 ttcatgttat aatgtacata taagaaatca aagactgttg gaggaaagga gggagcatgt
    15301 tatctaaaaa agaagtacgc ttgttagagg ctttacttgc aaaacaggag acattcgtac
    15361 caagcatcga actagccaag gtattatcag tgagtgatcg aacggtaaga acgtatattc
    15421 atcaactgac atcttcttta gaaaatagcg gtgctcatat cctttcaaaa caaggtagtg
    15481 gttattgttt acagattgaa cggccagttg agtttgaaat gttttggcag gagcaattgc
    15541 tcctaaaaaa gcggttaact gatttaactc aactagaaga agcgggggat cggcaacggt
    15601 atattctcaa taagctcttg tttgaggatc cgattcagtc accagattgt ttacaaagag
    15661 agctattcat tggaaagact acgcttcatt ccattttggc ggatattcga aaactattta
    15721 tcccctatca attagaactc cttgtgacac gtaaaggaat tgaactaaag ggggatgaac
    15781 ctgctatccg tcactttatt atggattact tttttgatga gggaaatgtt caatctgttt
    15841 atggggttgt cgaaaccaat ctattgccag atatacggtt cacagactta atgttaatcg
    15901 tcttagacga gtgtcgggat gctaaattac gtttatcgga ttttgtcatg cagaatctag
    15961 tgctccatat cgctcttctt ttacaacggt tgaaaatcgg gtctggtcta gaccgattcc
    16021 cgataccgga gcgtattcag tcgtctcaag agtatcttgt ggcacaacgg attatagacc
    16081 gaatcgaaga aacgtttcaa gtgacgatac cgtcagagga agcaaattac attgcgttgc
    16141 accttaaggt aaaactaacg gggaatccaa atcaagatgg tgaggtggaa gagatattta
    16201 gacagaatgt ggagaatagt cttttacatt tgtcacattt aacaggccta gagttgtcaa
    16261 aggatcagca gctattcaaa ggagttctgg cccatttgat gccgttacgt attcgtttgg
    16321 agaataatat tcaactggga aatcctctaa ctgctgaaat taaagaccaa tattttgagg
    16381 ctttcgaatt aacaaaaatg gcatttgcag cgataccaga actaatgggg tattcgattt
    16441 cggatgatga gtgggcttac cttaccttac atgtgatggc ggcaattgaa cgctatcagg
    16501 gatttaagaa gaccagagta ttggtagtct gtgctaccgg aattggaagt gcattgatgt
    16561 taaagaatcg cttggagaaa gaattttcat ccagcttaga aattcttgat acggttagtt
    16621 attatgaagt tacagaggaa cgactgaagg atgttgattt gattatttca tccattagct
    16681 tgtcgaatct aatttttatg acaccggtta tcaatgtcag cgtcttccta tcagagaagg
    16741 acattgaggc tattcgatct catttacagc aaacgacccc agttattcct gaccaagggg
    16801 aaaatgtgct aatggaagta ggtcaggcta agcagattgc agaagcagta tttgctccac
    16861 atagaataat ctatcttgag gaggattgtg atcgagaagt agccttgaag caactagtaa
    16921 cctctttgga cgaatccatt gagcctgatt ttgttaatcg ctttatagaa caagtgaatt
    16981 tacgagaatc ttacagtccg gttgtctttg ggaatcggtt agcctttcct catccagcct
    17041 tgccgatgag ttattcagaa caaattgttg tagggatata taagaatgct gtggattggg
    17101 gccatggaaa gcaagttcaa ttcgtcttcc tattgtcacc gtcaaagggt cgaaatgcat
    17161 ttctgaagta tatctcacct tgtctggttg attttgttca agatagagtt ctccaagaac
    17221 gtttgttaca tcatccaaat tataaagaat taattgatat ttttattccc ctcattcaaa
    17281 gaaaggggta ggaggagttt tttatgaaac acattatgtt agtttgtaac gcaggcatgt
    17341 ctacaagtat gcttgtaact aagatgcaaa aagcagcaga agaaacaggg gttgaagcaa
    17401 ctatttgggc tgttccagta tctgaagccg ataatgaagt tgctcaaaaa gagattgatg
    17461 tattactctt aggtccacaa gtaaaattcc tattgaacga ctataaggca aaatttgagc
    17521 caactattaa ggtagatgct atcaatatga tggattacgg agtgatgaac ggagctaagg
    17581 tgctagaggc tgccctaaaa atgatggagg ggtagtatga attctccaga gtatcttgaa
    17641 gcgattatgg ggttaatcat gtacggtggt gaagcaaagg gcttcgctag ccaggcgatt
    17701 caagcagcca aagaaggtcg tattgacgaa gcggaacagc tgctccaaga cgcaatagct
    17761 tcattgaata ttgcccataa atcgcaaaca caattattag caaaagaagc aagtggagag
    17821 tctgttgaat tatctttgtt aatggtacat ggacaagatc acttgatgaa tgctttgaca
    17881 tttattgatt tagcgaaaga gttcgtagat ctttataaac agattggata attataggag
    17941 aactgggtta tgaagatatc aaaaataggt caagttccaa ttgagcgact tttcgctact
    18001 attcgccaat ccttaaaaga tgattttcag caaaatacgg gttccatctt gcaagatcaa
    18061 gacattatcc agggacttac ttatactaaa acatttggtg ataagggaca acatagtgtc
    18121 aaggtaactg tagaagagtt tacagctcct tattgctatg cagtttgttt ccattctaat
    18181 cgaggaaaag agcatgttcg ctatcagtta gaggatttag ggaatgatca aaccaatata
    18241 gaatattctt atgagatgga ggctggtgat atttttgtaa aaggcaatca atttctcatg
    18301 gaaaaaatct tttcaaaaag tataaaacgt cagacaaatg cccagttaga ggcgctgatt
    18361 cgttgctcag ttgagccggt atcagtttaa catttaggaa atataaaaca aaaaatagaa
    18421 aaggagacag aatatgtcaa atatttctga gaagttcatg gaaatatctg gaaaaattgg
    18481 ttcgcaacgg catttgattg cgattcgtga ttcctttatt tctatgatgc ccattacgat
    18541 ggcaggtaca gtggccgttc tattgaatgt ctttctgaga gacatcccaa ataatatggg
    18601 atggactggt tttgctgatg ccatgcaacc agtgatagat atcaatggtt acgtttattt
    18661 tggaactatc gggataatgg cacttgtatt tgcctttacc tttggttaca atttgtcact
    18721 catgtataag gtgaatcctt tagcaggtgg tttaatttcc tttgcatcat tcatctctac
    18781 tattcctcaa agtgtaacga tttccactgc tttagacgga gttgataagt cagttgtttc
    18841 tgctttggaa ggtttgggat tatccattgt gacgacagat ggagtttcag ccttggaaac
    18901 aagccagtgg ggagctatcg cattgaaata tggcggtgca acaggtttgt ttacagcatt
    18961 gtttattgga tttctcgcta catgggtcta tgcaagtctt gtaaaacgga atgtcattat
    19021 taaattgcca gattctgttc caccggcagt taataaggcc tttgctgcta ttatcccagg
    19081 tactgctgca atttatgcgt catccattgt ggcatatctt gcatttactc taacaggaca
    19141 atctttggga gatttagtat caacttatat tcaactgcca ttacttggtc tgtcacaagg
    19201 tcttgggtca gtcattcttt taactttcct tgtacaactt ttttggttct tcggcctgca
    19261 tggtcacaat gtcttggcgc caattatgga cggagtctat atggttgcac taaatgaaaa
    19321 tgcggctgct tatactgcaa ctcatagtac tgcgaattta ccgtggcttt ggactcgtgg
    19381 ttcctttgat gcttatgctc aaatgggtgg ttcaggcgtc actctagctt tgattattgc
    19441 tattttcatt ttctcaaaac gagaagaata caaaatggta gctaaattat ctgctccaat
    19501 gggtgtattc aatattaacg aaccagttac ctttggtata ccaatggtat tgaatccact
    19561 gtttgctatt ccatggctaa ttgttccacc gatttgtgca actatcgcat acttagcgac
    19621 agcggcaggt ataattccgc cagtctttgc tcctgttcca tggattaccc ctccaggact
    19681 ctacgcttat ctagcaacag gtggtaacat tatggctgcg ttggtttcac tattcaacct
    19741 ctttgttgcc ttcctgattt ggacaccatt tgttattgca gccaataagg ttaaggctgg
    19801 tgaggaataa tgttttccaa gaacaaatct cctttcttat tggcgctagg atttttcctt
    19861 atcgcgatta gtccgcttta tgggaataaa tatctaaaca ttatatctgg tttggttttt
    19921 attctaatca gtgtactcag gagtaggaga aaataattac actaacacta aatagaaaac
    19981 gacttccgta ttggaggtcg tttttttgac aaaaagtccg gacactgtga ttgttaagag
    20041 attgcaagtt atctgtaata tttgttacat tcctttaagc tctctgcaag gattatcctt
    20101 taggatagat agggcagaaa aacacgaagg aatcaaatat tatgagttat caaatgataa
    20161 aaaaacaatt caaattggga ctgtttacag ccctcatagg aaatctttta tggttgggaa
    20221 tggtgaatgt cagtgcagat gacacagtta taagcgatag tccgacagta tcagcgttaa
    20281 caaaaaaggt tgaagctgca aaaatagcag ccaaagctgc tgcagaaaaa attgtggctg
    20341 agaaagctgc tgccgctgca aaggctgctt tggctaccaa tatggtgtca gaggttgctt
    20401 tggaatatac ggctaatacc tatccagcgg gtcagtgtac ttggggtgcc aaagaaatgg
    20461 ctccttgggt tggaaactat tggggtaatg gtggagattg ggcagctagt gcggcagcgc
    20521 ttggatatga agtgggaact actccaaaag ttggcgctat tgctgtatgg acagatggag
    20581 gctacggtca tgttgcctat gtaacagatg tggcagctaa tggtcacatt caagttaagg
    20641 aatcaaacta tggtggagta tactacccaa gtaatgtccg aggtttcttt gatccaacaa
    20701 cgactagcga aggaacagtc agctatattt acccaccagc tggagtaaat taatatgaga
    20761 tataaagacc gagtaggtct ttttttatgc tccgatactt gattaaaatt tttaatgtcc
    20821 ccgatagaaa aaatatattg gctggaaatt gttattcatg atactattgt aaaatctgaa
    20881 aaagaaagtg tgttttaatt atgaagaaaa tagctatgtt tacaagttta ttgtttgcgg
    20941 gtatcgtgtt agcagcctgc aattcatctg caggtacatc gaatagtaca gaccaaaccc
    21001 tcttggcaca aattaaggaa gaaggcgtta ttcaaatcgg tactgaaggt gcctatgctc
    21061 cttactctta ccacgatgag agcgggaaat tggtcggtta tgatgtagaa gtagccgaag
    21121 ccgttgctga gaaattgggt gttaaggtgg aatttgttga aacagaatgg gattccatga
    21181 ttgcaggttt ggatgcggcc cgttttgata ccatcgctaa ccaagtcggt attacagatg
    21241 agcgtaaggc aaaatacgat ttttcaacac catatacata catctatgga gccttggtga
    21301 cgcaaaaaga taatactgac attacaagct ttgcagattt atctggtaaa aaatctgcca
    21361 acagcttgac aagtaactgg gcagatttgg ctcgtgaaaa tggtgcagaa gttgtcggtg
    21421 tggatggatt ctctcaggca gttgaattgt taaatacagg acgtgtggat gctaccatca
    21481 atgacaactt ggtgtacttg gattatgtga aacaacatgc tgatgcaccg attaaggttg
    21541 tgaccttgac ggatgacgta tcgacaacag ccttcccagt tgcaaaagga aatgaagatc
    21601 ttgtgaaaga gattgatgca gccttggcag aattggctag tgaaggaaaa ttagctgaaa
    21661 tttcaaacaa atactttggt gaagacgtat caaaagctaa gtaatcagga gaaacaatga
    21721 cagagagagt atggcaacta ttgcttgatt cattttcaca aatacttgtt ccaggattgt
    21781 tggtgactat cccactgaca atcctgtctt ttacctttgg cttgttaatt gctattggga
    21841 cggccttggt gcaaattgct cagattccca ttctcaaaca agtggctcgt ttctatattt
    21901 gggttgttcg agggacacca ctcttggttc aactctatgt gatattcttt gggctaccaa
    21961 gtctaggaat tgtcttggat gcttttccgt cggcggtttt ggttttttct gtcaatacag
    22021 gtgcctacgc tgcggaaacc atacgggctt ctatcgagtc cgtgcctaag gggcagttgg
    22081 aggctggcta ttctgtcggg atgagttttg ctcagaccat gcgtcggatt attctcccac
    22141 aggcctttcg agtggccttc ccgcccttgt ccaatacttt gattggattg gtcaaggata
    22201 catcgctggc tgccaatatt accgttttgg agatgtttat ggcaactcag cagattgcag
    22261 caaggaccta cgaaccgttt gccctctatt gtgaggtggc ccttgtttat ctattctttt
    22321 caactatttt gaccaagctt caagcctatg gtgagaagaa gttggcagtc tattaggagg
    22381 ttcagatgat tcaagcaaat ggagtaaagc tgaccttcgg tggtcagcag gtgttaaaag
    22441 ggattgatgt agaggtcaat ccaggtgagg tggtcgtcat tttaggcccg tctggatcgg
    22501 gaaaaacgac tcttcttcgt tctttaaact ttttggaaaa agctgacgcg ggtcacttag
    22561 tcttcggtga ggacagctat gacctggctc agatgaccaa gaaggaaatt gcaggcattc
    22621 gcaagcgcac agccttcgtt tttcaaaact ataatctctt tgccaacaag actgccttac
    22681 aaaatgtgac ggaaggcctg attgtggctc ggaaagtacc caaacaagag gctgaaagaa
    22741 tcggtaaagc agccttggac aaggtgggac tatctgataa atatggtttc tatccaagtc
    22801 agctgtccgg tggtcagcag caacgggtcg gcattgccag agccattgcg accaatcccg
    22861 aggtcatcta ttttgatgaa ccaacctcgg ccttggatcc tgagttaatc ggtgaagtcc
    22921 tggctgtcat gatgcagttg gcaaaagaag gtatgaccat ggtggttgtg actcatgaaa
    22981 tgggctttgc gcgtgcggta gctgatcgag tcatatttat ggatggtggt cggattatcg
    23041 aagaaggtcg accagaagat atttttgacc agccaaaaga agaacgtact aagcaattct
    23101 tagctcgcat tcaaaaggta gatgttgagt aagctaagca aattaattta tcttattcat
    23161 ttccataatc gtacagaaaa tggacagaat ttgacaaaga tacttcaatt gttagacatt
    23221 gattggtcta gcagttggag tattttttgt ttagagggaa tgggtattct tatatagtac
    23281 ttttagcacg aggcgatgag ccgcaggcat agatgaagtt aggcaaggtg agctaaggaa
    23341 gtaatagaa
//
LOCUS       DBNFCK010000019        23027 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.019, whole genome shotgun sequence.
ACCESSION   DBNFCK010000019 DBNFCK010000000
VERSION     DBNFCK010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 23027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23027
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.019"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..583)
                     /gene="nth"
                     /locus_tag="AC1PIW_000587"
     CDS             complement(<1..583)
                     /gene="nth"
                     /locus_tag="AC1PIW_000587"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HIP6605548.1"
                     /translation="MVLSKKRARKVIEEIIALYPDAKPSLDFRNHFELVCAVLLSAQT
                     TDAAVNKATPGLFAAFPTPQAMAAAEVKDIEPYISRLGLYRNKAKFLKDCAQQLMERH
                     NGIVPQTREELEALAGVGRKTANVVLSVGFGIPAFAVDTHVGRICKHHDIVKKSATPL
                     ETEKRVMEVLPPELWLPAHQAMIYFGREVCHPKN"
     gene            681..1532
                     /locus_tag="AC1PIW_000588"
     CDS             681..1532
                     /locus_tag="AC1PIW_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HIP6605549.1"
                     /translation="MDLQELCRKVIQARPRNSHKGSYGRVLLIGGLYPYGGAIIMAAL
                     ASVNSGAGLVTVATEKDNIPALHSHLPEAMAFSFDDQALLISSLENADLVLIGPGFGE
                     NSRAEQLLDFVFEHLSDQQILVLDGSALTLVAKQNRKNFPCKQVILTPHQKEWERLSA
                     IPVADQTSQRNLEALQSFQENTILVAKSSATQILKTQPNQVTPIGAGGPYQATGGMGD
                     TLAGMIAGFAVQFPLVQLYERILVATYLHSYIADQLAQDYYLVKPTDVSRNIQKTMLQ
                     FQTQTTA"
     gene            complement(1714..6471)
                     /locus_tag="AC1PIW_000589"
     CDS             complement(1714..6471)
                     /locus_tag="AC1PIW_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIP6605550.1"
                     /translation="MKKKETFSLRKYKIGTVSVLLGAVFLFAGAPSVAADELTSLVET
                     KVEATVPDVIVSESASESPVVEELVDTSVEATPTDVTTTDNVEETLGSEALENITNTE
                     VEATQPAVETPAISEKKVEEEEKLAVADETTAITNQEEAKPQNIDSNTIITVPKVWDS
                     GYKGEGTVVAIIDSGLDVDHDVLHISDLSTAKYKSEKEIEAAKEAAGITYGEWFNDKV
                     VFGYNYVDVNTVLKEEDKRSHGMHVTSIATGNPTQPVAGQLMYGVAPEAQVMFMRVFS
                     DLKATTGAALYVKAIEDAVKLGADSINLSLGGANGSVVNMNENVTAAIEAARRAGVSV
                     VIAAGNDGTFGSGHSNPSADYPDYGLVGAPSTARDAISVASYNNTTVGSKVINIIGLE
                     NNADLNYGKSSFDNPEKSPVSFEIGKEYEYVYAGIGQASDFDGLNLIGKLALIKRGTI
                     SFSEKIANATAAGAVGVVIFNSRPGEANVSMQLDDTAIAIPSIFIPLEFGEALASNSY
                     KIAFNNETDIRPNPEAGLLSDFSSWGLSADGELKPDLAAPGGAIYAAINDNDYANMQG
                     TSMASPHVAGAAVLVKQYLQATYPTKSPQEIEALVKHLLMSTAKAHVNKETTAYTSPR
                     QQGAGIIDTAAAISTGLYLTGEDGYGSITLGNVEDIFSFTVTLHNITNEDKTLNYSTQ
                     LTTDTVQNGLITLAPCLLAEIPGGKVTVKANSSTTVTINVDAASFAEELTGLMKNGYY
                     LEGFVRFTDVADVGDIVSIPYVGFRGEFQNLAVLEEPIYNLIADGKGGFYFEPVTAQP
                     DTVDISHHYTGLVTGSTELIYSTDKRSDFAIKTLGTFKNEAGYFVLELDESGKPHLAI
                     SPNGDDNQDSLALKGVFLRNYTDLVASVYAADDTERTNPLWESQPQSGNKNFYSGDPK
                     NPKSSIIYPTEWNGTDSEGNALADGKYQYVLTYSSEVPGAAVQTMIFDVIIDRESPVI
                     TTATYDETNFTFNPRPAIEKGESGLYREQVFYLVADASGVTTIPSLLENGDVTVSDNK
                     VFVAQNDDGSFTLPLDLADISKFYYTVEDYAGNISYEKVENLISIGNEKGLVTVNILD
                     KDTNSPVPILFSYSVTDETGKIVAELPRDAGDTSVLKLPFGTYTFDLFLYDTEWSSLA
                     GETKAVVTISEENSTAEVNFYVTLKDKANLLVDIDALLPSGSTIQLVTADGQTIQLPN
                     AKYSKTDYGKFVPVGTYTILPTLPEGYEFLEELDVAVLANQSNVKKLTLINKVALKEL
                     IAELAGLEETARYYNASPELQTAYAKALEDANAVYANKHNQVQVDSALANLVAAREQL
                     NGQATDKEKLIAEVSNYTPTQANFIYYNAENTKQIAYDTAVRSAQLVLNQENVTQAVV
                     NQALADLLAAKANLDGQKTDISALRSAVSVSSVLKATDAKYLNASENVKQAYDQAVEA
                     AKAILADESASQASVDQALAVLTSAQAELNGIATSTNDAKEPANTATDKKDEGTVTPP
                     PIDSEKVDVQAPPVKDTGNSGHVSIGQKPNPQPTLPRPVTLQASLSSPNQEKQVTQLP
                     NTGDNDTRYYLVLGVIIGLGTLLVSKRRHKEEV"
     gene            6925..7257
                     /locus_tag="AC1PIW_000590"
                     /pseudo
     CDS             6925..7257
                     /locus_tag="AC1PIW_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638881.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(7305..8843)
                     /locus_tag="AC1PIW_000591"
     CDS             complement(7305..8843)
                     /locus_tag="AC1PIW_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HIP6605551.1"
                     /translation="MENHRKEVSFISQSILYSVLRGSLVGIVAGLVVVAFRLAIEKLF
                     SVFTHLYSLATDNAIYLLLIGGLYLVIALLVGKLIKDEPNAKGSGIPQVEAELKGIMD
                     LNWWSVLWRKFIGGVLSIASGLMLGREGPSIQLGAVTAKGVSVLLKSSEMERRSLIAS
                     GAAAGLAAAFNAPIAGLLFVVEEVYHQFSRLVWVSALAASITANFISLHVFGLMPVLH
                     MPKDLQLLSLDQYWIYILLGVFLGLAGYVYEIVILNIQDFYTLLSKIIPLPVPYYSVF
                     AFLFIIPIGYFFPNLLGGGHHLILELPYLEQGLLTLAILILIRFVWSMISYGSGLPGG
                     IFLPILTLGSLLGLFVARFFLDIGFISQEQMLLFIVLGMAGFFAAISKAPLTAIILVT
                     EMVGSLNQLMVIGLVALSSYVMMDLLGGAPVYEAMLEKILPEEVEQQEHMTLIEVVVN
                     ETLDRRFVSELRLPDSCLITSQIHHGKSRIVKGNSQLSMGDSLLVAVPISQIHTVRRI
                     FEGD"
     gene            complement(8957..10027)
                     /locus_tag="AC1PIW_000592"
     CDS             complement(8957..10027)
                     /locus_tag="AC1PIW_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIP6605552.1"
                     /translation="MKRLYSFFVGIIAIVLVLWGISYKLEADSTQGTTDKLVIYNWGD
                     YIDPELLAEFTAETGIQVDYQTFDSNESMYTKIKQGGTTYDLTIPSEYMISKMTEENM
                     LIKLDKSKIEGIENIDPRFMGLSFDKNNDYSIPYFWGTLGIVYNETMVENPPQEWEDL
                     WSEEYRDNIMLIDGVREVMGFGLQSLGYSLNSNNPTEIEKAAEHLYNLTPNVKAIVAD
                     EIKGYMIQDAAAIAVSFSGEASEMLDGNENLRYVVPSKGSNLWFDNMVIPKTAKNIDG
                     AYAFMSFMLRPENALRNAEYVGYSTPIPAAKAMLDEETQNDESFYPSEETMKKMEVYD
                     NLGPEMLGMYNDLYLQFKMYRK"
     gene            complement(10027..10797)
                     /locus_tag="AC1PIW_000593"
     CDS             complement(10027..10797)
                     /locus_tag="AC1PIW_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6605553.1"
                     /translation="MKKFANIYLTVAFLLLYLPIFYLIFYAFNEGGDMNGFTGFTLEH
                     FSSMLGDSRLMLILAQTFLLAFLSSLIATIIGTFGAIYIYQAKPRFQNALLSVNNILM
                     VAPDVMIGASFLILFTMIGFQLGFVSVLLSHIASSIPIVVLMVLPRLKEMNADMISAA
                     YDLGATQPQMLKEIMLPYLTPAIIAGYFMAFTYSLDDFAVTFFVTGNGYSNLSVEIYS
                     RARQGISLEINALSTLVFLFSILLVVGYYFISREKEAN"
     gene            complement(10797..11597)
                     /locus_tag="AC1PIW_000594"
     CDS             complement(10797..11597)
                     /locus_tag="AC1PIW_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6605554.1"
                     /translation="MKKTSRLFAIPYALWVFLFVLAPVALIIFKSFFDIHGDFTLANY
                     QTYFNSPNMTYLRMSFNSIFYAGIITLVTLLVSYPTAYFLTKLKHRQLWLMLIILPTW
                     VNLLLKAYAFIGIFGQHGSINQFLEFIGLGAQQILFTDFSFIAVAAYIEIPFMILPIF
                     NALDDLDKNLINASRDLGATPWQTFTKVIFPLSMNGVRSGVQAVFIPSLSLFMLTRLI
                     GGNRVITLGTAIEQHFLTTQNWGMGSTIGVVLILAMLLIMWMTKERKK"
     gene            complement(11581..12735)
                     /locus_tag="AC1PIW_000595"
     CDS             complement(11581..12735)
                     /locus_tag="AC1PIW_000595"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6605555.1"
                     /translation="MKKPIIEFKNVSKVFEDSGTTVLKDISFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDASSGDIYLDGQRINDVPTNKRDVHTVFQSYALFPHMTVFENVAF
                     PLKLRKIDKAEIERRVTEALQMVRLSGYENRSIQKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTEMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNEGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGRMIEDYLVEFNGKRFEAVDGGMRPNEEVEV
                     VIRPEDLQITLPEEGKLQVKVDTQLFRGVHYEIIAYDDLGNEWMIHSTRKAIVGEVIG
                     LSFEPEDIHIMRLNETEEEFDARIEEYVEVEEVEDGLINAIEEERNEENL"
     gene            complement(12774..13682)
                     /gene="murB"
                     /locus_tag="AC1PIW_000596"
     CDS             complement(12774..13682)
                     /gene="murB"
                     /locus_tag="AC1PIW_000596"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIP6605556.1"
                     /translation="MKDKIRLELEGIDIRFDEPLKEYTYTKVGGAVDYLAFPRNRYEI
                     VRIVEFAKREGIPWQVLGNSSNIIVRDGGIRGFVIRMDKLNSVTVSGYTIEAEAGANL
                     IETTKVALFHSLSGFEFACGIPGSIGGAVYMNAGAYGGEVAHILVSAQILTPAGYVET
                     LDNRELRFGYRSSILQENGAIVLSAKFALRPGNHTVIQQEMARLTHLRELKQPLEYPS
                     CGSVFKRPLGHFAGQLIMDAGLKGYRIGGVEVSEKHAGFMVNIENGTASDYENLIAHV
                     IQVVEKSSGITLEREVRIIGDPADTL"
     gene            complement(13917..14777)
                     /gene="thrB"
                     /locus_tag="AC1PIW_000597"
     CDS             complement(13917..14777)
                     /gene="thrB"
                     /locus_tag="AC1PIW_000597"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HIP6605557.1"
                     /translation="MKIIIPATSANIGPGFDSVGVALSKYLTIEVFEETDEWVIEHNL
                     EHVPSDKNNLLIKTALKIEKGLQPHRIRMISDIPLARGLGSSSSVIVAGIELANQLAG
                     LNMTADEKLLKATEIEGHPDNVAPAIFGNLVISSYVNKRVQAVVTEFPEASFVAFIPN
                     YPLRTVESRGVLPSQMGYKKAVAASAIANVAVASLMAGDLEKAGKAIQSDMFHEPFRQ
                     LLVKEFCPIKQTAQELGAYATYLSGAGPTVMVLAPKDREDAIVLALEELNLDGTVHRL
                     QVDTKGIAIV"
     gene            complement(14781..16067)
                     /locus_tag="AC1PIW_000598"
     CDS             complement(14781..16067)
                     /locus_tag="AC1PIW_000598"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019782768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HIP6605558.1"
                     /translation="MVVKIGLLGFGTVASGVPFLLEENQEKIEKAAHDSIEVAKVLVK
                     DEAEKDRLLAAGHDYHFVTNIDDILADEDIAIVVELMGRIEPAKTFITHALEAGKHIV
                     TANKDLLAVHGSELRALAEEKGLALYYEAAVAGGIPILRTLVNSLAADKVTRILGVLN
                     GTSNFMLTKMIDEGWTYEKALQTAQELGYAESDPTNDVEGIDAAYKAVILSQFAFGMT
                     VDFEHVAHKGISNITPEDVAVAQELGYVIKLVGDIQETTSGIAAEVSPTFLPKNHPLA
                     SVNGVMNAVFVESIGIGQSMYYGPGAGQKPTATSVMADIIRIVRRLKDNTIGKAFNEY
                     SRPLQLAAPSDVKSEYYFSIDAPDKNGKMLRLAEIFNSEEISFKQILQQAADGETARL
                     VIVTHEMSKTQLENVTDKLSKETDFTVLNTFKVLGE"
     gene            16172..17095
                     /locus_tag="AC1PIW_000599"
     CDS             16172..17095
                     /locus_tag="AC1PIW_000599"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIP6605559.1"
                     /translation="MKHSRRARRSRTQKKQLVPMLLGIALLVVTLLSIFLIFLKQPSK
                     QQSDSKASTVETTTTSSVITSESSEVQTEIIWEQQTEPIKLPILMYHAIHVMAPEEEA
                     NANLIVDPTTFESHLKALQDAGYYTLSPEEAYKVLTENVLPAGKKVVWLTFDDSLWDF
                     YSYAYPLLKQYQMKATNNVITGFTEYGQAGHLTLDQIKEMQTAGLSFQGHTVNHPDLE
                     YSSIEDQTNELTSSKAYLDSQLNQETIAIAYPGGRYSADTVALTEQAGYKLGVTTNNG
                     LASASDGLLTLNRVRILPTTTAEGLLSEISY"
     gene            complement(17152..18921)
                     /locus_tag="AC1PIW_000600"
     CDS             complement(17152..18921)
                     /locus_tag="AC1PIW_000600"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6605560.1"
                     /translation="MKNRSVFTRVWDYLRRYKSSVFLAVFLKTISAVMNALEPFVLGL
                     IITELTKNLLDIANGVEGAQINYSYIAIMLAIYALRALLYEIGAYGSNYFMTKAVQGA
                     TKELRQDLSHKINKIPVSYFDKHQYGDLLGRFTSDVETVSNALQQSFLQLVNAVLTLS
                     LAIFMCFWLDVSLALVVVTLVPVTYFGSKFVMGKSQPYFKQQADALGRLNGFVQENLT
                     GFNVLKLYGREEISTEEFRQITADLQEVGFKASFMSGLLMPLVHGFSNIAYVVVALLS
                     GLKVLAGTLTVGNMQAFVQYVWQISQPVQTLTQLAPQLQSAKSSLERIFSVLDELDEE
                     DANALELTAGLTGQVSFEQVEFGYSEDKPLIRNFNLDVKPGEMVAIVGPTGAGKTTLI
                     NLLMRFYDVTSGAIKVDGQDIQNLSRQSYRSQFGMVLQDAWLYEGTIKENLRFGRLDA
                     TDEEIVEAAKAANVDHFIRTLPGGYNMEMNQESSNISQGQKQLLTIARALLADPAILI
                     LDEATSSVDTRLELLIQKAMKRLMKGRTSFVIAHRLSTIQEADKILVLKDGQIIEQGN
                     HESLLADKGFYYNLYQSQFSESK"
     gene            complement(18930..20666)
                     /locus_tag="AC1PIW_000601"
     CDS             complement(18930..20666)
                     /locus_tag="AC1PIW_000601"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6605561.1"
                     /translation="MNLIWTYLKKYPKWIALDLLGAFLFVVVNLGLPTFLARMIDQGI
                     TKNDVSQLYFWAGMMGLIVLLGIVGRVTLAYAAGKLTTNIVKDIRNDLYEKIQDYSHH
                     EYEQIGVSSLVARMTNDAFVLMQFSEMVLKLGIITPLMMIASVIMTLVTSPSLAWTVA
                     VAMPFLVFVIFYVATKTRPLSEKQQKRLDTINQYVRENLMGLRVIRAFTREEFQEERF
                     ESVNEEYTDISKKLFNLTGLTEPLFVQIIIAMIVAIVWFALDPLKTGSLQIGDLVAFI
                     EYSFHALFSFLLFANLFNMYPRMSVSSQRIQEVLDMPISISKNEDGITETDTRGYLEF
                     ENVTFAYPGETESPVLHNISFKAKPGETIAFIGSTGSGKSSLVNLIPRFYDVTLGRIL
                     VDGVDVRRYNLKALRSKIGFIPQKALLFTGTIAENLKYGKMDASLAELHDATDVAQAK
                     EFIESKEEKFDTHLAEGGSNLSGGQKQRLSIARAIVKQPDIYIFDDSFSALDYKTDAI
                     LRSRLKEVTENATVLIVAQRVGTIMDADQIIVLNEGEIVGRGTHNELMESNEIYREIA
                     NSQLNRQSLTEE"
     gene            complement(20742..20984)
                     /locus_tag="AC1PIW_000602"
     CDS             complement(20742..20984)
                     /locus_tag="AC1PIW_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HIP6605562.1"
                     /translation="MDMIENLIIAIVKPLISQPDSLTIKIVDTPEFLEYHLDLDQSDI
                     GRIIGRKGRTISAIRTIVYSVPTSDKKVRLVIDEKE"
     gene            complement(21006..21278)
                     /gene="rpsP"
                     /locus_tag="AC1PIW_000603"
     CDS             complement(21006..21278)
                     /gene="rpsP"
                     /locus_tag="AC1PIW_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIP6605563.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRAPRDGRFIETVGTYNPLL
                     AENSVTLKEERVLEWLAKGAQPSDTVRSLLSNAGVLKKFHEQKFSK"
     gene            complement(21402..21710)
                     /locus_tag="AC1PIW_000604"
     CDS             complement(21402..21710)
                     /locus_tag="AC1PIW_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HIP6605564.1"
                     /translation="MKNIMEARKRVEEMEKIFNRQLELNQSLSDSMAQLNQEQSTYLQ
                     LLDYYQSQTYMEDLDLSNKGYFNGIPCGVLSEDGVYNLLFDRTNLASQLRELADMLEQ
                     "
     gene            complement(21710..22357)
                     /gene="pcp"
                     /locus_tag="AC1PIW_000605"
     CDS             complement(21710..22357)
                     /gene="pcp"
                     /locus_tag="AC1PIW_000605"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HIP6605565.1"
                     /translation="MKIIVTGFDPFGGEPINPALETIKSLPKTIAGAEIILVEIPTVF
                     DKAADVLEEKMAEHLPDAVLCIGQAGGRVDLTPERIAINQDDARIPDNEGQQPIDRTI
                     REDGQPAYFSTLPIKAMVEAIHRIGLPASVSNTAGTFVCNHLMYQALYLAEKQFPKTK
                     AGFLHIPFLPEQVVDKPGLASMSLNDIVRGVEVAIGAIVEYRDKEDIKKGGGSTH"
     gene            complement(22354..>23027)
                     /locus_tag="AC1PIW_000606"
     CDS             complement(22354..>23027)
                     /locus_tag="AC1PIW_000606"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIP6605566.1"
                     /translation="KERKKQSVGKMLGTYEAVLVLYKDKLILEQRGGQVLFFHPDTAM
                     LRIKSGRDPLLELLGKEKQSIIDCTMGLGSDSIVLASAGHRVTALESSKLVHFIVSRG
                     LQDFDSGLQEVNRAMKSIQTIWTDSLTYLKGQIDKSVDVIYFDPMFSEEIKESQNLSG
                     LSTLADRSRLTEEIVSEAKRVARKKLIIKAHFRDQVFEEFGFKRHVRPNQKFHYGEII
                     LEEEV"
BASE COUNT         6887 a         5070 c         4407 g         6663 t
ORIGIN      
        1 ggttcttagg atggcaaacc tcacgaccaa aataaatcat agcttgatga gcagggagcc
       61 agagttcagg aggtaagact tccatgactc gtttttctgt ttccaggggt gtagctgatt
      121 ttttgacaat atcgtggtgt ttacaaatcc gtcccacgtg ggtatcaact gcaaaggcgg
      181 gaattccgaa accgacactt aagacaacat tggccgtttt tcttcccact cctgccaagg
      241 cttctaattc ctcacgagtt tgagggacaa tgccattatg ccgttccatc aactgctggg
      301 cacagtcttt taaaaacttt gccttgtttc ggtacaaacc caaacgagaa atataaggct
      361 caatatcctt cacttctgct gccgccatgg cttggggcgt agggaaagct gcgaacaaac
      421 caggtgttgc cttatttacc gcagcatctg tagtttgagc agataagagc actgcacata
      481 ccaactcaaa gtgatttcga aagtccaagc taggcttagc atcaggatat agagcgatga
      541 tttcttcaat aacttttctt gcacgttttt ttgataatac catgaattca ttatagcatg
      601 aaaacttgga tttatctaaa atctggattg tcataaacct cattcctcgt ggtataataa
      661 aaagaaaagg gggaatatgt atggacttac aagaactctg tagaaaggtc attcaagcaa
      721 gaccacgcaa cagccataag ggaagctatg gtcgagtctt gctgattggt ggactgtatc
      781 cctatggtgg agcgattatc atggccgcac tagcaagcgt caacagtgga gcaggcttgg
      841 taactgttgc tactgaaaaa gataacatac cagccttaca tagtcatctg cctgaagcca
      901 tggcattttc atttgacgat caagccttac tgataagtag cttagaaaat gctgacttgg
      961 tattgattgg tccaggtttt ggggaaaata gcagagctga acagttattg gactttgttt
     1021 ttgaacattt atctgaccag cagattttag ttcttgacgg ctcagccctt accttagtcg
     1081 caaagcaaaa cagaaagaac ttcccgtgta aacaagtcat cctgacaccc caccagaaag
     1141 aatgggaacg gttatcagcc attccggttg ctgaccaaac aagccaaaga aatcttgaag
     1201 ccttgcaatc ttttcaagaa aatacaatcc ttgtagctaa aagttccgct actcaaatcc
     1261 taaaaaccca gccaaaccaa gtcaccccaa taggagctgg tggtccttac caagcaacag
     1321 gaggtatggg agatactctt gcaggtatga ttgcaggatt tgctgtacaa tttccccttg
     1381 ttcaactata tgaaagaatc cttgtagcaa cctacctcca ctcctatatt gccgaccaac
     1441 tggctcaaga ttattatctt gtaaagccaa ctgatgtctc aagaaacatc caaaagacaa
     1501 tgcttcaatt tcaaacacaa acaaccgcct aatcaatgat taagcggtct tttatataac
     1561 aaactcgtga ttaaagcctg acaaatggta gaaaatgata ctcaatgcaa atcaaaagta
     1621 gcctaggaaa cgaagccgaa gatagaactg gagttcatca tggcaagttg acaacggata
     1681 attttgattt tcgaagagta tgactatatt ttcttagact tcttctttat gacgtcgttt
     1741 gcttaccaac agagtcccta gcccaataat gacaccaaga acaagatagt atctcgtatc
     1801 attgtctcca gtatttggta gttgagttac ctgtttttct tgattagggc tagatagact
     1861 agcttgcaaa gtgactggac gaggtaaagt tggttgaggg tttggcttct gaccgatcga
     1921 tacatgccct gaattcccag tatcttttac aggaggtgcc tgtacatcaa ctttttctga
     1981 gtctataggt ggaggcgtta cagtgccttc atcttttttg tcagtggcag tatttgctgg
     2041 ctctttggca tcatttgttg aagtagcaat accattcagt tctgcctgcg cgcttgtcag
     2101 aacggctaga gcttgatcga cacttgcttg gcttgcagat tcatcagcta gaatcgcttt
     2161 cgctgcttca actgcctggt cataagcttg tttcacgttt tcagatgcat tgagatactt
     2221 agcatctgtc gcttttaata cggaagaaac ggatactgcg ctacgaaggg ctgaaatatc
     2281 agtcttttga ccatctaagt tggctttcgc cgctaacaag tccgccaacg cttggttgac
     2341 aactgcctgt gttacattct cttggttcaa tacaagttgt gctgaacgaa cagctgtatc
     2401 ataggcaatt tgtttggtat tttcagcatt gtaataaata aagtttgcct gagtcggtgt
     2461 gtagtttgat acttcagcaa ttagtttttc cttatcggta gcctgaccgt ttagctgctc
     2521 tctcgctgcc acaagattgg caagtgctga atctacttgt acctgattgt gtttgttggc
     2581 atatactgca ttggcatctt ctaatgcttt agcataggca gtttgaagtt ctggactagc
     2641 attgtaataa cgcgctgttt cttcaagtcc cgcaagttca gcaatcagtt ctttcaaagc
     2701 aactttatta atcaaggtta atttcttaac atttgactgg tttgcaagta ctgctacgtc
     2761 taattcttcc aaaaattcat agccttctgg gagggttgga aggatagtgt aggtaccaac
     2821 tggtacaaat ttaccataat cggtcttaga atatttagca tttggtagct gaatagtctg
     2881 gccatcagca gttaccagtt ggatggttga accagaaggt agtaatgcat caatatctac
     2941 cagcaagttg gccttatctt tcaaagtcac atagaaattc acctcggcag tgctattttc
     3001 ttccgaaatc gtcacgactg ctttggtttc acctgctagg cttgaccatt ctgtatcata
     3061 taagaataaa tcaaaggtgt aagtaccaaa tggtagctta agaacgctag tatcgccggc
     3121 atctcgtggt aattctgcaa caatcttgcc tgtttcatcg gtgactgagt aagagaaaag
     3181 tattggtaca ggactatttg tatctttatc aagaatattg acagttacca accctttttc
     3241 attgccgata ctgatcagat tctctacttt ttcatagctg atgttaccag cataatcctc
     3301 tactgtgtag tagaattttg aaatatctgc aaggtcaaga ggcaatgtaa aggagccatc
     3361 gtcgttttgt gccacaaata ccttgttatc agaaacggtt acatcaccat tttctaataa
     3421 ggaaggaata gttgtcacac cgcttgcatc cgctacaagg tagaatactt gctcgcgata
     3481 tagaccggat tctccttttt caatggctgg acgagggtta aatgtaaagt ttgtttcatc
     3541 ataggtagct gtggtgataa ctggtgattc tctatcaatg ataacgtcga aaatcatagt
     3601 ttgtactgct gcacctggaa cttcagatga gtaggtcaaa acgtattgat acttaccatc
     3661 tgctaaagca tttccctcgc tgtctgtccc attccattca gtagggtaaa taatgcttga
     3721 ttttggattt ttaggatcac cactatagaa gttcttattg cctgactgtg gttgactttc
     3781 ccaaagtgga tttgttcgtt cggtatcatc tgcagcatag acgcttgcga ctaaatccgt
     3841 ataatttctc aagaagacac ctttcaaagc gagcgaatct tggttgtcat ccccatttgg
     3901 cgagatagct aaatgaggct taccagactc atcgagctct aaaacaaagt atcctgcttc
     3961 atttttaaat gtacctagag tcttgatcgc aaagtcagat cgtttgtctg tagaatagat
     4021 taactccgta cttcctgtaa caagacctgt gtagtgatgg ctgatgtcaa cagtatctgg
     4081 ttgtgctgta acaggttcaa agtagaagcc ccccttacca tcggcaataa gattgtaaat
     4141 cggctcttct agaacggcta gattttggaa ttccccacgg aaaccaacgt atggaatgct
     4201 gacaatatca ccgacatcgg ctacatctgt aaatcgaaca aaaccttcaa gatagtaacc
     4261 gtttttcatt aaacctgtca attcttctgc aaagcttgct gcatcgacat taattgtaac
     4321 agttgtactt gaattggctt tcacagtcac cttaccgcca ggaatctctg ctaatagaca
     4381 cggagccaag gtaatcaatc cgttttgaac agtatccgtt gttaactgtg ttgagtagtt
     4441 taaagtctta tcttcgtttg taatgttatg aagtgtgacc gtaaagctga atatatcctc
     4501 aacatttccc aaggtaatgc tgccataacc gtcttcgcca gtcaaatata aacctgtaga
     4561 aatagctgcc gcagtatcga tgatacctgc accttgttgg cgaggggaag tgtaggctgt
     4621 tgtttctttg ttcacatgtg ctttagcagt agacataagc aagtgtttta ctaaggcttc
     4681 gatttcttga ggggacttag tagggtaagt tgcctgtaaa tattgtttta ctagtacggc
     4741 tgctcctgct acgtgtggtg aagccatact tgttccctgc atgttggcat agtcattatc
     4801 attgatggct gcataaatag caccacctgg agcagctaag tctggtttta gctcgccatc
     4861 cgctgataga ccccaacttg aaaaatctga aagaagacct gcttctgggt tagggcgaat
     4921 atctgtttcg ttattgaacg caatcttata tgagttagat gccaaagctt caccaaattc
     4981 caatggaatg aagatagatg gaattgcgat agctgtatca tccagttgca tgctcacatt
     5041 ggcttcacct gggcggctat tgaaaataac gacccctact gcccctgcag ctgttgcatt
     5101 ggctattttt tctgagaaac tgatggttcc tcgtttaata agcgcaagtt ttccaatcaa
     5161 atttaaacca tcaaaatccg aagcctgacc gattcctgca tagacatatt catattcctt
     5221 cccaatttca aatgatacag gacttttctc tggattatcg aacgaactct taccgtaatt
     5281 caaatcagca ttattttcta agccaatgat attaattact ttactgccaa ccgttgtgtt
     5341 attgtaagaa gcgacagaaa ttgcatcgcg agctgttgaa ggagctccga ccaagccata
     5401 atctggataa tcggctgacg gattagaatg acctgaacca aatgttccat cattaccagc
     5461 agcaataaca acagaaacac ctgcacgacg agcagcctcg attgctgcag tcacattttc
     5521 attcatgtta acaacagagc cgttagctcc tcccaggctg aggttgatgc tatctgcacc
     5581 taattttaca gcatcttcaa tcgcctttac gtacaatgct gcgcctgttg tagctttgag
     5641 gtctgagaat acacgcataa acatgacttg cgcttcagga gctacaccat acattaattg
     5701 tccagcgact ggttgtgtcg gatttcctgt ggcaatactc gttacgtgca taccgtgtga
     5761 acgtttgtct tcttctttca agacagtatt cacatcaaca tagttataac caaatacaac
     5821 cttatcgtta aaccattcgc cgtaggtaat tcctgctgct tctttagctg cttctatctc
     5881 tttttctgat ttatattttg cggttgagag atctgaaata tgcaatacat cgtggtcaac
     5941 gtcaagacct gaatctatga ttgcaactac tgttccttcg cccttgtaac cactatccca
     6001 aactttaggt actgtaatga ttgtattact atcaatgttt tgtggtttcg cttcttcctg
     6061 attagtaata gcagtagtct catctgctac ggcaagcttc tcctcttctt ctactttttt
     6121 ctctgaaata gctggagttt ctacagctgg ttgagtcgct tctacttctg tatttgtgat
     6181 gttttcaaga gcttctgagc caagtgtttc ctctacatta tctgtagtgg ttacatcagt
     6241 tggggtagcc tccacagaag tgtcaactaa ctcctcgaca acgggacttt ctgaggctga
     6301 ttcgctgacg attacgtcag gaacagttgc ttccacctta gtctctacaa ggcttgtcaa
     6361 ttcatctgca gctaccgatg gtgcacctgc aaacaaaaaa actgcaccca aaagaacaga
     6421 cacagttcca attttatact tccgaagtga gaaagtttct ttctttttca taacttttcc
     6481 tcctagtttt tctcctttat tattggcaaa gccaataaat atattaaaac ataaaattat
     6541 cagcattgca agtatattct gaaaattcag ttatttattc aaactataac tagctatcaa
     6601 taatatctat aaccatagat acagataata cacactaaaa agggagtgag taagacatcc
     6661 atgatggctc cgtccttacc cacagaataa tgagaatgag cataaagccc tcactaagat
     6721 tatataccta gtgagggcct tgtggttggt gcattattga tgttaattat gatgacaaaa
     6781 ctgctccacc ctactgataa gaaccacaat atgcgctcaa gaatatgact aaaattcagt
     6841 agataggctc tctaatttta agaaggaaag aattgtcata gtaatcatgt gtagatcaag
     6901 tcgatattgt cgtttgtatt cccctcaact tgtccaaagc gttacttagc tacatttgat
     6961 acaacgacta aaggtctacg tgcttaccac gattttctca gtcaatttca tgtagaagct
     7021 gttggtatgg aaagcacagg tgtctattgg cgacttgtct ggcatgctct atgtgatgac
     7081 ttcgagctaa tacttgctca accagcccac atgaaggcta ttccaggtca gaaaaccgac
     7141 aagaaggacg ctcactggat tgccaaatta acacggattg gtctgcttcc tcggagtttc
     7201 gttcccgatg aaaccattca agaattgagg gagttgacca gacaacgaaa acagtataat
     7261 attgggactt ttctcatctc tttgttaaga caatctaaaa aatgctaatc tccctcaaat
     7321 atcctacgaa cggtatgaat ttgagagatt ggaactgcaa cgagcaagga atcccccata
     7381 gaaagttggc tatttccttt cacaatcctt gacttaccgt gatgaatttg actggtgatc
     7441 aagcatgaat ccggcaaacg taactcgcta acaaatcttc tgtccaatgt ttcgttgaca
     7501 acaacctcta ttaaggtcat gtgttcttgc tgttctactt cttctggcag aattttttcc
     7561 aacattgctt catagactgg cgcgccacct aagaggtcca tcatcacata ggaactcaaa
     7621 gctaccaacc cgatgaccat caattggttc aaacttccaa ccatttcagt tactaaaata
     7681 atagcagtta gaggtgcttt ggaaatcgct gcgaaaaagc ctgccattcc caatacaata
     7741 aacaatagca tctgctcctg actgatgaaa ccaatatcaa ggaagaaccg tgcgacaaaa
     7801 agcccaagga gacttcctaa ggttaatatg ggtaaaaaga tgccccctgg tagaccagaa
     7861 ccataggaaa tcatgctcca tacaaatcgt attaaaatca agattgccaa tgtcagtagt
     7921 ccttgttcca aatatggaag ctctaaaatc aaatgatgcc cgccacccaa cagattaggg
     7981 aaaaaataac caatcggtat aataaacaaa aaggcaaata cactgtagta tggaactggg
     8041 agaggaatga tttttgagag gagggtatag aaatcttgta tattgaggat aacaatttca
     8101 tacacatatc cagcaaggcc aaggaaaact ccaagtaaga tatagatcca atattggtca
     8161 agagatagta actgtaagtc cttcggcata tgcaaaacag gcattaatcc aaaaacatgt
     8221 aaggaaataa agttcgcagt gatactggct gccagggcgg agacccatac cagacgtgaa
     8281 aactgatgat agacttcctc cacaacaaat aaaaggcctg ctattggtgc attgaaagct
     8341 gctgccaaac ctgccgcagc gccactagca atcaaactcc ttctttccat ttcactggat
     8401 ttaagaagaa ctgatacacc tttagccgtt acagctccta attgaatact gggaccttca
     8461 cgccccagca ttagaccaga tgcaatggat aaaacaccgc cgataaattt tctccaaagg
     8521 actgaccacc agttcaaatc cataattcct tttagttctg cctccacctg aggtatacca
     8581 gaaccttttg catttggctc gtctttgatc aacttgccaa caagtaaagc tatgaccaga
     8641 taaagccctc caataagaag taaatagatt gcattgtctg ttgccagaga atagaggtgc
     8701 gtaaagacag aaaatagttt ttcaatggct aagcgaaatg caacgaccac taaaccagcg
     8761 acaatgccaa ctaaactacc acgcagaacc gaatagagga ttgattgaga aataaaacta
     8821 acttctttcc ggtgattttc cattgggatt tcccttcctt tctatagtat gcatgaccaa
     8881 aaaataggag caataagtac ttagtaaaga acctcactcc cattctttta tttatattgc
     8941 tacctgtcca atcagactat ttccgataca tcttgaattg gagatagagg tcattataca
     9001 ttccaagcat ctctggtcca agattatcat aaacttccat cttcttcatc gtttcttctg
     9061 aaggatagaa ggattcgtcg ttctgagtct cctcatccag catagccttg gctgcaggaa
     9121 ttggtgttga gtagccaaca tattctgcat tgcgaagcgc attttcagga cgaagcataa
     9181 aactcataaa ggcgtaagct ccatcaatat tttttgctgt ttttggaatg accatgttat
     9241 caaaccacag gttagaacct tttgatggta caacatagcg cagattttca tttccatcta
     9301 acatttcact cgcctcacct gagaaagaaa cagcaattgc tgccgcatct tgtatcatat
     9361 aaccttttat ctcatcagca acaattgctt tgacatttgg agttaaatta taaagatgtt
     9421 cggctgcctt ttcaatctcg gtaggattgt tagaattgag actatagccc agtgattgta
     9481 gaccaaagcc catgacttca cgcacaccat caattaacat gatattgtca cggtattcct
     9541 ctgaccacaa gtcttcccat tcctgaggag gattttctac catggtttca ttgtaaacaa
     9601 tgcccaaggt tccccagaaa tatggaatag aatagtcatt attcttatca aagctaaggc
     9661 ccataaatcg tggatcgata ttttctatcc cttcaatctt agacttatct agcttgatta
     9721 acatattttc ttctgtcatc ttagaaatca tgtactcaga tgggattgtc aaatcgtatg
     9781 ttgttccacc ctgcttaatc tttgtgtaca tcgattcgtt ggagtcaaag gtctgataat
     9841 ctacctgaat acctgtttca gcagtgaatt ctgcgagtaa ttctgggtca atataatcgc
     9901 cccagttata gatgacaagt ttatcagttg tcccttgggt tgaatcagct tctaacttat
     9961 aactaattcc ccataaaact aggacaattg ctataattcc tacaaaaaat gaatacaatc
    10021 gtttcattag ttggcctcct tttcacgtga gatgaaataa taacccacta ccaataagat
    10081 agagaaaagg aaaactaggg ttgacaaggc attgatttct agagaaatcc cttgacgagc
    10141 ccgtgagtaa atctctacag ataggtttga gtaaccattt cccgtaacaa agaatgtcac
    10201 ggcaaagtca tccaaggaat aggtaaaggc cataaagtag cctgcaatga tggctggtgt
    10261 taggtatggc aacatgattt ctttcagcat ttgtggctga gttgccccca aatcataagc
    10321 tgctgaaatc atatctgcgt tcatctcttt cagccgtggc aagaccatca atactacgat
    10381 tggaatagaa gaagcaatat gactgagcaa tactgagaca aagccaagct ggaaaccaat
    10441 catggtaaag agaattagga aactcgcccc aatcatgacg tctggagcta ccatcaagat
    10501 attattgaca gacaagagtg cattttgaaa acgaggtttt gcctgataga tatagatggc
    10561 accgaaagtt ccgataatgg tcgcaatcaa agaactcaag aaggccaaca agaaggtttg
    10621 agccagaatc agcattagtc ggctatcgcc aagcatagat gaaaaatgct ctaaggtaaa
    10681 gcctgtaaag ccattcatat ctccgccttc attaaaggca taaaaaataa gatagaaaat
    10741 cggtaagtag agaaggagaa aggcaactgt taagtagata tttgcaaatt tcttcattat
    10801 ttcttcctct ctttcgtcat ccacataatc aagagcatgg ccaaaatcaa gaccacacct
    10861 atggttgaac ccattcccca gttttgtgtt gtgagaaagt gttgttcgat agctgtacca
    10921 agcgtgatca ctcggttgcc gccaatcaaa cgggtcaaca tgaagaggct cagacttgga
    10981 atgaaaaccg cctgtacacc agaacggaca ccattcatag aaagaggaaa aataactttt
    11041 gtgaaggtct gccaaggagt tgcccccaaa tcacgactgg cattgattag gtttttatcc
    11101 aaatcatcca aggcgttaaa gatcggcaaa atcataaacg gaatctcaat ataggctgca
    11161 acagcaataa aggagaaatc tgtgaaaaga atttgctgag ctcctagacc aataaattct
    11221 aggaactggt tgatagagcc gtgttgtcca aaaataccga taaaggcata ggctttcagc
    11281 aaaagattga cccaggttgg caagataatc aacatgagcc agagctgtct atgcttgagt
    11341 ttggtcaaga aataggctgt tgggtaggaa accaaaagcg tgaccagagt aataatacct
    11401 gcgtaaaaaa tggagttaaa actcattcgc agataggtca tatttggcga gttaaaatag
    11461 gtctggtaat tggccaaagt gaaatcgcca tgaatatcaa agaaagattt gaagataatc
    11521 aaggcaactg gagcgagtac aaatagaaag acccataggg catagggaat tgcaaaaagc
    11581 ctagaggttt tcttcattgc gttcctcctc aatggcgtta atcaagccat cttctacttc
    11641 ttccacttcc acatattctt cgatacgggc atcgaattct tcctctgttt cattgagacg
    11701 catgatatga atatcttctg gctcaaaact tagtccaatc acttctccga caatggcctt
    11761 acgagttgag tgaatcatcc actcattacc gaggtcgtca taggcgatga tttcatagtg
    11821 aacccctcgg aagagctgag tatccacttt tacctgcaat ttgccttcct caggtagggt
    11881 aatctgcaaa tcttctggac ggataacgac ttcaacttct tcattaggac gcataccgcc
    11941 atcgacagct tcaaagcgtt tgccgttaaa ctcaaccaga tagtcttcta tcatgcgacc
    12001 tggaaggata ttggattcac cgatgaaggt agcaacaaaa tggttgattg gttcgtcgta
    12061 aatgtccact ggcgtcccag actgaacaat ttcaccttca ttcatgacaa aaatccagtc
    12121 actcatggcc agagcttctt cttggtcatg ggtaacgaag acgaaggtaa ttcctagacg
    12181 ttgttgcaat tcacgcagtt cgtactgcat ttctgttctt agtttcaagt ccagggcaga
    12241 aagtggttca tctagcaaga caacgcgagg ctggttgata atcgcacgtg caatagcaac
    12301 ccgctggcgt tgaccaccag acaatttctg aatggaacga ttttcataac cagaaagtcg
    12361 gaccatttgt aaggcttctg tcacacggcg ttcaatttca gccttgtcaa tctttcgtaa
    12421 tttaagagga aaagccacat tttcaaagac agtcatatgt gggaaaaggg cataggactg
    12481 gaaaacagta tggacatccc gtttattggt cggaacatca ttaatccgtt gaccgtccag
    12541 ataaatatca cctgaactag catccaataa accagcaata atattcaaaa tcgttgattt
    12601 acctgaacca gaagctccta aaagggtata aaatttcccc tcttccaatt caaaactaat
    12661 atcctttaat acagttgttc cactgtcttc aaatactttt gaaacgttct taaattcaat
    12721 aattggcttt ttcaattctc ataaattcct tcttttttgt aagagcctga tggctagagt
    12781 gtatcagcag gatcgccgat aatacgaacc tctctctcca gagtaattcc tgaagatttt
    12841 tcaacaactt gaatcacatg ggcaatcaaa ttttcgtaat cagatgctgt accattttct
    12901 atattgacca taaagccagc gtgtttttca gaaacttcga ctccaccaat tcgataacct
    12961 tttaaacccg catccataat caactgtcct gcgaagtgac caagtggacg cttgaagact
    13021 gaaccacaag atggatattc caaaggttgc ttgagctcgc gcaaatgtgt caaacgagcc
    13081 atttcttgtt gaataaccgt atgatttcct ggcctgaggg caaattttgc agacaaaaca
    13141 atcgcaccgt tttcctgcaa aatagaagaa cggtagccaa atcgtaactc acgattgtcc
    13201 aatgtctcga cataacctgc aggagtcaaa atctgcgctg aaactaaaat gtgtgccact
    13261 tcacctccgt aagcacctgc attcatatag acagcaccac caatgcttcc cggaatacca
    13321 caagcaaatt caaatcctga taacgaatgg aaaagtgcga cttttgttgt ttcaatcaga
    13381 ttggcacctg cttctgcttc aatggtgtat cctgatacag taacggagtt aagtttatcc
    13441 atacggataa caaaaccacg aataccgcca tcgcggacaa tgatattact tgaatttcct
    13501 aatacttgcc atggaatacc ttcgcgttta gcaaattcta ctatacgaac aatctcgtaa
    13561 cgattccgcg gaaaagctag ataatcaaca gctcctccta cttttgtata ggtatattct
    13621 tttaaaggtt catcgaatcg aatatcgatg ccttctaatt caagcctaat tttatctttc
    13681 ataaccttct catttcaatt taagatatac aggatttatt ataccaaaaa ttacaggaga
    13741 aaacgaataa aattcaacaa aaatactttc acaagccaaa ttaatttgcc ccaatatttt
    13801 gaaatggaga atggaatgca caaaaaaaag gagttccctt tctaccgctt acagtagcga
    13861 tggcgaactc caataaataa acatgtttag ttttgcaaat gacagattct cactatctat
    13921 acaatagcaa tccctttggt atctacttga aggcggtgga cagttccgtc tagatttagt
    13981 tcctctaatg ctagaacaat agcgtcctcc ctatctttgg gcgctaatac catcactgtt
    14041 ggaccggcac ctgataagta ggttgcatag gctcccaact cctgtgcagt ttgcttgatt
    14101 ggacaaaact ccttaacaag tagctgacga aacggttcat ggaacatgtc tgactgaatt
    14161 gctttcccag ctttttccag atcacctgcc atcaaactgg caacagcaac gtttgcaatg
    14221 gcactagcag ccactgcttt cttgtacccc atctgagaag gcagtacacc tcggctttca
    14281 accgtacgca gtgggtagtt tggaataaag gcaacaaaac ttgcttctgg gaattctgtt
    14341 acaactgcct gtactctctt attaacatag ctagagatga ctaaattacc gaaaatcgct
    14401 ggggctacat tatcaggatg tccttcaatt tctgtagctt tgagcaattt ttcatccgct
    14461 gtcatattca aacctgctaa ttgattagcc aattcgatac cagctacgat aacggaactg
    14521 gatgaaccca aaccacgtgc caagggaata tcactaatca ttctgatacg atgcggctgc
    14581 aatccttttt caatctttaa agctgtcttg atcaaaagat tgttcttgtc tgatggaaca
    14641 tgttcaagat tgtgctcaat cacccattca tcagtctctt caaatacttc aatcgttaga
    14701 tatttcgaaa gggcaacacc tactgaatca aatcctggac cgatattagc tgaagttgct
    14761 ggaataataa ttttcattgc ttattctccc agtactttaa aggtatttaa gacagtaaag
    14821 tctgtttctt tactcaattt gtctgttaca ttttccaact gagtcttact catttcatgg
    14881 gtcacaatca ctaggcgggc tgtttctcca tctgcagctt gttgcagaat ttgcttgaag
    14941 gaaatttctt cagaattgaa gatttctgct aaacgaagca tttttccatt tttatcaggc
    15001 gcatcaattg agaaataata ttcgctcttc acatcgcttg gggctgccaa ttgaagtggg
    15061 cggctatatt cgttaaaagc tttgccgata gtattgtctt tcaagcgacg aacgatacga
    15121 ataatgtcag ccataacact tgttgcagtc ggtttttgcc cagcacccgg tccatagtac
    15181 atagactggc cgatgccgat agattcgaca aatacagcat tcatcacacc attcacgctt
    15241 gcaagtggat gatttttcgg taggaaggtt ggagatactt cggcagcaat accagaggta
    15301 gtttcttgaa tatcacccac aagtttgatg acatagccca attcttgtgc cactgctaca
    15361 tcttctggtg tgatattcga aatacccttg tgtgctacgt gttcaaaatc aactgtcatc
    15421 ccgaaagcaa attgactgag gataacagcc ttgtaagctg catcaatccc ctcaacgtca
    15481 tttgttgggt cagattctgc atagccaagt tcctgtgctg tttgaagagc tttttcgtaa
    15541 gtccagcctt catcaatcat cttagtcaac atgaagtttg aagtaccatt caatacaccg
    15601 aggatacgag tcaccttgtc agcagccaat gagttaacca aggtacgcaa gattggaata
    15661 ccacctgcaa ctgcagcttc ataataaagc gcaagacctt tctcttcagc caaggcacgc
    15721 aattcgctac catgaacagc aagtaaatcc ttattggctg ttacaatatg cttgcctgct
    15781 tccaaggcat gtgtaataaa tgtttttgct ggctcaatac gtcccatcag ctcaacgacg
    15841 atggcaatgt cctcatcagc taaaatatca tcaatatttg ttacaaaatg atagtcatga
    15901 cctgcagcca agagacggtc tttttctgct tcatccttga ccaaaacctt agcaacttca
    15961 atactatcat gcgcagcttt ctctattttt tcctgatttt cttctaataa aaatggcaca
    16021 ccactcgcaa cggttccaaa tcctaataat cctatcttaa ctaccatgtg ctctccttta
    16081 aaatgtattt ctcccattat aacaaatatt ctgactattt ggcaaaaaat ttgatataat
    16141 tgttttaata tatgattatg gagataagat aatgaaacat tcaagaagag cccgtcgaag
    16201 ccgcacacaa aagaaacaac ttgtgccaat gctacttggc atcgcattat tagtagtgac
    16261 tctactatct atttttctaa tctttctaaa acagccatct aagcaacaaa gtgattccaa
    16321 agcttcaacg gtagagacaa caaccacttc aagtgttata acttcagaaa gtagtgaagt
    16381 acaaacagaa atcatttggg aacaacaaac tgaacctatc aaacttccaa tcctcatgta
    16441 tcatgccatt cacgtaatgg ctcctgagga agaagctaat gcgaatctta tcgtagatcc
    16501 aacgactttc gaaagtcatc taaaagcatt gcaagatgca ggttactata ctttatcacc
    16561 tgaagaggct tataaggtat tgacagaaaa tgtccttcct gctggcaaaa aggttgtctg
    16621 gctaaccttt gacgatagcc tctgggattt ttatagttat gcctacccat tgcttaagca
    16681 atatcaaatg aaagcaacca acaatgtcat tacaggattt actgaatatg gacaagcagg
    16741 gcaccttact cttgatcaaa tcaaggaaat gcagaccgca gggctctcct ttcaagggca
    16801 taccgtcaac caccctgatt tagagtatag ttctatcgag gatcaaacaa atgaacttac
    16861 ttcctctaaa gcctatcttg acagccaatt aaatcaggaa accatcgcta tcgcctaccc
    16921 tggtggacga tactcagcag ataccgtagc cttaactgaa caagctggct acaaacttgg
    16981 tgtgactact aacaacggct tggcttctgc tagtgacgga ttactgactc tcaatcgtgt
    17041 ccgcatcctc ccaaccacta ccgctgaggg actattatcc gaaatttcct attaattttc
    17101 acaaacatgt atatgagaaa actccctgtc actgacaagg gagttcgttt tttatttgga
    17161 ttcactaaac tggctttgat aaagattata atagaaacct ttgtctgcca aaagactttc
    17221 atggttacct tgttcgataa tctgtccatc tttcaagacc aaaatcttat cagcttcctg
    17281 aatggtagat aagcgatgag cgatgacgaa acttgtccgc cctttcatca accgtttcat
    17341 ggccttttga atcaagagtt ccaaacgggt atcaactgaa gaagtcgctt cgtcaagaat
    17401 cagaatagca ggatccgcca acaaggcacg tgcaatggtc aaaagctgtt tctgaccttg
    17461 ggaaatattg ctggattcct ggttcatttc catattataa ccgccaggca gggtacggat
    17521 aaagtggtca acgttggcag ctttagcagc ctccacaatt tcctcatccg tcgcatccag
    17581 acgtccaaaa cgtaggtttt ccttgatggt cccttcatag agccaagcat cctgcaagac
    17641 cataccaaat tgactacggt aggattgacg tgaaagattt tgaatatcct gcccatctac
    17701 cttgatagca cctgatgtga catcatagaa acgcatgagc aagttgataa gggttgtctt
    17761 acctgcccct gttggaccaa cgatagcaac catttcacct ggcttaacat caagattaaa
    17821 gttacggata agcggcttgt cttctgaata gccaaattcg acctgttcaa agctgacttg
    17881 tccggtcaaa ccagctgtca actccagcgc attggcgtct tcttcatcaa gctcatctag
    17941 gactgaaaag atgcgttcaa gggaagattt ggcagactgt aactgtggtg ccaactgggt
    18001 caaggtttgg actggctgag aaatctgcca gacgtattgg acgaaggcct gcatattacc
    18061 caccgtcaat gtacctgcaa gaactttcaa tccagatagc agggcgacga caacataggc
    18121 gatatttgag aagccgtgta ccaggggcat gagcaaacca gacatgaaac tagccttgaa
    18181 accaacttcc tgcaaatctg cggtaatctg gcgaaactct tctgtagaaa tttcttcacg
    18241 gccgtagagt ttgagaacgt taaaaccagt cagattttct tggacaaagc cgttcaaacg
    18301 acctagggca tcagcctgct gcttgaaata tggctgggat ttgcccatga caaacttaga
    18361 accaaaataa gtcactggaa ccaaggttac gactacaagt gctagggata catccaacca
    18421 gaaacacatg aaaatggcta gagagagggt cagaacggca ttgactagct ggagaaaact
    18481 ttgttgcagg gcgtttgaaa cagtctccac atcgcttgta aaacgtccga gtaaatcacc
    18541 atactggtgc ttatcaaagt aagaaacagg aattttgtta attttgtgac tcaaatcctg
    18601 acgtagttcc ttagttgctc cctgcactgc cttggtcata aaatagttag aaccgtaggc
    18661 accgatttca tatagaaggg cgcgaagggc atagatggca agcatgatgg caatgtagga
    18721 atagttaatc tgcgctcctt ctactccatt agcaatatcc aacagattct tggtcaattc
    18781 tgtaatgata agccccagta caaagggctc caaggcattc ataactgcac tgatggtctt
    18841 tagaaaaaca gctaaaaata cagaagattt gtaacgacgc aaatagtccc aaacacgggt
    18901 aaatactgat ctattcttca taattcctcc tattcttctg ttaacgattg acggttaagc
    18961 tgagaattgg cgatttctcg ataaatctca ttgctttcca tcaattcatt gtgggtgccg
    19021 cggccaacga tttcaccttc gttcagcacg ataatctggt cagcatccat aatggttccg
    19081 acacgttggg ccacaattag caccgtcgca ttttccgtca cttccttcaa acgactcctc
    19141 aaaatcgcat ccgtcttata atccaaggct gagaaagaat catcaaaaat gtaaatatcc
    19201 ggctgtttga caatagcacg tgcaatggac aaacgttgct tttgcccgcc tgacaggttg
    19261 cttccacctt ctgctagatg ggtgtcgaat ttttcttcct tactctcgat aaactcctta
    19321 gcctgagcca catccgtcgc atcatgaagt tctgcaagac tagcatccat cttaccgtac
    19381 tttaagtttt ccgcgatagt tccggtaaat agcagagctt tctgcggaat aaagccaatc
    19441 ttgctgcgga gtgcctttag attgtagcgt ctgacatcca caccgtccac caagatacga
    19501 ccaagtgtca catcatagaa acgtggaatc aagttgacca aggaagactt accagaacct
    19561 gttgaaccaa taaaggcaat cgtttcacca ggcttagctt tgaaggaaat attgtgcagc
    19621 acaggcgatt ccgtttcacc agggtaggca aaagttacat tttcaaattc caaataacca
    19681 cgagtatctg tttcagttat gccgtcttca ttcttagaaa tggaaatggg catgtccaag
    19741 acttcctgaa tccgttggct cgataccgac atacgtggat acatgttaaa caagttagca
    19801 aagagcaaga aagagaaaag cgcgtggaaa ctatattcga taaaggctac caaatcaccg
    19861 atttgcagac tgcctgtttt caagggatcc aaagcaaacc agacaatggc cacaatcata
    19921 gcaatgataa tctggacaaa gagaggctct gtcaatccag tcagattaaa gagctttttg
    19981 gaaatatcgg tatattcctc attgaccgac tcaaagcgct cctcttgaaa ctcctcacga
    20041 gtaaaggcac gaatgacacg taagcccatg agattttcac gaacatattg gttgatcgta
    20101 tccaaacgtt tttgttgttt ttcagagaga ggacgagttt ttgttgccac atagaaaata
    20161 acaaaaacta gaaacggcat ggctactgct actgtccagg ctaagctcgg actggttacc
    20221 aaggtcatga tgacagaagc aatcatcatc aagggggtaa taatacctag ttttaagacc
    20281 atttcagaga attgcatgag aacaaaagca tcatttgtca tacgagcaac tagagaagaa
    20341 acaccaatct gctcatattc atgatgagaa tagtcctgaa ttttctcata cagatcattt
    20401 cgaatatcct tgacgatatt tgtagttaac tttccagctg cataggcaag tgtcactcga
    20461 ccaacaatcc cgagcaggac aatcaatccc atcatccccg cccagaaata gagctgacta
    20521 acatcgttct ttgtaattcc ttggtcaatc atcctagcca aaaaagtcgg caagcctaag
    20581 tttaccacta caaataagaa ggcccccaac aagtctaagg caatccactt aggatatttt
    20641 ttcaaatagg tccaaatcag attcatcatt tcttcctttc tatatgttaa aaaggggcta
    20701 ggcccctttg tcaacgttaa aagccaccaa acggtgactt tctattcttt ctcgtcgatc
    20761 actaaacgaa cttttttatc acttgttgga acagaataga caatcgttct gattgcagaa
    20821 attgtgcgtc cttttctacc aatgatacgt ccaatatcag attgatccaa atccaaatga
    20881 tattccaaga attcaggtgt atcaacaatt tttattgtca agctatcagg ctgtgaaatc
    20941 aaaggtttca caatcgcaat aatgagattt tcaatcatgt ccataagtgt tctcgcttac
    21001 tgttcttact tagagaattt ttgttcgtgg aatttcttca atacgccagc gtttgaaaga
    21061 aggctacgaa ctgtatcaga tggttgtgca ccttttgcca accactcaag tacacgctct
    21121 tctttaagag ttactgagtt ttcagccaaa agtgggttgt aagtaccaac agtttcgatg
    21181 aaacgaccat cacgtggtgc acgtgagtct gcaacgttga tacggtagaa aggtttcttt
    21241 ttagaaccca tacgagtcaa acggattttt actgccattt ttaaatgttc tcttttcttt
    21301 gtatttttat tcggtgaaat agcagagcta tttagcacat gttctattat accacacttt
    21361 aacagggtgt caagaaaaaa acttgacagg ttttaaaaaa tttattgttc gagcatatct
    21421 gctagttctc tcagttgact tgctagattt gtacggtcaa acagtaaatt atagactcca
    21481 tcttcactca atactccgca aggaatacca ttgaaataac ccttattaga taaatcaaga
    21541 tcttccatat aggtctggct ctggtaataa tctaataact gtaaataagt cgattgttcc
    21601 tggttgagtt gtgccatact atcggaaagg gactggttca gctctagctg tctgttaaat
    21661 attttttcca tttcctctac acgttttctt gcttccataa tatttttcat cagtgtgtac
    21721 tgccaccacc ctttttaata tcctctttat ccctatattc aacaatcgct cctatagcta
    21781 cttcaactcc tcttacaata tcgttcaaag acatggaagc cagtcctggt ttgtcgacaa
    21841 cctgctctgg caagaaggga atatggagaa agcctgcctt agtctttgga aattgtttct
    21901 ccgccaagta aagagcttga tacatgagat ggttgcagac aaaggtccca gcagtattag
    21961 aaacagaagc tggaagccca atcctatgaa tagcttcaac cattgctttg atgggtaagg
    22021 tggagaaata agcaggctga ccatcttctc gaatcgttct atcaatcggc tgttgcccct
    22081 cattgtctgg aatgcgtgca tcatcttggt taatggctat acgctcagga gttaaatcaa
    22141 ctcgcccacc agcctgacca atacatagta ccgcatcagg taaatgttct gccattttct
    22201 cctctaaaac atccgcagcc ttgtcaaaaa cagtcggaat ctctacaaga ataatctctg
    22261 caccagcaat ggtcttaggt agagatttga ttgtttccaa ggctgggtta ataggctcgc
    22321 cgccaaaggg atcaaagcct gttacgatga ttttcatact tcctcctcca aaataatttc
    22381 tccgtagtga aatttttgat tgggtctgac atgacgttta aagccaaatt cttcaaagac
    22441 ctgatctcga aaatgagcct ttattatcaa tttcttccta gctactcgtt tagcctcaga
    22501 tacaatttcc tccgttaaac ggcttctgtc agccaaagta gacaagccag aaagattttg
    22561 tgattccttg atttcttctg aaaacatggg atcaaagtaa atgacatcaa cagacttatc
    22621 tatttgccct ttcaaataag tcaaactatc tgtccaaatt gtctgaatag atttcatggc
    22681 tcgattgacc tcttgcagcc cactatcaaa atcctgtaaa cctcgactca caataaagtg
    22741 cactagctta gaactttcca aggctgtcac ccgatgaccg gcacttgcta acacaatgct
    22801 atcagatccc aagcccatag tacagtctat tattgattgt ttttcttttc caagtaattc
    22861 taaaagcgga tctcttccag acttaatccg caacatggca gtatctgggt ggaaaaataa
    22921 aacctgccca cccctctgct ctagtatcaa tttatcctta taaagaacca agacagcctc
    22981 gtaggtacct aacatttttc caaccgactg cttctttctt tccttgt
//
LOCUS       DBNFCK010000020        22582 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.020, whole genome shotgun sequence.
ACCESSION   DBNFCK010000020 DBNFCK010000000
VERSION     DBNFCK010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 22582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22582
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.020"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            1..778
                     /locus_tag="AC1PIW_000607"
                     /pseudo
     CDS             1..778
                     /locus_tag="AC1PIW_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935295.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(987..1889)
                     /locus_tag="AC1PIW_000608"
     CDS             complement(987..1889)
                     /locus_tag="AC1PIW_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6605567.1"
                     /translation="MSKENKKQVQSASTPPGGFRVIAREFMKDRLALTSLIILVTVLL
                     AVFIGALLLNQEQVMKVNLLGRYLEPGVDGYILGTDEGGKDIFGQLIIGARNSIIIGF
                     TITIITSIVGISVGLISGYYGGRLDGFLMRIVDFIMILPVTMLIIVFVTVIKNYTIYH
                     FILIMSAFYWTAKARLFRTRTLSEASLDYVNASKTLGTSDFMIMFREILPNLSSLIIV
                     NLTLNFAGNIGIEASLTYLGFGLPSTVPSLGTLIANARNADILENKTWIWLPAAIFIL
                     VMMLCINYVGQAFQRAADAKQRLG"
     gene            complement(1903..2859)
                     /gene="opp4B"
                     /locus_tag="AC1PIW_000609"
     CDS             complement(1903..2859)
                     /gene="opp4B"
                     /locus_tag="AC1PIW_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HIP6605568.1"
                     /translation="MWKTVLRRLLMMIPQIIILSVIAFFVAKMMPGDPFTGLIDPNID
                     PAIIEQKRIAAGYYDPIPVQYFRWVGNLLQGDFGQSVIFKQPVVDVIMQRLNNTIGLS
                     LLTMVLTYLIALPLGMIAGRYQNSLADKIIGVYNFLTFSTPTFVFAILMLWMFGFSLG
                     WFPTRGSISGGVEGFAATLSRLHHMILPAITMAILSTTVTIQYLRTGIIDAKSQDYVR
                     TARAKGVPERVVYNRHIFRNSILPIASFLGYELTGLIGGSIFIENIFTYPGIGQLFFN
                     SISSRDYSVILALLLVFGMGTLLGTLISDIIMSIVDPRIRVK"
     gene            complement(2861..3802)
                     /locus_tag="AC1PIW_000610"
     CDS             complement(2861..3802)
                     /locus_tag="AC1PIW_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIP6605569.1"
                     /translation="MGFIEVKDLKVHYPIRSGFFNRVTDHVYAVDGVNIEFEEGKTYG
                     LVGESGSGKSTIGKAIIGLERATSGQIIYEGQDVTNKSRGKKGNFNRDVQMIFQDSLS
                     SFNPKKRILDIIAEPIRNFDRLSPDEEKKKVLQLLDTIGLNEEALIKYPHEFSGGQRQ
                     RIGVARALASNPRLIIADEPVSALDLSVQAQVLNYMKRIQDEFKLSYLFISHDLGVVQ
                     HMCDELFIMYRGRFVETGNKNDIYNNPQHIYTKRLLSAIPTIDPLNRLKNKEKRLAAE
                     KEYQEMQGQYYDENGRVYDLRSFSETHQVALPEGGQN"
     gene            complement(3805..4803)
                     /locus_tag="AC1PIW_000611"
     CDS             complement(3805..4803)
                     /locus_tag="AC1PIW_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6605570.1"
                     /translation="MATEKPLLDIKDLHVGFRIGDDYFDAVDGVDISLQKNEILAIVG
                     ESGCGKSTLATTIMGLHNPLNTKITGSIQYNDMELIGMDEAKYNTVRGNDIGMIFQDP
                     LASLNPLMTIGAQIDEALFYHTDLDANARTERVLELLAQVGIPNPKRTFKQYPHELSG
                     GMRQRIIIAMALSCKPPIIIADEPTTALDVTIQAQILDLLNDIQAETGSGIILITHDL
                     GVVAETADRVAVMYGGQFVEVAPVEELFTNPKHPYTRSLLKSNPQSGSEGGDLHVIEG
                     IVPPITKMVRKGCRFAPRIPWIGAEAHEENPSMHEVGPNHFVRCTCHETFYFEGEA"
     gene            complement(5180..6970)
                     /locus_tag="AC1PIW_000612"
     CDS             complement(5180..6970)
                     /locus_tag="AC1PIW_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HIP6605571.1"
                     /translation="MKKTTKLFALAGVTLLSASVLAACGSKQSGAAKQELSFPAEVKQ
                     DGTAVADAQLKYAWVSPTTSSGLLIDELTENTTDSTFGGMVDISMFGYDGERKLDDSG
                     LAKAEFDVKGKKITVSLTGKDYKWSDGESFTINDYIFTIKSMASKDYTGIRFDDKFLN
                     IEGMQEFVDGKASDISGIKKVDDYTVELTVKEMSPSMMYAGGDVPAYIQPEHIYKDIP
                     VADWEKSEYSRTAKLVGMGPWKIKEIVNGESITYVPNEYFFKGTKPKTSSLKIDIVSP
                     DTIVSEMKAGNYDIAEMPVDQLDSYKDASNLNIVGQLESSYEYISFNFGKYDEAAGKN
                     VMDENAKMNDVKLRQAIAYAIDTKTAGKSLYNGLYHPAKSLIISFFGDIHDSELEGYS
                     YNPEKAKKLLDEAGYKDVDGDGIREGKDGKAFKITFAARKRTEANEALVQQYIAWWKE
                     VGLNVELYTGRTVEGKSFYNSVQANDAAIDMYAGGWSTGYDPNPSGLWGPIAAFNMSR
                     FVSDENTKLLEAISSAESFDDKKNVENYKAWQKYAHEQAFAIPTFESEKITAVNKRVK
                     SYDTKYGSASGTGLAYEGIELTADKGVVAE"
     gene            7183..8424
                     /gene="pbp3"
                     /locus_tag="AC1PIW_000613"
     CDS             7183..8424
                     /gene="pbp3"
                     /locus_tag="AC1PIW_000613"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase PBP3"
                     /protein_id="HIP6605572.1"
                     /translation="MRKLLLTLTTILAVFLTSTPILAEDFSVAAKHAIAVEVSSGKIL
                     YEQDAETKASIASISKTLSIYLVYEALAKGEITLDTMVDISDYPYSLTSNTELSNVNL
                     DERSYSVRDLLNASLITSSNSAIIALAEKIAGSEAAFVDRMKAKVQEWGITDTKIVNV
                     SGLDNTDLGENIYPGSSPEDVNMFSALDVAIIARRLILDYPQVLDITSLNAYDFGGYT
                     YYSTNQMLSDGTHARGGVDGLKTGTSNSAGSSFLATTQQAGMRIITVVLNANDGLTSP
                     ENRFVATNDLMNYVYSNFSLVTLVKKSEAFGDSKIKVFNGEKETSPVVAAADLTVVQR
                     NQTDNVPTATFTTDIKEIDAPLKAGTLVSKLQLKDQDLIGKGYISEQTGVDMIIPSDM
                     KEASWPFSWWNQFVRYVNEKL"
     gene            complement(8677..10092)
                     /gene="sufB"
                     /locus_tag="AC1PIW_000614"
     CDS             complement(8677..10092)
                     /gene="sufB"
                     /locus_tag="AC1PIW_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HIP6605573.1"
                     /translation="MSEERIEPTPIDLGEYKFGFHDENVELVASTGKGLNEEVIREMS
                     RIKGEPEWMLEFRLKSYETFKKMPMQTWGADLSELDFDDIVYYQKPSDKPARSWDDVP
                     DKIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEYDKHGIIFTDTDTALKEHP
                     ELFKKYFGKLVPPSDNKLAALNSAVWSGGTFIYVPKGVKLDIPLQTYFRINNEGTGQF
                     ERTLIIVDEGASVHYVEGCTAPTYSTASLHAAIVEIIAHEGAYMRYTTIQNWSDNVYN
                     LVTKRARAEKNATVEWIDGNLGAKTTMKYPAVYLEGEGARGTMLSIAFANKGQVQDTG
                     AKMIHNAPRTSSSIVSKSIARGGGEVNYRGQVTFAKNSAKSISHIECDTIIMDDISKS
                     DTIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKEL
                     PMEYAVELNRLIAYEMEGSVG"
     gene            complement(10442..10882)
                     /gene="sufU"
                     /locus_tag="AC1PIW_000615"
     CDS             complement(10442..10882)
                     /gene="sufU"
                     /locus_tag="AC1PIW_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HIP6605574.1"
                     /translation="MALSRLDSLYMAVVSEHSKSPRHRGSLDGVEKLELHNPTCGDVI
                     ELSVKIEKDVITDIAFDGVGCTISTASASMMTEAVLGKEISQIQELAEIFSQMVRGQE
                     DPRQKELGDASLLAGVAKFPQRIKCATLPWNALKKALSLRSSAE"
     gene            complement(10869..12092)
                     /locus_tag="AC1PIW_000616"
     CDS             complement(10869..12092)
                     /locus_tag="AC1PIW_000616"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HIP6605575.1"
                     /translation="MDLERIRKEFSILDQVVNDEPLVYLDNAATTQKPQQVLDVLADY
                     YQKDNANVHRGVHTLSERATTRYEAARQKVADFIQAKSSKEILFTRGTTTSLNWVAQF
                     AKEILQPDQEVIISVQEHHSNIIPWQQACQQTGAKLRYVTLKDGELDMDHLRSLLSSK
                     TKFVSLAHVSNVLGGVVPIGEIAELVHQVGAYLVVDGAQSVPHMGVNVQELDVDFYAF
                     SGHKMLGPTGIGILYGKEELLNLMSPVEFGGEMIDFVYEQSATWKELPWKFEAGTPNI
                     AGAIGLGAAIDYLTEIGMDAIQAHEAELVDYVFPKLQAIPGLTIYGSQDLSKRTGVIA
                     FNLDDLHPHDVATALDYEGVAVRAGHHCAQPLLRYLQVPATVRASFYIYNTKADCDKL
                     VEAIIKTKEFFNGPI"
     gene            complement(12096..13358)
                     /gene="sufD"
                     /locus_tag="AC1PIW_000617"
     CDS             complement(12096..13358)
                     /gene="sufD"
                     /locus_tag="AC1PIW_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HIP6605576.1"
                     /translation="MTKEKIQEFSALQAEPTWLTDLRLKAFDKIAELDLPVIERVKFH
                     RWNLGDGSLATNDEIGAVPDFTAIGDNPMLVQVGSQTILEQLPVALVEKGVVFTDFAS
                     AMDVIPDVLEKYLGAAVAYDEHKLAAYNTAYFNATAVLYVPDNVEIDQPVEALFYQDS
                     TSPVAFNKRVLIIAGKNAKLNYLERFESLGDGAATTSANIVVEVIALAGSQIKFAAID
                     RLGKHLDTYLNRRGYLGNDATIDWAIGLLNEGNVVADLDSELKGNGSHANLKVVGLSS
                     GRQVQGVDTRVTNYGHNSVGHILQHGVILESGTLTFNGIGHIVRGAKGADAQQESRVL
                     MLSDKARSDANPILLIDENEVTAGHAASIGQVDPEDMYYLMSRGLDRATAERLVVRGF
                     LGSVITEIPVKEVRDQLIAVIEEKLTKR"
     gene            complement(13375..14148)
                     /gene="sufC"
                     /locus_tag="AC1PIW_000618"
     CDS             complement(13375..14148)
                     /gene="sufC"
                     /locus_tag="AC1PIW_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HIP6605577.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPSYEVTQGEILFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDEDKISVRDFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGINAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAQRLEKEGYVQVAAELGIDYKEDDI"
     gene            complement(14701..15873)
                     /locus_tag="AC1PIW_000619"
     CDS             complement(14701..15873)
                     /locus_tag="AC1PIW_000619"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0070085 - glycosylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIP6605578.1"
                     /translation="MIPFALKYIMVIITTMVISAIATPLVRFMSLRVGAVDNPNARRI
                     NKVPMPSAGGLAIFVAFTISVLWFLPGIVTRVNYHGTYLHYIWPLVLASSIIVLTGFI
                     DDVKELKPAPKMFGILAAATIIWIFTEFRFDSFKIPFGGPFLQFPVWLSFLLTVLWIV
                     SITNAVNLIDGLDGLVSGVSIISLTTMGIVSYFFLHDSNIFLTLTIFVLVAAIVGFLP
                     YNYNPAIIYLGDTGALFIGFMIGVLSLQGLKNSTAVAVVTPMIILGVPITDTVVAIIR
                     RKLSGQKIYEADKMHLHHRLLSLGLTHRGTVLVIYAISFLFSLTSLLLNVSSRIGGIL
                     LLVTMGLGIEILCELIGIFGINRTPLLNLLRFIGNSSYRQEKIAKYKKCCEEKKRK"
     gene            complement(15870..16592)
                     /gene="mecA"
                     /locus_tag="AC1PIW_000620"
     CDS             complement(15870..16592)
                     /gene="mecA"
                     /locus_tag="AC1PIW_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HIP6605579.1"
                     /translation="MKVKQISDSTLKITIKLDDLEERGMELSDFLIPQEKTEEFFYTV
                     LDELDLPMTFRESGMLSFRVTPKPDRVDIFVTKSDLDQNLNFDEFTDLSELGDVASMT
                     PDEFFKSLEQTVREKSAPDATAVRHLEEVEQEEEADEEEQERYIYYILEFPTIEDLFT
                     FVGTVDYPVEESELYKMDGHYYLTVLINVENRSKQYPGYILSRMLEFTNDTKLTRPAL
                     QEHGTLMLPLAAIEELRKVPTV"
     gene            complement(16720..17556)
                     /locus_tag="AC1PIW_000621"
     CDS             complement(16720..17556)
                     /locus_tag="AC1PIW_000621"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIP6605580.1"
                     /translation="MLLELLKAIFLGIIEGVTEWLPVSSTGHLILVQEFVKLNQSKNF
                     LEMFNIVIQLGAILAVMTIYFKKLNPFQPGKTKRDIQLTWQLWAKVVIACIPSILIAV
                     PLDNWFEAHFNFMVPIAIALIVYGIAFIWIENRNRGIEPQVTDLAKMSYKTALLIGCF
                     QVLSIVPGTSRSGATILGAIILGTSRSVAADFTFFLGIPTMFGYSGLKAVKYFLDGNS
                     LNMEQVWILLVASVTAYLVSLVVIRFLTDFVKKHDFTVFGYYRIILGAILLVYAFITF
                     LF"
     gene            17683..19248
                     /locus_tag="AC1PIW_000622"
     CDS             17683..19248
                     /locus_tag="AC1PIW_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HIP6605581.1"
                     /translation="MKRKLSIFLLTLIAVFSVTTGVRADEYLRVGMEAAYAPFNWTQE
                     DDSNSAVPIEGTNQFANGYDVQVAKKIAASMDKELLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTEERKKEIAFSDSYYTSIPTLVVRSDSQYANATSLEDFAGAKITAQQGVYLYD
                     LIDQIEGANKQTAMGDFSQIRQALESGIIDAYVSERPEGRTAEAANKAFKMVELADSF
                     ETNAEDVTIAVGMRKDDERIAQVNEVLATISEEEQIALMDDMIENQPVEEASEGETPS
                     FFAQVWNIVVNNWQQLLRGTGMTLLISILGTIIGTAIGLLIGVFRTAPKAANKALAIG
                     QKVLGWIINIYIEIFRGTPMIVQSMVIYYGTAQAFGLNLDRTLAAIFIVSINTGAYMS
                     EIVRGGIFAVDKGQFEAATALGFTHNQTMRKIVLPQVIRNILPATGNEFVINIKDTSV
                     LNVISVVELYFSGNTVATQTYQYFQTFTVIAIIYFILTFTVTRILRAVEKYFDTDTYT
                     TGANQMQVEVPHD"
     gene            19241..19984
                     /locus_tag="AC1PIW_000623"
     CDS             19241..19984
                     /locus_tag="AC1PIW_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIP6605582.1"
                     /translation="MTKNVILEIKNLKKSYGQNQVLKDISISVEKGEVISIIGSSGSG
                     KSTFLRSINLLETPTEGQILYHGQDVLAKGYDLTTYREKLGMVFQSFNLFNNLNVLEN
                     AIVAQTTVLKRDRAEAEKIAKENLNKVGMTEQYWQAKPSQLSGGQKQRVAIARALSVD
                     PEVILFDEPTSALDPEMVGEVLKTMQDLAKSGLTMLIVTHEMEFARDVSDRVIFMDKG
                     VIAEEGSPQQIFENPQEERTREFLQRFLG"
     gene            complement(20110..20319)
                     /locus_tag="AC1PIW_000624"
     CDS             complement(20110..20319)
                     /locus_tag="AC1PIW_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605583.1"
                     /translation="MTSYEKVAAVVGWVREKKITGYRISKETNAREMSVIALAQGRAK
                     IDNISFATALGLIDFYDKNHKKFEN"
     gene            complement(20405..21307)
                     /locus_tag="AC1PIW_000625"
     CDS             complement(20405..21307)
                     /locus_tag="AC1PIW_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIP6605584.1"
                     /translation="MVFGPIVFIGSFLFFVLIALILIASGLYVVKQQTVAIIERFGKY
                     QKTSTSGINFKIPFGVDVIAARIQLRMLQSEIVVETKTQDNVFVTMNVATQYRVNENN
                     VTDAYYKLMHPEAQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQKQVAEEMSTYG
                     YVIVKTLITKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRL
                     HGVGIAQQRKAIVDGLADSIRELKESNVSLSEEQIMSILLTNQYLDTLNNFAQGGNQT
                     IFLPGNPEGVEDIRTQILSSLRAK"
     gene            complement(21317..22567)
                     /gene="ilvA"
                     /locus_tag="AC1PIW_000626"
     CDS             complement(21317..22567)
                     /gene="ilvA"
                     /locus_tag="AC1PIW_000626"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019322143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HIP6605585.1"
                     /translation="MITAKNVEQAYSVLKRVVVRTPLDYSRYLSEKYGATIYLKRENE
                     QRVRSFKIRGAYYAISQLTDDEKSRGVVCASAGNHAQGVAYTCQEMQIPATIFMPITT
                     PQQKIGQVKFFGGDYVEIRLVGDTFDESAKAAQDYTQESGKTFIDPFDDENVQAGQGT
                     VAYEILEEAEEQTISFDQILVPIGGGGLVAGVSAYLKEHAPEIKIVGVEASGARSMKA
                     AFDKGRPVKLDQIDKFADGIAVQKVGKSTYEVARKYVDRLIGVDEGWISGTILDLYSK
                     LGIVAEPAGAATIAALEVVKDQIKGQTICCIISGGNNDINRMPEMEERALIYEGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRANKGTGPVLIGIALGDKTDYTQFI
                     SRLEEFDPQYINLHENDSLYKMLV"
BASE COUNT         6648 a         5138 c         4327 g         6469 t
ORIGIN      
        1 attgttcgag ctaaaaaccg cctgcacaag gtcttacagg tcactttccc tgaattggaa
       61 aatatcttgt caacaccatc tggcgaacaa tactggaact tagttatagc ttttccttgc
      121 aaggacttcg tgcttgagtt aagcaatgat gaactctcaa agagcatccg tctgtccact
      181 tcaaaacgga tttctgataa gcgtgtggct tacttagcag aaaagcttat agcactagct
      241 aatcaatctt attgtgccgt caagaaaacc tctccaatac tggaagaggt ccgttactat
      301 gcaaagactt tatgaacaga gacaagctgt cttagacgaa atggtggaac tagctcagcc
      361 attacctgaa tatgacattc tgctctctat tcctggaata gctgagacta ctgcaacaag
      421 tattattggt gaactgggag atattcgccg ttttcagtca gccaaccaaa tcaatgcctt
      481 tatcggtatt gacctgagac actatgaatc tggtaacttc ctcgctaagg aacacattac
      541 caagcgtggc aatccctacg ctagaaagat tctgttcaaa tgcattcaca atatcgcttc
      601 agccagtcat accaatcctt gccatatcgc agacttttat gagaaacgaa aaagacaatc
      661 gcaaacgact tcaacgaagt cgcacacgat tgcctccata catcgtctca ttcggacaat
      721 gtattacctc attatgcata acaaacttta cgattacgct tcaactcaaa atcggtaaaa
      781 ctgtttatgc catatcattg taacacctta tcaaaaaatt tcaacataag gtgttggttt
      841 tgtatgcact ttttacacta aaatttagtc caaaataaaa caatttcctt attttgacta
      901 ttgaactcaa aatatttttc gtcaaatacc ttgatggact tgacaaatag tagaaaaaag
      961 agttcccaaa ggaacccttt tcacaattaa cctaaacgct gcttagcatc tgctgcacgt
     1021 tggaaagctt gtccgacata gttgatacag agcatcatca caaggatgaa gatggctgct
     1081 ggcaaccaaa tccatgtctt gttttccaaa atatctgcat tgcgagcatt ggcaatcaag
     1141 gtacccaaac ttggtactgt cgatggcaga ccgaaaccaa gataagtcaa cgaagcctcg
     1201 ataccgatat ttcccgcaaa gttcaaggtc aagttaacga taatcaatga acttaagttt
     1261 ggtaaaattt cacggaacat aatcataaag tcgcttgttc ccaatgtttt tgatgcattt
     1321 acatagtcca aacttgcctc tgacaaagtc cttgtacgga atagacgagc cttggccgtc
     1381 caataaaagg cactcatgat gagaatgaaa tgatagattg tatagttctt gataactgtc
     1441 acgaaaacaa taatcaacat tgtcaccggc aaaatcatga tgaaatcgac aatacgcatc
     1501 aagaatccat ctaaacgacc gccataataa ccagaaatca gaccgacaga aatcccgaca
     1561 atacttgtaa taatcgtaat ggtaaaaccg ataatgattg agttacgagc gccgataatc
     1621 agctgaccaa agatatcttt accaccttca tctgtcccca agatatagcc gtcaacacct
     1681 ggttccaagt aacgtcccaa aaggttgact ttcataactt gttcttgatt caacagaagt
     1741 gcaccgataa agactgctaa gagaaccgtc actaagataa ttaatgaggt caatgcaagt
     1801 cgatctttca taaactcccg cgcgatgaca cggaagccac ctggaggagt tgaagcagat
     1861 tgtacttgtt ttttattttc tttactcacc ttactcctcc ttctatttca cacggatacg
     1921 tggatccacg atactcatga taatatctga aatcaatgtc cccaacaaag tacccatacc
     1981 aaataccaac aataaagcta agataacact atagtcacga ctagaaatag agttaaagaa
     2041 taactgaccg atacctgggt aagtgaaaat attttcaata aagatagaac caccaattaa
     2101 tcctgtcaat tcatagccca agaaagatgc gattggtaaa atcgaattac ggaaaatatg
     2161 acggttataa acaactctct caggaacacc tttggcacga gctgtacgaa catagtcctg
     2221 actcttagca tcaataatcc ccgtacggag atattgaata gtaactgttg ttgacaaaat
     2281 agccattgta atcgctggca gaatcatatg gtgtaaacga ctgagagtcg ctgcaaatcc
     2341 ttccacacct ccactaatcg aaccacgcgt cgggaaccag cccaaactaa agccaaacat
     2401 ccagagcatg agaatggcaa aaacgaaagt tggagttgaa aatgtcaaga agttatacac
     2461 gccaatgatt ttatctgcaa gtgagttttg ataacgacct gcaatcatac ccaatggaag
     2521 tgcaatcaaa taagtcaata ccatcgtcaa gagggataat ccaattgtat tattcaagcg
     2581 ctgcatgatg acatcaacaa ctggttgttt aaaaataaca ctctgaccaa aatctccttg
     2641 caaaagattg cctacccagc ggaaatactg aacagggatt gggtcatagt aacctgccgc
     2701 aatccgtttt tgttcaatga ttgctggatc aatattgggg tcaatcaatc ctgtaaaggg
     2761 atcccccggc atcatcttcg caacaaagaa cgcaatgaca ctcaaaataa taatctgcgg
     2821 aatcatcatg agtaaacgac gtaatactgt tttccacatc ttagttctga cctccttctg
     2881 gaagggctac ttgatgagtt tctgagaagg atctcaaatc gtacacacga ccgttttcat
     2941 catagtattg accttgcatt tcttgatatt ctttttcagc agccaaacgc ttttctttat
     3001 tcttcaatcg attgagtggg tcaatcgttg gaatagctga caacaaacgt ttagtataaa
     3061 tgtgttgtgg attattataa atatcgttct tatttcctgt ttctacgaaa cgtccgcgat
     3121 acatgatgaa caattcatca cacatgtgtt gcacaacacc gaggtcatgg gaaataaaga
     3181 gatagctgag cttaaactca tcttggatac gtttcatata attcaagacc tgtgcctgca
     3241 cagacaaatc caaggcagaa accggctcat ctgcaataat caaacgagga ttactagcca
     3301 aagcacgagc aacaccgata cgttggcgtt gaccacctga gaattcgtga ggatacttaa
     3361 tcagagcttc ttcatttaga ccgattgtat ccaagagttg aagaactttt ttcttttctt
     3421 catctggaga taaacggtca aagttacgaa ttggttctgc aatgatgtcc aagatacgtt
     3481 ttttcgggtt gaaacttgaa agagaatctt ggaaaatcat ttgaacatca cgattaaagt
     3541 ttcctttttt accacgtgat ttgttggtta catcttgtcc ttcatagata atctgaccag
     3601 aagtggcacg ctctaaaccg ataattgcct taccaatggt tgatttccca gaacctgact
     3661 cacctaccaa accataggtt tttccttctt caaactcaat attaacacca tccacagcat
     3721 agacatggtc cgtcacacgg ttaaagaagc cactacgaat tggataatgg acttttaaat
     3781 cttttacttc aataaatccc actattaggc ctccccttca aaatagaatg tctcatggca
     3841 ggtacaacga acaaagtgat ttggacctac ctcgtgcatg ctcggattct cttcatgagc
     3901 ctctgctcca atccacggaa tacgcggagc aaaacggcaa cctttacgaa ccatttttgt
     3961 aatcggcggc acaatacctt caataacatg aagatcgcct ccttcgctac ctgattgagg
     4021 atttgacttc aagagcgaac gtgtatatgg atgtttagga ttggtaaaca attcttctac
     4081 tggcgctact tcgacaaact gaccaccgta catgaccgcc acacgatcag cagtttctgc
     4141 aactactccc aagtcatggg taatcaaaat aataccagaa cctgtctccg cttgaatatc
     4201 atttaagaga tccaagattt gtgcttgaat tgtaacatcc aaggctgttg taggttcgtc
     4261 cgcaataata attggcggtt tacaagacaa ggccatcgca ataatgatac gctgacgcat
     4321 accacctgaa agctcatgtg gatactgttt aaatgtccgc ttaggatttg gaatacctac
     4381 ctgagccaag agttccaaaa ctcgctctgt acgagcattg gcatccaaat cagtatgata
     4441 gaacagtgcc tcatcaatct gtgcaccaat cgtcatcaat ggatttagag aagctaaggg
     4501 atcttggaaa atcataccaa tatcattacc acgtactgta ttgtacttag cttcatccat
     4561 gccaatcaat tccatatcat tgtactggat gcttcctgtg attttggtat tcaaagggtt
     4621 atgcaagccc atgatggtcg ttgccaaagt tgattttcca cagccagatt cccctacaat
     4681 tgccaaaatt tcattttttt gcaatgagat gtcaacaccg tcaacagcgt caaaataatc
     4741 atctccaata cggaaaccga cgtgtaaatc tttaatgtct aaaagcggtt tttcggtagc
     4801 cacgcttttt cctcctttat gtgccagcta atcaagctca atcaattcaa tagccttacg
     4861 attatcaagt tcatataacg tttgattaac tgactttata taactactct agaataacat
     4921 atttctacca aattatcgtt tgatagcgat tacccagaag taaaaaatgt acctatatcc
     4981 aatcataaat caagaccatc cctatcactg tctcaattag gaattgagta ggttaatgtt
     5041 cttcatcttg agtttttgtt agtagggact catttttgtg tactgagtct actataccac
     5101 aattatctga aaattccaat agcaatctga aaaaagccaa tcaaattggc tttttcagat
     5161 tctattgtct tctaacaaat tattctgcca ctacaccctt atcagctgtc aattcgatac
     5221 cctcataagc aagacctgta cccgaagctg agccgtattt tgtatcatag cttttcacac
     5281 gtttattaac agcagtaatt ttttctgact caaacgttgg aatagcaaat gcttgctcgt
     5341 gagcatattt ttgccaagcc ttatagttct caacgttttt cttatcatca aacgactctg
     5401 ctgagctgat agcttccaat aacttggtgt tttcatcgga cacgaaacgt gacatattga
     5461 aggctgcgat aggcccccat agaccacttg ggtttggatc gtaacctgtt gaccatccac
     5521 cagcatacat atcaatagca gcatcattag cctgaactga gttatagaac gacttacctt
     5581 caactgtacg tcctgtgtaa agttcgacat tcaagccaac ttctttccac caagcaatgt
     5641 attgttgtac aagcgcttcg ttagcttcag tacgtttacg agccgcaaag gtaatcttga
     5701 atgccttacc atctttgcct tcacggatac catcaccatc tacatctttg tagccagctt
     5761 catccaaaag ctttttcgct ttttcaggat tgtatgaata gccttccaac tctgaatcat
     5821 gaatatcgcc gaagaatgag ataatcaatg attttgcagg gtggtaaaga ccgttgtata
     5881 atgacttacc agctgtcttg gtatcaatcg cataggcaat cgcttgacga agtttcacat
     5941 cattcatctt ggcattttca tccataacgt tcttaccagc tgcttcgtca tatttaccaa
     6001 aattaaacga gatatactca taagacgact ctaactgacc aacgatattc aagttagaag
     6061 catccttata tgaatctaac tgatcaaccg gcatttctgc gatatcataa ttccccgctt
     6121 tcatttcaga aacaatagta tcaggagata cgatgtcaat cttcaatgag cttgtttttg
     6181 gtttcgtacc cttgaagaag tattcgtttg gtacgtaagt gatagactca ccattgacga
     6241 tttccttaat cttccacggc cccataccaa caagcttcgc agtacgtgag tattcgctct
     6301 tttcccagtc tgcaacaggg atgtctttat agatgtgttc tggttggata taggctggga
     6361 catcaccacc tgcatacatc attgaaggtg acatttcttt tacagttaat tctactgtat
     6421 agtcatcaac tttcttaata ccagagatgt ctgatgcttt tccatcaaca aattcttgca
     6481 taccttcaat attcaagaat ttatcatcaa aacgaatacc tgtatagtct ttgcttgcca
     6541 tcgacttaat tgtaaagatg tagtcgttga ttgtgaatga ctcaccatct gaccacttgt
     6601 aatccttacc agtcaagctg actgtgattt tcttgccttt tacatcaaat tcagccttgg
     6661 caagacctga gtcgtccaac ttacgttctc cgtcgtaacc gaacattgaa atatcaacca
     6721 taccaccaaa tgttgaatca gtagtattct cagtcaactc atcaattaaa agacctgatg
     6781 aagttgtagg agatacccaa gcatatttca attgagcatc tgcgactgct gttccatctt
     6841 gttttacttc agccgggaat gacaattcct gttttgctgc acctgattgt tttgaaccac
     6901 atgctgcaag aacagatgct gaaaggagag tcacacctgc aagtgcaaaa agtttcgttg
     6961 tctttttcat aaaacacctc tgttttcttt ttgtttgaca cttattacta tgtcttataa
     7021 aaatcattat atcataaatt tttcttaaat aaagtctttt tgtcagaaaa ttttgtaaaa
     7081 acatattttt tcatcatttt ctcatttttt acatgttttt tccaaatttt tctcacattt
     7141 cttgtgatat aatagaataa aataccccaa aaaggaatga ttatgcgtaa acttttacta
     7201 acactcacta caatccttgc tgttttttta acttccacac ctattttggc agaagatttt
     7261 tctgttgcag ccaagcatgc tatcgcggtt gaagtaagta gcggcaaaat cttgtatgag
     7321 caagatgccg aaaccaaagc aagcattgct tctatcagta agacattgag catttacctg
     7381 gtctacgaag cactggcaaa gggggaaatc actttagata ctatggtaga tatttctgat
     7441 tatccctata gcctcacttc taatactgaa ttaagcaatg tcaatctaga tgaacgctcc
     7501 tattctgtaa gagacctatt aaacgcttct ttaataacat catccaacag tgctattatt
     7561 gctctggcag aaaaaatagc cggtagcgaa gctgcattcg tggatcgaat gaaggcaaaa
     7621 gtccaagaat ggggaattac cgatacaaaa attgtcaatg tatccgggct agataatacc
     7681 gacttgggtg aaaatattta tccaggttcc agtcctgaag atgtaaatat gttttcagcc
     7741 ctagatgttg ctatcatcgc aagacgatta atactcgact atcctcaggt actcgacatt
     7801 acctcgctaa acgcctacga ttttggcggc tacacttact atagtaccaa ccaaatgctg
     7861 agcgacggca ctcacgctcg tggaggggtg gacggcttaa aaacagggac ctcaaactcg
     7921 gctggttcaa gttttcttgc aacaactcaa caagctggta tgcgcattat cacagtagta
     7981 ttgaatgcca atgatgggct gacctctcca gaaaatcgct ttgtcgcgac aaatgatttg
     8041 atgaactatg tttattctaa cttttcgctt gttacactag taaaaaaaag tgaagccttt
     8101 ggagatagta agataaaggt tttcaacggt gaaaaagaaa caagtcctgt tgttgcagca
     8161 gcagatttaa ctgttgttca acgaaatcaa actgataatg tccccaccgc aactttcaca
     8221 acggatatca aagaaattga tgctcccctc aaagcaggga ccttggtcag caaactacaa
     8281 ctgaaagatc aagatctcat tggaaaaggt tatatttccg agcaaactgg tgtcgatatg
     8341 attatcccta gcgatatgaa agaagcatcc tggccattct cttggtggaa tcaatttgtc
     8401 cgctatgtca atgaaaaatt ataaaaaaat agtatgtcga acgattttcg acatactatt
     8461 tttttattgt agagtgacaa ccgctactcc ttatctccag cctcggttgg agagtgagtt
     8521 ggtgagagtt cctgacagaa aatgacgagc gtggaaggta acaattccat tcgtcaattt
     8581 ataaagaaga gctactgccg tcgctcctca tctcctactt cagtggggag tttgacgggc
     8641 ttgaacagct ttaaagctgt tcaagttcgc ggcaaactat cccactgaac cctccatctc
     8701 ataggcaatc agtctgttga gttcgactgc gtattccatt gggagttctt ttgtgaatgg
     8761 ttcaacaaag cccatgacaa tcatctctgt agcttcggac tcggacaagc cacggctcat
     8821 gaggtagtag agctgttctt ctgagatttt tgatactttg gcttcgtgtt cgagggccac
     8881 ttgtgagttg tggatttcat taaatggaat ggtatctgat ttggaaatat catccataat
     8941 aatggtgtca cactcaatgt ggctgataga cttggctgag tttttcgcaa aagtgacttg
     9001 accacggtag ttgacttctc caccaccacg ggcaatggat ttggacacga tagaagatga
     9061 ggtgcgtggg gcgttgtgaa tcatcttagc accggtatcc tgtacctgac ccttgttggc
     9121 aaaggcaatg gacaacatgg ttccacgtgc cccttcacct tctaggtaaa ccgctgggta
     9181 tttcatggtc gtcttggcac cgaggttgcc gtcaatccac tcaactgtgg catttttctc
     9241 ggcacgggca cgcttggtta ccaagttata gacattgtct gaccagttct gaatggtggt
     9301 ataacgcata taggcacctt cgtgggcgat gatttccaca atcgctgcat gaagacttgc
     9361 cgttgagtag gtcggtgccg tacagccttc tacgtagtga acacttgccc cttcatctac
     9421 gatgatgagg gtgcgttcaa actgacctgt tccttcattg ttgatacgga agtaggtttg
     9481 gagtggaata tccagcttga caccttttgg tacatagatg aaggttccac ctgaccaaac
     9541 cgctgagttg agggcagcta gtttattatc tgacggcgga acaagttttc caaagtattt
     9601 tttgaagagt tctggatgtt ccttgagagc cgtgtcggta tccgtaaaga taatgccatg
     9661 cttgtcgtac tcttccttca tgttgtggta gaccacttct gattcatact gggcagaagc
     9721 acctgctagg taggcacgct cggcctctgg aataccgatg cgttcaaagg tttccttgat
     9781 tttgtctggt acatcatccc agctgcgtgc tggcttatct gacggttttt ggtaatagac
     9841 aatgtcatca aaatccagct cagacaagtc ggctccccaa gtctgcatcg gcattttctt
     9901 gaaggtttcg taggatttca aacggaattc caacatccat tcaggctcgc ctttgatacg
     9961 ggacatttca cgaatgactt cttcgttcaa acccttacct gtcgaagcca ccagttccac
    10021 gttctcgtca tggaaaccga atttgtattc tccaagatca atcggggttg gttcaattct
    10081 ttcttctgac ataatataag atactaagcc ccttaagaaa gcaaatgaaa aacaggaaat
    10141 cgacttcgtg tgcaaacaca cgcggagatt tatctttttt caaaagcttt tagggcgagt
    10201 tcaatttcat ttccaatcag aaactgaaca atcctttctg tatgattttc atttttgttt
    10261 attcgatgat gctcagtaag catcttcact tcgttcgatt gctttcttca agacactaaa
    10321 cttggttgga actaaagttc ctaaccaagt aacgcaaggt tctaatcaac cttgctaagt
    10381 ggcttactaa ttggctgatt ttggtaatat cgaccaaaat cgctcatgtc gcagttaccc
    10441 ttcattcagc actggaacga agtgacagtg ctttcttcag ggcattccac ggtaatgtgg
    10501 cacatttgat gcgttggggg aatttggcaa cacctgcgag gagggaggcg tctcccagtt
    10561 ccttctggcg tgggtcttct tgacctcgga ccatttgtga gaagatttct gctagttctt
    10621 gaatctgact gatttctttg ccaagcactg cttcagtcat catagaagct gatgcggttg
    10681 aaatggtgca gccaacgccg tcaaaagcaa tatcggtaat cacatctttt tcaatcttga
    10741 cggacaattc aatcacatcg ccacaggttg ggttgtggag ttctaacttt tccactccgt
    10801 ccaagcttcc gcgatgacga ggcgacttgg agtgttcaga cacaactgcc atataaagag
    10861 agtctaatct agatagggcc attgaaaaac tcctttgtct tgatgattgc ctcgaccaac
    10921 ttatcacagt cagccttggt attgtaaatg taaaaacttg ctcgtacagt agcaggcacc
    10981 tgcaaatatc tgagaagcgg ttgggcacaa tggtggcctg cccgcacagc aacaccttca
    11041 tagtccagag cagttgccac atcgtgtgga tgcaagtcgt ccaagttaaa ggcaataact
    11101 cccgtccgct tggacagatc ctgactgcca taaatggtca agcctggaat agcctgcaat
    11161 tttggaaaga catagtcaac cagttctgcc tcgtgggcct ggatagcgtc cataccaatt
    11221 tcggtcaggt aatcaatggc cgctcccaga ccaatcgcac ctgcaatatt gggcgttcct
    11281 gcctcaaact tccaaggcaa ttccttccaa gtagctgact gctcatagac aaagtcaatc
    11341 atttcaccgc cgaattcaac tggcgacatg agattgagca attcttcctt gccgtagaga
    11401 atgccaattc ctgtcggtcc caacatttta tgacctgaaa aggcatagaa atccacgtct
    11461 aattcttgca cgttgacgcc catgtgggga actgactggg ctccgtccac caccaagtag
    11521 gcaccgactt gatgcaccag ctctgctatt tccccaatag gaaccacgcc acctaggaca
    11581 ttggatacgt gggccaggga gacgaacttg gtcttagaag atagtagcga ccgcagatgg
    11641 tccatgtcta gctcgccgtc ttttagggtc acatagcgga gcttagcacc cgtttgctga
    11701 caggcttgct gccaagggat gatattggag tggtgctctt ggaccgagat gatcacttcc
    11761 tggtctggct ggagaatttc ctttgcaaac tgagccaccc agttaagact agtggtcgtt
    11821 cctctggtaa agagaatttc cttgctagac ttggcctgaa taaaatctgc caccttctgc
    11881 ctagccgcct cgtagcgagt agttgcccgt tccgaaaggg tgtgaacgcc acggtggaca
    11941 ttggcattgt ccttctggta ataatccgcc agcacatcta gtacctgctg cggtttttga
    12001 gtggtcgccg cattgtccaa atagaccagc ggctcatcgt taacaacttg gtctaggatt
    12061 gaaaattcct tgcgaatgcg ttccaaatcc atatcttatc tctttgttag tttttcttca
    12121 atcacggcaa tcagttggtc acgaacttcc ttaacaggaa tttctgtgat aacagagcct
    12181 aggaaaccac gaacaaccaa gcgttcagct gttgctcggt ccagaccacg actcatgagg
    12241 tagtacatgt cttctggatc cacctgaccg attgaggctg cgtgtccagc tgtgacctca
    12301 ttctcatcga taaggagaat tgggttagca tctgagcgag ccttatcaga caacatgagg
    12361 acacggcttt cctgctgggc atccgctcct ttagcaccgc ggacaatgtg tccgatgcca
    12421 ttgaaggtca aggttccact ttctaagata accccatgtt gcaggatgtg accgaccgaa
    12481 ttatgaccgt agttggtcac gcgcgtatcc acaccttgga cctgacgacc tgatgacagt
    12541 ccgacaactt tgaggttggc atggctaccg tttcctttta attcgctgtc caaatcggca
    12601 accacatttc cttcgttgag cagaccaatc gcccagtcaa tcgttgcatc gtttccgaga
    12661 tagccgcgac ggttgaggta ggtatccaaa tgcttgccaa ggcggtcaat ggccgcaaac
    12721 ttgatttggc taccagcaag ggcgattact tccacgacaa tattggctga agtcgttgct
    12781 gcgccgtcgc ccaaactttc aaagcgttct agataattaa gcttggcatt ttttccagcg
    12841 atgatgagga cacgtttatt aaaagcaact ggactagtgc tgtcttggta gaaaagggcc
    12901 tcaactggct ggtcgatttc aacattatct ggcacgtaga ggacagctgt tgcgttaaaa
    12961 taggctgtgt tataggcggc taatttgtgt tcatcgtagg ctacggcagc gccgagatac
    13021 ttttcaagta catctggaat gacatccata gccgatgcga agtcagtaaa gactacacct
    13081 ttctctacaa gggcaaccgg caactgttct agaatcgttt ggctgccaac ttgaaccaac
    13141 ataggattgt caccgatagc tgtaaaatct ggaactgccc cgatttcatc atttgtagct
    13201 aggcttccgt cacctagatt ccaacggtga aacttgaccc gctcgataac cggcaagtcc
    13261 agctctgcta ttttgtcaaa agccttaaga cgcaggtctg tcaaccaagt tggttctgcc
    13321 tgcaaggctg aaaattcttg aatcttttct ttggtcatag cttcctccaa agacttaaat
    13381 gtcatcttct ttgtagtcga tgccaagctc tgctgcgact tggacataac cttctttttc
    13441 caagcgttgt gccaattctg gtccgcctga cagaacaaca cgaccttcca tcatgacatg
    13501 gacaacatca ggagtgatgt agttgagcaa gcgttggtag tgagtaataa tcatggcacc
    13561 aaagccttca ccacgcatag catttatccc ctttgacacg actttaaggg catcgatgtc
    13621 caaacctgag tcaatctcat caagaagggc aaaagttggt tccaacatga gtaattgcag
    13681 aatctcattg cgttttttct caccacctga gaaaccttcg ttgaggtagc gctctgccat
    13741 ttcctctttc atgttaagga gctccatctt ttcatccaac ttagtaatga aatcacgaac
    13801 agaaatcttg tcttcgtctt ccttaccagc gttcattgcg gcacgaagaa attccgcatt
    13861 agtaatccct ggaatttctg acgggtattg catagccaag aaaagaccca tacgggcacg
    13921 ttcatcgact tctaattcta aaatattgac accgtcaaac agaatctcac cttgggtaac
    13981 ttcataactt ggattcccca tgatggctgc tgaaagggtg gatttccctg ttccgttcgg
    14041 tcccatgatg gctgcaattt cacctgtttt aagggtcaaa ttcacaccct tcaagatttc
    14101 tttgccttca atttccacat gaaggtcttt gatttctaat actgacatgc tgtgctcctt
    14161 ttcatttttg tcctcttcaa aaaccacatt aactgaccaa gctaactaga tacaccgata
    14221 agactcaact ttggcagtgg ggaaagagct cgaccattca tagaagagtc cgtcaacaag
    14281 tcctagtttt aagttgttga gctgggagtg ggaaagaact cgaccatcca tagaagagtt
    14341 cgttttccca cccccgcaca gttgattagg tcagatttgg agtgtaaaac acgaacaaat
    14401 ctgccaatca accactactg cgctaagatg ttgacactaa cgttgagaag cggtacaggg
    14461 cttgagccca gcttcactcc actggactgt taaacccaat caaccactac tgcgctgaga
    14521 tgttgacgct aactttgaga agtgatgcgg ggcttgagcc cagcctcttt tcttgttaat
    14581 gatgattttc agagagtgtt aatctataaa atagtctttt ctgacaattt taattaacca
    14641 tatccactat attatatcaa aaaaagagcc tgacggctct tatcttgttt agaaagattc
    14701 ttattttctt tttttctctt cacagcactt tttatacttt gcgatttttt cttgacggta
    14761 actactatta ccaataaacc gaagaagatt gagcaatgga gttctattaa taccaaagat
    14821 gccaatcaat tcacagagaa tctcaatccc cagtcccatg gtcaccaata ggagaatccc
    14881 ccctattcta ctcgaaacat tgagcaaaag agaggtcagg gagaataaga aagagatggc
    14941 gtaaatcacc aagactgtgc cacgatgagt aagtcccaga gagagcaagc gatgatgcag
    15001 atgcatctta tctgcttcat agattttttg cccagacaat tttcgacgaa tgatggctac
    15061 tacagtatcc gtaataggca ctccaagaat aatcatcgga gtaaccactg caacagctgt
    15121 cgaatttttc agtccttgaa gggacaagac tccaatcata aacccgatga agagggcacc
    15181 tgtatcgcca agataaatga tagcaggatt atagttatac ggtaaaaaac ctacaatcgc
    15241 cgcaaccaaa acaaagatgg ttaaggtcaa aaaaatattg ctatcatgca aaaagaaata
    15301 agacacaatt cccatcgttg tcagggaaat aatcgatact cccgaaacca gaccatccag
    15361 accatcaatc aagttgactg cattcgtaat ggaaacaatc caaaggaccg ttaatagaaa
    15421 agagagccag acaggaaatt gaaggaaagg tcctccaaaa ggaatcttaa agctatcaaa
    15481 acgaaattcc gtaaaaatcc aaataatcgt cgctgcagct agaataccaa acattttagg
    15541 agcaggcttt aactccttga catcatcaat gaatcccgta agaacgataa tgctggatgc
    15601 taggaccagg ggccagatat aatgcaagta agtgccatgg taattgacac gggtaacaat
    15661 tcctggtaaa aaccatagga cagaaatggt aaatgccaca aagatagcca gacctcccgc
    15721 tgaaggcatg ggcaccttat ttattcgccg tgcattggga ttatcaactg ctccaaccct
    15781 cagggacata aaacgaacca agggggtcgc aatggctgat atgaccatgg ttgtaataat
    15841 gaccattatg tattttagag caaaaggaat catactgtcg gaaccttcct aagctcctca
    15901 attgccgcaa gcggaagcat caaagttccg tgttcttgta aagcaggtct ggtcaactta
    15961 gtatcattgg taaattccaa cattcttgag agaatgtagc ctggatactg cttggaacga
    16021 ttttctacat tgataagaac tgtcaagtag tagtgaccat ccattttata taattccgat
    16081 tcttctactg gataatcaac cgtaccaaca aaagtaaaca gatcctctat cgttggaaat
    16141 tccaaaatat agtaaatata gcgctcctgc tcctcttcat ctgcttcttc ctcttgctct
    16201 acctcttcca aatgacgaac agctgttgca tccggtgcac ttttctcacg aacggtttgc
    16261 tccaaactct tgaaaaactc atctggagtc atacttgcga catcaccaag ttccgacaaa
    16321 tctgtaaatt catcaaaatt caagttctga tccaaatcag atttggtaac aaagatgtcc
    16381 acccgatctg gcttcggagt cacacgaaaa ctcagcatac cgctttctcg gaaagtcatt
    16441 ggtaaatcca attcatccaa aacagtgtaa aagaactcct ctgtcttttc ttgcggaatg
    16501 agaaagtccg aaagttccat tcctctctct tccaaatcat ctaattttat agtgattttc
    16561 aaggttgaat cactaatttg ctttactttc ataactacct cattcattga ttcatcaatt
    16621 ttttaaaatt gattgatcat tttcaatact catacatatc ttccaccatt atactaaaac
    16681 caagctcgaa agacaaaaga aaagcaggtc tccctgctct taaaataaaa atgtgataaa
    16741 ggcataaacc aataaaatcg cccctaaaat gatgcggtag taaccaaaga ccgtgaaatc
    16801 atgtttttta acaaagtctg tcaagaaacg aataacaact aaggatacca agtaagccgt
    16861 cacactagcg actagcaaga tccatacctg ctccatattc aggctattgc catctaagaa
    16921 atatttaaca gccttcaaac cactataacc aaacatggtt ggaatcccaa ggaagaaggt
    16981 aaaatctgcc gccacagatc ggctggtccc caaaataatc gctcccagaa tggtcgcacc
    17041 tgagcgacta gtccccggaa cgatgcttaa gacctggaaa cacccaatca acaaggcagt
    17101 tttataagac attttcgcta agtccgtcac ctgtggctct atacctctgt tacggttctc
    17161 aatccaaata aaggcaatac cgtagacaat caaagcaatc gcaatcggta ccataaaatt
    17221 gaaatgtgct tcaaaccaat tatccaacgg gactgcaatt aggatggacg gaatacaggc
    17281 aatgacaacc ttggcccaca attgccaagt caattgaata tcacgcttgg tttttccagg
    17341 ctgaaatgga ttcaattttt taaagtaaat agtcataact gctagaattg ctcctagctg
    17401 aatgacaata ttaaacattt ctaggaagtt cttactttgg tttaatttca cgaactcttg
    17461 aaccaaaatc aaatgccccg ttgaagaaac tggcaaccat tccgttaccc cttcaatgat
    17521 tcctaaaaat atagccttca acaattccaa tagcataaaa agctcctttt catcaattct
    17581 ctacattata tcataaaaaa actccctatt catagtacaa ggatattgaa atttgtcaga
    17641 atttttaata aaatggtgta atagcatttt aaaggagaca ctatgaaacg taaactcagt
    17701 atatttctgc tgaccctaat cgctgttttt tctgtaacaa caggggttcg ggcagacgaa
    17761 tacctgcggg tcggtatgga agctgcctat gcaccattta actggaccca agaagatgat
    17821 agcaacagtg ccgttcctat tgagggaacc aatcaatttg ccaacggata tgatgttcag
    17881 gttgcaaaaa aaatcgctgc atccatggat aaagaactct tggttgttaa aaccaagtgg
    17941 gaaggcttgg tccctgctct tacatctggt aaaatcgata tgattatcgc cggtatgagc
    18001 ccgacagaag agcgcaagaa agaaatcgcc ttctcagata gctactacac ttcaattcct
    18061 accctggttg ttcgttctga tagtcagtac gcaaacgcaa ccagcttgga agattttgct
    18121 ggcgcaaaaa tcactgccca gcaaggggtt tacctctatg atttgattga ccaaattgaa
    18181 ggggctaaca aacagactgc catgggagat ttctcacaaa tccgtcaagc tttagaatct
    18241 ggcattatag acgcctatgt ttctgaacgt ccagaaggtc gtacagcaga agctgcaaat
    18301 aaagctttca aaatggttga attggcagac agttttgaaa ccaatgccga agacgtgacc
    18361 attgctgtcg gtatgcgcaa agatgacgag cgtatcgctc aggtcaacga agtcttagca
    18421 actatttcag aagaagagca aatcgcactt atggatgata tgattgaaaa ccagcccgtt
    18481 gaagaagcca gcgagggcga aacacctagt ttcttcgccc aagtttggaa tattgttgtt
    18541 aacaactggc aacaacttct gcgtggaact gggatgacat tactcatttc catcctcggt
    18601 acaattatcg gtactgctat cggtctcctc atcggtgtct tccgtacagc tccaaaagca
    18661 gcaaacaagg cgcttgctat tggtcaaaaa gtgctcggtt ggattatcaa tatctacatc
    18721 gaaattttcc gtggtacgcc gatgattgta caatccatgg ttatttacta tggtaccgcc
    18781 caagcattcg gactcaatct tgaccgcaca cttgccgcta tcttcattgt atccatcaac
    18841 acaggtgcct acatgagtga gattgtccgc ggtggtatct ttgccgtcga taagggccaa
    18901 tttgaagctg ctactgctct cggatttacc cataatcaga ccatgcgtaa gattgtcctt
    18961 ccacaggtta ttcgtaacat cttaccagcg actggtaatg aatttgtcat caatatcaaa
    19021 gatacttctg tattgaacgt tatctcagtt gttgagctct atttctcagg aaatactgtc
    19081 gcaacccaaa cctatcaata tttccaaacc tttacggtta ttgctattat ctacttcatc
    19141 ctaaccttta ctgtgacacg tatcttacgc gcagttgaaa agtattttga tacagataca
    19201 tacacaacag gtgcaaacca aatgcaagtc gaggtgccac atgactaaaa atgtaattct
    19261 tgaaatcaaa aatctaaaga aatcatacgg acaaaatcaa gtcctcaaag acatctctat
    19321 tagcgttgaa aagggcgagg ttatctcaat catcggttca tctggttcgg ggaaatcaac
    19381 cttcctccga tctatcaacc ttttggaaac accaacagaa ggacagattc tataccacgg
    19441 acaagatgtt ttggctaaag gatatgattt gacgacttac cgtgaaaaac taggtatggt
    19501 attccaatcc ttcaaccttt tcaacaatct aaatgtattg gaaaatgcca ttgtagcaca
    19561 aactacggta ctgaaacgag atcgagctga ggcagagaaa atcgccaaag aaaatctcaa
    19621 caaggttggc atgaccgaac agtactggca agccaaacca agtcaactct ctggtggaca
    19681 gaaacaacgc gtggccattg cacgtgccct atctgttgac ccagaagtta tcctctttga
    19741 tgagccgact tctgctctcg atccagagat ggtcggggaa gttctcaaaa ccatgcaaga
    19801 tttggcaaaa tcaggattga cgatgcttat cgtaactcac gaaatggaat ttgcccgtga
    19861 cgtatctgac cgcgttatct ttatggataa gggtgtcatc gccgaagaag gcagccctca
    19921 acaaatcttt gaaaatccac aggaagaacg cacacgtgaa ttccttcagc gttttcttgg
    19981 ataaactcaa tctattccat agaagactat tacttaccag tttcaaagct ggtaagtttt
    20041 tttagactaa aaaaaagagg aattcgtaaa attcctcttt ttggacatct ctaaatgaaa
    20101 ccatttagat tagttttcaa atttcttgtg gtttttgtcg taaaaatcaa tcaaacccaa
    20161 ggcagttgca aatgaaatat tgtcaatctt agcgcgacct tgtgcaagag cgataactga
    20221 catttcacga gcatttgttt ccttactgat acggtaacct gtgattttct tttcacgaac
    20281 ccagccgaca acagcggcta ctttttcgta actagtcatt agaaacccct ttctttttta
    20341 ttcgtgcaat ttagtataat atgttttaac ctattttgtc aagtaaaatg ttcgtttttt
    20401 accatcattt tgccctaaga gatgatagta tttgcgtcct tatgtcttcg accccctccg
    20461 gattccccgg aaggaagata gtttgattac ctccttgtgc aaaattattg agagtatcca
    20521 agtattggtt ggtcagcaag atagacataa tctgctcttc agataggcta acatttgatt
    20581 ctttcagttc acgaatcgaa tctgccagtc cgtctacaat cgccttacgt tgttgtgcaa
    20641 tacctacacc atgcaagcga tccttttcag cttctgcttc tgctgctgtg acaattttta
    20701 tcttgtcagc ttctgccaat tcttgggccg caacccgttt ccgttgagct gcgttaattt
    20761 cattcataga ttgcttaacc tcggcgtctg gctctacctt ggtaattaag gttttgacaa
    20821 taacataacc gtaggttgac atttcttctg ctacttgttt ttgtacctca agtgcaatct
    20881 catccttttt ctcgaagagc tcatccagtg tcaatttagg cacagatgaa cgcagggcat
    20941 cttcaatata ggacttaatc tgtgcttctg ggtgcatgag cttgtaatag gcatccgtca
    21001 cattattttc atttactcga tattgagttg ccacattcat tgtgacaaaa acgttatcct
    21061 gcgtcttggt ttctaccaca atttcacttt gcaacatccg taattgaatg cgggctgcga
    21121 tgacatcgac tccaaatggt attttaaagt taatacctga agtagatgtt ttttggtatt
    21181 tcccaaagcg ctcaataata gcgaccgtct gctgcttgac aacgtatagc cctgaagcaa
    21241 tcaaaatcaa agcaatcagt acaaagaaca agaaactacc aataaaaaca attggaccaa
    21301 acaccataat gacctcctaa actagcattt tataaagcga atcattttca tgaagattga
    21361 tgtactgggg atcaaactct tccaagcgag aaataaactg cgtataatct gttttatctc
    21421 ctagggcaat accgattaga accggtcctg ttcccttatt ggcacgtttg atatattcaa
    21481 aacgagtaat atcatcattt ggccctagaa tatcatttac aaattcacga agagcccctg
    21541 gacgttgtgg aaaattaaca acaaagtagt gtttaattcc ctcgtagata agcgcacgtt
    21601 cctccatttc cggcatcctg ttgatgtcgt tattaccacc agaaatgata caacaaatgg
    21661 tttgcccctt gatttggtcc ttgactacct caagagctgc aatcgttgct gctcctgctg
    21721 gctcagcaac aatacccagc ttagaataga ggtctaaaat agtcccggaa atccaccctt
    21781 catccacacc aatcaggcga tctacgtatt tccgagccac ttcgtaggtc gacttaccga
    21841 ctttctgtac cgcaataccg tcagcaaatt tatcaatttg gtctaattta accggacgac
    21901 ctttatcaaa agccgctttc attgaccgtg ccccacttgc ttcaacacca acaatcttaa
    21961 tttcaggtgc atgttctttc agataggccg aaacgcctgc aaccagacca cctcctccta
    22021 tgggaactaa aatttggtca aaactaatgg tttgctcttc cgcttcttcc aagatttcat
    22081 aggctactgt tccctgacca gcctgcacat tttcatcatc gaatgggtct ataaatgttt
    22141 tcccactttc ctgagtataa tcttgtgctg ccttggctga ctcatcgaag gtatcaccta
    22201 cgagccgaat ctctacataa tctcctccga aaaatttaac ctgccctatt ttttgttgcg
    22261 gagttgtaat gggcataaaa atcgtagcag gaatttgcat ttcctgacag gtgtaggcca
    22321 caccttgagc atggtttccg gccgaagcac agaccactcc gcgtgacttc tcatcatctg
    22381 tcaattgtga aatagcataa taggctcctc gaattttaaa agagcgaaca cgctgttcat
    22441 tttctctttt taaataaata gttgcaccat atttttccga caagtatcga ctataatcca
    22501 agggggtacg tactaccacg cgcttaagaa cggagtatgc ttgttctaca ttttttgctg
    22561 taatcatagt tccattaatt tc
//
LOCUS       DBNFCK010000021        22102 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.021, whole genome shotgun sequence.
ACCESSION   DBNFCK010000021 DBNFCK010000000
VERSION     DBNFCK010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 22102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22102
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.021"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(63..1001)
                     /gene="msrB"
                     /locus_tag="AC1PIW_000627"
     CDS             complement(63..1001)
                     /gene="msrB"
                     /locus_tag="AC1PIW_000627"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HIP6605586.1"
                     /translation="MKEIYLAGGCFWGMEGYFSQIDGILDTSVGYANGQVETTNYQLL
                     KQTDHAETLYLAYDETRINLREILLYYFRVIDPFSVNQQGPDKGRQYRTGIYYTDEAD
                     LPTIEQVMTEQSQLFGGRPLAVEVEPLAHYIPAEDYHQDYLKKNPQGYCHIDLGQAKI
                     PLIDVADYQKPDQQVLKDSLTDLQYQVTQEAATERPFENEFWNSEQAGIYVDITTGEP
                     LFLSTDKFDSGCGWPSFTKPISKEVATYFQDKSHGMNRIEVRSRAGHAHLGHVFDDGP
                     RDKGGLRYCINSAALRFIPREEMEEAGYGLFLDLLK"
     gene            complement(1041..2570)
                     /locus_tag="AC1PIW_000628"
     CDS             complement(1041..2570)
                     /locus_tag="AC1PIW_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIP6605587.1"
                     /translation="MSTISYKPLNLYRQFKTAAQEFPNVAIYFDKPYASFPELGLENT
                     YQTVFEAIQKRAAQFAAAGIGYGDKVILYKSPAFDTYLLAVAVTALGAVPVMISYHLP
                     SSNLDVFAERLEKSFIVYDQETEERVAGMTRTDLVTKIFLPQVLVQEAVAFEENLLPE
                     DVIQYMTHTSGTTGVPKLICHTAQTMGWRVAWQQTIFDKMTERGLLAFHISPVHSRYN
                     IGVSSAIGLGFPLYPLSSARKDDVERALALHRPSALETHPNNFVQWARLAKEKPEVFS
                     SIRYYHSTFDAINIGTLRAFLEASKEQDPVFMQVYGQSECGPMILRYHRLENLGTVSG
                     RDMGIGLEGYTEARITDAQGNPLPAGENGHIQFLSKGRAVTYYKEDARFQDNVYGAWW
                     DSGDYGCMTPEGTLLLKDRQVDLIEHIDSNLALEDLLLDKLDFLSEVVIIRDVNGAPQ
                     PIIALAEDAEMNWEAWWAMVADLPLLKEPILMAYDDIPRTATMKVQRLKMEAEMKEKN
                     L"
     gene            complement(2838..3152)
                     /gene="trxA"
                     /locus_tag="AC1PIW_000629"
     CDS             complement(2838..3152)
                     /gene="trxA"
                     /locus_tag="AC1PIW_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIP6605588.1"
                     /translation="MVQVITDANFEVETQEGVVLVDFWAPWCGPCRMQAPILEQLADE
                     VDEDELRIYKMDVDENPNTARQFGIMSIPTLLFKKDGQVVKQVAGVHTKDQIKAILAE
                     IG"
     gene            complement(3359..4012)
                     /locus_tag="AC1PIW_000630"
     CDS             complement(3359..4012)
                     /locus_tag="AC1PIW_000630"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6605589.1"
                     /translation="MIIRKYQTSDEKGWVYCKALSYLFSPFFDDRETEKPELMTDVYD
                     HRVEWVAEVDGQIVGLIDIDIYTEECSQSYIYAPSKRTAYFTNLAVHPDFQGQGIAQA
                     LFEKAEQELRAQGVEKLAIFTREDDAANHLYQKWGGEVVCSDYLVVGAPQDVPTFRFG
                     IDLERGRLSFSDMEGQPVPYYLREGVYVVSEEAGLDLFDIEDVYQELTYVVDLTEKS"
     gene            complement(4036..6369)
                     /locus_tag="AC1PIW_000631"
     CDS             complement(4036..6369)
                     /locus_tag="AC1PIW_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009659728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0045910 - negative regulation of DNA
                     recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HIP6605590.1"
                     /translation="MNNKIIETLEFHKVRQKIEPYLLTEQGFEELRQLEPMVEVHRIQ
                     QAFDELTDIAQIFVENPYFSLAATSDIGPAMRRLELDTDLNIAELLAVKKVLEVSKSL
                     LDFYGNLENVSLSQLDKLFEKIELFPHLQGSLQSINDAGFVEDFASEKLARIRRKIRE
                     AEDQVRQVMQDILKTKGDMLSDSILASRNGRNVLPVKNTYRNKIAGVVHDISASGSTV
                     YIEPRAVVTLNEEISHLRAEERHELNRILQELSDMLRPHGGVIRNNAWLIGHIDFVRA
                     KHLFARDHQAVVPKLSEKQDIALLNVRHPLIAKPVPNDLYFGSQLTAIVITGPNTGGK
                     TIMLKTLGLTHLMAQSGLPILADKGSRVAIFKEIFADIGDEQSIEQSLSTFSSHMTHT
                     VEILRAADQDSLILFDELGAGTDPQEGASLAMAILDDLRLRGIKTMATTHYPELKAYG
                     IETSGIENASMEFDSNSLRPTYKFMQGVPGRSNAFEIARRLGLSDIIIQSAQSWTDTD
                     SDVNRIIEKLESQTVESRQRLDKIRDVEQENYKMNRALRKLYDELNRERENELNKARL
                     EAKEIVDMALAESEDILKNLHAAASLKPHQIIEAKAELKKLAPEVVDLSKNKVLKKAK
                     IKREAKVGDDIIVTAYGQRGTLTNQLKDGRWEAQVGLIKMTLAKDEFELVKAEKAEQP
                     KKRQVHTVKRANVRGPKARLDLRGKRYEEAMMELDEFIDQALLNNLAQVDIVHGIGTG
                     VIREGVTKYLRRNKQIKEFGYAPQNAGGSGCTIVTFK"
     gene            complement(6520..7068)
                     /locus_tag="AC1PIW_000632"
     CDS             complement(6520..7068)
                     /locus_tag="AC1PIW_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HIP6605591.1"
                     /translation="MISLAIILILAWSFYIGYSRGLVLQAFYSMGSIIALVVATATYK
                     KLASFLYLWVPFANATQGSSNYYFDEKYLFDLDMVFYAGLAFLLIYVLVYAIVRFIGI
                     FVHLLEAFNPDTKTTNLISGALAVLVTFISLQIVMVLLSTIPLAMIQDKLHSSFLANI
                     MIQYTPFTSSFLKSLWLSNIAG"
     gene            complement(7065..7376)
                     /locus_tag="AC1PIW_000633"
     CDS             complement(7065..7376)
                     /locus_tag="AC1PIW_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605592.1"
                     /translation="MANLNRYKFTFGEKQLTLTTEHDNLFMEEIERIAQEKYQAIKEK
                     MPTADPETLALLLAINALSVQLTREMAFEQKEQELASVKEKVLKKNVTLIDLDELEDK
                     V"
     gene            complement(7434..8147)
                     /gene="treR"
                     /locus_tag="AC1PIW_000634"
     CDS             complement(7434..8147)
                     /gene="treR"
                     /locus_tag="AC1PIW_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HIP6605593.1"
                     /translation="MKKYQEIYNDLKEKIRTNVYPAESSLPTEQQLQEIYGVSRDTVR
                     KALAILTEGGLIQKVQGRGSMVLKQEILNFPVSGLTSYQELTNVLQLSTKTDVVSLDM
                     ITVNSSLSHLTGFEPYSKVWKVVRTRSIDGKVSVVDTDYLAVDVVPELTTAIAEKSIY
                     EYLENKLGLDIAYAQKEITVEPTNREERELMQSKDDYLVLIKSRVYLGDTQQFQYTES
                     KHKIDKFRFVDFARRKHSL"
     gene            8381..10375
                     /gene="treP"
                     /locus_tag="AC1PIW_000635"
     CDS             8381..10375
                     /gene="treP"
                     /locus_tag="AC1PIW_000635"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HIP6605594.1"
                     /translation="MGKFEKEAKQLLEAIGGKENVNAVTHCATRMRFVLADEKKANVK
                     VIEDIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNAI
                     QRVMTMLAEIFTPIIPALIVGGLILGFRNVLEGVQMEWLGQAMKDGQLVFDADGNPVW
                     NTIVQVSPFWNGVNHFLWLPGEAIFHFLPVGITWSVSRKMGTSQILGIVLGICLVSPQ
                     LLNAYAVPGTDAATIASDWSWNFGAFSIARIGYQAQVIPALLAGLALSYLEIFWRKVV
                     PEVVSMIFVPFLSLLPAIILAHTVLGPIGWTIGQWLSTVVLAGLTGPLKWLFGAVFGA
                     LYAPFVITGLHHMTNAIDTQLIADAGGTGLWPMIALSNIAQGSAVFAYFLMNRKNEKE
                     AQVSLPATISAYLGVTEPALFGVNVKYIYPFVAAMIGSSIAGLLSVTFNIQAASIGIG
                     GLPGILSIKAEYWGAFFMTMVVAIVVPMILTAVFKRTGAFSKKEETVEEVVAPTEAVV
                     ETGAAIELISPLTGQTKELSQATDPVFASGVMGQGVLIDPSEGILVAPVDGEVSVLFP
                     TNHAVGITATNGVELLMHIGMDTVGLEGKGFTAHVKQGDKVKAGDKLISFDIDVIKAA
                     GLIAETPVIVTNQTDFDTQVIGNLPRAISQGEAILTVTKN"
     gene            10456..12081
                     /gene="treC"
                     /locus_tag="AC1PIW_000636"
     CDS             10456..12081
                     /gene="treC"
                     /locus_tag="AC1PIW_000636"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HIP6605595.1"
                     /translation="MTIDKRKVVYQIYPKSYKDTTGNGVGDLRGIIEKLPYLKELGID
                     MIWLNPFYPSPQRDNGYDISDYTAVNPDFGTMADFEEMVTVGKELGIEFMLDMVLNHC
                     STDHEWFQKALAGDKYYQDFFILRDQPTDWVSKFGGNAWAPFGDTGKYYLHLFDVTQA
                     DLNWRNPHIREELFKVVNFWKDKGVKGFRFDVINLIGKDEVLEDCPINDGKPAYTDRP
                     ITHDYLKMMNNATFGSEKGFMTVGEMSATTIENCILYTAPERKELSMAFNFHHLKVDY
                     KDGQKWTIMDFDFEELKHLFHTWGEEMSVGNGWNALFYNNHDQPRALNRFVDVENFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMDDYVDVESINAYQMLLDQGHTPK
                     QAFKIIQAKSRDNSRTPMQWDASDNAGFSTGTPWLKAGKSYQTINVEQEKTGPIFTFY
                     QELIRLRKELPLISEGDYKAAYKDSQKVYAFERLLNDEKLLVLNNFFAEEVELDLADD
                     YAHGQVLISNYPDNKLGKKIILKPYQALAIQVK"
     gene            complement(12495..13466)
                     /locus_tag="AC1PIW_000637"
     CDS             complement(12495..13466)
                     /locus_tag="AC1PIW_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIP6605596.1"
                     /translation="MKNNKKMLYTSSLALSLFSTGMISTNVLAIEWAPRTVSEISPEI
                     VQEEGMMTYTVQYGDTLSAIASAMNIDMDLLAKINQIADINLIFPDTVLTTTVDQNNQ
                     VTQVEIEAPVQGNTNETVQATVDLTTNQVTVEDTVVPLDQISSVTDSAPVEEVVEQPV
                     AEAPVVEAPVEEVVEQPVVEAPVEEVVEQPVVETPQVTALSTTTTSTSAYDVGLQPQV
                     AAFRAEVANAFGITSFSGYRPGDSGDHGKGLAIDFMVPESSALGDQVAAYAVANLASK
                     NINYIIWKQRFYAPYDSIYGPAYTWNLMPDRGSITENHYDHVHVSFN"
     gene            complement(13875..14858)
                     /locus_tag="AC1PIW_000638"
     CDS             complement(13875..14858)
                     /locus_tag="AC1PIW_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIP6605597.1"
                     /translation="MEVQMKYKWATLGTGVIANELVQALQAMGGNLYSVANRTYDKGV
                     EFAQKYGIEKVYREIDEVFEDPEVDIIYISTPHNTHINYLRKALKAGKHVLCEKSITL
                     NSEELAEAIQLAEENQVVLAEAMTIFHMPIYRQLSEVVASGKLGELKMIQMNFGSYKE
                     YDMTNRFFNKNLAGGALLDIGVYALSFVRWFMIEKPNQVLSQVKLAPTGVDEQVGILL
                     SNDAGEMATIALTLHAKQPKRGTIAYDKGYIELYEYPRGQKAVITYTEDGSQEVIEAG
                     ETAKALSYEVADMEKAVAGIENTMHLAYTQDVMEIMTQLRKEWGLVYPEEM"
     gene            14943..15833
                     /gene="rnhC"
                     /locus_tag="AC1PIW_000639"
     CDS             14943..15833
                     /gene="rnhC"
                     /locus_tag="AC1PIW_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HIP6605598.1"
                     /translation="MNTVVLKASAQQKQAMMAHYDRYRQTSKNPYIEAFFKLTGASLS
                     IYTSGKVVFQGEMAEQEASRWGYEPESSGQATIPGQNLPMIGTDEVGNGSYFGGLAVV
                     AGFVRPEDHSFLKSLGVDDSKKMTDQKICQIAPLLKEKIPHQALLLSPKKYNEVIEQG
                     YNAVSVKVALHNQAIFLLLQKGVQPEKIVIDAFTSSQNYQKYLKKEANQFANPVTLEE
                     KAEGRYLAVAVSSIIARSMFLENLVQLGQLVGMHLPSGAGSKSDQVAASILKQYGMAG
                     LNQTAKLHFANTQKAQKLLK"
     gene            15843..16472
                     /gene="lepB"
                     /locus_tag="AC1PIW_000640"
     CDS             15843..16472
                     /gene="lepB"
                     /locus_tag="AC1PIW_000640"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIP6605599.1"
                     /translation="MGAIQKTKRSPLVAFLAEWGIFLLFMAAFFASRYFIWNPVSVDG
                     HSMDPTLQHQEKLIMLKTSSIDRFDIVVASETDSDGKEKLIVKRVIGMPGDTIRYEND
                     VLYVNDQKVDEPYLDEYLAAFQKDKLQEVYSYNKQFQAVAQSAEAFTQDANGYVDFTV
                     TVPEGQYYLMGDDRLVSLDSRSVGTFSRENIKGEVVFRMWPLNRIGTVD"
     gene            16713..19202
                     /gene="recD2"
                     /locus_tag="AC1PIW_000641"
     CDS             16713..19202
                     /gene="recD2"
                     /locus_tag="AC1PIW_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SF1B family DNA helicase RecD2"
                     /protein_id="HIP6605600.1"
                     /translation="MNEVYFTGTIDRIIFENPSNFYKILLLEIEETDADYDDYEIIVT
                     GTIADVIEGEDYRFYGNLVTHPKYGQQLQISRYERSKPTSAGLVKYFSSEHFKGIGRK
                     TAEKIVELYGEDTIDKILAEPEKLTQITGLSSKNMQAFVEKLRLNYGTELILAKLAEY
                     GIPNKLAFQIQDQYKEKTLQIIEENPYQLVEDVQGLGFTIADRIAENLGIASDSPQRF
                     RAGMLFSLIHRSMETGDTYVEARDLLEATLELLEKSRHTELDPAAVAKELTGLIADDK
                     VQQEGTKIFDNSLYFAEHGIHTNLTRLMGKNGFKPFPRADVEAAIAELESISSLTYDN
                     IQKEAIVQAITNPLFILTGGPGTGKTTVINGIIAVYAILHKIDLTRNREECPVLLAAP
                     TGRATRRMNELTGLPSATIHRHLGLVEGQEESYRDDYLDADFIIVDEFSMVDTWLANQ
                     LFQNISSQTQVLIVGDAEQLPSVSPGQVLADLLKIDKLPSITLERIYRQSDDSTIVTL
                     ASQIRQGALPSDFREKKADRSYFEAQNEQIPALIERIVGAAIKSGIPANEVQILAPMY
                     RGAAGIDQLNTMTQALLNPLEEGELEFLHNEQAFRQGDRVIHLVNDAEANVFNGDLGY
                     ITDLLPAKYTDSKQDEITINFDGSEVTYPRNEWYKITLAYAMSIHKSQGSEFQVVILP
                     ITRTSHRMLQRNLVYTAITRSKSKLILLGEISAFDYAVKNAGTVRKTYLVPRFQGEMA
                     EQESKESLIKKDESKTATTTQTQPPTQPDRKEQVKVVKENNQQLSLLDQDQPEKTSPQ
                     PTEYILTVDNLLTIDPMIGIEQADIEEFFKA"
     gene            complement(19241..21310)
                     /locus_tag="AC1PIW_000642"
                     /pseudo
     CDS             complement(19241..21310)
                     /locus_tag="AC1PIW_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636049.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
     gene            complement(21493..>22102)
                     /locus_tag="AC1PIW_000643"
     CDS             complement(21493..>22102)
                     /locus_tag="AC1PIW_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HIP6605601.1"
                     /translation="RLVNQFKIDLSDVWFINMDEYLSEDLHWIDVVHPLSFRGFMQKT
                     VYSQIHPTLIMPEEQRIFPDPLDIDLISNKIKELGKIDLCIGGIGLNGHIAFNEPDST
                     LTVQEFLKLESRVLPISVETKVMNCLTELKGAVEEVPNYCVTIGMSEIFQAKKIRLAV
                     FRDWHHAVIRKTVCSKPDTGFPSTLMQIHTNCSIYLNESLAN"
BASE COUNT         6236 a         5177 c         4528 g         6161 t
ORIGIN      
        1 acatacaatt ttaatacaca aaaagcctat tctttgcgaa tagactttct gcgttcataa
       61 atttatttaa gcaaatccaa aaacaggcca tatcctgctt cttccatttc ctctctcggt
      121 atgaaacgaa gggctgctga gttgatacag taacggagac cacctttatc acgcggtccg
      181 tcatcaaaga catgacctaa atgagcatga ccagcccgac tacggacttc gatacggttc
      241 atgccgtggg acttatcttg gaaataagtc gcaacttctt tgctgattgg tttggtaaag
      301 gagggccagc cgcagcctga gtcgaatttg tccgtcgaga gaaagagtgg ctcgccagtc
      361 gtaatatcga cgtagatgcc agcttgttca ctattccaaa actcattttc gaagggtctt
      421 tccgtcgcag cttcttgagt gacttgatac tggaggtcgg tcaagctgtc ttttaagact
      481 tgctggtctg gcttttggta gtccgcaaca tcaatcagag ggatttttgc ctgcccaaga
      541 tcgatatggc agtacccttg gggatttttc ttcaaataat cctgatggta gtcttcggcg
      601 ggaatgtaat gtgcgagtgg ctccacttca acagctaggg ggcgtccacc aaagagctgg
      661 gactgctccg tcatgacctg ctcgatggtc ggcaaatcag cctcatctgt gtaatagata
      721 ccagtccgat actggcgacc cttgtcaggt ccctgctgat tgacagagaa aggatcaatg
      781 acgcggaaat agtagaggag aatttcccgc aaattgatac gagtctcatc ataggctaga
      841 tagagcgtct ctgcgtggtc tgtttgtttg aggagttgat aattggtcgt ctccacctgt
      901 ccattggcat agccaacgct ggtgtcaaga ataccatcga tctgggaaaa gtagccttcc
      961 attccccaga aacaaccgcc tgctagataa atttctttca tgtgaactcc ttcttatctg
     1021 caagcgtggt ttcgtcactc ttacagattt ttttctttca tttctgcttc cattttgagg
     1081 cgttgaacct tcatagtcgc agtacgtgga atgtcgtcat aagccatcaa gatgggctct
     1141 ttcaatagtg gtaggtctgc aaccatggcc caccaagctt cccagttcat ctcagcatct
     1201 tctgccagag cgataatcgg ctgcggtgca ccattgacat cgcggataat cacgacttct
     1261 gataaaaagt ccaatttatc caacaataag tcttccaagg caaggttgct gtcgatgtgc
     1321 tcaatgaggt caacctgacg gtcttttaga agaagcgtgc cctctggagt catgcagccg
     1381 tagtcaccac tatcccacca agcaccgtac acattatctt ggaagcgggc atcttctttg
     1441 tagtaagtca cagcacggcc ttttgacagg aactggatat gcccgttttc tccagctggg
     1501 agaggattcc cttgggcatc tgtgatacgt gcttctgtgt aaccttccag accaatcccc
     1561 atatctcgtc cgctaactgt tcctaggttt tccaagcgat ggtaacgtaa aatcattgga
     1621 ccacattcac tttgaccata aacctgcata aagactggat cttgctcttt agaagcctcc
     1681 aagaaggcgc gaagcgtacc aatattgata gcatcaaagg ttgaatggta ataacgaatg
     1741 ctactaaaga cctctggttt ttccttggcc aatcttgccc attggacaaa gttattagga
     1801 tgggtttcca gagcacttgg acgatgaaga gcaagtgccc gttcaacatc atcttttctt
     1861 gcagaagaaa gtgggtagag ggggaatcct aacccaatcg ccgacgacac gccgatatta
     1921 tagcgtgaat gtacagggga gatatggaag gccagcaagc ctctctccgt catcttgtca
     1981 aaaatagttt gttgccaagc tactcgccag cccatggtct gtgctgtatg gcagattagt
     2041 tttggaacac ctgtcgtccc tgaagtatga gtcatgtatt gaatgacatc ttctggcagg
     2101 agattttctt caaaagcaac tgcttcttgc actaggactt ggggtaaaaa tatctttgtg
     2161 accaggtcag ttcgagtcat tcctgctaca cgttcttctg tttcttggtc gtagacaatg
     2221 aaagactttt ccaatcgctc cgcaaacaca tctaaattcg aagatggcaa atggtaggaa
     2281 atcataacag gaacagcacc caatgccgtt accgctactg ctaacaaata ggtatcaaag
     2341 gctggactct tatatagaat taccttgtcc ccatagccaa tacctgctgc tgcaaattgg
     2401 gcagctcttt tttgaatagc ttcaaaaact gtttgatagg tattttccaa tcctaattca
     2461 gggaaagatg cgtagggttt gtcaaaataa atggcaacat ttggaaactc ttgagccgct
     2521 gttttaaact gacgatagag atttaatggt ttgtaactaa tagttgacat atctaacctt
     2581 tctaaaatga caatgataag gttattataa catcttgtgc caaccttcac aatcttatat
     2641 cttagcaaat atcgtcattt agtttttctt ttattacttc gacgagctag gaaatatcgt
     2701 ttctatattt ccaacctccc actatctccc agatagttgg agcaaatact aaaagactat
     2761 attagaaaat aatcacaaaa gtaagtctga ttataaaaaa agcaagtagt cgatccacca
     2821 cttgctttca ttttcaatta accaatctct gccaagatag ctttgatttg atctttcgtg
     2881 tggacaccag caacttgttt cacaacctgt ccatcttttt tgaacaaaag agttgggata
     2941 gacatgatac caaactgacg agctgtgttt ggattctcat caacatccat cttgtaaata
     3001 cgcaattcat cttcgtccac ttcatctgcc aattgctcca aaattggtgc ttgcatacga
     3061 caaggaccac accatggtgc ccaaaagtca acaaggacta cgccttcttg tgtttcaact
     3121 tcaaagtttg catctgtaat aacttgaacc ataattttct ccgtttctgt ttttctacta
     3181 tcattctaca ccaaaaaaaa acagaaagcg aatttttgct acgggatgaa gcatgagata
     3241 aacgtaccta taagaatttc agttgtaaaa aataggtata gctaaactag accgcaggag
     3301 gacgaggggg gaatcacgta ccagattcat ttacaaaaag aaaaaagttg aaaatgtttc
     3361 aacttttttc tgttaaatcc accacatagg ttaattcttg atagacatct tctatgtcaa
     3421 agagatccaa gccagcttct tcgctgacta cataaacacc ttctctcaaa tagtagggaa
     3481 ctggctgacc ttccatatct gaaaaggaca atctacctcg ctctaaatca attccaaagc
     3541 ggaaggttgg aacatcttgt ggcgctccga ccactagata atccgaacag actacctcac
     3601 caccccattt ctgatataaa tggttggctg catcatcctc acgagtaaag atggctagtt
     3661 tctctacacc ttgcgctctc aactcctgct ctgctttttc aaaaagcgcc tgggcaatgc
     3721 cctgcccttg aaaatcaggg tggactgcta ggttagtaaa ataagctgtt cgcttacttg
     3781 gtgcgtaaat ataagactgg ctgcactcct cagtgtaaat atcgatgtcg attagaccaa
     3841 caatttgtcc atctacttca gccacccatt ccacacgatg atcataaacg tctgtcatta
     3901 gttcgggctt ctctgtttct ctatcatcaa agaatggaga gaagaggtag ctgagtgcct
     3961 tacagtaaac ccagcctttt tcatcactgg tttgatattt tctgataatc ataagaatct
     4021 cccatcggtt gattgctatt taaacgtcac aatagtacag cctgatccac ctgcattttg
     4081 tggggcatag ccgaactcct tgatctgctt gttgcggcgt aggtacttgg tcaccccttc
     4141 tcggatgact ccagtaccaa taccgtggac aatatcgact tgggctaggt tattgagaag
     4201 ggcctggtcg ataaattcat ccagctccat catagcctct tcgtaacgtt tgccacggag
     4261 atctaagcgg gcttttggtc ctcgtacatt ggcacgtttg actgtgtgaa cctggcgttt
     4321 cttaggctgc tctgcctttt ccgccttgac taactcaaac tcatctttag ccaaggtcat
     4381 cttaatcaag ccaacctgag cttcccaacg cccgtccttg agctgattgg tcaaggtccc
     4441 gcgttgtcca taagctgtaa cgataatgtc atcgcccacc ttggcttccc tcttaatttt
     4501 agctttcttc agaaccttgt ttttagacag atccaccact tcaggcgcca actttttcag
     4561 ctctgccttg gcttcgataa tctgatgggg cttaagacta gctgcagcat ggagattttt
     4621 gagaatatcc tcgctttctg ccaatgccat atctacaatt tccttggctt ccaagcgtgc
     4681 cttgttgagc tcattttccc gctcacgatt gagctcgtca tagagcttgc ggagggctcg
     4741 gttcatcttg taattttctt gctccacatc acgaatctta tccagacgtt gacgactttc
     4801 aaccgtctga ctttccaatt tctcgataat gcggttcaca tcgctatctg tatccgtcca
     4861 agattgggct gactggataa taatatcaga tagaccaaga cggcgggcaa tttcaaaggc
     4921 attggagcga ccaggaacac cctgcataaa cttataggtc ggacgcaggc tattgctatc
     4981 aaactccatg ctggcatttt caataccaga ggtttctatc ccgtaggcct taagctcagg
     5041 gtagtgggtg gtcgccatgg tcttgatccc ccgcagacga aggtcatcca aaatagccat
     5101 agccaaagac gcaccctcct gcggatctgt cccagctccc aactcgtcaa agagaatgag
     5161 agaatcttga tcagctgctc ttaaaatttc caccgtgtgg gtcatgtgac tggagaaggt
     5221 tgataaactc tgctcaatcg actgttcatc cccaatatcc gcaaaaattt ctttgaagat
     5281 agctacccga ctgcccttat cagccaagat aggcaaacca gactgggcca tcagatgggt
     5341 caatcccaag gtcttaagca tgatggtctt accacctgtg ttgggacctg ttatcacgat
     5401 agccgtcaac tgactaccga aataaaggtc atttggtaca ggcttagcaa tgagcggatg
     5461 ccgaacgttg agaagggcaa tatcctgctt ctcagatagt ttgggtacaa ctgcttgatg
     5521 atcacgcgca aagagatgct tagcacggac gaaatcaata tgtccgataa gccaggcatt
     5581 gttacgaatc acgccaccat gcggacggag catatccgac aattcctgca agatacggtt
     5641 gagctcatgg cgttcttctg cacgtaggtg actgatttct tcgttcaagg tcaccactgc
     5701 ccgcggctca atataaacgg tcgaacccga agctgaaatg tcatggacaa cccctgcaat
     5761 cttattgcga taggtatttt taacaggaag gacattgcgt ccattacggc ttgccaaaat
     5821 gctatcggac agcatgtcgc ccttggtctt caaaatatcc tgcatgacct gacgaacttg
     5881 atcttcagct tcacggattt ttcttcgaat acgagccagc ttttctgagg caaaatcctc
     5941 tacaaaacca gcatcattga tggactggag ggagccctgt aaatgtggaa aaagctcaat
     6001 cttctcaaag agtttatcca actggctaag actgacattt tccagatttc cataaaaatc
     6061 caagagagat ttagaaactt ccaagacctt tttaacagct aataattcag cgatattgag
     6121 gtctgtatcc aattccaaac gacgcatggc tggaccgatg tcactagtag cagccagact
     6181 gaaataggga ttttccacaa aaatctgcgc tatgtctgtc aactcgtcga aggcctgttg
     6241 gatacgatgg acttccacca tgggctccaa ctgtcgtaat tcttcaaagc cctgttccgt
     6301 caagagatag ggctcaattt tttgtcttac cttgtgaaat tcaagggttt caataatttt
     6361 attattcata tattcctact tagtcttttc cttagcttta tctcattatc aaaacaataa
     6421 aagataaaaa tatagtcttt cccactcatc aaatcaacaa cgtctggttt tgattttcga
     6481 agagtattat aaatgacgat aaaaaggata aggcctccct taccctgcta tattactcaa
     6541 ccagagagat tttaaaaagc tactagtgaa tggtgtgtac tgaatcatga tgttggcaag
     6601 aaaactactg tgcaatttat cttgaatcat cgctaacgga atagttgata gcaagaccat
     6661 gacaatctga agagaaataa aagtcaccaa gacagccaag gcgccgctta tgagattggt
     6721 cgtttttgta tcaggattaa aagcctctaa tagatggaca aatattccta tgaaacgcac
     6781 aatagcgtaa accaagacgt atatcaaaag aaaggccaat cccgcataaa ataccatatc
     6841 taggtcaaag agatatttct catcaaaata gtagttggag ctcccctgag tagcattggc
     6901 aaatggcacc cagagataaa ggaaggatgc caatttttta taagtagccg tcgcaaccac
     6961 cagggcaata atacttccca tgctatagaa ggcctgtaga actagaccac gactgtagcc
     7021 aatgtagaaa ctccatgcca aaatgagaat aattgctaat gaaatcatac cttgtcctca
     7081 agctcatcta agtcaatcaa ggtcacattc tttttcaaaa ccttttcttt gacgcttgca
     7141 agttcttgtt ccttttgttc aaatgccatc tcgcgcgtta gttgaacgga tagagcattg
     7201 attgccaaca aaagcgcgag ggtttcaggg tccgccgtcg gcatcttttc tttgatggct
     7261 tggtattttt cctgtgcaat acgctcaatc tcttccatga ataaattatc atgctcagtt
     7321 gtcaaagtta attgcttctc gccaaatgta aatttatatc gatttaagtt tgccatatct
     7381 tacctcttct tctatctatt atagcgtaaa aaacttgatt tggctaatct ctcctataaa
     7441 gaatgctttc tacgagcaaa atctacaaag cggaatttat caattttatg cttgctttct
     7501 gtatattgga attgttgggt atcaccgaga tagacacgag atttaatcaa gaccaaataa
     7561 tcatccttcg attgcattag ctcacgctct tctcgattgg tcggctctac cgtaatctct
     7621 ttttgtgcat aagcaatatc aaggcctaac ttattttcta ggtattcgta gatggatttt
     7681 tcagcaatag cagttgtcaa ctctggcacg acatcgacag caagataatc tgtatccaca
     7741 acggagacct taccgtcaat cgaacgtgta cggacaactt tccacacctt gctatacggc
     7801 tcaaagcctg tcaagtgcga aaggctacta ttaacggtaa tcatatctaa gctgacaaca
     7861 tctgtcttgg tagaaagctg gagaacattt gtcaattcct gataggaagt taaacctgaa
     7921 actgggaaat tgagaatttc ttgcttaagg accattgaac cacgcccttg cactttttga
     7981 atcaaacctc cctcagtcaa aatcgccaac gccttacgaa ccgtatcacg actaacacca
     8041 tagatttcct gaagctgctg ttctgtcggt agggagcttt ctgccggata aacatttgtc
     8101 cgtatttttt cttttaagtc attataaatt tcttggtatt ttttcatcat ttcctcgcaa
     8161 attcatctgt caaataacat acaactatca acattataac aaaattcatt tcaaaattaa
     8221 aaaaaactaa gcaaaaacgc ttgcattatt ttttgaaatc ggatacaatg ataacacaaa
     8281 agaaatttgt ccgtacaaat ttcaaaaaag gacatgcttc gagagatgtc taaaaaatat
     8341 agggtgacgg attccataag ccgtctagaa ggaaaaatat atgggaaaat ttgaaaaaga
     8401 agccaaacag ctcctagaag ctattggcgg aaaagagaat gtcaatgcag taacacactg
     8461 tgcaacgcgc atgcgctttg ttcttgcaga tgagaaaaaa gcaaacgtaa aagtcatcga
     8521 agacattcca gcagtaaaag gaacatttac caacgctggt caattccaag tcatcatcgg
     8581 taacgatgta ccaatcttct acaatgactt tacagctgtg tctggtatcg aaggcgtatc
     8641 aaaagaagct gcaaagtcag ccgctaaaag taaccaaaat gccatccaac gtgtcatgac
     8701 catgctggcg gaaatcttca caccgatcat tcctgccctt atcgtcggcg gtttgattct
     8761 cggtttccgt aacgtcttgg aaggtgttca gatggaatgg cttggacaag ctatgaaaga
     8821 tgggcagttg gtcttcgatg ctgatggtaa ccctgtatgg aacaccatcg ttcaggtttc
     8881 tcctttctgg aatggtgtca atcacttcct ctggttgcca ggtgaagcca tcttccactt
     8941 cttgccagta ggtatcactt ggtccgtatc tcgtaaaatg ggaacttcac aaatccttgg
     9001 tatcgtcctt ggtatctgtt tggtttctcc tcagttgctc aacgcttatg ctgtacctgg
     9061 tacagatgca gcaaccattg ccagcgactg gtcttggaac ttcggtgcat ttagtattgc
     9121 ccgtatcggt tatcaagcgc aagttatccc tgccctcctt gccggtttgg cactgtctta
     9181 cctagaaatc ttctggcgta aggttgttcc agaagtggtc tcaatgatct tcgtaccatt
     9241 cctatcactt cttcctgcaa tcatcttggc tcacactgtc cttggtccta tcggttggac
     9301 aatcgggcaa tggttgtcaa cagttgtatt agctggtttg actggcccat tgaaatggct
     9361 cttcggtgct gtattcggtg ccctttatgc gccattcgtt atcactggtc ttcaccacat
     9421 gaccaacgcc atcgataccc aattgattgc ggatgctggt ggtactggtc tctggccaat
     9481 gatcgccctt tctaacattg cacaaggttc tgcagtattt gcttacttcc tcatgaaccg
     9541 taaaaatgaa aaagaggcac aagtttctct tcctgcaaca atttctgctt accttggtgt
     9601 tactgaacca gccctctttg gtgtcaacgt aaaatacatt tacccatttg tagctgctat
     9661 gattggttca agtatcgcag gcttactctc tgtaaccttc aacatccaag cagcttctat
     9721 cggtatcggt ggtcttccag gtatcttgtc tatcaaggct gaatactggg gtgcattctt
     9781 tatgacaatg gttgttgcga ttgttgtccc aatgattttg acagctgttt tcaaacgtac
     9841 aggtgcattt tcaaaaaaag aagaaactgt tgaagaagtt gtagctccaa ctgaagcagt
     9901 tgttgaaact ggcgctgcta tcgaattgat tagcccactt actggtcaaa ccaaagaatt
     9961 gtcacaagct actgatcctg tatttgcatc aggtgtcatg ggccaaggtg tccttattga
    10021 cccgagcgaa ggtatcttgg ttgctccagt cgatggtgaa gtatctgtcc tcttcccaac
    10081 taaccatgct gttggtatca ctgctacaaa cggagttgag ctccttatgc acattggtat
    10141 ggatactgtt ggattggaag gcaaaggctt cacagctcac gtcaaacaag gcgacaaggt
    10201 aaaagctggt gataaattga ttagctttga catcgatgtc atcaaggctg ctggtctcat
    10261 cgctgaaaca ccagttatcg tgaccaacca aactgacttt gacactcaag ttatcggaaa
    10321 tcttcctcgt gccatctcac aaggcgaagc tattctgaca gttacaaaga attaatgttc
    10381 taggagggcg caaggtaact tgcacctcct atcattgctt atgcaagaag gaaaaatcaa
    10441 gaaagagaga gcaaaatgac aatagacaaa cgaaaggttg tttatcaaat ctacccaaaa
    10501 tcttacaagg atacaactgg aaatggtgta ggggatttac gaggaattat tgaaaaactc
    10561 ccatatttaa aagagctagg tattgatatg atttggctca atcccttcta cccaagtcct
    10621 cagcgggaca acggctatga tatttctgat tatacagctg tcaatcctga ttttggcacc
    10681 atggctgact ttgaagaaat ggtgactgtc ggcaaagaac taggcatcga attcatgcta
    10741 gacatggtcc tcaatcactg ctcaaccgac cacgaatggt tccaaaaagc ccttgccggc
    10801 gacaaatatt accaagattt ctttatcctg cgtgaccagc caactgactg ggtgtccaag
    10861 tttggtggca atgcctgggc tccttttgga gatactggga aatactacct ccacctcttt
    10921 gacgtgaccc aagccgacct caactggcga aatcctcata tccgtgaaga actcttcaag
    10981 gtggtcaact tctggaaaga caagggtgtc aaaggcttcc gcttcgacgt tattaacctg
    11041 attggtaagg acgaagtcct agaagattgc ccaattaatg acggcaagcc agcttacacc
    11101 gaccgcccga ttacccacga ttacctcaag atgatgaaca atgctacttt tggtagtgaa
    11161 aagggcttca tgaccgttgg ggaaatgtct gccaccacca ttgaaaactg tatcctctac
    11221 acagctcctg aacggaaaga attgtccatg gccttcaact tccaccactt gaaagtggat
    11281 tacaaggacg ggcaaaaatg gaccatcatg gactttgact ttgaagaact caaacacctc
    11341 ttccacactt ggggtgaaga aatgtccgtc ggaaatggct ggaacgccct tttctacaac
    11401 aaccacgacc aacctcgtgc cctaaaccgc tttgttgatg tggagaattt ccgcaaggaa
    11461 ggagcaacca tgttggcagc ctctatccac ctatcacgcg gcacacctta catctacatg
    11521 ggtgaggaga tcggtatgat cgaccctgac tacgatagca tggacgacta cgtggacgtg
    11581 gagtctatca atgcctacca gatgctcttg gaccaaggtc acacgcctaa gcaggccttc
    11641 aagattatcc aggccaagtc ccgtgacaat tcccgcaccc cgatgcaatg ggacgcttct
    11701 gacaatgcag gcttctcaac aggcactcct tggttgaaag ctggcaaatc ctaccaaacg
    11761 attaacgttg aacaggaaaa aacaggccca atctttacct tctaccaaga actcatccgc
    11821 ctgcggaaag aactccctct gatttcagaa ggggactaca aggcagccta caaggatagc
    11881 cagaaagtct acgcctttga acgcttgcta aatgacgaaa aactgttggt cctcaacaat
    11941 ttctttgcag aagaggtcga attagacctg gcagacgact atgcccacgg ccaagtcctc
    12001 atcagcaact atcctgacaa caaactaggc aaaaaaatca ttctcaaacc ttaccaagca
    12061 ttggcgattc aagttaaata aacgataaaa catccgaaaa taacactcta catgtcaatt
    12121 ttaattttcc taaaattaga acccgccttc acagctcagt aggttacttg tgaaggcaag
    12181 ttcttggttt aaggtttctt ttaggaatat attttagact gtaatttgcg cacgaaacag
    12241 ctatctgggg ggagatagtt gtttcttctt ttatagtcat ttgaattaac tttgatacat
    12301 cgtcactttc ggcttgccgt actctacgaa agtgacttgt ttcgcaagtc ttatttccaa
    12361 cctagaacag cctctaggct gttctagcta cctgcacctc agttccttgt ctgaaagtta
    12421 attcattcga ctatattact acatataaag caataaccgt ttcagataag gctgaaacgg
    12481 ttatttttgt aagtctaatt aaaagataca tgcacatgat cgtagtggtt ttctgtaatg
    12541 ctaccacggt ctggcatcag gttccatgta taggctggac catagatact gtcatacggc
    12601 gcatagaagc gctgtttcca aatgatgtag ttgatatttt tagaagctaa gtttgcaact
    12661 gcataagccg ccacttgatc tcctagagct gagctctcag gcaccataaa gtcaattgcc
    12721 aatcccttac catggtcgcc agaatcacca ggacggtaac ctgagaaaga agtaataccg
    12781 aaggcattag ctacttctgc gcggaaggct gctacctgag gttgcaaacc gacatcataa
    12841 gcacttgtac ttgttgtagt ggttgatagg gctgtcactt gtggagtttc aactacaggc
    12901 tgttctacaa cttcctctac aggtgcctca actacaggct gctctacaac ttcctctaca
    12961 ggtgcctcaa ccacaggtgc ttctgctaca ggctgttcta caacttcctc tacaggcgct
    13021 gagtcggtaa ctgatgaaat ttgatccaag ggaacaaccg tatcctcaac cgttacttga
    13081 ttggttgtta ggtcaacagt tgcctgaacg gtctcatttg tgtttccctg aacaggagct
    13141 tcaatctcaa cctgagtcac ttgattgttt tggtcaacag tcgtcgtcag tactgtatca
    13201 gggaaaatca agttgatatc tgcaatttga tttattttcg ccagcaagtc catatcaata
    13261 ttcatagctg aggcgatggc agataaggta tctccatact gaacagtata ggtcatcatt
    13321 ccttcttctt gtacaatttc tgggctaatt tcagaaacag tacgtggagc ccattcgata
    13381 gctaaaacat ttgtcgaaat catccccgta ctaaagaggg aaagagccaa tgaagatgtg
    13441 tataacatct tcttattgtt tttcatgatg gatgtttttc tcttgcgacg catatgttgt
    13501 ggtttatttg ttctcttacg tttcataatc ttattccttc attatttttg agatggttct
    13561 atcttaccta aattaaatct gaaaaacaag ccttcaaccg ttcttttaaa taaactgtct
    13621 ccactatgta atcttaggac ttttctgtca cacaatcaat ataaatgtca tctgactgtc
    13681 atattattag gtaatactca atgaaaatca aaaacagact cggaaacgca gatgcagata
    13741 gaacaaaact tcatcaaatc aagtcaacaa cgtctggatt ttcgaagact ataagtaaaa
    13801 aataagagta aggcagtaat ccaacaattg gctactaact cactctcatt tctattttcg
    13861 gattagtcaa gatcctacat ttcttcagga taaaccaagc cccattcttt acggagttgg
    13921 gtcatgatct ccatgacatc ttgggtgtag gctaggtgca tggtgttttc gatgcctgcg
    13981 acagcttttt ccatatccgc cacttcataa gaaagagcct tggctgtctc gcccgcttcg
    14041 attacttctt gactaccgtc ttcagtatag gtaatgactg ctttctgacc acgtgggtac
    14101 tcgtaaagct cgatgtagcc tttatcgtag gcaatagtgc cccgttttgg ctgtttggca
    14161 tggagggtca gggcgatggt cgccatttct cctgcatcat tgctgagcaa aatccctacc
    14221 tgctcgtcca cacctgtcgg agccaatttg acctgagata ggacttgatt tggcttttct
    14281 atcataaacc aacggacaaa ggacagagcg tagacaccaa tatccaagag ggcaccgccg
    14341 gccaagttct tattgaaaaa gcggttggtc atgtcatatt ccttgtagct gccaaagttc
    14401 atctgaatca tcttcaactc tccaagcttg ccactggcaa ccacttcgct caactgacga
    14461 tagattggca tgtggaaaat ggtcatggcc tccgccaaga cgacctgatt ttcctcagct
    14521 aattgaatag cttctgctaa ttcttctgaa ttaagcgtaa tagacttttc acagaggaca
    14581 tgctttccag ccttcaaggc ctttctcaaa taattgatat gggtattgtg aggcgtagaa
    14641 atatagataa tgtctacttc aggatcctca aacacttcat cgatttctcg ataaactttc
    14701 tcgatgccat atttttgcgc aaattccaca cccttgtcat aggtacggtt ggctaccgaa
    14761 taaagatttc ctcccatagc ctgcaaggcc tgaaccaatt cgttggcaat gacacctgtt
    14821 cccagagtcg cccatttata cttcatttgc acttccatag tgtacctcac tttcttctac
    14881 cattatagta aattccaccc ttttctgcac actctaacta gcatttgtgt tacaataaga
    14941 acatgaatac cgttgtacta aaggcatctg cccagcaaaa acaagccatg atggctcact
    15001 atgaccgcta tcggcagacc agcaaaaatc cttacataga agcatttttc aaactgaccg
    15061 gtgcaagcct gtccatctac acttctggaa aagtcgtttt tcagggggaa atggcagagc
    15121 aggaagcaag ccgttggggc tatgagccag aaagctctgg acaggcgacc atacctggtc
    15181 aaaacctgcc catgattggt accgatgagg ttggaaacgg gtcttatttt ggaggtttgg
    15241 cagtcgttgc tggttttgtc cgcccagaag accattcctt tctcaagtcg cttggtgtcg
    15301 atgattccaa aaaaatgaca gaccaaaaaa tctgccaaat cgcccctctc ttaaaagaga
    15361 aaatccccca ccaagctctg ctattgtcac cgaaaaaata caacgaagtc atcgagcaag
    15421 gctacaatgc ggtgtctgtc aaggtcgccc tacacaatca ggctattttc ctactcttgc
    15481 aaaagggtgt ccagcctgag aagattgtca ttgacgcctt cacttccagt caaaattatc
    15541 aaaagtactt gaagaaggaa gccaaccaat ttgccaaccc cgtcactcta gaagaaaagg
    15601 ctgagggcag gtatctggct gtggcagtat cttctatcat tgcaagaagt atgtttttag
    15661 aaaatcttgt ccagcttggt caactagttg gaatgcatct gccgtctggt gcgggcagta
    15721 aatccgacca agtcgcagca agcatcttaa aacaatatgg tatggctggt ctcaaccaaa
    15781 ctgccaaact ccactttgcc aatacgcaaa aagcacaaaa attattgaaa tgaggaaaaa
    15841 atatgggagc aatccaaaaa acaaaacgct caccactagt cgccttctta gctgaatggg
    15901 gcatcttttt actgtttatg gcagcttttt ttgccagtcg ttactttatc tggaatcctg
    15961 tatctgtcga tggccattcc atggacccaa cgcttcaaca ccaagaaaaa ctcatcatgc
    16021 tcaaaacatc ctctatcgac cgctttgata ttgttgtagc cagcgaaaca gacagcgatg
    16081 gcaaggaaaa actgattgtc aaacgtgtga ttggtatgcc aggtgacacc attcgctacg
    16141 aaaacgatgt cctctatgtt aacgaccaaa aagtggacga gccttactta gatgaatatc
    16201 tagctgcatt ccaaaaagat aaacttcaag aagtctattc ttataacaaa caattccaag
    16261 ctgtggccca atctgctgaa gctttcacac aagatgccaa tggttacgta gactttaccg
    16321 tgacagtccc agaaggacaa tactacctga tgggagatga ccgccttgtc tcactagata
    16381 gtcgtagtgt aggaacattt tcccgtgaaa acatcaaagg agaagttgtc tttcgcatgt
    16441 ggcctctcaa ccgtattggg actgttgatt aatacaaaca agtttggagc aaaaagcaca
    16501 gcaccacttc tcagagttcg tgtcaacatc ccagcgcagt ggttgattgg gtttaacagt
    16561 ccactggact gttggagctg tgcgggttgg gagtaaaata gtccagtgga ctattttagc
    16621 ccgaacctgc atatttggaa gtgaggggaa ctctttttgg tctatgcgag ttcttcccca
    16681 ctcccttttc attattattg gaaataaact ctatgaacga agtgtatttt accggtacta
    16741 ttgaccggat tatttttgaa aatcctagta atttttataa aatccttctc cttgaaatcg
    16801 aggagacaga cgcagactat gatgactacg aaattattgt gaccggaacg attgccgatg
    16861 tcattgaagg agaagactat cgtttctatg gcaatctggt cacccatccc aagtatggtc
    16921 agcagctgca aatttcgcgt tacgaacgca gcaagcctac ctcagctggt ctggtcaagt
    16981 atttctccag cgagcatttc aagggaattg gacgcaaaac agctgaaaaa atcgttgagc
    17041 tctatggcga agataccatt gataagattt tagctgaacc tgaaaaacta acccaaatca
    17101 caggcctatc cagcaagaac atgcaggcat ttgtcgaaaa actccgcctc aattatggaa
    17161 ccgagttgat cttggcaaaa ctagccgagt atggcattcc caataaattg gcctttcaaa
    17221 tccaagacca gtataaggaa aaaacacttc agattattga agaaaatccc tatcaactgg
    17281 tcgaagatgt ccaggggctc ggctttacca ttgccgatag gattgcggaa aatctaggaa
    17341 ttgccagtga ttcaccccaa cgttttcggg ctggcatgct ctttagtctc attcatcgct
    17401 ccatggaaac aggcgatacc tatgtggagg ccagagactt gctggaagca acccttgaac
    17461 tgctggaaaa atcccgccat acagagctgg accccgctgc tgttgccaaa gaattgacgg
    17521 gactgattgc agacgacaag gtacagcaag aaggcacaaa gatttttgac aacagcctct
    17581 actttgccga acatggtatc catacaaacc taactcgcct gatgggaaaa aacggcttca
    17641 agcccttccc acgcgcagac gtcgaggctg ccatagcaga gctggaaagc atatcttctc
    17701 tcacctacga taacattcaa aaagaagcca ttgtccaagc cattaccaat ccgcttttca
    17761 ttctgacagg cgggccaggg actggaaaaa caacggttat caacggcatt atcgcagtct
    17821 atgctatctt gcataagatt gacctgacgc gaaaccgaga agaatgccca gtcctcctag
    17881 ccgctccaac tggacgagca accagacgga tgaatgaatt gacagggctg ccttccgcca
    17941 ccatccaccg ccatctcggt ctggtagaag gacaggagga atcctaccgt gatgattatt
    18001 tggatgccga ctttatcatc gtggatgagt tttctatggt agatacttgg ttggcaaacc
    18061 agctcttcca gaatatctcc tcccagaccc aagttctgat tgtcggcgat gccgagcaat
    18121 tgccttctgt cagccccggt caagtacttg ctgacctctt gaaaattgac aagctgccca
    18181 gcattactct agaacgtatc tatcgtcaat ccgatgattc gaccattgtt accctagcca
    18241 gccagattcg acagggtgct ctgcctagcg attttcgtga gaaaaaggct gaccgttcct
    18301 attttgaagc ccagaatgaa caaattccag ccctgattga gcgcattgtc ggcgcagcca
    18361 tcaagtcagg tattcctgca aacgaggtac agatcctcgc tcccatgtac cgtggggccg
    18421 caggcatcga ccaactcaac actatgaccc aggccctgct caatccactg gaagagggag
    18481 agttagaatt cctgcataac gagcaagcct tccgccaagg cgaccgggtc attcatttag
    18541 tcaacgatgc cgaggccaat gtcttcaatg gtgatttagg ctacatcacc gacctccttc
    18601 ctgccaagta caccgactcc aagcaggacg aaattaccat caacttcgac ggtagcgagg
    18661 tcacctatcc ccgaaacgaa tggtacaaga ttaccctggc ctatgccatg tccatccaca
    18721 agtcccaagg cagcgagttt caggtagtca tcctccccat cacccgcacc agccaccgta
    18781 tgctccagcg caatctggtc tacaccgcca tcacccgctc caagagcaag ctcatcctcc
    18841 tgggcgaaat ctccgccttc gactacgccg tcaaaaacgc cggcaccgtc cgaaaaacct
    18901 atctggtccc acgtttccaa ggggaaatgg cagagcagga aagcaaggaa agcctgatta
    18961 aaaaagacga aagcaaaaca gctacaacca cgcaaaccca gccccctact cagccagaca
    19021 gaaaagaaca ggttaaagta gtcaaagaaa acaaccaaca actctccctt cttgatcaag
    19081 accagccaga aaaaacaagt ccccaaccca cagaatacat tttgactgta gacaacctac
    19141 taaccatcga ccccatgatt ggcatagaac aggcagatat tgaagaattt tttaaagcat
    19201 aaaaaccagc aagtcagtag atgaacaccg ttatattctt ttatttgaca gcctctaaag
    19261 ctgctttata atctgggtgt tcgttgacat tgtctaggta ttctacgtag acgatctcat
    19321 tgtcggcatt aaggacaaag accgcacgcg ccagcaaatt ccactcccgc atgagcacac
    19381 cgtaggattt tccaaaggca tggtcatagt aatcagacag ggtgatgcca tcccctacca
    19441 tggcgactgt cttgccctgt tcttgtaaga acttgacttg gttggccttg tcatctggca
    19501 ggacctgact gaccacctgc tcaatgccga cttccttggc aatagcctgg gcagtttttt
    19561 cattatctcc tgtcagcatg acaacctcta gttccatggt ttgcagagcc tggacagcct
    19621 gacggctggt ctcttttact ttatcagcaa tggcaatgac tgctagtaat tcttgctggc
    19681 tggctaggaa aacaggcgtc ttagcttgat gggcaaatgt ttccgcaact gcacgtcctt
    19741 tggaaacatc aattccctgt tcccgcatca accgttcatt tcccagatag atggtctgct
    19801 ctgctattgt taccgaaaga ccacgaccag aaagtgcctg aaaatcttgg ctggcaagta
    19861 agggaattga ttcctcttct gctgaacgta agagggcttg agccaatgga tgctcggagt
    19921 attgttcact gcttgctgcc agttggagaa cttgctcacg atttttagta gacaaaaggt
    19981 ggatgtctgt cacttgtggc ttgccctcag taatggtccc agtcttgtcg aagacaatgg
    20041 tgttcacacc ttgtagggtt ttaatagttt gaccagactt gaacaaaaga ccattttctg
    20101 ccccttttcc agtcccaacc atgatagcgg ttggtgtggc aaggcccaag acacacgggc
    20161 aggcaatgac aaggacggag atggtaatgg aaagtgagaa catctagggc tcttgtccca
    20221 agaagtacca agcaaggcca gaaaggacgg ctaatcccat tacaattgga acaaagactg
    20281 ctgaaacgcg gtcagccaac tgcgcaatcg gagcttttga cccctgtgct tcttccacca
    20341 aacgaataat ttgggacaat gtggtatctg ctccaacttt tgtcgcttcc atttggaaag
    20401 caccatgttg attgagagtc gcaccaacga ctgtatcccc gatgtttttt taacaggcaa
    20461 actttcccct gtcagcatgg attcatcaat tgtcgattgg ccttctacta ttatcccatc
    20521 aactggaatc tgttcaccag ggcggacttg aaggacatct cctactttga cttggtctaa
    20581 ggggagggtc acttcttgac cttcacagag aacgcgtgca gttttagggg cgagattcat
    20641 cagtttactg atggcttctg aagtacgctc ttttgataat gcctccatgt attttccaag
    20701 agtgataagg gttaaaataa cagctgccga ttcaaaatag agctcaggat gtccatgttg
    20761 ggtggctact tggttggtag caatcaaaat cgtcataatg atcccttgaa acaaggcagc
    20821 acttgtccca acggcaatca aactatccat attgggatga ccggcaaaca gggttttaaa
    20881 ccctttttga taaaatgcac gaccaacata taggactggt aatacaagaa tgagctgaac
    20941 tagggcaaaa actagcggct ggtgtaagat agctggcaat ggcaatcccc cacctggtaa
    21001 cataggtccc attgcaatgt agagtagtgg aagcgtgaaa atggttgacc agataaatct
    21061 gcgccataaa tcatttttgt gttgttcttt tccgcttggt tgagcttctg actcttgtgt
    21121 ttttggcagt accgctttat aacctgcttt ttcaacagct gtcgatatct gataagctca
    21181 gatgggctgg atcatactga atgtttgttt tttccgttgc tagattcaca ctggcgtttt
    21241 gtattccatc tagcttattc aaggctttct cgactgtcag agcacaggtt gcgcaagtca
    21301 tcccattgac attatagatg gatgttttca taatactgct ctccttttga ctacatttgc
    21361 agtcaaatca catgcttttt gattacattt gtaaacgatt ttgattaaaa aaatgagaga
    21421 atcctctctc attaggtatc tgatcaaaag ggtaacagac ttatatatgt ttcatatgtg
    21481 tttgaacatc cgttaatttg ctaacgattc atttagatag atagaacaat ttgtatggat
    21541 ttgcattaga gttgatggaa atccagtatc tggcttagaa caaactgttt tacgtatgac
    21601 cgcatgatgc caatcacgaa aaacagcaag tcgaattttt ttcgcttgaa aaatctcgct
    21661 cattcctatt gttacacaat aattaggcac ttcttctaca gcccctttta attccgtcaa
    21721 acaattcata acctttgttt cgacagagat aggtaacact cgcgactcta attttaaaaa
    21781 ttcttgaact gttaatgttg aatccggttc attaaatgca atgtgcccat ttaggccaat
    21841 tccaccaata cataagtcga ttttacctaa ctctttgatt ttatttgaaa tcaagtcaat
    21901 atcaagagga tctggaaaaa ttctttgttc ttcaggcata ataagagttg gatgtatttg
    21961 actgtagaca gttttctgca taaatcctcg aaaactaaga gggtggacta cgtctatcca
    22021 atgtaaatct tccgataaat attcatccat gtttataaac caaacatcag acaaatcaat
    22081 cttaaattga ttcactaatc ta
//
LOCUS       DBNFCK010000022        21487 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.022, whole genome shotgun sequence.
ACCESSION   DBNFCK010000022 DBNFCK010000000
VERSION     DBNFCK010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 21487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21487
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.022"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..229
                     /locus_tag="AC1PIW_000644"
     CDS             <1..229
                     /locus_tag="AC1PIW_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HIP6605602.1"
                     /translation="MTTVPTPLGNNVRVYDFERIICDFVIHREKIDSELFVKTLQSYG
                     NYPKKNLAKLYEYATKMNTLEKVKQTLEVLI"
     gene            226..1071
                     /locus_tag="AC1PIW_000645"
     CDS             226..1071
                     /locus_tag="AC1PIW_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HIP6605603.1"
                     /translation="MNKAKLTALCHKISKNTGLTFNSVMTYYFLEVILKKLSQSSYSN
                     HYIFKGGFLLSNVIGVESRSTVDIDFLFHQITLSEETVKQQLKEILADSEEGISFVIQ
                     SITAIKESDDYGGYRATISCQLENIKQVIHLDIATGDVVTPQPITYDYKAIFDEDNFP
                     IIAYTIETILAEKLQTIYSRNFLNSRSKDFYDVYILSKLKKEDIDFIQLRNACQRTFS
                     YRETELDFVKIIELLERFKSDPIQNKQWQNYSKKYSYTKGISLADVLDEMISLITIII
                     STDSD"
     gene            complement(1157..1441)
                     /locus_tag="AC1PIW_000646"
                     /pseudo
     CDS             complement(1157..1441)
                     /locus_tag="AC1PIW_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
     gene            complement(1555..2565)
                     /locus_tag="AC1PIW_000647"
     CDS             complement(1555..2565)
                     /locus_tag="AC1PIW_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HIP6605604.1"
                     /translation="METIIEKMKKESKTGLTSGDLVNHIFKNHKIDIQDGDSVTLLNM
                     GYYHGYKRYRFVKKSNSDGILPLSSFKEIEAIYNFDMELKGLFYPIVMLVETALKNRT
                     IDSIVAESSSGIEDILEHQLACYMDYPEDHEKYGNKLDQYNETRQNVLDTIDYYSDKN
                     EVIKHYLKSDYQIPLWAYFEVITLGQFSRFLGSLTKSSREKLNQDFTIIPNRSNSLEL
                     VVNALIELRNATMHNSAIFDANFSSGVSKALKKQIKQDLGLSTFTCVFIEDYFILLIW
                     TLNILKFDTVVLNNYINQFNEIVERFDVSLNNRDILFKIISTNRKTSIQQLSKFIDEK
                     RN"
     gene            complement(2702..5503)
                     /locus_tag="AC1PIW_000648"
     CDS             complement(2702..5503)
                     /locus_tag="AC1PIW_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HIP6605605.1"
                     /translation="MKEDKKVVKQARKNFRSNLKSARMHYRKEVRTLRQTVPKKGRFR
                     KPAQNSLFQEKKTELKENLLSSQKEAEEKFLKEITYVSPRLLKVKEIKNYRLPHAQER
                     LRTARKHLSEVKLSEKQQAVDSKFTFQKENPSLKSRFQFHQEKSFDRLSAEKEVSSAK
                     REVKQLKKVQKSKKSSTKVRAGLGLAASESLDLIAQEDDLDGLRTLKDTSLKVRRYGR
                     FTYQAGKVAVKSGQTGVRFTKTKFSHGKERFQNFKKGKGFTRQKPLKPRRRYQTFLKL
                     ARKQSVAGFKGIVQAIKGSMTFFSVLAGNPLTWIISGILLILLLMMSFFMSVSGSSVI
                     QQDEIELSKSYTHMTLEDAEHTRTNEKGITFYTKIDEVMVYMNHQYQDYKLDDFMETG
                     GTTYKAFLSQVWTDLNGGDSIKSMSDLYKEPAYKLSDEDQEELKELTEEGNYLALQEL
                     DNPFQGQTDEDSLNMTYRYGYEVIDEKPTLHHHIILEAKEGQVIVAPMDGKVSLDGEN
                     VVLTSGKGVNKTKLTLFGIHSGRVSEHQHVLAGDIIGQTKDGTGLKVTYQKVDDDTDK
                     LVNVNPAFYFPKVIQVQTTILPTIGQFGGDEFERAKAIYDYLKSKGATNQAIAAILGN
                     WSVESSINPKRAEGDYLSPPIGATDSSWDDEGWLSLNGPTIYNGRYPNILKRGLGLGQ
                     WTDTADGSRRHTLLLEYAKGKHQKWYDLGLQLDFMLHGDSPYYTNWLKDFFKNSGSPA
                     SLAQLFLIYWEGNSGDKLLERQTRASEWYYQIEKGFSQPNGGTAQSDPKALEAVRGDL
                     FENSVPGGGDGMGYAYGQCTWGVAARINQLGLKLKGKNGEKIPIISTMGNGQDWVRTA
                     SSLGGETGTSPQAGAILSFAGGGHGTPTEYGHVAFVEKVYPDGSFLISETNYNGNPNY
                     TFRKLSGVDSSLSFAYTTK"
     gene            complement(5505..7856)
                     /locus_tag="AC1PIW_000649"
     CDS             complement(5505..7856)
                     /locus_tag="AC1PIW_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HIP6605606.1"
                     /translation="MKKLKHSMKPKTSSNDKKQKTKTQKQEIRPSTVNTLAYQGLFQN
                     GLMQVSPSYFSQTYLLGDVNYQTVGLDDKGAIVEKYSDLINSLDDKTNFQLTIFNQKV
                     NLEKFRKSILYPLQEDGFDAYRDELNRMMDSNLEAGENNFSAVKFLSFGKSDQTPKLA
                     FRSLSQIGEYFKSGFSEIDVSLGLLGGEERVNVLADMLRGENHLPFSYKDLTLSGQST
                     KHFIAPTYLSFKHKNHIELDDRLLQIVYVRDYGMELGDKFIRDLMQSDLEVMISLHAK
                     GSTKSETMIKLRTKKTLMESQKIGEQQKMARTGIYLEKVGHVLENNIDEAEALLQTMT
                     QTGDKLFDTVFLIGVLADTEDQLKQSLDIIKQVAGSNDMIIDNLTYMQEAAFNSLLPF
                     GKNYLEGVSRSLLTSNIAVNAPWTSVDIQDKGGKFYGINQISSNIISIDRGKLNTPSG
                     LILGTSGAGKGMATKHEIITTKLKEADSDTEIIIVDPENEYSIIGQAFGGESIDIAPD
                     STTFLNVLDLSDENMDEDPVKVKSEFLLSWIGKLLDRKMDGREKSLIDRVTRLTYKHF
                     DTPSLVEWVFVLAQQPEQEAKDLALDMELYVEGSLDIFSHRTNIKTDSHFLIYNVKKL
                     GDELKQIALMVIFDQIWNRVVKNQKLGKKTWIYFDEMQLLLLDKYASDFFFKLWSRVR
                     KYGAIPTGITQNVETLLLDANGRRIIANSEFMILLKQAKSDREELVHMLGLSKELEKY
                     LVNPEKGAGLIKAGSTVVPFKNKIPQHTKLFDIMSTDPEKMRT"
     gene            complement(7813..8166)
                     /locus_tag="AC1PIW_000650"
     CDS             complement(7813..8166)
                     /locus_tag="AC1PIW_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HIP6605607.1"
                     /translation="MNTRVFKDISKVQHRAWLGFTTRQVIFVFPAIAITILVLGLNLF
                     YWQFGDWFVYGFVFSFTIPLMLFGVYRPNDLPFEIYLKYCFHYELTVPDRTFTGRKGD
                     QREKIKTLNETKDLF"
     gene            complement(8228..9082)
                     /locus_tag="AC1PIW_000651"
     CDS             complement(8228..9082)
                     /locus_tag="AC1PIW_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbL"
                     /protein_id="HIP6605608.1"
                     /translation="MIKNVTSPFVFLASEKVSSDSLFEGFQVDLESTANLVKSLADFN
                     PTVWSYMTAITKGIMQPLGVAILAVVLVLEFSKMAKKIANSGGAMTFEAMAPMIVSYI
                     MVAVVITNTTVIVEAIIALASYVIEQVAGLVTNGGANYDTITGIKGSGIVGKMIIGFF
                     AILIWLVRMVSIMVVNILITIRFIQLYLMIPFAPVTIPTFLSDDWRSVGIGYLKNIMV
                     YAVQGILIFLIVSLVPLFESAGKIAVSNGAGVMESLAIMFGGLVQAILLIIALVGSQR
                     TARSILGM"
     gene            complement(9101..9343)
                     /locus_tag="AC1PIW_000652"
     CDS             complement(9101..9343)
                     /locus_tag="AC1PIW_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605609.1"
                     /translation="MNQKLTGFVYGVDASSMFSQAMSLLQKGLIAVGAFLVVMGIINL
                     STNIKDGGPGVRNAILEIVGGVMVGAAGAFVTQITI"
     gene            complement(9361..11178)
                     /locus_tag="AC1PIW_000653"
     CDS             complement(9361..11178)
                     /locus_tag="AC1PIW_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HIP6605610.1"
                     /translation="MYSRQKAFVFGLLGLAFGYFCHRLTLLYDSLTNAPPMDRLAYIL
                     GEGLNQVFNPLWLFTFTQKSLLAFILGILTMMLVYLYVSTGQKVYREGEEYGSARFGT
                     SKEKRYFYSKNPFNDTILARDVRLTLLEKKKPQFDRNKNLIVIGGSGAGKTFRFVKPN
                     LIQLNCSNIVVDPKDHLAEKTGKLFLENSYQVKVLDLVNMTNSDGFNPFRYVETENDL
                     NRMLTVYFNNTKGNGSRSDPFWDEASMTLVRAIASYLVDFYNPPGSSKQEQEARRKRG
                     RYPAFSEIGKLIKLLSKGDNQDKSVLEVLFEDYAKKYGHENFTMRNWADFQNYKDKTL
                     DSVIAVTTAKFALFNIQSVIDLTQRDTMDLKTWGTQKTMVYLVIPDNDTTFRFLSALF
                     FSTVFSTLTRQADVDFKGQLPIHVRSYLDEFANVGEIPDFAEQTSTARSRNMSLVPIL
                     QNIAQLQGLYKEKEAWKTILGNCDSLLYLGGNNEETFKFMSGLLGKQTIDVRSTSRSF
                     GQTGSSSTSHQKIARDLMTADEVGNMKRDECLVRIAGVPVFRTKKYFPLKHKYWKLLA
                     DKETDDRWWNYHINPLAKEEELDLSDYQIRDLSTETSLH"
     gene            complement(11178..11666)
                     /locus_tag="AC1PIW_000654"
     CDS             complement(11178..11666)
                     /locus_tag="AC1PIW_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605611.1"
                     /translation="MSSEQQERQAMQYVERSSLLTVRTLLKLLEWSARQALAQDSAYK
                     IGVQKLEELLQSPYAIEAIDVSKDVLDKPIDVEKFKELMESEKLPIAISWQKDYLYFY
                     AKDKTLLDHHLDELLKKLMSNPEKLEGLTFDKTLDQEIELAKEKIRVTEPSAVKTKEV
                     TL"
     gene            complement(11743..12327)
                     /locus_tag="AC1PIW_000655"
     CDS             complement(11743..12327)
                     /locus_tag="AC1PIW_000655"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIP6605612.1"
                     /translation="MIRIVLFYLAIQLNGLLVSLYLKEYLTIEGIVLLQLVFLSVTCF
                     EIARHKTVQEKNMTWQNHLRWLLLGFMCMVTFAVFISFLFPVQTRNQELLVEVGKQVP
                     HLIFLLFLVNASVLEEIVYRQLLWEKLTFPFVQIGVTSFLFVLSHGPNQLGSWLIYSC
                     LGLTLAVVRLKTDCMTAIALHLLWNSLVYVLTFL"
     gene            complement(12330..12563)
                     /locus_tag="AC1PIW_000656"
     CDS             complement(12330..12563)
                     /locus_tag="AC1PIW_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HIP6605613.1"
                     /translation="MNERFWDNLEIILAEKDLTWAELARKVFKGQYVYPSEFNRLYQK
                     LRHYKSNRLMPQIRWVERIVLVLDIDYEDLFKR"
     gene            complement(12572..12955)
                     /locus_tag="AC1PIW_000657"
     CDS             complement(12572..12955)
                     /locus_tag="AC1PIW_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605614.1"
                     /translation="MLEVYLGNNADTNQDLLTILTTYGVTYRCTKACDVNREIILSLF
                     AKTTDCFELLSPRFLRFKRQYTISLNELVQLVLQKPDQNLRLPLIVCQNHVYPAIGLD
                     EVRTFLPRQVKEDLFQATLMKQVTG"
     gene            complement(12966..13394)
                     /locus_tag="AC1PIW_000658"
     CDS             complement(12966..13394)
                     /locus_tag="AC1PIW_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605615.1"
                     /translation="MNLLHKKSILECTELEERIHQVETNQLLQKILSLPNFDCDFEVT
                     FEDDYHKEMNDPLFYESNLHRIFDFMETRDIKNGVDTLLTKDNHLAFRAFGENYTARG
                     KDGILTTLVTVKCFGEGRMPIDMSRYFSTPEPTVDNSLTL"
     gene            complement(13378..14733)
                     /gene="dcm"
                     /locus_tag="AC1PIW_000659"
     CDS             complement(13378..14733)
                     /gene="dcm"
                     /locus_tag="AC1PIW_000659"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HIP6605616.1"
                     /translation="MKFLDLFAGIGGFRLGMEAQGHECLGFCEIDKFARTSYKAMFNT
                     EGEIEYHDIKEVTDHDFRQFRGQVEVICGGFPCQAFSLAGRRLGFEDTRGTLFFEIAR
                     AAKQIQPRFLFLENVKGLLNHDKGRTFATILSTLDELGYDVEWQVLNSKDFQVPQNRE
                     RVFIIGHSRRYRSRFLFPLKRENSPAHLERLGNINPSKRGLNGEVYLTTGLAPTLTRG
                     KGEGAKIAIPVLTPDSLEKRQHGRRFKENLDPMFTLTSQDRHGVVVAGNLLTSFDQTG
                     RVFDISGLSPTLTTMQGGDKVPKILLREELPFLKIKEATKTGYAKATLGDSVNLAYPD
                     STKRRGRVGKGISNTLTTSDNMGVVVAALEYRQDKWYEVTGIVLEGKLYRLRIRRLTP
                     RECFRLQGFPDWAYERAESVSSKSQLYKQVGNSVTVTVIEAIAREFRRIEEEEKHEPT
                     T"
     gene            complement(14882..15700)
                     /locus_tag="AC1PIW_000660"
     CDS             complement(14882..15700)
                     /locus_tag="AC1PIW_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HIP6605617.1"
                     /translation="MKRITANQYQTSERYYKLPKVLFESKRYKDMKLEVKVAYAVLKD
                     RLELSLSKGWIDEDGAIYLIYSNSNLMALLGCSKSKLLSIKKTLRDYGLIDEVQQSSS
                     EKGRMANKIYLGELEHETTPVLHTDGASVKKTLGGSQRKTGPVLNSAPSETEGSETKY
                     SETKGSDFLIEDEEERQLVDEKQEENFTSKVDGVTKYDRDYIWGLVYDQLRQTGLSQS
                     ASDYAMIYFSNRYQYALEHMRFARSAEVIAEYVFNGVLSEWTKQLRRQEVKGGD"
     gene            complement(15703..15870)
                     /locus_tag="AC1PIW_000661"
     CDS             complement(15703..15870)
                     /locus_tag="AC1PIW_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605618.1"
                     /translation="MKKKEQSSRQIVMCHLVTILGVDIVKATHLVDELEQLGLIRFDE
                     FGNVGLLVLEG"
     gene            complement(16081..17442)
                     /gene="rlmD"
                     /locus_tag="AC1PIW_000662"
     CDS             complement(16081..17442)
                     /gene="rlmD"
                     /locus_tag="AC1PIW_000662"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIP6605619.1"
                     /translation="MLKKNDIVEVEVVDLTHEGQGVAKIDGFVFFVDNALPGEKISMR
                     ILKLKKNIGFGKVEQWQSFSQDRNQDLDFTYLRTGIADLGHLTYPAQLAFKKKQVENS
                     LRKIAGISEIAVADTLGMDSPLAYRNKAAVPVRRVNGQLETGFFRKNSHDLVPIEDFY
                     IQHKEIDALVLATRDLLRKLDLKPYDEKEQTGLVRNIVVRRGHYSGQLMLVLVTTRPK
                     IFRVETLIERLTSQFPNLVSIVQNINDQNTNAIFGQEFRLLHGSETIADTMLGNEFEI
                     SAPSFYQVNTEMAEKLYQTAIDFAELSADDVVIDAYSGIGTIGLSFAKQVKHVYGVEV
                     VEKAVLDSQKNAARNGIDNVTYVCDSAESAMQKWVADGVKPTVIFVDPPRKGLTESFI
                     KASTSVKPEKIVYISCNVATMARDIKLYEELGYKLTKVQPADLFPNTHHVECVVLLQR
                     SNG"
     gene            complement(17508..18833)
                     /locus_tag="AC1PIW_000663"
     CDS             complement(17508..18833)
                     /locus_tag="AC1PIW_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIP6605620.1"
                     /translation="MNKNNNILTHHEQLRLDYLQKNIHYLNDKESRELDYLLYKKELR
                     ERHGSPRQSLRSFTPTAYEDDYFEEDYFEEDDEQEELLPRYPERKSRKTRQAKPQRFQ
                     SPRIGPKAKVPKPKKARKKGGFKRFIGFVLLAIALVIVAMGYSFFKGMNSVSEKPVAE
                     VFNGVDTSNGTNILILGTDGRVGESSGETRTDTIMVLNVNNTDNKVKLVSFMRDTLVD
                     IEGYEYKLNTAYTLGEQDNQQGAEEVRKALKNSFDIDIKYYAMVDFATFATTIDTLFP
                     EGVTIDAQFSTINGEVVSSVEVPNDLQLEENAPAYQTIQVGVQQMDGKTLLNYARYRS
                     DDEGDFGRTRRQQEVLSAVMTQAKNPMKLFSGSEAIGKVVAMTPTTVPQSFMWLQGPG
                     LLLDAANGIERVTIPENGDWIDDYDMYGGMALRVDFEKYQQRLAELGLR"
     gene            complement(18845..19672)
                     /gene="pheA"
                     /locus_tag="AC1PIW_000664"
     CDS             complement(18845..19672)
                     /gene="pheA"
                     /locus_tag="AC1PIW_000664"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HIP6605621.1"
                     /translation="MQVAYLGPRGSFTHQVAQQVFPTADLQAFETITEVIKAYETGEV
                     TYSVIPVENSIEGSVHETIDYLFHQAEIHAVAEIVQPIAQQLLATSADKAVEVIYSHP
                     QAIAQGKKYIQEHFPQARIEITVSTAYAARFVAEHPEQPFAAIAPQAAAVEYGLEVIA
                     QDIQEISENFTRFWILGDKAPSIALEQVAKKVSLALTLPDNLPGALYKAMSVFSWRGI
                     SLTKIESRPLKTALGEYFFILDLDNQSEELLTYSQKELTSLDITYKVLGNYLVYMTS"
     gene            complement(19663..20154)
                     /locus_tag="AC1PIW_000665"
     CDS             complement(19663..20154)
                     /locus_tag="AC1PIW_000665"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIP6605622.1"
                     /translation="MPIVLLGFMGVGKTTTAHLLNLPVYDMDHIIEERIGMPIADYFS
                     LEGEASFRQLETEVLKGLLDLPSNCIVSTGGGVIKSEVNRELLLANREDNVLLTASFE
                     VSYQRIRKDRQSQRPLFLQCSKEEFEALYRERMALYQGLADTVIDTDKLIPEQVARKI
                     LCK"
     gene            complement(20163..21443)
                     /gene="aroA"
                     /locus_tag="AC1PIW_000666"
     CDS             complement(20163..21443)
                     /gene="aroA"
                     /locus_tag="AC1PIW_000666"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIP6605623.1"
                     /translation="MKLRRSVEKLKGTIRVPGDKSISHRSIIFGSLAKGVTRFHNILR
                     GEDVLSTMQVFRDLGVKIEDNGDIVEVHGVGFDGLQAPKNDLDMGNSGTSIRLISGVL
                     AGQDFEATMFGDDSLSKRPMDRVTIPLSQMGVEISGQTERDLPPLTIKGNKNLKPIRY
                     QLPVASAQVKSALIFAALQAEGESVIVEKELTRNHTEDMIVQFGGQLEVNGKEIRIQG
                     GQEFIAQEITVPGDISSAAFWLVAGLIIPGSKIVLENVGINETRTGILDVIKAMGGKM
                     TLSNIDELAKSATITVETSELKATEIAGELIPRLIDELPIITLLATQAHGTTIIRDAE
                     ELKVKETDRIQVVADALNSMGATIEPTEDGMIIHGPTALHGAEINTFGDHRIGMMTAI
                     AALLAKDGEVVLERAEAINTSYPAFFEHLNSLMD"
BASE COUNT         6207 a         4745 c         3869 g         6666 t
ORIGIN      
        1 aatgactact gtacctactc ctttgggaaa caacgtaagg gtatatgatt ttgaacgtat
       61 tatttgtgat tttgtaatac atcgagaaaa gattgattcc gaactctttg tcaagacact
      121 tcaatcttat gggaactatc ctaaaaaaaa tctagcaaaa ctctatgaat atgcgacaaa
      181 aatgaatact ttagaaaaag ttaaacaaac tctggaggtt ctaatatgaa caaagctaaa
      241 ttaacagcac tctgtcataa aatatcaaag aatactggtt taacctttaa ttccgtcatg
      301 acctattact tcctagaagt cattttgaaa aaattaagtc aaagttctta ttcaaatcac
      361 tatatcttca aaggaggatt cctactttcg aatgtcatcg gagttgaatc tcgtagcact
      421 gttgatattg actttttatt ccatcaaata acactctcag aagaaactgt taaacagcaa
      481 ctcaaggaaa ttttagcaga ttccgaagaa ggtatatctt ttgtgattca atcaatcaca
      541 gctattaaag aaagtgatga ctatggtggc tatcgagcaa caatttcgtg ccaacttgag
      601 aatattaaac aagttatcca tttggatatt gcgacaggtg atgttgtaac tcctcagccg
      661 attacatacg actataaagc tatttttgat gaagacaatt ttccaatcat tgcttataca
      721 attgaaacaa ttctagctga aaaacttcaa accatctatt cacgtaactt cttgaacagt
      781 agaagcaaag atttttacga tgtttatatt ctttccaaac tgaagaaaga agacattgac
      841 ttcattcagt tgagaaatgc ctgtcagaga acattttcat atcgcgaaac agaactcgat
      901 tttgtaaaga ttattgaact actcgagagg ttcaaatctg accctattca gaataaacaa
      961 tggcaaaatt attcaaaaaa atatagctat acaaaaggga tttcacttgc agatgttctt
     1021 gatgagatga ttagtcttat tacaattatt atttctactg actctgatta aaacttttaa
     1081 ctgtcaaact aggtaatcgt gacattaaac gaaaaggaca tgctaacatt tagttatttt
     1141 gttagcatat ccttttttat ttcgtcgtat acgcaaaact caagctacta tccactccag
     1201 ataatttacg gaaggtgtag ttgggattgc cattgtagtt ggtttcggag ataaggaatg
     1261 agccatctgg gtagactttc tccacaaaag ccacatgtcc gtattctgtt ggtgttccat
     1321 gtcctcctcc cgcaaaggaa aggatagctc ctgcttgtgg acttgtccct gtctctccac
     1381 cgagacttgc ggctgtacgc acccaatctt ggccattgcc catggtactg ataatcggca
     1441 gcacaaccga taacctccga agaatactta aaaataaaaa aaagaggagg actgacgcat
     1501 cagaccctcc cagacgtgac tagcacgccc caattcgata caactatgat accactaatt
     1561 tcgtttttcg tcaataaatt tacttaattg ttgaattgat gtttttctat ttgtactgat
     1621 aatcttaaag agaatatcac gattattgag tgaaacatca aatcgttcaa caatttcatt
     1681 gaattgattg atgtagttat tgaggactac tgtatcaaat tttagaatgt tcaaagtcca
     1741 aattagtaga atgaaataat cttcaatgaa aacacaagtg aatgtcgata atcctaaatc
     1801 ttgttttatc tgttttttga gtgctttaga tacaccgctt gaaaaattag catcaaaaat
     1861 cgcactgttg tgcatggtag cattccttaa ctcaataaga gcgttgacaa ctaactcgag
     1921 agagtttgac ctatttggaa taatcgtgaa atcttgattc aatttttcac gacttgactt
     1981 agttaaactt cctaaaaatc tactaaactg tcctaaagta ataacttcaa aataagccca
     2041 gagaggaatt tgataatcac tttttaaata atgcttaatg acctcatttt tatctgagta
     2101 atagtcaatt gtatccagta cattttgtct agtttcatta tactgatcga gtttattacc
     2161 atatttttca tggtcttcag gatagtccat gtaacaagct aattgatgtt ctaatatatc
     2221 ttctatccca ctactagatt ctgcgactat tgaatcgata gtccgatttt ttaatgctgt
     2281 ctctacaagc attactattg gataaaatag tccttttaac tccatatcaa aattgtagat
     2341 tgcttcaatt tccttaaatg aagaaagggg cagaatccca tctgaattgg attttttaac
     2401 gaatctatat cgtttataac catgataata tcccatattt aagagtgtga cagagtcacc
     2461 atcttgaata tctattttat gatttttaaa gatatggtta actagatccc cagaagttaa
     2521 accagttttc gactcttttt tcattttttc aataattgtt tccattaaaa tattcagtaa
     2581 cctctcctta gtattatatc atatcactaa acttatcaaa gtaactaaaa ggtagaattt
     2641 cttacgaaat atttgcaaaa caatatgcta acaaatccaa ttattgttag catatccctt
     2701 tttatttcgt cgtatacgca aaactcaagc tactatccac tccagataat ttacggaagg
     2761 tgtagtttgg attgccatta tagttggttt cggagataag gaatgagcca tctgggtaga
     2821 ctttctccac aaaagccaca tgaccgtatt ctgttggtgt gccatgtcct cctcccgcaa
     2881 aagaaagaat agctcctgct tgtggacttg tccctgtctc tccaccgaga cttgaggctg
     2941 ttcgtaccca atcttggcca ttacccatgg tactgataat aggaatcttc tcaccatttt
     3001 tacctttgag ttttagacct agttggttga tacgggctgc gactccccaa gtacattggc
     3061 cgtaggcata ccccataccg tcaccacctc ctggaacaga gttttcaaaa aggtctcctc
     3121 gtacagcttc aagtgctttt ggatcacttt gtgctgtccc accgttgggt tgactaaagc
     3181 ctttttcaat ttggtaatac cactcacttg ctcttgtctg acgttcaagt agcttatcac
     3241 cactatttcc ttcccagtaa atgagaaaga gttgggcaag actagctgga ctacctgaat
     3301 ttttgaagaa gtcctttaac cagttggtgt aataaggact atccccatgc aacatgaaat
     3361 ccagttgtaa ccctaagtca taccactttt gatgttttcc tttggcatat tcgagcaata
     3421 aggtatgtct gcgtgaccca tccgcggtat ctgtccattg gcctaagcct aaaccacgtt
     3481 tgagaatatt tgggtaacgt ccattgtata tagttggacc attaagtgag agccagccct
     3541 catcatccca cgaactgtct gtcgcaccaa tagggggaga tagatagtcg ccctcggcac
     3601 gtttcgggtt gatggaggat tctaccgacc agttacctag aatggctgcg atggcttgat
     3661 tggtcgcacc tttgctttta agatagtcat aaattgcctt ggctctctcg aactcatcgc
     3721 cgccaaactg accgatagtt ggaaggatgg ttgtctgaac ctggattact tttggaaagt
     3781 agaaagctgg attgacattg accaacttat ccgtatcatc atcaaccttt tgataggtga
     3841 cttttagacc cgttccgtct ttggtctgac caataatatc tcctgccaag acatgttgat
     3901 gttcactcac tcggcctgaa tgaatgccaa acaaggttag tttagtctta ttcactccct
     3961 taccagatgt caaaacaacg ttctctccat caagagatac cttgccatcc atcggagcta
     4021 caatgacttg accttctttt gcttctaaga tgatatggtg atgaagcgtt ggtttctcat
     4081 caatgacctc ataaccatat cggtaggtca tgtttaagct atcctcatcg gtctgtccct
     4141 gaaagggatt gtccaattct tgaagggcta aatagttgcc ttcttcggtc aactccttta
     4201 gttcttcttg gtcctcatca gacagcttgt aagcaggttc cttatacaag tcagacatgg
     4261 atttaataga atcaccacca tttaagtccg tccacacttg actgagaaat gctttgtagg
     4321 tcgtaccacc cgtttccatg aaatcgtcga gcttgtagtc ttggtattga tgattcatgt
     4381 aaaccataac ctcatcaatc ttagtgtaaa aggtaattcc cttttcgttg gttcttgtat
     4441 gttccgcatc ttccaacgtc atgtgggtat aactcttact caattctatt tcatcttgtt
     4501 gaatgacact actacctgaa acactcatga aaaagctcat catcaaaagg agtatcaaga
     4561 gaatgcctga gataatccaa gtcaaaggat ttcccgcaag gacagaaaag aaagtcatac
     4621 ttcccttaat ggcttggacg attcctttga aacctgcgac actttgtttt ctggcaagct
     4681 ttaaaaaggt ttggtagcgt cttcttggtt taagaggttt ctggcgtgtg aatccttttc
     4741 cctttttgaa gttctggaat cgttcctttc catgagaaaa ttttgttttg gtaaaccgca
     4801 caccagtctg tccacttttt actgccacct tacctgcttg ataggtaaac ctgccatagc
     4861 gtctgacttt caggctagta tcctttaggg ttctaagtcc atctaaatca tcctcctgtg
     4921 ctatcaggtc aagggattca gatgcagcta agcctaatcc agctctgact ttggtagaac
     4981 ttttcttaga cttttggact ttcttgagtt gtttgacttc acgtttggca gaactcactt
     5041 ctttttctgc actgagtcga tcaaatgatt tttcttggtg aaactgaaag cgagacttca
     5101 gggaaggatt ttctttttgg aaagtaaact tggaatcaac tgcctgttgt ttctcactta
     5161 gtttcacttc tgacaaatgt tttcttgccg tccgcaaacg ttcttgggca tgtggaagtc
     5221 gatagttctt gatttcctta accttcaaca atctaggaga cacataggta atttccttta
     5281 ggaacttctc ttctgcttcc ttttggctac tgagtagatt ttcttttaac tctgttttct
     5341 tttcctgaaa aagagaattt tgggctggtt tacgaaatcg tcctttttta ggaacagtct
     5401 gtcttaaggt cctaacttct ttccgataat gcatacgggc tgattttaag ttgcttcgaa
     5461 agtttttcct ggcctgtttg actacctttt tatcctcttt catctcatgt cctcattttt
     5521 tcagggtctg tactcatgat gtcaaagagc ttggtgtgtt gtggaatctt attcttaaac
     5581 gggacaacgg tggaaccggc cttaattaag cctgcccctt tttcaggatt gaccaagtat
     5641 ttctccagtt ccttggaaag tccaagcata tgcaccaatt cttcgcgatc gctcttagcc
     5701 tgttttaata gaatcataaa ctcgctattg gcaataatac gtctgccatt ggcatccaaa
     5761 agaagtgtct ccacattttg agtaatgcca gttggtatcg ccccatactt acggacacga
     5821 ctccagagtt taaagaagaa atcagaggca tatttgtcca ataaaagaag ctgcatttca
     5881 tcaaagtaaa tccaggtctt cttccctagt ttttgatttt tcaccacacg attccaaatc
     5941 tggtcaaaga taaccatgag ggcaatctgt tttaattcat cgcctaactt tttgacattg
     6001 taaatcagga aatgactgtc tgttttaata ttggtccgat gtgaaaatat gtccagcgac
     6061 ccttccacat agagttccat atccagtgcc aagtccttag cttcctgttc aggttgttga
     6121 gctaagacaa agacccattc gaccaaggac ggtgtgtcaa aatgcttata ggtgagtcgt
     6181 gtcacacggt caatcaagga tttttcacga ccatccattt tacggtccaa gagctttcca
     6241 atccaagaca agagaaattc ggacttgacc ttaaccggat cctcatccat attctcatct
     6301 gatagatcta atacatttaa gaaggtagtg gaatctgggg caatatcaat actctccccg
     6361 ccaaaggctt gaccgataat actatactca ttttctgggt caacaataat aatttcagta
     6421 tcactatctg cttccttgag tttagtagtg atgatttcat gttttgtcgc catccctttc
     6481 cccgctccag aagtccctaa aatcaaacct gacggagtat ttaacttgcc acggtcaata
     6541 ctgatgatat tacttgagat ttgattgatg ccataaaatt tcccaccctt gtcctgaata
     6601 tcaacggaag tccaaggggc attcacggca atgtttgaag tcaatagaga acgagaaact
     6661 ccttcgagat agttcttccc aaatggcaag agactattaa aagcagcttc ttgcatgtag
     6721 gtcaagttgt caataatcat atcatttgac cccgccactt gcttgataat atcaagagat
     6781 tgtttgagtt ggtcttcagt atctgcaagt acaccaatta gaaatacggt gtcaaacagc
     6841 ttatctcctg tttgggtcat ggtttgaaga agagcttctg cttcatcgat gttgttttca
     6901 agaacatggc cgaccttctc caaatagatt ccagtccgag ccatcttttg ttgttcccca
     6961 atcttttgtg attccatcaa ggtcttcttg gttcgcagct tgatcatggt ttcagacttg
     7021 gtagagcctt tagcatgtag gctaatcatc acttccaaat ctgactgcat gaggtcacga
     7081 ataaatttat cccctagctc cataccatag tcacgaacat agacaatctg taataatcta
     7141 tcgtccaatt caatatgatt cttgtgttta aaggaaaggt aggttggagc aataaagtgt
     7201 ttggtggatt gacctgacag ggttaaatcc ttgtaagaga atggtaaatg attttcacct
     7261 cgtaacatat ccgcaaggac attcactcgc tcctctccac caagcagacc aagagacaca
     7321 tcaatctcag aaaagccact cttgaaatat tccccaatct gtgacagaga acgaaaagct
     7381 agttttggtg tttggtcgct cttgccaaat gaaagaaact tgacagccga aaagttgttc
     7441 tcacccgcct ctaagttgga atccatcatg cgattcaatt catcacgata agcatcaaac
     7501 ccatcttcct gcaagggata gagaatactc ttacggaatt tttccagatt gaccttttga
     7561 ttgaaaatgg tcagttggaa attggtctta tcatccagcg aattgattaa atcagaatat
     7621 ttctccacaa tagctccttt gtcatctaag cctaccgttt gataattgac atcacctaaa
     7681 agataggttt gtgagaaata gcttggactg acttgcataa gtccattctg aaaaagccct
     7741 tgataggcta acgtatttac ggtagaaggt ctaatctcct gcttctgtgt tttcgtcttt
     7801 tgctttttgt cattagaaga ggtctttggt ttcattgagt gttttaattt tttcacgttg
     7861 gtctcctttt cttccagtaa atgtgcggtc tggcaccgtt agttcataat gaaaacagta
     7921 cttgaggtat atttcaaatg gtaaatcgtt ggggcgatag accccaaata acatgagtgg
     7981 aatggtaaag ctaaaaacaa aaccatagac aaaccaatcc ccaaattgcc agtaaaatag
     8041 gttcaatcct aataccaaaa tggttatggc gatggcagga aatacaaaaa taacctgtct
     8101 agttgtaaat cccagccatg ccctgtgttg aacctttgag atgtccttaa agacacgagt
     8161 gttcataaat gttctccaat ctaagtaagc tataaaaaga ggaggcgaga taatgattcc
     8221 cttcctccta catgcccaaa atacttcggg cggttcgttg actgccaact aaggcaatga
     8281 ttaacaagat ggcctgtacc aagccaccaa acataatggc gagtgattcc ataactccag
     8341 ctccatttga tacggcaatc tttccagccg actcaaacaa gggaacaagc gatacaatta
     8401 gaaaaatcaa aataccttga acggcataga ccatgatgtt cttaaggtag ccaatcccaa
     8461 cacttctcca gtcatcacta aggaaggtcg gaatagtaac aggggcaaaa ggtatcatca
     8521 gatagagttg gataaagcga atggtaatca agatattgac aaccatgata ctcaccattc
     8581 tcaccaacca aatgagaatc gcaaaaaagc caataatcat ttttcctaca attccagatc
     8641 ccttgatgcc tgtgatggta tcgtaattag caccaccatt tgtgacaagc cctgccactt
     8701 gttcaataac ataagaggca agagcgataa tggcttccac aataaccgtc gtattggtaa
     8761 tcacgaccgc gaccataatg tagctaacaa tcataggtgc catggcttca aaggtcatcg
     8821 caccacctga gtttgcgatt ttcttggcca tttttgagaa ttcgagtacg agaacaaccg
     8881 caagaatagc tactcccaag ggctgcataa tccccttggt aatggctgtc atgtaagacc
     8941 acaccgtcgg attaaagtcc gctagtgatt taaccaagtt agcggttgat tctaaatcga
     9001 cctgaaaccc ttcaaaaagg ctatcgcttg acactttttc agaggctaga aatacaaaag
     9061 gtgacgttac attctttatc atgtcatatc ctgacctcct ctaaatggta atctgtgtca
     9121 caaaagcacc tgcggctcct accatgaccc caccaacaat ttctagaatg gcatttcgga
     9181 cacctggccc accatcttta atgttggttg aaaggttgat aatgcccata acaacaagaa
     9241 aggctcctac tgcaattaag cctttttgta atagagacat ggcttgggag aacatggagc
     9301 tggcgtccac tccgtataca aagcctgtta gtttttgatt catatttttc ctcatttcta
     9361 ttaatgtagt gatgtttcag tgcttaaatc ccttatttga tagtctgaca agtccaattc
     9421 ttcctcttta gccaagggat tgatgtgata gttccaccaa cggtcatcgg tttccttatc
     9481 agcgagcaac ttccaatatt tgtgtttgag tgggaaatat ttcttggtcc gaaaaacagg
     9541 aactcctgca atgcgtacga gacattcatc tcgtttcata tttcctactt catctgctgt
     9601 catcaagtca cgggcaattt tctggtgaga ggtagaactt gagccagtct gaccaaatga
     9661 acgactggta cttctgacat cgatagtttg tttgcctaga agtccactca tgaacttaaa
     9721 ggtttcctca ttattcccac ccaaatagag gagactgtca cagttcccca aaatggtttt
     9781 ccaagcttct ttttccttat aaagtccttg gagttgggca atattttgca agattggaac
     9841 tagactcata ttcctagagc gagctgttga ggtttgttcg gcaaagtctg ggatttctcc
     9901 gacattcgca aactcatcca gatagcttct aacatgaatc ggcagttgcc ctttaaagtc
     9961 aacatcagcc tgtctggtca aagtggagaa aaccgtagag aaaaataaag cagataggaa
    10021 acgaaaggtc gtatcattgt ctggaataac aagatagacc atggtctttt gagtgcccca
    10081 cgttttcaaa tccatagtgt ctctttgcgt caaatcaatc actgattgga tattaaaaag
    10141 ggcaaattta gctgttgtga ccgcaatcac cgaatccaag gtcttatctt tatagttttg
    10201 gaaatccgcc caatttcgca tagtaaagtt ttcgtgaccg tattttttag cataatcttc
    10261 aaacaagact tcaagaacac ttttgtcctg attgtctccc tttgataaga gtttgatgag
    10321 tttcccaatc tctgaaaagg ctggataacg gccgcgctta cgtcttgctt cctgctcttg
    10381 cttggaactt cctggaggat tgtaaaaatc caccaaataa gaggcaatag ctctcaccaa
    10441 tgtcatggaa gcctcgtccc aaaagggatc actgcgagaa ccatttccct tggtattgtt
    10501 aaaatagacc gttaacatgc ggttcaaatc attctctgtt tctacataac gaaaaggatt
    10561 aaaaccgtct gaattggtca tattaactag atctaaaacc ttgacctgat aactgttttc
    10621 taaaaagagt ttacctgtct tttcagccaa atggtcttta ggatctacga caatattgga
    10681 acagttaagt tggataaggt tgggtttaac aaaacgaaag gttttacctg caccagaacc
    10741 cccaatgaca atcaaatttt tatttcggtc aaactgtggc ttcttctttt ccaacaaggt
    10801 tagacgaaca tcacgagcta aaatcgtgtc attgaaagga ttcttactgt aaaagtatcg
    10861 cttttcctta ctggttccaa atcgggcaga accgtattct tccccttctc gatagacctt
    10921 ctgtcctgtc gatacataaa gatagactaa catcatcgtt agaatcccaa ggataaaggc
    10981 aagaagagat ttttgagtaa aagtaaaaag ccataaagga ttgaagactt gatttagccc
    11041 ttctcctaag atgtaggcta aacgatccat aggtggggca ttggttaaac tatcatagag
    11101 tagggttaga cggtgacaga aatagccaaa agcaagaccc aagagtccaa agacaaatgc
    11161 tttttgccta gaatacatta caaggtcacc tccttggttt tgactgccga aggttctgtc
    11221 actctaattt tctctttggc cagttctatc tcttggtcca aggttttatc aaaggttaaa
    11281 ccttctagct tctctggatt agacatcagt ttcttcaaca attcatctaa gtgatgatca
    11341 agcaaggtct tatccttggc ataaaagtaa agataatcct tttgccaact aatagcgatt
    11401 ggcagttttt ctgactccat cagctcctta aatttctcca catcaattgg cttatctagg
    11461 acgtctttac taacatcaat cgcttcaatg gcgtaagggc tttgaaggag ttcttctaat
    11521 ttttgaaccc caatcttata cgctgaatct tgagctaagg cttgcctagc cgaccattct
    11581 aagagcttta agagggttct gactgttaat aaactacttc tttccacgta ttgcatcgct
    11641 tgacgttctt gttgttcaga tgacatggtg acctcctctt ctttcaattc cctatcaaac
    11701 ataaggtgct tccataatac ggaagcactc ttgattttgg tatcacaaga aggtcaagac
    11761 ataaaccaaa ctattccaaa gtaaatgtaa ggcgattgcc gtcatacaat ctgttttcaa
    11821 tcgaacaaca gccaaggtca acccaagaca gctatagatg agccaactcc ctaactgatt
    11881 ggggccatgg gatagaacaa agagaaaact ggtcactcct atttgtacaa aagggaatgt
    11941 caatttttcc cacagcaatt gcctataaac aatctcttca aggacacttg cattgaccaa
    12001 aaacaataaa aagataagat gaggaacctg ctttccaact tctaccaata actcttgatt
    12061 cctagtctga actggaaata gaaaactgat gaagactgca aaagtcacca tacacataaa
    12121 accgagaagc aaccatctta ggtgattttg ccaggtcata tttttttctt gaactgtttt
    12181 atgacgggca atctcaaagc aagtcacact taaaaagacc aattgtagca ggactatccc
    12241 ctctattgtc agatactctt tcaggtataa actcacaaga agaccgttaa gctgtatggc
    12301 tagataaaac aacactatcc ttatcattgt cacctcttaa ataaatcttc ataatcaata
    12361 tctaatacca aaacaattcg ctcaacccat cttatctgtg gcatcagacg attggatttg
    12421 taatgtcgca atttttgata gaggcgatta aactcactgg gatagacata ttgacctttg
    12481 aatactttgc gagctagttc tgcccaagtg aggtcttttt ctgccagaat gatttctaaa
    12541 ttgtcccaaa atcgttcatt catcttcaca cctaccctgt cacttgtttc atcaaggtgg
    12601 cctgaaatag atcttctttt acctgtctgg gaagaaaggt tcgcacttca tctaacccaa
    12661 tagctggata gacatgattc tgacagacaa tgagaggcag acgaaggttc tggtctggct
    12721 tttggagaac gagctggacc agctcgttta aactgatggt gtattgacgc ttaaagcgaa
    12781 gaaatcgtgg cgacagcaac tcaaaacaat ccgtggtttt ggcaaagagt gacagtatga
    12841 tttcacggtt tacatcacat gcttttgtac aacggtaagt cacaccatag gtcgtcaaaa
    12901 tggttagtaa gtcttgattt gtatcggcat tatttcctag atatacctca agcataggct
    12961 cctccttata gggttaggct attgtcaact gttggttcag gggttgagaa atagcgactc
    13021 atatcaatgg gcatccgtcc ttcaccaaag cacttgaccg tcaccagagt cgttaaaatg
    13081 ccatcctttc ccctagcagt ataattttca ccaaaggcac gaaaggccag gtgattgtct
    13141 ttcgtcaata gtgtatctac accattttta atatccctag tttccataaa atcaaatatc
    13201 cgatgaagat tggattcgta gaatagggga tcattcatct ctttgtggta atcatcttca
    13261 aaagtcacct caaagtcaca atcaaaattg gggagcgaca atatcttttg cagtaactga
    13321 ttggtttcaa cttggtgaat acgttcttct aattcagtac actctaggat acttttctta
    13381 tgtagtaggt tcatgttttt cttcctcttc aattcttcta aattctctcg caatggcttc
    13441 aataactgtg acagtcacac tattgcctac ctgtttgtat agttggctct tactggaaac
    13501 actctctgct ctttcataag cccaatcagg aaaaccttga agtctgaaac actctcttgg
    13561 tgtcagtcgt cttattctca gacgataaag tttcccttct aagacaatgc ctgtgacttc
    13621 ataccactta tcctgtcgat attccagagc agcgaccacc actcccatat tgtctgaagt
    13681 cgtcaaggta ttggatattc cctttcccac gcgtccccta cgtttggttg agtctggata
    13741 agctagattg acagagtctc caagagttgc ctttgcgtac cctgtttttg tggcttcctt
    13801 gattttcaga aatggtagct cctctctcag caaaatcttt ggaaccttat ctccaccctg
    13861 catggtggtc aaggtcggtg acaagccaga aatgtcaaat actcttccag tctggtcaaa
    13921 gctagtcagc agatttcctg cgacgacaac accatgtctg tcttgactgg tcaatgtaaa
    13981 cattgggtct agattttctt taaagcgtct tccatgttgc cgtttttcaa gactatctgg
    14041 tgttaagact ggaatggcga tttttgcccc ctctccttta cctctcgtta gtgtaggagc
    14101 aagtccagtc gtcagataga cttcaccatt caaaccacgt ttagagggat tgatatttcc
    14161 tagcctttca agatgagctg ggctgttttc tcttttgaga ggaaatagga atctggaacg
    14221 gtatcttcta gaatgtccga taataaagac ccgttctctg ttttggggca cttggaagtc
    14281 cttactgtta agcacctgcc attcgacatc ataccccaat tcatccagcg tggagaggat
    14341 tgtggcgaac gtccgtccct tgtcgtgatt gagtaggcct ttgacgtttt ccaaaaatag
    14401 aaaacgtggt tggatttgtt tggccgctcg agcaatctca aaaaagagag tccctcgagt
    14461 atcttcaaat cccagtcgtc ttcctgcgag tgaaaaagct tggcaaggaa atcccccgca
    14521 gataacctcc acttgccctc taaattgtct aaagtcatgg tctgtgacct ccttaatgtc
    14581 atggtattct atttctcctt ctgtgttaaa catggcttta taggatgttc tagcgaattt
    14641 atcaatttca caaaagccca aacactcatg gccctgtgct tccattccta gcctaaaacc
    14701 gcctatccca gcaaataaat ctaaaaattt cattgttcct ctctttccaa ctaaaaaaga
    14761 gccttacact tgtaaaactc ttgcctatat atgttcatcc atttcaggtg cacggatgat
    14821 ttcaacaatc aaaatgatga tagaaatcag atagattccg cctagtatcc accaaatcat
    14881 cttaatcacc tccctttact tcttgtcgtc tcagttgctt ggtccattct gacagcactc
    14941 cattaaacac atattcagct atcacttctg ctgaccgagc aaatcgcata tgttccaaag
    15001 catactgata acggttacta aaataaatca tggcatagtc actagccgac tgagatagac
    15061 ctgtctgtct taactggtca taaaccaaac cccaaatata gtctcgatcg tacttggtca
    15121 cgccatcgac ttttgaagta aagttttctt cttgtttctc atctaccagc tgcctttcct
    15181 cctcgtcctc aataaggaaa tcactccctt tagtttcact atatttagtc tcacttcctt
    15241 cagtctcact aggggctgaa tttaagaccg gccccgtctt tctttgagac ccccctagtg
    15301 tttttttaac actagccccg tctgtatgta agactggggt agtttcatgt tctaattccc
    15361 ccaagtaaat tttatttgcc attcgacctt tttcactcga ggactgttgg acttcatcaa
    15421 ttaagccata gtcacgtaag gttttcttga tagagagtag ttttgacttt gaacagccta
    15481 aaagtgccat cagatttgaa ttggaataaa tcaaataaat agccccatct tcatcaatcc
    15541 aacctttact caaagacagc tccaacctat cttttaaaac cgcgtaggct accttaacct
    15601 ccagcttcat atccttataa cgtttactct caaacaagac ttttgggagt ttataataac
    15661 gctctgatgt ttgatattga ttagcggtaa tacgtttcat gattatccct ccaagactaa
    15721 aagtccaaca tttccgaatt catcaaatcg gattaaacct agttgttcca attcatcaac
    15781 cagatgagtt gctttcacaa tatcaactcc taagatagtc acgagatgac acatcacgat
    15841 ttggcgtgat gattgttcct tttttttcat gaattcctcc tgaaaataaa aaaacgagaa
    15901 caattagtaa aatgttctcg ctgaactatt atattttatg acgtatctat tttttgtcta
    15961 cgcagtcgac atccaaacag acattcacat caatgtactt tttttgaacc ttttttatgt
    16021 caaaatcact cttttcaata aagacaaaat gcttgaaaaa gtgcttaaaa taaaggattt
    16081 ttacccgttg cttcgttgta gcaacactac gcactccacg tgatgcgtat ttggaaataa
    16141 atcagccggc tgtaccttcg tcaatttgta ccccaattcc tcataaagtt taatatcacg
    16201 cgccatggtt gccacattac aagagatata gacgattttc tctggtttta cagaagtact
    16261 tgctttgata aaactttctg tcaaaccttt tcgtggtggg tcgacaaaaa tcacagttgg
    16321 tttgactcca tctgccaccc atttttgcat agcggattca gcgctatcac agacataagt
    16381 aacattatca atcccatttc gagcagcatt cttctgacta tcaaggacag ccttttcaac
    16441 gacttctaca ccgtaaacat gcttaacttg ttttgcaaag gagagaccaa ttgtcccaat
    16501 acctgaatag gcatcaatca cgacatcatc tgccgacaat tctgcaaaat caatggcggt
    16561 ctgatagagt ttttcagcca tttctgtgtt gacttgatag aaagacggtg cagaaatttc
    16621 aaattcattg cccaacatgg tatctgcaat agtttcactg ccatgcaaaa gacggaattc
    16681 ttgaccgaat atagcattgg tattctggtc attaatattc tgaacgatag aaacaagatt
    16741 tggaaattgg cttgtcaaac gctcaataag ggtttcaaca cggaaaatct tcggacgagt
    16801 cgtaactaaa actagcatca actgacctga ataatgacca cgacgaacaa caatatttct
    16861 cacaagtcct gtctgttctt tctcatcgta aggcttcaag tctaattttc tcaagaggtc
    16921 acgtgtcgct agaaccaagg catcaatttc cttgtgttga atgtaaaaat cctcaatcgg
    16981 aactaaatca tgtgaatttt ttcggaagaa tcctgtttct aactgcccat tcacacgacg
    17041 aactggaaca gctgccttat tacgataagc aagaggactg tccattccca acgtatctgc
    17101 tactgctatt tctgaaatgc ctgcaatctt acgtagacta ttctcaactt gttttttctt
    17161 aaaagctagt tgggctggat aagtgagatg ccccaagtca gcaatacctg ttcttagata
    17221 ggtaaaatcc aagtcctgat tacggtcttg tgagaagctc tgccattgct ccaccttacc
    17281 aaaaccaata tttttcttaa gttttaagat gcgcatggaa attttctcgc ctggaagagc
    17341 attgtctaca aagaagacaa aaccatcaat ttttgccaca ccctgcccct catgtgtcaa
    17401 atctacaact tctacttcga caatatcgtt tttctttaac ataattctac tttctatttt
    17461 tggaccacta aaaagtgacc gaagtcactt ctagtatacc atatttctta acgcaggcct
    17521 aactcagcca agcgctgctg gtatttttca aaatcaacac gaagagccat accaccatac
    17581 atatcatagt cgtcaatcca gtctccattt tctgggattg taacacgttc gataccattt
    17641 gccgcatcca atagaaggcc tggtccctgt agccacataa agctttgcgg tactgttgtc
    17701 ggagtcatgg ccacaacctt acctatagct tcggagccag agaatagttt cattggattt
    17761 ttagcctgtg tcataacagc agatagcact tcctgttgac gtctcgtacg accaaaatca
    17821 ccttcatcat ccgaacgata gcgcgcgtag ttaagcaagg tcttaccatc catctgctgt
    17881 acaccgacct gaatcgtttg ataagcagga gcattttctt ccagctgtaa atcattcggc
    17941 acttctactg agctaacgac ttcaccatta atagttgaaa actgtgcatc aatcgttacg
    18001 ccctcaggga aaagtgtatc aatcgttgtc gcaaaggttg caaaatccac catcgcatag
    18061 tacttgatat caatgtcaaa actattcttc aacgccttcc gaacttcttc agccccttgc
    18121 tgattatcct gttctcccaa agtataggca gtattgagtt tatattcata tccctcaata
    18181 tccaccaagg tatcccgcat aaagctaaca agtttgactt tgttatccgt gttattcaca
    18241 tttagaacca tgatggtatc cgttcgagtt tcacctgagc tttcgccaac acgaccgtct
    18301 gttcccaaaa ttaaaatatt tgtcccattc gatgtatcta cgccgttgaa gacctcagct
    18361 actggcttct cactcacgct attcatacct ttgaaaaaac tatagcccat cgctacaatc
    18421 actagcgcta tagcaagcaa tacgaaacca ataaagcgct tgaaaccacc ctttttacga
    18481 gcttttttag gtttaggtac tttcgctttg ggaccgatac gaggagactg aaaccgttga
    18541 ggtttggctt gtcttgtttt tctggattta cgttcaggat agcgaggcaa cagttcctct
    18601 tgctcatcat cctcttcaaa ataatcttcc tcgaagtagt catcttcata agccgttggt
    18661 gtgaagctac gtagagattg ccttggactt ccatgccgtt ctctcagctc ttttttatat
    18721 aatagataat ccaactcgcg actttctttg tcatttaagt agtggatatt tttttgcaaa
    18781 taatctaacc tcaattgttc atgatgggtt aggatattat tgtttttatt catctgtctt
    18841 catcctagct ggtcatatag accaggtaat tccctagtac tttataggtt atatctaaac
    18901 tagtcaattc tttttgagaa taggttaata attcctcaga ctgattgtca agatccagta
    18961 tgaaaaaata ctcacccaaa gccgttttca ggggtcggct ttctattttt gttaagctga
    19021 ttcctctcca tgagaaaaca gacatggcct tatacaaggc tcctggcagg ttatctggca
    19081 atgtcagtgc aagactgact tttttagcta cttgttccag ggcaatagac ggagccttat
    19141 cccccaaaat ccaaaagcgt gtaaaattct cgctgatttc ctggatgtct tgcgcaatta
    19201 cttccagtcc atattcaact gctgccgctt gcggagcaat tgccgcaaat ggttgttcag
    19261 ggtgttctgc cacaaagcga gcagcatagg ctgtactaac tgttatttca atacgagcct
    19321 gaggaaaatg ttcctgaata tatttcttcc cctgagcaat tgcctgcgga tgagaataga
    19381 taacctctac agccttatcg gcactcgtgg ctaacagttg ttgggcaatc ggttgaacaa
    19441 tctctgcaac cgcatgaatc tccgcctgat gaaagaggta gtcaatcgtt tcatgaacac
    19501 taccttcaat cgagttttct actggaataa cggaataggt aacttccccc gtttcgtagg
    19561 ccttgataac ctctgtaatc gtctcaaaag cctgcaaatc cgcagtcgga aagacctgct
    19621 gggccacttg atgtgtaaaa gaaccacgag gtcctagata ggctacttgc ataaaatttt
    19681 ccttgctact tgttctggaa tcagtttatc tgtatcgatt acagtatcgg ctaaaccttg
    19741 ataaagtgcc attcgttcac gatagagggc ttcaaactct tccttactac actgtaaaaa
    19801 gagaggtctt tgcgactgtc tatccttacg aattctctga taggaaactt caaaggaggc
    19861 agtaagaagt acattgtcct ccctatttgc caacagcaat tccctgttta cttcagattt
    19921 aatcacacca ccaccagttg atacgataca attactaggt aagtccagca atcctttcag
    19981 tacctctgtc tccagttgtc taaacgaagc ctctccttca agactgaaat agtcagcaat
    20041 aggcatacca atccgttctt caatgatatg gtccatatcg taaacgggga gatttaacaa
    20101 atgtgctgtt gttgtttttc caactcccat aaatccaagt aaaacgattg gcatacgttc
    20161 tcctaatcca tcaaactatt taagtgttca aagaaggcag gatagctagt attgatagct
    20221 tctgctcttt ccaatacaac ttctccatct ttggccaaca aggcggcaat ggcagtcatc
    20281 ataccgatac ggtggtcacc aaatgtattg atttcagcac catgtaaagc agttggtccg
    20341 tgaataatca taccatcttc tgttggttca atagttgctc ccatactatt tagagcatct
    20401 gcaacaacct gaatacgatc cgtttcttta actttcaact cctcagcatc acgaataatc
    20461 gttgttccat gtgcttgagt cgctagtaag gtaataatcg gcaactcatc aatcaaccga
    20521 ggaatcaatt cgcctgcaat ctccgtagcc ttcaattccg acgtttcaac tgtaatggta
    20581 gcagattttg caagttcatc tatgttagaa agagtcattt taccacccat agctttaatg
    20641 acatctaaaa taccagtccg agtttcattg attcccacat tttcaaggac aatttttgaa
    20701 cctggtatga ttaagccagc aaccaaccaa aaagcagcac ttgaaatatc tcctggaact
    20761 gtaatctctt gggcaataaa ctcctgacca ccttggatgc ggatttcctt gccattgact
    20821 tctaactgtc caccaaactg gacaatcata tcttctgtat ggtttcgagt caactctttt
    20881 tctacgatga ccgactctcc ctcagcctgc aaggcagcaa aaatcagagc tgattttact
    20941 tgagcagagg ctacagggag ttggtagcga attggtttta aattcttatt ccctttgatg
    21001 gtcaacggag gtaggtcacg ttcagtttgt ccagaaatct ctactcccat ttgactgagt
    21061 ggaatagtta ctcggtccat aggacgtttg gataaggaat catcaccaaa catagtcgcc
    21121 tcaaagtctt gacctgctaa aacgcctgaa atcaaacgaa tcgaggttcc agaatttccc
    21181 atatccaaat catttttggg ggcttgaaga ccatcaaagc caacaccgtg tacttcaaca
    21241 atatcaccat tgtcttcaat ttttacaccc aaatcacgaa agacctgcat ggttgacaag
    21301 acatcttccc cacgtaaaat attgtggaaa cgggtaacac cttttgcaag ggaaccaaaa
    21361 ataatagaac gatgactgat tgatttatca cctgggacac gaatcgttcc ctttagtttt
    21421 tctacgcttc ttcttaattt catatcctta cctcaaacta gtatcacttt cattttatca
    21481 tatttat
//
LOCUS       DBNFCK010000023        21032 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.023, whole genome shotgun sequence.
ACCESSION   DBNFCK010000023 DBNFCK010000000
VERSION     DBNFCK010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 21032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21032
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.023"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            43..648
                     /locus_tag="AC1PIW_000667"
     CDS             43..648
                     /locus_tag="AC1PIW_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HIP6605624.1"
                     /translation="MLGWLDRLFTLLFFLMKLSAIYLVLLLLGGVVLGISPANGTIVY
                     LYDNYHMDASKYNFREAFGYFKEHFIRLNLGLGLVFLLIGLLFSGIWLLIQLPQTWWM
                     PAVLITNAFGLFYIFALYALFLKLQVHFEFSLKTGLQLAAVSLFLDWKALVKFLLGSL
                     VCGVLLFKLPLILFFFLPVLWLLFLYDAFDPVYKKVDKDYL"
     gene            645..2294
                     /locus_tag="AC1PIW_000668"
     CDS             645..2294
                     /locus_tag="AC1PIW_000668"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIP6605625.1"
                     /translation="MKKVLIHTLLKSYSLLVIGIVIFFASILSYISWYQYDTNRIRIQ
                     QEVADGLKEELDYYQGRVGNYLADYITDRDRLDSLYHYFQLSPSQYQVWLLSHPHLMV
                     KRISLQDSVRTLYLYYPFVEGVDIAPMDANTVFVSTSDLKSGKKVTAENYKAPQHSIS
                     MTLFSPSTTEGIATIYVTIDADLLEQYIQRKTDLPMTVRVQDSLDREFYSLRPDVASP
                     PLSYRMSGDLQIAVGVDQQYAIEKVGSLVGIIFLGSSCLVALLLLVLRRVFKRYEVQV
                     LDIVDTMQQIRGEDRHLRLQTDNKEQEMYLISSEINQMLDAMDQSIQDIYRLQLAQKD
                     ANMRALQAQINPHFLYNTLEFFRMYAVSKQMDELGDMIYEFSSLLRSSVTQAKMTTVA
                     EELSFCEKHSYICQIRYPRSIAYAYQMDPGCERVEIPRFVIQPLVENYFVHGVDLKRK
                     DNALSVKALKHGQDMEILIRDNGKGMNAETLEAYKHLLASRELSQENRSQSIGLRNVH
                     ERLLLYFGDRYQIELDSKEGEGVTYSILFEGVFPRGDEHDA"
     gene            2284..3576
                     /locus_tag="AC1PIW_000669"
     CDS             2284..3576
                     /locus_tag="AC1PIW_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIP6605626.1"
                     /translation="MMHKMLVVDDEYMILEGMKHLLPYHEYNVEIVYTAENAEEALDY
                     FSQHTVDLVLTDVSMPDMTGLEMVRRMKEQSSDTHIIIMSGFQEFEYARKAISLGAMD
                     YLLKPINKQELAKLLQQLPTLKKETDREYTKGFLAGTLTASEFLSHSQKTWFAARETP
                     NPMGYCREKNILHQVYYFQLTESRPEGSLYTEEVTPTMTIRQLIDKVERRLFYKQLSE
                     DLTATTTELYKDMEPLLRAGRLAKIVDVLPHLADKLRETTPAVYLTKQFFNQMMSDVF
                     YYFNEEDHQRLEDFYVKVEASRDLHQLMEYFCHYLKKISESSTYSPHIQEVVTIVRER
                     FAEELSLKDMSAHLYLNTVYLGQLIKKETGHTFAELLNHQRIKVAQQLLLNSQLGIEE
                     ICFQVGYTNVGYFYKIFKRICGESPKNYRQKIGQLQTE"
     gene            3589..4449
                     /locus_tag="AC1PIW_000670"
     CDS             3589..4449
                     /locus_tag="AC1PIW_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIP6605627.1"
                     /translation="MSKREELQHLFPKGRLVFEKTENGASIQLPLANQTWFSVDRKDL
                     TDREIELIDLLMNDSSAEQGHPWGRYFQGGGPLPQTLEKLQVIHAHVGHVSDEDGKVA
                     WLDMMAKLLPNLQGYYTGNGQDYLFILHQAPLFDVKEILRDTLATMEFDFGLRITLFL
                     GQIWPAKLVHSWPQLIEAEQSLFHHWRSSHHQSRLLTFGQLYLWAGQLHPVLREGLAS
                     LIAHQQLEKEIVALWQEGAVITKAAQSLYIHRNTLQYRLDKWFDWTGLQLKELTDLAV
                     CYMVIMDLSI"
     gene            4685..5815
                     /locus_tag="AC1PIW_000671"
     CDS             4685..5815
                     /locus_tag="AC1PIW_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6605628.1"
                     /translation="MVQLNLKNIYKKYPNSEHYSVEDFNLDIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGEAYIDGVLMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYSKDEIKKRVEEAAQILGLTEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRTEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRIEQIGSPEELYNRPVNKFVASFIGSPAMNFFTVTLQDGVLVGDGFKVELPEGR
                     RKHLEEKGYNGKSFTLGIRPEDIKASQLELDTYPSSIVEAEVVVSELLGAETMLYSKV
                     GSTEFVSRVDARDYHNPGDKVRLTFNLNKAHFFDDETSKAIV"
     gene            6103..6321
                     /locus_tag="AC1PIW_000672"
     CDS             6103..6321
                     /locus_tag="AC1PIW_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605629.1"
                     /translation="MKTLLKKIRLAALSILLYNLILILSIWLGKVSSKEEFMIAVAGN
                     AVMMGLSFVHLHNQVSDEFHGKVEEPSA"
     gene            complement(6318..7286)
                     /locus_tag="AC1PIW_000673"
     CDS             complement(6318..7286)
                     /locus_tag="AC1PIW_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HIP6605630.1"
                     /translation="MSLSLRTIKLIFATVLAIYLATVLGLSYTTAAGIIAILSVLDTR
                     KSSFKMARNRLFSTLLALTIAVLTFALFGFGIWTLGIYLALYVPLAYRFNWEAGIAPS
                     TVLVTHLLLEQDISLIFLGNELTLFLIGAGLALLFNLYMPSQEKKIQAYHDQVEDLLK
                     QILLRFEAFLLNGDGRNEAELITQLDKTLDEALKVVYLDRHNQLFQQTNYQVHYFEMR
                     AAQNKILRTMAGNINKCLLEGRENVILSSLFERAAQQLSRDNSAKELLLDIELFHATF
                     RERPLPQTREEFETRATLFQLLHDMEAFIRLKVDFYEVYKDQEPSI"
     gene            complement(7361..7750)
                     /locus_tag="AC1PIW_000674"
     CDS             complement(7361..7750)
                     /locus_tag="AC1PIW_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HIP6605631.1"
                     /translation="MTLIFGIVAGLIAFAIGGLWYGLIFRDAWIKASGIDMAKVEADR
                     QAGKNGQKEMVISLVIEVLTAVVVIFFIKTLGVSPLHAAGGMGVIAVLASLKNYVFEQ
                     RPINLILINESYKLVCYLVVGILALFA"
     gene            7945..9162
                     /locus_tag="AC1PIW_000675"
     CDS             7945..9162
                     /locus_tag="AC1PIW_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HIP6605632.1"
                     /translation="MDQWMRDFEERQRHDELKQKMDAQTSAMNSQAIALQKQNNILQK
                     EHERQIEKEFKDRIFQLRLHLAQVTDESQRFVIQQMLSEAEEDYENYQREQAEKRLAD
                     AKRRKIWLIISSVVAVLGMAGLYFVYQDISQVTVPELYGIELSEAKDLLYEEGLKVGT
                     ITEVGGQSVQPGMVSVSSPSSGKKVKRGSAVNLIIAKNVDAKDTTVVEDSSTSEVATS
                     KTAGSSQASSQSQVGSAVQLFTVDIKKQGLVIRQEPSLSGEVVTNIEPGTYTIVETTQ
                     KEGHSWGRLKSGQGWISLTDLHNPVPQVDTKNLTTDQVKAWVMDDLFSRSVMIEKYRK
                     DQVVISVRMAEDGLVYADVTVPSDPNFEPRNWSYRINSRGELEASEYDTDPEQGGVNW
                     YVAQHYYLENSVN"
     gene            9218..9946
                     /gene="rlmB"
                     /locus_tag="AC1PIW_000676"
     CDS             9218..9946
                     /gene="rlmB"
                     /locus_tag="AC1PIW_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HIP6605633.1"
                     /translation="MEQNDIVYGVHAVVESLEANTGNKLYIQDDLRGKNVEKIKALAA
                     EKKVSISWTPKKTLSDMTEGAVHQGFVLRVSEFAYADLSVILEKAEQEDNPLILILDG
                     LTDPHNFGSILRTADATQVAGIIIPKHRAVGVTPVVAKTSTGAVAHVPIARVTNLSQT
                     LDKLKEAGFWVFGTDMNGTPSHKWNTAGKLALIIGNEGKGISSNIKKQVDEMITIPMK
                     GHVQSLNASVAAAVLMYEVFRKKI"
     gene            9947..10468
                     /locus_tag="AC1PIW_000677"
     CDS             9947..10468
                     /locus_tag="AC1PIW_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIP6605634.1"
                     /translation="MKEKVLIVDGYNMIAFWQATKQDFKKGDLDAARTTLLRTLSHYA
                     AFEQIEVICVFDAHHVPGLRQQYDEFKVSVIFTEEEETADSYIERLSAQLNSDRRKQV
                     SVATSDLNEQWVVFSQGALRVSARELEERTKVIKKDLDKFVDQVELYTPRLNPWSDGN
                     FQALKEMMEEMKE"
     gene            10465..11328
                     /locus_tag="AC1PIW_000678"
     CDS             10465..11328
                     /locus_tag="AC1PIW_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIP6605635.1"
                     /translation="MTFTIVTDSTSDLPISWVQENDVTVLGLTINLDGATYETVGENR
                     LTSADLLEKMASGSQPTTSQVNVGQFEEVFEAAAKEDHEVLYLAFSAALSGTYQSAVI
                     ARDMVMDQYPEAVITIVDTKAATIGEGYLVMKAVEARAAGKTLAETKAIVEDLVPRLR
                     TYLLVDDLNHLVRGGRLSKAAALIGGLVNIKPLLALNAEGKLEAIAKIRGRKKGIKEM
                     LNLTLDNLDHSTVMVAYTGDIEAAEGVKSSLLEDDRISDVLLTELGPVIATHTGTGVL
                     AILSIGTAMRA"
     gene            11793..12239
                     /gene="rplM"
                     /locus_tag="AC1PIW_000679"
     CDS             11793..12239
                     /gene="rplM"
                     /locus_tag="AC1PIW_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIP6605636.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKSISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            12259..12651
                     /gene="rpsI"
                     /locus_tag="AC1PIW_000680"
     CDS             12259..12651
                     /gene="rpsI"
                     /locus_tag="AC1PIW_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIP6605637.1"
                     /translation="MAQAQYTGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTQGSYDVFVNVNGGGYGGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            12964..13417
                     /locus_tag="AC1PIW_000681"
                     /pseudo
     CDS             12964..13417
                     /locus_tag="AC1PIW_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647366.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
     gene            13508..13849
                     /locus_tag="AC1PIW_000682"
     CDS             13508..13849
                     /locus_tag="AC1PIW_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HIP6605638.1"
                     /translation="MREDIQINERALILSDQLVEVLESIHDPEIELDIYNLGLVYEIH
                     LDETGFCKVVMTFTDAGCSCADTMPGELVAALKTIDGIEDAQVEIVWSPAWKMTRISR
                     LGRITLGISPK"
     gene            complement(13868..14263)
                     /locus_tag="AC1PIW_000683"
     CDS             complement(13868..14263)
                     /locus_tag="AC1PIW_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6176 family protein"
                     /protein_id="HIP6605639.1"
                     /translation="MKLNVELSRFRVKEGKTVQVDEWMAFLNEHMEDTLLTLEGEKMY
                     VETIFREVLDGREYLYWYSVQAEGGIEVEDSESYIDKKHLEYWEECIDPSYGMVDLDP
                     QVLMIPKPIYETMEELDRQYDDTFKKELQ"
     gene            complement(14314..16029)
                     /gene="ilvD"
                     /locus_tag="AC1PIW_000684"
     CDS             complement(14314..16029)
                     /gene="ilvD"
                     /locus_tag="AC1PIW_000684"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIP6605640.1"
                     /translation="MTDTNKLKDFRHRSSVYDSMVKSPNRAMLRATGMTDDSFEKPIV
                     GVISTWAENTPCNIHLHDFGKLAKEGVKEAGAWPVQYGTITVADGIAMGTPGMRFSLT
                     SRDIIADSIEAAMGGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAVFAYGGTIAPGNL
                     NGKDIDLVSVFEGIGKWNNGDLTAEEVRQIECNACPGPGGCGGMYTANTMATAIEVMG
                     MSIPGSSSHPAESPEKKADIEEAGRAVVRMLELGIKPSDIMTREAFEDAITVTMALGG
                     STNATLHLLAIAHAANVDLTLEDFNDFQERVPHLADLKPSGKYVFQDLYNVGGVPAVM
                     KYLLKNGFLHGDRITCTGKTVAENLKNFADLTPGQDVIMPLENPKRADGPLIILKGNL
                     APEGAVAKVSGVKVRNHTGPAKVFDSEEEAIEAVLTDEIVDGDVVVVRYVGPKGGPGM
                     PEMLSLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDLV
                     TVDQDTKEITMHVSDQEIEERKKTTVIPPLYSRGVLGKYAHTVSSASKGAVTDFWRPE
                     RTGKK"
     gene            complement(16190..16606)
                     /locus_tag="AC1PIW_000685"
     CDS             complement(16190..16606)
                     /locus_tag="AC1PIW_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605641.1"
                     /translation="MTLSTSVTNSTTRVSRTFISYLAITAFLFIFSRIYESLSYGEVS
                     VFMHYMFCVPLIGGAVLLGLLQLIPTLSRLSYNLWNSGVATITAGCLLRGIINLSGRS
                     TTLDQPYWYIGAAFLVLSLVGLFINPSANNHRKTTS"
     gene            complement(16581..17519)
                     /locus_tag="AC1PIW_000686"
     CDS             complement(16581..17519)
                     /locus_tag="AC1PIW_000686"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HIP6605642.1"
                     /translation="MKLLKKPFAYASIFGMLLTGGFTYSMLKTFVLSEAITTVAASNA
                     TSTSEQNTTTASSSTTATTATNVSTTDTSYTDDNIQINLETITTNNTTVYVADIQVSS
                     PEYLKTALAQNTYGTNVTAKTSETAAANNAILAVNGDYYGANSTGYVIKNGVLYRDTV
                     RDNAAYGDLAIYADGSFEVIYENEITAQELIDKGVVNLLAFDPSLVENGEIVVDTSTE
                     VGRAMSSNPRSAIGIIDENHYIIVVADGRTSESQGLSLYQLAEVMKQYGAQTAYNLDG
                     GGSSTLYFNGQVINNPTTNGNTISERAVSDIVYIGY"
     gene            complement(17506..18558)
                     /locus_tag="AC1PIW_000687"
     CDS             complement(17506..18558)
                     /locus_tag="AC1PIW_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HIP6605643.1"
                     /translation="MYYVVIPAYQPDEKLIQLLKIMKNRLNCQVIVVDDGSTGASKNI
                     LEIAKTYAIVLHHDVNKGKGQALRTAFSFIQDLRKPGVAVTADSDGQHAVNDIDRVAR
                     AAMNLPSRLILGVRQFTKDIPLRSRFGNKLTRVLFRLQTGVNVSDTQTGLRGFHTDLI
                     PFMLDIEGDHYEYKMNMLTQASQRYKITEVPIQTIYIDDNASSHFRPIKDGLLIYKNL
                     FKFALASFGGFLVDYGIYALALLLLTSLPTALRLLVANTIARICSATCNFALNKKLVF
                     NNEESIARTGVGYFTLALVLFLCDTALLYLFHQILGLNLYIVKLAVGIFLFLASWFIQ
                     KNIIFKERKSFSHEIA"
     gene            18783..20486
                     /locus_tag="AC1PIW_000688"
     CDS             18783..20486
                     /locus_tag="AC1PIW_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HIP6605644.1"
                     /translation="MQEIQLDQPRSGSYLILDTLHQLGVDLVFGYPGGAVLPLYDAIY
                     QYEGIQHILARHEQGAVHEAEGYAKSSGKVGVAIVTSGPGATNAITGIADAMGDSVPL
                     LVFTGQVATRGIGKDAFQEADVLGMTMPITKYNYQIRDTADIPRVITEALHIATTGRP
                     GPVVIDVPKDIQERVVDFYHDPTLHLPSYQPTIEPNGLQVKKILKQLSQAQKPVILAG
                     GGVNYADANKELVAFAERYRIPVVSTLLGLGAMPIEHELSLAMGGMHGSYAANMAMDQ
                     ADYIINIGARFDDRLTGNPTTYAPNATVAHIDIDPAEIGKVVKTAIPVVGDAKATLKA
                     LLALETVETGYADWTAQVLENKRRAPFWYDEDEKVIKPQAAIELIGQLTQGDAIVVTD
                     VGQHQMWAAQFYPYRHARQLVTSGGMGTMGFGIPAAIGAKLANPDKEVIIFVGDGGFQ
                     MTNQELAILNGYGVPIKVVLINNHSLGMVRQWQESFYEKRRSQSVFDDEPNFQLLAEA
                     YGISHYSFDNPATLSEDMKVILEDKPMLIEVHISNREHVQPMVPAGKSNAEMLGVKFN
                     A"
     gene            20479..20955
                     /gene="ilvN"
                     /locus_tag="AC1PIW_000689"
     CDS             20479..20955
                     /gene="ilvN"
                     /locus_tag="AC1PIW_000689"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HIP6605645.1"
                     /translation="MRRMLTAKLRNSSGVLNRFTGVLSRRQINIESISVGPTEVDGIS
                     RVTVIVDVVSHDEVEQIIKQLNRLIDVVRVRDLTDIPHLEREVILVKISAPPTKRAEI
                     LAIIQPFRANVVDVAPHSITIQMTGDGDKIDALLRVIQPYGIKNIARTGATGFSRD"
BASE COUNT         6104 a         4208 c         4857 g         5863 t
ORIGIN      
        1 accgattttt tgataaaata gataaaaaag gaggaatata caatgttagg gtggttagac
       61 aggcttttta ctcttctgtt cttcctcatg aaattgagtg ccatctattt ggttcttcta
      121 ttgctaggag gcgtagtgct gggcatttca ccagcaaatg gaacgatagt ttacctctac
      181 gataactatc atatggatgc atctaagtat aattttcgag aggcgtttgg ctattttaaa
      241 gagcatttta ttcggcttaa ccttggcctt ggtcttgtct tcttattgat tggtctttta
      301 tttagcggca tttggctctt gattcaattg ccccagacct ggtggatgcc agcagttctt
      361 attacaaatg cttttgggct tttctatatt tttgcactct atgctctctt tttaaaactt
      421 caagtacatt ttgaatttag tctaaaaaca ggcttacagt tggcggctgt ctcgcttttt
      481 cttgattgga aagccctggt caaatttttg ttaggcagtc tcgtctgtgg ggtcttgcta
      541 ttcaagcttc ccttgatttt attcttcttc ctaccggtcc tatggctctt gtttctctat
      601 gatgcctttg atccagttta taagaaagtt gacaaggatt acttatgaaa aaagttttaa
      661 ttcatactct tttgaaaagt tattctttat tagtgattgg gattgttatt ttctttgcca
      721 gtattctttc ctacatcagc tggtatcagt atgataccaa tcgcatccgc attcaacagg
      781 aagtagcaga tggtctaaaa gaggagttgg attattatca aggacgggtt gggaattatc
      841 tagcagacta cattacggac agggatcgtc tagacagcct ttatcactat tttcaattat
      901 ctccttctca gtaccaggtc tggttactga gtcacccaca cctgatggtc aaacggattt
      961 ccttgcagga ttctgttcgc actctctatc tctattatcc cttcgtagaa ggagtcgata
     1021 ttgctccgat ggatgcaaat actgtctttg tttctacctc tgacttaaag tctggtaaaa
     1081 aggtcacggc cgaaaattac aaggctccgc aacatagtat ttcgatgacc ttattttctc
     1141 catcgacaac agaaggtatt gcgaccatct atgtcaccat cgatgcggat ttgctcgaac
     1201 aatatattca acgtaagacg gatctcccca tgaccgttag agttcaagat tctctggatc
     1261 gggaatttta tagtctgcga ccagatgttg cctcacctcc gctctcctat cgaatgagtg
     1321 gggatttgca aattgcagtt ggagttgacc agcagtatgc tattgaaaaa gttggcagcc
     1381 tagtcggaat catttttcta ggaagtagtt gtctcgttgc tcttttacta ctggtcctga
     1441 gaagggtgtt taaacgatat gaagttcaag tactggacat tgttgatacc atgcaacaga
     1501 ttcgtggcga agacaggcat cttcgtctac agacggacaa taaagagcag gaaatgtatc
     1561 ttatttcatc agaaatcaat cagatgctgg atgctatgga ccagagtatt caagacatct
     1621 atcgcttgca gttagctcag aaagatgcca atatgcgagc cttacaagcc cagattaacc
     1681 cccattttct ctacaatacc ttggaatttt tccgtatgta tgcagtcagc aagcagatgg
     1741 atgagctagg ggatatgatt tatgaatttt caagcttgct tcgcagtagt gtgacacagg
     1801 caaaaatgac aacggtggca gaagaacttt cattttgcga aaaacatagc tacatttgtc
     1861 aaatccgcta tccccgttcc attgcctatg cctatcaaat ggatcctggg tgcgaaaggg
     1921 ttgaaattcc tcgttttgta atccagcctc tggtagaaaa ttattttgtc catggagtag
     1981 acctcaaacg aaaagacaat gcgcttagtg tcaaagctct caagcatgga caggatatgg
     2041 aaattctcat cagagataat ggtaagggaa tgaacgcaga aactctggaa gcctacaaac
     2101 acctcttggc aagtcgagaa ctgtcgcaag aaaatcggtc acaatcaatc ggattacgaa
     2161 acgttcacga gcggttactt ctctattttg gagaccgtta ccagatagaa ttagattcta
     2221 aagaagggga aggggtgaca tattcaatct tatttgaagg tgtcttcccc cgaggagatg
     2281 agcatgatgc ataagatgtt agtagttgat gacgaatata tgattttgga agggatgaag
     2341 catcttctcc cttatcatga atacaatgtc gagattgttt atacggcaga gaatgccgaa
     2401 gaagcgctgg attatttttc acagcatact gtggaccttg tcctaaccga tgtcagcatg
     2461 ccagatatga caggcctaga aatggttcga cgaatgaaag aacaatcgtc tgatacccat
     2521 attatcatca tgtctggttt tcaagaattc gaatatgccc gtaaagctat ctccctaggg
     2581 gcaatggatt atctccttaa gcccatcaat aaacaagaac tggctaagct gctccaacag
     2641 ctaccaacct tgaagaaaga gacagatagg gaatacacca agggcttctt ggcaggaacc
     2701 ctgacagcga gtgagttctt aagtcactcc cagaaaacat ggtttgccgc tagagagact
     2761 ccaaacccaa tgggctactg tcgtgaaaaa aatattttac atcaagtcta ctattttcag
     2821 ttgacagaaa gtcgaccaga gggtagtcta tatacagaag aagttacccc gacgatgacc
     2881 atacggcagt tgattgacaa ggtagagagg cgcttatttt ataagcaact atcagaagat
     2941 ctaacggcta ctaccactga actctataaa gatatggaac cgcttctacg tgcaggaaga
     3001 ttggcaaaaa tcgttgatgt tttaccacat ttggctgata aattacggga aaccactccg
     3061 gcagtctacc taacaaaaca attttttaat cagatgatgt cggatgtgtt ttactatttt
     3121 aatgaagaag accatcagcg cttggaagat ttctatgtaa aggtagaagc gagtcgagat
     3181 ttacatcaac taatggagta tttttgtcat tatctgaaaa aaatttccga gagttccact
     3241 tacagtccac atatacagga agtcgttacg attgttcgtg aacgctttgc agaggaactg
     3301 tctttgaaag atatgagtgc tcatctctac ctaaatacgg tttacctagg tcaacttatc
     3361 aaaaaagaga ccgggcatac ttttgcagaa ttgctcaatc atcagcgaat caaggtagcg
     3421 caacagcttc tcttgaacag tcagttggga atcgaagaaa tttgttttca agttggatat
     3481 acaaatgtcg gttacttcta taagattttt aaacgcattt gtggggaatc tcccaaaaat
     3541 taccgacaga aaatcggtca attacagaca gaatgagatg gaggcgagat gagcaaaagg
     3601 gaagaattgc agcacctatt tccaaaagga aggcttgtat ttgaaaagac agaaaacgga
     3661 gcaagtatcc agttgcctct ggctaatcaa acctggtttt ccgtcgatcg caaggatttg
     3721 acagatcgag aaatcgaact gattgacttg ctgatgaatg actcgagcgc agaacaaggt
     3781 catccttggg gacgctattt ccaaggggga ggtcctcttc cacaaacctt ggaaaaacta
     3841 caggttattc atgctcatgt aggccacgta tccgatgaag atggaaaggt agcctggctt
     3901 gatatgatgg ccaaactcct ccccaatctc caaggctact atacaggcaa tggacaagat
     3961 tacctgttca ttctacatca ggcacctctt tttgacgtca aagaaatact gcgtgacacc
     4021 cttgctacca tggagttcga ttttggtctg agaattacat tgtttttagg gcaaatctgg
     4081 ccagccaaat tggtacactc ttggccgcaa ttaatcgaag ctgaacaatc cctttttcac
     4141 cattggcgaa gcagccacca tcaatcacgg ttgcttacct tcggtcagct ctatctttgg
     4201 gcaggtcaac tccaccccgt tttgagagaa ggacttgcta gtttgattgc ccaccagcag
     4261 ctagaaaagg aaattgtagc tctttggcaa gaaggtgcgg tcataaccaa ggcagcccag
     4321 tctctctata tccatcgaaa tacgctacag tatcgcttgg acaaatggtt tgattggacg
     4381 ggattacagc tcaaggaatt gacggatttg gcagtttgct acatggttat catggattta
     4441 agtatctaat aggagctaat gatgagaaaa gcgttgtagc ctttgagaat gccacccatc
     4501 gcttcgtccc ttttctactg aaaatcccaa agggctcatt gaatttttgg aaagtaaact
     4561 atttcagtaa cacagtcttt tgtgcaactt gtacaaaaga ttgtgttctt tttgttcacc
     4621 ttgacctaga aaaataaagc gttttcattt acaataaaac tatcaaagaa agcgaggatt
     4681 gaacatggtt caattgaatt taaaaaatat ctataaaaaa tatccaaaca gcgagcacta
     4741 ctctgttgaa gactttaact tggacatcaa ggacaaggag tttatcgtat ttgtaggtcc
     4801 ttcaggatgt ggtaaatcaa caactcttcg tatgattgct ggtttggaag atatcacaga
     4861 aggggaagca tacatcgacg gcgttctcat gaatgatgtg gctccaaaag accgcgacat
     4921 tgccatggta ttccagaact atgccctcta cccacacatg actgtatttg acaacatggc
     4981 ctttggtttg aaattgcgta aatacagcaa ggatgaaatc aaaaaacgtg tagaagaagc
     5041 tgctcaaatt cttggtttga cagaattctt agaacgtaaa ccagctgacc tttcaggtgg
     5101 acaacgccaa cgtgttgcca tgggtcgtgc catcgttcgt gatgcaaaag tattcttgat
     5161 ggatgaacct ttgtcaaact tggatgcaaa actgcgtgta tccatgcgta cagagattgc
     5221 taaaatccac cgccgtatcg gtgcgacaac catctatgta acccacgacc aaacagaagc
     5281 tatgaccttg gcagaccgta tcgttatcat gtctgcaact aaaaacccag caggcacagg
     5341 tacaatcggt cgtatcgagc aaatcggtag cccagaagag ctctacaacc gccctgttaa
     5401 caagtttgtt gctagcttca tcggtagccc agctatgaac ttcttcacag ttacgcttca
     5461 agatggcgtt cttgttggag atggtttcaa ggtggagctt ccagaaggac gtcgcaaaca
     5521 cttggaagaa aaaggctata atggcaaatc atttaccctc ggtatccgtc cagaagacat
     5581 caaggcttct cagttggaat tggacactta tccatcttcc atcgtagaag cagaagtggt
     5641 tgtatcggag cttctcggtg cagaaaccat gctctactct aaagtaggta gcacagagtt
     5701 tgtatctcgc gttgatgcac gcgactacca caacccagga gacaaagttc gcttgacctt
     5761 caacctcaac aaagctcatt tctttgatga tgaaacaagc aaggcgattg tctaaccttt
     5821 ctaaaatcgt tcataaaaca cctgaccgct agatgaaaat ctggcggttt tttggggtta
     5881 agaacgcata aatacttgtt atttttatta aagaggctgg gcaaaaagcc cagttccact
     5941 tctcagagtt cgtgtcaaca tctcagcgca gtggttgatt ggcagatttg ttcgtgtttt
     6001 acactccaaa tctgacctaa tcaactgtgc gggggtggga aaacgaactc ttctatggat
     6061 ggtcgagttc tgtcccactc ccaacactta caggaggttc ctatgaagac actactgaaa
     6121 aaaattcgtt tggcagctct ttctatcctt ctttacaatc tcattttaat cctctccatt
     6181 tggctaggaa aggtatcttc caaggaagaa tttatgattg cagttgcagg gaatgcagtc
     6241 atgatgggcc tatctttcgt gcatttgcac aaccaagtat cagatgaatt tcatggaaaa
     6301 gtagaagaac cgtcggctta aattgacggt tcttggtctt tatagacttc ataaaaatca
     6361 accttgaggc gaataaaagc ttccatatca tgcaggagtt gaaagagggt agcacgggtt
     6421 tcaaattctt cccttgtttg tggcagaggt cgctcgcgga aggtggcatg gaatagttcg
     6481 atgtctagga gcagttcctt ggcggagttg tctcggctca gttgctgggc tgctcgctcg
     6541 aagaggctgg acaggatgac attttctctg ccttccagta aacacttgtt gatatttcct
     6601 gccatggttc gcaagatttt gttttgtgct gcccgcattt caaagtaatg gacctggtag
     6661 ttggtctgct ggaagagctg gttgtggcgg tctaggtaga cgaccttcag ggcctcatcc
     6721 aaggtcttat ccagctgcgt aatcaattct gcctcgtttc gtccgtcgcc gttgagcaga
     6781 aaggcttcaa agcggaggag aatctgcttg agtaggtctt cgacttggtc atggtaggcc
     6841 tggattttct tttcttgcga gggcatatag aggttgaaca agagtgccag tcctgcccca
     6901 atcagaaaaa gcgtcagctc atttcccaaa aaaatcagcg aaatatcttg ctctagcaag
     6961 agatgggtga cgaggacggt cgatggggcg atgccagcct cccagttgaa acggtaggcc
     7021 agagggacgt agagtgctag gtagatgccc agggtccaga tgccaaagcc aaagagggcg
     7081 aaggtcagga ctgctatggt cagggccagg agggtggaaa agaggcggtt gcgggccatt
     7141 ttgaagctgg acttgcgggt gtcgaggacg ctgagaatgg cgatgatgcc tgccgctgtc
     7201 gtataagaca ggcccagtac ggtcgctagg taaatagcca gaacggtggc aaatatcagt
     7261 ttgatggtgc ggagggagag ggacatgagg gctcctttgg ttttagtcta tttctattgt
     7321 atcataaaat aaaaacctcc taaggatagg agggaattac ttatgcaaat agagctagaa
     7381 taccgactac taggtagcag actagtttgt agctttcgtt gatgaggatg aggttgatag
     7441 gacgttgctc aaagacgtag tttttcaatg aagcaaggac cgcaattacg cccatgccac
     7501 cagctgcatg aagtggtgaa acaccgaggg tcttgatgaa gaaaataacc acaacagcag
     7561 tcagtacttc aatgacaagg gaaatcacca tttccttctg tccatttttg ccagcctgac
     7621 gatctgcttc taccttagcc atgtcaatgc ctgaagcctt gatccaagca tcgcggaaaa
     7681 tcaaaccata ccacaaacca ccaatagcaa aggcaatcag ccccgccaca attccaaaaa
     7741 taagtgtcat aaaatctcct ttttcgattc cgtttgtacg gacaaatagt agatgttaga
     7801 atatcatagg ctttactatt tgtcaaaagt tagattgaaa cggggtgaaa atgaggattg
     7861 acaaattaag gtgttatgct atactaaaat aaaatagatt ttatttgtag ataataacca
     7921 atattttatg aggagatgat tattatggat caatggatgc gagattttga agagcggcag
     7981 agacatgatg aattgaagca aaaaatggat gctcaaacct ctgctatgaa ctctcaagca
     8041 atagcacttc aaaaacaaaa taacattttg caaaaagagc atgagcgtca aattgaaaaa
     8101 gagtttaagg atagaatttt tcaattacga ttgcacttag cacaggttac ggatgagagt
     8161 caacgatttg tcatccagca aatgcttagc gaagctgagg aagattatga gaattatcaa
     8221 agagagcaag ctgagaaacg tttggcagac gcaaaaagac gaaagatatg gctaatcatt
     8281 tcaagtgttg tggctgtgtt aggaatggct ggcttatatt ttgtatacca agatatttct
     8341 caagttacag ttcctgaatt atatggtatt gagctatctg aagctaagga tttactgtat
     8401 gaagaggggt taaaagttgg tacaataaca gaagttggtg gacagtcagt tcagcctggg
     8461 atggttagtg tatcttctcc aagtagtggg aagaaggtaa agcggggaag tgcggttaac
     8521 ttaattattg ctaaaaatgt agatgcaaag gatactaccg tagttgagga tagttcaact
     8581 agtgaggtag ccactagtaa gactgctgga agttcgcaag cttctagcca gtctcaggta
     8641 ggtagcgccg ttcaattgtt tactgtggat attaaaaaac aaggactggt tattcgtcag
     8701 gaacctagtt tatctggaga agttgtgacc aatatagaac ctggaacgta cacgatagtt
     8761 gagacgactc aaaaggaagg acatagttgg ggcagattaa agtcaggaca gggttggatt
     8821 tcgttgacag atttgcataa tcctgtaccg caggttgata ccaaaaactt aacgacagat
     8881 caggtaaaag cttgggttat ggatgattta tttagccgta gcgtcatgat tgaaaagtat
     8941 cgaaaagacc aggttgtcat aagtgttcgt atggctgagg atggtttagt ttatgcagat
     9001 gtaactgtac catcagaccc taattttgag ccacgaaatt ggagttatcg gatcaatagt
     9061 cgtggggagt tggaagctag tgagtatgat acagatccgg aacaaggggg tgtcaactgg
     9121 tatgttgccc aacattatta tctggaaaac agtgtgaact aagaaagctg agaaattctc
     9181 ggctttttcc ttttcaaaaa tatgctataa taaaaccatg gaacagaatg atatcgtata
     9241 tggcgtacat gctgtggtgg agagtttgga ggctaacacc ggcaacaagc tctacatcca
     9301 ggatgatttg cgcggaaaaa atgttgaaaa aatcaaggcc ctggcggcag agaagaaggt
     9361 gtcgatttct tggacgccca agaagaccct gtctgacatg acagaaggtg ccgttcacca
     9421 aggctttgtc ctgcgggtct ctgagttcgc ctatgcggac ctgtcggtga ttctggaaaa
     9481 agcagagcag gaagacaatc ccctcattct catcttggac ggtctgacag acccccacaa
     9541 cttcggctcg attttgcgga ccgcagatgc cactcaggta gctggcatca tcattcccaa
     9601 gcaccgagca gttggcgtga cgcccgttgt ggcaaagacc tcaacaggtg cggtggccca
     9661 tgtcccaatc gcccgagtga ccaatcttag ccaaactctc gataagctca aggaggctgg
     9721 tttctgggta tttggcacgg atatgaacgg aactccaagc cacaagtgga atacagctgg
     9781 caaactagct cttattattg gcaacgaagg caagggcatc tccagcaata tcaaaaagca
     9841 ggtggatgag atgattacca ttcctatgaa gggccatgtc caatctctca acgccagcgt
     9901 agcagcagct gtgctgatgt acgaagtatt tcggaagaaa atctaaatga aagaaaaagt
     9961 tctcattgta gatggctaca acatgattgc cttttggcag gccaccaagc aagattttaa
    10021 aaagggagac ttggatgcgg ccagaacgac cttgcttcgc accttgtctc actacgcagc
    10081 ctttgaacag attgaggtga tttgtgtctt tgatgcccac catgtgccag gtcttcgcca
    10141 gcagtatgac gaattcaagg tatcggtcat ttttacagag gaggaagaga cggcagatag
    10201 ttatatcgaa cgactcagtg cccagctcaa tagtgaccgt cgcaagcagg tttccgtcgc
    10261 aaccagcgac ctcaacgaac aatgggtggt cttttcacaa ggggccttac gggtttcggc
    10321 gcgtgagtta gaggagagaa ccaaggtcat caaaaaggac ttggacaagt ttgtggacca
    10381 ggtagaactc tacaccccac ggctcaatcc ctggtcagat gggaattttc aagccttaaa
    10441 agaaatgatg gaggaaatga aagagtgaca tttacaatcg taaccgattc gacatcggat
    10501 ttgccaatca gctgggtcca agaaaacgat gtgaccgtgc ttggtttgac cattaaccta
    10561 gacggcgcaa cctatgaaac agtcggtgaa aaccgcttga cctctgcaga tttactggag
    10621 aaaatggctt ctggcagcca acccacgacc agtcaggtca atgttggtca gtttgaagaa
    10681 gtttttgaag cggcagccaa ggaagatcat gaggtgcttt atctcgcttt ctcagcagct
    10741 ctttcaggga cctaccagag tgcagttatt gcacgagata tggttatgga ccaatatcca
    10801 gaagcagtca tcaccatcgt tgatacaaag gcagcgacca ttggagaagg ctacttagtc
    10861 atgaaagccg ttgaggcacg tgctgcaggt aagactctgg cagaaaccaa ggcaatcgta
    10921 gaagatttgg tgccgcgttt acgaacctac ctcttggtcg atgaccttaa tcatttggtg
    10981 cgaggtggac gcttgtctaa agcggcagcc ctcattggtg gattggtcaa tatcaaacct
    11041 ctccttgcac tcaatgcgga agggaagtta gaagccattg ccaaaatccg tggtcgtaaa
    11101 aaaggaatta aggaaatgct gaacttgacc ttggacaact tggatcattc tacggtcatg
    11161 gtggcctata caggtgatat tgaggcggca gagggcgtca aatctagttt attggaagac
    11221 gaccgtattt ctgatgttct attgacagaa ttagggcctg tgattgccac tcatacaggg
    11281 acaggagtct tagctatttt atcaattggt acagcaatga gagcctagat tctaagggat
    11341 atttcttgta aagatgtgtc cctttttgtg tgatgacgac ttatgtagaa tagggtaatc
    11401 aaaaggaggt acgataatgc tcattgtcaa gttgttccaa ccagaagcct tggatggctg
    11461 aaactggtgt tttacaggtc ctctggtcta tacggtggat gaattggtct tggaagaatt
    11521 tgataaaacc tttccttata aggagaagct ggtcttgcct ggacaactag ggcagtaaga
    11581 aatttaaaat ggataaggct ttcattctca gcagctctgc tatctttcaa aaaataaaaa
    11641 aagtaaagta aaatagcttg acggacacta gtagatttga taaaatggta gatggtattg
    11701 tttaccccat ttgaaaggcc ccggaacctt ccaaataccc gcggaccgga acatccgccc
    11761 agtaaacaaa aacgattcgt ataggagaaa tcatgaacaa aacaacattt atggctaaac
    11821 caggccaagt tgaacgtaaa tggtatgtag tagacgctac tgatgtacct cttggacgcc
    11881 tttcagcagt agttgctagc gttcttcgtg gtaaaaacaa accaactttc acacctcaca
    11941 ctgatactgg tgacttcgtt atcgtaatca acgctgagaa agtgaaattg actggtaaaa
    12001 aagcaactga taagatctac tacactcact caaaccaccc aggtggcttg aaatcaatct
    12061 cagctggtga attgcgttct aaaaacgctg ttcgtttgat tgagaaatca gttaaaggta
    12121 tgcttccaca caacacactt ggtcgtgcac aaggcatgaa gttgaaagtg tttgtaggtg
    12181 ctgagcacac tcacgctgca caacaaccag aagtacttga tatttcaggt cttatctaag
    12241 ggaaaggacg taatctaaat ggcacaagca caatacacag gtactggtcg tcgtaaaaac
    12301 gcggttgcac gcgtacgttt ggtcccaggt actggtaaaa tcacagtaaa caaaaaagat
    12361 gtagaagagt acatcccaca tgctgacctt cgtttggtta tcaaccaacc attcgcagta
    12421 acttcaacac aaggttcata cgacgttttc gttaacgtga acggtggtgg ttacggtggt
    12481 caatcaggtg cgatccgtca cggtatcgcg cgtgcattgc ttcaagtaga cccagacttc
    12541 cgcgattcat tgaaacgcgc aggtcttctt acacgtgacg ctcgtatggt tgaacgtaag
    12601 aaaccaggtc ttaagaaagc acgtaaagca agccagttta gtaaacgtta atataccgca
    12661 atatatcaac gtttcaaagc actcaagagt cgctttttta taattaggta gtttttatag
    12721 ttacaacgga ttttgattga tgtcataatc ggttcagagt caaatccata ccaattggaa
    12781 atcaaaaggg ttcaggaacc tttgaaggtt gaaaatgaag caaacatagc taccttctta
    12841 aatggttccg tcagtaggga gttgttcatg aaacactttc actatttcta tcaatattcc
    12901 acttaacatt ttcgcttgtt tttgatatac tttagaaaat aggtgttttc aaggggtgat
    12961 accatggaac taaaagatat tatgcatatt ttggaagata tgaaggttgg ggtttttgct
    13021 actttggatg agtatggaaa tccacatgct cgtcatgctc atatcactgc agcaaacgaa
    13081 gaagggattt tctttatgac aagcccagaa acgcattttt atgatcaatt gatgggggat
    13141 caaagagttg cgatgacggc tatttctgag gaagggtacc ttatccaggt agtgcgtgtg
    13201 tgtagaaggg gctgctagac ctgtggagaa cgactatcta aagacagttt ttgcggataa
    13261 tccttattat caacatatct ataaagatga gtctagtgat acaatgcaag tatttcagat
    13321 ttatgctggt catggcttct atcatagctt gactcaaggt cataagtata tcttttctat
    13381 tggtcaaggt gagaattcag aggttcgtgc actttgaaaa ctagttgaaa acatttggta
    13441 agacaagtat atttcaaata attatgatac aatacttttt ataaatgttt taggagtgtt
    13501 tggataaatg agagaagata ttcagatcaa tgagcgagcc cttattttgt cagatcaatt
    13561 ggtggaggta ttggaatcca ttcacgaccc cgagattgag ttggatatat acaatttagg
    13621 acttgtctat gaaattcatc tagatgaaac cgggttttgt aaagtagtta tgacctttac
    13681 tgatgcaggt tgctcatgtg cagatacgat gcctggagag ttagtagcag ctttgaagac
    13741 tattgacgga atagaagatg ctcaggtcga gattgtttgg tctcctgctt ggaaaatgac
    13801 aagaattagt cgcttaggtc gaattactct ggggataagc cctaaataaa agagttgcag
    13861 ttttatttta ctgcagctct tttttaaacg tgtcatcgta ttgtctgtct aattcttcca
    13921 tcgtttcata aatgggtttg ggaatcataa ggacttgagg atccagatcg accataccat
    13981 agcttggatc aatgcattct tcccagtatt ctaaatgttt tttgtcgatg taggattctg
    14041 aatcttcgac ttcaatcccc ccttcggctt ggacagagta ccaatagagg tattcgcgtc
    14101 cgtccaatac ctcacggaag atggtttcga catacatttt ctctccttca agagtgagaa
    14161 gggtgtcttc catgtgctcg ttgagaaagg ccatccattc atctacttga acggttttgc
    14221 cttctttaac acgaaagcgg gagagttcga cgtttagttt cattttttct ccttttggaa
    14281 aaccctaccg cgaggacagg gtcgttgagt tccttatttt ttccccgtac gttctggtct
    14341 ccagaagtcg gtaacggctc ctttagaggc agaggataca gtgtgggcgt attttccgag
    14401 aacgccacga gagtagagtg gtggaataac agttgttttc ttgcgttctt cgatttcttg
    14461 gtcagaaacg tgcatggtga tttctttggt atcttgatca accgtcacca aatcaccagt
    14521 acggaggtag gcaattggcc caccatcctg agcttcaggt gcgatgtgtc ccacaaccag
    14581 tccataggta ccaccagaga aacgaccgtc tgttagaagg gctaccttgt caccttggcc
    14641 ttttccgaca atcatagaag aaagggacag catttcaggc ataccaggac cgccttttgg
    14701 tccaacatag cggacaacga ctacatcgcc atctacgatt tcgtcagtca agactgcttc
    14761 gatagcttct tcttcagaat caaagacctt ggctggacct gtatggttgc ggactttcac
    14821 accagaaacc ttggcaacgg caccttcagg agcaaggtta cctttcaaga taatcagagg
    14881 tccatccgct cgttttggat tttcaagtgg catgatgaca tcttgacctg gagtcaagtc
    14941 tgcaaaattc tttaggtttt cagcaacggt tttacctgta catgtgatgc ggtcgccatg
    15001 aaggaagccg tttttgagca agtatttcat aaccgcaggg acaccgccga cattatagag
    15061 gtcttggaaa acatatttac cagatggttt taagtcggcc aaatgaggca cacgctcttg
    15121 gaaatcgttg aaatcttcca aggtcaaatc aacgttggca gcatgggcaa tggccagcaa
    15181 gtgcagagta gcgttggttg agccgcccag agccattgtg actgtaatgg catcttcaaa
    15241 ggcttcacgc gtcataatgt cagatggttt gatgccaagt tctagcatgc gtacaacagc
    15301 acgaccagct tcttcaatat cagccttctt ctcaggagat tcagcagggt gagaagaaga
    15361 gccaggaata gacatgccca ttacctcaat ggcagtagcc atggtattgg cagtgtacat
    15421 accaccgcaa ccaccaggtc cagggcaagc attacactcg atttgacgaa cctcttcagc
    15481 agtcaagtca ccgttgttcc attttccgat tccttcgaag acagaaacca agtcaatatc
    15541 tttgccattg aggtttcctg gtgcgatggt tccaccgtag gcaaatacgg caggaatgtc
    15601 catattggcg atggcaatca tggaaccagg catgttttta tcacagccac cgattgcgac
    15661 aaaggcatcc acgttgtgcc ctcccatggc tgcctcaata gaatctgcaa tgatatcacg
    15721 tgaagtcaag gagaaacgca tgccaggagt tcccatagcg attccgtctg caacggtgat
    15781 ggtaccgtat tggacaggcc aggcacctgc ttccttgaca ccttctttgg ctaattttcc
    15841 aaagtcatga aggtggatgt tacagggtgt gttttcagcc catgtcgaaa taaccccaac
    15901 aataggcttt tcaaagctat catcggtcat acctgtggca cgcaacatag cacggttggg
    15961 ggatttgacc atcgaatcgt aaacagagct acgatgtcga aagtctttaa gtttgtttgt
    16021 atcggtcata attttcttct ccctatctta ttttacccat tatatcatag atatgaaaga
    16081 aacgataaaa gatttgataa acaattggaa atttccgaat ttttcaacaa tttattctac
    16141 agtaaaaggc acctaagaat gttaagtgcc cttataattt atttgtagat taagaggtcg
    16201 tttttcgatg attatttgcg gatggattga tgaaaagacc gactaacgat agaactagga
    16261 aggctgcgcc gatgtaccaa taaggttgat ctaaggtagt tgaacgtcca gataggttga
    16321 taatgcctcg gagaagacaa ccagctgtaa tagttgcaac gccagaattc cacaaattat
    16381 aagagagacg tgaaagagta ggtataagtt gcagcaaacc gagtagcaca gctccgccta
    16441 ttaatggtac acagaacata taatgcataa agacagagac ttcgccatag ctgagacttt
    16501 catagattcg actgaaaata aacaggaaag ctgttattgc taagtaggag ataaacgttc
    16561 ttgaaactct tgtagttgaa ttagtaaccg atgtagacaa tgtcactcac cgccctttct
    16621 gagatagtat ttccatttgt tgttgggttg ttaataactt gaccgttaaa ataaagagtg
    16681 gatgaaccac cgccatcaag gttgtaggca gtttgagcac catattgttt cataacttcg
    16741 gccaattgat atagagagag tccttggctt tcagacgtac gtccgtctgc tacgacgatg
    16801 atataatgat tttcatcgat aatcccgata gcagaacgag ggttggatga cattgcacgt
    16861 ccgacttctg ttgatgtatc aacgacaatt tcgccgtttt cgaccaatga tggatcaaat
    16921 gctaagaggt tgacaacgcc cttgtcaatc aattcttggg cggtaatctc attttcataa
    16981 atgacctcaa atgagccgtc tgcgtagatg gccaagtcgc cataagctgc attgtcccga
    17041 acggtgtctc gatacagaac cccattctta ataacataac cagttgaatt ggctccatag
    17101 tagtcaccat taaccgctag aatggcattg tttgctgccg cagtttcaga agttttagct
    17161 gttacgttcg tgccataggt attttgggct agagctgtct ttaaatactc tggagagctg
    17221 acttgaatat ctgcgacgta aacagttgtg ttgttggtag tgatggtctc taagttgatt
    17281 tggatattgt catcagtgta actagtatcc gttgtagaga cattcgttgc tgttgtggca
    17341 gtagtggaag acgaagctgt agttgtattc tgctcgctcg tagaagtagc attacttgca
    17401 gcaaccgttg tgatagcctc ggaaagcacg aaggttttta acatggagta agtaaaacct
    17461 cctgtcaaaa gcattccgaa gatggatgcg taggcaaagg gttttttaag caatttcatg
    17521 ggaaaatgat ttcctttctt taaagatgat atttttctga atgaaccaag aggctaggaa
    17581 aaggaagata ccaactgcaa gtttgacaat gtagaggttg agtcctagaa tttggtggaa
    17641 gagatatagg agggctgtat cgcaaaggaa gaggacaagg gctagtgtaa agtagcctac
    17701 gcctgttcgt gcgatgctct cttcattgtt aaagactaat ttcttattaa gtgcaaaatt
    17761 acaggtagcg ctacaaatgc gagcaatcgt gtttgcgacc aataagcgta gagcagttgg
    17821 taagctagtg agtagcaata aggcaagtgc atatattccg tagtcaacta gaaatccacc
    17881 aaaagatgcc agggcaaatt tgaagagatt cttgtaaatg agcagtccat cttttatggg
    17941 acgaaaatgc gaactggcat tatcatcgat ataaatagtt tgaattggaa cttccgtaat
    18001 tttatagcgt tgacttgcct gagttaacat gttcatcttg tactcgtagt ggtcgccttc
    18061 aatatctaac atgaatggaa ttaagtcagt atggaatcca cgcaagcctg tttgggtatc
    18121 ggacacattg acaccagttt gcaatctaaa caatactctg gttagtttgt taccaaatcg
    18181 gctacggagc gggatgtctt tagtaaattg acgaacacca aggatgaggc ggcttggtag
    18241 attcatggct gcgcgtgcga cacggtctat atcattcaca gcgtgttgac catctgaatc
    18301 agcggtaaca gcgacaccag gctttctcaa atcttggatg aaactaaagg ctgttcgtaa
    18361 ggcttggcct tttcctttat tgacatcatg gtggagaaca atggcatagg ttttggcgat
    18421 ttcaagaata tttttggatg cgccagtact accatcatcc acaacgatga cctgacaatt
    18481 tagcctgttt ttcattattt ttaatagttg gattaatttt tcatctggct ggtaagccgg
    18541 tatcactaca taatacatat cctaaacctc atttaatttt tttagtggaa ctactatact
    18601 gatgttttct gaaagcaagc tgaaaccaag ccagtcaagg gatttgctta agttttgctt
    18661 aagaaatgtt aggaaatagt taagaaaacg aacaaatgta ggaattttca gaaaatttct
    18721 tgacattcta cttccttgtg atattatagt agataacaaa tttgcagaga ggaggaatat
    18781 tcgtgcaaga aattcaacta gaccaaccac gttcggggtc ctatctcatt ttggacactc
    18841 tgcatcagct aggagtagac cttgtttttg gttatcctgg tggggcagtt ttgcctctat
    18901 atgatgctat ctatcaatat gagggaatcc agcatatttt ggctcgtcat gaacaaggag
    18961 cggtccacga ggccgaagga tacgctaaat catcaggaaa ggtgggcgta gccattgtaa
    19021 cctctggacc aggtgctacc aatgccatta ctgggattgc ggacgcaatg ggtgatagtg
    19081 ttcctctttt agtttttact ggtcaagtcg caacacgtgg tatcggtaaa gatgcttttc
    19141 aggaagcaga tgtcttgggg atgacaatgc cgattaccaa gtacaattac caaattcggg
    19201 atacggcaga tattcctaga gtcatcacag aagcactcca tattgcgaca acagggagac
    19261 caggtcccgt tgttattgat gttccaaaag atatccaaga aagagttgtt gatttttatc
    19321 atgacccaac tctacaccta ccaagttatc agccaacgat tgaaccaaat ggtttgcagg
    19381 ttaagaaaat tttgaaacag cttagccaag ctcaaaaacc agttatattg gctggtggtg
    19441 gtgtcaatta tgcagatgcc aataaagaat tagttgcctt tgcagagcgc taccgtattc
    19501 cagtagtttc aaccctcctt ggattaggtg caatgcccat tgagcatgaa ttatccttgg
    19561 ctatgggtgg tatgcacggt tcatacgctg ccaatatggc tatggatcag gctgattaca
    19621 ttatcaatat tggagcgcga ttcgatgacc gcctaacagg aaatccaaca acctatgcgc
    19681 caaatgcgac ggtagcacat attgatattg atcccgcaga aattggtaaa gttgtcaaaa
    19741 cggctattcc ggtagtgggc gacgctaagg caaccttgaa agctctgctt gctttagaaa
    19801 cagtcgaaac aggctatgct gattggacgg cacaagtatt ggaaaacaaa cgtcgggcac
    19861 cattttggta tgatgaagat gaaaaggtta ttaagccaca ggcggcaatt gaattaattg
    19921 gtcagttgac gcaaggggat gccattgtcg taacagacgt tggtcagcat caaatgtggg
    19981 ccgctcaatt ctacccctac agacatgcac gccaacttgt aacatctggt ggcatgggaa
    20041 ccatgggctt tggtattcct gcagctatcg gcgccaaatt agccaatcca gataaagaag
    20101 taatcatctt tgttggtgat ggcggcttcc aaatgaccaa ccaagaattg gctattttga
    20161 atggctatgg tgtaccgatt aaggttgtgt tgatcaataa ccactctctt ggaatggttc
    20221 gtcaatggca ggaatccttc tatgaaaagc gtcgtagcca atctgtcttt gacgatgaac
    20281 cgaatttcca attgttggca gaggcctacg gaattagtca ttatagtttt gacaatccag
    20341 cgactctaag tgaagatatg aaagttatct tagaagataa accaatgctc atagaggttc
    20401 atatttctaa tcgtgaacat gttcaaccga tggtgccagc aggtaagagc aatgcagaaa
    20461 tgttgggggt gaagttcaat gcgtagaatg ttaacagcta aattgcgaaa ttcatctggt
    20521 gttctgaatc gttttacagg tgtcctatct cgtcgccaaa tcaatattga gtccatctcc
    20581 gtcggcccaa cagaagtaga tggcatctca cgtgtgacgg tgattgtaga cgtggtcagt
    20641 cacgatgagg tcgaacaaat catcaaacag ctaaaccgct tgattgatgt ggtccgtgtc
    20701 cgtgatttga cggacattcc ccacttagag cgtgaggtga ttcttgttaa aatctcagct
    20761 ccgccaacaa aacgagcaga aattttagcg attatccaac cctttagagc aaatgttgtc
    20821 gatgtagcac ctcactccat taccattcaa atgacaggtg atggagataa gattgacgcc
    20881 ctcctgcgtg tgattcagcc ttatggcatc aagaatatcg caagaacggg tgcgactggc
    20941 tttagtagag actaatcctt ttcgataata agacctatcc tgcgttgatt tggcttgatg
    21001 gacgttagtt ctatcttcgc ctacatcgcc ta
//
LOCUS       DBNFCK010000024        20350 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.024, whole genome shotgun sequence.
ACCESSION   DBNFCK010000024 DBNFCK010000000
VERSION     DBNFCK010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 20350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20350
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.024"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(62..334)
                     /locus_tag="AC1PIW_000690"
     CDS             complement(62..334)
                     /locus_tag="AC1PIW_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HIP6605646.1"
                     /translation="MFEKKNRTCLTVYLHYNRDARKLSQYGDIVYHSKRLRYVLVYMD
                     QELVEATILKLKKERFVKKVVPSYIKELDQNFVGNLWRDEEPSVVG"
     gene            complement(461..1072)
                     /locus_tag="AC1PIW_000691"
     CDS             complement(461..1072)
                     /locus_tag="AC1PIW_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HIP6605647.1"
                     /translation="MSKRSNFMIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDN
                     MANSIIAQLLFLDAQDPTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGTAA
                     SMGTIIASSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNKLEKI
                     LADNSGKTVKQIHKDAERDYWMSAEETLAYGFIDQIMDNTKVK"
     gene            complement(1168..1797)
                     /gene="upp"
                     /locus_tag="AC1PIW_000692"
     CDS             complement(1168..1797)
                     /gene="upp"
                     /locus_tag="AC1PIW_000692"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HIP6605648.1"
                     /translation="MGKFQVISHPLIQHKLSILRRTSTSTKDFRELVNEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQIAGKKLAIVPILRAGVGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRHIFVVDPMLATGGSAILAVDSLKKRGASNIKFVAL
                     VSAPEGVKALQDAHPDIDIYTAALDEKLNEKGYIVPGLGDAGDRLFGTK"
     gene            complement(1877..3493)
                     /locus_tag="AC1PIW_000693"
     CDS             complement(1877..3493)
                     /locus_tag="AC1PIW_000693"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA];
                     GO_function: GO:0004574 - oligo-1,6-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009311 - oligosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HIP6605649.1"
                     /translation="MPEIQKTDWWKKSVIYQIYPRSFQDSNGDGVGDIRGIISRLDYL
                     HELGIDAIWLSPVYQSPMDDNGYDISDYQGIAPEFGTMEDMEELIAEGHKRNIKIIMD
                     LVLNHTSDEHFWFQEALKGPDNPYYDYYVWADEPNELRSTFSGSAWEYVPHLNKYYLH
                     QFSVKQPDLNWKNPALKQEIWDMINFWIEKGVGGFRLDVIDLIGKEPEKLITADGPTL
                     HPLIQELNEKTFGAYDLVTVGETWSANPENAQLYSHPDRKEFSMIFQFEHVGLDQQEG
                     KEKWDLAPLDPARLHKVLSKWQTELVGKGWNSLFWNNHDLPRAISRFGDDRPAFRELS
                     GKMLAIYLHFMSGTPYIYQGEEIGMINTPITDISQADDIETRRMYAERIENGFTKEEL
                     ITSINAKGRDNARRPMQWTEAEGAGFSTGQAWLALGDTVKDINVEKALADKQSLFYTY
                     QKLIALRKEYPCMSEGKYEVMETGNSSVMAYRKYDEQDDFIILVNFSSQEQAYTIAVE
                     EYSIFMNNYENEEAINKGMLRPFEAIVFKK"
     gene            complement(3566..5014)
                     /gene="gtfA"
                     /locus_tag="AC1PIW_000694"
     CDS             complement(3566..5014)
                     /gene="gtfA"
                     /locus_tag="AC1PIW_000694"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HIP6605650.1"
                     /translation="MKIKNQAMLITYSDSLGKNLKDLKKVLEGPLKDVVGGIHILPFF
                     PSSGDRGFAPMDYTKVDPAFGDWSDIEALSKDYYLMFDFMINHISAKSPYFLDFLEKK
                     DESAYADLFIRYKNFWPNGEPTQEDVDLIYKRKPRAPYRIATFADGSEEKVWCTFDEQ
                     QIDLDVTTETTRRFIQENLEQLAEHGASIIRLDAFAYANKKIGTNCFFVEPDIWEMLH
                     FPRQLLAPKDVEILPEIHEHYTIQQKIAEQDYYVYDFALPMLVLHALYSGHVNRLVHW
                     MEICPRKQFTTLDTHDGIGVVDVKDLLTDEETEETREALYAQGANVKKIYSTEAYNNL
                     DIYQINCTYYSALGNNDQAYLLARVLQCFAPGIPQIYYVGLLAGENDIELLESSKEGR
                     NINRHYYDLEEIEQEVQRPVVQSLFKLLKFRNTSPAFDGEFSVKMLDETSMEIFWNNQ
                     DAGVSARLTANLKEKSFEIVEIEEGKITTIEL"
     gene            complement(5085..5915)
                     /locus_tag="AC1PIW_000695"
     CDS             complement(5085..5915)
                     /locus_tag="AC1PIW_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIP6605651.1"
                     /translation="MNTQKQKNWWVYLVLFSGILFMFIPLLVTIISSFKPTKEITSNF
                     FGLPENFTLNNYERLFNDGIVQYFGNSALITIVAVGLILLVIPMAAFAVARQMKRQTV
                     FNFIYFFLIIGIFVPFQVIMLPMTKLMSSLGLNNIVGLIILYLTYAVPQALFLYVGYI
                     KTIVPEEMDEAAAIDGCDKFTMYWKIIFPLMKPMHATVLIINALWVWNDFLLPLLVLN
                     RDQSMWTLPLFQYNYQGMYFSDYGPSFASYVVGIIPILLVYLIFQKHIISGMTSGSVK
                     "
     gene            complement(5926..6798)
                     /locus_tag="AC1PIW_000696"
     CDS             complement(5926..6798)
                     /locus_tag="AC1PIW_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIP6605652.1"
                     /translation="MNQKGFIAKYWPYLFVAIPIGLQLIFFFYPLFTGIYYSLTDWNG
                     LTSDFKLIGIQNYLDILKNPDFYTSMTFTIIFTIGLVIGEIVIGIWLASLLNRKIKAV
                     GFFRTWYFFPAVLSTVTLGLIFVQLFNYGFTQIGEILHIDWLMENLLVQENTVIPAVL
                     FVALWQGLAMPVIIFLSGLQSIPEDVKEAAAIDGASRSQQFFNIELPFLLPSISMVFI
                     LAMKSGLTAFDLIFALTSGGPDGKTESLGLLVYNYAFVDNKFSYANALAVVLFIFIIV
                     ISLIQMRISKKFEI"
     gene            complement(6933..8168)
                     /locus_tag="AC1PIW_000697"
     CDS             complement(6933..8168)
                     /locus_tag="AC1PIW_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIP6605653.1"
                     /translation="MKMKTFLKCASVCAFASFLVACGNASSSDKVEIEYFSQKPEMQA
                     TLQEIIDDFEKENPTIDVKFSNVPDAGTVLKTRMANNEAPDVINIYPQNADFKAYAAD
                     GRFLEIGDDAGLNHLKDGAVTPYLVNEKNYTLPLTANAYGIYYNKDKFKELGLEVPTT
                     YAEFVALVDKIKADGSAAPFALSLNDAWSLNGYHQLAWVTVAGGFDGAEDILIRSAKG
                     AIQDDATTKAVLERLQLLKDNGQKGATGALYADAVAAFAAGDALMLPQGTWAATAVNQ
                     QEPEFEYGMFTFPGDKEGGDYTIGAADLALSISADTEHPEESKKFLEYLSRPEVIQKY
                     YDVDGSPTSVEGVDTEGKFEETAGVTQYAFTDKHVVWLQSEWESEEEFWNITVEMVKN
                     PNSAELVKKLNAFFDPMKK"
     gene            complement(8181..10355)
                     /locus_tag="AC1PIW_000698"
     CDS             complement(8181..10355)
                     /locus_tag="AC1PIW_000698"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004557 - alpha-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HIP6605654.1"
                     /translation="MNVIEIYNEKQIFHLKTREFSYIIQVLETGDLVHRYFGKKIEKF
                     SDGNKITYLDRSFSPSPITGDRTYSLDVLPLEYSSNGLGDFRTSALDVRNEFGVTLDL
                     KYKEYRLYKGKKELRGLPASFGNQEEVESLEIDLYDQLTDITVTLQYSVFEEASYLAR
                     SATIQTGKYPCKLEKVLSATLDFPHQDFIVHSLAGRYAYEKEWTQTPLTKGQYSIGSI
                     RGASSHSRTPFLALVSPDASEDKGDVYAAHLVYSGNFTAFVETTAMETSRLGLGLESH
                     YFSWQLDKDDRFQTPEVLLSYTDKGFTGMTQNSHHFITKHLIRSSFVNKPRPILINNW
                     EATYFEFTEEKILQLAQVASRAGIELFVLDDGWFGKRNNDESSLGDWKVNLDKLPNGL
                     NGLAERINELGMKFGLWFEPEMISIDSDLYREHPDWAIRTEGRLPIYSREQLVLDLTK
                     QEVCDYIIDSVSSILESANISYVKWDMNRNITNIPEGLANDQRFEFHHRYMLGLYRVL
                     DHLTKRFPDILFESCAGGGGRNDLGIMYYMPQAWASDDTDAIERLSIQEGTSLIYPPS
                     SIGAHVSAVPNHQVGRITPLATRGNVAMMGGAFGYELDLTKLSEKELDEISQQIETYH
                     SIRETIQFGQLYRLKKTSNTWAANYVSQDKNQVVFTFVKILAKPEAPLLHVRLKGLDP
                     DALYECPQLGETFYGDELMNIGLTMPHVQKDYFSVQYIFNKI"
     gene            10460..11299
                     /locus_tag="AC1PIW_000699"
     CDS             10460..11299
                     /locus_tag="AC1PIW_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIP6605655.1"
                     /translation="MNVLNIYNELDSHNFDLNVDHYGAEQCDKGYSFGPTIRDNFVLH
                     FITKGRGKILVDGKTSYLAAGDLFILPKDVSIFYQADDIDPWTYVWVGFSGSRAKDLL
                     NQSQLLENYYLHSNLESPILGYMHQINHVQMHPIPSITELVLIGYLNQLLAALIEEFP
                     SETLIKPETQTKHYVQQAIKMIHNHYAHPIKVSEIADFLALSRSYLYKIFKQETGYSI
                     KDYILQVKMNRSCQLLEDASRSITEIAYSVGYQDPLTFSAAFKNYFHISPTEFRKTQK
                     NKD"
     gene            complement(11331..12491)
                     /locus_tag="AC1PIW_000700"
     CDS             complement(11331..12491)
                     /locus_tag="AC1PIW_000700"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HIP6605656.1"
                     /translation="MTQYNFIDSPNRLSQHAEKWRQVETDKELLPLWIADMDFEPLPE
                     IRQVIRDYAVHHVFGYPYASDSLYQSIIDWEDRQHGYKIERESILLIEGVVPALSVAI
                     QSLTEEGDAVLINTPVYPPFARTVKLNNRQLVTNSLVDVDSIFRLNLDQLEKDIVENQ
                     VKLYIFCNPHNPGGRVWTREEVLAIGRLCQKHGVILVSDEIHQDLTLYGHKHYSFNTV
                     DESFKEFSIILSSATKTFNIAGTKNSFAIIENPSIRKTFAKRQLINNQHEIPTIGLLT
                     TEAAFTYGDEWLKELKVILERNIDFVEEYLTTHTRIKVMKPQGTYLIWLDFSAYELEY
                     AELFDILHCKAKLILNDGLTFGKEGKHHARLNVAAPFNMIEEACQRLGKVFG"
     gene            complement(12495..13586)
                     /locus_tag="AC1PIW_000701"
     CDS             complement(12495..13586)
                     /locus_tag="AC1PIW_000701"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HIP6605657.1"
                     /translation="MTDYKIDTILAHTGINSDERTGALISPIHLSTTYQHPEFGQSTG
                     YDYTRTKNPTRASLETTLAAIEKADYALATSSGMAALVLLFNGFPVGSQVVAARDLYG
                     GSFRWFNEQESIGRFQFTYANTEEELIAAITEETDYVYLETPTNPLMVEFDIAKVSAI
                     AHAKGVKVIVDNTFYSPIYQNPLVLGADVVLHSATKYLSGHNDVLAGALMTNDQALYD
                     KLFYDQNTSGPTLSPLDSYLLMRGLKTLSLRMERATQNAQKIVAYLEKSPAVKQVYYT
                     GKGGMISVKVVNESRIPHILNTLKVFTFAESLGGVESLITYPATQTHADIPAETRHSY
                     GLTDDLLRLSIGIEDAEDLIADLKTALEG"
     gene            13788..14054
                     /locus_tag="AC1PIW_000702"
     CDS             13788..14054
                     /locus_tag="AC1PIW_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605658.1"
                     /translation="MKFQFNKHFYRYSAFYSMIFWTCFFLSDILLDPSQTPAWYSLLA
                     CLIIFINAIWVFFFKEEKELTRMGYIFYIGLIVVIVYLRFILGK"
     gene            14068..14322
                     /locus_tag="AC1PIW_000703"
     CDS             14068..14322
                     /locus_tag="AC1PIW_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605659.1"
                     /translation="MSKKFNKAFYRTWEFYLSLACILQVIYAAVNGPFWFIHFLKLGV
                     GLYAGYLAFFKSRSWIKKTWQIYLTIGLIASWIIISFFVL"
     gene            14332..14583
                     /locus_tag="AC1PIW_000704"
     CDS             14332..14583
                     /locus_tag="AC1PIW_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605660.1"
                     /translation="MFKEFNKEYYRSLNFYLMILLFLGSIGEWLNKGPSLWLALLLFA
                     TVGTARSAFWSKEWTLKANWQIYLCIAFLLVGAWVEFFV"
     gene            14811..15560
                     /locus_tag="AC1PIW_000705"
     CDS             14811..15560
                     /locus_tag="AC1PIW_000705"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HIP6605661.1"
                     /translation="MQKIDLLDGERIDQLFSTDVQIIQNREVFSYSIDSVLLSRFPKM
                     PAQKGLIVDLCSGNGAVGLFASTRTKAQIIQVELQERLANMNRRSIALNGLEDQVSVI
                     NDDLANLPQYDLRSKVDLMLCNPPYFKVDKESNLNESEHYLLARHEIATNLDSICQVA
                     QQVLKSNGRLAMVHRPDRFLDILDTLRTYKLAPKRIQFVYPKASKEANMLLIEAIKDG
                     SVDGLHILPPLVVHEENGDYTPAIREIYYGK"
     gene            15550..15819
                     /locus_tag="AC1PIW_000706"
     CDS             15550..15819
                     /locus_tag="AC1PIW_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIP6605662.1"
                     /translation="MENKAYLYVLECADGSLYTGYTTDVEKRLATHNRGKGAKYTRAR
                     LPVSLLYQEDFDSKQEAMSAEALFKKRSRQSKLDYIAERKQENAK"
     gene            15809..16177
                     /locus_tag="AC1PIW_000707"
     CDS             15809..16177
                     /locus_tag="AC1PIW_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIP6605663.1"
                     /translation="MQNKKHIHRLRIIWHILRVTGFATFLTSFFSFIFLAAGLLLWLE
                     PDFTSYGDSLWYSFVTATTVGYGDMLVTTAFGRIISIILAIYGILFLGSLSGLVVGYY
                     TEVSKHYSLAIKQDKQKTGS"
     gene            complement(16189..16800)
                     /locus_tag="AC1PIW_000708"
     CDS             complement(16189..16800)
                     /locus_tag="AC1PIW_000708"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0050446 - azobenzene reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HIP6605664.1"
                     /translation="MANLLIVKAHPLDAQKSYALRALEEFQTRYASLHPEDRIEIVDV
                     FEDQIPALDKPLLEAMGAAKRGEAISPEQAEQLGRYNALTQQFLAADKIVVVNPLWNL
                     NVPSQLVSWINTINVAGLTFKYGPEGSIGLVKGKKLLHIQSNGGVYAGQDPAAQYIKS
                     IFEFLGFEDIHQVFIEGQSADPSQSQVIFEEAMAKIDQILESY"
     gene            complement(16933..17340)
                     /locus_tag="AC1PIW_000709"
     CDS             complement(16933..17340)
                     /locus_tag="AC1PIW_000709"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6605665.1"
                     /translation="MITYKQNPQLDFQAVLDVYASVGWTGYTSRPEMLEKALEHSLLV
                     LATFDGDRLVGLLRAVGDGHSIVFIQDILVLPTYQRRGIGRQLLEQAVTHFPGIYQLH
                     LLTDNTEKTRSFYEAIGFTAVDSLDCVAYTYLK"
     gene            complement(17452..18054)
                     /locus_tag="AC1PIW_000710"
     CDS             complement(17452..18054)
                     /locus_tag="AC1PIW_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HIP6605666.1"
                     /translation="MKKIDIKTLFATSGIFLLIALLTIYYYGALPDTMVTHFGVNGEP
                     NGSMAKYMVTILTPLLFFCVHLFICVLYDVKGIGKTPVIRVFKWLFPLLALIIQVSLL
                     WYNLGGELDYRRLVIGLLAIGYMVIGNYLPKEELDSKTNEIERKGRKQSGYLFIIGGL
                     LLLVSLLGSPTLSILVLILFGTSVITLSLYYFYRRYKTAS"
     gene            complement(18041..18319)
                     /locus_tag="AC1PIW_000711"
     CDS             complement(18041..18319)
                     /locus_tag="AC1PIW_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HIP6605667.1"
                     /translation="MGLSETLKAISAPIRRQILDSLKSGPKSAGEIVEQFQLTGATVS
                     HHLSTLKKAGLILEEKQKNFIYYRLNYTVFEEVLVWIASFGGQQDEEN"
     gene            complement(18464..20008)
                     /locus_tag="AC1PIW_000712"
     CDS             complement(18464..20008)
                     /locus_tag="AC1PIW_000712"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIP6605668.1"
                     /translation="MKFTDLQLSEDILLAVEKAGFETPSPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLNKIDTNNQAIQSLIIAPTRELAVQSQEELFKFGREKGVKVRS
                     VYGGSSIEKQIKALRSGAHIVVGTPGRLLDLIKRKALKLDGVETLILDEADEMLNMGF
                     LDDIEAIIERVPESRQTLLFSATMPEPIKRIGVKFMKEPEHVKIAAKELTNVNVDQYY
                     LRVKEHEKFDTMTRLMDVDQPELSIVFGRTKRRVDELTRGLKLRGFRAEGIHGDLDQN
                     KRLRVIRDFKNDQIDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     QSGQSITFVSPNEMGYLAIIEDLTKKRMKGLKPATAEEAFEAKKKVALKKIEREMADE
                     TIRNNFDKFKKDAIQLAAEFTPEELALYVLTLTVQDPESLPEVEIAREKPLPFKFAPG
                     QAKGKGGRGGRDRDRGGRRGDRNDRNRRDDKFKRDNRRQDNKKPHQRTSSEKKTGFVI
                     RNKGER"
BASE COUNT         5972 a         4334 c         3834 g         6210 t
ORIGIN      
        1 caaaaaagaa aaaaatttta aaataaaaaa ttagcaggga atacatacct gctaattccg
       61 actatcctac gactgatggt tcttcatctc tccatagatt acctacaaag ttttggtcta
      121 attccttaat gtatgaagga accacttttt ttacaaaacg ttcctttttc aattttaata
      181 tggtagcttc tactagttct tgatccatat acaccaaaac atatcgcaag cgtttggaat
      241 ggtaaacaat atctccatat tggctgagtt tacgtgcatc acgattgtag tgtaaataga
      301 cagttaagca agtgcgattt ttcttctcaa acatgtcttc tcctctaaaa attctctttc
      361 tatattatac cgttttcaat gataaatgaa aagctccaac tacctgtaaa agagttggag
      421 ctaataggaa tagtcagtac tattacatat ttgtcactgc ttatttgact tttgtattgt
      481 ccataatctg gtcaataaat ccgtaagcca aggtttcttc agctgacatc cagtaatcac
      541 gttccgcatc cttgtggatt tgcttgactg tcttacctga gttgtctgcc aagatttttt
      601 ctagcttatt acgtgttttt aataggtgtt ctgcagcaat agccatatct gtttgctgag
      661 taccaccacc agttccaccc attggctggt gaatcatgta ctctgcattt ggcaacatga
      721 aacgtttgcc cttggcaccg cttgatgcaa tgatggttcc catgctcgca gctgttccca
      781 taacgatggt ttgaacatca gctttaatga aattcatcgt gtctacaatg gcaagacctg
      841 ctgacaccga tcctcctggc gtattaacat agaggtaaat atcctttgta gggtcttggg
      901 catcaaggaa aagcaattgt gcaatgatag agtttgccat gttgtcctca actggtcctg
      961 tcaacatgat aatacggtct ttcaaaaggc gtgagtaaat atcataagaa cgctcaccac
     1021 ggctagtttg ttcaataact actggaatca taaaattact ccttttcgag attttctgta
     1081 cccattatat ataaatagtc aaaattggtc aaataaaaaa cacgatttca ggaaatgaaa
     1141 aagaaaccag cagagctgat ttcttaatta ttttgtaccg aacaagcggt cacctgcatc
     1201 tcccaaacct ggtacgatgt agcctttttc attgagtttt tcatccaaag ctgctgtata
     1261 aatatcaatg tctggatgtg catcttgaag agcttttaca ccttctggag ctgaaacaag
     1321 agcaacaaat ttgatatttg aagcaccacg ttttttcaat gaatcaactg caagaatagc
     1381 tgaaccacct gtagcaagca ttggatcaac tacaaaaata tgacgttgat caatgtcttc
     1441 tggtaatttc acaagatact caacgggttg aagggtttct tcatcgcggt acataccgat
     1501 gtgaccaact ttagctgctg gtaccaagct tagcaaacca tcaaccatac caacacctgc
     1561 acgaagaatt ggtacgatag ctaatttttt acctgcaatt tgtttctgaa ctgttttagt
     1621 gattggcgtt tcaatctcca catcttcaag tggcaagtca cgcaaaactt cgtaacccat
     1681 cagcatcgcg atttcattaa ccaactcacg aaaatccttt gttgaagtag atgtacgacg
     1741 aaggatagac aacttatgct gaatgagtgg gtgtgaaatg acttggaatt tccccatgat
     1801 gataaaacct ctcttttttg ttttttctat tttaccaaaa aaattgaaaa aatgcttgag
     1861 aatatggcaa atttatttat tttttgaaaa cgatagcttc aaaaggtctc aacatccctt
     1921 tattaatcgc ctcttcgttt tcataattgt tcatgaaaat agaatattct tccacagcta
     1981 tagtataagc ctgttcctgg cttgaaaagt ttaccaatat gatgaaatca tcctgttcat
     2041 catatttacg gtaggccatt accgagctat ttccagtctc catcacttca tattttcctt
     2101 cagacataca aggatactct ttccgaagag caattaattt ttgataggta taaaaaagtg
     2161 attgtttgtc tgcaagtgct ttttcaacat tgatgtcttt gacagtatct cccaaagcca
     2221 gccaggcttg acctgtactg aagcctgctc cttctgcctc tgtccattgc attggacgac
     2281 gagcattatc tcgacctttg gcatttatag aagtaatcaa ttcctccttg gtgaagccat
     2341 tttcaatccg ttccgcatac atgcgacgtg tttcaatatc gtccgcttgg ctaatatctg
     2401 taattggcgt attaatcata ccaatttcct caccttggta gatataaggt gtccctgaca
     2461 taaaatgaag atagatagcc aacattttac cacttagttc acgaaatgca ggtcgatcat
     2521 caccgaagcg agaaatggca cgaggtaaat cgtggttatt ccagaaaagc gagttccagc
     2581 ctttaccaac cagttcagtc tgccatttgg agagaacctt atgcaaacgt gctggatcaa
     2641 gcggtgccaa atcccacttt tctttccctt cctgctgatc aagtccgaca tgttcaaatt
     2701 ggaaaatcat cgaaaattct tttctgtcag ggtgtgaata taactgagca ttttctggat
     2761 tggcactcca tgtctcccca acagtcacta agtcgtaagc accaaaggtt ttctcattta
     2821 attcctgaat aagaggatga agagtcggtc catcggctgt aattaatttt tctggttctt
     2881 ttccaatcaa atcaatcaca tctagcctga aaccgccgac acctttttca atccaaaaat
     2941 tgatcatgtc ccagatttct tgtttcagag caggattttt ccagttcaga tctggttgct
     3001 taacagagaa ttggtggaga tagtatttgt tcagatgtgg aacgtattcc caagcagagc
     3061 ctgagaaggt cgagcgtaat tcattcggtt catcagccca aacataataa tcataatagg
     3121 gattatctgg tcctttgagg gcttcttgaa accagaagtg ttcatccgag gtatgattga
     3181 gcactaaatc catgattatt ttgatattgc gcttatggcc ttctgcaatc agctcttcca
     3241 tatcttccat ggtcccaaat tctggagcaa ttccttggta gtcactgatg tcgtaaccat
     3301 tgtcatccat gggggactga taaacaggac tgagccaaat agcatcaatt ccaagctcat
     3361 ggagataatc taatcggctg ataatccctc gaatatcccc tactccatct ccatttgaat
     3421 cttgaaaact acgaggataa atttggtaaa tgacggactt cttccaccaa tctgtttttt
     3481 gtatttctgg catttgattg atcctttctg tacaatctta ttaatgtaag aaaaagaggt
     3541 gtccccctct ttctatataa tcatattata actcaattgt ggtaattttt ccctcttcaa
     3601 tttcaacaat ttcaaaggat ttttctttta ggtttgctgt tagacgggca gacacccctg
     3661 catcttgatt attccagaaa atttccatac ttgtttcatc caacatctta acagagaact
     3721 ccccgtcgaa agctggactt gtattgcgga atttaaggag tttgaacaag gattgtacaa
     3781 ctggccgttg cacttcctgc tcaatttctt ccaaatcata gtaatggcga ttgatattgc
     3841 gcccttcctt acttgattct aaaagttcaa tatcattttc gcctgctagt aaaccaacat
     3901 aataaatctg tggaatacct ggtgcaaagc attggagaac acgcgccaat agataagctt
     3961 gatcattatt gccaagagca gaataataag tacagttaat ctgataaata tccaaattat
     4021 tataggcttc tgtactataa attttcttca cattggcacc ttgtgcatac aaggcttcac
     4081 gggtctcctc tgtctcctca tccgtcagca aatccttaac atccacaaca ccgattccat
     4141 catgtgtatc aagagttgta aattgcttgc gagggcaaat ctccatccag tgaaccaggc
     4201 gattgacatg accagaatac aaagcgtgga gaactagcat tggcaaggca aaatcataaa
     4261 cataataatc ttgctcggca attttctgct gaatggtgta gtgttcatga atttctggta
     4321 agatttctac atcttttggt gccaataact gacgtggaaa atggagcatt tcccaaatat
     4381 caggctccac aaagaaacaa ttggttccga tttttttatt ggcataagca aaggcgtcca
     4441 aacgaataat cgaagcacca tgctctgcca actgttccaa attttcttgg atgaaacgac
     4501 gggttgtctc cgttgtcaca tctaaatcaa tctgctgttc atcaaaggta caccacacct
     4561 tctcttcact accatcagca aatgttgcaa ttcgataagg tgcgcgaggt ttacgcttat
     4621 aaatcaaatc aacatcttct tgcgttggct caccatttgg ccaaaagttt ttataacgaa
     4681 taaacaaatc cgcataagct gattcatctt tcttttcaag aaaatcaagg aaataaggag
     4741 attttgctga aatatgattg atcataaaat caaacatgag gtaatagtct ttgcttagtg
     4801 cttcaatatc tgaccaatct ccaaatgcag ggtccacctt tgtataatcc attggcgcaa
     4861 acccacggtc acctgatgat gggaaaaacg gcagaatatg gatacctccc acaacatcct
     4921 ttaacggccc ctcaagaact tttttcaaat ccttaagatt cttgcctaaa ctatccgaat
     4981 aggtaattaa catagcttga tttttaattt tcatagactt tctcctttgt aagccttttc
     5041 ttttttgtta ttttataaaa ggttgaacta gtcaacctta gtaattattt gacagaacca
     5101 cttgtcatac ctgaaataat atgtttttgg aagatgaggt aaacaagtaa gattgggata
     5161 attcctacaa cataggatgc aaatgatggg ccatagtcac taaaatacat accttggtag
     5221 ttgtattgga aaagaggtaa agtccacatg ctttgatcac ggttcaatac tagcaaaggc
     5281 aagaggaaat cattccagac ccagagggca ttgataatca aaacagttgc atgcatgggt
     5341 ttcatgagtg ggaaaatgat tttccagtac atggtaaatt tatcacagcc atcaatagct
     5401 gccgcttcat ccatttcttc aggaacaatg gtcttaatat agccgacgta gaggaagagg
     5461 gcctgaggaa ctgcataggt caaatataga ataatcaaac cgacaatatt gttcaaacca
     5521 aggcttgaca ttaatttggt cattgggagc ataatcactt ggaaaggtac aaaaatccca
     5581 ataatcaaga aaaagtagat aaagttaaac actgtctggc gtttcatttg acgggctacg
     5641 gcgaaagctg ccattggaat aacaagtaga atcaagccaa cagctacaat cgtaatcaag
     5701 gcagaattgc caaaatattg tacaattcca tcattaaaca agcgttcgta gttatttaag
     5761 gtgaaatttt caggaagacc aaagaaattg ctggtaattt ccttggttgg tttgaatgag
     5821 ctgataatgg tcacgagtag cggaataaac atgaacaata tcccactaaa gagaactaag
     5881 taaacccacc aatttttctg tttttgagta ttcatgtctg tcctcctaaa tttcaaattt
     5941 cttcgaaatt ctcatctgaa tcaatgagat gacaataatg aagatgaaga gtactacagc
     6001 caaagcattg gcataagaga atttgttgtc tacaaaggca tagttgtaaa cgagtaaacc
     6061 caaggattct gttttgccgt ctgggccacc acttgtcagt gcaaagataa ggtcaaatgc
     6121 tgtcaaacct gacttcattg ccaagatgaa aaccatactg atagatggta ataagaatgg
     6181 aagctcaatg ttgaagaact gttgagaacg acttgcacca tcaatagctg ctgcttcctt
     6241 cacatcttct ggaatacttt gtaagccaga caggaagatg atgactggca tagccaatcc
     6301 ttgccagaga gccacaaaaa gtacagctgg aataacagta ttttcttgta ccaataagtt
     6361 ttccatcaac caatcgatat gaaggatttc tccaatctgt gtaaaaccat agttgaacaa
     6421 ttggacaaaa atcaatccta aagtgactgt ggatagcact gctgggaaga aataccaagt
     6481 tctaaagaaa ccaactgctt taatcttacg attgaggaga ctagccaacc aaatccctat
     6541 aacaatttct ccgatgacca aaccaatggt gaagataatg gtaaaagtca tggaagtata
     6601 aaaatcagga ttttttaaaa tatctaagta attctgaata ccgattagtt tgaaatcaga
     6661 tgtcaatcca ttccaatccg tcaagctata atagatacct gtaaatagag gatagaagaa
     6721 aaagattaac tgtaagccaa ttggtatggc aacaaataga tagggccaat atttggcaat
     6781 aaatcctttt tgattcatca tggtactcct ttttgataaa gttatagttg cttagtctca
     6841 gttcaaggaa tcccataggg ttactaaaac tgacactagg caactataag gaggctaggc
     6901 tacaactagc ccctgcttat agttttcctg gattattttt tcattggatc aaagaatgcg
     6961 ttcagttttt tcactaattc tgctgagtta ggatttttaa ccatttcaac agtgatgttc
     7021 cagaactcct cttctgattc ccattcagat tgcaaccaaa ctacgtgttt gtcagtgaag
     7081 gcatattgtg tcactccagc agtttcttca aacttacctt ctgtatctac accttcaact
     7141 gaagttggtg agccatctac atcatagtat ttctggataa cttctggacg agagaggtat
     7201 tctaggaatt ttttagactc ttctgggtgc tctgtatctg ctgaaataga tagtgcaaga
     7261 tcagctgcac cgatggtata gtcgccacct tctttatcac cagggaatgt aaacatacca
     7321 tattcaaatt ctggttcttg ttggtttaca gctgtagctg cccatgtacc ttgtggaagc
     7381 atgagagcat cacccgctgc aaaggctgcc accgcatctg catagagcgc gcctgtcgcc
     7441 cctttttgtc cattgtcttt caacaattgc aagcgttcta aaactgcttt tgttgtagca
     7501 tcatcttgaa tcgctccctt tgcactacga atcagaatat cttctgcacc atcaaatcca
     7561 cccgcaacag ttacccaagc taactgatgg tagccgttca atgaccaagc atcgttcaat
     7621 gaaagagcaa acggtgctgc actgccatca gccttgattt tgtctaccaa agccacaaat
     7681 tctgcatagg tagttggaac ttcgagcccc aattctttga acttatcctt attatagtaa
     7741 attccgtagg cattagctgt caaaggaagg gtataatttt tttcgttaac aagatatggt
     7801 gttacagcac catctttcaa atgatttaat cctgcatcat caccaatttc taggaaacga
     7861 ccatccgctg cgtatgcttt gaagtccgca ttttgtggat aaatgttgat gacatctggc
     7921 gcttcgttat ttgccatacg ggttttcaga acagttcctg catctggtac atttgaaaat
     7981 ttaacatcaa tcgtaggatt ttccttttcg aaatcatcaa taatttcctg aagggtagct
     8041 tgcatttctg gtttttgtga aaaatattct atttctacct tatcgctact acttgcattc
     8101 ccacaagcaa ccaaaaagct ggcaaaagca caaaccgatg cacattttaa aaatgttttc
     8161 attttcatta taaaatcctc ctagattttg ttaaaaatat attgtacact aaaataatct
     8221 ttttgaacat gtggcattgt aagaccaatg ttcatcagtt catccccgta gaatgtctct
     8281 cctaattgtg ggcattcata caaggcatct gggtctaacc ccttcaaccg aacatgaagc
     8341 aaaggcgctt ctggcttggc aagaattttt acaaatgtaa agaccacttg attcttatct
     8401 tgactaacat aattggcagc ccaggtattg ctcgtctttt tcagacggta gagctgaccg
     8461 aattgtatag tttcacgaat ggagtgatag gtttcgattt gctgactgat ttcatccaat
     8521 tccttttccg aaagtttcgt taaatccagc tcgtaaccaa atgctcctcc catcatagcc
     8581 acattgcctc gtgtagcaag aggtgtgatc cgaccaacct gatggtttgg aacggcagaa
     8641 acgtgtgcac caattgagga agggggataa atcaaactag taccttcttg aatagataag
     8701 cgctcaatcg catctgtatc atcacttgcc caagcttgcg gcatataata catgattccc
     8761 aaatcattac gacctccacc gcctgcacag gattcgaaaa gaatatccgg gaaccgcttt
     8821 gtaaggtggt ctaatacacg gtagagacct agcatgtaac gatggtggaa ctcaaagcgc
     8881 tggtcatttg ctaaaccttc aggaatattg gtgatattac ggttcatatc ccattttaca
     8941 taagaaatat tggctgattc taatatggaa gagacgctat caatgatgta gtcacagact
     9001 tcctgcttgg tcaaatccaa aactaattgt tctcggctgt agattggtaa gcgtccttct
     9061 gtccgaattg cccaatcagg atgctcacga taaagatcgc tatcaataga aatcatctct
     9121 ggttcaaacc acaatccgaa tttcatacca agctcattta ttcgctctgc taaaccattc
     9181 aaaccatttg gcaacttatc caaattgact ttccaatcgc ccaatgaact ttcatcattg
     9241 tttcgtttac caaaccaacc atcatccaga acaaacaatt caataccagc tcgacttgca
     9301 acctgagcta actgtaaaat cttttcttct gtaaattcaa aataggtcgc ttcccagttg
     9361 ttgattaaaa tgggtcttgg tttgtttaca aacgatgatc gaatgaggtg ttttgtgata
     9421 aagtgatgac tattttgtgt catgccagta aatcctttat cagtatatga taagagaact
     9481 tctggagttt gaaaccggtc atccttatcc aattgccatg agaaatagtg actttccaat
     9541 cctagtccca aacgactggt ttccatggct gtcgtttcga caaatgctgt aaagttgccg
     9601 ctataaacaa ggtgggctgc ataaacatcg cccttgtcct cacttgcatc gggtgaaact
     9661 agtgctaaaa agggtgttct tgagtgactt gaagcacctc gaatgctacc aatcgaatac
     9721 tgaccttttg tcaatggggt ctgtgtccac tctttttcat aagcataacg tcctgccaag
     9781 ctgtgaacaa tgaaatcctg atgtggaaaa tcaagcgttg cagacaagac cttctctagt
     9841 ttgcatggat atttgccagt ttgaattgta gctgaccgtg cgagatagga agcttcttca
     9901 aaaacagaat actgaagagt tacggtaata tctgtcaact gatcgtaaag atcaatttca
     9961 agactctcta cctcttcttg atttccaaaa cttgctggta atcccctcag ttcttttttc
    10021 cccttgtata atcgatactc tttgtatttt aggtcaagag tgactccaaa ttcattccga
    10081 acatctaatg ctgaggttct aaaatctccc agaccattgc tggaatattc gagtgggagg
    10141 acatccaatg agtatgtcct atccccagta attggactcg gagaaaacga tcgatctaaa
    10201 tatgttattt tgtttccatc gctaaatttt tcgatttttt tcccaaaata acgatgaacc
    10261 agatcgcctg tttccaatac ttggatgata taagagaatt ctcttgtttt caggtgaaaa
    10321 atctgttttt cattataaat ctcaatcaca ttcatctctc ctatctcttc tttatggtct
    10381 tattgtacaa aatgaaaacg ctttatttaa tggtcttttg tatttaaaac atgttataat
    10441 gtttcctagg aggattgaaa tgaatgttct aaatatctat aatgaattgg atagtcataa
    10501 ttttgaccta aatgtagacc attacggtgc cgaacaatgt gacaagggct attccttcgg
    10561 tcctactatc cgtgataatt ttgtcctaca ttttattaca aaaggaagag gaaaaattct
    10621 tgttgacgga aaaacaagct atcttgctgc aggtgatttg tttattctgc caaaggatgt
    10681 ttccattttc tatcaagctg atgatataga cccgtggacc tatgtatggg ttggttttag
    10741 tggttcacga gcaaaagacc tacttaatca aagtcaactg ctagaaaact actacctcca
    10801 ctccaatcta gagtcaccaa ttctaggcta catgcatcag attaaccatg ttcagatgca
    10861 tcccatcccc tctatcacag aattggtctt aattggctac ctcaaccaac tcttagcagc
    10921 actcatagag gaatttccca gtgaaacatt aattaagcct gaaacacaaa ccaagcacta
    10981 tgttcagcaa gccatcaaga tgatccataa ccactatgcc catccaatta aagtttctga
    11041 aatagcagat tttctagctt taagccgaag ctatctctat aaaattttta agcaggaaac
    11101 tggctattct atcaaagatt atattttaca agtaaaaatg aatcgttctt gccaactatt
    11161 agaagatgct tctcggagta tcactgaaat cgcctattct gtcggatacc aagaccctct
    11221 caccttcagc gcggccttca aaaattattt ccatataagc ccgacagaat ttagaaaaac
    11281 acaaaaaaat aaggattagg tgattttact cacacctagt ccttatttct ttatccaaaa
    11341 accttaccaa gtcgctgaca ggcttcttca atcatgttga atggtgctgc tacattcaga
    11401 cgtgcatggt gcttaccttc cttaccaaag gtcaaaccat cattcaaaat caacttcgct
    11461 ttacaatgaa gaatatcaaa gagctccgca tactctaatt cataggcaga aaaatcaagc
    11521 cagatgaggt aggttccctg aggcttcatg accttgatcc ttgtatgggt tgtcaaatac
    11581 tcctcaacaa agtctatatt tctctccaaa ataaccttca actccttcaa ccactcatct
    11641 ccataagtga aagcagcttc tgtggttaac aagccaatcg ttggaatctc gtgctgatta
    11701 tttatcaatt gacgcttagc gaaagtcttg cgaatacttg gattttcaat gattgcaaag
    11761 ctattttttg taccagcgat attgaaggtc ttggtcgcag atgataagat gattgaaaac
    11821 tctttaaaac tttcatccac tgtgttaaag gaataatgct tgtgaccata caaggtcaaa
    11881 tcttgatgta tttcatctga gacaaggata acaccatgtt tctgacaaag acgtccaatg
    11941 gccaacactt cctctcttgt ccaaactcta ccacctgggt tgtggggatt acagaaaata
    12001 tagagtttca cttgattctc tacaatgtcc ttttcaagct ggtcaagatt aagcctaaaa
    12061 atactatcta catcaactag tgaattggtc accaactgac gattgtttaa ttttacagtc
    12121 cgcgcaaagg gtggatagac aggtgtatta attaggactg catccccttc ttctgtcaaa
    12181 gattgaatag ccacagataa ggcaggaaca actccttcaa tgagtagaat agactctctt
    12241 tcaatcttat agccatgctg tctatcttcc cagtcaataa tagactgata gaggctgtca
    12301 ctagcatagg gataaccaaa aacgtgatga actgcgtagt cacggataac ttggcgaatt
    12361 tctggtagag gctcaaagtc catatctgct atccacaagg gtaagagttc tttatccgtc
    12421 tctacttgtc tccatttttc cgcatgctgg gatagacgat tcggactatc tataaaatta
    12481 tactgtgtca tatcttatcc ctccaaggct gtctttaaat ctgcaatcaa gtcttccgca
    12541 tcctctatac caatagacag acgaagcaaa tcatctgtca aaccgtaaga atgacgggtt
    12601 tcagctggaa tgtctgcgtg ggtctgagtg gctggatagg taatcagact ctctactcca
    12661 cccagacttt cagcaaaggt aaagactttc agggtgttga gaatatgggg aatccgactt
    12721 tcatttacca ccttaaccga aatcatccca ccttttcctg tgtagtaaac ttgcttgact
    12781 gctggactct tctccaaata ggcaacaatt ttctgagcat tttgtgtcgc ccgctccatt
    12841 cgaagcgaga gggttttgag tccacgcatg agtaagtaag aatccagtgg agatagggtt
    12901 ggacccgatg tattttggtc ataaaagagc ttatcatata gggcttgatc attggtcatc
    12961 aaagcgccag ctagtacatc attatgccca gataggtact tggttgctga atgtaggaca
    13021 acatccgctc ctagaacaag tggattttga taaatagggc tgtaaaaagt gttatcgaca
    13081 atcaccttaa cacctttggc atgggcgata gccgaaacct tagcgatgtc aaactccacc
    13141 atgagtgggt tagtcggtgt ctccagataa acatagtctg tctcttcggt gatggctgca
    13201 atcagctcct cctcggtgtt tgcataggta aattgaaatc taccgatact ctcttgctca
    13261 ttgaaccagc ggaaggaacc accgtacaaa tcacgcgccg ctaccacttg actccctaca
    13321 ggaaaaccat taaaaagtaa gaccagagca gccattcccg agctcgttgc tagggcatag
    13381 tcggcctttt caatagctgc caaagtcgtc tctagactgg cacgtgtcgg attttttgtt
    13441 ctcgtgtagt cataacctgt cgattgacca aactcagggt gttgataggt cgtcgaaagg
    13501 tggatagggg aaatcaaggc acctgttctc tcatcgctat tgatgcccgt atgcgctaaa
    13561 atcgtatcaa tcttataatc tgtcataaca tctactccaa tctaacttac aatatagtct
    13621 agcatgaaat aggagatttg gtgtgaaaaa tatccaaaaa tacttataga aaaatagcaa
    13681 gctttgttat agtttttctt tataacaata tccatactgg atctaccaat aaatactttt
    13741 aaacctataa tttatttgat ataataaaag aaagagaggt gattgtcatg aaattccagt
    13801 ttaacaagca tttctatcgg tatagtgcct tctattctat gattttttgg acttgttttt
    13861 ttctgtcaga cattttgcta gacccttccc aaactcctgc atggtatagc ctactagcct
    13921 gtctgattat ttttatcaat gccatttggg tattcttctt caaagaagaa aaggagctca
    13981 ctcgcatggg ttacatcttc tatatcggat tgattgtagt gattgtttat ctgagattta
    14041 ttttaggtaa gtagaaagga gtttactatg tctaagaaat tcaataaagc attctatcgg
    14101 acctgggaat tttacttgtc tcttgcctgt atcctacaag tgatttatgc tgctgtaaac
    14161 ggaccgtttt ggtttatcca ttttctcaag cttggagtcg gactttatgc tggttatcta
    14221 gctttcttta agtcacgtag ctggataaaa aaaacatggc aaatttatct gacgattgga
    14281 ttgattgcta gttggataat catttccttc tttgtattgt gaggatttta tatgtttaag
    14341 gaatttaata aagaatatta tcggagtttg aatttttatc tgatgatatt gttgtttcta
    14401 ggaagtatcg gggagtggct aaataaggga cctagcctct ggcttgcact cttgctcttt
    14461 gctactgttg gtactgcccg tagtgccttt tggtctaaag aatggacctt aaaagcaaat
    14521 tggcaaatct atctctgtat cgccttcttg cttgttggag cttgggtaga attttttgtt
    14581 taagagttgg gagtgggaaa gaactcgact aagtcaaaaa gagttcgtat tcccaccccc
    14641 gcacagttga ttaggtcaga tttggagtgt aaaacacgaa caaatctgcc aatcaaccac
    14701 tgcgctgaga tgttgacacg aacactgaga agtgggccag ggctttttgc ccagcctctt
    14761 ttctttcaaa ataaacagtg attttcattc aaagatggta taatatacct atgcaaaaaa
    14821 ttgatttact agacggagag cggattgacc agctcttctc gacagatgtt cagattattc
    14881 aaaatcggga ggtatttagc tactcgattg acagcgttct tctttcgcgg tttccaaaaa
    14941 tgcctgctca gaaggggttg attgttgacc tttgtagtgg aaatggggcg gtgggtctct
    15001 ttgcttctac acgcaccaag gctcagatta ttcaggtcga gttgcaggaa cgcttggcga
    15061 atatgaaccg ccgctccatt gccctcaacg gtctggagga tcaggtttcg gttatcaatg
    15121 acgacttggc aaatctaccc caatatgacc tacggtccaa ggttgatttg atgctctgca
    15181 atcctcccta cttcaaggtg gataaagagt caaacctcaa tgaaagcgag cattatctcc
    15241 tagccagaca tgagattgca accaacctag acagcatttg tcaggtggcc caacaggtgc
    15301 tcaagtccaa tggtcgcttg gccatggttc acagacccga ccgattttta gatattttag
    15361 acacactaag aacctacaaa ttggctccca aacgcatcca gtttgtctat cccaaggcca
    15421 gcaaggaggc caatatgttg ctgattgagg ctatcaagga cggctcggtg gacgggctgc
    15481 acatcctgcc acctctggtt gtccatgagg aaaacggcga ctatacacct gccattcgtg
    15541 agatttatta tggaaaataa ggcctacttg tatgttttgg aatgtgccga cggaagtctt
    15601 tatactggtt atacgactga tgtggagaag cgacttgcta cccataatcg tgggaaaggg
    15661 gccaagtaca cccgcgcacg cttgcctgtc agcctgctct atcaggaaga ctttgacagc
    15721 aaacaggaag ctatgtctgc cgaggctttg tttaagaaac gcagtcggca gagtaaatta
    15781 gattatattg cagaaagaaa acaagaaaat gcaaaataaa aaacatatcc accgccttcg
    15841 catcatttgg catatcttga gagtgactgg ttttgccact tttctcactt cctttttcag
    15901 ttttatcttt ctagcagctg gtttgctact ctggttggag cctgatttta ccagctacgg
    15961 tgatagttta tggtacagct ttgtaacggc gacgactgtt ggctatggag atatgctggt
    16021 caccactgct tttggacgga ttatcagcat tatcttagct atttacggca ttctcttttt
    16081 ggggagcctg tctggattag tcgtcggcta ctataccgag gtcagcaagc attattcttt
    16141 agcaatcaaa caggacaagc aaaaaactgg ctcttaggaa ccagtttttt aatagctttc
    16201 caaaatctga tcaattttag ccattgcctc ttcaaaaata acctgagact gacttggatc
    16261 ggcagattgc ccttcgataa agacctgatg aatgtcttca aatccaagaa actcaaagat
    16321 agacttgata tattgggcag ctggatcttg acctgcataa acaccaccat tggactggat
    16381 gtgcaacaat tttttgccct tgaccagacc gatagagccc tcaggtccat acttgaatgt
    16441 cagtcctgca acgttaatgg tattgatcca tgacactaat tgacttggca cattgaggtt
    16501 ccaaagggga ttgaccacga caatcttatc tgctgcaaga aattgttggg tcaaggcatt
    16561 gtagcggccc aactgctctg cttgttcagg gctgatagct tcgcctcttt tagctgctcc
    16621 catagcctct aacaagggct tatccaaagc tggtatttgg tcctcaaaga catctactat
    16681 ttcaatccga tcttctgggt gcaaactggc gtaccgtgtt tgaaactctt ccaaagcccg
    16741 caaggcgtag gacttttgtg cgtcaagtgg gtgggctttt acgattaata gatttgccat
    16801 tggttctctc ctttgttatc cgttattact aattattgat aaatatattc tactcttttt
    16861 gatttgaaat tcaaatcatt tgctcgaagc aaaaggctgg tctcccagcc tctgcttttt
    16921 tagattgttt tattatttta aataggtgta agcgacacag tccaggctat caacggctgt
    16981 aaagccaatt gcctcataga aagaccgtgt tttctcagta ttgtccgtca agagatgaag
    17041 ctgataaata cccggaaagt gggtaacggc ctgctccaaa agctgacgcc caattcctcg
    17101 tcgctgatag gttggtagga ccaagatgtc ctgaataaaa acgattgaat gcccgtcgcc
    17161 aactgctcgc aagagaccca ctagacggtc accgtcaaag gtagcaagaa cgagcagact
    17221 atgttccaag gccttctcta gcatttctgg acggctagta tatcctgtcc aacctacaga
    17281 agcatagacg tcaaggacag cttgaaaatc gagttgagga ttttgtttgt aagtaatcat
    17341 gtctttctcc tttgtattgt tttttagaat acaaaggctt tgtccattgt taaaacgccc
    17401 atggtctttc ctcctgtttt gctacattta gtataacaaa aactgctcca attatgaagc
    17461 agttttgtaa cgacgataaa aataatagag actcaaggtt atgacagatg taccaaacaa
    17521 aatcagcaca aggatagata gggttggtga gcctaataga ctaactagca gcaataaccc
    17581 accaataatg aagagatagc ctgactgctt tcttcccttt cgttctattt cattggtctt
    17641 gctatccaat tcctccttgg gtaaataatt ccctattacc atatagccga tagccagcag
    17701 tccaatgaca agcctacggt aatccaattc tccgcctaaa ttataccaga gtaaagaaac
    17761 ttgaatgatg agagccagta gcggaaaaag ccatttgaac actcgaatga ctggtgtttt
    17821 ccctatccct ttcacatcat aaagcacaca aataaatagg tgaacacaaa agaatagtag
    17881 cggtgtcaaa attgtcacca tatacttagc catagagcca tttggctcac catttacgcc
    17941 aaaatgcgtt accatggtat ctggcaatgc tccatagtaa taaatcgtca ataaagcaat
    18001 cagtaggaat ataccagaag ttgcaaataa ggtttttatg tcaattttct tcatcttgtt
    18061 gccctccaaa acttgcaatc caaaccaaga cttcttcaaa gaccgtatag tttagacgat
    18121 aataaataaa atttttctgc ttttcctcca aaattaatcc agcttttttc aaagtggata
    18181 aatgatgaga aactgttgct cctgttaatt gaaattgttc tacaatttcc ccagcagact
    18241 tcggccccga cttaagacta tctagaattt gcctacgaat aggagcagaa attgccttca
    18301 aggtttcaga caatcccata ataagcctcc ttgtccatct atttagaaat atttctaaat
    18361 agaattatac tctgttttta ttgtttgtca agaactattt cgattatttt ctaaatagat
    18421 ttcaaaaaaa acagaccact ctttatggtc tgtcaaatcc ggcttatctc tcccctttat
    18481 tgcgaatcac aaagcctgtt ttcttttcac ttgatgtgcg ttggtgtggt tttttgttgt
    18541 cttgacggcg gttatcgcgc ttgaacttgt catcgcgacg gttgcggtcg ttacgatcac
    18601 cacggcgtcc tccgcgatca cggtcgcgac ctccacgtcc acctttaccc ttggcttgac
    18661 caggtgcaaa cttgaatggt aatggttttt ctcgcgcaat ttctacctct ggcagacttt
    18721 ctggatcctg aacagtcaag gtcaatacat aaagcgctaa ctcttcaggg gtaaattcag
    18781 ccgctaactg aatagcatct ttcttgaact tatcaaaatt gttacggatg gtttcatccg
    18841 ccatttcacg ctcaatcttc ttcaaagcaa cttttttctt ggcttcaaag gcctcttcag
    18901 ctgtcgctgg cttgagccct ttcatgcgtt tcttggtcaa atcttcgata atagccaagt
    18961 agcccatctc gtttggcgaa acaaaggtaa tagactgacc tgattgaccc gcacgacctg
    19021 tacgaccgat acggtgaaca tagctttctg gatcctgtgg aatatcgtag ttatagacat
    19081 gcgttacacc agaaatatcc aaaccacgcg ccgcaacgtc cgtcgcaacg aggacatcaa
    19141 tctggtcatt tttaaagtca cgaataacac gaagacgctt attctgatcc aaatctccgt
    19201 ggataccctc tgcacggaag ccacgcagtt tcaaaccacg agtcaattca tcaacacggc
    19261 gcttagtacg accgaagacg atagacaatt ctggctggtc aacgtccatg aggcgtgtca
    19321 tggtgtcgaa tttttcatgt tctttgacac gaagataata ttggtcaaca ttgacattag
    19381 tcaattcctt tgctgcaatc ttaacgtgct caggctcttt catgaatttg acaccaatac
    19441 gcttgattgg ctctggcatg gttgcagaaa acagaagagt ttgacggctt tctggcacac
    19501 gttcgatgat ggcttcaata tcatccaaga agcccatgtt gagcatttca tcagcctcat
    19561 caagaataag ggtttcaaca ccgtcaagct tgagagcttt acgtttgatc aagtccaaga
    19621 gacgaccagg tgtaccaaca acaatgtgag caccagaacg aagggccttg atttgttttt
    19681 caatgctaga accaccgtaa acagagcgaa cttttactcc tttttcacga ccaaatttga
    19741 aaagctcttc ctgactctgc accgccaact cacgcgttgg ggcgataatc aaggattgaa
    19801 ttgcttgatt gtttgtatca atcttgttga gggttggcaa accaaaggct gcagttttcc
    19861 ctgtccccgt ctgcgcttga ccaatcacat cttttccttc cagagcaagc ggaatggtct
    19921 gctcctggat tggtgacggc gtttcaaaac cagccttctc aacggcaagc aaaatatctt
    19981 ctgataattg taaatctgta aatttcaatg tattctcttt tctaaaaggc agtgcgaagc
    20041 taccttatct aacttttttg taacaagata tcagggtcga accactgata ttggatttgc
    20101 aacttaccaa gtataacaca atctaacaat aatagttaat atttaccttt aaaaagtcaa
    20161 taatacgttt tcatgaggag tccatcattt tatagacatt tgaattagct ttgatacatc
    20221 gatactttcg gtttgcccta ctctagtacc agctccttgt ctgaaagtta tactcttcga
    20281 aaatcaaaat cagacgttgt tgacttgatt tgatgaactt cagttctatc tgcatctgcg
    20341 tcgcctagtc
//
LOCUS       DBNFCK010000025        19652 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.025, whole genome shotgun sequence.
ACCESSION   DBNFCK010000025 DBNFCK010000000
VERSION     DBNFCK010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 19652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19652
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.025"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            133..1068
                     /locus_tag="AC1PIW_000713"
     CDS             133..1068
                     /locus_tag="AC1PIW_000713"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006597158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HIP6605669.1"
                     /translation="MVSTVTTLGGFTFDNCLMNAAGVWCMTKEELDAVKNSKAGTFVT
                     KTATLDYRAGNPEPRYQNVPLGSINSMGLPNQGLAYYLDYLLELQETEPERTFVLSVV
                     GMSPDETHEILRTVEASDFKGLTELNLSCPNVPGKPQIAYDFETTETILQEVFTYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNQFPLKFVNCVNSIGNGLYIKDESVVIKPKNGFGGI
                     GGEYIKPTALANVHAFYQRLKPEIQIVGTGGILTGRDAFEHILCGASMVQVGTTLQKE
                     GVAAFGRITAELQAIMAEKGYETIEDFRGKLQQDD"
     gene            1401..2633
                     /locus_tag="AC1PIW_000714"
     CDS             1401..2633
                     /locus_tag="AC1PIW_000714"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HIP6605670.1"
                     /translation="MERRANYIRRKRYASYRTKMHLFFICGLATIILAIIIVSEHLVP
                     RLNQASESMHSDNSVRIMAHGDLLYHLPILRGAEQPDGSYDFSENFTYVKPWIEQADF
                     AIADFEGTISPDIELAGYPLFNAPAIVANNIHDAGYDLVDLAHNHILDSHLSGLISTV
                     NTFRDAGVDTVGVYAEGNRATAPLYIREVNGIRIAVLAYAYGFNGMEALLSQEEYDAY
                     LSDFDKEKMRAEIERAEKEADITIVMPQTGVEYQLEPTEEQTSLYHQMIDWGADIVLG
                     GHPHVVEPTEILEKDGQRKLIIYSMGNFLSNQRIESMEDTPNAQWTERGVLMDLTIQK
                     ENGQTSIQTAQAHPTWVNRLSKGTYSTDGYEQFSYQTYILEDWVAGGRYYGQLDLDTQ
                     ARVDTAYQEMKDFVNLVW"
     gene            2645..3472
                     /locus_tag="AC1PIW_000715"
     CDS             2645..3472
                     /locus_tag="AC1PIW_000715"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIP6605671.1"
                     /translation="MIVRLIATDMDGTFLDGQGNFDRERFSRVLDQLDQKKIPFVIAS
                     GNGIGRLLQLCQGFEDRLIFVADNGAHVYQNGKTVIRRAIQQVEVEAILHFFKGRWAD
                     VCLMLSNDGNIYMQAGAGMPFAGTDLPIELAQMAAFQNRVTYLDNLSAYPVSESIHKV
                     GLWVPEARVESITEAFNQGFHGQLVAVTSGYGSVDILPQGIHKAWGLEQVLTGLDIEP
                     EQVMAFGDSDNDIELLSYVGYSYAMENATDKVKAVAKYMAPSHLEAGVLQVIEEYIL"
     gene            complement(3521..4303)
                     /locus_tag="AC1PIW_000716"
     CDS             complement(3521..4303)
                     /locus_tag="AC1PIW_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6605672.1"
                     /translation="MKKYISLYLYNIKVYMMAQMSFRVDFFIGLFSSLIEQIVYLIFL
                     NILFGNIKEIAGFNYGQMLFIYGIATVGRSIHLIFFDNLWMFGSRYIRQGEFERLLLM
                     PVNPLFQLICERIQPQGIGTTLIGSLALVQASNELGMEWSLGKLALLIFIAICIGLLY
                     AAIQLGPTALAFWIVESFPLTMGIFALNQMAQYPLNIYPKIIQILLIFVFPYAFTAYF
                     PALYFLDLSMWGLALPLVVVVLFSINYNLFRYGMTKFTSVGN"
     gene            complement(4305..5084)
                     /locus_tag="AC1PIW_000717"
     CDS             complement(4305..5084)
                     /locus_tag="AC1PIW_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6605673.1"
                     /translation="MRKYLYMTRLTMMNAMQYKAFFLATFVSLAVKILVALYVWKTIF
                     FTQSEVNGFTLQTFTTYIIFANLLASLNSFSLGEDLSYSILKGSIAGEFLRPYSFILA
                     LFFKDLGNKLLELIKFAIVFIGILLVHQDFYLPDGKTILLFLVSSILGMFIVQLLDMA
                     FGFLAFFTVNAWGVMLLRMGLFNLASGALLPLSFYPQAVENFLKLLPYNYAVNVPVSI
                     LLGQESDLTALGLQVIWIPILACFIAALWSQAKRRIVIFGG"
     gene            complement(5077..6060)
                     /locus_tag="AC1PIW_000718"
     CDS             complement(5077..6060)
                     /locus_tag="AC1PIW_000718"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6605674.1"
                     /translation="MIQAEHLSKTYSIIDKEVGLKGSIKAFFKPKKKSIPAVQDISLQ
                     VGKGKIIGYIGSNGSGKSTTIKMLTGVLFPDQGQVRINGLNPQENRKAVNKQIGVLFG
                     QKSHLDWNLPVQESFILHAKIYDVPDKVFKERLAVLIELLDLADIMKQPIRNLSLGQR
                     VRCEFAAIFIHQPAVVFLDEPTIGLDASVKETIRSFIRYMNQEYQTTFLITSHDMKDI
                     ESLCERIFIIDKGKKVYDGSLTVLKERFSTVKTILFSTEKPIEKDLQLEGWEFIRKDD
                     FHFEIHYQSKVWTSAQVIEQVFNHYSIEDVTMKELEIETMVRQIYEEGIHA"
     gene            complement(6145..6678)
                     /locus_tag="AC1PIW_000719"
     CDS             complement(6145..6678)
                     /locus_tag="AC1PIW_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIP6605675.1"
                     /translation="MRTDSRKERTRQAILEAMVTCLESQGFNDITTTHLAQTAGISRS
                     SFYTHYKDKYELIDSYQQGLFHKLEAIFDHYDGNRQSSFLEIFELLYREKLLSALLTH
                     NGTQEIQNFLINKVRLLVANDLAERLGKENLSQLEKEYSSIYFSHAFFGIMQVWIKNG
                     KQESPQYMTDFLIKMLP"
     gene            6804..9263
                     /locus_tag="AC1PIW_000720"
     CDS             6804..9263
                     /locus_tag="AC1PIW_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HIP6605676.1"
                     /translation="MLQKVKNIFKKPMTLITILGIACVPALYNISFLTSMWDPYGRLD
                     QLPVAVVNQDQSASFQDKTLTIGDDMVDNMKESKSLDFHFVSEKNAEKGLEEGDYYMV
                     ITLPEDLSEKATSLLTNQPEPITISYQTSKGHSFVASKMGESAMEKLKTSVSETITKT
                     YTTAVFDSMREIQTGMIEAADGSQKLTNGASQLESGSQTLSNGLTTLTTSGQVLVTGA
                     NQLATGVVSYTDGVNQAATGSQTLSSGLTIYTNGVASLVSGAEQLNANSSQLITGVGQ
                     LQSGASQVEQLVTGANQLQAGLEQLANSTSLSAEQSSQIQALLTGLPQLQVAINQLND
                     SLSSIGGLAVDNSTLSSLLTEMGAQAQGLLTAAQADKTASIEALQTTATYQNLTADQQ
                     AELVGALQNSPSATATVAQTILDQLSQLSQTLSSLQSLSGMATQMNQLQSAVGQINTA
                     ANQALPGATTAIESLSSGLSQVNTALNQQVLPGTQTLTSGVSQLQTQLSSGASQLMFG
                     VTAYTAGVAQLAAGGAQLVANNSSIQSGGGQLTSGLATLASNSSQLVSGSEQLASGSQ
                     QLIAGADQLASGGQALTSGISSLRTGSETLTNSLSSASQQLSVVSVEDKNAQAVSQPV
                     TLEHSDQDDVKTNGVGMAPYMVSVALMVAALSANVIFVKHIDNRSYKNRWDWAKGKLL
                     LNGIIASLAAVILYGVLRLIGIEPAHPMATLGIILLASWTFMALVTALVGWNNRFGSF
                     ASLIILLLQLGSSAGTYPIELSPRFFQVIQPYLPMTYSVSSLRQTISMVGNSSHQVWM
                     LSLFLVGFMGLGLLIYNQKDE"
     gene            complement(9313..10371)
                     /locus_tag="AC1PIW_000721"
     CDS             complement(9313..10371)
                     /locus_tag="AC1PIW_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIP6605677.1"
                     /translation="MKKKLTIAIIIGFVSLIAGLILAGIGFFTGGITRLEEVAAPTEV
                     HKTFTDLNTIKIDFIPHSVYIKESSDNNYHVTYANSDNNIQNPLKLSEKDGVLTLSAQ
                     ELEFAIEGIMQYLGESLAQRRINVFSVTIEVPKGKTLDKLEGHNLHFYDFGGFTIENI
                     HIKEANLSGGVTLDKVTIDSGQIEAGYFQATQSTLKDMHINLQGSSVRLYETSLENVT
                     IEGYSQLDSRQTTLLGDNRFTPSDTYSSTTFLDVTDKSLADSNLLITNQIDKKKLVEA
                     QGYYYEDGNDLGEMVNQDPYLKERLGEVGIFTSDKYTKFPVKTENDLQTLTLENKGSK
                     NKLTIEATNATINLGTPK"
     gene            complement(10368..11123)
                     /locus_tag="AC1PIW_000722"
     CDS             complement(10368..11123)
                     /locus_tag="AC1PIW_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HIP6605678.1"
                     /translation="MTRTEYMEQLEKYLKKLPHKEYFEAISFFNEYFDEVGPERETEI
                     IEELGSPKEAASELINNMLNKQIQEEKDQQEPIQLNWKLWVGLGALSMTGLFSFFLLF
                     IMGEFIGVIPLFATLILGAFFLGRYFRNFSQTKRTLWLAILAVISLPIAIPLLLILLA
                     SLLGLVALILALIVGAFVLGVGLLTSGGYLIWEAFSLMSEGFNIFLMGFGSGLSLIGG
                     AILIYILTGFFAYWSWRLVKACFKWILKRGKRA"
     gene            complement(11110..11436)
                     /locus_tag="AC1PIW_000723"
     CDS             complement(11110..11436)
                     /locus_tag="AC1PIW_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIP6605679.1"
                     /translation="MHFPVPAVLTEFLIMAILESDDSYGYEICQTIKLIANIKESALY
                     PILKRLEQNDFLTTYSQEYQGRMRKYYSLTQLGHEELVRLKDDWDTYTNTINGIIEGS
                     VRHDKN"
     gene            11745..12125
                     /locus_tag="AC1PIW_000724"
     CDS             11745..12125
                     /locus_tag="AC1PIW_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HIP6605680.1"
                     /translation="MKLYVQFMIILVFSFLGEAISTIFHLPIPGSIIGLILLFLALEF
                     KLIRLRHIHTVGNFLLANMTILFLPAAVGIMERFDAIKDYLLPIIVVILGAIFLNILV
                     IGFVVQFVKQKFEGDYIDAEGNHD"
     gene            12118..12813
                     /locus_tag="AC1PIW_000725"
     CDS             12118..12813
                     /locus_tag="AC1PIW_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HIP6605681.1"
                     /translation="MTELFSNPIFGIMLSIAAYLIGMLIYRRFPHPITTPLLVATGLI
                     IVFLKMTGISYKEYYAGGSYLNMLIVPSTVALGIPLYRSFHLMKHHIRSILLGIFVAC
                     IVNTVFTALIAKWFGMDFFLAISLFPKSVTTAMAVGITDKMQGIATVTLVVVVATGIL
                     TSVLGPVFLKLLKIEDPVAVGLALGGTGHAIGTGTAIKCGHTQGAMAGLAIGITGIMY
                     VVISPIVAQIILQ"
     gene            complement(12846..13646)
                     /locus_tag="AC1PIW_000726"
     CDS             complement(12846..13646)
                     /locus_tag="AC1PIW_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HIP6605682.1"
                     /translation="MGANQETLMYTLEKSIKKKADLFEHSFSAYAVRSILASLYLGLG
                     IVISLYTADKLNHVAEGLGKFSYGLMFGWGLLMILYMNAELGTSNMMYMTVASHRKTI
                     PTKTALKMLATCILFNFVGAVLVCYLVSLTLPYQHVDAHSYLFEATVAKLSKTPLTQF
                     IEGIFANIVVNIGVFTFLRIKDDAGRLISVIFIIFIFAFLGYEHVIANFSLFSLAFFA
                     NGGPVEGMTLLSVLSNFLFSGLGNYVGGGLFIGLLYSWLNNQSKLYVD"
     gene            13787..14632
                     /gene="gla"
                     /locus_tag="AC1PIW_000727"
     CDS             13787..14632
                     /gene="gla"
                     /locus_tag="AC1PIW_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaglyceroporin Gla"
                     /protein_id="HIP6605683.1"
                     /translation="MDVTWTVKYITEFLATALLIIIGNGTVANVDLKGTKGNNSGWIL
                     IAIGYGLAVMMPALMFGNVSGNHINPAFTLGLAVSGLFPWAHVAQYIVAQLLGAMFGQ
                     LVVVAVYKPYFLKTENSNHILGSFSTISALDNGTKESRKAATTNGFLNEFVGSFVLFF
                     GALALTKHFFGSELVGKLIEQGYDKTVAETMTAPYTSGSIAVAHLGIGFLVMALVAAL
                     GGPTGPGLNPARDLGPRIVHALLPKSVLGENKADSKWWYAWVGVCAPIIASIAAVALF
                     KFLYL"
     gene            14761..16293
                     /locus_tag="AC1PIW_000728"
     CDS             14761..16293
                     /locus_tag="AC1PIW_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HIP6605684.1"
                     /translation="MSDVIAFGYGSSRAKMQLKRLNRHGIIAGATGTGKTVTLKVLAE
                     QLSDAGIPVFLSDIKGDLNSLVAANTKEIDPSRLEKTHYLDYSPVGYPVELWDVLGEN
                     GTPIRMTISELGPVLLTRLLGLNDTQESILNIVFSVADERGLLLIDLMDLRAMLNFVA
                     ENAAELSQYYGNIPARSVGAILRSLVVLEQQGGKIFFGEPSLDIADLMRTAEDGRGVI
                     NVLQATQLFNQPTLYSTVLLSLLSELYEVLPEVGDLDKPKMVFFFDEAHVLFKDAPKV
                     LLEKIELIVRLIRSKGVGVFFVTQNPTDIPDSVAAQLGNRIQHGLRAFTPKELKTVAT
                     VAETFRQEGDEDLAKVIQELQVGEAVVSTLQADGTPSFADRVLIYPPKSMLGTVEPSA
                     LLSVINNSPLMEKYADAVNRESAHEQILAMTEAKEAELIKKAEQAEAEKQAEKEAKAA
                     AKMEEKAQKEAAKTAQKTSQPASRKTDSMMDRFTKNLMSQVGREVGRVVTRGIMGMLK
                     GK"
     gene            16405..17013
                     /locus_tag="AC1PIW_000729"
     CDS             16405..17013
                     /locus_tag="AC1PIW_000729"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIP6605685.1"
                     /translation="MTDQLQALLKDYQPQPLGEKRSYAVFLPLVWSDNQWQVLYEIRS
                     ESISQPGEVSFPGGGVEVGETPQQAAVREVMEELNIQPEQIDILGEIDYLVLECSTVH
                     CFVGRLNLDWTTILPNEEVARIFTVPLSTLLTTQPVYYQLDSQIVPDCDFPFERLRGG
                     VDYPFSHHKRSVPFYENLSENIWGMTAQFTHRFTEIVKSNLS"
     gene            17303..19600
                     /locus_tag="AC1PIW_000730"
     CDS             17303..19600
                     /locus_tag="AC1PIW_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIP6605686.1"
                     /translation="MSKQKVVSSLLLSTVVLGGLAFYSTTTVKAESLEPDVTSVNASD
                     ATNKPVVPNEEGGDFLAEEELEDDDTLEEELNEKAEEVTEPSSPEALLQPRAMMSDSE
                     TSGMEEIPMNDEPSDNTEEKVEKQQSPLIQTSNADYKSGKDQEKLRTSVSINLLKAEE
                     GQIQWKVTFDTSEWSFNVKHGGVYFILPNGLDLTKIVDNNQHDITASFPTDINDYRNS
                     GQEKYRFFSSKQGLDNENGFNSQWNWSAGQANPSETVNSWKSGNRLSKIYFINQITDT
                     TELTYTLTAKVTEPNQQSFPLLAVMKSFTYTNSKSTEVTSLGAREITLEKEKTLPPKE
                     NPKPEPEAPKPDAPQAPSAPESPTEEPKKEDAPQTPQAPSTPEKQPEVPESPNPETPD
                     APSTPKDEPQAPSIPEEKPQVPEEPKQEAPSAPSTPEKQPEAPESPTEEPKKEDAPAP
                     STPEKQPEVPESPNPETPDAPSTPKDEPQVPSIPEEQPKETPAPEEPKKEDTPQTPQA
                     PSTPKEEAPKEEVPTPPAPSVPEEQPKETPTPEVPKQEDVQPEAPKSDKVESDKQMPE
                     TKKPDMKQPKADDMPKEQKPKADEPKAEQPQMDKPQMEAPKKDSEAPKSDKVETDKQL
                     PETKQPDMKQPKADDMPKEQKPKADEPKAEQPQMDKPQMEAPKKDSEAPKSDKVETDK
                     PMPETKQPDMKQPKADKPEAEKAQMPRTEGMKPESKASMMPKAEAPKATLPNTGEASS
                     AIGWLGGALATLATGLYLFKNKKEE"
BASE COUNT         5925 a         3854 c         4421 g         5452 t
ORIGIN      
        1 aagaagaaaa tgtaagaatt taaaaaaatt aataaatttt tattgcaaaa tccgaatgtt
       61 agtagtaaat gaagaaaatc cgtttgtttc ttatgatata atcaagaaag aatgacctat
      121 atggaggata agatggtttc aacagtaacg acactcggtg gatttacctt tgataattgt
      181 ttgatgaatg cagcaggggt ttggtgcatg accaaggaag aattggatgc ggttaagaac
      241 tcgaaggcgg ggacttttgt aacaaaaaca gcaaccttgg actatcgcgc gggtaatcca
      301 gaaccacgtt atcagaatgt tccgcttggt tctatcaatt cgatgggctt gccaaatcag
      361 ggcttggctt actatttgga ttatctacta gaattgcagg aaacggagcc agaacgcaca
      421 tttgttttat ctgtagttgg aatgtcgcct gatgagactc atgaaatttt gagaactgta
      481 gaagccagtg attttaaggg gctgacagag ttaaatcttt cttgtcccaa tgtccctgga
      541 aaaccgcaga ttgcctatga ttttgaaacg acagaaacca tcttgcagga ggtcttcact
      601 tactttacca aaccactcgg tattaagtta ccaccttatt tcgatattgt gcattttgac
      661 caagcagcag cgattttcaa ccagtttcct ttgaagtttg tcaactgtgt caattcgatt
      721 ggaaatggtc tttatatcaa ggatgagtca gttgtcatca agcctaaaaa tggttttggt
      781 ggtatcggtg gagagtatat caagccgact gctcttgcca atgtccacgc tttttaccaa
      841 cgcttgaaac cggagattca aattgttggg actggtggca tcttgacagg tcgggatgcc
      901 ttcgaacaca ttctctgtgg tgccagcatg gttcaagttg gaacaaccct gcaaaaggaa
      961 ggtgtggcag cctttggacg cattacggct gaattgcagg ccatcatggc tgaaaaaggc
     1021 tacgagacca ttgaagattt tcgtggcaag ctacagcaag atgactgaga tcatctgttg
     1081 aagacataaa aagaagctaa cattgtactg ttctcccaga tagtctttta gggagtcgcg
     1141 aggggataat gttagctctt tttaaatggt ctgtgaagat cggtctgata atcgtttgag
     1201 gaatggaata catagtcaga aacatatatt tgtgagatgg aattctgatt ttatttagat
     1261 tttttagcaa aaatatcgta tactaatatt agaacctcta tttataagtg atgtgctttt
     1321 atggaagaaa tagttttttg agggactgct gactgtattt tgaacaaagg ttcttaagta
     1381 gggagtatga gggtaaaggt atggagagaa gggcgaacta tattcgcaga aaacgatatg
     1441 cgtcctatcg gacaaagatg catttgtttt ttatttgtgg cttagcgact attatcttgg
     1501 cgattattat tgtaagcgag cacctagttc ctaggctgaa tcaggcctca gaatcaatgc
     1561 actcagataa ttctgttcgc atcatggctc atggggattt gctctatcac ttgcctattc
     1621 ttcgaggagc agaacagcca gatggaagtt acgatttttc tgagaatttt acctacgtaa
     1681 agccctggat agaacaggct gatttcgcta tcgcagactt tgaagggacc atttctccag
     1741 atatcgaatt agcaggttac ccacttttta atgcgcctgc cattgtcgcc aacaatatcc
     1801 atgatgcagg ctacgatttg gtggacttag cccacaatca tatcttggac tcacatctat
     1861 cagggctgat ttccactgtc aatacattta gagatgcagg tgtggataca gtcggtgtct
     1921 acgccgaagg caatcgggca actgcaccac tctacattcg ggaagtcaat ggtatccgta
     1981 tagctgtcct agcctatgct tacggtttca atgggatgga ggctttgctg agtcaggagg
     2041 aatatgatgc ctatttatcg gattttgata aagaaaagat gcgggcagag attgagcgag
     2101 ctgagaaaga ggcagatatt accatcgtca tgcctcagac gggtgtagaa taccagttgg
     2161 agcctacgga ggaacagacc agtttgtacc atcaaatgat tgattgggga gcagacatcg
     2221 ttcttggtgg gcatccgcat gtagtcgagc cgacggaaat tcttgagaaa gatggtcaga
     2281 gaaaattaat catctattct atggggaatt ttctatccaa tcagcgtatc gaaagtatgg
     2341 aagatacccc aaatgcccag tggacagaaa gaggtgtctt gatggacctt actattcaga
     2401 aagagaatgg gcaaacaagt attcaaacgg ctcaagccca tccaacctgg gtcaatcgcc
     2461 tttcaaaagg gacttattca acagatggat acgaacaatt ttcctaccag acttacattt
     2521 tggaagactg ggtcgctggt ggtcgatact atggacaatt ggatctagat acacaggcgc
     2581 gtgtggatac agcctatcaa gaaatgaaag actttgtcaa tttggtatgg tgaggagaga
     2641 gaagatgata gttcgattaa ttgcaacaga catggatggg acctttttag atggtcaggg
     2701 gaattttgat agagagcgat tttcacgggt cttggatcaa ctagatcaga aaaagattcc
     2761 ttttgttatc gctagtggta atggaattgg gcgattacta caattatgcc aaggatttga
     2821 agacagatta atctttgtag cggataatgg agcccatgtg tatcaaaatg gcaagacagt
     2881 aattcgtcgg gctattcagc aagtggaagt cgaagcaata ctgcattttt tcaaaggccg
     2941 ttgggcggat gtatgtctga tgttgtcgaa cgatggaaat atttatatgc aggctggggc
     3001 aggtatgccg tttgcaggaa ctgatttgcc aatagaacta gctcagatgg ctgccttcca
     3061 aaatcgtgtg acatatttag acaaccttag tgcctatcca gtttcagagt caatacacaa
     3121 ggttggtctc tgggttcctg aagcgcgtgt agaatcgata acggaagcct ttaatcaggg
     3181 ttttcacggt cagttggtgg ctgtaaccag tggttatggt tccgttgaca ttctaccgca
     3241 gggtatccat aaggcttggg gcttggaaca ggttttgacc ggtctagata tcgaacctga
     3301 acaggtaatg gccttcggtg attcagacaa tgatattgag ttattgtcct atgttgggta
     3361 ttcctatgcc atggaaaatg cgacagacaa ggtcaaggcg gttgccaaat acatggctcc
     3421 aagccatttg gaagctggtg tgcttcaggt cattgaagaa tatatcttat aagtatatag
     3481 aaaaaagcta gtgctctcaa tccagagaat gctagctctt ttaatttcca acgctggtga
     3541 acttggtcat accatagcgg aagaggttat aattgatgga aaataggacc actaccacca
     3601 gaggcaacgc cagtccccac atagacaagt ctaggaagta gagagcaggg aagtaggcgg
     3661 taaaggcgta ggggaaaaca aaaatcagca ggatttgaat gatttttgga tagatattga
     3721 gtgggtattg agccatctga ttgagggcga aaattcccat agtcaaagga aatgattcca
     3781 caatccaaaa tgctagagca gttggaccga gctgaatggc tgcatagagt aggccgatac
     3841 aaatggcaat aaagattagt aaggctagtt ttccaagact ccattccatg cctagctcat
     3901 ttgaagcttg gactagtgca agggaaccga tgagggttgt tccgattcct tgtggctgga
     3961 ttcgttcaca gataagttga aaaagtgggt tcacaggcat gaggaggagg cgttcaaatt
     4021 cgccttgtcg gatgtagcga ctgccaaaca tccagagatt gtcaaagaat atcaggtgaa
     4081 tggagcgtcc gacggtcgct attccataga taaaaagcat ttgaccgtag ttgaaaccag
     4141 cgatttcctt gatatttcca aagagaatat tgaggaaaat caggtagaca atttgttcaa
     4201 tcaaagagga gaaaagcccg atgaagaagt ccacccgaaa ggacatctgg gccatcatgt
     4261 agaccttgat gttatagaga tagaggctga tatatttctt catgttaacc tccaaaaatg
     4321 acaatacgac gcttggcttg ggaccaaaga gcagcaatga agcaggctag gatgggtatc
     4381 catataactt gcaatcccaa agcagttaga tcgctttcct gtcccaataa aatggaaact
     4441 ggaacgttaa ccgcataatt atagggaagg agcttcaaaa agttttccac agcctgtgga
     4501 tagaagctga gtggcaaaag cgctccagaa gctagattga aaagtcccat tctcagaagc
     4561 atgactcccc aggcattgac cgtaaaaaat gctaagaagc caaaagccat atccagtaat
     4621 tgaacgataa acatccctag gatggagctg actagaaaga gcaaaatagt ttttccgtca
     4681 ggtagataaa aatcctgatg aacaagtaag atgccgataa atacgatagc aaattttatc
     4741 agttccaaga gcttatttcc aaggtctttg aaaaagagag ctaggataaa ggaatagggt
     4801 ctgagaaatt ctccagcaat gcttcctttt aaaatgctgt aggataaatc ttctccgagt
     4861 gagaaagaat ttaaactggc tagtagatta gcaaagatga tatatgtagt aaaggtttgt
     4921 agggtaaagc cattgacttc tgattgagtg aagaaaatgg ttttccagac atagagagct
     4981 accaaaatct tcactgccag tgaaacgaag gtcgctagaa agaaagcctt atactgcata
     5041 gcattcatca tggtcaggcg ggtcatgtag agatatttac gcatggatac cctcctcata
     5101 aatctgacgg accatggttt caatttccaa ttccttcatg gtgacatctt caattgaata
     5161 atgattgaaa acttgctcaa tcacctgggc gctggtccag accttgcttt ggtagtggat
     5221 ttcaaagtga aaatcatctt tacgaataaa ttcccagccc tccaattgca aatctttttc
     5281 aattggtttt tctgtggaga agagaatggt cttgactgtg gaaaagcgtt ctttgaggac
     5341 ggtcagtgaa ccatcgtaga ccttcttgcc cttatcaatg ataaaaatcc gctcacagag
     5401 gctctcgatg tccttcatat catgggaagt aatcaaaaag gtggtctggt attcttgatt
     5461 catatagcgg atgaaggagc gaatggtctc cttgacgctg gcatccaagc cgatggttgg
     5521 ttcgtccaaa aaaacaacgg caggctggtg gataaagata gccgcaaatt cacagcgaac
     5581 tcgttggccg agtgacagat tacggattgg ttgcttcata atatcagcca agtccaatag
     5641 ctcaatcaag acagccaatc gctctttgaa taccttatct ggaacatcat aaatttttgc
     5701 gtgaaggata aaagattctt ggacaggtaa gttccaatcc aagtgagatt tttgtccaaa
     5761 gagaacgccg atttgcttat tgacagcttt gcggttttct tgtggattga gtccgttgat
     5821 acgaacctgt ccttggtctg ggaagaggac tccagtaagc attttgatgg tggtggattt
     5881 accagaaccg ttggagccga tatagccgat gatttttcct tttcctacct gtaaggaaat
     5941 gtcttgaaca gcagggattg actttttctt tggcttgaaa aaggccttga tagagccttt
     6001 tagaccgact tccttatcaa tgattgaata ggtttttgaa agatgttctg cctgaatcat
     6061 atcataatct cctatttgaa aacgtttgcg taagataagg atattataac atattcccca
     6121 gaaagtggcg gtaaaaagtc gcctttaagg taacattttt attagaaaat cggtcatata
     6181 ttgaggagat tcttgcttac catttttaat ccatacctgc atgataccga agaaggcatg
     6241 tgaaaaatag atgctgctgt attctttttc cagttgtgat aaattctctt ttcctaagcg
     6301 ctccgctaaa tcattggcaa caagaagtcg gactttattg ataagaaaat tttggatttc
     6361 ttgagttccg ttatgggtca gtagggcaga aagcaatttt tcacgataga gcaactcgaa
     6421 aatttctaaa aatgatgatt ggcgattgcc atcataatgg tcaaatatag cctctaattt
     6481 gtgaaaaagt ccctgttgat aggagtcgat taattcatat ttatccttat aatgggtata
     6541 aaaactggaa cgactgatac cagcagtctg ggctaggtgt gttgttgtaa tgtcattaaa
     6601 tccttgtgat tccaggcagg ttaccatggc ttctaaaatt gcctgtcgag ttcgttcttt
     6661 ccgactatct gttctcataa tttctccttt tgaacatttt tagacacagt gtctaaaaac
     6721 agttcttctg acttgatttt gaaattgata cttgtataat gtattttatc agtttttgga
     6781 cagtatgtct aaaggagttg cttatgttac aaaaagtaaa gaatattttc aaaaaaccaa
     6841 tgaccttgat taccattcta ggtattgcct gtgtcccagc tttgtataat attagttttc
     6901 taacttctat gtgggatcca tacggccgac tagaccagct gcctgtagca gttgtcaatc
     6961 aagaccagtc ggcgagcttt caagataaaa ccctcacaat tggtgatgat atggtggaca
     7021 atatgaaaga gagcaaaagt ctggatttcc actttgtatc agaaaagaat gcggagaaag
     7081 gcttggaaga aggggactac tatatggtca tcactctacc agaagattta tcagaaaaag
     7141 caaccagtct cttgacgaat cagcctgaac cgataacgat ttcttatcaa acctccaaag
     7201 gtcatagctt tgttgcttct aagatgggcg aatcagctat ggagaaatta aagacatctg
     7261 tttcagagac aattaccaaa acctacacga ctgccgtttt tgatagtatg agagaaattc
     7321 aaaccggcat gatcgaggcg gcagatggta gccaaaaatt aacaaatgga gctagtcaat
     7381 tggagtcagg cagccaaaca ctttcaaatg gactgactac tttaacgact tcgggtcaag
     7441 tactcgtgac aggagcaaac caattggcca ccggagttgt gtcttataca gatggagtca
     7501 atcaggctgc aacaggcagt caaacactgt ccagtggctt aacgatttac acaaatgggg
     7561 tagcgagtct tgtttcaggt gcggagcaat taaatgccaa ttcctctcag ctcatcactg
     7621 gggtgggaca attgcaatca ggagcaagcc aggttgaaca acttgtaacg ggagccaatc
     7681 aattgcaggc aggcttggaa caactcgcta attcaactag tttgtctgct gaacagtcta
     7741 gtcagataca agcactcttg acagggcttc cacagttgca ggtggctatc aatcaattaa
     7801 atgacagcct atcaagtatc ggaggacttg cagttgataa ctctactcta tctagcctct
     7861 tgacggagat gggggctcag gctcaaggct tgttgacagc agcccaagct gataaaacag
     7921 ccagcatcga agccttgcaa accacggcta cctatcaaaa cttgacagct gatcagcaag
     7981 ctgaattggt tggtgccctc caaaattcac cttcggcaac agcgacagta gcccaaacga
     8041 ttttggatca attgtctcag ctcagtcaaa ccctttctag cctacaaagt ctttctggca
     8101 tggcaacaca aatgaaccag ctacaatcag ctgtcggtca gattaacacg gctgccaatc
     8161 aagcattgcc aggagctaca acagccattg aaagcctatc aagtggcttg agtcaggtca
     8221 acacagcttt gaaccagcaa gttctaccag gaactcaaac cttgacgagt ggtgtgagtc
     8281 aacttcaaac gcaactgtca agcggagcga gtcaactcat gtttggagta acagcctata
     8341 ccgcaggtgt tgctcaatta gcagcaggtg gagcccagtt ggttgcgaat aatagtagta
     8401 tccaatctgg gggtggccag ctaacatcag gattggcaac cttggcatcc aactccagtc
     8461 aacttgtcag cggttcagaa caactggctt ctggtagtca gcaattaatt gctggtgcgg
     8521 atcaattagc tagtggtggc caagccttga caagcggtat ttctagtctt cgtacaggta
     8581 gtgagaccct aacgaattca ctcagctcag ccagtcaaca actatcagtc gtctctgtag
     8641 aagacaaaaa tgcacaagca gtttctcaac cagtcacctt ggaacacagt gaccaagatg
     8701 atgtgaagac caacggtgtc ggaatggctc cttacatggt atcagtcgca ctgatggttg
     8761 cagccttatc agcgaatgtt atttttgtca aacacattga taatcgctcc tacaagaatc
     8821 gttgggactg ggccaaagga aaactcttgc tcaatggtat cattgccagc ctagcagcag
     8881 tgattctgta cggtgttctt cgcttgattg gaatcgaacc agctcatcca atggcaacct
     8941 tgggaataat cctcttagcc tcttggacct tcatggccct cgttacagcc ttggtgggct
     9001 ggaacaatcg tttcggatcc ttcgcaagtt taattatctt gttactacag cttggatcaa
     9061 gcgcaggtac ctatccaatt gaactcagcc cacgcttctt ccaagtcatt cagccctatc
     9121 ttccaatgac ctattctgtt tcaagtcttc gtcaaaccat ttctatggtt ggaaatagta
     9181 gtcatcaagt ttggatgcta agtctcttct tagtaggctt tatgggacta ggtctcttga
     9241 tttataatca aaaagatgaa taagaataaa accaactgct ccatgtgaac agttggtttt
     9301 tggttgatgt ggttatttgg gggtccctaa attgattgtc gcattggtgg cttcaatggt
     9361 cagtttgttc ttgctacctt tgttttctag ggttagggtc tgtagatcgt tttcagtctt
     9421 gacagggaat ttggtgtact tatcgctagt gaaaataccg acttccccta gtcgctcttt
     9481 taagtaaggg tcttggttaa ccatctctcc caaatcattc ccatcttcat agtagtaacc
     9541 ttgggcttct actaattttt tcttgtcgat ctggttggtg atgagcaagt tgctatctgc
     9601 caggctcttg tccgtcacat ccaaaaatgt cgtagaggag taggtgtcgg atggagtgaa
     9661 acgattatcg ccaagaagag tcgtttgtct gctatccagc tggctatagc cctcgatggt
     9721 cacattttcc aaactggttt catacaggcg gacgctgctt ccctgtaggt tgatgtgcat
     9781 atcttttaga gtggattgtg tcgcttggaa gtagcctgct tcaatctgac cgctgtcaat
     9841 agtcaccttg tcaagagtga caccaccaga cagatttgcc tctttgatat ggatattctc
     9901 aatcgtgaaa ccgccaaaat catagaagtg gagattgtgt ccctctaatt tatccagtgt
     9961 cttcccttta ggcacttcaa tggtgacgga gaagacattg atacgacgct gggccaagct
    10021 ttctcccaga tactgcatga ttccctcaat ggcaaattct agttcctgtg cagagagggt
    10081 taagacgccg tctttctcac tgagttttag agggttttgg atattgttgt cggagttggc
    10141 ataggtcaca tgatagttgt tatcactaga ctctttgatg tagacgctgt gggggataaa
    10201 atcaatttta atggtattca aatctgtgaa ggtcttatgg acttccgtcg gagcagcaac
    10261 ctcttctaag cgggttatgc ctccggtgaa aaatccaatg ccagctagaa ttaagcctgc
    10321 aatcaggctg acaaagccga taataatggc gatggtaagt ttctttttca tgctcgttta
    10381 cctcgtttca aaatccattt gaagcaagcc ttgaccagtc gccaagacca gtaggcaaaa
    10441 aatccagtca agatgtagat gagaatagct ccgccaatca aagacaaacc tgacccaaac
    10501 cccatgagga agatattgaa accttctgac ataaggctaa aggcttccca aattagatag
    10561 ccaccgctgg tgagaagtcc gacacccaaa acaaaggcac cgacaatgag ggccaagatg
    10621 agcgccacca atcccaatag actagctagg agaatgagca aaagtggaat ggcgataggc
    10681 agggaaatga cagctaggat agccaaccag agcgttcgtt ttgtttggct aaaattacgg
    10741 aagtagcgac cgaggaaaaa agcgccgagg ataagagtcg caaacagagg aatgactccg
    10801 ataaactctc ccatgataaa gagtaggaag aaagagaaga gacctgtcat acttaaggct
    10861 cccagaccaa cccagagttt ccagttgagt tgaataggtt cttgttgatc cttctcctcc
    10921 tggatttgct tgttgagcat gttgttaatg agttcgctgg cagcttcttt tggggaacca
    10981 agttcttcta taatttctgt ttcacgctct ggtccaacct cgtcaaaata ctcattaaag
    11041 aagctgatgg cttcaaagta ttctttgtgg ggcagttttt tgagatattt ttctaactgc
    11101 tccatatatt cagttcttgt catggcggac gctcccttct atgatgccgt ttatcgtgtt
    11161 tgtataggta tcccagtcat ctttgagtcg gaccaattct tcgtgaccta actgagttag
    11221 gctataatac tttcgcatac gaccctgata ttcttgggaa taagtagtca aaaagtcatt
    11281 ttgctccaat cgttttagga tgggatagag ggcggactcc ttgatgttgg caattaattt
    11341 aatggtctga cagatttcat agccataaga atcatcggat tccaaaatag ccatgatgag
    11401 aaattctgtc agcactgcgg ggactggaaa atgcatagga cgctcctttc ctttgttgtt
    11461 agttaggttc ggttttgata tatagtgttc ggttgcatat atcaaatttt tctatatatt
    11521 aaatatacac ctacaaaaat aaattgtcaa gtgattatgt gcaaaataaa taaaataact
    11581 taagaccaaa tagcttagga caaattttta aggtgtaaac gttttatagc cttgaaaaat
    11641 aatttcagag tagtaaactt aataacagta ctaacattaa gggattttct ggactaaaag
    11701 agaatgacct cagatggatt agtagtattt aaggtggtgt aaagatgaaa ctatatgttc
    11761 agtttatgat tattctggta ttttcatttt taggggaagc tatttcgact atttttcatc
    11821 ttcctattcc gggaagtatt attggcttaa tcctcctatt tttagcccta gagttcaaat
    11881 tgattcgtct tcgccatatt catacggttg ggaatttttt gctggctaac atgaccattc
    11941 tttttttgcc tgcagcagtg ggcattatgg agagatttga tgcaattaaa gattatttac
    12001 ttcctattat cgtcgtcatt ttaggcgcca tttttttaaa tatcttagta attggttttg
    12061 ttgttcaatt tgtgaagcaa aagtttgaag gcgactacat tgatgcggag ggaaatcatg
    12121 actgaacttt tttccaatcc tatttttggt attatgttat caattgcagc atatttaatt
    12181 ggaatgctga tttatcggag atttcctcat ccaatcacaa ccccactgtt ggtagctaca
    12241 ggattgatta ttgtcttttt aaaaatgaca ggtatttcct ataaggaata ttatgcagga
    12301 gggtcctatc taaatatgct tattgtgcct tcaacagtag ctctgggtat tcctttgtat
    12361 cgttctttcc atctaatgaa acatcacatt cgaagtattc ttttgggaat ttttgttgcc
    12421 tgtattgtca atactgtctt tactgcccta attgcaaaat ggtttgggat ggattttttt
    12481 ctagcaatct ctctttttcc aaaatcagtc accactgcta tggctgttgg tattactgat
    12541 aaaatgcaag gaatagcaac agttactctt gtagtcgttg ttgcaacagg aattctgaca
    12601 agtgttcttg gtccagtctt tttaaagcta ctaaaaattg aagacccggt agctgttggt
    12661 cttgctttgg gaggaacagg acatgccatt ggaacgggta cagcgataaa atgtggacat
    12721 acacaaggag cgatggctgg cttggctatt gggattacag gcatcatgta tgtggtgatt
    12781 agtccaatag ttgcgcagat tatcttacaa taaaaaacta cgattggaat tccaaccgta
    12841 gttttttagt ctacataaag tttggattga ttattcaacc aactataaag aagtccgata
    12901 aagagaccgc ctcccacgta gtttcctaat ccagaaaaga ggaaattgct tagtacactt
    12961 aatagagtca ttccctcaac tggtccacca tttgcaaaaa aggccaggga gaagagtgaa
    13021 aagttcgcaa tgacgtgctc atatcccaag aaagcaaaga taaagatgat gaaaataacg
    13081 gaaatgagac gtccagcgtc atctttgatg cgtaagaagg taaatacgcc gatattcacg
    13141 acaatattag caaaaattcc ttcaataaat tgagtcaaag gtgtctttga aagcttggcc
    13201 accgttgctt caaataggta gctgtgagca tcaacgtgct gataaggcag agtcagtgag
    13261 accaagtaac aaacaaggac agcaccgaca aagttgaaaa ggatacaggt tgccaacatc
    13321 ttgagggctg ttttagtagg aatcgttttt cgatggctgg ctactgtcat atacatcatg
    13381 ttggatgttc caagttcggc attcatatat aaaatcatga gtagccccca accaaacatc
    13441 aacccatagc taaatttccc gagtccttct gcgacatggt tgagtttgtc tgctgtataa
    13501 agagaaatga caatccccaa tcctaaataa agactggcta gtattgatcg tacagcgtag
    13561 gcagaaaaac tgtgttcaaa caaatctgct tttttcttga tactcttttc aagtgtatac
    13621 attaatgtct cttggttagc tcccatccta atctccttac tgaatctact actttttcga
    13681 gtaaacgttt tctttttatc gaatggcttt actttgttta ttatacttga taaaatggtt
    13741 gttgtccaaa aaaaattcaa cttttgaaag gaaatataag aaaaatatgg atgttacatg
    13801 gacagtgaaa tatatcactg aatttttagc gactgcactc cttattatca ttggtaatgg
    13861 tacagttgca aacgttgatt taaaaggcac aaaaggaaac aactctggtt ggattctcat
    13921 tgcgattggc tatggtttgg cagttatgat gccagcattg atgtttggta acgtttctgg
    13981 taaccatatc aacccagcct tcacacttgg tttggctgtt tcaggtctct tcccttgggc
    14041 tcacgttgct caatacattg ttgcccaatt gttaggtgca atgtttggtc agttagtggt
    14101 tgtagcagtg tataaacctt acttcttgaa aactgaaaat tctaaccata ttcttggctc
    14161 attctcaact atcagtgcgc ttgataacgg aacgaaagaa agccgtaaag ctgcgacaac
    14221 taatggtttc ttgaatgagt tcgttggttc atttgtattg ttcttcggtg cccttgcttt
    14281 gaccaaacat ttctttggtt cagagttggt tggtaaattg attgaacagg gctatgataa
    14341 aacagtcgct gagacaatga ctgctccata tacttcaggt tcaattgcag ttgcccactt
    14401 aggcattggt ttcctagtta tggctcttgt tgccgctctt ggtggtccaa caggtcctgg
    14461 tcttaaccca gcgcgtgacc ttggtccacg tatcgtccat gcccttcttc caaaatctgt
    14521 tcttggtgaa aacaaggctg attcaaaatg gtggtatgct tgggttggtg tatgtgctcc
    14581 aattatcgca tcaattgccg ctgttgcact tttcaaattc ttgtatctat aatatagaga
    14641 acccgtttcg tcgggttttt ctttttctct tggtatatcc tatataaagt tgtctgctct
    14701 gctattttcc taaaagttat gctacaataa ggatagaaaa atcggaaaag aggctagaat
    14761 atgtcagatg ttattgcttt tggctatgga agcagtcgag cgaagatgca gctcaagcgg
    14821 ctcaatcgtc atggaatcat cgcaggtgcg acaggtacag ggaagaccgt gaccttaaag
    14881 gtcttggcag agcagttgag tgatgcggga attcccgtct ttctatcgga catcaagggt
    14941 gatttgaata gtttggttgc ggccaatacc aaggagattg atcctagtcg tctagagaag
    15001 actcattatc ttgactatag cccagtcggt tatccagttg agttgtggga tgtcttgggg
    15061 gaaaatggga ctcctatccg catgactatt tcagagctgg gacctgtcct actgacacgt
    15121 ttgttgggct tgaatgatac tcaggagtcc atcctgaata ttgttttcag cgtggcagac
    15181 gagcgtggtc tgctcttgat tgatttgatg gacttgcggg ctatgttaaa ctttgtggct
    15241 gaaaatgcgg cagagctgag ccagtattat ggaaatattc cagctcgttc ggtcggtgct
    15301 atcttgcgta gtctggttgt tttggagcaa caaggtggta agattttctt cggtgagcca
    15361 agccttgata ttgctgattt gatgcgtacg gcagaagatg gtcgtggtgt tattaacgtc
    15421 cttcaagcta ctcagctatt taatcagcca acattatatt caacggttct tctcagcctc
    15481 ttatcagaac tctatgaagt cttgcctgag gtgggcgatt tggacaagcc taagatggtc
    15541 ttcttctttg acgaggctca tgttctcttc aaagatgctc caaaagttct tcttgaaaag
    15601 attgaattga ttgtccgttt gattcgttca aaaggtgtag gtgtcttctt tgtaacgcag
    15661 aatccgacgg atattcctga tagcgtggca gcccagttgg gtaaccgcat ccaacatggt
    15721 cttcgcgcct ttacaccaaa agagctcaag acagttgcta ctgtcgctga aaccttccgt
    15781 caagaaggtg atgaggattt ggccaaggtc attcaggagt tgcaagtggg ggaagctgtt
    15841 gtgtctaccc ttcaagcaga tggtacacca agctttgcgg atcgggtttt gatttatcca
    15901 ccaaagagta tgctgggaac ggtggagcca agcgccctct tgtcagttat caataattct
    15961 cctttgatgg aaaaatatgc tgacgcggtc aatcgtgaat cagcccatga gcagatttta
    16021 gccatgacag aggctaaaga agccgaactc atcaaaaaag cagagcaggc cgaggcggaa
    16081 aaacaggcag aaaaagaagc taaggcagcc gcaaaaatgg aagagaaagc ccagaaagaa
    16141 gcagctaaaa ctgcacaaaa aactagtcag ccagccagtc gcaaaacaga ctctatgatg
    16201 gatcgtttta ccaagaacct gatgagtcag gtcggacggg aagttgggcg cgtggtaact
    16261 cgtggcatta tgggcatgtt aaaagggaaa taaacgacta gactgggaag gaaacttctc
    16321 agttttttat ctagacaaag ttcttgccag atgccacaaa attcgttaaa ataaacacaa
    16381 atcactagtt agtaaggagt tgccatgact gatcaattgc aggcattatt gaaagactat
    16441 cagccccagc cattgggtga aaaacgttct tatgcagttt tcttgccctt agtctggtca
    16501 gacaatcagt ggcaggtctt atatgagatt cggagcgagt ccatttctca accgggagaa
    16561 gtttcctttc caggtggcgg agtagaggta ggagaaacgc cacagcaagc ggccgttcgg
    16621 gaggttatgg aggaactaaa tattcaacct gaacaaattg atatactcgg agaaattgat
    16681 tatttggttt tggagtgctc aaccgttcat tgcttcgttg gacggttaaa tcttgattgg
    16741 acgactatcc ttcctaatga agaggttgct cggatattta cagtgcctct atcaactcta
    16801 ttaacaactc agccagtcta ttatcaactg gattcacaga ttgtcccaga ttgtgacttt
    16861 ccctttgaac gtttgagagg tggggttgac tatcctttca gtcatcacaa acgctcggtt
    16921 cccttttatg aaaatctatc agaaaatatc tggggaatga ccgcccaatt tacccatcgg
    16981 tttacggaaa tagtgaaaag taatctatcg tgaaatgata ggttattttt gggaggaaaa
    17041 accagccttt gcgatatgta tcaacgaaaa aaatggagac aaagctcaat gtgaaccaat
    17101 gtatataaaa acacaaaaaa aacagatgca tggcagataa aagatagcta tggctggtat
    17161 acttggtacg gtccatattt tgaagtacca tcatctatcc aggaatgcga caacattcat
    17221 gtaaacgcat tcagtgcttg ttgtcatttc cagtaggtga gtgggaaaat gtataaatct
    17281 atattttaag aaggagccaa ttatgagcaa gcagaaagtt gtgtccagtt tattattaag
    17341 cacagtcgta ttagggggat tagcctttta ttcaacgaca acggttaaag cagaatcgct
    17401 agaaccagat gttacatcag ttaatgcaag cgatgctact aataaaccgg tagtaccaaa
    17461 tgaagaggga ggcgactttt tagctgaaga ggagcttgaa gatgatgaca ctttggaaga
    17521 agaattaaat gagaaagccg aagaggttac tgagccatct tcacctgaag cacttctcca
    17581 acctcgtgcg atgatgagtg attctgaaac tagtggtatg gaagaaattc cgatgaatga
    17641 tgaaccttca gataatacgg aagaaaaggt agagaagcaa cagtcgccat taattcaaac
    17701 ctcaaatgca gattataaaa gtggtaaaga tcaagagaaa cttaggacat cagtatctat
    17761 aaatctgttg aaggcggaag aaggtcagat tcagtggaaa gtaacttttg atacaagcga
    17821 atggtctttt aatgttaaac atgggggtgt ttattttatt cttccaaatg gtttggactt
    17881 aacaaagatt gttgataata atcaacatga tataacagcc tccttcccta cggatattaa
    17941 tgattacaga aattctggtc aagaaaaata tcgtttcttt agtagtaaac aaggtcttga
    18001 caacgaaaat ggttttaatt ctcaatggaa ctggtctgct ggtcaagcta atcctagtga
    18061 aacagtaaac agttggaaat caggcaatcg tttgtctaag atttatttca taaatcaaat
    18121 aacagatact accgaattga cctatacttt aactgctaag gtaactgaac caaatcagca
    18181 atcattccct cttctggcag tgatgaagag ctttacgtat acaaactcta agagtacaga
    18241 ggttacttcg ctaggtgcac gtgagattac ccttgagaaa gaaaaaactc taccacctaa
    18301 ggagaatcca aaaccagagc cagaagctcc aaaaccagac gctccacaag cgccatcagc
    18361 accggaatct ccaacggaag aacctaagaa ggaagacgct ccgcaaacac cacaagcgcc
    18421 atcgactccg gaaaaacaac cagaagtacc ggaatcacca aacccagaga caccagacgc
    18481 gccatcgact ccaaaagatg aaccgcaggc tccatcaatt ccagaagaaa aaccacaagt
    18541 accggaagag ccaaaacaag aggctccatc agctccgtca actccggaaa aacaaccaga
    18601 agcaccggaa tctccaacgg aagaacctaa gaaggaagac gctccagcgc catcgactcc
    18661 ggaaaaacaa ccagaagtac cggaatcacc aaacccagag acaccagacg cgccatcgac
    18721 tccaaaagat gagccgcaag ttccatcaat cccagaggag caaccaaaag agactccagc
    18781 gccagaagaa cctaagaagg aagatactcc gcaaacacca caagcgccat caactcctaa
    18841 agaggaagca ccgaaggaag aagttccaac accaccggct ccatcagtac cagaagagca
    18901 accaaaagaa actccaacac cagaagttcc aaaacaagaa gatgttcaac cggaagcgcc
    18961 aaaatctgat aaagtagaat ctgacaaaca aatgccagaa actaagaaac cagatatgaa
    19021 gcaaccgaag gcagacgaca tgcctaagga acaaaagccg aaagccgacg agccaaaggc
    19081 agagcaacca caaatggaca aaccacaaat ggaagctcct aagaaagatt cggaagcgcc
    19141 aaaatctgat aaagtagaaa ctgacaagca attgccagaa actaagcaac cagatatgaa
    19201 gcaaccgaag gcagacgaca tgcctaagga acaaaaaccg aaagccgacg agccaaaggc
    19261 agagcaacca caaatggaca aaccacaaat ggaagctcct aagaaagatt cggaagcacc
    19321 aaaatctgat aaagtagaaa ctgacaaacc aatgccagaa actaaacaac cagatatgaa
    19381 gcaaccgaag gcagataaac cagaagcaga gaaagctcaa atgccacgga cagagggtat
    19441 gaagcctgaa tctaaggctt caatgatgcc aaaagcagag gcaccaaaag caactttgcc
    19501 aaatacaggc gaagcaagta gcgcaatagg ctggctaggt ggtgctttag ccactcttgc
    19561 cacaggcttg tatctattca aaaacaaaaa agaagaatag tcattattga ataagatata
    19621 agaacttccc tcttctaagt tcataaaaga ct
//
LOCUS       DBNFCK010000026        18649 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.026, whole genome shotgun sequence.
ACCESSION   DBNFCK010000026 DBNFCK010000000
VERSION     DBNFCK010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 18649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18649
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.026"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(49..3318)
                     /locus_tag="AC1PIW_000731"
     CDS             complement(49..3318)
                     /locus_tag="AC1PIW_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HIP6605687.1"
                     /translation="MIPIQQHGKRWLILTLTLSTSVLALASIGTSQPVVFADENSHLQ
                     SPKNTNKIEVLNWEAFSKKLKDYSSDQRQFHVLKLGFENRLGTLSTREELEEFGKNNN
                     FLVINGKVTQNIHDFPHILVMNKGDVIAHNEEDYHNQMRELRFSGNGDLHNSMEPKRI
                     HALFKIELDSNKRQLLNAAGLGTAENSLKNINGMTIYSHGLTVDNKYYEDYSKYTHNS
                     VKNINVTKERFIANDDLIHKLIESSEAMKQSSERDKVKAFVQYVANHTTYDWEAANKA
                     VQNYADINYYLGSDLFAVTERQKAMCVGFSTTAARAFNMLGLPAYVVVGKNAEGVPHA
                     TARVYYDKKWHTIDGTGFITGNKHQRSAKYSEKHFSTIGEDSYDVVEAGQEPKAERNY
                     MIIDSNYESWAMKQKTADLLLFNKEKSLVGLDYIAYVEPTYITENKKNHLLDIYKALK
                     RKVEETKATDKDDKDDKQEGYDRVLQFVNSDIDKLSALSKITEEEFKALENSMDLARV
                     FLGQMNAKAGKEFSEGETYQSYLKNRQKNNTNSDDDRNRNELQENQANSDEVSQNSKD
                     ASAPSVNSAQQSEELEGTPSTQETISAAPSQQTPAAPKALQAKTELEDKTETSSLGNT
                     EMVSPSSETAQNTVDSKEESDAKLPYVEPSSKESSEAQVNTVSSPQVSSASPTSSETI
                     STDTVTASQEKAESRVSAPQVISASQTSPETNSAEVVTTSQEVAKAPVSAPQVSSEIQ
                     TLSETAPTEVATEAPELSALESNPAPQTSLETTPTEEVTETPEPSVVESNSASPTSPE
                     TNSAEAVTTSQEMAEPSVSVPQVSSEIPTSSETAPAEVATEVSEESSSASQPSPETTP
                     TETVTTSQEVAEAPVSAPQVISEIPTSSETAPAEVATEVTEPSVVASSSASPTSPEAN
                     STEVVVAKQEVADPLVSAPQTSSASLTLEVPKNEHLDEKADATTPNGVEANTHEAVSV
                     PTSDIRVQDAGSDTVPQPYSLSATLFEEAISTVEPVGVATSSQERSAVAGKVKVPTSL
                     ERSNNSVEEEKVVDSNATIENREPEKKEILTSENVLNSLVTIWVALSTSFFMRYFSRG
                     K"
     gene            complement(3927..4811)
                     /gene="scrK"
                     /locus_tag="AC1PIW_000732"
     CDS             complement(3927..4811)
                     /gene="scrK"
                     /locus_tag="AC1PIW_000732"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HIP6605688.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFQVVEKTQFPTTTPYETIERT
                     VEFFKRYEDRLAGIAIGSFGPIDIDQNSQTYGYITSTPKPHWSNIDLLGLIAKEFNIP
                     FYFTTDVNSSAFGETLVRKGVKSLVYYTIGTGIGAGAIQNGEFIGGLGHTEAGHTYVA
                     LHPYDVKHEFKGMCPFHNGCLEGLVAGPSLEGRTGIRGELIELNSDVWDVQAYYIAQA
                     AVQATLLYRPQVIVFGGGVMAQEHMLNRVREKFVGLMNDYLPTPDVKEYIVTPAVAEN
                     GSATLGNFALAKQVSRNS"
     gene            complement(4939..6816)
                     /locus_tag="AC1PIW_000733"
     CDS             complement(4939..6816)
                     /locus_tag="AC1PIW_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HIP6605689.1"
                     /translation="MNNTEIAKKVIDALGGRENVRSVAHCATRLRVMVVDEGKIDKDT
                     VEDIEKVQGAFFNSGQYQIIFGTGTVNKIYDEVVALGLPTATTGEQKAEAAKQGNAFQ
                     RAIRTFGDVFVPIIPAIVATGLFMGLRGLLGALGYTLPEDLNVYSQILTDTAFIVLPA
                     LVVWSTFRVFGGNQTIGIVLGMMLIAGQLPNAWVVASGGDVKPTIFFGFIPVVGLQGS
                     VLPAFIIGLVGAKFEKAVRKVVPEVLDLLVTPFVTLFVMSILGLFVIGPVFHVVENYI
                     LAGTQAILNLPMGLGGLLIGGVHQVIVVSGVHHIFNFLEAQLVANTGANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKALAFPAALSAFLGITEPAIFGVNLRFRKPFLLSLIAG
                     AIGGAVASLLGLAGKGMGVTIIPGATLYVGNGQLFQYILMVAVSFALGFALTYLFGFE
                     DEVEAAPKAAAAPVATPATPAVELNEETLVSPLSGDVVALENVNDPVFSSGAMGKGLA
                     VKPSEGVVYAPADAEVTIAFETGHAYGLKTASGAELLIHIGIDTVSMNGNGFEKLVAA
                     GDKVKAGTPLAKFDAAKIAEAGLDDTTMIIVTNTADFAEVSPLAEGTIAHGVEFLKVA
                     K"
     gene            7022..8476
                     /locus_tag="AC1PIW_000734"
     CDS             7022..8476
                     /locus_tag="AC1PIW_000734"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HIP6605690.1"
                     /translation="MAFTTGERYRAYADWTPEYIEKIKKNTESSPWRTNFHIETPHGL
                     LNDPNGFSYFNGKWTLFYQYFPFGAAHGLKSWVHTESTDLVHFEETGTVMYPDTPLDS
                     HGAYSGSAMEFGDKLFLFYTGNVRDENWVRHPYQIGALMDKDGKIEKIDKVLIEQPED
                     TTDHFRDPQIFNYKGQYYAIVGGQNLDKKGIVKLYKAENNDYLNWSYVGDLDFANDMT
                     AYMMECPNIAFVGEQPILLYCPQGLDKEVLDYGNIYPNMYKIGQSFVPETASIVEPSE
                     LINLDYGFECYATQAFNAPDGRTLSVSWLALPDVEYPTDAYDYQGCLSLVKELTIKDG
                     KLYQYPVDAITSLRKEAQPFSAQKETNNVYELELDFSASTKHEIVLFANEAEKGLVLS
                     VDTEQGQITLDRGNCGQPFALDFGTSRSCEISPGALTANIFIDKSVFEIFINKGEKVF
                     SGRVFPDADQSGIVITTGNPTGTYYHLDYGRKTN"
     gene            8457..9422
                     /locus_tag="AC1PIW_000735"
     CDS             8457..9422
                     /locus_tag="AC1PIW_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIP6605691.1"
                     /translation="MVAKLTDVAQLAGVSPTTVSRVINKKGYLSEKTIRNVEEAMREL
                     GYKPNNLARSLQGKSAKLVGLIFPTINNIFYSELISHLEKELFDRGYKTIICNSQHES
                     DKEREYLEMLAANQVDGIISGSHNLGIQDYDRVVAPIIAFDRNLSPSIPIVSSDNFAG
                     GVLAAQTLQKAGCHNPLMITGNDDSNSPTGLRQVGFTSILNKAEVFHISSDFSPVRKE
                     MEIRAILEKQKPNGIFVSGDATAMLVWNVIRSMKLSIPEDIKLIGYDGTSFIENYYPQ
                     LTTIKQPLTEIARLLVELLVDKIEGKKLTKTDYILPISLLAGASI"
     gene            complement(9502..9921)
                     /gene="nusB"
                     /locus_tag="AC1PIW_000736"
     CDS             complement(9502..9921)
                     /gene="nusB"
                     /locus_tag="AC1PIW_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIP6605692.1"
                     /translation="MKNNRHALRKCALQAIVSLEFGQEPVQAAQFSYLYDREEEQEGV
                     EIPLFLLNLVNGVADYRDDLDKELSSRLKAGWTLDRLTLIDKNIMRLGLFEILHFEET
                     PDRVAVNEAIELAKEFSDESSAKFVNGVLSQFIKEEA"
     gene            complement(9911..10303)
                     /locus_tag="AC1PIW_000737"
     CDS             complement(9911..10303)
                     /locus_tag="AC1PIW_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HIP6605693.1"
                     /translation="MTTEYQGEIVIAPRVLEVITGIAAAKVDGVHSLQNKRVADSWSK
                     TSLNKGVYLETDEEGRVTADIYVYLEYGVNVPAVSMDIQRAVKTAVYNYAEVQVAAVN
                     IHVSGIVPDKTPKPALKDLFGEDFLDEE"
     gene            complement(10331..10888)
                     /gene="efp"
                     /locus_tag="AC1PIW_000738"
     CDS             complement(10331..10888)
                     /gene="efp"
                     /locus_tag="AC1PIW_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIP6605694.1"
                     /translation="MIEASKLRAGMTFEAEGKLIKVLDASHHKPGKGNTIMRMKLRDV
                     RTGSTFDTTYRPDEKFEQAIIETVPAQYLYQMDDTAYFMNTETYDQYEIPVATIKEEL
                     LYILENSEVKIQFYGTEVIGVTVPTTVELVVAETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIEAGQKLVINTAEGTYVSRA"
     gene            complement(10952..12013)
                     /locus_tag="AC1PIW_000739"
     CDS             complement(10952..12013)
                     /locus_tag="AC1PIW_000739"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIP6605695.1"
                     /translation="MQKRLEKFEAKLAQSTVDGILVTGQNNIYYLTGFWGTEATVFIS
                     GKRRLFVTDSRYTLIAKASVKGFDIIESRFALEEIAKVIKEDGLEKIGFDSEVTYGFY
                     QSLTSIFEGYQLVAMSNFIEDLRMIKDEKEIATIRRACQISDQAFIDVLDFIKPGQTT
                     EMDVNHFLDHRMRQLGAEGASFEFIVASGYRSAMPHGRASEKVIQSGETLTLDFGCYY
                     QHYVSDMTRTIHIGHVTDQEREIYDVVLRANKALIEQAKEGVTYREFDAIPREIISAA
                     GYGANFTHGIGHGIGLDIHEYPYFGKSDETIKAGMVLTDEPGIYLDDKYGVRIEDDLL
                     ITENGCEVLTLAPKELIVI"
     gene            complement(12112..14937)
                     /gene="uvrA"
                     /locus_tag="AC1PIW_000740"
     CDS             complement(12112..14937)
                     /gene="uvrA"
                     /locus_tag="AC1PIW_000740"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIP6605696.1"
                     /translation="MQENIVIHGARAHNLKNIDVTIPREKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMDKPDVDSIEGLSPAISIDQKTTSRNPRSTVGTA
                     TEINDYLRLLYARVGVPYCINGHGAIAASSVEQIVDEVLELPERQRLQILAPIVRKKK
                     GQHKTIFEKIQKDGYVRVRVNGGVYDVSEVPELSKSKAHNIEVVVDRIVIKDGIRSRL
                     FDSIEAALRIADGYVIIDTMDEKEMLFSEYYACPVCGFTVPELEPRLFSFNAPFGSCS
                     DCDGLGMKLEVDTDLIVPDASKTLREGALAPWNPISSNYYPQMLEQAMNHFGVDMDKP
                     FEELTEEEKNLIFNGSDGKEFHFHYENEFGGVRDIDIPFEGLITNINRRYRETNSDYT
                     RTVMKTYMNELTCGTCHGYRLNDQALSVKVGGEQGLHIGQLSDLSVADHLQVIEHLTL
                     SENEATIATPIVKEIKDRLSFLNNVGLNYLTLSRAAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADWLIDIGPGA
                     GVFGGEIVASGTPAQVAKNKKSITGQYLSGKREIPVPLERRVGNGRFLEVTGAKENNL
                     QDVTVRFPLGKFVAVTGVSGSGKSTLVNSILKKAIAQKLNRNSDKPGKFKSISGIEHL
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVFVPCEVCHGHRYNSETLEVHYKEKNIAQVLDMTVNDAVE
                     FFKHIPKIERKLRTIQDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSLYIL
                     DEPTTGLHTEDIAQLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDMGPEGGVGGGT
                     VVATGTPEEVAENPASFTGQYLKMKLK"
     gene            15441..16385
                     /locus_tag="AC1PIW_000741"
     CDS             15441..16385
                     /locus_tag="AC1PIW_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HIP6605697.1"
                     /translation="MKQIFLSTMTDLEEIQTFEPGAWINLVNPSQSESMEVAEHYKID
                     IADLRAPLDAEESSRITVEDDYTLILVDVPILEERNNKTYYITIPLGIILTDDAIITT
                     CLEPLPLFEQFIHRRLRNFFTFMKSRFVFQILYRNAQLYLSALRTIDRKSEEIEKQLH
                     EATRNEELIVLMELEKTIVYFKASLKTNERLVKKLASSSRLLRKYEEDEDLLEDTLVE
                     TQQAIEMADIYGSILNSMTHAFASIISNNQNTIMKTLALVTIVLSIPTMVFSAYGMNF
                     KDNIIPFNDIPYAFWWIILFAFAISLSLTFYFIRKRWF"
     gene            16401..17057
                     /locus_tag="AC1PIW_000742"
     CDS             16401..17057
                     /locus_tag="AC1PIW_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HIP6605698.1"
                     /translation="MSFTEINGLTKKNQEFIHIATNQLIKDGKSDSEIKELLEEILPT
                     IIEKQKTGVTARNLYGAPSEWAASKTISEQEKKDQVEYNENPWLMWLDSSLFMLAIIA
                     GINGLMNLFGQGAQYGLLTLFVIGFGVGAGMYLMYHFVYREQIKTGQRPKLLKAIAFL
                     GLATLAWSVVFILAALIPAAFNPVLPPLVTILIGAAAFGARYLLKKKYNIRNAMSPVQ
                     "
     gene            complement(17447..17686)
                     /gene="rpsR"
                     /locus_tag="AC1PIW_000743"
     CDS             complement(17447..17686)
                     /gene="rpsR"
                     /locus_tag="AC1PIW_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIP6605699.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFISERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALLPFVNED"
     gene            complement(17719..18213)
                     /locus_tag="AC1PIW_000744"
     CDS             complement(17719..18213)
                     /locus_tag="AC1PIW_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIP6605700.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNQAVATFTLAVNRNFKNQNGER
                     EADFINVVIWRQQAENLANWAKKGALIGVTGRIQTRSYDNQQGQRVYVTEVVAESFQL
                     LESRTAREGQGGGYSAGNSFAGGNDYNSPYQAPAQSTPNFAREESPFGASNPMDISDD
                     DLPF"
     gene            complement(18225..18515)
                     /gene="rpsF"
                     /locus_tag="AC1PIW_000745"
     CDS             complement(18225..18515)
                     /gene="rpsF"
                     /locus_tag="AC1PIW_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIP6605701.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATIVESKAWE
                     KRRLAYEIKDFREGLYHIVNVEANNDEALKEFDRLSKINGDILRHMIVKLDA"
BASE COUNT         5379 a         4174 c         3591 g         5505 t
ORIGIN      
        1 taattttcta cacaaagata aacgtcatct ttaacctgtg ttaatatatt attttcctcg
       61 agaaaaatat ctcatgaaga aactagtaga taaagctacc caaattgtta ctaagctgtt
      121 aagtacattt tccgaagtaa ggatctcttt tttctctggc tctcgattct caattgttgc
      181 atttgaatcg accacttttt cttcctcaac agaattgtta ctacgttcaa gagaggtagg
      241 cactttcacc ttgccagcta ctgcacttct ctcttgagaa gaagttgcaa cacctacagg
      301 ctcaactgtt gaaattgctt cttcaaacaa tgtcgcagat aaagagtaag gttgaggcac
      361 cgtgtctgaa ccagcatctt gaacacgaat atctgatgtc ggaacagaaa ctgcctcatg
      421 agtatttgct tcaactccat ttggcgtagt tgcatctgct ttctcgtcta aatgttcatt
      481 tttaggaacc tctaaagtaa gtgaagcaga acttgtttgt ggagcagaaa ccagtgggtc
      541 agctacttct tgtttagcta ccaccacctc tgtagaatta gcttccggtg aagttgggga
      601 tgctgaacta ctcgcaacta cacttggttc tgttacttct gttgccactt ctgctggagc
      661 tgtttctgat gaagttggga tttcagaaat tacttgtgga gcagaaaccg gtgcttcagc
      721 tacttcttga ctagttgtta ccgtctctgt aggagttgtt tccggtgaag gttgagatgc
      781 tgaactactc tcttccgata cttctgttgc cacttctgct ggagctgttt ctgatgaagt
      841 tgggatttca gaacttactt gtggaacaga aaccgaaggt tcagccattt cttgactagt
      901 tgttactgcc tctgcagaat tagtttccgg tgaagttggg gatgctgaat tactctcaac
      961 tacacttggt tccggtgttt ctgttacctc ctctgtagga gttgtttcca atgaagtttg
     1021 aggtgctgga ttactctcaa gtgcacttaa ttccggtgct tctgttgcca cctctgtagg
     1081 agctgtttct gataaagttt ggatttcaga acttacttgt ggagcagaaa ccggtgcttt
     1141 agctacttct tgactagttg ttaccacctc ggcagaatta gtttccggtg aagtttgaga
     1201 tgctgaaatt acttgtgggg cagaaacccg tgattcagct ttttcttgac tagctgttac
     1261 cgtatctgta gaaatagttt ctgatgaagt tggggatgca gaacttactt gtggagagga
     1321 aactgtatta acctgcgctt cactcgattc tttggatgaa ggctcaacat atggtaattt
     1381 agcatcactt tcttccttag aatcaactgt attctgagct gtttctgaac tcggtgaaac
     1441 catctctgta tttcctaaag atgaagtctc tgttttatcc tctaactcag ttttagcttg
     1501 tagtgcctta ggagctgctg gtgtttgttg agaaggagca gctgagattg tttcctgtgt
     1561 tgaaggagtt ccttcaagct cctcagattg ctgtgcagaa ttaacacttg gagccgaagc
     1621 atccttggaa ttttggctga cttcatcaga attagcttgg ttttcttgca attcatttcg
     1681 gtttctatca tcatcagaat ttgtgttatt tttctgacga ttctttagat aactttgata
     1741 agtttctcct tccgaaaatt cttttccagc cttcgcattc atttgcccca agaacactcg
     1801 agctaaatcc attgaatttt cgagtgcttt aaattcttct tctgtaatct tggagagagc
     1861 actcaattta tcaatatcag aattgacaaa ttgtaaaaca cgatcatacc cctcttgttt
     1921 atcatcttta tcgtctttat ccgttgcttt cgtttcttct actttacgtt tcaaagcttt
     1981 atagatgtct agcaaatgat ttttcttatt ttctgttata taagttggct caacatatgc
     2041 aatataatcc aaaccaacca aggacttttc tttgttgaac aacagtaaat ctgctgtttt
     2101 ttgtttcata gcccaactct cataattact atcaataatc atgtagttac gctccgcctt
     2161 aggctcctgt cctgcctcaa caacatcata actatcctca ccaatagtag aaaagtgttt
     2221 ctcgctatat ttagctgagc gctgatgctt attccctgta ataaaacctg taccgtcaat
     2281 ggtgtgccac tttttatcat aataaacacg cgctgtagca tgcgggacac cttctgcatt
     2341 tttcccaaca acaacataag caggaagccc taacatatta aatgcacggg ccgcagtagt
     2401 gctgaaacct acacacatcg ctttctgacg ttcagtaacc gcgaacaaat ctgatcctaa
     2461 gtagtaattg atgtctgcat aattttgaac agccttatta gcagcttccc aatcatatgt
     2521 tgtatgattg gcaacatact gaacaaatgc cttaacttta tcgcgttcac ttgattgctt
     2581 catagcttct gaggactcta tcaacttatg aatcaagtca tcatttgcta taaaacgctc
     2641 tttggtaacg tttatatttt taactgaatt atgagtatat ttactataat cctcatagta
     2701 cttattatca actgtaagac catgcgagta aatagtcatt ccattaatat ttttcaaact
     2761 attctcagca gttcctagac ccgcagcatt taataactgc cgtttattgg agtcaagctc
     2821 aatcttaaat agtgcatgaa tccgttttgg ctccatacta ttatgtaagt cgccatttcc
     2881 cgaaaacctc aactctctca tctgattatg ataatcttcc tcattatgag caataacatc
     2941 acccttgttc ataacaagga tatgtggaaa gtcatgaata ttttgagtaa ctttaccatt
     3001 gattaccaaa aaattgttgt ttttaccaaa ttcttccaat tcttcccgag ttgataatgt
     3061 tcccagtcga ttttcaaatc ctaatttcaa aacatgaaat tgtcgttggt ccgaggagta
     3121 gtctttcaat tttttagaaa atgcttccca gttcagaacc tctattttat tggtgttttt
     3181 tggcgattgt aaatgtgagt tttcatctgc aaaaacaacc ggttgtgatg ttccgataga
     3241 tgcaagcgca agaaccgaag tagaaagggt aagtgtcaaa ataagccagc gcttaccatg
     3301 ctgttgaatt ggaatcataa gtcctccata taagtacttc tgagaataat ttcccagcct
     3361 aaaatttgca tcccaattgt aacactttta actatctttt ttctgctatc tatctgtgct
     3421 tttcctgttt ttatagactt tagtgtctcc gcatcatcac tttagatata gcgttttcat
     3481 acaattgaga aacaacaact ttcctctggt taaaaaatat cactacacta tcgccaataa
     3541 aaagattttc aaccttgctt atgaataatc atcaaaagtt gtggaactcg tttctctttt
     3601 tcatacccga tgaactccaa caatggcaaa ctctaatctt ttatagtcat cgttagtttt
     3661 tgaggcgctg tttcggctca aaaacttaaa ttgaagagtt gttgacgaac tcttttaagc
     3721 tggtcgagtt ctttcctact ccccaaaact gccttgatta acgaatggct atctcttatg
     3781 aatacagttt tttagataaa aagaagtgag agttacataa aaactctcac ttcactgtca
     3841 agttattgtg ataataaaac aacttcaccc aatattcttc aatatatcta tcgccaaagt
     3901 acaaatgaat ctgttctcat agacaattat gaatttcttg acacctgttt tgccaaagca
     3961 aagttcccaa gagttgcaga accattttca gcaacagctg gcgttacgat atattctttg
     4021 acatctggtg ttgggagata atcgttcatc aaaccgacaa acttctcacg tacacgatta
     4081 agcatgtgct cttgagccat aactcctcca ccaaatacaa ttacttgcgg acgataaagc
     4141 aaggttgcct gtactgctgc ttgagcaatg tagtaagcct gcacatccca aacgtctgaa
     4201 ttgagctcga tcaattcacc acgaatacct gtacgacctt caagagaagg acctgcaact
     4261 aagccttcca aacacccatt gtggaatgga cacattcctt taaattcatg ttttacatcg
     4321 taagggtgta gggcaacgta agtatgaccc gcttcggtat gacccaagcc accgataaac
     4381 tcaccattct gaatagcacc tgcaccaata cctgtaccga ttgtatagta aaccaagctc
     4441 tttacccctt tacgaaccag ggtttcacca aaagctgatg aattcacatc tgtcgtgaag
     4501 tagaatggaa tgttaaattc cttagcaatc agaccaagta aatcaatatt tgaccagtgt
     4561 ggttttggtg tcgaagtgat gtaaccataa gtctgtgagt tttgatcaat atcaattgga
     4621 ccgaaagaac ctatcgcaat acctgccaaa cgatcttcat agcgcttgaa gaattcaact
     4681 gttctctcaa tcgtttcata aggagttgtc gttggaaatt gcgttttttc aacaacttga
     4741 aagttctcat cacctactgc acaaacaaac tttgtaccac cagcttctaa gctaccatat
     4801 aattttgtca tttgattgtc tccaaaattt ttatcacttt tattataaca tataagcaaa
     4861 cagaagaaaa aggctgaaag cctttttcct gttaattatg tagtagagtc atgctctaca
     4921 aatcgatttt atataagatt acttagctac tttcaagaat tctacaccgt gagcgattgt
     4981 tccctctgca agtggagata cttctgcaaa gtcagcagtg ttggtcacga tgatcatggt
     5041 tgtatcatca agacctgctt cagcaatctt agctgcatca aatttagcaa gcggagtacc
     5101 agcttttact ttatcacctg ctgctactaa tttttcaaat ccattaccat tcattgatac
     5161 agtatcaata ccgatgtgaa ttaaaagttc agcaccagac gctgttttca atccgtaagc
     5221 atggcctgtt tcaaaagcaa ttgttacttc tgcatctgct ggagcgtaaa caacaccctc
     5281 agatggttta acagccaaac ctttacccat agctcctgat gagaagactg ggtcattaac
     5341 attttcaagg gcaacaacat cacctgaaag tggagataca agagtttctt cgttgagctc
     5401 aacagctgga gttgcaggtg ttgctactgg agctgccgct gctttaggtg cagcttctac
     5461 ttcatcttca aaaccgaaga ggtaagtcaa tgcaaaacca agagcaaatg atacagctac
     5521 cataaggatg tattggaaca attgaccatt accaacataa agtgtcgcac caggaatgat
     5581 ggttacaccc atacctttac cagcaagacc aagaagagat gctacagcac caccgatggc
     5641 accagcaatc aatgaaagaa gaaatggttt gcggaaacgc aagttcacac cgaagatagc
     5701 tggctcagta ataccgagga aggcagaaag agctgctggg aaagcaagag ctttcaattt
     5761 aggattttta gttttcacac caaccgctac tgtagcagca ccttgtgctg tcatagctgc
     5821 agtgatgata gcgttgaatg ggttagcacc agtgtttgct accaattgag cttccaagaa
     5881 gttgaagata tggtgaacac ctgatacaac gataacttgg tgaacaccac cgataaggag
     5941 accaccaagc cccattggca agttcaagat agcttgtgta ccagctaaga tgtagttttc
     6001 aaccacgtgg aagactggac cgatgacaaa caaaccaaga atagacatga caaacaaagt
     6061 cacgaatggt gttactaaga ggtcaagaac ttctggaaca accttgcgga ctgctttttc
     6121 aaatttagca ccaaccaaac cgatgatgaa ggctggcaat actgaacctt gcaaaccaac
     6181 aactgggatg aaaccgaaga aaatggttgg cttcacatca ccacctgaag caacgaccca
     6241 agcatttggc aattgaccag caatcagcat cataccaagt acgataccga tagtttggtt
     6301 cccaccgaat acgcggaagg ttgaccatac caccaaggct ggcaagacga tgaaggctgt
     6361 atcagtcaag atttgtgagt aaacgttcaa atcttctgga agagtatagc cgagagctcc
     6421 aagaagacca cgaagaccca tgaaaagacc agttgctacg atagcaggaa tgattggaac
     6481 gaatacatca ccgaaagtac ggatagcacg ttggaatgca ttgccttgtt tagcagcttc
     6541 agctttttgt tcaccagtag tcgctgttgg caaaccaagg gcaaccactt catcgtagat
     6601 tttgtttact gtaccagtac caaagatgat ttggtattga ccagagttga agaaagcacc
     6661 ttgtactttt tcgatgtctt cgactgtatc cttgtcgatt tttccttcat caacaaccat
     6721 tacacggagg cgagtcgcac agtgagcaac actgcgtacg ttctcacgtc caccaagggc
     6781 gtcaatgact ttttttgcaa tttctgtatt attcatcttg caaaaatctc cttattaaat
     6841 attttttgtt ttttgaaagt gttttcaaac tttcgatggt tttattatat cacttttata
     6901 aaatatgtca aacgtttatc acattttgtt tcaattttat caaaaaacat tgttaaaact
     6961 tgatttttcg caagaaaacg tttactatat taatgaaaaa ctagaaaatt tggagaaaat
     7021 tatggcattt acaacagggg agcgttacag agcttatgct gactggacac ccgaatacat
     7081 tgaaaaaata aagaagaata cagagagttc tccttggagg acaaatttcc atattgaaac
     7141 accacatgga ctgttaaatg atccaaatgg attttcttat tttaatggaa agtggactct
     7201 tttttaccaa tacttcccat tcggtgctgc gcacggattg aaatcgtggg tccatacaga
     7261 atcaacagat ttggtccatt ttgaagaaac tggtacggtt atgtacccag atacaccact
     7321 tgatagccat ggtgcctact ctggatctgc catggaattt ggcgacaagc tcttcctctt
     7381 ctacactgga aatgtccgcg atgaaaattg ggttcgccat ccataccaaa tcggtgcctt
     7441 gatggataaa gatggtaaga ttgaaaagat tgataaagtc ttgattgagc aaccagaaga
     7501 taccaccgac catttccgtg atccacaaat tttcaactat aaagggcaat attacgctat
     7561 tgtcggtgga caaaatctgg ataaaaaggg aattgtcaaa ctttataaag ctgaaaataa
     7621 cgactacctc aactggtcct atgttggtga ccttgatttt gccaatgata tgaccgctta
     7681 tatgatggaa tgcccaaata ttgcgttcgt cggtgagcaa cccatccttc tctattgtcc
     7741 acaaggtttg gacaaagagg tattggacta tggcaatatc tatccaaata tgtacaagat
     7801 tggtcagagt ttcgttcctg aaacagcaag cattgtcgaa ccgagcgaat tgatcaatct
     7861 ggactatggt tttgagtgct atgcaaccca ggcctttaat gcaccagacg gtcgaactct
     7921 atctgttagc tggttggcac ttccagatgt ggaataccca accgatgctt atgactacca
     7981 aggctgtctg tcattggtta aagaattaac catcaaggac ggtaagctct accaatatcc
     8041 agtcgatgcg attaccagtc ttcgtaaaga agctcagcca ttttcagccc aaaaagaaac
     8101 caataatgtt tacgaattgg aacttgattt ttcagcgagc acgaagcacg aaatcgtatt
     8161 gttcgcaaat gaagcagaaa aaggcttggt cttgtctgta gacactgaac aaggccagat
     8221 tacattggac cgtggcaact gtggtcagcc attcgcactc gatttcggca caagtcgctc
     8281 ttgcgagatt tctccaggag ctcttacagc caatatcttc atcgacaaat ctgtctttga
     8341 aatctttatc aataaaggag agaaagtatt ttctggtcgt gtcttccctg atgcggatca
     8401 gtctggtata gtcattacga ctggtaatcc gaccggaacc tactaccatt tagattatgg
     8461 tcgcaaaact aactgatgtc gcacagttag ctggggtcag cccaacaact gtctcgcgcg
     8521 ttatcaataa aaaaggatac ctatctgaaa aaaccattcg caatgtcgaa gaggctatgc
     8581 gtgaactagg ctacaaacct aataatttag ctcgtagttt gcaaggtaaa tctgcaaaat
     8641 tagttggtct catcttccca acaataaaca atatttttta ttctgaattg attagccatt
     8701 tagaaaaaga gctttttgat cgaggctata aaaccattat ttgcaatagc cagcacgagt
     8761 ccgataagga acgtgagtat ctagaaatgt tagcagctaa ccaagtagat gggattatct
     8821 cgggcagtca caatctcggt atccaagact acgaccgtgt cgtagctcca attatcgctt
     8881 ttgaccgcaa cctatctcct tccattccca ttgtctcttc cgataatttt gctggcgggg
     8941 tattagctgc ccaaacctta caaaaagcgg gatgtcacaa tccattaatg attacgggga
     9001 acgatgattc caattcacct accggccttc gccaagtagg ttttacatct attttgaaca
     9061 aagccgaagt ttttcatatc tccagtgatt tctcacctgt gcgtaaggag atggaaatcc
     9121 gagccattct tgaaaagcaa aaaccaaatg gtatctttgt atctggcgat gcaacggcta
     9181 tgctggtatg gaacgtcatt cgttcaatga aactgtctat tccagaagat attaaattga
     9241 ttggttatga tggaaccagt ttcatcgaaa actactaccc tcaactgaca actatcaagc
     9301 aaccattaac cgaaatagct cgcctgctgg ttgaactatt agttgataaa atcgaaggaa
     9361 aaaaactgac aaaaacagac tatatcctgc cgattagcct actagcagga gctagtatat
     9421 aaaaaaagct gtacctaata aaaattaggt gcagcttttt tgtgatacat tctcttattt
     9481 ctcaagtaaa aaagaagctc atcaggcttc ttctttaata aactggctca aaactccatt
     9541 gacaaactta gctgaagatt catcagaaaa ttctttagcc aattcaatag cctcgttaac
     9601 agcaacgcgg tctggcgttt cttcgaaatg taagatttca aataggccta gtcgcatgat
     9661 attcttgtca atcaaggtta aacggtctaa ggtccatcct gcttttaatc ggctagataa
     9721 ctccttatcc aagtcatcac gatagtctgc tacaccattg acaaggttaa ggagaaagag
     9781 cggaatttca actccttctt gctcttcttc tctatcataa agataagaaa actgagctgc
     9841 ctgaacaggt tcttgaccaa attcaagtga tacgattgcc tgcaaagcac acttacgcag
     9901 ggcatgtcta ttattcttca tcaaggaaat cctctccgaa taaatctttc aatgctggtt
     9961 ttggtgtttt atcaggaaca atgccgctga catggatatt aaccgcagcc acttgaactt
    10021 ctgcatagtt ataaactgct gtcttgactg cacgttggat gtccattgat acggcaggaa
    10081 catttacacc atattctaga tagacgtaaa tatccgcagt cactcgacct tcctcgtcag
    10141 tttcaagata aacaccttta tttaacgatg tttttgacca actgtctgct acacgcttat
    10201 tttgaaggga atgcactcca tcaactttcg ctgcagcaat gcctgtaatt acttcaagga
    10261 cgcgaggtgc gataacaatc tcgccttgat attctgttgt cataggctct cctttcttaa
    10321 gaggaaacga ttaagcacgt gatacgtaag ttccttcagc tgtgttgata acgagttttt
    10381 gacctgcttc gataaagtct ggtacgttga ctacaagacc tgtttccatc gtagctggtt
    10441 tacctgaacc tgtaacagtc gcacctttga tagatggttg tgtttctgca acgaccaatt
    10501 caacagttgt tggtacagta acaccgatca cttctgttcc gtagaactgg atcttcactt
    10561 cagaattctc caagatgtag agcaattctt ctttgatagt cgctactgga atttcatact
    10621 gatcgtaagt ctcagtattc ataaaataag ctgtgtcatc catttggtaa aggtactgtg
    10681 ctggaactgt ttcgataata gcttgttcaa atttttcatc tggacggtaa gttgtatcga
    10741 aggttgaacc tgtacgtaca tcacgcaatt tcatacgcat gatggtgtta cctttacctg
    10801 gtttgtggtg gctcgcatcc aaaactttaa tcaatttacc ttcagcttcg aaggtcatac
    10861 ctgctctaag tttacttgct tcaatcattg tattgaatac ctcttcaaat tttatttatt
    10921 cgttttattc taccacaaac tgtcaaattt ctcaaatgac aatcaattcc ttgggagcta
    10981 atgtcaagac ttcacaaccg ttttccgtaa tcaataggtc atcttcgata cggacaccat
    11041 atttgtcatc caaatagata ccaggctcat ccgtcaaaac cataccggcc ttgatagtct
    11101 cgtcagattt tccaaaatac ggatattcat gaatatccaa gccaataccg tgaccgatgc
    11161 cgtgtgtaaa gttggctccg taacccgccg cactgataat ctcacgagga atagcgtcaa
    11221 actcgcggta ggtgacacct tccttggcct gctcaatcaa agccttattg gcacgcaaga
    11281 ccacatcgta aatctcacgc tcttgatctg tcacatgacc gatatggatg gtccgtgtca
    11341 tatcgctgac ataatgctgg taatagcaac caaaatccag cgtcaaggtc tcaccagatt
    11401 ggataacctt ttcagacgct cttccatgag gcatagcaga gcggtagcca gatgccacga
    11461 tgaactcaaa tgatgcccct tctgcaccga gttgacgcat gcggtggtca aggaaatgat
    11521 tgacgtccat ctcagtcgtc tgacctggtt tgataaagtc aagaacatca ataaaagcct
    11581 gatccgaaat ttgacatgca cggcgaatgg tcgcaatttc tttctcatcc ttaatcatgc
    11641 gcaagtcttc gataaagttt gacatagcaa ccagttgata accctcaaaa atgctggtca
    11701 gactttgata gaaaccatag gtcacctcgc tatcaaaacc aattttttca agcccatctt
    11761 ccttaatcac ctttgcaatt tcttcaaggg caaaacggct ctcgataata tcaaaacctt
    11821 ttactgatgc cttggctatc aaggtataac gcgaatcagt cacaaacaag cgacgcttgc
    11881 cactaatgaa gacagtcgcc tctgtccccc aaaaacctgt caggtagtaa atattgtttt
    11941 gaccagttac caagattcca tcaacagttg attgagctaa ttttgcttca aatttttcaa
    12001 gtcttttttg catcctattc ctccaatgat tttttaacta ttatagcaga aatacagaca
    12061 aataccaatt cttcctagca gatagagaaa aaaagccgca gaggcgactt tttatttcaa
    12121 cttcattttc agatactggc ctgtaaagct ggctgggttc tccgccactt cttctggtgt
    12181 tcctgtcgcg acaacggtac cgccaccgac accgccttcc ggtcccatgt cgataatgtg
    12241 gtcggctgtc ttgataacat ccagattgtg ctcgatgacc agcacggtat tgccatcatc
    12301 aacaaagcga gccaagacct tgagcaactg ggcaatgtcc tccgtatgca gacctgtcgt
    12361 cggctcatcc aagatataca aggatttacc agttgaccgc ttgtggagtt cagaagccaa
    12421 cttcatacgc tgggcttccc cacctgatag ggtcgttgct ggctgaccta gagtaacgta
    12481 gcccaaacct acatcttgaa tagtacgaag tttccgctca atttttggaa tatgcttgaa
    12541 gaactcgacc gcgtcattga ccgtcatatc aaggacctga gcgatatttt tctccttgta
    12601 gtgtacttcc aaggtttcag aattatagcg gtgtccatga cagacttcac aaggtacaaa
    12661 gacatcgggc aggaagtgca tttcaatctt gataatccca tcaccagaac aagcctcaca
    12721 ccgtccacct ttgacgttaa aggagaaacg gcccttcttg tagccacgaa tcttggcttc
    12781 attggtctgg gcaaacaaat cccgaatatc gtcaaagaca cctgtgtagg tagctgggtt
    12841 agaacgtggt gtccgtccaa tcgggctttg gtcaatatca atcaggcggt ccaagtgttc
    12901 aattcctgaa atagacttga acttacctgg cttgtccgaa ttgcgattga gtttctgagc
    12961 aatagctttt ttcagaatgg aattgaccag agttgactta cctgagccag acacccctgt
    13021 caccgctaca aattttccga gtgggaaacg aacagtcaca tcttgcaggt tgttttcctt
    13081 ggcaccggtc acctctaaga atcgaccatt tccaacacga cgttccaaag gaactggaat
    13141 ttcacgtttg ccagacaggt actggcctgt aatggatttc ttgtttttag ctacttgggc
    13201 tggtgtgcca gaggccacga tttctccgcc aaacacaccc gcacctggac cgatgtcaat
    13261 caaccagtcc gcctcacgca tagtgtcctc atcatgctcc accacaatca atgtattgcc
    13321 caaatcacgc attttcttaa gactagcaat cagacggtca ttgtcccgct ggtgcagacc
    13381 aatcgacggc tcatccagaa tatagaggac accagacagg ttagagccaa tctgagtcgc
    13441 cagacgaatc cgctggctct caccaccaga aagggtacct gctgctcgtg aaagagtcag
    13501 atagttgaga ccaacgttat ttaggaagga caggcgatcc ttgatttcct tgacaatggg
    13561 tgttgcaatg gtggcttcat tttcagacaa ggtcagatgt tcaatgactt gcaaatgatc
    13621 cgccacggac aagtctgata attgtccgat atgaagtccc tgctcgcctc cgactttgac
    13681 agacagggct tggtcattga ggcgatagcc gtggcaggtc ccacaggtca gttcattcat
    13741 gtaagtcttc atgactgtcc gtgtgtaatc actattggtt tcacgatagc ggcgattaat
    13801 gttggtaatc aagccctcga atggaatgtc aatatctcgg actccaccga actcattttc
    13861 ataatggaaa tggaattctt tcccgtcaga accgttgaaa atcaggttct tctcctcttc
    13921 cgtcaattcc tcaaaaggct tgtccatgtc cacaccaaag tgattcatgg cctgctccaa
    13981 catctgagga tagtagttgg atgagattgg attccaagga gccaaggcac cttcacgcaa
    14041 ggtcttgctg gcatctggga caatcaaatc cgtatccact tccagcttca tgcccaaacc
    14101 gtcacagtcg ctacaagacc caaaaggagc attgaaagag aagaggcgag gctccaactc
    14161 tggtaccgta aaaccacaaa ctgggcaggc atagtactca gagaaaagca tttccttctc
    14221 atccatggtg tcgatgatga catagccgtc cgcaatccgc agggccgcct caatggagtc
    14281 aaaaagccgc gagcgaatgc catctttgat cacaatccga tcgaccacaa cttcaatatt
    14341 atgagccttg ctcttggaca attctggtac ttccgacaca tcatagacac cgccattgac
    14401 acgtacccga acatagccgt ccttctggat tttctcaaaa atggtcttgt gttgcccctt
    14461 tttcttacga acaatcggag ctagaatctg caaacgttgg cgttctggca attccaagac
    14521 ctcatcaacg atttgttcca ccgaagaagc cgcaatcgct ccgtggccat tgatacagta
    14581 aggtacccca acccgagcat aaagcaagcg gagatagtca ttgatttccg tcgctgtacc
    14641 gactgtcgaa cgcggattcc gcgatgtggt tttttggtca atggaaatag cagggctgag
    14701 accctctatg gaatccacat caggcttgtc catgttgccc aagaactgac gagcataggc
    14761 agacaaagac tccacatagc gacgttgacc ctcggcatac aaggtatcga aagccaaact
    14821 cgacttacca gaccccgaca aaccagtcac caccaccagc ttctcacgcg gaatggtcac
    14881 atcaatattt ttcaaattat gggcacgcgc cccatgaatc acaatatttt cttgcataac
    14941 atgatataag tgcgttaaga aagcgataag aaaataggaa aatgtgacgt cacatcagtg
    15001 acaaaacatt ttgtcttttt ctcgacgctt ttagcacgca ttcgaatcct ttctaagata
    15061 taaagagcat caaatgcaca gtcaaaatag gcggtaagtc cgaattctgt gatttcgaag
    15121 atgcaccccc ttcaagacaa ctagtgcttt cacaggttga aaaccgaaga aagtacagat
    15181 gtctacgacg cagaaaccag ttcccatact tatccatgaa gaaactagca ctttttgaca
    15241 ccgcatttag cccgtgttca attcctttct taagaggcta taagctatgg tatacggaac
    15301 aaggaaaagt ccgtacaact tattcgctta aatgtctaca tattatatca catttctata
    15361 taaaaccttt ccgaaactgc acattttttg gtataataga ggttgcgtta acgcaacaaa
    15421 gataaaagga gggcaaagat atgaaacaaa tttttctatc aaccatgact gatttagaag
    15481 aaatccaaac ctttgaacca ggggcttgga ttaatttggt caacccctcc cagagcgagt
    15541 ctatggaagt ggctgagcac tataaaattg acatcgctga cctgcgagca ccgctcgatg
    15601 cggaagaatc ctctcgtatc accgtcgaag atgactatac gctcatcctg gtcgacgtcc
    15661 caattcttga agaacggaac aacaagacat attatattac tattcctttg ggaattattc
    15721 taacagatga tgcaattatc acgacttgtt tggaaccatt gccactgttt gagcagttta
    15781 tccatcgtcg tctacggaat ttcttcactt tcatgaagtc tcgtttcgtt ttccaaattc
    15841 tctaccgaaa tgctcaactc tacttgtcag ccctacggac cattgacaga aagagcgaag
    15901 agattgagaa gcaactccat gaagccactc gaaatgaaga attaattgta ctcatggaat
    15961 tagaaaaaac catcgtctac ttcaaggctt ccctcaaaac caatgagcgt ttggtcaaaa
    16021 aacttgctag ttcctcacgc ctactccgta agtatgaaga ggatgaggat ttgcttgaag
    16081 ataccttggt cgaaacccaa caggccattg agatggcaga catctatggt tccatcctga
    16141 actctatgac ccatgccttt gcttctatca tttctaacaa ccagaatacc atcatgaaaa
    16201 ccttggcatt agtgacaatc gttctctcta tcccaaccat ggtcttctct gcatacggta
    16261 tgaactttaa ggacaacatc attcctttta acgatattcc ctatgctttt tggtggatta
    16321 tcctcttcgc atttgcaatc agcctatcac tcaccttcta cttcatccgt aagagatggt
    16381 tttaaactaa ggagattaac atgtcattta cagaaatcaa cggattaaca aaaaagaacc
    16441 aagaatttat ccatattgcg accaaccaac tgattaaaga tggaaagtct gatagcgaaa
    16501 tcaaagaact cttggaagaa attttaccaa ctatcattga aaagcaaaaa actggtgtga
    16561 ctgcccgcaa tctctatgga gcaccaagtg agtgggctgc tagtaagaca atttctgaac
    16621 aggaaaagaa agaccaagtg gaatacaatg aaaatccatg gttgatgtgg ctagattcta
    16681 gtcttttcat gcttgcaatt attgctggta tcaatggttt gatgaatctt tttggtcaag
    16741 gtgcacagta tgggctatta accttatttg tcatcggttt cggtgttggt gcagggatgt
    16801 acttgatgta ccatttcgtt tacagagaac aaattaaaac tgggcaacgt cctaaattgc
    16861 tgaaagctat cgcttttctc ggacttgcta cgctggcttg gtcagtcgtt ttcattctgg
    16921 cagcactgat tccggccgca tttaacccgg ttcttcctcc gcttgttact attcttatcg
    16981 gagctgctgc tttcggtgca cgctacctct tgaaaaagaa atataacatt cgcaatgcca
    17041 tgtccccagt tcaataataa aacaaagcga ttggaatatt ccagtcgctt ttgtttatct
    17101 atacagttca gctatcaaat cgcccgagaa ccttatccgc tgtcacttgt ctatgtaatc
    17161 tgctgaaaca gtctatcccc agactgtgcc tcgctttcta acttcttggc tcgggttgaa
    17221 acagtccatt caaaggctta attcgctttc ttaatttcga gttcatgaaa aaatggtcca
    17281 ctctcgccgt tgcgcctttc cagtttcttg ggcgcaagct aaaaagaccc gtcggggtct
    17341 caattcgctt tcttattttc atgctcatga aaaaacagtc cattctcgcc gttgcgcctt
    17401 tccagtttct tgggcgcaag aaaaaatggt ccactggacc atttttttaa tcttcgttta
    17461 cgaatggtaa aagagccata acgcgagcac gtttgatagc tgttgttact ttacgttggt
    17521 ttttagctga agttccagtt acacggcgtg gaaggatttt tccacgttca gaaatgaaac
    17581 ggctaagaag ctcagtatct ttgtaatcaa catattcaat tttgttagct gcgatatagt
    17641 caactttttt acggcgtttg aaaccgccac gacgttgttg agccatgtgt gttctccttt
    17701 atattattaa tttttgacct agaatggtag gtcatcgtct gatatatcca ttggattact
    17761 tgctccaaat ggactttctt ctcgagcgaa gtttggtgta gattgtgcag gcgcttgata
    17821 aggcgagtta tagtcatttc ctccagcaaa cgagttgcca gctgaatagc ctccaccttg
    17881 accttcacgg gcagtacggc tttccaagag ttggaaactt tctgcaacta cctcagtaac
    17941 gtagacacgt tgcccttgct gattgtcata gctacgagtc tgaatacgac ctgtaacacc
    18001 aatcagagca cctttcttag cccaattcgc caaattctca gcttgttgac gccaaatgac
    18061 tacgttgata aagtccgctt cacgctcacc gttttgattt ttaaaattgc ggttaaccgc
    18121 caaagtaaaa gtcgcaacag cttgattaga cggagtataa cgaagttctg catcacgggt
    18181 catacgacca accaatacta cattattaat catacaacac cttcttacgc gtcaagtttg
    18241 acaatcatgt gacgaagaat gtcgccgttg atttttgaca aacggtcaaa ctctttaaga
    18301 gcttcatcgt tgttagcttc aacgttaacg atgtggtaca aaccttcgcg gaaatctttg
    18361 atttcgtatg caaggcgacg tttttcccat gcttttgatt caacgatagt tgcaccgttg
    18421 tcagtcaaga tagagtcaaa gcgtgctacc aaagcgtttt tagcttcttc ttcaatgttt
    18481 ggacgaataa tataaagaat ttcgtattta gccattgata tgttcctcct tttggtctaa
    18541 tgactcatgg tctagccacg agtaagtgag ggatactcac agaaaataat tataccataa
    18601 tatagaaaat gtgcaagtga aaacaatttt ccgaaaacat tttcacaaa
//
LOCUS       DBNFCK010000027        18429 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.027, whole genome shotgun sequence.
ACCESSION   DBNFCK010000027 DBNFCK010000000
VERSION     DBNFCK010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 18429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18429
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.027"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(16..927)
                     /locus_tag="AC1PIW_000746"
     CDS             complement(16..927)
                     /locus_tag="AC1PIW_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIP6605702.1"
                     /translation="MRIQQLYYIIKIAETGSMNEAAKQLFITQPSLSNAVRDLEKEMK
                     IKIFFRNSKGITLTKDGMEFLSYARQIVEQTELLEDRYKNPNAKRQLFSVSSQHYAFV
                     VNAFVSLLKETEMEDYELFLRETRTWEIIDDVKNFRSEIGVLFLNHFNRDVLLKLLDD
                     NRLSYTTLFTAKPHVFVSKTNPLAQRKSITLDDLVDFPYLSYEQGIHNSFYFAEEILA
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLEFDDEIELVYI
                     QHEKALLSDMGEKFINYLLEEVKFDQK"
     gene            1141..1914
                     /locus_tag="AC1PIW_000747"
     CDS             1141..1914
                     /locus_tag="AC1PIW_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HIP6605703.1"
                     /translation="MILKEQMLLVDQVQLAPRIFAMTLQGDMVQDMKMGQFIHIRVPD
                     DSMLLRRPISISEIDRNKSQCRIIYRVEGQGTEVFSKMTVGQYLDVMGPLGKEFEVDF
                     LKEGDRILIIGGGIGVPPLVEVAKQAAFRGVNVTSVIGFATKEAVILEEELASYGMVY
                     VTTDDGSYGRKGNVATVVEELTNEFAAIYSCGAPAMMNYVDQRFQEHPHAYISLEARM
                     ACGMGACYACVVKPKEGQEHENKRVCKEGPVFATGSLIL"
     gene            1924..2865
                     /locus_tag="AC1PIW_000748"
     CDS             1924..2865
                     /locus_tag="AC1PIW_000748"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HIP6605704.1"
                     /translation="MTKRLAISLPGLDLKNPIIPASGCFGFGQEYADYYDLNQLGSIM
                     IKATTRHPRYGNATPRVAETPAGMLNAIGLQNPGVEAVLSEKLPWLEQHFPDLPIIAN
                     VAGFSNEEYAYVSGKISKAPNVKAIELNISCPNVDHGNNGLLIGQVPELAYQAVKAAV
                     EASSVPVYVKLTPSVADITLLAKAAEAAGATGLTMINTLVGMRFNLKTRQPILANGTG
                     GMSGPAVFPVALKLIRQVAQMTDLPIIGMGGVDTADKAIEMMVAGASAIGVGTANFTD
                     PFACPKIIQDLPKRMEVYGIDSLEGLRREIRYSLGKR"
     gene            3030..3725
                     /gene="pyrF"
                     /locus_tag="AC1PIW_000749"
     CDS             3030..3725
                     /gene="pyrF"
                     /locus_tag="AC1PIW_000749"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HIP6605705.1"
                     /translation="MKETRPIIALDFAGKEEVQSFLEQFPPDEKLYVKVGMELYYAEG
                     PGIINYLKDCGHSIFLDLKLHDIPNTVESAMKVLAKLGVDMTNVHAAGGVEMMRAARR
                     GLGKDAVLIAVTQLTSTSEEQMRTDQNIQTSLQEAVVHYAQRAQEAGLDGVVCSAHEV
                     ALIKDATSSDFVCLTPGIRPTGGEVGDQKRVMTPADAARIGSDYIVVGRPITKSEHPY
                     QTYLSIKEEWNRG"
     gene            3718..4044
                     /locus_tag="AC1PIW_000750"
     CDS             3718..4044
                     /locus_tag="AC1PIW_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HIP6605706.1"
                     /translation="MDKIYQVIIDRPLGYIDKFGNQYPINYGYVPNLLAGDGEEQDVY
                     IISKAVRQPLTAFEGKLVAIIHRRDDIEDKWVLTGLDEKLTVTEIKEKTNFIDQYFDS
                     WIEMVD"
     gene            4182..4814
                     /gene="pyrE"
                     /locus_tag="AC1PIW_000751"
     CDS             4182..4814
                     /gene="pyrE"
                     /locus_tag="AC1PIW_000751"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIP6605707.1"
                     /translation="MTLATEIASHLLDIKAVHLRLEKPFTWASGIKSPIYTDNRITLS
                     YPETRNLIEDGFVQRIKEEFSEVEVIAGTATAGIPHGAIIADRMNLPFAYIRSKPKDH
                     GAGNQLEGRIVKGQKMVVVEDLISTGGSVLDAVAAAEREGADVIGVVAIFTYELPKAD
                     RNFDNAGVKLVTLSNYSELIKVAKVQGYINADGLNLLKKFRQNQETWQED"
     gene            4923..5849
                     /locus_tag="AC1PIW_000752"
     CDS             4923..5849
                     /locus_tag="AC1PIW_000752"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIP6605708.1"
                     /translation="MISVIVPCFNEEEAIPYFYDAMEKVRKEMGEQFEYIFVNDGSKD
                     GTLTVLRQLSGQDRAVRYLSFSRNFGKEAALYAGLQAAQGELVTVMDVDLQDPPEMLM
                     EMKAMLDGNPDLDCVGTRRVSRDGEPPIRSFFAKLFYKLMNKISQVEVVDGARDFRLM
                     RRHMVDAILSVSEYNRFSKGIFAWVGFETEYLPYENVERVAGETSWSFWKLLSYSIEG
                     IINFSDTPLNIASYTGFFTFLLSLVLMVFVVFKTLVFGDPTIGWPSTICIILFLGGLQ
                     LMTIGILGKYLAKVFLETKKRPIYIVKEKSSN"
     gene            5859..6512
                     /locus_tag="AC1PIW_000753"
     CDS             5859..6512
                     /locus_tag="AC1PIW_000753"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIP6605709.1"
                     /translation="MEHSAWHELIKAQLPEHYFGKINQFLDHVYAGGTIYPPREKVFA
                     AIQTTDLEEVKVVILGQDPYHGPGQAQGLSFSVPNSVPAPPSLQNILKELTDDIGVKQ
                     DHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIKVVNSLDQPVVYILWG
                     AYARKKKALITNPKHLVIESAHPSPLSAYRGFFGSKPFSQANAYLTSQGRTGIDWLR"
     gene            6552..7823
                     /locus_tag="AC1PIW_000754"
     CDS             6552..7823
                     /locus_tag="AC1PIW_000754"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIP6605710.1"
                     /translation="MLLVKNGRVLDPQSGLDKVCDILMDGSKIVKIAESISEDVEQEV
                     IDATGLVVAPGLVDVHVHFREPGQTHKEDIHTGALSAAAGGFTSVVMMANTSPTISNI
                     ETLKEVLDSAKKEAIHIYSVATITDHFDGQNLTDFPALLEAGAVAFSDDGIPLTNAGV
                     VRKAMKLAKEQGCLISLHEEDPDLNGVLGLNEQVAEKHFHVCGATGVAEYSMVARDVM
                     IAYETGARVHIQHLSKAESVKVVEFVQGLGANVTAEVAPQHFSRTEELLLTKGANAKM
                     NPPLRLESDRLAVIDGLKSGVISVIATDHAPHHPDEKNVEDLTQAPSGMTGLETSLSL
                     GLTHLVEEGHLTLVQLLEKMTINPAKLYGLDAGYLAENGPADLVIFDPNANRLVTADF
                     ASKSANSPFVGDSLKGQVLYTIANGQIVFGK"
     gene            7954..10098
                     /gene="nt5e"
                     /locus_tag="AC1PIW_000755"
     CDS             7954..10098
                     /gene="nt5e"
                     /locus_tag="AC1PIW_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface ecto-5'-nucleotidase Nt5e"
                     /protein_id="HIP6605711.1"
                     /translation="MKKKKILLPVMSTLLLAPFVLAQQVQAAETTTAATTTNQPATTD
                     ATATVPATTDATATVPATSVENVATEETVVPAAEETVEAVIIHTNDVHGRILEEKNVI
                     GDAKAAAVIEEERAKVENTIVVDAGDAFQGLPISNSTKGEDRANIMNQVGYDAMAVGN
                     HEFDFGMDQAIKYKETLNFPLLSANTYVNGARVFEASTIVDKTPTVVGDEFVVIGVTT
                     PETATKTHPKNVEGVTFTDPVTEVNKVIDEVEARALADNRVYKNYIILAHLGVDSTTP
                     VEWRGSTLAEALSKNSKLAGKRVIVIDGHSHTVEATTYGDNVTYNQTGSYLNNIGKVT
                     LKSDKLLGEASLISAADTKNVTPNAKIAALVDEIKAKYEAENAQVVIENNPVELNGDR
                     SNVRVRETNLGNAVTDAIYAYGQTGFSNKTSLAVTNGGGLRATIAKDQPVTKGDIIAV
                     LPFGNIVSQITVTGQQIYDMFTKSLSSTLQVNPETGEMLLDENGMPLFEASGGFLHIS
                     GANVFYDPTLPVEERVLLIGILNPETGEYDALDLEKTYYLATNDFLAAGGDGYTMLGG
                     AREEGPSMDSVFAEYLKTADLSAYEVVNPYSRIIPVNSSIDTDEDGYPDFIEILLDTD
                     PENPASNPETVPAENTDSPSNQVQNTSATDKKAPVDSPKVGDKKTEVASPAKTTKAGV
                     LPNTGDQMNLTLSLFGLGLAGLAVAVGRRKEN"
     gene            10150..10797
                     /locus_tag="AC1PIW_000756"
     CDS             10150..10797
                     /locus_tag="AC1PIW_000756"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIP6605712.1"
                     /translation="MQTVFQYQKEGDCEQISSYVLLSNSPRRKQLLGFLQPQIASVEV
                     DERGIEEHFMKQFATDDFLTRAAKTCCEISKAKSDMDLVTGHLYISADTIVVSDGQIF
                     NKPQDLTEAETMLRSYFGKSHHVVTSVCLRTRHSLDVFYTVAQIDFVDYYPALEEVIQ
                     SYIREKQPLDKAGAYGIQELDPRFVAGISGDIHTIIGLPVAEVSCRIFSEEGFEN"
     gene            10849..11337
                     /locus_tag="AC1PIW_000757"
     CDS             10849..11337
                     /locus_tag="AC1PIW_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HIP6605713.1"
                     /translation="MTVDIERYKQLAQQKQGEHRKFLANLKKKTPKNLDRIVQDIHAE
                     VFSEIDCTECANCCKTLGPLFTEADITRIAKHLRMKLPVFEDMYLRMDEDGDKVFQSM
                     PCPFLGGDNLCSIYDARPKACREFPHTDRKKIYQINNLTIKNTLICPAAYLFVEKLRE
                     RI"
     gene            11437..12288
                     /gene="ylqF"
                     /locus_tag="AC1PIW_000758"
     CDS             11437..12288
                     /gene="ylqF"
                     /locus_tag="AC1PIW_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002962311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HIP6605714.1"
                     /translation="MAIIQWFPGHMSKARRQVQENIKHVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLMILNKVDLADPVRTKEWQSYFEGQGIRTLAINSKEQATVKKVTDAAK
                     GLMKDKIERLRERGIQKETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKSNKDLEILDTPGILWPKFEDQEVGLKLALTGAIKDQLLPMDEVTIFGLDYFKANY
                     PERLQERFKGMDLEQETPELIMDWTKKLGFRDDYDRFYNLFVKDVRDGRLGTYTLDRV
                     SDLDGND"
     gene            12275..13048
                     /locus_tag="AC1PIW_000759"
     CDS             12275..13048
                     /locus_tag="AC1PIW_000759"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIP6605715.1"
                     /translation="MATIKEVTALLAQVDSLDSPVWDQLAQDERAGVQAAIKKRRKEL
                     EKEAVEDARLEAMLFYEKSLYENGVEFIAGIDEVGRGPLAGPVVAAAVILPKGCKIRY
                     LNDSKKIPKSKHEAIYQEVMERAVAVGVGIKDAALIDEVNIYEATKLAMLEALGKLSQ
                     KPDHLLIDAMKLDTPIPQTSIIKGDANSLSIAAASIVAKVTRDKMMADYDKEFSGYGF
                     AKNAGYGTTEHLEGLNKLGITPIHRKTFEPIKSMVAGGN"
     gene            13051..14061
                     /locus_tag="AC1PIW_000760"
     CDS             13051..14061
                     /locus_tag="AC1PIW_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HIP6605716.1"
                     /translation="MTVFFWLFLAAIGLFALVYFWERRSIFISLFALNILVWALAWII
                     STGILEYNEILRLLAIAVAMVLFLALLSGPFVLLFTLYLNGFQILKREGVRFHNFLSM
                     GLAVALTFYLFIAPFVVQSLSAISFFNMVFVYVGFFVSYAIIISMLYTMSSFVNLVNF
                     FPGKLDYVVVLGAGLIGDKVTPLLASRIEKGIAIYQKQPGSKLIMSGGQGPDELIAEG
                     QAMANYALEKGVPAEDILIENQSTNTEENLKFSYALMKPGSRFALVTNYYHVFRALLL
                     ARQLRIKCIGYGAKTKFYFSLNAFIREFVGYVVMSRKAHLVFLSIVSALYLIGVIISL
                     MH"
     gene            complement(14094..14744)
                     /gene="trhA"
                     /locus_tag="AC1PIW_000761"
     CDS             complement(14094..14744)
                     /gene="trhA"
                     /locus_tag="AC1PIW_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAQR family membrane homeostasis protein TrhA"
                     /protein_id="HIP6605717.1"
                     /translation="MNPSLKLSLQLSFGEEVANSVTHAVGALLMLVLLPVTAIHSYQD
                     YNLTAVIGMSIFVISLFLMFLSSSIYHSMPYASTHKYILRIIDHSMIYIAIAGSYTPV
                     ALSLVGGWLGYLIVGLQWGLTIFGILYKIFAKKINEKFSLFLYLFMGWLVVFILPAIL
                     QKTGMAFGILMLAGGLSYTIGALFYAKKKPYFHMIWHLFILLASALQYIAIVYFML"
     gene            complement(14734..15285)
                     /locus_tag="AC1PIW_000762"
     CDS             complement(14734..15285)
                     /locus_tag="AC1PIW_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HIP6605718.1"
                     /translation="MKQTIPYWHELPDLDLYLDQVLLYVNQVVNSQESLEQKSLYSMK
                     INSSLGNEADDRTGVHQGKLITDNVDFRRVLTAAMINNYVKHKQLEKPVKKKYQKQQV
                     ARLIALTILKNVFSIQEISQTLNLLLQSSHSETLYNHFVDCMRNEENEETPDIIRYAC
                     QSVKLYYKTRQLTMELERSHHES"
     gene            complement(15393..17051)
                     /locus_tag="AC1PIW_000763"
     CDS             complement(15393..17051)
                     /locus_tag="AC1PIW_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIP6605719.1"
                     /translation="MKIIIVGGVAGGMSAATRLRRLMENAEIIVFEKGPYVSFANCGL
                     PYYISGEIAEHDSLLLQTPESLKQRFNLDVRPFHEVISIDAEQQTVTVKTPTGELIES
                     YDKLILSPGAKPVIPNLSGIEEANNLFTLRNVPDLDKIMIKLDQIKSGHVVVIGAGFI
                     GLEMAENLALKGWKVMLVEQAPHVLPTFDLEMARYVEAELTNKGVEVITGQSVTAFHE
                     KGQVLELSNGQTLSSDVTILSVGVAPDSQLAKDAGVELGLRGGILVDQNYQTSQKNIY
                     AVGDAIVVKQEITGQDALISLASPANRQGRQVADVIAGLDRKNKGSIGTAIVKVFDLA
                     LAAASTGLSERAVQSLGYKHAVVHTTGKDHAGYYPGATDIVLKLIFSPTDGKIFGAQA
                     VGQKGIDKRIDVLATAIKAGLTIFDLPELELTYAPPFGSAKDPVNMIGYAAMNIAEGI
                     SQNIQWYQLDDELATGKVLLDVRNPGEVANGQFREFIHIPLDQLRERIGELDASKEYI
                     VSCHSGLRSYIAERILKENGFIVKNLDGAYSLYKMTLPKNIIQP"
     gene            complement(17048..17371)
                     /locus_tag="AC1PIW_000764"
     CDS             complement(17048..17371)
                     /locus_tag="AC1PIW_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIP6605720.1"
                     /translation="MFKQLFSGMFSNSDSLPITDVEQAIQSSNAILLDVREVDEFASG
                     HIPQAINQPLSQLDQFKGDKTKNYLVICQSGMRSQRATEYLKSQGYQAINVKGGMNAW
                     TGGIA"
     gene            17478..17738
                     /locus_tag="AC1PIW_000765"
     CDS             17478..17738
                     /locus_tag="AC1PIW_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HIP6605721.1"
                     /translation="MNTDKKKMINRLKRAEGQLRGIQKMIEEDQECIDIVTQLSAVRS
                     SINSMMGMVIAQKVQDCITHPSENPEEQEARLAQAINLIIKK"
     gene            17749..18306
                     /locus_tag="AC1PIW_000766"
     CDS             17749..18306
                     /locus_tag="AC1PIW_000766"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HIP6605722.1"
                     /translation="MSTEKEKMIAGQFYMAADAELRKMRTFARQQMKKFNEELDGKAR
                     SERLKSWFGTTGQRIYMEPNFYCDYDSNIHVGEDFYANFNCTMLDVCPITIGKNAMLG
                     PNVQLLTPLHPLDPDERNSGLEYGSPITIGDNFWAGGGVTILPGVTLGNNVVGGAGSV
                     VTKSFGDNVVLAGNPARVIKEIPKK"
     gene            18365..>18429
                     /locus_tag="AC1PIW_000767"
     CDS             18365..>18429
                     /locus_tag="AC1PIW_000767"
                     /inference="COORDINATES: protein motif:HMM:NF037300.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake Rossmann fold nucleotide-binding
                     protein"
                     /protein_id="HIP6605723.1"
                     /translation="MNNFELFKWKKAGLTNLNVNKV"
BASE COUNT         5381 a         3552 c         4247 g         5249 t
ORIGIN      
        1 tatataaagt ggtatttatt tttggtcgaa ttttacctct tcaagtaggt aattgatgaa
       61 tttttctccc atgtcggaca gcaatgcctt ttcgtgttga atatagacca attcgatttc
      121 atcatcaaat tccagaggaa ttgatacaat attatctccg ttaaggtttg aattgaggat
      181 acctgttgcg attgtatagc catctagacc gattaagaga ttgaagagtg tggcacggtc
      241 agatacgaca atcgattttt tatggtgttc ctgggctaga atttcttctg caaagtagaa
      301 ggagttgtga ataccttgct catagctgag ataggggaaa tctaccaaat catcgagagt
      361 gattgacttt ctttgtgcca aaggattggt tttgctgaca aatacatgtg gtttggcagt
      421 gaagagagtt gtataggata gtcgattgtc atccaacagt ttcagcagaa catcacggtt
      481 gaaatggttg aggaaaagca ctccgatttc tgagcgaaaa ttcttaacgt catcaatgat
      541 ttcccaggtc cgtgtttcac gtaggaagag ttcataatct tccatttcag tttctttcaa
      601 aagtgagaca aaggcgttga cgacaaaggc atagtgttga gaagagacgc taaatagttg
      661 gcgttttgca ttaggatttt tatagcgatc ttctaaaagc tctgtctgtt caacaatttg
      721 acgagcgtag gaaagaaact ccatgccatc ctttgtcagg gtaatgccct tagaattacg
      781 gaaaaagatt ttaattttca tttctttttc caggtctcgt acagcgttgg aaaggctggg
      841 ttgggtaatg aagagttgtt tggcagcctc attcatactg cctgtttctg caattttgat
      901 aatatagtat aattgttgga ttctcatacc tccattgtaa catgatttga caaattattc
      961 taaaaaatgt ataatgctga aaatatatcc tttaactgag tccagagagg cgaagaaggg
     1021 aacgtgataa atagggcaaa tactgccttc ttttcgtgta accttgctag ccagcaaggt
     1081 tttttctcta tatcgacttt cttgttttct gtgtcattgt tatatagaat aggagaaaag
     1141 atgattttaa aagaacagat gttactggtg gaccaagtcc aactagcgcc acgaattttt
     1201 gctatgacct tgcagggtga tatggtccaa gatatgaaaa tggggcagtt tatccatatt
     1261 cgtgtcccag atgattccat gctcttgcgg agaccaatat ctatttccga gattgatcgg
     1321 aataagtctc aatgtcggat tatttaccga gtggaaggtc aagggacgga agtcttttcg
     1381 aaaatgacgg tcgggcagta tttggatgtt atgggaccgc ttgggaaaga gtttgaagta
     1441 gactttctca aagaaggtga ccgcattctc atcattggcg gaggaatcgg tgttcctccc
     1501 ttggtggaag tagccaaaca ggctgcgttc cgtggtgtca atgtcacgtc agtcattggc
     1561 tttgccacca aagaggccgt cattttggaa gaggagctgg caagttatgg gatggtctat
     1621 gtcacaacgg atgatggttc ctatgggcgt aaggggaatg ttgcaacggt cgttgaagag
     1681 ctgacaaacg agtttgcagc catttattcc tgtggagcac ctgctatgat gaactatgtt
     1741 gaccaacgct ttcaggaaca tcctcatgcc tacatttcac tggaggctag gatggcttgc
     1801 gggatggggg cgtgctatgc ctgtgttgtc aaacccaaag agggacaaga acacgagaac
     1861 aagcgtgtct gcaaggaagg tccagtcttt gcgacaggta gtctaatttt gtaggaggtt
     1921 gaaatgacaa aacgattagc gatttcctta ccaggcttgg acttgaaaaa tcccatcatt
     1981 ccagcctctg gctgcttcgg ttttggtcag gaatatgcag attattatga ccttaaccag
     2041 cttggatcta ttatgattaa ggcgactacc cgtcatcctc gttatggcaa tgcgactccc
     2101 cgtgtagccg agacaccagc aggtatgctc aatgccattg gacttcaaaa tccgggtgtt
     2161 gaggcggtct tatccgaaaa attaccatgg ttagagcaac atttcccaga tttgcccatc
     2221 attgccaatg tggctggttt ttccaatgag gaatatgcct atgtgtcagg gaaaatttca
     2281 aaggctccaa acgtaaaagc tatcgagctg aacatttcct gtcccaatgt ggaccacgga
     2341 aataatggtc tcttgattgg acaggtgcca gaattagcct atcaagcagt aaaagcagca
     2401 gtcgaagcct catctgtacc tgtttacgtt aagctgacac caagtgtggc agacataacc
     2461 ctgttggcca aggctgctga ggctgctgga gcgactggct tgacgatgat aaataccttg
     2521 gttggtatgc gattcaatct caaaactcga caaccaatct tggcaaatgg tacaggagga
     2581 atgtcaggtc cagcagtttt tccggttgct ctcaaactca ttcgtcaggt tgctcagatg
     2641 actgatttac cgattattgg tatgggtggt gtggatacgg cggacaaggc tatagagatg
     2701 atggttgcag gagcttcagc cattggggta gggacagcca actttacaga cccatttgct
     2761 tgtccaaaga ttattcaaga cttgccaaaa cgcatggaag tttatggtat tgatagtttg
     2821 gaaggtttac gccgagaaat cagatatagc ttgggtaagc gttgacaaag gtaaaataaa
     2881 agagtaatat aaagaaaata tctcctttaa ctaagtccag agaggctagg aaggtgtcat
     2941 gtaatatgct tgaaaggtga ttttcaagct gaattcgtgc acatccctga ccgatttggc
     3001 agggattttt gtgtaaatga aaggatttta tgaaagaaac aagaccaatt attgcccttg
     3061 actttgcagg taaagaagaa gttcagtctt ttctggaaca gttcccacca gatgagaaac
     3121 tctatgtaaa ggttggaatg gagttgtatt atgcggaggg accaggtatt atcaattatt
     3181 tgaaagactg cggtcacagt atttttctag atttgaaact gcatgatatt cctaataccg
     3241 tcgagtctgc tatgaaagtc ttggcaaaac tcggtgtgga tatgaccaac gttcacgctg
     3301 ctggtggtgt tgagatgatg cgggcagcac gaaggggatt gggtaaggat gctgttttaa
     3361 tagcggtcac tcagttgacc tctacctcag aagagcagat gaggacggac cagaatatac
     3421 agactagttt gcaggaagca gttgttcatt atgctcaaag agcacaagaa gctggtcttg
     3481 atggggtggt ttgttcagct catgaggtgg ctttgattaa agatgctaca agttctgatt
     3541 ttgtgtgcct gacgccaggg attcgtccga ctggcggtga agtaggcgat cagaaacgag
     3601 ttatgacacc agcagatgct gctcgaatcg gtagtgatta cattgtggta ggtcgcccga
     3661 ttacaaaatc agaacatcct taccagacct atttgtctat taaagaggag tggaatcgtg
     3721 gataagattt atcaggttat aatcgataga cctctcgggt atattgataa atttggcaac
     3781 cagtatccaa tcaattacgg ctatgttcca aatctattgg ctggagatgg tgaagagcaa
     3841 gatgtttaca tcatttccaa agctgttcgg cagcccttga cagctttcga ggggaaattg
     3901 gtcgcgatta tacatcgcag agatgatatc gaggataaat gggttctgac aggtcttgat
     3961 gaaaaactta ccgtgacaga aataaaagag aaaaccaatt ttattgacca gtattttgat
     4021 tcatggattg aaatggtgga ctaggagaag tctgtaagaa ttcaacaggg ataaatatag
     4081 ttttttgtag ggttcataga ttgaaccagt actttaggag tgacaggcta ttcggcggga
     4141 tggtcatgtt ggaatggtac gcatttagaa ggaggtctat tatgacacta gcaacagaaa
     4201 tcgcatcaca cttattggat attaaggcag ttcatctgcg tctagaaaag ccatttacat
     4261 gggcatcagg tatcaaatcc cctatctata cagataatcg tattacacta tcttatcctg
     4321 aaactcgaaa tttgattgaa gatggctttg tccaacgtat taaagaagaa ttttctgagg
     4381 ttgaggtgat tgccggaact gccacagcag gtattcctca tggtgctatc attgcagatc
     4441 ggatgaattt gccatttgct tatatccgta gcaaaccaaa agatcatggt gcagggaatc
     4501 aattagaggg tcgcatcgtc aagggacaaa aaatggttgt cgttgaagac ctgatttcta
     4561 ctggagggtc tgttctagat gccgtagcag cagctgagcg tgaaggcgct gacgttattg
     4621 gtgttgtggc aatctttacc tatgaattgc caaaggcaga tcgtaatttt gataatgctg
     4681 gcgtgaaatt ggtgacgctt agcaattatt cagagttaat caaggtcgca aaagtacagg
     4741 gatatatcaa tgcagatggt ttgaatctgc ttaaaaagtt ccgtcaaaat caagaaactt
     4801 ggcaggaaga ctagatttaa aacgagccca cgcacgattt ctgggaactt atcctgtaaa
     4861 atggtataat agtctctaac gtattttgag aatacaataa ttgaccgtat gaggagaaga
     4921 acatgatttc agttatcgtg ccttgtttta atgaagaaga ggcaattccc tatttttacg
     4981 atgctatgga gaaagttcgt aaggagatgg gggaacaatt tgagtatatt tttgtcaatg
     5041 atggttcaaa ggatgggaca ttaacggttt tacgccaatt gtctggtcaa gaccgagctg
     5101 ttcgctatct ttccttttca aggaatttcg ggaaagaagc tgctctctat gcaggtctac
     5161 aagctgcgca aggtgagcta gtgacagtca tggatgttga tttacaagat ccacctgaaa
     5221 tgttgatgga aatgaaggct atgttggatg gaaatccaga tttagattgc gttggaaccc
     5281 gtcgtgtcag tcgtgatggt gaaccaccga ttcgtagctt ttttgcaaaa ttattctaca
     5341 aattgatgaa taaaatcagc caggtcgagg tagtagacgg tgcccgtgat ttccgtctca
     5401 tgcgtcgtca catggtagat gccattctat ctgtgtctga atacaatcgc ttttcaaaag
     5461 gaatttttgc ttgggtagga ttcgaaacag aatacttgcc ctatgaaaat gttgagaggg
     5521 tcgctggcga aactagttgg tctttctgga aattgctttc ttattctatt gaaggaatca
     5581 tcaatttttc agatacacct ttgaatattg cctcctatac aggttttttc accttcctat
     5641 tgtcgctagt tctaatggtt tttgtggttt tcaaaacctt ggtgttcgga gatcctacaa
     5701 ttggctggcc gtcgactatt tgtatcatcc tatttttggg aggtctgcag ttgatgacca
     5761 ttggtattct cggtaaatat ttggcaaaag ttttcttgga aacgaagaaa cgtccgattt
     5821 atattgttaa agagaaaagt tcaaactagg agcagtttat ggaacattca gcttggcacg
     5881 aattgataaa ggcacagttg ccagagcatt attttggtaa aattaatcag tttttagatc
     5941 atgtttatgc tggtggaacc atttatccgc ctcgtgagaa ggtatttgct gccattcaaa
     6001 caacggattt ggaagaggtt aaagtagtta ttttgggaca ggatccctat cacggacctg
     6061 gtcaggcaca gggcttgtca ttttctgttc cgaactcggt accagctccg ccatctcttc
     6121 aaaacatttt aaaagaattg acggatgata ttggcgtcaa acaagatcat gatttgacag
     6181 catgggcgga acaaggtgtg cttttgctca atgcttgttt aactgttcca gcaggtcagg
     6241 caaatggaca tgcagggcaa atctgggagc cttttacgga cgctgttatt aaggtggtca
     6301 atagcttaga ccagcctgtt gtctatattc tttggggtgc ttatgctcgc aagaaaaagg
     6361 cgttgataac caatccaaaa catctggtta tcgaatctgc tcatccaagc cctctttcag
     6421 cctatcgagg tttcttcgga agcaagccct tctcacaagc aaatgcctac ttgacttcac
     6481 aaggcagaac aggaattgat tggctgcgat agtagatggt aatggctttg gtactacaaa
     6541 ggagaatatg aatgttatta gtgaaaaatg gtcgagtatt ggatccgcaa tccggtttag
     6601 acaaggtttg cgacatcttg atggacggaa gtaagattgt taagattgcg gagtcgattt
     6661 cagaagatgt tgagcaagaa gtgattgatg cgacaggatt agtcgttgct cctggtttgg
     6721 ttgatgttca tgtgcatttt cgggagccag gtcagaccca taaagaagat attcatacag
     6781 gagctctttc tgcagcagcg ggtggtttta cttctgttgt catgatggct aacactagtc
     6841 cgacgatttc aaatattgaa accttaaaag aagtattgga ttctgctaaa aaagaagcca
     6901 tccatattta tagtgtggca accattacag atcatttcga tgggcaaaat ctgaccgatt
     6961 ttcctgcttt gctagaagct ggagcggtgg ccttttcgga cgatggtatt cctttgacaa
     7021 atgctggcgt ggttagaaaa gctatgaaat tagccaaaga gcagggctgt ctcatcagtc
     7081 tgcatgaaga agaccctgac ctgaatggtg tcttgggact caacgagcaa gtagctgaaa
     7141 aacattttca tgtttgtgga gcaacaggcg tagcagaata cagtatggtc gctcgcgatg
     7201 tcatgattgc ctatgaaacg ggtgcgcgtg ttcatatcca gcatctatcc aaagccgagt
     7261 cggtcaaggt ggttgaattt gttcaaggtc tcggagcaaa cgtcacggca gaagtagcac
     7321 cccaacattt ttcacgaaca gaagaattac ttctaacaaa aggtgccaat gctaaaatga
     7381 atccgccact acgattagaa agtgatcgct tggctgtgat tgatgggttg aaatctggtg
     7441 ttatctctgt cattgcgacg gaccatgcac ctcatcaccc agatgagaaa aatgtagagg
     7501 accttacgca agctccttca ggaatgacag gcttggaaac ttcgctttca cttggtttga
     7561 cgcatctggt ggaagaaggg catctgacac tcgtacaatt gttggagaaa atgaccatta
     7621 atccagccaa gctctatggt cttgatgctg gctatcttgc tgaaaatggc ccagccgatc
     7681 ttgttatttt tgacccaaat gcaaatcggt tggtcacagc ggattttgcc tcgaagtctg
     7741 caaattctcc gtttgttggt gatagtctga aaggacaggt cctttacacc attgcaaatg
     7801 gacaaatagt atttggtaaa taggaaatga gcggattaga tagctaattc gcttatttcg
     7861 aaccttttta acatgtaagc gattttaatg tttttgtttt ttgataaaaa gtgtataata
     7921 gagatataaa aaatgccgaa gaaaggtcta tttatgaaaa agaaaaaaat cttgttgcct
     7981 gtaatgtcca cactcttgtt ggcgccattt gttttagctc aacaagtaca agctgctgag
     8041 acaacgactg cagctaccac aaccaatcag ccagcaacga ctgatgcaac tgctacggta
     8101 ccagcaacga ctgatgcaac tgctacggta ccagcaacgt cagttgaaaa cgtagcaacg
     8161 gaggaaactg ttgtccctgc tgcggaagaa acagtagagg cagtcattat ccacacaaac
     8221 gacgttcacg gtcgcattct tgaagaaaag aatgtcatcg gtgacgctaa ggctgcggca
     8281 gtcattgaag aagaacgtgc caaagtagaa aatacaattg tagtagatgc tggtgatgcc
     8341 ttccaaggac ttccaatttc caactctaca aaaggtgaag accgcgccaa tatcatgaac
     8401 caagtcggct atgatgcaat ggctgttggt aaccatgaat ttgactttgg gatggatcaa
     8461 gcaatcaagt acaaggaaac cttgaacttc ccgctattga gtgctaacac ttacgttaat
     8521 ggtgctcgtg tttttgaagc ttcaactatt gtcgataaaa ctccaactgt tgtaggcgat
     8581 gaatttgtag ttatcggtgt aacaacacct gaaactgcta caaaaaccca tccgaaaaat
     8641 gttgagggag taacatttac cgacccagtc acagaagtca ataaagtgat tgacgaagtg
     8701 gaagcgcgcg ctctagcaga taatcgtgtt tacaagaact atatcattct agctcaccta
     8761 ggtgtcgatt ctacaacgcc tgttgaatgg agaggttcaa ctcttgctga agcactttct
     8821 aaaaatagta aattagctgg taaacgtgtt attgttatcg acggtcattc ccacacggta
     8881 gaagcgacta cctatggaga taacgttact tataatcaaa caggctctta cttgaacaat
     8941 atcggtaaag taaccttgaa atccgataaa ttattgggtg aagctagctt gatttctgct
     9001 gcggatacta aaaatgtgac gccaaatgct aaaatcgcag ccttggttga tgaaattaaa
     9061 gcaaaatatg aagctgaaaa tgctcaagta gttattgaaa ataacccagt ggaattaaac
     9121 ggagaccgct caaacgtgcg tgttcgcgaa accaacttgg ggaatgcagt gacggatgcg
     9181 atctatgctt atggtcaaac aggattttca aacaaaacta gccttgctgt gactaacggt
     9241 ggtggtctac gtgcaacaat tgctaaagat cagcctgtta caaaaggtga tattattgca
     9301 gtcttgccat ttggtaacat tgtttctcaa atcactgtga ctggtcagca aatttatgat
     9361 atgtttacca agtctttatc atctacattg caagtgaatc cagaaactgg cgagatgctt
     9421 ttagatgaaa atggcatgcc gctctttgaa gctagtggtg gattcctaca catttcagga
     9481 gcaaatgtat tctacgatcc aactcttcct gtagaagaac gtgttcttct tatcggtatt
     9541 cttaatccag agactggcga atatgacgcc ttggatcttg aaaaaacgta ctatttagca
     9601 acaaatgact tccttgctgc tggtggtgat ggctacacaa tgcttggcgg agcacgtgaa
     9661 gaaggtcctt caatggacag cgtctttgct gaatacctaa agacagctga cttatcagct
     9721 tatgaggttg tcaacccata ttcacgtatc attcctgtaa attcaagcat tgatacggat
     9781 gaagatggct acccagactt tatcgaaatt ttattggata ctgatccaga aaatccagcc
     9841 tcaaatccag aaactgttcc agccgaaaat acggattcac catctaatca agtacaaaat
     9901 acttcagcaa cggacaagaa ggccccagtt gattctccga aagtaggaga taaaaaaaca
     9961 gaagtagcaa gtccagcaaa aactacaaaa gcaggagtct tgccaaatac tggtgatcag
    10021 atgaatctga cgctcagcct gtttggttta ggtcttgcag gactggccgt ggcagtaggt
    10081 cgtagaaaag aaaattaatc aatcatctga aaacagggtc ttcggactct gtttttgact
    10141 ggaggaaaca tgcaaactgt atttcaatat caaaaagagg gggattgtga gcagattagt
    10201 tcttacgttc tgcttagcaa ctccccacgt cgtaaacaat tattgggatt tttacaacca
    10261 caaatcgcat cagttgaagt ggatgagcgt ggtatcgaag aacattttat gaaacaattc
    10321 gctacagatg attttctcac tcgagctgca aaaacctgtt gtgaaatttc caaagccaaa
    10381 tcggacatgg atttggtaac tggtcatctc tacatttctg cggatacaat cgtagtgtca
    10441 gatgggcaaa tttttaacaa gcctcaagat ttgacagaag ctgaaaccat gttacgttcc
    10501 tattttggaa agagtcatca cgttgttaca tctgtctgcc tacggacaag acactcactg
    10561 gatgtttttt atacggttgc acagattgat tttgtcgact attatccagc ccttgaagag
    10621 gttattcaat catatatccg tgaaaagcag ccactcgaca aggcaggtgc ctatggtatt
    10681 caggagctgg atccacgctt tgtagcaggt atttctgggg atattcacac cattattggt
    10741 ctacctgtag cagaagtttc ctgcaggatt ttttctgaag aaggttttga aaattagcag
    10801 agcttaacca ttcgtttgtg gtataatagt ggacgaaaag gagagattat gaccgtcgac
    10861 attgaaagat acaagcaatt ggcccagcag aagcaaggag aacatcgaaa atttctagca
    10921 aatttgaaga agaaaacacc taaaaatttg gatagaattg ttcaagatat tcatgccgaa
    10981 gtattttctg aaatcgactg tacagagtgt gccaactgtt gtaaaacatt gggaccactc
    11041 tttacagaag cagatattac ccgaattgcc aagcatttgc gcatgaaact tccagttttt
    11101 gaggatatgt atttgcgtat ggatgaggac ggagataagg tatttcaatc catgccctgt
    11161 cccttccttg gaggcgataa tctatgtagt atctatgatg ctcgtcccaa agcctgtcga
    11221 gaatttccac atactgaccg caagaagatt tatcaaatca acaatttaac aataaaaaat
    11281 actctgattt gtcccgcagc ctatctcttt gtcgaaaagt tacgggagag aatttaagag
    11341 gaacaagtaa gacaaaatat tgattttctg ctataataat aaaaaaacaa gccgttttta
    11401 cggctttttt caatgaatca tatttaggag gaccccatgg caattattca atggtttccg
    11461 ggccacatgt caaaagctcg caggcaggtg caggagaaca tcaagcacgt tgatttcgtg
    11521 accattttgg tcgatgcccg tctgccccta tccagccaaa accccatgtt gaccaagatt
    11581 gtcggagaca agcccaagct catgattctc aacaaggtcg acttagcaga ccctgttcgt
    11641 accaaagaat ggcagagcta ctttgaaggt caaggcatcc gtacccttgc catcaactca
    11701 aaagagcaag cgacggtgaa aaaggtgacc gacgcagcca agggtctgat gaaagacaag
    11761 attgaacgcc tccgcgagag aggtattcaa aaagaaacct tgcggaccat gatcatcggt
    11821 attccaaacg ctggaaaatc caccctcatg aaccgtctgg ctggtaagaa aatcgccgtt
    11881 gttggcaaca aaccaggtgt gacgaagggc caacagtggc tcaaatccaa caaggacctt
    11941 gaaatccttg atacacctgg gatactctgg cctaaatttg aggaccagga agttggactt
    12001 aagttggctc tgacaggagc cattaaggat cagctcttgc ctatggatga agtgactatt
    12061 tttggtttgg actacttcaa agccaactac ccagaacgtc tacaagagcg atttaagggg
    12121 atggatttgg agcaggaaac acctgaactc atcatggact ggaccaagaa attgggcttt
    12181 cgtgatgatt atgaccgttt ttacaacctc tttgtcaagg acgttcggga cggtcgcttg
    12241 ggtacttata cattagacag ggtgagtgac ttagatggca acgattaagg aagtgacagc
    12301 tctgctggcc caggtagaca gtctggacag tccagtctgg gaccaactgg ctcaggatga
    12361 gcgagcaggg gtccaggctg ctatcaagaa acgccgtaag gagctggaaa aagaagcggt
    12421 agaagatgcc cgcttagaag ctatgctatt ctacgaaaaa tccctttatg aaaatggggt
    12481 ggagttcatc gctggtatcg acgaagtcgg ccggggacct ctagcaggtc ctgtggtggc
    12541 agcggcggtt atcttgccta aagggtgcaa aatccgctac ctcaacgact ccaagaaaat
    12601 ccccaagtcc aagcatgaag caatctacca agaggttatg gagcgagcgg tggcagtcgg
    12661 tgtgggaata aaagatgcag ctctcatcga cgaggtcaac atctatgagg caaccaagtt
    12721 agccatgctg gaggctttgg ggaaattaag ccagaaacct gaccacttgc tcatagacgc
    12781 catgaagttg gatacgccca taccacaaac ttccatcatc aagggagatg ccaatagcct
    12841 atctatcgca gcggcctcta ttgtagccaa ggtgacgcgg gataagatga tggcggacta
    12901 tgacaaggag ttctcaggct acggcttcgc caagaatgct ggctatggaa cgacagagca
    12961 cttggaaggc ttgaacaagt taggcatcac gcccattcac cgtaagacct ttgagcccat
    13021 taagtctatg gtagcagggg gaaactagtc ttgacagtct ttttctggtt atttttagcg
    13081 gctatcggtc tgtttgctct tgtctatttc tgggagcggc ggtcgatttt tatctcactg
    13141 tttgctttga acatcctcgt ctgggcttta gcatggatta tctcaacagg cattctcgaa
    13201 tacaatgaaa tcttgcgctt gctggctata gcagtggcta tggtgctctt tttagccttg
    13261 ttaagtggtc catttgtctt gcttttcaca ctttatctaa atggttttca aattttgaag
    13321 agagaagggg ttcgcttcca taatttccta tccatgggac tggcggtagc cttgactttc
    13381 tatctcttca tcgctccctt tgtggttcaa tctctatcag ccatcagctt tttcaatatg
    13441 gtgtttgtgt acgttggttt ttttgtgtcc tatgccatta tcatcagcat gctctatacc
    13501 atgtctagct ttgtcaattt ggtcaatttt ttcccaggca agcttgatta tgtagtggtc
    13561 ttgggagcag gtctgattgg tgacaaggta acgccacttt tggcttcgcg gattgaaaaa
    13621 ggcatagcga tctaccagaa acagcctggc agcaagctca tcatgtcagg cggacaaggt
    13681 cctgatgaac tcatagcaga aggacaggca atggcaaatt atgccttgga gaaaggagtg
    13741 cctgctgaag acattctcat cgaaaaccaa tcaaccaaca cagaagaaaa tttgaaattt
    13801 tcctatgccc tgatgaaacc tggcagtcga tttgcacttg taaccaatta ctatcacgtt
    13861 ttcagagctc tgctattggc acgacaattg agaatcaaat gtatcggcta cggcgcaaaa
    13921 accaaatttt actttagcct taatgccttc attcgggaat ttgtaggcta tgtagtcatg
    13981 agccgaaagg cgcatttggt ctttttgagc atcgtgtcag ccctttactt gattggagtg
    14041 atcatcagtt taatgcatta aaaacagcct ctaggtgact agaagctgtt ttcttataac
    14101 ataaagtaga caatggcgat atactgaagt gccgaagcca gtaggataaa gagatgccaa
    14161 atcatgtgga agtagggctt tttcttggca tagaagaggg ctccaatagt ataagataat
    14221 ccaccagcca gcatgagaat gccaaatgcc attccagtct tttgcagaat ggctggcagg
    14281 ataaagacca ccaaccagcc cataaagaga tagaggaaaa ggctgaattt ttcattgatt
    14341 tttttggcaa atattttgta gagaatacca aaaatagtta atccccattg taaacctaca
    14401 atcagataac ctagccaacc accgaccaag gaaagggcaa caggtgtgta gcttccagcg
    14461 atggcaatgt aaatcatgct atggtcaata atcctcagga tgtatttgtg agtagaagca
    14521 tagggcatgg agtggtagat ggaagaggat aggaacatga gaaataggct gatgacaaag
    14581 atggacatac caatcacagc ggtcaggtta taatcttgat aggaatgaat agcagtaacg
    14641 gggagcaaga ccaacataag cagggctccg actgcatgtg tgacactgtt agcaacctcc
    14701 tctccaaagg atagttgcag gctcaatttc aaactaggat tcatggtgac tcctttctaa
    14761 ctccatagtg agttgacggg ttttgtagta aagtttgaca gattgacaag cgtagcgaat
    14821 gatgtcaggg gtttcttcat tttcctcgtt tcgcatgcag tcaacaaaat gattgtagag
    14881 tgtttctgaa tgactcgatt ggagaagaag atttaaggtc tgactgattt cttgaatcga
    14941 aaagacattt tttaagatag tcaaggcaat caagcgggca acttgctgtt tctggtattt
    15001 tttctttacc ggtttttcaa gttgcttgtg cttgacataa ttgttaatca tagcagcagt
    15061 caatactctt cgaaaatcaa cattatccgt tatcaacttg ccttgatgaa ccccagttct
    15121 atcatcagct tcgtttccta ggctactgtt gattttcatt gagtataaac tcttctgttc
    15181 aaggctttcc tgagaattga ctacttgatt aacatacagc agaacttggt ccaaatacaa
    15241 atctaaatca ggtaattcat gccagtaggg aatggtctgt ttcatgatta tctactctct
    15301 tttatctagt aatgataact agattgtacg atatacaata tttaatgtca agaaaaaacc
    15361 ttctggaccc aaaatcacag aaggttagtt gcttatggtt ggataatatt tttaggcaat
    15421 gtcatcttat aaagtgaata ggctccatct aggtttttaa ctataaaacc attttctttt
    15481 aagatacgtt cggcaatata agaacgcagt ccactatggc agctgacaat gtattctttt
    15541 gaagcatcca gttctccaat tcgctcacgg agttggtcca aaggaatgtg aatgaattct
    15601 ctgaattgcc catttgcgac ctcaccagga ttgcggacat ctaatagaac tttaccagta
    15661 gctaactcgt cgtctagttg ataccactgg atgttttggc taatcccttc tgcgatgttc
    15721 atagcagcgt agccaatcat attgacaggg tctttggcag agccaaatgg cggagcgtaa
    15781 gtcaattcta gctctggtaa atcaaaaatt gtcagtccag ccttgatagc agtggcaagg
    15841 acatcaatcc gcttatcaat acctttttga ccgacggctt gcgctccgaa aatttttcca
    15901 tcagtcggtg agaaaatgag ttttaaaaca atatcagtcg caccaggata ataacctgca
    15961 tggtcttttc cagttgtatg aacgacggca tgtttatagc cgagtgattg aacagcacgt
    16021 tcgcttaaac cagttgaagc ggcagctaga gctaggtcaa agacttttac gatagctgtt
    16081 ccaatagatc ctttgttttt tctatcaagt ccagcaatga catcagctac ctgtcttccc
    16141 tgacggttag caggagaagc cagtgaaatc aaggcatctt gacctgtaat ttcttgttta
    16201 acaacaatag catcaccaac agcataaata ttcttctgac tagtttggta gttttggtct
    16261 acgagaatac caccacggag acctaactca actccagcat ctttggctag ttgactgtcg
    16321 ggagcaactc cgactgagag aatggtcaca tcagatgaca aggtttgtcc gttagacaat
    16381 tctagaacct gtcctttttc atgaaaagca gtaacagact gcccagtgat aacctcgaca
    16441 cctttattgg ttagctctgc ttccacatat cgtgccattt ctaagtcaaa agtaggtaaa
    16501 acatggggag cttgttcgac aagcatgacc ttccagcctt ttaaagctaa gttttccgcc
    16561 atttccagac cgataaaacc tgcaccaatt accacaacat gaccagactt gatctggtct
    16621 agtttaatca taatcttgtc caaatcaggt acgttccgaa gagtaaagag attgtttgct
    16681 tcttcaatac cagatagatt tggaatgaca ggcttggcgc cgggtgataa gattagtttg
    16741 tcgtagcttt caatcaactc accagtaggc gttttgactg ttacagtttg ctgctcagca
    16801 tcgatactaa tgacttcatg gaaggggcgg acatctagat taaagcgttg tttcaaactt
    16861 tctggcgttt gaagcaataa actgtcatgc tctgctattt ctccagaaat gtaataaggt
    16921 aaaccgcagt tggcaaagga aacataaggt cctttctcaa aaacgatgat ttcagcattt
    16981 tccatcaaac gacggagacg agtagctgct gacataccgc cagcgacacc acctacaata
    17041 attattttca tgctattcct cctgtccagg cattcatacc gcctttaacg ttgattgctt
    17101 gatagccttg agatttaaga tattctgtcg cccgttggct acgcatgcca gattgacaga
    17161 tgacaagata gttcttggtt ttgtcgcctt taaattggtc aagttgactt aagggctggt
    17221 tgatagcttg tggaatatgt ccactagcaa attcgtcgac ttcgcgaaca tcaagcaaaa
    17281 tagcattcga tgattgtatc gcttgttcta catcagttat cggaagacta tctgagtttg
    17341 aaaacattcc tgaaaataat tgtttaaaca ttgttactcc tttataccct acagggtatc
    17401 taaaatatat caaggaattc gaaagatgtc aaggtaaaaa attgacattt ttcacttcaa
    17461 gcagtaccat atagggtatg aatactgata agaaaaagat gataaaccgt ttgaaacggg
    17521 ccgaaggtca gctcagaggc attcagaaaa tgattgaaga agatcaggaa tgtattgata
    17581 ttgtaactca gttgagtgct gttcgttcca gcattaatag tatgatggga atggttattg
    17641 ctcagaaggt acaagattgt atcactcatc catctgaaaa ccctgaagaa caagaagctc
    17701 gtcttgctca agctatcaat ttaatcataa aaaaatagga gtaggggtat gtcgacagaa
    17761 aaagaaaaga tgatagctgg gcaattttat atggcagcag atgctgaatt gagaaagatg
    17821 cgaacctttg cccgccaaca gatgaaaaaa tttaacgaag aactagatgg aaaagcccgc
    17881 tctgagcgat tgaaaagctg gtttggaact acaggccaac gcatctatat ggagcccaat
    17941 ttctactgtg actacgatag caatattcat gttggtgagg atttttacgc taatttcaac
    18001 tgtaccatgt tggatgtctg tcccattacc attggcaaaa atgccatgct tggtcccaat
    18061 gtacagttgc taactccact tcatccacta gatcctgatg aacgaaattc tggtttggaa
    18121 tatggctctc ctattaccat tggtgataat ttctgggctg gtggtggtgt gacaatctta
    18181 cctggcgtga ccttgggcaa caatgttgtt ggtggtgctg gttccgttgt gaccaagtct
    18241 tttggtgata atgtcgtttt ggctggcaat cctgcgcgtg ttatcaaaga aatacctaaa
    18301 aaataattct tcttcgggag aattttttct ttgccttgcg aatagactag atgaggtgaa
    18361 gaaaatgaat aattttgaat tgtttaaatg gaaaaaagca ggtttgacca atcttaatgt
    18421 gaataaagt
//
LOCUS       DBNFCK010000028        18336 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.028, whole genome shotgun sequence.
ACCESSION   DBNFCK010000028 DBNFCK010000000
VERSION     DBNFCK010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 18336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18336
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.028"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            141..1397
                     /gene="pcsB"
                     /locus_tag="AC1PIW_000768"
     CDS             141..1397
                     /gene="pcsB"
                     /locus_tag="AC1PIW_000768"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan hydrolase PcsB"
                     /protein_id="HIP6605724.1"
                     /translation="MKKKILATIMLSTVVLSNANYVAVISANDVDSQIATKNQQISEL
                     TAQQAEAQQQVDAIQGQVDAIVSEQAKLTEENTRLEAESQTLAADIERLSADIVSRDG
                     ALKEQARSAQVDGSASSYINTILDSKSIIDAVSRVNAMREIISANNRMLEQQKADKEA
                     IVEKQKANQEAITTLAANRQKLEDDAQVLQVRQAELEAAKLNLAVQKATAEDEKNSLL
                     VQKAAAEEAARQAAARQAEYQAQQAALAQQQVASVSAPVVSTLVETTVTETVAAPTQT
                     VSQSTPTVSTPTTSTSSGSGSSAAANNARYDASSYPVGECTWGVKSQLSWVGPYWGDA
                     KQWLASARAEGFSTGSTPQVGAIAVWTGGYYGHVAVVTAVQSSTSIQVVESNYMGRRY
                     IGNHRGGYFNPTTTSEGAVYYIYPPY"
     gene            1500..2468
                     /locus_tag="AC1PIW_000769"
     CDS             1500..2468
                     /locus_tag="AC1PIW_000769"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIP6605725.1"
                     /translation="MAFTDLKLFALSSNQKLAEQVSKKIGIPLGKSSVRQFSDGEIQV
                     NIEESIRGTHVYILQSTSSPVNDNLMEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLQTAGVNRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGDGYVVVSPDHGGVTRARKLAQFLKTPIAIIDKRRSVDKMNTSEVMNIIGDIKDK
                     TCILIDDMIDTAGTICHAADALADAGATAVYASCTHPVLSGPAMDNISKSAIKKLVVL
                     DTIEIAEDRLIDKIEHISTAELLAEAIIRIHEKRPLSPLFEASRVK"
     gene            2555..3733
                     /locus_tag="AC1PIW_000770"
     CDS             2555..3733
                     /locus_tag="AC1PIW_000770"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIP6605726.1"
                     /translation="MDLLNRFNKNLNRIEVSMIRQFDQSISDVPGILKLTLGEPDFTT
                     PDHVKEAAKAAIDADQSYYTGMAGLLELRQAAAEFVAEKYNLHYNPENEILSTIGATE
                     ALSASLVAILEAGDTVLLPAPAYPGYEPIVNMVGADIVEIDTTANDFVLTPEMLEEAI
                     IEQGDKLKAVILNYPANPTGVTYSREQIQAFADVLRNYPVFVLSDEVYAELTYTGQPH
                     TSIAEFLPEQTILIQGLSKSHAMTGWRIGLIMSQAPIIAQIIKSHQYLVTAASTAMQY
                     GAVEALKNGKDDALPMRAEYVKRRDYIIEKMTDLGFKIIKPDGAFYIFAKIPAGYNQD
                     SFSFLQDFARKKAVAFIPGAAFGQYGEGYVRISYAASMEKIQTAMARLKEYLEENGTN
                     "
     gene            3720..4502
                     /gene="recO"
                     /locus_tag="AC1PIW_000771"
     CDS             3720..4502
                     /gene="recO"
                     /locus_tag="AC1PIW_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIP6605727.1"
                     /translation="MERIETRGLVLYNRNFREDDKLVKIFTEKAGKRMFFVKHASKSK
                     LVASIQPLTYADFIVKINDDGLSYIEDFHQVQPFKNINGDIFKLSYATYILALADAAL
                     QDKVYDPALFAFLVKTLDLMESGLDYEVLTNIFEIQLLGRFGISLNFHECAFCHRVGL
                     PFDYSYKYSGVLCPQHYQQDERRAYLDPNVPYLLDQFQAISFDELETISIKPEMKRKL
                     RFFIDQLYEEYVGIHLKSKKFIDDLSSWGQIMKPRTENEETE"
     gene            4499..5506
                     /gene="plsX"
                     /locus_tag="AC1PIW_000772"
     CDS             4499..5506
                     /gene="plsX"
                     /locus_tag="AC1PIW_000772"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HIP6605728.1"
                     /translation="MKRIAVDAMGGDHAPQAVVEGVNQALAAFPDIEIQLYGDEAKIK
                     QYLTATERVSIVHTTEKINSDDEPVKAIRRKKEASMVLATKAVKDGQADAVLSAGNTG
                     ALLAAGVFVVGRIKNIDRPGLMSTLPTMDGKGFDMMDLGANAENIAHHLYQYGILGSF
                     YAEHVRGVKQPRVGLLNNGTEDTKGTPVHQEAYKLLAEDKSINFIGNVEARELLNSVA
                     DVVVTDGFTGNAVLKTIEGTAKSIVGQLTGSIKNGGLRAKLGGLLVKPTLKKALGAMD
                     YKTAGGAVLLGLKAPVIKAHGSSDAQSIFYTIKQTRSILEAGIVEKSVAKFSVVEESH
                     D"
     gene            5499..5747
                     /locus_tag="AC1PIW_000773"
     CDS             5499..5747
                     /locus_tag="AC1PIW_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HIP6605729.1"
                     /translation="MTREQVYQRVVELIQDEKGEDFQVQPESTLADNIAADSVEIMEF
                     VLNLEDEFHVDVPDAAIEHFEVLSDIVDFIYKEVKKRS"
     gene            5865..6572
                     /gene="purC"
                     /locus_tag="AC1PIW_000774"
     CDS             5865..6572
                     /gene="purC"
                     /locus_tag="AC1PIW_000774"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIP6605730.1"
                     /translation="MKTDLLYSGKAKDIYATADSDQIVAVYKDQATAFNGGKKEQIVG
                     KGRLNNLISSLIFEKLNEAGVPTHFIKRLSDSEQLNKKVEIIPLEVVLRNVTAGSFSK
                     RFGVEEGITLSTPIVEFYYKKDELDDPFINDEHIAFLELASQDQIAYIKEETRRINEF
                     LKDLFAQIGLTLVDFKLEFGIDSSGQILLADEFSPDNCRLWDADGNHLDKDVFRRGLG
                     ELTEVYEVVLAKLQEVK"
     gene            6585..10304
                     /locus_tag="AC1PIW_000775"
     CDS             6585..10304
                     /locus_tag="AC1PIW_000775"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HIP6605731.1"
                     /translation="MAKRIFVEKKAAFQIKAEALRKELTHNLQLTSLSSLRLVQVYDV
                     FNLEEDLLEQAIKHIFTEQVTDKVLSDEELGLEGAVYFAIEALPGQFDQRAASSQEAL
                     LLLGSRQEVRVNTGQLYILNGDVQEEELVAIKNYLLNPVDSRFKDMDAPLVAQEFSVS
                     DTVIPSLDFFDSYGVEEFASYKREAGLAMEVEDLLFIQDYFKSIGRVPTETELKVLDT
                     YWSDHCRHTTFETELRSIDFSASKFQKQLQATYDKYLAMRTELGRTDKPQTLMDMATI
                     FGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPFGG
                     AATCIGGAIRDPLSGRSYVYQAMRISGAGDITQPLTATRSGKLPQQIISKTAAHGYSS
                     YGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKENVVREKPVAGDVVILLGGKTG
                     RDGIGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGAVTRLIKKSNDFGA
                     GGVCVAIGELADGLEIDLDKVPLKYAGLNGTEIAISESQERMSVVVRPEDVDAFIAAC
                     RQENIHAVVVAKVTEKPNLVMTWNGQTIVDLERSFLDTNGVRVVVDAKVVDSPVNLPE
                     TRTTSAQTLREDLKDLLSDLNHASQKGLQTIFDSSVGRSTVNHPLGGRHQLTPTESSV
                     QKLPVQQGVTTTASVMAQGYHPYLADWSPYHGAAYAVIEATARLVATGANWSKARFSY
                     QEYFQRMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEDLTVPPTLVAF
                     GVTTADSCKVLSPEFKATGEHIYYLPGQILSEDIDFTLIKSNFKAFEKWQSDYVITAA
                     SAVKYGGVLESLALMSFGNQVGARVELADFETSLTGQLGGFVFTSQEDIPDAVKIGQT
                     TTDFTLVVNGVNLSGQDLQVAFEGKLEEVYPTEFEQATELQDVPAVTSSAVIKAKETV
                     EVPVVYIPVFPGTNSEYDSAKAFEQAGAKVNLVPFVTLDAESIENSVDTMVDNIAKAN
                     ILFFAGGFSAADEPDGSAKFIVTILRNAKVRSAIDQFIEKRGLIIGICNGFQALVKSG
                     LLPYGNFEEAGDTSPTLFYNDANQHIAKMVETRIANVNSPWLAGVQVGDIHAIPVSHG
                     EGKFVVTDEEFATLRDNGQIFSQYVDFTGQPSMDSKYNPNGSSHAIEGITSRNGQIIG
                     KMGHSERYEDGLFQNIPGKKDQGLFVSAVRYFTGK"
     gene            10307..11761
                     /gene="purF"
                     /locus_tag="AC1PIW_000776"
     CDS             10307..11761
                     /gene="purF"
                     /locus_tag="AC1PIW_000776"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009908857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIP6605732.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPQAAQVTYFGLHSLQHRGQEGAGI
                     LANDGGQLCRHRGTGLIAEVFKNPADLEALTGTAAIGHVRYATSGSASINNIQPFLFD
                     FADMQVGLAHNGNLTNAVSLKAELEKNGSIFSSSSDTEILMHLIRRSHNPDFMGKIKE
                     ALNTVKGGFAYLILLEDKLVAALDPNGFRPLSIGKMKNGAWVVASETCAFEVVGADWV
                     RDVEPGEIVVIDDSGIQYDSYTRDTQLAVCSMEYVYFARPDSVIHGVNVHTARKNMGR
                     RLAQEFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSSVVKGKRVVMIDDSIVRGTTSRRIVQLLRDAGAKEVHVAIGSPELKY
                     PCFYGIDIQTRRELISANHTVEEVCEIIGADSLTYLSLEGMIEAIGIETDAPKGGLCV
                     AYFDGEFPTPLYDYEEEYLRSLEEKTSFYIENVK"
     gene            11817..12839
                     /gene="purM"
                     /locus_tag="AC1PIW_000777"
     CDS             11817..12839
                     /gene="purM"
                     /locus_tag="AC1PIW_000777"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIP6605733.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERLGVMGALGGFG
                     GMFDLTKLDVKEPVLVSGTDGVGTKLMLAIQYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYIATGKNEPAKLEQVVAGVAEGCVQAGCGLIGGETAEMPGMYGEDDYDLAGFAV
                     GIAEKSQIIDGSKVQEGDILLGLASSGIHSNGYSLVRRVFADVSGDALLPELNGRALK
                     DVLLEPTRIYVQQVLPLVKAGLVNGIAHITGGGFIENVPRMFADNLAAEIEEDKIPVL
                     PIFTALETYGQIKHEEMFEIFNMGIGMVLAVSPGKVEKVRELVGEEVYEIGRIITKED
                     KSVVIK"
     gene            12836..13387
                     /gene="purN"
                     /locus_tag="AC1PIW_000778"
     CDS             12836..13387
                     /gene="purN"
                     /locus_tag="AC1PIW_000778"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIP6605734.1"
                     /translation="MKRIAVFASGNGSNFQVIAEQFEVAFVFSDRRNAYVLERAEKLG
                     VPTFAFELKEFADKQAYEEAIIQLLDQHQIDLVVLAGYMKIVGPTLLAQYEGRIINIH
                     PAYLPEFPGAHGIEDAWNAGVAESGVTVHWVDSGIDTGQIIKQVRVPRLADDILETFE
                     ARIHEAEYQLYPAVLEELGAVKK"
     gene            13397..14944
                     /gene="purH"
                     /locus_tag="AC1PIW_000779"
     CDS             13397..14944
                     /gene="purH"
                     /locus_tag="AC1PIW_000779"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HIP6605735.1"
                     /translation="MTKRALISVSDKNGIVEFAQELTKLGWEIISTGGTKVALDNAGV
                     ATIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDVDSHLQAAKDHEIGLIDLVVVNL
                     YPFKETILRPDVTYDLAVENIDIGGPSMLRSAAKNHASVTVVVDPADYPTVLGEIAEQ
                     GQTTYPTRQRLAAKVFRHTAAYDALIADYFTKQVGEDKPEKLTITYDLNQPMRYGENP
                     QQNADFYQNALPTDYSIAAAKQLNGKELSFNNIRDADAAIRIIRDFKDRPTVVALKHM
                     NPCGIGQAETIEKAWDYAYEADPVSIFGGIVVLNREVDAATAEKMHPIFLEIIIAPSY
                     SAEALAILTNKKKNLRILELAFDAQDASEVEKEFTGVVGGLLVQDQDVVVESPADWQV
                     VTERQPSEQEWAAMEFAWKSSKYVKSNGIIITNDKMTLGVGPGQTNRVASVRIAIEQA
                     KDRLEGAVLASDAFFPFADNVEEIAAAGIKAIIQPGGSVRDQDSIDMANKYGLTMVFT
                     GVRHFRH"
     gene            15070..16332
                     /gene="purD"
                     /locus_tag="AC1PIW_000780"
     CDS             15070..16332
                     /gene="purD"
                     /locus_tag="AC1PIW_000780"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIP6605736.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESEQVEQVFVAPGNDGMTLDGIEL
                     VNIGISEHSAIINFAKENDVAWTFVGPDDALAAGIVDDFEQAGLKAFGPSRLAAELEW
                     SKDFAKQIMVKYGIPTAAFGTFSNFEEAKAYIEEQGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAREMLLDNKFGDSGARVVIEEFLAGEEFSLFALVNGDQFYILPTAQDHKRAF
                     DGDQGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPILEGMIADGRSYLGVLYAGLILT
                     DQGPKVIEFNARFGDPETQIILPRLTSDFAQNIDDILHKRPTQLTWLDSGVTLGVVVA
                     SNGYPLDYEKGVELPAKTEGDITTYYAGARFAENSRALLSNGGRVYMLVTTADTVQDA
                     QEKIYSELKNQDTTGLFYRTDIGSKAVK"
     gene            16358..16846
                     /gene="purE"
                     /locus_tag="AC1PIW_000781"
     CDS             16358..16846
                     /gene="purE"
                     /locus_tag="AC1PIW_000781"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HIP6605737.1"
                     /translation="MNIPISIIMGSSSDWKTMKKAADMLDKFGVAYEKKVVSAHRTPD
                     LMFRHAEEARGHGFKVIIAGAGGAAHLPGMVAAKTTLPVIGVPVQSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALTALRILSIEDQTIAAQLADFAKEQEKIAEAMTDD
                     LI"
     gene            16833..17912
                     /gene="purK"
                     /locus_tag="AC1PIW_000782"
     CDS             16833..17912
                     /gene="purK"
                     /locus_tag="AC1PIW_000782"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HIP6605738.1"
                     /translation="MTSSKTIGIIGGGQLGQMMAISAIYMGHKVITLDPAADCPASKV
                     SEVIVAPYHDVAALKQLAERCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLAKKAGVQVAPYKVVTSSLDLEGLDLSKNYVLKTATGGYDGHGQKVIREEAD
                     LVEASQLANSAECVLEEFVNFDLEISVLVSGNGSDYTVFPVQENIHRNNILYKTIVPA
                     RIFDELAEKAKTMALQIADKLHLVGTLCVEMFVAGQEILVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHIRGILGEPLPPIRLLSPAVMINVLGQDMETVQTFLQENPTAHPHFYGKLE
                     AKHNRKMGHVTVLGEDAESVEEFEN"
     gene            17956..>18336
                     /locus_tag="AC1PIW_000783"
     CDS             17956..>18336
                     /locus_tag="AC1PIW_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605739.1"
                     /translation="MPLPQKIQEEIKRYCNNHLPNNDWYEKEFDFIHDVSLKNRIIRE
                     FKSIRYAYKLYEGITAEEEHLIFEIRSQILAYASIYEAVVEYVLETYYSDTQVYDDLV
                     HQNNVMTKIDIPEEKRKKLERELIH"
BASE COUNT         5016 a         3746 c         4662 g         4912 t
ORIGIN      
        1 ttttcgtgtt acaaccatta tttgagcgtt tatttggaat aacaaacaag acatagaaat
       61 gtaataattg cgtaatatac ttacgcgatt tttttgataa aatgagagag tcagaaagta
      121 tagaggagtt atgttcggat atgaagaaaa aaatcttggc tacaattatg ttaagtacag
      181 tcgttctatc taatgctaat tatgtagctg tgattagtgc gaatgatgta gatagtcaga
      241 ttgcaacaaa aaatcaacag attagtgagt tgacagcaca acaagcagaa gctcaacaac
      301 aagttgatgc tattcaagga caagttgatg caattgttag tgaacaggcg aaattaacag
      361 aagaaaatac tcgtttggaa gcagaatcgc agacattggc ggcagatatt gagcgtttgt
      421 cagctgatat tgtgtcacgt gatggtgctt taaaggagca ggcgcgtagt gctcaagttg
      481 atggttctgc ttcaagttac attaatacaa ttttggattc aaaatcaatc attgatgctg
      541 tttctcgtgt taatgcaatg cgtgagatta tttcagctaa taaccgtatg ttagaacaac
      601 aaaaggctga taaggaagca attgttgaaa aacaaaaggc aaatcaagag gcaatcacta
      661 ctttggcagc taatcgccaa aaattggaag atgacgccca agtattgcaa gtgcgtcagg
      721 ctgaattgga agctgctaag ttaaatttgg ctgtgcagaa agctactgct gaagatgaga
      781 aaaattcatt gttggtgcaa aaggcagctg cagaagaagc agctcgtcaa gcggcagctc
      841 gtcaagcgga gtatcaagca caacaggcag ctctagcaca gcaacaagta gcctcagtat
      901 cagcaccagt cgtgtcaacg ctagtagaaa ctacagtgac tgaaacggtt gcagccccta
      961 ctcagacagt atcacaatct acaccaacgg taagcacacc tacaacgtct acatcatcag
     1021 gttcaggtag ttcagcggca gcaaataatg cgcgttatga cgcttcatct tacccagttg
     1081 gtgagtgtac ttggggagtt aaatctcaac tttcatgggt tggtccttac tggggagatg
     1141 ctaaacagtg gttagcatca gcacgtgctg aaggttttag tacaggttct actccacaag
     1201 taggtgcgat tgctgtttgg acaggtggtt attatgggca cgtagcggtt gttacggctg
     1261 ttcaatcttc aacaagcatc caagttgttg aatcaaacta catgggtcgt cgttatattg
     1321 gtaaccatcg tggtggttat ttcaatccaa caacgacttc tgagggagcg gtttactaca
     1381 tttaccctcc atattaagaa attttcaaat agagcgtgtg gatttgcgct ctatttttgt
     1441 ttgaaaaatg aaggaaaaag ggttaaaata gtaaaggtag aaatatatag gaggctgtca
     1501 tggcgtttac tgacttaaaa ttgttcgctc tttcgtcaaa tcagaagtta gctgaacagg
     1561 tgtcgaaaaa aataggaatt cctctgggaa aatcaagtgt tcgtcaattt tcagatggtg
     1621 agattcaggt aaatattgag gaatccattc gtggaactca tgtgtatatt cttcaatcca
     1681 ctagctcacc agttaatgat aatttaatgg aaattttgat tatggtcgat gctcttaaac
     1741 gtgcatctgc tgaatcagta aacgtagtaa tgccttatta tggttatgca cgccaagacc
     1801 ggaaggctcg tgcacgggaa ccaattacat caaaattggt ggctaatatg ttgcagacag
     1861 cgggggtcaa tcgtttgttg acaattgatt tgcatgctgc gcagattcag ggattcttcg
     1921 atattcctgt tgatcatttg atgggtgctc cattgattgc ggattatttt gagcgtcgtg
     1981 gtatggtagg tgatggctat gttgttgtat cccctgacca tggtggtgtg acacgtgccc
     2041 gtaagttagc acagttcttg aagacaccga ttgctattat tgataaacgc cgtagtgttg
     2101 ataagatgaa tacatcagaa gtgatgaaca ttattggcga tattaaggat aaaacttgta
     2161 tcttgattga tgatatgatt gatactgcgg gtaccatctg tcatgcagcg gatgctttgg
     2221 cagatgcagg ggcaacagct gtttatgctt catgtacgca cccagtattg tcagggcctg
     2281 ctatggataa cattagtaag tcagcaatta agaaattggt tgttcttgat acaattgaaa
     2341 ttgcagaaga tcgcttaatc gataaaatcg aacatatttc aacggcagaa ttattagcgg
     2401 aagcgattat tcgtattcat gaaaaacgtc ctttgtcacc tttgtttgag gcaagtcgtg
     2461 ttaaataggt atgagaagag tcaaatgact cttctttttt acatttattt tcaaaatgta
     2521 ttataatagt tttatatgtt tttgaggtgg cagaatggat ttattgaatc gttttaataa
     2581 gaatttgaat cggattgagg tttcgatgat tcgtcagttt gatcagtcta tttcggatgt
     2641 tcccgggatt ttgaaactga ccttgggaga gcctgatttt acaacgcctg atcatgtaaa
     2701 agaagcagct aaggctgcta ttgatgctga tcagagttat tatactggaa tggctggtct
     2761 tttggagttg cgtcaggctg cagctgagtt tgtggctgaa aaatataatt tacactacaa
     2821 tccagaaaat gaaatcctct ctacaatcgg tgcgacagaa gctctgtcag cgagtttagt
     2881 tgctattttg gaggctgggg atacagttct tctgcctgcc cctgcctatc ctggttatga
     2941 gccgattgtc aatatggtgg gagcagacat cgttgaaatc gatacgacgg caaatgattt
     3001 tgtattgaca cctgagatgt tggaagaggc gattattgag cagggtgata agctaaaagc
     3061 tgtcattctc aactatcctg ccaatccaac aggtgtgacc tactcacgtg agcagattca
     3121 ggcttttgcg gatgtcttgc gtaactatcc tgtctttgtc ctatcagatg aggtctatgc
     3181 ggagttgact tacactggtc agcctcatac ttcgattgcg gagtttttgc cagaacagac
     3241 cattttgatt cagggattgt ccaagtccca tgctatgaca ggttggcgga ttggcttgat
     3301 tatgagtcag gctcccatca tcgctcaaat tatcaagagc catcagtatc ttgtgactgc
     3361 agcgtcaact gctatgcagt atggtgcggt tgaggccttg aaaaatggta aggatgacgc
     3421 tcttccgatg cgggcggagt acgtcaagcg tagggattac atcatcgaga agatgacgga
     3481 cttgggcttt aaaattatca agcctgatgg agcattttac atctttgcaa aaattcctgc
     3541 gggttacaat caagattcct ttagcttctt gcaagacttt gcgagaaaga aagcggtggc
     3601 ctttattcca ggcgcagcct tcggtcagta tggagaaggc tatgtgcgaa tttcctacgc
     3661 agcaagtatg gagaagattc aaacagccat ggctcgtttg aaggaatatc tagaagaaaa
     3721 tggaacgaat tgaaaccagg ggattagtcc tatataatcg gaattttcga gaagatgaca
     3781 agctggtcaa gatttttaca gagaaggctg gtaagcgaat gtttttcgtg aaacatgcct
     3841 ctaaatccaa gctggtagct tctatccagc ctttgaccta tgcggatttt atcgttaaaa
     3901 tcaatgatga tgggctgtct tatatcgaag attttcatca ggtacagccc tttaagaata
     3961 ttaacggtga tattttcaag cttagctatg ctacctatat cttggccttg gccgatgcgg
     4021 ccttgcagga caaggtctat gacccagctc tctttgcttt tttggtcaag accttggact
     4081 tgatggagtc aggtttggac tacgaagttc tgaccaatat ctttgaaatt cagctcttgg
     4141 gtcgatttgg gatcagtctg aattttcacg agtgtgcttt ttgtcatcgg gttggcttgc
     4201 cttttgacta ttcctacaag tacagcggtg tcttgtgtcc gcaacactat caacaagatg
     4261 agcgacgggc ttatctggat ccaaatgttc cctatctact tgatcaattt caggctattt
     4321 cctttgatga gctggaaacc atttccatca agcctgagat gaagcgaaaa ttacggtttt
     4381 ttattgacca gctgtacgag gaatatgtgg ggattcactt gaaatccaag aaatttatag
     4441 atgatttgtc ttcttggggg cagattatga aaccaagaac agaaaatgag gaaacagaat
     4501 gaaacgtatt gcagtagatg ctatgggtgg ggaccacgcc cctcaggcag tggtagaagg
     4561 tgtcaatcaa gccttggctg cctttccaga cattgaaatt caactttatg gtgatgaggc
     4621 taaaatcaag cagtatttga cagcgacaga gcgtgtcagt atcgtccata cgacggagaa
     4681 aatcaattca gatgatgagc ctgtcaaggc tattcgtcgg aagaaagagg cttctatggt
     4741 cctagcgacc aaggctgtca aggatggtca ggcagatgcg gtcttgtcag ctggaaatac
     4801 aggtgctctt ttggcggcag gcgtctttgt cgtcggtcgc atcaagaaca tcgaccgtcc
     4861 tggtcttatg tccactcttc ctactatgga cggtaaggga tttgatatga tggatttggg
     4921 ggcaaatgct gaaaatatag ctcatcatct ctatcagtat ggtattcttg gctcatttta
     4981 cgcggagcac gtgcgtggtg tcaaacaacc tcgtgtggga cttttgaaca acggtacgga
     5041 agataccaag ggcacgccag ttcaccaaga agcctataaa ctcttggcgg aagacaagtc
     5101 gattaacttt attggaaatg tggaggcacg tgagttgctc aacagcgtgg cggatgtggt
     5161 cgtgacggat ggtttcacgg gaaacgctgt gctaaaaacc attgagggaa ctgcaaaatc
     5221 catcgttggt cagttgactg gctcaatcaa gaatggcggt ctgcgtgcta aattgggagg
     5281 cttgctggtc aaaccaacct tgaaaaaggc cttgggggct atggactata aaactgcagg
     5341 tggtgctgtc ttgcttggtc tgaaagcacc tgtcatcaag gcgcacggct ctagcgatgc
     5401 ccagtcgatt ttctatacga ttaagcagac gcgttcgatt ttggaggctg gcattgttga
     5461 aaaatcagtg gccaaatttt cagtagtgga ggagagtcat gactagagag caggtctatc
     5521 agcgggttgt tgaattaatt caggatgaaa agggagaaga ttttcaagtt caacctgaat
     5581 ctactttggc agataatatt gcagcagatt cagtagaaat catggaattt gttcttaatt
     5641 tggaggatga atttcatgta gatgtcccag atgctgctat tgagcatttt gaggttctat
     5701 ctgatattgt tgattttatt tataaagaag taaaaaaacg ttcgtagttt acgggcgttt
     5761 ttctttgttt tttcctaaac aaccgtaaat ttgttgtgaa tgcgaatatt gtgtgttaga
     5821 ataaacgaaa agactagaat atttcacgaa aggcgaacat tctcatgaaa acagaccttc
     5881 tctattcagg aaaagccaaa gacatctacg ctacggctga cagcgaccag attgttgcgg
     5941 tttataagga tcaggcgacg gcttttaatg gtggtaagaa agaacagatt gtgggcaagg
     6001 gccggctcaa taatctgatt tcatctttga tttttgaaaa attgaatgaa gctggtgtcc
     6061 caacgcattt tatcaagcgt ttgtcggatt cggagcagtt gaataagaag gtggagatta
     6121 ttcctcttga ggttgttttg cgaaatgtga cagctgggtc tttctcaaaa cgctttgggg
     6181 tggaagaagg gataacctta tctactccta tcgtggaatt ttactataaa aaagatgagt
     6241 tggacgatcc ttttatcaac gatgaacaca ttgcctttct agaactggct agtcaggacc
     6301 aaatcgccta tatcaaggaa gaaacaagac gaatcaatga gttcttgaag gacttgtttg
     6361 ctcagattgg gctgactttg gtggacttca aactagaatt tgggattgac tcttctgggc
     6421 aaattttatt ggcggatgag ttttctcctg ataattgtcg tttgtgggat gcggatggca
     6481 accatctcga caaggatgtt tttcgtcggg ggctcgggga attgaccgag gtttacgagg
     6541 tcgtattggc aaaattacag gaagtgaaat aaggattgga agatatggca aagcggattt
     6601 ttgttgagaa gaaggcagca tttcagatca aggcggaggc tcttcgtaag gagttgactc
     6661 ataatttgca gttgacaagc ctgtcaagtt tgcggttagt gcaggtttac gatgtcttca
     6721 atcttgagga ggacttgtta gagcaggcca tcaagcatat ctttacagag caggtgacgg
     6781 acaaggtttt gtcggatgaa gaattaggtc tggaaggtgc agtttatttt gctattgagg
     6841 ctttgcctgg gcagtttgac cagcgggctg ccagcagtca ggaggccctt ctcttattgg
     6901 gcagtcgtca ggaggtgcgt gtcaatacag gtcagctcta tattttgaac ggtgatgtgc
     6961 aggaagaaga attagttgcc atcaagaatt atttgcttaa tcctgtggat tcgcgattca
     7021 aggatatgga tgctcctttg gtggctcagg agttttcggt atcagatact gtcattccaa
     7081 gcttggactt ttttgatagc tacggggtag aggaatttgc gtcttacaag cgtgaggctg
     7141 gtttggctat ggaagtggaa gacctgctct tcattcagga ttacttcaag tcaattggtc
     7201 gagtgccaac tgagacagaa ctcaaggtct tggatactta ctggagtgac cactgccgcc
     7261 atacgacatt tgagacagaa cttaggtcta ttgacttttc agcctcaaaa tttcaaaagc
     7321 aattgcaggc gacatatgac aagtacttgg cgatgcggac agaattaggt cggacagata
     7381 agccacagac gctcatggat atggcgacga tttttggtcg ttatgagcgg gcaaatggtc
     7441 gtctggatga catggaagtg tcagatgaaa tcaatgcctg ctcggtggaa attgaagtgg
     7501 atgtggatgg cgtgaaagag ccatggctcc tcatgttcaa gaatgaaacc cataatcacc
     7561 ctacagaaat cgagcctttt ggcggtgccg caacctgtat cggtggtgcc attcgcgatc
     7621 ctttgtcagg tcgttcttat gtttatcaag ccatgcggat ttcaggtgca ggcgatatta
     7681 cccaacctct gacagctact cgctcaggga aattgccaca gcaaatcatt tcaaaaacag
     7741 cggcacatgg ttattcttca tacggaaacc aaatcggttt ggcgacaact tatgtacgtg
     7801 aatatttcca cccaggattt gtcgcaaaac gtatggagtt gggtgccgta gtcggtgcag
     7861 ctccaaagga aaatgtggtc cgtgaaaaac cagttgcagg tgatgtggtc atcttgctag
     7921 gtggtaaaac aggtcgcgat ggtatcggtg gagcaacagg atcgtccaag gtgcagacag
     7981 tcgagtctgt ggaaacagct ggtgcggaag tccaaaaagg aaatgctatt gaagaacgta
     8041 aaatccaacg cttattccgc aatggtgccg tgactcgctt gattaaaaaa tccaatgact
     8101 ttggtgcagg aggtgtctgc gtagctattg gagaactggc agacggtctt gaaattgatt
     8161 tggataaggt tccactaaag tatgcaggct tgaacggaac ggaaattgcc atttctgaat
     8221 cacaagagcg aatgagcgtg gttgtccgtc cggaagacgt agatgccttt atcgcagctt
     8281 gtcgtcagga aaatatccat gcggttgtcg tagccaaagt gactgaaaaa ccaaacctgg
     8341 ttatgacttg gaatggacaa accattgtgg atttggaacg ttccttcctt gataccaacg
     8401 gtgtgcgcgt ggtggtggat gctaaggtcg ttgacagtcc tgtcaatcta ccagaaacac
     8461 gtacaacatc tgcccaaaca ctacgagagg acttgaaaga ccttctatca gacctcaacc
     8521 atgctagtca gaaaggtttg cagacgattt tcgactcatc tgttggtcgt tcaaccgtca
     8581 accacccact cggaggtcgt caccagctga ctccgacaga aagttcggtg caaaagttgc
     8641 ctgtccaaca gggtgtgacg acaacggctt cggtcatggc tcagggctac catccttacc
     8701 tagcagactg gtcaccttat cacggagcgg cttatgcagt catcgaagcg acagctcgct
     8761 tggtggcaac aggggctaac tggtccaagg ctcgcttctc ctatcaggag tatttccaac
     8821 gaatggataa gcaggcagag cgttttggtc agccagtagc ggctctgctc ggttcgattg
     8881 aggctcagat tcagcttggc ctgccgtcta tcggtggcaa ggactccatg tctggtacct
     8941 ttgaggactt gacagttccg ccaaccctgg ttgcctttgg tgtgacaaca gcagacagct
     9001 gcaaggttct atcacctgag ttcaaggcga ctggcgagca catttattac ctaccaggcc
     9061 agattttatc agaagacatt gatttcacct tgatcaagtc taattttaag gcttttgaaa
     9121 aatggcagag cgattatgtg attacggctg ctagtgcagt caagtatgga ggagttctag
     9181 aaagtctagc ccttatgtcc tttggtaacc aagtcggagc aagagttgag cttgccgact
     9241 ttgaaaccag cttgacaggt caacttggtg gctttgtctt cacatcgcaa gaagacattc
     9301 cagatgctgt gaaaatcggt caaaccacta cagactttac actggttgtc aatggggtca
     9361 acctttctgg acaggacttg caagtagctt ttgagggcaa actagaagaa gtttacccaa
     9421 cagagtttga acaggcgacg gagttgcagg atgttccagc agtcacaagt tcagcggtta
     9481 taaaagcaaa agaaaccgtt gaagtgcctg tagtttacat tccagttttc ccaggcacca
     9541 actcagaata tgattctgcc aaggcctttg aacaggctgg tgcaaaagtc aatcttgttc
     9601 catttgtgac cttggatgca gagagcattg aaaactcggt tgacactatg gttgacaata
     9661 ttgctaaggc gaatattcta ttctttgcag gtggattctc ggctgcggat gaaccagatg
     9721 ggtctgctaa gtttatcgtg actatcttac gaaacgccaa ggtccgctct gctattgacc
     9781 aatttatcga aaaaagaggc ctcatcatcg gtatctgtaa tggtttccag gcccttgtca
     9841 aatcgggctt gttgccgtat ggaaactttg aggaggcggg tgataccagt ccaaccctct
     9901 tctacaacga tgccaaccaa cacattgcta aaatggtgga aacgcggatt gccaatgtca
     9961 actcaccgtg gttggcaggt gtccaagtcg gcgacattca cgctatccca gtttcccacg
    10021 gtgaaggaaa atttgtagtg acggacgagg aatttgctac tttgcgtgat aatgggcaaa
    10081 tcttcagcca atacgttgac tttactggtc agccaagcat ggattctaag tacaatccaa
    10141 atggatccag ccatgccatt gagggtatta ccagtcgcaa cggacaaatc atcgggaaaa
    10201 tggggcattc ggagcgttac gaggacggtc ttttccaaaa cattccaggc aagaaagacc
    10261 aaggactctt tgtttcagcg gttcgctatt ttacaggaaa ataaatatga catacgaagt
    10321 taaatcactc aatgaagaat gtggagtttt cggcatttgg ggacatccgc aggctgctca
    10381 ggtgacctat tttggtctgc atagcttgca gcaccgtgga caagaaggag cagggatttt
    10441 agccaatgat ggcggacaat tgtgtcgcca tcgcggaaca ggcctgattg cagaagtctt
    10501 caaaaatcca gcagatttag aagccttgac aggaacggct gctattggtc atgtccgcta
    10561 tgcaacttct ggctctgctt ctatcaataa tatccagccc ttcctatttg attttgcaga
    10621 tatgcaggtg ggtttggcac ataatgggaa tttgaccaac gcggtcagtt tgaaggctga
    10681 acttgaaaaa aatggttcca ttttctcttc ttcttctgac actgagattc tcatgcactt
    10741 gattcgccgc agtcacaatc cagactttat gggaaaaatc aaggaagccc tcaatactgt
    10801 taaaggtggc tttgcttacc tgatcttgtt agaagataaa ttggttgctg ccttggaccc
    10861 taatggtttc cgtccacttt caataggcaa aatgaaaaat ggtgcctggg tagttgccag
    10921 cgaaacttgt gcttttgaag tagtgggggc tgactgggtg cgagatgtgg agcctggtga
    10981 aattgtcgtc attgatgatt caggaattca atatgatagc tatacaagag atacgcaact
    11041 ggctgtttgc tctatggagt atgtctattt tgctcgccca gattcggtaa ttcacggggt
    11101 caatgtccac acggctcgta aaaacatggg tcgtcgtttg gcacaggagt tcaaacatga
    11161 agcggatatc gtggttggtg tgccaaactc ctccctatct gcggctatgg gatttgcaga
    11221 ggaatctggt ttgccaaatg aaatggggct gattaaaaac caatataccc agcggacctt
    11281 tattcaacca acccaggaat tgcgggagca gggtgtgcgc atgaagttgt cagctgtttc
    11341 aagtgttgtc aaaggcaagc gcgtggtcat gattgacgac tctattgttc gcggtacaac
    11401 tagccgccgc attgtccagc ttcttcgtga tgcaggtgca aaagaagtcc atgtggctat
    11461 tggcagccca gaactcaagt acccgtgttt ctatggtatt gatattcaga cccgtcggga
    11521 gctgatttca gccaaccata ccgttgagga agtctgtgag attattgggg cagatagtct
    11581 gacctacctt tctcttgaag ggatgataga ggccattggt atcgaaaccg atgcgccaaa
    11641 aggcgggctc tgtgtagcct actttgacgg agaatttcca acgcctctct atgactatga
    11701 ggaagaatac cttcgtagcc tagaagagaa aacgagtttc tacattgaaa atgtcaagta
    11761 aagacagatg tgtcaacgtt acttgacaga cctgtaaact gttgaaagga atcaaaatga
    11821 caaataaaaa tgcctacgcc caatcgggcg ttgacgtcga agcgggatat gaagttgtcg
    11881 aacgcatcaa gaagcacgta gctcggacag aacgcttggg tgttatggga gccctcggtg
    11941 gttttggcgg gatgtttgat ctaaccaaac tggatgtcaa agagccagtc ttggtatctg
    12001 ggacagacgg tgtgggcacc aagctcatgc tggctatcca gtacgacaaa cacgacacca
    12061 ttggtcaaga ttgcgtggct atgtgtgtca acgacattat cgcagcaggt gcagagccgc
    12121 tttacttcct tgactacatt gcgactggaa aaaatgagcc agccaagcta gagcaggtag
    12181 tagcaggtgt ggctgaaggc tgtgtccaag ctggttgcgg attgattggc ggtgaaacgg
    12241 ctgaaatgcc tggtatgtac ggcgaggatg actatgacct ggctggcttt gccgtcggca
    12301 ttgcggagaa atcccagatt atcgatggca gtaaggtcca ggagggcgac attctcctcg
    12361 gcctagcttc cagcggcatc cactccaacg gctattccct tgtccgacgc gtttttgcag
    12421 atgtttctgg ggacgctctg ttgccagagc tcaatggcag agctcttaag gatgtcctat
    12481 tagagccgac acgtatctat gtccagcagg tgttgcctct ggtaaaagca ggtctggtca
    12541 acggcattgc ccatatcaca ggcggtggct tcatcgaaaa tgttcctcgt atgtttgctg
    12601 acaaccttgc agctgaaatt gaagaagata agattccagt cctcccaatc tttacagccc
    12661 ttgaaacata cggccaaatc aaacacgaag aaatgtttga aatcttcaat atgggtatcg
    12721 gaatggtcct ggctgtcagt ccagggaagg tggagaaagt ccgtgaattg gtaggcgaag
    12781 aagtctatga aatcggccgc attatcacta aggaagacaa gagtgtggtc atcaaatgaa
    12841 acgaatagca gtgtttgcat caggcaacgg ctccaatttc caagtcatcg cagaacagtt
    12901 tgaagtagct tttgtctttt cagaccgcag aaatgcctat gtcttggaac gagctgaaaa
    12961 actaggtgta ccaacctttg cttttgaact aaaagagttt gcggataagc aggcctacga
    13021 agaagccatt atccaactat tagaccagca ccagattgac ttggtggtct tggcaggtta
    13081 tatgaagatt gtgggaccaa ccctgttggc tcaatatgaa ggtcgtatca tcaatatcca
    13141 cccggcttat ttgcctgaat ttccaggagc ccatgggatt gaagacgctt ggaatgcagg
    13201 tgtggcagaa agtggtgtga cagttcactg ggttgacagc ggtattgaca caggacaaat
    13261 tatcaaacaa gtccgagtgc ccaggctggc tgatgatatc ctagaaacct ttgaagctag
    13321 aatacatgaa gcagagtacc aactctatcc agcagttttg gaggagttag gggcagtgaa
    13381 aaaataagga gaaatcatga caaaacgcgc actaattagc gtatcagata aaaatggtat
    13441 tgtagaattt gcccaagaat tgaccaagct tggctgggaa atcatttcga caggaggcac
    13501 aaaggttgct cttgataatg ctggtgtagc gactatcgct attgacgatg tgactggttt
    13561 tcctgagatg atggacggcc gtgttaagac cctgcacccc aatatccacg ggggtttgct
    13621 ggctcgtcgg gatgtggata gccacttgca agcagccaag gaccatgaaa ttggcctgat
    13681 tgacttggta gtggtcaacc tttatccttt caaagagacc atcttgcgtc cggacgtgac
    13741 ctacgacttg gcggtggaga acatcgacat tggcggtccg tccatgttgc gttctgcggc
    13801 taaaaaccat gccagcgtga ccgttgtggt agatccggca gattatccga cggttttagg
    13861 ggaaatagca gagcagggtc agacgaccta cccaacgcgt cagcgattgg ctgccaaggt
    13921 tttccgtcat accgcagcct acgatgctct tattgcagac tactttacca agcaagtagg
    13981 cgaagacaag cctgaaaaat taaccattac ttatgacctc aatcagccta tgcgttacgg
    14041 tgaaaatccg cagcaaaatg cggatttcta ccaaaatgcc ctgccaacag actattctat
    14101 tgcagcagct aaacagttaa atggtaagga attgtccttc aacaacattc gtgatgcgga
    14161 tgcggctatc cgtattatcc gtgatttcaa ggaccgtcca actgttgtgg cactcaaaca
    14221 tatgaaccct tgtggtatcg ggcaggcaga aactatcgag aaggcttggg attatgctta
    14281 tgaggctgac ccagtatcga ttttcggagg cattgtcgtg ctgaacagag aagtggatgc
    14341 tgcgacggct gaaaagatgc acccaatttt cttagaaatc atcatcgcac cgagctactc
    14401 ggcagaagcg ttagctattt tgaccaataa aaagaaaaat cttcggattt tagaattggc
    14461 ttttgacgca caggatgcaa gcgaagtgga aaaagagttc acaggtgttg taggtgggct
    14521 cttggtgcag gatcaggacg tggtggtgga aagcccagcg gactggcagg tggttaccga
    14581 gcgtcaaccg tccgagcaag agtgggcggc catggagttc gcctggaagt cttccaagta
    14641 tgtcaagtcc aacggcatca tcatcaccaa tgacaagatg accttgggcg tgggaccggg
    14701 gcaaaccaac cgtgtagcgt ccgtccgtat cgctatcgaa caagccaagg atcgtttaga
    14761 gggagccgtt ttggcgtcgg atgccttctt cccatttgct gataacgtgg aagaaattgc
    14821 tgccgcaggt atcaaggcta tcatccagcc aggtggctct gttcgcgacc aagactccat
    14881 tgacatggct aacaagtacg gcttgaccat ggtctttaca ggagtcagac attttagaca
    14941 ttgagagttt tggatttagc tcacttcgtg tgagcttttt tattcccgaa cattaccctg
    15001 ctttttctat caaaacccgt ctatttttgc tatactattt atagaaaatc cgtttttgag
    15061 gtgtaaaaaa tgaaactttt ggttgtcggg tctggtggtc gtgaacatgc tatcgcaaag
    15121 aaattgttag agtctgagca ggtagagcag gtctttgttg ctcctggaaa tgacggaatg
    15181 actttagatg gtatcgagtt agtcaatatc gggatctccg aacattctgc tataatcaac
    15241 tttgctaagg aaaatgacgt tgcttggact tttgtgggtc cagacgatgc tctggcagca
    15301 ggaatcgttg atgattttga acaggcggga cttaaagctt ttggccctag tcgtctagcc
    15361 gcggagctag agtggtcaaa agactttgcc aaacaaatca tggtcaaata cggcattcca
    15421 acagcagcct ttggcacatt ttccaacttc gaagaagcca aagcctacat cgaagagcag
    15481 ggtgcaccaa tcgtggtcaa ggcggacggc ttggcactgg gcaagggcgt ggtcgtggcg
    15541 gaaaccgtcg agcaggcggt cgaagcggca cgggagatgc tcttggacaa caagttcggc
    15601 gactcgggtg cccgcgtggt catcgaggag ttcttggcgg gtgaggagtt ttccctcttt
    15661 gctctggtca atggcgacca attttacatt ctgccgacag cccaggacca caagcgtgcc
    15721 tttgacggcg accaaggtcc caacacaggt ggtatggggg cttacgctcc tgttccccac
    15781 ctgcctcaaa gcgtggtgga cacagcggtt gacaccattg tcaagccgat tcttgagggc
    15841 atgattgcgg acggccggtc ttatctgggc gtgctctatg ctggcttgat tctgaccgac
    15901 caaggtccca aggtcatcga gttcaacgcc cgttttggcg acccagaaac ccagattatc
    15961 ctgcctcgcc tgacctctga ctttgctcag aacatcgacg acatcctcca caaacgcccg
    16021 acacagctga cttggctgga tagtggcgtg acgctgggtg ttgtcgtggc atcaaacggc
    16081 taccctctgg actacgaaaa aggcgtagag ttgccagcaa agaccgaggg cgacatcacg
    16141 acctactatg caggggctcg ttttgcggaa aatagcagag cactgctttc aaacggcggt
    16201 cgggtttata tgctagtcac cacagcagac accgtccaag acgcccaaga aaaaatttac
    16261 tcggagctga aaaatcaaga taccacaggc ctcttttatc ggacagatat tggaagtaag
    16321 gcagtaaaat aagggataaa gaaaaggaaa gacaagcatg aacattccaa tttccatcat
    16381 catgggttct agttccgact ggaaaaccat gaaaaaagca gccgatatgc tggacaaatt
    16441 tggcgtagcc tatgaaaaga aagtggtctc tgcccaccgc accccagacc tcatgttccg
    16501 tcacgccgaa gaagcgcgtg gtcatggctt caaggtcatt atcgcaggag cgggcggtgc
    16561 ggctcatttg ccaggtatgg tagcagccaa gacaacccta cctgtcatcg gtgttcctgt
    16621 ccaatcccgt gccctcagcg gtgtggattc gctctattct attgtgcaga tgccgggcgg
    16681 cgtgcctgtt gcgactatgg cgatcggcga agcaggtgcc accaacgcag ccctgacagc
    16741 tcttcgcatt ctctccattg aagatcaaac cattgcggct cagctggcag attttgccaa
    16801 ggaacaggaa aaaattgcgg aggcgatgac agatgacctc atctaagaca atcggaatta
    16861 tcggcggcgg tcagctgggt cagatgatgg ctatttccgc tatttacatg ggccacaagg
    16921 tgatcacgct ggatcctgcg gcggactgcc cagcctccaa ggtcagcgag gtcatcgtcg
    16981 ctccctatca cgatgtggcg gccctcaaac agctggcgga gcggtgcgat gtcctgacct
    17041 atgagtttga aaatgttgat gccgacggac tggacgcggt cattaaggac ggccagctcc
    17101 ctcaagggac tgacctcctc cgtatttccc aaaaccgcat ttttgagaag gattttttgg
    17161 caaaaaaagc tggcgtgcaa gtcgccccct acaaagtcgt cacatctagc ctagacctag
    17221 aaggactgga cctcagcaaa aactatgtct taaagacagc cactgggggc tatgacggac
    17281 atggccagaa ggtcattcga gaagaggcgg acttggtaga agctagtcag ttagctaact
    17341 ctgccgagtg tgttttggaa gagtttgtga atttcgacct ggaaatctcc gtccttgtat
    17401 ctggcaatgg ctccgactac accgtctttc ctgtgcagga aaatatccac cgcaataata
    17461 tcctttacaa aaccatcgtg cctgcccgta tttttgacga acttgctgaa aaagccaaaa
    17521 ccatggccct gcaaatcgcc gacaagctcc atttagtagg caccctctgt gttgaaatgt
    17581 ttgtggcagg gcaagaaatt cttgtgaatg aaatcgctcc tcgcccgcac aattcaggac
    17641 attattcgat tgaagcctgt gatttttcac agtttgacac ccatatacga ggcattttag
    17701 gggagcccct gccccctatc cgcctgcttt caccagcagt tatgattaac gttttgggac
    17761 aagacatgga aacagtccaa acctttcttc aagaaaaccc taccgcccat ccccactttt
    17821 atggtaaact agaagcgaag cacaatcgca aaatgggaca cgtgacggtg ttgggggaag
    17881 atgcggaaag tgtggaggag tttgaaaatt aagattgctt tctgattttt tagttattaa
    17941 aaagaggttt atgacatgcc attaccacaa aaaattcaag aagagattaa aagatattgc
    18001 aataatcact tgcctaataa tgactggtat gagaaagagt ttgattttat acatgatgtg
    18061 agtctgaaga atagaattat tagagagttt aaatcaattc ggtacgctta caagttatac
    18121 gaagggataa cggctgagga ggaacattta atttttgaaa ttcgcagtca aattttagca
    18181 tatgcctcta tatatgaagc agtagtagag tacgttttag agacatacta tagtgatact
    18241 caagtttatg atgatttggt ccatcaaaat aatgtgatga caaaaattga cataccagaa
    18301 gaaaagagga aaaaattaga aagagaatta atccat
//
LOCUS       DBNFCK010000029        18246 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.029, whole genome shotgun sequence.
ACCESSION   DBNFCK010000029 DBNFCK010000000
VERSION     DBNFCK010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 18246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18246
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.029"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            53..1693
                     /locus_tag="AC1PIW_000784"
     CDS             53..1693
                     /locus_tag="AC1PIW_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative polysaccharide biosynthesis protein"
                     /protein_id="HIP6605740.1"
                     /translation="MSEKVEMQQSKEQATMARGLAWLTAGNILSRLLGVAYVIPWYIW
                     LGEYRAEANALFSMGYQIYANFLLISTAGLPTAIAKQVAKYNVLGKEEVSLYLVREFF
                     KLMLVFGAVFAGVMYLSAPWLADASGSKEKLLPVMYSLVPPLFIFPAMSILRGFFQGR
                     HDMKPYAISQLAEQLVRVIWILAATFMIMKLGSGDYLEAVVQSTFAAFVGMIASVGIL
                     VYTLWKQGYLGKLLHAKKQKISLDTGQLIKETVRDAIPIIILGLTIQLLQFIDQVTFI
                     RVMERITDYSNSELLELYSYMAANPSKITMMIIGISLSLGSVAIPLITEKFVKKDLKA
                     ASHLVADNLQLLFIFTIPAIVGTVLLAGPLYTIFYGPSEPIAITLFIWNLFLILPLGL
                     YSVISVVIQAIFENRRAIYYFLIGMLVKIVLQVPMIYVFKVYGSFISTIFGLGIMLYL
                     FYRRIDKVLHIDERLVIKDIATISWISIVMGLVVWGIEFVLNLILPANGYVSSFIHLA
                     VAGGAGMLVFVILTLKTRQLDRLIGSRAQSLRRKLRLG"
     gene            1860..2594
                     /gene="pyrH"
                     /locus_tag="AC1PIW_000785"
     CDS             1860..2594
                     /gene="pyrH"
                     /locus_tag="AC1PIW_000785"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIP6605741.1"
                     /translation="MMKPKYERILIKLSGEALAGERGVGIDLKTVQEMAKEIQEVAES
                     GIQIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLKQLGVDTRVQ
                     TAIAMQSVAEPYIRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDANAVKFNELTHREVISRGLKIMDATASTLSMDNDIDLVVFNM
                     NEPGNIKRVVFGEPIGTTVSNSSEEK"
     gene            2729..3286
                     /gene="frr"
                     /locus_tag="AC1PIW_000786"
     CDS             2729..3286
                     /gene="frr"
                     /locus_tag="AC1PIW_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIP6605742.1"
                     /translation="MSKEIIAKAQERMNQSHQSLAREFSHIRAGRANASLLDRISVEY
                     YGSPTPLNQLAGITVPEARVLLITPFDKSILKDIERALNASDLGLTPQSDGTVIRLVI
                     PALTEETRKNLAKDVKKVGENSKVAIRNIRRDAMDEAKKAEKAKEITEDELKTLEKDI
                     QKVTDDAIKTIDKMTADKEKELLEV"
     gene            3418..4272
                     /gene="cvfB"
                     /locus_tag="AC1PIW_000787"
     CDS             3418..4272
                     /gene="cvfB"
                     /locus_tag="AC1PIW_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HIP6605743.1"
                     /translation="MNDLLAQYIVGLVTDENDQFYFVQKEGRTFALSKDEGEHSLGQS
                     VKGFAYTDMKQKLRLTTKEVGASRTSFGWGTVTEVRKDLGVFVDTGLPDKQVVVSLDI
                     LPEIKELWPKKGDRLYVKLDVDKKDRIWALPAFQEDFQKMAGPAYDNMQNQNLRAIVY
                     RLKMNGTFVYLPDNNMLGFIHPSERFAEPRLGQVLEARVIGYRAVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSAPEDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DSFGTELV"
     gene            4282..5202
                     /locus_tag="AC1PIW_000788"
     CDS             4282..5202
                     /locus_tag="AC1PIW_000788"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HIP6605744.1"
                     /translation="MLVYIAGSGAMGCRFGYQLSKTNHEVILLDNWEEHIEAIRENGL
                     KVTGDVEETVYLPIMKPTEATREADFIILLTKADQLPKMLRDIKPIIGPTTKVLSLLN
                     GLGHATTMRRYVPDESIMVGVTMWLAGLKGPGHAHLEGPGSMSVQHILGDEQSVADII
                     EMLNEAGLNVTYDNHVVSAIWQKACVNGVMNPTCTILDCTIGELFGTEAGLNLVQGIF
                     KEFIAVAKSEADGIDEEAIWKYVIDASKKASNHYPSMHQDLVQHGRKTEIDYLNGAVV
                     FKGKRAGISTPYCQMITDMIHAKESMLGLR"
     gene            5212..5667
                     /locus_tag="AC1PIW_000789"
     CDS             5212..5667
                     /locus_tag="AC1PIW_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HIP6605745.1"
                     /translation="MLIEVETIEEYLAALPENRKEVVERLHQVIVEQLPAGFEVGILG
                     GMINYYVPLAAYPDGYHCTPGEPLPFLALASQKAHIALYHMGIYMDQELNDWFVAAYQ
                     AQVPTKLDMGKSCVRMKNPKNIPYELIGELVSKMSMERYIELYEKNHRK"
     gene            5673..5888
                     /locus_tag="AC1PIW_000790"
     CDS             5673..5888
                     /locus_tag="AC1PIW_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HIP6605746.1"
                     /translation="MRRSFYSWLMTQRNPKSNEPVAILADYAFDESDFPKQSDNFDEV
                     SRFLEESASFAFSMSDFDAIWEDYLGH"
     gene            5967..6989
                     /locus_tag="AC1PIW_000791"
     CDS             5967..6989
                     /locus_tag="AC1PIW_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIP6605747.1"
                     /translation="MLEHSIDIQLKHPDDSFSLFGSNERHLRLMEQELGVVIHARTEK
                     VQVIGQEEHVEQARLVIQSLLVLVSRGLVINTPDVVTAISMAKNDEIEKFVALYEEEI
                     IKDNYGKPIRVKTLGQKLYVDSVKRHDIVFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IVLTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKEQTTRLMEREIIEIAP
                     LAYMRGRTLEDAFVILDEAQNTTIMQMKMFLTRLGFNSKMIVNGDISQIDLPRKVKSG
                     LIDATEKLSKIPQIDFVHFSAKDVVRHPVVAQIINAYEQEALAADGRASFETIGEPSR
                     KYNGEE"
     gene            complement(7659..8807)
                     /locus_tag="AC1PIW_000792"
     CDS             complement(7659..8807)
                     /locus_tag="AC1PIW_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HIP6605748.1"
                     /translation="MTTSRFQLVGSLLRPANLGEFKRQIEAREDIKYPFYDDFDGYKE
                     TEASLIQKIVAEQKENGLDIVTDGEFGRSMWHLDFLWGFDGIERYIAEHGYPFKDHDG
                     QIFETRKDIGLRITAPLSAKNHHFIDIFKQVKELAGETVTKQTIFGPAHAFNELTIFN
                     GQVGPNQVYKTRDELKVGLIAAYKEFLDQYKEAGGQIVQFDDCLWELFDPANPVPFLP
                     QDDSEALAALADEFVAINNAVIDYGHEIGLTVWTHNCRGNYESRSAAGGTYEAIAEKF
                     LRDQKYDRFFLEWDDERAGDLKALESLRDKNVEVVLGLLSSKTSDLDDEERVYKLLEE
                     ASKIIPKERLYLSHQCGFASCDSGNELAIPQQWAKIKQGQEIAEKFWS"
     gene            9006..10172
                     /locus_tag="AC1PIW_000793"
     CDS             9006..10172
                     /locus_tag="AC1PIW_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIP6605749.1"
                     /translation="MKKQSPFIIAGIVMLGVVMRAPFTALPAILIDVAAGLRVEVSSL
                     GILTSIPLIMFALCSSLAPRLAAKFGMEKLMALVLLVMVLGSGMRVLNLPALYIGTML
                     VGATIAFINVLLPSLVAANFPKKIGLYTTIYITLMGVAATVASMIAVPIVSSSSWEFF
                     ILLITGLVFMAFLIWLPNVKNNHRFASENQGNQKSSIWKNKAAIAFLIFGGLQSVLYY
                     TEITWLPTISQSVGFSKAEAGLMAGFFNMTAIPMSMIIPAVLSRQTKEMRRNIMLAIS
                     SVTLLGLFMMSLIPTNLILWSALHIILSFSNAALFPYMMLSFTLKTSNSQATAQLSGM
                     VQTGGYLIAAFGPGLLGYSYPIFGNWMPLILALAIVTLAMMWTIVLIEREDIIL"
     gene            complement(10272..10442)
                     /locus_tag="AC1PIW_000794"
     CDS             complement(10272..10442)
                     /locus_tag="AC1PIW_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003002781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HIP6605750.1"
                     /translation="MAKGFGKGFLTGVLSTVALAAGAVFTVHKTIIEPEEKKEAFIEE
                     NRKKAARKRVAH"
     gene            10511..11395
                     /gene="miaA"
                     /locus_tag="AC1PIW_000795"
     CDS             10511..11395
                     /gene="miaA"
                     /locus_tag="AC1PIW_000795"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIP6605751.1"
                     /translation="MKTKVIVVIGPTAVGKTALGIDLAQRYNGEIISGDSQQVYRKLD
                     IGTAKASPEEQAAAVHHLIDVRDVTEGYSAYEFVAEAKALIADIKSRGKLPIIVGGTG
                     LYIQSLLEGYHLGGLVDQEQVLAYRAELDCLSDEDLETMAEQAGLMVEGNSRRRIIRG
                     LELKKFGENLENTESGYEPLYICLTDDRQVLYDRINQRVDKMMAAGLLDEVSWLYQEH
                     PEAQAAMGIGYKEFFPYLEGQISLEEAIDNVKQNSRRFAKRQLTWFRNRMAVDFYQVS
                     EEAVKDRIYTAVEEFLDD"
     gene            11388..12638
                     /gene="hflX"
                     /locus_tag="AC1PIW_000796"
     CDS             11388..12638
                     /gene="hflX"
                     /locus_tag="AC1PIW_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HIP6605752.1"
                     /translation="MIETQKEQERALLVGVELQQTDNFDVSMEELASLAKTAGALVKG
                     VYTQKREKYDSKTFVGSGKLDEIAQMVEADEIDTVIVNNRLTPRQNVNLEEILGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYMLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSIRNQIHDIERQLKTVEKNRATVRERRLQSGVFKIGLIGYTNAGKSTIMNAM
                     TDKRQYEADELFATLDATTKQINLADKFNVTLTDTVGFIQDLPTELISAFKSTLEESM
                     NVDLLLHIIDASDPNHSEQEQVVLDILKDLDMLDIPRLALYNKLDKTDDSFTPSQFPH
                     VMLSTKDENAKGHIQMMVLAKIKTMFERFEVRVPLAESYKLHDLATLALIENRTYEED
                     VEVVSGYIASSNKWKLEEFYDGLS"
     gene            12622..13251
                     /locus_tag="AC1PIW_000797"
     CDS             12622..13251
                     /locus_tag="AC1PIW_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HIP6605753.1"
                     /translation="MTDYLDLAIKYGGFTSLDKVYLARKLADMTDQQKLDFITPPPSV
                     INAYFAEIYQKQGAEEATDYYLTLSQQLCLFPKEPSFAESKPFVRLNLSGKSFGFTYV
                     SADGLAQVFSEVGEEVTDSLLFEIAQVFPHYVVFVEEGKIFMKVNPFEDAELEEVETD
                     YLLTQVATGEGLVKISGFNQEEVLEVADGYAGQHYYVWSGRSAILYIKH"
     gene            13262..14191
                     /gene="rnz"
                     /locus_tag="AC1PIW_000798"
     CDS             13262..14191
                     /gene="rnz"
                     /locus_tag="AC1PIW_000798"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HIP6605754.1"
                     /translation="MQIQFLGTGAGQPSKARNVSSLALKLLDEINQVWLFDCGEGTQN
                     RILETTIRPRKVAKIFITHLHGDHIFGLPGFLSSRSFQSSEEQTDIDIYGPVGIRSFV
                     LASLKVSGTRLPYRIHFHEFDVDTVGQVLETDKFTVFAEKLDHTIPCVGYRVIQKDLE
                     GTLDAEALRAAGVPFGPLFGKIKNGQNVTLEDGTEIIASDYISPPRPGKVVTILGDTR
                     KCHASVRLAVNADVLVHEATYGKGDEKIARKHGHSTNMEAAQVAKDAGVKQLLLNHIS
                     PRFLSKDISQLRKDASTIFEQVHIVKDLEEIEL"
     gene            14188..14943
                     /locus_tag="AC1PIW_000799"
     CDS             14188..14943
                     /locus_tag="AC1PIW_000799"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIP6605755.1"
                     /translation="MRTILITGASGGLVQEMVPLLKDDFLILLGRDVAKIEQLYAYHE
                     KKAVFDVDIRDEIALTAFFDELDSQFGQIDILVNNAGYAIYDDFENFSSQQVQAMFDI
                     NTFALMTMCRLVGKRMKARRSGQIINIISMSGLIASAKSSVYSATKFAAMGFSNTIRL
                     ELAQYGVTVTTVNPGPIATSFFDQADPDGSYQESVKAFLLQPDYVAKKIVSAMGTKKR
                     DINLPWSLAAAHKLYTLFPRIADYLASTVFNLK"
     gene            complement(14987..18172)
                     /locus_tag="AC1PIW_000800"
     CDS             complement(14987..18172)
                     /locus_tag="AC1PIW_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HIP6605756.1"
                     /translation="MKLSYKKRLLNQVLLASTVLLAASLAQGTVFANTEEIPPTTSET
                     VTPLPEETPITKTSTSEATDNLVEGKETEKQTEEIADTSPTPVSTEEDTTSSEPNAEE
                     TTLRTANNDNQDTTEEKSAVPTIDTVTLETKTVYLSEAVTITESITLPDTTEKIEWTL
                     DGKSISEWKTWNLKEGDFTGDTFITVEESRQDNQLHLNIQLAALFGEDLSKRTPSNIR
                     RTYRHFIKNMLLEGTSADGNLIISKTLHFRPYEAYRTHEEMLTEIEETKNNAATDRLV
                     RIESIGQSAEGRDIKMAVVAKDQASIDKYLTETTPLMLTQPDQMLKQLQAGTFDYKLP
                     ILINNTHADEQPGIDVVTSLFKEFAQKDTITFPSTDADGNPVTLHLKVTDLLDKFIFL
                     FNFTENPDGDVKNLRSLVNGLDPNRDTGFQVNPETQAIVRQIHKWNPISVLDIHGFVS
                     GFLIEPATPPHDPNFEYDLLADIMLEKAHEMGRAGIANSKYERYTIPKVHWGDGWDDS
                     FSGYTAVYAMYHGILGHTIEIPEGNQESFKAGFFAVLGGVHNMATKPDSLMEMRLKYY
                     SRGVNKVEDPKAESELVGPDGAVVGRVKKDQPKFFPDYYVIPMTLDKHNDMQEAFKMI
                     EYFNRNGVVVKELTEDVGNFRKGDLVVDMAQAKRGFANHVLYAGSDESAWGAMYAELV
                     VNFPDMKGFSAKAVFEENTFSGKLGSITWTKAPRTTEIDFKAPYYVVANTSESAVQAI
                     NQAIKSGAKVYLTDDGYIMETNQFSHLLDTYALYGEPLYKKPLGQELKAMKVYAPSHS
                     YSWAGDFAILANAALAVERMGFEIVNSADEADAIILESDQFDASVFGKKPIIIVGGVA
                     MQKLEELGILAGFNAEQFTDGGDYEGLMRAIIDDKDPLTSGYAMNGLFYSNSGNWIEG
                     IPEGFKTLVKIADKDYYIAGWWPGHDKLANKIVAIAGNYQDQPVFIYAGNPTNKVHPV
                     HFFRWVSNALFGSQLASLEDLPAVEILVPQPEMPKNILDEAPKTTTVVHTTKSTEAKQ
                     LPQTGEKTNYIAIALGSLLLGSIALRRKERS"
BASE COUNT         5081 a         3524 c         4204 g         5437 t
ORIGIN      
        1 attaataatt gagtttttcc tgtcttatat ttacatagaa agtaatagaa aaatgtccga
       61 aaaagttgaa atgcagcagt ccaaagaaca ggcgacaatg gccagaggct tggcttggtt
      121 gactgctgga aatattctta gtcgcctgct tggtgttgct tatgttattc cctggtatat
      181 ttggttaggg gaatatcgag ctgaagccaa tgccctcttt agtatggggt atcaaatcta
      241 tgccaacttc ttattgattt caacagctgg tcttccgaca gctattgcta aacaagtagc
      301 taaatacaat gtcttgggta aagaagaggt gtcgctttac ttggtcagag aattcttcaa
      361 gctcatgttg gtctttgggg ctgtctttgc aggggtcatg tatctaagtg ctccgtggtt
      421 ggcagacgca tcggggtcga aagaaaagtt acttccagtc atgtatagtc tagtcccgcc
      481 tctctttatc ttcccagcca tgagtatctt gcgtggtttt ttccaaggtc ggcacgatat
      541 gaagccctat gcgatcagcc agcttgcaga gcaactggta cgggttatct ggatactggc
      601 agcaacattt atgattatga agttgggtag cggtgactat cttgaagcgg ttgtccaatc
      661 tacctttgca gcttttgtcg ggatgattgc tagtgttggg attttagttt atactttatg
      721 gaagcaaggt taccttggta agttgcttca tgctaagaaa caaaagattt ctttggatac
      781 aggtcaactt attaaggaaa ctgttcgaga tgcgattcca attatcattt taggtctaac
      841 gattcaatta ttgcagttta ttgaccaagt cacgtttatt cgtgttatgg aaagaattac
      901 ggattacagt aattcggaac ttttggaact ttattcatat atggctgcca atccaagtaa
      961 gattacgatg atgattatcg ggatttcact tagtttggga agtgttgcta ttccattgat
     1021 tacagagaaa tttgtcaaga aagatttgaa ggcggcgtct catttagtgg cggataatct
     1081 acaattactc tttatattca ctataccggc aattgttgga acagtcctgt tagctgggcc
     1141 actttatacg attttttacg gaccgtcaga gcctatcgcc attactctat ttatttggaa
     1201 cttattttta attttacctc tgggactata ttctgtcatt agtgtggtca ttcaagcgat
     1261 atttgaaaat agaagagcca tttattattt cttgattggg atgctagtga aaatcgtttt
     1321 acaagtacca atgatctatg tctttaaggt atatggtagc tttatctcaa cgatatttgg
     1381 gttgggaatt atgctgtacc tcttctatag gcgaattgac aaggttctgc atattgatga
     1441 aagactagtg ataaaagata ttgcgaccat tagttggatt tctatcgtaa tgggactcgt
     1501 tgtatggggg attgaatttg tcttgaatct tatccttcca gcaaatggct atgtatctag
     1561 ctttatccac ttggctgttg caggtggagc aggtatgctt gtctttgtca tcttgacatt
     1621 gaagactcgt cagctagatc gtttaattgg aagtcgggct cagagtttac ggaggaaatt
     1681 gcgcctaggc tagtttgttt taaagatctc tattttctac tattgaaaag ctgggaaaaa
     1741 gaggcgtttt agaatgtgag aaggaaagtt cgttgaggat tttccttttt tcttggagat
     1801 ttcagtataa aaaaaacggt ttttgtggta gaatagtagg gataaaaaag aggagagaga
     1861 tgatgaaacc taaatacgaa cgtattctaa tcaaactatc tggtgaagcc ttggctggtg
     1921 aacgtggtgt cggtattgac cttaaaactg ttcaggaaat ggcaaaggaa attcaggaag
     1981 tcgcagaatc aggtattcag attgcccttg ttattggtgg tggtaacctt tggcgtggag
     2041 agcctgctgc tgaagcgggt atggatcgag ttcaagcaga ctatacaggc atgttgggga
     2101 ctgttatgaa tgctcttgta atggctgatt cccttaaaca gcttggtgtg gatacacgtg
     2161 tgcagacagc gattgcgatg cagtcagttg cagagcctta tattcgtggt cgtgctcttc
     2221 gtcatcttga aaaaggtcgc attgttattt ttggagctgg tattggttct ccgtacttct
     2281 caacggatac gacagcggct cttcgtgctg cagaaattga agcggatgcg attttgatgg
     2341 ctaaaaatgg tgtggatggc gtctacaatg ctgatccgaa gaaagatgct aatgcagtta
     2401 aattcaatga attgacccac cgtgaagtga ttagtcgtgg tttgaaaatc atggatgcga
     2461 cagcatcaac tctctcaatg gacaatgata ttgatttggt cgtgttcaat atgaatgaac
     2521 caggaaatat taaacgtgtt gtcttcggtg agccaatcgg tactaccgtt tcaaattctt
     2581 cagaagaaaa ataaatcata tcgtcattta gtttttcttt tattacttcg ttaattcgcc
     2641 ttgtctaact tcggttatgc ctgtggctcg ttacctagta ctaaaagtaa actaaaagac
     2701 tgtaaaaaaa tacaaagaag gaagtcttat gtctaaagaa attattgcta aagcgcaaga
     2761 gcgcatgaac caatctcatc agagtttggc acgtgaattc agccacattc gtgctggccg
     2821 tgccaatgct agtttgttgg accgtatttc agttgagtac tacggttctc caacgccatt
     2881 gaaccagttg gctggtatta cagtgccaga agcgcgtgtt ctattgatta ctccgtttga
     2941 taagtcaatc ttgaaggata ttgagcgtgc tttgaacgct tcagatcttg gtttgacacc
     3001 gcaatctgac ggtactgtta tccgtttggt tattcctgcc cttacagagg aaactcgtaa
     3061 gaacttggcg aaggatgtga aaaaagtcgg tgaaaattca aaagttgcca tccgtaatat
     3121 ccgtcgtgat gccatggatg aagctaaaaa agctgaaaaa gcaaaagaaa tcacagaaga
     3181 cgagttgaag acgcttgaaa aagatattca aaaagtaaca gatgatgcta tcaagacaat
     3241 tgataaaatg actgccgaca aagaaaaaga attgttggaa gtttaatcaa cacgttttta
     3301 gactacaaaa atagatcatc actgggctgt ctaacttcca gtggtgaacc aataagaaat
     3361 ctaatggggg gagttagaca agacttcccc cactttttaa tcagaaagaa gaaaatcatg
     3421 aatgatttat tagcacagta tatcgttggt ttagtgactg acgaaaacga tcagttttac
     3481 tttgtccaaa aagagggtag aacctttgct ctttctaagg atgaaggcga acatagtcta
     3541 ggtcaatctg ttaaaggatt tgcctatacc gatatgaaac aaaaactccg tttgacaact
     3601 aaggaagttg gggcgagtcg taccagcttt ggttggggaa ctgtcacaga ggtacgtaag
     3661 gatttgggtg tctttgtgga tacaggtcta ccagacaagc aagtggttgt ttccttggac
     3721 attttgcctg aaatcaagga actctggccg aaaaaagggg atcggttgta cgtcaaatta
     3781 gacgtcgata agaaggaccg catttgggct ctgccagctt ttcaagaaga ttttcagaaa
     3841 atggctggac ctgcttatga taatatgcaa aaccaaaatc tgcgagcgat tgtctatcgt
     3901 ttgaaaatga atggtacttt tgtttatttg ccagacaaca acatgcttgg ttttatccac
     3961 ccaagtgaac gatttgcgga accacgtctg ggtcaggttt tggaggctcg tgtcatcggc
     4021 taccgtgcag ttgatcggac cttgaacttg tctctcaagc cacgttcatt tgagatgctg
     4081 gaaaatgatg ctcagatgat attgacttat ctggaaagca atggaggttt catgaccttg
     4141 aacgataagt ctgctccgga agacatcaag gccacctttg gtatttccaa agggcaattc
     4201 aaaaaagctc ttggtggttt gatgaaggct gggaaaatta aacaggattc ttttggaaca
     4261 gagttggtct agggggcgtc tatgctagta tatattgctg gtagtggggc aatggggtgt
     4321 cggtttggtt atcaattgtc taagacaaat catgaagtga ttttgctgga taattgggaa
     4381 gaacatatcg aagcgattcg agaaaacggc ttgaaagtga ctggagacgt agaggaaact
     4441 gtatacttac ccatcatgaa accgaccgaa gcaacgagag aagcagattt tattattcta
     4501 ctgacaaagg ctgatcagtt gccaaagatg ttgcgggata ttaaaccgat tatcggtcca
     4561 actaccaagg ttttgagtct cttgaatggt ttggggcatg cgacgaccat gcgtcggtac
     4621 gtgccagatg aaagtattat ggttggggtg accatgtggt tggcgggact aaagggtcct
     4681 ggacatgctc acttagaagg tccgggttct atgtccgttc agcatattct tggtgatgag
     4741 caatcagttg cggatattat tgaaatgctg aatgaagctg gactaaatgt gacctatgac
     4801 aatcatgttg tcagtgctat ttggcagaaa gcctgtgtca atggtgtgat gaatccaact
     4861 tgtaccattt tagactgtac aattggtgaa ttatttggta cggaggctgg actaaatcta
     4921 gtacagggga ttttcaaaga atttattgct gttgctaagt ctgaggcaga tggaattgac
     4981 gaagaagcta tttggaaata tgttattgat gcttcgaaga aggcttccaa tcactatcca
     5041 tctatgcacc aggatttggt ccaacatggt cgaaagacag aaattgatta tctgaatgga
     5101 gcggttgttt ttaagggcaa acgtgcaggt atctctacgc catactgtca gatgataaca
     5161 gatatgattc atgccaagga aagtatgttg gggttgagat aggagaaagt catgctgatt
     5221 gaagtggaaa cgattgaaga atatttggct gccttgcctg aaaatcggaa ggaagtagtt
     5281 gaacggctcc atcaagtgat tgtggaacag ttaccagctg gttttgaagt aggaatcttg
     5341 ggaggtatga ttaactatta tgtgccgctt gctgcctacc ccgatggtta tcattgcaca
     5401 ccgggagagc ccttgccatt ccttgctttg gcatcacaaa aggcacacat cgccctctac
     5461 cacatgggaa tctatatgga ccaagaattg aatgattggt tcgttgctgc ttatcaagca
     5521 caggtgccta ccaaactgga tatgggcaag tcctgtgttc gtatgaaaaa tcccaagaat
     5581 attccttatg aattgattgg ggaattggtt agtaagatgt ctatggagcg ctatatagag
     5641 ctctacgaaa aaaatcatag aaagtagatg tagtgagaag aagtttttat tcttggttga
     5701 tgacccagcg caacccgaaa agtaatgaac cagtagctat cttggcagat tatgcctttg
     5761 atgaatctga ttttcctaag caatcagata atttcgatga agtcagtcgt tttttggagg
     5821 aatcggccag ctttgccttt tccatgtctg attttgatgc gatttgggaa gattatttgg
     5881 gacattgatg gataaaatag tacaaaatag ccttgtttgg gctatttttg ttataataga
     5941 gaaaagcaca ggaaaaggag caactcttgc tagaacattc aattgatatt caactcaagc
     6001 atccagatga ttcatttagc ctatttggtt cgaatgaacg ccatctgaga ttgatggaac
     6061 aggaactagg tgtggttatt catgcccgta cggaaaaagt gcaggtgatt ggacaggaag
     6121 aacatgtaga acaggcgcgt cttgttatcc agtctctcct tgtcttggtg agtcgtggtt
     6181 tagttatcaa tacgccagat gtggtcacag ctatttccat ggcaaaaaat gatgagattg
     6241 agaaattcgt tgccctctat gaagaagaga ttatcaagga caactatggc aaacccattc
     6301 gcgtaaagac ccttggacaa aaactctatg tggacagcgt aaagcgtcat gatatcgtgt
     6361 ttggaattgg accagctggt acaggaaaga ccttcttggc ggttactcta gctgtaacag
     6421 ccctcaagcg gggacaggtc aagcggattg tcctgacccg tcctgcggta gaagcaggtg
     6481 aaagtttagg ctttttacca ggggacttaa aagaaaaagt agacccttat ttgcgtcctg
     6541 tctatgatgc cctctaccag attttgggca aggagcagac cacgcgccta atggagcgcg
     6601 agattatcga gattgcaccg ctggcctata tgcggggtcg gaccttggaa gatgcctttg
     6661 tcatcttgga tgaggcgcaa aatacgacca ttatgcagat gaagatgttc ctgacccgtc
     6721 tgggctttaa ttccaagatg attgtcaatg gggatattag tcagattgac ctgccacgca
     6781 aggttaagtc aggcctgatt gatgcgacag agaaactaag caaaattccg cagattgatt
     6841 ttgtacattt ttcagccaag gatgtggttc gtcacccagt tgttgcccag attatcaatg
     6901 cctatgagca ggaggctctg gctgcggatg gtcgagcaag ttttgaaacg attggggagc
     6961 ctagtcggaa atacaatggc gaagagtagt tgtttcattg gctgatatat aaaagaattg
     7021 tgatgctagt tacatttaat atacaataaa aagcccaaat ggacgatact gtaagcgatg
     7081 aaacaaacag gaacttagtc caaataagcg acttacagca tatcgtaaaa tcacatcgta
     7141 cagcaaatat caactatttg tcacaagtta taccaatcct attgcccgac tcccttaaag
     7201 cattgtcaca acgttgcttt gtcaacggta tcatataaat ctattaaaat attgctagta
     7261 cttcaagttg gattagagat taaatcttaa cgacattttc gaaccagccc tcattatagc
     7321 ggatagagat cataaaacct ataactttat ggattgttac caataaaaag gctggttcta
     7381 tctgattcgg attcgagatg ggaaaagcag catgaaaccg agttttactc cctttctttt
     7441 cgcatgttcg gcaccgataa gtatgaaacc ctcgtggcca attgtgataa acctgtagac
     7501 tatataaaaa agcctccctt tgtgttgtcc gaacataaaa tcccaagtat tacatcggaa
     7561 aaatacttgg gattagatat catagattaa ctgatcagag ttaacttaga gacactgatt
     7621 gatgacaggg cttttatgca cgaataacaa gaagtggctt aagaccaaaa cttctctgca
     7681 atttcttggc cttgtttaat ttttgcccac tgttgcggga tggccaattc attacctgaa
     7741 tcacatgaag cgaatccaca ttgatgtgac aagtagagac gctcttttgg aataatcttg
     7801 cttgcttcct cgagtaattt gtaaacacgt tcttcatcat ccaaatcaga agttttactt
     7861 gaaagtagac caagaacgac ttcgacattc ttgtcacgca agctttccaa agcttttaaa
     7921 tcacctgcac gttcatcatc ccactcaagg aagaatcgat cgtatttttg gtcacgcagg
     7981 aatttttcgg caatagcttc gtaagttccg ccagctgcag agcggctttc atagttacca
     8041 cggcagttgt gcgtccagac ggtcaagcca atttcatgtc cgtaatcaat aacagcatta
     8101 ttgatagcaa caaactcgtc agccaaagcg gccaatgctt cagaatcgtc ttgtggcaag
     8161 aaaggtacag ggttagcagg gtcaaataat tcccaaaggc agtcatcaaa ttgaacgatt
     8221 tgtccaccag cttccttgta ctggtctaaa aattccttat aggcagcgat gagcccgact
     8281 tttaattcat cacgagtctt gtagacctga tttggtccaa cttgtccatt gaaaattgtc
     8341 aattcattaa aggcatgggc tggaccgaaa atagtttgtt tggttacagt ttctcctgct
     8401 aattctttca cttgtttgaa aatatcaatg aaatggtgat tttttgcaga aagtggagca
     8461 gtaatacgca atccaatatc cttacgtgtt tcaaaaatct gtccatcatg gtctttaaat
     8521 ggataaccat gctcagcaat gtaacgctcg ataccgtcga agccccagag aaaatctagg
     8581 tgccacattg aacgaccaaa ttctccatct gtcacaatat ccaaaccgtt ttctttttgt
     8641 tctgcaacaa ttttttggat gagtgaagct tcagtttctt tgtaaccatc gaagtcatca
     8701 tagaatgggt atttgatgtc ttcacgagct tcgatttgtc gtttaaattc tcctaagttt
     8761 gctggtcgaa gaagtgaacc gactaattgg aatcttgaag ttgtcatatg tgtgtctctc
     8821 tttcgtttta gtgatgaacc tattctaact cataagctat catttgaaaa atagttattt
     8881 tcatggcaaa tccatagtta aaaactataa cttgaaaaaa tagaaggaaa taactttcta
     8941 tcatttgtca actatggtat aatcaaaagt gctgaaatcc gaacattagg agacttagga
     9001 gaaaaatgaa aaaacaatca ccatttataa ttgcaggaat cgtcatgcta ggggtcgtga
     9061 tgcgtgcccc ctttacagct ttgcctgcta ttttgataga tgtggcagca ggactgagag
     9121 tagaagtgag ttcattagga attttaacct ctattccgct catcatgttt gctctctgtt
     9181 catccttagc accgcgtctt gcggctaagt ttggcatgga aaaactaatg gccctagtct
     9241 tactggttat ggtgctgggt tcaggaatgc gagttctcaa cttaccagcc ctttacattg
     9301 gtacaatgct tgttggtgct acaatagcat ttattaatgt tttgctacca agtttggttg
     9361 cggctaattt tccaaagaaa attggtctgt atacgacgat ttatatcact ctcatggggg
     9421 tggcggcaac agttgcatct atgattgctg ttccaattgt atcctctagt tcttgggaat
     9481 tttttattct attaattaca ggattggtgt ttatggcttt cctgatctgg ttgcctaatg
     9541 taaaaaataa ccatcgattt gcaagtgaga accaaggaaa ccagaaatca tctatctgga
     9601 aaaataaagc agcgattgcc tttttgatat ttggtgggct acaatcagtc ctctactata
     9661 cagagattac ctggttgcca actatttctc aatcagttgg ctttagtaag gcagaagcag
     9721 ggctaatggc tggtttcttc aatatgacag ctattcccat gtctatgatt atcccagctg
     9781 ttctttctcg tcagacaaaa gaaatgcgtc gaaatatcat gctggctatt tcatctgtta
     9841 ccttacttgg tttgttcatg atgtcattga ttccgaccaa tctcattctt tggtcagcac
     9901 ttcacattat tttaagtttt tcaaatgcag ccctcttccc ttatatgatg ttgagcttta
     9961 ctttgaaaac aagcaacagc caggctactg cccagttatc aggaatggtg cagacaggtg
    10021 gctatctcat tgcagcattt ggaccaggcc ttctcggtta cagctatcca atcttcggaa
    10081 actggatgcc attgattctt gctctggcta tcgtcacact tgccatgatg tggactattg
    10141 ttctcattga aagagaagat attatcttat aaagtataat cccttaagaa ggctggcatt
    10201 tttctgtgaa aatggcagag cttctaaggg attttttgtc aaacaaaaaa agctggacat
    10261 ttgccagctt tttaatgtgc cacacgttta cgagcagctt ttttacgatt ttcctcaatg
    10321 aaagcttctt tcttttcttc tggttcaatg attgttttgt gaacagtgaa gactgcgcct
    10381 gctgcaagag caactgtaga aagaacacct gtcaagaaac ctttaccaaa acctttagcc
    10441 atagttattt ctcctttact tgtgttataa tagattttag cgaaaaaaca aaatattttc
    10501 aaggaaaaag atgaaaacta aagttattgt ggtcatagga ccgacagcgg taggaaaaac
    10561 tgcccttggc atagacttgg cccagcgcta caatggagaa attatcagcg gtgatagcca
    10621 gcaagtctat cgcaaactgg atattggtac ggcaaaagct agtcctgaag agcaggctgc
    10681 tgcggttcat cacttgattg atgtccgaga tgtgaccgag ggctattcgg cttatgaatt
    10741 tgtagcagaa gccaaggctc tgattgctga cattaaaagt cgtggcaagt tgcctattat
    10801 cgtgggtggg acagggcttt acattcagag cttgcttgaa ggctaccatc tgggcgggct
    10861 ggttgaccag gagcaggtac ttgcttatcg ggcggaactc gactgcttgt ctgacgagga
    10921 tttggaaaca atggcagagc aggcaggttt gatggtcgag ggaaacagcc gacggagaat
    10981 cattcgtgga ctggaattga aaaaatttgg cgaaaactta gaaaatacag agtcaggata
    11041 tgagcctctt tacatctgcc tgacggatga cagacaggtt ctttacgacc gcatcaatca
    11101 gcgagtggat aagatgatgg cggcgggctt actggatgaa gttagctggc tctaccaaga
    11161 acacccagaa gctcaagctg ctatgggaat tggctacaag gagtttttcc cctacttaga
    11221 aggtcaaatc agcctagaag aagcaattga taatgtcaaa caaaatagcc gccgctttgc
    11281 caaacgccaa ttaacctggt ttagaaaccg catggcagtc gatttttacc aagtatcaga
    11341 agaagctgtt aaggatagga tttacaccgc tgtggaggaa tttttagatg attgaaaccc
    11401 agaaagaaca agaacgtgcc ctcctggtcg gagttgaatt gcagcagaca gataattttg
    11461 acgtgtccat ggaagagttg gcaagcctgg ctaagaccgc tggggccctc gtcaaaggag
    11521 tatatacgca gaagcgggag aaatatgaca gcaagacttt tgtcggttct ggtaagctgg
    11581 acgaaattgc ccagatggtc gaagctgacg agattgatac tgtcatcgtc aacaatcgat
    11641 taacaccccg ccagaatgtc aatctggaag agattttagg tgtcaaggtc atcgaccgta
    11701 tgcagctgat tttggatatt tttgccatgc gggcacggag tcacgaagga aaactgcagg
    11761 tccacctagc ccagctcaag tacatgttgc cacgcttggt tggacagggc atcatgctca
    11821 gccgtcaggc aggtggtatc ggctctcgtg gaccaggtga aagccagttg gagctcaacc
    11881 gtcgaagcat tcgcaaccag attcatgaca ttgagcgcca actcaaaaca gtggagaaga
    11941 accgagcgac tgtccgtgaa cgccgtttgc aatcaggcgt gttcaaaatc ggcttgattg
    12001 gctataccaa cgcgggtaag tccactatta tgaatgccat gacggacaag cgacagtacg
    12061 aggctgatga actttttgcc acactagatg ccacaaccaa gcagattaac ttggcagaca
    12121 aatttaatgt gaccttgacc gatacggttg gtttcattca ggacctgccg actgagttga
    12181 tttctgcctt caagtccacc ttggaggagt ccatgaacgt ggatctcttg ctccatatca
    12241 tcgacgcctc tgaccccaat cacagtgagc aggagcaggt ggtcttggac attctaaaag
    12301 acctggatat gctggacatt ccacgtctag ctctctacaa caagctggac aagaccgatg
    12361 acagctttac ccccagtcag tttcctcatg tcatgctgtc cactaaggat gaaaatgcca
    12421 aaggccatat tcagatgatg gtcttggcca agataaagac catgtttgaa cgatttgagg
    12481 tcagggtgcc acttgctgaa tcttataaat tgcatgattt agctactctg gctctgattg
    12541 aaaatcggac ctacgaagag gatgtggaag tggtatcagg ctatatcgct tccagcaata
    12601 aatggaagtt ggaagaattt tatgacggat tatcttgatt tggcaatcaa atacggtggt
    12661 tttaccagcc tggacaaggt ctatctggct aggaaattgg ctgatatgac tgaccaacaa
    12721 aaactagatt ttatcacgcc accgccttcg gttatcaatg cctattttgc ggaaatctac
    12781 cagaaacaag gggcagaaga ggcgacggac tactatctca ctctttctca gcagctctgc
    12841 ctttttccaa aagaacccag ctttgctgaa agcaagccct ttgtccgcct gaatttatct
    12901 ggtaagtctt tcggatttac ctatgtgtcg gcggacggtc tggcccaggt tttttcggaa
    12961 gtaggagagg aagtgacgga tagcttgctc tttgaaatcg cccaagtctt tccccactat
    13021 gtcgtctttg tggaggaggg caagattttt atgaaagtca atccctttga ggacgccgag
    13081 ttagaggagg ttgaaacaga ctacctttta acccaggtgg caaccggaga aggcttggtc
    13141 aagatttcag gctttaatca ggaagaagtg ctggaagtgg cggacggcta tgctggtcag
    13201 cattactatg tctggtcggg tcgctctgca attttatata taaaacatta gaaagtaaac
    13261 aatgcaaatt caatttttag gtacgggggc tggtcagccc tccaaggctc gaaatgtatc
    13321 cagtttggcc ttgaaactct tggatgaaat caatcaggtt tggctctttg actgcggtga
    13381 gggcactcag aatcggattt tggaaacgac cattcgtcca cgcaaggtgg ctaaaatctt
    13441 tattacccac ctgcacggtg accatatttt tggcttgcct ggtttcctgt ctagccgttc
    13501 tttccagtct agcgaggagc agacagatat tgatatttat ggacctgttg gtatccgttc
    13561 ttttgtcttg gcaagtctta aagtatcagg gacacgccta ccttatcgca ttcattttca
    13621 tgagtttgat gttgatacgg taggtcaggt cctagaaaca gacaaattca cggtattcgc
    13681 tgaaaagctg gaccatacca ttccttgtgt cggctatcgt gtcatccaga aagatttgga
    13741 agggacattg gatgcagagg ctctgcgcgc ggcaggtgta ccatttggtc ccctattcgg
    13801 taaaatcaaa aatggtcaaa acgtgacgct agaagatggg actgaaatta tcgcgagtga
    13861 ctatatttct cctcctcggc ctggtaaggt agtgaccatt cttggtgata cgcgcaagtg
    13921 ccatgccagt gtacgcttgg ctgttaatgc tgatgtgctg gtgcatgagg cgacttacgg
    13981 aaaaggcgat gagaagattg ctcgcaagca cgggcattcg accaatatgg aagccgctca
    14041 ggttgccaag gatgcaggtg tcaagcagct cttgcttaat catatcagtc cacgattttt
    14101 atccaaggat attagtcaat tacggaaaga tgcaagtact attttcgagc aggttcatat
    14161 tgtcaaagat ttagaggaaa ttgagctatg agaaccattc taatcacagg tgcctctggt
    14221 ggtttggtac aggaaatggt gccattgctg aaagatgatt ttttgatttt actaggtcgt
    14281 gatgtggcaa aaattgagca gctctatgct taccatgaaa aaaaggctgt ctttgatgta
    14341 gacatccgag acgaaatagc tctgacggca ttttttgatg aactggatag tcagtttggt
    14401 cagattgata ttctagtcaa taatgcaggc tatgccattt acgatgattt tgagaatttt
    14461 tccagccagc aggtgcaggc catgtttgat atcaatactt ttgctctgat gaccatgtgc
    14521 cgtttagtag gtaaacgaat gaaggcaaga cggagcgggc agattatcaa tatcatttcc
    14581 atgtcaggct tgattgcttc agctaagtcc tcggtctatt cagcgaccaa gtttgcggcc
    14641 atgggttttt ctaataccat tcgcttggaa ttagcccagt atggtgtgac agttacaacg
    14701 gtcaatccag gtccaatcgc aaccagcttt ttcgatcagg ctgacccgga tggaagctat
    14761 caagagagcg tcaaggcttt cttgctgcaa ccagactatg ttgccaagaa gattgtttca
    14821 gctatgggga cgaagaaacg agacatcaat ctgccttggt cactagcagc tgctcataaa
    14881 ctctatactc tctttccaag aatagcagac tacttggcca gtacagtatt taatttaaaa
    14941 taagcataga aaaacgccga aataaaactc ggcgttttcg tgtagactag ctacgctctt
    15001 ttctacgtaa ggcgatactg cctagaagta ggctacctaa tgcgatagct atatagtttg
    15061 ttttttcacc tgtttgaggt agttgttttg cctcggtaga cttggtggta tgtactactg
    15121 tagtagtttt tggcgcctca tccagaatat tttttggcat ttcaggttgt ggtacaagaa
    15181 tttctacagc tggtaagtct tctaagcttg ctaattgact accaaatagg gcgttggaca
    15241 cccaacggaa gaagtgaaca gggtggacct tgtttgttgg attacctgca tagataaaga
    15301 caggttggtc ttgataattt ccagcgatgg cgacaatttt attggctaat ttgtcatgac
    15361 caggccacca acctgcgata taataatctt tatcagcgat tttaacaagc gttttaaatc
    15421 cttcaggaat gccttcaatc caattaccgg agttggaata gaaaagtcca ttcatggcgt
    15481 agccacttgt taatggatct ttatcatcaa taatagcccg catgagacct tcatagtcac
    15541 caccatctgt aaattgttcc gcattgaaac ctgctaagat tcctaattcc tccagttttt
    15601 gcatggcgac accacctacg atgataattg gtttcttacc aaatacactg gcatcgaatt
    15661 ggtctgattc gagaatgata gcatcagcct catctgcgct attgacaatt tcaaagccca
    15721 ttcgttctac cgcaagtgca gcatttgcaa gaatggcaaa gtcgcctgcc caactataag
    15781 agtgagaagg agcgtacacc ttcattgctt tcaattcttg tccaagaggc ttcttataga
    15841 gaggctcgcc gtatagagca taggtgtcca gtaagtgact aaattggtta gtttccatga
    15901 tgtagccatc gtctgtaagg tagaccttag cgcctgattt gatggcttga ttaatggcct
    15961 gaacggcgct ttcagaggtg tttgcaacga cataataagg cgctttgaaa tcaatttccg
    16021 tagtacgtgg agcttttgtc catgtgatgc tacctaattt gccgctaaag gtgttttctt
    16081 caaatacagc cttggcgcta aagcctttca tatctgggaa gttgaccacc aattcagcat
    16141 acatggctcc ccaagcagat tcatcagaac cagcgtagag gacatggtta gcgaatcctc
    16201 gtttggcttg agccatatcg acaactaggt cgcccttgcg gaagtttcca acatcttcag
    16261 ttaattcctt gacaacaacc ccattacgat tgaaatattc aatcatctta aatgcttctt
    16321 gcatatcgtt gtgtttatcg agcgtcatag gaatcacata gtagtctggg aagaattttg
    16381 gctggtcttt tttgacacgt ccgacaacag ctccgtctgg tccgactagt tcagactctg
    16441 cttttggatc ttctacctta ttgacaccgc gtgaatagta tttgaggcgc atttccataa
    16501 gactgtctgg tttggttgcc atattgtgaa caccgcccaa aacagcaaag aatcccgcct
    16561 tgaatgattc ttgatttcct tctggaattt caatcgtgtg tccaagaata ccgtggtaca
    16621 ttgcatacac cgctgtatag cctgagaagg aatcgtccca accatctccc cagtggactt
    16681 tagggatggt gtagcgttca tatttagaat ttgcaatacc tgctcggccc atttcatgag
    16741 ctttttccag cataatatcg gctagtaaat catattcaaa gttcgggtca tgtggcggag
    16801 tggcaggctc gatgagaaat ccagatacaa aaccatgaat atctaaaaca gatattggat
    16861 tccatttgtg gatttgacga acgatggctt gtgtttctgg attcacttgg aaaccagtat
    16921 cacggttagg gtccaaacca tttactagtg aacgcaagtt tttaacatcg ccatcaggat
    16981 tttctgtgaa attgaacaag aagataaatt tatctaacaa atctgtcact tttaaatgaa
    17041 gagtaacagg atttccatcg gcatctgtag atggaaaggt gatggtatct ttttgtgcaa
    17101 attctttgaa taggctagtt accacatcta tgccgggttg ttcgtcagca tgagtgttgt
    17161 tgatgagaat aggtaattta tagtcgaagg ttccagcttg caactgtttc agcatctggt
    17221 cgggttgggt cagcatgagt ggtgttgtct cagtaagata tttatcaata cttgcttgat
    17281 ctttagccac aacagccatt ttaatgtctc taccttcagc tgattgacca attgactcaa
    17341 tccttactag gcggtcggtc gcagcattat ttttcgtttc ctcaatctct gtcaacattt
    17401 cctcatgtgt acggtaggct tcataaggac ggaaatgaag agtttttgaa attatcaagt
    17461 ttccatcggc actagtacct tccaacagca tattttttat aaaatgtcga taggtacggc
    17521 gaatattact cggtgtccgt ttactcaaat cttctccaaa gagagctgcg agctggatat
    17581 tcaaatgaag ttggttatcc tgtctgcttt cttctactgt gataaaagta tctcctgtga
    17641 aatctccctc tttcaaattc caggttttcc attcggaaat agactttcca tccaatgtcc
    17701 attctatttt ttcagtcgta tctggaagtg tgatggattc tgtgatagtg actgcttccg
    17761 atagatagac agtttttgtt tcgagtgtaa ctgtatcaat agtcggtacg gcagattttt
    17821 cttctgtagt atcctgatta tcattattag ctgttctgag cgtagtttcc tcggcattgg
    17881 gctcggaaga tgttgtatct tcttctgtgg aaacaggtgt gggagaagta tctgctattt
    17941 cttcagtttg tttttctgtc tccttccctt ctacaagatt atctgtagct tcgcttgtgc
    18001 tagttttcgt aataggagtc tcttcaggca gtggagtcac tgtttcacta gtagtaggtg
    18061 gtatctcctc tgtatttgcg aagaccgttc cctgagcgag agatgcagct aaaagaactg
    18121 ttgaggcaag taggacttga ttcaacagcc ttttcttata tgaaagtttc atgcgaactt
    18181 cctccttttg ataatgtgta cggggtaata aattaaaacg ggttatttca tgcctatctt
    18241 atcaaa
//
LOCUS       DBNFCK010000030        17522 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.030, whole genome shotgun sequence.
ACCESSION   DBNFCK010000030 DBNFCK010000000
VERSION     DBNFCK010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 17522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17522
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.030"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            116..565
                     /locus_tag="AC1PIW_000801"
     CDS             116..565
                     /locus_tag="AC1PIW_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIP6605757.1"
                     /translation="MGHTIADFRNLLNQIEQISETIAKEYDVEHLAGPQGWALRFIAE
                     RSEAETFVKDIEAELKISKSVASNLVKRMEKNGFIQVLPSKVDKRFKQLVLTEKGQGK
                     ICHLKAFHEEMHHSLFWGIQKEDFDLVKQVGNQLKVNIQRYKEKNHV"
     gene            558..2264
                     /locus_tag="AC1PIW_000802"
     CDS             558..2264
                     /locus_tag="AC1PIW_000802"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6605758.1"
                     /translation="MFKEAILRYKWYALASVLLTSIVVATTLMQPSYLQDVLTAVLAN
                     DKDEIVRVGKLLLIIAGIGLLAGLVNTIAAAKISQGVSADIREKTFRKIQSFSYANIE
                     EFNAGNLVVRMTNDVNQIQNLVMMLFTVLMRVPLLFVGAFIMAVRTMPELWWMIILMV
                     VLIMAIMAVVMGQMGPRFGKFQSLMDKMNSIAKENLRGIRVVKSFVQEKNQYAKFKKV
                     SNELLDLNLFIGYGFAILQPLMMFISYMAIFASLYLVSGMLETNLEAVGGFTSFMSYL
                     MQIMFAIIMTSFMGMQASRAAISIKRISEVLDTEPAMTFKDVADEELTGKIVFDNVSF
                     TYPHDTEPTLKNISFEIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQEGTVSIGGRDL
                     KEVSKNTLRDTVSIVLQKAILFSGTIADNLRQGAPGADLQRLERAAGIAQAKEFIDRL
                     DDTYESQVEERGNNFSGGQKQRMSIARGVIGEPKVLVLDDSTSALDAKSEKLVQEALN
                     HDLKGTTTVIVAQKISSVVKADKILVLDEGRLIGQGTHAELVATNDVYREIYETQKGR
                     EE"
     gene            2265..4049
                     /locus_tag="AC1PIW_000803"
     CDS             2265..4049
                     /locus_tag="AC1PIW_000803"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6605759.1"
                     /translation="MKTLRFFWFYFKRYKLSFAVIFLAIVAATYLQVKTPVFLGNAIA
                     EMGKIGQAYFMANQAGQADFQPDMADFNGVMLNLFFAYAATVVASLIYTLLFTRIVAH
                     STNRMRKGLFGKLERLTVAFFDSHKDGDILSRFTSDLDNIQNAFNQSLTQVVTNIALY
                     VGMVIMMFRQDTRLALVTIASTPVALIALVVIIRLSRKYTDKQQAAVSKLNAYMDEKI
                     SGQKAIIVQGVQEETIDGFLELNEEVRRTTFKGRLFGGILFPFMNGMSLVNTAIVIFA
                     GSSIVLNDSSLETAAALGLVVTFVQYSQQYYQPIMQVAASWAELQLAFTGAHRIQEMF
                     DEPEEVRPQNAPLFTELKEGVEIKDIDFGYLPGQKVLDKVSISAPKGKMVAVVGPTGS
                     GKTTIMNLINRFYDVNGGSVAFDGRDIREYDLDSLRNKVGIVLQESVLFSGTIADNIR
                     FGDESISQEMVETAARATHIHDFIMSLPEGYETFVTDDENVFSTGQKQLISIARTLLT
                     DPQVLILDEATSNVDTVTEAKIQKAMEAIIAGRTSFVIAHRLKTILNADEIIVLKDGK
                     VIEQGNHSQLLKLNGFYAELYHNQFVFE"
     gene            4155..4598
                     /locus_tag="AC1PIW_000804"
     CDS             4155..4598
                     /locus_tag="AC1PIW_000804"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HIP6605760.1"
                     /translation="MKIRGFELVSQFTDENLLPKRETAHAAGYDLKAAETVSLEPGEI
                     KLVPTGVKAYMQANEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPANEGHIFAQM
                     RNITEETVVVEAGERIVQAVFAPFLLADGDQADGVRTGGFGSTGK"
     gene            4605..5138
                     /locus_tag="AC1PIW_000805"
     CDS             4605..5138
                     /locus_tag="AC1PIW_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIP6605761.1"
                     /translation="MQILFVRHSEPDYSMFDQHDNPRLYAGFGRDLAPLTEKGRTLAQ
                     EIASNPIFSQAQVVVASSVTRALETATYIAHAQQLPLMVEPFFHEWRPDMTGQNASQD
                     EAVLAHRLFLENSGAVPESSPVRYETAAEMRERFLQALGKYKAYDRIVIVCHGMLIRQ
                     FVPKETIAYCEILEYTL"
     gene            5145..6512
                     /gene="radA"
                     /locus_tag="AC1PIW_000806"
     CDS             5145..6512
                     /gene="radA"
                     /locus_tag="AC1PIW_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIP6605762.1"
                     /translation="MTIAKKKTTFVCQSCEYHSPKYLGRCPNCGSWSSFVEEVEVAEV
                     KNERVSLTGEKTRPMKLNEVSSIQVARTKTNMEEFNRVLGGGVVPGSLVLIGGDPGIG
                     KSTLLLQVSTQLSTIGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSIRTE
                     IEKIKPDFLIIDSIQTIMSPDISSVQGSVSQVREVTNELMQIAKTNNIATFIVGHMTK
                     EGTLAGPRTLEHMVDTVLYFEGERQHTFRILRAVKNRFGSTNEIGIFEMQSQGLVEVL
                     NPSEVFLEERLDGATGSAIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRAS
                     LIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVALASSYKDKPTNPQECFM
                     GEIGLTGEIRRVNRIEQRINEAAKLGFTKVYAPKNSLTGIKVPKEITVIGVTTIGEVL
                     QKVFN"
     gene            6636..7130
                     /locus_tag="AC1PIW_000807"
     CDS             6636..7130
                     /locus_tag="AC1PIW_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HIP6605763.1"
                     /translation="MSYFQNFMKANKAYVDLHGDYHLPLRPKTKVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQLGTWEIVVLHHTDCGAQTFTNEEF
                     AVQLKRDLGVDVAGQDFLPFTDIEESVREDIALLKQSPLIPDDVEISGAVYDVDTGRI
                     TEVI"
     gene            7338..8096
                     /locus_tag="AC1PIW_000808"
     CDS             7338..8096
                     /locus_tag="AC1PIW_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HIP6605764.1"
                     /translation="MSDNRMKYTIDSNMQFPLVEIALEAGESAYIQQGSMVYHTPSVT
                     LNTKLNARGGSGFGKLMGAIGRSMVSGESMFITQAISNSDDGKIALAPSTPGQVIALE
                     LGAEQYRLNDGAFLALDGSAQYKMERQSVGKAFFGGQGGLFVMTTEGHGTLLANAFGS
                     IKKLTLDGGSITIDNAHVVAWSRDLDYNIHMENGFLQSIGTGEGIVNTFTGYGEIYVQ
                     SLNIETFAQVIGNHIVGAGGDGGGGSTSLLDAIF"
     gene            8274..8720
                     /locus_tag="AC1PIW_000809"
     CDS             8274..8720
                     /locus_tag="AC1PIW_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HIP6605765.1"
                     /translation="MKVRIEFDDSLDQVEVVIRTSQLGPEIEQIQQALQQVSRPSLVF
                     YKGSSEYFLSLGDILFFETDGTKIFAHTGDDAYEVKMKLYELEEHLPIYFCRVAKSTI
                     VNSKLVYSLNKSFSGTSRISFYKTHKEVHVSRHYYHLLKEKLQEMR"
     gene            8725..9384
                     /locus_tag="AC1PIW_000810"
     CDS             8725..9384
                     /locus_tag="AC1PIW_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF transmembrane domain-containing protein"
                     /protein_id="HIP6605766.1"
                     /translation="MKRLLIGAAVIGLAVLVLLRESLGLHLAMPFWTTVWFVLTFVTG
                     FHRLVKLDWLASIFCFGLAFIAANSVYEWVEFGTWNMFLSLVLLCLGLKLIFKPRKRR
                     LAHGVVTGVIGDKGRDTAFGSSTRYIHEENFIHDSVDVAFGSSTVYFDQAVILGNKAD
                     FHIDAAFSNITLYLPADWQAVVELDSAMSTTNNYASEPKEKILLVTGDMAFSTLNIYS
                     M"
     gene            9476..10756
                     /locus_tag="AC1PIW_000811"
     CDS             9476..10756
                     /locus_tag="AC1PIW_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIP6605767.1"
                     /translation="MKKILKNATYLWVLTADMLSNFGDVVYYLALMNYVLLVPNSRLA
                     LAIVTFSEIFPSFMGLFTGYLADKTVNKISTIKLTLLFRVLLYLILGFCMGFEPALWI
                     VVVAASFNVFADFAGFYENGLYTPLGLRVAPKEEREQYSAFRQTVTSLLNIAFQALSA
                     LLVGLLSYQNLAFLNAGTFLASLLIMQLLTPAFRKLLTEQPLKIAEQPNQTEPKTKGP
                     GILASFKQAIVELRKIPEFRLVLITNPLINACGAILYPILVLLISEDPGLVFLNVETT
                     LALTILIFFIGHILGSTLVFTLFKNTSMVTLEVAATFSLLGVFVGMLLHQLPIIFFFL
                     TTMGISSGASGPKFNAKFVNSMPEEQLATIGGAVSTYFMLGQAFTRLLVSGLVLILTV
                     NQISGLFLAATGLLVLYVFYWLARNQKTPQNQSV"
     gene            10828..12282
                     /gene="gltX"
                     /locus_tag="AC1PIW_000812"
     CDS             10828..12282
                     /gene="gltX"
                     /locus_tag="AC1PIW_000812"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIP6605768.1"
                     /translation="MTKPIRVRYAPSPTGLLHIGNARTALFNYLFARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGIDWDESPETHENYRQSERLDIYQKYVDQLLAEGKA
                     YKSYVTEEELAAERERQEAAGETPRYINEYLGMSEDEKTAYIAEREAAGIVPTVRLAV
                     NEAGIYKWNDIVKGEIEFEGGNIGGDWVIQKRDGYPTYNFAVVIDDYLMKISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRRKGYM
                     PEAVFNFIGLLGWNPGGEEEIFSREQFIQLFDENRLSKSPAAFDQKKMDWMSNEYIKN
                     ADLETIFNLAKPFLEEAGRLTDKAEKLVELYKPQMSSADEIVGLTDLFFSDFPELTAE
                     EKEVMAGETVPTVLNALKEKLEAMTDEDFQPDNIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPNTIYLLGREKSIQHIDNMLKSL"
     gene            12419..13795
                     /locus_tag="AC1PIW_000813"
     CDS             12419..13795
                     /locus_tag="AC1PIW_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIP6605769.1"
                     /translation="MENHNFENQGTFNREMNSRHLQMLSIGGVIGTGLFLSSGYTIAQ
                     AGPFGAVAAYLFGAVMVYLVMFSLGELSVAMPVTGSFHTYATKFISPGTGFMVAWMYW
                     LCWVVALASQFVGAAQLMQRWFPSVPIWIFATIFAVIVFGLNTLSVGWFAKAEDALSS
                     IKVYAIAAFIVLGTLAIFGILPFEGTNAAPLFTNITAQGLLPNGLVGLISVMLSVNYA
                     FSGVEMIGIAAGETDNPKKAVPQAIKSTIGLLVIFFVLTIVVLAALLPMSEAGVTEAP
                     FVLVLDKIGFPYAADIMNFIILTAILSASTSGLYASSRMLWSLANEGMISKELVKINK
                     HGVPMRGMILSMIGVVIALVASIYAEDTIFLALVSIAGFAVVIAWLAIPLAQIGFRRE
                     FLKNHSEDELEYKTPFSPTLPWITVVLLVISIIGIGWDPSQRAGLYFGVPFMIGCYIY
                     HYIRFKKW"
     gene            13808..14755
                     /gene="mmuM"
                     /locus_tag="AC1PIW_000814"
     CDS             13808..14755
                     /gene="mmuM"
                     /locus_tag="AC1PIW_000814"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047150 - betaine-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HIP6605770.1"
                     /translation="MGRFKELLEQKEYIILHGALGTELEFRGHDVSGKLWSAKYLLEN
                     PQYIKDIHKDYIRAGADLVTTSTYQATFEGLAEVGLSQAEAEELIRLTVDLAKEARDE
                     VWAELSEAEKVQRTYPLISGDVGPYAAYLANGAEYTGDYGNISLSELKDFHRRRIELL
                     LEQEAELLALETIPNVLEAQALVELLAEDFPEAEAYISFTSQDGQSISDGTSIEKIAE
                     LVNSSEQILAVGLNCTAPSLYPAFLSQLREKTDKPFVTYPNSGEVYDGATQTWKEKAD
                     DSHSLLDNTLEWHELGAKVVGGCCRTRPADIADLVAGLK"
     gene            complement(14805..15305)
                     /locus_tag="AC1PIW_000815"
     CDS             complement(14805..15305)
                     /locus_tag="AC1PIW_000815"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIP6605771.1"
                     /translation="MEIWNAYTADGHLTDHTLTRGESIPDGLYHLVVECIIRHHDGST
                     LFMKRDSTKPSYPDYYEATAGGSALFGEIAEQAILREVREETGIELTADQLRHHKHFV
                     AHDDQCIFHCYWAETDWDKVAIQLQANETSNYIWVQQENLKDFLETELVIPRQKDYVE
                     RLFLES"
     gene            15412..16287
                     /locus_tag="AC1PIW_000816"
     CDS             15412..16287
                     /locus_tag="AC1PIW_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIP6605772.1"
                     /translation="MQPIIPHLWYDTEAKEAVAFYVDLFGGNVDWTYTITDTPSGDSD
                     LIQFQLGDMTLAAISAGPYFTLNESMSLMVNVADKDEVTRLYQALSEGGRILMPLGEY
                     PFSPYYVWLEDRFGLSWQLSYAPDLDKPYQFDICLLFSQEQVGLAQPMLDYYKDKLPQ
                     ASVGQLSYYGEGEAAVEAAKLNYAELLVAGQKMIVMDHGYGGVASFNEAFSFMVYVDS
                     QEEAESWYEKVSAVPEAEICGWAKDQFGISWQIVPRILMEAYDTANPEKVKAVNAAVM
                     TMKRLDIAAIQALLN"
     gene            16393..16917
                     /locus_tag="AC1PIW_000817"
     CDS             16393..16917
                     /locus_tag="AC1PIW_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HIP6605773.1"
                     /translation="MKATERRKKILSLLRESPYPLAANYLAKEFGVSRQVIVGDVALL
                     RAEGEAVLATPRGYLLQSSLPSTEGAYIRKIACQHGPEEVEKELTILLEGGAQILDVE
                     IEHPIYGLLSGKLNIASLEDRDHFLDALGQYQGVLLSNLTEGVHTHTVSFPSVQAYNE
                     LSAALKHAGILLEN"
     gene            16990..>17522
                     /locus_tag="AC1PIW_000818"
     CDS             16990..>17522
                     /locus_tag="AC1PIW_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605774.1"
                     /translation="MSQLTLSSKNSVKQLSISAILTAFAILIPLMMPIKIIIGPASYT
                     LASHIPLFIAMFISPATAIFVALGSSLGFFLAGFPIVIVFRALTHLFFLTLGAVLVKR
                     FPILMDSKRFLLLGIGLNLLHGLGEYIVVMVLTSGQQTSATYWITMLGLVGVGSAIHG
                     LLDFSLAYYFWKILKER"
BASE COUNT         4516 a         3722 c         4289 g         4995 t
ORIGIN      
        1 atgatgctga aataacaaaa tattttcaga aaagagcaaa cgaaataatt gacaatagtt
       61 cttaagagaa ctataatagt tctcatggta attattttcg tagaaaggag ctgtcatggg
      121 acatactatt gcagattttc ggaacttgct caatcagatt gaacaaatta gtgaaaccat
      181 tgcaaaagaa tacgatgtgg agcacttggc tggtccacag ggctgggcct tgcgcttcat
      241 tgcggaacgg tcggaagccg aaacctttgt aaaagatata gaagcggaat taaagatttc
      301 caaatcggtt gccagcaatc tggtcaagag aatggagaaa aatggcttta tccaagtcct
      361 tccctctaag gttgacaaac gcttcaaaca gctggttttg acagagaagg gacaagggaa
      421 gatatgtcac ctgaaagctt ttcatgaaga aatgcaccat tcactttttt ggggcattca
      481 aaaagaggac tttgacttgg ttaaacaggt gggcaatcaa ttaaaagtaa atattcaacg
      541 ctataaggag aagaatcatg tttaaagaag ccattttacg ttataaatgg tacgccttgg
      601 catcggtctt gctgacgtca attgtcgtag caacgacctt gatgcaacct agttatttgc
      661 aggatgtttt gacagcagtc ttggccaatg acaaggatga aattgttcgt gtgggcaaat
      721 tactgctgat tattgctgga attggtcttt tggcagggct tgtcaatacc attgctgcag
      781 caaaaatttc acaaggggta tctgcagata ttcgtgagaa gacctttcgg aagatccaaa
      841 gcttctctta tgctaatatc gaagagttta acgcagggaa cctggttgtt cggatgacta
      901 acgatgtcaa tcaaatccag aaccttgtca tgatgctctt tacagtgttg atgcgggttc
      961 cattgctgtt tgtcggagcc tttatcatgg cagtgcggac catgccagaa ctctggtgga
     1021 tgattattct catggtggtc ttgattatgg ctattatggc agttgtcatg gggcaaatgg
     1081 gaccacgttt tgggaaattc cagtccctca tggacaagat gaacagtatt gctaaggaaa
     1141 acctccgtgg gattcgtgtg gttaagtctt ttgtacagga aaaaaatcag tatgccaagt
     1201 tcaagaaagt atccaatgaa ctcttggacc tcaacctctt tatcggctat ggttttgcta
     1261 tcttgcaacc cttgatgatg tttatctctt acatggcgat ttttgcatcg ctctacttgg
     1321 tgtccgggat gttggaaacc aacttggagg cagtcggtgg ctttacttcc tttatgagtt
     1381 acctcatgca aatcatgttt gccattatca tgactagctt catggggatg caggcttcac
     1441 gtgctgctat ttctattaag cggatcagcg aggtcttgga tacagaacct gctatgacct
     1501 tcaaggatgt ggcggatgaa gaattgactg gtaagattgt ttttgacaat gttagcttta
     1561 cctatccaca cgatacggag ccgactttga aaaatatctc atttgagatt gagccaggtc
     1621 aaatggtcgg tgtggttggt gcaacaggtg cgggtaaatc cactcttgct caactgattc
     1681 cgcgtttgtt tgacccgcaa gaaggaacgg tgtccatcgg tggtcgggac ttgaaagaag
     1741 tcagcaagaa caccctgcgt gatacggtat cgattgtctt gcaaaaggct attctcttct
     1801 cgggaaccat tgcggacaac ctgcgtcaag gggcacctgg tgctgatttg caacgcttgg
     1861 agcgggccgc aggtattgcc caagccaaag aatttatcga ccgcttggat gatacctatg
     1921 agagccaagt agaagaacgt ggaaataact tctctggtgg ccagaagcag cggatgtcca
     1981 ttgctcgtgg ggtgattggt gagcctaagg ttttggtctt ggatgactcg acttctgccc
     2041 tggatgccaa gtctgagaaa ctggtccaag aggctctcaa ccatgatttg aaggggacaa
     2101 ccactgtcat cgttgcccag aagatttctt ctgttgtcaa agctgataag attttggttc
     2161 ttgacgaagg ccgtctaatt ggacaaggaa ctcacgctga gttggtagcg acgaacgatg
     2221 tttaccgtga aatctacgaa acacagaaag ggagggagga atagatgaag acgttacgtt
     2281 ttttctggtt ttattttaaa cgctataaac tgtcctttgc tgtgattttt ctagccattg
     2341 tggcagcgac ctacctgcag gttaagacac ctgttttcct tggaaatgcc attgcggaga
     2401 tggggaaaat cgggcaggct tactttatgg ccaatcaagc tggtcaggct gactttcagc
     2461 cagacatggc tgattttaac ggggttatgc tcaatctttt ctttgcctat gcggcgacgg
     2521 ttgtggcttc cttgatttac actctcctct tcacgcgtat cgtggctcat tcgaccaacc
     2581 gtatgcgtaa gggcttgttt ggcaaactgg aacgcttgac agttgccttc tttgatagcc
     2641 acaaggacgg ggatatactt tctcgcttta ccagtgattt ggacaatatc caaaacgctt
     2701 tcaaccagtc cttgacccaa gtggtgacca acatcgctct ttatgttggt atggtcatca
     2761 tgatgttccg tcaggatact cgcttggcct tggtgaccat tgcttctacg ccagttgcct
     2821 tgattgcctt ggtcgttatc atccgcctat cacggaaata tacggataag caacaggctg
     2881 cggtgtctaa actcaatgcc tacatggacg agaaaatttc tggacaaaaa gcgattattg
     2941 tacaaggtgt gcaggaagag acaattgatg gtttcttgga gctcaatgaa gaagttcgtc
     3001 gcacaacttt caagggacgc ttgtttggtg ggattctctt cccatttatg aatggtatga
     3061 gtttggtcaa tacggccatt gttatctttg caggttccag cattgttctc aatgacagct
     3121 cactggaaac agctgccgca cttggtctgg tggtgacttt tgttcaatac tcgcaacagt
     3181 attaccagcc aatcatgcag gttgctgcca gctgggcaga attgcagcta gctttcacag
     3241 gagctcatcg tattcaggaa atgtttgatg agcctgagga agttcgtcct caaaacgctc
     3301 cgctatttac cgaattaaaa gaaggtgttg aaattaagga catcgacttt ggctacttgc
     3361 caggtcagaa ggtcttggac aaggtgtcta tctctgctcc taagggtaag atggtggcgg
     3421 tcgttggtcc gacgggatct ggtaagacta ccattatgaa cttgattaac cgcttctacg
     3481 atgtcaatgg tggtagtgtg gcctttgatg gtcgcgatat tcgggaatat gatttggata
     3541 gcttgcggaa taaggtcggt atcgtcttgc aggagtcggt gttattctcg ggtactattg
     3601 cggacaatat tcgctttggt gatgagagca tttcgcagga aatggtggaa accgcagctc
     3661 gtgccaccca tatccacgac ttcatcatga gcttgccaga gggctatgaa acctttgtga
     3721 ccgatgatga gaatgtcttc tcaacaggtc agaaacagtt gatttccatt gcccgtacgc
     3781 ttttgacaga cccacaagtc ttgattttgg acgaagcaac ctcaaacgtt gataccgtaa
     3841 cggaggccaa aattcaaaag gctatggagg ccattatcgc aggacggact agcttcgtca
     3901 ttgcccaccg cctcaaaacc attctcaatg cggatgaaat catcgtcctc aaggatggaa
     3961 aggttatcga gcaaggcaac cacagccaac ttctcaaact aaatggcttc tacgccgaac
     4021 tttaccacaa ccagtttgtg tttgaataaa gttgaaaacc ccagccacta ggttggagtt
     4081 ttgcttttag cctgcgaata aattataata gagagcgtat gtattatgca tgaagaaatt
     4141 gaagggaaac tactatgaaa atccgtggat tcgaactggt cagccagttt actgatgaaa
     4201 acttattgcc aaaacgtgag accgctcatg cagcaggtta tgacctgaaa gctgcagaaa
     4261 ctgtcagctt ggagccaggc gagattaagc tggtgccgac aggtgtaaag gcctatatgc
     4321 aggcaaacga agtcctctat ctctacgacc gctcgtccaa tcctcgcaag aagggtctgg
     4381 tcctgattaa ctcggttggg gttatcgacg gggactacta cggcaatcca gccaacgaag
     4441 gccacatctt tgcccagatg cgaaacatca cagaggaaac cgtcgtggtg gaagctggtg
     4501 agcggattgt gcaggctgtt tttgccccct tcctcttggc tgacggtgat caggccgacg
     4561 gcgttcggac gggcggattt ggatcgacag ggaaatagac tactatgcag attctctttg
     4621 tgcggcattc cgaacccgac tacagtatgt ttgaccagca tgataatcct cggctgtatg
     4681 caggttttgg acgggatttg gctcctttaa ctgaaaaggg gcggacttta gctcaggaaa
     4741 ttgctagtaa tcctatcttt tcccaagctc aagtagtcgt cgcttcaagc gtcacacggg
     4801 ccttggaaac tgcgacctac attgctcatg cccagcaact acctcttatg gtcgagccct
     4861 tttttcatga atggcgacca gatatgacgg ggcaaaatgc cagtcaagac gaggctgtct
     4921 tagctcatcg attatttttg gaaaatagcg gagcggtgcc tgagtcttca cctgttcgct
     4981 atgagactgc tgcggagatg agagagcgat ttttacaggc ccttggaaaa tacaaagcct
     5041 atgaccgaat cgttatcgtc tgtcacggta tgctgatacg ccagtttgtt cccaaagaaa
     5101 ctatagccta ctgcgaaatc ctagaataca ctctatagga ggaaatgacc atcgctaaga
     5161 aaaaaacaac ctttgtctgt caatcctgcg agtaccactc gcccaagtac ctgggtcgtt
     5221 gccccaactg tggctcctgg tctagctttg tcgaggaagt ggaagtcgct gaagtcaaga
     5281 acgagcgggt cagcctgaca ggtgagaaga cccgtcccat gaaactcaat gaagtgtcat
     5341 ccattcaagt ggctcgcact aagactaaca tggaggagtt caaccgcgtc ctcggtggcg
     5401 gtgtggtacc ggggagtcta gttctcattg gcggcgatcc agggattggc aagtccacct
     5461 tgctcctgca agtatccact cagctgtcta ccattggcac cgtcctctat gtgtcggggg
     5521 aagagtctgc ccagcagatt aagttgcgag cggaacgctt gggcgacatt gacagcgagt
     5581 tctatctcta tgcagagacc aatatgcaga gcattcggac cgagattgag aaaatcaagc
     5641 cagatttcct gattatcgac tctatccaga ccattatgag tcctgacatc tccagcgtgc
     5701 aaggctctgt cagtcaggta cgtgaagtga ccaatgagct catgcagatt gccaagacca
     5761 acaatatcgc aacctttatc gtcggccaca tgaccaagga aggaaccttg gctggaccgc
     5821 ggaccttgga gcacatggta gacaccgttc tctattttga aggcgagcgg cagcacacct
     5881 tccgtatctt gcgggcggtc aaaaatcgct tcggctccac caatgaaatc ggcatttttg
     5941 aaatgcagtc gcagggcttg gtcgaagtcc tcaatccaag tgaggtcttt ctggaagagc
     6001 gtctggacgg agcgactggc tcggctattg tcgtgaccat ggagggtacc cgcccaatcc
     6061 ttgcggaagt gcaggctctg gtgacgccga ctatgtttgg caatgccaag cggaccacga
     6121 caggactgga tttcaaccgt gctagcttga ttatggcggt tttggaaaaa cgggcaggcc
     6181 tgctgctcca aaaccaagac gcctacctca aatcagcagg cggtgtcaaa ctggatgagc
     6241 cagctattga cctggcagtc gcagttgccc ttgcttccag ttacaaggac aagccaacca
     6301 acccacaaga gtgctttatg ggcgaaatcg gtctgacagg ggaaatccgc cgcgtcaatc
     6361 gtattgaaca acggattaac gaagccgcca aattgggctt taccaaggtc tatgccccca
     6421 aaaattccct gacaggtatc aaggtgccca aggaaatcac cgttatcggc gtgaccacca
     6481 ttggcgaagt cttgcagaaa gtattcaact gatgaaatca ttcgggagtt acagctagtc
     6541 gctcctcttt ttgcgacaaa tagtcgggaa tgtgataagc taggtctaac ctatttctga
     6601 aaatttcaga aattctcatc agacaaggag gtcctatgtc ttattttcaa aactttatga
     6661 aggccaacaa ggcctatgtt gacctgcatg gtgattacca tttgcctctt cgtccaaaga
     6721 ccaaggtggc tatcgtgacc tgtatggact cacggcttca cgtggcgcag gccttaggct
     6781 tggctctggg ggatgctcac attctgcgga atgcgggtgg gcgcgtgaca gaagacatga
     6841 ttcgctcgct agtcatttcc cagcagcagc tcggtacatg ggaaatcgtt gtcctccacc
     6901 atacagactg cggtgcacag acctttacca atgaagaatt tgcagtccaa ctcaagcgag
     6961 atttgggagt ggatgtggct ggtcaggact ttcttccttt tacagacatt gaagaaagcg
     7021 tgcgggagga tattgctctc ttgaaacaat ctccgctcat acctgacgac gttgaaattt
     7081 ctggtgcggt ttatgatgtg gatacgggtc gtataacaga agttatatag gaatgctcag
     7141 gaagttcggt ttttcctgta aattttatct gaatgaccgg gaaatccttg acaagttttt
     7201 ttgaatgaga taaaattgta ttatcacaac agaacaaatg aagagataaa tgatacttgg
     7261 tttattttag cttgttctag gtttattatg gctatcctgt ggttaacagg acctttcgta
     7321 aaaaatagga gtcaactatg tcagacaatc ggatgaaata tactatcgat agtaatatgc
     7381 agtttccttt ggtggaaatc gccttggaag ctggtgaatc agcttatatt caacagggga
     7441 gcatggtcta ccatacgccg agtgtgacct tgaataccaa gctcaatgcg cgtggtggtt
     7501 caggttttgg taaattgatg ggggccatcg gtcggtcaat ggtatcaggc gaatctatgt
     7561 ttatcacgca agccatttcc aatagtgacg atggaaaaat tgcacttgcg ccttcaacgc
     7621 caggtcaggt tattgccttg gaacttggtg cagagcaata tcgactaaat gatggtgcct
     7681 tccttgccct tgacggttct gcccagtata aaatggaacg ccagagtgtt gggaaagctt
     7741 tctttggtgg tcagggtgga ctctttgtga tgactacaga gggccatggc acacttttgg
     7801 cgaatgcgtt tggttctatt aagaagctaa ctttggatgg tggaagtatc accattgaca
     7861 atgctcatgt ggttgcttgg agtcgtgatt tggactacaa tatccatatg gaaaatggtt
     7921 tcctccaatc tatcggtaca ggtgaaggaa tcgttaatac ctttactggt tatggagaaa
     7981 tctatgtaca gagtttgaac attgagacct ttgctcaggt cattggcaat catattgtcg
     8041 gtgcaggtgg tgatggtggt ggtggaagca catcgttgct ggatgcgatt ttttaagaag
     8101 aaaaactctg caattttcaa aatcatataa taaaaataaa gtccctcgcg taatgcaagg
     8161 gacttttctt agtttatttc taccacttac ctgtctaatt ttgccactta ctgaaatgcc
     8221 cttgtaattg gatgtcgaac tggtataata gagctatcaa aaaggaggga gctatgaaag
     8281 ttaggattga atttgatgac agcttagatc aggttgaagt tgttatccga accagccagc
     8341 tgggaccaga gattgaacag attcagcaag ctttgcaaca agttagtcgt ccctcgcttg
     8401 ttttttacaa ggggagcagc gagtattttc tttcattggg ggacatactt ttctttgaaa
     8461 cggatggaac caagattttt gctcatacag gcgatgatgc ctacgaggtt aagatgaaac
     8521 tctatgaact tgaggagcat ttgcccatct acttttgtag ggtagctaag tcaaccattg
     8581 tcaatagcaa gttagtctat tcgctaaata agtccttctc gggcaccagt cggatttcct
     8641 tctataagac tcacaaggaa gtccacgtgt cgcggcatta ttatcattta ttaaaagaaa
     8701 agttacagga aatgaggtag gaagatgaaa cgtttattaa ttggagctgc agttatagga
     8761 ctggcggttt tggtcttgtt gagggagtca ttgggtttgc atttggcaat gcccttttgg
     8821 accactgtct ggtttgtatt aacctttgtg acaggttttc accgcttggt aaagttggat
     8881 tggcttgcca gtatcttttg tttcggccta gcttttatag ctgccaactc tgtctatgaa
     8941 tgggtggagt ttgggacttg gaacatgttt ctgtcgcttg tcttactctg tcttggcttg
     9001 aaactaattt ttaaaccaag gaagcgcaga ctggctcatg gagttgtcac aggggtgatt
     9061 ggcgataagg gacgcgatac agcttttggt tcctcgactc gatacatcca tgaggaaaat
     9121 tttatccatg atagtgtgga tgtagccttt ggatctagta cagtctattt tgatcaggcg
     9181 gtgattctgg gtaataaggc agattttcat atcgatgcgg ccttctcaaa tatcacactc
     9241 tatctgcctg ctgactggca agcggttgta gagttggata gcgccatgtc aacaactaac
     9301 aactacgcat ctgagccaaa agaaaagatt ctcttggtga cgggtgatat ggcattttct
     9361 acattaaata tttactctat gtaaaagaat aatcgcagag gaaggtctat ccaggctcct
     9421 ctgtaggaca aaaaatcaga atattctgaa aattaatcaa aagaaaggga ttatcatgaa
     9481 aaagattttg aaaaatgcta cttacctatg ggtactgact gcggatatgt tgtccaattt
     9541 tggcgatgtg gtctattatc tggcattgat gaactatgtt ttactggtgc ccaacagtcg
     9601 tctggctctg gcgatcgtga ccttctcgga aatcttccct agctttatgg ggctctttac
     9661 gggctatcta gcagacaaga cggtcaataa aatcagcacc atcaagctga ccttactctt
     9721 tcgtgtcctt ctctacctca tcctcggatt ctgcatgggc tttgagccag ccctctggat
     9781 tgtcgtagtg gcagctagct tcaatgtctt tgcagacttc gctggcttct atgaaaacgg
     9841 cctctataca cctctgggat tacgcgtagc gcccaaagaa gagcgggagc agtactcggc
     9901 ctttcgccag accgtgacca gcctcctcaa catcgccttt caggccctct ctgccctctt
     9961 ggtcggcctc ctctcctacc aaaacctggc ctttctcaac gccggcacct ttttagcctc
    10021 ccttctcatc atgcagctgc tgacgccagc ctttcgcaaa ctcctgacgg agcagccttt
    10081 gaaaatagcg gagcagccga accagactga gccaaaaacc aagggaccag gcattctagc
    10141 ttctttcaag caagcaatcg tggagctgcg caagataccc gagttccgcc tggtcctgat
    10201 taccaaccct cttatcaacg cctgtggagc gattctctat cctattctgg tcctgctgat
    10261 tagcgaagac ccaggcctcg tcttcctcaa tgttgaaaca acccttgccc tgaccattct
    10321 gatcttcttt atcggacata ttctgggctc gaccttagtc tttacccttt tcaaaaatac
    10381 cagcatggtg acgctggaag tggcagccac tttctctctt cttggcgttt ttgtcggaat
    10441 gttgcttcat caactgccaa ttattttctt cttccttact acaatgggaa ttagttctgg
    10501 agctagtgga cctaagttta acgctaagtt tgtcaattct atgccagagg agcagttggc
    10561 gaccattggc ggagcggtat caacctattt tatgctaggt caagccttta ctcgactgtt
    10621 ggtttcgggt ttggtcttaa tcttgactgt caatcagatt agcggacttt tcctagctgc
    10681 aactggtttg ttagtcctct atgtcttcta ctggctggca cgtaaccaaa aaacacctca
    10741 aaatcagtct gtttagactg atttttctgt tttcttatgc atatctatgc tataatgttc
    10801 ttgattgaaa ttttacagga gagaaacatg actaaaccaa ttcgtgtgcg ttacgcacca
    10861 agtccaacgg gacttttgca tatcggaaat gcccgtacag cattgtttaa ctatcttttt
    10921 gcccgtcatc atggcggtac tttcctcatt cgtatcgagg atacagaccg caagcgccat
    10981 gtggaagacg gcgagcgttc tcaacttgaa aacctgcgtt ggttgggtat tgactgggat
    11041 gagagtccag aaacccatga gaactaccgt cagtctgagc gtttggatat ttaccaaaag
    11101 tatgttgatc agctcttggc agaaggcaag gcctacaagt cttatgtgac cgaagaagag
    11161 ttggcggcgg agcgtgagcg tcaggaagct gcgggcgaaa cacctcgcta catcaacgaa
    11221 taccttggta tgtctgagga tgaaaaaact gcctacatcg cagagcgtga ggcggctggc
    11281 attgtcccaa cggtccgctt ggctgtcaac gaagctggta tctacaagtg gaacgacata
    11341 gttaagggtg agatcgagtt tgaaggtggc aacatcggtg gtgactgggt tatccagaaa
    11401 cgtgacggct acccaaccta caactttgcc gtggtcattg atgattatct catgaaaatc
    11461 tcgcacgtta tccgtgggga tgaccacatt gccaacacac ccaagcagct catggtctat
    11521 gaagcacttg gttgggaagc gccagaattt ggtcacatga cgcttattat caattctgaa
    11581 acaggtaaaa agttgtctaa acgtgatact aacacccttc agttcatcga agattaccgc
    11641 cgtaaaggtt acatgcctga ggctgtcttc aactttatcg gtcttttggg ttggaacccg
    11701 ggtggtgaag aggaaatctt ctctcgtgag caatttatcc aactcttcga tgagaaccgt
    11761 ctcagcaagt cacccgctgc ctttgaccag aagaaaatgg actggatgag caatgagtac
    11821 atcaaaaatg ctgatttaga aactattttc aaccttgcta agccattctt ggaagaagcg
    11881 gggcgtttga cagacaaggc agaaaaattg gtggaacttt acaagccaca gatgtcatct
    11941 gcggatgaaa tcgttggttt gacagacctt ttcttctctg atttcccaga attgactgcg
    12001 gaagaaaaag aagtcatggc aggcgaaacg gttccaactg ttctcaacgc cctcaaggaa
    12061 aaattggaag ccatgacgga cgaagatttc caaccagata acatcttccc acaaatcaag
    12121 gctgttcaaa aagaaacagg catcaagggc aaaaacctct tcatgccaat ccgtatcgct
    12181 gtttcaggcg aaatgcatgg acctgagttg ccaaatacta tttacctgct cggccgtgaa
    12241 aaatccatcc agcatattga caatatgttg aagagtttgt aatgaaataa tccccactgt
    12301 catatggcgg tggggatttg tcatagtttg aagctataac acctggattg aaaacgatat
    12361 aaccagtttt tgaaaatcca tggtatgata gaataatcac aagaatttag gagagtttat
    12421 ggaaaatcat aattttgaaa atcaagggac ctttaaccgc gagatgaaca gtcgtcattt
    12481 gcagatgttg tccatcggcg gggttatcgg gacaggattg tttttgagtt caggctatac
    12541 cattgcacag gctggtccat ttggagctgt tgcagcctat cttttcggag ctgttatggt
    12601 ctatttggtt atgttttctt tgggtgagct ttcggtagcc atgccagtca caggttcttt
    12661 tcatacctat gcgaccaagt ttatcagtcc tggaacagga tttatggtgg cctggatgta
    12721 ttggctttgt tgggtagtcg ctctagcttc tcagttcgtt ggagcagcac agcttatgca
    12781 acgctggttt cctagtgtac cgatttggat ttttgcgacg atttttgcag ttattgtctt
    12841 tgggctaaat accctttctg ttggttggtt tgccaaggcg gaggatgcac tctcgtctat
    12901 caaggtctat gccatagcag cctttattgt gcttgggact ttagctattt ttggtatttt
    12961 gccatttgag ggaaccaatg ctgcgccgct ctttactaat attacagccc aaggtttact
    13021 tcctaacggc ctagttgggt tgatttcagt tatgctgtcg gttaactatg ccttttcagg
    13081 tgtggaaatg atcggaattg ccgctggaga gactgacaat cctaagaaag ccgtgccaca
    13141 agccatcaag tcaacaatcg gtctcttggt catcttcttc gtcttgacca ttgtagtctt
    13201 ggcagcccta cttcctatgt cagaagcagg tgtgacagaa gcgccattcg tacttgtatt
    13261 ggataaaatc ggttttcctt acgctgcaga tattatgaac tttattatct tgacggctat
    13321 tttatcggcc tctacatcag ggctatacgc ttcaagccgt atgctttggt ctttggcaaa
    13381 tgaaggcatg attagcaagg aattggtaaa aattaataaa cacggtgttc caatgcgtgg
    13441 catgatcttg tccatgattg gtgttgtgat tgccttggtt gcgtcaattt atgcagaaga
    13501 tactatcttc cttgctctgg tttccattgc tggttttgca gttgtgattg cttggttggc
    13561 cattccattg gcacaaatcg gtttccgacg tgaatttttg aagaatcata gcgaagatga
    13621 attggaatac aagacaccct tctcaccaac cttgccgtgg attacggttg ttttgttggt
    13681 tatttccatc atcggaatcg gttgggatcc ttctcagcgt gccggtcttt actttggtgt
    13741 gccattcatg attggctgct atatttatca ctacatccgc ttcaagaagt ggtaaggaga
    13801 aactagtatg ggtcgtttta aagaactatt agaacaaaag gaatacatca ttttacacgg
    13861 tgctcttggg acggaattgg aatttcgagg tcatgatgta tcaggtaagc tctggtcagc
    13921 caagtatctt ttggagaatc cccagtatat caaggatatc cacaaggatt atattcgtgc
    13981 cggagcagac ttggtcacga cttcaaccta tcaggcaacc tttgaaggat tagcagaagt
    14041 tggtttgtca caagcagagg ctgaagaact cattcgcttg accgttgatt tggccaagga
    14101 agcgcgtgat gaggtttggg cagaattgtc tgaagctgaa aaagtacaac gaacctatcc
    14161 tctcatctca ggtgacgtgg gtccctacgc agcctatttg gcgaacggtg ctgaatatac
    14221 aggcgattat ggcaacatta gtctgtcaga attaaaagat ttccaccgtc gcagaattga
    14281 actcctctta gaacaggagg ctgaactctt agccttggaa accatcccaa atgttcttga
    14341 agcacaagcc ttagtagagt tgctggcaga ggattttcca gaggctgaag cctatattag
    14401 tttcacctca caagacggtc agtcgatttc tgacggaacg tctattgaaa aaatagcaga
    14461 gctggtcaac agcagtgagc agattttggc ggttggtctt aactgtacgg ctccctcgct
    14521 ctatccagcc tttctcagtc agttgagaga aaaaacggat aaaccatttg tcacctatcc
    14581 gaattctggg gaagtctatg atggagccac tcaaacttgg aaggaaaagg cagacgacag
    14641 ccattctctt ctggacaaca cacttgaatg gcacgaactg ggtgccaagg tagttggagg
    14701 ttgttgccga acccgaccag ccgatattgc tgacctagtt gcaggtttga aataaaagga
    14761 gccccgctgt cctgtgacgg cggggatttg tctgttaggg gaatttagct ttctaaaaac
    14821 agcctctcca catagtcttt ctgccttgga atgactagct ctgtttccag gaaatctttc
    14881 aaattttctt gttgcaccca gatataattg ctggtttcgt tggcttggag ttggatagca
    14941 actttgtccc agtcggtttc agcccagtag cagtggaaaa tgcactggtc gtcatgggca
    15001 acaaagtgtt tatggtggcg tagttggtca gctgtcagct caatgcctgt ctcttcgcgg
    15061 acttcgcgca aaatggcctg ctctgctatt tctccaaaca aagccgaacc tcctgctgtc
    15121 gcttcataat agtcagggta ggagggtttg gtgctgtcgc gtttcatgaa gagggtgctg
    15181 ccgtcgtggt ggcggatgat gcactcgacg actaggtggt agaggccgtc gggaatggat
    15241 tctccgcgtg tgagggtgtg gtcggtcaag tgaccgtcgg ctgtgtaggc attccagatt
    15301 tccatagctt gctccttttc tatccttcta ttatagcata ggaagttttc ctgtgagctt
    15361 gtacggacaa gtgtgtggta taatgggcag acgaaagaaa aggagaaatt tatgcaacct
    15421 attattcctc atctctggta tgatacagag gcaaaagaag cggtggcttt ttatgtggac
    15481 ctctttggtg gcaatgttga ctggacctac acgattacgg atacgccttc tggtgattcg
    15541 gatttgattc agtttcaact gggggatatg accttggcgg ctattagtgc gggtccatac
    15601 tttacgctca atgagtccat gtcccttatg gtcaatgtag ccgacaagga tgaagtgaca
    15661 cgtttgtacc aagccttgtc agaaggcggt cgtattctca tgcctttggg agaatatcct
    15721 ttcagccctt actatgtctg gttggaagac cgctttggtc tgtcttggca actgtcttat
    15781 gcaccggact tggacaagcc ttatcagttt gacatctgcc tgctcttctc gcaggaacag
    15841 gtcggtttgg cccagcctat gttggactac tacaaggaca agctccctca ggctagtgtc
    15901 ggtcaacttt cctactacgg tgaaggggag gctgctgttg aggctgccaa actgaattat
    15961 gcggaacttc ttgtcgctgg acagaaaatg attgttatgg accacggcta cggtggcgtg
    16021 gcttctttca acgaagcctt ctcattcatg gtctatgtag acagtcagga agaagcggag
    16081 agctggtatg aaaaagtgtc ggcagttcca gaagcggaga tttgcggttg ggccaaggac
    16141 cagtttggga tttcttggca gatcgtgccg cgtattttga tggaagcata cgatacggct
    16201 aacccagaaa aggtcaaggc agtcaatgcg gctgtgatga ccatgaaacg tttggacatt
    16261 gcggctattc aagcattgct aaactagcag atgataacgg agactgttct gcagtttccg
    16321 tttttgggtt gaggaaaggt tgatttacag gaattttgat ataatggaaa aaagtgtacg
    16381 aaaggaatgt gtatgaaagc aacagaacga cgtaagaaaa tcctatccct tttacgagaa
    16441 agcccctatc cccttgcagc taattatctg gcgaaagaat ttggagtgag tcgacaagta
    16501 attgttggag atgtagcctt gcttcgtgcg gagggagaag ctgtcttggc aactccgaga
    16561 gggtacttac tccaatcttc tttgccatct actgaaggag cctacattcg aaagatagct
    16621 tgtcaacatg gtccagaaga ggtagaaaaa gaattgacaa tcttgctaga gggtggagct
    16681 caaatactag atgttgaaat tgagcatccc atttacggct tattatcagg caagctgaat
    16741 attgcaagtt tagaagatag ggatcatttt ttggatgcct taggtcaata ccaaggtgtg
    16801 ttactgagta atttaacaga aggagttcat acacatacag tctctttccc atctgtgcaa
    16861 gcctataatg aactttcagc agctttaaaa catgcaggta ttttactaga aaattaataa
    16921 atcttacagg cgtcttgaca cctgtaaggt tttgttttat aatcaaacca atagaaagag
    16981 aggtgcctaa tgtcccaatt aacactatca tctaaaaatt cagttaagca gctatcaatt
    17041 agtgcaattt taaccgcatt tgccatttta atacctttaa tgatgccgat taaaatcatt
    17101 attggcccag catcctatac acttgctagt cacattcctt tgtttatagc catgtttatt
    17161 tcaccagcta ctgctatttt tgttgcttta ggttctagtt taggcttttt cctcgccggt
    17221 tttccaatcg ttattgtatt tcgggcgctt acgcacctgt tcttccttac cttaggtgcg
    17281 gtcttggtca agcgttttcc gattctcatg gattctaagc gctttttact attaggaatt
    17341 gggcttaatc tcctgcatgg tctaggagaa tatattgtgg tgatggtgct tacttcgggg
    17401 caacaaactt ctgctaccta ctggataacg atgcttggct tggtgggtgt tggctcggct
    17461 attcatggat tgcttgattt tagtttagct tattatttct ggaaaatttt aaaagaacgg
    17521 aa
//
LOCUS       DBNFCK010000031        16990 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.031, whole genome shotgun sequence.
ACCESSION   DBNFCK010000031 DBNFCK010000000
VERSION     DBNFCK010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 16990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16990
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.031"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            31..951
                     /gene="coaA"
                     /locus_tag="AC1PIW_000819"
     CDS             31..951
                     /gene="coaA"
                     /locus_tag="AC1PIW_000819"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009568566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HIP6605775.1"
                     /translation="MKNEFLNFEQIDRATWQQLHRKTTIPLSQSELNSIKSFNDRIQL
                     HEVSDIYLPLVNLIHIYRKARKDLNFTKSLFLQKTIKPQPFIIGVSGSVAVGKSTTSR
                     LLQILIARTFKYAKVELVTTDGFLQPNAVLEERQLLNKKGFPESYDMEKLIDFLDKIK
                     NGYDCQIPVYSHEIYDIIPNKTQEIKSPDFLIVEGINVFQNPQNQRLYVSDYFDLSIY
                     VDADVEHIETWYLERFQKLLTLAKNDPNNYYHRFTQMTYPEILSIAQNTWKNINLANL
                     EKFIEPTRNRADIILHKAENHEIDKIYLKK"
     gene            1010..1258
                     /gene="rpsT"
                     /locus_tag="AC1PIW_000820"
     CDS             1010..1258
                     /gene="rpsT"
                     /locus_tag="AC1PIW_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIP6605776.1"
                     /translation="MEVKPLANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANP
                     SEELFRAASSAIDKAETKGLIHKNKASRDKARLASKLA"
     gene            complement(1400..1864)
                     /gene="tnpA"
                     /locus_tag="AC1PIW_000821"
     CDS             complement(1400..1864)
                     /gene="tnpA"
                     /locus_tag="AC1PIW_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISSsu4 family
                     transposase"
                     /protein_id="HIP6605777.1"
                     /translation="MAQKAHSLSHTKWLCKYHIVFTPKYRRKIIYNQYRSSLGEIFRR
                     LCTYKGVEIIEGHLMPDHVHMLVSIPPRLSVSSFIGYLKGKSALMMFDKHANLKYKFG
                     NRHFWAEGYYVSTVGLNEATIKKYIQEQEKHDIALDKLSVKEYEDPFRDNGK"
     gene            complement(2039..4048)
                     /locus_tag="AC1PIW_000822"
     CDS             complement(2039..4048)
                     /locus_tag="AC1PIW_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6605778.1"
                     /translation="MFGLTVRLALTNLKKNRRLYFPYALMTILSTAIAYIFASLAFHP
                     DLGQVKGANGVIQVLGFGMIVVMLAVAIMVFYANSFVMKQRSKEFGVYSILGLEKKHL
                     FLMTFLENLVFSVGTILAGLLLGLALDKLFYALLLKAMQMPVVLASTFQLKSLVTVFI
                     YLFGIFALVSLFNIGNLGLTDSLKLVQGKKRGDKKTGFLWLQTLLVLILLGAGYAIAQ
                     LVTSPMQAIPSFFGATLLVIFGTYLLFHAGVISLLNWLKNRQTYYYKPDNFISVSNLI
                     FRMRKNALGLATITILSSMFLVTMVGGLNIYIGGKDYIANQNPNDYSIDVGMQPTTSK
                     AQTEKWEEWAEAILEETDIPVESKVVYPYQQAYFSEVANQQVRFLTEKESWMDFSNQV
                     GVGYILDKATYEKMTSQKLELAADQVAIYSKGVQYQAGQTLTFDEKEMEVAQVLPKNV
                     TLGHLPDHVTFNFSKYLVIVVQDLTIFENQAENRYYMGFESSLSEEEQVNSQEVFLSG
                     SFESELWESNILPNGTNAISLAYRAYAMRSFFSFAGSLFFIGLLLSLIFLMAVVLVIY
                     FKQISEGYEDRDRFVIMTKVGLDEDQTRQSIRKQLLTVFFLPILLAFVHLAFAYKMLS
                     SILLSIGVVNAGLMLQVTAGICGGYLLLYLVVYAITTRSYKQIIS"
     gene            complement(4050..4808)
                     /locus_tag="AC1PIW_000823"
     CDS             complement(4050..4808)
                     /locus_tag="AC1PIW_000823"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6605779.1"
                     /translation="MSVLDVQHVQKIYSSLFKAGQVEALKDIHFAVEKGEYVAIMGES
                     GSGKSTLLNILATLDKPTQGKVLLNGLDTQSIKSKEAAAFRREKLGFVFQDFNLLDTL
                     SVKDNVLLPLVLSRYPIDEMNKRLVHTLNSLGIATLQDKMPYEISGGQQQRVAVARAI
                     ITQPEILLADEPTGALDSKSTATLLDIFEKINQEGQTILMVTHSTVAASRAKRVLFIK
                     DGILYNQIYRGERTSQEMYQLISDTLTLMANRGE"
     gene            complement(4904..5896)
                     /locus_tag="AC1PIW_000824"
     CDS             complement(4904..5896)
                     /locus_tag="AC1PIW_000824"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIP6605780.1"
                     /translation="MNKDDFGSLVPVFLTSWLGHRLIFLIAYAVFAVAILAYSSLFDI
                     KRNLVFYAILLLSICWLLALLWDFVREFSRFGKIWRGQKVTTGTASERLLQEKVERLE
                     VQNKQILEKQRQEQTELDDYYTLWAHQMKTPIAASQLLVKEVDSSPVRHQLETELFKI
                     EQYTGLVLNYLRLQSFHDDLVIESVNLDELVRNLVRKYSLFFIQVNTSLDLGQLDRTV
                     KTDKRWLGLLIEQILSNALKYCQEGTISIVLDGDDLVIRDTGIGIAESDLERVFERGF
                     SGFNGRRTQQSSGLGLYLSRKIAAELGYSLKLKSKVGQGTEVRIGIKEVELIFY"
     gene            complement(5889..6572)
                     /locus_tag="AC1PIW_000825"
     CDS             complement(5889..6572)
                     /locus_tag="AC1PIW_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIP6605781.1"
                     /translation="MQKILLIEDDKTISQLVAKNLINWGYQVQEVKDFQMVLEQMEEF
                     QPHLILLDIGLPFFNGYYWCQEIRKTSRVPIMFLSSHDQPMDIVMAINMGADDYVTKP
                     FEMTVLLAKIQGLLRRTYDFVGEQSLLWFEEISLDLKTMQVSYGQVVEELTRNEFQIL
                     RVLFEHGKEVVSREELMRELWNSDIFVDDNTLSVNIARLRKKLAELDLPDVIATKKGV
                     GYGLVWTHE"
     gene            6742..7923
                     /locus_tag="AC1PIW_000826"
     CDS             6742..7923
                     /locus_tag="AC1PIW_000826"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HIP6605782.1"
                     /translation="MPNLLTYLEETQYDNFYDKPINKLDILALTELSYLPFDNLVPYS
                     FTENGVRLDRLAAAFEETYKNNFPPFSMVTKNRLALLGLLFKSKRFKSIKAFGFVDDY
                     QLEQEKQFSAISYRIDRKTIVTCFRGTDDTIIGWKEDFHMTYMDEIPAQRAASGYLEK
                     LMMQQGGHFYLAGHSKGGNLALYAACQQATDQQDRILAIYPFDSPGLHKKHLEAPSYQ
                     AIHQRIFPVIPQNSIVGMMLETPENAQIVQSNTLGILQHISFSWEVDGSDFKIAPALT
                     SDSLQTDQALKTWTDSLTDDELRDFFDLFFGIFIKAGIERFSDITVNPLQKLQEIDRL
                     RKEIAPEEAEMVDKLIRLLFDTRYQIWRDNISTSIPSPEISLPDWRKLFQRNTTENKE
                     N"
     gene            complement(7938..9062)
                     /locus_tag="AC1PIW_000827"
     CDS             complement(7938..9062)
                     /locus_tag="AC1PIW_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HIP6605783.1"
                     /translation="MKLPRFGVEEWLNVHENSAIYDIAGVSISSLTLEELFALSGTNP
                     EDFYKKLQGTKLNYGWIEGSPAFKKSVSQLYTGVKPEQILQTNGATGANLLVLYGLIE
                     PGDHVISLYPTYQQLYDIPKSLGAEVDLWQIEEEHGWLPDLEKLRQLIRPNTKMICIN
                     NANNPTGAVMDRAYLEELAEIASEVGAYILSDEVYRSFSELDVPSIIEVYDKGIAVNS
                     LSKTYSLPGIRVGWVAANHQVTDILRDYRDYTMICAGVFDDLVAQLALAHYQEILERN
                     LRILEENLAILDQWIEEEPLVSYIRPAVVSTSFVKIAVDMPMEDFCLQLLQEYGVLLV
                     PGNRFDRDGYVRLGFACEQETLIKGLEKLSQFLRRFDKEN"
     gene            complement(9209..9970)
                     /locus_tag="AC1PIW_000828"
     CDS             complement(9209..9970)
                     /locus_tag="AC1PIW_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIP6605784.1"
                     /translation="MSNQLENAKNLYLRGIRDGEIKEVHENYMGASYTQHSTGVPDEK
                     EGFAAFFEDFFKRNPKREINIVRAIEDGNFVFVHVHQKLNDGAAEWVTADIFRSDENG
                     CIVEHWDVIDAYPKTIGQTDPIYADFELTDLDKTEDNKKIVRRFLVDVLQNGKIEKFD
                     DYVAADLIQHNQEIAQGGVAYKDYLVESQVNYDFVFKVMGQGDYVVAYSKVWIAGQDY
                     AHFDIYRLKDGKIVEHWDNKEVMPDKKDLTNLGKF"
     gene            complement(9980..11491)
                     /locus_tag="AC1PIW_000829"
     CDS             complement(9980..11491)
                     /locus_tag="AC1PIW_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HIP6605785.1"
                     /translation="MNLFETFIERKDEWGIALFEHLRISLLALIIAIAIAVPLGLILS
                     SKKRLTEWSLQITGIFQTIPSLALLGLFIPFMGIGTLPAVVALVIYAIFPILQGTLTG
                     LGEIDPSLEEAATAFGMNKWEKLKKFKLALAMPILMSGIRTASIMIIGTATLAALVGA
                     GGLGSFILLGIDRNDSALILIGAVSSAVLAVLFGYGIRLLQDKKPKTILLALLVTLFT
                     VGASYVPMLNFSTKQLVIAGKLGAEPEILINMYKLLIEDQTDIKVEIKPNFGKTSFLY
                     EALKSGSIDIYPEYTGTITSTLLKNSPMDLSTNPEEVYTYAKEAILEQDGLMYLAPMA
                     FQNTYALAVTEDYAQKNGLEKISDLAKVQQTAVAGFSLEFNDREDGNIGLKNLYNLQL
                     NVKTMEPALRYEAIKSGNVQIIEAYSTDSKVVTYKLKILEDDKQLFPPYQAAPLLSKE
                     TLEQYPELEQVLGVLAGNISTEEMTQMNYAVDVEGKSAEQVAREYLEQEGLLQ"
     gene            complement(11491..12222)
                     /locus_tag="AC1PIW_000830"
     CDS             complement(11491..12222)
                     /locus_tag="AC1PIW_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIP6605786.1"
                     /translation="MIDYQNVSLTCKVSGPILKNLTFDIQEGEFFVLIGPSGSGKTTT
                     LKLINRLIEQTEGEVFFQGKRLKDFDLRELRLETGYVLQQIALFPNMTVAENIALIPE
                     MKGLGKEETLTRMRELLTKVGLEPDSYLDRLPKDLSGGEKQRIGILRAIIANPKVLLM
                     DEPFSALDPISKAQLQDLIKELHEEFKMTTVFVTHDMDEAVKLADRICLMQDGQVVQL
                     GSPDELRNRPANDFVKEFIRARGGM"
     gene            complement(12215..12664)
                     /locus_tag="AC1PIW_000831"
     CDS             complement(12215..12664)
                     /locus_tag="AC1PIW_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIP6605787.1"
                     /translation="MMEVEKRSKDYARMFDQQVGLYEDYARGHGLNAKCLSILMWIYY
                     NPGGVTQNWVSKKTYSSKQVVNATVKKFLDGGLVVLEENPADKRHKKIKLTEEGQQFA
                     SRILDPLEEAENKALSQLSQEEQELMLGLIQRYSQSLMAILKGEDDD"
     gene            complement(12975..13511)
                     /locus_tag="AC1PIW_000832"
     CDS             complement(12975..13511)
                     /locus_tag="AC1PIW_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HIP6605788.1"
                     /translation="MVKKSSSVWTSLLLGAAGGAAAAAFLASKTGKTVKEKVVNFAND
                     YKENHEEINADFVTKAQDLGKQATERFTEVKTQLETGELTVEDLVKSGKEKSLETFEQ
                     IKEKIAEQNLSTADILEAIKAKTAKAPTVDLTEEDIEDAVVVSEDIEIDIEDVQTEVV
                     SETVSEVASETTSEAVEG"
     gene            complement(13495..13905)
                     /locus_tag="AC1PIW_000833"
     CDS             complement(13495..13905)
                     /locus_tag="AC1PIW_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HIP6605789.1"
                     /translation="MIEISVLIIALSIAAVAVYIVLLLKKLGTVTDEAQQTLKVLTSD
                     VNVTLYQTNELLAKTNVLVEDVNGKVSTLDPLFVAVADLSTSVSDLNASARDLTVKAK
                     SAGASTVKAGGALSALSTLSSLLGKKGEKNGKKI"
     gene            complement(13919..14695)
                     /gene="lgt"
                     /locus_tag="AC1PIW_000834"
     CDS             complement(13919..14695)
                     /gene="lgt"
                     /locus_tag="AC1PIW_000834"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIP6605790.1"
                     /translation="MDPIAIKLGPLEIRWYAICILLGLILGVYLATKEGPRKKIRQDD
                     ILDFILIAFPLSILGARIYYVAFSWSEYKDNILSIFAIWNGGIAIYGGLITGAIVLYF
                     FTQYRFINTLDFLDIVAPSVMIAQAIGRWGNFFNQEAYGKAVESLNYLPAFIRDQMYI
                     DGAYRQPTFLFESLWNLLGFGLVCVLRRRPKFLKQGEITAFYLVWYGCGRLLIEGLRT
                     DSLMFLGIRVSQWLSGVLILVGIIMVVLRRRKSSIPFYQP"
     gene            complement(14697..15629)
                     /gene="hprK"
                     /locus_tag="AC1PIW_000835"
     CDS             complement(14697..15629)
                     /gene="hprK"
                     /locus_tag="AC1PIW_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HIP6605791.1"
                     /translation="MTVYVKDLVDNLRIECVYSTEELLQKQITTADITRPGLEMTGYF
                     DYYAPERIQLIGMKEWSYLMAMTAHNRYQVLSQMFQPETPVVIVARGLEIPEEMYKAA
                     KEKQIAICRSKTATSRLSGELSSYLDSRLAQRTSVHGILMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDVYAKDDVTLWGEPAEILRHLLEIRGVGIIDIMSLYGAS
                     AVKDSSEVQLAVYLENFETGKVFDRLGNSGDTIEIAGIAIPQIRIPVKTGRNISVVIE
                     AAAMNYRAKQMGFDATKIFEERLSNLIEHNREEA"
     gene            15843..16787
                     /gene="manA"
                     /locus_tag="AC1PIW_000836"
     CDS             15843..16787
                     /gene="manA"
                     /locus_tag="AC1PIW_000836"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HIP6605792.1"
                     /translation="MMEPLFLTPVMHEKIWGGNRLRTNYHYDIPSDKTGECWAISAHP
                     NGVTTVSNGQYKGRGLDDLYKNEKHLFGNPTDDVFPLLTKILDADDWLSVQVHPDDSY
                     GLAHEGELGKTECWYILEAEEGAEIIYGHNAQSKEELRQQIEAGDWDKLLTHVPVKKG
                     DFFFVPSGTMHAIGKGILILETQQSSDTTYRVYDFDRRDDAGNLRELHIEKSIDVLTI
                     GPVANSTPAHLKAGNLDSTLLVANPFFTVYKWNIQQEIKMKQTVPYLLVSVIEGEGAI
                     QVGETSYPLQKGSHFILPANVTDWTFTGQMDIIASHAN"
     gene            complement(16830..>16990)
                     /locus_tag="AC1PIW_000837"
     CDS             complement(16830..>16990)
                     /locus_tag="AC1PIW_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HIP6605793.1"
                     /translation="GLLLGKSFLLLIALYIAAAYFLPYKEDRDAERYGTGPRKIKEAE
                     KINKSWFE"
BASE COUNT         5064 a         3976 c         3174 g         4776 t
ORIGIN      
        1 tgaaacgctt acctatagga agaattatgt atgaaaaatg aatttttaaa ttttgaacaa
       61 attgaccgag ccacttggca acaactacat cgtaagacta ccattccact tagtcaaagc
      121 gaactaaatt ctattaaaag ttttaacgac cgcatccaat tacatgaggt ttctgacatc
      181 tatcttcctt tggttaatct tatccatatc tatcggaaag cacgcaaaga cctaaatttt
      241 acgaagagtt tatttctaca gaagactatc aagccacaac catttatcat tggcgtttct
      301 ggaagtgttg ctgtcggcaa atcaacaact agtagattgc tgcaaattct tatcgctcgg
      361 acatttaaat acgccaaggt tgaattagta acaacagatg gttttctcca gccaaatgcg
      421 gttttagaag aacggcaact gttaaataaa aaggggtttc ctgagtccta tgacatggag
      481 aaattgattg atttccttga taagataaaa aacggctacg actgccaaat ccctgtctat
      541 tcacatgaaa tctatgatat tatccccaac aaaactcagg aaattaagtc tccagatttc
      601 ttgattgttg aaggaatcaa tgtatttcaa aatccacaaa accagcgcct ctatgtcagt
      661 gactattttg acctgtctat atacgttgac gctgatgtgg agcatattga aacctggtac
      721 ttggagcgat tccaaaaatt attgacattg gcaaaaaatg accctaacaa ctattaccat
      781 cgttttaccc agatgacgta ccctgaaatt ctatctattg cccagaatac ttggaaaaac
      841 attaatctag ctaacttaga aaaattcatt gaaccaacca gaaaccgtgc tgatattatt
      901 cttcataagg ccgaaaacca tgagattgat aaaatttatc taaaaaaata aatttcactt
      961 gtcaaaaata ctcttttcag atataatggt gtagttagta caagaaaggg tggaggtgaa
     1021 accattggca aacattaagt cagctatcaa acgcgctgaa ttgaacgtta aacaaaacga
     1081 gaaaaactca gcacaaaaat cagctatgcg tactgcaatc aaagcatttg aagctaaccc
     1141 atctgaagag cttttccgtg ctgctagttc agcaatcgat aaagcagaaa ctaaaggttt
     1201 gattcacaaa aacaaagcaa gccgcgataa agcacgtctt gcatcaaaac ttgcttaatc
     1261 atgaccaccc gtcaaacggg tggtttgaac aagggctata agcccacatc accagccagc
     1321 gcctaaagac gctggctttc actttgttca agcctcactg cttttgactc gtcactagcc
     1381 tcttaaagag gcattcgtac tacttgccat tatccctaaa gggatcttca tactctttta
     1441 cactcaactt atcaagtgct atatcatgtt tttcctgttc ttgtatatat ttcttaattg
     1501 tggcttcatt tagtccaacc gtacttacat aatatccctc tgcccagaaa tgacgattac
     1561 caaatttgta tttgagattg gcgtgtttat caaacatcat tagagcactc ttacctttta
     1621 aatatcctat aaagcttgat acacttaatc gcggaggaat actgactaac atatgaacat
     1681 gatccggcat cagatgtcct tcgataattt ctacgccttt ataagtacac aaacgtcgga
     1741 atatttctcc caaactactt cgatattgat tatagatgat ttttcgtcta tacttaggtg
     1801 tgaagacaat gtggtattta cacagccact ttgtatgtga taaactatgt gccttttgcg
     1861 ccatattttt ctcctttcgc tttacaatag gcttgaacac ctttattgta tcgcgtttgg
     1921 agtttttttg gtgtaacctt cgtcgcgcac ccgcatagcg ggtggtttat ttgtcacgca
     1981 ccttacggag cgtgacggac taaaagtcac ataattaaaa acttgggttc ccccaagttt
     2041 aggaaataat ctgtttgtaa gatcgtgttg tgatggcata gaccactagg tagaggagga
     2101 gatagccacc acagatgcct gcagtcacct gcaacataag ccctgcattg accacaccga
     2161 tagacaaaag aatggacgag agcatcttgt aggcaaatgc aagatggaca aaggcaagta
     2221 ggataggtag gaaaaagacg gtcagcaatt gcttgcgaat ggactgtctg gtctgatcct
     2281 catccagacc aaccttggtc ataatgacaa agcgatctct gtcttcatag ccttccgaaa
     2341 tctgtttgaa gtaaatcacc agcaccaccg ccatcaaaaa aatgagtgaa agtaagaggc
     2401 cgatgaagaa aagtgaacct gcaaaactga agaaactacg catcgcatag gcacggtagg
     2461 cgagactgat ggcgttcgtt ccattcggta atatattgga ctcccaaagc tcactttcaa
     2521 aactaccgga taagaagacc tcctggctat ttacctgttc ttcttctgat agactgcttt
     2581 caaagcccat ataatagcga ttttcggctt gattttcaaa tattgtcaag tcttgaacaa
     2641 caataactaa atacttacta aaattaaaag tcacatggtc tggcagatgt cccaaggtca
     2701 cattcttggg caagacttgg gcaacttcca tctccttttc atcaaaggtg agggtttgac
     2761 ctgcttgata ctggactccc ttactataaa tggccacttg gtcagctgcc aactccagtt
     2821 tttgactagt cattttttca tatgtagctt tatccaaaat atagcctaca cctacttgat
     2881 tagaaaaatc catccatgac tctttttccg tcaaaaatct tacttgctga tttgccacct
     2941 cagaaaaata ggcttgctga taaggataga ctaccttact ttcaacagga atatctgtct
     3001 cttctaagat agcttccgcc cactcctccc acttctccgt ttgagctttt gaagtggttg
     3061 gctgcatacc aacatcaatc gaatagtcat ttggattttg attggcaata taatctttgc
     3121 caccgatgta aatattgagt cctccaacca tggtcaccaa gaacatgctg gagaggatag
     3181 taatggtcgc aagacccagt gcatttttcc gcatacggaa aatcaagttg gatacagaaa
     3241 tgaaattatc tggcttataa taataggtct gacggttctt tagccagttt aagagactga
     3301 tgacacctgc gtggaagagc aggtaggtcc caaagataac cagaagagtc gctccaaaga
     3361 aacttggaat agcctgcatc ggactggtca ccagctgggc aatggcgtag cctgccccca
     3421 ataagataag aactaagagg gtttgtagcc agaggaagcc cgttttcttg tcccctcgct
     3481 tcttaccttg aaccagtttg agtgagtcgg tcaagcccag atttccaata ttaaaaaggc
     3541 tgaccaaggc aaaaatccca aagagataga taaatacagt gactagactt ttcaactgga
     3601 aggttgaagc caggaccaca ggcatctgca tggctttcag caagagcgca tagaagagtt
     3661 tatctagagc cagtcccaag agcaagccag ctagaatagt accgactgaa aagaccagat
     3721 tttctagaaa agtcatcaag aagagatgtt ttttctccag accaagaatg ctatagacac
     3781 caaactcttt tgaccgctgc ttcataacaa aactattggc atagaagacc atgatggcaa
     3841 cggccagcat aactacaatc ataccgaagc ccagcacttg aatcactcca ttggccccct
     3901 tgacctgacc taaatcagga tgaaaggcaa gtgaggcaaa aatataggca atggctgtgg
     3961 acaaaatggt catcagggca tagggaaaat agagtctgcg gttctttttg agattggtca
     4021 gagccaatcg aacggttagt ccaaacatac tactcacctc gattcgccat aagtgtcaag
     4081 gtatctgaaa tcaactgata catttcctga ctagttctct cacctcggta gatttgattg
     4141 tagaggatgc catccttgat gaagagaacc cgtttggcac gactagctgc tactgtcgag
     4201 tgggttacca tcagaatcgt ttgcccctct tggttgattt tttcaaaaat atctagaaga
     4261 gttgccgttg atttagagtc gagggcccca gtcggctcat ccgctaagag aatttctggc
     4321 tgggtgatga tggcacgcgc caccgcaact cgctgctgct gaccacccga aatctcgtag
     4381 ggcatcttgt cttgcaaggt cgcaattccc aggctattga gcgtatgaac gagtcgcttg
     4441 ttcatctcat caatcggata gcgagacaaa accagaggca agagcacatt atccttgaca
     4501 gaaagagtat ccaagagatt gaaatcttgg aagacaaagc ccaatttctc ccgacgaaag
     4561 gctgccgctt ccttgctctt gatagattgg gtatccaatc cgttaagcaa gaccttgccc
     4621 tgcgttggct tatcaagggt tgccaaaata ttgagcaagg tggacttacc agaacctgac
     4681 tcacccataa tagcgacata ttcacccttt tccactgcaa aatgaatatc cttcaaagct
     4741 tcaacctgtc ccgccttaaa gagagaactg tatatctttt gaacgtgttg aacatctaaa
     4801 actgacataa cttgactcct tttctttttc taccctcatt ctacacggtc ccaagacaaa
     4861 ctaccatagt cctagctttc atttgcgtga cagttttgta agattagtaa aaaatcaact
     4921 ccacttcttt gataccgatt cgcacctctg ttccctgacc tacttttgat tttagcttta
     4981 gggaatagcc cagctccgca gcaattttcc gtgagagata gagccccaga cccgaagatt
     5041 gctgggttct gcggccattg aaacctgaga agccacgctc aaagacccgt tccaagtcac
     5101 tctctgcaat cccaattccc gtatcgcgaa taaccaaatc atctccgtcc aaaacaatcg
     5161 aaatggtccc ttcctgacaa tacttgaggg cattggataa aatttgctct atcaacaggc
     5221 ccagccagcg tttgtcggtc tttactgtcc tatccaactg ccccaagtcc aagctagtat
     5281 tgacctgaat gaaaaagagg gaatactttc ttactagatt tctgaccagt tcatccaaat
     5341 taactgattc gataaccaaa tcatcatgaa aggactgtaa gcggagatag ttcaagacca
     5401 gacctgtgta ttgctcaatc ttgaagagtt ctgtttctag ctgatgacgg actggactac
     5461 tatctacttc cttaaccaag agctgactgg ctgcaatcgg cgttttcatc tggtgggccc
     5521 agagggtgta gtagtcatct aattctgtct gttcctgtcg ctgtttttct aggatttgct
     5581 tgttctggac ttctagtcgc tccacttttt cctgcaacaa acgctcactg gctgtcccag
     5641 tagtcacttt ctgaccacgc cagatttttc caaagcggga aaattccctg acaaaatccc
     5701 agaggagggc gaggagccaa caaatactga ggaggaggat ggcatagaaa accaagtttc
     5761 ttttgatgtc aaagaggcta ctataagcca gaatagccac agcaaaaacg gcgtaggcta
     5821 tcagaaaaat caagcgatga ccaagccatg atgtcaaaaa tactggtact aatgagccaa
     5881 aatcatcctt attcatgtgt ccacaccagg ccgtagccta ctcctttctt ggtcgcaatc
     5941 acatctggca gatccagttc tgccaatttc ttacgcaggc gagcgatgtt aaccgacagg
     6001 gtattgtcat ccacaaaaat atcactgttc cagagttctc tcatcagctc ctcacggctg
     6061 accacttcct tgccatgttc aaataaaacg cgtaaaatct gaaattcatt tcgcgtcagc
     6121 tcttccacaa cctgtccata ggacacctgc atggtcttta ggtctaggga aatctcctca
     6181 aaccagagta aactttgttc tccgacaaag tcataggttc ttctaagaag cccctgtatt
     6241 ttggccagga gaaccgtcat ttcaaagggc ttggtcacat agtcatccgc ccccatattg
     6301 atcgccataa caatatccat tggctggtca tgggaagaca gaaacataat gggcacacgg
     6361 gatgtcttgc gaatttcctg acaccagtaa tagccattga aaaacggcag accaatatcc
     6421 agtaaaatca aatggggctg aaactcttcc atctgttcca aaaccatctg aaaatccttg
     6481 acctcttgca cctggtaacc ccagtttatc aagtttttag caactagttg gctaatcgtt
     6541 ttatcatcct cgataagaag aattttctgc ataactatct cctttcatat ccctactatt
     6601 tattatagct tacattggaa caaatttgag tatttttata catttacaca agaagtagac
     6661 tataaaacca ggtcatcagt ggtgcagatt tgtcagacca atcatatttc gttatactag
     6721 ataaaaaagg aaaggactgc tatgccgaat ttattaactt atcttgaaga aactcaatac
     6781 gataattttt atgataaacc tatcaataag ctagatattc tggctctgac cgaactttcc
     6841 tacctccctt ttgataatct ggtcccctac tcttttacag aaaatggtgt acgacttgat
     6901 cggttggcag cggcctttga agagacctac aaaaataatt tccctccctt ttctatggtg
     6961 accaaaaacc gcctagcctt actaggactt ctattcaaat ccaaacgctt caaatctatc
     7021 aaggcatttg gcttcgttga cgattatcag ttggaacagg aaaagcagtt ttcagcaatc
     7081 agctatcgca ttgaccgaaa aacgattgtg acttgcttca gagggacaga tgatactatt
     7141 attggttgga aggaagattt ccacatgacc tatatggatg aaattcctgc ccaacgtgca
     7201 gcgagtggct acttagaaaa acttatgatg cagcaggggg gccattttta tctggccggt
     7261 cattcgaaag gtggaaattt agccctctat gcagcatgcc agcaagctac agaccaacag
     7321 gatagaattc tagctatcta tccctttgat tcacctggct tacacaaaaa acacttggaa
     7381 gcacctagtt accaagctat tcaccaacgg attttccctg tcattcccca gaattcaatc
     7441 gtcggtatga tgttagaaac accagaaaat gctcagattg tccagagtaa taccctcggc
     7501 attctacaac acatcagttt ttcttgggag gttgacggaa gcgacttcaa aatagcccct
     7561 gccctgacca gcgatagcct acaaacagac caagccttaa aaacttggac ggatagttta
     7621 acagacgatg aattacgaga cttcttcgat ttgtttttcg gcatcttcat caaagcagga
     7681 attgaacgat ttagcgacat cacggtaaat cccctacaga aattacagga aattgatcga
     7741 ctgagaaaag aaatagctcc agaagaagcc gaaatggtag acaaactcat tcggctcctc
     7801 tttgacaccc gctaccaaat ttggagggac aatattagca cttccattcc aagccctgaa
     7861 atctctctac cagattggcg gaaacttttc caaagaaata caactgaaaa taaagaaaac
     7921 tagctcaacc acttgagcta gttttcttta tcaaatcttc ttaaaaattg agataatttt
     7981 tctaatcctt tgattagagt ttcttgttca caggcaaaac ctagacggac atagccgtca
     8041 cgatcaaaac gattgccagg aactagaaga acaccgtatt cctgcagaag ttgcaagcaa
     8101 aaatcttcca taggcatatc cactgcaatc ttgacaaagc tagtggaaac aaccgctgga
     8161 cggatatagg aaacgagggg ttcttcctca atccattggt ccaaaattgc taaattttct
     8221 tctaaaatgc gcagatttcg ttctagaatt tcttgataat gggccaaagc taattgagcc
     8281 accagatcgt caaagacacc tgcacaaatc atagtgtaat cccgataatc tctcagaatg
     8341 tccgtcactt gatgattggc ggccacccaa cccacgcgaa tacctggcag agaataggtc
     8401 ttggataagc tatttacagc aattccttta tcatagactt ctataataga tggcacatcc
     8461 agctcagaaa aggaacgata gacttcatct gataggatgt aagccccaac ttcactagca
     8521 atttccgcca attcttccaa ataagcccta tccatgacag cgccggttgg attattggca
     8581 ttgttgatgc aaatcatctt agtgttagga cgaatgagct gacgcagttt ttccaagtct
     8641 ggcaaccatc catgctcttc ctcaatctgc cacaaatcca cttctgctcc caaagacttt
     8701 ggaatatcgt agagttgttg gtaggtcgga taaagggaaa tgacatggtc tcctggctca
     8761 atcaaaccat aaagaactaa caagtttgcc cctgttgccc cgttggtctg gagaatttgt
     8821 tctggcttga cgcctgtata gagttgacta accgattttt taaatgctgg cgaaccttct
     8881 atccagccat agttgagctt ggtgccctgt aatttcttat aaaaatcctc aggattggtg
     8941 cccgacaagg caaatagttc ctccaaagtc aatgaagaaa tagacactcc tgcaatatca
     9001 taaatcgcgc tgttttcatg gacattcagc cattcctcaa cgccaaaacg tggtaatttc
     9061 ataagacttc ctacttttat ttattctacc cttattgtac catattcttt caataattgt
     9121 ataaatatgc aacataacaa gtaaagaata ttactattat taaaaaacag actttctcaa
     9181 acgagactgc ctgctctgct tttttccctt aaaacttccc taaattcgtc aagtcttttt
     9241 tgtctggcat gacttccttg ttgtcccagt gctcgacaat ctttccgtct ttgaggcggt
     9301 agatgtcaaa gtgagcgtag tcctgacctg caatccagac tttcgaataa gccaccacat
     9361 agtcaccctg tcccatcacc ttaaagacaa agtcgtagtt gacttgactc tctaccagat
     9421 agtccttata ggctacaccg ccttgggcga tttcttggtt gtgctgaata agatcagctg
     9481 ccacatagtc gtcaaatttc tcgattttcc cattttgaag gacatcgacc aagaaacgac
     9541 ggacgatttt cttgttgtcc tcagtcttat ctaggtcagt cagctcaaag tctgcgtaaa
     9601 tcgggtctgt ttggccaatg gtttttggat aggcatcgat aacatcccaa tgctcaacga
     9661 tacaaccatt ttcatccgaa cggaaaatgt ctgccgtcac ccactcagcc gcaccgtcat
     9721 tgagtttctg gtggacatgg acaaagacaa aattcccgtc ctcaatggca cggacgatat
     9781 tgatttcccg cttgggattg cgtttgaaaa agtcctcaaa gaaagccgca aagccctctt
     9841 tctcatcagg cacacccgta ctgtgctggg tatagctggc tcccatatag ttttcatgaa
     9901 cttccttgat ttcgccatcg cgaatgccac gaaggtaaag atttttagca ttttctagtt
     9961 gatttgacat gattttctct tattgtaaca atccttcttg ttctaaatat tccctcgcca
    10021 cttgctctgc cgacttgcct tccacatcga ccgcatagtt catctgggtc atttcctcgg
    10081 tcgagatgtt gcctgccaag acccctagga cttgctccaa ctcaggatat tgttctaagg
    10141 tttctttgga caatagcgga gcggcctggt aaggtgggaa gagctgtttg tcgtcttcca
    10201 ggattttgag cttgtaggtg accaccttgc tgtctgtaga atatgcttcg ataatctgca
    10261 cattaccact cttaatagct tcataacgaa gggctggctc catggtcttg acattgagtt
    10321 ggagattgta gagatttttt agaccgatat taccatcttc gcggtcgtta aactctaaag
    10381 agaagccagc gacagctgtc tgctgcactt ttgccaaatc cgaaatcttc tcaagaccgt
    10441 ttttctgagc ataatcttcc gttacagcca aagcataggt attctggaaa gccattggtg
    10501 ctaaatacat cagaccatcc tgttccaaaa tcgcttcttt agcgtaggta taaacctcct
    10561 caggattcgt tgataaatcc attggtgaat ttttcaaaag agtacttgta atggtccctg
    10621 tgtactcagg ataaatatca atagaacctg atttgagtgc ctcataaagg aagctagtct
    10681 ttccgaaatt aggttttatc tccaccttga tgtctgtctg gtcctcaatc agcaacttat
    10741 acatattgat gagaatttca ggttctgcac cgagcttccc tgcaatgacc aactgttttg
    10801 tggaaaaatt aagcatggga acataactag ctccaacagt aaataaagtc accaatagag
    10861 ccaataaaat tgtcttgggt ttcttgtcct gcaacagacg aatgccataa ccaaaaagca
    10921 ctgcaaggac agccgacgat accgcaccaa tcaaaatcag ggctgaatca ttgcggtcaa
    10981 ttccaagtag aataaaggag cccaggcccc cagcccccac caaggccgcc agagtcgccg
    11041 ttccgataat cataatggaa gccgtccgaa tccctgacat caaaataggc atggcaagag
    11101 ctaacttaaa ttttttcaac ttctcccatt tattcatacc aaaagcggtt gctgcttctt
    11161 ccagagaagg gtcgatttct ccaagtcctg tcaatgtccc ctgcaagata ggaaagatag
    11221 cataaataac aagtgctact actgctggca aggttccgat tcccataaaa ggaatgaaaa
    11281 gaccaagcaa agcaagggat ggaatagttt ggaagatacc tgttatttgc agactccact
    11341 cggtcaagcg ttttttacta gatagaatca gtcccaaagg tactgcaatt gcaatcgcaa
    11401 taatcaaagc tagaagggag atgcgaaggt gttcaaaaag agcaattccc cactcgtcct
    11461 tgcgttctat aaaggtttca aataggttca ttacatacct cctcgagctc tgataaattc
    11521 cttgacaaaa tcattggcag gacgattcct caattcatca ggacttccta attgcacaac
    11581 ttgtccatct tgcatgagac agatgcgatc cgccaattta accgcctcat ccatatcatg
    11641 ggtaacgaaa actgttgtca tcttgaattc ctcatgtaat tccttaatca agtcctgcaa
    11701 ctgagcctta ctgattggat ccagagctga gaaaggttcg tccatgagta acactttcgg
    11761 atttgcaatg atggcccgca aaatcccgat ccgctgtttt tctcctccag acaagtcctt
    11821 gggcaaacga tccagatagc tgtctggttc caagccaacc ttggtcaata gctctctcat
    11881 cctcgtcagg gtttcttcct tgcccaaacc tttcatttca ggaataagag caatgttttc
    11941 agctaccgtc atatttggaa aaagggctat ctgctgaaga acataacctg tctccaaacg
    12001 taattcacgc aggtcgaaat ccttaagtcg cttaccttga aaaaaaacct ctccctcggt
    12061 ctgctcaatt agacggttaa tcaatttcaa cgtagttgtc tttccgctac cactcggtcc
    12121 aatcaagaca aagaactccc cttcttgaat atcaaaggtc aaatttttca agattggtcc
    12181 actaacctta caggtcagag atacattttg ataatcaatc atcatcttct ccttttaaaa
    12241 tagccatgag actctgactg tagcgctgaa ttaatcctag cataagctcc tgttcctcct
    12301 gactgagttg agacagcgcc ttattttccg cctcctctaa gggatccaaa atgcgactag
    12361 caaattgttg cccctcctct gtcaatttaa ttttcttatg tcgcttatct gctggattct
    12421 cctctaaaac taccaggcct ccatccaaaa atttctttac agtagcattg acaacttgtt
    12481 tgcttgaata ggtcttctta ctgacccagt tttgcgtcac acctccggga ttataataaa
    12541 tccacatgag aatggataaa cattttgcat tgagtccatg tccacgagca tagtcttcat
    12601 aaagaccgac ttgctggtca aacatacgag cataatcctt actccttttc tcaacttcca
    12661 tcatccttcc ccctcctcag tgagcacaac taccttacta aaagcctctg gtctactagt
    12721 gttcaaaaca acaacaattt tcacaacgac ttcctcctgt aatcatgtta gtcaatattt
    12781 tttactattt taccaaaaaa agaaaaatag tcaatcattt tgactatttt gagacatcaa
    12841 ctaccttatt atagattccc gtcttcatcc attcaaacat attaaatccc tgaaattatc
    12901 tgcttttaat ttacacaaaa caaatgataa tcagataaca aaactagcct agaaaattct
    12961 aagctagttt cattttatcc ttcaactgct tctgaggtag tttcgcttgc tacttctgaa
    13021 acagtttctg ataccacttc agtttgaaca tcttcgatat caatttcaat atcttcagac
    13081 acaactacag catcctcaat atcttcttct gtcaaatcca ctgtcggagc tttagcagtt
    13141 tttgccttga tagcttccaa aatgtcagcc gttgataagt tttgttccgc aattttttcc
    13201 ttgatttgct caaaggtttc caaggatttt tccttaccag acttgaccaa atcttctact
    13261 gtcaattcac cagtttcaag ttgagttttt acttcagtaa atcgttcagt cgcttgtttg
    13321 cctaaatctt gtgccttagt gacaaaatca gcattgattt cttcatggtt ttctttatag
    13381 tcattagcaa agtttacaac tttttctttg actgttttac cagttttact tgctagaaaa
    13441 gcagctgcag cagctccgcc ggctgcccct aacaaaaggc tagtccacac gctactagat
    13501 ttttttacca tttttttctc ctttcttacc taagagagat gagagagttg acagagctga
    13561 aagagctccg ccggctttta cagtacttgc tccagcagac ttggctttga ctgtcaaatc
    13621 acgcgctgaa gcattcaaat ctgatacaga agttgacaag tcagctaccg ctacaaaaag
    13681 aggatcaagg gtcgaaacct tgccatttac atcttcgact aaaacattgg ttttagccaa
    13741 aagctcgttg gtctgataga gagtcacatt gacatcgctg gtcaagacct taagcgtttg
    13801 ctgggcttca tccgttaccg tacccaattt tttcaacaac aaaacaatat atactgccac
    13861 cgccgcgatg gacaaggcaa taatcaagac agaaatttca ataatcatat cttctccttt
    13921 agggttgata gaatggaata gaagactttc ttctccgcaa cacaaccatg ataataccaa
    13981 cgaggattaa caccccagat agccattgtg aaacacgtat tcccaagaac atgaggctgt
    14041 ccgtccgcaa gccttctatc aaaaggcgac cacaaccata ccagactagg tagaaagctg
    14101 taatttctcc ctgtttaagg aattttggcc gtctacgtaa cacacaaacc aagccaaagc
    14161 ctagcaaatt ccagagagac tcaaacaaga aggttggttg acggtaggca ccatcaatat
    14221 acatctggtc tcggataaag gctggcaggt agttcaaact ttctactgcc ttaccatagg
    14281 cctcttggtt aaagaaattt ccccaacgac ctattgcctg agcaatcatg accgatggcg
    14341 ctacaatatc caaaaaatcc agggtattaa taaagcgata ctgggtaaag aaataaagga
    14401 cgattgcacc tgtaatcaaa ccaccataga tggcaatacc accgttccag atagcgaaga
    14461 tagataaaat gttatcctta tattcactcc atgaaaaggc gacgtagtaa atccgtgccc
    14521 ctaggataga aagcggaaat gcgattagaa taaaatctaa aatatcatcc tgacgaattt
    14581 tctttcgcgg accctctttt gtcgccagat aaacacctag aatcagacca agcaaaatgc
    14641 agattgcata ccaacgaatt tctaagggac ctaatttaat cgcaatagga tccatattaa
    14701 gcctcttctc tattgtgttc aattagatta gacaagcgtt cttcgaagat cttcgttgca
    14761 tcaaacccca tctgtttggc acggtagttc atggctgctg cttcaattac gacagaaata
    14821 tttcgaccgg tttttacagg aatacgtatt tgtggaatag caattcctgc tatttcgata
    14881 gtatcgccag agtttcccaa acggtcaaat accttacctg tttcaaaatt ttcaagatag
    14941 acagctaatt gcacttctga tgaatctttc acggcactag caccgtatag actcatgatg
    15001 tcgataatgc cgacccctcg aatttctaat aagtggcgca gaatttccgc aggttcaccc
    15061 cagagagtaa catcatcttt tgcatagaca tcaacacggt catcagccac caatcggtgt
    15121 cctcgtttaa ccaactcaag accagtctca ctcttaccga taccagaatc accctggata
    15181 agcacaccca taccgtaaat gtccatcaag ataccgtgta cactcgtccg ctgagccagg
    15241 cgactatcca agtaagaaga caattctccc gatagacgac tggttgctgt cttgcttcgg
    15301 cagatagcaa tttgtttttc ttttgcagcc ttgtacatct cctcagggat ttccaagcca
    15361 cgtgccacta tgacaacagg ggtttctggc tggaacatct gagaaagaac ctgatagcgg
    15421 ttatgggcag tcatggccat caaataagac cactccttca tcccaatcaa ctggatgcgc
    15481 tctggagcat aataatcaaa atagccagtc atttccaatc ctggtcgggt aatatctgcc
    15541 gtcgtaattt gcttttgcag gagctcttcc gtactgtaaa cacattcaat ccgcagatta
    15601 tcgaccaaat ctttcacata aacggtcatt tcttccctca ctttcagttt tattatatta
    15661 taccataagc taggggttgg gggaaaaaca agtggctgat ttggcaaaaa gaatgagttc
    15721 tttctttttc tcacaaaatt agcaaaactt ccacagatta aatgtacttc tcttccttcg
    15781 ctacatattt ttaacaaatc tgttatactt tatatagatc aatacttact actaaggaga
    15841 aaatcatgga acccttgttt ctcacacctg ttatgcacga aaaaatttgg gggggcaatc
    15901 gcttaaggac caactaccac tacgacattc ctagtgataa gactggggaa tgttgggcaa
    15961 tctctgccca tccaaatggc gtaactactg tctcaaacgg tcaatataaa ggaagaggac
    16021 tggatgacct ctataagaat gaaaaacatt tatttggcaa tccaacagat gatgtctttc
    16081 cattattaac aaaaatcctt gatgcagacg attggcttag cgtccaagtt catcccgatg
    16141 atagctatgg cttggctcac gaaggggaac tgggcaagac agaatgttgg tacattttgg
    16201 aagccgagga aggcgctgaa atcatttatg gtcacaatgc ccaatccaaa gaagaactcc
    16261 gtcagcaaat cgaagccgga gattgggaca agctgttgac ccatgtaccc gtcaaaaagg
    16321 gtgatttctt ctttgtacca agcggcacca tgcacgccat cggtaaggga atcctgattt
    16381 tagaaaccca acaatctagt gatacaacct accgtgtcta tgactttgac cgccgtgacg
    16441 atgcaggcaa cctccgtgaa ctacacattg agaaatcgat tgacgtgctg accatcggac
    16501 ctgttgctaa ctcaacacct gcccacctca aagcaggcaa tcttgactcg acacttctgg
    16561 ttgccaatcc cttctttacc gtatataaat ggaatatcca acaagaaatc aaaatgaaac
    16621 agacagttcc ttacctgctt gtcagtgtca ttgaagggga aggtgccatc caagtcggtg
    16681 aaaccagcta cccacttcaa aaaggaagcc actttatctt accagccaat gtgacagact
    16741 ggacatttac aggccaaatg gacatcatcg ccagtcacgc gaactaagca tacaaaaaac
    16801 ttgagccttt aaactcaagt ttttttgcat tattcaaacc aagacttatt tatcttttcc
    16861 gcttctttta tcttacgtgg acctgttcca tagcgttccg cgtccctatc ttccttataa
    16921 ggtaaaaaat aagctgctgc aatatagaga gcaattagaa ggaggaagga tttgcctaag
    16981 aggagacctg
//
LOCUS       DBNFCK010000032        16604 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.032, whole genome shotgun sequence.
ACCESSION   DBNFCK010000032 DBNFCK010000000
VERSION     DBNFCK010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 16604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16604
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.032"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..513
                     /locus_tag="AC1PIW_000838"
     CDS             <1..513
                     /locus_tag="AC1PIW_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HIP6605794.1"
                     /translation="KQKYYQSPAEIASFSPRPSLADSKAVLNQAVADLSVAHSILHQV
                     HWYMRGRGFMIWHPKMDEYMEEIDGYLDEMSERLITLGGAPFSTLKEFSENSQLKEVL
                     GDYNVTIEEQLARVVEVFRYLAALFQKGFDVSDEEGDSVTNDIFNVAKASIEKHIWML
                     QAELGQAPKL"
     gene            874..1752
                     /locus_tag="AC1PIW_000839"
     CDS             874..1752
                     /locus_tag="AC1PIW_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6605795.1"
                     /translation="MIEVQALEKIIKGRTILSTISFEIKSGECVALIGPNGAGKTTLM
                     SCLLGDRKATKGQVLLDGLAPQARSNKETVAVLPQENAIPTDLKVKELLRFFQAIYKD
                     SLTDVEIDSLLCFSQEQKNQLAGKLSGGQKRLLAFVICLIGKPKLLFLDEPTAGMDTS
                     TRQRFWEIVHELKAQGVTIFYTSHYIEEVEHTAERILVLHQGRLLRDTTPFAMRSEEQ
                     EKEVTLPIAFAAVVESLAEINGISYKQDTVSFKTRAIEQVWASLQEAGCRISDLEVQN
                     KTLLNSLFDKTREEES"
     gene            1749..2480
                     /locus_tag="AC1PIW_000840"
     CDS             1749..2480
                     /locus_tag="AC1PIW_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6605796.1"
                     /translation="MKALVQVEAVKLSRSLGVFLLSIGMPVIFFLIFSSTVRFDDAAM
                     QKAFIQSYMLTMTGFSMSGFALFTFPMMLAEDRKNSWLTFIQHSPLPIWHYYLSKLVR
                     VLLCFVSSIAVVFAVGGLVKGVDLSAQEWVVSALILLVTSIVFLAIGLLLVQIQSEQA
                     MSVVANLLYFVLAIMGGSWMPVSLFPDWVQRICKLMPSYHVNQLVTTYAQEGDFLWKS
                     LLIVLGYAIIFLGIALVLNRKSEQQ"
     gene            2489..3583
                     /locus_tag="AC1PIW_000841"
     CDS             2489..3583
                     /locus_tag="AC1PIW_000841"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIP6605797.1"
                     /translation="MIFEKRKVPLMFFVGMVFLYFPFYYAQYSPNPTAVVLATILFAL
                     VYCSLFYTDHKLYSLLAWFYLIGYIAVMSFWGNLQFSLFIFNLSNMLGWYYKETRPTY
                     RTVSSGILLLAIILWALFGPVPFEMRAFALVIHFFGLALLIFSWIEIKREALQQRLQE
                     QNASINLLLAENERNRIGQDLHDTLGHVFAMLSVKAELVQTLLDHDQIDQAKKEVQEL
                     QTLAKDSMQDVRQIVQSLKQYTVAEELQILQQMLDLAGVDLLVLGGEIAEHLSLPVQN
                     KLAMVLRELGNNLLKHSQASRCRLVFEKDQQELVLYYEDNGVGFAKLTGQELHTIKDR
                     LVTVKGSLEFLSLANPTRLSIRIPFEEVVE"
     gene            3580..4182
                     /locus_tag="AC1PIW_000842"
     CDS             3580..4182
                     /locus_tag="AC1PIW_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIP6605798.1"
                     /translation="MRILVAEDQAMLRDALCQLLCFQDAVEAVLQAENGSQAIALLEK
                     EPVDVVLLDIEMPEKTGLDVLEWVRGQALPVKVVIMTTFKRPGYFERAVKADVDAYVL
                     KERSIADLMRTIETVLAGRKEYSPELMDILLTQRSPLTNLESQLLKLVAEGLSNKEIA
                     CQLHLSDGTVRNYMTSILSKLGAENRTAAVKVAQEKGWLV"
     gene            4490..4708
                     /locus_tag="AC1PIW_000843"
     CDS             4490..4708
                     /locus_tag="AC1PIW_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HIP6605799.1"
                     /translation="MKRLYDVQQLLKRFGIIVYMGNRLYDIEMMQIELNRIYQAGVLD
                     RLEYMEAELVLRREHRLELEYQKSRENE"
     gene            4708..5094
                     /locus_tag="AC1PIW_000844"
     CDS             4708..5094
                     /locus_tag="AC1PIW_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIP6605800.1"
                     /translation="METFWVFLVFLAVLGAWMGFNYWRLRRAATVIDNAEFAEKIHGG
                     QLIDLRDPSEFNRKHILGARNIPYQQLRQSTAALRKDRPVLIYENDRGQLVTPAALHL
                     KKQGFNEIYILSYGLNGWNGKVKTTK"
     gene            5151..5873
                     /locus_tag="AC1PIW_000845"
     CDS             5151..5873
                     /locus_tag="AC1PIW_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HIP6605801.1"
                     /translation="MEKKFEKLSLAEIGIISFMGLILLISLGASWYKNAQSETSDTKT
                     VQQVSNQKKTVDTLFQDAENAVIVLEGTHTEEKLAEAQSAVNKVTDATKKAALQKRID
                     QVKELVGQQTDAQTQAKLKAELVRAEAAQKVADEKKVSETGTSQSTDLAEASETSQES
                     YVVPNQNIYGNQSVSSTSQYTSSVASTSTSTSVPPTSEGAPVVETPTVSEAPAETTDS
                     TTSLVESVETVPTADESSPDNP"
     gene            6181..6720
                     /gene="rsmD"
                     /locus_tag="AC1PIW_000846"
     CDS             6181..6720
                     /gene="rsmD"
                     /locus_tag="AC1PIW_000846"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIP6605802.1"
                     /translation="MRIVSGSYGGRPLKTLDGKTTRPTSDKVRGAMFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAISRGMESAVLVERDRRAQAIIRENIAMTKEADKFQLLAMEARQ
                     ALNSVGGPFDLVFLDPPYAEQEIEDVITELCRRDLLADDVMVVCETDKAVSLPEEIAE
                     LGIWKEKLYGISKVTVYVR"
     gene            6710..7198
                     /gene="coaD"
                     /locus_tag="AC1PIW_000847"
     CDS             6710..7198
                     /gene="coaD"
                     /locus_tag="AC1PIW_000847"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIP6605803.1"
                     /translation="MSDKIGLFTGSFDPITNGHLDLIERASGLFDKLYVGVFTNPKKA
                     GLLTGLERKAILEKLFVGMENIEVVLSENELVVDVAKRYGVTHLVRGLRNATDLEYES
                     SFDFYNRQLAPGLETIYLIAKPELKFVSSSQVRELLYFKQDIGPYVPEIVSEEIRKNE
                     EK"
     gene            7185..8234
                     /locus_tag="AC1PIW_000848"
     CDS             7185..8234
                     /locus_tag="AC1PIW_000848"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HIP6605804.1"
                     /translation="MKKNRKLTLIASIVLGTLLIWFAVFVRLPYYLESPGGAADIRQV
                     LTVNNQIDQEDGSYNFTYVSVQQATALQLFAAQFDPYTTVRSSEEMTGGADNEEYFRI
                     ARFYMETSQNMAKYQGLTLAGKEVNLDFFGVYVLALTDDSTFKKVLNIADTVVSINGK
                     TFESSPDLIKYVSGLEVGSDVTVGYISAGQEKSADGKIIKLSNGKNGIGITLVDHTEV
                     QSSIPIDFQTGDIGGPSAGLMFTLAIYTQLAEPDLRDGRIIAGTGTIEQDGKVGDIGG
                     ADKKVISAAKSGASIFFVPNNPVDVEVLKKNPKAKTNYEEAKEAAEKAGLDIEVVPVK
                     TVQEAIDYLKKTKGE"
     gene            8422..9063
                     /locus_tag="AC1PIW_000849"
     CDS             8422..9063
                     /locus_tag="AC1PIW_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HIP6605805.1"
                     /translation="MKKEISPEMYNYNKYPGPSFARVGDKVVSENIELDLLEDYKNAF
                     DQTIFGQRFSQLMLKFDYIVGDWGNEQLRLRGFYTDDKNVKSDLKISRLDDYLTEYCN
                     FGCAYFVLANPNPQDLPAEEENRPRRKRSRSRNRNRNQPRTEPIAPKENKSNRNDRQK
                     KGKQQNKQSEQRHFTMKNAGVKASQTERSQKRERKQSKREMNEVKRNFVIRQK"
     gene            9072..10184
                     /gene="rlmN"
                     /locus_tag="AC1PIW_000850"
     CDS             9072..10184
                     /gene="rlmN"
                     /locus_tag="AC1PIW_000850"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HIP6605806.1"
                     /translation="MKPSIYAFSQANLVDWILENGEKKFRATQIWEWLYRSRVQSFAE
                     MTNLPKSLIEKLEEHFVVNPLKQRIVQESKDGTIKYLFELPDGMLIETVLMHQHYGLS
                     VCVTTQVGCNIGCTFCASGLIPKQRDLTSGEIVAQIMLVQKYLDERNQNERVSHIVVM
                     GIGEPLDNYDNVMTFLRVVNDDKGLAIGARHITVSTSGLAPKIREFAREGVQVNLAVS
                     LHAPNNDLRSSIMRINRKFPIEVLFEAIEDYIKVTNRRVTFEYIMLNEVNDGVEQAQE
                     LADLTKNIRKLSYINLIPYNPVSEHDQYSRSTKERTLAFFDVLKKNGVNCVVRQEHGT
                     DIDAACGQLRSNTLKKDREKARARIAAAKAKAGIRA"
     gene            10181..10708
                     /locus_tag="AC1PIW_000851"
     CDS             10181..10708
                     /locus_tag="AC1PIW_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIP6605807.1"
                     /translation="MRKFFQADGNLTKLGRQICKTLAGAYALAIVLLCFLPQTWYPQY
                     KDFSTPGIIQIGRLYLLPTPFNSIVNGDKVDSLADLGWIFLQNITNIFLLFPLIFLLL
                     FLREEWRSLRAVIRYSFCISLFIECTQLLLDLLIDAQRVFEIDDLWTNTMGGILAYFS
                     YSLIKRGIILSKQES"
     gene            complement(12004..12828)
                     /gene="nadE"
                     /locus_tag="AC1PIW_000852"
     CDS             complement(12004..12828)
                     /gene="nadE"
                     /locus_tag="AC1PIW_000852"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HIP6605808.1"
                     /translation="MTLQETIIKELGVKPSIDPKEEIRRSIDFLKDYLKKHPFLKTYV
                     LGISGGQDSTLAGRLAQLTMEEMRAETGDDSYQFIAIRLPYGVQADESDAQAALAFIQ
                     PDVSLTINIKESTDAMVAAVNANGLAMSDFNKGNAKARMRMIAQYAIAGERKGAVIGT
                     DHAAENITGFFTKHGDGGADILPLFRLNKRQGKQLLAALGADEKLYLKVPTADLEEDK
                     PGLADEVALGVTYNQIDDYLEGKTIDPQSQTIIEGWWNKTAHKRHLPITIFDDFWK"
     gene            complement(12841..14301)
                     /locus_tag="AC1PIW_000853"
     CDS             complement(12841..14301)
                     /locus_tag="AC1PIW_000853"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HIP6605809.1"
                     /translation="MTLFKDDSLTLHTDLYQINMMQVYFDQNIHNKHAVFEVYFRSQP
                     FKNGYAVFAGLERVVNYLNNLSFSQTDIDYLTELGYPKDFLDYLSQLEMTLTVRSAKE
                     GDLVYANEPILQIEGPLAQCQLIETALLNIVNYQTLIATKARRIKAVIEDEPLMEFGT
                     RRAQEMDAAIWGTRAAFIGGASATSNVRAGKIFGIPVAGTHAHALVQTYGDDYKAFKA
                     YAETHHDCVFLVDTYDTLRIGVPAAIKVAKEMGDKINFKGVRIDSGDMAYISKKVRQQ
                     LDDAGFTDAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVS
                     IEDEDGNMRDTIKLSSNAEKVSTPGKKQVWRITSKEKGKSEGDYITFADTDVTQMDNI
                     YMFHPTYTYINKTIENFDARPLLVPIFEKGKQVYELPSLAEIQTYANQEFDKLWDEYK
                     RVLNPQLYPVDLAQDVWDNKMNLINRIRKQTQTKSI"
     gene            complement(14409..15053)
                     /locus_tag="AC1PIW_000854"
     CDS             complement(14409..15053)
                     /locus_tag="AC1PIW_000854"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HIP6605810.1"
                     /translation="MKTIILFFLGASIGSFLGLVIDRFPEQSIIAPSSHCNACKRRLK
                     AWDLIPVLSQLSTKSKCRYCKAKIPYWYLGLEFLAGLVVLLCHFQVLNLTETILILAG
                     LVLTIYDIKHQEYPFAVWLIFTFIALILSQLNWLFCGFLLLAYLTEKWQINIGSGDFL
                     YLASLALICGFTELLWIIQISSLLGLLVFAIFKPKSIPYVPLLFLSSIPIILCT"
     gene            15175..15729
                     /locus_tag="AC1PIW_000855"
     CDS             15175..15729
                     /locus_tag="AC1PIW_000855"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (mnm(5)s(2)U34)-methyltransferase"
                     /protein_id="HIP6605811.1"
                     /translation="MLRPLHMAHAFLDEILTDQDLAVDATMGNGHDTLFLAQRAGKVV
                     AFDIQEQALTTTAEKLEKAGLTNAQLVLTGHENLDQYVDECKAAIFNLGYLPSADKSV
                     ITLPATTLQAIEKVLNRLVVGGRLAIMIYYGHEGGALEKDAVLDFISQLDQTVFTAML
                     YKPLNQVNTPPFLVMVERLKSSSN"
     gene            complement(15745..16524)
                     /locus_tag="AC1PIW_000856"
     CDS             complement(15745..16524)
                     /locus_tag="AC1PIW_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6605812.1"
                     /translation="MIISMKNVHYKRQGKTILEDINWEFRKGERWAILGLNGAGKSTL
                     LRILMAEFWKTSGDLTVLGVEFGKGDIPSLRTKIGVVGSFLAERFPIDLTAEQIVLTG
                     KYKSSILYREYGQAELDEAIDMLKTIKAEHLIGRAYASLSQGERQLLLIARSLMEEPA
                     ILILDEATVGLDLLARERLLHHIDYLCQLPTAPAIIYVTHHAEEITDNFTHVLLLKDG
                     MVLAQGPKEDILTPELLSDFYGNQVELIDLGEGRLFIKPIL"
BASE COUNT         4677 a         3208 c         3943 g         4776 t
ORIGIN      
        1 aaacaaaaat attatcaatc tccagcagaa attgcgtctt tcagcccgcg tccatcactg
       61 gctgattcta aggctgtttt gaatcaagcg gtggccgatt tatcagtagc ccattcaatc
      121 ctccatcaag ttcactggta tatgcgtggt cgtggcttta tgatttggca tccaaagatg
      181 gatgaatata tggaagaaat tgatggttat ttggatgaga tgagtgagcg tttaatcacc
      241 ttaggtgggg caccattttc tacccttaaa gagtttagtg aaaatagtca gctcaaggaa
      301 gttcttggtg attacaatgt aacgattgaa gagcaattgg cgcgtgtggt agaggtgttc
      361 cgctatctgg ctgctctttt ccaaaagggg tttgatgtca gtgatgaaga aggtgacagc
      421 gtaaccaacg acattttcaa tgtggctaag gctagtattg aaaaacatat ttggatgctg
      481 caggcagaac ttggtcaagc acctaagttg taaatagatg aagggtccac agtaggactc
      541 tttttttgct acattattta atactcttcg aatatcaaaa ttatccgttg tcaacttgcc
      601 ttgatgaact ccagatctat cttcgacttc gtttcctagg ctacttttga ttttcattga
      661 gtataatatg acatttgtca catgagtcca tgacaaaggc ttctagtggg gtctcctttt
      721 tcgagatata ctgtctatgt atcgtcattt agtttttctt ttattacttc gttaactcgc
      781 cttgcctaat tccagttatg cctgcggctc gttgcctagt actaaaagta aactaaaaga
      841 ctataagatg ttcaagaagt aggaggtaga aaaatgatag aagtacaagc actagaaaaa
      901 atcatcaaag gacggacgat tttgtccact atttcctttg aaatcaagtc aggagaatgt
      961 gtggctttaa tcggtccaaa cggggctgga aaaacgacgc ttatgtcttg tttgttgggt
     1021 gatagaaagg cgacgaaagg ccaggttttg ttggacggtc tggctccaca ggctcggtcc
     1081 aataaagaaa cggtagcagt cttaccacaa gaaaatgcta tcccgacaga cctgaaggtc
     1141 aaggaattac tccgcttttt ccaagctatt tacaaggata gtctgacaga tgtggaaatc
     1201 gacagcctgc tctgcttttc ccaagagcaa aaaaatcaac tggccggcaa gctatcgggt
     1261 ggtcagaagc ggctcttggc tttcgtcatc tgcctaattg gcaagcccaa gttgctcttc
     1321 ttggacgagc cgacggcggg catggatact tcgacccgcc agcgtttttg ggagattgtc
     1381 catgaactta aggctcaggg tgtgactatt ttttatacca gccactatat tgaggaagtg
     1441 gagcatacgg cggagcggat tttggtcttg catcaggggc ggctactaag agataccacg
     1501 ccatttgcta tgcggagtga ggagcaagaa aaagaagtga ctttgccgat tgcctttgcg
     1561 gcggtggtgg agagcttggc tgaaatcaat gggatcagct acaaacaaga tacggtcagc
     1621 ttcaagaccc gagctattga gcaggtttgg gctagtttgc aggaggcagg ttgtcggatt
     1681 tcagacctgg aagtgcagaa taaaactctg ctcaatagcc tatttgacaa gacgagggag
     1741 gaagaatcat gaaagcatta gttcaagtag aggcggtaaa attatccaga agtttgggag
     1801 tatttctcct gtctatcggt atgcccgtca tctttttcct gattttctca tcaacggtcc
     1861 ggtttgatga tgcagccatg caaaaggcct ttatccagtc ctacatgctg acaatgacag
     1921 gtttttccat gtcgggcttt gcccttttca cctttcccat gatgttggca gaggacagaa
     1981 agaatagctg gttgaccttt atccagcatt cacccctgcc aatctggcac tactacctat
     2041 ccaaactggt acgggtcttg ctctgctttg tatcttccat tgccgttgtc tttgcggtgg
     2101 gtggcttggt caagggagta gatttgtcag ctcaggaatg ggtcgtgtcc gcactcatac
     2161 tccttgtaac ttctatcgta tttctagcta tcggcttgct cttggtgcaa atccagtctg
     2221 aacaggccat gtcggttgtg gcaaacttgc tctactttgt tctggctatc atgggtggct
     2281 cttggatgcc tgtttccctc tttccagact gggtgcagcg gatttgtaaa cttatgccta
     2341 gctaccatgt caaccagctg gtgaccacct atgcccaaga gggagatttt ctctggaaat
     2401 ccttgctaat tgtcctaggc tatgcgataa ttttcttagg aattgctcta gttctgaata
     2461 gaaaatccga gcaacagtga ggtaagccgt gatttttgaa aaaagaaagg tgccgctcat
     2521 gttcttcgtg ggcatggtct ttctctattt tcccttttac tatgcccagt atagtcccaa
     2581 tccaacagca gttgtcctag cgactattct ctttgccttg gtttattgct ccctctttta
     2641 taccgaccac aagctctatt ccctgctggc ttggttttac ctgattggct atatcgccgt
     2701 catgagtttt tggggcaatc tgcaattttc cctcttcatc tttaacctgt ccaatatgct
     2761 aggctggtac tacaaggaaa ctcggccgac ctatcggacg gtgtcctctg ggatcttgtt
     2821 actggccatt atcctctggg ctctctttgg ccctgttcct tttgagatga gagcttttgc
     2881 acttgttatc cacttctttg gcttggcctt gctgattttc agctggattg agatcaagcg
     2941 ggaagccctt cagcaacgcc tacaggagca gaatgcgtcc atcaatctcc tgctggctga
     3001 gaatgaacgc aaccgtatcg gtcaggactt gcacgatacg ctggggcatg tctttgccat
     3061 gctgtctgtc aaggctgagc tggtccagac acttttagac catgatcaga ttgaccaggc
     3121 caagaaggaa gtgcaggagc tccagacctt agccaaagac tccatgcagg atgttcgcca
     3181 gattgtccag tccctcaagc agtacaccgt ggcagaggaa ctccagattt tacagcagat
     3241 gttggacctg gcaggagtgg atttgcttgt cttgggcggg gaaatagcag agcatctcag
     3301 cttacctgtc cagaacaagc tggctatggt gctgagagag ttgggcaata acctgctcaa
     3361 gcacagtcag gctagcagat gccggttggt ttttgaaaaa gaccagcaag aattggtttt
     3421 atactacgaa gacaatggtg tgggctttgc caagttgact ggtcaggaat tacataccat
     3481 aaaagacagg ttggtgacgg tgaaaggcag tttggagttt ctttctctgg caaatcccac
     3541 ccgcctgtcc atccgaattc cgtttgagga ggtggtagaa tgagaatctt agtcgcagaa
     3601 gaccaagcaa tgctgcggga cgccctctgt cagctcttgt gttttcaaga tgcggttgag
     3661 gctgttttac aggcagaaaa cggcagccag gccattgctc ttttggaaaa agagccagta
     3721 gatgtggtgt tattagatat agaaatgcct gaaaagacgg gcctagatgt cttggaatgg
     3781 gtgcggggtc aagccttgcc tgtcaaggtt gtcatcatga ccaccttcaa gcgaccgggc
     3841 tatttcgagc gagcggtcaa ggcagatgtg gatgcctatg tgctcaagga acgctccatc
     3901 gcagacctca tgcggaccat tgagacggtc ttggcagggc ggaaggagta ttcgccagag
     3961 ttgatggata ttctcttaac ccaacgcagc cctttgacca acctagaaag tcaactactc
     4021 aagctggtgg cagaggggct gtccaataag gaaattgcct gccagctcca cctgtctgac
     4081 gggacagtcc gcaactacat gacctctatc ctgtccaagc taggcgccga aaaccgcacc
     4141 gccgctgtta aggtggcaca ggaaaagggt tggttagtat aaacgaatga gattgggcat
     4201 ttgtccaatc tttttataga ccggattttt gactgagttt ttatactctt cgaaaatcaa
     4261 aatcagacgt tgttgacttg atttgatgag taaaaggctc cagtggagcc tttcagcctg
     4321 ttcccttgaa acaaaatagt aacagggttc tatctgcgta agtagtaacg aactacgttc
     4381 gctctatctc caacctctaa aggttcccca aacctttgga gctagtctgt ttttgatttt
     4441 cattgagtat tatattgaaa aacacctaaa attctgctac aattttttta tgaaaagact
     4501 ttatgatgtg caacaattac tcaaacggtt tggtataatt gtctatatgg gcaaccgtct
     4561 ctacgatatt gaaatgatgc agatagaatt aaatagaatc tatcaggcag gtgtcttaga
     4621 tcggttggaa tatatggaag cagagcttgt tttgaggcgt gaacatcgtt tggaattaga
     4681 gtatcagaaa tcaagggaga atgagtgatg gaaacatttt gggtattttt agtatttttg
     4741 gcagtattgg gagcctggat gggctttaat tactggagat tgcgtcgtgc agcgactgtg
     4801 attgataatg cagaatttgc agaaaagata cacggaggtc agttgattga cctgcgagac
     4861 ccgagtgagt ttaatcgtaa gcatatctta ggtgcgagaa atattcctta ccagcaattg
     4921 cgccagtcta cagctgcttt gcgtaaggac agacctgtct taatctatga gaatgatcgt
     4981 ggtcagttgg taacgcctgc agcccttcat ctgaagaaac agggctttaa cgaaatctat
     5041 attttaagct atggtctaaa cggatggaat ggtaaggtaa aaacaactaa gtagcattgt
     5101 cagtaggcta gagtattttc ttatgcaata agacggataa gggaaaaaat atggaaaaga
     5161 aatttgaaaa gttgtcattg gcagaaattg gcattatttc ttttatggga cttattttat
     5221 tgattagctt aggcgctagt tggtataaaa atgctcaaag cgaaacaagt gatacaaaga
     5281 cagtacagca agtgtccaat cagaaaaaaa cggttgatac attatttcag gatgctgaga
     5341 atgctgtcat tgtattagaa ggaacacata ctgaagagaa acttgcagag gcacaatctg
     5401 cagtaaacaa ggttacggat gcgactaaaa aagcagcatt acagaaacgg attgaccaag
     5461 taaaagagct agtgggacag cagacagatg ctcaaacaca ggctaagcta aaagcggagt
     5521 tggtcagagc ggaagcagcc caaaaagtag ctgatgagaa aaaagtatct gaaacaggga
     5581 ctagtcagtc aacggacctt gctgaagcta gtgagacctc acaagaatcc tatgtagtgc
     5641 ctaatcaaaa tatatatgga aatcaaagtg tgagttcaac aagccagtat acaagcagcg
     5701 tggcgtcaac tagtacaagt acttcagtgc ctccgactag tgaaggagca cctgtggtag
     5761 aaactccaac agtttctgaa gccccggcgg aaacgacgga ttcaacaact agcctggttg
     5821 agtcagtaga aacagttcca acagctgacg agagttcacc agataatccg tagacttttt
     5881 agctactggt tctttatggc gtattagcat actggaaaaa atagctattt gtatttcaac
     5941 atcaattttt tcttgtaacg atctagttta aatacatacg ataagagtga ggaatttcgc
     6001 gtaaaacaga atttccttgc tttttatttt tatcattttg ttgaatagtg cgagaataat
     6061 ttcctgctat agcggttgag agtgtatttg gtagaaggtt gaaaaagttg gtgcaaaaag
     6121 gaaagcagga agtgcacttg gtttcagcaa cgagcttcat tttttgctat aatatacctt
     6181 atgagaattg tatcgggaag ctatggtgga agaccactga aaacgttaga tggaaaaaca
     6241 accagaccga catcggataa ggtccgtgga gccatgttca atatgattgg tccctatttt
     6301 gagggtggta gagttcttga cttgtacgcc ggcagtggtg gtttatctat cgaggctata
     6361 tctcgtggca tggagtcagc agttctggtt gagcgagatc gacgagcaca agccattatc
     6421 cgtgaaaata ttgcaatgac taaggaagca gataaatttc agcttctagc aatggaagct
     6481 agacaggctt tgaacagtgt gggaggtcct ttcgacctgg tattcttgga ccctccttat
     6541 gcagagcagg aaattgaaga tgtgataaca gaactttgtc ggcgtgactt actagcagat
     6601 gatgttatgg tagtctgcga aacggataag gcggtctctc ttccggagga aatagcagag
     6661 ctggggatat ggaaagagaa actctacgga attagtaagg taacagtata tgtcagataa
     6721 gattggattg tttacgggct catttgaccc cattaccaat ggacacttgg acttaattga
     6781 acgagcgagt ggactatttg ataaattata tgtgggagtt tttaccaatc ctaaaaaggc
     6841 aggactcttg acaggtttgg agagaaaagc cattttggag aagctttttg taggaatgga
     6901 gaatatcgag gtcgttctgt ctgaaaatga attggtagtc gatgtagcta agcgctatgg
     6961 agtaacacat ttggtccgag gattgcgtaa tgcgaccgat ttggaatacg agtcaagttt
     7021 tgatttttac aatcgtcagt tggcgccagg tttggaaact atttatttaa tcgccaagcc
     7081 agagcttaag ttcgtatcat ccagccaggt tagagaattg ctgtatttca aacaggatat
     7141 aggcccttat gttccagaaa ttgttagtga ggagattaga aagaatgaag aaaaatagga
     7201 agttaacctt aattgcatct attgttttag gaactctttt gatttggttt gccgtttttg
     7261 ttcgtctgcc ttattatctg gaaagtccag gtggagctgc cgatattcgt caggtactaa
     7321 cggtgaacaa tcaaatagac caagaggatg gctcttacaa cttcacctat gtatcagtcc
     7381 aacaggctac agctttacaa ctattcgctg ctcagtttga tccctatacg acagttcgtt
     7441 cctctgaaga gatgacagga ggggccgaca atgaggagta tttccgtata gcacggtttt
     7501 acatggagac atcgcaaaat atggcgaagt accaaggttt gaccttggca ggaaaagaag
     7561 tcaatcttga tttctttggt gtctatgtgc ttgccttgac ggatgattca acctttaaaa
     7621 aagttcttaa tattgccgac actgttgtga gtatcaatgg aaaaacattt gagtcttctc
     7681 ctgatttgat taaatatgtg agtggcttgg aggttggaag tgatgtaaca gttggttata
     7741 ttagcgctgg tcaggaaaaa tctgcggacg gtaagattat caaattgtct aatggcaaaa
     7801 acgggatagg tatcactctc gtagatcata cagaggttca aagttcgatt ccgattgatt
     7861 tccaaacagg agatattggt ggtccaagtg ctggtctgat gtttacactt gccatctata
     7921 ctcaactcgc agagcctgac ttacgtgatg gtcggattat tgctggtacg gggactatcg
     7981 agcaggatgg aaaagtaggt gacattggtg gggctgataa gaaggtcatt tcggcagcta
     8041 agtctggtgc aagtatcttt tttgtaccaa acaatccagt tgatgtagaa gttttgaaga
     8101 aaaatccaaa ggctaaaacg aactatgaag aagctaagga agcagccgag aaagcaggtc
     8161 tagatattga ggtagttcct gtgaaaactg ttcaagaggc tattgattat ttgaagaaaa
     8221 caaagggcga gtaaatcgtc cttttttgct agagaataat attataattg tcagaaattt
     8281 ctcctaaatg gtaaagtaga agaatttcca gataaatcgt gttataatga taaggttaag
     8341 tgtgagaatt tagattctga ccagatagat tattaatcaa atgattttta cgtatgaaga
     8401 ttttataaga taggaggaca aatgaaaaaa gaaatttcac ctgaaatgta taactataat
     8461 aaataccctg gtccgagttt tgctcgtgtc ggagataagg ttgtatctga aaatattgaa
     8521 ttggaccttt tggaagatta caagaatgct tttgatcaga cgatttttgg tcaacgtttt
     8581 tcacaactca tgctcaaatt tgattatatt gtgggagatt ggggcaatga gcagctgaga
     8641 ttaaggggat tctatacaga cgataaaaat gttaaatcag acttaaaaat cagccgctta
     8701 gacgactatt tgacggagta ttgtaatttt ggttgtgctt atttcgtctt agccaatccg
     8761 aatccacaag acttgcctgc tgaggaggag aatagaccgc gtcggaagcg gagtcgttct
     8821 cgcaatcgga acagaaatca gccgcggacg gagcctattg cgccaaaaga aaacaaaagt
     8881 aatcgcaatg accgtcagaa aaaggggaag cagcaaaaca aacagtcgga acagcgtcat
     8941 tttactatga agaatgctgg tgttaaggcg agtcaaacgg agcgttctca aaaacgtgag
     9001 cgtaaacaaa gcaagcgtga gatgaatgaa gtgaagcgta actttgttat ccgccagaaa
     9061 tagggagaaa tatgaaacca tcaatttatg catttagtca agccaatctg gttgattgga
     9121 ttttagaaaa cggagagaag aagttccgtg ccactcaaat ttgggaatgg ctctaccgtt
     9181 ctcgtgtcca atcttttgcg gaaatgacca acttgccaaa atctttgatt gaaaagttgg
     9241 aagaacattt tgtagttaat ccgctcaaac aacggattgt acaggagtct aaggacggta
     9301 ctatcaaata cctttttgaa ctgccagatg gcatgttgat tgagacagtt ctcatgcacc
     9361 agcactatgg cctttctgtt tgtgtaacga cacaagttgg ctgtaatatc ggctgtacat
     9421 tctgtgcatc tggattgatt cctaagcaac gtgacttgac tagcggtgag attgtggctc
     9481 agattatgtt ggtacaaaag tacttggatg agcgcaatca gaatgagcgt gttagccata
     9541 tcgttgtgat ggggattggt gagccgcttg ataactacga taatgttatg accttcttgc
     9601 gagtggttaa tgacgacaag ggcttggcaa ttggtgcccg tcacatcact gtttctacgt
     9661 caggtttagc accgaaaatt cgagagtttg cacgtgaagg tgtccaagta aacttggcag
     9721 tatctctaca tgcgccaaat aatgaccttc gttcgagcat tatgcgcatt aatcgcaaat
     9781 tccctataga agttcttttt gaagcaatcg aagactatat caaagtgacc aaccgccgtg
     9841 tgacttttga gtatattatg ctcaatgagg tcaacgatgg agtggaacag gcacaggaat
     9901 tggctgactt gactaagaat atccgtaaat tgtcttatat caacttgatt ccttataacc
     9961 cagttagtga acatgaccag tacagtcgtt caaccaagga acgtactctt gctttctttg
    10021 atgttttgaa gaaaaatggt gttaactgtg tcgttcgtca ggaacatggt acagatattg
    10081 atgctgcctg tggtcaattg cgctccaata cgctcaagaa agaccgtgaa aaagcccgtg
    10141 ctcgtattgc agccgcaaaa gcaaaggcag gtattcgagc atgagaaaat tcttccaagc
    10201 agatgggaac ctgaccaagc tgggaagaca gatttgtaag accttagctg gagcctatgc
    10261 tctggctatt gttttacttt gtttcctacc acagacttgg tacccacagt ataaggattt
    10321 ctcgacccca ggaattattc aaattggccg tctctatctt ttaccaactc ctttcaacag
    10381 catcgtcaac ggggacaagg tagatagttt ggcggaccta gggtggattt tcttgcaaaa
    10441 catcactaac atttttctac ttttccctct gatttttctt cttttgtttc ttagggaaga
    10501 atggcggagc cttcgagcgg tgattcgtta tagtttttgt atcagtcttt ttattgaatg
    10561 cacacaatta ctactggatt tgctgattga tgcccaacgc gtctttgaaa tcgatgattt
    10621 atggaccaat acaatggggg gaatactagc ctattttagc tattcattga taaagagagg
    10681 gataatcctt tccaaacagg agtcttagaa cctaccgtca caggttcaga atgtccatct
    10741 aatgctattg tttaagctac atatcgttat ttttttgaaa tacagtcagt attcccttaa
    10801 aaatgacctt gactagctaa agctatcttt gatatgggag tgggaaagac atttaggttc
    10861 ggcttctatg gtcactttct agttttaagg tgaccagctg aaacagttcc tcaaattgtt
    10921 tcactcccac ctccgcatag ctccgaaggt tcggggaacc tttggaggct ggagatatag
    10981 cgaacaaagt tcgcatcagt cgtagaggtc agatttggag tgtaaaacat gaattgctga
    11041 gatttgcttc gcaaatctta tctccaacct ttaacctcgc ttttcccttt ctaggatcgg
    11101 gctgaaacag ttccccgaac tgtttcactc cactggactg ttaaacccaa tcaaccactg
    11161 cgctgagatg gtgacactaa ctttgagaag cggtgctggg cttgagccca gcctcgtgag
    11221 tacagttttt tagaaagtca cttaagcaga gtggcttttt gttagttttt aatggattca
    11281 ggcaatggaa actgaatttt caaattgttt agaaaatttc taaaaatcac ttgaccattt
    11341 tgattttgta acgtataatt acaagtaatc agaaaatgta ataaacactt tgtctttaga
    11401 gagtctatgg ttggtgtgaa tagatgatag aggtttatga gataggctga tgccaaacaa
    11461 gcattcgtat ccattttatg gattgtctgg tttagtaaag tgagtttggt tcgatgaatt
    11521 cgaaacaatg agagttcggt ccgcacccct tatcgtgcgc ctccttgtta gaggagaaaa
    11581 agttatggac ttttctagtc gagtccatag tgaggtggca ccgtgtccat tgacgcccac
    11641 gcaggatatt ttttcctgcg tgggcttttt tcattatggt ttgaagttat aataaaaaac
    11701 aaaacccctg acggttcaac tgtcagggct ccatttttta ttattcgatg aaaatcaaaa
    11761 gtagcctagc ttcaactatc tgggagatag ttgaaggttg gaaatatagc gaacatgttt
    11821 cgcatcaaaa ctatctggga gatagttgaa gctaggaaat atagcgaaca ttgtttgcgc
    11881 cttcaagttg atgttagaac tctgttcctt ttatatttca agggaacagg ctgaaaacct
    11941 ccacaggagc ttttcagtca tcaaatcaag tcaataacgt ctgattttga ttttcgagga
    12001 gtattatttc caaaaatcat caaaaatagt aattggcagg tggcgtttat gggctgtttt
    12061 gttccaccat ccttcgataa tggtttggga ttgagggtcg atggttttac cttcgagata
    12121 gtcgtcgatt tggttatagg taacaccaag tgcgacttcg tcagcaaggc ctggtttgtc
    12181 ttcttccaag tctgcagtag gaactttgag gtagagtttt tcatctgctc ccaaagcagc
    12241 tagaagttgc ttaccttgac gcttgttaag gcggaagagg ggcaggatat ctgcacctcc
    12301 atcaccgtgc ttggtaaaga aaccagtgat attttcagcg gcatgatcgg ttccaataac
    12361 agcccccttg cgttctccag cgatagcgta ttgggcaatc atgcgcatgc gggctttagc
    12421 atttccctta ttaaaatcag acatagcaag cccatttgca ttgactgcag ccaccatggc
    12481 atctgttgat tccttgatgt tgatggttaa gctgacatct ggttgaataa aggctagggc
    12541 tgcttgggcg tcagactcat cagcttgcac accgtaaggc aggcggatag cgataaattg
    12601 ataagaatcg tcacctgttt ccgcccgcat ttcttccata gtcagctggg ccaaacgacc
    12661 agccaaggtc gaatcttgtc caccagaaat ccccaagacg taggttttca aaaatgggtg
    12721 ttttttcaga tagtctttga gaaagtcgat ggagcgacgg atttcttctt tggggtcaat
    12781 gcttggcttg acgcctaatt ctttgatgat tgtttcctgt agggtcataa catacctttc
    12841 ttagatggat ttggtctgag tttgtttgcg aatacgattg atgaggttca tcttattatc
    12901 ccaaacatct tgtgccaagt cgactggata gagctgtggg ttgagcacgc gtttgtattc
    12961 atcccagagt ttatcaaatt cttgattggc ataggtttga atttccgcta ggcttggaag
    13021 ttcatagacc tgttttcctt tttcgaaaat aggaactagg agaggacggg catcaaagtt
    13081 ctcgatggtt ttattgatat aggtgtaagt tggatggaac atatagatat tatccatctg
    13141 tgtgacgtca gtgtccgcaa aggtgatgta gtccccttct gattttcctt tttctttgga
    13201 ggtgatgcgc caaacctgtt tcttaccagg agtggagact ttttccgcat tagaagacag
    13261 cttgatggtg tcacgcatat ttccatcttc atcttcgatg gatacaatct tgtagacagc
    13321 gcccagagct ggttggtcgt aggcggtgat gagtttggta ccaactcccc agacatcaat
    13381 ttttgccttt tgcattttga ggttgaggat ggtattttca tcgaggtcat tagatgcgta
    13441 aatcttcgca tctgtaaaac ctgcatcatc taattgctga cgaactttct tagaaatata
    13501 ggccatatcg cctgaatcaa tccgaacccc tttgaagtta atcttatctc ccatttcttt
    13561 ggcaaccttg atggctgctg gaacaccaat acgaagcgta tcataggtat caacgaggaa
    13621 gacacagtca tgatgggttt cagcgtaggc tttgaaagcc ttgtaatcat ctccgtatgt
    13681 ttgaaccaag gcgtgggcat gggtaccagc gacagggatg ccgaagattt tgccagctcg
    13741 aacattggaa gtcgcagatg ctccaccaat aaaggctgcc cgtgtacccc agatagcagc
    13801 atccatttct tgggcacggc gagtgccaaa ttccatcaag ggctcatctt caatgacagc
    13861 cttgatacgg cgagcttttg tggcaattag ggtttggtag ttgacaatgt ttaagagggc
    13921 agtttcgatt agttggcatt gtgccaatgg tccttcgatt tgcagaatgg gttcgttagc
    13981 gtagaccagg tcgccctcct tagccgaacg gacagtgaga gtcatttcca attgggacaa
    14041 gtaatcaagg aaatccttcg gataacctag ttctgtcaga tagtcaatgt ctgtctggct
    14101 aaagctgaga ttgttgaggt agtttacaac gcgttccaaa ccggcaaata cagcgtagcc
    14161 atttttaaaa ggttgtgaac ggaaatagac ttcgaataca gcgtgtttat tgtggatgtt
    14221 ttggtcaaaa tagacctgca tcatgttgat ttgataaagg tcggtatgca gggttaaact
    14281 gtcatcctta aatagtgtca taacgtttct ccttttgcct tcctatcaag aaggtgctcc
    14341 aattattttg atggagttga actctaatat gccttctatt ataacaaaaa aatcgagaaa
    14401 aactcgattt aggtgcacag aatgatagga atacttgaaa gaaataggag tggtacgtag
    14461 ggaatagact tgggtttgaa aatggcgaag acaagaagcc ctaggaggga actaatctgg
    14521 ataatccaga ggagttctgt aaatccacat attaaggcca aacttgccag atagagaaag
    14581 tcaccagaac caatattgat ttgccatttt tcagtcagat aggccaagag taaaaagcca
    14641 cagaaaagcc agttgagctg ggagagtatc agagctataa aagtaaaaat gagccagaca
    14701 gcaaaaggat attcctgatg cttgatgtcg taaatggtca aaactagtcc tgccaagatg
    14761 agaatggttt cggttaggtt taggacttga aaatggcaga gcaggacaac tagaccggct
    14821 aagaattcca atcccagata ccaataaggt atcttcgcct tgcagtaacg gcatttggat
    14881 tttgtcgaaa gctgggatag gactggaatt aagtcccagg ccttgagccg tcgtttgcag
    14941 gcattgcagt gactagaggg ggcgataatg gactgttcag ggaaacggtc gatgaccaat
    15001 cccaagaagg agccgataga agctccaagg aaaaatagga taattgtctt catacctatt
    15061 tattcgcaaa aggaatagga aaaatggaag atttttaaaa aattttttga agatggaaag
    15121 tatgctatac tagatgtgat agattttatg tattgacgat aattaggaga aagtatgctt
    15181 agacctttac acatggccca tgccttttta gatgaaatat taacagacca ggacctagcg
    15241 gtcgatgcga ctatgggaaa tggccatgac acgctatttt tagcccaacg agcaggaaaa
    15301 gttgtggcct ttgatattca ggaacaagcc ctgactacaa cggctgaaaa actagaaaaa
    15361 gctggcttga ccaatgccca attggttttg acaggacacg aaaacctaga ccagtatgtg
    15421 gatgaatgca aggcagcgat tttcaatctg ggttatctac cttcggcgga caagtcagtc
    15481 attaccctgc ctgcaaccac ccttcaagcc attgagaaag tcctgaatag attggtggtt
    15541 ggaggtcgct tggctatcat gatttactat ggtcacgagg gtggagctct tgagaaggat
    15601 gcagttttag actttatcag ccagcttgac caaaccgtct ttacagccat gctctacaaa
    15661 ccgctcaacc aagttaatac cccaccattt ttggtcatgg tggaacgatt aaaatccagc
    15721 tcgaactaga gctggatttg ttttttataa tatcggctta atgaacaatc ttccctcacc
    15781 caagtcaatc aattccacct gatttccata aaaatcgctg aggagttcag gtgtcaggat
    15841 atcttctttg ggtccctgtg ccaagaccat gccatctttg agcaggagga catgggtgaa
    15901 attatcagtg atttcctcgg cgtgatgggt gacgtagata atggcaggag ccgttgggag
    15961 ttgacagagg tagtcaatgt ggtggagcag gcgctcgcga gctaggaggt ccaaaccgac
    16021 agttgcttcg tctaaaatca aaatagccgg ctcttccatc aaacttcggg caatcagcag
    16081 gagttggcgt tctccttgtg acaaactagc ataggcccga ccgatcaagt gctcagcctt
    16141 aatggttttg agcatgtcta tggcttcatc caattcagct tgaccgtatt cacgatagag
    16201 gatggaggat ttatattttc ctgtcaagac aatctgctca gccgtcaaat cgattggaaa
    16261 acgctctgcc aaaaaggagc caactacccc aatcttggtg cgtagactag gaatatctcc
    16321 cttaccaaat tccaccccca gaacagtcag gtcaccagag gtcttccaaa actcagccat
    16381 taaaatacga aggagggttg actttccagc cccattgagc cctaaaatag cccaacgctc
    16441 tccttttcta aattcccagt tgatgtcttc taaaatagtt ttaccttgtc gtttatagtg
    16501 gacattcttc atcgaaataa tcatagttgc tctcctattc cttttgatta ttgtaacata
    16561 aacagatatg agggactaat tataacaaga aatatgttaa tttt
//
LOCUS       DBNFCK010000033        16163 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.033, whole genome shotgun sequence.
ACCESSION   DBNFCK010000033 DBNFCK010000000
VERSION     DBNFCK010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 16163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16163
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.033"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(112..975)
                     /locus_tag="AC1PIW_000857"
     CDS             complement(112..975)
                     /locus_tag="AC1PIW_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIP6605813.1"
                     /translation="MSCFGKTLKFIREGKGKSLAEVAKGHISTSQLSRFENGESDITV
                     SKLFGVLAEMNVDVDEFVYASQDFKKDELTRILEQLKQSIYIRDVVTIKKILSEQLAK
                     AERDSRREFHRLNAILIAYKLRNLDKTIVVSKEDTAFLTDYLFRVENWSYYEILLFAN
                     TIDKLGHQTMMLFCRELIHRTQFYREMKQHRLVVSQVLLSAYLVSVQTKELVDAIFLE
                     KTIEGLLFDETEIFERILFKFGKAFYDYQNTGTSNAILEMRKCIGLLRAVDSQNIADW
                     YESILEKILIE"
     gene            complement(1465..3909)
                     /locus_tag="AC1PIW_000858"
     CDS             complement(1465..3909)
                     /locus_tag="AC1PIW_000858"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HIP6605814.1"
                     /translation="MKQIKLKATKQFILVMVMTVLLAMGRALLLSDVSFPLWFIPLPL
                     IAYLIFTFYVLVLLDKYEKFIKTKTFAKYVGYFLGFLYLINLVYRLNAKKYQPWNIFR
                     NNLFQFELLLMLALPVLLAFLWRKKTGIREKLSQWSANHLVPDGYLLLTSLLSLSPLA
                     ISYWKDSHYESLVEKGSYIEFFSQTPLFAPIHFIGTYIFLRYLHKAFVEFKANRTNVY
                     SMLFISLALATLSHIGYQASMAGATGSYFTRHLFPGAIVFQIACLFFLNVIISLVINR
                     QILSVAVIASLNVILVTANFLKFRYRSEPLTPNDFKWVGNLGMILSFISLRVVLVSLV
                     FIVLLVFIYRRIHKKYFQGRIVASIWKRLAGISVIVSLILGMGWAIRNEKDHKIAGWI
                     PILSQVNNWRNVDWKGYAFVARYRTLSFLWLQQLSKTSMEMPENYSEKTMKAIVKKYT
                     ALAEEINAERTGQLTDQTVIYILSESLADPRRIPGVTLSQNVLPNIEYIMSQTTSGLM
                     KSDHYGGGTANIEFQVYSGLPFYNYSSSISSVYLDVAPNMKKLPSISDLYPADNRIAI
                     HPYYDTSYNRNSIYKQLGIEQFYTLNSAKYPLTVTAEDYQGNFVSDKKTYDLILDQVR
                     SADTSFVSAITMQNHVQWNSLEPAGIIASGEGFTAEENENLTSYVRLLSFTDQATRDF
                     LDQLKTLDKKVTVVFYGDHLPGLYPESAFVTDPSAQYKTDYFVWSNFDTAKYNYELVN
                     SSDMNALMLETTNNKVSPYYALLTEVLHKDRVGQAERDAKVAEELKLVQYDLSTGKGY
                     LLKYKDFFKVATETTE"
     gene            complement(3922..5652)
                     /locus_tag="AC1PIW_000859"
     CDS             complement(3922..5652)
                     /locus_tag="AC1PIW_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605815.1"
                     /translation="MLKKLLKKHPDIVAVTVFFLFSLTLLLPQILTKAVVTGDDIVFH
                     YNRFYDTAIQIKSGDFSYIMSLHGYQQSGRIVNALYGPYLAYLQGALLLLAGTWYRYQ
                     LLSNLLLGVLSATSLYFLMREVKVLYSLSVGLSMFFATTYSVQYWWVAQGFSSWGVAL
                     FPLCLIPAVRFLKTGKVPIFLMAGAVGLMLQIHMLSALFLILAYAILFSIGWLKSQDK
                     WNVMKDVLFSVGVFLILTVNIWLPLLYVNATNELAAPFINKKFIQSTVTWSKAYFLYF
                     PYSLPIIFATSLYFMLKKWKKQSVILRGLTVTFLVFFIFSTNLFPWQLFAGKGIGLVD
                     LIQFPFRFFLYANALLLTIVGLQLTDYEVRKKLFLGLTILSTIIGVGFTWDQYNQAVN
                     DQYNKAEFLQDMLHTTLYGTTEELRASLHSKNLGEFVQLAQKSTPDYVPVLSEKEENV
                     RAVNEAKSRYDRYRDQIILPNQQFNKQVFGDSLKISWSANQSEEIQIPIVIYRDSELT
                     LNGKRLGKMDYYLSTIGVPTVTSQKGKNTLILSYKQQWWLLPVIFISLTGWILWSIYY
                     TFIYRKSNIS"
     gene            complement(6017..6889)
                     /gene="rsmA"
                     /locus_tag="AC1PIW_000860"
     CDS             complement(6017..6889)
                     /gene="rsmA"
                     /locus_tag="AC1PIW_000860"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIP6605816.1"
                     /translation="MRIADKNVTRAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DRNVNVIEIGPGIGALTEFLAENAAEVLAFEIDDRLIPILADTLGRFDNVTVVNQDIL
                     KTDLQTQIQNFKNPDLPIKVVANLPYYITTSILMHLIESKIPFSEFVVMMQREVADRI
                     SAEPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRREQPLVSVKD
                     EDFFFRVSKASFVHRRKTLWNNLTNHFGKSEEVKDKLTQALGMAELEASVRGEALSMA
                     DFARLSDSLQEVGL"
     gene            complement(6909..7439)
                     /locus_tag="AC1PIW_000861"
     CDS             complement(6909..7439)
                     /locus_tag="AC1PIW_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HIP6605817.1"
                     /translation="MKKVVLLRGVTPVGKNKIPKMSYLAEILTEAGLTSVQTYIQSGN
                     VVCETDILDEELSRLIHQTIKEKIGAELAIIVKHQMELTQAVAENSFGEGYDPSRVHL
                     VFTNDEINGEKLNKLLHQDFGDEELRLGSQCLYMYLPRDARKKKLNTNYLEKQLDITA
                     TMRKLSVISRLSEMAK"
     gene            complement(7453..7710)
                     /locus_tag="AC1PIW_000862"
     CDS             complement(7453..7710)
                     /locus_tag="AC1PIW_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HIP6605818.1"
                     /translation="MGIHFTDDAWEDYLYWQGQDKKTLKRINKIIQDMQRHPFEGIAK
                     PEPLKYDYQGAWSRRIDAENRLIYMVEADRLCILSLKDHYR"
     gene            complement(7712..7972)
                     /locus_tag="AC1PIW_000863"
     CDS             complement(7712..7972)
                     /locus_tag="AC1PIW_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIP6605819.1"
                     /translation="MEAIVYSHFRNHLKDYMKKVNDEFEPLVVVNKNPEEDIVVLSKS
                     EWDSLQETLAVARNTYLSQKVLRGMAKVKTGQTQERNLIEAD"
     gene            complement(8048..8503)
                     /locus_tag="AC1PIW_000864"
     CDS             complement(8048..8503)
                     /locus_tag="AC1PIW_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HIP6605820.1"
                     /translation="MSRTEQVILTNMCMITDGQQVLVQDRNSKKWPGVTFPGGHVEPN
                     ESIISSVIREIKEETGLTVSNLELCGIQNWTDPTDHYRYLVFCYKTSYFSGFIQSSDE
                     GEVFWIDRAELKNLQLADGFETMLEIFEQPQLTENFYWFEDGEWKVENR"
     gene            complement(8510..8839)
                     /locus_tag="AC1PIW_000865"
     CDS             complement(8510..8839)
                     /locus_tag="AC1PIW_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIP6605821.1"
                     /translation="MEFESYSVILAPAVEKELAVIYAYFSEQFSEEIAKRRIGMIVEA
                     LESLQIFPERGFNADNRFGKQIDPPHLTRGYVVGKDYIALYRVVKNEVRVGHLFATKS
                     DYVKLLK"
     gene            complement(8829..9116)
                     /locus_tag="AC1PIW_000866"
     CDS             complement(8829..9116)
                     /locus_tag="AC1PIW_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin subunit"
                     /protein_id="HIP6605822.1"
                     /translation="MITINKDAQVNFKTDSRLLEKAKEVFAMNQLDLTAGFNLFLQNI
                     AVKNELPILTEEELEREELFLQLQKEIQENQQAIEAGHGISLESVKEKYGI"
     gene            complement(9214..9582)
                     /locus_tag="AC1PIW_000867"
     CDS             complement(9214..9582)
                     /locus_tag="AC1PIW_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605823.1"
                     /translation="MDRVYAVKCLVKNEFEPSYENYSSYEEVTYLLPADVLVWEDEQV
                     DKNKLADYLLGLVEDGYVNADGIWKQSRLFHIAGVQEMDDRVEFDGLAVEVYRWFYNF
                     ERPVTEEEFLNLYYFDDLGR"
     gene            complement(9575..10144)
                     /gene="rnmV"
                     /locus_tag="AC1PIW_000868"
     CDS             complement(9575..10144)
                     /gene="rnmV"
                     /locus_tag="AC1PIW_000868"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HIP6605824.1"
                     /translation="MTQKIKIQEVLVVEGKDDTANLKRFYEVDTYETRGSAISDDDLE
                     RIEKLHDLRGVIIFTDPDYNGERIRKIIIQAIPTVKHAFLQRDEAAPKSKTKGRSLGI
                     EHASFEDLEKALAGVRGHYDDENNFDISQKTLIRLGLLMGGDSRKRREYLGEKLRIGY
                     CNGKQLLKRLELFGISLAEVEEAMESYHG"
     gene            complement(10128..10910)
                     /locus_tag="AC1PIW_000869"
     CDS             complement(10128..10910)
                     /locus_tag="AC1PIW_000869"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIP6605825.1"
                     /translation="MIQIFDTHTHLNVEQFDGRVQEELDFAKEMGVTLHNVVGFDRAT
                     IDKAMELADQHPEIYLTLGWHPTEAGTYDEEVENYLLKALQHPKVIALGEIGLDYYWM
                     TADKEIQARVFKRQMELAKQLDLPFVVHTRDALEDTYQIIKEVGVGPRGGIMHSFSGT
                     LEEAQAFMDLGMHISFSGVVTFKKAVELQEAAQALPLEKILVETDAPYLAPVPKRGRE
                     NRTGYTRYVVDMLAQLRNQSVEEVAAATYENARSLFRIDSEN"
     gene            complement(11017..11154)
                     /gene="rpmH"
                     /locus_tag="AC1PIW_000870"
     CDS             complement(11017..11154)
                     /gene="rpmH"
                     /locus_tag="AC1PIW_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIP6605826.1"
                     /translation="MKRTFQPSKIRRARKHGFRSRMATKNGRRVLAARRRKGRKVLTV
                     A"
     gene            complement(11322..12317)
                     /gene="jag"
                     /locus_tag="AC1PIW_000871"
     CDS             complement(11322..12317)
                     /gene="jag"
                     /locus_tag="AC1PIW_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HIP6605827.1"
                     /translation="MKFTGASVEEAIQNGLVELNIPRKKAHITVVAREKKGFLGFGKK
                     PAIVDIDVINETTVVKANQKAVRGVPSEINDLNEPVKSVSEATIDLGKVVAAVKEFEK
                     SGQTLDDDIKAQILKNEKEATTILEETGRIEILSEDLLTTTDATTSAKEDVNQEVISE
                     NQADNFADLDIKIEPQYDIEQVVSEVTAYVQEILDDMDVEARIDTSHNRRTINIQVDT
                     NEPGRVIGYHGKVLKALQLLAQNFLYNRYERNFYITINVNDYVEHRAEVLQGYAQKLA
                     ERVLAEQEAYHTDPMSSSERKIIHRIISKMDGLTSYSEGSEPNRYVVVDIEGNHD"
     gene            complement(12337..13149)
                     /locus_tag="AC1PIW_000872"
     CDS             complement(12337..13149)
                     /locus_tag="AC1PIW_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HIP6605828.1"
                     /translation="MKKKVKWAGLLVASLIVLTACGTGPVTSQSTDAWDRLIYWFASI
                     IQFLSINGQIGIGIILFTILIRTLLLPLFQIQMNSSRKMQELQPQLKKLQAEYPGTDM
                     ESRQQLYEATQALYKENGVSMRASMIPLFIQMPILLALFQALTRVEALKVGHFLWLNL
                     GEPDPFFILPILAAVFTFLSSWLTNKSAIEKNMAMTIMTYVMPVMIFFFAVAAASGVA
                     LYWTVSNAYQVAQTLVFNNPFKIIAERQAKIDAEKERQAKIRRAQKKAQKKK"
     gene            complement(13133..13492)
                     /gene="rnpA"
                     /locus_tag="AC1PIW_000873"
     CDS             complement(13133..13492)
                     /gene="rnpA"
                     /locus_tag="AC1PIW_000873"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIP6605829.1"
                     /translation="MKKSYRVKRERDFNDIFTKGNNVANRRFVVYRLPKEQKHFRVGL
                     SVSKKLGNAVMRNKIKRRIRHILIECQHQLVADDFVIIARKGVEELSYQEMKQNLIHV
                     LKLAKLYQEGVVIEKES"
     gene            complement(13563..14957)
                     /gene="argH"
                     /locus_tag="AC1PIW_000874"
     CDS             complement(13563..14957)
                     /gene="argH"
                     /locus_tag="AC1PIW_000874"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIP6605830.1"
                     /translation="MEAKKLWGGRFEASLEEWVEEFGASIRFDQKLAKYDIQGSLAHV
                     KMLGQTRIISQAEAQVIQAGLEELLEEYEAGKLVFDIRNEDIHMNIESLLTEKIGSVA
                     GKLHTARSRNDQVATDMHLYLKDTLFQVVDKLTNLRQILVDLAQEHVETIMPGYTHLQ
                     HAQPISFAQHLLAYYNMFSRDSERFAFNMQHTNVSPLGAAALAGTTFPIDRQMTSDLM
                     GFAKPYSNSLDAVSDRDFILEFLSNASILMMHMSRLCEEIINWCSYEYQFVTLSDTFS
                     TGSSIMPQKKNPDMAELIRGKTGRVYGNLVGLLTVMKSLPLTYNKDLQEDKEGMFDTA
                     ETVLISIDILAGMLKTMTVHKERMAQSTEKDFSNATELADYLASKGLPFRQAHEIVGK
                     LVLECSKAGHYLQDVSFETYQAISPLIQADIYDALSSKVAVSRRNSLGGTGFESIADQ
                     LKSAKEEIQNAKSL"
     gene            complement(14971..>16163)
                     /locus_tag="AC1PIW_000875"
     CDS             complement(14971..>16163)
                     /locus_tag="AC1PIW_000875"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HIP6605831.1"
                     /translation="TKEKLILAYSGGLDTSVAIAWLKKDYDVIAVCMDVGEGKNLEFI
                     HDKALSIGAIESHVLDVKEEFAQEYVLPALQAHAYYEQKYPLVSALSRPLISKKLVEM
                     AHKTGATTIAHGCTGKGNDQVRFEVAIAALDPSLKVVAPVREWKWAREEEIIFAKENG
                     VPVPADLDSPYSVDQNLWGRANECGVLENPWNEAPEDTFGITTSPENAPDTPEYVDIE
                     FKAGIPVAVNGEKLSLANLIQRLNVIAGKHGVGRIDHVENRLVGIKSREIYECPGAIT
                     LLTAHKEIEDLTLVREVSHFKPIVSNELSNLIYNGLWFNPATDALKAYLAQTQAVVNG
                     TAKVKLYKGSAKVVARKSPNSLYDEDLATYTSADTFDQDAAVGFIKLWGLPTKVNAEI
                     HKKS"
BASE COUNT         4696 a         3682 c         3038 g         4747 t
ORIGIN      
        1 ttcaattaca gacatattct tctccttttc ttgtccaata aataaattta cgcattatta
       61 ataataaatt ataaatatag gacaacaaaa tatgtccctt atacgcaacc actactcaat
      121 aagtatcttt tccaatatac tttcatacca atctgctata ttctggctat cgaccgctcg
      181 taaaagtcca atacactttc tcatctccaa aatagcatta gaagttccgg tattttgata
      241 atcataaaaa gccttaccaa atttaaacaa aatgcgttca aaaatttctg tctcgtcgaa
      301 aagcaaacct tcgatagtct tttcaagaaa gattgcgtcg acaagttcct tagtttgaac
      361 gctgactaga taagcactaa ggagaacttg agaaactacc aatcgatgtt gtttcatttc
      421 acggtaaaac tgtgtacgat gaattaactc tctacaaaaa agcatcattg tttgatgacc
      481 caatttgtca atcgtattcg caaatagtaa gatctcatag tagctccaat tctcaactcg
      541 aaataagtaa tccgttaaaa aggctgtatc ttctttcgaa acaactattg tcttatctaa
      601 gtttctcagt ttataagcga ttaaaatagc attcaaccga tgaaactccc tacgagaatc
      661 tctttctgct tttgctaatt gttcagacaa gattttcttt atggttacaa catctctgat
      721 atagatggat tgttttagtt gttccaaaat tctagtcagc tcatcttttt tgaaatcctg
      781 actggcatac acgaactcgt ccacatccac attcatttct gccagcacac caaataattt
      841 actaacagta atgtcggatt ccccattttc aaaccgagaa agttgagaag tagaaatatg
      901 tcccttggcg acctccgcca aactcttccc ctttccctct ctgataaatt tcaacgtttt
      961 cccaaaacaa ctcatttcca gacctccttt tgtatataag ggatttgttt aaagcataac
     1021 ttaatagatg ttatcatgat tttaaaataa atattatcta tattcaacaa tggggaatat
     1081 caatgaaaca aaattatcta attgcaaaca ttactatcgt tctaatttta cttattagta
     1141 tcctaaaaga cattccacca atcattgtta taaaataata atactcaacg aaaatcaaaa
     1201 gtagaccacc ttcaactatc ttccagatag ttgaaggtgg aaaatacaaa acaatgtttg
     1261 cgtcttcaag ccgacgatag aacactgtta ctttcttatt ttaaggtaac aggctgaaaa
     1321 gttttccatt catcaagact cttgacaacg gataattttg atttttgaag tgtataaaca
     1381 cctttattat accactcctt cccatatttg tgtccatttt cacataaaga aaaaccaagc
     1441 cagctatctg acttggtcgt gattttattc tgtcgtttca gtcgctactt tgaaaaaatc
     1501 tttgtatttc aaaaggtatc cttttcctgt agataggtca tattggacca gtttgagttc
     1561 ttctgcaact ttcgcatctc gctcagcctg tcctactcgg tccttatgaa gtacttctgt
     1621 caagagggca tagtaaggac tgaccttgtt attggtcgtc tccagcatca gtgcattcat
     1681 gtcgctagag ttaactagct cataattgta tttcgctgta tcaaaattgc tccaaacgaa
     1741 ataatctgtc ttatactgcg cacttgggtc cgtcacgaaa gctgactctg gatacaaacc
     1801 tggcaaatgg tccccgtaaa aaacaactgt cactttctta tccaaggtct taagctggtc
     1861 tagaaaatcc cttgttgcct gatccgtaaa ggataggagg cgaacatagc tggtcaagtt
     1921 ttcattttcc tcagccgtga agccctcacc cgatgctatt atacctgcag gctctagaga
     1981 attccactga acatggttct gcatggtaat agccgacaca aagctggtat cggcagaacg
     2041 tacctgatcc aaaatcaagt cataggtctt cttgtcactg acaaaattcc cctgataatc
     2101 ctcagccgtc acagtaagag gatacttggc actattgaga gtgtagaatt gctcaatccc
     2161 cagctgctta tagatagaat ttcggttgta actggtatcg tagtaaggat ggatggcaat
     2221 acgattatct gctggataga ggtcactgat acttggcaac ttcttcatat ttggcgccac
     2281 atccagatag actgaagaaa tggatgagct gtaattgtag aagggcagac cactatagac
     2341 ctgaaattcg atattagccg taccaccacc atagtggtca ctcttcatca agccactggt
     2401 agtctggctc atgatgtact caatatttgg aaggacattt tgactaaggg ttacacctgg
     2461 aatccgacgc gggtcagcca aactctcact aagaatgtag ataaccgtct ggtcagtcaa
     2521 ttgacctgtc cgttctgcat tgatttcctc agccaaggcg gtatacttct tcacaatggc
     2581 cttcatggtc ttctcagagt aattctcagg catttccata ctggtcttgc tgagttgctg
     2641 caaccagagg aatgaaagag tgcgataacg agccacaaag gcgtagccct tccaatctac
     2701 attccgccag ttattaacct gagaaagaat aggaatccag ccagcaatct tatggtcttt
     2761 ttcattgcga attgcccagc ccattcctaa aatcaaggat acgatgactg aaattcctgc
     2821 cagccttttc cagatagaag cgacaatcct tccttggaag tacttcttgt gaatacgtcg
     2881 gtagataaac acaaggagga ctataaagac gagcgatacc aagacaacac gtagactgat
     2941 aaagctgaga atcatcccca agttcccgac ccatttgaaa tcattggggg tcaatggctc
     3001 cgaacggtag cggaatttga gaaaattggc cgtaaccaaa atgacgttta gactagcaat
     3061 cacggcaaca gataagattt ggcggttgat aactaggcta atgatgacat tgaggaaaaa
     3121 gaggcaggca atctggaaga caatagcccc tggaaagaga tgacgtgtga aataagaacc
     3181 agtcgctcca gccatagaag cctgataacc aatatgcgat agcgtagcca gtgccaaact
     3241 gataaagagc atgctgtaga cattggttcg attggcctta aactctacaa atgccttatg
     3301 aagatagcga agaaaaatgt aggtccctat aaaatggata ggtgcaaaca agggtgtctg
     3361 gctgaaaaac tctatgtaag aacctttctc aaccaaactc tcataatggc tatctttcca
     3421 ataagatata gctagtggac tgagagaaag taagctggtc aagagcaaat agccatctgg
     3481 taccaagtga ttggctgacc actggcttaa tttttctcga attcccgtct tcttccgcca
     3541 taaaaatgcc aacaatacag gaagagccaa catcaataga agctcaaatt gaaagagatt
     3601 attccggaaa atattccagg gttgatactt cttggcattg agccgataga ccaggttgat
     3661 aagatataga aaacctagaa aatagccgac atacttggca aaggttttgg tctttataaa
     3721 tttctcatat ttatcaagca agaccaatac ataaaatgta aaaataagat aggcaatcaa
     3781 gggcaaggga ataaaccaga gtggaaaaga tacatcactc agtaaaaggg ctcgccccat
     3841 agccagcaag acggtcatta ccatgaccag aataaattgc ttggttgcct tgagtttgat
     3901 ttgtttcata gcatttctct cttaggaaat atttgatttt cggtagataa aggtataata
     3961 aatcgaccaa agtatccagc cagtcaaact gataaagata actggcaata accaccattg
     4021 ttgcttatag gagaggatta gggtgttctt ccctttttgg ctggtaaccg ttggcacacc
     4081 aatagtggaa agataataat ccatcttccc taacctcttt ccattcaatg tcaattcact
     4141 atctcggtaa ataacaatcg gtatttggat ttcctctgat tgatttgccg accaacttat
     4201 ttttaagcta tccccaaaaa cttgcttatt aaattgctgg ttaggtaaaa taatctggtc
     4261 acggtaacga tcataacgag atttagcctc atttaccgca cgaacattct cttctttttc
     4321 agataacacc ggcacatagt cgggagttga tttctgagcc agttgaacaa actcacctaa
     4381 attttttgaa tgcaaggatg cacgtaattc ttccgttgtt ccgtatagag tagtatgaag
     4441 catatcttgt aagaattctg ctttgttgta ctggtcattg actgcttgat tatattgatc
     4501 ccaagtaaaa ccaacaccaa ttatggtaga taagattgtc aaccccaaaa acaacttttt
     4561 acgtacttcg taatctgtta attgaagacc aacaatcgtc agaagtaaag cattagcata
     4621 caaaaagaat ctaaagggaa attggattaa atccactaat ccaatacctt taccagcaaa
     4681 tagttgccac ggaaaaagat ttgttgaaaa aataaaaaat accagaaaag ttactgtcag
     4741 acccctcaat atcaccgatt gcttcttcca ttttttcagc ataaaataaa gagaggttgc
     4801 aaaaatgatt ggtagagagt atggaaagta taagaaataa gccttagacc aggtaactgt
     4861 tgactgaata aactttttgt ttataaatgg tgctgctaat tcatttgttg catttacata
     4921 taataagggt aaccagatat ttacagttaa aatcagaaaa actccaaccg aaaacagtac
     4981 atctttcatt acgttccatt tgtcttgcga ttttaaccaa ccaattgaaa aaagaattgc
     5041 ataggctaag atgagaaaca gagccgacag catgtgtatc tgcaacatta accctacagc
     5101 tcctgccatc agaaaaatag gaactttacc agtttttagg aatcgaactg ctggaatcaa
     5161 acaaagcgga aataaggcca ctccccaact tgaaaatcct tgagctaccc accagtattg
     5221 aacagagtag gtagtcgcga aaaacatgga taacccaaca gatagagaat agagtacttt
     5281 cacttctcgc attagaaaat aaagcgaagt ggcactcaaa actcctaaaa gaagattaga
     5341 cagtagttgg tatcgatacc aagttccggc caacaacagc aaggctcctt gcaaataggc
     5401 tagatatgga ccatatagag cattgacaat ccttccagat tgctgatagc catgcaagct
     5461 cattatataa gaaaaatctc ctgacttaat ttgtatagct gtatcataaa aacgattata
     5521 atgaaaaaca atatcgtctc ctgtcaccac tgcttttgtc aaaatctgtg gcaatagaag
     5581 ggtgagcgaa aaaagaaaaa atacagttac tgcaacaata tctggatgct ttttcagtaa
     5641 tttttttaac ataagatacc ttcataaaaa aagagggaac ccctctcttg tttttgtaag
     5701 aacctgtact cagaagtgaa gtgattatca ataagagata gtttttcgct aatcaaggca
     5761 gttttattga gagaatactg actgtacttt gaaaaaacta acgatgacta gcttaaaaac
     5821 tatccttaag tagaagtgct gaactgtgaa tacgggttct tactattctg ctcatttgca
     5881 gacaataatt ccttaaaatt tatcaactcc cagtataaca agctttcccc ccactgtcaa
     5941 cgaacaaggc aaacaggaaa acaattttcc attaaaataa atattgattg cagatttctc
     6001 cgcaatcaat acttgcttat aaaccaactt cctgaagact gtcagacaaa cgtgcgaagt
     6061 cagccatgga aagtgcttcg ccacgaacac tggcttccag ctctgccatg ccaagtgcct
     6121 gcgtcagctt gtccttgact tcttcggatt tgccgaaatg gttggtcagg ttgttccaca
     6181 gggtcttgcg gcgatgcaca aagctggcct tggacactcg gaagaaaaaa tcttcgtctt
     6241 tgacgctcac cagaggttgc tcgcggcgga ccattttgag aatggcggag tccacgtttg
     6301 gtgcaggcac aaagacggtg cgaggcacga taaaggccac cttggcagtc atgtagtact
     6361 ggaccgcaat ggacaggctt ccgtaagcct tggtgtttgg ctcagcagaa atgcggtcgg
     6421 ccacctcccg ctgcatcatg accacgaact cgctgaaggg aatcttgctc tcaatcaggt
     6481 gcatgaggat agaggtggtg atgtagtagg gcaggttggc cacgaccttg atgggcaaat
     6541 cagggttctt aaagttctga atctgggtct gcaggtcggt cttgaggatg tcttggttga
     6601 ccacggtcac attgtcaaag cgacccaagg tgtccgccaa aatcggaatc aggcggtcgt
     6661 caatctcaaa agccaagacc tcagcggcat tctccgccaa aaactcggtc aaggccccga
     6721 ttccaggacc aatctcgatg acattgacat ttcggtcaat ctctgccgtg tccacaatct
     6781 tctgtaaaat attggtatca gtcaaaaaat tttgaccgaa ggatttctta aaggtaaacc
     6841 cgtggcgttc aaggacggca cgagtaacgt ttttgtctgc aattctcatg tgtgttcttt
     6901 cttgtttttt atttcgccat ttcactcaat cgtgaaataa cacttagttt tctcatcgtc
     6961 gctgtaatgt ccagctgttt ttctaaataa ttggtattta gttttttctt tcgagcatct
     7021 ctcggaagat acatgtatag acattgactt cccagacgca actcctcatc gccaaagtct
     7081 tggtgcaata atttgtttaa tttttcacca tttatctcat cattggtaaa gaccaaatga
     7141 acacgagaag gatcatagcc ctcaccaaaa gaattttcag caactgcttg tgttaactcc
     7201 atctgatgtt tgacaatgat cgctaactct gcacctattt tttctttaat ggtttgatgg
     7261 ataagccgac tgagttcttc atctaatata tctgtttcac aaacaacatt tccgctttga
     7321 atataggttt ggacagaagt taaaccagct tccgtcaaaa tttccgccaa gtaagacatc
     7381 ttgggaattt tattcttgcc aacaggggta acacctcgaa ggaggacgac ttttttcatt
     7441 tgttaatccc cctcacctat aatgatcctt tagcgacagg atgcacaacc gatcagcttc
     7501 caccatataa atcaaccgat tctccgcatc aattctccga gaccaggcac cttgataatc
     7561 atacttcaaa ggttctggtt tggcaatgcc ttcaaaagga tgtcgctgca tatcctggat
     7621 aatcttattg attctcttca gtgttttctt atcctgaccc tgccaataaa gatagtcttc
     7681 ccaggcgtcg tctgtaaaat ggattcccat gttagtccgc ctctatcagg tttcgttctt
     7741 gggtttgtcc cgttttgact ttagccatac cacgtaggac tttttgagac agataagtgt
     7801 tccgagcaac agcgagggtt tcttgtaaac tatcccactc actctttgag agtacaacaa
     7861 tatcttcctc tggattttta ttaaccacga ccaaaggttc aaactcgtca ttgacctttt
     7921 tcatatagtc ctttaagtga tttcgaaaat gggaatatac aatagcttcc ataagcaata
     7981 cctctcatca tttctaggct tactatagca tagaaaaaaa gagttgtcaa acttattgta
     8041 caactcttta acgattctcc accttccatt cgccgtcttc aaaccagtaa aaattctccg
     8101 tcagctgcgg ctgttcaaag atttccaaca tggtttcaaa accgtcagca agttggagat
     8161 tcttcaattc tgcacggtca atccagaaga cctcgccctc atcagaagat tggataaaac
     8221 ctgaaaaata actggttttg tagcaaaata ccagatagcg ataatggtca gtaggatccg
     8281 tccagttttg aatgccacag agttcgagat tggataccgt caagcccgtt tcttccttga
     8341 tttcacgaat gacggaggaa ataattgact catttggctc gacatggccc ccagggaaag
     8401 tcacaccagg ccatttcttt gaattcctat cctgaactaa tacctgctga ccgtctgtaa
     8461 tcatgcacat attggttagg atgacttgct ctgtcctact catgctctct cacttcaaca
     8521 acttcacata atcactcttg gtcgcaaaaa gatgaccaac tcgaacttca tttttcacta
     8581 cgcgatagag ggcgatgtag tcttttccaa cgacatagcc tcgggttaga tgaggcgggt
     8641 ctatctgctt accaaaacgg ttatcggcgt taaaaccacg ttcaggaaaa atttgcaaac
     8701 tctctaatgc ttcgacaatc atcccaattc gtcttttggc aatttcttct gagaactgtt
     8761 ccgaaaaata ggcgtaaata actgccaatt ccttctccac tgctggagca agtatcactg
     8821 aataagactc aaattccata cttctctttg acactttcta ggctgatccc gtgacctgcc
     8881 tcaatcgcct gttggttttc ctggatttcc ttctgtaact gtaaaaacaa ctcttctctt
     8941 tccaactctt cttctgtcag aattggcaac tcatttttta cagcgatatt ttgcaagaag
     9001 aggttaaaac cagcagtcaa atccagctgg ttcatagcaa atacttcctt ggctttttct
     9061 aaaagtctgg aatcagtttt aaaatttact tgggcatctt tatttatagt tatcaatcta
     9121 gtccacctcc tagttttcta tattttaaca caaatagaca ccgctagcaa ttttcaaaaa
     9181 agcagaaata gtaatatttc cgctctagat tgcctatctc cccaaatcat caaaataata
     9241 gagattcaaa aattcttcct cagtaactgg acgttcaaaa ttgtaaaacc agcgatagac
     9301 ttccacggct agtccatcaa actctaccct gtcgtccatt tcttgcacac cggcaatatg
     9361 aaacaaacgg ctctgcttcc aaataccatc cgcattgaca tagccatctt caactaaacc
     9421 aagcaaatag tctgctagtt tgttcttatc aacctgctca tcctcccaaa caaggacatc
     9481 agccggcagc aagtaggtca cttcttcata agatgaataa ttttcataag aaggttcaaa
     9541 ctcgttttta accaagcact taacagcgta gaccctatcc atgatagctc tccatagcct
     9601 cctcaacctc cgccaaactt atcccaaaca attccaatcg tttgaggagt tgtttgccgt
     9661 tgcagtagcc gatgcggagc ttctctccca gatactccct ccgcttgcga ctgtcgccac
     9721 ccataagcaa ccctagccga atcagggtct tctggctaat atcaaagtta ttttcatcat
     9781 cgtaatgacc acgtacgcct gctagggcct tttccaagtc ttcaaagctg gcgtgctcaa
     9841 tgcccaagga ccgccctttt gttttggact tgggggcagc ctcgtcccgc tgtaaaaaag
     9901 cgtgtttgac cgtgggaatg gcctgtataa tgatttttcg aatccgctcg ccattgtagt
     9961 ctgggtcagt aaagataatc acgccacgca ggtcatggag tttctcgatg cgttccaagt
    10021 catcgtctga tatggcagag cctctggttt cgtaggtatc cacctcgtaa aaccgcttca
    10081 agtttgccgt gtcgtccttg ccttcaacaa cgaggacttc ttgtatctta attttctgag
    10141 tcaattctga acaaactcct tgcattctcg taggtcgcag ccgccacttc ttcgaccgac
    10201 tggttgcgaa gctgggccag catatccacc acatatctgg tgtagccggt gcgattttcc
    10261 cgaccacgct tgggaacagg cgccaggtag ggtgcgtccg tttcgaccag gattttttct
    10321 aagggcaatg cttgcgccgc ttcctgaagt tcaacggctt ttttgaaggt caccactccc
    10381 gagaaggaga tgtgcatgcc caggtccatg aaagcttgag cctcctccag agtgccagag
    10441 aaggaatgca tgatgcctcc acgcggaccg acgccgactt ccttgatgat ctggtaagta
    10501 tcttccaaag catcgcgagt atgaaccaca aagggcaaat ccaactgctt ggccagctcc
    10561 atctgtcgct tgaaaacacg agcctggatt tccttatccg ccgtcatcca gtagtaatcc
    10621 agtccaatct ctcccagggc aatgaccttg ggatgctgga gggctttgag cagatagttt
    10681 tccacttcct cgtcataagt gccagcctct gtgggatgcc agcccagtgt cagataaatt
    10741 tcagggtgct ggtcagcaag ctccatagcc ttgtcaatgg ttgcacggtc aaaaccgacc
    10801 acattgtgga gggtgacccc catctccttg gcaaagtcta attcttcttg gacccgaccg
    10861 tcaaactgct ctacgttgag gtgggtatgg gtatcaaaaa tttgtatcat cgttccatca
    10921 ctttctttcc tactattgta acatatttca gaagattctt atgagaagaa aaaaaccagc
    10981 agactcgagt ttctgctggc ttctcttttc atcttcttat gctactgtaa gaactttacg
    11041 tcccttacgg cgacgagcag ctaacacgcg acggccgttt ttagttgcca tacggctacg
    11101 gaaaccgtgt ttacgcgcgc gacggatttt acttggttga aaagtacgtt tcacgatgaa
    11161 tacctcctct tagattcttg tattcgttta gccggctagt tttgatcagt tactaaacat
    11221 accaaactat tttatataaa ttctgttcgt ttgtcaagct attctgccat acttttagga
    11281 aagaatcaga aaatcattga aaaaagagga ctagcctctt tttaatcatg atttccttca
    11341 atatctacaa cgacatatcg gttcggctcg ctaccttctg aataacttgt caaaccatcc
    11401 attttagaga taatgcggtg gataatttta cgttcgctac ttgacatcgg atctgtatgg
    11461 taggcttcct gctctgccaa aacacgttct gccaattttt gtgcgtagcc ttgaagaacc
    11521 tctgcgcggt gttctacata atcattcaca ttgattgtga tatagaaatt acgctcataa
    11581 cggttgtata agaagttctg agccaaaagt tgcaaggctt tcaaaacttt tccgtgataa
    11641 ccgataacac gacctggttc attggtatcc acttgaatat tgattgtacg gcgattatga
    11701 cttgtatcaa tacgcgcctc aacatccata tcatccagaa tttcttgtac ataagctgtc
    11761 acttccgaaa caacttgttc aatatcatac tggggctcaa tcttaatatc taaatcagca
    11821 aaattatctg cttgattttc tgagatgact tcttgattta cgtcttcctt agcagatgtt
    11881 gttgcatctg tagtagttag taaatcctct gaaagaattt caatccgacc tgtttcttcc
    11941 aaaatcgttg tcgcttcttt ttcatttttc aagatctgcg ctttaatatc atcatcaagc
    12001 gtttgaccag acttttcaaa ttctttcacc gctgcaacaa cttttcctag gtcaatcgta
    12061 gcctctgaaa cgctttttac tggttcattg aggtcattaa tttcacttgg aacgccacga
    12121 actgctttct gattagcctt aacaaccgtt gtttcattga tgacatcaat atccacaatc
    12181 gctggctttt taccgaaacc aaggaaacct tttttctcac gcgcaaccac agtaatatgt
    12241 gctttttttc gaggaatatt cagctctacc aagccattct ggatggcctc ttcaacactt
    12301 gctcctgtaa atttcatctt ctctctccta gttttcctat ttctttttct gtgctttttt
    12361 ctgtgcccgt cggatttttg cctgacgctc tttttcagca tcaattttcg cttgacgctc
    12421 tgctattatt ttaaacggat tattgaacac caaggtttga gcaacctgat aggcatttga
    12481 cactgtccaa tacaaagcca ccccactagc agctgccaca gcaaagaaaa agatcatcac
    12541 gggcatgaca taggtcataa ttgtcatagc catattcttc tcaattgctg atttattggt
    12601 caaccatgag ctgaggaatg taaacactgc agctagaatc ggcaaaataa agaacggatc
    12661 cggctcccca agatttaacc aaaggaagtg acctactttc aaggcttcaa cacgtgtcag
    12721 agcttgaaag agggccaaaa ggattggcat ttgaataaag agcggaatca tggacgctct
    12781 catgctgacg ccattctctt tgtagagagc ttgcgttgct tcataaagct gctggcgact
    12841 ttccatatcc gttcctggat actccgcttg caactttttc agctgaggtt gaagctcctg
    12901 catcttccgt gaggaattca tctgtatttg gaacaacggc aacagaaggg ttcgaattaa
    12961 aatggtaaaa agtataatac cgattccaat ttgaccgttg atagatagga actgaataat
    13021 gctggcaaac cagtaaatta atctatccca cgcatctgta gattgactgg tcactggccc
    13081 tgttccgcag gctgttaaaa caattagaga tgctaccagt agacctgccc atttaacttt
    13141 ctttttcaat cacaacacct tcctggtata gtttagctag tttcaaaaca tggatgaggt
    13201 tttgcttcat ttcttgatag gacaattcct ctacaccttt tcgagcaatg ataacaaagt
    13261 catctgctac cagttgatgt tggcattcaa ttaagatgtg acgaatcctg cgcttaatct
    13321 tatttcgcat gacagcgttt cccaattttt tgctgacaga taaacctact cggaaatgct
    13381 tttgctcttt aggtaatcgg tagacaacaa acctccgatt ggcgacattg tttccttttg
    13441 taaaaatgtc attaaaatct cgttcacgct tgacacgata gctttttttc aaactgccac
    13501 tccatctttc aaaattactt ctattatacc acaaaattca aaaaagccaa taaacatacc
    13561 gtttataggc tttttgcgtt ctgaatttcc tctttggcac ttttcaactg gtctgcaatc
    13621 gattcaaaac ctgtcccgcc aagggaattt ctacgactaa cagcaacttt ggaggacaag
    13681 gcatcataaa tatctgcttg aataagaggc gaaatagcct gataggtctc aaatgataca
    13741 tcctggagat agtgtcctgc cttgctacac tccaagacca gttttccaac aatctcatgc
    13801 gcttggcgga agggcagacc tttactagcc aaataatcag ccaactctgt agcatttgaa
    13861 aagtccttct ctgtcgattg agccatcctt tccttatgaa ccgtcatcgt tttcagcata
    13921 cccgccaaaa tatcaattga aatcaaaact gtttctgccg tgtcaaacat gccttccttg
    13981 tcttcctgta agtctttatt ataggttaaa ggcagggatt tcatgactgt cagtaggcca
    14041 actaagttcc cataaactct acctgttttc ccccgaatca gctcagccat atctggattt
    14101 ttcttctgag gcatgatgga agaacctgtt gaaaaggtat cggatagggt gacaaattgg
    14161 tattcatatg aacaccagtt aatgatttct tcacacaaac gcgacatgtg catcatgagg
    14221 atactggcat ttgacagaaa ttccaagata aaatcacggt cagacacagc atcaagagaa
    14281 ttactatagg gcttggcaaa tcccatcaaa tcagacgtca tctgacggtc aatcggaaag
    14341 gtcgtccctg ccaaggccgc cgcaccaagt ggggatacat ttgtatgctg catgttgaat
    14401 gcaaagcgct cactgtctcg gctgaacatg ttgtaatagg ctaacaagtg ctgagcaaaa
    14461 ctaatgggct gggcatgttg caagtgggta tagccgggca tgattgtttc cacatgctcc
    14521 tgtgcaaggt ctactaaaat ctgacgtagg ttggtcaatt tgtctacaac ttgaaagagt
    14581 gtatccttca aataaaggtg catatccgta gccacttggt cgttacgaga acgagctgta
    14641 tggagtttgc ctgcaactga gcctattttc tccgtcaata gactttcaat attcatgtga
    14701 atgtcttcgt ttcgaatatc aaataccagc ttgccagcct catactcctc tagcaattct
    14761 tccagaccag cttgaatgac ctgagcctca gcttgagaga taatcctagt ttgccccagc
    14821 atcttgacat gggccagtga gccttgaata tcatacttgg ccaatttttg gtcaaagcga
    14881 atggaggctc cgaattcttc aacccattcc tcaagactgg cttcaaagcg acctccccat
    14941 aatttctttg cttccatagg ctcctacttt ctagctcttt ttatgaattt ccgcattgac
    15001 cttggttggc aatccccaaa gcttgatgaa accgactgcc gcatcttgat caaaggtatc
    15061 tgcactggta taggttgcca aatcttcgtc ataaagggaa tttggagatt tccgagcaac
    15121 tactttggct gaccccttat acaatttcac ttttgcggtg ccattaacaa ctgcctgggt
    15181 ctgagcaaga taggccttca aggcatctgt tgctggattg aaccacaaac cattgtaaat
    15241 caagttagag agttcatttg aaacaatcgg tttgaaatgc gatacttccc gtaccagggt
    15301 caaatcttca atttccttgt gggctgtcag aagggtaata gctcctgggc actcgtaaat
    15361 ttcccgtgac ttgattccga ctaaacgatt ttccacgtgg tcaatccgac ccacaccatg
    15421 tttaccagca atgacattga gtctttgaat gagattagca agacttaact tttcaccatt
    15481 tacagcaaca ggaatacctg ctttaaactc aatatcgaca tactcgggag tatctggtgc
    15541 attttctgga gaagtagtga tgccaaaagt atcttctggc gcttcattcc atggattttc
    15601 caagacacca cactcattgg cacgacccca gaggttttgg tcgactgagt aaggactgtc
    15661 cagatcggca ggaactggaa caccattttc cttggcgaag atgatttctt cttcacgcgc
    15721 ccacttccat tcacggacag gagccacgac tttcaagctt ggatccaggg ctgcaatggc
    15781 tacctcaaac cgcacctggt catttccctt accagtacag ccgtgggcga tagtggttgc
    15841 tcctgtctta tgagccattt ccaccaactt tttggaaatc aagggacggc tgagagccga
    15901 taccagcgga tatttttgct catagtaggc atgggcttgt agggcaggca aaacgtattc
    15961 ttgagcaaat tcttctttca catccaaaac atgcgattca attgcaccga tggaaagggc
    16021 cttgtcatga ataaattcga gatttttccc ttcaccaaca tccatacaaa ccgcaatgac
    16081 atcatagtct ttcttcaacc aagcaatcgc aactgaagta tcaagtccac cagaataggc
    16141 caaaattaat ttttcttttg tca
//
LOCUS       DBNFCK010000034        15704 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.034, whole genome shotgun sequence.
ACCESSION   DBNFCK010000034 DBNFCK010000000
VERSION     DBNFCK010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 15704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15704
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.034"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            74..526
                     /locus_tag="AC1PIW_000876"
     CDS             74..526
                     /locus_tag="AC1PIW_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605832.1"
                     /translation="MIKFLRKKPTIEQLKKVPYASQYTEVLRSIWRADVPKYGISSTL
                     QGELLRQLEKLRWEAQANGNVNWCEEHSNYCRFIKETLYKGKLLSSQQKQELVLIMDY
                     LKSCGEYAQAYQENLIDDEELEIEKLAYVDDNLYDRVGDMIAFFYQRT"
     gene            727..3180
                     /gene="parC"
                     /locus_tag="AC1PIW_000877"
     CDS             727..3180
                     /gene="parC"
                     /locus_tag="AC1PIW_000877"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HIP6605833.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQERALPDIRDGLKPVQRRI
                     LYSMNKDGNTFDKGYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQDWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEMAGYLLADIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGATGISAGYATDIPPHNLAEVIDAVVYMIDHPTAKLEKLMEFLPGPDFPTGAI
                     IQGADEIKKAYETGKGRVVVRSRCEIEQLKAGKKQIVITEIPYEVNKAVLVKKIDDVR
                     VNNKVPGIAEVRDESDRTGLRIAIELKKDSDEQTILNYLYKYTDLQINYNFNMVAIDN
                     FTPRQVGLQKILSSYIAHRREIIIARSKFDKEKAEKRLHIVEGLIRVVSILDEVIALI
                     RASENKADAKENLKISYEFSEEQAEAIVTLQLYRLTNTDIVTLENEEAALREQIQTLA
                     AIIGDERTMFNLMKKELREVKKQFGNPRLSELQDQAETIEIDTASLIVEEETFVSVTK
                     AGYIKRTSPRSFNASTLEEMGKRDDDQLIFLQNAKTTQHLLLFTNLGNVIYRPVHELT
                     DIRWKDIGEHLSQTLMNFDTNEEIIFAELVENFEEGTYFAVTKYGQIKRVERKEFTPW
                     RTYKSKSTKYAKLKDAEDVVITVSPVVLDDIMLMTEKGYALRFNIEEVPIIGAKAAGV
                     KAVNLKDEDVVAAAFISNTNSVYLLTQRGSLKRMATEEIPVTSRAKRGLQVLRELKAK
                     PHRVFAAGPVLTDQGDFDLFSTANEDDTTSQILHVQSKTGKLYEVDVTQLSLSERTSN
                     GSFISDTISDEEVFRAWID"
     gene            3243..3860
                     /locus_tag="AC1PIW_000878"
     CDS             3243..3860
                     /locus_tag="AC1PIW_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HIP6605834.1"
                     /translation="MKIIKLFFSIKVYLWLVLSFLLLLCGVWGYGYISGEKSNQQEVS
                     VHAAIESVEQVNELVFLNTAVQKIVTAKNYTVLFGQEMPFSEKSALLIIKYKAKFGIK
                     SPVSIEHLSENRYKITLPAFEVIGLELDAEEPYTLYDKSGEILSFATEEIDTAQLITD
                     EFQSVEQEKFLADYQEDIKESAKNYYQNLFSAISPEIQLEFVFKE"
     gene            3971..4993
                     /locus_tag="AC1PIW_000879"
     CDS             3971..4993
                     /locus_tag="AC1PIW_000879"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HIP6605835.1"
                     /translation="MTVNIDWENLGFSYMKLPYRFIAHFKNSQWSEGQLTEDAELHIS
                     ESSPALHYGQQAFEGLKAYRTKDGKLQLFRPDQNAERLQRTADRLLMEPVPTDLFIKA
                     CKEVVKANADYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTVFAMPVGNYFKGG
                     LAPTNFLIQDKYDRAAPNGTGAAKVGGNYAASLLPGQYAKKQGFSDVIYLDPATHTKI
                     EEVGSANFFGITADNEFVTPISPSILPSITKYSLLYLAEHRLGMKAVEREVLVEELDQ
                     FVEAGACGTAAVISPIGGIQIADKLHVFHSETEVGPVTRKLYEELTGIQFGDIEAPEG
                     WIVEVD"
     gene            5060..5293
                     /locus_tag="AC1PIW_000880"
     CDS             5060..5293
                     /locus_tag="AC1PIW_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HIP6605836.1"
                     /translation="MSKKDKKIEIQIEDSKVLVNREEFPGYRLVIGKKVIGEIAELAE
                     NNFAVIKNGNTESFYKKLEKAVGNIIENYNLSH"
     gene            5345..5425
                     /locus_tag="AC1PIW_000881"
     tRNA            5345..5425
                     /locus_tag="AC1PIW_000881"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5379..5381,aa:Tyr,seq:gta)
     gene            5701..5772
                     /locus_tag="AC1PIW_000882"
     tRNA            5701..5772
                     /locus_tag="AC1PIW_000882"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5733..5735,aa:Gln,seq:ttg)
     gene            5896..7101
                     /gene="rpsA"
                     /locus_tag="AC1PIW_000883"
     CDS             5896..7101
                     /gene="rpsA"
                     /locus_tag="AC1PIW_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HIP6605837.1"
                     /translation="MNEFEDLLNSVSEVTPGDVVTAEVLTVDAGQANVAISGTGVEGV
                     LTLRELTNDRDADINDLVKVGETLELLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKVTRAVKGGLAVEFEGLRGFIPASMIDSRFTRNTERFVGQEFDAKIKE
                     VDPAENRFILSRRDVVEAAAAAARAEVFGKLAVGEVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSAVTVGEEVEVKVLAIDEVEGRVSLSLKATQPGPWDGVEQKL
                     VAGDVIEGKVKRLTDFGAFVEVLPGIDGLVHISQISHKRVENPKDALSVGQEVTVKVL
                     EVNAADERVSLSIKALEERPAAVEGEEKAEKRAPRPRRQKREEKRDYELPETQSGFSM
                     ADLFGDIEL"
     gene            7139..7210
                     /locus_tag="AC1PIW_000884"
     tRNA            7139..7210
                     /locus_tag="AC1PIW_000884"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7172..7174,aa:Arg,seq:ccg)
     gene            7272..8300
                     /locus_tag="AC1PIW_000885"
     CDS             7272..8300
                     /locus_tag="AC1PIW_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004793 - threonine aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HIP6605838.1"
                     /translation="MLHFENDYNKGAHPALLEALVASNQEGLSGYGTDKYTESAIEKI
                     RLATDCPHAQVTFLAGGTQTNQIVISSMLASYEGVIAADTGHIAVHEAGAIEFTGHKV
                     LALPHTDGKLVASEVENYISDFYADANHAHMVHPGMVYISHPTEYGTLYSKSELEALS
                     TVCRHHNIPLFLDGARLGYGLAARDTDLDLKTIAELTDVFYIGGTKLGALMGEALVFT
                     KNNQPKNFVSIVKHHSALLAKGRVTGIQFDCLFTDDLYLELGRHAIAMAEQLTQILEE
                     KGFQFYLKSPTNQQFVIVKNEELTRLTEVGIAYGFWEKYDDSHSIIRFATSWSTSQAD
                     IDALRNRL"
     gene            complement(8319..8702)
                     /locus_tag="AC1PIW_000886"
     CDS             complement(8319..8702)
                     /locus_tag="AC1PIW_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605839.1"
                     /translation="MKYVILKTPIFVWLLIFALIQIFALSSFLPEPFDTRSLLPMGVL
                     TGCSIRVSSEVKDQDGQYAPFKYLWWVILGWTVIGILFGITIKAPLELHIALIVHAIA
                     GGIGYRLIERYAVYRYHYLQTKKKG"
     gene            complement(8763..9176)
                     /locus_tag="AC1PIW_000887"
     CDS             complement(8763..9176)
                     /locus_tag="AC1PIW_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6556 family protein"
                     /protein_id="HIP6605840.1"
                     /translation="MNTRKNTNKKPAGLTNFQKYLTFIGSILGIITACITIYTFTAKP
                     ATSSTSPSTTISSSSATSPSQSSSDNPLTEVQDSTSSSSETTSSTQTETSSLVESTVS
                     SSSTETSATQALETRTETSTDTEVTSGASETTSNQ"
     gene            9289..10431
                     /locus_tag="AC1PIW_000888"
     CDS             9289..10431
                     /locus_tag="AC1PIW_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIP6605841.1"
                     /translation="MIYFDNAATTQVYPEVLKTYTEVATKIWGNPSSLHSLGSQATRI
                     LEASRKQIAELLGQDSKEIFFTSGGTEGDNWVIKGVAFEKAHLGKHIIVSAIEHPAVK
                     ESALWLKTQGFEVDLAPVNAQGFVDVSALESLIRPDTTLVSIMAVNNEIGAIQPIQEI
                     SQLLADKPTISFHVDAVQAIGKVPTERYLTDRVDFASFSGHKFHSVRGVGFVYIKAGK
                     KIAPLLTGGGQESDKRSTTENVAGIAATAKALRLTLDKAADSQKRLAAMKQILVDELG
                     KYDDVTVFSGLEDFVPSIVTFGIKNIRGEVIVHAFEDHQIYISTTSACSSKAGKPAGT
                     LIAMGVPQKLAQTAVRISLDDDNDMGQIEQFLTIFKQIYQNTQKVR"
     gene            10433..11650
                     /gene="thiI"
                     /locus_tag="AC1PIW_000889"
     CDS             10433..11650
                     /gene="thiI"
                     /locus_tag="AC1PIW_000889"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HIP6605842.1"
                     /translation="MNYSEIMIRYGELSTKGKNKMRFVNKLRNNIKHVLSVYPEVTVY
                     FDRDRGHVYLNGADYQEVSASLKKIFGIQNFAPSYKIEKSVPALKEAVVEIMQTIYKE
                     GMTFKIAARRSDHSFELDSRDLNQVLGDAVFTAIPNVQVQMKSPDITLRVEIRPDAAY
                     ISHEEIKGAGGLPVGTSGKGTLMLSGGIDSPVAGYLALKRGVEIEALHFASPPYTSPG
                     ALKKAHDLTRKLTAFGGNITFIEVPFTEIQEEIKEKAPEAYLMTLTRRFMMRITDRVR
                     EERGAMVIINGESLGQVASQTLESMQAINAVTNTPVIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNVEQYEARMDVEGLVERAVAGIIVTEITPKEE
                     VKDEVDSLIEDLL"
     gene            11769..13097
                     /gene="brnQ"
                     /locus_tag="AC1PIW_000890"
     CDS             11769..13097
                     /gene="brnQ"
                     /locus_tag="AC1PIW_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HIP6605843.1"
                     /translation="MMKKGALTGLLLFGMFFGAGNLIFPPALGVLSGENFWPAILGFV
                     VSGVGIAVIALIVGTLNPKGYVHEISRKISPTFATIYLVALYLAIGPFFAIPRTATTS
                     FEIGIAPLLGDANLGLWLFGFTALYFVAAYLIALNPSQILNSIGRILTPVFAILIVIL
                     VVLGIVKYGSTSPLPASEAYSAGQAFGTGFIEGYNTLDALASIAFSVVAVNTLKQLGF
                     SSKREYVSTIWSVGLVVALAFSALYVGLAFLGNHFPVPADVLASDTNKGVYILSQATQ
                     AIFGPGAQIFLAAMVIVTCFTTTAGLIVSSGEFFAERFPRFSYKVYATIFTLIGFGIA
                     NLGLNNIITFSVPVLLVLYPITICIVLITIVNKFVPLSTYGMQLTVGLVTALSLVEVL
                     AGQFNWTAVSKIISALPLAGQSLAWLLPALVGIVLSLFLPNKQESEVFEM"
     misc_feature    13164..13252
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            13288..13602
                     /gene="rplU"
                     /locus_tag="AC1PIW_000891"
     CDS             13288..13602
                     /gene="rplU"
                     /locus_tag="AC1PIW_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIP6605844.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAIYVEKLNVEAGQEVTFEEVVLVG
                     GEKTVVGTPLVAGATVVGTVEKQGKQKKVVTFKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            13649..13942
                     /gene="rpmA"
                     /locus_tag="AC1PIW_000892"
     CDS             13649..13942
                     /gene="rpmA"
                     /locus_tag="AC1PIW_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIP6605845.1"
                     /translation="MLNLNLANLQFMAHKKGGGSTSNGRDSQAKRLGAKAADGQTVSG
                     GSILYRQRGTKIYPGANVGRGGDDTLYAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            14134..14292
                     /locus_tag="AC1PIW_000893"
     CDS             14134..14292
                     /locus_tag="AC1PIW_000893"
                     /inference="COORDINATES: protein motif:HMM:NF012792.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6605846.1"
                     /translation="MFRGSGMGSNLVKLVIDDLANRNSKAFQIAVEEEKLGTWKLYKK
                     LVLKNRTG"
     gene            14375..15289
                     /locus_tag="AC1PIW_000894"
     CDS             14375..15289
                     /locus_tag="AC1PIW_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIP6605847.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISIRDLEKELG
                     FQIFSRTSSGTFLTQKGMEFYEKAQALVKGFDQFEHLYLQPEEGEKTFSISSQHYDFL
                     PPLITEFSRSHPQYPHFRIFESTTVQILDEVAQGYSELGIIYLNERNTKGIMQKLDKL
                     HLQAVDLSAFQTHIYLREDHPLTKKAEIDPVDLVGLPTVRFTQEKEAYLYYSENLIDT
                     SDSSVVFDVTDRATLNGILERTDAYATGSGYLDDESVNGITVIPLKGKVDNRMVYVKR
                     ANDELSSCAKDFIETMQAYFDKKKDENA"
     gene            15282..>15704
                     /gene="lspA"
                     /locus_tag="AC1PIW_000895"
     CDS             15282..>15704
                     /gene="lspA"
                     /locus_tag="AC1PIW_000895"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIP6605848.1"
                     /translation="MRKIGFPFLMVVLIGLDQFVKAWTVANIELDTVTEFIPGLMSLA
                     YLRNYGAAWSILQNQQWFFTIMTIVAVTGLVWYYIKQIKGNIWTLFSLSLMIAGALGN
                     FIDRLRLGYVVDMFHLDFISFPVFNVADVCLTVGVGILF"
BASE COUNT         4578 a         2985 c         3606 g         4535 t
ORIGIN      
        1 tgaaattaat caaaacagat gtcgatgact ttagaagctg gcaagcaagc cagtggaagg
       61 cggaagagta gctatgataa agtttttaag aaaaaagcca actatcgagc aacttaagaa
      121 agttccttat gcttctcagt atacagaggt tctacgttca atttggcgag cagatgtgcc
      181 taaatacggg atttcttcta ctttacaagg tgaattactt cgacaattag aaaaacttcg
      241 ctgggaagct caagcaaatg gcaatgtcaa ttggtgtgaa gagcactcaa actactgtcg
      301 attcataaag gagactctct ataaaggaaa actcctttct tctcaacaga agcaagagtt
      361 agttttaata atggattatc taaaatcgtg tggtgaatat gctcaagctt atcaagaaaa
      421 tttgattgat gatgaggaat tagaaattga gaaattagca tatgttgatg acaatcttta
      481 tgatagagta ggggatatga ttgctttctt ttatcagaga acttaggtaa agaactaatg
      541 tttagttaag gatatcgcaa cagaaattgc tatataagct ctcttccgtt cccactactt
      601 aaggtgatta tcaaaaaagt aaaaaagaaa gaaaatttaa cttcccacca agaaacaaag
      661 aaaggaatgt tcggttagta gaaataactg aacacgggac tgaatcgtcc ctttgtatct
      721 taatttatgt caaacattca aaacatgtcc cttgaggaca tcatgggaga gcgttttggt
      781 cgctactcca aatacattat tcaggagcgg gccctgccgg acattcgtga cggtctcaag
      841 cccgtgcaac gtcggattct ttattctatg aataaggacg gcaacacttt tgacaagggt
      901 taccgtaagt ctgccaagtc agtcggaaat atcatgggga atttccaccc gcacggtgat
      961 tcctctattt atgatgccat ggtccgcatg agtcaggact ggaagaaccg cgagattttg
     1021 gtggagatgc acggaaacaa cggttccatg gacggcgatc cgccagcagc tatgcgttac
     1081 accgaggctc gcctttcgga aatggctggc tatctcttgg ctgacatcga gaaaaagacc
     1141 gtaccatttg cctggaactt tgacgatacg gaaaaagaac ccactgtgct accagctgcc
     1201 ttccctaatc tcttggtcaa tggagcgaca ggaatttcag cagggtacgc gactgacatc
     1261 ccgccgcata atctagcgga ggtcatcgat gccgttgtct acatgattga tcatccaaca
     1321 gctaagttag aaaagttgat ggagttcttg cctggaccag acttcccaac aggtgccatt
     1381 atccaagggg ctgacgaaat caagaaagcc tatgaaactg gtaagggccg tgtcgttgtc
     1441 cgtagccgtt gtgaaatcga gcaactcaag gctggtaaga aacagattgt cattactgag
     1501 attccgtatg aagtcaacaa ggctgttttg gtcaagaaga ttgatgatgt ccgtgttaac
     1561 aacaaggtgc ctggtattgc cgaggtgcgt gatgagtcag atcggacagg tctgcggatt
     1621 gccattgagc tgaaaaaaga tagcgatgag caaaccattc tcaactacct ctacaaatac
     1681 accgacctac aaatcaatta caactttaac atggtggcga ttgataattt cacaccgcgt
     1741 caggttggct tgcagaagat tctgtccagc tacatcgccc accgccgtga gattatcatt
     1801 gcccgttcta agtttgacaa ggaaaaagca gagaaacgcc ttcacatcgt agagggttta
     1861 atccgcgtcg tttccatttt ggacgaggtt attgccctta ttcgtgcttc tgaaaacaag
     1921 gcggatgcta aggaaaacct gaaaatcagc tatgagttca gcgaggagca ggcagaagca
     1981 atcgtgacct tgcaactcta ccgcttgacc aataccgaca ttgtgacctt ggaaaatgaa
     2041 gaagcagccc tacgcgagca gattcagacc ttggcagcta ttatcggcga tgagcggacc
     2101 atgttcaatc ttatgaagaa ggaactgcgt gaggtcaaga agcagttcgg caatcctcgt
     2161 ttgagtgaat tgcaggatca ggcagaaacg attgagattg atactgccag tctgattgtc
     2221 gaggaagaaa cttttgtcag cgtgaccaag gcaggttaca tcaaacggac aagtcctcgt
     2281 tctttcaatg cctcaacgct tgaagaaatg ggtaagcggg atgatgacca gctgattttc
     2341 ttgcagaatg ccaagacaac tcagcacctc ttgcttttta ccaatcttgg aaatgttatc
     2401 taccgacctg ttcatgagtt gacagatatt cgttggaagg acattggtga acacctgagc
     2461 cagactctca tgaactttga taccaatgaa gagattatct tcgctgaatt ggtagagaat
     2521 tttgaagagg ggacttattt cgcggtgacc aaatacggtc aaatcaagcg tgtggagcgg
     2581 aaggagttca cgccatggcg gacttacaag tccaagtcaa ccaagtatgc caaactcaaa
     2641 gatgcagagg atgtggttat tactgtatcg cctgtagtct tggatgacat catgctcatg
     2701 acagaaaagg gctacgctct gcgatttaac atcgaagaag tgccaatcat cggtgccaag
     2761 gcggccggtg tcaaggcggt taacctcaag gacgaggatg tggtggctgc ggcctttatc
     2821 agcaatacca actccgtcta tctcctgacc cagcgtggta gccttaagcg gatggcgacg
     2881 gaggaaatcc ctgtgaccag ccgtgccaag cgtggtttgc aggtgcttcg cgaactcaag
     2941 gctaagcctc accgtgtctt tgcggcaggt ccagtcttga cggatcaggg tgattttgat
     3001 ctctttagca cggcaaatga agatgatacg acaagtcaaa tccttcacgt gcagtccaaa
     3061 acaggcaaac tctatgaggt tgatgtgacc cagcttagcc tgtctgaacg cactagcaac
     3121 ggtagcttca tttcggatac catttccgat gaagaagtct tcagagcctg gatagactaa
     3181 aaaagatgaa aagattgctc gcaaagaagt ctttttgcta tactgtagag gaggtaaatg
     3241 ctatgaaaat tatcaaatta tttttcagta taaaagtcta tctatggctt gttctgtcct
     3301 tccttctgtt attgtgtggt gtatggggat acggctacat ctctggtgaa aagagtaatc
     3361 agcaggaagt gagtgtccat gcggctattg agtctgtcga gcaagtcaat gaattggtat
     3421 ttctaaatac agctgtccag aaaattgtga ccgctaaaaa ctacacggtc ttatttggtc
     3481 aagaaatgcc cttctcagaa aaatcagccc tcttaattat caagtacaaa gcaaaatttg
     3541 gtatcaaaag tcctgtatcc attgaacatc tttctgaaaa tcggtacaaa attacccttc
     3601 cagcctttga agtcattgga ttggaattag atgcagaaga accttatacc ttatatgata
     3661 agagtggtga gatactgagt tttgcgacag aagaaatcga tactgcccaa ctcatcacag
     3721 atgaatttca gtcggtagag caagaaaagt ttttagcaga ttatcaggaa gacataaaag
     3781 agtcagccaa aaactactat caaaatttat ttagtgctat ctcacctgaa attcaactag
     3841 aatttgtttt taaagagtaa gagtctggtt taccaggctt tttcttatat tcacaaaatt
     3901 ttctgaattt ttaattgaaa atcactttca aaagtagtaa aatattaaaa aatgaatttg
     3961 gagaaactat atgacagtga acattgactg ggaaaacctt ggtttttctt atatgaaact
     4021 accttaccgc ttcatcgctc atttcaaaaa cagccaatgg tctgaagggc agttgaccga
     4081 agatgcggaa ttgcacattt cagaaagctc accagccctt cactatggcc agcaggcttt
     4141 tgaaggcctt aaagcctatc gtaccaagga tggaaaactc cagctctttc gtccagacca
     4201 aaacgctgag cgcctgcaac ggacagctga ccgcttgctc atggagcctg taccgacgga
     4261 tcttttcatc aaggcttgta aagaagtggt caaagccaat gcagactacg ttcctcccta
     4321 tggaactggt gggacccttt atctccgtcc gctcctgatt ggtgtcggcg acatcatcgg
     4381 cgtcaaacct gcggaagagt acattttcac agtttttgcc atgccggttg gtaactattt
     4441 caaaggcggc ctggctccga ccaacttcct catccaagac aagtacgacc gtgcggcacc
     4501 aaatggaaca ggggcagcca aggttggtgg taactacgcg gcgtcgctct taccgggcca
     4561 atatgccaaa aagcagggct tctcagatgt catctacctg gatccagcta cccacaccaa
     4621 gattgaagaa gtggggtctg ctaatttctt tggtatcaca gctgataacg agtttgtcac
     4681 accgatttca ccatcaatct tgccgtctat taccaagtat tctctcttgt acttggcaga
     4741 gcatcgtctg ggtatgaagg ctgtggagcg tgaagtcctt gtcgaagaat tagaccagtt
     4801 tgtcgaagca ggagcttgcg gaacagctgc agttatctca ccaattggtg gtatccagat
     4861 tgcagacaaa ctccatgttt tccatagtga aacagaagta ggacctgtca ctcgtaaatt
     4921 gtatgaagaa ttaacaggta tccagtttgg tgatattgaa gcgccagaag gttggattgt
     4981 cgaagtagat taatttcaga aggcttgcac ttgcaagctc ttctcttttt ttgtagaata
     5041 gtaaaagtga ggtttgaaaa tgagtaaaaa agataaaaaa attgaaattc agattgaaga
     5101 tagcaaagtt cttgtcaata gagaagagtt ccctggctac cgccttgtta taggtaaaaa
     5161 agtaattgga gaaattgcag aattggctga aaataacttt gcagttatta aaaacggaaa
     5221 taccgaaagc ttttacaaaa aattagaaaa agcagtcgga aatattattg aaaattataa
     5281 tttaagtcac taaaagactt gtaatagtcg ctattttatg ttagaatagt gactgttagt
     5341 gtacggagag atagcgaaga ggctaaacgc ggcggactgt aaatccgctc cttcgggttc
     5401 gggggttcga atccctctct ctccattgaa caggatacaa aggacgttaa gaaatccgta
     5461 cgaaaatagg aaattgacgc agtgtgctaa acacacaggg aagtttatct ttttcactag
     5521 gattttagtc cgtgttcaac taagatacga gatatgttct ggtttaccag aaatttctgt
     5581 agaaaattag gagactgacg ctgagtgtta acactcaagg aaggctatct atttttctaa
     5641 gaaattaatc tcgagttcaa tttcttatga ttagtaaata aatgaattgt atctattttt
     5701 tggggtatcg ccaagcggta aggcaaggga ctttgactcc ctcatgcgtt ggttcgaatc
     5761 cagctacccc agtaaaggta ctagatatag tctagttcgt caaagatgtc taattgttgt
     5821 tgtccttgcg tgtgccttaa ataaaatata ttttatgttg ggtcgaaaga cctgattgtc
     5881 ggaggttttt ttataatgaa cgaatttgaa gatttgttga acagtgtaag cgaagttaca
     5941 cctggtgatg tggtaactgc agaagtattg acagttgatg ctggtcaagc aaacgtagct
     6001 atctcaggta ctggtgttga aggcgtattg actcttcgtg agttgactaa cgaccgtgat
     6061 gctgatatca acgaccttgt aaaagttggt gaaacacttg aattgttagt acttcgccaa
     6121 gttgttggta aagatacaga cactgttact tatcttgtat ctaaaaaacg tttggaagca
     6181 cgtaaagcat gggacaagct tgttggtcgt gaagaagaag ttgttacagt taaagtaact
     6241 cgtgccgtta aaggtggtct tgcagttgaa tttgaaggtc ttcgtggttt catcccagct
     6301 tcaatgatcg acagccgttt cactcgtaac actgagcgtt ttgtaggtca agaatttgat
     6361 gctaaaatca aagaagttga tcctgctgag aaccgtttca tcttgtcacg tcgtgacgtt
     6421 gttgaagctg cagctgcagc tgcacgtgct gaagtattcg gtaaattggc agttggtgaa
     6481 gttgtgactg gtaaagttgc acgtatcaca agcttcggtg cattcatcga ccttggtggt
     6541 gtagatggtt tggtacactt gactgaattg tctcacgagc gtaacgtatc tcctaaatca
     6601 gctgtaacag ttggtgaaga agttgaagtt aaagttcttg caatcgatga agttgaaggt
     6661 cgcgtatcat tgtcattgaa agctacacag cctggtccat gggatggcgt tgagcaaaaa
     6721 ttggtagctg gtgatgtaat cgaaggtaaa gtaaaacgct tgactgactt cggtgctttc
     6781 gttgaagtat tgccaggtat cgatggtttg gtacacattt cacaaatttc acacaaacgt
     6841 gttgaaaatc caaaagacgc tttgtcagta ggtcaagaag taactgttaa agttcttgaa
     6901 gtgaacgcag ctgatgagcg tgtatcactt tctatcaaag ctttggaaga gcgtccagca
     6961 gcagttgaag gtgaagaaaa agctgaaaaa cgtgctccac gtccacgtcg tcaaaaacgt
     7021 gaagaaaaac gtgattacga attgccagaa actcaatctg gtttctcaat ggctgatctt
     7081 ttcggtgaca ttgaattgta attcatataa aaacttgcct tgtgcaagtt tttcctttcc
     7141 acccttagtg taatggatat cacgtaagat tccggttctt gagatggggg ttcgattccc
     7201 tcagggtgga ctaaattgaa aagcctgcat taacaggctt ttttgttata ttatatcaag
     7261 gaggctatga tatgttacat tttgaaaatg actataacaa aggtgcccat cccgccttgc
     7321 tagaagcctt agttgcaagc aaccaagaag gtttatctgg atatggcact gataaatata
     7381 ctgaatctgc tatcgaaaaa atcagacttg ctacggactg tcctcatgca caagttacgt
     7441 ttttagcagg tggcacgcag accaatcaaa ttgtcatttc gtctatgtta gcttcctatg
     7501 aaggagtcat tgcagcggat actggtcata ttgcagttca tgaagcaggg gcaattgaat
     7561 ttacaggcca taaggtgttg gccttaccgc atacggatgg caaacttgtc gcaagtgaag
     7621 ttgagaacta tatttccgat ttctacgcag atgctaatca tgctcacatg gttcatccag
     7681 gaatggttta tatttcccat ccaacagagt atggtacact ttatagcaag tcagagttag
     7741 aagccttgtc aacagtttgt cgtcatcata acatcccgct ttttttagat ggagctcgtt
     7801 tgggttatgg tcttgctgca cgcgatacgg atcttgattt gaagacaatt gcagagctga
     7861 cagatgtttt ctacattggt gggacaaaac tcggtgcctt gatgggggaa gctttggtat
     7921 ttacaaagaa caaccagcct aagaatttcg tatcgattgt caaacaccat agtgccttgt
     7981 tggcgaaagg aagagtgaca ggtatccagt ttgattgctt atttactgat gacttgtatc
     8041 ttgaattggg gcgtcatgct atagcaatgg cagagcaact gacccagatt ttggaagaga
     8101 aaggctttca attctatctc aaatccccga ccaatcagca atttgttatt gttaagaatg
     8161 aagaattgac tagattgaca gaagttggaa ttgcctatgg cttctgggag aaatacgacg
     8221 atagccacag tatcatccgc tttgcgacca gctggtcaac tagtcaagcg gatattgatg
     8281 cgttaaggaa tagattataa ttaataaaaa agaaagggtt agcctttctt tttagtttgt
     8341 aaataatgat agcgataaac tgcataacgt tcaataaggc ggtagcctat tccccctgcg
     8401 attgcatgta caatcagggc aatgtgcaat tctagtggtg cttttatagt gataccaaat
     8461 agaataccga ttacagtcca tcctagtatc acccaccata agtacttaaa tggagcatac
     8521 tgaccatcct ggtctttaac ttcactggaa accctgattg aacaaccggt caatactccc
     8581 atggggagca atgaacgagt atcaaatggt tctggtaaaa atgacgataa ggcgaagatt
     8641 tgtataagcg caaaaatcag tagccaaacg aaaatcggcg tttttagaat gacgtatttc
     8701 atattgcaca cctcttgaaa ttagtatatc aaatccaaga atgtaaaaca aatatttttg
     8761 tgctattgat tagaagtcgt ttcggatgcc ccactagtta cctctgtatc agtactggtt
     8821 tctgtccttg tctccaaagc ttgggtagca gacgtttctg tagacgagga agagacagtc
     8881 gactctacta aagagctggt ctcggtttgg gttgaggatg ttgtttcgga gcttgaactt
     8941 gttgaatctt gtacttctgt taatggattg tcgcttgatg attggctggg gctagtggcg
     9001 ctggatgatg aaatggtagt acttggagat gttgaggatg ttgcaggttt tgctgtaaag
     9061 gtgtagatag tgatgcaggc agtgataatc cctaaaatag acccgataaa ggttaaatat
     9121 ttttgaaaat tagttaaacc agccggtttt ttatttgtat tcttacgtgt attcatgatg
     9181 tacctccacc tttagtttaa gataggaagt ttaaaactat cttaaagatt gccgaaaggc
     9241 atgaaaacat gataaaatag gactattgca ataaaaggaa tataaaaaat gatttatttt
     9301 gataatgcgg caacgacaca ggtctatcca gaagttctca agacctatac ggaagtggcg
     9361 acaaaaatct ggggaaaccc ctctagcctc catagtctag gcagtcaggc aacccgtatt
     9421 ttggaagcat ctcgcaagca aatcgcagaa ttgcttggcc aggatagcaa agaaatcttt
     9481 ttcacctcag gtggtacgga aggtgacaac tgggtcatca agggagtggc atttgaaaag
     9541 gcacacctgg gcaagcacat catcgtttcg gctatcgaac acccagccgt aaaagaatct
     9601 gcgctttggc tcaaaacgca aggttttgag gttgatttgg ctccggtcaa tgcccaaggt
     9661 tttgttgatg tttcagcctt agaaagcttg attcgtccag ataccacact ggtttctatc
     9721 atggccgtca acaatgaaat tggtgctatt cagcccattc aggaaatatc tcaactgtta
     9781 gctgataagc caaccatctc attccatgtg gatgcggtgc aggctatcgg caaagtgccg
     9841 acggaacggt atttgacaga ccgagttgat tttgccagtt tttcaggtca caaattccac
     9901 tctgtcagag gagtaggttt cgtctatatc aaagctggca agaaaatcgc tccgctattg
     9961 acagggggag gacaggaaag cgataaacgc tcaaccacgg aaaatgtggc tggcattgct
    10021 gcgacagcta aggcgctccg cttgaccttg gacaaggcag cagatagcca aaagcgactg
    10081 gcagccatga agcagatcct tgtggatgaa ttgggcaaat acgacgatgt gacagttttt
    10141 tctgggctag aggactttgt gcccagtatt gtaactttcg gaattaaaaa catccgtggc
    10201 gaggtcattg tccatgcctt tgaagaccac cagatttaca tttcgacaac ctcagcctgc
    10261 tcgtccaagg ctggaaaacc ggctggaacc ctaattgcta tgggggttcc gcagaagttg
    10321 gctcaaactg ccgtccgtat cagcttggat gatgacaacg atatggggca aatcgaacaa
    10381 ttcctcacga ttttcaaaca aatttatcaa aacacacaga aagtaaggta gaatgaacta
    10441 ttcagaaatt atgattcgct atggcgaatt gtcaactaaa gggaaaaaca agatgcggtt
    10501 tgtcaacaaa ctccgcaata atatcaagca tgtcctttct gtttacccag aagtgaccgt
    10561 ttattttgac cgtgaccgtg gtcatgtcta tttgaatggg gcggattatc aggaagtttc
    10621 agcttctttg aagaagattt ttgggatcca aaatttcgca ccgtcttata agattgaaaa
    10681 gtctgttcca gctttgaaag aagcggttgt ggaaataatg cagaccattt acaaggaagg
    10741 gatgaccttc aagattgctg cgcgtcgtag tgatcacagt tttgaattgg acagccgtga
    10801 ccttaaccaa gtccttggag atgcggtttt tacagctatt ccaaatgtac aggtacagat
    10861 gaagtcacca gatattacct tgcgagtgga aatccgtcct gatgctgctt atatttctca
    10921 tgaagaaatc aagggggcag gcggtctgcc agttggaaca tctggaaaag gaacgcttat
    10981 gttatcaggt ggtattgatt cgcctgttgc gggctattta gcactgaaac gtggggttga
    11041 aatcgaagcc ctgcactttg caagtccgcc ttatactagc ccgggtgcgc ttaaaaaagc
    11101 ccatgatttg actcgcaaat tgactgcctt tggtggtaat atcaccttta ttgaagtgcc
    11161 gtttacagaa attcaggaag aaatcaagga aaaggcacct gaagcttatt tgatgacctt
    11221 gacacgtcgc ttcatgatgc ggattacaga ccgagtacgg gaagagcgtg gtgccatggt
    11281 tattatcaat ggtgaaagtc taggacaagt ggcaagtcag accctggaat ccatgcaggc
    11341 tatcaatgcg gtgacaaata cacctgttat ccgtccagtc gttaccatgg acaagttgga
    11401 aatcattgat attgcacaag aaattgatac ctttgatatt tccatccagc catttgagga
    11461 ttgctgtacc atctttgccc ctgaccgtcc aaaaactaac cctaaaatca aaaatgtcga
    11521 gcaatacgag gctcgaatgg atgtagaagg cttggtggaa cgagctgtgg ctgggattat
    11581 cgtaactgaa atcacaccga aagaagaagt gaaagacgaa gtggatagtt taattgaaga
    11641 cttactgtag aattcaattg ttttggcata catttaaatc cagctattgc tggatttttt
    11701 aatacgaatc gttcggaaaa atcgaatttg tatgatataa taagacttca gaaaaaaaca
    11761 aaggagttat tatgaaaaaa ggagcactaa caggattact cctgtttggt atgttttttg
    11821 gtgcaggaaa cttgattttc ccacctgcac ttggtgtctt atctggtgag aatttctggc
    11881 ctgctatttt aggatttgtc gtttcgggtg tcggtattgc agtcattgcc ctgattgtcg
    11941 gaacattgaa ccccaaaggt tatgtacatg agatttctcg caagatttca ccgacttttg
    12001 caacgattta tcttgttgcc ttgtatttgg cgattggtcc tttctttgcc attccgcgta
    12061 cagctacaac gtcctttgaa attggtattg cgccattatt gggcgatgcg aatctcggtc
    12121 tttggttgtt tggctttaca gctctatact ttgtggcagc ttatctgatt gccctcaatc
    12181 catctcaaat cttaaatagt atcggacgta ttttaacccc tgtttttgcg attttgattg
    12241 tgattttggt ggtgcttggt attgttaagt acggatcaac aagtccgtta ccagcttcgg
    12301 aagcttactc agcaggacag gcatttggta caggatttat cgagggatac aatacactcg
    12361 acgcacttgc ttctattgct tttagtgtcg tagcagtaaa taccttgaaa caacttggtt
    12421 tctcaagcaa aagggaatat gtatcaacta tttggtctgt tggcttagtt gtagccttgg
    12481 cattctcagc tctatatgtt ggtttggctt ttttaggaaa tcattttcct gtgcctgcgg
    12541 atgttttggc ttccgatacc aacaagggtg tttacattct ttcgcaagca acacaggcta
    12601 tttttggacc aggtgcacaa attttcttag ctgctatggt tatcgttact tgctttacga
    12661 cgacggctgg cttgattgtt tcttcaggtg aatttttcgc tgagcgtttc ccacgtttta
    12721 gctacaaagt atatgcgact atctttacct tgattggctt tgggattgcc aacctcggtt
    12781 tgaacaatat catcaccttc tctgttccag ttcttttggt tctctatcca atcactatct
    12841 gtattgtctt gattaccatt gttaacaaat ttgtaccgct ttcgacttat ggtatgcaat
    12901 taactgtggg acttgtgaca gctttgtcat tggtagaagt gttagcagga caattcaact
    12961 ggactgctgt ttcaaaaatc atctcagctc tgccattggc aggacaatca ttagcatggt
    13021 tattaccagc tcttgtcgga atcgtcctct ctctattctt accaaacaag caggagagcg
    13081 aagtttttga aatgtaaaaa aatgatacaa aacacttgca tttgcaggtg tttttgttat
    13141 aataggactg ttgactatgc acacatcctg tgcaaccgca cgaatgccgt tctttcgttg
    13201 agagtagagt aagtggttcg tcccacggta ctaggcgagt cttcacatca aattcgggta
    13261 agacccaaaa atcataggag gtgcataatg agcacatatg caatcattaa aactggcggc
    13321 aaacaagtta aagttgaagt cggtcaagct atctacgttg aaaaattgaa cgttgaagca
    13381 ggtcaagaag ttactttcga agaagtagtt cttgttggtg gtgagaaaac tgttgtgggt
    13441 actccactcg tagcaggcgc tactgttgtt ggtactgttg aaaaacaagg taaacagaaa
    13501 aaagttgtta ccttcaagta caaacctaaa aaaggtagcc accgcaaaca aggtcaccgt
    13561 caaccttaca caaaagttgt tatcaacgct atcaacgctt aatcgcgtag cttgaagcaa
    13621 tcattacaga aattcggagg aataacatat gttaaacttg aatcttgcta acttgcaatt
    13681 tatggcccac aaaaaaggtg gaggttcaac gtcaaacggt cgtgactcac aagcgaaacg
    13741 ccttggtgcg aaagctgctg acggccaaac tgtatcaggt ggttcaatcc tttaccgcca
    13801 acgtggtaca aaaatctatc caggagctaa cgtaggtcgt ggtggagatg acactcttta
    13861 cgctaaagta gaaggcgttg tacgcttcga acgtaaaggt cgcgataaga aacaagtatc
    13921 tgtttatcca atcgcaaaat aagtctgaaa atggctttac aataggcttt ccgagttgtt
    13981 ttcggagagt cttttttgta atttgatttt gctattttta aaagtgtacg atagaagtga
    14041 tttaagacgg caatatcttc tctataggat aaaaatattt tgccatctgc actgttgcgc
    14101 tgcctataag ttctacgttc agtagtagat gaaatgttca gaggaagtgg tatgggttcc
    14161 aacttagtaa aattagtcat tgatgatttg gcgaacagaa attccaaagc ctttcaaatt
    14221 gcagttgaag aagagaaatt gggaacctgg aagttataca agaaattagt tttgaagaac
    14281 aggacgggct agtctatttg cgaaaacgca agagttcagc aaattcttgc tttttttgat
    14341 ataatggtag aagcagtttt aagaggtatc aggtatgaat attcaacaat tacgctacgt
    14401 tgtagccatt gcaaacagtg gtacatttcg agaggcggct gagaaaatgt atgtgtccca
    14461 gcctagtttg tccatttcca ttcgtgattt ggaaaaagag ttaggttttc aaatttttag
    14521 ccgaactagt tcaggaactt ttttgacaca aaaagggatg gaattctatg agaaagctca
    14581 ggctttagtt aagggatttg atcagtttga gcatctctat ttgcaacctg aggaaggtga
    14641 gaagactttt tcaatttcaa gtcagcatta tgattttctt cccccattga ttacggagtt
    14701 ctctcgtagc cacccgcagt accctcattt tcgcattttt gaatctacta cagtacagat
    14761 tttagatgag gttgctcaag gttatagcga gcttggtatt atttatttga acgagagaaa
    14821 tacaaaagga atcatgcaga aattggataa acttcacttg caagctgtag atttgtctgc
    14881 atttcagact catatctacc ttcgtgaaga tcatcctttg acaaagaaag ctgagattga
    14941 tccagtagat ttggtaggtc tacctaccgt acggttcact caagaaaaag aggcttatct
    15001 ttattattcc gagaacttga ttgatacgtc ggattcctcg gtggtgtttg atgtgacaga
    15061 ccgtgctact ttgaatggaa ttttggagcg tacggatgcc tatgcaactg gttcgggcta
    15121 tcttgatgac gagagtgtca atggcattac cgtaattcct ctcaaaggaa aggtagacaa
    15181 tcgcatggtc tacgttaaac gagctaatga tgaacttagt tcttgtgcca aagactttat
    15241 tgaaaccatg caggcatatt ttgataagaa gaaggatgaa aatgcgtaaa attggatttc
    15301 catttttgat ggtagtatta attggtttgg accagtttgt aaaagcttgg acagttgcca
    15361 atattgaatt agatacggta acggaattta taccaggatt gatgagctta gcttatctaa
    15421 gaaattatgg tgctgcctgg tcgattttac aaaatcagca gtggtttttc acgataatga
    15481 caattgttgc tgtgacaggc ctagtgtggt actatatcaa acagattaag ggcaatatct
    15541 ggactttatt cagtctgtct ttgatgattg ctggtgctct tggaaatttt attgatcgtc
    15601 ttagattggg atatgtggtg gacatgtttc acttggattt tattagtttc cctgtattta
    15661 atgtagcaga tgtttgtctg acagttggtg taggtatttt attt
//
LOCUS       DBNFCK010000035        15491 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.035, whole genome shotgun sequence.
ACCESSION   DBNFCK010000035 DBNFCK010000000
VERSION     DBNFCK010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 15491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15491
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.035"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(298..1257)
                     /locus_tag="AC1PIW_000896"
     CDS             complement(298..1257)
                     /locus_tag="AC1PIW_000896"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HIP6605849.1"
                     /translation="MSKKIIGIDLGGTSVKLAILTTEGEIQEKWSIKTNILDDGSHIV
                     PDIIDSIKQRFETHGLTKDDFLGIGMGSPGVVDSEAGTVIGAYNLNWKTLQLVKEQFE
                     SALGLPFFIDNDANVAALGEQWVGAGNNNPNVVFMTLGTGVGGGVIAAGNLIRGVKGA
                     GGELGHITVDFDEPFACTCGKKGCLETVASATGIVNLSRRYADQYAGDAKLKQMIDDG
                     QDVTAKDVFDLAKEGDDLALIVYRHFSEYLGVACANIAAVLNPAYIVLGGGVSAAGEF
                     LLDGVRKVFAENSFPQIKESTQIVLATRGNDAGVLGAASLVLK"
     gene            complement(1353..1688)
                     /locus_tag="AC1PIW_000897"
     CDS             complement(1353..1688)
                     /locus_tag="AC1PIW_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002934966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605850.1"
                     /translation="MKVLVMSYMVIYLLVTLGAALFSYLKTKKMNTLRLILTILSMIL
                     LTSTLYFYSQSYHDLQMVGFALGFTFISTLFLYNGTKEGSNFTTVMLFSIGRFILHIQ
                     FLILLYLFR"
     gene            complement(1689..3557)
                     /locus_tag="AC1PIW_000898"
     CDS             complement(1689..3557)
                     /locus_tag="AC1PIW_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIP6605851.1"
                     /translation="MSDFIVEHLTKSVGDKTVFADLSFIIHQGDRIGIIGVNGTGKTT
                     LLDVLSSRIGFDGDVSPFRTKNAYKIAYLTQEPDFDESKTVLDTVLSSDLRETQLIRE
                     YELLLSHYDETSQARLEKVMAEMDSLNAWEIESQVKTVLSKLGLTDLNKTVAELSGGL
                     RRRVQLAQVLLGNADLLLLDEPTNHLDIDTIEWLTTFLKNTKKSVLFITHDRYFLDNV
                     ATRIFELDRASLTEYQGNYQDYVRLKAEQDERDAALRHKKERLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKGDLANKIDDSELEINFETSRIGKKVINFENVSFSYPDKPILKD
                     FNLLIQNKDRIGIVGDNGKGKSTLLNLIAGDLQADSGKVDIGETIRIGYFSQTIKGLD
                     ESKRVINFLQEVAEEAKTTSGMVSIAELLEQFLFPRNTHGTLIEKLSGGEKKRLYLLK
                     ILLQRPNVLLLDEPTNDLDIATLTVLEHFLQGFTGPVITVSHDRYFLDKVANKILAFE
                     DSGIETFFGNYTDYLDEKAFLASSSAISLEKPKEKTEKIKENKKRMSYFEKQEWATIE
                     EDIAGLEERIAAIEAEMLTCGSDFTKLSDLQKELDEKNDLLLEKYERYEYLSKLEG"
     gene            complement(3550..4761)
                     /locus_tag="AC1PIW_000899"
     CDS             complement(3550..4761)
                     /locus_tag="AC1PIW_000899"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HIP6605852.1"
                     /translation="MKLNNLPSEFQEALPILEKIKAAGFEAYFVGGSVRDAILGRPIH
                     DVDIATSSYPQETKQIFSRTIDVGIEHGTVLVLEGKKEYEITTFRTEEEYVDFRRPSQ
                     VSFVRSLEEDLKRRDFTVNAFALDEEAQIVDLFDGMTDLENRTLRAVGIPAERFNEDA
                     LRIMRGFRFAATLDFEIEPTTFEAMVQTAPLLEKISVERSFIEFDKLLMADFWRKGLR
                     AMIDSKAYNFLPDLAGKSDELEAMLTSLTEEFRFSTSEQAWALLFVCLGIDNIKSFLK
                     KWKTSNDFQRSVVKLVEIYQLRQAGPVTKQICFKYGKEFLYLVEELHQAQGFVTDFAA
                     IDKIDQALTIHDKHEIVVNGGHLMKAFDLKPGPVLGELLKEVEFQIVEGQLENEEQAI
                     MTFVKGILENE"
     gene            complement(4758..5525)
                     /gene="dapB"
                     /locus_tag="AC1PIW_000900"
     CDS             complement(4758..5525)
                     /gene="dapB"
                     /locus_tag="AC1PIW_000900"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIP6605853.1"
                     /translation="MTIKVIIAGFKGKMGSTAVEMVKGDAALSLAALVDPFATETEVD
                     GVPVFKTKEEVASLEADVWVDFTTPKFTYENTRFALENGFAPVVGTTGFTPEEIEELT
                     ALSAEKGLGGLIAPNFAIGAILLMQFAAQAAKYFPNLEIIELHHDKKKDAPSGTAVKT
                     AELISQVRQSQTQGAADEEELIAGARGAAFDGFRIHSVRLPGLVAHQEVIFGAQGEGL
                     TIRHDSYDRISFMGGVNLGIKEVVKRSQLVYGLEHLL"
     gene            complement(5534..6385)
                     /locus_tag="AC1PIW_000901"
     CDS             complement(5534..6385)
                     /locus_tag="AC1PIW_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIP6605854.1"
                     /translation="MKLAVITDSSAVLNVQSIERDDLFVLSIPVSIDGENYIEGQNLT
                     VEEFYQKMASSKELPKTSQPSLMELEEILAKLTEKGYTHALGLFLSSGISGFYQNIQY
                     LADEFEGLTVAFPDTKITSAPLGMMVENVLKWADAGLSFEEITGKLDQEIGKTTAFIM
                     VDDLNHLVKGGRLSNGAALLGNLLSIKPILYFTGEGKIEVYEKVRTEKKAIKRLLEIL
                     QEQTTEGQYQIAIIHANAPQKAENFKRQLEEAGVGSDLPIVSFGSVIGTHLGEGAVAF
                     GISPIIE"
     gene            complement(6387..6761)
                     /locus_tag="AC1PIW_000902"
     CDS             complement(6387..6761)
                     /locus_tag="AC1PIW_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HIP6605855.1"
                     /translation="MEIIRDKEFVNQYHFDARNHAWEKENGIPETKLKVDFQLIEQNR
                     AENRTSMITILRFMIVLDHFVISGAMSQAVHLPNRLVEEPTEFTDEEKRVLVEPLLDI
                     LKRMTYEVTEIAFDAPGVNLEF"
     gene            complement(6857..8812)
                     /locus_tag="AC1PIW_000903"
     CDS             complement(6857..8812)
                     /locus_tag="AC1PIW_000903"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HIP6605856.1"
                     /translation="MKIQDVLRKDVMLLDLQATSKEAVIDEMIASLVDKGYVTDFDVF
                     KTGIMNREAQTTTGLGDGIAMPHAKNAAVKEATVLFAKSNKGVDYASLDGQPTDLFFM
                     IAAPEGANDTHLAALAELSKYLMKPGFADKLRGVSSPEEVISVFDAAEAADKTAEEVV
                     AAPSGDRPFIVAVTACTTGIAHTYMAEEALKKQAAEMGVDIKVETNGASGVGNKLTAE
                     DIKNAAGVIIAADKAVDMPRFNGKPLVSRPVAAGIKQPEELINLILDGKASAYTASEG
                     AATVESSEKLSLGKAFYKHLMSGVSQMLPFVIGGGILIALAFLFDGALGVPQDSLGSL
                     GSYHEIAAMFMKIGGAAFGFMLPLLAGYIAYSIAEKPGLVAGFVAGAIASSGLAFGKI
                     PYAAGGEETLALAGVSSGFLGALVGGFLAGGVVLVLRNALRNLPKSLQGLNAILLLPL
                     LGTAITGFLMFFVNIPMAAINTGMNNFLAGLEGSSAILLGLVLGGMMAVDMGGPVNKA
                     AYVFGTGTLAATVADGGSVAMAAVMAGGMVPPLAVFVATLLFKDKFTQEERNSGLTNI
                     VMGLSFITEGAIPFGAADPARAIPSFIAGSALAGALVGLSGIQLMAPHGGIFVIALTS
                     NPLLYIAYVLIGAVVSGVLYGALRKAK"
     gene            complement(8809..9720)
                     /gene="pfkB"
                     /locus_tag="AC1PIW_000904"
     CDS             complement(8809..9720)
                     /gene="pfkB"
                     /locus_tag="AC1PIW_000904"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HIP6605857.1"
                     /translation="MIYTVTLNPAIDYIVRLEDVETGSVNRMESDDKYAGGKGINVSR
                     VLKRLGYPNTATGFLGGFTGQFIKDGLIAEGIDTDFVQVDQDTRINVKIKADQETEIN
                     GLGPIVTDTQLAELEMVLAGLTKEDTVVFAGSAPASLGNEVYNRLIPVAKKAGAQVVC
                     DFEGQTLLDSLAHQPLLVKPNNHELEAIFNVELNGIADIETYAKKILEMGAQNVIISM
                     AGDGALLVTPPATYFAKPIKGTVKNSVGAGDSMVAGFTGEYVRSQDPIEALKWGVACG
                     TATAFSDDLASIEFIKETYEKVEVETL"
     gene            complement(9717..10466)
                     /locus_tag="AC1PIW_000905"
     CDS             complement(9717..10466)
                     /locus_tag="AC1PIW_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIP6605858.1"
                     /translation="MNILKSERKQIILERIHSQQFVRLEELIEVLDTSESTVRRDLDE
                     LENEGKLRRVHGGAEAPGNLQLEESILEKSVKNVQEKREIAESAARQIVNGDVIFLDA
                     GTTTGVLIDYLQQENLTVVTNSIHHAVRLVERKIKTIIIGGFVKQSTDASVGAVALEQ
                     IRQLNFDKAFLGMNGIDKNYFTTPDIEEATIKCTIIDNAKESYVLADASKIGQFSFVK
                     VAAIEKSNIICQSSESSLLDIIKEKTRVIEV"
     gene            complement(10568..11623)
                     /locus_tag="AC1PIW_000906"
     CDS             complement(10568..11623)
                     /locus_tag="AC1PIW_000906"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIP6605859.1"
                     /translation="MKRKVFEHYQPYKIKQKARFDLGNNENRIIDWAFLPQETLENLD
                     VNQLSFYGDNTYSELIEKYATYLGVNPKQVTVGVGSDHLIHMIVSTFMEKDDTFLTVN
                     PDFFMYETYNHMHGSRFEAIDLEEKNDTLVLPVEKLLARAEEVKAKIIMLSNPNNPSS
                     VAYSLEDLEKLAISFKGLLVVDEAYIEFANVESFISRLQQFDNVLVLRTLSKAFGLAG
                     LRVGFAVGSEELIYELDKVIPPFSLSNLTAKMAAVALNYTDKVLETVEVIQNLREEMI
                     LFLQGLDNCQVLPSQASFVTFKATWAEEFYQQALAQDWNFKYYPTGKLEGHIRLSIGR
                     PEEMDMMKKMIEKLVEA"
     gene            complement(11654..12379)
                     /gene="trmD"
                     /locus_tag="AC1PIW_000907"
     CDS             complement(11654..12379)
                     /gene="trmD"
                     /locus_tag="AC1PIW_000907"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIP6605860.1"
                     /translation="MRIDILTLFPEMFAPLEHSIVGKARDKGLLEINYHNFRENAEKA
                     RHVDDEPYGGGQGMLLLAQPIFDTMDSIEQTKPRVILLDPAGRTFNQAYAEELAQEEQ
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKEVSH
                     TDDSFSSGLLEYPQYTRPYEYRGMVVPDVLMSGHHENIRKWRLEESLRKTYQRRPDLL
                     ENYNFTAEELTIFEKIKAEDTVD"
     gene            complement(12369..12887)
                     /gene="rimM"
                     /locus_tag="AC1PIW_000908"
     CDS             complement(12369..12887)
                     /gene="rimM"
                     /locus_tag="AC1PIW_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIP6605861.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAKLALFDDK
                     DQFAMEVEIASHRKAKNFDIIKFKGMYHINDIEKYKGFSLKIAEENLTDLEDGEFYYH
                     EIIGLDVYENDILIGQIKEILQPGANDVWVVKRKGKKDLLLPYIPPVVLNIDIPNNRV
                     DVELLEGLDDEN"
     gene            complement(12985..14333)
                     /locus_tag="AC1PIW_000909"
                     /pseudo
     CDS             complement(12985..14333)
                     /locus_tag="AC1PIW_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730355.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
     gene            complement(14403..15386)
                     /gene="guaC"
                     /locus_tag="AC1PIW_000910"
     CDS             complement(14403..15386)
                     /gene="guaC"
                     /locus_tag="AC1PIW_000910"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HIP6605862.1"
                     /translation="MFNETPVFDYEDIQLIPNKCIINSRSEADTTVTLGKYSFKLPVV
                     PANMQTIIDEDVAEMLAKDGYFYIMHRFDEAGRIPFIKRMHEQGLIASISVGVKEYEY
                     EFVTSLKADAPEFITIDIAHGHAESVIKMIQHIKKELPETFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMVMIGSLFAGHIESPGKTIEVDGEKFKEYYGSASEYQKGAYKNVEGKKIL
                     LPAKGHLKDTLVEMEQDLQSSISYAGGRDITSLKHVDYVIAKNSIWNGDSI"
BASE COUNT         4507 a         3586 c         2950 g         4448 t
ORIGIN      
        1 taagaattag agcccccagt agagggagaa tcaacatggt tgctacgttt ccccagctag
       61 acgggaaaaa ccagtcacga atccagttat caaggaaaaa agcagataac aaaatcaata
      121 aaccaagaat ggtcagatag ttgattattt tagacatgtt tacctgtttc ataaggtgac
      181 actcctttct ctttacactt tcattgtatg cttaaatact acatttttca atatatgtat
      241 ataaagaaaa tgtgacagcg gaattgaaaa aagcagagtt tcccctgctt ttaacgatta
      301 tttcaagacg agtgatgctg cgcccaatac acctgcatca ttaccacgtg ttgccaagac
      361 aatttgtgta ctttccttga tttgtgggaa gctattttca gcaaagacct tgcgaacgcc
      421 atccaataag aactctcccg cagctgatac accaccacca agaacgatgt aggcaggatt
      481 gaggactgca gcaatgtttg cacaagcaac acctaagtat tctgagaagt gacggtagac
      541 aatcaaagcc aaatcatcgc cctcttttgc caaatcaaag acatctttag ccgttacatc
      601 ttgcccatca tcaatcattt gtttcagttt tgcatcgcct gcgtattgat cagcatagcg
      661 acggctgagg ttgacaatac ctgtcgctga agcaactgtt tctagacaac ctttcttacc
      721 acaagtacat gcaaatggct catcaaagtc aactgtgata tggcccaatt cgccaccagc
      781 acctttgaca ccacgaatca agtttccagc agcgatgaca ccgccaccga cacctgtacc
      841 aagcgtcata aagacaacgt ttggattgtt gtttccagca ccaacccatt gttcaccgag
      901 ggccgctacg tttgcatcat tgtcaataaa gaatggtaaa ccgagcgcag attcaaactg
      961 ctctttgacc aattgcaagg tcttccagtt gaggttatag gctccgataa cagtgccagc
     1021 ttcactatcc actacaccgg gtgagcccat accaattcct aagaaatcgt cctttgtcaa
     1081 accatgggtt tcaaatcgct gcttgatact gtcaataata tcaggaacaa tatgacttcc
     1141 atcatctaaa atatttgtct tgatagacca tttttcttga atttctccct cagtagtcaa
     1201 aatggccaat ttaacagaag ttcccccaag gtcaataccg ataatttttt tagacataat
     1261 catcacctca aatgtatttt ctttattata acacaatgca aaggtttgcg taaagccttt
     1321 tcaaaaattt gtacggataa aaattaattg ctctatcgaa aaagatagag caaaattaaa
     1381 aattgaatat gtaagataaa ccttccaatg gaaaagagca taactgtcgt aaaattacta
     1441 ccttctttcg ttccattata gagaaaaaga gtagaaataa aggtaaatcc cagagcaaac
     1501 cccaccatct gcaagtcgtg ataagactga ctataaaaat acaaggtgct tgtcagcaat
     1561 atcatggata ggatagttaa aatcaagcgc aatgtattca tttttttggt ctttagatag
     1621 ctaaacaaag ccgctcctaa ggtcaccaag agatagatga ccatatatga catgacaaga
     1681 actttcatct agccctccag tttgctcaga tactcatacc gctcatattt ttccaagagc
     1741 aggtcgtttt tctcatccaa ttccttctgc aaatcggaca attttgtaaa atcactacca
     1801 caggtcagca tttccgcctc gattgccgca atccgctcct ccaagccagc aatatcctct
     1861 tcgatggttg cccattcctg cttctcaaag taggacatcc gcttcttgtt ctctttgatt
     1921 ttctcggttt tctcctttgg tttttccaag gaaatggcag agctggaagc cagaaaggcc
     1981 ttctcatcca gatagtctgt gtagttgcca aagaaagtct ctataccgct atcttcgaaa
     2041 gccaagattt tattggctac cttgtctagg aaataacggt cgtgactaac ggtaatgact
     2101 ggaccagtaa agccctgcaa gaaatgttcc aagactgtca gagtcgctat gtccaagtcg
     2161 ttggtcggtt catctagtaa gagaacattg ggccgttgca agagaatctt cagcaaataa
     2221 agtcgcttct tctccccacc tgacaatttc tcaatcaggg taccatgggt attacgtggg
     2281 aagaggaatt gctccaagag ttctgcgata gaaaccatac cagatgtcgt cttagcttct
     2341 tctgccactt cctgcaagaa attgatgacc cgcttgcttt cgtccaagcc cttgatggtc
     2401 tgagaaaaat aaccgatacg gatggtttca ccaatatcta ccttaccgct gtctgcttgt
     2461 aagtcgcctg cgattagatt gagcagggta gattttcctt ttccattatc accaacaata
     2521 ccgatgcggt ccttgttttg aatgagcagg ttaaagtctt ttaaaatggg cttatcagga
     2581 taggaaaagc tgacattttc aaagttaatg acctttttac cgatacgaga ggtttcaaaa
     2641 ttgatttcca attcgctatc gtctatcttg ttggctaaat cgcctttgag gtcatggaaa
     2701 cgattgatac gagcctgctg cttggttgca cgagcttgag gttgtctgcg catccaagcc
     2761 agctcttgtt tgtagagccg ctctttctta tggcgaaggg cggcatctcg ctcgtcctgt
     2821 tctgccttca agcgaacata gtcctggtaa tttccctgat attcggttaa gctggcgcgg
     2881 tccaattcaa aaatccgtgt cgccacattg tccaagaaat agcgatcgtg ggtaataaag
     2941 agaacagact ttttagtatt tttcaagaaa gtcgtcaacc actcaatggt atcaatgtcc
     3001 aggtggttag tcggttcatc cagcaagagc aagtcagcat tgccaaggag gacttgggcc
     3061 aattggactc gtctacgtag accccctgat aactcagcaa cggttttgtt gaggtctgtt
     3121 agcccgagtt tcgacaagac agtcttgacc tggctttcaa tctcccaagc attgagagaa
     3181 tccatctcag ccatcacctt ttccagtctt gcttgactgg tttcatcgta atgagataag
     3241 agcagctcgt attcacgaat cagctgggtt tcacggaggt cagatgaaag aacggtgtct
     3301 aaaactgtct tgctttcatc aaaatcaggt tcctgggtca gataggcgat tttataagca
     3361 tttttggtac gaaaaggtga tacatctccg tcaaaaccga tacgacttga cagaacatcc
     3421 aacaaagtcg tctttcctgt accattgacc ccaataatcc cgatacggtc gccttgatgg
     3481 atgatgaaag atagatcagc aaagaccgtt ttatctccga cagatttagt caggtgttca
     3541 acgataaaat cactcattct ctaagattcc tttcacaaat gtcataatcg cttgctcttc
     3601 gttttccagc tgtccttcca caatctggaa ttccacctct ttgagtagtt ctcccaatac
     3661 tggacctggt ttgaggtcaa atgccttcat cagatgtcct ccattgacaa caatttcatg
     3721 cttgtcgtgg atagtcaagg cttggtcaat cttatcgatt gctgcaaaat ctgtaacaaa
     3781 accttgtgcc tgatgaagtt cctctaccaa gtataagaat tctttgccat acttgaaaca
     3841 gatttgcttg gtaacaggtc ctgcttggcg gagctgataa atctctacca acttgaccac
     3901 gctgcgttgg aaatcattgc tggttttcca ttttttcagg aaggacttga tgttatcaat
     3961 acctaggcag acaaagagca gggcccaagc ttgttcagat gtcgaaaagc gaaactcttc
     4021 tgtcagactt gttagcatgg cctctaattc atcacttttt ccagctaaat caggcaagaa
     4081 attgtaagcc ttggaatcaa tcatagcgcg caagcctttt cgccaaaaat ccgccatcaa
     4141 tagtttatca aactcgataa agctacgttc caccgagatt ttttccaaga gcggtgcggt
     4201 ttgcaccata gcttcaaagg tcgtcggttc aatctcaaag tccaaggtcg ctgcaaagcg
     4261 aaaacctcgc atgatacgga gggcatcttc gttgaaacgc tcagccggga tgcctacagc
     4321 ccgtagggtg cggttttcta agtcagtcat cccatcaaag aggtctacaa tttgagcctc
     4381 ttcatccagg gcaaaggcat tgactgtgaa gtcgcgacgt ttgagatcct cttctaagga
     4441 acgcacaaag gaaacctggc tcggcctacg gaagtcgaca tactcctcct ctgtccgaaa
     4501 agtcgtgatt tcatactcct ttttcccctc taatactagc accgtaccgt gttcaattcc
     4561 gacatcaata gtccgtgaaa agatttgttt cgtttcctga gggtagctgg atgtcgcaat
     4621 atcgacatcg tgaataggtc tgcctaaaat ggcatcccgc acggagccac caacaaagta
     4681 agcctcgaag ccagctgctt taattttctc taaaatcggc aaagcctcct gaaactcaga
     4741 aggcagattg tttaatttca taataagtgt tccaaaccat aaacgagttg tgagcgtttg
     4801 actacctctt taatgccgag attgacaccg cccataaagg aaatacgatc gtaagagtca
     4861 tgacgaatgg tcaatccctc gccctgtgcc ccaaaaatca cttcctgatg ggcaacaagg
     4921 cctggcaagc gtactgagtg gatacggaaa ccgtcaaatg ctgcaccgcg agcgccagca
     4981 atcagttctt cttcatccgc tgcaccttga gtctgtgact gacggacttg tgaaatgagt
     5041 tcggccgttt tgacagccgt accactcggt gcatccttct tcttatcgtg gtgtaattca
     5101 atgatttcaa gatttgggaa atacttggct gcctgtgctg caaattgcat gagcaagata
     5161 gcgccaatag caaagttagg agcaatcaag ccccccaagc ccttctcagc tgacaaggct
     5221 gtcaattcct cgatttcttc aggggtaaat cctgtcgttc caaccacagg tgcgaaacca
     5281 ttttccaagg caaaacgggt gttttcatag gtaaattttg gcgttgtaaa atccacccag
     5341 acatcagctt ccagactggc aacttcttct ttggtcttga aaacaggaac accgtctact
     5401 tctgtttctg tcgcaaatgg atctaccaag gcagccagac taagtgctgc gtcacctttg
     5461 accatctcaa cagccgttga gcccattttt cctttgaatc ctgcgataat taccttaatt
     5521 gtcatgcaaa ctcctattca ataatgggag aaataccaaa ggccactgcc ccttctccca
     5581 agtgcgtacc gatgaccgaa ccaaatgaca cgattggtaa atcgctaccg acacctgctt
     5641 cttccaactg ccgtttaaag ttttcagcct tctgaggtgc gttggcgtgt atgatagcaa
     5701 tctgatactg tccttcggtc gtttgttctt ggaggatttc caagaggcgt ttaatggcct
     5761 tcttctccgt ccgaactttt tcatacactt ctatcttacc ctcacctgta aaatacagaa
     5821 tcggtttgat gctcaaaaga ttgccaagca aggctgcacc attggacaag cgaccacctt
     5881 ttaccaaatg attgaggtca tcaaccatga tgaaggctgt agtcttccca atttcttggt
     5941 ccaatttccc agtaatctct tcaaaactaa gtcctgcatc agcccatttg agaacatttt
     6001 cgaccatcat acctaaagga gccgatgtga ttttggtatc tggaaaggca acggttaatc
     6061 cttcaaattc atcagccaaa tattgaatat tttgataaaa acctgaaata cctgatgaca
     6121 agaataagcc aagtgcgtgt gtatagcctt tctctgttaa tttagccaga atttcttcta
     6181 gttccatcaa actcggttga cttgttttag gtaattcttt cgatgaagcc attttttgat
     6241 aaaactcttc aaccgtcaaa ttttgtcctt cgatataatt ttccccatcg atgctgactg
     6301 gaatacttag gacaaacagg tcatcccgct caatactttg aacattcaaa acagccgatg
     6361 agtcagttat aaccgctaat ttcatattaa aactccaaat tcactcctgg tgcatcaaag
     6421 gcgatttcag taacctcata agtcattcgt tttaaaatgt ccaaaagagg ctctaccaaa
     6481 acacgttttt cctcatcagt aaattcagtt ggctcttcta ccaatcgatt tggtaagtga
     6541 acggcctgac tcatggcacc tgaaatcaca aaatgatcta gtacaatcat aaagcgcaaa
     6601 atggtaatca tagatgttcg attttctgca cgattttgct caatcaattg aaagtcaact
     6661 ttcaattttg tttccggaat tccgttctct ttttcccacg cgtgattgcg ggcatcaaaa
     6721 tgatactgat tgacaaattc tttatcgcga atgatttcca tttcaagact cctctaataa
     6781 caataatact cacattatac cataatcctc gtgaaatagc taaaaaacct tgccagaagc
     6841 aaggtttcaa tcaaaattat ttagctttac gaagtgctcc gtaaaggaca cctgatacaa
     6901 ccgcaccaat caaaacatag gcgatgtaaa gcaatggatt agatgtcaaa gcgataacga
     6961 agataccacc gtgaggagcc atcaattgga tgcctgacaa accaaccaag gcacctgcaa
     7021 gggcagaacc agcgataaag cttgggattg cacgagctgg gtcagcagca ccaaatggaa
     7081 tcgcaccttc agtgatgaat gaaagaccca taacaatgtt tgtcaaacct gagttacgct
     7141 cttcttgtgt gaatttgtct ttgaaaagaa gggttgcaac gaatactgcc aacggtggaa
     7201 ccataccacc tgccattacc gctgccatgg caacagaacc accatctgca acagttgctg
     7261 caagtgtacc tgtaccgaag acataagctg ccttgttgac tggaccaccc atatcgactg
     7321 ccatcattcc accgaggaca agtccaagga gaatagcaga gctaccttca agacctgcaa
     7381 ggaagttgtt catacctgtg ttgattgctg ccattgggat gttaacgaag aacatcaaga
     7441 aaccagtgat tgctgttccc aaaagtggca agagcaagat agcgttcaaa ccttgaagtg
     7501 attttggaag gttgcgcaaa gcgttgcgaa gaacgagcac tacaccacca gcaaggaaac
     7561 caccaacaag ggcacccaag aaaccagatg atacacctgc aagagcaagc gtttcttcac
     7621 caccagctgc gtaaggaatt ttaccgaacg caagaccgct actagcaatt gcaccagcta
     7681 cgaaacctgc aaccaaacct ggtttttcag caatcgagta agcaatataa cctgctagaa
     7741 gtggcaacat gaagccgaag gctgctccac caatcttcat gaacatagct gcgatttcat
     7801 ggtatgaacc aagactacca aggctgtctt gtggcacacc tagagctcca tcgaatagga
     7861 aggcaagggc aatcaagata ccgccaccga taacgaatgg caacatttga gacacaccac
     7921 tcatcaagtg tttatagaac gctttgccaa gactgagttt ttctgaagat tccacagtag
     7981 ctgctccttc tgacgcagtg taggctgatg ctttaccatc caagataagg ttaattaatt
     8041 cttctggttg tttgattcca gctgcaactg gacgagatac caaaggtttg ccattgaagc
     8101 gaggcatatc aacagcctta tctgctgcga tgataacacc tgctgcattc ttaatgtctt
     8161 cagcggttag tttgtttcca acacctgaag caccgttggt ttcaaccttg atgtcaacac
     8221 ccatctcagc agcttgtttc ttaagtgctt cttctgccat gtaagtgtgg gcgatacctg
     8281 ttgtacatgc tgtcacagca acgataaatg gacggtcacc acttggtgct gcaacaactt
     8341 cttcagcagt cttatctgct gcttcagctg cgtcaaaaac tgaaatgact tcttctgggc
     8401 tagacacacc acgaagtttg tcagcaaatc caggcttcat caagtattta gacaattcag
     8461 caagggcagc caagtgtgta tcgtttgcac cttctggagc tgcaatcatg aagaacaaat
     8521 cggttggttg accatcaaga cttgcgtagt caactccctt gtttgatttt gcaaagagaa
     8581 cagttgcctc ttttacagcc gcattcttag catgtggcat tgcgataccg tcgccaagac
     8641 cagtagttgt ttgcgcttca cggttcatga tacctgtttt gaaaacatca aaatctgtaa
     8701 catatccttt atcaaccaaa ctagcaatca tttcatcgat aactgcttcc ttgcttgtcg
     8761 cttgcaaatc aagcaacatg acatcttttc tcaaaacgtc ttgaattttc atagtgtttc
     8821 tacctcaact ttttcataag tttccttaat aaattcgata cttgccaaat catctgaaaa
     8881 ggctgttgcc gtaccgcaag caactcccca ttttagggct tcaattgggt cttgtgagcg
     8941 aacgtattca cctgtaaaac ctgccaccat ggaatctcca gctccgacag agtttttcac
     9001 tgtacccttg attggtttag caaagtaagt agctggtgga gtgaccaaca aagccccatc
     9061 ccctgccatg gaaatgatca cattctgagc acccatttct aagattttct tagcgtaggt
     9121 ttcaatgtct gcaatgccat tcaattcgac gttgaagatt gcttcaagtt catggttgtt
     9181 tggcttgacc aaaagtggct ggtgtgctag tgagtctagg agtgtttgac cttcaaagtc
     9241 acagacaact tgcgctcctg cttttttcgc cacaggaatc agcctattgt agacttcgtt
     9301 tccaagacta gctggagcag acccagcaaa gacaacggtg tcttctttgg tcaatcctgc
     9361 taagaccatt tctaattcgg ccaactgtgt atctgtcaca atcggaccca aaccgttgat
     9421 ttctgtttct tggtcagctt tgattttcac gttaatacgt gtatcttgat caacctgaac
     9481 aaaatcagta tcaattcctt cggcaatcaa tccatctttg atgaactgac ctgtgaatcc
     9541 cccaaggaaa cccgttgctg tattcggata gcccaagcgc ttcaaaacac ggctaacgtt
     9601 aattccctta ccgccggcat acttatcgtc gctctccata cgattgacac tgcccgtctc
     9661 gacatcttct aaacgaacga tatagtcaat cgctggattg agcgtaactg tataaatcat
     9721 acctcgatta ccctcgtttt ctcttttatg atgtccaaca aactgctttc tgaagattgg
     9781 cagataatgt tggatttttc gattgccgca actttaacaa aggagaattg accaatttta
     9841 gaagcatctg ccaaaacata gctttctttg gcattgtcta tgatagtgca tttgatggtc
     9901 gcttcttcaa tatctggcgt tgtaaaatag tttttatcaa taccattcat ccccagaaat
     9961 gctttatcaa aattgagctg tcgaatttgt tccaatgcta ctgctccaac ggaagcatct
    10021 gttgattgct taacaaaacc acctataatg attgttttta tcttgcgttc aaccagcctc
    10081 actgcgtggt gaatagaatt ggtaacgact gtcagatttt cctgctgaag ataatcaatc
    10141 agtacaccgg ttgttgtacc tgcatctaaa aaaattacgt ctccgttaac aatttgtcga
    10201 gcagcacttt ccgcaatctc tcgtttttct tgaacgtttt tgacagattt ttctagaata
    10261 gactcttcga gttgcaaatt ccctggcgct tcagcaccgc cgtgtacacg acgaagctta
    10321 ccttcgtttt ccaactcatc caaatcccta cgtacagtag actcagatgt atcaagaact
    10381 tcgattaatt cctctagccg aacaaactgt tgagagtgta ttcgctctaa tataatctgt
    10441 tttcgttctg atttgagtat gttcaacacc tcctgcaacc gtttacaaaa atcattatac
    10501 acgattttct gccattgtca atcattttct atcattttct ttcaaaaaac aaaaaaacta
    10561 gcagaattta cgcttccact agtttctcaa tcattttttt catcatgtcc atttcttctg
    10621 gacggccgat agacaggcga atatgccctt ctaatttacc tgttggataa tatttgaaat
    10681 tccaatcctg tgccaaggct tgctgataaa attcttctgc ccacgttgct ttaaacgtta
    10741 caaaactagc ctgactaggt agaacttgac agttatctaa cccttgcaag aataaaatca
    10801 tttcttctcg caaattctga attacttcca ctgtttctaa aaccttatct gtataattca
    10861 atgcaacggc tgccattttt gcagtcaagt ttgacaagct gaatggggga atgaccttgt
    10921 ccaattcata aattaactct tcgcttccta ccgcaaagcc aactcgtaaa cctgcaagtc
    10981 caaaagcctt tgataaagtc cgtaaaacta aaacattgtc aaattgttgc agacgactga
    11041 tgaagctttc gacatttgca aattcaatgt aagcctcatc tacaacgagt agccctttaa
    11101 aagaaatagc caatttctcc aaatcttcta gtgaataagc tactgaagaa ggattgttag
    11161 gattggaaag catgattatc tttgccttca cctcctccgc acgcgccaac aatttctcta
    11221 ctggcaggac gagtgtatcg tttttttctt ctaaatcaat ggcttcaaaa cggctaccat
    11281 gcatgtgatt ataggtttca tacatgaaga agtctggatt gacagtcaaa aaagtatcat
    11341 ccttttccat aaaggtggaa acaatcatgt gaataaggtg atcagagccc acacctaccg
    11401 tcacttgttt gggattaact ccaagatagg tcgcatattt ctcaatcagt tcggagtaag
    11461 tgttatcacc ataaaaactt aattgattga catccaaatt ttccaaagtc tcttgtggca
    11521 aaaaagccca atcaatgata cgattttcgt tatttcctaa atcaaatctt gccttttgtt
    11581 tgattttata aggttggtaa tgttcaaata ccttgcgttt catcatttct ccttctagca
    11641 agaaaaacac agtctaatca actgtgtctt ctgctttgat tttttcaaaa atagttaact
    11701 cttcagctgt aaagttgtag ttttctaaca agtctggacg acgttgatag gttttgcgta
    11761 gactctcttc caaacgccat ttacgaatat tttcatggtg accgctcatt aatacatctg
    11821 gaactaccat tccacgatac tcgtatggtc gggtgtattg aggatattcc aatagccctg
    11881 atgaaaaact atcatccgta tgactgactt ccttgccaat aacctctgga atcaggcgaa
    11941 cggtagcatc aatcatggtc atagctgcca actctccacc tgtcaggaca taatctccga
    12001 gagaaatttc atctgttact aaggtcttaa tccgctcatc atatccttca tagtgaccgc
    12061 aaatgaaaat cagctgctct tcctgtgcca attcttcggc ataggcctga ttgaaagtac
    12121 gcccagcggg atccaatagg ataacacgtg gcttggtttg ttcaatgcta tccatggtat
    12181 caaaaatcgg ctgggcaagg agcaacatac cttgtcctcc gccatagggt tcatcgtcta
    12241 catggcgagc cttttcagca ttctctcgga aattgtggta gttgatttct agcaaaccct
    12301 tgtcacgcgc cttacccaca atagagtgtt ctagaggtgc aaacatctca gggaagaggg
    12361 tcaaaatatc aattctcatc gtctaggccc tcgagcaatt ccacatcgac acggttattt
    12421 ggaatatcaa tgttcaagac aacaggtgga atatatggca agagcaggtc tttcttacct
    12481 tttcgtttca ccacccagac atcattggca cctggctgca agatttcctt gatttgtccg
    12541 atcagaatat cattctcata gacatctaag ccgataattt catggtaata aaactcacca
    12601 tcttcaaggt cggtcaaatt ttcctcggca attttcaaac taaagccctt gtacttttca
    12661 atatcgttga tatggtacat gcccttaaac ttaataatat caaagttctt agccttacgg
    12721 tgactagcaa tttccacttc catggcaaac tgatccttgt catcaaaaag tgcaagcttt
    12781 gccccttttt taaaacgttc ttcagcaaaa tcggtcacag acaaaacacg catttcccct
    12841 tgtaggcctt gcgtgttaac aatctttcct acgttaaaat aattcataat ttctccattt
    12901 gattttctat ccttttattt taacatgagc aagatatttt cacaagagag aaaaagccgg
    12961 atgcctccga ctttcatagt taatttattt cacagacaaa tgagaatgag ctattttgat
    13021 caagcgcaaa ttgagagcta ttccgcagac aaaaatccct gaaataagac cgatccaata
    13081 ggcttctgga cccatttgga aaaatctttc aagcaagaat gcgactggga aggtcatgga
    13141 ccaataggca accaaaccaa gtataaatgg aacagtcgta tccttgtaac cacgtaaaat
    13201 cccctgaata ggtgctgtaa aggcatctgc taactggaac atgagggcaa agctcaggaa
    13261 atgcgaggtc agacggataa attctgcgtc attaccataa aggtaggcaa ctttggagcg
    13321 gaaaaaatat aggaaagtta gcgtcaaacc tgcaaagcct agtgctacta agcgtcccag
    13381 acggctgtag gctttcacat cttgtggtcg tttagcgcct acttcataag caatgacaat
    13441 agctagggct gaggataccg atatggaaag gcatataata aggttgcaaa gttcatagct
    13501 gactggtggg ctgctataat ctgtgctgaa aattttgcca tgtagagccc tactactgca
    13561 aaaattgcta cttctgcaaa gacttgtaaa ccgatgggaa gaccgatttt cagtccttct
    13621 ttcagtaaag acccatcaat cggactccac cgccaaatct gataagaaga aatagtctta
    13681 tttttgcaca tgacaataac gattactagc aataccgccc agtaagctaa agcagtaccc
    13741 aatccagctc cagctccgcc tagggccggg aggcccattt tcccataaat taagagatag
    13801 ttaaatagac tgttaaatgg gacaaggagc aacatgaggt acatggatag ctgagtcaag
    13861 ccgagggcgt cgaaaaaaga gcgacagaca ctaaagagca agagaggcag gataccaatg
    13921 gaaataaaat agaggtactg ccgaccaact tgaaaaacag attcatcaag tccaaactga
    13981 gccaagacgg gaacagcgat aaataggatc gagcacaaga gtaaaatggt taatccaata
    14041 gatagataga caaactgatg aaactcttgt cgaatctttg acttctctcc ttgtcccaaa
    14101 tattgggcaa taatcgagac caatgcagat acaatccctg taagaaatga aaaaagcgga
    14161 ttccaaagac ttgttgccat ggaaacacct gccaaatcca tagtactata ctgaccagtc
    14221 atcatggtat cgataaagga tgcggagtaa ttagcaaatt ggtaaattaa aatgggcaaa
    14281 aatattttga taaagagcca gagcttctct ttgttatttc ttgtttggta catacttatt
    14341 ctctttcaat tttatataaa tattgtaaca agaaaaggac ctgcattgca agtcctcttt
    14401 gactagatag agtcaccatt ccagatagag tttttcgcga taacatagtc cacatgcttc
    14461 aagctggtaa tgtcacgtcc tcctgcataa gaaatagatg actgcaaatc ttgctccatt
    14521 tcaaccagag tatcttttaa atgtcctttg gcagggagta agattttctt accttctacg
    14581 tttttataag cacctttttg gtattcagat gctgaaccgt agtactcttt gaatttctca
    14641 ccatcaactt caattgtttt acctgggctt tcaatgtgac cggcaaagag cgaaccaatc
    14701 atgaccatgc ttgcaccgaa gcggatggat ttggcaatat ctccgtgggt acgaatacca
    14761 ccgtccgcaa taattggttt gcgggcagct tttgcgcacc aacgaagggc agccaactgc
    14821 caaccacctg taccaaaacc agtcttaacc tttgtaatac atactttacc tggaccaata
    14881 ccaacttttg tcgcatctgc accagcattt tctagttcac gaactgcttc tggagtaccg
    14941 acattacctg caataacaaa tgtctcaggc agttctttct tgatatgttg aatcatcttg
    15001 ataacacttt ctgcatggcc atgagcaata tcaatggtga taaactcagg ggcatcagct
    15061 ttcaagctag tcacaaactc atattcatat tcttttacac caacagagat agaagcaatc
    15121 aaaccttgct cgtgcatgcg tttgataaat ggaatacgac cagcttcatc gaaacgatgc
    15181 atgatgtaga aataaccatc tttggcaagc atttctgcca catcttcatc aataatagtc
    15241 tgcatattag ctggaacaac tggtaattta aagctatatt tcccaagagt cactgttgta
    15301 tctgcctcgg aacggctatt gatgatacat ttatttggaa tgagctgaat atcttcgtag
    15361 tcaaaaactg gagtttcgtt gaacatgctt ctacctcttt ttcttaacat tggaacagaa
    15421 aaacggtttg tttttcgtat tttttcctta ttacaagata tagtatagct tattgtttgt
    15481 gttttgtcaa t
//
LOCUS       DBNFCK010000036        15405 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.036, whole genome shotgun sequence.
ACCESSION   DBNFCK010000036 DBNFCK010000000
VERSION     DBNFCK010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 15405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15405
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.036"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(78..533)
                     /locus_tag="AC1PIW_000911"
     CDS             complement(78..533)
                     /locus_tag="AC1PIW_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIP6605863.1"
                     /translation="MELHSHFNAENQTAFEHVIQHLKEKGIRITETRKAVVAYIIESD
                     DHPSAEMIYQDLIPNYPNMSLATVYNNLKLLLEEGFVTKLKRTNDNTTYYDFMGHEHL
                     NVICEVCGKITDFMDVEIPSLKKEAHTQTGYKITKEVLSIYGICPDCQN"
     gene            700..1449
                     /gene="truA"
                     /locus_tag="AC1PIW_000912"
     CDS             700..1449
                     /gene="truA"
                     /locus_tag="AC1PIW_000912"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIP6605864.1"
                     /translation="MTRYKAIISYDGHDFSGFQRQPHARTVQEEIEKTLVRLNSGQPV
                     TVHGAGRTDAGVHAYGQVIHFDLAGSRDVEKLRFALDTQTSEDIDVVSVEQVADDFHC
                     RYAKHSKTYEFLVDIGRPKNPMMRHYATFYPYDLDLSLIEEAIQDLVGTHDFTGFTAS
                     GTSVEDKVRTITVASMEYDQQRQFLIFTFSGNGFLYKQVRNMVGTLLKIGNGRMPVGQ
                     IKRILAEKDRGLAGPTAAGNGLYLKEIIYED"
     gene            1439..2200
                     /locus_tag="AC1PIW_000913"
     CDS             1439..2200
                     /locus_tag="AC1PIW_000913"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004184219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HIP6605865.1"
                     /translation="MKTKYILALSGNDIFSGGGLHADLTTYTVHGLHGFVAVTCLTAM
                     TEKGFEVIPTETAVFAQQLASLKDVPFSAIKIGLLPSVEIAEQALEFIKTHQEIPVVL
                     DPVLVCKETHDTEVSQLRDEILKFLPYVSIITPNLAEAQLLLQKDIKTVEEMKQVAVV
                     LYDLGAKAVVIKGGNRLGGSEAVDVFYDGQEVVVLQSPLLSENNVGAGCTFASSIASQ
                     LLLGQEPLEAVRLSKDFVHAAIEKSDDYGVVQYEK"
     gene            2190..2675
                     /locus_tag="AC1PIW_000914"
     CDS             2190..2675
                     /locus_tag="AC1PIW_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIP6605866.1"
                     /translation="MRNKQTQELVLLAILTALSLVLAFVHVPTATGFVTLLDVGVYFT
                     AFYLGKKEGAIVGGLSGLLIDFLLGYPQWAFFSLVFHGAQGYFAGWTGKKRILGLVLA
                     TLSMVGGYYLASRLYFNDLKAIESVLSNTLQNTVGLVVGYVLAQAIERIGAVNHVIKG
                     N"
     gene            2656..3222
                     /locus_tag="AC1PIW_000915"
     CDS             2656..3222
                     /locus_tag="AC1PIW_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HIP6605867.1"
                     /translation="MLLREIKDQTKQVVQEVLELSDLQKGQIFVLGLSSSEVIGGHIG
                     KNSSLEVGEVVVETILEILDQKGIYLAVQGCEHLNRALVVERELAIQKDLEIVNVLPT
                     LHAGGSGQLAAFKYMKDPVEVEFITAQAGVDIGDTAIGMHIKHVQVPIRPSLREIGQA
                     HVTALASRPKLIGGVRAAYQEDEIRKGS"
     gene            3222..3836
                     /locus_tag="AC1PIW_000916"
     CDS             3222..3836
                     /locus_tag="AC1PIW_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1349 domain-containing protein"
                     /protein_id="HIP6605868.1"
                     /translation="MKEFTVKDLVWTRPPRQFTISDEEVTITTHPQTDLWQKTYYHFV
                     NDNAPVLQMETEEDYFSFTVKTDFAGSHTRFDQCGIVLYQDSENWLKCSIEYENDDFQ
                     HLGSVVTNHGFSDWATTEVSSQIKSMWFRLSRRGADYCFENSEDGIHFKQMRVCHFHQ
                     GAGKIAFGLYACSPENSSFTARFTNMELSDCKWQAHDGQEPDEI"
     gene            4109..4873
                     /locus_tag="AC1PIW_000917"
     CDS             4109..4873
                     /locus_tag="AC1PIW_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605869.1"
                     /translation="MTKGYQQRFSLSILLWMRQDKARQEGMDYWSGGHAQIIAASPGL
                     LEYRQQHLSETEHSFWPKATGLETAVPEDRRIDGIAEVTYQKLLAPIGGRRQTALAFE
                     DEVNVFRRTLMHMGFPYSSRWYHTSTQAQTQLRDVLYFRRKDGVKSGSFKKFVQDGLA
                     SQLATISGVTEVRTQVYLPWNKATWNTPNVAHDNPKEDHLHASIIPGFADQAAREAFY
                     ANQAPQLNAEVVQYSSAVHAYHIEKTLPFVLDGKRM"
     gene            5154..5585
                     /locus_tag="AC1PIW_000918"
     CDS             5154..5585
                     /locus_tag="AC1PIW_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HIP6605870.1"
                     /translation="MDTKFSVALHILTMISESKEILSSQALAESVGTNASYIRKVIAL
                     LKNAGLITSHQGRSGYQLSKSPKDISLLEIYLATQEVEHIRLFQIHQNANLSCPVGQH
                     IEGAMVPIFSSVEQELENQLSRQSLDNVITNLYQSAQVTRI"
     gene            5665..6666
                     /locus_tag="AC1PIW_000919"
     CDS             5665..6666
                     /locus_tag="AC1PIW_000919"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIP6605871.1"
                     /translation="MKAAQHTSYNKNNIALNLTEIAKPQIKPNQVLVKVTAAGVNPLD
                     NMISRGDVKLIVPYKLPQTAGNEVVGLVEEVGSSVTTFAAGDRVFGRLPLDSIGAFAE
                     YVAVDAQALAKVPAYLTDEEAAAVPLTALTIMQALDLMGAQSGKTIFISGGTGGVGGM
                     AIPIAKAKGLTVITNGDGASAERVLSLGADRFIDYKTEDYTKTLSDVDYVLDTLGGAE
                     TEKQMSIMKKGGQLVSLRAMPNGEFAKRMNLPKWKQILFGLAGRSFDKMAKKYGVHYH
                     FIFVESNGRQLQEVADIFNKLEIKPSIDTVYPFEEVNAALDKVANGRSRGKTVLSFN"
     gene            6692..7537
                     /locus_tag="AC1PIW_000920"
     CDS             6692..7537
                     /locus_tag="AC1PIW_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIP6605872.1"
                     /translation="MSYITTKNQYITVSNNKIAYRELSKGKSDIPLVMLVHLAATLDN
                     WDPKLLDLLAEKHHVIILDLPGVGASQGKVAPTIPGMAEQAVAIIKALGHEKINLLGL
                     SMGGFIAQEIVRLDSQLVNRLILAGTGPRGGFEVDKVTGKTFRYMLKAGLERVDPKRY
                     IFYNHDEAGRLEAEKVLGRMGQRSAAHADKDMNVPGFLTQLKAIKRWGREPQDDMTFI
                     TQPTLIVNGDKDMQVPTENSYTMHEKIKNSQLIIYPNAGHGSIFQNAEEFSKALLAFL
                     EESNA"
     gene            7530..8399
                     /locus_tag="AC1PIW_000921"
     CDS             7530..8399
                     /locus_tag="AC1PIW_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIP6605873.1"
                     /translation="MPKTILITGSTDGIGKHLAMKLASEGHEVILHGRNSEKLRVALS
                     DIQLKTGSKKIHGYLADFSKLADVYRFSQEIKRDFEQIDVLLNNAGAYFGDARVATAE
                     NIEMTFMLSVQVPYILTTELLPLLEKAAGRVIHTSSLMHHFAQTRGLDFGLEQSYSAA
                     MAYNNAKLYTIWLAIAQAEALEKQGSSVTVNAYHPGLIATNLGNDGIKRNLRSRILTS
                     LMKPFSKDLDQGIETGYYLSLSPEVAGVSGRYFAEKRLANVSLKGFDLAKSQALLAYL
                     DKKIQVFKESYGD"
     gene            8392..9567
                     /locus_tag="AC1PIW_000922"
     CDS             8392..9567
                     /locus_tag="AC1PIW_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase family
                     protein"
                     /protein_id="HIP6605874.1"
                     /translation="MTKILLENLQLPNGQVLDNRFFKSAMSETLADSQGAPSDDLIAL
                     YDFWAKQGVGVLVTGNVMVDGRYLGEPGNVVLDSDAHLAQFRKWAAVGKKNGVPIWLQ
                     LNHPGKQMYRSINQTPIAPSAIPISGGSASAFRPPREMTVFDIKEARDKFIAAGLRAK
                     AAGFTGVQLHAAHGYLTNQFLSPADNQRTDRYGGSLDNRMQFLVEVYQGLREKVGPDF
                     TIALKLNASDFKEEGFGFEDCKHVVKIMSDLGIDLIEISGGNYETPVFGSDYENGAGF
                     VTYALALADLTSVSIVSTGGFRKVTQMEEAIKDGVSMIGLARPFVLRPSLVNDYRQVG
                     DLQADGERTGRYILGGEELVLNSKGESVISYADYAIGMIDEIATGDHLKARISLVSE"
     gene            9577..10026
                     /locus_tag="AC1PIW_000923"
     CDS             9577..10026
                     /locus_tag="AC1PIW_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HIP6605875.1"
                     /translation="MQISNKFTIAIHALAFIHLFQDQQRVTSKVLANSIQANPVIIRS
                     VLSGLKEAGIVDAKQGSGGFRLAKTLDDISLYDIFVTVDNIDKKGLFNFHENPHPDCI
                     VGGNIHAAVDDKLVQVQTAMEKELKTMYMSDVLVDLEKAIEEKVKRT"
     gene            complement(10286..10795)
                     /locus_tag="AC1PIW_000924"
     CDS             complement(10286..10795)
                     /locus_tag="AC1PIW_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIP6605876.1"
                     /translation="MERVKRKESLFVESVVEALWELLEDKSFEKISVSELVERAGIGR
                     VTFYRNFSNKEEVLERSFNLESQAWLTERQIDLSDWSDAHLLLALRQFFDFWYEQQDK
                     IRLLTANHLDYLLEEVLDRYFKEKLGDLTDDFHLQFIVGGFFRVLKTWVARGCKESPD
                     DIMRLMSEQ"
     gene            10936..11859
                     /locus_tag="AC1PIW_000925"
     CDS             10936..11859
                     /locus_tag="AC1PIW_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050525 - cutinase activity [Evidence
                     IEA];
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorophyllase/cutinase-like alpha/beta fold
                     protein"
                     /protein_id="HIP6605877.1"
                     /translation="MKKVLIGIGILIACFGIGFLYLASKPSVASNYTEVVETGGAVEK
                     KYLGQGNYDVSYLEINALQNFKKYELYYPTNIETETRKFPVVIFSNGTGVRASKYASV
                     LKHLASWGFIVIGTEEEYSWNGFSSEMSLRLAIKLNDNKTVEGLKSNPFFSKVDLDKI
                     GLSGHSQGGVGVFNAVTEQKHGHMIKTIYAASPANLELSSNLEWDYDPSLISVPTFLV
                     SGTGGGYEDLVVSGKQLTQIYETLPATITKVMARRKEAGHGDMLRLPNGYMVAWFMWR
                     LQGAPEAETAFQGNSPEIVQNKLYQDQRIMN"
     gene            11966..12985
                     /locus_tag="AC1PIW_000926"
     CDS             11966..12985
                     /locus_tag="AC1PIW_000926"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIP6605878.1"
                     /translation="MKKFKKIILWTLGIVASLGIIAYVSVTNHPQLVVGVIQSFMYKE
                     SSPNSYSPLYDPIDGLKENGQYLKSEIAYSKDYPNSFLDITYPDQNLEADRPTLFYFH
                     GGGFFGGSKNMGAPLSASQATYLIDDIVSKGYNVVNVDYALVPDHHFPTPVIQMNQAI
                     AYMKEHAEEYHLNMDNVVLMGSSAGAIMAAQYGSVLANQEYAQLLNIEPSIAKEDVKA
                     LVIDDAPLDYAKFSLGTKILVGNYVSETIYLSEEEINAYNPISHVNKDYPASFLLGSE
                     DRTDMVSLNKALKQAGVENELVDPLAEEGLEKPHGFVANLRTDPVSAKAFERLMIFID
                     DRTKE"
     gene            13207..14490
                     /gene="tig"
                     /locus_tag="AC1PIW_000927"
     CDS             13207..14490
                     /gene="tig"
                     /locus_tag="AC1PIW_000927"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIP6605879.1"
                     /translation="MSVSFEAKETNRGVLTFTIGQDAIKPELDRVFNKVKKDINLPGF
                     RKGHLPRAVFNQKFGEEALYQDVVNALLPAAYEAAVAEAGLEVVAQPKIDVVSMEKGQ
                     DWTITAEVVTKPEVKLGDYKNLAVSVEATKEVTDEEVDAKIEAARNNLAELVIKEGPA
                     AEGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEAQLVGHAAGEEVNVEVT
                     FPEDYQAADLAGKQALFVTKIHEVKAKEVPALDDELAKDIDEEVETLDELKAKYRKEL
                     EASKEVAFDDAVESAALELAVENAEIVELPEEMIHEEVHRAINEFLGGMQQQGISPDM
                     YFQITGTTREDLHKQYEADAEKRTKTNLVVEAVAKAEGFEATEEEINKEIEDLAATYN
                     MEVAQVRSLLSPEMLKHDIAVKKAVEVITSTATVK"
     gene            14541..>15405
                     /locus_tag="AC1PIW_000928"
     CDS             14541..>15405
                     /locus_tag="AC1PIW_000928"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIP6605880.1"
                     /translation="MLQRNSLKNLVRPMQKWENVISVGGVVQVSQGVRITDGEVSHYQ
                     LPWSVLPCLQAIEYDCSFLGSRILLDYLQSNLIISGAFGLFYKEYVIAVGGYDRQTLG
                     EDMELVMKLHYFCRNNNIPYRICYETSAICWSQAPSSIGDLCKQRRRWFLGLYQCLKK
                     YRKMLSRVRFGAVGYFSYAYYMLFEFLAPFIETFGCFVILLSLIYHQLNISFFISLFF
                     LHSAFCSLITFASFLQRVYSQDLFIGPMDLCKAFLATLFRYFFLHWVLNIVRLTSFIG
                     YKKRKREWGEIK"
BASE COUNT         4450 a         3096 c         3598 g         4261 t
ORIGIN      
        1 aattctaaat aatatcattc tgataattca aagggaaata aaaaaaagcg acagaaagcc
       61 gctttgatat tcgtctttta gttctggcaa tcaggacaaa tgccgtagat ggatagcacc
      121 tcttttgtaa ttttgtaacc agtttgggta tgagcttcct tcttcaagct cgggatttct
      181 acatccatga aatcagtgat ttttccacag acttcacaaa taacattgag gtgctcgtgc
      241 cccataaagt cgtaataagt agtattatca ttggtccgtt tcaatttggt cacgaaccct
      301 tcttccaata gaagtttgag gttgttgtaa acagtcgcca gactcatgtt ggggtagtta
      361 ggtattagat cttgataaat catttcagca ctagggtggt catcgctttc gataatgtag
      421 gcaacgactg cttttctagt ttcggtaatg cgtataccct tttcttttag atgttggatg
      481 acgtgttcaa aagcagtttg gttttctgca ttgaagtgag aatggagttc catagtttcc
      541 tcctttcgtt attattttat aaaaattcta agatacaata ttcagtttca ttatagtgta
      601 tttgggaggt tgtgtcaatt ttgagacagg aaatctgtca tagtacagtt tggtgtctca
      661 tgacttgttt acatactttt cctgtgctac aatcaaaaca tgacaagata taaggcaatt
      721 atttcctacg acggacatga cttttctggt tttcagcgcc agccccatgc tcgtacggtt
      781 caggaagaaa tcgaaaaaac gttagtaaga ttgaatagtg gtcagccagt aaccgttcat
      841 ggtgcaggtc gtacagatgc gggggtccac gcttacggtc aggttataca ctttgacttg
      901 gctggcagtc gagatgtgga gaagctccgc tttgccctgg atacccaaac ctctgaggat
      961 attgatgtgg tcagtgtgga gcaggtggcg gatgattttc attgccgcta tgccaagcac
     1021 agcaagacct atgagtttct ggtggatatt gggcgaccta agaatcccat gatgcgccac
     1081 tatgcgacct tctatcccta tgatttggac ttgagcttga tagaagaagc cattcaggac
     1141 ctagtgggaa cccatgattt cacaggcttt acggcttcgg ggacctcggt cgaggacaag
     1201 gtgcggacca ttacagtagc aagtatggaa tatgaccagc agcggcagtt cttgattttc
     1261 accttttcgg gcaacggttt tttgtacaag caggtgcgga atatggtcgg gaccctcttg
     1321 aaaattggca atggtcgcat gcctgttggg cagattaaaa ggattttagc agaaaaggat
     1381 cgtggtttgg cgggtccgac tgcggcaggc aacggcctct atctaaagga gattatctat
     1441 gaagactaag tatattttgg cgctttctgg caacgatatt ttcagcggtg gcgggctcca
     1501 cgcagatctg accacctaca cagtccatgg gctgcatggc tttgtggcgg tgacctgctt
     1561 gacagctatg acggaaaaag gttttgaggt cattccgacg gagactgcgg tctttgccca
     1621 gcagctggct agtttgaagg atgtgccctt ttctgcgatt aagattggct tgcttccctc
     1681 tgtggaaata gcagagcagg ctttggagtt tatcaagact caccaggaga ttcctgtggt
     1741 cttggatcca gtcttggtct gcaaggaaac gcacgatacc gaggtcagtc agttgcgaga
     1801 tgaaatcttg aaattccttc cttatgtcag cattatcacg ccaaatctgg cagaagctca
     1861 gctattgctc caaaaggata tcaaaacggt ggaagagatg aagcaggtgg cagtggtctt
     1921 gtatgacttg ggagccaagg cggtggtcat caagggaggc aatcgtctgg gtggctcaga
     1981 ggcggttgat gttttctacg acggtcagga agtggtggtg ctccagtcgc ctctgctgtc
     2041 agaaaacaat gtgggggcag gctgtacctt tgcgtccagc attgccagtc agcttttgct
     2101 gggtcaagag cctttggagg cggtcaggct gtccaaggac tttgtccatg cggcgattga
     2161 aaaatcagat gattatgggg tggttcaata tgagaaataa acaaacgcaa gaattggtct
     2221 tgctcgctat cttaacagcc ttgagtttgg tattagcctt tgttcatgta ccaactgcaa
     2281 ctggttttgt cacactcttg gatgttgggg tatattttac agccttttac ttgggcaaga
     2341 aagagggagc cattgtcggc ggattgtcag ggcttctgat tgatttcttg ttgggctatc
     2401 cccagtgggc attctttagt ctagtattcc acggggctca gggttatttt gcgggttgga
     2461 caggtaagaa acggattctc ggtttagtct tggcgaccct ctctatggtt gggggctact
     2521 acctggcatc tcgactttat tttaatgacc tgaaagccat tgaaagtgtt ctaagcaata
     2581 ccttgcaaaa cacagttggt ttggtagttg gttacgtctt ggctcaggca atagaaagaa
     2641 ttggtgcagt aaatcatgtt attaagggaa attaaagacc aaactaagca agttgtgcaa
     2701 gaagtcctag aactcagcga tcttcaaaaa ggacagatat ttgtccttgg attgtcctct
     2761 agtgaggtga ttggagggca tatcgggaaa aattccagtc tggaagttgg tgaggtggtt
     2821 gttgaaacca tcttggaaat tctggatcaa aaaggtatct atctagctgt ccaaggctgt
     2881 gaacacctca atcgtgcctt ggtggtggag cgtgaattag caatccagaa ggacttggaa
     2941 atagtcaatg tcttacccac ccttcatgca ggtggttcag gacagctggc ggccttcaag
     3001 tatatgaagg acccggtgga agtcgagttt atcaccgctc aggctggtgt agacatcggc
     3061 gacacagcta tcggcatgca catcaagcac gtccaagttc cgattagacc tagcctgcgt
     3121 gaaattggac aggcccatgt cacagctctc gccagccgac caaaactaat cggcggagtc
     3181 cgagcagcct atcaggaaga tgagatacgg aaaggttcat aatgaaagaa tttacggtta
     3241 aggatttagt gtggacccgt ccaccgagac aattcactat ttcagatgaa gaagttacca
     3301 ttaccaccca cccccagacg gatttgtggc agaagaccta ttaccatttt gtgaatgaca
     3361 atgctcctgt cttacagatg gagacagagg aggactattt ttcttttact gtgaagacgg
     3421 attttgcggg tagtcatact cgatttgacc agtgtggaat tgtcctttat caagattcgg
     3481 agaattggct caaatgttcg atagagtatg aaaatgatga cttccaacac ttgggaagtg
     3541 tggtgactaa tcacggcttt tctgactggg caactactga agtcagttca caaatcaaat
     3601 ccatgtggtt taggctcagt cgcaggggag ctgattactg ttttgaaaat tccgaagatg
     3661 gtatccactt caaacaaatg cgggtttgcc attttcatca aggggcgggg aaaatagctt
     3721 ttgggctata tgcctgttca ccagaaaact ccagttttac agctcgcttt accaatatgg
     3781 aattgagcga ctgcaaatgg caggcccatg atggccaaga gccagatgaa atatagtgag
     3841 agttttaggt aagtagtaac agaaaaatag ttgaaactca tactcttcga aaatcaaaat
     3901 gttccgttgt caacttgcct tgaagaactc ctgttcaatc ttcaccttcg tttcctaggc
     3961 tacttttgat tttcattgag tatcaggaag taactttcga ctagtaagag agttactttt
     4021 tttatctttt gagccaaatc cttgccaaaa aagtctggct tttgataaac ttgtttctgt
     4081 aaatgataca agaacaaaag gagaagacat gacaaaagga taccaacaac gattttcact
     4141 gtccatttta ttatggatgc gtcaggataa agcacgccaa gagggaatgg actactggag
     4201 tggcgggcat gcacagatta ttgcagcttc accgggtcta ttagaatacc gccagcaaca
     4261 tttgagtgag acagaacata gtttttggcc gaaagcgaca ggcttggaaa cggctgttcc
     4321 agaagatcgt cggattgatg gcattgcgga agtgacctat caaaagttat tggcaccaat
     4381 aggcggtcgc cgtcagacgg ccctggcctt tgaagatgag gtcaatgttt tccgtcggac
     4441 tttgatgcac atgggcttcc cttattcatc acgttggtac catactagca ctcaggccca
     4501 aacccaactg cgtgatgtcc tatacttccg tcgcaaagat ggggtgaaga gcggtagctt
     4561 caaaaaattt gttcaagatg gactcgccag tcaactggcg acaatctctg gggtaacgga
     4621 agtacgaaca caggtctatc ttccttggaa taaagcgact tggaataccc caaatgttgc
     4681 ccatgacaat cctaaagaag accatcttca tgcctccatc atcccaggtt ttgcggatca
     4741 ggcggcaaga gaggccttct atgccaacca ggctccgcag ttgaatgcag aagtggtgca
     4801 atactcctca gcagtccacg cttatcatat agaaaaaacc ttgccatttg tcctagacgg
     4861 caaacgcatg taagacaagc gacttcttgg tcgaatgctt tgttaaaaaa atatagtcga
     4921 atgaattagc tttcagacaa ggaaccgagg tgcaggttgc tagcacagcc tagtggctgt
     4981 gctaggttgg agatataact tgcaaagcaa gtcacttctg cagagtacgg caagccgaaa
     5041 gtgacgatgt atcaaagtta attcaaatga ctataaagca aaagacttgc caagaagtct
     5101 agcttatggt aaacttgttc ttgtaaatga tacaagaaca gaaaggaaac cagatggata
     5161 caaagttttc agtagccctt catatactga ccatgatcag tgagagtaag gaaatactta
     5221 gctcgcaagc cttagctgag agcgttggca ccaatgccag ctacatccgc aaggtgattg
     5281 cacttttgaa aaatgcaggc ttaatcactt cgcaccaagg ccgttcaggc taccaattga
     5341 gcaagtctcc taaggatatt agcttattgg aaatctacct tgcgacgcaa gaagtggagc
     5401 atattcgcct atttcagata caccagaatg ccaatctttc ttgtccggtt gggcagcata
     5461 tagaaggcgc aatggttccc atcttttcca gcgttgaaca agaactggaa aatcaactca
     5521 gtcgtcaatc cttagataat gtgattacta atctctatca atccgcacaa gtaacccgaa
     5581 tctgacctcg gtcagatttt ataaactcaa acatagacct gtaagtgata caggaacaaa
     5641 tgatataata gaaaagagaa gaacatgaaa gcagcacaac acacatctta taacaaaaac
     5701 aacatcgccc ttaaccttac agaaattgct aagccacaaa tcaagcctaa ccaagtcctt
     5761 gtcaaagtga ccgcagcagg cgttaacccc ttggacaaca tgatttcacg cggagatgtg
     5821 aaattgattg tcccttacaa attgccccaa acagctggaa atgaagtggt tggcttggtt
     5881 gaagaagtag gttcaagcgt gacgaccttt gcagcagggg atcgtgtctt cggtcgtctt
     5941 ccgcttgata gtatcggtgc ttttgctgag tatgtagcgg ttgacgctca ggctcttgct
     6001 aaggttcctg cctacttgac agatgaagaa gcagctgccg ttcctctgac agctctgacc
     6061 atcatgcagg cacttgacct tatgggggca caatctggca agaccatctt catctcaggc
     6121 ggaactggtg gtgtcggagg tatggccatt ccgattgcca aagccaaggg cttgaccgtt
     6181 atcacaaatg gtgatggggc aagtgcagag cgggtgttgt cattgggtgc agaccgtttt
     6241 atcgactaca agacagaaga ttacaccaaa acacttagcg atgttgacta cgtcttggac
     6301 acacttggtg gtgcagaaac agagaaacaa atgtcaatca tgaaaaaagg tggacagctg
     6361 gtttcccttc gcgccatgcc aaatggcgaa tttgccaaac gtatgaattt gccaaaatgg
     6421 aaacaaatct tgtttggcct agctggtcgt tcatttgaca aaatggcaaa aaaatacggt
     6481 gttcactacc atttcatttt cgtagagagc aatggtcgcc aactgcaaga agtagcagac
     6541 attttcaaca agctcgaaat caaaccgtca atcgatacag tttatccatt tgaagaagtc
     6601 aacgcagcac ttgataaggt tgccaacggt cgttcacgcg gaaaaacagt cctcagtttc
     6661 aactaaaaaa taaaatatac agggaaaaat catgtcatat attacaacca aaaaccaata
     6721 catcaccgtt tcaaacaata aaatcgccta tcgtgagctc agcaagggca agtctgacat
     6781 tcctttggtc atgctggtcc acctagcggc caccttggac aactgggatc ccaaattgct
     6841 ggatttgctg gctgaaaagc accatgtaat tatcctagac cttcctggcg taggagctag
     6901 tcaaggcaag gtggctccga ctattccagg aatggctgag caggctgttg ctattatcaa
     6961 ggctttgggt catgagaaaa tcaatttgct gggtctgtct atgggtggtt ttattgctca
     7021 agaaatcgtt cgtctggata gtcagctggt caaccgattg attttagcag gtacaggtcc
     7081 tcgtggtggt tttgaagttg ataaggtaac tggaaaaacc ttccgctata tgctgaaggc
     7141 tggtttagag cgtgtggatc caaaacgcta cattttctac aatcatgatg aagcaggtcg
     7201 tttggaggca gaaaaagtct tgggtcgtat ggggcaaaga agtgctgcac atgcagacaa
     7261 ggacatgaat gttccaggat ttttgaccca gctgaaagct atcaaacgct ggggaagaga
     7321 gccgcaagat gacatgacct ttattaccca accaactctg atcgtcaacg gtgataagga
     7381 tatgcaagtt ccgacagaaa attcttatac catgcatgaa aaaatcaaaa atagccaact
     7441 gattatttat ccaaatgcag gacacggctc gatcttccaa aatgcagaag agttttcaaa
     7501 agccttacta gcctttttgg aggaaagcaa tgcctaaaac tattttaatc acaggatcga
     7561 cagacgggat tggcaaacac ctagccatga aactagctag tgaaggccat gaagtgattt
     7621 tgcacgggag aaatagcgaa aagctccgtg tagctctcag tgacattcag ctaaaaacag
     7681 gaagtaaaaa aattcatggc taccttgcag acttttccaa gttagcagat gtctatcgtt
     7741 tcagccaaga aattaaacgc gattttgagc agattgatgt cttgttaaac aatgcgggtg
     7801 cttactttgg tgatgcgcgt gtagcaacgg cagaaaatat cgagatgacc tttatgctgt
     7861 ctgtccaagt tccgtatatc ttgacgacgg agttacttcc cttgctggaa aaagcagcag
     7921 gcagggtcat tcatacctct tccttgatgc accattttgc acaaaccaga ggcttggatt
     7981 ttggtttaga gcagagctat tctgcagcca tggcttacaa taatgccaag ctttacacca
     8041 tttggctggc aatagctcag gcagaggcct tagaaaagca aggttcatct gtgaccgtca
     8101 atgcctacca tcctggcttg attgcgacaa atttgggcaa tgatggtatc aaacgcaatc
     8161 tgagaagtcg gattctcacc agtctgatga aaccattttc caaggatttg gaccagggga
     8221 ttgaaacggg ttactactta agcttgtcac cagaggtggc tggagtatca ggtcgttatt
     8281 ttgcggagaa aaggttggct aatgtcagct tgaaaggatt tgatttggca aagagccaag
     8341 ccttactagc ctatcttgat aagaaaattc aagtatttaa ggagagctac ggtgactaag
     8401 attttgttag aaaacctcca actgcccaat ggtcaggtct tagacaatcg ctttttcaaa
     8461 tccgctatga gcgaaaccct ggcagatagt cagggagctc cctcagatga cctgattgcc
     8521 ttatatgatt tttgggccaa gcagggagtg ggtgtcttag tgacaggcaa tgtcatggtg
     8581 gacggccgct accttggtga gccgggcaat gtggtccttg attcagacgc tcatctggct
     8641 caatttcgga aatgggcagc cgttgggaaa aagaacggtg tgcctatctg gctccagctc
     8701 aatcatcctg gcaaacagat gtatcggtcg atcaatcaaa ctccgattgc acccagtgcc
     8761 attccgattt ctggaggttc agcttcggcc tttcgtccac cgagagaaat gactgtgttt
     8821 gacatcaaag aagccagaga taaatttatt gcagcaggcc ttagggcaaa agcagcagga
     8881 tttacaggtg tacagttaca cgctgcacat ggttacttga ccaatcaatt cctctcacca
     8941 gcggataatc agcggacgga ccgctatggt ggcagtctgg acaatcgcat gcaattcttg
     9001 gtggaagttt atcaaggtct gcgtgaaaaa gtgggaccag attttaccat tgccttgaaa
     9061 ttgaatgctt ctgattttaa ggaagagggt tttggttttg aggattgtaa gcatgtggtg
     9121 aaaatcatgt ctgacttggg tattgacctg attgaaattt caggtggcaa ttatgagaca
     9181 cctgtttttg gtagtgacta tgagaatggt gctggatttg tcacctatgc ccttgctcta
     9241 gcagatttga cttctgtttc tattgtttca acaggcggtt tccgaaaagt cactcagatg
     9301 gaggaggcta tcaaggatgg tgtgtccatg attggtcttg ctagaccctt tgtccttcgt
     9361 ccaagtcttg tcaatgatta ccgtcaagtc ggtgatttgc aagcagacgg cgaacggaca
     9421 ggtcgttata ttcttggcgg tgaagagttg gtcttgaaca gcaagggaga aagcgttatt
     9481 tcctatgcag attatgccat tggtatgatt gacgagattg caacaggtga tcatctcaaa
     9541 gcacgtatca gccttgtcag tgagtaaaag attgttatgc agatttcaaa taaatttacc
     9601 attgccattc atgccttggc ttttatccat ctctttcaag accagcaacg tgtcaccagc
     9661 aaggttttgg caaacagtat tcaagccaat cccgttatca ttcgctcggt tctatctggt
     9721 ttgaaggaag cgggaattgt agatgccaag cagggtagcg gcggttttcg tttagcaaag
     9781 acgcttgatg acattagtct ttatgacatc tttgttacgg ttgacaatat tgataagaaa
     9841 ggcttgttca actttcatga aaatccccat cccgactgta tcgtgggggg aaatatccat
     9901 gctgctgtgg acgacaaact cgtccaagtc cagacagcta tggagaaaga attgaaaact
     9961 atgtatatgt ccgatgttct ggttgaccta gaaaaagcca ttgaagaaaa agtgaaaagg
    10021 acttaaatgg gagtgggaaa gaactcgacc attcatagaa gagttcgtct tcccaccccc
    10081 gcacagttga ttaggtcaga tttggagtgt aaaacacgaa caaatctgcc aatcaaccac
    10141 tgcgctgaga tgttgacacg aactctaagt agtggtcctg ggctttttgc ccatcctcac
    10201 tatacacaaa aagacttaga gtgactgaac atcagttgtc taagtctttt tgcttggttt
    10261 ggtcaagaac gttgacaagc ttgctctatt gctcactcat caaccgcatg atgtcatcag
    10321 ggctttcctt acagcctcga gcaacccagg ttttgagcac tcggaaaaat cctcctacga
    10381 taaattgcag gtgaaaatca tcagtcaagt cccccagttt ttctttgaaa tagcgatcca
    10441 agacctcctc gagcaagtag tccaaatggt tggcagtcaa taagcgaatc ttatcctgtt
    10501 gctcatacca aaagtcaaaa aattgccgta aagctagtaa gagatgagca tctgaccaat
    10561 ccgacaagtc aatctgccgt tcggtcaacc aggcttgcga ttccaagttg aaggatcgtt
    10621 cgagcacctc ttccttattg gaaaagtttc ggtaaaaggt cacccgaccg atgccagctc
    10681 gctccaccaa ctctgacaca ctaatctttt caaaagattt gtcttccagt aactcccaca
    10741 gagcctccac gacactctcc acaaataatg actctttccg cttcactctt tccatgcgaa
    10801 acacttcctt tcctttgttt ccttttgggg aaatagcaga gctgggacta gttttcccac
    10861 tgtccatctg ctatactgat aaaaacgaaa cacttgtttc taaaaatata ccataaagga
    10921 cagaaaaaag aaagtatgaa aaaagtttta atcggcattg ggattttaat agcttgtttt
    10981 ggtattggtt tcctgtattt agccagcaag ccatcagtgg cttctaacta cacagaagta
    11041 gttgaaacgg gtggagctgt tgagaaaaag tatcttggac agggcaacta tgacgtttcc
    11101 tatctcgaaa tcaatgcttt acaaaatttc aaaaaatatg aactgtacta tcctacaaat
    11161 attgaaacag aaaccagaaa atttccagta gtgatatttt ctaatggtac cggtgttaga
    11221 gcttctaagt atgcgtctgt tttgaaacat ttggcttctt ggggctttat tgtcattggt
    11281 actgaggaag aatattcttg gaatggattt tcatccgaaa tgagcttgcg acttgctatc
    11341 aagctaaatg ataataagac tgttgaaggc ctaaaatcaa atcctttctt tagtaaagtc
    11401 gatttagata agatcggcct atcggggcat tctcaaggag gagttggggt ctttaatgct
    11461 gttacggaac aaaagcatgg gcatatgatc aaaactattt acgcggcaag tcctgccaat
    11521 ttagaacttt ctagtaattt agaatgggac tatgacccga gcttgatttc tgttccgacc
    11581 tttctagtat cgggtacggg aggtggatat gaggatttgg tagtgtctgg caagcagttg
    11641 acacagattt atgagacatt accagctacc atcacgaagg taatggctcg gagaaaagaa
    11701 gcaggacatg gtgatatgct tagacttcca aacggctata tggtagcttg gtttatgtgg
    11761 cggttacaag gagcccctga agcagagacg gcatttcaag gaaactcccc agaaattgtt
    11821 caaaacaaac tctatcaaga tcaaagaata atgaactagg atttgtatca ttgacggtca
    11881 aaagcgagtt ctgtctttct attgtttgtt cttgttttat gttagtagct tattcaaaaa
    11941 atgtgtaaaa agagtgtaat tcttcatgaa aaaattcaaa aaaatcatct tatggaccct
    12001 tgggattgta gccagtctcg gtatcattgc ttatgtgtct gttaccaatc atccgcagtt
    12061 ggtagtcggt gtcattcaat cgtttatgta caaagagagt tcaccgaact cttacagtcc
    12121 tctttatgat cccattgatg gtctgaaaga aaatggtcag tatttgaaaa gtgagattgc
    12181 ctatagtaag gactatccca acagtttttt agatattacc tatccagacc aaaatcttga
    12241 agctgaccga ccaaccctct tctatttcca cggtggtgga ttctttggag gtagcaaaaa
    12301 tatgggggcc cctctgtcag ctagccaagc gacctacctc attgatgaca tcgtgtcaaa
    12361 aggatataat gttgtcaatg tggattatgc tcttgtacca gaccaccatt tcccaacgcc
    12421 agtgattcaa atgaatcagg ctatcgccta catgaaggaa cacgctgaag aatatcactt
    12481 gaacatggac aatgtcgtct tgatgggttc atcagcaggt gccatcatgg cggcacaata
    12541 tggtagcgtc ttagccaatc aagagtacgc tcagctattg aatattgaac caagtattgc
    12601 aaaagaagat gtcaaggctc tggttataga cgatgctcct ttagattatg ccaaatttag
    12661 cctaggtacg aagatcttgg ttggaaatta tgtttcagaa accatctatc taagtgagga
    12721 agaaatcaat gcctataacc ctatttccca tgtgaataaa gactacccag ctagtttcct
    12781 tctgggaagc gaggatcgaa cagatatggt gtcgctcaac aaggctttga agcaagcggg
    12841 agttgaaaat gaattggtcg atcctttggc agaagaaggg cttgaaaagc ctcatggttt
    12901 tgtggcgaat ttacgaacag accctgtgtc tgccaaagca tttgaacggt tgatgatttt
    12961 tatagacgat agaaccaagg aatagtcgga gtgttgataa gttggaaact gatagggatt
    13021 tgtccaatca atctgtcgct ctgacaacca tgtttgtact cagaaagtct acgccgaaaa
    13081 attccaagtg agaacaataa gtcccagctc tgccatttcc caaaagtacg ttataaaatc
    13141 gttgaaatcc ttgtcaattg tgttataatg aagagtatgc aataaaattt taaggagaat
    13201 gacagaatgt ctgtatcatt tgaagcaaaa gaaacaaatc gcggcgtttt gactttcaca
    13261 atcggtcaag atgctatcaa accagaattg gatcgcgttt tcaacaaagt taagaaagat
    13321 atcaatcttc caggtttccg taaaggtcac ttgccacgtg ccgttttcaa ccaaaaattt
    13381 ggtgaagaag cactttatca agatgttgta aatgcattgt taccagcagc ttatgaagca
    13441 gcggttgcag aagctggtct tgaagtcgtt gcacaaccaa aaatcgacgt tgtatctatg
    13501 gaaaaaggtc aagactggac aatcactgcg gaagttgtga caaaacctga agtaaaattg
    13561 ggtgactaca aaaacttggc agtttcagta gaagctacta aagaagtaac agacgaagaa
    13621 gttgacgcaa aaatcgaagc tgcacgcaac aacttggctg aattggttat caaagaaggt
    13681 ccagcagctg aaggcgatac agttgttatc gactttgtag gttcaatcga cggtgttgaa
    13741 tttgacggcg gtaaaggtga gaacttctca cttggacttg gttcaggtca attcatccca
    13801 ggttttgaag cgcaattggt tggtcacgca gctggtgaag aagtaaacgt tgaagtgact
    13861 ttcccagaag actaccaagc agctgacctt gctggtaaac aagccctctt cgtaacaaaa
    13921 atccatgaag taaaagcaaa agaagttcca gctttggatg acgaattggc aaaagacatc
    13981 gacgaagaag tggaaacact tgatgagttg aaagctaaat accgcaaaga attggaagca
    14041 tcaaaagaag ttgcctttga cgatgcagtt gaatcagcag ctcttgaatt ggctgtagaa
    14101 aacgctgaaa tcgttgaatt gccagaagaa atgattcatg aagaagttca ccgtgcaatc
    14161 aatgaattct tgggtggtat gcaacaacaa ggtatctcac cagatatgta cttccaaatc
    14221 actggtacaa ctcgtgaaga ccttcataaa caatacgaag cagatgctga aaaacgtact
    14281 aagacaaact tggttgttga agcagtagct aaagcagaag gtttcgaagc aactgaagaa
    14341 gaaatcaaca aagaaatcga agacttggca gcaacttaca atatggaagt ggcacaagtt
    14401 cgtagcttgc tttcaccaga aatgcttaaa cacgacatcg ctgtgaagaa agctgtagaa
    14461 gtgattacaa gcactgcaac tgtaaaataa ttatttggaa aactcgactc tggtcgggtt
    14521 ttttgtttaa tcatctttcg atgttacaaa gaaactctct gaaaaacctt gtccgtccca
    14581 tgcagaaatg ggagaatgtc atatctgttg gtggagtcgt ccaagtttca caaggggtac
    14641 gaataactga cggagaggtc agccattacc agttgccgtg gtcggtgctc ccatgtttac
    14701 aagctatcga atatgactgt tcttttttag ggagtagaat tttattggac tatttgcaat
    14761 ccaatctcat catatcaggt gcctttgggt tattttacaa ggagtatgtc atagcagttg
    14821 ggggttatga taggcagaca ctgggagaag atatggagct agtcatgaaa ttgcattatt
    14881 tctgtagaaa taacaatatt ccttatcgaa tctgctatga aaccagcgcc atttgttgga
    14941 gtcaagcacc gagctctatc ggtgatttat gcaaacaacg ccgtcgctgg ttcttgggac
    15001 tctatcaatg tctaaaaaaa taccgaaaaa tgttatcgcg agttcgtttt ggggcggtgg
    15061 gatatttttc ctatgcttac tatatgctat ttgaattttt ggctccgttt atagagactt
    15121 tcggttgttt cgtgattcta ctttcgttga tctatcatca actaaacatc tcatttttca
    15181 tatcgctctt tttcctccat agtgcttttt gctcactaat cacctttgcg tcatttttgc
    15241 aacgcgtcta ctcgcaagat ttatttattg gacctatgga tttgtgtaaa gccttcctgg
    15301 caaccttgtt tagatatttt ttcttgcatt gggtattgaa tattgtccgt ctgacaagtt
    15361 ttattggata taaaaagaga aagcgtgaat ggggagaaat caaac
//
LOCUS       DBNFCK010000037        15287 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.037, whole genome shotgun sequence.
ACCESSION   DBNFCK010000037 DBNFCK010000000
VERSION     DBNFCK010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 15287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15287
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.037"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            405..692
                     /locus_tag="AC1PIW_000929"
                     /pseudo
     CDS             405..692
                     /locus_tag="AC1PIW_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1216..1854)
                     /locus_tag="AC1PIW_000930"
     CDS             complement(1216..1854)
                     /locus_tag="AC1PIW_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIP6605881.1"
                     /translation="MIIHKSVNPVAFQNTYYLENDTHLIIIDPGSDWTKIQVTIEKIG
                     KPISAILLTHTHYDHIMSLDILRETYNFPPVYVAENEASWLYTPEMNLSGLPRHDDME
                     NVVCRPAEKIFQYQEDYQFDGFHFKVVPTPGHSIGGVSFIFPEDECVITGDALFRETV
                     GRTDLPTSNFDDLITGIREHIFTLPNHYTVHPGHGQNTSVGHEKNFNPFFRM"
     gene            1962..4430
                     /locus_tag="AC1PIW_000931"
     CDS             1962..4430
                     /locus_tag="AC1PIW_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HIP6605882.1"
                     /translation="MNSMKDTRTSNRYAVVDLEATGTGTDAKIIQIGIVLVENGEIID
                     SYATDINPYELLDDHIKNLTGITDQQLSQAPDFGQVASTIYDMIGDAIFVAHNVKFDA
                     NLLAEALFFEGYELLTPRVDTVELSQLFFPTFEKYSLGNLAEHLELGLDQAHTAISDA
                     MATARLLIKIQEKIKSLPRSIVEKILDLADNLLFESRLVIDEMVPVLSENLSYDLESI
                     HDLVLKKPEEIKSAYRLSEDFSTNIALLGLHERKKQTAFAQVVEKRLVDEEKVHFIQA
                     QAGLGKTYGYLLPLLAKSDQPLLVTVPTKLLQEQIMEKEGSKLKEIFRIPIVSLKSPK
                     HFIKLDNYWKTLQRQDDNRLVNQFKMQVLVWLCETTTGDLDELKQKQRYQAYFDEIAH
                     DGKLEPESLFWGLDFWQRLNQQALSSRLLITNHAYFLSHLKDQDPHMDKRMVVIDEAQ
                     KFLLAAENLATASQDLTSMLQVLQSKKDRATAILDQRLYESCQFELNHFLSRFRQHGQ
                     REVQKAELHQLGQNLEELGDVDLNDLHQLIDYYDTFWLEEKNLEEKRLAYLRSSKDSL
                     LNATNYLPDTKIFCISATLTISKKVSLADLLGFEQVTLDLIPTQTVTNQRLLFPTHLP
                     DILAWTKEEHAAYLATTLGEIAELGRPILVLFTSISMLLQVSDLLEDNNIPHLAQHKH
                     GQEMTLKRKFERGECQILLGTGVFWEGVDFASQNQLIQVITRLPFDNPKDRFVQKINH
                     HLREEGKNPFYDYSLPMMMIKLKQAIGRTNRHDKQDSLVLVLDPRVHTKRYGQQILSF
                     FEKDYLMLSVDAKEIEGAIQDFFE"
     gene            4590..5666
                     /gene="proB"
                     /locus_tag="AC1PIW_000932"
     CDS             4590..5666
                     /gene="proB"
                     /locus_tag="AC1PIW_000932"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HIP6605883.1"
                     /translation="MTEKTIVFKVGTSSLTQENGSLDRIKIARITNQLAQLHQKGYQI
                     VLVTSGSIAAGFRRLGFDKRPTKIAEKQASAAVGQGLLIEEYTQNLMKDGIVSAQILL
                     TQDDFADARRYQNASQALQVLLKQRAIPIINENDTIAIEEIKVGDNDTLSAQVASLLK
                     ADLLVLLTDVDGLYTANPNSDPTAQHLPQIKEITEDLFAMAAGAGSSNGTGGMTTKLQ
                     AAQIATKSGVPVFICSSKEDTALLQAVTQANRGTLFLADDHAMNQRKQWMAFYARTDA
                     AVEVDAGAVDAMLHQGRSLLATGVKALEGDFEVGQVVEVYSQADHRLIGKGRVKLSSK
                     DLQDQLANGRAEGVLIHRNDWVSL"
     gene            5731..6969
                     /locus_tag="AC1PIW_000933"
     CDS             5731..6969
                     /locus_tag="AC1PIW_000933"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HIP6605884.1"
                     /translation="MTTTQVLLDSLLANKASINLATTEQKNQALSAMADQLVAQTEAI
                     LAGNAIDMEHAQGKISQVMQDRLLLTEERIEAMADGIRALIGLPDPVGLVLEESTRAD
                     GLNICKKSIPFGLVGMIYESRPNVTSDAAALAIKSGNAVILRGGKEAFHSAKAIVTAL
                     KSGLEEAGVSPKVIELVQDTSRVSATELMTAKGKIDLLVPRGGAGLIQAVVENATVPV
                     IETGTGICHVYVDKDADLDKALRIVVNAKTSRPSVCNAAEVLLVHEEIASQFLPRLEE
                     ALSGQVELRADSQAQALLNQARPAGDQDFDTEFLDYIMAVKVVSSVEEAISHIAQHST
                     GHSEAIVTENSQTAEHFTLHVDSAAVYVNASTRFTDGGEFGLGCELGISTQKMHARGP
                     MGLREMTTYKYIITGDGHIR"
     gene            6979..7764
                     /gene="proC"
                     /locus_tag="AC1PIW_000934"
     CDS             6979..7764
                     /gene="proC"
                     /locus_tag="AC1PIW_000934"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HIP6605885.1"
                     /translation="MKIGFIGLGNMGAALAQAVSQQPDTQLLLSNHNPAKAQALQEKV
                     GGQLFSNGQIAEQAEIIFLGVKPHLIQTVLSGLQDQISQNPSAIWISMAAGVPLDSLA
                     AFVSADKVIRIMPNTPVAIGQGMTTYSLVNQNLAPLLEQILEKSGKVQQVPESLIDAA
                     TAIAGCGPAFVYQMIEALTDAGVQNGLTAQDAKVLAAQTLAGTAQMVLTSDKHPAQLR
                     QEVTSPGGSTIAGVVALEKEGFRYAIIKAVAAALKKTRKLGKK"
     gene            complement(7787..8191)
                     /locus_tag="AC1PIW_000935"
     CDS             complement(7787..8191)
                     /locus_tag="AC1PIW_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIP6605886.1"
                     /translation="MAGRKISPQSLKNLYQSNKEANQLTKESIETALLFLLEKKELKQ
                     ISVSELVRKAGVSRNAFYRNYKSKEEILEDYYERTSSNLKKKWHDLQDKVQKDGVKQS
                     FADFVQEQKRKAEQSKALSNVSQWIKEKTKRD"
     gene            8318..9169
                     /locus_tag="AC1PIW_000936"
     CDS             8318..9169
                     /locus_tag="AC1PIW_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIP6605887.1"
                     /translation="MTKWKIVADSGCDYRQLANLAPDTEFISVPLTIQVGEQAFVDDA
                     SLDIDHMMEVMEASKSAAGSACPSPQAYQAAFEGAENIIVVTITGGLSGSFNAARVAR
                     DMYIEEHPNVNIHLIDSLSASGEMDLLVDEINRLISAGLDFPQVVEAITHYREHSKLL
                     FVLAKVDNLVKNGRLSKLVGTVVGLLNIRMVGEASAEGKLELLQKARGHKKSVTAAFE
                     EMKKAGYDGGRIVMAHRNNAKFFQQFSELVKASFPTAVIDEVATSGLCSFYAEEGGLL
                     MGYEVKA"
     gene            complement(9268..10527)
                     /locus_tag="AC1PIW_000937"
     CDS             complement(9268..10527)
                     /locus_tag="AC1PIW_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HIP6605888.1"
                     /translation="MKKYQVIIQDILTGIEEHRFKRGEKLPSIRQLREQYHCSKDTVQ
                     KAMLELKYQNKIYAVEKSGYYILEDRDFQDHTVELNPTDFQELPYEDFRICLKESLIG
                     RENYLFNYYHQQEGLAELISSVQSLLMDYHVYTKKDQLVITAGSQQALYILTQMETLA
                     GKTEILIENPTYSRMIELIRHQGIPYQTIERNLDGIDLEELESIFQTGKIKFFYTIPR
                     LHNPLGSTYDIATKTAIVKLAKQYDVYIIEDDYLADFDSSHSLPLHYLDTDNRVIYIK
                     SFTPTLFPALRIGAISLPNQLRDIFIKHKSLIDYDTNLIMQKALSLYIDNGMFARNTQ
                     HLHHIYHAQWNKIKDCLEKYALNIPYRISKGSVTFQLSKGILSPSIQHMFGKCYYFSG
                     QKADFLQIFFEQDFADKLEQFVRYLNE"
     gene            10650..11384
                     /gene="yaaA"
                     /locus_tag="AC1PIW_000938"
     CDS             10650..11384
                     /gene="yaaA"
                     /locus_tag="AC1PIW_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HIP6605889.1"
                     /translation="MKIIIPNAKEVNTNLENASFYLLSDRSKPVLDAISQFDVKKMAA
                     FYKLNEAKAELEADRWYRIRTGQAKTYPAWQLYDGLMYRYMDRRGIDSKEENYLRDHV
                     RVATALYGLIHPFEFISPHRLDFQGSLKIGNQSLKQYWRPYYDQEVGDDELILSLASS
                     EFEQVFSPQIQKRLVKILFMEEKAGQLKVHSTISKKGRGRLLSWLAKNNIQELSDIQD
                     FKVDGFEYCTSESTANQLTFIRSIKM"
     gene            11489..12934
                     /locus_tag="AC1PIW_000939"
     CDS             11489..12934
                     /locus_tag="AC1PIW_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIP6605890.1"
                     /translation="MKKRSGRSKSSKFKLVNFALLGLYSITLCLFLVTMYRYNILDFR
                     YLNYIVTLLLVGVAVLAGLLMWRKKARIFTALLLVFSLVITSVGIYGMQEVVKFSTRL
                     NSNSTFSEYEMSILVPANSDITDVRQLTSILAPAEYDQDNITALLDDISKMESTQLAT
                     SPATSYLTAYQSMLNGESQAMVFNGVFTNILENEDPGFSSKVKKIYSFKVTQTVETAT
                     KQVSGDSFNIYISGIDAYGPISTVSRSDVNIIMTVNRATHKILLTTTPRDSYVAIADG
                     GQNQYDKLTHAGIYGVNASVHTLENLYGIDISNYVRLNFISFLQLIDLVGGIDVYNDQ
                     EFTSLHGNYHFPVGQVHLNSDQALGFVRERYSLTGGDNDRGKNQEKVIAALIKKMSTP
                     ENLKNYQAILSGLEGSIQTDLSLETIMSLVNTQLESGTQFTVESQALTGTGRSDLSSY
                     AMPGSQLYMMEINQDSLEQSKAAIQSVLVEK"
     gene            12952..13641
                     /locus_tag="AC1PIW_000940"
     CDS             12952..13641
                     /locus_tag="AC1PIW_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HIP6605891.1"
                     /translation="MNNQEVNAIEIDVLFLLKTIWRKKFLILLTAVLTAGLAFVYSSF
                     LVTPQYDSTTRIYVVSQNVEAGAGLTNQELQAGTYLAKDYREIILSQDVLTQVATELN
                     LKESLKEKISVSIPVDTRIVSISVRDADPNEAARIANSLRTFAVQKVVEVTKVSDVTT
                     LEEAVPAEEPTTPNTKRNILLGLLAGGILATGLVLVMEVLDDRVKRPQDIEEVMGLTL
                     LGIVPDSKKLK"
     gene            13651..14328
                     /locus_tag="AC1PIW_000941"
     CDS             13651..14328
                     /locus_tag="AC1PIW_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HIP6605892.1"
                     /translation="MAMLEIARTKREGVNKTEEYFNAIRTNIQLSGADIKVVGITSVK
                     SNEGKSTTAASLAIAYARSGYKTVLVDADIRNSVMPGFFKPITKITGLTDYLAGTTDL
                     SQGLCDTDIPNLTVIESGKVSPNPTALLQSKNFENLLATLRRYYDYVIVDCPPLGLVI
                     DAAIIAQKCDAMVAVVEAGNVKCSSLKKVKEQLEQTGTPFLGVILNKYDIATEKYSEY
                     GNYGKKA"
     gene            14367..15098
                     /gene="cps4B"
                     /locus_tag="AC1PIW_000942"
     CDS             14367..15098
                     /gene="cps4B"
                     /locus_tag="AC1PIW_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4B"
                     /protein_id="HIP6605893.1"
                     /translation="MIDIHSHIIFGVDDGPKTIEESLSLISEAYRQGVRYIVATSHRR
                     KGMFETPEKIIMINFLQLKEAVAEVYPEIRLCYGAELYYSKDILSKLEKKKVPTLNGS
                     CYILLEFSTDTPWKEIQEAVNEMTLLGLTPVLAHIERYDALAFQSERVEKLIDKGCYT
                     QVNSNHVLKPALIGERAKEFKKRTRYFLEQDLVHCVASDMHNLYSRPPFMREAYQLVK
                     KEYGEDRAKALFKKNPLLILKNQVQ"
BASE COUNT         4557 a         2901 c         3472 g         4357 t
ORIGIN      
        1 atgggctgcc tttttgtctt ttgacagcga aggccgcttg acataagcat ttcttccaat
       61 aggaattccc gtgtcgaatc ttggtacttc gtttttccca ctccctagga ttttttatta
      121 tatgactttt agtccatcac aatccgtaaa gtatgtgaca aatgaaccga ggatgggcaa
      181 aaaatggtcc tataatttct gtagcgggta aaaccactat ggagcctttt aaatgtaaaa
      241 gtcatatgac ctataatgaa aagcgaaaaa acttacatta gaaagaggtc atatggaata
      301 aatcaagagt taggggagtg actcttgatt cctgagccta gaaatagaaa agcgaagatc
      361 ttatcacaaa ttttcttgga atagaagaca aaaatatcgc tatcatgact gaatatgatg
      421 tggaaactca cttagattgt gtgtctccaa aatgtcttgc ctgaaaggga caaatagtga
      481 aatacaactt ccaaagagca tctaaaatcc tttgcttaga agcagctgat aatcctctct
      541 ccttatccat cttagaaagc gtcggttcaa gtgtaaagga tgcgggaaaa tggttgtcgc
      601 tgaaattcac ttattaaaaa aacgaccaaa tctctgttgc tattaaccag aaaacccccc
      661 aattactact cgttgagaat caagcaatga gtaagcaatg ttcacctgat actattgtca
      721 tcaaggatag cttttctatc ctattcggaa ctataaagta agagtttttg aagggggtag
      781 atattggttg catgtattga aaaacttgtg gttccatggt ttttaaagtt actctatctg
      841 gctatatttt gaactatgtt attacaccag tttctgatta tgtagtgaat tgcttgttgg
      901 aaggctatca gtaatctgtg attttagcag atttagtcag gagtttaaag acaacttgga
      961 acagaaggat agaacatgga aggagttaat tagtataaca aatggagatt attagccatg
     1021 aggcgaacca ttgaaactcg ttttctgaat catacgcttt gtttgatata gaacaaacct
     1081 ttgccagagg actgctcgga ttacagctac ggattgaaca aatcctactt atctacaatc
     1141 taagctattt tgactttaac tagtagcacg ggtaaaaaaa ctgcctagtt caatcagaac
     1201 tgggcagtac ttattttaca ttctaaaaaa tggattgaaa tttttctcat gtccaacgct
     1261 ggtattttga ccatgacctg gatggacagt atagtgattt ggcagggtaa agatatgttc
     1321 tctgatgcca gtaatcaggt catcaaagtt gctggttggc aggtctgtgc gtcccacagt
     1381 ttcacggaaa agtgcgtctc cagtgatgac acattcatcc tctgggaaga taaaggaaac
     1441 accaccaata gagtgaccag gtgttggaac taccttgaaa tgaaaaccgt caaactggta
     1501 gtcttcttgg tattggaaga ttttttcagc aggtcggcag actacatttt ccatatcatc
     1561 atgacgaggt agacctgata gattcatctc tggtgtgtag agccagctgg cttcattttc
     1621 agccacatag acaggcggga aattgtaggt ctcacgcagg atgtccaaac tcataatatg
     1681 gtcatagtga gtgtgagtca ggagaatagc cgaaattggt tttccgattt tttcgatagt
     1741 tacttgaatt tttgtccagt cactcccagg gtcaatgata atcagatggg tatcattttc
     1801 aaggtagtag gtattttgaa aagcgacagg attgacagat ttgtgaataa tcattctttt
     1861 tccttcttct aacagttact gatgttacta gtctaacaaa aaaacacgcc ttcttcatgg
     1921 aatttgtttg gagctttttc catgagaggg aatatgctat aatgaacagt atgaaagaca
     1981 caagaacaag caatcggtat gccgttgtag atttggaagc aactggtaca ggtaccgatg
     2041 caaaaatcat tcaaatcggg attgttctcg ttgaaaatgg agaaatcatt gacagttatg
     2101 caacggatat caatccctat gagctactgg atgaccatat caagaatctg acaggtatca
     2161 cagaccaaca gctaagtcag gctccagatt ttggtcaggt agcaagtacc atttacgaca
     2221 tgattggaga tgctattttt gtagcccata atgtcaaatt tgatgctaat ctcttggcag
     2281 aagcactctt ttttgaaggt tatgaactgt tgacaccgcg tgtagatacg gtagaattat
     2341 cacagctatt tttcccgact tttgaaaaat atagcttggg aaaccttgca gagcatttgg
     2401 aacttggttt ggaccaggca cacacggcca tatcagatgc catggctacg gcgcgcttgc
     2461 tcatcaaaat tcaagagaaa ataaagagct tacccaggtc tattgttgaa aagattcttg
     2521 atttggcgga caatctgctc tttgaatccc gtttggtcat tgatgagatg gtgcctgttc
     2581 tgtcagaaaa tctatcatac gatttagaat ctattcatga cttggtcttg aaaaagccag
     2641 aagaaatcaa gtcagcctac cgattatctg aagatttttc gaccaatatt gcccttctcg
     2701 gcttgcatga acgaaaaaaa caaactgctt ttgctcaagt agtagaaaaa cgcttggttg
     2761 acgaagaaaa agtgcatttt attcaagcgc aggcaggttt ggggaagact tacggttatc
     2821 tcttgccttt acttgctaag tcagaccagc ctttattggt caccgtaccg accaagctcc
     2881 ttcaagagca gattatggaa aaggaaggga gcaagcttaa agagatattt cgtattccca
     2941 tagtctctct aaaatcaccc aagcatttta tcaagttaga caactactgg aaaaccctgc
     3001 agaggcagga tgataatcgc ctggtcaatc aattcaaaat gcaggtgctt gtctggttat
     3061 gcgaaaccac gacaggtgat ttggatgagc ttaagcaaaa acaacgctat caagcttact
     3121 ttgatgagat tgcccacgat gggaaattgg agcctgaaag tcttttttgg ggcttggatt
     3181 tttggcaacg attgaaccaa caagctctct ctagtagact gctcattacc aaccacgctt
     3241 attttctcag ccatcttaag gaccaagatc ctcacatgga taagcgtatg gtggtgattg
     3301 atgaagcaca aaagtttctc ttggctgctg aaaatctagc gacggcttcg caagatttga
     3361 caagtatgtt acaagtattg caaagtaaaa aggatagagc gacggctatt ttggaccaac
     3421 gtttgtatga gtcttgccag tttgaactca accacttttt gagtcgtttt cgtcagcatg
     3481 gtcaacgaga ggttcaaaag gctgaacttc accaacttgg tcagaacctg gaggagcttg
     3541 gagatgttga tttgaacgac ttgcatcagc tcatagacta ttacgatacc ttttggttgg
     3601 aggagaaaaa tttagaagaa aagcggcttg cctatttgcg gagctccaag gatagcctcc
     3661 taaatgcgac caactatctg ccagatacta agattttctg catcagcgcc accttgacca
     3721 tcagcaaaaa ggtcagtctg gcggatctgc tgggctttga gcaggtcacc ttggatttga
     3781 taccgactca aactgtgacc aaccagcggc tactttttcc gacccatctc cccgacatcc
     3841 tcgcttggac caaggaggag catgcagcat atttggctac tactttagga gagatagcag
     3901 agctgggacg accgattttg gtcttgttta catcgatttc tatgctcttg caggtgtcgg
     3961 atttgttaga agacaataac attccccacc tagcccagca caagcatgga caggaaatga
     4021 ctctcaaacg caaatttgaa cgaggggaat gccaaatttt actcggcaca ggtgtattct
     4081 gggagggcgt tgactttgcc agtcagaatc aattgattca ggttattaca aggctcccat
     4141 ttgacaatcc aaaggaccgg tttgtacaaa aaatcaatca ccatctgcgt gaagaaggga
     4201 aaaatccatt ttatgattac agcctgccta tgatgatgat taagctcaaa caagccatcg
     4261 gtcgtaccaa tcgccatgac aaacaagatt cactagtctt ggttttggac ccgcgtgttc
     4321 acaccaaacg ctatggacag caaattctgt ccttctttga gaaagattat ttaatgttaa
     4381 gtgtggatgc gaaagaaata gaaggagcta ttcaagattt ttttgaataa gttgaaaggg
     4441 agccacaagg cttccttttg tttccagcgt tttttaatgc actcatgatt aggaatctat
     4501 tccctctctc cttgccgaat aacagtgatt ttctttatga ttaaaatatg gtataatttt
     4561 ccaaatatta gtttttgtga gaagagatta tgacagaaaa aacaattgta tttaaggtcg
     4621 ggacaagttc tctgacgcaa gaaaatggca gtctggaccg tatcaagatc gctcggatta
     4681 ccaatcagct ggctcagttg caccagaaag gttaccagat tgtgctggtg acgtcgggct
     4741 cgattgctgc tggttttcgg agattgggat ttgacaagcg accgaccaag attgcggaga
     4801 agcaggcttc tgcggccgtt ggacaaggtt tgctgattga ggaatatacg caaaatctaa
     4861 tgaaggacgg catcgtatcg gctcagattt tactgaccca ggatgatttt gcggatgcca
     4921 gacgttatca gaatgcctct caagccttgc aggtcttgct caagcaacgg gctattccta
     4981 ttataaatga aaatgatacc attgccattg aggagattaa ggtgggggat aacgatactt
     5041 tatctgctca ggtggcttct ctcttgaaag cagacctatt ggtgctcttg acggatgtgg
     5101 atggacttta cactgccaat cctaacagcg atccgactgc ccagcacttg cctcaaatta
     5161 aggagattac ggaagattta tttgccatgg cagcaggagc aggctcgtcc aatggaacag
     5221 gtggcatgac caccaaattg caggcggccc agattgcgac caagtcaggc gtgcccgtct
     5281 ttatctgctc ttcaaaagag gataccgccc tcttgcaggc ggtcacccaa gccaatcgtg
     5341 gaacgctctt tttagcagat gaccatgcca tgaaccaacg caagcagtgg atggccttct
     5401 atgctcggac agacgcggcg gttgaagtgg atgcaggagc ggttgatgct atgttgcacc
     5461 agggccgcag tttgttagct actggtgtca aagccctcga aggagacttt gaagtgggcc
     5521 aggtcgtaga agtttacagc caagcagacc atcgtttgat tggtaaaggt cgggtcaaac
     5581 tgtcctccaa ggacttgcag gaccagttgg caaatggcag agcagagggc gtgttgattc
     5641 accgcaacga ttgggtgagc ttatagttgt tcagtttgtc tcgattatgt tgacgaacga
     5701 gtaaactgaa agactaaata ggagaaattt atgacaacaa cacaagtatt attagatagt
     5761 ttattggcca acaaggcttc tatcaaccta gcgacaacag aacagaaaaa ccaggctcta
     5821 tcagcaatgg cagaccagtt ggttgctcag actgaggcaa ttttagcagg caatgccatc
     5881 gacatggaac atgcccaagg caagattagc caggtgatgc aggaccgttt gctgctgaca
     5941 gaggaacgga ttgaagctat ggcagatggc attcgtgcct tgattggctt gccagatcct
     6001 gtcggattag tcttggaaga atccactcgg gcagacggct tgaacatttg caagaaatcc
     6061 attccctttg gtttggtggg aatgatttac gaaagccgac caaacgtgac ttcagatgcc
     6121 gctgccttgg ccatcaagag tggtaatgcg gtcatcttgc gtggtggcaa ggaagctttt
     6181 cattcggcca aagcgattgt cacagcactc aagagtggct tggaagaagc tggtgtctca
     6241 ccaaaggtca tcgagttagt ccaagatacc agccgggtct ctgcgacgga gttgatgacc
     6301 gcaaaaggca agattgattt attggtgcca cgtggtggag caggactcat tcaagccgta
     6361 gtagaaaatg cgactgtgcc agttattgaa acaggaacag gtatctgcca tgtctatgtg
     6421 gacaaggatg cggacttgga caaggccttg cggattgtgg tcaatgccaa aaccagtcgt
     6481 ccctcagtct gcaatgcggc ggaagtcttg ttggtccatg aagaaattgc cagccaattc
     6541 ctacctcgct tggaagaagc tctttctggt caggtggaat tacgtgccga cagtcaggcc
     6601 caggctctcc tcaaccaagc cagaccagca ggcgaccaag attttgacac agaatttttg
     6661 gactatatca tggcggtcaa ggtcgtttca agtgtagaag aggctatcag ccatatcgcc
     6721 caacattcga ctggacatag cgaggcgatt gtgacggaaa acagccagac agcagagcat
     6781 tttacactcc atgtggactc ggcagcagtc tatgtcaatg cctcgacccg ctttacagac
     6841 ggtggagagt tcggtctggg ctgtgagctg ggtatttcca cccagaagat gcacgcccgt
     6901 ggtccaatgg gactgcgtga aatgaccact tacaagtaca tcatcacagg cgacggccac
     6961 attcgttagg aggacaagat gaagattgga tttattggac tgggcaatat gggagcggcc
     7021 ctagcccaag cggtcagcca gcagccagat acccagctcc tcctcagcaa ccataaccca
     7081 gcaaaagccc aggctcttca agagaaggta ggcggtcagc ttttttctaa tggacaaata
     7141 gcagagcagg ccgagattat tttccttggg gtcaagcctc acctgattca gacagtcttg
     7201 tcaggcttgc aggaccagat tagccagaat ccgtcagcta tctggatttc catggcagca
     7261 ggagtgcccc ttgacagcct ggcagcattt gtatcggcag ataaagttat ccgtatcatg
     7321 cccaatacac cagtcgctat cggccaaggc atgacaacct atagcctggt caatcaaaat
     7381 ctcgctccgc tattggaaca aatcctagaa aaatcaggca aggtccagca agtgccagag
     7441 agcctaatcg acgcagcaac ggctatcgca ggctgcggac cagcctttgt ctatcagatg
     7501 attgaggctc tgacagatgc aggggtgcaa aacgggctga cggcccagga tgccaaggta
     7561 ttggcggctc agaccctggc aggaactgct cagatggtct taaccagcga taaacaccca
     7621 gcccagctga gacaggaagt gacctcgcca ggcggctcga ctatcgcagg tgtggtagct
     7681 cttgaaaaag aaggcttccg ttacgccatt atcaaggcag tcgccgccgc cctcaaaaag
     7741 actagaaaat taggcaaaaa atagaagaca gcaaaaaacg ggacttttaa tcccgttttg
     7801 ttttttcctt gatccattga ctgacgttgg acagggcctt gctttgctct gccttgcgtt
     7861 tttgttcctg gacaaaatct gcaaagcttt gcttgacgcc gtccttttga accttgtcct
     7921 gcaaatcatg ccatttcttt ttaaggttgc tggaagtccg ttcgtagtag tcttcaagga
     7981 tttcttcctt ggacttgtaa ttgcggtaga aggcattgcg tgacacacca gcttttcgga
     8041 ccaattcaga taccgaaatt tgcttgagct cctttttctc taggagaaat agcagagcag
     8101 tttctattga ttccttagtc agttgattcg cttctttatt tgattgatag aggtttttca
     8161 aagattgggg tgaaatttta cgacctgcca tagttttcct tttccgagtg tcacatctga
     8221 aagaaaatgc tttcagatgg ggtagtttaa tgatataatt gtaagtaaat agagatttat
     8281 tgtcaaataa aaaaataaaa ttggaaatga gaatattatg acaaaatgga aaatcgttgc
     8341 agattcgggc tgcgactatc gtcaattagc taatctagct ccggacactg aatttattag
     8401 tgtgccactt accatccaag ttggagaaca agcatttgtt gatgatgcaa gcttggatat
     8461 tgatcacatg atggaggtga tggaagcatc taagtctgca gcggggtcgg cgtgcccaag
     8521 tccgcaggct tatcaggcag cttttgaggg agctgagaac attatcgttg tgacgattac
     8581 aggtgggcta tcgggtagtt ttaatgcggc acgtgtagct agggatatgt atatcgaaga
     8641 gcatccgaat gtcaatatcc atttgataga tagtttgtca gccagtgggg aaatggattt
     8701 acttgtagac gaaatcaatc gcttaattag tgcaggatta gattttccac aagtagtaga
     8761 agcgataact cactatcggg aacacagtaa gctcctcttt gttttagcga aagttgataa
     8821 tcttgttaag aatggaagac tgagcaaatt ggtaggcact gtcgttggtc ttctcaatat
     8881 ccgtatggtt ggtgaggcaa gtgctgaagg aaaattagag ttgcttcaaa aggcgcgtgg
     8941 tcataagaaa tctgtgacag cagcctttga agaaatgaaa aaagcaggct atgatggtgg
     9001 tcgaattgtt atggcccacc gcaacaatgc taagttcttc caacaattct cagagttggt
     9061 aaaagcaagt tttccaacgg ctgttattga cgaagttgca acatcaggtc tatgcagttt
     9121 ttatgctgaa gaaggtggac ttttgatggg ctacgaagtg aaagcgtgat tcacagagta
     9181 ataattttgg gctgtaattt ccgctataga ataatccccc tcgtcttcta agttcgaggg
     9241 ggattgtttg tatgagacta ttggatttca ttcattcaaa tatcttacga attgctccag
     9301 tttatctgca aaatcttgtt caaagaagat ctgtaagaaa tcagctttct gtccgctgaa
     9361 ataataacat tttccaaaca tgtgttggat gctaggagaa agaatcccct tgcttagctg
     9421 aaaggtcacg ctccctttgg aaattcgata cgggatgttt aaagcgtatt tctctagaca
     9481 gtcttttatt ttattccatt gagcgtgata aatgtgatga agatgctgtg tgttccgcgc
     9541 aaacataccg ttatcaatgt agagcgagag agctttttgc atgataagat tggtatcgta
     9601 gtcgattaga ctcttatgtt tgatgaagat atcacgtagc tgattaggaa ggctgattgc
     9661 accgattcgg agggcaggaa agagtgtcgg tgtaaaagat tttatataga tgacgcgatt
     9721 atctgtatca agatagtgta aaggtaggct atgactagag tcgaaatctg ctaaatagtc
     9781 atcctcaatg atgtagacat cgtattgctt tgctaatttt acgatggctg tttttgttgc
     9841 tatatcatag gttgaaccga gagggttgtg caagcgagga attgtgtaga aaaacttaat
     9901 ttttccagtt tggaagatac tttccaattc ttctaggtca attccatcta aattccgttc
     9961 aattgtttga taggggattc cttgatgtcg aatgagctct atcattcgtg aataggtagg
    10021 gttctctatc aagatttccg tttttccagc caaggtttcc atttgtgtga gaatatatag
    10081 agcttgttga ctaccagctg tgataaccag ctggtctttt tttgtataga catgatagtc
    10141 cattaacaga ctttgaacgg aggaaatcaa ttctgccaat ccctcttgct ggtgatagta
    10201 gttgaatagg taattttccc gcccaataag actttctttt agacaaatcc gaaaatcttc
    10261 atagggtaat tcttgaaagt ctgtaggatt gagctctaca gtatggtctt ggaaatctct
    10321 atcctctaag atataataac cgcttttttc gacagcgtag atcttatttt ggtattttaa
    10381 ttccaacata gccttttgga cagtgtcttt gctacaatga tattgctcgc ggagttgacg
    10441 gatagaaggt aatttctctc cacgtttgaa tcgatgttcc tctattccag tcaaaatatc
    10501 ttggatgata acttgatatt ttttcatcta ggtccccttt tttatagact atgttactag
    10561 ctagtatata gaaaaaattg aagaaagaca atatatgaat aatggggttg aggttcagga
    10621 attaagctac tctatggtat aattaagtga tgaaaataat tatacctaat gcaaaagaag
    10681 taaatacaaa tctagagaat gcctcgtttt atctcctgtc tgatcgaagc aagccggtgc
    10741 tggatgccat aagtcaattt gatgtaaaaa agatggctgc cttttataaa ttgaatgaag
    10801 caaaggctga gttagaagct gaccgttggt atcgaatcag gacaggtcaa gcaaaaacct
    10861 atccagcctg gcagttatat gatggtctca tgtatcgtta tatggatagg cgaggtatag
    10921 attcgaaaga agaaaattat ctacgtgacc acgttcgtgt agcgacagcc ttatacggat
    10981 tgattcatcc ttttgaattc atttcacctc accgcttaga ttttcaaggg agcttaaaga
    11041 taggcaatca gtctttgaaa cagtactggc gaccgtatta tgaccaagaa gttggtgatg
    11101 atgaactgat tctctcactg gcttcgtcag aatttgagca ggtgttttct ccacagattc
    11161 agaaaagatt agttaaaatt cttttcatgg aagaaaaagc aggtcagcta aaagttcact
    11221 cgactatatc aaaaaaaggc agaggaagat tgctgtcctg gttggctaag aacaatattc
    11281 aggaattatc ggacattcaa gattttaagg tggatggctt tgaatattgt acttccgaat
    11341 caacggcaaa ccaacttacc ttcatacgat caataaaaat gtgaaattat gaaaaagata
    11401 acgttttcca gcgctaaaaa gggtagaaaa atattaattt ctatgatata atggatgcgt
    11461 tataggtaaa agtctaggaa ggttgtttat gaaaaagaga agcggacgaa gtaagtcgtc
    11521 caagttcaaa ttggtaaatt ttgcgctttt gggactttat tccattactc tatgtttgtt
    11581 cttagtgacc atgtatcgct ataacatcct agatttccgg tatttaaact atattgtgac
    11641 gcttttgcta gtaggagtgg cagtattggc tggattattg atgtggcgta agaaagcgcg
    11701 catatttaca gcgctcttac ttgttttttc actggtcatc acgtctgttg ggatctatgg
    11761 aatgcaagaa gttgtaaaat tttcaacacg actaaattca aattcgacat tttcagaata
    11821 tgaaatgagt atccttgtcc cagcaaatag tgatattacg gacgttcgtc agcttactag
    11881 tatccttgct ccagccgaat acgaccaaga taacatcacc gctttattgg atgacatatc
    11941 caaaatggaa tctactcaac tagcaactag ccccgcgacc tcttacctga cagcatatca
    12001 atctatgttg aatggcgaga gtcaagcgat ggtgttcaac ggagttttta ccaatatttt
    12061 agaaaatgaa gatccaggct tttcttcaaa agtgaaaaaa atatatagtt tcaaagtgac
    12121 tcagactgtt gaaacagcta ctaagcaggt gagtggagat agctttaata tctatattag
    12181 tggtattgat gcttatggac cgatttctac ggtctctcgt tcagatgtca atatcattat
    12241 gactgtcaat cgtgcgacac ataagatttt attgacaact actccacgag attcatacgt
    12301 tgctatcgca gatggcgggc aaaatcaata cgataaacta acacatgctg gtatctacgg
    12361 tgtcaatgct tctgtgcaca ccttagaaaa tctctatggg attgacatta gcaattatgt
    12421 gcggttgaac ttcatttcct tccttcaatt aatcgacttg gtgggtggaa ttgatgtata
    12481 taacgatcaa gaatttacaa gtttacatgg gaattatcat ttccctgttg gacaagttca
    12541 tttaaactca gaccaagcat taggcttcgt tcgagagcgc tactctttaa cagggggtga
    12601 caatgaccgt ggtaaaaacc aggaaaaagt gattgctgcc ttgattaaaa agatgagtac
    12661 gccagagaat ctaaaaaatt accaggcaat cctatctgga ttggaaggct caattcaaac
    12721 ggatttgagc ttagaaacga ttatgagttt agtgaatacc caactagaat caggaacaca
    12781 atttacagta gagtcacaag cattgacagg aacaggacgc tcagacttat cttcttatgc
    12841 gatgcctgga tcacaacttt atatgatgga aattaaccaa gatagtctgg agcaatcaaa
    12901 ggcagcgatt cagtccgtac ttgttgaaaa ataaagattt taggagaaaa tatgaacaat
    12961 caagaagtaa atgcaatcga aatcgatgtt ttattcttac taaaaacaat ttggagaaag
    13021 aaatttttaa ttctcttaac tgcagtgttg actgcggggt tggcatttgt ctacagtagt
    13081 tttttagtga cacctcaata tgactccact acccgtatct atgtagtgag tcaaaatgtt
    13141 gaagccggtg cgggcttgac taaccaagag ttacaagcgg gtacctattt ggcaaaagac
    13201 tatcgggaaa ttatcctatc acaagatgta ttgacacaag tagcaacgga attgaatctg
    13261 aaagagagtt tgaaagaaaa aatatcagtt tctattcctg ttgatactcg tatcgtttct
    13321 atttctgtgc gtgatgcgga tccaaatgaa gcggcacgta ttgcaaatag ccttcgcacc
    13381 tttgcagtgc aaaaggttgt tgaggtcacc aaggtaagcg atgtgacgac acttgaagaa
    13441 gcagtcccag cggaagaacc aaccactcca aatacaaaac gaaatatctt gcttggttta
    13501 ttagctggag gtatcttggc aacaggtctt gtactggtta tggaggtttt ggatgaccgt
    13561 gtaaaacgtc ctcaggacat cgaagaggta atgggattga cattgctagg tatagtacca
    13621 gattcgaaga aattaaaata ggagaacaat atggcgatgt tagaaattgc acgtacaaaa
    13681 agagagggag taaataaaac cgaggagtat ttcaatgcta tccgtaccaa tattcagctt
    13741 agcggagcag atattaaggt tgttggtatt acctctgtta aatcgaatga aggtaagagt
    13801 acaactgcgg ctagtctcgc tattgcctat gctcgttcag gttataagac cgtcttggtg
    13861 gatgcagata tccgaaattc agtcatgcct ggtttcttca agccaattac aaagattaca
    13921 ggtttgacgg attacctagc agggacaaca gacttgtctc aaggattatg cgatacagat
    13981 attccaaact tgaccgtaat tgagtcagga aaggtttctc ccaaccctac tgccctttta
    14041 caaagtaaga attttgaaaa tctacttgcg actcttcgtc gctattatga ttatgttatc
    14101 gttgactgtc caccattagg actggtaatt gatgcagcta tcattgcaca aaaatgtgat
    14161 gcgatggttg cagtagtaga agcaggcaat gttaagtgct catctttgaa aaaagtaaaa
    14221 gagcagttgg aacaaacagg cacaccgttc ttaggcgtta tcttgaacaa atatgatatt
    14281 gccactgaga agtatagtga atacggaaat tacggcaaaa aagcctaatt tctcagataa
    14341 cataagtttg ataagtaggt attaatatga ttgatatcca ttcgcatatc atatttggtg
    14401 tggatgacgg tcccaaaact attgaagaga gcctgagttt gataagcgaa gcttatcgtc
    14461 aaggtgttcg ctatatcgta gcgacatctc atagacgaaa agggatgttt gaaacaccag
    14521 aaaaaatcat catgattaac tttcttcaac ttaaagaggc agtagcagaa gtttatcctg
    14581 aaatacgatt gtgctatggt gctgaattgt attatagtaa agatatctta agcaaacttg
    14641 aaaaaaagaa agtaccaaca cttaatggct cgtgctatat tctcttggag ttcagtacgg
    14701 atactccttg gaaagagatt caagaagcag tgaacgaaat gacgctactt gggctaactc
    14761 ccgtacttgc ccatatagag cgttatgatg ctctggcatt tcagtcagag agagtagaaa
    14821 agctaattga caagggatgc tacactcagg taaatagtaa ccatgtgttg aagcctgctt
    14881 taattggcga acgagcaaaa gaatttaaaa aacgtactcg atatttttta gagcaggatt
    14941 tagtacattg tgttgctagc gatatgcata atttatatag tagacctccg tttatgaggg
    15001 aggcgtatca gcttgtaaaa aaagagtatg gtgaggatag agcgaaggct ttgttcaaga
    15061 aaaatccttt gttgatattg aaaaatcaag tacagtaacc tcatagaaat agtggaggag
    15121 ctatgaatat tgaaatagga tatcgccaaa cgaaattggc attgtttgat atgatagcag
    15181 ttacgatttc tgcaatctta acaagtcata taccaaatgc tgatttaaat cgttctggaa
    15241 tttttatcat aatgatggtt cattattttg cattttttat atctcgt
//
LOCUS       DBNFCK010000038        15061 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.038, whole genome shotgun sequence.
ACCESSION   DBNFCK010000038 DBNFCK010000000
VERSION     DBNFCK010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 15061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15061
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.038"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            32..622
                     /locus_tag="AC1PIW_000943"
     CDS             32..622
                     /locus_tag="AC1PIW_000943"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIP6605894.1"
                     /translation="MSNMYYEKNPTIAHDIHEIHVNLLDTPMSFMTDAGVFSKKMVDY
                     GSQVLLKNLYFEKGASVLDVGCGYGPIGLTLAKVFSTKTTLIDINSRALDLATKNAER
                     NGVIAKIYQSNIYENVDETFNHIVSNPPIRAGKSVVHEVIAGAFQRLKAHGTLSIVIQ
                     KKQGAPSAKAKMEEVFGNCQIIKKDKGYYILESVKE"
     gene            619..1896
                     /locus_tag="AC1PIW_000944"
     CDS             619..1896
                     /locus_tag="AC1PIW_000944"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HIP6605895.1"
                     /translation="MRAVDLIQKKRDGLELSSEEIKWLIDGYVAGTVPDYQMSALAMA
                     IYFKGMSTREISDLTMSMVATGEQIDLSAIPGIKVDKHSTGGVGDKVTLILAPLVASF
                     DIPVAKMSGRGLGHTGGTLDKLESIKGYQIEVSQADFIKQVQDTGVAVIGQSDNLVKA
                     DKLLYALRDVTATVDIIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVEEARVLA
                     QTMVDLGKAVGRKTVAVLTDMSQPVGTSIGNRLEILEAIEILQGKGREDVTEFICELA
                     QIMLGLANVEKSIEEVRQHIDNGAALKKFEEMVRAQGGDLEDLYRPVQVTQQVPVLAE
                     EDGYIVGLPALEFGLFAMKLGAGRAVKTDNLDYETGIVFHKKVGEAVSKGEQIATIYA
                     NENISENMLTNFQKNVKIDKESVKTKEIIEIIS"
     gene            1912..2574
                     /gene="deoC"
                     /locus_tag="AC1PIW_000945"
     CDS             1912..2574
                     /gene="deoC"
                     /locus_tag="AC1PIW_000945"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIP6605896.1"
                     /translation="MKLNKYIDHTILKPETTQEQVEKILAEAKEYDFASVCVNPTWVA
                     LAAESLKDSDVKVCTVIGFPLGANTPAVKAFETKDAISNGADEIDVVINIGALKTGNY
                     DLVLEDIKAVVAASGDKLVKVIIEACLLTDDEKVKACQLSQEAGADYVKTSTGFSTGG
                     ATVADVALMRKTVGPDMGVKASGGARSYEDAIAFIEAGASRIGASSGVAIMNGAQADG
                     DY"
     gene            2561..2950
                     /locus_tag="AC1PIW_000946"
     CDS             2561..2950
                     /locus_tag="AC1PIW_000946"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HIP6605897.1"
                     /translation="METIDLIELAVKNSQKAYVPYSKFPVSAVLVAKTGEIFTGVNIE
                     NASFGLTNCAERTAIFKAISEGVKDFSEIIIYGKTEKPISPCGACRQVMAEFFDKDLK
                     VTLVAKDKSTVETTVGELLPYSFTDLT"
     gene            3031..4098
                     /locus_tag="AC1PIW_000947"
     CDS             3031..4098
                     /locus_tag="AC1PIW_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family lipoprotein"
                     /protein_id="HIP6605898.1"
                     /translation="MNKKLVGLGLAAAAAVSLAACGNRASKSSSSEASSSADTSVKAA
                     IVTDIGGVDDRSFNQSAWEGLQEWGKAASLSKGNGYDYFQSASESDYITNLDSAVAGG
                     YNLVFGIGFALESAIAEVAPNNPNTHYVIVDSVVPDQDNVVSVGFADHEASYLAGVAA
                     AKSTKTNHVGFIGGMEGVIIDRFEAGFVAGAKSVNKDIKITVDYAGSFGDAAKGQTLA
                     AAQYAAGADVIFHASGGTGNGVFAAAKAENETRNEADKVWVIGVDRDQSAEGTYTSKD
                     GKSSNFVLASTLKQVGTSVKDIATKAAAGDFPGGQVLQFSLKDKGVELAETNLSEDAS
                     KAVADAKQAILDGKVEVPEKP"
     gene            4293..5828
                     /locus_tag="AC1PIW_000948"
     CDS             4293..5828
                     /locus_tag="AC1PIW_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6605899.1"
                     /translation="MTKDYVIEMRNITKIFGEFVANDHINLNVRRGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIVINGQSVKIDSPSKAAQLGIGMVHQHFMLVDAFTVAENI
                     ILGSETTKGGVIDLKKAIAEIKELSAKYGLEVDPTAKVADISVGAQQRVEILKTLYRG
                     ADLLIFDEPTAVLTPAEIAELLKIMKALVEEGKSIILITHKLDEIRAVADSVTVIRRG
                     KSIETVAVEGATNQDLAEWMVGRSVSFKTEKVEASPKEVILSIKDLVVNENRGIPAVK
                     GLSLDVRAGEVVGIAGIDGNGQSELIQAITGLRKVKSGSITIKGEEVVGKTPRKITEM
                     QVSHVPEDRHRDGLVLPMTVAENFALQTYYKEPISKNGILNYNIINEKARALMEEFDV
                     RGASELVPIKALSGGNQQKAIIAREIDRNPDLFIVSQPTRGLDVGAIEYIRKRLIAER
                     DKGKAVLVVSFELDEILDVSDRIAVIHDGVIHGIVEPAKTNKQELGVLMAGGHIEKGE
                     EHA"
     gene            5821..6882
                     /locus_tag="AC1PIW_000949"
     CDS             5821..6882
                     /locus_tag="AC1PIW_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6605900.1"
                     /translation="MLNKYKNLALPLMAVVSGILLGAIVMLVFGYDPIWGYEELFYSA
                     FGNIKSIGEIFRAMAPLVFTALGFAVASRAGFFNVGLSGQAYVGWVFAGWFALANPDL
                     PRPVLILGTVLIAMLAGGIAGAIPGILRAYLGTSEVIVTIMMNYILLYSCNYIIRDVF
                     ADSLMKNTDSTVNVSANASYQTEWLRALTDNSRMNIGIFFAIIAVFVIWFLLTKTTLG
                     FEIRSVGLNPTASDYAGMSAKRTIILSMIISGALAGLGGAIQGLGTFQNVYIQSGNLD
                     IGFNGMSVALLASNSPLGIPLAAFLFGVLSVGAPGMVRAQIPPELINVVTASIIFFIG
                     VKFIFEQLLKSRNKAKGAK"
     gene            6884..7837
                     /locus_tag="AC1PIW_000950"
     CDS             6884..7837
                     /locus_tag="AC1PIW_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6605901.1"
                     /translation="MNVSILALLISQMLIYSAPLIFTSLGGVFSERGGIVNVGLEGIM
                     VIGAFAGVVFNIEFAGTFGNATPLLAVIVGGLAGVVFAAIHAMATINFRADHVVSGTV
                     LNLLAPALGVFLVKVIYNKGQTDSITESFGKFSFPILADIPIIGEIFFKNTSLMGYVA
                     IATAFLAWFILYKTKFGLRLRSVGEHPQAADTLGINVYAMRYAGVLIAGFLGGVGGAV
                     SAQSVNINFSATTIVGSGFIALAAVIFGKWNPIGAMLASLFFGLSQSLAVVGGQLPGL
                     KDIPTVYLQIAPYLITVIALSAFFGKAVAPKTDGINYIKSK"
     gene            7993..8523
                     /locus_tag="AC1PIW_000951"
                     /pseudo
     CDS             7993..8523
                     /locus_tag="AC1PIW_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940825.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
     gene            complement(8566..9549)
                     /locus_tag="AC1PIW_000952"
     CDS             complement(8566..9549)
                     /locus_tag="AC1PIW_000952"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HIP6605902.1"
                     /translation="MTATKQHKKVILVGDGAVGSAYAYALVNQGIGQELGIIDINKDR
                     TQGDAEDLSHALAFTFPKKIYSAEYSDAHDADLVVLTAGLPQKPGETRLELVEKNLRI
                     NQQIVTEIVNSGFNGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQAL
                     AEKIGIDARSVHAYIMGEHGDSEFAVWSHANVAGVKLYDWLQDNRDIDEQGLVDLFVS
                     VRDAAYSIINKKGATYYGIGVALARITKAIFDDENAVLPLSVYQAGQYEGVEDVFIGQ
                     PAIIGAHGIVRPVNIPLSDAELQKMQASAKQLKDIIDDAFANPEIAAGVKN"
     gene            9738..12182
                     /gene="gyrA"
                     /locus_tag="AC1PIW_000953"
     CDS             9738..12182
                     /gene="gyrA"
                     /locus_tag="AC1PIW_000953"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIP6605903.1"
                     /translation="MQDKNLVTVNLTNEMKSSFIDYAMSVIVSRALPDVRDGLKPVHR
                     RILYGMNELGITPDKPHKKSARITGDVMGKYHPHGDSAIYEAMVRMAQWWSYRHMLVD
                     GHGNFGSMDGDGAAAQRYTEARMSKIALEMLRDINKNTVNFADNYDASEREPEVLPAR
                     FPNLLVNGTTGIAVGMATNIPPHNLGETIEAVKLVMDNPEVTTREIMEVLPGPDFPTG
                     ALVMGKSGIHRAYETGKGSIVLRSRTEIEEYGNGRERIVVTEFPYMVNKSKVQEHIVK
                     LVQEKRIEGITAVRDESNREGVRFVIEVRRDASANVILNNLFKQTQLQTNFSFNMLAI
                     QNGVPKILSVRQILESYIEHQKEVVTRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRNSETDAIAQAELMEKFDLSERQSQAILDMRLRRLTGLERDKIQSEYDELVALIAD
                     LADILAKPERVVQIIKDELEEVKRKYADPRRTELMVGEVLSLEDEDLIEETDVLITLS
                     NQGYIKRLAQDEFQAQKRGGRGVQGTGVKDDDFVRELVSTSTHDRLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLEENEAIQTIINVTKDQEADSYLFFATRQGVVKRTS
                     VSEFANIRQSGLKALNLKEDDELINVFLTNGQADIIMGTKFGYSVRFTETDVRNMGRT
                     ATGVRGINLREGDQLVGATMISDDQEVLVLTEKGFGKRTPASEYPTKGRGGKGIKTLK
                     VADKNGSLAGLTTVSGDEDIMVITDTGVIIRTSVANISQTGRSTMGVKVMRLNGEAKI
                     MTFALVDAAEIKEEKE"
     gene            12189..12938
                     /locus_tag="AC1PIW_000954"
     CDS             12189..12938
                     /locus_tag="AC1PIW_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HIP6605904.1"
                     /translation="MSKRENKKKRKGSFWRNLLTVVLILISLALIFNTSIRNFIIGWN
                     TNKYQISNVTTEDIEKNKQAETTFDFEQVQSISTEAILAAQWDAQRLPVIGGIAVPEL
                     GINLPIFKGVFNTSLMYGAGTMKENQEMGKGNYALASHHIFGVTGAADVLFSPLDRAK
                     NGMKIYITDKTNVYTYVIDSVEIVSPESVYVIDDVEGRTEVTLVTCTDYYATQRIVVK
                     GVLESTTPYNETAKDILDSFNKSYNQYDYGQ"
     gene            13051..13731
                     /gene="radC"
                     /locus_tag="AC1PIW_000955"
     CDS             13051..13731
                     /gene="radC"
                     /locus_tag="AC1PIW_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HIP6605905.1"
                     /translation="MYQIEFKEEAFLPRERLVEVGAERLSNQELLAIFIRTGTKKEPV
                     SILSNKLLNRLESLAALRELSIEELQSLTGIGRVKAIEIKAMIELGKRINQSELLLNE
                     RILGSEKLGRKMIHEIGHKKQEHLVALYLNTQNQIISQKTIFIGSVNRSIAEPREILH
                     YAVKCMATSIIIVHNHPSGSVQPSRNDLLFTENLKESCEKLGLVLLDHLIVGNKDYYS
                     FREESELF"
     gene            complement(13728..14420)
                     /locus_tag="AC1PIW_000956"
     CDS             complement(13728..14420)
                     /locus_tag="AC1PIW_000956"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIP6605906.1"
                     /translation="MKKPVIGISGNEYKTGDHTEPLLSYTQTCLVQAIEDAGGLPLIL
                     PATEPDLAKYYIHLIDKLILTGGQNVQPSYYHEERTIDSDNYLPKRDEFELALIRAAQ
                     ENQKPIFGICRGLQLYNVAQGGSLHQSISEHWQDIDGQEVSQTIQLTQNSPLYDIYES
                     DPSVNSFHRQAIKDLAPDLEIIALSDNQQIIEAVHSAYPTKFLGVQWHPELLYGKREI
                     EKDLFHYIVNKL"
     gene            complement(14424..>15061)
                     /locus_tag="AC1PIW_000957"
     CDS             complement(14424..>15061)
                     /locus_tag="AC1PIW_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HIP6605907.1"
                     /translation="KNDKKSDIPRATAKRLSLYYRIFKRFYAGNIEKASSKEIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYNVKELMDFFADILNDTSITNVMLVGVGNMGRALLH
                     YRFHERNKMKIVMAFEADDNPAVGTTDENIPIHAISEIKERISEANSQTAILTVPSVK
                     AQEVTDILVEAGVKGILSFSPVNLSVPKDVVVQYVDLTSELQTLLYFMRKG"
BASE COUNT         4403 a         2614 c         3491 g         4553 t
ORIGIN      
        1 aaacgttttt aggaaatgtt ataataagta catgtccaat atgtattacg aaaaaaatcc
       61 tacaatagca catgatattc atgaaatcca cgtaaactta ctagacaccc caatgtcttt
      121 catgacagat gcaggtgttt ttagcaagaa gatggttgat tatggtagtc aagtgctttt
      181 aaaaaacctt tattttgaaa aaggggcatc agtgttggat gttggctgtg gttatggacc
      241 aattggtttg acacttgcta aggtgtttag taccaagaca acactgattg atattaatag
      301 cagagctttg gatttagcta caaaaaatgc agagaggaat ggtgttatcg ctaaaattta
      361 tcaatcaaat atctacgaga atgtggacga aacatttaat catattgttt caaatccacc
      421 tattcgggcc ggaaaatcgg ttgtacatga agtaattgct ggggcttttc aacgcttaaa
      481 ggcacacgga accttgtcca ttgtcattca aaagaaacaa ggcgcaccga gtgctaaagc
      541 taagatggaa gaggtgttcg gcaactgcca gattataaag aaagataagg gttactacat
      601 ccttgaaagt gtgaaagaat gagagcagtt gatttaattc aaaaaaagcg tgatggctta
      661 gaactatcgt ctgaagaaat caaatggttg attgatggat atgtagctgg gacagtaccg
      721 gattatcaaa tgtcagcctt agcgatggcg atttatttta aagggatgtc aacgcgtgag
      781 atttctgatt tgacgatgtc catggttgca acgggtgaac agattgattt gtctgcaatt
      841 ccaggcatca aggtagataa gcattctaca ggtggtgtcg gagataaggt cacactcatc
      901 ttagcgccat tggtagcaag ttttgatatt ccagttgcaa agatgagcgg tcgtgggctt
      961 ggtcatactg gtggaacctt ggataagtta gagtcaatca aaggatacca gattgaagtt
     1021 agtcaagcag atttcatcaa gcaagttcaa gatactggtg ttgcagttat tggtcagtca
     1081 gataatcttg tcaaagctga taaactactc tatgcccttc gtgacgtgac agcaaccgtt
     1141 gacattattc cgctgattgc cagctcagta atgtctaaga aaattgccgc aggtgcggat
     1201 gcgattcttc tagatgtcac ggtgggagaa ggtgccttca tgaagactgt tgaagaagca
     1261 cgggttctag ctcaaactat ggtggatctt gggaaagcgg ttggtcgtaa gacagttgct
     1321 gttttaactg acatgtctca accagttgga acttcgattg gaaatcgttt ggaaatttta
     1381 gaggctattg aaattttaca gggtaaaggt cgagaagatg ttaccgaatt tatctgtgaa
     1441 ttagcccaaa ttatgttggg gcttgctaat gttgagaaaa gcattgaaga agtacgtcaa
     1501 catattgaca atggtgcagc attgaagaaa tttgaagaga tggtccgtgc tcaaggtggt
     1561 gacttagaag atttatatcg cccagttcag gttacacagc aagttccagt tcttgcggaa
     1621 gaggatggct atattgtggg acttcctgct ttggaatttg gtctctttgc aatgaaactt
     1681 ggtgcaggtc gtgcagtcaa aactgacaat ttagattatg aaacgggtat tgttttccat
     1741 aagaaagtgg gggaagctgt ttctaaaggc gaacaaatcg caacgattta tgccaatgaa
     1801 aatatttcgg aaaacatgct tacaaatttc caaaaaaatg ttaaaataga taaagaaagt
     1861 gtaaaaacaa aagaaatcat cgagattatt tcttaatgta cggagaaagc tatgaaattg
     1921 aataaatata ttgaccatac aatcttgaaa cctgagacaa cacaagagca ggttgaaaaa
     1981 attttagcag aagcaaaaga atatgatttt gcaagtgtct gtgttaaccc aacttgggtt
     2041 gctttagcag ctgaaagctt gaaagatagt gatgtaaaag tttgtacggt tattggtttc
     2101 cctctcggag caaacacacc tgctgtaaaa gcgttcgaaa caaaagatgc aatttcaaat
     2161 ggagcagatg agattgacgt ggttatcaat atcggtgctc tgaaaactgg aaattacgat
     2221 ttggttttgg aggatattaa agctgttgta gcagcaagtg gtgataaact agtcaaagtt
     2281 atcatcgaag catgcctcct tactgatgat gagaaagtga aagcttgtca attatctcaa
     2341 gaagctggtg cggactatgt taaaacatct acaggatttt ctacaggcgg tgctacagta
     2401 gcggatgttg ccttaatgag aaaaacagtg ggtcctgaca tgggagtgaa ggcttcaggt
     2461 ggtgcacgtt cgtatgaaga tgccattgcc tttatcgaag caggagcgtc tcgtattggt
     2521 gcgtcatcag gcgtggcaat catgaatgga gcacaggcgg atggagacta ttgatttaat
     2581 tgaattagct gtaaaaaaca gtcaaaaagc atacgtaccg tactcaaaat ttccagttag
     2641 tgcagttctt gtagctaaga caggggaaat atttacgggt gtaaacattg aaaatgcaag
     2701 ctttggtttg accaattgcg ctgaaaggac agctattttc aaggccattt ctgaaggtgt
     2761 gaaagatttt tctgaaatca ttatttatgg taaaacagaa aaaccaatat ctccttgtgg
     2821 agcttgtcgc caagttatgg cagaattttt tgataaggat ttaaaagtga ccttggtcgc
     2881 taaagataaa tcgacagtcg agacgacagt cggggaatta ctaccatatt cctttacaga
     2941 cttaacgtag tctgtatgac agtcatttga ctgtagcaat attactctgc aacaatgtag
     3001 ttgcaaaaag atttttagga gggttcagaa atgaacaaga aacttgttgg actgggtcta
     3061 gcagctgctg cggctgtatc tttggctgca tgtggtaacc gtgcttcaaa aagcagtagc
     3121 tcagaagcat cttcatcagc ggacacatct gtgaaagctg ctatcgttac tgacattggt
     3181 ggtgttgatg accgttcatt taaccaatct gcttgggaag gtcttcaaga gtggggtaaa
     3241 gcagctagtc tttccaaagg taatggctac gattacttcc aatcagctag tgaatcagat
     3301 tatatcacaa accttgattc agctgttgct ggtggttaca atcttgtgtt cggtattggt
     3361 tttgccttgg agagtgcgat tgctgaggtt gcaccaaata atcctaatac acactatgta
     3421 atcgtggata gtgttgttcc agatcaagac aatgtagtta gcgtaggttt cgcagaccat
     3481 gaagcatcat atcttgctgg tgttgctgca gctaaatcaa ctaaaacaaa tcacgtaggt
     3541 ttcattggtg gtatggaagg tgttatcatc gatcgctttg aagctggttt cgttgctggt
     3601 gctaagtctg taaacaaaga catcaagatt acagttgact atgcgggttc atttggtgat
     3661 gctgctaaag gtcaaactct tgcagctgct caatatgctg caggtgcaga tgttattttc
     3721 cacgcttctg gtggtactgg taacggtgta tttgcagctg ctaaagcaga aaacgaaact
     3781 cgtaatgaag cagataaagt ttgggtaatc ggtgttgacc gtgaccaatc agcagaaggt
     3841 acatacactt ctaaagatgg taaatcttct aactttgtat tggcttcaac attgaaacaa
     3901 gttggtactt cggttaaaga tattgcaact aaggcagctg caggtgattt ccctggcggt
     3961 caagtacttc aattctcatt gaaagataaa ggtgttgaat tggcagaaac taatctttca
     4021 gaagatgctt ctaaagcagt tgcagatgct aaacaagcaa ttttggatgg caaagtagaa
     4081 gttcctgaaa aaccttaatg aatttctttt cgacaattcc gattcggcga cccgacgggg
     4141 cgccgttttc gggctgagat ttttgtcagt aaagcctatc agctatggta ggttttactg
     4201 acaaaaatgg tatagttgat gaagagtttt taagtactca aagtagtact gaagaatttt
     4261 cagaaggcta tagtcagaaa ggataataga ttatgactaa ggattatgtc attgaaatgc
     4321 gcaatatcac taagatattt ggtgaatttg tagcaaatga ccacatcaat ttgaatgtca
     4381 gacgtggtga aatccatgcc cttcttggtg agaatggtgc gggtaaatct actctgatga
     4441 acatgcttgc tggcttgttg gaacctacaa gcggtgaaat tgttatcaac ggtcaatccg
     4501 tgaagattga ctctccatcc aaagcagccc aattgggtat tggtatggtg caccaacact
     4561 ttatgttggt tgatgctttc acagttgcag agaacatcat tcttggttct gaaaccacca
     4621 aaggtggcgt gattgacctt aaaaaagcca tcgcagaaat taaagaactt tctgctaaat
     4681 atggtcttga agttgatcca actgcaaagg ttgcagatat ttctgtgggt gcacagcaac
     4741 gtgtagaaat ccttaaaaca ctttaccgtg gagcagacct tttaattttt gatgagccga
     4801 cagctgtatt gacccctgcg gaaattgcag agctcttgaa aattatgaaa gccttggttg
     4861 aagaaggtaa atctattatt ttgattacgc acaaactgga tgaaattcgt gcagttgccg
     4921 atagtgtaac agttattcgt cgaggtaaat caatcgaaac agttgccgtt gaaggagcaa
     4981 caaaccaaga tttggctgag tggatggttg gacgttccgt ttcctttaaa acagagaaag
     5041 ttgaagcgag tcctaaagaa gtcattttgt caattaagga tttggttgtc aatgaaaacc
     5101 gtggtattcc agcagttaag ggcttatcgc ttgatgttcg agcaggtgag gttgttggta
     5161 ttgccggtat cgacggaaac ggtcaaagtg agttgattca ggcaatcact ggtttacgta
     5221 aggtaaaatc tggctcaatc actatcaaag gtgaggaagt agttggtaaa actccacgta
     5281 aaattacaga aatgcaagtg agccacgttc ccgaagatcg tcatcgtgat ggactcgttc
     5341 ttccgatgac tgttgctgag aactttgcgc ttcaaactta ctataaagaa ccgatttcaa
     5401 aaaatggtat cttgaactat aatatcatta atgaaaaagc acgcgcgtta atggaagaat
     5461 ttgacgttcg tggggcaagt gaactcgtcc caatcaaggc tctttcaggc ggtaaccagc
     5521 aaaaagctat catcgcgcgt gaaatcgatc gcaacccaga ccttttcatt gttagccaac
     5581 caacacgcgg tttggacgtt ggtgctattg agtatattcg taagcgtctt attgcagaac
     5641 gtgataaggg taaagctgtt cttgttgtca gcttcgaatt ggatgaaatt ttggatgtat
     5701 cagatagaat tgctgttatc catgatggtg tcatccatgg tattgttgaa cctgctaaga
     5761 ccaataaaca agagttaggt gtcttgatgg caggtggaca tattgagaag ggagaagaac
     5821 atgcttaata aatataagaa tttggccctt ccattaatgg ctgtggtcag cggtatccta
     5881 cttggagcga ttgtcatgct tgtttttggc tacgatccaa tttggggcta tgaagagtta
     5941 ttctattcag cttttggtaa tatcaaatca attggtgaaa ttttccgtgc aatggcacca
     6001 cttgttttca ctgctctcgg tttcgcagta gctagtcgtg caggtttctt caacgttggt
     6061 ttgtctggtc aggcctatgt tggttgggtt tttgctggat ggtttgcctt ggcaaatcct
     6121 gacttaccac gtccagtttt gattttggga acagtcttaa ttgccatgtt ggcaggcggt
     6181 attgctggag ctattccagg tattctacgt gcctatttgg gaacaagtga agttatcgtt
     6241 acgattatga tgaactacat cttactctat tcatgtaact atatcattcg tgatgtattt
     6301 gcagatagtt tgatgaaaaa tacagactcg acagttaacg tgtcagcaaa tgcctcttac
     6361 caaacagagt ggctccgtgc attgacagat aactcacgta tgaatattgg tattttcttt
     6421 gccattattg cagtttttgt gatttggttc ctattgacca aaacgactct tggttttgag
     6481 attcgctcag tggggcttaa cccaacagca tcggattatg cgggtatgtc tgcgaaacgt
     6541 acgattattc tatctatgat tatttcaggt gctcttgcgg gtcttggagg agctatccaa
     6601 ggtttgggta ctttccaaaa cgtttatatc caaagtggta acttggatat tggttttaat
     6661 ggtatgtcag tagcgctttt agcttctaat tcaccgttag gtattccatt ggctgccttt
     6721 ctattcggtg ttctttctgt cggtgctcca ggtatggtac gtgcacagat tccacctgaa
     6781 ttgatcaacg tggttacggc atcaatcatt ttctttatcg gtgtgaaatt tatttttgaa
     6841 caattattaa aatctagaaa taaagcgaaa ggagcgaaat aagatgaatg tttcaattct
     6901 tgccttactc atttcgcaaa tgttgattta ttcagcgccc ttaatcttta ccagtcttgg
     6961 aggagttttc tctgaacgcg gtggtattgt aaacgttggt ctagaaggaa tcatggttat
     7021 cggtgccttt gctggtgttg ttttcaatat cgaatttgca gggacatttg gtaatgctac
     7081 tccattatta gctgttattg taggaggtct tgctggtgtt gtctttgctg ctattcatgc
     7141 gatggcgacg attaatttcc gtgcagacca cgttgtatca ggtacggtgt tgaacctttt
     7201 ggctccagca ttgggagttt tcctagtcaa ggttatctat aataagggtc aaactgatag
     7261 tattactgaa tcatttggta aattttcatt ccctatcttg gcagatattc caatcattgg
     7321 tgaaattttc ttcaaaaata caagcttgat gggttatgta gctattgcaa cagcatttct
     7381 tgcttggttc atcctttata aaaccaagtt cggtttgcgc cttcgttcag taggtgaaca
     7441 tccgcaggca gcggatactt tgggaatcaa tgtctacgct atgcgttatg caggtgtgtt
     7501 gattgctggt ttccttggtg gtgttggtgg agctgtaagt gctcaatcag tgaatatcaa
     7561 cttctctgca acaacaatcg taggttcagg tttcatcgct ctagcagctg taattttcgg
     7621 taagtggaat ccaattggcg ctatgcttgc cagtctattc ttcggtcttt cgcaaagttt
     7681 ggcagtagtg ggtggacaat taccaggatt gaaagatatt ccaacggttt atcttcaaat
     7741 tgcaccgtat ttgattactg ttattgctct gtctgctttc tttggtaaag cagtcgctcc
     7801 taaaacagac ggtatcaact atatcaaatc taaataaaaa ggtccagtgg acctttttat
     7861 catgttcttt gctgtacagg aaatgaataa ttgtcctatt caaaggaaga ggagctagag
     7921 ggacttttag ttgagaatag ggagaatgac tggtataata gtttgtcact aaggtatgaa
     7981 atgagattaa ttgtgagacg attggtgtta aatattgcaa tgagtttaga tggatttatt
     8041 gcacgtaagg atggaagcta cgattggatt gaagggcatg gtaccgataa atacgatact
     8101 gtcttacagt ttgataatca aaaattcttt aacaactgtg atacagttgt gatggggaga
     8161 aaatcattag aagattgtcc gttggaaatg attgaaggat atcaggaaaa gcaatttatc
     8221 gttgcgagtc ataccgagca gacggactat ggtaatgtgc gtttcgtaag ggacatcatc
     8281 gggtagatta tggaacttcg ttctagagaa ggtggagata tttggttgtt tggtggagca
     8341 agtctggttc aggactgttt agaggcggga gtgattgatc accttattat tgggattatt
     8401 cctactattt tgggtgacgg tattccactt ttcgcctcac ttttggaaga aaagaaattg
     8461 ctcttggtag agtcaactgt cacagatgga attgctatgt tacgctatga tattagaagg
     8521 taaaaaaggc gagctattgc tcaccttttt tactatttta tataattagt tttttacacc
     8581 agctgcaatt tctgggtttg cgaaagcatc atcgatgatg tctttcaatt gtttagcaga
     8641 tgcttgcatt ttttgcaatt cagcgtcact caatgggata ttgactggac gaacgatacc
     8701 gtgtgcgccg atgatagcag gttgaccgat gaagacatct tcaacacctt catattgacc
     8761 agcttggtaa actgacaatg gaagtactgc attttcatca tcaaagattg ctttagtgat
     8821 acgtgcaagt gcaacaccga taccgtagta agttgcgcct tttttgttga tgattgagta
     8881 agctgcatca cgaacagata caaacaagtc aacaagacct tgctcatcaa tatcgcggtt
     8941 gtcttgcaac cagtcgtaca atttcacacc ggcaacgttg gcatgtgacc aaactgcaaa
     9001 ttctgagtca ccgtgctcac ccatgatgta agcgtgaact gaacgtgcat cgataccgat
     9061 tttctcggcc aatgcttgac ggaaacgagc tgagtcaaga gaagtaccag aaccgataac
     9121 acgctcttta gggaatccag agaatttcca agttgagtaa gtcaatacgt caacagggtt
     9181 agcagcaaca aggaagatac cgttgaaacc agagttaacg atttctgtaa cgatttgttg
     9241 gttgatacga aggtttttct ctaccaattc aaggcgagtt tcacctggtt tttgtggcaa
     9301 accagctgtc aatacaacca agtctgcgtc atgagcatct gaatactcag cagagtagat
     9361 ttttttaggg aatgtgaagg ccaatgcgtg gctcaagtct tctgcatcac cttgagtacg
     9421 gtctttgttg atatcgatga tacccaattc ttgaccaatc ccttggttaa caagagcata
     9481 agcataagca gaacctacgg caccgtcacc gacaaggatt acttttttgt gttgtttagt
     9541 tgcagtcatt gctaaacatc tccttagttt tatttgggga tgacttcatc ccaaccataa
     9601 tacattctag catgttttaa gctttttgtc acttgtgaaa gcgcctttga aaatgtttac
     9661 atgctagttt ccaacaaaga aatttattcg ccaatatggt ataatagaag cacaatgact
     9721 gttgaaagag gcatataatg caagataaaa atttagtaac ggttaatctg accaatgaga
     9781 tgaaatcatc cttcatcgac tatgcgatga gtgttatcgt ttcacgtgct ctgcctgatg
     9841 tacgtgatgg tctgaagccg gttcatcgcc gtattttgta tgggatgaat gaattaggga
     9901 ttacaccgga taaaccacat aaaaaatcag cccgtatcac aggggatgtt atgggtaaat
     9961 atcacccgca tggagatagt gcgatttacg aagcaatggt tcgtatggca caatggtgga
    10021 gctaccgtca catgttagtt gatggtcacg gaaactttgg ttccatggat ggcgatggag
    10081 cagccgctca gcgttataca gaagcacgta tgagcaagat tgcgcttgaa atgcttcgtg
    10141 atattaacaa gaacacggta aattttgcgg ataactatga cgcgagtgaa cgtgagcctg
    10201 aggttttacc tgctcgtttc cctaaccttc tggttaacgg aacgactggt atcgccgttg
    10261 gtatggctac caacattcct ccacacaact tgggtgagac tatcgaggca gttaagctgg
    10321 ttatggacaa tcccgaggta acaacacgtg agattatgga agtcttgcct ggaccagatt
    10381 ttccgacagg tgccttggtt atggggaaat ctggaattca ccgtgcttat gaaactggta
    10441 aaggttcaat agttcttcgt tcccgcacag aaattgaaga atatggaaat ggtcgtgaac
    10501 gcattgttgt tacagagttt ccatacatgg tcaataagtc caaggttcaa gaacatattg
    10561 tcaaattagt ccaagaaaaa cgtattgaag gaatcactgc tgttcgagac gaatccaacc
    10621 gtgagggtgt acgatttgtt atcgaagttc gtcgtgatgc ttctgctaac gttattttga
    10681 ataacctatt caagcaaaca cagcttcaaa ccaactttag tttcaacatg ttggccatcc
    10741 aaaatggtgt accgaaaatt ctttctgtac gtcagattct agagtcttat attgaacacc
    10801 aaaaagaagt agtcactcgt cgtacacgat ttgataaaga aaaggctgag gcgcgtgctc
    10861 atatcttaga aggcttgctg attgctcttg accatattga tgaagtcatt cgtattatcc
    10921 gtaattcaga aactgatgct attgctcaag ctgaattgat ggagaaattt gacctttctg
    10981 aacgtcagag tcaggctatt cttgatatgc gtcttcgtcg tttgacaggt ttggaacgcg
    11041 acaagattca atcagaatat gatgagttag tggccttgat tgcagatttg gctgacattc
    11101 tagctaagcc tgagcgtgtt gttcagatta ttaaagacga attagaagaa gtcaaacgta
    11161 aatatgctga cccacgccgt acagaattga tggttggtga agttctatcc cttgaagatg
    11221 aggacttgat tgaagaaacg gatgttttaa tcaccttgtc aaaccaaggc tacatcaagc
    11281 gtttggcaca ggatgagttt caagctcaga agcgtggagg acgtggggtt caaggaactg
    11341 gcgtcaagga tgatgatttt gtacgagaat tagtctctac aagcactcat gatcgcttgc
    11401 tcttcttcac taataaggga cgtgtttacc gtctcaaagg ctatgaaatc ccagaatatg
    11461 gtcgtactgc aaagggactt ccagtggtca atttgcttaa attggaagaa aacgaggcaa
    11521 ttcaaaccat tatcaatgta actaaggacc aggaggcaga tagctatctc ttctttgcaa
    11581 cccgccaagg tgttgtaaaa cggactagtg tgtcagaatt tgccaatatt cgccagagcg
    11641 gtctgaaagc tttgaatttg aaagaagacg atgaactcat caatgttttc ttaacaaatg
    11701 gccaagcaga cattattatg gggactaagt tcggttattc tgtacgtttc actgaaacag
    11761 atgttcgtaa tatgggccgt acagcaacag gtgttcgtgg tatcaattta cgtgagggag
    11821 accagttagt tggcgctact atgatttctg atgaccaaga agtactcgta ttgacggaaa
    11881 aaggttttgg taaacgtaca ccagccagtg agtatccaac caaaggtcgt ggtggtaagg
    11941 gtatcaagac tcttaaagtt gctgataaga atggttctct ggcaggtttg acaacggtat
    12001 ctggtgatga agatattatg gttattaccg atactggtgt gattattcgt acaagtgttg
    12061 ccaatatctc tcaaactggt cgttcaacca tgggggtaaa agtgatgcgc ttgaatggtg
    12121 aagcgaaaat catgaccttt gccttggtag atgctgccga aattaaagaa gaaaaggaat
    12181 aaatagtcat gtcaaaacgt gaaaacaaga agaagcgtaa aggttctttt tggcgcaatc
    12241 ttttaacagt tgtattgata ctgatttcct tggcattgat attcaacacc tctatccgta
    12301 attttattat cggctggaat acgaataaat accagattag caatgttacg acagaggata
    12361 tcgaaaagaa taaacaggct gagacaacat ttgatttcga acaggttcag tctatttcta
    12421 cagaagctat tttagcagct cagtgggatg cacaacgctt acctgtaatt ggtgggattg
    12481 cggttcccga gcttggtatc aaccttccta tttttaaagg ggtcttcaat acttcgctca
    12541 tgtatggagc tggtaccatg aaggaaaacc aagagatggg gaaaggaaat tatgcgttgg
    12601 ccagccacca tatttttggt gtaactggtg cggcagatgt tctcttttca ccgcttgatc
    12661 gtgctaaaaa cggcatgaaa atctatatta ctgacaagac caatgtttac acctacgtta
    12721 ttgatagtgt ggaaattgtt tcgcctgaaa gtgtctatgt cattgatgat gtagaaggac
    12781 gtacagaagt tacgttagtg acatgtacgg actattatgc tacgcaacgt attgttgtaa
    12841 aaggagttct tgaatcaacc actccatata atgaaacggc aaaagacatc ttagattcct
    12901 tcaataagag ttataatcag tatgattatg gacaataaaa catctatata ttaaggataa
    12961 agaagtggta taggactgag gagataatct ttggttttat atggcttctt ttttgcaaga
    13021 ttttacgaat agataggtga ggaggaaatt atgtatcaaa ttgaatttaa agaagaggcc
    13081 tttctaccta gagaacgttt agtagaagtt ggagctgagc gtctcagtaa tcaggaacta
    13141 ctagcaattt ttattagaac tgggacaaaa aaagaacctg tttctattct ctccaataaa
    13201 ttattgaatc gtttggagag tttagcggct ctaagagaac tatcaattga agaattgcaa
    13261 agtttgactg gaattgggcg cgtcaaggcg attgaaatta aggctatgat tgaacttgga
    13321 aaacgtatca atcaatcaga gctactctta aatgagagaa ttttgggaag tgagaagttg
    13381 ggacgtaaga tgattcatga aatcgggcat aagaagcagg agcatctagt tgcactttat
    13441 ctcaatacac agaaccagat tatcagtcaa aaaacgattt ttattgggag tgttaatcgt
    13501 agcattgcag aaccacgtga aattttgcat tacgcagtaa aatgtatggc aacatcgatt
    13561 atcatcgttc ataaccaccc gtcgggttca gtacaaccta gtaggaatga tttgctattt
    13621 acggagaatt taaaagagtc ctgcgaaaag ctaggactgg tattattaga tcatttaatt
    13681 gttggaaata aggattacta ttcttttaga gaagaaagtg agttatttta aagtttattg
    13741 acaatatagt ggaagaggtc tttttcaatc tcacgtttac cgtagagtag ttcaggatgc
    13801 cattgaaccc ctaaaaattt agttgggtag gctgaatgaa ctgcttcaat aatctgttga
    13861 ttatctgata gtgcaatgat ttctaagtca ggggctaagt ctttgatagc ctgacggtga
    13921 aatgagttca cactcggatc ggactcatat atgtcgtaaa gtggactatt ttgagtcagt
    13981 tgaatcgttt gactgacttc ttgtccgtct atgtcttgcc aatgttcaga aattgattga
    14041 tgaaggctac caccttgagc tacattgtag agctgtagac ctcggcagat accgaagata
    14101 ggtttctgat tttcttgggc agcccttatg agggctaatt caaactcgtc acgctttggt
    14161 agataattat cgctgtcaat agtcctttct tcgtgatagt aacttggctg gacattttgt
    14221 cctcctgtta ggatgagttt gtcaataaga tgaatataat attttgccaa atctggctct
    14281 gtagctggaa ggatgagtgg gagcccacca gcatcttcaa tagcttgtac gaggcaagtc
    14341 tgggtataag aaagcagggg ttctgtatga tcccctgttt tatattcatt tccagaaata
    14401 ccaataactg gttttttcat tgcttatcct tttcgcataa agtagagtag tgtctgtaat
    14461 tcactagtca aatcaacata ctgaacaacg acatcttttg ggacagacaa gttaactggt
    14521 gaaaagctca gaattccctt gacaccagct tcaacaagaa tatcggtcac ttcttgtgcc
    14581 ttgacgctgg gtaccgttag aatggcggtc tgagagtttg cttcgctgat acgttccttg
    14641 atttctgaga ttgcatggat tgggatattt tcatctgtcg ttccaacagc tggattatca
    14701 tctgcttcaa aagccatgac tattttcatt ttatttcgtt cgtggaagcg atagtgtagt
    14761 aaggcccgcc ccatattccc aacacctaca agcataacat tggtgatgga ggtatcattt
    14821 agaatatccg caaagaaatc catcaactct ttgacgttgt agccaaagcc acgacgacca
    14881 agctctccaa aataggaaaa atctcggcgg acggtagctg aatcgattcc gatagcttcg
    14941 gcaatttctt ttgaactagc tttttcaata tttcctgcat agaaacgttt aaaaatgcga
    15001 tagtagaggg atagacgttt ggcagtagcg cgtggaatat cagatttttt atcgtttttc
    15061 a
//
LOCUS       DBNFCK010000039        14688 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.039, whole genome shotgun sequence.
ACCESSION   DBNFCK010000039 DBNFCK010000000
VERSION     DBNFCK010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 14688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14688
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.039"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..354
                     /locus_tag="AC1PIW_000958"
     CDS             <1..354
                     /locus_tag="AC1PIW_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605908.1"
                     /translation="ENIDEISFNSSDGRGFKVSEKDIKIFDKKEFLSSEEIQISGTIT
                     VLNTHDNTGKIQIESNEIPLGEYKFDVQKQIRYPEYLSSAMRKKLRYRCYPKINFDPS
                     TLKEKVIGVRIIEKL"
     gene            complement(417..1193)
                     /locus_tag="AC1PIW_000959"
     CDS             complement(417..1193)
                     /locus_tag="AC1PIW_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6261 family protein"
                     /protein_id="HIP6605909.1"
                     /translation="MKIRIPLYAQHLNQAEFTQLITSSVDTLKQYSSSTNDPMVTDLI
                     KQLEESLPLLKKSLKQKRGSDMTSNLNKALKNRKADYSAFVNGLKLFKNTRTPEKLQA
                     YHRLQELIKLYKNTRSTNMQESSALIDSLLARLAKPPYSEDVSLLMLDEAIANLRESH
                     ESTYRLYLKRSQDTSTREKINSLEVRKTAFNLYTLLYSHLVNKISYDPTAPEKVILQV
                     LNDIRQDFSERTRQKNQTETNSATINIVTTLPQGKEIETT"
     gene            1545..2162
                     /locus_tag="AC1PIW_000960"
     CDS             1545..2162
                     /locus_tag="AC1PIW_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5052 family protein"
                     /protein_id="HIP6605910.1"
                     /translation="MKWNKKLALSAVLVASLFTLSACQSISNWWKNTKEEWIGLEMTV
                     RTYDENSQLIDEMSGKSLSISRNQEFDSVDAEGYSNADSSVLKVTLGNYEIDHVGSSL
                     IAAEEGLEDLYAKYQTTVDIANYDRAIPLVNRMVSSLKNDFTGKAKVVLIRSQNGTPL
                     ATYVGDKVSLYASDAPKTSELLIDGKRLIIYRCDYTIYDRELLEN"
     gene            2163..2672
                     /locus_tag="AC1PIW_000961"
     CDS             2163..2672
                     /locus_tag="AC1PIW_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HIP6605911.1"
                     /translation="MIDIRSARIEDAADLVAIYAPYVEKTAITFETEVPTVEAFASRI
                     EKTLEKFPYLVAVEEGKVVGYAYASTYYARAAYDWTVELSVYIKQEARGKGIGTLLYN
                     ALEKDLTARGFKNFLACIALPNAASIALHEKRGYEQVAHFKKVGYKFDTWHDIVWLQK
                     FLVGKQNED"
     gene            2662..3315
                     /locus_tag="AC1PIW_000962"
                     /pseudo
     CDS             2662..3315
                     /locus_tag="AC1PIW_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754918.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            3324..3611
                     /locus_tag="AC1PIW_000963"
     CDS             3324..3611
                     /locus_tag="AC1PIW_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HIP6605912.1"
                     /translation="MSKVYEFETLIHPVPDKGGAYIIFPYDIREEFGKGRVKVHARFD
                     CHPYDGSIVNMGVKDEAGNICYIIGVQKAIRAAIGKQAGDRVQVTIQERME"
     gene            complement(4313..5089)
                     /locus_tag="AC1PIW_000964"
     CDS             complement(4313..5089)
                     /locus_tag="AC1PIW_000964"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HIP6605913.1"
                     /translation="MNTRKGIIFNIQHFSLHDGPGIRTTVFLKGCPLRCPWCANPESQ
                     KRKPEPMLDAVSKKMTIMGEEKSVDDIISEVMKDIDFYEESGGGLTLSGGEIFAQYEF
                     AKAILMEAKRHGLHTAIETTAFVEHHKFVDLIQYVDFIYTDLKHYNTISHRKVTGVNN
                     NLIIQNIQYAFSIGKEIVLRIPVIPDFNNSLEDAEQFACLFNQLKIDKVQLLPFHQFG
                     ENKYKLLNRTYAMTDVKALHPEDLKDYQAIFIKHNINCYF"
     gene            5221..5964
                     /locus_tag="AC1PIW_000965"
     CDS             5221..5964
                     /locus_tag="AC1PIW_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIP6605914.1"
                     /translation="MERLDKIIQLVSQHEKIDVNSLAEQLDVSKVTIRKDLDKLESKG
                     LLRREHGYAVLNSGDDLNIRMSFRYDVKKRIAKEAANLIADNETIMIESGSTCALLAE
                     EICKTKRNVTIITNSYFIANYVRQYDSCQIILLGGEFQKDSQVTVGPLLHKMIQFFHV
                     DKAFVGTDGYDSEHGFTGKNLMRSEVVQYMSDVAENMIVLTDSSKFTKRGTVRRFGLS
                     QVAQVITDTSISEDLVKELENARVTVRLV"
     gene            5977..6951
                     /locus_tag="AC1PIW_000966"
     CDS             5977..6951
                     /locus_tag="AC1PIW_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HIP6605915.1"
                     /translation="MKQEKRRQLAKIAYLYYEEGKSQAEIAADTGIYRTTVSRMLTKA
                     KSEGIVKIEIQDFDRRLYRLEKYVQEKYGLKGLELVENSTDEDQLDLENRLAQAAADM
                     LTNLIDDGKKVGFSWGRSLSLIVDKIGNHRMKGVKFVPIAGGPSHIHARYHVNTLIYD
                     MANKFRGECSFINATIIQENQELAQGILSSKYFEELRADWKDLDVAAIGIGGRVDEKN
                     RQWLDMLTTADFQALSDEGAVGETCCRGLNQYGQAVVEDLQKRTIAISLDDLKEVPDT
                     IALAYGEEKAQAILAVLRAGYIHHLVTDERTIVKVLELDNDVHVHTNL"
     gene            7139..7462
                     /locus_tag="AC1PIW_000967"
     CDS             7139..7462
                     /locus_tag="AC1PIW_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HIP6605916.1"
                     /translation="MEMIVADQLIMGLIIYAGDAKSHAYQALSLVKAGEEERAEEELE
                     LADAALLEAHNMQTKFLAQEAGGAKSDITALFVHSQDHLMTSITEINLIKEIIDLRKE
                     LKKVG"
     gene            7495..7800
                     /locus_tag="AC1PIW_000968"
     CDS             7495..7800
                     /locus_tag="AC1PIW_000968"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIP6605917.1"
                     /translation="MVKIALFCAAGFSTGMLVNNMKIAAADAGVEVEIDAHSQGKLAD
                     YLDEIDVALLGPQVAYTLDKSKALCDEKQVPIAVIPMADYGMLDGKKVLQLALSMVS"
     gene            7813..9114
                     /locus_tag="AC1PIW_000969"
     CDS             7813..9114
                     /locus_tag="AC1PIW_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HIP6605918.1"
                     /translation="MSKIDTQKIIVPIMKFVNMKGIIALKDGMLAILPLTVVGSIFLI
                     LGQLPFEGLNAAIASIFGAEWTEPFMQVYSGTFAIMGLISCFAIGYSYAKNSGVEPLP
                     AGVLSLSSFFILLKSSYIPVDGEPIGDAIAKVWFGGQGIIGAIIIGLVVGAIYTTFIQ
                     RHIVIKMPEQVPQAIAKQFEAMIPAFVIFLLSMIVYIVSKMATNGGTFIEMIYDVIQV
                     PLQGLTGSLPGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKALLAADKLS
                     LENGAHIVTQQFLDSFLIMSGSGITFGIVFAMLFAAKSKQYKALGKVAAFPALFNVNE
                     PVVFGFPIVMNPVMFLPFVLVPILAAFIVYGAISIGFMQPFSGVTLPWSTPAIISGFM
                     VGGWQGAVVQIIILAVSTIVYFPFFKIQDKIAYESETKEPV"
     gene            9266..11710
                     /locus_tag="AC1PIW_000970"
     CDS             9266..11710
                     /locus_tag="AC1PIW_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HIP6605919.1"
                     /translation="MKTVTEVAGTSIKTEYFGSLTDRMNKYREDVLDKKPYIDAERAV
                     LATKAYQEHREKPNVLKRAYMLKEILENMTLYIEEESLIAGNQASSNKDAPIFPEYTL
                     EFVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWEKNNLRSRAGVLLPEEVQVYMET
                     GFFGMEGKMNSGDAHLAVNYQKVLEQGLRGFEERVRVAKANLDLTDPASIDKYHFYDS
                     IFIIIDAVKAYADRFVALATELAEKAPTAQRRQELLEIARICAKVPYEPAETFAEAVQ
                     SVWFIQCILQIESNGHSLSYGRFDQYMYPYVKADLEAGRETEDSIVERLTNLWIKTIT
                     INKVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTV
                     RYHAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIEKGVLEEDAYDYSAIGCVET
                     AVPGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPAFGHFKDMKSFEVLETAW
                     EKTLRHLTRMSVIVENAIDIALEREVPDILCSALTDDCIGRGKHLKEGGAIYDYISGL
                     QVGIANLSDSLAAIKKLVFEEGRLTPEELWHALETDYAGERGKEIQEMLIEDAPKYGN
                     DDDYADQLVTAAYDIYINEIAKYPNTRFGRGPIGGIRYSGTSSISANVGQGRGTLATP
                     DGRNAGTPLAEGCSPSHNMDKNGPTSVLKSVAKLPTHEIVGGVLLNQKVNPQTLAKEE
                     DKQKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQLHPEKHRDLIVRVAGYSAFFNVL
                     SRATQDDIIGRTEHTL"
     gene            11725..12393
                     /locus_tag="AC1PIW_000971"
     CDS             11725..12393
                     /locus_tag="AC1PIW_000971"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HIP6605920.1"
                     /translation="MEFMLDTLNIEEIRKWAEVLPLAGVTSNPTIARKEGDIDFFERL
                     HLIRDIIGPNASLHVQVVAKDYEGILADAKKIRELAPENIYIKVPVTPAGLAAMKTLK
                     AQGYQITATAIYTVFQGLLAIEAGADYLAPYYNRMANLNIDSNAVIAQLSEAIDRECS
                     ESKILAASFKNVDQVNQAFANGAQAITAGADIFEAAFSMPSIEKAVNDFADDWSAIHG
                     RYTI"
     gene            12425..13381
                     /locus_tag="AC1PIW_000972"
     CDS             12425..13381
                     /locus_tag="AC1PIW_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIP6605921.1"
                     /translation="MKQFSGLSRTIQGNHLLDTCAIDILKIGHRPLVLCAKQEYNTTV
                     ESFVKVLTCHGLIVKIAYMEEWDQNSVEEYGEVGFRHRADHIIGIGNSSVAACTKAVA
                     DLLDLPAVLIPTSQFTTLPWVSVENKKRERPLFNESIQLIIADREQLLTQPTSELING
                     VVNTLLYLAAIKEVQRNHLFSLIDDILIRDFESVLLRSSLQAIENFEARILSKEVQDV
                     LEVLSTCVTLDLPSDAWDVIQQIESKLSERREDLVDEPLKRNVVLYLLLTYLTQESEE
                     FQARLKWSQQLLLSANSSNLLSLEAVFKEIALEMGEDGSTRC"
     gene            13502..14596
                     /locus_tag="AC1PIW_000973"
     CDS             13502..14596
                     /locus_tag="AC1PIW_000973"
                     /EC_number="1.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008888 - glycerol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HIP6605922.1"
                     /translation="MKVFASPSRYIQGKHVLFQGAEAIGKLGTKPLILCDDLVYGIIG
                     EKFLSYLVEEGMQVHRVAFNGEASDKEIQRVVEIGKEQASDVVIGLGGGKTIDSAKAI
                     ADLLGVPVVIAPTIASTDAPTSALSVIYSEEGAFERYIFYKKNPDLVLVDTAIICQAP
                     PRLLASGIADGLATWVEARAILQSNGTTMAGGGQTLAGIAIAQTCEQTLFEYGLQAMA
                     SCEAKVVTAALENIVEANTLLSGLGFESAGLAAAHAIHNGFTALEGDIHHLTHGEKVA
                     YGTLTQLFLENRPKEELEKYIRFYQALNLPTTLEELHLADASYEELLKVGQQATIEGE
                     TIHGMPFAISAEDVAEALMAVDYYVRSLDK"
BASE COUNT         4441 a         2556 c         3304 g         4387 t
ORIGIN      
        1 gaaaatattg atgaaatctc atttaattct agtgacggta ggggcttcaa ggttagtgag
       61 aaagatataa aaatatttga taaaaaagaa tttctttctt ccgaagagat tcaaatttcg
      121 ggcacaatta cagttttaaa tacacatgat aacactggaa aaatacaaat tgaatcaaat
      181 gaaattccgt tgggtgaata taaattcgat gttcaaaaac agattcgcta ccctgaatac
      241 cttagttcag ctatgagaaa gaaattgaga tatagatgtt atcctaaaat aaatttcgat
      301 ccgtcaactt tgaaagagaa agttattggt gtgcggatta ttgaaaaatt gtagttcaag
      361 aacattcaat actttcagaa aataccccct aatcatgttg actagggggt atttttctaa
      421 gtcgtttcta tctcttttcc ttgcggtaag gttgttacaa tgtttattgt agcggagtta
      481 gtttctgttt gatttttctg tcgcgttctt tctgaaaaat cttggcgaat atcatttagg
      541 acttgcaaga taactttttc aggggctgtt gggtcatagc tgattttatt gaccaagtga
      601 ctataaagga gggtgtacag attgaaggct gtttttcgga cttccaagct gtttattttt
      661 tcgcgagtgc tggtgtcctg acttcttttc agatagagac gatatgtact ttcatggctt
      721 tcacggagat tggcaatagc ttcatctaac ataagtaggc taacatcttc tgagtagggt
      781 ggcttcgcca gtcgggctag caaagaatca atcaaagcag atgattcttg catattggta
      841 gaccttgtat ttttgtatag tttgatgagt tcttgtagtc ggtgataggc ttgtaatttc
      901 tcgggtgtcc gagtattttt aaaaagtttg agtccgttga cgaaggcaga gtagtcagcc
      961 tttcgattct ttagagcctt gtttaagtta ctagtcatgt ctgagccacg tttttgtttg
     1021 agactttttt tcagtaaggg taaggattct tcaagctgtt tgattaagtc cgttaccatg
     1081 gggtcattgg tggaagaact gtactgtttc aaggtatcaa cactacttgt gatcaattgt
     1141 gtaaattctg cttgattcag atgttgtgca tataaaggga ttcggatttt catttgtgta
     1201 cctcactatc tttttttccg gtcatcttat ttattcgtaa ataagtagaa aaaaatagaa
     1261 ttatttaaaa aattttttaa aacttgtcaa aacagtacat ttacctataa taaaattgtg
     1321 tttttaagga attgcatcaa aacacaatga tataaatgtt tggaaatagc aaattagttt
     1381 gacttgcata tttaaaaatt gtttttggaa taaaattatt gattaggctt gttttcaaat
     1441 tgaaaggata tgatctcagt ttgtttattg aataccttgc acacattttt ctttgtttag
     1501 gcaattccgt ggtacaataa ggtatcaacc attggaggtt ttatatgaaa tggaataaaa
     1561 aactagcact tagtgctgtc ttagtcgcaa gtttgtttac tttgtcagcc tgtcagtcga
     1621 tttcaaattg gtggaaaaat accaaggaag aatggattgg cttggagatg acggtacgga
     1681 cttatgatga aaattctcag ttgatagatg aaatgtctgg taagtcccta tcgatttcgc
     1741 ggaatcagga atttgactcg gtggatgccg aaggctattc caatgcggat tcttctgtct
     1801 tgaaggtgac acttgggaat tacgaaattg accacgtagg ctcatctttg attgcggcag
     1861 aagaaggttt ggaagacttg tatgcgaaat atcagaccac agtagatatc gcaaattacg
     1921 atcgtgcaat tccgcttgtt aaccgtatgg tatctagtct caaaaatgat tttacaggta
     1981 aggcaaaggt ggtcttgatt cgttcgcaaa acggaacccc gcttgctact tatgtaggtg
     2041 ataaagtatc tctttatgca tctgatgcac ctaagacttc tgaactctta attgatggca
     2101 agcggttgat tatctaccgc tgtgattata caatttatga tagagaatta ttggagaact
     2161 aaatgataga cattcgttcc gcaaggatag aggatgcggc agaccttgtg gcaatttatg
     2221 ctccttatgt agaaaagacc gctattacct ttgaaacaga agtaccgact gtcgaagcct
     2281 ttgcaagtcg aattgaaaag accttggaga agtttcccta tctggtcgca gtagaagaag
     2341 gtaaagttgt gggttatgcc tacgcttcaa cttactatgc ccgtgcggct tacgattgga
     2401 cggtggaatt atccgtatat atcaagcaag aagcgcgcgg aaaaggaatt ggaactcttc
     2461 tctacaatgc tttggaaaag gacctgacag cgcgtggttt taaaaatttc ttagcctgta
     2521 tcgccctacc aaacgcagcc tctatagcac tccatgagaa gaggggctat gaacaggtgg
     2581 ctcatttcaa aaaagttggt tataaatttg atacctggca tgatattgtc tggcttcaaa
     2641 aatttcttgt aggtaagcaa aatgaagatt aacgaattag aaaaacgttt gttggccgtg
     2701 gcggatgcta gtcaggccca gcctatgaaa gcttatatga aaaataactt tgaattctta
     2761 ggtgtacgaa cgcctgaccg ccgaaaagtt gcgaagcagt ttttcaaaga tttcaaggct
     2821 cagggaattg actgggactt tgtagaggct tgctggtcta agccctaccg tgaatttcaa
     2881 tatatcgcta ttgattacct agttaccaag aagaaagact tggttctagc tgatttacct
     2941 cgtttgaaag agctagctca gaccaagtct tgatgggata gtattgacgg tctagataag
     3001 ctagttggta agattatctt ggaaaatcca gaggccaagc agacaatctt ggaatggagt
     3061 ttggataatg atttctggtt gagacggatt gccattgacc accagcttct ccaaaaagaa
     3121 aagacagata cgaaactgtt ggaacaaatc ctaatcaata atctcaatca gactgaattt
     3181 tttatcaata aggcaattgg ctggagtttg cgggattatt ccaagaccaa tcctgaatgg
     3241 gtacgagttt ttcttaccaa gtacagctca caaatggcag gcttgtccat tcgcgaagcc
     3301 agtaagtata tctaggagga gccatgtcca aagtctatga atttgaaacc cttatccatc
     3361 cagtaccgga taagggcgga gcctatatca tctttcccta tgatatacga gaagagtttg
     3421 gtaaggggag ggtaaaggta catgccagat ttgattgtca tccctatgat ggctccattg
     3481 tcaatatggg agttaaggat gaagcaggca atatctgtta tattattggc gttcaaaagg
     3541 ctattcgagc ggctattgga aaacaggctg gtgatagagt acaggtaacc attcaagaac
     3601 gaatggagta atcaggcttg caaatccttg agattatggt agaatagaat catgcgatga
     3661 gccgagtagt ggggggatta aggttcagca cacttctaag agttcgtgtc aacatctcag
     3721 cgcagtggtt gattggcaga tttgttcgta ttttatactc caaatctgac ctaatcaact
     3781 gtgcgggggt gggaagacga actcttggtt gacaagttga gttctttccc actcccttcg
     3841 acgctaggga ggtcttcatt taattgaaac gcaggagcgg accttgaata gttgtgtgaa
     3901 ccttctattc tcatcggaaa cgatgccctt gcccaccttt atggtgggct ttttgtatac
     3961 taagtgaggc tgggcaaaaa gccctggccc acttctcagt gttcgtgtca acatctcagc
     4021 gcagtggttg attgggttta acagtccagt ggactgttaa aggtaggaga taagatttgc
     4081 gaagcaaatc tcagcaattc gtgttttaca ctcgaaatct aacctaatca actgtgcggg
     4141 ggtgggagtg aaacagtctg gggatagact gtttcagctc aacaacttaa aacgaaaaat
     4201 tgttgacgaa ccttttttga cttagtcgat gtctttccca ctcccatttc agtatgtgat
     4261 ttctatagga atgctattgg ctcagccgag gaagttttta tatttctcaa agttagaaat
     4321 aacagttgat attatgtttg atgaagattg cttgatagtc ttttaaatct tctggatgga
     4381 gagccttgac atcggtcata gcataggttc gattgagaag cttatactta ttttctccaa
     4441 attgatggaa ggggagtagt tgaactttgt caatcttgag ttgattaaag agacaggcga
     4501 attgttcagc atcttcgagg gaattattaa agtcagggat aacagggata cgtaggacga
     4561 tttctttccc gatggaaaag gcgtattgga tattttgtat gataaggtta ttattgacgc
     4621 cggtgacttt tctatgagaa attgtattgt aatgttttaa atcagtatag ataaagtcaa
     4681 cgtattggat aaggtcaaca aatttgtggt gttctacgaa agcggtggtt tcaatagcgg
     4741 tatgaaggcc gtgtcttttt gcctccataa gaatagcttt cgcaaactca tattgggcaa
     4801 aaatttctcc gccagacagc gtgagcccac cacctgattc ttcataaaaa tcaatatctt
     4861 tcatgacttc ggatatgatg tcatcaacag atttttcttc tcccatgatg gtcatttttt
     4921 tactaacagc atcaagcatg ggttcaggtt ttcgtttttg agattctgga tttgcacacc
     4981 aaggacaacg tagggggcaa cctttcaaga aaaccgtcgt acgtatacca ggaccgtcat
     5041 ggagcgaaaa atgttgaatg ttaaaaataa tgccttttcg tgtattcatg ctctttctcc
     5101 ttttcgatta atttgatttt atcacacaaa agaaagtttt acaattacga acgtaagtta
     5161 aaaagttgca aatagttgaa aaagagtcaa aatttgatag aataatgatg aggtgatgaa
     5221 atggaacgat tagataaaat tatacaactg gtatcacaac atgaaaaaat agatgtcaat
     5281 agcttggcgg agcaactgga cgtttctaag gtaaccatcc gtaaagattt agataagtta
     5341 gaatcaaaag gtttattacg tagagaacat gggtatgctg ttctaaatag tggtgatgac
     5401 cttaatattc ggatgtcctt tcgctatgat gtaaaaaaac gaattgcaaa agaagcagca
     5461 aatctcattg ccgacaatga aacgattatg atagagtctg ggtcaacttg tgctctcttg
     5521 gcagaagaaa tttgcaaaac caagagaaat gtgaccatca taacaaattc ttattttata
     5581 gccaattatg tgcgacaata tgatagctgt cagattattt tattgggtgg tgagtttcaa
     5641 aaagattccc aagtaactgt tgggccgctc ttgcataaaa tgattcagtt tttccatgtt
     5701 gataaggctt ttgtaggaac tgacggctat gatagtgagc atggttttac aggtaaaaac
     5761 ttaatgcgaa gcgaagtagt acagtacatg tctgacgttg ctgaaaacat gattgtgcta
     5821 acagattcta gtaaatttac aaaacgaggg acagtacgca gatttggttt aagtcaagtt
     5881 gctcaggtca ttacagatac gtcgatttct gaagatcttg tcaaggaatt agaaaatgct
     5941 agagtcacag taaggctagt ttaggttggg ggcagtatga agcaagaaaa aagaaggcag
     6001 ttggcaaaaa tagcctatct ttactacgaa gaggggaaaa gccaagctga gattgctgct
     6061 gatacaggaa tttatagaac gacagtcagt agaatgttga ccaaggctaa gtcagaaggc
     6121 attgtaaaaa tagaaattca agattttgat aggcgcctat atcgactaga aaaatatgtt
     6181 caagaaaaat atggattaaa aggtttagaa ctggttgaaa acagcaccga tgaggaccag
     6241 cttgatttag aaaatcgtct ggcacaggct gcagccgata tgcttaccaa tctaattgat
     6301 gatggaaaga aagttggttt ttcatggggg agaagcctca gtcttattgt agataagata
     6361 gggaatcatc gtatgaaagg tgtcaaattt gttccaatag ctggcggtcc aagtcatatc
     6421 cacgcacgtt atcatgtgaa tactctgatt tacgatatgg ccaataaatt tcgtggagaa
     6481 tgtagtttta tcaatgcgac gattatccag gagaatcaag aacttgctca aggtattctt
     6541 tcttctaaat atttcgagga gctacgtgct gactggaagg acttagatgt agcagcaata
     6601 ggtattggcg gtcgagtcga tgaaaaaaat cgtcaatggt tagatatgtt gacgacggct
     6661 gattttcaag ccttgtcaga tgaaggtgca gtcggtgaaa cctgctgtcg tggtctgaat
     6721 caatacggtc aagctgtcgt ggaggatttg caaaagagga ccattgccat ctctttagat
     6781 gatttaaaag aagtcccaga tacaatagct cttgcatatg gggaggaaaa ggctcaagcc
     6841 attttagctg tattgcgagc tggctacatt catcatctgg taacagatga acgaaccatc
     6901 gtaaaagttt tagaactgga taatgatgtg catgtccata caaatttgta agagagtact
     6961 tcaatcactt aaaggtggtt gaagtatttt ttatcccaaa aaatgcacaa aagtgcagaa
     7021 ataaattaaa acagaaaatg ttctataaat atatttacgc tataaagaga aaatgatatg
     7081 atttacttac gaaagaaata taataactta caaataaaac aaatcggagg aaaaagaaat
     7141 ggaaatgatt gttgcagatc aattgatcat gggattgatt atttatgctg gagatgcgaa
     7201 aagtcacgcc taccaagctt tgtcgcttgt caaagcaggt gaagaagagc gagcagaaga
     7261 ggaattagaa ttggcagatg cagcattatt agaagctcac aatatgcaga caaaattctt
     7321 agcacaggaa gctggtggag caaaatctga tattacagca ctatttgtcc attcgcaaga
     7381 tcatttgatg acgtctatta cagagatcaa tttgattaaa gaaatcattg atttgcggaa
     7441 agaactaaaa aaagttggct aaaacaaaaa aattatattt aggaggaaac cgaaatggtt
     7501 aaaatcgctc tattttgtgc cgctggtttt tctacaggca tgttggtaaa caacatgaag
     7561 attgcagcag cagacgcagg tgtcgaagta gaaatcgacg cgcattccca agggaaactt
     7621 gcagattacc ttgacgaaat cgatgtagct ttactgggac cacaagtagc atatacgctt
     7681 gataagtcaa aagcgctctg tgatgaaaag caagtcccta ttgccgtgat tccaatggca
     7741 gactacggga tgttggacgg gaaaaaagtt cttcaattgg cactcagcat ggtttcataa
     7801 aggagtatgg tcatgtccaa aattgataca caaaaaatta ttgtcccaat catgaaattt
     7861 gtgaatatga aagggatcat cgctctaaaa gatggtatgt tggctatctt acctttaaca
     7921 gtagttggta gtattttctt aattttgggg cagttgcctt ttgaaggctt aaatgccgca
     7981 atagcttcta tatttggagc ggaatggact gaaccgttta tgcaagtcta tagcggaacc
     8041 tttgcgatta tgggcttgat ttcttgtttt gcgatcggct attcttatgc caaaaatagt
     8101 ggagttgagc ctctccctgc gggtgtactg tccttgtctt cattctttat cctcttgaaa
     8161 tcatcttata ttccagttga tggagaacca atcggtgatg ccatcgcaaa agtatggttt
     8221 ggtggtcaag gaattatcgg tgcgattatt attggtttgg tagtgggcgc tatctatacg
     8281 acttttattc agcgtcatat tgttatcaag atgcctgaac aagtaccgca agctattgcc
     8341 aagcagtttg aagctatgat tccagctttt gtcatctttt tgctatctat gattgtctat
     8401 attgtttcca agatggcgac aaacggtggt acattcattg aaatgattta cgatgtgatt
     8461 caagtgccac tacaaggttt gactggttca ttaccaggtg cgattggaat tgctttcttc
     8521 atttccttcc tctggtggtt tggtgtacat ggccagtcag ttgtcaatgg tgtcgttact
     8581 gctttactat tgtcaaacct cgatgccaat aaggcccttt tagcagcaga taaattgtct
     8641 ctggaaaatg gcgcccacat cgtgacccaa caattcttag atagtttctt gattatgtca
     8701 ggttcaggta ttaccttcgg tattgttttt gcaatgctat ttgccgctaa atcaaaacaa
     8761 tataaggctc ttggtaaggt agcggccttt cctgcactct ttaacgtaaa tgaaccagtc
     8821 gtctttggtt ttccaattgt gatgaatcct gtaatgttct taccatttgt tttggtaccg
     8881 atcttagcgg ctttcattgt ctacggtgca atctcaatag gctttatgca accattttca
     8941 ggtgtgaccc taccatggtc taccccagca atcatttctg gttttatggt gggtggttgg
     9001 caaggtgcag ttgttcaaat cattatttta gctgtctcaa ctattgttta tttcccattc
     9061 ttcaaaatcc aagataagat tgcctatgaa agtgaaacga aagaaccagt ttagtcaatg
     9121 atgtcagaca aaagagttgc acaaatgtgc agccggcttc ggaaatctta gcgctcacta
     9181 gcatcaattg acaataaccc ctattacagg atatacttaa tttacaaacg aaacaaaatt
     9241 aagtttcgaa agaaagggta aaactatgaa aacagtgact gaagtagctg gcactagcat
     9301 taaaactgag tattttggaa gtctgactga tcggatgaac aaatacagag aagatgtcct
     9361 tgataagaag ccctatattg acgcggaacg ggctgtttta gcaacaaaag cctatcaaga
     9421 acatagagag aagcccaatg tattaaaaag ggcctatatg ctcaaggaaa tattggagaa
     9481 catgacgctt tatattgaag aagaaagtct gattgcaggt aaccaagctt cttcaaataa
     9541 agatgctccc atcttcccag aatatacctt ggagtttgta ttgaatgaat tggatttatt
     9601 cgaaaaacgg gatggggatg tcttctatat taccgaggaa actaaagagc agttgcgcag
     9661 catcgctcca ttctgggaaa agaataatct tcgttcacgt gcaggtgtct tgcttccaga
     9721 agaagttcaa gtttatatgg aaactggctt ctttggtatg gaaggtaaga tgaactctgg
     9781 cgatgcccac ttggctgtca attaccaaaa ggtattggaa caaggtttgc gtggttttga
     9841 ggagcgagtt cgtgtagcaa aggctaatct cgatttgacg gatcctgcaa gtattgataa
     9901 atatcatttc tatgactcaa tctttatcat cattgatgcg gtgaaagcat acgcagatcg
     9961 ttttgtcgcc ttggctactg aattggcaga gaaagcacca acagctcaac gccgtcaaga
    10021 actactggag attgcacgga tttgtgcgaa ggtgccgtat gaacctgctg aaacatttgc
    10081 agaggcagtg caatcggtct ggtttatcca gtgtatcttg caaatcgagt ccaatggaca
    10141 ttctctatcc tatggacgtt tcgaccaata catgtatccg tatgtgaaag ctgatttaga
    10201 agcaggacgt gaaacggaag acagcatcgt cgaacgctta actaacttgt ggattaagac
    10261 cattactatc aacaaggttc gtagtcaatc acataccttc tcatctgctg gtagtccact
    10321 ataccaaaat gtaaccattg gtggacaaac acgtgataag aaggatgctg tcaatccact
    10381 ttcttactta gtattgaaat ctgttgccca aacacacctg ccacagccaa acttgactgt
    10441 tcgctatcat gcaggcctag atgctcgctt catgaatgaa tgtattgaag tcatgaaact
    10501 tggttttggt atgcctgcct ttaacaacga tgaaattatc attccctcct ttatcgagaa
    10561 aggtgtcttg gaagaagatg cctatgacta cagtgcgatt ggctgtgtag aaacggctgt
    10621 tcctggtaaa tggggatacc gttgtacagg tatgagctat atgaacttcc cgaaagtttt
    10681 attgattacc atgaatgatg gtatcgatcc tgcatcaggc aaacggtttg caccagcctt
    10741 tggtcacttc aaagatatga aatcctttga agtgcttgaa acggcatggg aaaagacact
    10801 tcgtcatcta acacggatga gtgtcatcgt tgaaaatgca attgacattg ctttagaaag
    10861 agaagtacca gacattctct gctcagctct gacagatgac tgtatcggtc gtggtaagca
    10921 cctgaaagaa ggtggagcga tttacgatta catttctggt ctgcaggttg gcattgccaa
    10981 tttatctgat tctctagcag caatcaaaaa attagtcttt gaagaaggac gattgactcc
    11041 agaagaatta tggcatgctt tggaaacaga ctatgctggg gagcgtggta aagaaatcca
    11101 ggaaatgttg attgaagatg caccaaaata tggtaacgat gatgattatg cagaccaatt
    11161 ggtaacagca gcatatgata tttatatcaa tgagattgcc aaatatccaa acactcgctt
    11221 tggtcgtggt ccgattggtg gtatccgcta ttcaggtaca tcatccattt cagccaacgt
    11281 tggacagggg cgtggtactt tggcaactcc agacggtcgc aatgcaggaa caccgttggc
    11341 agaaggttgt tctccatcac ataatatgga taaaaatgga ccgacatccg ttttgaaatc
    11401 agttgccaaa ttaccaacac atgaaattgt tggaggcgtt ctgctcaatc agaaggtcaa
    11461 tccacaaaca cttgccaaag aagaagacaa acaaaaatta attgccctct tgcggacatt
    11521 ctttaaccgc ttgcatggct atcatattca gtacaatgtt gtctctcgag aaaccttgat
    11581 tgatgcacaa ctacatcctg aaaaacaccg tgatttgatt gtccgtgtag caggttattc
    11641 agcattcttc aatgtattgt cgagagcaac acaagacgac atcattggtc gtacagaaca
    11701 tacattgtaa aagaggttat tattatggaa ttcatgctgg acaccttaaa tatagaggaa
    11761 atacgaaaat gggctgaggt tttacccctt gcgggggtga cctcaaaccc aacgattgcg
    11821 aggaaagaag gtgacattga cttttttgaa cgtttgcacc ttatccgtga catcatcggt
    11881 ccaaatgctt cactacatgt gcaagttgtg gcaaaggatt atgaagggat tttggcagac
    11941 gctaaaaaaa ttcgtgaatt ggcaccggaa aatatctata ttaaggttcc ggtaacgcct
    12001 gctggtttgg ctgctatgaa aacattgaaa gcgcaaggtt atcagattac agcaacagcg
    12061 atttataccg tttttcaggg cttgttagct attgaagcgg gtgcagatta cttagcgccg
    12121 tactacaatc gcatggccaa tctgaatatt gattcgaatg cggtcatcgc tcagttatca
    12181 gaagcgattg atagggagtg ttcggaaagt aaaattttag cagcatcatt caaaaatgtg
    12241 gatcaagtga accaagcatt tgcaaatggg gcgcaggcta ttacggcagg tgcagatata
    12301 tttgaagcag ccttttctat gccatccatt gaaaaggcgg taaatgattt tgcagatgat
    12361 tggtctgcca ttcatggaag gtatactatc taaacatttc tatattataa aatagggggg
    12421 cactatgaaa caattctcag gtctttcacg cactatccaa ggaaatcatc ttttagacac
    12481 ttgtgcgatt gacattttga aaataggtca tcgtcccctt gttttatgtg caaaacagga
    12541 gtataataca actgtagaaa gttttgtgaa agttttaact tgtcatggac ttattgttaa
    12601 aatagcctat atggaggagt gggaccagaa ctcggttgaa gaatatgggg aagttggttt
    12661 ccgacatcgt gcagaccata ttattggcat tggtaatagt tctgtggcag cttgtacaaa
    12721 ggctgttgca gatttactag acttgccagc tgttcttata ccgacttcac agtttaccac
    12781 tctaccttgg gtatcggtgg aaaataagaa aagagagcga ccattgttta atgagtccat
    12841 ccagctaatt atagcggata gggagcaatt attgacacaa cctacctcgg agttgataaa
    12901 tggagtggtc aatactcttc tttatctagc agccataaaa gaagtacaaa gaaatcatct
    12961 attcagcttg atagatgata ttcttatcag agattttgaa agcgttttat taagaagtag
    13021 tcttcaagct atagagaatt ttgaagctcg gatattatca aaagaagtac aagatgtctt
    13081 agaagttctt tctacttgtg taacattaga cctgcctagt gatgcttggg acgtgattca
    13141 acaaatagaa tcaaagctca gcgaaagaag agaggatttg gtggatgagc cattgaaacg
    13201 aaatgttgtc ctctacttgc ttctgaccta tttgacacag gaatcagaag aatttcaagc
    13261 cagactgaag tggagtcaac agttgctttt gtctgccaac tctagcaatc tattgtcgct
    13321 ggaggcagtt ttcaaggaaa ttgcactaga gatgggagaa gatggctcga cacgttgtta
    13381 gtaggcatcg ctgtacaatg gcaatattta aaatgcaggc tcatacctct tactttcggc
    13441 aatgctcact gtcgtttatt actcaatata acactatcta ttatttcaag gaggaattct
    13501 tatgaaagta tttgcaagtc catctcgcta tattcaaggt aaacatgtcc ttttccaagg
    13561 tgcagaagct atcggaaaat tgggaacaaa gcctttgatt ctttgtgatg atttagtata
    13621 tgggattatc ggtgagaagt ttttgtccta tctcgttgaa gaagggatgc aagtccatcg
    13681 tgttgccttt aatggagaag catctgacaa ggaaatccag cgcgtcgtag aaatcggaaa
    13741 agaacaggct agtgacgtcg taatcggtct aggtggtggc aagacgattg actcagccaa
    13801 ggcgattgct gatttactag gagtgccagt tgtcattgct ccaactattg cttcaacaga
    13861 tgctccgacc tctgctttat ctgtcattta ttctgaagaa ggggcgtttg aacgctatat
    13921 tttctataag aaaaatccag atcttgtctt ggtagataca gcgattattt gtcaagcacc
    13981 gccacgtctt ttggcttctg gtattgcgga tggcttggcg acgtgggtgg aagcgcgtgc
    14041 tattttacaa agcaatggaa caacaatggc aggaggaggt cagacacttg ctggtatcgc
    14101 tattgcacaa acctgtgaac aaacactctt tgaatacgga cttcaagcca tggccagctg
    14161 tgaagccaag gttgtgacag ctgctttaga gaatattgtt gaagccaata ccttgctaag
    14221 tggtttaggc tttgaaagtg caggtttggc tgcggcgcat gcgatacaca atggattcac
    14281 cgccttggag ggagacattc accatttgac acatggtgaa aaagtggctt atgggacctt
    14341 gacccagctc tttttggaaa accgtcctaa agaagaattg gaaaaataca tccgtttcta
    14401 tcaagccttg aatcttccaa caactttgga ggagttacat ttggctgatg ctagttatga
    14461 agaactattg aaagtcggcc aacaagcgac gattgaagga gaaacaattc acggtatgcc
    14521 atttgctatt tcagcagaag acgttgcaga agctctgatg gcagttgact actatgttcg
    14581 ctcattagat aaataagaag agcattcaca gttgagcatg tccatctaaa ctggtgaata
    14641 gaggttcgaa gtaattgcct tgggaaattc tttctcaggg cttttcat
//
LOCUS       DBNFCK010000040        14612 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.040, whole genome shotgun sequence.
ACCESSION   DBNFCK010000040 DBNFCK010000000
VERSION     DBNFCK010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 14612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14612
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.040"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            39..854
                     /locus_tag="AC1PIW_000974"
     CDS             39..854
                     /locus_tag="AC1PIW_000974"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HIP6605923.1"
                     /translation="MNQQFSLDQFSLKGKIALVTGASYGIGFAIASAYAKAGATIVFN
                     DINQELVDRGMAAYKAAGINAHGYVCDVTDEDGIQAMVAQIESEVGIIDILVNNAGII
                     RRVPMIEMTAAQFRQVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRETV
                     SAYAAAKGGLKMLTKNIASEYGEANIQCNGIGPGYIATPQTAPLRELQKDGSRHPFDQ
                     FIIAKTPAARWGEAEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPEA"
     gene            905..1546
                     /locus_tag="AC1PIW_000975"
     CDS             905..1546
                     /locus_tag="AC1PIW_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HIP6605924.1"
                     /translation="MKIALINENSQAAKNSLIFETLKSVTDAKGYHVFNYGMYGKEGE
                     SQLTYVQNGLLASILLVSKAADFVITGCGTGVGAMLAANSFPGVVCGFAVDPTDAYLF
                     SQINGGNALSLPFAKGFGWGAELNLKLLFERLFAEEMGGGYPRERVIPEQRNARILNE
                     MKAVTYKDLLTVLQEIDQDFLKETISGEHFQEYFFANCQDESIATYLKTVLES"
     gene            1563..2564
                     /locus_tag="AC1PIW_000976"
     CDS             1563..2564
                     /locus_tag="AC1PIW_000976"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HIP6605925.1"
                     /translation="MKKVLLIGEPLIRITPTNYQELTDGVESRVFFGGSEINVACNLQ
                     GFGCETKLLTALPQGSLGNRFLDFLDGKGIDTSSVQRKGERVGVYYMEAGFGCRSSQV
                     YYDRGQSSLEAIQVDHFDMEQLFLEVGLVHFSGITVAIGSAVRAWLVQVLKEAKRRKI
                     PISMDLNWRSKMIGLRDAKKVFSQFATYADYCFGLEPIMANEKDWELFERDTATVHTI
                     RERMQTLKEAYKFQAIFHTIRQTDELGRNLYKAYGLADEFCCSQELKTQVLQRVGSGD
                     AFVAGALYKLLEGTSLKAILDFAVASGTYKCSIEGDHMRQSLRKVESLLEGKNDIIR"
     gene            2575..3216
                     /locus_tag="AC1PIW_000977"
     CDS             2575..3216
                     /locus_tag="AC1PIW_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HIP6605926.1"
                     /translation="MSPSQIISKLKEQGLVVVVRGDDKEVGFKTAQACIAGGITAIEV
                     AYTNPDANMIITELSRCHKDDVSVLIGAGTVLDSVTARQAIMAGASYIVSPSFNIEVA
                     KICHLYAVPYLPGCMTLTEITNALEAGCEMVKLFPGSVLGANYITAIKGPLPHVSIMV
                     TGGVKLNNVSEWFNAGATVLGIGGEFNQLATKGEFGTIQELASSYTSVRDKMK"
     gene            3503..3937
                     /locus_tag="AC1PIW_000978"
     CDS             3503..3937
                     /locus_tag="AC1PIW_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIP6605927.1"
                     /translation="MIKVIVVAHGQFASGILTSLKLIAGEVENIEAIDFSEGMSAQEL
                     KARIKSAILGEREVLILTDLLGGTPFKVSVELATEQKEQNVVVLSGLNLAMILEANFS
                     RLTDDLEQLVGKLITTSKDGIVDSVSLLAEDNHDEELFEDGI"
     gene            3949..5139
                     /locus_tag="AC1PIW_000979"
     CDS             3949..5139
                     /locus_tag="AC1PIW_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HIP6605928.1"
                     /translation="MIKDVLIESIAKKREFRNSTLLSKKEVVAAIELAIQQLVINCEY
                     FGESFPTPATFDNHYPKMDNTEWTNGFWTGALWLGHEYSLNNEMKALAKKNTTSFLER
                     VKQRIELDHHDLGFLYSPSTVADYKLTGSKEALEASLLAADKLMERYQEKGGFIQAWG
                     QLGKAEDYRLIIDCLLNLQLLFFAYEETGEQKYYDVALSHFYASVNTVIRDDASTFHT
                     YYFDPETGKPLKGVTRQGFSDDSSWARGQSWGIYGIPLAYRKIKDPACLNLFKGVTNY
                     FLNRLPEDNVSYWDLIFNDGSGHARDSSATAIAVCGIHEMLKYLPEVDEDKETYKYAM
                     HTMLRSLIDNYANRELAPGRPLLLHGVYSWHSGKGVNEGNTWGDYYYLEALLRFYKDW
                     ELYW"
     gene            5149..5640
                     /locus_tag="AC1PIW_000980"
     CDS             5149..5640
                     /locus_tag="AC1PIW_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIP6605929.1"
                     /translation="MATPNIVMARIDERLIHGQGQLWVKSLGCNTVIVANDKVSEDKI
                     QQTLMKTVIPDSVAMRFFSLQKVIDVIHKANPAQTIFLVIKDLQDALALVKGGVPITE
                     LNIGNIHNAPGKMQVTRSIFFGSEDKAIVRELNDVYKIRFNTKTTPSGNDGAVEVNIL
                     DYI"
     gene            5659..6435
                     /locus_tag="AC1PIW_000981"
     CDS             5659..6435
                     /locus_tag="AC1PIW_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HIP6605930.1"
                     /translation="MTISLIQAALIGLWTAFCFSGLLLGLYTNRCIVLSFGVGIILGD
                     LPTALAMGAISELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVTPEAALALST
                     PIAVGIQFLQTSAYTLHAGAPESAIKHLRAGNLSKFKRVANGTVITFAFLGFALGFLG
                     AYSMDTLLKLVEMIPPVLLTGLSVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLQLPVIGIAIVGSIFALKEFFNKPEQVAVKQEEAHDDWI"
     gene            6422..7237
                     /locus_tag="AC1PIW_000982"
     CDS             6422..7237
                     /locus_tag="AC1PIW_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HIP6605931.1"
                     /translation="MTGSKLTKKDYLNTSLRAFFLQNGFNYSNYQGIGYANVMYPAFK
                     KHFGDDKEGLTKALEENCEFYNTNPHFLPFITSLHLVMLENDLPEEETRGIKMALMGP
                     LAGIGDSLSQFVLAPLLSTIAASLAGQGLVMGPILFFLGMNIILTSIKLLTGLYGYKF
                     GTSVIDKLSDQMATISKVANIIGVTVISGLAVTFVKISVPITFAAGELTEGADQKIVT
                     IQGMLDKIAPALLPALFTILVYYLIKNKKWTTYKLVILTVIIGILGSWLGILA"
     gene            7244..7552
                     /locus_tag="AC1PIW_000983"
     CDS             7244..7552
                     /locus_tag="AC1PIW_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HIP6605932.1"
                     /translation="MEQQTLYYVLIILVALSTILPVLTSYVNRKRQQKALEEQLEKRQ
                     RYLSTLQAGDEVILFSGLHGKIIKLTDSLVELQIAEGVLVYVEKESIMGKAKELLFTK
                     "
     gene            7649..11170
                     /gene="hylA"
                     /locus_tag="AC1PIW_000984"
                     /pseudo
     CDS             7649..11170
                     /gene="hylA"
                     /locus_tag="AC1PIW_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936069.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
     gene            11430..13406
                     /locus_tag="AC1PIW_000985"
     CDS             11430..13406
                     /locus_tag="AC1PIW_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III domain-containing protein"
                     /protein_id="HIP6605933.1"
                     /translation="MNFEEWIWALPSLSSFGEAIEKGLVMQTIFGKIVANQSAESVKT
                     YILNQQDAAYKELLLSCQNLLNDIFIFEDNWDMEPCSVGYHLAPLDWTADCHGDEEWT
                     FMLNRQEYLWKLVLAYLVEKDSRYMEQVKRFIFSWIEQVTDWYPKRTSSRTLDTAIRC
                     LSWLNILPFLIDWGGLSEAERSQLLVSIQKQLSYLHSHYRAKDTLSNWGIFQTGAMLL
                     AHAYIGDQVDLVELHHFARQEIEEQIQTQILEDGTQFEQSFLYHVEVYKLLLGLAFSL
                     PDYRKQWTSILKKMADYVLQMTGPDGKSVALGDSDVHCTSDILQMTAIFFEDGTYLVD
                     NSKLDLGVLFLVGENGLRIFEHLTAQSSSSGGQHFSESGHSIVKEVDSYLCFKAGPMG
                     SAHSHSDQNSLCFYHKGQPIVIDSGRFSYRECEERYLLKSAWAHSTCILDDNAPGQIK
                     GSWEYDTYPQFISHSYKGFEHCHWMQGIYQAQTSQGLPYWHKRQLLMVGGDLLLIVDS
                     VDCSGQHELISQLILDERVKIGEERLNDLRLYSQKDFEIQKTVRSPRYNQLVETNKLV
                     KKESFQDRLVTFTLLADEAIRVKQLPVYQTDYHLIESGLAFSCQGKGLNILLVVSDND
                     ICKGEKLLQVAGYKLRGKCLVVDRDKRQVVRLKN"
     gene            13503..14504
                     /locus_tag="AC1PIW_000986"
     CDS             13503..14504
                     /locus_tag="AC1PIW_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIP6605934.1"
                     /translation="MSEKFTIKDIAELAQTSKTTVSFYLNGKYEKMSKETRERIERVI
                     RETDYKPSVVARSLNSKNTKLIGVLIGDITNTFSNQIVKGIEEEAHKSGYQVIIGNSN
                     YNQESEDKYIESMLMLGVDGFIIQPTSHFRKYSRIIEDKKKHMVFFDSQLYEHRTSWI
                     KTNNYDAVYDATQLCVENGYQKFILITADISRLSTRIERATGFMDALLDCKQSYQDLT
                     IADENTDIEEIKCFLQGVVNPQEKTLVFVPNCWALPLVFNAMKELKYDFPQVGLLGFD
                     NTEWTNFSSPTISTIVQPAFEEGCQATRMLINQIEGGNQVGRQQVLDCTVHWKDSTF"
BASE COUNT         4471 a         2562 c         3367 g         4212 t
ORIGIN      
        1 aaacgcttta caaattaaaa tacaagggag ttacgaatat gaatcagcaa ttttcattgg
       61 atcaattttc tctaaaggga aaaattgctt tggtaacagg ggcgtcgtat gggattggct
      121 ttgcgattgc atcggcctat gcaaaagcag gggcgacaat tgtttttaat gatattaatc
      181 aagagttggt tgatagagga atggcagcct ataaggcagc tggtattaat gcgcatggct
      241 atgtttgcga tgtgactgat gaagatggaa tacaggctat ggttgctcaa attgaatcag
      301 aagttggaat tattgatatt ctagtgaata atgctggtat tattcgtagg gttccaatga
      361 ttgaaatgac agcggctcaa tttcgccaag ttattgatat tgacctaaat gctccgttta
      421 ttgtttctaa agcggttatt ccatctatga ttaaaaaggg gcatgggaag attatcaata
      481 tttgttccat gatgagtgag ttaggacgag aaacagtgtc tgcttatgca gcggctaagg
      541 gtgggcttaa gatgttgacg aagaatattg cttcggaata cggtgaagca aatatacaat
      601 gtaacggcat tggtcctggg tatattgcaa ctccacaaac agctccgtta cgtgaactcc
      661 agaaggatgg ttctcgtcat ccatttgatc aatttattat cgcgaagacc cctgcagctc
      721 gttggggtga agctgaggat ttgatgggtc cagcagtctt tttagcaagt gatgctagta
      781 attttgtgaa tgggcacatt ctttatgttg atggaggaat tctcgcttac attggtaaac
      841 agccggaagc gtaataaacg aattaacaaa tttaacaaat tttgatataa gaagggaaaa
      901 aacgatgaaa attgcactta ttaatgaaaa tagtcaggct gctaaaaata gtcttatttt
      961 tgaaacattg aagtcagtaa cagatgctaa agggtatcat gtttttaact atggaatgta
     1021 tggtaaagag ggagaaagtc agttgactta tgtacaaaat ggtctgttgg cttcaatcct
     1081 tctagttagt aaggcagcgg attttgttat tacaggttgt ggcacggggg taggtgccat
     1141 gttggcggct aatagttttc caggggtagt ttgtggcttc gcggtagatc cgacggatgc
     1201 atacctcttt tcacagataa atggaggaaa tgctttgtct ctgccatttg caaagggatt
     1261 cggttgggga gctgaattaa atctcaaatt actttttgag cgactgtttg cagaggaaat
     1321 ggggggcgga tatccccgtg aacgcgttat tccagaacaa cgaaatgctc gtattttgaa
     1381 tgaaatgaaa gcagtcacct ataaagactt actgacagta cttcaggaaa ttgatcaaga
     1441 ttttttgaaa gaaactattt caggtgaaca tttccaagaa tatttctttg ccaactgtca
     1501 ggacgagtca attgcaacat atttaaaaac agtattagaa agttaaagag tggggggcgg
     1561 ttatgaagaa ggtattactg attggtgaac ctttaattcg cattacacca acaaattatc
     1621 aagaattgac agatggtgta gaaagtcgtg tgttttttgg aggttcagaa atcaatgtcg
     1681 cttgtaatct tcaaggattt ggatgtgaaa ccaagctttt gaccgcacta ccacaaggct
     1741 ctctaggtaa tagattttta gatttcttag atgggaaagg gattgatact tccagtgttc
     1801 aacgtaaggg ggaacgtgtg ggggtatact acatggaagc aggatttggt tgccgttcta
     1861 gtcaagtcta ctatgatcgt ggccagtcta gtttggaagc tattcaagtc gatcattttg
     1921 atatggaaca attattttta gaggttgggt tggttcactt tagtggtatc accgtggcta
     1981 ttggttcagc tgttagggca tggcttgttc aagtattaaa agaagcaaag cgtcgtaaaa
     2041 ttccgatttc gatggatctt aattggcgtt ctaagatgat tggattaaga gatgcaaaaa
     2101 aagtgttctc gcaatttgcg acctatgcag actattgttt tggtctggaa cctattatgg
     2161 ctaatgaaaa agattgggaa ttatttgaaa gagatacggc tactgtccat acaataagag
     2221 aacggatgca aactttaaag gaagcctata agttccaagc aattttccat acgattaggc
     2281 agacagatga actaggcaga aatttgtata aggcttacgg attggctgat gaattttgtt
     2341 gctctcaaga attgaaaact caggtgttac aacgtgttgg cagtggtgat gcgtttgttg
     2401 ctggggcatt atataaattg ttagagggga cgtcgttaaa ggctatttta gattttgcag
     2461 ttgctagtgg aacttataaa tgtagcatag aaggggatca catgcggcaa tctcttagaa
     2521 aagttgagtc tttattagag gggaaaaatg acattatacg ataaaggaga aaaaatgagt
     2581 ccatcacaaa ttatttcaaa attgaaggag caagggcttg ttgtagtcgt tagaggagat
     2641 gacaaagaag tcggatttaa gacggcacag gcctgtattg caggaggaat tacagctata
     2701 gaggttgctt atacaaatcc tgacgcgaat atgattatta cagaacttag tcgatgccat
     2761 aaagatgatg tttctgtttt aattggggct ggaacggttc tagattcagt aacggcacgg
     2821 caagccatta tggcaggagc aagttatatt gtttcaccat cttttaatat agaagttgca
     2881 aaaatctgtc atctttatgc agtgccatat cttccgggct gtatgacttt aacggagatt
     2941 acaaatgctt tggaagcagg ctgtgaaatg gttaaactgt ttcctggtag tgttttgggg
     3001 gcaaactaca ttactgcaat caaaggacca ttaccgcatg tttcaatcat ggtaaccggt
     3061 ggagtaaagc ttaataatgt ttcagaatgg tttaacgctg gtgcaactgt tcttggtatc
     3121 ggtggggaat ttaatcaatt agctactaaa ggagagttcg gtacaattca agaattagcg
     3181 tcctcttata caagtgttag agataagatg aagtagttta tttcaaaata aggactgtat
     3241 ttttaacgtt ttcactttct cctgcctcca acggggttga atgtgggaga aagtgagatg
     3301 ttacagggga aattttgaac ggaaacatgg agatatttgg tactcctgtt tatgaaaaga
     3361 gggagctgga gaatattttt taaataaaag tccaacgaaa tataaaacgc ttacattttt
     3421 ttataaaatc tattgacaag tggttgtcaa tagtctacaa tacaaagtgt aggtaaaccg
     3481 gtaaagttaa aaggagagaa ttatgattaa agtgattgtc gttgctcatg gacagtttgc
     3541 tagtgggatt cttacttcac taaagttaat tgcaggagag gtggagaata ttgaggcaat
     3601 tgattttagt gaaggaatgt cggcgcaaga actaaaagca cgcatcaaat cagctatatt
     3661 aggcgaaaga gaagttttga ttttaactga tttactagga ggtacgcctt ttaaagttag
     3721 tgtagaatta gcaacagaac agaaagagca gaatgttgtc gttctttcag gtttaaatct
     3781 tgcgatgatt ttggaagcga atttttcacg gttaacagat gatttagaac aattggttgg
     3841 taaattgatt accacctcta aagatggaat cgttgattcg gtcagtctgt tagcagagga
     3901 taaccatgat gaagagttat ttgaagatgg tatatgaggg agaaatgcat gattaaggat
     3961 gttttgattg agtcaattgc taaaaaaaga gagtttagaa attccactct attgagtaag
     4021 aaagaagttg tggctgcaat agaattggcg attcaacaac ttgttatcaa ttgtgaatat
     4081 tttggtgaat cgttcccgac tccggccaca tttgataacc attatccaaa gatggataat
     4141 acagagtgga caaatggttt ttggacgggc gcgctttggt taggacatga gtattctttg
     4201 aacaatgaaa tgaaagcgtt agcaaaaaag aatacgacat ctttcttgga acgtgtcaaa
     4261 caaagaattg aattagatca tcatgatttg ggatttctgt attcaccatc aacggtagct
     4321 gattataaat tgacaggtag taaagaggct ttagaggctt cgctcttggc tgctgataaa
     4381 ttaatggagc gctatcaaga aaagggaggt tttattcagg cctgggggca acttggtaaa
     4441 gcagaagatt atcggttgat tattgattgt ttgctgaatt tacaattact tttctttgct
     4501 tatgaggaga caggtgagca gaaatattat gatgtagctc ttagtcattt ctacgcttct
     4561 gttaatacag ttattcgaga tgatgcttca acatttcata cctattactt tgaccctgaa
     4621 acaggaaaac cgctcaaagg ggttacccga caaggattta gtgatgactc ttcctgggcg
     4681 cgtggtcaat cttggggtat ttacggtatt ccgttagctt accgtaaaat taaagatccc
     4741 gcatgtttaa atttgtttaa aggagtaacc aactactttc ttaatcgttt gccagaggat
     4801 aacgtttcct attgggattt aatttttaat gatggtagtg ggcacgctcg tgattcttct
     4861 gcgacagcaa tagcggtttg tggtattcac gagatgctta aatatttgcc agaagttgat
     4921 gaggacaagg aaacttataa atatgctatg cacacgatgc tacggtcctt gattgataac
     4981 tatgctaatc gtgagttagc tccaggtcgt ccattgcttt tacatggggt ctactcttgg
     5041 cattcaggaa aaggtgtcaa tgaaggaaat acttggggcg attattatta cttagaagct
     5101 ttgcttcgtt tttataaaga ttgggaatta tattggtagg aggttaaaat ggcaacacca
     5161 aatattgtaa tggctcgtat tgatgagcga ctaattcatg gacagggaca actatgggtt
     5221 aaatccttag gttgtaatac tgtcatcgtg gcaaatgata aggttagtga agataagatt
     5281 cagcagacat taatgaaaac ggttattcca gattcagtag ctatgcgttt cttctctttg
     5341 cagaaagtga ttgatgtgat tcataaggct aatcctgctc aaactatttt cttagttatt
     5401 aaagatttgc aagatgcact agctttggtt aaagggggag taccaatcac agaattaaat
     5461 attggaaata ttcataatgc gcctggaaaa atgcaagtaa cacggtctat cttttttggt
     5521 tcagaagata aggctatagt gagagagttg aacgatgttt ataagattcg cttcaacaca
     5581 aagacaacgc cttctggtaa tgatggggca gttgaagtta atatattgga ttatatttaa
     5641 aaaataagga gaattatcat gacgatcagt ctaattcaag cagctcttat tggtctgtgg
     5701 acagctttct gttttagcgg tctattattg gggctttaca caaaccgatg tatcgtgctt
     5761 tcttttgggg tcggaatcat tttaggagat ctaccaacgg ctttagctat gggtgctatc
     5821 agtgagctgg catacatggg atttggtgtt ggtgctgggg ggaccgttcc tccaaatcca
     5881 atcggacctg gtatttttgg tactttgatg gctattacaa gtgctggaaa agtaacacca
     5941 gaagcagctt tagcactttc aacacctatt gcggtaggta ttcaattctt acaaacttca
     6001 gcatatactt tacatgcggg tgctccagaa tcagctatca aacatcttcg tgcaggaaac
     6061 ctttctaaat ttaaacgtgt tgcaaatggt acagtgatta ctttcgcctt tttaggattt
     6121 gctcttggtt tcttgggtgc ctattcaatg gatactcttt tgaaattggt tgaaatgatt
     6181 ccaccagtat tattaaccgg attgagtgta gcaggtaaga tgcttccagc tatcggtttt
     6241 gcaatgattt tgtctgtaat ggctaagaaa gaattgatcc cgtttgtatt gcttggctat
     6301 gtttgtgcag cttatctaca actgcctgta attggtatcg caattgtcgg tagtatcttt
     6361 gcacttaaag aattctttaa taaaccagaa caagtggcag tgaaacagga ggaagcacac
     6421 gatgactgga tctaaactaa ctaaaaaaga ttatctcaac acttctttac gggccttctt
     6481 cttgcagaat ggtttcaatt acagtaatta tcaagggatt ggctatgcaa atgtcatgta
     6541 tccagctttt aagaagcatt ttggtgacga caaagaagga ttaactaaag ctttagaaga
     6601 aaactgtgag ttttacaata ccaatccaca ttttcttcca ttcattacta gtcttcattt
     6661 ggtcatgtta gaaaatgatc tcccagaaga agagactcgt gggattaaga tggctctgat
     6721 ggggccgttg gcaggaattg gtgactcact ttcacaattt gtattagccc cacttttatc
     6781 tacgattgct gcctctctag caggtcaagg actggttatg ggaccgattc ttttcttcct
     6841 tggaatgaac attatcttga cctctatcaa gttgttgact ggtttgtatg gctataaatt
     6901 tggtaccagc gtgattgata agttgagtga ccaaatggca acaatttcta aggtagccaa
     6961 tatcatcggt gtgacagtta tctctggttt agcagtaacc tttgtaaaaa tttcggtgcc
     7021 aattaccttt gcggcgggtg aattgacaga aggagcagat cagaaaattg tgaccatcca
     7081 gggaatgctc gataagattg cccctgcatt gcttccagcc ttgtttacga ttttggttta
     7141 ctatctcatc aagaacaaga aatggacaac ctacaaacta gttatcctga cagttatcat
     7201 cggtattttg ggtagctggc tgggcatcct tgcataagcg cctatggaac aacaaactct
     7261 ctactatgta ctaattattt tagtagctct atcgaccata cttcctgtac tcacttctta
     7321 tgtcaatcgt aagcgacaac aaaaagcact tgaggagcaa ctggaaaaac gccaacgcta
     7381 cttatcaacc ttacaggctg gagatgaggt tatcctcttc tctgggctgc atgggaaaat
     7441 catcaaacta acagacagct tggtagaatt gcagattgca gaaggtgttc ttgtttacgt
     7501 tgaaaaagag tcgattatgg gaaaagccaa agaactactt tttacgaagt agttcttctt
     7561 tcttgcctcc gcaaggaagc ggttgcagga ttaaaattat acccctggga cattgttttg
     7621 ccccgcataa aaaggaaagg aaacaccaat gggatttttt atcagtcaaa gcaagcagca
     7681 ttacggcatc cggaaatata aggtgggtgt ctgctccgcc cttatcgcgc tgtctatctt
     7741 gggaacaagg gttgcagcca atcaactgcc aagtacagaa acagctagtc cacaaagcag
     7801 tcagttagtg gaaacaactc cagaaacgac agaagcagtt aatcttacga cagaagcagt
     7861 aatgacaagc gaggtcagct ctgaggttag tcctgttaca tcaacagaaa cccagccttc
     7921 aagcaccgct gcagaaactc ttgctagtcc gcaagctgtt caggcgacaa aagaagaaga
     7981 gaagaaccta gtagccaacg gggagttcgc cagtacaaca gcagcatcag gaaattgggc
     8041 agatccggca gctacaaact gggaaacatg gattcctgca aatgtgaaga aagaaaacgg
     8101 acaggttcgc attgatgagg gacggctgca tatctcttca acagctagct atcgagtggc
     8161 agtgcaccaa acagtggatg tagatcctaa caagcgctat ctctttagct ataatgtaga
     8221 aaccaaggac ctgaaaggga gcggtgttcg tgttcgcttg cgtagtttga ctgcagaagg
     8281 taaggacttg agtccacaag aatttgccta tacaccttat aaaaatggaa gtcaagcgga
     8341 gcacatagag cagatcttga cggtatctcc ggaaactcgt aaattgaagg tggaactgtt
     8401 ttttgagaat agtgtcggcc aagcctggct ggataacatc tcgctggtcg aatacgtaga
     8461 aaagacacca gaaacaccag agccaagcct agaacttgtc caacctgaaa cagggcaaat
     8521 aagtcttgcc agcaacaagg tctacctgcc tgttcggcca gacctgacct atcggattgc
     8581 ggatgcggct gtggccatag tagagaaaaa tatgattcgt ccgcttgcgg ctggaaagac
     8641 tcaggtagat gtttatgata aagacaccaa actatctagt ttcgagctga cagttacgga
     8701 gcatcaagca acagtctttg ataccttaag gaacaactgg gaagatattt ctttagccaa
     8761 taaacgctac caatctaacg atactcagat gaaagccttt ctagggcgtt tagatgccgg
     8821 cgtagcttct tctcttaaaa aatgggttga accaacaaat cagggcaaaa cgattttcaa
     8881 cgatattgac tttagtaaat ccagccatct gacaacggtc tatcgccgct tggagcagat
     8941 ggctcaagta gttgagaatc cagactcagc ctattaccac gaccgctcac ttatcgattt
     9001 ggtcagaaaa ggaatgaact ggctttacac caatgtttat aatgaaaata agtcaattga
     9061 tgggaattgg tgggattatg aaattggaac tccgcgtgcg gttgtcaaca ccttgatcta
     9121 catgcatccc tactttagtc aggaggagat tctaacctat accaaaccga tttcaaaatt
     9181 tgtaccagat ccaaccacta tatccgtaaa acattgacca atccagtccc agcagttggc
     9241 ggtaatcaga cggatttgag caaggtggcc atcttggaag gtgctcttcg cgaagatgct
     9301 gaccgtgtga gggctggtgc acaaggtttg acaacgataa tgaaatttgt agataaggga
     9361 gagggcttct acaaggatgg ctcctttatc gaccacacca atgttgccta cacaggggcg
     9421 tatggaaatg tccttattga aggtttttca cagttactac cggttattca gccaacggag
     9481 tttgccctca aggaagaaca gaccaacatt ctttatgaat ggattgaaaa agcctttatg
     9541 ccaatattgg ttcgcggtga actgatggat atgacccgcg gtcgttccat cagtcgggca
     9601 actggtgaat cacatgttca ggctatggag attttaaggt ctttggtccg cattgcagaa
     9661 tcggctcagc ctgagcaaaa aaaacaaact tctctccttt gttaaggcac agctgacctc
     9721 tgataccttc tatgacagct accgcagcct caaatcctat aaggatattg atttggtcaa
     9781 caagctttta gctgataatc aaatccctgc tgaagtagat aaagattata ttgcagcctt
     9841 caacaatatg gataagtttg tttatcgaag cgctcaagaa ggctttacct ttgccttgtc
     9901 tatgtactct tctaaaactc aaaactatga agatatgaac aatgaaaacc gcaagggttg
     9961 gtatacagca gatggcatgg tttacctcta caatgatgat ttgagtcatt acagtaacca
    10021 ctactgggca acagttgacc cgtatcgctt accgggaacg acaacgacca aggacaaacg
    10081 agaagatggc agtggcgagg taactcttgc aagtgacttt gttggagcaa gccagttagg
    10141 caaccgctta gcgacgattg caatgaattt caacaactgg aacaatagtc tgactgctcg
    10201 taaggcttgg attgttctgg gaaataagat tgttttccta ggtactgata tacagcatca
    10261 atctgctcaa ggcgccgtga caacgattga aaatcgtaaa ctcttgaccg gagaaaaata
    10321 tagctattac ataaatggcc agccagttga tttgtccaaa gaagtagtga cagataagac
    10381 tcaatccttt tacatgacca atggcaagga caaccagtcc atcggttacg tcttcttgaa
    10441 ccaactgccg acctatgcca aattagacca gtgaactggc aaatggagtg acatcaacta
    10501 caaccagtcg aaagaagaag tgagcaattc ctttgttagc ctatggcatg aacatgcaca
    10561 gacttcaagt aactatgctt acgtccttgt tccaaaccaa tctatggaaa aagtgaatca
    10621 ggtggctgct agtgtgaaat tgcttcatca agacagggat ttgcaagtgg tatacgacca
    10681 agaacagaat gtctggggcg ttgtgaaata cacggataca gcctacaagc tgacagatga
    10741 tattacctta acagattccg gtttgtatac cattcagaaa gtcgaaggtg gctatcgcat
    10801 cgccttttac aatccgtcta cccgaaccgt aaagaatggc attgagttga caaaggcagg
    10861 ttctagtctg actgttgaga tggagccaac tgcggcttat ccatccactg tctggaaggt
    10921 tactatgcca gaaggttctg ataaacaaac tggaagtgtc gaaaaaacag aaaaagaaga
    10981 aaaacaactc aaggaaaacc aaccaagtcc agaggtgaaa caagtggttc atcatgctgc
    11041 cgaaaaaaca aagtcgagta agccccgtct gccccagaca ggagaagaag caagtttagg
    11101 tcttggtttc ttaggtttgc taaccttggg ggcagtggtg gatttcaagt gtcgcagatc
    11161 gcactcctaa atgaaattcg tgggtgaagg gggagcggga aagaactcga ctagtcaaaa
    11221 aagagttcgt caacaagtct ttatttctag ttgttgagct gaaacagtct atccccagac
    11281 tgtttcactc ccacccccgc atagttgatt agatcagatt cggagtatga aatacgaatt
    11341 gctgaaattt gttttggaac tcctatctcc aacctttagc agtccactga actgttaaag
    11401 ccaatcaatc actgcgctga gatattgata tgaactttga ggagtggatc tgggctttgc
    11461 ccagcctctc ttcttttgga gaagcgatag agaaaggact tgttatgcaa acaatattcg
    11521 gcaaaatagt agccaatcag tcagcagagt cggtcaaaac ctatattctc aatcaacaag
    11581 atgcagctta taaagagctt ctcctttctt gtcaaaattt attaaatgat atttttatct
    11641 ttgaagacaa ttgggatatg gaaccttgtt cagtcgggta tcatcttgct cctcttgact
    11701 ggacagccga ctgccatggg gatgaggaat ggacctttat gctcaatcgg caggaatact
    11761 tgtggaaatt agttttggcc tatctggttg aaaaagattc gcgctacatg gagcaggtta
    11821 aacggtttat ctttagttgg atagaacaag tgacagattg gtatcctaaa agaacgagca
    11881 gtcgcacctt agatacggct atccgctgtc taagctggct caatattctc ccatttttaa
    11941 ttgattgggg cggactaagt gaagctgagc gtagccaact gttagtatcc attcaaaaac
    12001 aattatctta ccttcacagt cattaccgtg ccaaagatac gttgagtaat tggggaattt
    12061 tccagactgg cgcaatgctc ttggcccatg cttatatcgg cgatcaggtg gatttagtag
    12121 agcttcacca ttttgctcgt caggaaatcg aagagcaaat tcaaactcag atattggaag
    12181 atggaactca gtttgagcag tcttttctct accatgtgga agtttataaa ctcttgctag
    12241 gactagcttt cagcttgcca gattatcgaa aacagtggac atctattttg aaaaaaatgg
    12301 cagactatgt cttacagatg acggggccag atggcaaaag tgtggctctt ggcgatagcg
    12361 atgttcattg tacttctgac atcttacaga tgactgcgat tttctttgaa gatggcacct
    12421 acctagtaga taattccaag ctagatttag gcgttctctt cttggtcgga gaaaatggct
    12481 tgcgcatatt tgaacacttg accgctcaat ccagctcgtc agggggacag catttttctg
    12541 aatccggtca cagtattgtc aaagaagtgg acagctattt atgtttcaag gccggtccca
    12601 tgggaagtgc ccacagccac tctgaccaaa atagcttgtg cttttaccac aaaggacaac
    12661 caatcgtaat tgacagcggt cgtttcagct accgtgaatg cgaggagcgc tacttgctta
    12721 aaagcgcttg ggctcacagc acttgtatcc ttgatgacaa tgctccaggg cagataaagg
    12781 gttcatggga atatgacacg tatcctcagt ttattagtca cagttataag ggttttgaac
    12841 actgtcattg gatgcagggc atttatcagg ctcagaccag ccaaggcttg ccttactggc
    12901 ataaacgaca gctccttatg gttggaggcg atttattgct gatagtggat agcgttgatt
    12961 gctcaggcca gcatgagttg atcagtcaat tgattttaga tgaaagagtc aagattggag
    13021 aagagcggtt aaatgatctt cggctatata gtcaaaagga ttttgaaatc cagaaaaccg
    13081 tgcgttctcc tcgatacaac caactagtag agaccaataa actggtcaaa aaagagagtt
    13141 ttcaagatag attagttaca tttactctat tagcagatga agcaattcgt gtcaagcaat
    13201 tgccagttta ccaaacagac tatcatttga tagaaagcgg tttggctttt agctgtcaag
    13261 gaaagggact gaatattctg ttagtggttt ctgataatga tatatgtaag ggagagaaat
    13321 tgcttcaagt ggctggttat aagttgagag gtaagtgctt agtagtggat agggataaga
    13381 gacaagttgt ccgactcaaa aattagaagg gacagaaaaa aatacagtca tttctgtgca
    13441 aaataactga taaagtacgg atagtatgat agaataaagt aactaaagga ggtatcggta
    13501 aggtgtcaga gaagtttacc atcaaagata ttgcagaact ggctcagact tcaaaaacaa
    13561 cagtttcctt ttacttaaat ggtaaatatg aaaagatgtc caaagagaca cgcgaaagaa
    13621 ttgagagagt cattcgcgaa acagactata aacccagcgt tgttgcgcgg agcctaaatt
    13681 caaaaaatac taagttgatt ggagtattga ttggtgatat tacaaataca ttttccaatc
    13741 aaattgtcaa aggtatagaa gaagaagcgc ataagagtgg ctatcaagtt atcatcggaa
    13801 acagcaatta caatcaagaa agcgaagata agtatattga gagtatgctg atgttgggtg
    13861 tagatggttt tattattcag ccgacatcgc attttagaaa atattcgcgc attattgagg
    13921 ataagaaaaa gcatatggtt ttctttgata gtcagcttta cgaacaccgt accagttgga
    13981 ttaagacaaa taattatgat gctgtctatg atgcaactca actatgtgta gaaaatggtt
    14041 atcaaaaatt tattcttatt acagctgaca ttagtcgctt aagtacacgg attgaacgag
    14101 ctacagggtt tatggatgct ctgttagatt gtaaacaaag ttatcaagat ttaacaattg
    14161 cagatgaaaa tacggacata gaggaaatta aatgttttct tcaaggggtg gtaaatcctc
    14221 aggaaaagac cctagtattc gttccgaatt gctgggcgtt acctttagtt tttaatgcta
    14281 tgaaagaact taaatatgac tttcctcagg ttggtctttt ggggtttgac aatactgaat
    14341 ggacaaattt ttcatcccct actatctcta ccattgttca acccgccttt gaagaagggt
    14401 gtcaggcaac aaggatgctg atcaatcaga ttgagggcgg aaaccaagtg ggacgacaac
    14461 aggttttaga ttgcacagtt cattggaaag attctacatt ctaagttaag tcaaatttta
    14521 cttcttagga agaggagcag aacgaagtag aatattctga ttgagagctc actgaaaccc
    14581 tccttgaatc gcctaatata tacaaaaaat ag
//
LOCUS       DBNFCK010000041        14136 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.041, whole genome shotgun sequence.
ACCESSION   DBNFCK010000041 DBNFCK010000000
VERSION     DBNFCK010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 14136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14136
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.041"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            100..288
                     /locus_tag="AC1PIW_000987"
     CDS             100..288
                     /locus_tag="AC1PIW_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HIP6605935.1"
                     /translation="MKLPQFLFLAITTILAVYFMNASILTGDFLIAGIYAFIAYRNLH
                     FAYKVTKFIRLVEKQTKK"
     gene            complement(254..1210)
                     /gene="birA"
                     /locus_tag="AC1PIW_000988"
     CDS             complement(254..1210)
                     /gene="birA"
                     /locus_tag="AC1PIW_000988"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HIP6605936.1"
                     /translation="MKTYQKIYLLLKEREDYVSGEDLAQELGISRTSIWKAIRQLETH
                     GLTIEAARNRGYRLAEGDLLLPELISQELQLPVHLNMDSDSTQLDAKQGIESGHTSPA
                     LYLAPYQNKAKGRFGRPFYASKSGGIYMSLRLSPNVPFLEFKPYTILAAAAVVKAIQS
                     LCDLDVQIKWVNDIYLGHKKVAGILTEAISSMENQRVTDVIIGVGINVRIDDFPKELQ
                     QSAGNLFEEQPPFTRNQLITAIWKAFLETDEKELIALYKEKSLVVGQQVSFVENQVEF
                     KGTAIAVTDTGNLVIQLDNGKAKIISSGEISLTSWSASPPNE"
     gene            1275..2309
                     /locus_tag="AC1PIW_000989"
     CDS             1275..2309
                     /locus_tag="AC1PIW_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HIP6605937.1"
                     /translation="MKTLDYIVTLTNTPSPTGFTTDIMNYIKAEVESFGYVASKTAKG
                     GVLVTVPGENDQEHRMVTAHLDTLGAMVRAVKPDGRLKMDLVGGFGYPSIEGENCLIH
                     CAKNGKTFTGTILMHQTSVHVYRDASTAERNQTNMEIRLDEKVTNADETQALGIEVGD
                     FISFDPRTVVTETGFIKSRPLDDKVSAAILLHLLKVYKEEGATLPYTTHFYFSNNEEI
                     GYGANSSIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDGSGPYHYELRQHLIALAE
                     RDGIPYKLDIYPYYGSDASAAMRAGADVKHALLGAGIESSHSYERTHLDSVVATERMV
                     DAYLKSAMVD"
     gene            2311..3048
                     /locus_tag="AC1PIW_000990"
     CDS             2311..3048
                     /locus_tag="AC1PIW_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605938.1"
                     /translation="MNETRFLELFNDLDKVLRKVCGVADGEYADVGSMLTKAKELTRH
                     NPVEANWDKLYVARQLRNLMVHEKRTGLKEVAQPSQELITVLEKVIAQYQEPITIADY
                     LQKTQKIKPVIFEGKDKLTKALEIVEQEHFSKFPIFSDKVYQGLVSDKGLTNWLAKAS
                     KETGSIREKLVEASLEDVLACEEDSIQVIVLPITAHLYQVINHFEGRKRTTVLVSRQA
                     SGKLHSPEDLVGIVTAQDLAEIYGMVD"
     gene            3067..3534
                     /locus_tag="AC1PIW_000991"
     CDS             3067..3534
                     /locus_tag="AC1PIW_000991"
                     /EC_number="3.5.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004132 - dCMP deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006220 - pyrimidine nucleotide metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxycytidylate deaminase"
                     /protein_id="HIP6605939.1"
                     /translation="MSTNRLAWDEYFAAQALLIANRATCKRAKVGAVLVKDNKVIATG
                     YNGSVSGTEHCLDQECLMIDGHCARTLHAEVNAILQGAERGIPKGFTAYVTHFPCLNC
                     SKQLLQVGCKRVVYINEYRMDDYAQYLYKEKGCELVHLPLEVVKQAFADAEFI"
     gene            3933..5123
                     /gene="metK"
                     /locus_tag="AC1PIW_000992"
     CDS             3933..5123
                     /gene="metK"
                     /locus_tag="AC1PIW_000992"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIP6605940.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAEDPDAHVAAE
                     TAVYTGSVHVFGEISTTAYVDINRVVRDTIAEIGYTKGEYGFSAESVGVHPSLVEQSP
                     DIAQGVNEALETRQDASQDPLDLIGAGDQGLMFGFAVDETPELMPLPISLSHKLVRRL
                     AELRKSGEIAYLRPDAKSQVTVEYDEDNQPVRVDTVVISTQHDPEVSQEQIRQDVIER
                     VIKEIIPAHYLDDQTNYFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAHPVSVRIDTFGTSTVA
                     ESKLEAAVRHIFDLRPAGIIQMLDLKRPIYKQTAAYGHMGRTDIDLPWEKLDKVEALK
                     TAVL"
     gene            5167..5769
                     /locus_tag="AC1PIW_000993"
     CDS             5167..5769
                     /locus_tag="AC1PIW_000993"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HIP6605941.1"
                     /translation="MSVFAHPRKESFTHALVDRVAEALLENGAEVVIRDLYEIGFDPV
                     LRGEDTIHIEDGKFVRQATVFPADVQVEMDLIAESDLLVYIFPSWWNGMPAIMKGYVD
                     RVFQHGFAYSFESDEPRKLFSTKKAIFFMPTGQPQNADGSLTPIDQAMKTLTSEWLFN
                     SNGAQVIDHIFYGRVPYLTREELELYLVDAREQIQRIFHK"
     gene            5920..7179
                     /locus_tag="AC1PIW_000994"
     CDS             5920..7179
                     /locus_tag="AC1PIW_000994"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIP6605942.1"
                     /translation="MRKIVINGGKTLKGSVTVSGAKNSVVALIPAIILADGIVTLDGV
                     PAISDVDSLVDIMETMGATVKRDGETLEIDPRGVKDMPMPFGKINSLRASYYFYGSLL
                     GRFGQAVVGLPGGCDLGPRPIDLHLKAFAAMGAETTYEGDYMRLATNGQRIQGAHIFM
                     DVVSVGATINTILAAVKAEGRTVIENAAREPEIIDVATLLNNMGARIRGAGTDIITIE
                     GVDSLKGTRHQVIPDRIEAGTYIALAAAVGEGIRIDNVLYEHLESYIAKLEEMGVRMT
                     ISEDSIFVEKQEKLKAVSVKTSPYPGFATDLQQPITPLLLKAEGTGTIVDTIYEKRVG
                     HVQELANLGAKISTRNNHIAFEGPNQLTGTSVKATDLRAGAAMVIAGLMAQGRTEITN
                     IEFILRGYSNIIDKLTELGADIQLIED"
     gene            7275..7847
                     /locus_tag="AC1PIW_000995"
     CDS             7275..7847
                     /locus_tag="AC1PIW_000995"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6605943.1"
                     /translation="MTLWTRLAKFACFETDRLYLRPFSFEDGESFYEIVSNPENLPFI
                     FPALEDRNIAFSTMVEKFMRSPLGNWALVDKYSERMIGALCFEKVDERQLSAELSYFL
                     KKDYWRRGLMTEVVRTLVYLAFYEFGLRELVIVTHEENVASQMVAKKAGLVQVAQYRG
                     SDRYSHKTRNYLKFSLKRAEFSLEMYEENQ"
     gene            7844..8704
                     /locus_tag="AC1PIW_000996"
     CDS             7844..8704
                     /locus_tag="AC1PIW_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HIP6605944.1"
                     /translation="MITIKSQREIDAMNRAGDVLAGIHIGLREIIKPGVDMWEVEEYV
                     RKTCKEKNVLPLQIGVDGELMDYPYATCCGLNDEVAHAFPRHYKLKEGDLLKVDMVLS
                     EPLDKAVVDVSKLNFNDVKAMKKITQTYRGGCADSCWAYAVGQVSEEVQNLMDVTKEC
                     LYRGIEQAVVGNRIGDIGAAIQEYAEGLGYGVVRDLVGHGVGPTFHEEPMVPHYGTKG
                     RGLRLREGMVLTIEPMINTGTWEIDTDMKTGWAHKTLDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            8752..9675
                     /locus_tag="AC1PIW_000997"
     CDS             8752..9675
                     /locus_tag="AC1PIW_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIP6605945.1"
                     /translation="MKKTKIVEGLKTFCSTFFSFYRSADSDVTSVAVAYYLLISIFPI
                     LLTLANLLPYYPFDVDMILSVVAEFVPDKLYPSVSSFITSVLTKPSSSWLGISILTTL
                     WTLSRSMTILQKAFNKAYGINEHRDFIIGHLIGIFLGIGLQVIILLSITLLTFGQAVF
                     SYINKLVPIEDAWLKGLLSQTQLVGLMALFTALVMLYFFLPNVRIKKVRYVFPGTLFV
                     LLTMTSIGKLFSIYVDNYANKLLDFRIVTAVVFLVFMLWFIFMAQVLIIGAMINATVQ
                     SMQVEEFHARDGDIVSILNRLKARFTAIDKE"
     gene            complement(9722..10159)
                     /locus_tag="AC1PIW_000998"
     CDS             complement(9722..10159)
                     /locus_tag="AC1PIW_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HIP6605946.1"
                     /translation="MKKLFYQLINNEVILYLIAGVAATLVYMVTRMGLFLVIPSILLV
                     TLLANAVAILFAFWTNDRFVFKQAREGWPQRLVKFVSARIATLVMDMALAYLLVQAYP
                     QLIGQFVNHDLTLVNAISTLFSQVLVIVVNYFLSKLFIFKNTK"
     regulatory      10188..10290
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            10288..10803
                     /locus_tag="AC1PIW_000999"
     CDS             10288..10803
                     /locus_tag="AC1PIW_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HIP6605947.1"
                     /translation="MNTSKWTARDMAEIALVAAIYVALTLTPPLNAISFGAVQFRLSE
                     MLNFLAFYNRKYIIAVTIGCMISNLIGFGVIDLFVGGFSTLIFVTLGVILFEKYKKDY
                     LFGGLFNKAFFYFSFFFAATMFTIALELKVLYDLPFFLTWLTTAAGELLSLLVGAVVI
                     DKLSKKINFEK"
     gene            10919..11761
                     /locus_tag="AC1PIW_001000"
     CDS             10919..11761
                     /locus_tag="AC1PIW_001000"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIP6605948.1"
                     /translation="MTRYTFSNGVTVPKIGFGTWQIPDGEVTYGAVSYALEAGYRHID
                     TAQIYGNEASVGRAIADSGLAREDIFLTTKVWNDKIGYEDTLASVEESMQKLQVDYLD
                     LLLIHWPNPKAVRDSIGWKERNAQVWKALEELYRAGKVKVIGVSNFMEHHLEALLETA
                     EIIPMANQIMLAPGTPQSELVAYCKAKGILLEAYSPFGTGSLFQSQEAADLAKEAGCS
                     VAQLALAWSLDKGFLPLPKSTSPENIKANLEIDGLAISPTAVAKLDKLEGVKGRLDPD
                     QAEF"
     gene            11840..12790
                     /locus_tag="AC1PIW_001001"
                     /pseudo
     CDS             11840..12790
                     /locus_tag="AC1PIW_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931050.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
     gene            12777..13211
                     /locus_tag="AC1PIW_001002"
     CDS             12777..13211
                     /locus_tag="AC1PIW_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HIP6605949.1"
                     /translation="MDLNKYVQEVSLQDFGKEFRHVAIWNRRLRSTGGRFFPRDGHLD
                     FNPKHLEEQGLEVFRKIVRHELCHYHLYFEKKGYRHGDRDFKELLAAVDGLRYAPQLE
                     QATKPSLIYCCQSCGQVYQRKRRIDLKKYRCGKCRGKLTLKE"
     gene            13281..14042
                     /locus_tag="AC1PIW_001003"
     CDS             13281..14042
                     /locus_tag="AC1PIW_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605950.1"
                     /translation="MTQKREERLIELYEKGVLTKEEARACFKEMNQEPDFLFVEEKVK
                     LNFTLPSFKVFASSKLKQSYSFEGIESLFLKLSEGRLTLAKSKNNQISVEICYNQDAP
                     EDKLPRLYVEKKGLYFHSSLACRLTINLPQEWMSVLDLELGQADARLDYLPFEDISIR
                     SSRDKKQQDIRLTTCGGYPQHLYVQLAQAPLTLQTGKGQGIRGQIESQSGHVLVNRKK
                     KNSPYQFEKSGNDLLFLKVQTGQGGFYVKGIKDVN"
     gene            14032..>14136
                     /locus_tag="AC1PIW_001004"
     CDS             14032..>14136
                     /locus_tag="AC1PIW_001004"
                     /inference="COORDINATES: protein motif:HMM:NF015957.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HIP6605951.1"
                     /translation="MSIELYKVRQGKIVSGVLAGIAHKLVWESWVTRAI"
BASE COUNT         3929 a         2569 c         3415 g         4223 t
ORIGIN      
        1 attagattgg tgttttcttg tttagaaaag aatcgaaacc tatcaagaaa gcatataaaa
       61 attcagccag ttgactgaat ttttggtata atctatttta tgaaacttcc acaatttctt
      121 tttttagcca ttacaactat tttagctgtt tattttatga atgcatcgat tttgacagga
      181 gatttcttga ttgcaggtat ctacgccttt atcgcctatc ggaatcttca ttttgcttac
      241 aaggtgacta aatttattcg tttggtggag aagcagacca agaagtaagg ctgatttctc
      301 cgctggagat aatttttgct ttgccgttat ctaactggat aaccaggttt cctgtatcag
      361 taacagcgat ggcagtaccc ttaaattcaa cttgattttc tacaaagctc acttgttggc
      421 caacgacaag tgatttttct ttgtaaaggg caattagttc cttctcatcg gtttctaaga
      481 aggctttcca gatggcagta atgagttggt tgcgggtaaa tggtggttgt tcctcgaaaa
      541 gatttcctgc agattgttgt aattccttag ggaaatcgtc tatgcggaca ttgatgccga
      601 caccgataat gacatcggtt acccgttggt tttccatgga agagatagcc tcagtgagga
      661 tgccagcgac ctttttgtgt ccgagataga tgtcattgac ccacttgatt tggacgtcca
      721 agtcgcaaag agattgaatg gccttcacaa cagcagctgc agctaaaatg gtgtagggct
      781 taaattctag aaatggaaca ttgggagaga gacgaagcga catataaata ccacctgact
      841 tggaggcata aaagggtctc ccaaatcgtc ccttggcttt attctgatag ggagctagat
      901 atagggcagg actagtatga cctgattcaa taccttgttt ggcatctagc tgggtggagt
      961 cgctatccat attcagatgg acaggtagtt gaagctcctg agagattaat tcgggtagga
     1021 gtaaatcgcc ttcggctaat ctgtagccac gattacgggc agcctcaata gttaaaccat
     1081 gcgtttctaa ctggcgaatg gccttccaaa tggatgtccg tgaaatgccc aattcctgtg
     1141 ccaaatcttc tccgctgaca taatcctctc tctcttttaa aaggagatat attttctgat
     1201 aggttttcat accttcatta tagcaaaaaa atcacctaaa acgtgatata atagttttaa
     1261 ataaggaggt tttcatgaag acacttgatt acattgttac attgaccaac actccgtcgc
     1321 caactggttt tacgacggat attatgaatt atatcaaggc agaggttgaa agtttcggct
     1381 atgttgctag taagaccgct aaaggtggtg ttttggtgac agttccgggg gaaaatgatc
     1441 aggaacaccg tatggtgact gctcacctgg acacactggg ggccatggtt cgtgcagtga
     1501 aaccggatgg ccgactcaaa atggatttgg tgggaggatt tggataccct tctattgagg
     1561 gagaaaactg cttaattcat tgcgctaaaa atggcaagac tttcacaggt actattctca
     1621 tgcaccagac ctctgtccat gtctatcggg atgccagtac cgctgagcgc aatcagacca
     1681 acatggaaat tcgtttggac gaaaaagtga ccaatgcgga cgagacgcag gccttaggta
     1741 ttgaagtcgg agattttatc tcctttgatc ctcgtaccgt cgtgacagaa actggcttta
     1801 tcaagagtcg tcccttggat gataaggtat ccgcagctat cttgctccat ctattgaagg
     1861 tctataagga agagggagcg accttgcctt atacgacaca tttctatttt tcaaacaatg
     1921 aagagattgg ctacggtgcc aactccagca ttcctgctca ggtggttgaa tatttggcag
     1981 tcgatatggg ggcaatggga gatgaccagc agacggatga gtacacggtt tctatctgtg
     2041 tcaaggacgg atcaggtccc taccattatg aacttcgtca gcacttgata gccttggctg
     2101 aaagagatgg aattccttat aagctggata tttatcctta ctatggttcc gatgcttcgg
     2161 cagctatgcg agcaggtgca gatgtcaagc atgcactgct tggtgctggc attgagtcta
     2221 gccattccta tgaacggacg catttggatt ctgtagttgc gactgagcgg atggtagatg
     2281 cctatttgaa atcagcaatg gtagattaag atgaatgaaa cccgtttttt agagttattc
     2341 aatgacttgg ataaggtctt gcgtaaggtg tgcggtgttg cggatgggga atatgcagat
     2401 gtaggctcta tgttgaccaa agcaaaggaa ttgacgcgac ataatcccgt tgaggcgaac
     2461 tgggataaac tctatgtggc tcgtcagttg cgtaatctca tggttcatga gaaacgaaca
     2521 ggtttgaagg aagtagccca accttcgcag gaattgatca ctgttttgga aaaagtgata
     2581 gcccagtacc aagaaccgat taccattgcc gactatttgc aaaagaccca gaaaattaag
     2641 ccggttattt ttgagggcaa ggacaaattg acaaaggctt tagaaattgt ggagcaagag
     2701 catttttcta agtttcctat tttttctgac aaggtttatc aaggcctggt ttctgacaag
     2761 ggattgacaa attggttggc caaggcaagc aaggaaacgg gctctatacg tgaaaaattg
     2821 gtggaggcta gtcttgaaga cgtactagct tgtgaggaag atagtattca ggtgattgtt
     2881 ctaccaataa cagcccatct ttatcaggta attaatcatt ttgaagggcg taagcgcaca
     2941 accgtcctag tcagtcgtca ggcgagtggg aagcttcatt cgccagagga tcttgttggt
     3001 attgtcacag cacaggactt ggcagagatt tatgggatgg tggattgagt ggagaaagga
     3061 gaaatcatgt caacaaatcg tttagcttgg gatgagtatt ttgcggctca ggcgcttttg
     3121 attgccaatc gggcgacctg taagagagca aaagtcggtg cggttttggt taaggacaat
     3181 aaggttatcg caacaggcta caatggttct gtttcaggaa ccgaacattg tttggatcag
     3241 gaatgcctta tgattgatgg tcattgtgct agaaccttgc atgcggaagt caatgccatt
     3301 ttacaaggag cggagcgggg aattccaaaa ggttttactg cctatgtgac tcattttccc
     3361 tgtctcaact gctctaagca attgttacag gttggttgta agcgggttgt ctatatcaat
     3421 gaataccgca tggatgacta tgctcagtac ttgtataaag aaaagggctg tgagttggtt
     3481 catttgcctc tagaggtggt taaacaggca tttgcagatg ccgaatttat ctaatgattt
     3541 tgtagaagag tggttgcata gaacaaccct ctttattttt aagaaaatgc taggatagtc
     3601 ggtcaatcta tgctatacta gaaacgttat taagtcccga aaaggtagtt tatagactag
     3661 ttaatatttg cagaaacact tgaaacacaa ttaaagaaac tggtaatatt gaatagtaag
     3721 cgtaaaaact ttactacact tcagtcacta ttttcttcaa agctaggcta ctctcatata
     3781 gtctggtatt gtctttctcg ttgggtatct ggaaatttcc ctttgcttaa gacagttgtc
     3841 tttcgcaaag aactagtctt tgactagttc tttgtaacct tagtaacccg ctaggtttct
     3901 agcatatatt ctattttaag ggggacattt ttatgtcaga acgtaagttg tttacgtctg
     3961 aatctgtttc ggagggtcat ccggataaga ttgcagacca gatttcagat gccattttgg
     4021 acgctatttt agcagaggat ccagatgcgc acgtagcggc agagacggct gtttatacag
     4081 gtagtgttca tgtgtttgga gagatttcaa ccactgccta tgtggatatt aaccgtgtgg
     4141 tacgggatac gattgcggag attggctata caaaaggtga gtatggtttt tcagctgagt
     4201 cggttggggt tcatccgtca cttgtagagc aatcgccaga tattgcccaa ggcgtcaatg
     4261 aagccttgga aactcgtcaa gatgctagtc aagatccatt ggatttgatt ggtgcaggtg
     4321 accaggggct catgtttggt tttgcagtag atgaaacgcc tgaacttatg ccgttgccaa
     4381 tttcacttag tcacaaattg gtgcgtcgtt tggccgagtt gagaaaatct ggtgaaattg
     4441 cttatctccg tccagatgct aagtcacagg taacagttga atacgatgaa gataatcaac
     4501 ctgttcgtgt ggatacagtc gttatttcga cccagcatga tccagaagtt agccaggagc
     4561 aaattcgtca ggatgtcatt gaacgtgtga ttaaggaaat cattccagct cattatctgg
     4621 atgaccaaac caactacttt atcaacccaa ctggtcgctt tgtcattggc ggacctcaag
     4681 gggactctgg tttgacaggc cgtaagatta tcgttgatac ctatggtggt tattctcgtc
     4741 atggcggcgg tgccttctct ggtaaggatg cgaccaaagt ggaccgttct gcatcttatg
     4801 cggcacgcta catcgcaaaa aatatcgtcg cagctggttt ggctaaaaag gcggaagttc
     4861 aattggctta cgctatcggt gttgctcacc cagtttcagt tcgcatcgat acctttggta
     4921 cgagcacagt agcggaaagc aagctagaag cagctgttcg tcatattttc gatttgcgcc
     4981 cagctggtat tatccagatg ttggatctta agcgcccgat ttacaagcaa actgcagcct
     5041 atggacacat gggacgtacc gatattgatc tcccatggga gaaattggac aaggtggaag
     5101 ccttgaaaac tgcggttttg taatgaaata gttgtccact tagtgagaag tttatgaaag
     5161 cgttaattgt ctgtctttgc acaccctaga aaagaaagct ttacacatgc tttggttgat
     5221 agagtggcgg aagctctgct agaaaatgga gcagaagttg tcatccgaga tttatacgaa
     5281 ataggattcg atcccgtgtt acgtggagaa gataccattc atattgagga tggtaaattt
     5341 gtacgtcagg caactgtttt tccagcagat gttcaagttg aaatggattt gattgccgaa
     5401 agcgacttac tagtctatat atttccgagt tggtggaatg gaatgcctgc tattatgaaa
     5461 ggttacgtag accgtgtatt tcaacatggc tttgcttaca gttttgaatc tgatgaacca
     5521 cgtaaattat tttccactaa gaaagctatt ttctttatgc caacagggca gccacaaaat
     5581 gcagatggaa gtctgacacc gattgatcaa gcgatgaaaa ccctgacctc agaatggctc
     5641 ttcaatagca acggtgctca agtcattgac catatcttct atgggcgagt accttattta
     5701 acgagagaag agctggagtt gtatctcgtg gatgcaaggg agcaaattca gagaattttt
     5761 cataaatgat gaagagagat tcactaatcg tatataaata tgaaggactg agctcattgg
     5821 ctcggtcctt ttgcatgcta tataactagc tttttccaag actttatgat agaatgaaac
     5881 gtatgccttt ttaggcaaat taagaaagtg aatgttagta tgagaaaaat tgtaattaat
     5941 ggtggaaaaa cactgaaagg gtcggttacc gttagtggtg caaaaaactc agttgttgct
     6001 cttattccgg ccattattct tgcagacggg attgttactc tggacggagt acctgcaatc
     6061 tcggatgtag atagcttagt tgatattatg gaaacaatgg gcgcgacggt caagcgtgat
     6121 ggtgaaacct tggagattga ccctcgtggt gtaaaagaca tgcctatgcc atttggcaaa
     6181 attaatagct tgcgtgcatc ctactatttt tacggttccc tccttggtcg ctttggtcaa
     6241 gctgttgttg gcttgccagg tggttgtgat ttgggaccgc gtccgattga tttgcacttg
     6301 aaagcattcg cggcaatggg agctgagaca acctatgaag gtgactatat gcgcttggca
     6361 actaatggtc agcgtattca aggggcacat atcttcatgg atgttgtcag cgtcggtgcg
     6421 acaattaata ctatcttagc agctgtcaaa gcagaaggcc gtacagttat tgaaaatgcg
     6481 gcgcgtgaac ctgaaattat tgatgtggca actttgctta ataatatggg ggcacgtatc
     6541 cgtggtgcgg gtacagatat tatcaccatt gaaggcgtag atagtctaaa aggaacacgt
     6601 catcaggtga ttccagaccg tattgaagca gggacttata ttgcccttgc tgctgctgtt
     6661 ggtgaaggta ttcgtatcga taatgtcctt tatgaacact tagagagtta catcgctaag
     6721 ttggaagaaa tgggtgttcg catgaccatt tctgaagata gtatttttgt tgaaaagcaa
     6781 gagaaattga aggcggtaag tgtgaaaact tcaccttatc caggttttgc gacagacttg
     6841 cagcaaccca ttactccttt actgcttaag gcagaaggga ctggtacgat tgttgatacg
     6901 atttatgaaa aacgtgttgg tcatgtgcag gagcttgcaa atcttggagc aaaaatctct
     6961 actcgtaaca accatattgc ctttgaaggt ccaaatcaac tgactggtac atctgttaaa
     7021 gctactgatt tgcgtgcggg agccgcaatg gttatcgctg gtttaatggc tcaagggcgt
     7081 acagaaatta caaacattga gtttattcta cgtggttact caaatattat tgataaactg
     7141 acagaattgg gtgcagatat tcaattgatt gaggattaga gagtctaatt gagaactact
     7201 ttctcaaaac gtaatcgatt taaaaaacga aacaatctgg ggctgggagt aattctcagc
     7261 cccttgggta cactatgaca ctttggacaa gactagcgaa gtttgcttgc tttgaaacgg
     7321 accgtttgta tttacgacca ttttcttttg aagatggaga aagtttctat gaaattgtat
     7381 caaatcccga aaatcttcct tttatctttc cagctttgga ggacagaaat attgcttttt
     7441 cgacaatggt tgaaaaattt atgcgttcac cattgggaaa ctgggcctta gttgataagt
     7501 attcggagag gatgattggt gcgctttgct ttgaaaaggt agatgaacgt caactttctg
     7561 cggagctcag ctatttccta aaaaaagact actggagaag aggcttaatg acggaagtag
     7621 tgaggacttt agtctatcta gctttttatg agtttggtct gagggagctg gttattgtca
     7681 cccatgagga aaatgtggcc agtcagatgg tggcgaaaaa agctggtttg gtacaggttg
     7741 cgcagtatcg cggaagtgac cgctatagcc ataaaacaag aaattatcta aaatttagct
     7801 tgaagagggc tgaatttagt ttagaaatgt atgaggagaa tcaatgatta ccattaaatc
     7861 acaacgagaa attgatgcaa tgaaccgtgc tggagatgtt ctggctggta ttcatattgg
     7921 tttgcgagag attatcaagc caggtgtgga catgtgggaa gtagaggaat atgtccgtaa
     7981 aacgtgtaag gaaaagaatg ttttgccact ccaaattggt gttgatggag aattgatgga
     8041 ttatccttat gcgacttgtt gtggattgaa tgacgaggtc gctcatgctt ttccgcgtca
     8101 ttacaaactt aaagaaggtg atttactgaa ggtcgatatg gttttgagcg agcctttgga
     8161 taaagcggta gttgatgttt ctaagctcaa ctttaatgat gtcaaggcta tgaagaaaat
     8221 cacccagacc tatcgtggtg gttgtgcgga ttcctgctgg gcttatgcgg ttggtcaggt
     8281 ctctgaggaa gttcaaaact tgatggacgt gactaaggaa tgtttgtatc gtggtatcga
     8341 gcaagcagtt gttggcaatc gtatcggcga tatcggtgct gccattcaag aatatgcgga
     8401 aggtcttggc tacggtgttg ttcgtgactt ggtagggcac ggcgttggac caaccttcca
     8461 cgaagagccg atggttcctc attatggaac taaaggtcgt ggtcttcgct tgcgtgaagg
     8521 tatggttttg actatcgagc ctatgattaa tacaggtact tgggaaattg atacggatat
     8581 gaagactggt tgggctcata agacacttga tggtggtttg tcttgtcaat acgaacacca
     8641 atttgtcatc accaaagatg gaccggttat tttgaccagt caaggtgaag aagggaccta
     8701 ttaattctcg gtgtggtcgt gaataataga tgaatgagga aggaggagag aatgaagaaa
     8761 accaaaatag ttgaaggttt gaaaacattc tgctcaacct tcttttcttt ttatcgttct
     8821 gctgatagcg atgtaacaag cgttgcagta gcttactatt tgttaatttc tattttccct
     8881 attcttctaa ctctcgcgaa tttactgcca tattatccat ttgatgtgga tatgatttta
     8941 tcggttgtag ctgaatttgt gccagacaag ctctatcctt cggtttcatc ttttatcact
     9001 tctgtattaa caaagccgtc gtcatcgtgg ttagggattt caattctgac aactctttgg
     9061 accctgtcac gtagtatgac tattttgcag aaggccttta ataaggctta tggtatcaat
     9121 gaacaccgtg attttattat tgggcacttg attggcatat ttttaggaat tggtctgcag
     9181 gtaattatcc tcttaagtat tacgcttttg actttcgggc aggcagtttt ttcttatatt
     9241 aataagctgg ttcctattga agacgcctgg ctgaaggggc ttcttagtca aacacagtta
     9301 gtaggtttaa tggctctgtt taccgccttg gttatgcttt atttcttttt gccaaatgtt
     9361 cggataaaaa aagttcgata tgtttttcca ggaacgttat ttgttctgtt gacgatgact
     9421 tctattggaa agttattttc gatttatgtt gataattatg ctaataagtt gctggatttt
     9481 agaatagtaa cagctgtcgt ctttcttgtt tttatgcttt ggttcatttt tatggctcag
     9541 gtactcatca ttggtgcaat gattaatgct acggttcaaa gtatgcaggt agaagagttt
     9601 catgccagag atggggatat tgtttctatc ttgaataggc taaaggcaag atttacagcg
     9661 atagataaag aatgaagtag gaaaatcaag ctatatataa aaatgaggtt ccgttggagc
     9721 ctcatttcgt atttttaaag ataaagagtt tgctcaagaa atagttgacc acaatgacca
     9781 agacttgaga gaagagagtg gaaatggcgt tgacaagtgt caaatcatgg ttgacaaact
     9841 gcccaatcaa ttgtgggtag gcttgtacta ataaataggc tagagccata tccatgacca
     9901 aggtcgcgat gcgggcagat acaaacttga ccaatcgctg aggccaaccc tctctggctt
     9961 gtttgaaaac gaaacggtcg ttggtccaaa aggcaaagag gatagctact gcattggcta
    10021 aaagagtaac caatagaatg gaaggaatga ctaaaaatag ccccattcga gtgaccatat
    10081 agacaagggt tgctgccaca cctgcaatca aatagagaat aacttcgtta ttgatgagtt
    10141 gataaaatag ttttttcata ggactagtct atcattttta gcaaaaatat gctatactaa
    10201 tcaacgttgt tgataaattc aatgaccctt ggggcttgct cactttaccc aagcatattt
    10261 taagcttttt cgctaaagga gaattttatg aatacatcta aatggactgc cagagatatg
    10321 gcagaaattg ctcttgttgc ggcgatttat gtcgctctta ccttgacacc gccattgaat
    10381 gctatttcct tcggggctgt tcagtttcgt ttgtctgaaa tgctcaattt cttggctttt
    10441 tataatcgta agtatattat cgccgtgacc attggctgca tgatttctaa cttgattggt
    10501 tttggtgtga ttgacctctt tgtgggtgga ttctcgacat taatcttcgt tacattggga
    10561 gttatcctgt ttgaaaaata caagaaggac tatctattcg gtggattatt taacaaggct
    10621 ttcttttatt tctcattctt ttttgcggca actatgttta ccattgcttt agaattaaaa
    10681 gtcttatatg atctaccatt ctttctaact tggttgacaa cagcagcagg agaattgctt
    10741 tctctattag ttggagctgt tgttatcgac aaactatcca agaaaataaa ttttgaaaag
    10801 taagtataca ataaatgtga gcagacactt tcgggtgtct tttattttta ggtcgcatta
    10861 ttttgtattg attgacaatt tagtttatac tggggataga agaaatagga ggtcttttat
    10921 gacaagatat acgttttcaa atggtgtaac tgttcctaaa attggttttg gaacttggca
    10981 gatacccgat ggtgaagtga cgtacggtgc ggtttcttat gcattggaag ctggttatcg
    11041 tcacattgac acggcacaga tttatggcaa cgaagctagt gttggtcgag ctatagctga
    11101 tagtggtcta gctcgtgagg acattttttt aacaactaag gtttggaatg acaagattgg
    11161 ctatgaagat acccttgctt cagtagaaga atccatgcaa aaacttcagg tggattatct
    11221 ggatttgtta ctgatccatt ggccaaatcc caaggctgtt cgcgacagca ttggctggaa
    11281 ggaacgcaat gcccaggttt ggaaggcatt agaagagctc tatcgtgctg gaaaagtgaa
    11341 agtaattgga gtttctaact ttatggaaca ccatctagaa gctttgctgg aaacggctga
    11401 gattatcccg atggctaatc agattatgct ggccccagga actcctcagt cagaattagt
    11461 ggcctactgc aaagccaaag ggattctctt ggaggcctat agcccatttg gtacaggaag
    11521 ccttttccaa agccaagaag cagccgattt ggctaaagaa gctggttgta gtgttgccca
    11581 gctggcacta gcttggtctt tagataaagg ttttctccct ctcccgaaat caactagtcc
    11641 agaaaatatt aaggcgaatt tggaaattga tggcttggct attagtccga cagcagttgc
    11701 aaagttggat aaactagaag gagtaaaagg tcgacttgac ccggatcagg ctgaatttta
    11761 attagctatt gagagcatgt agtttatcta cgtgcttttt ctattgagcc atttgtaccg
    11821 ctttcatgct ataatagtag cttctgtcta ctcagcttct gaactggcgc gatatgagtt
    11881 tccagacctc cctgtggaaa aacgctctgc catttccatc gcccgccgtc tgcaagaccc
    11941 tctggctgag ctggtcaaaa ttgatcccaa gtctatcggt gtcggccagt accagcacga
    12001 tgtcaaccag aagtctctgt ctgaaagtct ggattttgtg gtcgatacgg tggtcaacca
    12061 ggtcggtgtc aatgtcaata cggccagtcc tgcccttttg gctcacgttg ctggtcttaa
    12121 caagaccatt tcggagaaca tcgtcaagta ccgcgaggaa aacggtgctc tgacctctcg
    12181 tcagcaactc aaaaaggttc cacgtttggg tgacaaggcc tttgagcagg cagctggttt
    12241 cttgcggatt cctgatgcga ctaacttttt ggacaatact ggcgtgcacc ctgagtctta
    12301 caaggctgtg gaaaatctgt tagaacttct agctattgac cacttagatg aggctgcgca
    12361 ggaaaaattg aagcagcttg ccatcgcaga tacggcagaa aaaatcggtg tcggtcaaga
    12421 aacattgaag gacatcatcg cagacttgct caaacctggt cgtgatttac gggatgactt
    12481 tgaagcacca gtccttcgtc aagatgtctt ggatgtcaag gacctggtag tcggacagga
    12541 attgcaaggt acggtccgca atattgtgga ctttggagcc tttgtggaca tcggtgtcca
    12601 cgaggatggt ctggtccaca tttcacgcat ggtcaaacgc aaacgagata aaaatggacg
    12661 ccaacaagcc ttgccacatc caagcgaagt tctcgcagtt ggggaaatcg taaccgtctg
    12721 ggtggttgaa gtggacatca aacgaaatcg tatcggtctt agccttttga aaccaaatgg
    12781 atctgaataa gtatgtgcaa gaagtctcct tgcaagactt cggtaaggaa tttcggcatg
    12841 tagcaatctg gaacaggcga ttacgctcaa caggtggtcg cttttttcca agggatgggc
    12901 atttggattt caatcccaag catttggagg agcaaggttt agaagtcttt cggaagattg
    12961 tccgccacga gctctgccac taccatctct actttgagaa gaaaggctat cggcatggag
    13021 acagggattt taaagaattg ctagctgcgg tggatggcct gcgctatgct ccccaactgg
    13081 aacaagctac taagcctagt ctgatttact gctgtcagtc ctgtggacaa gtctaccagc
    13141 gcaagcgccg cattgaccta aaaaaatacc gatgtgggaa gtgtcggggt aaacttactt
    13201 tgaaagaata gagaatcagt cttcgggctg atttttttgt ggagaaatgt gatatactag
    13261 agtagaattg aggtgagtct atgacgcaaa agagagaaga acgcttaatt gagttatatg
    13321 aaaaaggtgt cttgaccaag gaagaggcgc gtgcctgttt taaggaaatg aatcaagaac
    13381 cagactttct ttttgtggaa gaaaaagtaa aattgaattt taccttgcca agttttaagg
    13441 tctttgcttc ctccaaattg aaacaatcct atagttttga agggattgaa tcactctttt
    13501 taaaattatc tgagggacgt ctaactttgg ctaagtctaa aaataatcag attagtgtgg
    13561 agatttgcta caatcaagat gcaccagaag ataagcttcc tcgtctttat gttgaaaaaa
    13621 aggggcttta ttttcacagt agcttggcct gtcgtttgac aatcaacttg cctcaggaat
    13681 ggatgtcggt gctggatttg gaacttggtc aggcagatgc tcgcttggat tacttaccat
    13741 ttgaagatat ttctatccgc agttcaaggg ataaaaaaca acaggatatt cgcttgacga
    13801 cttgcggtgg ttatccccaa cacttgtatg tgcaactagc tcaggctccc ttgactttac
    13861 aaactggcaa aggccaggga attcgggggc agattgagtc gcaatcaggt catgtcttgg
    13921 ttaaccggaa aaagaaaaat agtccctatc agtttgaaaa atctggcaat gatttacttt
    13981 tcttgaaggt acaaacaggt caagggggct tttacgtgaa aggaataaaa gatgtcaatt
    14041 gaattatata aagtaaggca ggggaagata gtatctgggg ttcttgccgg catagcgcat
    14101 aaattggtct gggaatcctg ggtaacacgc gcgata
//
LOCUS       DBNFCK010000042        13809 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.042, whole genome shotgun sequence.
ACCESSION   DBNFCK010000042 DBNFCK010000000
VERSION     DBNFCK010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13809
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.042"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            92..1363
                     /locus_tag="AC1PIW_001005"
     CDS             92..1363
                     /locus_tag="AC1PIW_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIP6605952.1"
                     /translation="MKRLIHNKLFLSSFVADLISNFGDTLYYLALMNYVLLLPDTKFA
                     LAMITASETLPILAGLFIGIWADKTKNKLDTILATLVVRVGLYAFVGFLMGFAPALWV
                     VAVVCVINFLSDLAGQYENGLYMPVSLRVVAAEDRETAMAFKMTVKSLLQIAFQASGA
                     ILIGFMSYQNLAFFNAGTFLVSLAIMATLRPALAKLLKENPIQEAEQTETEAGIIKGM
                     RNSLKESYQAVQNIPVLKASIVTIASLNAIFTALSPLVILNMKEFSDFVIVNAGTTVA
                     LISIFFSVGSILGSSIGMALFKNVSLINLLKFSTLMPVLIFSGFFLHNIYVVLTIIFV
                     TAMTLGVFNPKMNALVLNELPEDKLATVGGGIDAFCQIGMVAGQALVALMVTVLSATS
                     ISLIFLLLSVGLVSYTILVGRKSKNEVVVKA"
     gene            1375..2415
                     /locus_tag="AC1PIW_001006"
     CDS             1375..2415
                     /locus_tag="AC1PIW_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIP6605953.1"
                     /translation="MKLDYRDYRSEIVEKFFIPLIVKEREEPIEADFSQKEKGILKDT
                     LDIRDEIEEKLADFRQEVNQVFVWGYIFNILHSLYFYILNDGQDPKTVEEACQLILKL
                     SQEEVEDAMRTMLASENDGHREKTLSLMELLEKTDKKPADKWYWSLAIRNPLETVERS
                     VHLLDKMLPIYQPYFEQARVEREAFARDFDIEKLYRDSKQLSMTGLDALSVENAQFFV
                     LSPWNYWFAYYGNEEFDYMKVALLASCRIDQIMLSNDELDLDDLTTALKVISDSTRYQ
                     VLVELTKPHAKSKDIAERLAITGAAVSFHTQKLINGDLLLFNTKNSDVKYSVNRELLQ
                     EVIAKLTEDFDL"
     gene            complement(2661..3650)
                     /locus_tag="AC1PIW_001007"
     CDS             complement(2661..3650)
                     /locus_tag="AC1PIW_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIP6605954.1"
                     /translation="MQNKTKPLNTSLLSLIQFIGAILVIALHCRRLFETDQLHFIQKS
                     IFSRMVVPYFMVTASFFLRRNYPSLSKQYLIRYSKQYLTWSVLYLPFYLFYLNLQEIP
                     LLHYPLALLVGLTYTGTSYQLWYMPAFLLGLVLVHYSLRKWGWRITACLACLLYLLGS
                     VETYSSYLAESIFLTAFDSYKTFFFTSRNGLFYAPIFLLTGFYLADKLNHPIFQYRQK
                     NKLLVCTALLAFEATVIYLKQGYDKNFLFSLIPFTAYLVAWTLTTDLFRQKKFQFLKE
                     YASYYYFIHVIPVEISFFFLENHSLTIIQQGWLVFFITIITCQLLSWLIINTQ"
     gene            3839..4525
                     /gene="rnc"
                     /locus_tag="AC1PIW_001008"
     CDS             3839..4525
                     /gene="rnc"
                     /locus_tag="AC1PIW_001008"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIP6605955.1"
                     /translation="MKDLHAKLLADFGIDFSDLNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLMISKYLYQKYPDRPEGEMSKLRSTFVREESLAGFSRACGFDRFLRL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKGEKTVEEFIHKVMIPRLEKGNFERVTD
                     YKTALQEILQVNGEIAISYQVIAESGPAHDKTFEVEVSADQRVIGHGRGRSKKLAEQA
                     AAKNAVEARG"
     gene            4528..8061
                     /gene="smc"
                     /locus_tag="AC1PIW_001009"
     CDS             4528..8061
                     /gene="smc"
                     /locus_tag="AC1PIW_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HIP6605956.1"
                     /translation="MYLKSIEMQGFKSFADKTKVVFDRGVTAVVGPNGSGKSNITESL
                     RWALGESSAKSLRGGKMPDVIFSGTESRKALNYASVVVTLDNSTGFIANKQKEIKVER
                     HIYRSGDSEYLIDGQKVRLRDIHDLFMDTGLGRDSFSIISQGRVEAIFNSKPEERRAI
                     FEEAAGVLKYKTRKKETESKLAQAQDNLDRLDDIIYELDNQVKPLEKQAQTAKKFLEL
                     DGQRKELYLNVLVAQLSLGKEKLSEKEAELESVKTELTSYYKQRSELEQENLNLKEKR
                     HRLSEQLEREQAVLLDLTKLISDLERKIEVHKLESSQNESSHQEAQARLENLLTRREQ
                     LAEQIEQKQETLAQLDSSLSSLKDDIAAVDKEISYFSEDPDQVLDHLREQYVALMQEE
                     AEASNSLTKIQQDIANQISLSESKSEDLARLQTEKQTAQEVLDKSRKSLEEADHVLRQ
                     LLESYQTKKTELDQVQTAYQAEQGRLFDLLDQLKGKQARQSSLEAILKNHSNFYAGVK
                     AVLQAAPSLGGIIGAVSEQLTFDTRYQTALEIALGGASQNVIVEDEATAKRAIAFLKE
                     KRQGRATFLPLTTIKPRQLSGQQVSLLESSEGFLGLASDLVTYQPNLDAIFQNLLGTI
                     AIFDSIDHANQAARATKFQVRMVTMDGAEIRPGGAFAGGSNRNNSTTFIKPELDALLG
                     EIAELSSQLQEQESLVAAKKTSLDQTREALETIKAEGEEARLNQQSARIHQEQAENRL
                     AQLSAQYDLQMSQVSPTILTELEEAAAKEEVNVQALNEKKLALDQQINQVRDNRDSIQ
                     ESLQKLQTQKGQLTLEQAELTSQLRFEQTDLRRLQEEKVVADKEISLLEDMIDQKLEA
                     LEDTSIEILEEQLQAASDKQNQTNQILIRLKFELEDIDGQFEDLEERLQQARTKNDDL
                     IRKQAKLEADCEQAGDKLRTLLGNLTEHFKLSFEAAQSQAKEVENLAVAEQSLKDLER
                     AIRALGPVNLDAIEQYDEVNNRLTFLNEQRTDILSARDLLLDTIHEMDDEVKERFKVT
                     FEAIRESFKQTFKQMFGGGSADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKESQFIVVTHRKGT
                     MAAADAMYGVTMQESGVSKIVSVKLKDVEKL"
     gene            8058..8867
                     /locus_tag="AC1PIW_001010"
     CDS             8058..8867
                     /locus_tag="AC1PIW_001010"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIP6605957.1"
                     /translation="MINKIYASDMDGTFLREDHSFDKERFRRILNQFKEKGYLFVAAS
                     GRSMQSLKLVFEDFVDEIGFVAENGSIVEYQGQTIFMDDPISPEIYLPIIAGIDAGPF
                     GSSRSMVLSGLENFYLLKNAEPQFLEAMTNYYAHFRLVDTFEEVREEIIKINAKFSPE
                     EMDDARRWLNDTFEGVTAMTTGFDNIDIIPNGSNKSVGLSHLCAHFGITRQDVVAFGD
                     NQNDLDMFDFAGLALATENAREEVKAQADWMIGHCNDGAVLAYLEEEVNGN"
     gene            8857..9669
                     /locus_tag="AC1PIW_001011"
     CDS             8857..9669
                     /locus_tag="AC1PIW_001011"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIP6605958.1"
                     /translation="MAIKLIALDLDGTLLTSDKRISEANKKALQAARERGVYVVLTTG
                     RPLQAIGTFLEELDLLGENQYSITFNGGLVQENTGRVLDKVGFTIEDVRTIRRVTNDL
                     DLPLDVLYGGDVYSLPSAHESLYLTCNPLLNKIVASDEEMPEDFVYNKAVSAVAADFL
                     DGQIPKIPAELHERFEIFKSRDILLEWSPKGVHKANGLAKLIGHLGIDQSEVMACGDE
                     GNDLSMIEWAGLGVAMANATDEIKSAANLVLPKNNDEDGIAWVIEHYVLNED"
     gene            9671..10945
                     /gene="ftsY"
                     /locus_tag="AC1PIW_001012"
     CDS             9671..10945
                     /gene="ftsY"
                     /locus_tag="AC1PIW_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIP6605959.1"
                     /translation="MGLFDRLFGQKKQEEQVALVEEVEREQSASSEDTVTTEVESATQ
                     EMEKFQSEVAETELTAEQEAQKQRTLDMMAQYYAAKEAAAARVKEAQEQGFDPAIQTK
                     VKEEPVEEVAPVGQESEQDKYQRTLKKTRTGFGARLNEFFANFRSVDEEFFEELEEML
                     ILSDVGVQVASTLTEELRYEAKLQNAKKIDELRRVIIEKLVDIYEKDGQFSEQINFQD
                     DLTVMLFVGVNGVGKTTSIGKLAYKYKQAGKKVMLVAADTFRAGAVAQLVEWGRRVDV
                     PVVTGPEKSDPASVVFDGVKRAVAEGVDILMIDTAGRLQNKENLMAELEKIGRIIKRT
                     LPDAPHETLLALDASTGQNALSQAKEFAKITPLTGLVLTKLDGTAKGGVVLAIRQELD
                     IPVKLIGFGEKIDDIGEFKSEEFMRGLLEGLV"
     gene            complement(10969..11526)
                     /locus_tag="AC1PIW_001013"
     CDS             complement(10969..11526)
                     /locus_tag="AC1PIW_001013"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6605960.1"
                     /translation="MQHKGTQILETERLILRPFQASDVESVFQNWTSDEKVTTYLTWP
                     THQTLQDTEDYVQFCLQSYSQEKTYRWVIELKENQQPIGDISVVSLDERVQAAELGWV
                     LGSKWWGQSYMAEALEAVNHYLLEEVGCLRITAVHDSENRPSGRVMEKVGMTYEGTLR
                     QAARNNRGIVDIAIYSLLHTDRKSR"
     gene            complement(11528..12202)
                     /locus_tag="AC1PIW_001014"
     CDS             complement(11528..12202)
                     /locus_tag="AC1PIW_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HIP6605961.1"
                     /translation="MTEMNIILTQLEVASHAGTLKRYEKIGETKPYYGVPMGAISGIA
                     KAYKNRLDLFAPLWQTGVLEAQYLAIQVAKAKPNQLPQADLENCLNEQISVNVLDKLA
                     SIILSKRKDSKDWEEYLLIQDQAIFQRLGWFLRAKYFAGRTASNQEIEESLDHIRQHL
                     QTADPLVQWTMNQCLVEIAVTYPDYLEQGLAIGQELAVYANMKVPKGCTSAYAPDWIE
                     ALLRRK"
     gene            complement(12402..>13809)
                     /gene="zwf"
                     /locus_tag="AC1PIW_001015"
     CDS             complement(12402..>13809)
                     /gene="zwf"
                     /locus_tag="AC1PIW_001015"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HIP6605962.1"
                     /translation="LYKAGHIRENFAVIGTARRPWTKEFFEQTVIESLGDLPDTPRQA
                     HEFANHFYYQSHDVNDTEHYVALRKLQDDLCEKYNTQHNKVFFLSMAPEFFGTIAKHL
                     KSEQIVDGQGFERLIIEKPFGTSLATATKLNDELAAAFNEDQIYRIDHYLGKEMVQNI
                     FAVRFANIIFEHIWNRDYIDNVQITFAEAIGVEDRGGYYDHSGALKDMVQNHALQVLS
                     LLAMDKPASFKEEDVRAEKIKVFQHLRQPSDEDLKRNFIRGQYAAGSIDGKDYVSYLD
                     EPNIAEGSQTETFAGGVFFVDTDRFRDVPFFFRTGKRLTEKGTRVTITFKHAEDIFGQ
                     PSEANVLTIFIQPTEGFMLSINGKEVGSHFALTPAKLNFRHNATALGNSPEAYEKLFF
                     DVLNGDSTNFSHWEEVKASWSLIDRIVDLWASNQVPLHTYPAGTMGPEAAFDLLESYS
                     CKWVWTPDVWYRERGLLK"
BASE COUNT         3958 a         2553 c         3411 g         3887 t
ORIGIN      
        1 ttaattttta taaaattaaa tattgacttt attgaaataa agtgtaaaat aacattaaac
       61 aaatatttaa ttgatgttta aggagacgtt tatgaaaagg ttgattcaca ataaattatt
      121 tctatctagc tttgtggcag acttgatttc caactttgga gatacgcttt actatctggc
      181 tttgatgaac tatgttttac tcttaccaga taccaagttt gccctggcta tgattaccgc
      241 atctgaaacc ttgccgattt tggcaggcct ctttatcggg atttgggcag acaagaccaa
      301 aaacaaattg gataccattc ttgcaactct ggtggttcgt gtcggtctct atgcctttgt
      361 tggcttcttg atgggatttg caccagccct ctgggttgtg gcggtggtct gcgtcatcaa
      421 cttcctgtct gaccttgctg gtcaatacga aaacggtctc tatatgccag tcagcttgcg
      481 ggttgtggct gctgaagacc gtgaaacagc tatggctttt aagatgacgg tgaaaagtct
      541 tttgcaaatt gccttccaag ctagcggtgc tatcctgatc ggatttatgt cttaccagaa
      601 tctcgccttc ttcaatgcag ggaccttctt ggtcagtctt gctatcatgg caaccctccg
      661 tccagctctt gccaagctac tgaaggaaaa tccaatccaa gaagctgagc aaacagaaac
      721 agaagcaggt attatcaagg gcatgcgtaa cagtctcaaa gaatcctacc aagctgttca
      781 gaatattcca gttctcaaag cgtcaattgt gaccattgcc agcctgaatg ccatttttac
      841 cgcactttca ccattagtta ttctaaatat gaaggagttc tcagactttg tgattgtcaa
      901 tgcaggaaca accgttgctt tgatttccat tttcttctca gttggtagta tcttggggtc
      961 aagtatcggt atggctttgt tcaaaaatgt cagcttaatc aatctcctca aattctctac
     1021 attgatgcca gtcttgattt tctcaggttt ctttctccac aatatttatg tggtattgac
     1081 gattattttt gtgacagcta tgactttggg tgtcttcaat ccgaaaatga atgccttggt
     1141 cttaaatgaa ctgcctgaag ataaattggc gacagtcggt ggagggattg atgctttctg
     1201 ccagattggt atggtagcag gtcaagcttt agttgcccta atggtgacag tgctttctgc
     1261 gacaagtatt tctctcatct tcctcttgct ttctgtagga ctagtaagct atactattct
     1321 tgtggggcgt aagtctaaaa atgaagtagt tgttaaagct tagaaagtat gcgtatgaaa
     1381 ttagattacc gagattatag aagcgagatt gtcgaaaaat tctttattcc tttaattgtc
     1441 aaggagcgtg aggaaccgat tgaggcggat ttttcacaga aagaaaaagg cattctcaaa
     1501 gataccttgg acattcgtga tgagattgaa gaaaagttgg cagattttcg tcaagaagtc
     1561 aatcaagtct ttgtctgggg atatattttt aacattttgc atagtcttta tttctatatc
     1621 ttgaatgatg ggcaggatcc aaagacagtt gaggaagctt gtcaattgat tttaaagctg
     1681 tctcaagaag aagtagaaga tgccatgcga accatgttgg catctgaaaa tgacggacat
     1741 cgtgagaaga ccctcagcct catggaactt ttggaaaaaa cggataaaaa gccggcagat
     1801 aagtggtact ggtcactggc tatccgcaat cccctggaaa cagttgagcg gtctgtccat
     1861 ctcttggata agatgcttcc aatctaccag ccctattttg agcaagcaag agttgagcgg
     1921 gaagcctttg ctagggattt tgacattgag aaactctatc gagactccaa acagttgtcc
     1981 atgactggct tggatgcttt gagtgttgaa aatgctcaat tcttcgttct cagtccgtgg
     2041 aattattggt ttgcttatta tggcaacgaa gaatttgact atatgaaagt cgctctttta
     2101 gcttcctgtc gaattgatca gattatgctg tcaaatgacg agctggatct ggatgatttg
     2161 acaacggctc ttaaagtcat cagcgatagt acgcgctatc aggtcttggt tgagctgacc
     2221 aagcctcatg ccaagagcaa ggacattgca gaacgactgg ctattacagg ggcggcagtg
     2281 tctttccaca ctcaaaagct gattaacggt gacttacttc tcttcaatac aaaaaatagc
     2341 gatgtcaaat acagtgtcaa tcgtgaactt ctccaagagg tcattgccaa attgacagag
     2401 gattttgatt tgtaaaaaaa gccttgtaag ttagatctta caaggttctt ttgtgcttat
     2461 acccttcgaa aatcaaaatc agacgttgtt gacttgattt gatgagtgaa aaggtccagt
     2521 ggatcttttc agcctgttcc cttgaaatat aataaggaac agtgttctat ctgcatctta
     2581 cggtcacgaa ccaagttcgt ctaatttccg acctccaaca gtctattgcc agactgttgg
     2641 agctagtctg tttttgattt tcattgagta ttaatgatta accagctgag cagttggcaa
     2701 gtgatgatgg tgataaagaa aaccagccag ccttgctgta ttatagtcag ggaatgattt
     2761 tctaggaaga aaaagctgat ttcgactggg ataacatgga taaaataata atagctggcg
     2821 tattctttta gaaattggaa ttttttctgg cggaatagat ctgtggttag ggtccaggcg
     2881 actagatagg ctgtgaatgg aatcaggcta aatagaaagt tcttgtcgta gccctgtttg
     2941 agatagatga cagtggcttc aaaagctaaa agggctgtgc agacaagtag tttgtttttt
     3001 tgtcggtatt ggaaaatggg gtgattgagt ttgtcggcta gataaaagcc tgtcaggaga
     3061 aaaatcggtg cgtagaagag gccgttgcgg ctggtgaaaa agaaggtttt gtagctgtca
     3121 aatgcggtca ggaaaatcga ttctgccagg taggaggagt aggtttcgac tgagcctaga
     3181 agatagagaa ggcaggctag acaagctgtg atgcgccagc cccatttcct taaagaatag
     3241 tggacaagga caagacctag caggaaggcg ggcatgtacc agagttggta gctggtgcct
     3301 gtgtaggtca gtccgactag gagtgcgagg gggtagtgga gtagggggat ttcttggagg
     3361 tttaggtaga agaggtagaa gggtaggtag aggacggacc aggtcaggta ctgcttgctg
     3421 tagcggatta ggtattgttt ggagaggctg gggtagttgc gtcggaggaa gaagctggct
     3481 gtgaccatga agtagggaac gaccatgcga ctaaagattg atttttggat gaagtggagc
     3541 tggtctgttt caaataacct gcggcaatgg agggcgataa cgagaatggc tccgatgaac
     3601 tggatgaggg agagcagtga tgtattgagt ggttttgtct tattttgcat gctaacgttc
     3661 ttcttttttc ttctatttta tcgaaatctg atgaatatgt aaatgtttgc aaaaagatgt
     3721 ggagaggtgc tggctgaaac ttagttgaca agagaagtcc agtggaaaat atggtataat
     3781 gttaggacgg agtaaatgta aactatctat ttcgtttaag ttagaaagga gcatatagat
     3841 gaaagattta catgcaaaat tattggctga ttttgggatt gatttttcgg atttgaattt
     3901 attagaaacg gcttttaccc atacttctta tgccaatgag catcgccttc taaaaatttc
     3961 acataatgag cgcttggaat ttttaggaga cgctgttctc cagttgatga tttccaaata
     4021 tctttatcaa aaatatcctg atagacccga aggtgagatg tccaagttgc gttcgacctt
     4081 tgtacgtgag gagtctttgg ctggtttttc acgtgcttgt ggttttgatc ggtttcttcg
     4141 tcttggtaag ggggaagaga agtctggagg gcgaaatcgc gatactattt taggagatgc
     4201 tttcgaggcg ttcttaggag ctctgctatt ggacaaaggg gaaaagaccg tggaggaatt
     4261 tatccataag gtcatgattc ctcgtttaga aaaagggaat tttgagcgcg tgaccgacta
     4321 taagactgct ttgcaggaaa ttttgcaggt caatggtgag attgctattt cgtatcaagt
     4381 aatagctgag tctggacctg cccatgataa gacctttgaa gtggaggtat ctgctgatca
     4441 gcgtgtgatt ggtcatggtc gtggtcgctc taagaaatta gctgaacaag ccgccgccaa
     4501 aaatgctgtg gaggctagag gataggtatg tatctaaaat caattgaaat gcagggcttt
     4561 aagtcctttg cggataagac taaggtagtc tttgatcgtg gagtgactgc tgttgtagga
     4621 ccgaatggtt ctggcaagtc caatattaca gaaagtctac gttgggcctt gggagagtcg
     4681 tctgctaaaa gcctacgtgg aggcaagatg ccagacgtca tcttttcggg aacagaaagt
     4741 cgcaaggctc ttaactatgc ctcggttgtc gtgaccttgg ataatagcac aggttttatc
     4801 gccaataaac agaaagaaat caaggtagaa cgccacattt atcgatctgg tgatagtgag
     4861 tacttgattg atgggcaaaa ggttcgtcta cgggatattc atgatctttt catggatacg
     4921 ggcttgggac gtgattcttt ctctatcatt tcacaagggc gggttgaagc aattttcaat
     4981 tccaagcctg aggaacgccg tgctattttt gaagaggcgg caggggtctt aaaatacaag
     5041 acgcgtaaaa aagaaacaga aagtaagttg gcacaggccc aggacaactt ggaccgcttg
     5101 gacgatatta tctacgaatt agataatcag gtcaaaccat tagagaaaca ggcacaaact
     5161 gctaagaaat ttctagaatt agatggtcaa cgtaaggaat tatacttaaa tgttttggtg
     5221 gctcagttgt cgcttggtaa ggaaaagtta tcggaaaaag aggccgaact agagtctgtc
     5281 aaaactgaat tgactagcta ctacaaacag cgttctgagc tggagcaaga aaatctgaac
     5341 ttgaaagaga agcgccaccg cctttcagaa caattggagc gtgaacaggc agttctgttg
     5401 gatttgacca agttaatcag tgatttggag cgaaaaattg aggtccataa actggaatct
     5461 agtcaaaacg aatcaagtca tcaagaagcg caggcgcgct tggaaaactt gttgactaga
     5521 cgagagcagt tggcagaaca gattgagcaa aagcaagaaa cgcttgcgca attagatagt
     5581 tccttgtcta gcttgaaaga cgatattgct gcggtagaca aagaaatttc ctacttttca
     5641 gaagatcctg atcaggtttt agatcatctg cgtgaacagt atgttgctct gatgcaggaa
     5701 gaggcagaag cttcaaacag tctgaccaag attcagcagg atattgccaa tcaaatcagt
     5761 ctttcagaga gtaaatctga agacttggca cgcttgcaga cggaaaaaca aacagctcaa
     5821 gaagtacttg ataagagtcg gaaaagttta gaagaagctg atcacgtgtt acgccagttg
     5881 cttgagagct atcagaccaa aaaaacagaa ttggatcaag tccagactgc ttatcaggct
     5941 gagcaaggtc gcttgtttga tttgctggat cagttgaaag gcaagcaggc tcgtcagtct
     6001 agtttagaag ctattttaaa aaatcactcc aatttctacg cgggggttaa ggctgtctta
     6061 caggcggcac caagcttagg aggaattatt ggagcagtca gtgaacaatt gacctttgat
     6121 actcgttacc aaacggcttt ggaaattgct cttggcggtg ctagtcaaaa cgtcatcgtt
     6181 gaagatgaag cgactgctaa acgagctatc gcatttttga aagaaaaacg gcaaggacgg
     6241 gcaaccttct tacctctaac aactatcaag ccacgtcagc tttctggaca gcaagtctct
     6301 ttactagaat cttcagaagg attcttgggg cttgcaagtg atttggtgac ttatcagcca
     6361 aatttagatg cgattttcca aaatttgcta ggcactattg cgatttttga tagcattgac
     6421 catgccaacc aggcggcgcg tgcgaccaag tttcaagtgc gtatggtgac catggatgga
     6481 gcggaaatcc gtcctggtgg agcctttgct ggtggtagca atcgaaataa cagcacgacc
     6541 tttatcaaac cagaactgga tgctctttta ggagaaatag cagagctgtc tagccagttg
     6601 caggagcaag aaagcttggt tgcagctaag aaaactagct tagaccaaac cagagaagcc
     6661 ttggaaacga ttaaggcgga gggggaagaa gctagattga accagcagag cgcaaggatt
     6721 catcaggaac aggcagaaaa tagactggct cagctatcag cccagtatga cctgcaaatg
     6781 agtcaggtta gtccgaccat tttgactgag ctagaagagg ctgctgctaa ggaagaagta
     6841 aatgtccaag ccttgaatga gaaaaaacta gccttggatc aacaaatcaa tcaagtccgt
     6901 gataatcgcg acagtattca agaaagcttg cagaaactac agactcagaa gggtcaacta
     6961 actttggaac aagctgaact gactagccaa cttcgttttg aacagacaga tctgagacgt
     7021 ttgcaagaag aaaaagtggt tgcagataag gaaatttctc ttttagagga tatgattgac
     7081 cagaagttag aagctcttga agatactagc attgagattc ttgaagaaca attgcaggcc
     7141 gcatctgata agcaaaacca gaccaatcag attctaattc gcttaaagtt tgagttggaa
     7201 gatattgatg ggcagtttga agacttggaa gaaagattgc aacaagctcg gactaagaat
     7261 gatgatttga tccgtaagca ggctaagtta gaggcggatt gtgagcaagc gggcgataaa
     7321 ttacggactt tacttggtaa tttgaccgag catttcaaat tgagttttga agctgctcag
     7381 tctcaggcca aggaagttga gaatcttgca gttgctgaac aaagcctcaa agacctagaa
     7441 agagctattc gtgctttggg tccagtcaat ttggatgcta ttgagcagta tgacgaggtc
     7501 aataaccgtc tgaccttcct caatgagcaa cggacagata ttttgtcggc gcgtgattta
     7561 ctactagata ctatccacga gatggatgat gaagtcaaag agcgcttcaa ggttactttt
     7621 gaagctattc gtgaaagctt taagcagaca ttcaaacaaa tgtttggcgg tggttcagcg
     7681 gatttgattt tgacagaagg tgacctattg actgcgggtg tggagatttc tgttcaacct
     7741 ccgggtaaga aaattcaatc tctcaatctt atgtcaggtg gcgaaaaagc cctttcggcc
     7801 ctcgctctgc tattttccat tatccgtgtt aagaccattc cttttgtaat tctcgatgag
     7861 gtggaggcag ccttggatga ggcgaatgtc aaacgttttg gcgactacct caaccgtttt
     7921 gataaggaga gtcagtttat cgtcgtgacc caccgtaaag ggaccatggc ggcagcggat
     7981 gccatgtatg gggtgaccat gcaggaatct ggcgtgtcca agattgtttc cgttaagctg
     8041 aaagatgtgg agaaattatg attaacaaaa tttacgccag tgatatggat ggtacctttt
     8101 tacgagaaga ccatagtttt gataaggaac ggtttcgtcg tatactgaat cagttcaaag
     8161 aaaaaggtta tttatttgtg gcagcaagtg ggcgatctat gcaaagcctg aaacttgttt
     8221 ttgaagattt tgtagatgaa atcgggtttg tagctgaaaa tggttcaatc gttgaatatc
     8281 aggggcagac tatctttatg gatgatccaa ttagtcctga aatttatctg ccgattatcg
     8341 caggtattga tgcaggtccc tttggtagca gtcgttccat ggtgctgtct ggcctcgaaa
     8401 acttttattt attaaaaaat gctgagcctc agtttttaga agcgatgacg aattattacg
     8461 cacattttcg attggtagac acatttgaag aagttcgaga agagattata aaaatcaatg
     8521 ccaaattttc tcctgaggaa atggacgatg ctagacgatg gttaaatgat acatttgaag
     8581 gtgtaacggc catgacaact ggttttgaca atattgatat aattccaaat ggttcaaata
     8641 aatcagttgg attgagtcat ctctgtgctc attttggaat tacccgtcag gatgtagtgg
     8701 catttggtga taatcaaaat gatttggata tgtttgactt tgctggtctg gctcttgcga
     8761 cagaaaatgc aagagaggaa gtgaaggccc aggcagattg gatgattggt cattgcaatg
     8821 atggagcagt attagcctat ttagaggagg aagtaaatgg caattaagtt gattgcattg
     8881 gatttagatg gaacacttct gacatcggac aaacggattt cagaagctaa taaaaaggcc
     8941 ttgcaggcag cgcgtgagcg tggagtttat gtagttctga caacagggcg tcctttacaa
     9001 gctatcggta cttttctaga agaactagat ctgttagggg agaatcaata ttcgattaca
     9061 tttaacggtg gtctagtcca ggaaaataca ggtcgagttt tggataaggt tggcttcacc
     9121 attgaagatg tgcgcaccat ccgtcgtgtg acaaatgact tggaccttcc tctggatgtt
     9181 ctctatggtg gcgatgtcta ttctctacca tcagcccatg aatcacttta tctaacttgt
     9241 aatccattgc ttaataaaat tgtggcaagc gatgaggaaa tgccagaaga ttttgtctac
     9301 aataaggcag tttcagctgt ggcagcagac tttttggatg gacaaattcc aaaaattcct
     9361 gcggaacttc acgagcgttt cgagattttc aaatcgcgtg atattctgct cgaatggagt
     9421 ccaaaaggtg ttcataaagc aaatggtttg gctaagttga ttggacatct ggggattgat
     9481 cagtcagaag ttatggcctg tggcgatgaa ggaaatgatc tttcgatgat tgaatgggct
     9541 ggtctgggtg ttgccatggc taatgcgact gatgaaatca agtctgcggc caaccttgtc
     9601 ctgccaaaaa acaatgatga agatggcatt gcttgggtca tcgagcacta tgtattgaat
     9661 gaggactagt atgggattat ttgatcgatt atttggacaa aaaaaacaag aggagcaagt
     9721 cgctcttgtt gaagaagtgg aaagggagca gtcggcttct agcgaagata cagtaactac
     9781 tgaagttgag tcagctactc aagaaatgga aaagttccag tctgaagtag cagagactga
     9841 attgacagcc gaacaagaag ctcagaaaca acggacctta gacatgatgg ctcaatatta
     9901 tgcagccaaa gaagccgctg cggctcgagt gaaggaagcg caggaacaag gctttgaccc
     9961 tgctattcaa accaaggtca aggaagagcc tgttgaggaa gttgctccag tagggcaaga
    10021 aagtgaacag gacaagtacc agcgtacctt gaaaaagacg cgtacaggat ttggggcaag
    10081 gctcaatgaa ttctttgcca atttccgttc ggttgatgaa gaatttttcg aagagttgga
    10141 agaaatgctg attttgtcag acgtaggtgt gcaagttgct tctacgctga cagaggagct
    10201 ccgctatgaa gccaagctac aaaatgccaa gaagatagat gaactccgtc gtgttatcat
    10261 cgaaaaattg gtggatattt acgaaaaaga cggtcaattc agtgagcaaa tcaatttcca
    10321 agatgatttg acagtcatgc tctttgtcgg tgtcaacggt gttgggaaga cgacgtctat
    10381 cggtaagtta gcctacaagt acaagcaagc tggcaagaag gtcatgttgg tcgcagcgga
    10441 caccttccgt gcgggtgcgg tagcccagct ggtcgagtgg ggtcgccgtg tcgatgtgcc
    10501 agtagtcaca ggacctgaaa agtcagaccc agctagtgtg gtttttgatg gggtaaaacg
    10561 tgccgtggca gaaggcgtgg atattctcat gattgataca gcaggtcgct tgcaaaataa
    10621 agaaaacctc atggcagagc tggaaaaaat cggtcgcatc atcaaacgga ccctacctga
    10681 tgcaccgcat gaaaccctct tagcactgga tgcctccaca ggtcaaaacg ccctcagtca
    10741 agccaaggaa tttgcaaaaa ttactccatt gactgggcta gtcttgacca agctagacgg
    10801 tacagccaag ggtggtgtcg ttcttgccat ccgtcaggaa ctggatatac cagttaagct
    10861 aattggtttc ggtgagaaaa tcgatgacat cggtgaattc aaatccgaag aattcatgcg
    10921 tgggctattg gaaggattgg tatagaaaaa agtcgttagt tcaatgaact agcgactttt
    10981 tctatctgta tgcagcagtg agtaaatggc aatatccaca ataccccgat tgtttcgagc
    11041 ggcttggcgg agagttcctt cataggtcat accgactttt tccatgacac gaccagaagg
    11101 acgattttca ctatcgtgaa cagctgtaat ccgtaaacag cccacttcct ccaataaata
    11161 gtgattaaca gcttcaagag cttcggccat ataactttgc ccccaccatt tactgcccaa
    11221 cacccaacct aattcggcag cttgaactct ttcatcaagg ctgacaacgg aaatatctcc
    11281 aatgggttgc tgattttctt tgagctcaat tacccacctg tatgtttttt cctgtgaata
    11341 ggattgaaga cagaactgga catagtcttc agtatcttgg agtgtctgat gcgttggcca
    11401 ggtcagatag gtggtaacct tttcatctga agtccagttt tggaaaactg attcaacatc
    11461 gcttgcttga aaagggcgta aaattagacg ttctgtttct aaaatctgag ttcctttatg
    11521 ttgcatctta tttcctcctc aacaacgctt ctatccagtc tggtgcatag gcagaagtgc
    11581 agccttttgg aactttcatg ttcgcataga cggccaattc ctgaccaatt gcaagacctt
    11641 gttccaggta gtcaggatag gtaactgcaa tctccactaa gcattggttc atggtccatt
    11701 ggacaagcgg gtcagcggtt tgcaaatgct ggcgaatatg gtctagagat tcttcgattt
    11761 cttgatttga ggcagttctt ccagcaaagt atttggcacg aaggaaccaa cctaatcgtt
    11821 gaaaaatagc ctggtcttgg atgagtaagt attcttccca gtctttgcta tccttgcgtt
    11881 tgctgaggat aatggaagcc aatttatcga ggacattgac cgaaatttgt tcattgaggc
    11941 aattctctag gtcagcttgg ggaagttggt taggtttagc cttggcaact tgaatagcta
    12001 aatattgtgc ttctaggaca cctgtttgcc agagtggggc aaataagtcc agtcgatttt
    12061 tataggcctt ggcgatacca gaaatagctc ccattggaac tccgtagtag ggcttagttt
    12121 cgccaatttt ttcataacgc ttgagggtgc cagcatggct ggctacttca agttgggtga
    12181 gaatgatgtt catttcagtc ataaatatct cccaaaaaaa ctggattgct ccagttttta
    12241 tatgtagtcg tttagtttgc ttttagtaca aggcaacgag gtgcaggttg ctagaacagc
    12301 ctagaggctg ttctaggttg gaaataagac atgcgaagca agtcactttc gtggagtacg
    12361 gcaaagcgag ttaacgaagt aatcaaagct aaactaaatg actatttcaa taaaccacgt
    12421 tcacgatacc agacatcagg tgtccaaacc cacttgcaac tgtagctttc tagcaaatca
    12481 aaggctgcct caggtcccat agttccagct ggataggtgt ggagtgggac ttggttgctt
    12541 gcccacaaat caacaatgcg gtcaatcaag ctccagcttg ctttcacttc ttcccaatgg
    12601 ctaaagttag tagaatcacc atttaggaca tcgaaaaaga gtttttcgta ggcttcgggt
    12661 gaatttccaa gagcagttgc attgtggcgg aagttgagtt tggcaggagt aagagcaaaa
    12721 tgggaaccaa cttccttacc gttgatagag agcatgaagc cttcagttgg ttggatgaag
    12781 atggtcaata cattggcttc ggaaggttga ccaaaaatat cttccgcatg cttgaaggtg
    12841 atggtcacgc gcgtgccctt ttctgtcaga cgtttacctg tacggaagaa gaaaggtaca
    12901 tcacggaaac ggtcagtatc aacgaagaag acacctcctg caaaggtttc tgtctgagaa
    12961 ccttcggcaa tatttggttc atccaagtag ctaacgtagt cttttccgtc aatggaacct
    13021 gctgcatatt gaccacggat gaagttgcgt ttgaggtcct catcagaagg ctgacgaagg
    13081 tgttggaaga ccttaatctt ttcagcgcgg acatcttcct ccttgaagct ggctggctta
    13141 tccattgcta gaagggaaag aacttgaagg gcatggtttt gcaccatgtc ttttaaggcg
    13201 ccagaatgat cgtagtagcc tccacgatcc tcaacaccaa tcgcttcagc aaatgtaatt
    13261 tggacgttat cgatgtaatc gcggttccaa atatgctcaa aaatgatatt ggcaaagcga
    13321 accgcaaaga tattttgcac catttccttg cctaggtaat ggtcgatacg gtagatttgg
    13381 tcttcattga aggctgctgc caattcatca ttgagtttgg tagctgtagc aaggctagtt
    13441 ccgaaaggtt tttcaattat caagcgttca aaaccttgtc catcaacgat ttgctcagat
    13501 ttgaggtgtt tggcaatggt tccgaaaaat tcaggtgcca tagagaggaa gaagaccttg
    13561 ttgtgctggg tattgtattt ttcacacaag tcgtcctgca atttacgaag agctacataa
    13621 tgttccgtgt cattgacatc gtggctttgg tagtagaaat gatttgcaaa ttcatgagct
    13681 tgacgtggtg tgtcaggcag atcacctagt gattcaatga cggtttgctc aaaaaattcc
    13741 ttggtccagg gtctgcgagc tgtaccaatt actgcaaaat tttcccggat atggcctgcc
    13801 ttataaaga
//
LOCUS       DBNFCK010000043        13732 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.043, whole genome shotgun sequence.
ACCESSION   DBNFCK010000043 DBNFCK010000000
VERSION     DBNFCK010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13732
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.043"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            290..964
                     /gene="rpiA"
                     /locus_tag="AC1PIW_001016"
     CDS             290..964
                     /gene="rpiA"
                     /locus_tag="AC1PIW_001016"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HIP6605963.1"
                     /translation="MINLKEQVGIKAAEFVTDGMIVGLGTGSTAYYFVQEIGRRVAEE
                     GLQITGVTTSHATAEHAASLGIPLKNIDEVEYVDLTVDGADEVDGDFNGIKGGGAALL
                     MEKVVAVNSKDCIWIVDESKMVQTLGAFKLPVEVVQYGAENLFRLFEKKGYRPSFRMR
                     NGKKHITDMQNFIIDLDLRRIEDTYALAEELDRTVGVVEHGLFIGLISKVIVGTPEGP
                     NIIEKK"
     gene            1293..2504
                     /locus_tag="AC1PIW_001017"
     CDS             1293..2504
                     /locus_tag="AC1PIW_001017"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - metabolic compound salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HIP6605964.1"
                     /translation="MPKFKRVHLVVMDSVGIGAAPDSDKFFNAGVADTESDTLGHISE
                     KAGLEVPNMTKIGLGNIPRDTALATVPAESHPTGYVTKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWDGFPEEILTKIEEFSGRKIIREANKPYSGTAVIDDFGPRQMETGELIV
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFSRT
                     ANRHDYAVSPFEATVLNKLADAGVPTYSVGKISDIFNGSGITNDMGHTKSNMHGVDVL
                     IDTLKLSDFEEGFSFTNLVDFDAVYGHRRNIEGYRDCLQEFDARIPEIIDNMREDDLL
                     LITADHGNDPSYAGTDHTREYVPLLAYSKAFKGSGVLPVGHFADISATVAENFGVDTA
                     MIGESFLSQLV"
     gene            2504..2860
                     /locus_tag="AC1PIW_001018"
     CDS             2504..2860
                     /locus_tag="AC1PIW_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HIP6605965.1"
                     /translation="MEKLTVYYNPNCSKCKKLRILLSSQDMEVKWVNYLETPLRGTEL
                     TALLEKMGSQPSAVIRLKEEERLALSEEEIFERLVKEPATLNRPIIEREQTAFLCRPL
                     EIIKEKMPEYDWSDYL"
     gene            2872..3681
                     /locus_tag="AC1PIW_001019"
     CDS             2872..3681
                     /locus_tag="AC1PIW_001019"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIP6605966.1"
                     /translation="MSLLAKINETKTFLEEKGLVKPEFGLILGSGLGELAQEVENAIV
                     IDYADIPNWGKSTVVGHAGKLVYGDLAGRKVLALQGRFHFYEGNPMEVVTFPVRVMKA
                     LGCEGVIVTNAAGGIGYGPGTLMAITDHINMTGQNPLIGENLEEFGPRFPDMSNAYTK
                     EYREKAHAVAEKLGIKLDDGVYLGVTGPTYETPAEILAFKTMGAHAVGMSTVPEVIVA
                     AHSGMKVLGISAITNFAAGFQSELNHEEVVEVTEQIKGNFKGLVKAILAEL"
     gene            3714..4427
                     /gene="deoD"
                     /locus_tag="AC1PIW_001020"
     CDS             3714..4427
                     /gene="deoD"
                     /locus_tag="AC1PIW_001020"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIP6605967.1"
                     /translation="MSIHISAKPGEIADKILLPGDPLRAKFIAENFLEDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGIPSISIYAHELINEYGVKKLIRVGTAGSLNEDVHVRE
                     LVLAQAAATNSRMINIDWPEYDLPQIADFDLLDKAYHIAKELNMTTHVGNVLSSDSFY
                     SPKLFSRNLELGQLGVKAVEMEAAALYYLGAKFGVQTLAIMTISDSLVNPEEDTTAEE
                     RQNTFTDMMKVGLETLIAE"
     gene            4602..6869
                     /gene="glgP"
                     /locus_tag="AC1PIW_001021"
     CDS             4602..6869
                     /gene="glgP"
                     /locus_tag="AC1PIW_001021"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HIP6605968.1"
                     /translation="MINLQNFVQSMYAKRIEDCTDQELYYALLAFAKQQSEAKYTNDQ
                     KKKVYYISAEFLIGKLLSNNLINLGLYDEVKSQLVAAGKDLIAIEDMELEPSLGNGGL
                     GRLAACFLDSIASLGLNGDGVGLNYHFGLFRQLFEYHQQSAVPNEWITPRSWLTESPI
                     SYQVPFANFTLTSKLYDIDVPGYKMERKNRLRLFDLGSVDDNIIYDGIEFDKEDIFRN
                     LTLFLYPDDSTDEGKVLRIFQQYFMVSNAAQLLIDEAVAKGSNLHDLPDYAVVQINDT
                     HPSLVIPELIRLLELRGISFDEAVEIVKKMTAYTNHTILSEALEKWPLDFLNYVVPHL
                     VPFIVELDRRAKEVKDDPAVNIIDEHGRVHMAHMDIHYGYSINGVAALHTEILKTSEL
                     KAFYELYPEKFNNKTNGITFRRWLMHANPSLASYLDGLLGHGYHHDASELEKLLDYKN
                     DKELKSWLEKTKQHNKRKLQRHIAKTQGVHVNPESIFDVQIKRMHEYKRQQMNVLYVI
                     HKYLDIKAGNIPTRPLTVFFGGKAAPAYTTAQDIIHLILVLSQVIKNDPDVAPHLQLV
                     MIENYNVTEASYIIPAADISEQISLASKEASGTGNMKFMLNGALTIGTDDGANVEIHE
                     LVGDENIYIFGQDSQTVIEHYANGTYHPYSFYEREAIRPLIDFITSDTIRHAGGIDER
                     LWRLQDNLKHNDHFMTLLDLEDYISTKEQMLADYEDRDSWLEKVIVNIAKAGFFSSDR
                     TIQQYNEDIWHLEVK"
     gene            complement(7004..7240)
                     /locus_tag="AC1PIW_001022"
     CDS             complement(7004..7240)
                     /locus_tag="AC1PIW_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIP6605969.1"
                     /translation="MVFNLLLFLPLGLLFSFSWKKLSLFVGAILLVEACQFFFSLGFF
                     DLGDILLNTSGFALGNLLGKSAIAQSFKNRIQKK"
     gene            7695..8822
                     /gene="hemW"
                     /locus_tag="AC1PIW_001023"
     CDS             7695..8822
                     /gene="hemW"
                     /locus_tag="AC1PIW_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HIP6605970.1"
                     /translation="MKKRPTSAYIHIPFCTQICYYCDFSKVFIKNQPVDDYLVALMEE
                     VKFYDLPALKTLYIGGGTPSALSAEQLDYLLTNLEDLLDLSQLEEFTIEANPGDLTAD
                     KIAVLKKSKCNRVSLGVQTFDDRMLKKIGRSHNQAQIYETIAALKEAGFHNISIDLIY
                     ALPGQTMEQVVDNVAKALELDIPHMSLYSLILENHTVFMNRQRRGNLHLPNEDVESDM
                     FDYILQELEKNGFEHYEISNFTKPGFESHHNLMYWDNSEYYGLGAGASGYIDGMRYRN
                     RAPIQHYLKSIREKGHSRLHEEFLSKTEQMEEEMFLGLRKKTGVSIERFEKKFGISFE
                     DRYGQVVRDLKNEGLLQEEDCWLRMTKKGLFLGDTVAERFI"
     gene            9053..9793
                     /locus_tag="AC1PIW_001024"
     CDS             9053..9793
                     /locus_tag="AC1PIW_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HIP6605971.1"
                     /translation="MGLTYQEEFTIPFDMVDVKQEIKLPDFISYCLGVSGRQSEELGR
                     SDLYVFQEFGLIWVVTDYELTIQRLPKYNETITIKTEAVAYNKFFCHRMFYIYDAAGN
                     LLLDILCYFVLIDFETRKVAPVPEDLIAPYQSEQVKKLPRAPKYQLLENPSVQEFPVR
                     YFDLDMNGHVNNGKYLEWMYEALGYDFLLCHVPKKIQLKYLKEVEATSLVSSRMVAEA
                     GGSQHEIVVDGHIHAQAVIEWRERHVAG"
     gene            9780..10397
                     /locus_tag="AC1PIW_001025"
     CDS             9780..10397
                     /locus_tag="AC1PIW_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HIP6605972.1"
                     /translation="MSQDKWLEWAVRLQALAQTGLAYGKDVYDMERFEEIRQIAAEML
                     VEPSGQPLEVVKDLFCNETGYQTPKLDTRAAIFQEDKILLVQENDGLWSLPGGWCDVD
                     QSVKDNVVKEVKEEAGLDVEAQRVVAILDKHKNNPAKSAHRVTKVFILCRLLGGEFQP
                     NSETVASGFFSLDDLPPLYLGKNTAEQLALCLEASRSEHWETRFD"
     gene            10407..11180
                     /locus_tag="AC1PIW_001026"
     CDS             10407..11180
                     /locus_tag="AC1PIW_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HIP6605973.1"
                     /translation="MTYTGYLIDLDGTIYEGKKRIPAGERFIHRLQECQIPYLFVTNN
                     TTRRPEMVQAMLAENFNIETPLETIYTASLATVDYMNDLGKEKTVYVIGEDGLKSAIF
                     EAGYVEDTENPAYVVVGLDTQLTYEKLTIATLAIQKGATFIGTNPDLNIPTERGHLPG
                     AGSLIALLKAATRVEPTFIGKPEAIIMDKALEILGTERSQTVMVGDNYLTDIRAGIDN
                     GFPTLLVLTGFTKPEEVADLPLAPTHVLNSLDEWSFDEN"
     gene            11170..11799
                     /locus_tag="AC1PIW_001027"
     CDS             11170..11799
                     /locus_tag="AC1PIW_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HIP6605974.1"
                     /translation="MRTKLQIIGTILFVLSAAVLGTIYLAWLVYPLEISFLGLEKVVY
                     MKVADISYNFNILMNYLTNPFASVLDMPNFSSSADGLKHFADVKHLFHLTQGIFILTL
                     PAFVLFVKNILLKGYGDLVKKVIFWTMLTPMIIGLLGVLVGFDQFFALFHTVLFPGDS
                     TWLFDPAKDPVIYILPQEFFLHCFVLFFVLYEFFFWTILAWTGKKNRIG"
     gene            11898..12074
                     /gene="rpsU"
                     /locus_tag="AC1PIW_001028"
     CDS             11898..12074
                     /gene="rpsU"
                     /locus_tag="AC1PIW_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIP6605975.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEAARKRKKF"
     gene            12178..13191
                     /gene="galE"
                     /locus_tag="AC1PIW_001029"
     CDS             12178..13191
                     /gene="galE"
                     /locus_tag="AC1PIW_001029"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HIP6605976.1"
                     /translation="MSILVTGGAGYIGSHTVVELLKLGKEVVIVDNLSNSSILVLDRI
                     ETITGKRPTFYELDVADKEALRQVFENENIEAAIHFAGYKAVGESVAKPIMYYENNIM
                     STLALVEVMTEFSVKKIVFSSSATVYGLNNPSPLVETMPTSATNPYGYTKVMLEQILR
                     DVEVADKEWSIALLRYFNPIGAHESGLIGEDPAGIPNNLMPFIAQVAVGKREELSVFG
                     NDYDTIDGTGVRDYIHVIDLALGHIKALEKISTTTGVYTYNLGSGQGTSVLELVKAFE
                     KVNGVPVHYKLVDRRPGDVATCYANADKAWKELNWKTEKTIEDMCQDTWNWQSKNPNG
                     YEG"
     gene            complement(13282..13647)
                     /gene="mscL"
                     /locus_tag="AC1PIW_001030"
     CDS             complement(13282..13647)
                     /gene="mscL"
                     /locus_tag="AC1PIW_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HIP6605977.1"
                     /translation="MLKDLKAFLFRGNIVDLAVAVVIGGAFGAIIKSFVNDIITPLIL
                     NPALKAANVQNIADLAWNGVKYGSFLSAVINFLIIGTSMFFVVKAAEKAMPKKVEEKA
                     PAGPTQEELLAEIRDLLKK"
BASE COUNT         4062 a         2667 c         3097 g         3906 t
ORIGIN      
        1 taccctttag gtattctttt tccaccttta gcagtaacaa tagcacccaa cctcacccac
       61 tcccagctct ctggtatatc ataaggcact tcaatttctt ggactgtccc atcagctaac
      121 ttctcataag gtttcctacg tccctaagtt agtagcaaat atatttgaac aaatgaaaaa
      181 aattttttag gatttaagac attatttttt gaatttctaa ctcactcttg tcccatctcc
      241 ctctccatgc tatactatat gtattcgttt tacttcaaag gagacaaaga tgataaacct
      301 aaaagagcag gttgggatta aggcggcgga atttgtgacg gacgggatga ttgttgggct
      361 gggaactggt tcgacggcat actatttcgt gcaggagatt ggccgccggg ttgcagaaga
      421 agggctacag ataacaggcg taacgacctc tcatgccacg gctgagcacg ctgcgtccct
      481 tgggattccc ctaaaaaata tcgacgaagt cgaatatgtg gacttgacgg tggatggagc
      541 agatgaggtt gatggtgact tcaatgggat taaaggcggt ggtgcagcgc ttctcatgga
      601 gaaagtggta gcagtcaata gcaaggactg catctggatt gtggatgagt ctaagatggt
      661 tcaaacccta ggagccttca agttgccggt ggaagtcgtg cagtacggtg cggaaaatct
      721 tttccgacta tttgagaaaa aagggtatcg tccaagtttt cgtatgcgta atggaaaaaa
      781 acatatcacg gatatgcaga actttatcat tgaccttgat ttgcgcagaa tagaagacac
      841 atatgcattg gcggaagaat tggatcggac cgtcggtgtt gtagaacacg gtctctttat
      901 tggattgatt tccaaggtaa ttgttggtac accagaagga ccgaacatca tcgaaaagaa
      961 ataaattgaa aacgcttctc attttggtac aatgagaggc gtttttatgg tataatgaaa
     1021 acacgaacgt ttagactctt caaaaatcta tccccttacc tcgtagcagt cagatgatag
     1081 gtactgaaaa agcgatagct gaacagcagg cagtgaagcg agtaacagta tctagttttg
     1141 atggttaaga agtttgatgc cgagagcttt gactgtataa gtattgcacc ccattaaaat
     1201 gtttaggtgt ggtgatgata atttccagtc tagcaacttg gttggacgct gattttggtt
     1261 gaatctaact atatcaaata aaggagacaa ttatgcctaa atttaaacgt gttcacttgg
     1321 tagttatgga ctctgtcgga atcggcgcag caccagactc agataaattt ttcaatgcgg
     1381 gtgtagcaga tacagaatct gacactctcg gccacatttc tgaaaaagct gggcttgagg
     1441 tgcctaatat gaccaagatt ggtcttggaa atatccctcg agatacagcg cttgcgacag
     1501 ttcctgctga aagccatcca actggttacg tgaccaagtt ggaagaagta tcgcttggta
     1561 aggataccat gactggacac tgggaaatta tgggtctcaa tattactgag ccatttgata
     1621 cattctggga tggtttccct gaagagattt tgacaaaaat cgaagaattc tcaggtcgta
     1681 aaatcatccg cgaagcaaac aagccgtatt caggtacagc tgtcattgat gactttggcc
     1741 ctcgccaaat ggaaactggg gagttaattg tctatacctc cgcagaccca gtccttcaga
     1801 ttgcagccca cgaagacatt attccgctgg atgaattgta tcgtatctgt gaatacgctc
     1861 gctccattac ccttgaacgc ccagctcttc ttggtcggat tattgcccgt ccatacgttg
     1921 gtgagccagg caatttctca cgaactgcca accgtcacga ctatgcagta tcaccttttg
     1981 aggcaacagt actcaacaaa ctggcagatg cgggtgtacc aacttactca gttggtaaaa
     2041 tcagtgatat tttcaatggt tcaggtatta caaatgatat gggtcacacc aagtccaaca
     2101 tgcacggtgt cgatgtgttg attgatactc tgaagttatc agacttcgag gaaggtttct
     2161 ccttcacaaa cttagttgac tttgatgctg tgtatggtca ccgtcgcaat atcgaaggct
     2221 accgcgattg tttgcaagag tttgatgcac gtattccaga gattattgac aatatgcgtg
     2281 aagatgacct gctcttgatt actgcagacc acggcaatga tccatcctat gctggaacag
     2341 atcacacacg cgaatatgtg ccactcttag catacagcaa ggctttcaaa ggtagcggtg
     2401 ttttgccagt aggccatttt gcggacattt cagcaaccgt tgcggaaaac ttcggagtgg
     2461 acacagccat gattggcgaa agcttcttgt cacaattggt ttaatggaga agctaacggt
     2521 ctattacaat cctaactgta gcaagtgtaa aaaattaaga atattgctgt ccagccagga
     2581 tatggaagtc aagtgggtta attatttgga aacaccatta aggggaactg aattgacagc
     2641 tctcttggaa aaaatgggca gtcaaccttc agcagttatt cgattgaagg aagaagaacg
     2701 actagccttg tcagaagaag aaatctttga gcgtttagtt aaagagcctg ccactctcaa
     2761 tcgccccatt atcgagcgag agcagacagc cttcctctgt cgtccgcttg aaatcattaa
     2821 agaaaaaatg ccagaatatg actggtcgga ttatttataa aaggagaaaa tatgagttta
     2881 cttgcaaaaa tcaatgaaac aaaaacgttc ttggaagaaa aaggtttggt aaaacctgaa
     2941 ttcggtttga ttttgggttc tggcttgggt gaattggccc aagaagttga aaatgctatt
     3001 gtcatcgact atgcagatat tccaaactgg ggtaaatcaa cagttgttgg tcatgctggt
     3061 aagttggttt acggtgattt ggcaggacgt aaggtcttag ctcttcaagg tcgtttccat
     3121 ttctacgaag gaaatccgat ggaagtcgtg actttcccag tccgtgtcat gaaagctctt
     3181 ggttgtgaag gagtgattgt tactaatgcc gcaggtggta ttggctacgg tccaggtacc
     3241 cttatggcca ttacagacca catcaacatg actggccaaa atccattgat tggcgaaaac
     3301 ttggaagaat ttggtccacg tttcccagat atgtccaatg cctacactaa agaataccgt
     3361 gaaaaagccc atgccgttgc ggaaaaattg ggtatcaagt tggacgatgg tgtctacctc
     3421 ggtgtgacag gccctaccta tgaaacacct gctgaaatct tggccttcaa gaccatgggg
     3481 gcccacgcag tcggtatgtc aacagtacca gaagttatcg tggcagctca ctcaggcatg
     3541 aaagtcttgg gtatttctgc tatcacaaac tttgcggctg gtttccaatc tgaacttaat
     3601 cacgaagaag ttgtagaagt aaccgagcaa atcaagggca acttcaaggg cttggtgaag
     3661 gctatcttgg ctgagttgta agtattcgaa aatagaacaa agaggtacat tacatgtcta
     3721 tccatatttc cgcaaaacca ggcgaaatcg ctgataagat tttgcttcct ggtgatccgc
     3781 ttcgtgctaa atttatcgct gaaaacttcc ttgaagatgc cgtttgcttc aacgaagttc
     3841 gcaatatgtt cggctacaca ggtacctaca agggtcatcg tgtctctgtc atgggaacag
     3901 gtatgggaat accatccata tctatctatg ctcatgaatt gattaacgaa tatggtgtga
     3961 aaaaactaat tcgtgtggga acagcaggtt ctttgaatga ggatgtccat gtccgtgaat
     4021 tggtcttggc tcaggcagct gcgaccaact ctcgtatgat caatattgac tggccagagt
     4081 acgatttgcc acaaatcgct gattttgatc ttttagataa ggcttatcat atcgccaaag
     4141 aactcaacat gacaacgcat gttggcaatg ttttatcatc agatagtttc tattctccaa
     4201 aattgtttag tcgcaactta gaattaggtc aactaggtgt caaggctgta gaaatggaag
     4261 cagctgcact ttattaccta ggtgctaagt ttggcgttca aacgcttgcc attatgacga
     4321 tttcagatag cttggttaat ccagaggaag acaccacagc agaagagcgt caaaatactt
     4381 tcacggatat gatgaaagtt ggtttggaaa ctctgattgc agaataatta agaataattc
     4441 tgattgtagt aacctgcaat cgtttaaaag acgaactaat ttctatttgt acggaagttg
     4501 gcttgtcttt ttttataaat agggtataat taagaaaacg tttacaaagg taaagcgaaa
     4561 cctttctgaa tgattacatt aatatatcta ggagagccct catgattaat ttacaaaatt
     4621 ttgttcagtc tatgtatgcc aaacgcattg aagattgtac agaccaagaa ctctattacg
     4681 ccttgcttgc ttttgcaaaa cagcagagtg aagctaagta tacaaatgac caaaagaaaa
     4741 aggtctacta tatttcagca gagttcttaa ttggtaagct cttgtctaat aatttaatca
     4801 accttggtct ttatgatgag gttaaaagcc agttggtggc agctggtaag gatttgattg
     4861 ctattgaaga catggagctt gaaccgtctc ttggtaatgg aggtttgggg cgcttggctg
     4921 cctgcttcct tgattcaatt gctagtctag gtcttaacgg tgatggggtc ggcttgaatt
     4981 atcactttgg actgtttaga caattatttg aataccatca gcagtctgct gtgccaaacg
     5041 agtggattac accacgttca tggttaacag agtcaccgat ttcttatcag gtgccatttg
     5101 ctaactttac cttgacatct aagttgtatg acatcgatgt acctggttat aaaatggaac
     5161 gcaagaatcg acttcgcttg tttgaccttg gttcagttga tgacaatatc atttatgacg
     5221 gaatagaatt tgataaggaa gatattttcc gaaacttgac cctcttcctc tatccagatg
     5281 attcgaccga tgaaggaaaa gttctccgca tcttccagca atatttcatg gtgtctaacg
     5341 ctgcgcaact attgattgac gaagcggttg ctaagggatc aaacttacat gacctacctg
     5401 attatgccgt tgttcaaatc aacgatactc acccatcact tgttattcca gaattgattc
     5461 gccttttgga gctccgtggt atttcgtttg atgaggcagt tgaaattgtt aagaaaatga
     5521 ctgcttatac caaccatacc atcttgtcag aggcgctgga aaaatggccg cttgattttc
     5581 tgaactatgt ggttccgcac ttggtaccgt tcatcgtaga attggatcgt cgtgccaaag
     5641 aagtgaagga tgatcctgct gtcaatatca tcgatgaaca tggccgtgtt cacatggcac
     5701 acatggatat tcattatggt tattccatca atggggtggc ggcacttcat acagagattt
     5761 tgaaaacttc agagttgaaa gctttctatg agctatatcc tgaaaaattc aataataaga
     5821 caaatgggat taccttccgt cgttggctca tgcatgctaa tccaagtttg gcaagctacc
     5881 tggacggcct acttggacat ggctaccatc acgatgcaag tgagctcgaa aagctgttgg
     5941 attacaaaaa tgataaggaa ttgaaatctt ggttggaaaa gacaaagcag cataataaac
     6001 gcaaattgca gcgccatatt gccaagacac aaggtgttca tgttaatcca gaatccatct
     6061 ttgatgtaca aatcaagcgc atgcacgagt acaagcgtca gcagatgaat gttctttatg
     6121 ttatccataa gtatctggat attaaagcag gcaacatccc tactcgccca ttaacagttt
     6181 tctttggtgg aaaggctgca cctgcttaca caaccgcaca ggacatcatt catttgattt
     6241 tggtattgtc acaggtgatt aaaaatgatc ctgacgtggc gccacacttg caactcgtta
     6301 tgattgaaaa ctataacgta accgaggcaa gttatattat tccagcggca gatatttcag
     6361 aacaaatttc tcttgcatcc aaagaagctt ctggtacagg taacatgaaa ttcatgctca
     6421 acggagcctt gaccatcggt acagatgatg gggccaatgt ggaaattcat gagttggtag
     6481 gtgatgaaaa tatttatatc tttggtcaag atagccaaac agttatcgaa cactatgcaa
     6541 atgggaccta ccatccatat tccttctacg aacgtgaagc cattcgccca ttgattgact
     6601 ttattacatc ggataccatt cgtcatgctg gtgggatcga tgagcgtctc tggcgtttgc
     6661 aggataacct taagcacaat gaccacttca tgaccctgct tgatttggaa gattatatct
     6721 cgactaaaga gcagatgtta gcagactacg aagatcgcga cagctggttg gaaaaagtca
     6781 tcgtcaacat cgctaaagca ggtttcttct cgtcagaccg tactattcaa cagtacaacg
     6841 aagacatctg gcatttggaa gttaagtaag ctaatactct tcgaaaatca aaattatccg
     6901 ttgtcaagag cctagatgaa cttcagttct atcgtcggct tcgtttccta ggctactttt
     6961 gattttcatt gagtataaat aaactaaaaa tgaccttgcg gggtcatttt ttctgtattc
     7021 tatttttaaa agattgagcg attgcggatt ttcccaaaag gtttccaagg gcaaagccag
     7081 aagtgttaag caagatgtcg ccaaggtcaa agaagcccag tgaaaagaag aattgacagg
     7141 cttcaaccag taaaatagca cctacaaaga gagaaagttt tttccaagaa aatgaaaaaa
     7201 gcaacccaag tggtaaaaag agtaagaggt taaaaaccaa tatagtagga ttgagaaata
     7261 ggtcttgtga aaaagtgcta aggagattct agttgacacc tctgacgccc atgcttttga
     7321 aaaagagaag atagactagc agaagcccgt agcaagcgta gaaaaaccaa agtgtcagtc
     7381 gcgaaatgtg gggtttatag acaagtcgaa atagggtaaa gcaaaatccg gtcattaaca
     7441 ggattgcggc aagtgtcggc caaaaatcaa ctgtcatgaa aatggtcgtg aactggtaaa
     7501 aaagaaaggt cataaagagc cagcggcaga ataagaatga taagatagcg ttgatgacat
     7561 catagaatcg ttctttgttc atgatttaat cccttttgta gattttgtaa gtttactata
     7621 ttatatcatt gttttgaggg ggaagtgatg gctgtaaaaa atatttttat agacagtgtg
     7681 ctataatagt tcttatgaaa aaacgaccga catctgccta tatccacatt ccattttgta
     7741 cccagatttg ttactactgt gacttttcca aggtttttat taagaaccag cctgttgatg
     7801 actatttggt agccctcatg gaagaggtca agttttacga ccttccagcc ctcaagaccc
     7861 tctacatcgg aggcggaaca ccgtcggctc tatcggcaga acagttggac tacctcctga
     7921 ccaatctgga ggacttgctg gacttgtcac agctggaaga attcactatc gaggccaatc
     7981 caggtgactt gacggcggat aagatagcag ttctgaaaaa gtccaagtgc aatcgggtgt
     8041 cgcttggcgt tcaaacattt gatgaccgta tgttgaaaaa aattggtcgc agccacaatc
     8101 aggcccagat ttatgaaacc attgcggccc tcaaggaagc gggcttccac aacatttcca
     8161 ttgacctgat ttatgccctg cctggtcaga ccatggagca ggtggtggac aatgtcgcca
     8221 aggccttgga gctagatatt ccccacatga gcttgtatag tttgattttg gagaatcaca
     8281 cggtcttcat gaaccgccag cgtcgcggca atcttcatct acccaacgaa gatgtggagt
     8341 ccgatatgtt tgactacatt cttcaggagt tggagaaaaa cggcttcgaa cactacgaga
     8401 tttccaactt caccaagcct ggttttgaaa gccaccacaa cctcatgtac tgggacaact
     8461 ccgagtatta cggattggga gcaggggctt caggctacat cgacggcatg cgttatcgca
     8521 accgtgcccc catccagcac tacctcaagt ccatccgcga aaaaggccat tctcgcttac
     8581 acgaagaatt tctcagcaag accgagcaga tggaagaaga gatgttttta gggctacgga
     8641 aaaaaactgg cgtgtccatt gagcgattcg agaaaaagtt tggtatctcc tttgaagacc
     8701 gctacggtca ggtggtcaga gacttgaaaa atgaaggact cttgcaagaa gaagactgtt
     8761 ggcttcggat gactaaaaag ggattgtttc tgggcgatac agttgcagag cgtttcatat
     8821 agtcattcag tttgttttta ttacttcgac gagcgaagaa acttcgtttc cttatttcca
     8881 acttcaaaca ctccactgga gtatttgaac tcactttgcc gtactctacg aatgttactt
     8941 actatcgtaa gtacgatttc caaccttcaa tagtccactg gactattgaa gctacctgca
     9001 gctgttgacg tgaactgaaa gactatactt taagactaga aagatcaaca ttatgggact
     9061 aacataccaa gaagagttca ccattccttt tgatatggtg gatgtcaaac aagaaatcaa
     9121 actacctgac tttatttcct actgtcttgg tgtatcaggt cggcagtcgg aagaactggg
     9181 tcgcagtgac ctctatgttt ttcaggaatt tggcttgatt tgggtggtga cagattatga
     9241 actgaccatt caacgtttac ctaagtacaa tgaaaccatt accattaaga cagaagcggt
     9301 tgcctacaac aagtttttct gtcatcggat gttctatatc tacgatgcag caggcaatct
     9361 tttgttggac attctctgct attttgtcct gattgatttt gaaactcgca aggtggcgcc
     9421 tgtgccagaa gatttgattg ctccttatca gtctgagcag gtcaagaaat tgcctcgggc
     9481 tcccaagtat cagctcttgg aaaatccatc tgtccaagaa tttcctgttc gctattttga
     9541 cttggatatg aatgggcatg tcaataatgg caagtatttg gaatggatgt atgaagcact
     9601 gggctatgat tttctgctct gccatgtccc taaaaaaata caactcaagt atcttaaaga
     9661 ggtagaggca actagcttgg tgagttcgcg tatggtggca gaagctggtg gtagtcagca
     9721 tgagattgtg gtagatgggc atatccatgc gcaagctgtc atagaatgga gggaacgtca
     9781 tgtcgcagga taagtggttg gaatgggccg tacgtttaca agccttggct cagacaggct
     9841 tggcctatgg aaaagatgtt tatgacatgg aacgtttcga agaaatcaga cagattgcgg
     9901 cggaaatgtt ggtagagcca tctggacagc ctttggaagt agtgaaagac ttgttttgca
     9961 atgaaacagg ctatcagaca cccaagctgg atacccgcgc agctattttc caagaggata
    10021 aaatcctact ggttcaggaa aacgatgggc tctggtcctt accaggcggt tggtgcgatg
    10081 tggaccagtc tgtcaaggac aatgtagtca aggaagtaaa ggaagaagcg gggctggatg
    10141 tggaggctca acgtgtggtg gcgattttgg acaaacacaa gaacaatcct gccaagtcag
    10201 cccaccgcgt gaccaaggtc ttcattctat gtcgtctcct tggcggggag tttcagccca
    10261 attcggaaac agttgccagt ggctttttca gtctagatga cttaccaccg ctctaccttg
    10321 ggaaaaatac agctgagcag ttagcacttt gcttggaagc tagtcggtct gagcattggg
    10381 aaacacgatt tgattaagga ggaaaaatga catatacagg ttatttgatt gatttggatg
    10441 gtacgattta tgaagggaaa aagcggattc ctgctggtga acgttttatt catcgcttgc
    10501 aagaatgtca gattccctat ttatttgtga ccaacaatac cacccgccgt cctgagatgg
    10561 tgcaggctat gttggctgaa aactttaaca ttgagacacc gcttgaaaca atctatacag
    10621 ccagtctggc gacagtagat tatatgaatg acttgggcaa ggaaaagaca gtctatgtca
    10681 ttggagaaga cgggctcaag tctgccattt ttgaggcagg ttatgtggaa gacacggaaa
    10741 atccagccta tgttgttgtc ggtttggata ctcagttgac ctatgaaaaa ctgaccattg
    10801 ccaccttggc tattcaaaag ggagcgacct ttatcggtac caatcctgat ttgaacattc
    10861 cgactgaaag aggtcatttg ccaggagcag gctcacttat tgcgcttttg aaagctgcta
    10921 cccgagtaga gccgaccttt atcggtaagc ctgaagccat tatcatggac aaggctctgg
    10981 agattttagg cacagaacgc agccagacag ttatggtagg agataactac ctgacagata
    11041 ttcgtgcagg gattgacaat ggatttccaa ctcttttagt cctgacagga tttaccaagc
    11101 cagaagaagt agcagactta cccctggctc caacccatgt cctcaacagc ctagatgaat
    11161 ggagttttga tgagaactaa gttacagatt atcggaacga ttttatttgt cttatcggca
    11221 gcagttctag gaaccatcta cctagcttgg ctagtttatc cgcttgagat ttcttttttg
    11281 gggttggaaa aagtggtcta catgaaggtg gcagatattt cctacaattt caatatcctg
    11341 atgaactatc taacaaatcc ttttgcaagt gttttagaca tgccaaattt ttcatcctca
    11401 gctgatggtc tgaaacattt tgcggatgtg aaacatttat tccatctaac tcagggaatt
    11461 tttatcctca ccctccctgc ctttgtgctt tttgtaaaaa acattctgct aaaaggttat
    11521 ggagacttgg ttaaaaaggt cattttctgg accatgctta cgccaatgat aatcggttta
    11581 ttaggagttc ttgttggctt tgatcagttt tttgcattgt ttcatacggt gctatttcca
    11641 ggagattcta catggctatt tgaccctgca aaggatcctg tcatatatat tttgccccaa
    11701 gagtttttct tgcattgctt tgtattattt tttgtccttt acgaattctt cttttggaca
    11761 atactggcgt ggacaggaaa aaagaatagg ataggataag agcgtaaaat tcatatttta
    11821 gaatgaaaaa aatattgaca aatgagttga atcatagtaa aatgataaag actttgaaat
    11881 tgaggggagt gaaaaaaatg tcaaaaacag tagtacgtaa gaacgaatca cttgatgatg
    11941 ctcttcgtcg tttcaaacgt gcggttacta aagctggtac tcttcaagaa acacgcaaac
    12001 gcgaattcta cgaaaaacca tctgtaaaac gtaaacgtaa atcagaagca gctcgcaagc
    12061 gtaaaaaatt ctaattgaat ttgaaaacgg ccgaaaggct gttttttgtt tactttttac
    12121 aacaggtaac atataatagg gatatagaat tttgtccagg tgaaaaggag taaaaagatg
    12181 agtatcttag ttactggtgg tgcaggttat attggtagtc atacggttgt cgaattgttg
    12241 aagttgggga aagaagtggt tatcgtggat aatctttcaa actcaagtat tttggtttta
    12301 gatcgcattg aaaccatcac tggcaagcgc ccaactttct atgagttgga cgtggcagat
    12361 aaggaagcct tgcgccaagt ttttgaaaat gaaaacattg aggcagccat tcactttgct
    12421 ggctacaagg cagtaggaga atcagtggct aagccaatta tgtactatga aaacaatatc
    12481 atgtcaaccc tagctttggt agaagtcatg acagaattta gtgttaagaa aattgtcttc
    12541 tcctcaagtg cgactgttta tggtctcaat aatccatcgc ctttggttga aactatgcca
    12601 actagcgcaa cgaatccgta cggctatacc aaagttatgt tggaacaaat cttgcgtgat
    12661 gtggaagtag cagataagga gtggagtatt gcattgctcc gttatttcaa tccaatcggt
    12721 gcccatgaat ctggcttgat tggggaagat ccagctggta ttcctaataa tttgatgccg
    12781 tttattgcac aggtagcagt tggtaaacgt gaggaactca gcgtttttgg taacgattat
    12841 gatacaattg atggtactgg agtgcgtgat tatatccatg tgattgattt ggcactcggt
    12901 catatcaagg cgcttgaaaa aatttcaact actacaggtg tatatactta caatcttggt
    12961 tcaggtcaag gaacaagtgt attggagttg gtcaaagcct ttgaaaaggt aaatggcgtt
    13021 ccagtacact ataaacttgt tgaccgtcgt ccaggtgatg tggcaacctg ttacgccaac
    13081 gcagacaagg cttggaaaga gttaaattgg aaaacagaaa aaacaattga agacatgtgt
    13141 caagatacgt ggaactggca atcgaaaaat ccaaatggat acgaaggata agaaaagatg
    13201 tgagtggagg ctcacatctt atttttgtaa ttatggaaga gaaaaacagg tatggagacc
    13261 tgtttttcaa tatattattg attatttttt caataaatcg cggatttctg caagcaactc
    13321 ttcttgagtt ggaccagcag gagctttctc ttcaactttc ttaggcattg ctttctcagc
    13381 tgctttaaca acaaagaaca ttgaagtgcc aatgattagg aagttaataa cagcgctcaa
    13441 gaaactaccg tacttcacac cattccatgc aaggtcagca atattctgta catttgccgc
    13501 tttcaaagct gggttcaaaa tcaatggagt gatgatatca tttacaaatg atttgataat
    13561 tgcgccgaac gctccgccaa ttaccactgc tacagcaagg tcaacaatgt tcccacggaa
    13621 caaaaatgct tttaaatctt tcaacatatc ctaaatatgt cctttcataa atattttact
    13681 ttatctatta tacccataaa aaaatatttt acaagcaatt tgttttagaa at
//
LOCUS       DBNFCK010000044        13625 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.044, whole genome shotgun sequence.
ACCESSION   DBNFCK010000044 DBNFCK010000000
VERSION     DBNFCK010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13625
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.044"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..355
                     /locus_tag="AC1PIW_001031"
     CDS             <1..355
                     /locus_tag="AC1PIW_001031"
                     /inference="COORDINATES: protein motif:HMM:NF022158.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HIP6605978.1"
                     /translation="MFENLFDPDGSLVWHTALFYSFSAIILATVCFHIIKNFMEWHHN
                     NRAPKESCKAKLVSKRTHVFGNEVARTNYYVTFEWEGKRQEFRLRSDEYALLAERDVG
                     TLHFQGTRFLGFERL"
     gene            440..817
                     /locus_tag="AC1PIW_001032"
     CDS             440..817
                     /locus_tag="AC1PIW_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HIP6605979.1"
                     /translation="MIQSIGQVMLYVNDIEASAQFWKEKMGFERVEKQVQGPQTSYII
                     APKSDSEVQFVLHDKAEVAEMEPELNLGTPSILMTSVDVAKTYEELVERGVTTNPVMD
                     LGFMKVISFADNDGNYYAIREVK"
     gene            818..1363
                     /locus_tag="AC1PIW_001033"
     CDS             818..1363
                     /locus_tag="AC1PIW_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605980.1"
                     /translation="MSLQNRVEEMYKDHEVKPYISPERDLAAWLLEAKPVPKRNMVRL
                     EEGILPGDIILLWRISLGSFESTTPYSKYFEYMYGINGPAHMEQLIADGYAYVESAFD
                     SLDHITSTAKKNILKAEGVTGLSKMKAADLDTALKDNLTEEKLAPYFTVRGYALTEKG
                     RAALDNHPEVLAKHPMKKMYK"
     gene            1520..2509
                     /locus_tag="AC1PIW_001034"
     CDS             1520..2509
                     /locus_tag="AC1PIW_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIP6605981.1"
                     /translation="MEEQKSITMKDVARVAGVSVGTVSRVINNEPGIKESTLEKVNAA
                     IKELNYIPDVYARGMKKNKTETIALIIPTVWHPFFGEFAYHVEVELSKKNYKLLLCNI
                     SGPKRELDYLTMLQQNKVDGIIAITYSPIEDYLSSNIPFVSIDRTYENKAIPCVSSDN
                     RAGAELAANTLIAKGGRHFAFIGGHNKTINETKKRRLYFEKRILEAGFPCHVLDLEEP
                     YDDFVGQVEEFLINNPQVDAIFTINDFTALDTLAVLEKLRRRVPEDVQVIGYDGIQLA
                     SEKSLALSTIRQPLESMAQEAVACLIDIIEKRERPLQVTLPISYLEGKTTKNF"
     gene            2614..3534
                     /locus_tag="AC1PIW_001035"
     CDS             2614..3534
                     /locus_tag="AC1PIW_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6605982.1"
                     /translation="MKTSKLQQASLFDRIKQQKLLLLMLLPGLVLTFIFRYIPMYGVL
                     IAFKDYNPLKGVLGSEWIGFEEFTKFLSSPNFGTLLANTLKLSVYGLLLGFLLPIILA
                     IMLNQLLSDKAKKRIQLVLYAPNFISVVVIVGMIFLFFSVGGPVNSILGMFGIEANFL
                     TDPDFFRPLYILSGIWQGMGWASTLYTATLINVDPALIEAAKLDGANIFQRIWHIDLP
                     ALKPVMVIQFILAAGGIMNVGYEKAFLMQTSLNLTSSEIISTYVYKIGLVSGDYSYST
                     AVGLFNALINIILLIAVNKIVKRINDGQGL"
     gene            3544..4431
                     /locus_tag="AC1PIW_001036"
     CDS             3544..4431
                     /locus_tag="AC1PIW_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIP6605983.1"
                     /translation="MNTQMYTKFDKRMLVVNKILLAFLVLITVVPMVYILVASFMDPQ
                     ALLSKGISFDPKDWTVEGYQRVFSDKSILRGFFNSLFYSFSFAFVTVSISVMTAYPLS
                     KKDLVGKKWINAFLVFTMFFGGGLVPTYLLVKDLGMLNTVWAIIIPGAVNVWNIILAR
                     TYFQGLPDELVEAAVMDGANEFQIFWKIMIPLAKPIMFVLFLYAFVGQWNSYFDAMIY
                     IKDPDLEPLQLVLRKILIQSEPAKDMIGAQAAMNEMKRIAEMIKYATIVISSLPLIVM
                     YPFFQKYFDKGIMAGSLKG"
     gene            4478..6088
                     /locus_tag="AC1PIW_001037"
     CDS             4478..6088
                     /locus_tag="AC1PIW_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIP6605984.1"
                     /translation="MKHTFGKTVVTLLASTVLLAACGSKNTASSPDYELKDVQFPLEK
                     SVTLKFMTASSPLAPADSNEKLIFKRLEEETGVHIDWTNYQSDFGEKRNLDIASGDLP
                     DAIHNDGASDVELMNWAKQGVIVPVEDLIDKHMPNLKKILDENPEYRSMITAPDGHIY
                     SFPWIEELGEGKESIHTVNDIAWINKEWLDKLGLDMPKTTDDLIKVLEAFKTQDPNGN
                     GKADEIPMGFINGGGNEDFKILFGAFGVGDNDDHLVVNNDGTVDFTADNEDYKEGVEF
                     MRTLQEKGLLDSEAFEQDWNTYIAKGSENLCGVYFTWDKANITGMNDTYDILPVLAGP
                     DGTKHITRTNGMGFQRDRMVITSANKNLELTAKWIDAQYKPIQSVQNNWGTYGDETQQ
                     NIFELDTATNSLKHLPLEGTAPGELRQKTEVGGPLAILDEYYGTVTTMPDDAKWRLDL
                     MHATYLDYVTNDDIYPRVFMEQEDLDKIAQVEADMNDFIYRKRAEWIVNGGIEEEWDS
                     YLKELESYGLSDWLAIKQKYYDQYKENQ"
     gene            6106..7464
                     /locus_tag="AC1PIW_001038"
     CDS             6106..7464
                     /locus_tag="AC1PIW_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HIP6605985.1"
                     /translation="MKTVVEANQFIQKEKGNVNPIFKPQAHLTPETGWMNDPNGFIYF
                     RGEYHLFYQFNPYESVWGPMHWGHAKSKDLVNWEQLPVDLAPDKAYDKDGCFSGSAIV
                     KDDVLWLMYTGHIVNEDGTVSQVQNMAFSTDGIHFEKIEQNPVATADGLPEEVIANDF
                     RDPKIFEKDGHYYSVVATKHKDNVGCIVLLSSPNLTDWKFESIFLKGEANQGFVWECP
                     DYFEVDGQEYLIISPMRYQKDGNDFININSNIFVTGHVDWDKKVFVAESFKEIDHGHD
                     FYAAQTTEGPEGERVMIAWMHTWGRPLVTNDLGHKWYGQMTLPRLLKQGENGLRQVLP
                     AGILNSFDDIEIGQVIQGPSKLSLKLDDSLDLKLGTDRDYLQFGYDKAKQEVYIDRSH
                     LKIQQVGEEEWSTVRRAVTVQATELEVLVDTNCLEIFINDGQETLTSTFYVEGQIILN
                     AL"
     gene            7590..9959
                     /locus_tag="AC1PIW_001039"
     CDS             7590..9959
                     /locus_tag="AC1PIW_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIP6605986.1"
                     /translation="MRKTSKKGKATRRPTKAELAQQERVKNITLRVLGLLFIAFAASR
                     LGVFGVTSYNIFRLLFGSLAYLLLVGAFIYLLIPKVLREREGTISGFWLIVIGLLIEF
                     QAYLDWTYQGSDLFGHTLKLALSDLAKFQVTAFLGGGMIGSIFYLPVSFLFANVGSFF
                     IGLLVIAFGIFFVSPWSVYDVADGLAVAKDKLVETQVKRAELRTQKKAEREEKRQQEL
                     ARLEEERLRLEEEEESARALALVDRHVNLETGEILEEEPVQVPIISEYDHLDIEDEFP
                     IMLAEDDQPAQSNSASQDSETNEFEKDETDVQIDFKPKQRLAYKLPSIDLFAPIKAKS
                     QSNEKRIVRQNIKVLEDTFASFGIKVVVERAEIGPSVTKYEVKPAVGVRVNRISNLAD
                     DLALALAAKDVRIEAPIPGKSLVGIEVPNSEVATVPFRELWEQSKTDPDKLLEIPLGK
                     AVNGSVRSFNLARMPHLLVAGSTGSGKSVAVNGIISSILMKAGPDQVKFMMIDPKMVE
                     LSVYNDIPHLLIPVVTNPRKAARALQKVVDEMEKRYELFSQIGVRNLEGYNAKVEEFN
                     SRSEEKQIPLPLIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGIHMILATQRPSV
                     DVISGLIKANVPSRIAFAVSSGTDSRTILDENGAEKLLGRGDMLFKPIDENHPVRLQG
                     SFISDDDVEAIVGFIKDQADADYDESFDPGEVAEGDGDAGFGDAGGDPLFNEARALVV
                     ETQKASASMIQRRLSVGFNRATRLMEELEAAGVIGPAEGTKPRKVLETQ"
     gene            9959..10345
                     /gene="gloA2"
                     /locus_tag="AC1PIW_001040"
     CDS             9959..10345
                     /gene="gloA2"
                     /locus_tag="AC1PIW_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMU1112c/YaeR family gloxylase I-like
                     metalloprotein"
                     /protein_id="HIP6605987.1"
                     /translation="MKFDAVHHIAIIGSDYGKTREFYVEKLGFEQVDEHIRPEKNDIL
                     FNVKKGNLILEIFIKPDAPMRPAMPNPEHTGLRHLAFQVADVEACLEEFDRLDIRHEV
                     LRTDDFDGKKMAFFFDPDGLPLEIHE"
     gene            complement(10713..11081)
                     /locus_tag="AC1PIW_001041"
     CDS             complement(10713..11081)
                     /locus_tag="AC1PIW_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HIP6605988.1"
                     /translation="MFESIIFLKIVAFLFIFLTLAISIIAVKFFRLQNRGWNFADIAF
                     PLYAIEFYLISDKAYYNSLLPQLVLALSLLAIGLCGFFLLKKKNFLYRRFFKVFWRAS
                     FILTFLMYLALVIAVFTLKS"
     gene            11236..11661
                     /gene="rplK"
                     /locus_tag="AC1PIW_001042"
     CDS             11236..11661
                     /gene="rplK"
                     /locus_tag="AC1PIW_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HIP6605989.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFITKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAASLEAAMRMIEGTARSMGFTVTD"
     gene            11702..12391
                     /gene="rplA"
                     /locus_tag="AC1PIW_001043"
     CDS             11702..12391
                     /gene="rplA"
                     /locus_tag="AC1PIW_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HIP6605990.1"
                     /translation="MAKKSKNLRAALEKIDSTKLYSVEEAVALAKETNFAKFDASVEV
                     AYNLNIDVRKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINGGWLDFDVVIATPDMMAVVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADKAGIVQALIGKVSFDADKLVENFKAFHDVMAKAKPATAKGTYMTSVSITTTQ
                     GVGIKVDPNSL"
     gene            12498..12788
                     /locus_tag="AC1PIW_001044"
     CDS             12498..12788
                     /locus_tag="AC1PIW_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6605991.1"
                     /translation="MKVAEKEELYKYLSAAYNLPQEAFSEALREKILEVAGQLDKEEN
                     LYILAGHLSRFINAELTALTCRAPKELVQLAHYLQEVQNHYRYASLFPGKVK"
     gene            12797..13585
                     /gene="yghU"
                     /locus_tag="AC1PIW_001045"
     CDS             12797..13585
                     /gene="yghU"
                     /locus_tag="AC1PIW_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HIP6605992.1"
                     /translation="MTEYTKPLVWKWEDENDNRSGNRPTAGSRFEQDLPRGEKSFQVY
                     SLGTPNGIKVAIMLEELKELGISEADYDLFLINIGQGDQFGSDFVAINPNSKIPAMVD
                     YSEQEPISVFESANILLYLAEKFEAFLPKAWAERTEVLNWLFWQTGAAPFVGGGFGHF
                     FHYAPTAQEYPINRYTMETKRQLDLLDQLLATREYIAGNTYTIADIAIWSWYGRLVQG
                     ELYPGSAEFLDVDSYKHLKTWVDKIAQLPAVQRGLTVKYHSIDN"
BASE COUNT         4145 a         2719 c         2974 g         3787 t
ORIGIN      
        1 gatgtttgag aacttgtttg acccagatgg aagcctagta tggcatacgg cccttttcta
       61 cagtttttcc gcaatcatat tagcgacagt ttgtttccat atcattaaaa attttatgga
      121 atggcaccac aacaatcggg cgcccaaaga atcttgcaag gctaagcttg tctctaaacg
      181 aacccatgtt tttggtaacg aagtagcacg aaccaattat tatgtaacgt ttgaatggga
      241 gggaaaacgg caggaatttc gccttcgttc agatgaatat gcccttttag cagagagaga
      301 tgtaggcacc ctgcattttc aggggactcg tttcctaggt tttgaacggc tctaagagtc
      361 gtttttcttt ggagaatatc cctatctagt gtataatggt ttgaacacat gttcgtacaa
      421 cgcaaaagag gtatttaaga tgattcaatc aatcggacaa gttatgttat atgtgaatga
      481 tattgaggct tcagctcaat tttggaaaga gaaaatggga tttgaacgtg tagaaaaaca
      541 agtgcaaggc ccccaaactt cttacattat tgcacctaaa tcagatagtg aggtacaatt
      601 tgtacttcat gataaggcgg aggttgctga gatggagcca gaattaaact taggtacacc
      661 atcaatctta atgacatcag tagatgtagc caaaacttac gaagaacttg ttgaacgtgg
      721 tgtcacaacg aatccagtca tggaccttgg tttcatgaag gtaattagct ttgcagacaa
      781 tgatggaaat tattacgcaa ttcgtgaggt aaagtaaatg tcactccaga atcgtgttga
      841 agaaatgtat aaagaccacg aggtcaaacc ctatatttcc cccgagcgtg acttggcagc
      901 ctggctgttg gaggccaagc ccgttcccaa acgcaatatg gttcgtctgg aagaaggtat
      961 tttgccagga gatattattt tgctatggcg gattagcctg ggttcttttg aatcgacgac
     1021 gccctactcc aagtactttg agtacatgta tggtatcaat ggtcctgctc acatggagca
     1081 gttgatcgca gacggttatg cctatgtgga atcggccttt gattccctag accacatcac
     1141 ttctactgcc aagaaaaata tcctcaaagc agagggtgtg acaggcctat ccaagatgaa
     1201 ggcggcagac ctcgacactg ccctcaagga caacttgacg gaagaaaagt tagctccata
     1261 ctttaccgtc cgaggttatg ccctgacgga aaaaggcaga gctgcactcg ataatcaccc
     1321 agaagtgctt gcaaaacacc caatgaagaa gatgtacaaa taaaatagaa aagattttta
     1381 aacggcactc aaaagttaga atcattctag ctttttgggt gtttttattt gctaacaagt
     1441 caatttttct tgacagaaaa cgctatcaat gatattctag tgtaaattat tgaaacgttt
     1501 caaaagaaag gggtaaacca tggaagaaca aaaatcaatt actatgaaag atgtagctcg
     1561 agttgcaggg gtcagtgttg ggacagtttc aagagttatc aacaatgagc caggtattaa
     1621 ggaaagtacg cttgaaaaag tcaatgctgc cattaaggaa ttgaattaca ttccagatgt
     1681 ttatgctcgt ggcatgaaga agaacaaaac cgaaactatc gccttgatta ttccgacagt
     1741 ttggcatcct ttttttggag aatttgccta tcatgttgaa gtggaactga gtaagaaaaa
     1801 ttacaagttg ctcctttgca atatttctgg tccaaaaagg gagttggatt atctgactat
     1861 gctgcaacaa aataaggtgg acggcattat tgctatcacc tacagtccaa tcgaggatta
     1921 cttatcttct aacattccat tcgtcagtat cgaccgcacc tatgaaaata aggctatccc
     1981 ttgtgtcagt tcggacaacc gagctggtgc ggaactggca gcgaatacct tgatagcaaa
     2041 aggaggtcgc cattttgcct ttatcggtgg gcacaataaa acaatcaatg aaaccaagaa
     2101 gcgtagattg tattttgaaa agaggatttt agaggcaggt tttccttgtc atgtcttgga
     2161 cttggaagag ccatatgatg actttgtcgg tcaagtcgaa gagtttctga ttaacaatcc
     2221 acaggttgat gctattttca ccatcaatga ttttacggcc ctagacacac ttgctgtttt
     2281 ggaaaaactg agacgacggg ttccagaaga tgtgcaggtg attggctatg atggtattca
     2341 gttggcaagt gaaaaatctt tagccctttc gaccattcgt caacctttgg agtctatggc
     2401 ccaagaagcc gttgcctgtc taattgatat tattgagaaa agagagcgcc ccctacaagt
     2461 cactctaccg atttcctatc ttgaaggaaa aactacaaaa aatttttaaa aaagcagttg
     2521 acagaaaacg gttacaattg taaaataaaa ccatcaataa acagttggtt ttattttcac
     2581 aagaaaaatg aaacgtttca aaaaggagtt cttatgaaaa catcaaaact gcaacaagct
     2641 agtctattcg atagaatcaa gcaacagaaa ctcttgttac tgatgttact accaggcttg
     2701 gtcttgactt tcatcttccg ttacattccc atgtacggag tgctgattgc ctttaaagac
     2761 tataatcctt taaaaggggt gcttggtagt gagtggattg ggtttgaaga gtttaccaag
     2821 tttctctcgt ctccaaattt cggcacctta cttgccaaca ccttgaaatt gagtgtctac
     2881 ggtctgctct taggatttct tctaccaatt attttggcca tcatgctcaa tcaactattg
     2941 agcgataagg ctaaaaaacg gattcagttg gtgctctacg ctccaaactt tatctcagtc
     3001 gttgtcatcg tcggtatgat tttcctcttc ttctcagtag gaggaccggt taattccatc
     3061 ctaggtatgt ttggcattga agccaacttc ctgacagacc cagacttctt cagacccctc
     3121 tacatcctca gtggtatttg gcagggaatg ggctgggcat cgactcttta cacggctacc
     3181 ttgatcaatg ttgatccagc cttgattgaa gcggctaagc tagacggtgc aaatattttc
     3241 caacgaatct ggcatattga cttaccagca ctaaaaccag ttatggtcat tcagtttatc
     3301 ttagcagctg ggggcatcat gaacgttggt tacgaaaaag cctttctgat gcagacttcc
     3361 ctcaacctga ccagttctga aatcatttca acctatgtct ataaaatcgg tttggtgtca
     3421 ggggactact cttattcaac agcggttggc ttatttaacg cacttatcaa tattattctg
     3481 ctgattgcag tcaataagat tgtcaaacgc atcaacgacg gacaaggctt ataggaggtt
     3541 cgtatgaata cacaaatgta cacaaaattt gacaagcgaa tgctggttgt caataaaatc
     3601 ttgctggcat ttcttgttct cataacagtt gtgcctatgg tctatatctt agtagcgtcc
     3661 ttcatggatc cacaggcctt acttagcaag ggaattagct ttgatccaaa ggactggaca
     3721 gtagaaggtt atcaacgggt attttcagac aagtctatcc tacgcggatt tttcaactcc
     3781 ctcttttatt ccttctcctt tgccttcgtg accgtgtcta tttcagtcat gactgcctat
     3841 ccactctcta aaaaagattt ggtcgggaaa aaatggatca atgccttcct tgtcttcact
     3901 atgttcttcg gtggtggact tgttccgacc tatctcttgg ttaaagattt gggaatgttg
     3961 aatactgtct gggccattat tattcctggt gcagtcaatg tgtggaatat catcttggca
     4021 cggacctatt tccaaggctt gccagatgag ttggtagaag cggccgtaat ggacggtgct
     4081 aatgaatttc agattttctg gaaaatcatg attccactag ctaagccaat tatgtttgtg
     4141 ctcttcctct atgcctttgt cggtcaatgg aattcttact ttgatgccat gatttacatc
     4201 aaggacccag acttggagcc actccaacta gtacttcgta aaatcctcat tcaaagcgag
     4261 cctgctaagg acatgattgg tgcccaggct gccatgaatg aaatgaaacg aatcgcagaa
     4321 atgattaagt atgcgaccat tgtcatctct agcttgccac tcattgtcat gtatccattc
     4381 ttccagaaat actttgacaa ggggattatg gctggttcac taaaaggtta aaattgttca
     4441 aaatttttat agaaaacaaa acaggaggtt gtttctgatg aaacacacat ttggaaaaac
     4501 agttgttaca cttcttgcaa gtaccgtttt attggctgct tgtggctcaa aaaatactgc
     4561 ttcaagccct gattatgaat tgaaagatgt tcaattccct ttggaaaaat ccgttacact
     4621 taagtttatg acagctagtt cacctctagc tccagccgat tcaaatgaaa aattaatttt
     4681 caagcgttta gaggaagaaa ctggcgttca tattgattgg acaaactacc agtctgattt
     4741 tggtgaaaag cggaacttgg acatcgcttc aggtgatttg ccagacgcca tccataatga
     4801 cggggcttct gatgtggaat tgatgaactg ggctaaacaa ggtgttattg taccagttga
     4861 agacttgatt gataagcata tgccaaacct caagaaaatt cttgatgaaa atccagagta
     4921 ccgttccatg attacagcac cagacggtca tatctactca ttcccatgga ttgaagaatt
     4981 aggagagggc aaggagtcta tccataccgt taatgacatt gcttggatta acaaggaatg
     5041 gttggacaaa cttggacttg atatgccaaa aacaacagat gacttgatta aggttctcga
     5101 agcctttaaa acacaggatc caaatggaaa cggtaaggct gatgaaattc cgatgggctt
     5161 cattaatggt ggaggtaacg aggactttaa aattctattt ggtgcctttg gtgtaggcga
     5221 taacgatgac cacttagttg tcaacaatga cggtaccgtt gactttacag cagacaacga
     5281 agactacaaa gaaggtgttg agttcatgcg tacccttcaa gaaaaaggtc ttctagattc
     5341 ggaagcgttt gaacaagatt ggaatactta cattgccaaa ggtagtgaaa atctatgcgg
     5401 tgtttacttc acttgggata aagctaacat tacaggtatg aacgatactt atgacatctt
     5461 gccagtcttg gcaggtcctg atggaaccaa gcacatcaca cgtacaaacg gtatgggctt
     5521 ccaacgcgac cgcatggtta tcacctcagc taacaaaaac ttggaattga ctgctaaatg
     5581 gattgatgcc caatacaaac caatccagtc tgttcaaaac aactggggta cctatggtga
     5641 tgaaacccaa caaaatattt ttgaactcga cacagcaacc aatagtttga aacacctacc
     5701 attggagggt accgcaccgg gtgaacttcg tcagaagaca gaagttggtg gaccgttggc
     5761 tattcttgat gaatactacg gaaccgtaac aaccatgcca gacgatgcta aatggcgttt
     5821 ggacttgatg cacgcaacct atcttgacta tgtaacaaac gatgatattt accctcgcgt
     5881 cttcatggaa caagaagatt tggataagat tgcccaagtt gaagcagata tgaacgactt
     5941 catctaccgt aaacgtgcag aatggattgt aaacggcggt atcgaagaag aatgggatag
     6001 ctatttgaaa gaattggaaa gctatggtct ttctgactgg ttagctatca agcaaaaata
     6061 ctatgatcag tataaagaaa atcagtaatc aaaaggaggc tgcctgtgaa aacagtagta
     6121 gaagccaatc aatttattca gaaagaaaaa ggaaacgtta atccgatttt caaaccacaa
     6181 gcccatttaa caccggaaac aggctggatg aacgacccca atggatttat ctacttccgt
     6241 ggagaatacc atttgttcta ccaattcaac ccttacgaga gtgtttgggg accgatgcac
     6301 tgggggcatg cgaaaagtaa ggacttggtc aactgggaac aacttccagt tgacctagct
     6361 cctgataaag cttacgataa agacggctgt ttctcgggct ctgccattgt caaagatgat
     6421 gtcctctggc tcatgtacac aggtcacatc gtcaatgaag acgggacagt tagtcaagtt
     6481 cagaacatgg ccttctcgac tgacggtatt cactttgaaa aaatcgagca gaatccagtc
     6541 gcaacagcag atggactgcc agaagaagtg attgccaatg acttccgcga tccgaagatt
     6601 tttgagaaag atggtcacta ctactctgtt gtcgcaacca agcacaagga caatgtcggc
     6661 tgtatcgtct tactcagctc tcccaacctg acggattgga aatttgagtc tatcttcttg
     6721 aaaggtgagg ccaaccaagg ctttgtctgg gaatgtcctg actactttga agtggacggt
     6781 caagagtacc tgattatctc tccaatgcgt taccaaaaag acgggaatga ctttatcaat
     6841 atcaactcca atatctttgt gacgggtcat gtggactggg acaagaaagt ctttgtggca
     6901 gaatccttca aggaaatcga ccatgggcat gatttctatg cagctcaaac gactgaaggt
     6961 ccagaagggg aacgtgtcat gattgcctgg atgcatacat ggggacgtcc gcttgtaacc
     7021 aatgacctag gccataagtg gtatggtcaa atgaccctgc ctcgtctact taagcaaggt
     7081 gagaatggac tcaggcaggt cttgccagca ggtatcttga acagctttga cgacattgaa
     7141 atcggtcaag ttattcaagg accaagtaag ctctccctca aactggacga cagtctagac
     7201 ttgaaactgg gtactgaccg agactatctt caatttggct atgataaagc caaacaagaa
     7261 gtctatattg atcgtagtca cctcaaaatc cagcaagttg gtgaggagga atggtcaact
     7321 gttcgtcgag cggtcacggt tcaagcgaca gagctagagg tcttggttga caccaactgt
     7381 ttggaaatct ttattaacga tggtcaagaa accttgacat caaccttcta cgtagaaggt
     7441 cagattattc taaacgctct ataatattat ttgccctctt aaattattct ttcactccaa
     7501 gagaaaccct gtatttgcag ggttttttct atcatgaaaa ttcttcccga ttatgataaa
     7561 ataaagagat tgagaaagtt gaggtatcta tgagaaagac aagtaaaaaa ggaaaggcta
     7621 cgaggcgacc aaccaaggca gaattagcac agcaagaacg tgttaaaaat atcacccttc
     7681 gagtattggg gcttttattc attgcctttg cagccagtcg cctaggtgtg tttggagtga
     7741 ccagctacaa tattttccgg ctattatttg gtagtctggc ctatctatta ttggtgggtg
     7801 catttatcta ccttcttatt cccaaagtct tacgcgaaag agaagggacc atatcgggct
     7861 tctggctgat tgttatcggg ttattgattg aatttcaggc ttacttagac tggacctatc
     7921 aagggagtga cttattcggt catactctaa aattggcctt gtcagaccta gctaagtttc
     7981 aggtaacagc atttctaggc ggagggatga ttggaagtat cttctatctg ccagtctcct
     8041 tcctctttgc aaatgtcggt tccttcttca tcggtcttct agtgatagca tttggtatct
     8101 tttttgtaag cccatggtct gtttatgatg ttgccgatgg cttggcggtt gccaaagata
     8161 agttggtgga gacgcaggta aaaagagcag agcttcgaac acagaaaaaa gcagaacgag
     8221 aagaaaaacg tcagcaagaa ttagctcgtc tagaagagga acgccttcgt ctagaggaag
     8281 aggaagagtc agctcgggct ttggcactgg tagatagaca tgtcaattta gaaacgggtg
     8341 aaatcctcga agaagagcct gttcaagtac cgattatttc agaatatgac cacttagaca
     8401 tagaagatga atttcctatt atgttggcag aagatgacca accagcacaa tcaaactcgg
     8461 caagccagga ttctgaaaca aatgagtttg agaaggacga gacagatgtg cagattgatt
     8521 tcaagcctaa gcagagacta gcatataaac ttccgtccat agacttattt gctcctataa
     8581 aagctaagag tcagtccaat gaaaaacgaa ttgttcgtca gaatattaaa gttttagaag
     8641 acacctttgc cagttttgga atcaaggtag tagttgagcg agctgagatt ggaccatcgg
     8701 taaccaagta tgaagtcaaa ccagcagtcg gtgttcgtgt caatcggatt tctaacctag
     8761 ctgatgattt ggctctagcc cttgcggcta aagatgtccg tattgaagct cctatcccag
     8821 gaaaatcact ggtcggtatt gaagttccaa actccgaagt ggctacagta cctttccgtg
     8881 agctctggga acaatctaaa acagacccag acaaacttct ggaaattcct ctcggtaagg
     8941 ctgtcaatgg ttccgtccgt tcctttaacc tagcacgtat gccccatcta ttggtggctg
     9001 gttcaactgg ttcagggaag tctgttgctg tcaacggtat catctcttca attctcatga
     9061 aagctggtcc agatcaagtt aagttcatga tgattgaccc caaaatggtc gaattatcgg
     9121 tctataatga tattccccac ctattgattc ccgttgtgac caacccacgc aaggctgctc
     9181 gagctcttca aaaagtagtg gatgaaatgg agaaacgcta tgagttgttt agccaaattg
     9241 gtgtccgaaa cttagaaggt tataatgcga aggtcgaaga atttaatagc cgttcagaag
     9301 aaaaacagat tccacttcct ctaattgtgg taatcgtaga tgagttggca gacttgatga
     9361 tggtagccag caaggaagtg gaagatgcca ttattcgctt gggacagaag gctcgtgcag
     9421 caggtatcca catgattctt gctacccagc gaccatctgt tgatgttatt tcaggtttga
     9481 ttaaagccaa tgtaccatct cgtatcgcct ttgccgtatc aagcggtaca gacagtcgga
     9541 cgattctaga tgaaaatggt gctgaaaaac tcttgggccg cggtgacatg ctctttaaac
     9601 cgattgatga aaaccatcca gtccgcttgc aaggttcctt tatatccgat gatgatgtag
     9661 aagcaatagt tggctttatc aaggatcagg cagatgcaga ttatgatgaa tcctttgatc
     9721 caggtgaagt ggcagaaggt gatggcgatg caggttttgg tgatgctgga ggagatccac
     9781 ttttcaacga agcacgtgcc ttggtagttg agacacaaaa agccagcgcc tctatgattc
     9841 aacgacgctt gtctgttggt tttaacaggg caacacgcct catggaagag ctagaagcag
     9901 cgggtgtcat tggtccagct gaaggaacaa aacctagaaa ggtattggaa acacaataat
     9961 gaaatttgat gcagttcatc atattgcaat tatcggtagt gactatggca aaacaagaga
    10021 attttatgtt gagaaactag gctttgaaca ggtggatgaa catatccgac cagaaaaaaa
    10081 tgatattctc ttcaatgtca aaaaaggcaa tctgatcttg gaaattttca tcaaaccaga
    10141 tgcgccaatg cgtccggcca tgcccaatcc tgaacataca ggcctacgcc atctagcctt
    10201 ccaggttgca gatgtggaag cttgtctgga agagtttgac cgtttggaca tccgacatga
    10261 agtcctgcga acagatgatt ttgatggtaa aaaaatggcc ttcttctttg atccagatgg
    10321 cctccctcta gaaattcatg aataagtcca tcttttcatg cccagagagg cctatcaata
    10381 tgacaactaa ttaatactct tagaaaatca aaatcatacg ctgttgactt gatttgatga
    10441 aggtcagttc tatctatagt catttgaatt aactttgata catcgtcact ttcggcttgc
    10501 cgtactctgc agaagtgact tgctttgaaa gttatatctc caacctagca cagccactag
    10561 gctgtgctag caacctgcac ctcagttcct tgtctgaaag ctaattcatt cgactataca
    10621 tctacgtcgc cttgtctgat ttagtataaa aagaatcccc actgcgcctg caatggggat
    10681 ttattatgcc atctatgaaa ggatggatga ttttaggatt ttaaagtgaa gaccgcaata
    10741 accaaagcta gatacatcaa aaaagtcaag ataaaactag ctcgccagaa aaccttgaag
    10801 aaacgtcgat ataaaaaatt ctttttcttt aaaaggaaga aaccgcaaag accaatcgct
    10861 agtagtgaga gggctaggac aagctgaggc agtagactgt tatagtaggc cttgtccgaa
    10921 atcagataaa attcaattgc atagagagga aaggcaatgt ctgcaaagtt ccatcctcgg
    10981 ttttgcaacc taaaaaactt gacagcaatt atcgaaatgg ccaaggttag aaagatgaat
    11041 agaaaggcta ctatttttaa aaatataatc gattcaaaca tacattcatt ctataaaaaa
    11101 tgtgaaaaaa agtaaaccta aagcttgcat ttctttgaaa ttcgtgtata atgaaaaggt
    11161 atgtgtaaaa cacatatttg tgggaggtaa aaatctttaa ttaccgccaa aaccacaata
    11221 ggaggatttt atatcatggc taaaaaagtc gaaaaactcg taaaacttca aattcctgca
    11281 ggtaaagcta ctccagctcc accagtcgga ccagcacttg gtcaagcagg tatcaacatc
    11341 atgggattca caaaagagtt taacgctcgt acagctgacc aagctggtat gatcatccca
    11401 gtagttatct ctgtgtatga agataaatca ttcactttca tcactaaaac accaccagct
    11461 gctgttcttt tgaaaaaagc tgcaggtgtt gaaaaaggtt caggtacacc aaacaaaact
    11521 aaagttgcaa cagttactcg tgcacaagta caagaaattg ctgaaactaa aatgcctgac
    11581 ttgaacgctg catctcttga agctgcaatg cgtatgatcg aaggtactgc tcgttctatg
    11641 ggattcactg ttactgacta agtagcaccc aagattatct gattacaagg agacatataa
    11701 catggctaaa aaaagtaaaa acttgcgtgc tgctcttgaa aaaatcgaca gcacaaaact
    11761 ttacagcgta gaagaagctg ttgctcttgc aaaagaaact aacttcgcta aatttgatgc
    11821 gtcagttgaa gttgcttaca acttgaacat cgacgtacgt aaagcagacc aacaaatccg
    11881 tggtgcaatg gtattgccaa acggtactgg taaaacttca cgcgttcttg ttttcgcacg
    11941 tggtgctaaa gcagaagaag caaaagctgc tggtgcagac tttgttggtg aagatgacct
    12001 cgttgctaaa atcaacggtg gttggttgga cttcgacgtt gttatcgcaa ctccagatat
    12061 gatggctgtt gttggacgtc ttggtcgtgt acttggtcca cgtaacttga tgccaaaccc
    12121 taaaactggt acagtaacaa tggatgttgc taaagcagtt gaagagtcta aaggtggtaa
    12181 aatcacttac cgtgctgaca aagcaggtat cgtacaagct cttatcggta aagtatcatt
    12241 tgacgctgac aaactcgttg aaaacttcaa agctttccac gatgtaatgg ctaaagctaa
    12301 accagctaca gctaaaggta cttacatgac ttcagtatct attacaacaa cacaaggtgt
    12361 tggtatcaaa gttgatccta actcacttta atcaatagaa agattgtctt agggcaatct
    12421 ttttttcttg agattttctt gacaagcact tgatttcgat ttatactata ttcaatttac
    12481 ataaaaggag ttatccgatg aaagtagcag agaaagaaga gctttataaa tacctttctg
    12541 cagcctacaa ccttccgcag gaagcatttt cagaagccct gcgtgaaaaa atattggagg
    12601 tggcaggtca gctggacaag gaggaaaatc tctatatact ggcaggacac ttaagtcgct
    12661 ttatcaatgc agagttaaca gctctgactt gccgagcgcc taaggaattg gtgcaactag
    12721 cccattacct tcaagaggtt caaaaccatt atcgctatgc tagtcttttt ccaggaaagg
    12781 taaaatagga gaaaacatga cagaatatac aaaaccactc gtttggaagt gggaagatga
    12841 aaatgacaac cgatctggca accgtccaac agcaggcagt cgctttgagc aagacctacc
    12901 aaggggagag aagtctttcc aagtctattc attaggaacg ccgaatggca ttaaggttgc
    12961 tattatgttg gaggagttga aagagttagg tatttcggaa gctgattatg acctatttct
    13021 catcaatatc ggtcaaggag accaatttgg ctccgatttt gttgccatca accccaactc
    13081 caaaatacca gctatggtag attattccga gcaagaaccg atttcggtct ttgaatcagc
    13141 caatattctc ctctacttgg cagaaaagtt tgaggccttc ctgccaaaag cttgggcaga
    13201 gcggacagag gttcttaatt ggctcttctg gcaaactggc gccgctccat ttgttggtgg
    13261 aggatttgga catttcttcc attacgctcc gacagcgcaa gaatatccga ttaatcgcta
    13321 tacaatggaa actaagcgtc agctagatct cttggatcag ctattggcga caagagaata
    13381 tatagctgga aatacctata ccatcgcaga tattgctatt tggtcttggt atggtcgctt
    13441 ggtgcaagga gaactatatc caggttcggc agaattttta gatgttgatt cttacaagca
    13501 tctaaagacc tgggtagata aaatagctca gctcccagct gtacaacgag ggttgacagt
    13561 gaaatatcat tcaattgata attaattgac ttgcccacct tatgggtggg tttttctttt
    13621 acttt
//
LOCUS       DBNFCK010000045        13594 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.045, whole genome shotgun sequence.
ACCESSION   DBNFCK010000045 DBNFCK010000000
VERSION     DBNFCK010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13594
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.045"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..2288
                     /locus_tag="AC1PIW_001046"
     CDS             <1..2288
                     /locus_tag="AC1PIW_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HIP6605993.1"
                     /translation="QNQQTNHFKTKTSASNWEIFRRNVFTSFNALNFAIFLALLAVQA
                     WSNLFFFGVIVLNAVSGMLTEWRARRMIDKLNLMNKDFIRVVRDSETISIAPEDIVLD
                     DVLLLSAGEQVPSDAIVLEGIAETNEAMLTGESDLIVKNTGAELLSGSYLVSGQIYAK
                     VIHVGAENYANKLMLEAKTHKPIVSRILYNMDKIAKFTGKIIIPFGLALFLEAFFIKI
                     LPLKDSVITSSTALLGMLPKGIALLTITSLLTAVIKLGMKNVLVQEMYSVETLARVDV
                     LCLDKTGTITQGKMTVENLLPLTNHYSLDTIQQILATYIQTSEDTNSTAQAIRKEYGD
                     LEHHYKASHIIPFSSDRKWGAMTIENIGHIFLGAPEMLLTQNPPSVSEAQARGSRVLI
                     LALSQQSLLSSQNQLPENIEPLALLEIADPIREDAAETLAYLRSQEVTLKIISGDNPV
                     TVSHIAREAGFADYDSYIDCSKVDDEELIARAETTAIFGRVSPHQKKLLIQTLKAQGH
                     TTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLDSEFRDIPEILFEGRRVV
                     NNISHIAPIFLIKTIYSFLLGLICIASIALGKAEYLLVFPFIQVQMTLIGQFVEGFPP
                     FILTFERNIRPVEKHFLRKSLLLALPNALMVVLSVLIFHLMQVFGYLNLHDMQTLSYY
                     VLGSTGLLAVIRACLPLTKARLALIIYSVFGFFISSHFLHGLIEIHPLNSHTLPIYSG
                     LMLIFIPVFFWISYKQGAFKN"
     gene            complement(2319..3017)
                     /locus_tag="AC1PIW_001047"
     CDS             complement(2319..3017)
                     /locus_tag="AC1PIW_001047"
                     /inference="COORDINATES: protein motif:HMM:TIGR02206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HIP6605994.1"
                     /translation="MSDFFSNQISEAPNLNVLEHLFLMIIWFFMLHLCFRCYQKRWFH
                     IVFWWLQFIQIVSLYTWYIVADSPLSESLPFYHCRLAMLFILWAKPGPLKRYFAYLGL
                     FGSLAAFIHPVFDPFAFPHLTFFTFVIGHYALTVNCMLYLLSDLEGEMLKGKEVVKYT
                     LIMNMLILGVALLTGGNYGFLRQAPLVNTNNLPLNFFLVTCLLCFSILSIQAMLIAYL
                     KKESKQMNSHILKK"
     gene            complement(3108..4469)
                     /locus_tag="AC1PIW_001048"
     CDS             complement(3108..4469)
                     /locus_tag="AC1PIW_001048"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HIP6605995.1"
                     /translation="MKLTLHEVAQVLGAKNDISLYTDTAFNKVEFDSRLVTTGDLFVP
                     LKGARDGHDFIPVAFENGCVVTLSEVSLDVPHILVEDCLAALQQLAAYYLEKTGVEVI
                     AVTGSNGKTTTKDMIHDVLATTYKTYKTQGNYNNEIGLPYTVLHMPDDTEKLVLEMGQ
                     DHLGDIHLLSEIAKPSLSVITLFGEAHLEFFGSREEIAKGKLQIADGMEPGSKLLIPA
                     DPIADKLLPSHVDLIRFGEQADLQVTSLVESKDSLTFTVNFMDGDIFLPVTGKYNATN
                     AMVASYVGKTLGVPDEAIKSALASLNLTRNRTEWKKAANGADILSDVYNANPTAMRLI
                     LETFTSIPANEGGKKIAVLADMKELGSQSVDLHNQMILSLSPDVLDTVIFYGQDIEGL
                     AQLASQMFPIGKVYFFRKNAEQDQFEDLVKQVRESLGKEDQILFKGSNSMNLGKVVEA
                     IEK"
     gene            complement(4598..4978)
                     /locus_tag="AC1PIW_001049"
     CDS             complement(4598..4978)
                     /locus_tag="AC1PIW_001049"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIP6605996.1"
                     /translation="MYQTTVKGEGLFQALSQGYGESVRTFGVTEKGETPVSLVNIGLA
                     ACITMCVQGYYASQEGNKTMPVQVESRLDNQRFEVLIGIGESVSAQKQKEILAYVEKK
                     CKVKALLREDLQFETSFYVLESVE"
     gene            complement(5066..6112)
                     /locus_tag="AC1PIW_001050"
     CDS             complement(5066..6112)
                     /locus_tag="AC1PIW_001050"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HIP6605997.1"
                     /translation="MSKETLILLYGGRSAEREVSVLSAESVMRAINYDKFFVKTYFIS
                     QTGDFIKTQEFSQTPSADEKLMTNATIVEEQKIRPSDIYEEKAVVFPVLHGPMGEDGS
                     IQGFLEVLRMPYVGTNILSSSVAMDKITTKRVLESAGISQVPYVAVIEGENIDDKIAE
                     IEGKLTYPVFVKPANMGSSVGISKAEDLAGLRKALDLAFKYDSRVLVEQGVNAREIEV
                     GLLGNADVKTTLPGEVVKDVAFYDYDAKYIDNKITMEIPAKIDESIMDIMRDNAAKAF
                     RAIGGCGLSRCDFFLTEDGDIFLNELNTMPGFTQWSMYPLLWDNMGLAYPDLIEELVR
                     LAKEMFEKRESHLI"
     gene            complement(6217..6813)
                     /gene="recR"
                     /locus_tag="AC1PIW_001051"
     CDS             complement(6217..6813)
                     /gene="recR"
                     /locus_tag="AC1PIW_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIP6605998.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMEDDVVNEFA
                     KNLLAAKRDLSYCSICGNLTDQDPCAICQDSTRDQSTILIVEDSRDVTALENIQEYHG
                     LYHVLHGLISPMNGIGPDDINLKTLLTRLMENEVTEVIVATNATADGEATSMYISRVL
                     KPAGIKVTRLARGLAVGSDIEYADEVTLLRAIENRTEL"
     gene            complement(6822..8897)
                     /gene="pbp2b"
                     /locus_tag="AC1PIW_001052"
     CDS             complement(6822..8897)
                     /gene="pbp2b"
                     /locus_tag="AC1PIW_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP2B"
                     /protein_id="HIP6605999.1"
                     /translation="MKKRKNKPLEHDGNFISLRLNILFSIVIFLFLVLILRLADMQII
                     NHDFYSNKLSTASQKIISNGSIRGQIYDAQGKPLVENEIQQVVSFTRPNKMSAQEMKE
                     VANKLLQWVNVSDVTITQRDKADYYLADEEVYRQVVEALPKDKKYDSDGNYLSESTIY
                     SNAVDSIPAEALQYSEQDNKAIELFKQMNGATYFSTVNLVTDTLTSEQVAHIVANESQ
                     LPGISTTNNWQRTILPTSLSSIIGTVTSEQAGLPAEDAEYYLSKGYSSNDRVGTAYLE
                     KQYEEVLQGQREKKQINLDRNGNIESIETIQEGQQGNNIKLTIDLAFQDGVNAILRKY
                     FESELATGSALYSEGVYAVALNPSTGAVLAMSGYSHEKGSSTITEDALGTITSVFTPG
                     SIVKGATISSGWENGVISGNQIQLDEPIYFAGSAPITSWYGAYGSFNIDATEALEYSS
                     NIYMVKIALGLLGQTYSANMYLNDGDTLKNAMTKLRSTFAEYGLGASTGIDLPLESTG
                     FLPDKYTTANFITNAFGQFDNYTPMQMAQYAATIANNGTRISPHLVEGIYGNNAQGGL
                     GELIETVSGKEMNQVNISAEDMSLLRQGFYQVVNGSGRFNTGSAIGRGAAVTISAKTG
                     TAETYTTTASGDVVTAVNTNVVAYAPSDNPQIAVAVVLPNLTNQSSTTTKTITQEIIN
                     LYQSLHPMN"
     gene            complement(8974..9723)
                     /locus_tag="AC1PIW_001053"
     CDS             complement(8974..9723)
                     /locus_tag="AC1PIW_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIP6606000.1"
                     /translation="MMGSYDSQAKSMAKTAKHLEKRIERLDKVEQPRKETWVKMEAKG
                     ALDTGLHSLFRLQAGQVLMDEVYLFDVPQIGMTFGEKLALVGANGSGKTSFVRKLFRK
                     ELAGYYNPKLKIAYFAQDLINLDEEKTAFENVSCTSLQDRMMILNLLGMLGLSCDKAQ
                     QKVANLSGGERVRLSLAKVLLADANLLILDEPTNFLDMAAIEVLETFLKEYEGSVPLI
                     SHDQQFVETSVHSQWEIVQACLVKKSQENIF"
     gene            complement(9886..10428)
                     /locus_tag="AC1PIW_001054"
     CDS             complement(9886..10428)
                     /locus_tag="AC1PIW_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIP6606001.1"
                     /translation="MVGNNGVGKSTFLKILLGLDRDFAGQIEVKADWAYVPQLQERSS
                     LSGGEQVWKSIQEAFAQRPQLLIMDEPTANLDQEHQEKLIKQIKRYRGSLLVVSHDRH
                     FLNQIASHIWHLEEEKVQVYLGNYEAFVESRRARREGQQESYEAYQKKVAQMKKAQHE
                     RQAKAQKMGKRGSGIEVNQL"
     gene            complement(10536..10691)
                     /locus_tag="AC1PIW_001055"
     CDS             complement(10536..10691)
                     /locus_tag="AC1PIW_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606002.1"
                     /translation="MEVIKCIRLEKEFAGRRGWAKSLTSLLRVRVNISAQWLIGRFVR
                     ASHTLQI"
     gene            complement(10947..11750)
                     /locus_tag="AC1PIW_001056"
     CDS             complement(10947..11750)
                     /locus_tag="AC1PIW_001056"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIP6606003.1"
                     /translation="MIGKGFNYSILASGSSGNCFYLETDKKKILVDAGLSGKKITSLL
                     AEIDRKPEDIDAILVTHEHSDHIHGIGVLARKYGMDIYANELTWQAMESKLGKIDVAQ
                     KHIFELGAMKTFGDLDIESFGVSHDAACPQFYRFMKDDKSFVMLTDTGYVSDRMVGIV
                     ENADAYLIESNHDIEILRAGSYSWNLKQRILSDKGHLCNEDGADAMIRSLGNRTKKIY
                     LGHLSKENNIKELAHMTMVNQLAQADLGVGVDFQVYDTSPDTATPLTKI"
     gene            complement(11757..13106)
                     /gene="vicK"
                     /locus_tag="AC1PIW_001057"
     CDS             complement(11757..13106)
                     /gene="vicK"
                     /locus_tag="AC1PIW_001057"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HIP6606004.1"
                     /translation="MINQLRYLMTTAEFWFVVILIGFLIALTVLLIENYWDNKQIKQL
                     NQKVNALIEGNYADLLDMRGSPEITDMANSLNDLSEVIRLTHDNLEQEKTRLTSILSY
                     MSDGVIATDRIGRIIMINDMAQKQLGLSNPKQEQYHLLEVLDLSDRYTLRDLLAQTPE
                     IVIDHTNENEEFLTLRANFATIRSESGLISGLVVVLHDMTEQAKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALTESVAPSFVKVSLDETNRMMRMITDLLSLSRIDNQVG
                     QIDVELINFTAFVTFILNRFDQMKNADSDKVYTIVRDYQISPIWVEIDTDKMTQVLDN
                     ILNNAIKYSPDGGTITFSMKTTDSQLIVSVSDEGLGIPKADLPRIFDRFYRVDKARSR
                     AQGGTGLGLAIAKEIVKQHKGFIWAKSEYGHGSTFTIVLPYSKDIALDEWDDSDEEE"
     gene            complement(13099..>13594)
                     /locus_tag="AC1PIW_001058"
     CDS             complement(13099..>13594)
                     /locus_tag="AC1PIW_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIP6606005.1"
                     /translation="SNVPILMLSAKDSEFDKVIGLEIGADDYVTKPFSNRELQARVKA
                     LLRRSELAETQTNIESTGTPELVIGDLVILPDAFVAKKHGKELELTHREFELLHHLAK
                     HLGQVMTREHLLETVWGYDYFGDVRTVDVTIRRLREKIEDTPSRPEYILTRRGVGYFI
                     KGND"
BASE COUNT         3930 a         3174 c         2483 g         4007 t
ORIGIN      
        1 ttcaaaacca gcaaaccaat cattttaaga caaagaccag tgccagtaat tgggaaatct
       61 tcagacgaaa tgtgtttaca tcttttaacg cactaaattt tgccattttc ttagccttac
      121 tagctgttca ggcatggtcc aaccttttct tcttcggagt tattgtcctc aacgctgttt
      181 caggaatgct gacagaatgg cgagcacgac gaatgattga caaactcaac ctgatgaaca
      241 aggattttat ccgagttgtt cgcgatagcg agacaatatc tattgcacca gaagatattg
      301 tcttagatga tgttctcttg ctgtctgctg gcgaacaagt tcctagtgat gctattgttt
      361 tagaaggtat agccgagaca aacgaggcca tgttaaccgg tgaaagcgac ttgattgtga
      421 aaaatactgg ggctgaatta ttgtctggta gctatctagt tagcggacag atttatgcaa
      481 aagtcatcca tgttggcgca gaaaactatg ccaataagct catgcttgag gcgaaaactc
      541 acaaacctat cgtctctcgt attctttaca atatggataa aattgctaaa tttacaggaa
      601 aaattatcat tccattcgga cttgctcttt ttctagaagc ctttttcatc aaaattcttc
      661 ccttaaaaga ctcagttatt accagctcta ctgccctact aggtatgtta ccaaagggaa
      721 ttgctctgct gaccatcact tctctcttaa ctgccgttat caaactggga atgaaaaatg
      781 tcctcgtaca agaaatgtat tccgtcgaaa cacttgcccg agttgatgtt ctttgtttgg
      841 ataagaccgg aacgattact caggggaaaa tgaccgtcga aaacctgctc cccttaacca
      901 accattattc acttgacact atccaacaga tactcgctac ctatatccag acaagtgagg
      961 atactaattc aacagctcag gccattcgaa aagaatatgg tgatttagag caccactata
     1021 aagctagtca catcatccca ttttctagtg atagaaaatg gggggcaatg acaatagaaa
     1081 acattggtca catttttcta ggggcaccag aaatgttact aacacaaaat cctccttctg
     1141 tttctgaggc acaggctcgt ggttcccgtg tcctgattct tgcgcttagc caacagtccc
     1201 ttctttcttc tcaaaatcag ctacctgaga atattgaacc tctagctcta ttagaaattg
     1261 ccgacccaat ccgagaagac gccgcagaaa cactggccta tcttcgctcg caagaagtga
     1321 cactaaaaat aatctctggt gacaatcctg tgacagtctc acacattgcc agggaagctg
     1381 gatttgctga ctacgatagc tatatcgatt gttccaaagt tgacgacgaa gaactaattg
     1441 ccagagctga aacaaccgcc attttcggtc gcgtatcccc acatcagaaa aaattgctca
     1501 tccaaaccct caaagcacag ggacatacta ctgcaatgac cggtgatggg gtcaatgata
     1561 ttcttgcact acgcgaagct gattgttcca ttgttatggc agaaggcgat cccgctacca
     1621 gacaaatcgc taatctagtc ctcctagatt ctgaatttcg cgatattcct gagattctct
     1681 ttgagggacg acgcgttgtt aataacatct cccacattgc tcccatcttt cttatcaaaa
     1741 caatctactc tttcttactt ggccttatct gtatcgctag tattgcccta ggtaaagcag
     1801 aatatttgtt agttttccca tttattcaag tacaaatgac cctcattggt caatttgtcg
     1861 aaggttttcc tccttttatc ctaacttttg aacgaaatat ccgccctgta gaaaaacatt
     1921 ttctcagaaa atctcttctc ttagccctac caaatgctct catggttgtt ctcagtgtcc
     1981 ttattttcca tttaatgcaa gtatttggat acttaaactt gcatgacatg caaacccttt
     2041 cttattatgt tcttggttca acaggtctac tggctgtcat tcgtgcctgt ttgccactga
     2101 caaaagctcg actggctctt attatttatt ccgttttcgg tttcttcatc agctcccatt
     2161 tcctacatgg tctgattgaa atccaccccc taaacagtca cacattgcca atctattctg
     2221 gattaatgct catttttatc cctgtctttt tctggattag ctacaaacaa ggcgcattta
     2281 aaaactaaaa ataagacttg aaaaactaat ttcaagtctt attttttcaa tatatgcgaa
     2341 ttcatttgtt tgctttcttt tttcaaatag gcaatcagca tagcttgtat actcaaaata
     2401 gagaaacaga gtaggcaggt taccaagaag aaattcaaag gaagattatt agtattcact
     2461 aaaggtgcct gtctcaaaaa tccataattt ccaccagtta aaagagctac tcctagaatc
     2521 agcatattca taataagcgt atatttcact acctcttttc ccttcaacat ctccccttct
     2581 aaatcagata acaggtacaa catacaatta accgtcaagg cgtagtgacc aataacaaaa
     2641 gtaaagaagg tcagatgtgg aaatgcaaat gggtcaaata ctggatgaat aaaggccgca
     2701 agcgatccaa aaagaccgag ataggcaaaa taacgtttca aaggtccggg cttcgcccaa
     2761 agaataaaga gcatggccaa gcgacaatga taaaaaggaa gactctctga aagaggagaa
     2821 tcagctacga tataccaagt ataaagggag acaatctgta taaattgaag ccaccaaaaa
     2881 acaatatgaa accatctttt ttgataacaa cgaaaacaaa gatggagcat gaaaaaccaa
     2941 ataatcatga gaaacagatg ttccaatacg ttcaggttcg gtgcttcaga aatctgattg
     3001 ctaaaaaaat cactcatctt attaaaatcc tctctttcct caaaagttag tatatcaact
     3061 tttcgatgtt ttgacaagac ttgctaaaaa agagaggtct ctcctctcta cttttctatt
     3121 gcttccacta ctttcccaag attcatggaa ttagagcctt taaagaggat ttggtcctct
     3181 tttcctagac tttccctaac ctgtttaacc agatcttcaa attgatcctg ctctgcattt
     3241 ttcctaaaga aatagacttt tccaatcggg aacatctgac tagccaactg ggccagacct
     3301 tcaatatcct gaccgtagaa aatgacggtg tctagcacat caggcgacag gctcaaaatc
     3361 atctgattgt ggagatcgac ggactggctc cccagttctt tcatatctgc taaaacggcg
     3421 attttcttgc cgccttcgtt tgcaggaatg ctagtgaaag tttccaaaat caatcgcatg
     3481 gccgttgggt tggcgttgta gacatcgctg agaatatctg ctccgtttgc tgccttcttc
     3541 cactcggtgc ggtttcgggt caagtttaga ctggctaggg ctgacttgat tgcctcatca
     3601 ggcacaccca aggtcttacc tacgtagctt gctaccatgg cattggtggc attgtactta
     3661 cccgttactg ggaggaaaat gtcgccgtcc ataaaattga cggtgaaggt caggctatct
     3721 tttgactcga ccagactggt cacctgcaaa tcagcctgct caccgaagcg aatgaggtct
     3781 acatggctcg gcaagagctt atccgcaatt ggatctgctg ggatcaagag tttactacct
     3841 ggctccattc catctgcaat ctgtaatttt cctttggcaa tctcttctcg tgagccgaaa
     3901 aattctaaat gcgcttcacc aaaaagggta ataactgata gacttggttt ggcaatttcg
     3961 gatagaagat gaatatctcc caagtggtct tggcccattt ccaagaccag tttttctgta
     4021 tcatcaggca tgtggaggac ggtgtatggc agaccgattt cgttgttgta gttgccttgt
     4081 gtcttataag tcttgtaagt cgtcgccaga acatcgtgaa tcatgtcctt ggtggtcgtt
     4141 ttaccgtttg atcctgtcac agcaatgact tctacccctg ttttctctag gtagtaagcc
     4201 gctaattgtt gaagcgcagc caagcagtcc tctactagaa tgtgaggaac atcaaggcta
     4261 acttcagaca gggttactac gcagccattt tcaaaagcca caggaataaa gtcatgaccg
     4321 tcacgcgcac ctttaagggg aacaaagagg tcgccagttg tgacgagtcg actatcgaac
     4381 tcgaccttgt tgaatgcagt atcggtataa agactaatat cattttttgc gcccaagacc
     4441 tgagcgactt cgtgtagggt taatttcatt gttttctcca ataaactaaa aaagagcttt
     4501 cgctctttct attataccac tttttatcga acttgagtga ggacaccatc tttcatctca
     4561 taaaccttgt cgcatttctt caattgctaa aaacatctta ctccacactt tctaagacat
     4621 aaaaacttgt ctcaaactgc aagtcttccc tcaaaagagc tttgacttta catttctttt
     4681 ccacataggc aagaatctct ttctgcttct gcgctgaaac cgactcacca attccaatca
     4741 acacctcaaa acgctgattg tccaagcgac tctccacctg aacaggcatg gttttattgc
     4801 cttcttggct ggcatagtag ccctgcacac acatggtgat acaggcagct aagccaatat
     4861 taactagact aaccggagtc tcacccttct ccgttacccc aaaggtacga accgattcgc
     4921 catatccttg agaaagagcc tgaaacagac cttccccttt tactgtcgtt tgatacataa
     4981 acacctccag aacttgttcc attttatttc tttttgcaaa cctttgcaag aaaaaagatt
     5041 ggaatgattc caatcttgat aataattaaa tgaggtgact ttcgcgtttt tcaaacattt
     5101 ccttagccaa acgaacaagc tcttcaatca agtctggata agctaggccc atattatccc
     5161 aaaggagtgg atacatagac cattgggtga aacctggcat ggtattgagt tcgtttagga
     5221 agatgtcgcc gtcttcagtc aggaagaaat cacagcgaga aagtccacaa ccaccgatag
     5281 cacggaaggc cttggcagca ttatcacgca tgatatccat gatagactca tcaatcttag
     5341 ctgggatttc catggtgatt ttgttgtcga tgtatttggc atcgtagtca taaaaagcaa
     5401 cgtctttgac tacttcgcca ggaagtgtcg ttttcacgtc agcatttccc aaaagaccga
     5461 cctcaatctc acgcgcatta acaccctgct caacgaggac acggctatca tacttgaagg
     5521 ccagatcaag tgccttacgc aagccagcta aatcctcagc ttttgagata ccgacacttg
     5581 accccatatt agctggtttt acaaagaccg gataagtcaa ttttccttca atctctgcaa
     5641 tcttatcgtc gatattttca ccctcgataa ctgccacata aggtacctgg gagattcctg
     5701 ctgattcaag tacgcgcttg gtagtgattt tgtccatagc aacgcttgaa gacaagatat
     5761 tagtgccaac ataaggcata cgaagcactt ccaagaagcc ttgaatagat ccgtcttctc
     5821 ccattgggcc atgaagaaca gggaaaacaa ccgccttttc ttcataaata tcgctcggac
     5881 ggattttttg ctcttcaaca atcgtcgcgt ttgtcatgag tttttcatcg gcagacggtg
     5941 tttgagaaaa ctcttgcgtc ttaataaagt ccccggtctg gctaatgaaa taggttttga
     6001 caaaaaattt atcataattg atagcacgca tgacgctttc ggcagacaga acagatacct
     6061 cacgctctgc agaacgccca ccatatagta aaatcaaggt ttcttttgac atatattttc
     6121 cttcctgtat caacgctatt actcttagta tagcataatt taacaaatca tagctatcct
     6181 atctcaaaat taggaacgta tctaactaca gaactgctat agctctgtgc ggttttcaat
     6241 agccctcaac agggtcactt catctgcata ttcaatatca ctgcctaccg ctaagccacg
     6301 agctagtcta gttactttga ttccagcagg tttaaggaca cgtgagatat acatggatgt
     6361 agcctctcca tctgctgttg cattagtcgc cacaatcacc tctgtcacct cattttccat
     6421 caagcgggtc agcagagttt tcaggttaat atcgtctggc ccaatcccgt tcatagggga
     6481 aatcaagcca tgcaatacat ggtagagacc gtgatactct tggatatttt ccaaggctgt
     6541 aacatctcga ctatcctcta caatcaaaat agtagactgg tctcgcgttg agtcctggca
     6601 aatggcacag gggtcctgat ctgtcaaatt accacaaatg gagcaataag ataggtctcg
     6661 cttggctgcc aataaatttt ttgcaaactc attaacgaca tcatcctcca tgccaatagt
     6721 ataaaaagct aaacgggtag ccgtttttat accgatacct ggcaatttag aatagctatc
     6781 aattaacttg gcaataggtg tagggtaaag cataagcctc cttaattcat tgggtgaagt
     6841 gattgatata gattgataat ttcttgagta attgtcttag tagttgttga actttgattg
     6901 gtcaagtttg gtaggacaac tgctaccgca atttgaggat tatcactagg cgcataagca
     6961 acaacgttgg tattgaccgc agtaaccaca tcaccagaag ctgtcgttgt gtaggtttcg
     7021 gctgtaccag tcttggcact gatggtcacg gccgcaccac gaccgatagc actacctgta
     7081 ttaaagcgcc cactaccatt gacaacttgg tagaaacctt gacgaagaag agacatatcc
     7141 tcagcggaaa tattaacctg attcatctcc ttaccagata cggtttcaat taattccccc
     7201 aaaccacctt gggcattatt cccataaatt ccctcaacga ggtgtggaga aatccgtgtc
     7261 ccgttattcg caatggtcgc tgcatactgt gccatctgca tcggtgtata gttatcaaac
     7321 tgtccaaaag cgttggtaat aaagttggct gtcgtatact tatctggtaa aaatcctgta
     7381 gattcgagtg gaaggtcaat acctgtagaa gcaccaagac catattctgc aaaagtcgaa
     7441 cgaagcttag tcatggcgtt tttcaatgta tcaccatcat tcaaatacat gttagctgag
     7501 taggtctgac cgagtagtcc caaagcaatc ttaaccatat agatgttaga agaatattcc
     7561 agagcctctg tcgcatcaat attaaaacta ccataggccc cataccaaga tgtaattggt
     7621 gctgaacctg caaaatagat tggttcatct aattgaatct gattaccact gataactcca
     7681 ttttcccaac cagagctgat tgtcgctcct ttgacgatgg aacctggtgt aaagacactt
     7741 gtaatcgtac caagagcatc ctccgtaata gtacttgagc ctttttcatg actatatcct
     7801 gacatagcaa gtactgcacc tgtagaagga tttaatgcaa ctgcataaac accttccgag
     7861 taaagcgcac taccagtagc tagctcactt tcaaagtatt ttctcaaaat agcattgacc
     7921 ccatcttgaa aggctaaatc aatagtcagt ttaatattat ttccctgctg tccttcttgg
     7981 attgtctcaa tactttcgat atttccattt ctatctaggt ttatttgttt tttctcacgt
     8041 tgaccttgca agacttcttc atattgtttc tcgaggtagg cagttcccac acggtcattt
     8101 gaagagtagc ccttggatag atagtattct gcatcttctg caggcaaacc agcctgttca
     8161 ctcgttactg taccgataat ggagctgagg gaagttggta aaatcgtccg ttgccagtta
     8221 ttggtcgttg aaatgcctgg aagttgactt tcattggcta cgatatgagc aacctgttca
     8281 gaagtcagag tatccgttac caaattaaca gtagagaagt aagtagctcc attcatctgc
     8341 ttgaagagtt caatcgcttt attgtcttgc tcagaatatt gtaatgcttc ggcagggata
     8401 ctatcaactg cattagaata aattgttgat tcgctcaagt agttaccatc ggaatcgtat
     8461 tttttatctt ttggcaaggc ctcgactacc tgacgataaa cctcttcatc tgccaaataa
     8521 taatcagcct tatcacgttg agtaattgtt acgtctgaaa cattgaccca ctgcaagagt
     8581 ttattagcta cttctttcat ttcctgagca gacatcttgt tgggacgtgt gaaggagacg
     8641 acctgctgaa tctcgttttc aaccaaaggt tttccctgcg catcgtaaat ctgcccgcga
     8701 atagagccat tgctgatgat tttctgactg gcagtagaca gcttattact ataaaaatca
     8761 tgattgatga tctgcatatc tgccagacgc aatatcaaaa ctaggaaaag gaaaatcacg
     8821 atagaaaata aaatatttaa acgaagggaa ataaaatttc catcatgttc caaaggcttg
     8881 ttctttcttt ttttcacttc aaagacctca ttctagaaat atccttctta ttataccaca
     8941 ggcaaaaaat attttttaga cagagcctcc ttttcagaaa atattttctt gtgatttctt
     9001 cacgagacaa gcttgcacta tctcccattg actgtgaaca gatgtttcca caaactgttg
     9061 atcatgggaa atcaagggga cacttccttc atattctttc aagaatgttt ccaaaacctc
     9121 aatagctgcc atatctagaa aatttgtcgg ttcatctagt atcaaaagat ttgcatccgc
     9181 caacaatacc ttagctaagg agagacgaac acgctcacca ccagacagat ttgctacctt
     9241 ctgctgagcc ttatcgcaag aaagtcctaa catacctaat aaattgagaa tcatcatcct
     9301 atcttgaaga gaggtacaac taacattttc aaaagcagtc ttttcttcat caaggtttat
     9361 caaatcctgt gcaaaataag caatttttaa cttaggatta taatagcctg ctaattcttt
     9421 cctaaataat tttcggacaa aactagtctt tccagaacca ttcgccccca ctaaggctaa
     9481 cttctctcca aacgtcatcc ctatctgagg gacatcaaat agatacactt catccatcaa
     9541 aacttgacca gcctgcaagc gaaagagact gtgaagaccc gtatccaagg cacctttcgc
     9601 ttccatcttt acccacgttt cctttctagg ttgttcaacc ttgtccaaac gctctatccg
     9661 tttttccaaa tgcttggcag tcttggccat agacttggct tgactatcat aggaccccat
     9721 catggcactg actttccact cagacgatat ttagaataaa tgtccacatt cacttctcaa
     9781 agttcgcgtc aacatctcag cgcagtggtt gattggcaga tttgttcgtt tcttacactc
     9841 caaatctgac ctaatcaact gtgcgggggt gggatgacga agtttttata attggttgac
     9901 ttctatccca ctccctctct tccccatctt ctgagccttg gcttgacgtt catgttgggc
     9961 ttttttcatt tgggcaactt ttttctgata ggcttcataa gactcctgct gtccttctcg
    10021 cctagcccgg cgactttcta caaacgcctc gtaatttcct agataaacct gaaccttttc
    10081 ttcttctaaa tgccaaatgt gactggctat ttgattgaga aaatgccgat catgactaac
    10141 aactagtaga ctacctcgat agcgtttgat ttgtttgatt agcttttcct ggtgttcttg
    10201 atccagattg gcagtcggtt catccatgat taacaactgt ggtctttggg caaaagcctc
    10261 ttgaatagac ttccaaacct gttccccacc tgaaagcgag ctcctttcct gtaactgggg
    10321 tacataggcc cagtctgctt ttacctcaat ctgaccggca aaatccctat ccagtcctag
    10381 taagattttc aaaaaagtag acttgccaac tccgttattt ccaaccaaac caaccttttg
    10441 accagctagc ttgaaggctc aactgcttgg gagcaggaaa gaactcgacc aagataaaaa
    10501 gagttcgtct tcccgccccc gcacagttga ttaggtcaga tttggagtgt gtgtgaagca
    10561 cgaacaaatc tgccaatcaa ccactgcgct gagatgttga cacgaaccct gagaagcgag
    10621 gttaagcttt ttgcccagcc tctacgtccc gcaaattcct tctctaatcg aatacatttt
    10681 attacttcca tatagacctc ttttcttcac aaaaagggcc ccaattctgc aaaatggaca
    10741 cagaaaaaag ccatgcaaaa catgaccatt ccctatggga aaagtcacac gaatagtaag
    10801 cccttatgag cagtcttatc ccttgtatag tcagttaatg taacctcacc acaagtttta
    10861 agagcaacta ataagtagct taattattca tgtaacttca cctcgtataa ttagtaattt
    10921 cagtataact tagtttgtat caattgtcaa atcttggtca agggagtcgc tgtatctggc
    10981 gatgtgtcat agacttggaa atccactcca actcctaaat cagcctgcgc caactgatta
    11041 accatggtca tgtgagccaa ttccttgata ttgttttcct ttgacaaatg ccctaggtaa
    11101 atctttttgg tccgatttcc caacgagcga atcatggcgt cagctccatc ttcattacaa
    11161 agatggccct tatctgacaa aatccgttgc ttcaaattcc atgaatatga acctgcgcgc
    11221 aaaatttcaa tatcatggtt cgattcaatc aaataagcat cggcattttc tacaattcca
    11281 accatgcggt cactgacata gcctgtatcc gtcaacataa caaaggattt gtcatccttc
    11341 ataaaacggt aaaattgcgg gcatgcagca tcatggctaa ctccaaatga ctcaatatct
    11401 aggtcgccaa aagtcttcat agcacccaat tcgaagatat gtttttgagc cacatcaatc
    11461 ttgcccaatt tgctctccat agcctgccag gtcaattcat tggcataaat gtccatgcca
    11521 tacttacgtg ctaaaacacc gataccatgg atgtgatcgc tatgttcatg cgttaccagg
    11581 atagcatcaa tatcctcagg ctttcgatca atttccgcca aaagactggt aatctttttc
    11641 cctgataagc cagcatccac taagattttc tttttatctg tttctaggta aaaacaattc
    11701 ccgctggacc ccgaagctaa aatactataa ttaaaacctt ttccgatcat attctcctat
    11761 tcttcctcat ctgaatcatc ccactcatct agtgcgatgt ccttgctata cggcaagaca
    11821 attgtaaacg ttgagccatg accatattcg cttttagccc agataaagcc cttatgttgt
    11881 tttacgattt cttttgcaat agctaaacca agacctgtac caccttgggc acgagatcgt
    11941 gccttgtcta cgcgataaaa tcggtcaaaa attctaggta aatctgcttt cggaatcccc
    12001 agaccttcgt ctgatacgga tacaatcaac tggctatctg tggtcttcat gctgaaggta
    12061 attgtcccgc cgtctggcga atatttaatg gcattattta agatattatc taaaacctga
    12121 gtcatcttat ctgtatcaat ctcaacccaa ataggactga tttgatagtc acgaacaatc
    12181 gtatagacct tatctgaatc agcatttttc atttggtcaa aacggttaag aataaaggtc
    12241 acaaaagcag taaagtttat cagttcgaca tctatctgac caacttgatt atcaatgcgc
    12301 gataagctaa ggagatctgt aatcatccgc atcatgcggt tggtctcatc taaggatacc
    12361 ttaacaaaac tcggcgccac agactccgtc agcgctccct catccagagc ctctagatag
    12421 gatttaacgg aagtcaatgg cgtacgcaat tcatgactca catttgacac aaacagccta
    12481 cgttcccgtt cttccttggc ctgctcggtc atatcatgca agacgacaac caggccagaa
    12541 atcagaccac tctcactacg aatcgttgca aaattagcgc gtaaagtcag aaattcttcg
    12601 ttttcattgg tgtgatcaat cacaatctca ggtgtctgag ccaacaaatc tctcaatgta
    12661 taacggtctg ataaatctaa aacctctagc aggtgatact gctcctgctt tgggttagat
    12721 aaccccaact gtttctgggc catatcgtta atcatgatga tccgtccgat acggtctgta
    12781 gcaataacac cgtcactcat ataggacagg atggacgtca gacgtgtctt ttcttgttct
    12841 aaattatcat gagttaagcg gatgacctcg gacaaatcat tgagggaatt agccatgtct
    12901 gtgatttcag gacttcccct catgtctaac agatctgcat aattcccctc aattaaggca
    12961 ttgacttttt gattgagttg tttaatttgc ttattatccc aatagttttc aatcaaaagg
    13021 accgtcagag caattagaaa gccaatcaaa atgacaacaa accaaaattc tgccgtggtc
    13081 attaaataac gtaattgatt aatcatttcc ttttataaaa tatcccactc cgcgacgagt
    13141 aagaatgtat tctggtctgc ttggtgtatc ttcaattttc tcacgcagac gacgaatcgt
    13201 tacatccacc gtgcggacat caccaaagta atcataaccc caaactgttt ccaatagatg
    13261 ttctcgagtc ataacctgac ctaagtgttt ggccagatgg tggagcaatt caaactcacg
    13321 atgggtcagc tccagctctt taccatgctt cttagcaaca aacgcatcag gcagaatgac
    13381 caaatcgcca atcaccaact ctggagttcc tgttgactca atatttgtct gcgtctctgc
    13441 caattcacta cgacgaagaa gagccttaac acgcgcctgt aattcgcgat tagagaaggg
    13501 cttggtcaca taatcatccg cccctatttc aagcccgata accttatcaa attcgctatc
    13561 tttagcagat aacatcaaga ttggaacatt gctg
//
LOCUS       DBNFCK010000046        13243 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.046, whole genome shotgun sequence.
ACCESSION   DBNFCK010000046 DBNFCK010000000
VERSION     DBNFCK010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13243
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.046"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(11..586)
                     /locus_tag="AC1PIW_001059"
     CDS             complement(11..586)
                     /locus_tag="AC1PIW_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIP6606006.1"
                     /translation="MNLIIIGAQASGKMTVGQEVAKLTGMTLFHNHDSIDFSLRFIPE
                     FSEDMFDLNTRITFAVYDVFARSGRPLIGTALINFKNLIEVQFLTTVQTIFHHHGQEI
                     LFVELETALEERLRRNRTENRLTHKPLKRHIEVSEAEILSTADTCRYTSLGIPEGIQH
                     YLKINNTHLSANDVAQLIIQKMEELEQEQTK"
     gene            complement(583..972)
                     /locus_tag="AC1PIW_001060"
     CDS             complement(583..972)
                     /locus_tag="AC1PIW_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIP6606007.1"
                     /translation="MLHHVEIYVSDLETSRAFYDFLLTKLGYSLYQEWDKGLSYKKAE
                     QYLVFVQTPEDFLEVGYHRCRTGLNHLAFHAGTPDEIDQWRKEFLTRRVKLLYDDRYP
                     HAGGPDHYALYLEDPDGIKIELVGEGV"
     gene            complement(1214..1600)
                     /locus_tag="AC1PIW_001061"
     CDS             complement(1214..1600)
                     /locus_tag="AC1PIW_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIP6606008.1"
                     /translation="MAKKKVNRKKQLKKQLADLKRAGRVGLERAAEVVETVAHKAEAV
                     VERTVEQVKEVVAEVTSSATSLEDFLALPELEGIAAARLETFYEAGIQSVADFANHTE
                     KELLALKGIGPATIKQLKEKGIELKA"
     gene            complement(1615..1869)
                     /locus_tag="AC1PIW_001062"
     CDS             complement(1615..1869)
                     /locus_tag="AC1PIW_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HIP6606009.1"
                     /translation="MTKEQEFLKEFEAWVNTQVMVNEMAVEESRRVLEEDKDERAADA
                     YIRYESKLDAYRFIQGKFANYHAGKGFHDLPDELFGQRHY"
     gene            complement(1979..2980)
                     /locus_tag="AC1PIW_001063"
     CDS             complement(1979..2980)
                     /locus_tag="AC1PIW_001063"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HIP6606010.1"
                     /translation="MIEVKTFDEKAKLYCLENKNGMQVTLTDFGARVVEVLLPVEENG
                     GLRNVSLAAKSDDDYRKTDLYPGSTIVPVAGRISGAQAEIEGTSYHFTENEPGRTLHG
                     GVDTANEQYWDVELDHERNQVTFGMVLKDGFNGFPGDVRVKAIYCLTDKNELTVDYQA
                     VSDKDTIFNPTNHIYFNLTGDFQRSVAEHRIKIAANHYAPLGEDNLPTGVLEDVTGTP
                     FDFRDFAPFAQGFDSQYPQNVLVKGYDHPWLLEEVDIPVEVLSPDGKIGLSVKTNQPA
                     VVIYTYNFPVEALACYHGVFSLECQALPNACNVDGFGSILLEQGEEFLSKTTYRFTW"
     gene            complement(3196..3597)
                     /locus_tag="AC1PIW_001064"
     CDS             complement(3196..3597)
                     /locus_tag="AC1PIW_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIP6606011.1"
                     /translation="MVRSLVLVSHGIFCEELKKSTEMIMGPQEDIYTVALLPEEGPED
                     FQKKFEETIAGLEDFVVFADLLGGTPANVVSRKLIEGGQFDLYAGMNMPMVIGFLNGV
                     LLGEAVDYVEFGTSNLVHVNSLLTSDEDDDE"
     gene            complement(3597..4415)
                     /locus_tag="AC1PIW_001065"
     CDS             complement(3597..4415)
                     /locus_tag="AC1PIW_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HIP6606012.1"
                     /translation="MTKSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKLHTQFFNTSPFFHTIITGIDLALEESDGVASKDAVNGVKT
                     GLMGPFAPIGDSIFGSLVPAIMGTVAATMGKEGSPVGIFLWVAVAIAYDIFRWKQLEV
                     AYKEGTKLITTMRDRLTALVDAASVMGVFMMGALIATMINFEVTWAPAIGEKVIDIQD
                     ILNTIFPRLVPAIFTGFVFWLLGRRGMTSTKAILIIIVLALGFSAIGHFLFGMV"
     gene            complement(4402..5304)
                     /locus_tag="AC1PIW_001066"
     CDS             complement(4402..5304)
                     /locus_tag="AC1PIW_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HIP6606013.1"
                     /translation="MMQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGN
                     MEVGLLIGASLQLVVLGVGTFGGASRIDATSGAVLATAFSISQGLDPEIAISTIGVPV
                     AGLLVYTDILGRFTTTFFAHRVDAAIERFDYAGIERNYLLGAIPWALSRALPVFLALA
                     FGGGLVETLVTTIELPEYKWIAAGLTLAARMLPGLGFAILLHYLPLKRNLHYLAVGFA
                     LTAMLTVLYGNVSALGGAVAGIVGTLPEDAGVSFVNNFKGLSMIGIAIVGAFLSVIHF
                     KNSQKVTVVAPSNSESGEIEDDEI"
     gene            complement(5343..5828)
                     /locus_tag="AC1PIW_001067"
     CDS             complement(5343..5828)
                     /locus_tag="AC1PIW_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HIP6606014.1"
                     /translation="MAIIATRIDGRLIHGQVANLWTTKLNIGRIMVIDDAVAQNDIEK
                     QGLKLACPPGVKLSILPIEKAANNIKEGKYDSQRLLIVARRPENFLRLVEYGVELAEL
                     NVGNMSQTPETRSVTRSINVVDKDIADFDALAAKGVKLFAQMVPGDSPKDFMPLLDKV
                     R"
     gene            complement(5882..7654)
                     /locus_tag="AC1PIW_001068"
     CDS             complement(5882..7654)
                     /locus_tag="AC1PIW_001068"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 35 protein"
                     /protein_id="HIP6606015.1"
                     /translation="MKEFYIGDQFYLDGEPFKILSGAIHYFRVHPDDWYHSLYNLKAL
                     GFNTVETYVPWNMHEPRKGEFCYEGILDIERFLKLAQELGLYAIVRPSPYICAEWEWG
                     GLPAWLMKEELRVRSSDSVYLQHLDEYYVSLIPKLAKLQLAQGGNVLMFQVENEYGSY
                     GEEKAYLRAVAGLMRKHGLTAPLFTSDGSWRATLRAGTLIEDDVFVTGNFGSKARENF
                     ANMTAFFNEHQKNWPLMCMEFWDGWFNRWGDEIIRREPEEMVDSVMECIELGSLNLYM
                     FHGGTNFGFMNGCSARGQIDLPQVTSYDYDAILDEAGNPTKKFYILQQRLKEVYPELE
                     YAEPLVKEAKAFSDVSLHDKVSLFATLENVSDCVKGFYPKNMEELDQSTGYILYRTEL
                     ERDKTEAERFRVVDARDRIQIYADGKFVATQYQTEIGDDVELDFKDDKLTLDILVENM
                     GRVNYGHKLTAPTQSKGIGRGAMADLHFIGHWETYPLHLESVEDLDFSKGWEEGQAAF
                     YRYQFELDELADTYLDMTGFGKGVVFVNNVNIGRFWEKGPILYLYIPKGYLKKGANEI
                     VVFETEGKYREKIHFSQRPVIKDL"
     gene            7861..8571
                     /locus_tag="AC1PIW_001069"
     CDS             7861..8571
                     /locus_tag="AC1PIW_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIP6606016.1"
                     /translation="MKVPKYQLIQNDLRQQIVSGKFENGDKFYTESELTKLFNVSSIT
                     VIRAVNELVKDGYLVRQQGKGTFVSRSRKGRLVEFSDIEIFPMDKDKVTVLSCEKGNK
                     PDILEKLNLDKNEFYYKIVRVRAAEDTPYIFHNSYIPQRYIQNPDAPLEHYQSIYQRF
                     KLDYNIHMSEEPFVETNEIVSPCPKEVATHLKLKATEPAVLQNKTTTNSTSGEVMEYT
                     ETYKHWKYYKFEITANHR"
     gene            complement(8711..9442)
                     /locus_tag="AC1PIW_001070"
     CDS             complement(8711..9442)
                     /locus_tag="AC1PIW_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIP6606017.1"
                     /translation="MVSQTVEKPLYLQLVDELEVAIRERMAPNDKLFSERELTQVYGV
                     SRITVRLALQELEKRGLVYKKHGKGTYVSEISDTAVDLSQAYSFTEQMKKIGKVPRTS
                     ILSFELVKASDYIAQHLQLSQGEEVFEVERLRLADEIPMMLERTYVPASVFPGLAAQR
                     MKSTPLYEIFSEDYHQVIRLAEEEFYASIALDNEAKILGIPSNSPVLHLVRKTYNDKN
                     RIIEFTFSIARADQFRYKITHQRGY"
     gene            complement(9542..9859)
                     /locus_tag="AC1PIW_001071"
     CDS             complement(9542..9859)
                     /locus_tag="AC1PIW_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HIP6606018.1"
                     /translation="MAFEQTNGRYASFGVVTSLPGEVIDSFWYVIDHYLKGVIPLKSV
                     IRFSIKNRRGKITLVFSQEGYKNVLAVDLSSRFDPFYPSTILVMDKQGKETITLPDEV
                     TLL"
     gene            complement(9917..10450)
                     /gene="ntdP"
                     /locus_tag="AC1PIW_001072"
     CDS             complement(9917..10450)
                     /gene="ntdP"
                     /locus_tag="AC1PIW_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017110 - nucleoside diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside tri-diphosphate phosphatase"
                     /protein_id="HIP6606019.1"
                     /translation="MKLPKEGDFITIQSYKHDGEIHRTWRDTMVLKTTENAIIGVNNH
                     TLVTENDGRRWVTREPAIVYFHKKYWFNIIAMIRENGVSYYCNLASPYVLDNEALKYI
                     DYDLDVKVFADGEKRLLDVDEYERHRKAMKYSDDIDFILKENVKILVDWINNQRGPFS
                     PAYVNIWYKRYLELRSR"
     gene            complement(10526..11302)
                     /gene="recX"
                     /locus_tag="AC1PIW_001073"
     CDS             complement(10526..11302)
                     /gene="recX"
                     /locus_tag="AC1PIW_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HIP6606020.1"
                     /translation="MRITKIEKKKRLYLLEVDGQDSLYITEDTIVRFMLSKGKEITEQ
                     EFRELRDFAQFSYGKNLALYYLSFKQRTKKEVSNYLKKYEIEENNIVKIVTVLEEEKW
                     LDDGNYVDSYVRQNALNGDKGPAMIRQKLMQKGIPKTLIDKRLAEEDFSELAGKIGEK
                     LVGRYQRKLPLRALQDKVVQGLMGKGFSYEAAKQSLGQLELVADEENEDDLIAKELDK
                     QYRKYSRKYEGYELKQRLIQALARKGYDFDRIQAVLRDYL"
     gene            11340..12692
                     /gene="rlmD"
                     /locus_tag="AC1PIW_001074"
     CDS             11340..12692
                     /gene="rlmD"
                     /locus_tag="AC1PIW_001074"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIP6606021.1"
                     /translation="MNLQVNQRIPLKIKRMGINGEGIGFYQKTLVFVPGVLKGEEIFC
                     QITSVKRNFVEAKLLKINKESKFRVKPACDIYRACGGCQIMHLRYDKQLDFKQDLLQQ
                     ALKKFKPAGYENYTIYPTIGMDKPSHYRAKLQFQTRSFKDQVKAGLYAENSHRLIGIT
                     NCYVQDAETQEIINGIAKLLTKHRIPIYNERKQHGIRTVMVRKARKTGQVQVIFITSC
                     QVNLIKLIDELTSQFPSIITIALNWNRQKTSDIYGEKTEILWGEQAIDEAVLDYEFSL
                     SPRAFYQLNPQQTEILYGEAVKALDISGDEDLIDAYCGVGTIGFAFAKKVKSVRGMDI
                     IPEAIEDAKANAKRMGLSNTHYEAGKAEEIIPKWYQEGYRADALVVDPPRTGLDEKLL
                     KTILNYKPAKMVYVSCNVSTLARDLVELSKAYKVEYIQSVDMFPHTARTEAVVKLSKK
                     "
     gene            12977..13180
                     /locus_tag="AC1PIW_001075"
                     /pseudo
     CDS             12977..13180
                     /locus_tag="AC1PIW_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943028.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4140 a         3077 c         2202 g         3824 t
ORIGIN      
        1 tttgggctgg ctatttcgtt tgttcttgct caagttcctc cattttttgg ataattagtt
       61 gagctacgtc attggcagaa agatgagtat tattaatttt gagataatgc tgaatgccct
      121 ctggaatgcc taacgaggta tatcgacatg tatcagctgt agagagaatc tcagcctctg
      181 aaacttcaat gtgtcgtttc aaaggtttat gggtaagtcg attttctgtc cgattacgcc
      241 gcaaacgttc ttccaaagcg gtttccaact caacaaataa aatttcctgg ccatgatgat
      301 gaaaaatcgt ctgaactgtt gtaaggaatt gaacctctat gagattttta aaattaatca
      361 aggcagttcc gattagtgga cggcctgacc tagcaaacac atcataaaca gcaaatgtaa
      421 tacgtgtatt gaggtcaaac atatcctcag aaaattcagg aataaaacgt aggctaaaat
      481 caatcgaatc gtgattatga aaaagggtca tccctgtcaa tttcgcaact tcctgcccaa
      541 cggtcatctt gccagaagct tgtgctccaa taataattag attcataccc cctcccccac
      601 caactctatc tttatcccat ctggatcttc caaataaagg gcatagtggt ctggtcctcc
      661 tgcgtgggga tagcggtcgt cgtagagaag tttgactcgt ctggtcaaaa attccttccg
      721 ccattggtca atctcatcag gtgtccctgc atgaaaagct agatgattga gacctgtgcg
      781 gcaacggtgg tagcctacct ctaggaagtc ctctggcgtt tggacaaaga ccaggtattg
      841 ctctgctttt ttataagaca gccctttatc ccactcctga tagagcgagt agcccagctt
      901 ggtcaggaga aagtcgtaaa atgcacgcga agtttccaag tcagaaacgt aaatttcaac
      961 atgatgtaac atatcttctc ctttccaaat gaaaatcccc gcctagactt gctagacagg
     1021 gacgaagtta ttccgcggta ccacccttgt tcagatacat tatctgcact ttgcttattc
     1081 gattcacatc atcagcaacc agttttgaca tttgtgcgga tttctcagta ccaccgcttc
     1141 ctgtgacaaa tggggctcaa aacacctctg aatggcttta ttgtaacaaa ttgtaccttg
     1201 aaaagcaagg gatttaagct ttcagctcaa tccctttttc tttcaactgc ttgatagttg
     1261 caggaccgat gcctttaaga gccaagagct ctttttctgt atggtttgca aaatcagcca
     1321 cagattgaat acctgcttcg tagaaggttt ccaaacgtgc tgccgcaatg ccttccaact
     1381 caggaagcgc caaaaagtct tccaatgaag tcgctgaaga agtcacctct gctacaactt
     1441 ctttcacttg ttccacagta cgctctacta cagcctcagc cttgtgagca actgtctcta
     1501 ctacctctgc agctctttca agacctacac gacctgcacg tttcaagtct gctaattgtt
     1561 ttttcaattg tttttttcga tttacttttt tctttgccat gttaactcct ctccttaata
     1621 atgcctttga ccaaacaact catctggcaa atcatgaaaa cctttgccag catggtagtt
     1681 ggcaaatttc ccttgaatga atcgataggc atccagcttg ctctcataac gaatataagc
     1741 atccgctgca cgctcatcct tgtcttcttc caagacacga cgactttctt caaccgccat
     1801 ctcgtttacc atgacctgag tgttgaccca agcctcaaat tcttttaaaa attcttgttc
     1861 ttttgtcatt attttcctct ttcgcctgct tcgattgaaa cacaccggtt tattatacca
     1921 tattttctag aaaacacaag aaaaccctaa ctgtaacaca gttagagtcc ccattttttt
     1981 accaagtaaa acgataggtc gttttagaca aaaattcttc tccttgttct aacaaaatcg
     2041 aaccaaatcc atcgacattg catgcatttg gcaaggcctg acactctaaa ctaaagacac
     2101 catggtaaca ggccaaagct tcaacaggaa aattatatgt ataaataacg accgcaggct
     2161 ggttggtttt gacactgagt ccaattttcc catctggact caaaacttct accggaatat
     2221 ccacctcttc caataaccat gggtggtcat agccttttac caacacattt tgaggatatt
     2281 ggctatcaaa tccctgagca aaaggcgcaa aatctctaaa atcaaacggc gtccctgtca
     2341 catcttctaa gacacccgtt ggcaggttat cctccccaag cggagcatag tgattggcag
     2401 caattttaat acggtgctcg gcaactgacc gttgaaaatc accagtcaag ttgaaataga
     2461 tatggttggt cggattaaaa atcgtatcct tatccgatac agcctggtag tcaactgtca
     2521 attcattttt atccgtcaaa caataaatag ccttgaccct cacatcacca ggaaagccat
     2581 taaaaccatc ttttaggacc ataccaaagg tcacttgatt tctctcatgg tctagctcaa
     2641 cgtcccagta ctgctcattt gccgtgtcaa ccccaccgtg tagagtccta ccaggttcat
     2701 tttctgtgaa atggtagctt gttccctcta tctcagcttg ggcacctgaa atacgccccg
     2761 ctactgggac aatagttgaa ccagggtaca aatctgtctt acggtaatca tcatccgact
     2821 tggcagctaa actgacattc cgtagaccgc cattttcttc tactggcaaa agcacttcca
     2881 ccactctggc accaaaatcc gtcaaggtta cctgcatccc attcttgttt tccaagcaat
     2941 acagttttgc tttttcatca aatgttttta cttcaatcat ttcttttcct cacaagccaa
     3001 atgagggagt cactattgca acttcctcag gatacttaaa gcctatcaac tatagaacat
     3061 tttataatta acgaaaatca aatccgtctg taacaatcag ctgagttagc ctcactctct
     3121 tatttctagg ctcgggctgt ctttggtcac tctttcattt caaggtgacc agtggaaaca
     3181 gtttcccaaa ctgtttcact catcgtcatc ttcatcactt gttaatagac tattgacatg
     3241 aactaggttg cttgtcccaa attctacata atccacagcc tctcccagca ggacgccgtt
     3301 caagaaaccg ataaccattg gcatattcat ccctgcataa aggtcaaact gcccaccttc
     3361 aatcaatttt cgtgatacga cgttggctgg tgtaccgccc aacaagtctg cgaagacaac
     3421 aaaatcttcc aatcctgcaa ttgtttcttc aaattttttc tggaagtctt ctggaccttc
     3481 ttctggtaat aaagctactg tgtagatgtc ctcttgagga cccataatca tttcagtaga
     3541 ttttttcaac tcctcgcaga agataccatg acttactaat actaaacttc taaccattat
     3601 accataccaa ataggaagtg accgattgct gagaagccaa gtgcaagcac aataataatc
     3661 aagatagctt tagtcgaagt catgccacga cgaccaagaa gccagaagac aaatcctgta
     3721 aagattgcag gtactaggcg tgggaagatg gtattcaaaa tgtcttgaat atcaatcact
     3781 ttttctccaa tagctggagc ccaagtcact tcaaagttaa tcatagttgc aatcaaggca
     3841 cccatcatga agacacccat tacagatgct gcatcaacga gagctgtcaa gcgatcgcgc
     3901 atggttgtaa tcaacttagt accttctttg taggcaactt ccaactgttt ccaacggaaa
     3961 atatcatagg caatggctac tgctacccaa agaaagatac caactggaga tccctcttta
     4021 cccatagtag cagccacagt ccccataatc gctggaacaa gagaaccaaa gattgagtca
     4081 ccgattggag caaatggtcc catcaaacct gtcttaacac cgttaaccgc atctttagaa
     4141 gcaacaccat cactttcttc aagtgccaag tcaatacctg tgatgatggt atggaagaat
     4201 ggtgaagtat tgaagaactg agtatgcaat ttcatcattt ccttcaactc aggtgtacca
     4261 tctccataca ttttacgcaa ttgtggcaaa atcatgtaga ggtagccaga tgcctgcatc
     4321 cgctcatagt tccaaccaag ttggaaagtg aacaagctac gtttgttgat ttgattaaaa
     4381 tcttcttttg ttaatttgta attagatttc gtcatcttca atttccccac tttctgaatt
     4441 tgatggagca accacagtta ctttttggct atttttgaag tgaatcactg aaagaaatgc
     4501 acccacaata gcgataccaa tcattgacaa acctttgaag ttattgacaa agcttacgcc
     4561 tgcatcttca ggaagagtgc caacgatacc agcaactgca ccacccaaag ctgatacgtt
     4621 accgtaaaga acagtcaaca tagctgtaag agcgaaacct accgccaagt agtgaaggtt
     4681 acgtttaagt ggaagatagt gaagtaagat tgcaaatcca agacctggaa gcatacgagc
     4741 tgcaagagtt aagcctgctg caatccattt gtattcaggc aactcaatcg tagtgacaag
     4801 agtttctaca agaccaccac caaaagcaag ggcaagaaaa actggaagtg cacgagaaag
     4861 tgcccaagga attgcaccaa gaaggtagtt gcgttcaata ccagcatagt caaaacgttc
     4921 gattgctgca tctacacgat gagcaaagaa agtagtggtg aaacgaccta aaatatctgt
     4981 ataaaccaac aatcctgcaa ctggtacgcc aatagtggaa atggcaattt ctggatctaa
     5041 accttgtgaa attgagaaag ctgtcgcaag aaccgcacca gatgttgcat caatacgtga
     5101 cgcaccaccg aaagtaccaa ctcccaagac aactagctgc aaacttgcac caattagcaa
     5161 gccaacttcc atgttcccca tgattaaacc agtgataaac cctgcaaaga ctggtgaacc
     5221 tgctgacgat acaattgtca gctcgtcaca aatctggtaa gcagagtaga gcgttagaag
     5281 taatatttgc caccattgca taatagtgat cctcctttaa atttttcaaa aatatccgaa
     5341 tgttatctaa ccttatccaa taatggcata aagtcttttg gtgaatcgcc aggtaccatt
     5401 tgtgcaaata attttacacc cttagccgcc aaggcatcaa aatctgcaat atccttatct
     5461 acaacgttaa tagaacgagt tacagaacga gtttccggag tttgagacat attccctacg
     5521 ttcaattctg ccaattccac tccatattca actaaacgta ggaagttttc aggacgacgg
     5581 gcaacaatca aaagacgttg gctatcgtac ttcccttcct taatgttatt agcagccttt
     5641 tcgatcggta aaatcgataa ttttacgcct ggaggacatg ctaatttcag tccttgtttt
     5701 tcaatgtcat tttgagcaac agcatcatcg ataaccatga tacgaccgat gttcaatttc
     5761 gtcgtccata agttagcaac ctgaccgtgg atcaaacgac catcaatacg cgtagcaata
     5821 attgccatac agtgttctcc ttttcttcat aaatcttttg agcagaaacc gattaaaatt
     5881 tctacaagtc ttttataacg ggtctttgtg aaaaatgaat cttctctcta tattttcctt
     5941 ctgtctcgaa gacaactatt tcattcgctc ctttctttaa gtatcctttt ggaatataga
     6001 gatagaggat tggtcctttt tcccaaaaac gtccaatatt tacattatta acgaaaacaa
     6061 ctcccttacc aaaacctgtc atgtctaagt aagtatccgc aagttcatcc agttcaaact
     6121 gataacgata gaaagcagct tgaccttcct cccagccctt actaaaatcc aggtcctcaa
     6181 ctgactcaag atgcaaagga taggtttccc aatgaccaat aaaatgtaaa tcagccatcg
     6241 cacctcgacc aatcccttta gactgggtag gagccgttag tttgtgccca taattgacac
     6301 gtcccatatt ttctaccaaa atatctaatg ttagcttgtc atctttaaaa tccaattcaa
     6361 catcgtcacc aatctcagtc tgatactgag ttgcaacgaa ttttccatct gcataaattt
     6421 ggatacggtc acgcgcatca accactcgga aacgttctgc ttctgtctta tcccgctcca
     6481 actctgttct atataaaata taacctgttg actgatcaag ttcttccata ttcttaggat
     6541 aaaacccttt tacgcaatcg cttacatttt ctagagtagc aaacagactc accttatcat
     6601 gaagcgacac atctgaaaaa gcttttgcct cttttacaag cggttctgca tactctaatt
     6661 caggatagac ttctttcaaa cgttgttgca aaatataaaa cttcttagta ggatttccag
     6721 cttcatccaa tatagcatca taatcataag aagttacctg aggcaaatca atttgaccac
     6781 gagcagagca accattcatg aaaccaaaat ttgttccacc atggaacatg tacagattca
     6841 aggagcccaa ttcgatacat tccataactg aatcaaccat ttcctccggc tctctacgaa
     6901 taatttcatc gccccaacga ttgaaccaac catcccaaaa ttccatacac atgagtggcc
     6961 aattcttctg atgttcatta aagaatgccg tcatatttgc aaaattctct ctagctttag
     7021 aaccaaagtt tcctgttaca aatacatcgt cctctatcag tgttcctgca cgtaatgttg
     7081 ctcgccaaga accgtccgag gtaaacaagg gagcagttag accatgttta cgcatcaagc
     7141 cagccaccgc tcttaaatat gccttctcct caccataaga tccatactca ttttccactt
     7201 ggaacataag tacatttcca ccctgtgcga gttggagctt ggccaattta ggaataagag
     7261 aaacgtaata ttcatctaag tgctgcaagt acaccgaatc actcgaacgt actctaagct
     7321 cttccttcat caaccaagca gggaggccac cccattccca ctcagcacaa atataggggg
     7381 acggtcgtac aattgcataa agccctaatt cttgagccaa tttcaaaaat cgctcaatat
     7441 ccaatatacc ctcgtaacaa aactcaccct ttcgaggctc atgcatattc caagggacat
     7501 aggtttcaac cgtattaaaa ccaagggcct ttaagttata caaagaatgg taccaatcat
     7561 caggatggac acgaaaataa tgaatcgcgc ctgataaaat cttaaagggt tcaccgtcca
     7621 aataaaattg gtctccaata taaaattctt tcatttcttc ctccgatgca accgcttacc
     7681 tttctattaa tatactaaca cttctttgtg gttatgtcca gtacttttta aaaatttttt
     7741 taccatttct tatcaccttt ataaagtgaa aaagtatatt tttgtgggta ttttagctat
     7801 attttttgat tttcatcaac taatttagta taataagttg tggaaaggaa ggaaataagt
     7861 atgaaagtac cgaagtacca acttatacaa aacgacttac gccaacagat tgtttctggc
     7921 aaatttgaaa atggcgacaa attttacaca gaatctgaat taacaaaact atttaatgtt
     7981 agctccatta ccgttatccg tgcagtaaac gaactagtta aagatggtta cctcgttcgc
     8041 caacaaggta aaggaacctt tgtatctcgt tcacgtaaag gacgtcttgt agaattttct
     8101 gatattgaga tttttccgat ggataaggat aaggttactg ttctctcgtg cgaaaaaggc
     8161 aataaacccg atatccttga aaaactaaat cttgacaaga atgagtttta ctataaaatt
     8221 gtacgcgtcc gcgcagcaga agacactcca tacatcttcc acaattcata catccctcaa
     8281 cgatatatcc agaatcccga tgctccactc gagcactatc aatcaatcta tcaacgcttc
     8341 aaactggact acaatatcca catgtccgag gaaccatttg tagaaacgaa tgaaattgtc
     8401 agcccatgtc caaaagaggt tgccacccat ttgaaattaa aagcgactga accagctgta
     8461 ctacaaaaca aaacaactac taacagtact agcggtgaag tgatggaata tactgaaact
     8521 tacaagcact ggaagtatta caaatttgaa attacagcca accatcgcta aaaaaacgca
     8581 gactaacgtc cgcgtttttt tcatacccaa taacaatcaa aagtagccta ggaaacgaag
     8641 ccgaagatag aactggattt catcaaggct cttgacaacg aataattttg attttcgaag
     8701 agtattgatt ctaatatccc cgttgatggg taatcttgta acgaaactgg tccgctctag
     8761 caatactaaa cgtaaactcg ataattctat tcttatcatt atacgttttt cttaccaaat
     8821 gaagcactgg actattgctt ggaattccga gtatcttagc ttcattatcc aaagcaatac
     8881 ttgcatagaa ctcttcttct gccaaacgaa tcacctgatg ataatcctct gaaaaaattt
     8941 catacaaggg agttgacttc attcgctgag cagctaatcc aggaaatacc gaagcaggaa
     9001 catatgtcct ttccagcatc atcggaatct catcggcaag tctcaatcgt tcaacttcaa
     9061 acacttcctc accttgacta agttgtaaat gctgcgcaat atagtctgac gccttaacca
     9121 attcaaagga taaaatagat gtcctcggta ctttcccaat ttttttcatt tgctcagtaa
     9181 aactataggc ctgagacaaa tcaactgcag tatctgaaat ctcagataca taggttccct
     9241 taccatgctt cttatagact aagccccgtt tttccaattc ttgcaaagcc aaacgaactg
     9301 taatacgact gacaccataa acctgtgtca actcacgctc tgaaaataac ttgtcattcg
     9361 gagccatcct ctcacgaatt gcaacttcta attcatcaac caactgcaaa tagagaggtt
     9421 tttcaacagt ttgactaacc atagactcct cctttctaac ccacataagc ttacaaatct
     9481 attttacacc aaatttcata tattaacaat atcttgggtg ataaaatacc agctcttcaa
     9541 gctaaagcaa ggttacctca tctggcaaag taatggtctc ttttccctgc ttgtccatta
     9601 ccaagattgt tgagggataa aaaggatcaa aacgactact tagatctaca gccagtacat
     9661 ttttataacc ttcctgcgaa aagaccagcg taatcttacc acgacgattt ttgatagaaa
     9721 aacggattac actttttaat ggaataaccc cttttaagta gtgatcaata acataccaga
     9781 aactatcaat cacttcccca ggaagcgaag tcacaactcc aaaacttgca tagcgcccat
     9841 ttgtttgttc aaaggccata agcccctcct ttacaataag aaaaaagcct gtaaaacagg
     9901 attttctttt ctaactttat ctacttctca gttctaaata gcgtttgtac cagatattaa
     9961 cgtaggctgg agagaatggt ccgcgctggt tattaatcca atcaaccagt atcttgacat
    10021 tttccttcaa aataaaatca atatcatccg aatacttcat cgccttacga tgacgctcat
    10081 attcatccac atccagcagt ctcttttcac catcagcaaa gaccttaaca tctaggtcat
    10141 aatcaatgta tttgagtgcc tcattgtcga gaacatatgg actagcaagg ttacaataat
    10201 aggaaactcc gttttcacga atcattgcaa tgatattgaa ccagtatttt ttatgaaaat
    10261 aaacgattgc tggctctctt gttacccaac gcctaccatc attttcagtc actaaggtat
    10321 gattattgac tccaataata gcattttccg ttgtttttaa taccatggtg tcacgccaag
    10381 tgcgatgaat ttcaccgtca tgcttataac tttgaattgt aataaagtcg ccttcctttg
    10441 gtaatttcac tttgttacca actttctaca attacaattt atcttgacta ttatatcata
    10501 tttttctaac ctaggctaaa ttttttcaca gataatcacg aaggacagcc tgaatccgat
    10561 caaagtcata accctttcga gccaaagcct gtatcaacct ttgcttcaat tcatagcctt
    10621 catatttacg actatatttt ctatactgct tatccaattc cttggcgatt aaatcatctt
    10681 cattttcctc atcagccaca agttccaact gacccaggct ttgtttggcc gcctcataag
    10741 aaaaaccctt acccatcaat ccctgtacaa ctttatcttg caaagctcga agaggcaatt
    10801 ttctttggta tctacccacc aatttctccc caatcttccc agcaagctct gaaaaatctt
    10861 cctcagccag tcttttatca atcagcgttt taggaattcc tttctgcatc aacttctgac
    10921 gaatcatagc tggtccctta tctccattta gagcattctg acgaacatag ctatcaacat
    10981 aattcccatc gtccagccat ttttcctctt ctaataccgt cacaatcttg acaatattgt
    11041 tttcttcaat ttcatatttt tttagataat tactaacttc ttttttggta cgttgcttga
    11101 aggaaaggta ataaagtgct agatttttcc cataagaaaa ctgagcaaaa tcgcgaagct
    11161 cacgaaactc ctgctccgtg atttcctttc ccttactaag catgaagcga acaattgtgt
    11221 cttccgtaat atataaacta tcctgaccat ccacttcaag taaataaaga cgtttcttct
    11281 tttcaatttt tgtaattctc attctttcat tatacaggat ttttcggtaa aataataata
    11341 tgaatctaca agtaaaccaa cgaatcccct taaaaatcaa acgaatgggc atcaacggag
    11401 aaggcatcgg attttatcaa aaaacgctag tctttgtccc tggtgtcctt aagggagaag
    11461 aaattttttg tcaaattacc tcagtcaaac gaaatttcgt tgaagcaaaa ttgttaaaaa
    11521 tcaacaagga atcaaaattc cgtgtcaagc cggcctgtga catctatcga gcctgtggtg
    11581 gttgtcaaat catgcacctc cgctatgata aacaattaga cttcaaacaa gatctcttgc
    11641 agcaggccct aaaaaagttc aaaccagctg gctacgaaaa ctatactatt tatccaacaa
    11701 tcggtatgga taagcctagc cactatcgtg ccaaattaca gttccagact cgatctttca
    11761 aagatcaagt caaggccggt ctctatgcgg aaaattccca tcgcttgatt ggaattacga
    11821 attgttacgt ccaagatgcc gaaacacaag agattatcaa cggaatcgca aagctactca
    11881 ccaagcaccg tattcctatt tataatgagc gcaagcaaca tggaatccgc acggtcatgg
    11941 tccgcaaggc tcgaaaaaca ggtcaagttc aagtgatatt tatcacctct tgtcaggtta
    12001 atcttattaa attaattgat gaactcacca gtcaatttcc aagtataatc actattgctc
    12061 taaactggaa tagacagaaa acgagtgata tttatggtga aaaaacagaa attttatggg
    12121 gagaacaagc tatcgatgaa gctgttttag attacgaatt ctcactatct ccacgggcat
    12181 tttaccaatt aaatccccaa caaacagaaa ttctctatgg cgaggccgtc aaggctttgg
    12241 atatttcagg agatgaagac ttgattgatg cctactgtgg cgtgggtaca atcggcttcg
    12301 cctttgctaa aaaggtcaaa tctgttcgag gtatggacat tattccagaa gccatcgagg
    12361 atgctaaagc aaacgctaag cgaatgggac tatccaatac ccactatgag gcaggtaaag
    12421 cagaagaaat cattccaaag tggtaccaag aaggctacag ggccgatgcc ttggtagttg
    12481 atcctccacg aactggactc gacgaaaagc tcctcaaaac cattttgaat tacaaaccag
    12541 ctaaaatggt ttatgtatcc tgcaacgtct ccactcttgc aagggacctt gttgagctaa
    12601 gcaaagccta caaggtagaa tacattcaat ctgttgacat gtttccccac acagcacgga
    12661 ccgaggcggt tgttaaacta agcaaaaaat aaaaggcact ataagactat gacgggtaaa
    12721 cccactatgg tcatatagag cctttttgag tgtagaaaaa agtcccctac gacctatact
    12781 gatacccatt taaaatcaaa aatagccacc taccacttaa agtagatatg gagcattttg
    12841 attttgttat tttttgagaa aaaaacagct acttgaaatc cacaatcctc aactcaaact
    12901 tataaattaa ggttttacat gttataagta aattagactt gctatttttg atttttattg
    12961 agtatgaaaa acaacaaaac tcacaatttc caacaccaaa tcagaaacaa ctaacaatct
    13021 catcaaagtc attaaaagaa atgcctttgg attcaggaaa atggaaacta gaaaaacgga
    13081 ttttgatgct ttgaacagtg aaaagctgag gagagctctt ttcagctcat ctcctaaaaa
    13141 caagaaagag agaacgaatt tcgtcctctc taggtgttag cttttcatct acccactaca
    13201 gttgacaaag agccaaaaaa aaagaacatt gctgctctct tac
//
LOCUS       DBNFCK010000047        13198 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.047, whole genome shotgun sequence.
ACCESSION   DBNFCK010000047 DBNFCK010000000
VERSION     DBNFCK010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13198
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.047"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            3..75
                     /locus_tag="AC1PIW_001076"
     tRNA            3..75
                     /locus_tag="AC1PIW_001076"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36..38,aa:Asn,seq:gtt)
     gene            98..169
                     /locus_tag="AC1PIW_001077"
     tRNA            98..169
                     /locus_tag="AC1PIW_001077"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:131..133,aa:Glu,seq:ttc)
     gene            288..2306
                     /gene="recG"
                     /locus_tag="AC1PIW_001078"
     CDS             288..2306
                     /gene="recG"
                     /locus_tag="AC1PIW_001078"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIP6606022.1"
                     /translation="MKRLCDELSVLPGIGPKSAEKFLKISIQNINDLLTYYPFRYEDF
                     ESKSIYDLQDGEKAVVVGEVVSPANVQYYGYKRNRLRFSMKQGEVVLAVSFFNQPYLA
                     DKIVLGQDIAVWGKWDKAKASLTGMKILAQVSDELQPVYHVAQGISQVNLVKAIKTAI
                     DQGYLHLLEENLPSVLRERYRLMNRREAVFAMHFPTNLEEYRQALRRMKFEELFYFQL
                     QLQMLKANNRDILNGLKIAYDADRLAMQIRQLPFVLTDAQSGALAEILSDMKSYGHMN
                     RLLQGDVGSGKTVVAGLAMFAAVTAGMQAAIMVPTEILAEQHFESLRQLFPELSIALL
                     TGGMKAAERRTALEAISSGQVDIIVGTHALIQESVTYHKLGLVVTDEQHRFGVKQRRL
                     FREKGDNPDVLMMTATPIPRTLAITAFGDMDVSIINQLPAGRKPIITRWVKHQQLPTV
                     LDWLERELEVGAQVYFISPLIEESEALDLKNAVDLQSDLQAHFGEQVTVDLLHGKMKN
                     DEKDAIMQAFKERKTNILVSTTVIEVGVNVPNATVMVIMDADRFGLSQLHQLRGRVGR
                     GHKQSYAVLVANPKTESGKERMKIMTETTDGFILAEADLKMRGSGEIFGTRQSGLPEF
                     QVANIIEDYPILEEARRVASQIVSVENWQEDPNWSILLANLKDREELD"
     gene            complement(2370..3329)
                     /locus_tag="AC1PIW_001079"
     CDS             complement(2370..3329)
                     /locus_tag="AC1PIW_001079"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HIP6606023.1"
                     /translation="MKKILVLHTGGTISMQTNHQGEVAANKTNPMTQIDSPLEETQVT
                     SLDFLNVPSPHIRLEHMMTLYQKIKEEQMNFDGFVITHGTDTLEETAYFLDTMAIPEK
                     PIVLTGAMRSSNELGSDGIYNYRTALRVATDDKSADKGVLVVMNDEIHAAKYVTKTYT
                     TNVSTFQTPTHGPLGLVTKREILYFKTAEPRVRFDLSTVTGTVPIIKAYADMDSILLD
                     SLTASSISGLVIEALGAGNLPPTILPAIKKLLDQKIPIILVSRCFNGIAEPVYAYQGG
                     GIQLEKDGILFVKELNAQKARLKLLIALNAGLEEQSLADYIQG"
     gene            3399..4764
                     /locus_tag="AC1PIW_001080"
                     /pseudo
     CDS             3399..4764
                     /locus_tag="AC1PIW_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002891795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
     gene            complement(4780..5232)
                     /locus_tag="AC1PIW_001081"
     CDS             complement(4780..5232)
                     /locus_tag="AC1PIW_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0050896 - response to stimulus [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIP6606024.1"
                     /translation="MTQSYKTILVAVDGSKGAELALHKAIHVALRNQARLIIAHVIDT
                     RALHNVVAFDASVYESLEREAELLLEEYKQEALNAGLTDVQIRIEFGNPKTLLAIDIP
                     KETGADLMLLGATGLNAFERLLIGSSSEYIMRHATIDLLIVRDGEKSL"
     gene            5387..6601
                     /locus_tag="AC1PIW_001082"
     CDS             5387..6601
                     /locus_tag="AC1PIW_001082"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIP6606025.1"
                     /translation="MKQFNKSTKLDDVAYDIRGPVLEEAMRMRANGEQILRLNTGNPA
                     EFGFTAPDEVIRDLIHNARKSEGYSNSKGIFSARKAIMQYCQLKKFPNVDIEDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGKAVHYVCDEVAEWYPDLAD
                     MESKVTSRTKAIVLINPNNPTGALYPKEILKGIIDIARRHELIIFSDEIYDRMVFDGA
                     VHIPIATLAPDLFVVTMNGLSKSHRICGFRVGWMVLSGPKKHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGYQSVDELLMPGGRLYEQREFITKAINDIPGLSAVKPKAGLYV
                     FPKIDREMYRVEDDEQFVLDFLKQEKVLLVHGRGFNWKDPDHFRIVYLPRVDELAEIQ
                     EKMSRFLRQYRR"
     gene            7010..7798
                     /gene="codY"
                     /locus_tag="AC1PIW_001083"
     CDS             7010..7798
                     /gene="codY"
                     /locus_tag="AC1PIW_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HIP6606026.1"
                     /translation="MTTLLEKTRNITSILKRSEEQLAEELPYNAIAEHLSAIIDCNSC
                     IINSEGEVLGYHMSYETNNDRVEEFFQNKQFPEGYVKAVAQVYDTQVNLPVESELTAI
                     PVESRSTYPNGLTTIAPIHVTGIRFGSLIIWRNDEQFHDDDLILVEIAATVVGIQLLN
                     FQREEDEKNIRRRAAVNMAVNTLSYSEMKAVAAILGELDGNEGQLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLIPAIFDEIKKRDY"
     gene            7811..8341
                     /locus_tag="AC1PIW_001084"
     CDS             7811..8341
                     /locus_tag="AC1PIW_001084"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HIP6606027.1"
                     /translation="MDYIVDFVADEGKLTAGKSAQAISKRIAQVTQEAFENGDYIFFA
                     IDGHEEGDEFHPEAQLFPSHNIIGTQGRDLYGPLADFYQKHKGHARVRWMDKRHYSTF
                     SGTDLDVRLRERGVDTVVLTGVLSDICVLHTAIDAYNKGYRIEVVSSAIAALTEENHQ
                     FALNHLRHVLGATIID"
     gene            8595..8942
                     /gene="rplS"
                     /locus_tag="AC1PIW_001085"
     CDS             8595..8942
                     /gene="rplS"
                     /locus_tag="AC1PIW_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIP6606028.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVISRKGQGISEMYTVRKISGGVGVERTFPIHTPRVDKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            9103..9777
                     /locus_tag="AC1PIW_001086"
     CDS             9103..9777
                     /locus_tag="AC1PIW_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HIP6606029.1"
                     /translation="MSEQFIPSVLSDLRQDIVQMPEVIKECSGIRIYGRRIRSVLFTT
                     DVSIIANHNADAILAVYPFTPSPAIIKSIMLVASVPVLAGVGGGLTTGMRSANMSLLS
                     ESEGAYAVVVNGPTDVKTIEAINKVVDIPIIYTVVSEKSDLTSRIKAGVDILNVSCGM
                     ETPRVVKKIREAFPDFPIIATGGPTEDSIRRVIEAGANAVSYTAPSNGELFKGKMEKY
                     RKHAKD"
     gene            9994..10296
                     /gene="gatC"
                     /locus_tag="AC1PIW_001087"
     CDS             9994..10296
                     /gene="gatC"
                     /locus_tag="AC1PIW_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIP6606030.1"
                     /translation="MKISEAEVRHVAKLSKLEFSDQETAEFATSLSKIVDMVELLNEV
                     DTTGVAVTTTMADRKNVLRADIAQKGESREELFKNVPESQDNFIKVPAILDGGGDA"
     gene            10296..11762
                     /gene="gatA"
                     /locus_tag="AC1PIW_001088"
     CDS             10296..11762
                     /gene="gatA"
                     /locus_tag="AC1PIW_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HIP6606031.1"
                     /translation="MTFNNKTIDELHDLLVKKEISAVELTKATLEDIKSREGAVDAFL
                     TITEDAALAQAAALDEKGIDADNVMAGIPLAVKDNISTKGILTTAASKMLYNYEPIFD
                     ATSVAQAYAKDMIIVGKTNMDEFAMGGSNENSAFKPTKNAWDQTKVPGGSSGGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGMKPTYGTVSRFGLIAFGSSLDQIGPFSQTV
                     KENAQLLNVISGHDVKDATSTINEIADFTSKIGQDIKGMKIALPKEYMGEGIDPQVKE
                     TILKAAKHLESLGAIIEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGFRAEDA
                     TNLDEIYVKTRSQGFGEEVKRRIMLGTFSLSSGYYDAYFKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPTVAFGLDTLNHDPVAMYLADLLTIPVNLAGLPGLSIPAGFVEGLPVG
                     LQLIGPKYSEETIYQVAAAFEATTDYHKQQPVIFGGAN"
     gene            11762..>13198
                     /gene="gatB"
                     /locus_tag="AC1PIW_001089"
     CDS             11762..>13198
                     /gene="gatB"
                     /locus_tag="AC1PIW_001089"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIP6606032.1"
                     /translation="MNFETIIGLEVHVELNTNSKIFSPSSAHFGEDPNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKDMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEIELEDGSTKKIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEIIQYTGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FNYVRKGLQHEVERQAKILRSGGQIQQETRRYDESTGETILMRVKEGSADYRYFPEPD
                     LPLYEIDDSWIEEVRAELPVFPKARRAHYVENLGLTAYDAGQLTSTKALSDFFEAAVA
                     AGGDAKQVSNWLQGEVAQFLNAEGKTIEQIALTPENLVEMIALIADGTISSKIAKKVF
                     VHLAKEGGSAKAYVEKAGLVQISDPAVLIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVAQQLLAQELAKLLD"
BASE COUNT         3675 a         2270 c         3222 g         4031 t
ORIGIN      
        1 attccgccat agctcagttg gtagagcgca tgactgttaa tcatgatgtc acaggttcga
       61 gccctgttgg cggagtaaag agaaatcttt agtatatggt ccgttggtca aggggttaag
      121 acaccgcctt ttcacggcgg taacacgggt tcgaatcccg tacggactat atttggagac
      181 tgcttaagtc tctttttttt gttttttgag gtgtatgggc tttaatatgg ctcctttttc
      241 ttttcttgtt actttgtcta ttcattgttt gttgatataa tagagatatg aaaagactat
      301 gtgatgaatt atcggttttg ccagggattg gtccaaaatc ggctgaaaaa ttcttaaaaa
      361 ttagcattca gaatattaat gatttgctga cttactatcc atttagatat gaagattttg
      421 agagtaagtc tatttatgat ttgcaggatg gggagaaggc agtggtagtt ggggaggtgg
      481 tatctccagc taatgtacag tattatggtt acaaaagaaa tagactccgt ttttcaatga
      541 aacagggtga ggttgtctta gcagtttcgt tttttaatca gccttattta gcagataaga
      601 ttgttttggg gcaggatatt gcagtttggg gaaagtggga taaggctaag gcaagtttga
      661 caggaatgaa aattttggct caagtatcag atgagctcca acctgtctat catgtggcgc
      721 aagggatttc gcaagtaaat ttggttaagg ctattaaaac agctattgat cagggatatt
      781 tgcatttatt agaggagaac ttgccatcag ttttacgaga gcgctatcgg ttaatgaatc
      841 gccgagaggc ggtttttgcg atgcattttc ctacaaattt agaagagtat agacaagctt
      901 tgcgacgtat gaagtttgag gaattatttt attttcaatt acagttgcaa atgctgaaag
      961 ccaacaatcg tgatattttg aatggtttga agattgccta tgatgctgat agattggcta
     1021 tgcaaataag acaattgccg tttgtcttaa cagatgctca gtctggggct ttggctgaaa
     1081 tactatcaga tatgaagtcc tatgggcaca tgaatcgttt gttacaaggg gatgttggtt
     1141 cagggaagac agtagttgct ggattggcta tgtttgctgc tgtgactgcg gggatgcagg
     1201 ctgcaattat ggtgccgaca gaaattttgg ctgagcaaca ctttgagagt ttgcgccagc
     1261 tttttccaga actttctatt gctcttttga caggtggaat gaaagctgcg gagcgtcgta
     1321 ctgctttaga ggcgatttct agtggtcagg tagacatcat tgtaggaact catgctctta
     1381 tacaggaaag tgtgacgtac cataagcttg gtttggttgt gacggatgag caacatcgtt
     1441 ttggtgttaa acagcgccgt ctgtttcgtg agaagggtga taatccggat gtgcttatga
     1501 tgactgctac acctattcct cggacgttag ccattacagc ttttggagat atggatgtat
     1561 caattattaa tcagttacct gctggtcgaa agccaattat cactcgctgg gtcaaacatc
     1621 aacagctgcc cacggtctta gattggttgg agcgggagtt agaagtgggt gctcaggttt
     1681 attttatctc tccgctgatt gaagaatctg aggctctaga tttaaagaat gctgtagatc
     1741 tacaatctga tttacaggcg cattttggag agcaagttac agtggatttg ttacacggta
     1801 agatgaagaa tgacgagaaa gatgccatca tgcaggcttt taaggagcga aaaacgaata
     1861 ttttggtttc taccactgtt attgaagtcg gagtcaatgt gcccaatgca acggtgatgg
     1921 ttattatgga tgctgatcgt tttggtttaa gccaattaca tcagttgaga gggcgtgtag
     1981 gacgtggtca taagcagtct tatgcagtgt tagttgctaa tcctaaaaca gagtctggta
     2041 aggaacgcat gaaaattatg acagagacga cagatggttt tattttggct gaagctgact
     2101 taaaaatgcg tgggtctgga gaaatatttg gaactcgtca atcaggtttg ccagaatttc
     2161 aagttgcaaa tattatcgag gactatccaa ttttagagga agcaaggcga gttgctagtc
     2221 agattgttag cgtggaaaat tggcaggagg accctaattg gtcgattttg cttgctaatt
     2281 tgaaagatcg agaggaattg gactagtcat ataaaaaagg ccaacacaca tgtgttggtc
     2341 ttttgacata ttgtatagtt cgtcagcgat taaccttgga tgtaatctgc caagctttgc
     2401 tcttccaagc ctgcattgag agcgataagg agtttgagtc gggctttttg agcattcaat
     2461 tctttaacaa atagaatgcc gtctttctcc agttggatgc cacctccttg gtaggcatag
     2521 acaggctcag caattccgtt aaaacagcga gataccagaa taatgggtat cttttgatca
     2581 aggagtttct taatggctgg aaggatggta ggcggaagat ttccggcgcc gagtgcttca
     2641 ataactaaac cggaaattga gcttgcagtg agtgaatcaa ggagaatgga gtccatatct
     2701 gcataagctt ttatgattgg gaccgttcct gtgactgttg ataagtcaaa tcgaacacgt
     2761 ggttcagcag ttttaaaata gagaatctct cgtttggtga ctaatcccag tggtccatgg
     2821 gttggtgttt gaaaggttga gacattggtg gtatacgttt ttgtaacata tttagcagca
     2881 tgaatttcat cgttcatgac cactagaaca cctttatctg cggatttatc atcagtagcc
     2941 acacgtaggg ctgttcggta gttataaatc ccgtcgctac ccagttcatt tgaggaacgc
     3001 atggctccgg tcaatacaat tggtttttct ggaatagcca ttgtatcaag gaaataggca
     3061 gtctcttcta aagtatctgt gccatgtgta atgacaaaac catcgaagtt catttgttct
     3121 tctttgattt tttggtatag agtcatcatg tgttctaagc gaatgtgtgg acttggaaca
     3181 ttgagaaaat ccaaggatgt cacttgagtt tcttctagtg ggctatcaat ttgggtcatt
     3241 ggattggttt tattcgctgc aacttcaccc tgatgattgg tttgcataga aatggttcca
     3301 cctgtatgca gtactagaat ttttttcata aattttcagt ttctttcaaa gtgtgatata
     3361 ctaattgtaa cataattctt agaaagtagg tgagttggtt gacgataaag gctgtatttt
     3421 tcgatattga tggtacctta ttgaccgaca atcgtatggt tagtagttca actattctcg
     3481 ccattaatgc tttgaaagag aaggggattt tagttggtct agctacagga cgtgatcctc
     3541 gttttgtttt gcagtatatg gctagtttag ggctagattt ggccattgct tataatggtc
     3601 aatatatttt ttcaagagaa gaagttattt atagtcagag tttagagcca aagcaaattg
     3661 aacaaattat ggagtatgcg caaactcacc ataaggattt atcttttgga actgctaaag
     3721 gcatatttgg tagtaagatt atgagtgctg gaacaggaaa ttttgcatat cgtgtgacgc
     3781 gtatgattcc ggagtcctgg gctggaatca taaattttat tttcaatcgt ttggtacgtt
     3841 ggattagtcc tcaacaggaa acgaatttga aaggttttct ctttcaacca atctatcagc
     3901 tgatgttgct gacgacggaa agggagacac aatcattgga ggtgctcttt ccgaacttgt
     3961 cttttacgcg ttcgagtccc tatgcgactg atattatcag taagggaaat tcaaaattat
     4021 cgggtattgt aaaggttgca gatcgatatg gttttgagtt agatgaagtt atggtattcg
     4081 gggattctaa taatgatttt gaaatgttga atgaaattca gcactcggta gcgatgggaa
     4141 atgggacgaa aaaggttaaa cacgcagctt cctttgttac ggatacaaat aatcgtgatg
     4201 ggatttataa ggcattaatt cattttggtg tgattgaggg ataagatgtt tcagagcaag
     4261 gacgataatt ttaatagggt aaaggatttt cattttttaa tggatggtga aacacaagaa
     4321 cttcccagtg tgtatgatgg acagacggct ttgtatcgcg ctggttttaa attggaggag
     4381 ttggtagaat ttctacatgc agcttcagaa tctgaagttg agtttcatga ttttattcaa
     4441 caacttcatc aggatttaga tactgctgct gctaaggtat ctggcaagag aggtttcggg
     4501 gtttcgatgc aggatcaggt agatgctttg ctggatattc tttattttac atatggttcg
     4561 tttgttttga tgggggttga tccagaacca atttttcaga tagtccatac tgcaaatatg
     4621 gggaaaacat ttccagatgg gaaggcccat tttgatccaa ttacccataa aattttaaaa
     4681 cctgatgatt gggaagaacg gtttgctccg gaggaaaaga ttcaggaaga gctgaaaaga
     4741 cagatgaaaa gacttgattc gtaaatcaag tcttttggtt tataaacttt tttctccatc
     4801 gcgaacaatg agtaaatcga tagttgcgtg gcgcatgata tattcggatg aggatccgat
     4861 taggagacgt tcaaaggcgt ttagaccagt tgctccaagt aacataagat ccgcaccggt
     4921 ttcctttgga atatcgatag ccagtagggt tttagggtta ccgaattcaa tgcgaatctg
     4981 tacatcggtc agaccggcgt tgagagcttc ttgtttgtat tcttcgagga ggagctccgc
     5041 ctctctttct aaagattcat agacagaggc gtcaaatgca acaacattgt ggagagcacg
     5101 ggtgtcaatc acatgggcga taatcagacg tgcttggttg cgtagtgcaa catgaatggc
     5161 tttatgaaga gctaattctg ctcctttaga gccgtctaca gcgacgagta ttgttttgta
     5221 agattgtgtc ataatagtca ctcctttcag aagagctttg tcccgaaata ggtagagtag
     5281 ttccttacat ttttattata agccttttga gagaaaatgt aaaggattct tgtacttttt
     5341 tagcttttac agtacaatag aaaagataga aagttgaggt gacgacatga agcaatttaa
     5401 caagtcaaca aaattggatg atgtagcata tgatattcgt gggcctgttt tggaagaggc
     5461 catgcgcatg cgtgccaatg gagagcagat tttacgtttg aatacgggga atcctgcaga
     5521 atttggtttt actgctccag atgaagttat tcgtgacttg attcataacg cccgtaaatc
     5581 agagggatat tcgaacagta agggaatttt ttctgcacgt aaggctatca tgcagtattg
     5641 tcagctaaag aaatttccaa atgttgacat tgaagatatt tatcttggaa acggcgtgag
     5701 tgagctgatt gttatgtcta tgcaaggttt gctagataat ggtgatgagg tgttggtgcc
     5761 aatgccagac tatcctctct ggactgctgc agttagtttg gctggtggta aagctgttca
     5821 ctatgtttgt gatgaagtag ctgaatggta tccagatttg gcagatatgg agtccaaggt
     5881 gacttctcga accaaggcaa ttgtccttat caatccaaat aatccaactg gtgctctcta
     5941 tccgaaagaa attttaaaag gaatcattga tattgctcgt agacatgaat tgatcatttt
     6001 ctctgatgag atttatgatc gtatggtctt tgatggagct gttcatatcc ctattgcgac
     6061 actggctcca gatttatttg ttgtgacgat gaatggactg tctaaatcac atcgtatttg
     6121 tggtttccga gtaggttgga tggtcttatc tggtccgaaa aagcatgtaa aaggttacat
     6181 tgaaggcttg aatatgttgt ccaatatgcg tttgtgttca aacgttttgg ctcaacaggt
     6241 tgttcaaact tctttgggag gttaccaatc ggttgatgag ctcttgatgc ctggcggacg
     6301 cttatatgaa caaagggagt tcattaccaa ggcgattaat gatattccgg gactttctgc
     6361 tgtaaaacct aaggctggtc tgtatgtatt cccaaaaatt gatcgtgaga tgtacagagt
     6421 tgaggatgat gaacaatttg tattggattt cctcaagcaa gaaaaagtac ttttggtcca
     6481 tggtcgaggt tttaactgga aggatccaga tcatttccgt attgtgtatt taccacgtgt
     6541 tgatgagttg gcagaaattc aagaaaaaat gtcacgattc ttgcggcaat atcgtagata
     6601 atgggagtgg gaaagaactc aaccattcat aagaagagtt cgtcaacaag tctttatttc
     6661 tagttgttga gctgaaacag tctattccca gactgtttcg ctcccacctc cgcacagctc
     6721 aaactgtctg ggagacagtt tgaggttgga gatgaagcga actctgttcg catcagtcgt
     6781 ataggtcaga ttttgaatgt aaaacacgaa cgaatctgcc aatcaaccac tgcgctgaga
     6841 tgttgacact aactttgaga agcggtgctg ggctttttgc ccagcctatt tctttttgct
     6901 tggatgaaat tgtttgtgtt tttctaacaa cggtaaatat tctgataatt gttgaaattt
     6961 tatgtatttt ttgctatact gaaagcaatt acagagtaaa gaggaaaata tgacaacatt
     7021 attagagaag acacggaata ttacttctat tttgaagcgt tccgaagagc aattggcaga
     7081 agaattgcct tacaatgcta ttgctgaaca tctatcagct attattgatt gcaattcttg
     7141 tattattaat agcgaaggtg aagttttggg ataccacatg agttacgaga caaacaatga
     7201 tcgtgtggaa gaatttttcc aaaacaaaca attcccagaa ggatatgtaa aagcagttgc
     7261 gcaggtttac gatacgcagg ttaatttgcc tgtcgagagc gagttgactg ccatccctgt
     7321 cgaatcacgc tcgacttatc caaacgggct gacaacgata gcgcctatcc acgtaacggg
     7381 gattcgtttt ggttcgctta ttatttggcg gaatgatgag cagtttcacg atgatgattt
     7441 gattttggtt gagattgcgg caacagtagt tggtattcag ttacttaatt tccaacggga
     7501 agaagacgag aagaatatcc gtcgtcgtgc ggcagttaat atggcggtaa atacgctatc
     7561 ttactcagaa atgaaggcag ttgcagctat tttgggtgaa ttggatggca atgaggggca
     7621 attgactgct tctgtgattg cagatcgtat cggtattaca cgatcggtga ttgtgaatgc
     7681 actgcgtaag ttggagagtg cagggattat tgaaagtcgt tctttgggaa tgaaggggac
     7741 ttatttgaaa gttctcatcc cagctatttt tgatgaaatt aagaaacgtg actactaaaa
     7801 tgacaaaagc attgattata tagtagattt tgtggcagat gaaggaaagc tgactgctgg
     7861 aaaatcagct caggctattt ctaaacgaat tgctcaggtt acgcaagaag cttttgaaaa
     7921 tggagactat attttctttg cgattgatgg tcatgaggag ggggatgaat ttcatcctga
     7981 agctcagctt ttcccaagtc ataatatcat tgggacgcaa gggcgtgact tgtatggtcc
     8041 tttagcagat ttttatcaaa aacataaagg gcatgcgcgt gttcgttgga tggataaacg
     8101 tcattattct actttttctg ggacggattt ggatgttcga ctaagagaac gtggtgtaga
     8161 tacggttgtc ttgacgggtg ttttgtctga tatttgtgtt cttcatacag ctattgatgc
     8221 ttataataag gggtatcgta ttgaggtggt ctcatcagcc attgctgcat tgacggagga
     8281 gaatcatcag tttgctctta accatttgcg tcatgtactc ggtgcgacaa ttattgatta
     8341 atagtatagt gaccagtctg tggactggtt tttacaggaa tctgtatcat taacactctt
     8401 cgaaaatcaa actcagacct tgttgactta acttgtaaaa gaagcggtgt gattgaccat
     8461 gataaattta tcaaaatgct ttacataggt gattacttgg tgtataatat agtctgtgtg
     8521 taatggacgc acaaaaatac agtttatccg ctgggtttaa aagcacctaa gattgacaaa
     8581 gataggagaa taaaatgaat ccattgatcc aaagtttgac agaaggtcaa cttcgtactg
     8641 acatcccttc attccgtcct ggtgacactg tgcgtgttca cgctaaggtt gtcgaaggta
     8701 accgcgaacg tatccagatt ttcgagggtg ttgttatctc tcgtaaaggt caaggcatct
     8761 cagaaatgta cactgtacgt aaaatctctg gtggtgtagg tgttgaacgt acattcccaa
     8821 tccacactcc acgtgttgat aagattgaag tagtacgtta cggtaaagta cgtcgtgcta
     8881 aattgtacta cctacgtgca ttgcaaggta aagcagcacg tatcaaagaa atccgtcgtt
     8941 aagatagtcc tacagactat tttggtcgga gaatgggagg cagcgaaagc tgtctctttt
     9001 tttaaaaaaa atctgttgaa aaagtaccaa attgtgaaag cataaaatgg aaataatatg
     9061 ttatactata tattattgta gcgagtatag aggaggttgc ggatgagcga acagtttatt
     9121 ccatctgttt tatcagattt acggcaagat attgttcaga tgccagaagt tatcaaggaa
     9181 tgtagtggaa ttcgtattta tggtcgtcgc attcgttcag tcttatttac aacggatgtg
     9241 tctattattg ctaatcacaa tgcagatgct attttggctg tttatccatt tacgcccagt
     9301 ccagctatta tcaaaagtat tatgttggtt gcgtcagttc cagtcttggc tggtgttggt
     9361 ggaggcttga cgacaggtat gcgttcagct aatatgagtc tcttgtctga gtcggaggga
     9421 gcatacgctg ttgtggtaaa tggaccaacg gatgtaaaaa caattgaggc aatcaataaa
     9481 gtagtagata ttcctatcat ttataccgtt gtttctgaaa agtctgattt aacctcacgg
     9541 attaaagctg gagtagatat tttaaatgtt tcttgcggta tggaaacgcc aagggttgtg
     9601 aagaagattc gagaagcttt ccctgacttt ccaattattg cgactggtgg tccgactgag
     9661 gactctattc gtcgggttat cgaagctgga gccaatgctg tctcctatac agcaccaagt
     9721 aatggggagc tttttaaagg gaaaatggaa aaataccgca aacatgcaaa ggattagctc
     9781 ataaaaagat aggctaagca aacagtaagt tttattgcta gatgaaactt gctgtttttt
     9841 atcgttttac ggtgtgagat taggaatact tcgcaattta aagaatggat ttttaggagg
     9901 aaataggtaa catattaggt catttctacc tttgtctgag gctggaaata tggtataatg
     9961 agaagataga tttccattag gaggaaagta agaatgaaga tttctgaagc tgaagtccgt
    10021 cacgttgcca agctgtctaa gctggaattt tcggaccagg aaacagcgga atttgcgaca
    10081 agtttgagca agattgtcga tatggttgaa ttgctcaatg aagtagatac gacaggtgtt
    10141 gcggtaacga ccactatggc tgaccgtaag aatgtcttgc gagcagatat tgcccaaaaa
    10201 ggtgagagcc gtgaggagct ctttaaaaat gtgcctgaat cacaagataa ctttatcaag
    10261 gtaccagcta ttctagacgg gggaggagat gcctaatgac ttttaacaat aaaaccattg
    10321 atgaattgca tgacctcctt gtcaaaaagg agatttctgc ggttgagttg accaaggcaa
    10381 ctttggaaga catcaaaagc cgtgagggag cagtggatgc tttcttgact atcacagaag
    10441 atgcggcctt ggcgcaggct gctgcccttg atgaaaaagg gattgatgcg gacaatgtta
    10501 tggcagggat tcctttggca gtcaaggaca atatctctac taaggggatt ttgaccacgg
    10561 cagcttcaaa aatgctttat aactacgagc cgattttcga tgcgacatca gttgctcagg
    10621 cctacgcaaa ggatatgatt attgtcggta agacaaacat ggatgagttt gccatgggtg
    10681 gttctaatga gaactctgcc ttcaaaccga ctaaaaatgc ttgggaccag acaaaagttc
    10741 ctggtggttc ttcaggtggc tcagctgctg ctgttgcatc tggtcaggtc cgtttgtcac
    10801 tcggttctga cacaggtggt tccattcgtc aaccagcagc ctttaatggt atcgtcggta
    10861 tgaaaccgac ctacggaacg gtgtcacgtt ttggtctgat tgcctttggt tcatctcttg
    10921 accagattgg tccattctca cagacagtta aggaaaatgc ccagttgctc aatgtcatct
    10981 ctggtcatga tgtcaaggat gcaacatcaa cgatcaatga aattgcagac ttcactagca
    11041 agattggtca ggacatcaag ggcatgaaaa tcgctcttcc aaaagaatac atgggcgaag
    11101 ggattgatcc gcaggtcaag gaaactattc tcaaggcggc taagcacttg gaaagcttgg
    11161 gagcaatcat tgaagaagtt agtctgccac attctaagta tggggttgct gtttactata
    11221 ttatcgcatc atcagaggcg tcttctaact tgcagcgttt tgacggtatc cgctatggtt
    11281 tccgtgcaga agatgcgacc aacttggatg agatttatgt gaaaacccgt agccaaggtt
    11341 ttggtgagga agtcaaacgt cgtattatgt taggtacatt tagcttgtca tctggttact
    11401 acgatgccta cttcaagaag gctggtcagg tgcggacctt gattatccaa gattttgaaa
    11461 aggtctttgc ggactatgac ttgattttgg ggccaactgc tccgacggtt gcctttggtt
    11521 tggacacgct caaccatgac cctgtggcca tgtacttggc ggacttgtta acaattcctg
    11581 taaacttggc aggtcttcct ggtctttcta ttcctgctgg ttttgtagaa ggcttgccag
    11641 ttggtttgca gttgattggt ccaaaatact cagaagagac catttaccaa gtagctgctg
    11701 cctttgaagc gacgacagac taccacaagc aacaaccagt gatttttgga ggtgctaact
    11761 aatgaacttt gaaacgatta ttggtctaga agtccatgtg gagttgaata ccaactcgaa
    11821 aattttctca ccttcatctg cgcattttgg tgaggatcca aatgccaata ccaacgtgat
    11881 tgactggtct ttccctggcg tccttcctgt tcttaataag ggtgttgtgg atgctggtat
    11941 taaggctgcc ttggctttga acatggacat tcacaaggac atgcactttg accgcaagaa
    12001 ctatttctat cctgataacc ctaaagccta tcagatttct cagtttgacg agccgattgg
    12061 ctacaatggc tggattgaga ttgagctaga agatggttca accaagaaaa tccgtatcga
    12121 gcgtgcgcac ttggaagagg atgcaggtaa gaatacccat gggacagacg gctattctta
    12181 tgtggacctc aaccgtcagg gcgtgccatt gattgagatt gtatcagaag cggatatgcg
    12241 ttcgcctgag gaggcctatg cctacttgac agcccttaaa gaaatcattc agtacactgg
    12301 tatttcagat gtgaagatgg aagagggttc tatgcgcgtg gatgccaaca tctctcttcg
    12361 tccttatggt caggagaaat tcggtaccaa gactgagttg aaaaacctca actccttcaa
    12421 ctatgtgcgc aagggcttgc agcatgaagt agaacgtcag gcgaaaatct tgcgttcagg
    12481 tggtcaaatc cagcaggaaa ctcgtcgtta cgatgaatct actggtgaaa ccattctcat
    12541 gcgtgtcaag gagggttcag ctgactaccg ttacttccct gagccagacc tgccactcta
    12601 tgagattgat gatagctgga ttgaggaagt tcgtgcagaa ttgccagtct ttccaaaggc
    12661 tcgccgtgct cactatgtag aaaacttggg cttgaccgcc tatgatgcag gtcaattgac
    12721 gtctaccaag gccttgtctg acttctttga agcagcggtg gcagcaggtg gcgatgccaa
    12781 acaagtttct aactggttgc agggtgaagt ggctcagttc ctcaatgctg aaggtaagac
    12841 tattgagcaa atcgccttaa caccagaaaa cttggttgag atgattgcct tgattgcgga
    12901 tggaaccatt tcatctaaaa tcgccaagaa agtctttgtt cacttggcca aagaaggtgg
    12961 ctctgctaag gcttatgttg agaaggctgg tttggtgcag atttcagacc cagcagtcct
    13021 cattccgatt atccaccaag tctttgcgga taacgaagcg gctgtagctg acttcaagtc
    13081 tggcaaacgc aatgcagaca aggcttttac aggtttcttg atgaaagcaa ccaagggaca
    13141 agccaatcca caagttgcac aacaactctt ggctcaggaa ttggctaagt tgttggat
//
LOCUS       DBNFCK010000048        13119 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.048, whole genome shotgun sequence.
ACCESSION   DBNFCK010000048 DBNFCK010000000
VERSION     DBNFCK010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13119
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.048"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..789
                     /locus_tag="AC1PIW_001090"
     CDS             <1..789
                     /locus_tag="AC1PIW_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIP6606033.1"
                     /translation="IVRAKNRLHKVLQVTFPELENILSTPTGEQYWNLVITFPCKDFV
                     LDLSKNELSKSIRQSTSKRISDKRVAYLAEKLTALANQSYCAVKKTSPILEEVHYYAK
                     ELLRLSEQRQAVLDQMVELAQPLPEYDILLSIPGIAETTATSIIGELGDIRRFQSANQ
                     INAFIGIDLRHYESGNFLAKEHITKRGNPYARKILFKCIHNIASVSHTNPCHIADFYE
                     KRKRQSQTTSTKPHTIASIHRLIRTMYYLIMHNKLYDYASTQNR"
     gene            complement(1226..1876)
                     /locus_tag="AC1PIW_001091"
     CDS             complement(1226..1876)
                     /locus_tag="AC1PIW_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIP6606034.1"
                     /translation="MYQTILFDLDGTLTDSGQGILNSVAYALEKMGIEEPDTANLNRF
                     IGPPLYESFSRFYQLSPEDTQSAVDAFRVYFKEKGMFENQLYPGIIPLLEELRTAGKT
                     LVIATSKPEIFAKQILEHFGISHYFDVIAGASLDSSRISKADVICYAINQLEAFPNHA
                     VMIGDREHDIEGARMHQLPAIGVLYGYGNKQEFEKAGATMIVETVQDLKRVLLTTE"
     gene            1984..3111
                     /locus_tag="AC1PIW_001092"
     CDS             1984..3111
                     /locus_tag="AC1PIW_001092"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HIP6606035.1"
                     /translation="MHILIAPDSFKESLSATKVAEAIKKGFSKVYPQAGYDLIPLGDG
                     GEGTVESLMQALSLDGTQTWVTGPFGDKIKVSYAVKDDLAVFEMAEIVGLASIPHERR
                     SPLFIKTQGVGELIVELVQNGVKRIFIGVGGSASHDGGIGMAAGLGVKFYNREGKEVE
                     AIGAHIGEIVSFSTEDMLVDLSQVRIDLVTDVENPLCGPAGATFVFAGQKGLTSAEFE
                     LVDKKMESFYKLVNPMLLDLAGAGAGGGMAAGLVQFAGARIRKGIDFVLDQLDFDHRV
                     TKAGLVVVGEGRMDAQSLSGKTPIGVARRTPTGIPIIAICGSLKDDLPEFPFENICAA
                     FPIIASVESLEDTLQKAEKNLVRTAEQVARILQLGGQQRWN"
     gene            3102..3272
                     /locus_tag="AC1PIW_001093"
     CDS             3102..3272
                     /locus_tag="AC1PIW_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606036.1"
                     /translation="MELVKYVAIILFIFICLAKLVSVMRLSEKERVTEMNVIKANGAM
                     FFFNEKMKEADC"
     gene            complement(3338..3790)
                     /locus_tag="AC1PIW_001094"
     CDS             complement(3338..3790)
                     /locus_tag="AC1PIW_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1694 domain-containing protein"
                     /protein_id="HIP6606037.1"
                     /translation="MNDLNTTILQKASGETRLNPDEQRLYMGTYRERVVLIVSFDDLS
                     SEVVGNKFDTICQKLANRYSPLFLKLSPALSDKQQISLLKIAQTFGITTAIIDEKIGQ
                     SPYALVFHTNHAVDNEEVSLESIFPNLISKPEEKKEDAKPSFWKKLFG"
     gene            3940..5247
                     /gene="eno"
                     /locus_tag="AC1PIW_001095"
     CDS             3940..5247
                     /gene="eno"
                     /locus_tag="AC1PIW_001095"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-displayed alpha-enolase"
                     /protein_id="HIP6606038.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYLGLGTQKAVDNVNNVIADAIIGFDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYTYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPSFKEGLRWGAEVFHALKKILKARGLVTAVGDEGGFAPKFEGTEDG
                     VETIIEAIEAAGYEAGENGIMIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQI
                     DYLEELVNKYPIITIEDGMDENDWDGWKALTERLGKRVQLVGDDFFVTNTDYLARGIK
                     EGAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAG
                     QIKTGSLSRTDRIAKYNQLLRIEDQLGEVAVYKGLNSFYNLKK"
     gene            5557..5778
                     /gene="relB"
                     /locus_tag="AC1PIW_001096"
     CDS             5557..5778
                     /gene="relB"
                     /locus_tag="AC1PIW_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HIP6606039.1"
                     /translation="MSTVTIRLNQEEEVFFKSYAQLTGQSLSSLFKKALERDIEDEYD
                     LKIYHQAYDEYKADPETISHADFKKELGL"
     gene            5780..6040
                     /locus_tag="AC1PIW_001097"
     CDS             5780..6040
                     /locus_tag="AC1PIW_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE family
                     toxin"
                     /protein_id="HIP6606040.1"
                     /translation="MYHLEYSKKAQKQIMKLDRQIQRLLFAWIDKHLEGTDNPRANGK
                     GLTGNHANEWRYRIGDYRLICDIQDDKLVILALEFGHRKDVY"
     gene            6207..6848
                     /locus_tag="AC1PIW_001098"
     CDS             6207..6848
                     /locus_tag="AC1PIW_001098"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIP6606041.1"
                     /translation="MIEWIFFDLGSTLLDEEAAYGYYIDKCVKKLESLDIEVSSDSYK
                     KKMVEYAHKSLDPIRSTWHYFAPTEPRPLWTNEGVSLYPETIDALEKLSQNYRLGIIA
                     NQSSSIRELLKEWGIESYFQLIILSEEVGLSKPNTAIFTLALQKTNIPADRVVYVGDR
                     FDNDILPAKSLGMWTVRILTGFGKHASENEKLKSDWIIPSLQEITNIFEQTKN"
     gene            complement(6881..7066)
                     /locus_tag="AC1PIW_001099"
     CDS             complement(6881..7066)
                     /locus_tag="AC1PIW_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606042.1"
                     /translation="MNPVDLFNQVKELIANKDFDGAKQFIEENKDQFGEYLEQAKGLL
                     SGSEGVNGLLDKVKGLF"
     gene            complement(7130..8134)
                     /locus_tag="AC1PIW_001100"
     CDS             complement(7130..8134)
                     /locus_tag="AC1PIW_001100"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HIP6606043.1"
                     /translation="MAIYFGTYTKRESKGIYKANFDSETGQLSNLELVTEEPNPTYLA
                     FDKAGHLYSVGAENGLGGIAAFNTDYTLLNHVVSEGAPLCYVAVDEARDLVYGSNFHK
                     GQLLVYKRLADGSLELADVAQHEGSGPHENQASAHVHFSDLTPDKFLVTCDLGCDQVV
                     TYKVSDQGKVEKIATYQAAPGSGPRHIVFHTVAKIAYLICELNSTIEVLIYDGWGQFE
                     HLQTVSTLPEDHTGFNGTAAIRITKDGKFIYGSNRGNDSIAVYKTLADASLELVEIVP
                     TNGKTPRDFTLSPDEKHLIVVHQDSDNATVFKRDAENGCLTELSHDFYVPEAVCVTFL
                     "
     gene            8270..9436
                     /locus_tag="AC1PIW_001101"
     CDS             8270..9436
                     /locus_tag="AC1PIW_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIP6606044.1"
                     /translation="MEDKHQKFSLTLFFRLFLNSQAVTFLLVTLLTFLTIFIFSKISF
                     LFRPIGSFLEIVLLPMILTGLLYYLLNPMVDWMEKHKISRTVGISILFVLISLLIIWG
                     LAVAIPSIQEQVTSFAQNLPSNIQKIEGQVTGLLQDQRFEQFRPTALEMLNKVNDQIV
                     AYAQKFSSSAVNWASNLISTASQIIVAILIMPFILFYLLRDGQYLNKHITQYLPTKWR
                     EPIGTVLSDVNGQLSNYVRGQVTVAIIVALMFSVMFSIIGLSYPITLGVMAGFLNLIP
                     YLGSFLAMIPAVILGLIAGPIMLIKVLVVFMIEQTIEGRFVTPLIIGSSLSIHPITIL
                     FVLLTAGQMYGVLGVLLGIPIYASIKVLVKAAFEWYKAHSGLYEDEENEVVNEQ"
     gene            9426..10655
                     /locus_tag="AC1PIW_001102"
     CDS             9426..10655
                     /locus_tag="AC1PIW_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIP6606045.1"
                     /translation="MNNSEKMLVCLDQQDLDKADKYFKRALVQDDSETLLSLAAYLEG
                     IGFFPQARQIYEQVKEEFPEVLINLAQIAFEDGLVEESFGYLEEISEDSSVYVEALLV
                     KADLYQAEGLSDVAREKLLEASHLSDEPIILFGLAELDMELELFNEAISYYAQLDNRE
                     IYELTGVSTYQRIGIAYASLGKFEAAIEFLEKAVELEYDDQTVFELAALLFEREEYQK
                     ANLYFKQLDTINPDFEGYEYAYAQSLHVEHKIDDALVMTQKGLAKNDFDANLLLQASQ
                     YAYELHDEAGAEDYLLRAKEVADDSNELALRLSNLYLEQERFEEVVALFDEEVDNVLA
                     RWNVAKAYQALERDDEAIELYDELAGEFAENPEFLADYVAVLRQLGRLDEAKEQASRY
                     IQLVPDDLAMQEFLNEE"
     gene            10659..11375
                     /gene="budA"
                     /locus_tag="AC1PIW_001103"
     CDS             10659..11375
                     /gene="budA"
                     /locus_tag="AC1PIW_001103"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019322637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HIP6606046.1"
                     /translation="MQVNRLFQYNTLGALMAGLYGGSLTVGELLEHGDLGLGTLDSID
                     GELIVLDGKAYQAKGAGEKPEVVEVPAEMKVPYAAVIFHEAEVIFKQRFEMTSDELHQ
                     RIESYYDGENLFRSIKIKGTFSRMHVRMIPKSASDVRFAEVASRQPEYTAENISGTIV
                     GIWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDYVISEGMVEVGPVDQLDQRFPVQDR
                     QYLFAKFNAKEVREDIDKAE"
     gene            complement(11401..13059)
                     /locus_tag="AC1PIW_001104"
     CDS             complement(11401..13059)
                     /locus_tag="AC1PIW_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990112 - RQC complex [Evidence IEA];
                     GO_function: GO:0001968 - fibronectin binding [Evidence
                     IEA];
                     GO_process: GO:0072344 - rescue of stalled ribosome
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein"
                     /protein_id="HIP6606047.1"
                     /translation="MSFDGFFLHHMTAELRANLEGGRIQKINQPFEQEIVLNIRSNRQ
                     SHKLLLSAHSVFGRVQLTQSDFTNPKVPNTFTMILRKYLQGAIIEEIRQLDNDRILEF
                     SVSNKDEIGDHIQATLIVEIMGKHSNIILVDKSEQKIIEAIKHVGFSQNSYRTILPGS
                     TYIRPPETHSLNPYTVSDEKLFEILSTQELSPKNLQQVFQGLGRDTASELANHLQIDR
                     LKNFRAFFDQATQPSLTDKSYAALPFANSPENQPHFESLSSLLDFYYQDKAERDRVAQ
                     QANELIKRVASELEKNRKKLIKQEQELADTETAELVRQKGELLTTYLHQVPNDQSSVR
                     LDNYYTGKELEIELDVALTPSQNAQRYFKKYQKLKEAVKHLTNLIEETKSTIVYLESV
                     DTMLGQASLAEIDEIREELIETGYLKRRHREKIHKRQKPERYLATDGKTIILVGKNNL
                     QNDELTFKMAKKGELWFHAKDIPGSHVVITDNLDPSDEVKTDAAELAAYFSKARHSNL
                     VQVDMIEAKKLHKPTGGKPGFVTYRGQKTLRVTPTEDKIKSMKI"
BASE COUNT         3703 a         2482 c         2965 g         3969 t
ORIGIN      
        1 attgttcggg ctaaaaaccg tctgcacaag gtcttacaag tcactttccc tgaattggaa
       61 aatatcttgt caacaccaac tggcgaacaa tactggaact tggtcataac ctttccttgc
      121 aaggacttcg tgcttgattt aagcaagaat gaactatcaa agagcattcg tcagtccacc
      181 tcaaaacgga tttctgacaa gcgtgtggcg tacttagctg agaagctgac agcactagct
      241 aatcaatcgt attgtgccgt caagaaaacc tctccaatac tggaagaggt gcattactat
      301 gcaaaagaat tgcttcggct ttctgaacag agacaagcag tcttagacca aatggtggaa
      361 ctagctcagc cattacctga atatgacatt ctgctctcta ttcctggaat agctgagacg
      421 actgcaacaa gtattattgg tgaactggga gatattcgcc gttttcagtc tgccaatcaa
      481 atcaatgcct ttatcggtat tgacctgaga cactatgaat ctggtaactt cctcgctaag
      541 gaacacatta ccaagcgtgg caatccctac gctagaaaga ttctgttcaa gtgtatccac
      601 aatatcgctt cagtcagtca taccaatcct tgccatatcg cagactttta tgagaaacga
      661 aaaagacaat cgcaaacgac ttcaacgaag ccgcacacga ttgcctccat acatcgtctc
      721 attcggacaa tgtattacct cattatgcat aacaaacttt acgattacgc ttcaacccaa
      781 aatcggtaaa actgtttatg ctctatcatt gtaacacctt atttcaaaaa atactagata
      841 aggtgtcatt ttgtcatgcc cttttatagc taaattagtg acaaaaataa ggtagcagcc
      901 tcaaaatagc tactgttatg ctttattttt ttgcttaaag ccttgataga cttgacaaat
      961 ggtagaaaaa gagttcgtca acaagtcttt atttctagtt gttgagctga aacagtctat
     1021 ccccagactg tttcactccc acccccgcac agctcaaact gtctgggaga cagtttgagg
     1081 ttggaaatga agcgaactct gttcgcatca gtcatagagg tcagatttgg agtgtaaaac
     1141 acgaacaaat ctgccaatca accactgcgc tgagatgttg acacgaaccc tgagaagcga
     1201 ggttggtctt tttgcccagc atcttctatt ctgttgtcag taaaaccctt ttcaaatctt
     1261 ggacggtttc gactatcatg gtggccccag ccttttcaaa ttcctgtttg ttcccatagc
     1321 cgtaaagaac accgatggcg ggaagttggt gcatgcgtgc tccttcaatg tcatgttccc
     1381 tatctccaat catcacagca tggtttggaa atgcttctaa ttgatttata gcatagcaga
     1441 tgacgtccgc cttactgatg cgactactat ccagactagc accggcaatg acatcaaagt
     1501 agtgtgagat accaaaatgt tctagaatct gtttggcaaa tatttctggc tttgaggtag
     1561 caattactag tgtttttcca gccgttctca attcttcaag cagagggatg attccaggat
     1621 agagttgatt ttcaaacatt cctttttctt taaaatagac acggaaagca tcaactgcac
     1681 tttgtgtatc ttcagggctg agttggtaga atcgagaaaa agattcatag agtggcggac
     1741 cgatgaatcg gtttaaattg gctgtatctg gttcttcgat tcccattttt tctagtgcgt
     1801 aggcgacgga gtttaagatt ccttgtccag aatcagtaag tgttccatct aagtcaaata
     1861 atatagtttg atacatactt atctcctttc tttcagtata acacagacga tgaaaaacag
     1921 aaaagattta ttgaaagctg actaggagcg tgatatacta ataatataaa ggaggttagt
     1981 accatgcata ttctcattgc tccggattca tttaaagagt ctctttcagc tacaaaggtt
     2041 gctgaagcga ttaaaaaagg attttctaaa gtttacccac aagcagggta tgacttaatt
     2101 cccctaggag atggtggcga agggacagtt gagagtctaa tgcaagcctt atccttggat
     2161 ggaacccaga cttgggtgac aggtccattt ggagataaaa ttaaggtttc ctatgcggtt
     2221 aaagatgatc ttgcggtttt tgaaatggct gagattgtag ggctggcaag tatcccgcat
     2281 gaaagaagga gtcctctctt tattaagaca caaggggttg gggagctgat tgtggagctc
     2341 gttcaaaatg gtgttaaacg catatttata ggagtaggtg gctctgccag tcatgatggt
     2401 ggtatcggaa tggcggcagg tttaggagtg aaattttata atcgagaggg aaaagaagta
     2461 gaggcaattg gagctcatat cggggagatt gtcagctttt ccacagagga tatgttggta
     2521 gatctatctc aggtcaggat tgatttggta acggatgtgg agaatccgct ttgcggacca
     2581 gcgggtgcca catttgtttt tgcaggtcag aaaggtctta cctctgctga atttgaacta
     2641 gtagacaaga aaatggagtc cttttataaa ttagttaatc caatgctact cgatttggca
     2701 ggagcgggtg ctggaggagg tatggcagca ggtttagtcc aatttgctgg tgctcgcatt
     2761 cgaaaaggca tcgactttgt cttggatcag ttggactttg atcatcgcgt caccaaagcg
     2821 ggtctggttg tggtcggaga agggcggatg gatgcccaaa gcttatcagg gaagactcca
     2881 ataggagtag ctagacggac gccgacgggg attcccatca ttgcaatttg tggtagcctc
     2941 aaagacgatc ttcccgagtt tccttttgaa aatatctgcg ccgcttttcc gataattgca
     3001 agcgttgaaa gtttggagga cacgcttcaa aaagcagaaa aaaacctggt ccgcacggca
     3061 gagcaggtgg caagaattct tcagttagga ggccaacaaa gatggaatta gtgaaatatg
     3121 tggctattat cttatttatt tttatttgtc ttgcaaagct agtatccgtt atgagacttt
     3181 ccgaaaaaga aagagtaacg gaaatgaatg taataaaggc aaacggagca atgttctttt
     3241 tcaatgaaaa aatgaaagag gcagattgct aggaatcctt tgtctaacct gtattatttt
     3301 tactttaata tttatactgc tttatcgtta aataagtcta accaaataat tttttccaga
     3361 aggaaggttt tgcatcttcc tttttttctt ctggttttga gataagattt ggaaatatgg
     3421 attctaagct aacttcttca ttatccacag cgtgatttgt gtgaaagaca agtgcgtagg
     3481 gagattgtcc tattttttca tcgatgatag cggtagtaat gccaaaggtc tgagcaattt
     3541 ttagtagtga aatttgttgc ttgtcagaca gggctggcga gagtttgagg aatagaggag
     3601 agtatctgtt tgctagtttt tggcaaattg tatcgaactt atttccgacg acttcactac
     3661 tcaaatcgtc aaaggaaacg atcaggacaa cccgttcgcg ataggttccc atgtatagac
     3721 gctgttcatc tggattcaat cgtgtttcgc cagaggcttt ctgtagtatg gtagtattta
     3781 aatcgttcat ggtttcagta taacatactc agttaacttt gaaaaggaag gcgtttacat
     3841 tatttctttc agtaaaataa cctgtttttt cttgaaaaat cccttctttt agggtatgat
     3901 agctatgtaa caattttaaa ctcaaaggag agtaatataa tgtcaattat tactgatgtt
     3961 tacgctcgcg aagtccttga ctcacgcggt aaccctacac ttgaagttga agtttatact
     4021 gaatcaggtg ctttcggacg tggtatggtt ccttcaggag cttctactgg tgagcacgaa
     4081 gcagttgagc ttcgcgacgg tgacaaatct cgttaccttg gtcttggtac tcaaaaagct
     4141 gttgacaacg tgaacaacgt gattgctgac gctatcatcg gttttgacgt tcgtgatcaa
     4201 caagctatcg accgcgctat gatcgctctt gacggtactc ctaacaaagg taaattgggt
     4261 gcaaacgcaa tcctcggtgt ttctatcgct gttgcgcgtg ctgctgctga ctaccttgaa
     4321 gtgccacttt acacttacct tggtggattc aacactaaag tattgccaac tccaatgatg
     4381 aacatcatca acggtggttc tcactcagac gctccaatcg ctttccaaga attcatgatc
     4441 ttgccagttg gcgctccttc attcaaagaa ggtcttcgtt ggggtgctga agtattccac
     4501 gctttgaaga aaatcttgaa agctcgtggt ttggtaacag ctgttggtga cgaaggtggt
     4561 ttcgctccta agttcgaagg aactgaagac ggtgttgaaa caatcatcga agctatcgaa
     4621 gctgctggtt acgaagctgg tgaaaacggc atcatgattg gtttcgactg tgcgtcatct
     4681 gaattctacg acaaagaacg taaagtttac gactacacta aattcgaagg tgaaggcgct
     4741 gctgttcgta catctgcaga acaaatcgac taccttgaag aattggttaa caaataccca
     4801 atcatcacta tcgaagatgg tatggatgaa aacgactggg atggctggaa agctcttact
     4861 gagcgtcttg gcaaacgcgt tcaattggtt ggtgacgact tcttcgtaac aaacactgac
     4921 taccttgcac gtggtatcaa agaaggtgct gctaactcaa tccttatcaa agttaaccaa
     4981 atcggtactc ttactgaaac atttgaagct atcgaaatgg ctaaagaagc tggctacact
     5041 gccgttgtat cacaccgttc aggtgaaact gaagattcaa caatcgctga catcgcagtt
     5101 gcaactaacg ctggccaaat caagactggt tcattgtcac gtacagaccg tatcgctaaa
     5161 tacaaccaat tgcttcgtat cgaagatcaa cttggtgaag ttgcagtcta caaaggcttg
     5221 aactcattct acaacttgaa aaaataaaac agtctgggag actgttttat ccctgagcta
     5281 gcatagcgag ggttatagta agaacaagtc tggaaatttc cagacttgtt tttcgtagta
     5341 cagtgtacta atttatcccc gagctagtat agtgagggat atagtaagaa caagtctgaa
     5401 aatattcgga cttgttcttt gactttatat gatttaaggg ctgtaaagcc ttgatataag
     5461 gcactttggg gcatcatccc aaggtacttt tttgatagat tgacagatac tttaaaaatg
     5521 tgttacaatg taacacaaag aagcgaggtg aatatcatga gtacagtgac aatcagatta
     5581 aatcaagaag aagaagtatt ctttaaaagc tatgcacaat taacaggtca aagtctttct
     5641 agccttttta agaaagcttt ggagcgtgat attgaagatg agtatgattt aaaaatctat
     5701 catcaagctt atgatgagta caaagcagat ccagaaacaa ttagtcatgc tgattttaag
     5761 aaagagctag gattatagca tgtatcattt agagtattct aaaaaggctc aaaagcaaat
     5821 tatgaaatta gataggcaaa ttcagaggct gttgtttgct tggattgata aacacttaga
     5881 agggactgat aatccacgcg ccaatggtaa aggtttgaca ggcaatcatg ctaacgagtg
     5941 gcgttatcgt atcggagatt accgactgat ttgcgatatt caagatgata agttggttat
     6001 tttagcactt gagtttggac atcgtaaaga tgtctattga tgcgtgaaaa tctatgacaa
     6061 gataggtcct aactattttt aagaatgaat agaaaactag actgaaattc ctagggaatt
     6121 ttctttgcct tgatattgat aaaatgatat tctaaaaagg cagatcaact taagtattct
     6181 cattttgaaa agagatggag agtgggatga tagagtggat attttttgat ttgggttcga
     6241 cgcttttaga tgaagaggct gcttatggtt attatataga caagtgtgtg aagaagttag
     6301 agtctctgga tatagaggtt tcatcagatt cttataaaaa gaaaatggtg gagtatgctc
     6361 ataagtcgct ggatcccatt cgttcgacct ggcactattt tgcaccgacc gagccacgtc
     6421 ctttgtggac aaatgagggt gtgagcttgt atcctgaaac aatagatgct ctagagaagt
     6481 tatcacaaaa ctatcgattg gggattattg ccaatcagtc tagtagtatt agagaactgc
     6541 ttaaagagtg gggaattgag tcttattttc aactcatcat cctctctgaa gaggttgggc
     6601 tatctaaacc aaatacagct attttcacac ttgccttgca aaaaacaaac atccctgcag
     6661 atagagttgt ctatgtcggg gataggtttg ataatgatat tctgcctgct aaatctttgg
     6721 gaatgtggac tgtacggata ctgacaggtt ttgggaagca tgccagtgaa aatgaaaaat
     6781 tgaagagcga ttggataatt ccttcattgc aagagataac aaatattttt gaacaaacaa
     6841 aaaactagac tgaacattct cagcctagtt tttacatgta ttagaacaag cctttaactt
     6901 tatctaaaag accgttaaca ccttcagaac ctgataacaa gccttttgct tgttccaagt
     6961 attcaccgaa ttggtccttg ttttcttcga taaattgttt tgcaccatca aagtctttgt
     7021 ttgcaatcaa ttcttttact tggttaaaaa gatctactgg attcatgttg aaatcacctc
     7081 ctatgagact attaaaccaa aactgtacgg atttcacaac tgaaacgact tataaaaatg
     7141 taacgcaaac tgcttcagga acatagaaat cgtgtgaaag ctcagtcaaa cagccatttt
     7201 cggcatctct tttgaataca gttgcattgt cagaatcttg atggacaaca atcaaatgct
     7261 tttcatcagg actaagcgtg aaatcgcgag gagtttttcc atttgttggg acgatttcaa
     7321 ctaattctaa gctggcatca gcaagcgttt tgtagactgc gatagagtcg tttccgcggt
     7381 tggatccata aataaattta ccatcctttg tgatgcgaat agcggcagta ccgttgaaac
     7441 cagtatggtc ttctgggaga gtagagacag tttgtaggtg ttcaaattgt ccccagccgt
     7501 cgtagataag cacctcaata gttgaattga gttcacagat gagataggcg atttttgcga
     7561 cagtatggaa gacaatgtga cgtggaccac ttcctggcgc agcctgatag gttgctattt
     7621 tctctacttt tccttggtca gataccttat aggttaccac ttgatcacat cctaaatcac
     7681 aggttactaa aaatttatcg ggcgtcaaat cagaaaagtg aacgtgggcg gaagcttggt
     7741 tttcgtgcgg cccagatcct tcgtgttgag cgacatcagc taattctagc gaaccatccg
     7801 ccaaacgttt ataaacaagt agttgccctt tgtggaaatt ggaaccataa actaaatctc
     7861 ttgcttcatc tacagcgaca tagcaaagtg gtgcgccctc tgatacaaca tggttgagta
     7921 gagtatagtc agtattaaag gcagcgatac ctcctagacc attttcagcg ccgacggagt
     7981 atagatgtcc ggccttatca aaggcaaggt aggtagggtt aggttcttca gtgaccaatt
     8041 ctagattgct aagttgtcct gtttcagagt caaaatttgc tttataaatt ccttttgatt
     8101 cacgttttgt ataggtaccg aaatagatag ccataggatt ctcctttttg gaaatgattg
     8161 ataaaattat accatgtttt tctgagaaag tcgtatagat tctgtagttg atttttacaa
     8221 aaatcctttc tccatgttac aatagaaaaa aagactagga gtgtatcaga tggaggataa
     8281 gcatcaaaaa tttagtctga ctttgttttt ccgcttgttt ttaaatagcc aagcggtgac
     8341 atttttattg gtaaccttgc tgactttttt gaccatattt atttttagta aaattagttt
     8401 tttattcaga ccaataggaa gctttttaga aattgtattg ttaccgatga ttttaacagg
     8461 tcttttgtat tacctattaa atccgatggt tgactggatg gaaaagcata aaatttctcg
     8521 tacagttggt atttccattc tctttgtttt aatcagtctt ctcattatct ggggactggc
     8581 ggtggcaatt ccgagtattc aagagcaagt gacatctttt gcgcaaaatc ttccttcgaa
     8641 tatccagaaa atcgaaggac aggtaactgg gctcctacag gatcagcgct ttgaacaatt
     8701 tagaccaaca gctctggaga tgttaaataa ggtcaatgat cagatcgttg cctatgctca
     8761 gaagttttct tcaagcgctg tgaactgggc aagtaatttg atttcaaccg cttcacagat
     8821 tattgttgct attttgatta tgccctttat tctcttttat ctattacggg atggtcagta
     8881 tttgaataaa cacatcaccc aatatctacc aaccaagtgg agagaaccga ttggaacagt
     8941 actatcagat gtgaatgggc aattatcaaa ctatgttcgt ggtcaggtga ccgttgcgat
     9001 tattgttgcc ctgatgtttt cagtgatgtt ctcaatcatt ggtcttagct atccaataac
     9061 tttgggggtg atggctggtt tcctcaatct aattccgtat ttgggttctt tcttggccat
     9121 gattccagct gttattttag gattgattgc tggtccaatc atgctgataa aggttttagt
     9181 agtctttatg attgagcaaa ctatagaagg tcgttttgtg acaccattga ttatcggaag
     9241 ctccctaagt attcatccga ttaccatttt gtttgtatta ttgacagctg gtcagatgta
     9301 tggtgtccta ggagttcttc tgggaattcc aatatatgcc tctattaagg ttttagtgaa
     9361 agcggcattt gaatggtaca aggcacacag tggtttgtat gaagatgaag aaaatgaggt
     9421 tgtgaatgaa caatagtgaa aaaatgttgg tttgtttgga ccaacaggat ttagacaagg
     9481 cagataagta tttcaagcgc gccttggtgc aagatgatag cgaaacgctt ttgtccttgg
     9541 cagcctactt agaaggcatt ggttttttcc cacaggctag acaaatctat gagcaggtca
     9601 aagaagaatt tcccgaagta ctgatcaatc ttgcccaaat agcatttgaa gatggcttgg
     9661 ttgaagaatc ttttggatac ttggaggaaa tttcggaaga cagttctgtc tatgtggaag
     9721 ccttgttggt taaggcggac ctgtatcagg cagaaggctt gtcggatgtg gcgcgtgaaa
     9781 aattgttaga agcaagtcac ttatcagatg aacccatcat cctctttggc ttagcagaat
     9841 tggatatgga actggagctt tttaatgagg ctatttccta ctatgcccag ctggataatc
     9901 gtgagatcta cgaattaacg ggagtttcta cctatcagcg aattggtatt gcctatgcaa
     9961 gtttggggaa atttgaagcg gcgattgaat ttttggaaaa agctgttgag ttggaatacg
    10021 atgatcagac ggtttttgag ttagcagctc tgctatttga acgagaagaa taccaaaaag
    10081 caaatctcta tttcaagcag ttggatacca tcaatcctga ttttgagggc tatgaatatg
    10141 cctatgctca gtcgcttcat gtcgagcata agattgatga tgccttggtt atgactcaaa
    10201 aaggcctggc taaaaatgat tttgacgcca atctcttgct ccaggcttct cagtatgcct
    10261 atgaattgca tgatgaggcg ggggcagaag attatcttct acgggcaaaa gaggtagcag
    10321 atgatagtaa cgaactagct ctgcgcttgt ccaatctgta tcttgagcag gaacgatttg
    10381 aagaggttgt ggctttattt gatgaagagg tagataacgt tctagctcgc tggaatgtgg
    10441 ccaaggctta tcaagctcta gaaagagacg atgaagctat tgagctttac gatgaattgg
    10501 ctggagaatt tgctgagaat cctgaatttt tggctgatta cgtagcggtt ttacgtcaac
    10561 ttggtcgttt ggatgaggca aaagagcaag ctagtcgtta tatccaattg gtgccagacg
    10621 atttagccat gcaagaattt ttgaatgaag agtaggttat gcaagtaaat cgattatttc
    10681 aatacaatac cttaggtgcc ttaatggctg gactgtatgg cggctccttg acggttggcg
    10741 aattgttgga acatggtgat ttgggcttgg gaaccctgga ttccattgat ggcgagctga
    10801 ttgtcttgga tggaaaggct tatcaggcca agggagcagg ggagaaacca gaagtggttg
    10861 aagttcctgc tgagatgaag gttccttatg cagcggtcat tttccatgaa gcagaagtga
    10921 tttttaagca acgctttgaa atgaccagcg atgaattgca tcagcggatt gaatcctatt
    10981 atgatgggga aaatcttttt cgctccatca aaatcaaagg gacgttttca cggatgcatg
    11041 tccgcatgat tccaaaatcg gcttctgatg ttcgttttgc ggaggtggca agtcgtcagc
    11101 ctgagtatac cgctgagaat atttcaggta ccattgttgg tatttggaca ccagaaattt
    11161 ttcacggggt cagtgtggct ggctatcatt tgcattttat ttcggatgat ttgacatttg
    11221 gcgggcatgt catggattat gttatctcag aaggaatggt ggaagtgggg ccagtagatc
    11281 aactagacca acgcttccca gtccaagacc gccaatatct ttttgctaag ttcaatgcta
    11341 aggaagttag agaagatatt gataaggcgg agtaggcttc aatacagaaa aatccatgaa
    11401 ctagattttc atggatttta ttttatcttc agttggggtg acacggaggg tcttctgacc
    11461 tcggtaggtc acaaagcctg gtttgccacc tgttggctta tggagttttt tggcttcaat
    11521 catgtcgact tggaccagat tggaatgtct ggccttggag aagtaggcag ctaattcagc
    11581 tgcgtctgtc ttgacctcgt cacttgggtc caagttatct gtgatgacca catgactacc
    11641 aggaatgtct ttggcatgga accagagttc gccttttttg gccattttga aggtcaactc
    11701 atcattttgc aggttatttt ttcctaccag aatgatggtt ttcccgtctg ttgccaagta
    11761 acgttcaggt ttctgacgct tatggatttt ctcacgatga cggcgtttga ggtagcctgt
    11821 ttcaatcaat tcctcacgga tttcatcgat ttctgccaga ctagcctgtc ccagcatggt
    11881 gtcaacggac tccagataga caatggtcga cttggtctcc tcaatcaagt tggtcaggtg
    11941 cttgaccgcc tccttgagtt tctggtactt cttgaagtac cgctgggcat tttggctagg
    12001 agtcaaagcc acatccaact caatctccag ttccttgccc gtatagtagt tgtctaaccg
    12061 cacactcgac tggtcatttg gcacttgatg cagataggtg gtcaggagct cgcccttttg
    12121 ccgcaccaac tccgccgtct ccgtatccgc caattcctgc tcctgcttga ttagtttctt
    12181 gcgatttttc tccaactcac tggctacccg cttgatcagc tcattggcct gctgggccac
    12241 ccgatcccgc tccgccttat cctgatagta gaagtccagc agactgctca ggctctcaaa
    12301 gtgtggctgg ttttcagggc tattggcaaa tggcagagca gcataggatt tgtctgtcag
    12361 gctaggctga gttgcctggt caaaaaaggc acggaagttc ttcaggcggt ctatctgcaa
    12421 atgattggcc agttcagaag ccgtatcccg acccaaacct tgaaagacct gttggagatt
    12481 ttttggactc agttcctgag tcgataagat ttcaaacaat ttctcgtcag acaccgtata
    12541 aggattgaga gaatgcgtct ccggtggacg aatataggtg gagcctggca agatggttcg
    12601 gtaggaattt tgcgagaaac caacgtgctt gatagcctca ataatcttct gttcagactt
    12661 atctaccaag atgatattgc tgtgcttgcc catgatttcc acaatcaagg ttgcttggat
    12721 atggtcgcca atctcgtcct tgttggacac cgaaaattct aagatacggt cattgtccaa
    12781 ctgacgaatc tcctcgataa tggctccctg caagtatttc cgcagaatca tggtaaaggt
    12841 attgggcact tttggattgg taaagtccga ctgggtcagc tgaacccgac cgaaaaccga
    12901 atgggcggac agaagcaatt tatggctctg acggttgctg cggatgttga ggacaatctc
    12961 ttgttcaaag ggctggttga ttttctgaat ccgcccacct tctaaattgg cccttaactc
    13021 agccgtcatg tgatgtaaaa aaaatccgtc aaaagacata gttttcctct ttctaatagc
    13081 tataactcag tctagtatat cataaatcag tgacttttt
//
LOCUS       DBNFCK010000049        13098 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.049, whole genome shotgun sequence.
ACCESSION   DBNFCK010000049 DBNFCK010000000
VERSION     DBNFCK010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13098
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.049"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..512)
                     /locus_tag="AC1PIW_001105"
     CDS             complement(<1..512)
                     /locus_tag="AC1PIW_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HIP6606048.1"
                     /translation="MFKKYTFDPKKFRLGMRTFKSGLAVFLVILLFGFMGWQGIQIAA
                     LTAVFSLREDFDQSVHFGASRILGNSIGGFYALLFFILDRLFLDHFWVTAVFVPIFVM
                     LTIMTNVAMNNKAGIIGGVSALLIITLSIPAGDTIQYVFIRVFETFIGVFIAILVNYD
                     VNVIKKRFQD"
     gene            complement(1319..2518)
                     /locus_tag="AC1PIW_001106"
     CDS             complement(1319..2518)
                     /locus_tag="AC1PIW_001106"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIP6606049.1"
                     /translation="MAKLTVKDVELKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYILEQGGRAILFSHLGRVKEEADKEGKSLAPVAADLAAKLGQDVAFIAGATRGAELE
                     AAINALEDGQVLLVENTRFEDVDGKKESKNDEELGKYWASLGDGIFVNDAFGTAHRSH
                     ASNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLE
                     KADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKTLLEKANGKLILPVDSKEANA
                     FAGYTEVRDTDGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQA
                     GTIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPG
                     LAALTEK"
     gene            complement(2774..3784)
                     /gene="gap"
                     /locus_tag="AC1PIW_001107"
     CDS             complement(2774..3784)
                     /gene="gap"
                     /locus_tag="AC1PIW_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIP6606050.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKDGGFEVNGKFVKVSAEREPGNIDWATDGVDIVLEATGFFAS
                     KEKAEQHIHANGAKKVVVTAPGGNDVKTVVFNTNHDILDGTETVISGASCTTNCLAPM
                     AKALHDAFGVQKGLMTTIHGYTGDQMVLDGPHRGGDLRRARAAAANIVPNSTGAAKAI
                     GLVIPELNGKLDGAAQRVPVPTGSVTELVATLDKKVTAEEVNAAMKAAATESFGYTED
                     QLVSSDIVGISFGSLFDATQTKVIEVDGEQLVKVVSWYDNEMSYTAQLVRTLEYFAKI
                     AK"
     gene            complement(4151..6142)
                     /locus_tag="AC1PIW_001108"
     CDS             complement(4151..6142)
                     /locus_tag="AC1PIW_001108"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HIP6606051.1"
                     /translation="MRKKLKFSLVAVACATFLTACGTSNSSAPKAGEATPQTDFHSYI
                     NAQWLKNTKLGAGESQIDNLSQLSEKTQEAIQEMIDQLDMDYSKLQAGSDEKKLIDFY
                     RLAADFDTRNKLGLEPIKPYLEEIKKASTMEELNKILVALYGKNIRNLLHINVTQDIK
                     DSNKNTIYVDPHELSFPKENYDGTDEFSLKNQKAFKAYLQEVFSLSGETSKEASSKAD
                     LLFNLEKEMAAVQRPKEEANNFEAMYNEKTWDEVKALVPNLPIAELVSELGLQNAPTT
                     VVSDPDALKNLNELYQEKNLDALKALLQYRVIAHYNAYLSSNLIEAKAKYDGVATGAV
                     DTPSQKEVAQAAIDANFSDMIGKVYVKHHFSEESKANILSMVQEIKATYAERIKAVQW
                     MSEETKMRALKKLDTLVVKIGYPDQWKDYSQLAIQSKEEGGSLLSNFDMVKTLELREE
                     MGQFGKPFDKSQFGMPSHTVNAYYSPTNNEIVFPAGILQAPLYDPKASPEENLGGIGA
                     VIGHEISHAFDKAGSQFDEKGNLVNWWKPEDLEKFNKKVQQAADIYSKLEVAPGYYVN
                     GEISTGEIMADLGGLTVAIDIAQKKGYDTKKVFESYGKAWREVTTKEFAIANISDEHP
                     PAKYRVNNIVNQIDQFYTDFNVKEGDPMYVKPEDRLRVW"
     gene            complement(7131..9212)
                     /gene="fusA"
                     /locus_tag="AC1PIW_001109"
     CDS             complement(7131..9212)
                     /gene="fusA"
                     /locus_tag="AC1PIW_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIP6606052.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHERLQANAH
                     PIQLPIGSEDEFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLEQAEEYREKLVEAV
                     AETDEELMMKYLEGEEITNDELKAAIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGVNPDTDAEEERHASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     NSGSYVLNTSKGKRERIGRILQMHANSRQEIETVYAGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESIHVPEPVIQLMVEPKSKADQDKMGIALSKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPIVDVKAKLY
                     DGSYHDVDSSETAFKVAASLALKEAAKSAQPTILEPMMLVTITAPEDNLGDVMGHVTA
                     RRGRVDGMEARGNTQIVRAYVPLAEMFGYATVLRSATQGRGTFMMVFDHYEDVPKSVQ
                     DEIIKKNGGNA"
     gene            complement(9769..10239)
                     /gene="rpsG"
                     /locus_tag="AC1PIW_001110"
     CDS             complement(9769..10239)
                     /gene="rpsG"
                     /locus_tag="AC1PIW_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HIP6606053.1"
                     /translation="MSRKNQAPKREVLPDPLYNSKLVTRLINRVMLDGKRGTAASIVY
                     GAFDQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARNRGEHTMIDRLAKEIMDAANNTGAAVKKREDTHKMAEANRAFAHFRW"
     gene            complement(10256..10669)
                     /gene="rpsL"
                     /locus_tag="AC1PIW_001111"
     CDS             complement(10256..10669)
                     /gene="rpsL"
                     /locus_tag="AC1PIW_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HIP6606054.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSRKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKG"
     gene            complement(10901..12523)
                     /gene="groL"
                     /locus_tag="AC1PIW_001112"
     CDS             complement(10901..12523)
                     /gene="groL"
                     /locus_tag="AC1PIW_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940029.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIP6606055.1"
                     /translation="MAKEIKFAADARESMVRGVDILADTVKVTLGPKGRNVVLEKAYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPIGIRRGIEAAVATAVEALKAQASPVSNKAEIAQVAAVSSRSEKVGEYI
                     SEAMERVGTDGVITIEESRGMETELDVVEGMQFDRGYLSQYMVTDNEKMVAELENPFI
                     LITDKKISHIQDILPLLESILQANRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLDLKDATIEALGQAAKVVVDKDGATIVEGA
                     GNPEAIANRVAVIKSQIEVTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALVNVIDSVAKLELKGDDETGRNIVLRALEEPVRQ
                     IAYNAGYEGSVIIDKLKNSELGTGFNAATGEWVNMMDAGIIDPVKVTRSALQNAASVA
                     SLILTTEAVVANKPEPAAPAMPQGMDGMGMGY"
     gene            complement(12534..12815)
                     /gene="groES"
                     /locus_tag="AC1PIW_001113"
     CDS             complement(12534..12815)
                     /gene="groES"
                     /locus_tag="AC1PIW_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIP6606056.1"
                     /translation="MLKPLGDRIAVKIEEKEQTVGGFVLAGASQEKTKEASVLAIGQG
                     IRTLNGDLVAPAVAVGDTVLIDAHAGLEVKDGDQNVHIIRETDILAIVE"
BASE COUNT         3624 a         2948 c         2504 g         4022 t
ORIGIN      
        1 ttatcctgaa aacgtttttt tataacattc acatcataat taactagaat cgcaatgaaa
       61 actccgataa aggtttcaaa cacacgaata aacacatatt gaatcgtatc accagcagga
      121 atagacaatg taataattaa taaagctgat actccaccaa taattcccgc tttattattc
      181 atcgccacat ttgtcataat cgttaacatg acaaatatcg gaacaaaaac cgcagttacc
      241 caaaaatgat ctaagaataa cctatccaaa ataaagaaaa gtagcgcata aaaaccacca
      301 atagaattgc ccagaatccg agaagctcca aaatggacac tctgatcgaa gtcttctcgt
      361 aaactaaaca cagccgtcaa agccgcaatc tgaatccctt gccagcccat aaaaccaaac
      421 aaaagaataa ctaggaaaac tgccaaaccc gatttgaacg tccgcatccc caaccgaaat
      481 ttctttggat caaatgtata ttttttaaac atgcctctat tctaacatga tttcggaaaa
      541 tagaatagca aatacctgcc cagcttttct tttctcctaa gctcgactgg aaaagcctcc
      601 tgttcacgcc agcttttcga aaaaccaata atctgccact ttaaatcagc tgcaagaagg
      661 aacccagaag taagacaaac aaatagacgc tgcaaaaaac aacgtctttt tttacattta
      721 tatttttagc gcagtagctg aatgggagtt gtgaccaaga ttcagccttg cggggttcaa
      781 atagcccagt ggactatttg aagttgtcac caagaaaaat caaagtaaca atctacggaa
      841 tcatttttga cagaagcttc gtttcttcta tttctaacct caacatatct gggagatatg
      901 ttgaactgac tcaccgccta tttctcagtc aaggcagcac ctagccagtc tttctaggtc
      961 acacttccca gaaagactag gcatggcact aggcactccg tgccgtcgca agtcctggaa
     1021 gcactttaca ccttgctaga ctttctaact cgcttagctt cgtagaaact ctacgcgatg
     1081 cacaaaatgc aacggaaatc cgttgcattt tagcctttac ctaggctgat tttctaaact
     1141 cactacgttc acagaaaatc tagcctttgt actagaaaag agttggtctc ccaactcttt
     1201 tcatcgttca tctttcatac gcagtagctg aatggcagtt cactttgcta cgcttgtgac
     1261 caacctattc agccttgcgg gggtgcgtct acggaatcat agattcctga cttaccgcct
     1321 atttttcagt caaagctgca agtcctggaa gcactttacc ttcgaggagt tccattgatg
     1381 caccaccacc agttgagatc catgagaact tgtctgcacg gccaaggttg atcgcagctg
     1441 ccgctgagtc accaccaccg ataattgatt tcacgcctgg ttgtttcacg atagcgtcca
     1501 ttacaccgat tgtaccagct tggaagtctg ggttttcaaa tacacccata ggtccgttcc
     1561 aaacaactgt tttcgcacca gtcaaagctt cgtcaaactt agcgattgac ttaggaccga
     1621 tgtcaagacc aaggaagcct tctgaaactg cttcgccgtc tgtatcgcga acttcagtgt
     1681 atccagcaaa tgcgtttgct tcttttgagt caactggcaa gattaattta ccatttgctt
     1741 tttcaagaag tgtttttgcc acatcaagct tgtcttcttc tacaagtgag ttaccgattt
     1801 cgataccttg agctttgtag aatgtgtaag tcataccacc accgataaga actttgtcag
     1861 ctttttcaag aaggttctcg ataacaccga tcttatcaga tacttttgaa ccgccaagga
     1921 ttgccacgaa tgggcgctct ggagtttcaa cagcttcttg gatgtaagca atttcgtttt
     1981 ccaaaaggaa accagctact gctttttcta cgtttgctga gataccaacg tttgatgcgt
     2041 gtgaacggtg tgcagtacca aatgcatcgt taacgaagat accatcacca agtgaagccc
     2101 agtatttacc aagttcttcg tcgtttttag attctttctt accatcaaca tcttcgaaac
     2161 gagtgttttc aacaaggaga acttgtccat cttccaaagc attgatagct gcttccaatt
     2221 cagcaccacg agtagcacct gcgatgaaag caacgtcttg acccaattta gccgccaagt
     2281 cagctgctac aggagccaat gatttacctt ctttgtcagc ttcttctttc acacgaccaa
     2341 ggtgagagaa aagaattgca cgtccacctt gctcaagaat atacttgata gttggaagag
     2401 ctgctgtaat acggttatcg ttagtgataa cgccatcttt caaaggcacg ttgaagtcca
     2461 cacgaacaag aacttttttg cctttcaatt ctacatcttt aacagtcaat tttgccatta
     2521 gataagaact ccttaaatta tatttacttt accattgtat catattttct gtattttttc
     2581 ttgcataaag atattttttt cacaaatgtt atgaaatcta aaaactatcg cctcgtaatc
     2641 agatttttca aattctaaag ttgccaaatc agaaacaact tataccatcc ttgtttctta
     2701 gcataaggaa acagctatat ttcagcaatc aaaacaaaaa agaggccgaa gcctcttcga
     2761 ttttcactaa ggattattta gcgatttttg cgaagtactc aagagtacga acaagttgtg
     2821 cagtgtaaga catttcgttg tcgtaccatg aaacaacttt aaccaattgc tcgccatcaa
     2881 cttcgataac tttagtttga gttgcatcaa acaatgaacc gaatgagata cctacgatat
     2941 ctgaagatac gagttggtct tcagtgtaac caaatgattc agtagcagca gctttcatag
     3001 cagcgtttac ttcttcagca gttactttct tgtcaagagt tgcaaccaat tcagttacag
     3061 aacctgttgg aactggaaca cgttgtgcag caccgtcaag tttaccgttc aattctggga
     3121 ttaccaagcc gatagcttta gcagcaccag ttgagttagg aacgatgttt gcagcagcag
     3181 cacgtgcacg acgaaggtca ccaccacggt gtggtccgtc aagaaccatt tggtcaccag
     3241 tgtaaccgtg gattgtagtc atcaaacctt tttgaacgcc aaatgcatcg tgaagagctt
     3301 tagccattgg tgccaaacag tttgtagtac atgaagcacc tgagataact gtttcagtac
     3361 catcaaggat gtcgtggtta gtgttgaaaa caacagtctt aacatcgtta ccaccaggag
     3421 cagtgacaac aactttttta gcaccgttag cgtggatgtg ttgctcagct ttttctttag
     3481 aagcaaagaa acctgtagct tccaaaacga tatctacgcc atcagtagcc cagtcgatgt
     3541 ttcctggctc gcgctctgca gaaactttaa cgaatttacc gttaacttcg aaaccaccgt
     3601 ctttaacttc aacagtaccg tcgaaacgac cttgagttgt gtcatatttc aacaagtgtg
     3661 caagcattac tggatctgta aggtcgttga tacgagtaac ttcaacacct tctacgtttt
     3721 ggatacgacg gaaagcaaga cgaccgatac gtccgaaacc gttaatacca actttaacta
     3781 ccatgaatga tttcctcctt atgaaaatcg agttctttat tttaaaaggc aagcctttta
     3841 atccttttgt gaaaatatta acttgtaagc ctacaagcat cttttcaacg tttttattat
     3901 ataactattt accgaaaatt gcaactgctt accggttcat cttatgtaaa aaatagaagg
     3961 ccaaaaaata aaagttaaaa atcatagcca tgcgttttct cgggtggtta tgactggtta
     4021 aatacttaac ttaatagcat tgccatacct cgacacattt tttaccgatt cttggtttac
     4081 cgctacttct attttttacc taaaaaggtg tctaccgccc attttggacc gcagacacct
     4141 ctataatctt ttaccacact cgtaggcggt cttctggttt cacatacatt ggatcccctt
     4201 ctttgacatt gaagtctgta tagaattgat caatttggtt gacaatgttg ttaacccgat
     4261 atttagctgg tggatgttcg tcgctgatgt tggcaatagc aaattctttg gtggtgactt
     4321 cacgccatgc tttaccataa gactcgaaga ctttcttcgt atcgtagcct tttttctgag
     4381 caatatcaat ggcaaccgtt aaaccaccga gatccgccat gatttcaccg gttgaaattt
     4441 cgccattgac atagtagccc ggagccactt ccaatttaga gtagatatct gctgcctgtt
     4501 gaaccttttt attaaatttt tcaaggtctt ctggtttcca ccagttgaca aggttcccct
     4561 tttcatcaaa ttgagaacca gccttatcaa aggcatggga aatttcatga ccgataacag
     4621 ccccgattcc acctagattt tcttcgggac tagcttttgg atcgtagaga ggagcctgta
     4681 aaatccccgc tggaaagaca atttcgttgt tagttgggct gtaataggca ttcaccgtgt
     4741 gagaaggcat gccgaactgc gacttatcaa aaggcttgcc aaattgcccc atctcttcac
     4801 gcaattcaag tgtttttacc atatcgaagt tagaaagaag actcccccct tcttccttgg
     4861 attggatagc aagttgtgag taatccttcc actggtctgg atagccgatt ttaacaacca
     4921 atgtatctag ttttttcaag gcccgcatct tggtttcttc agacatccac tgcactgctt
     4981 tgattcgctc tgcataagtc gctttaatct cttgaaccat agataggata ttagccttgg
     5041 attcttctga aaaatggtgt ttaacgtaaa ctttgccaat catatcggag aaatttgcat
     5101 caatggccgc ttgagcaact tctttctgac tcggagtatc taccgcaccg gttgctacgc
     5161 cgtcgtattt cgcttttgcc tcgattaaat tgcttgacag ataagcattg taatgggcaa
     5221 tcacacgata ttggagcaga gccttaagag cgtctaaatt cttttcttga tagagttcat
     5281 ttaaattttt tagagcatct ggatctgata cgactgtcgt tggggcattc tgaagtccta
     5341 actcagaaac caactcagca atcggtaggt tgggaaccag tgctttgacc tcatcccaag
     5401 tcttctcgtt atacatggct tcaaagttat tggcttcttc ttttggccgt tgcactgcag
     5461 ccatctcttt ttccaaatta aagaggagat ctgccttaga agaggcttct tttgaggttt
     5521 ctcctgacaa gctaaatact tcctgcagat aggctttaaa ggctttttga tttttcaatg
     5581 aaaattcatc tgtgccatca taattttctt ttggaaaact taactcgtga gggtcaacgt
     5641 agatggtgtt tttgttactg tcttttatat cttgagtaac gtttatatga agcaaattgc
     5701 ggatgttttt gccgtatagg gccaccagaa tcttgttgag ttcttccatg gtagaggctt
     5761 ttttaatctc ctctagataa ggcttaatag gctctaaccc caatttgttg cgagtatcaa
     5821 aatcggctgc caatcggtaa aaatcaataa gttttttctc atcactacca gcttgcagct
     5881 tggaatagtc catatccagc tggtcaatca tctcctgtat cgcttcttgc gttttctcag
     5941 agagttgact cagattatct atttgcgatt caccggcacc taattttgta tttttcaacc
     6001 attgcgcatt gatataagaa tgaaaatccg tctgtggtgt tgcttcacct gctttgggag
     6061 ccgaggaatt acttgtgcca caggctgtca aaaatgtcgc acaggcgact gctactaatg
     6121 aaaattttag tttttttctc atggtttctg tcctttcttt ttattgttaa tgtgcttagt
     6181 accactattt catcaccata caagcatcct cctttagtaa ttagcggtcc taaactactc
     6241 tttcatcgat ctaccttata aggattctaa catttatatc tgcttttctt ctgtagccta
     6301 tctattaatt ctcttatttt ttataaccat tcccaaacaa agtaattcta aataatttaa
     6361 aacatttatt ttagctgatt aagtgtgagt aggcgagaac tcgaacgatc aaaagagttc
     6421 ggcaacaatt cctaatttct agttgttgag ctgaaatagt ctaaccccag actgttttaa
     6481 tccgtttatt tcacagtagt cggacctggg agtcactgtt gagttcatcg acaacaggaa
     6541 cttataccca atgaaatccc aaaatagcaa accctgatct tttgttagcc atccacacct
     6601 aaattagggt aattttttat aaattgtagc tttctcattg tatatttcca aaatgaatct
     6661 ggcggatatc tgttttatat agtgtagtcg tttagtttgt ttttagtact cgcttcaaag
     6721 gttcgggaaa cctttgaagg ctggagatag ggcgaagcaa gttcgtctca atagctgcaa
     6781 gttgctagaa cagcctaaag gctgttctag gttggaaata agacttgcct cgcaaatcac
     6841 ttctgtaaag tacgacaaag cgagttcaaa tactccagtg aagtgtttga agttggaaat
     6901 aaggaaacga agtttcctcg ctcgtcgaag caatcaaaga taaactactg atactcaatg
     6961 aaaatcaaaa gtagcctagg aaacgaagcc gaagatagaa ctggagttca tcaaggcaag
     7021 ttgacaacgg ataattttga ttttcgaaga gtatgagtat cacgctcaga tgtgtgacat
     7081 atccgtcgta ccaaaaaaac acctacccga aggtaggtgt agatagacaa ttatgcattg
     7141 ccaccgtttt tcttaatgat ttcatcttgt acagattttg gtacatcttc gtagtggtca
     7201 aataccatca tgaaagtacc gcgaccttgt gttgctgaac gaagaacagt tgcgtaacca
     7261 aacatttcag caagtggtac ataagcacga acaatctgtg tgttaccacg agcttccata
     7321 ccatcaacgc gaccacgacg tgcagtaacg tgacccataa cgtcacctag gttatcttca
     7381 ggtgcagtga ttgttacaag catcattggc tcaaggatag ttggttgagc tgatttagcc
     7441 gcttctttaa gggcaagaga tgcagccact ttgaaggctg tttcagatga gtcgacatcg
     7501 tggtatgaac catcgtaaag cttagcttta acgtcaacaa ttgggtaacc agcaagaaca
     7561 ccgttagcca tagattctac gagacctttt tcaaccgctg ggatgaattc acgtggaacc
     7621 acaccaccga cgatagcgtt ctcgaactcg aaacctttac cttcttcgtt tggtgtgaat
     7681 tcgatccaaa cgtcaccgaa ttgaccttta ccaccagact ggcgtttgaa gaaaccacga
     7741 gcttgtgtag aagcgcggaa tgtttcacgg tatgatactt gaggagcacc tacgtttgct
     7801 tcaaccttga attcacgacg catacggtca acaaggacat ccaagtgcaa ctcacccata
     7861 ccagagataa ctgtttcacc agtttcaacg tttgtttcaa cgcggaatgt tggatcttct
     7921 tcagccaatt tagaaagggc aatacccatc ttgtcttggt cagctttaga tttaggctca
     7981 accatcaatt ggataactgg ttctggaacg tggattgact caaggatgat ttttgctttt
     8041 tcatctgtca atgagtcacc agttgtagta tctttcaaac caaccgcagc ggcgatgtca
     8101 cctgcataaa cagtttcaat ttcttgacgg ctgtttgcgt gcatttgaag gatacgtcca
     8161 atacgctcac gtttaccttt agaagtgttc aatacgtatg aaccgctgtt aaggacacct
     8221 gagtatacac ggaagaatgt caaacgacct acgaatgggt cagtcatgat cttgaaggca
     8281 agagctgcaa atggctcctc atctgatgca tgacgctctt cttcagcatc tgtatctggg
     8341 ttaacacctt taatagcagg gatgtcaagt gggcttggaa ggtagtcgat aaccgcatca
     8401 agcatcaact gaacaccttt gtttttgaag gctgaaccac acaatactgg gaagaattca
     8461 acgttgatag ttgctttacg gatagcagct ttcaattcgt catttgtgat ttcttcacct
     8521 tcaaggtatt tcatcatcaa ttcttcatca gtttcagcaa ctgcttcaac caatttttca
     8581 cggtattctt cagcttgctc aaggtattca gctggaatat cttcttcaag aatatctgta
     8641 ccaaggtcgt tagtatagat ttcagctttc atcttgatca agtcgatgat accacggaac
     8701 tcatcttcag aaccgattgg caattggatt ggatgagcat ttgcttgaag acgctcatgg
     8761 agtgtgctta ctgagtaaag gaagtcagca ccgattttat ccattttgtt agcgaatacg
     8821 atacgtggaa caccgtattc agttgcttga cgccatactg tttcagtttg tggctcaaca
     8881 cctgactgtg agtcaagaac ggttaccgca ccatcaagaa cgcgaagtga acgttgcact
     8941 tcgattgtga agtccacgtg tcctggtgtg tcgatgatgt ttacacggtg gttgttccat
     9001 tgagctgttg tcgcagcaga tgtgatagtg ataccacgct cttgctcttg ctccatccag
     9061 tccatttgcg atgcaccttc gtgagtttca ccgattttgt ggattttacc agtgtagtaa
     9121 agaatacgct cagttgtcgt tgttttaccc gcgtcaacgt gcgccatgat accgatatta
     9181 cgagtttttt ctaatgaaaa ttcgcgtgcc atgaggtttg ttctcctata tttttagatt
     9241 tctattctga ttataccata ttttaagaaa agcgagtggg caaatcccac ccgcttaagt
     9301 tcttactaat ggtgtgaagt ggacgaggca agcctcaatc cgatttgtga tggttgaacg
     9361 ataagttgct accataatct caattgattt atgtagaact gatttaacgt cctcacatct
     9421 taattagttg agttcgaact aaaaattctg gaaaaaagat aaatcttcct tggaccttaa
     9481 ggtccatcgt ctgatttcct atttttcctt gaattttctt aacgtcctta catcctaatt
     9541 gaactcgggc tacaactcgc tgacaaaaag atgaacctca ttgattgcac agcaatctgc
     9601 ttcgcttccc tattttgtct aggagttgct tgacgccctc gtatcttaat cttagttgaa
     9661 ttttagctac aaatgtctgc caaaaagata aaatctccta gaaactgaag tttctgcgtc
     9721 aattttccta ttttggctag catttgttta acgctaaaat atcctatctt accagcggaa
     9781 gtgtgcgaat gcgcggtttg cttctgccat tttgtgagta tcttcacgtt tcttaacagc
     9841 tgcacctgtg ttgtttgctg catccatgat ttctttcgca aggcgatcga tcatagtgtg
     9901 ctcaccacgg ttacgagcga ttgttaccaa ccaacgaaga ccaagtgttg tacgacgctc
     9961 tggacgaact tcaactggga cttggtagtt agaaccaccg acacgacgtg cacgtacttc
    10021 aagtacaggc atgatgtttt ccattgctgt ttcaaatact tcaagtgcat cgttaccagt
    10081 tgcttctttg atttgatcaa aggcaccgta aacgattgat gcagcagtac cacgtttacc
    10141 gtccaacata acacggttaa tcaagcgagt taccaatttt gagttataca atggatctgg
    10201 caatacttcg cgcttaggcg cttgattttt acgactcatt tatatttccc ccttcttagc
    10261 cttttggacg tttagtaccg tatttagaac ggccttgctt acgatcgttt acaccagcag
    10321 tatcaagtgc accacgaacg atatggtaac gtacccctgg aaggtctttt acacgtccac
    10381 cacgaagaag aaccacactg tgttcttgca agttgtgacc gatacctggg atgtaagcag
    10441 taacttcgat aaggttgctc aaacgtacac gagcaaattt acgaagggct gagttaggtt
    10501 ttttaggtgt cattgttccg acacgagttg caacaccgcg tttttgtggt gatgaaacgt
    10561 ttgtttgaac ttttttacgg ctgttgtaac caacgttcaa agctggtgat ttagattttt
    10621 ctactttaga cttacgtggt ttacgtacca actggttaat tgtaggcatc tacattctcc
    10681 tgtatatatt ttttattttt ggtgataggg cattggtgac aacccctatc tgtgtgtact
    10741 tttgcaacat ttgtcagcac gtccctgtac actcttgaga gaccaaaagt aaaaagtacc
    10801 gtctaatata gtaccacatc ccaaaaccat tgtcaaatag atatgtttga aattttcaag
    10861 aaaaaaacac agccaagtag ctgtgtcatt ttgctatttc ttagtagccc atacccattc
    10921 catccatacc ttgtggcata gctggagcag ctggttctgg cttgttggca acaactgctt
    10981 ctgtcgtcaa aatcaagctg gctacagaag ctgcgttttg aagggccgaa cgagtcacct
    11041 taacagggtc aataatacct gcatccatca tattgaccca ttcgcctgtc gcagcgttaa
    11101 agccagtgcc aagctcagaa ttcttcaatt tatcaatgat aactgaacct tcatagcctg
    11161 cgttataggc aatctgacga actggttctt ccaaggcacg aaggacaata ttgcgtcctg
    11221 tttcatcgtc gcctttcaac tccaatttcg ctacgctatc gataacattt acaagtgctg
    11281 taccaccacc ggcaacgatt ccttcttcaa ctgcggcacg tgttgcgttg agggcatctt
    11341 cgatacggag tttcatttct ttcaactcag tttccgtcgc ggcaccgact ttgataactg
    11401 cgacaccacc tgacaattta gccaagcgct cttgcaattt ttcacggtca aattctgaag
    11461 tggttacctc aatttgtgac ttgataacag ccacacggtt ggcaatggct tcaggattgc
    11521 cagcaccttc aacaatggtt gcaccatctt tgtcgaccac aaccttggca gcttgaccaa
    11581 gggcttcaat ggtcgcatct ttcaagtcca aaccaaggtc ttctgtaatg actgtaccac
    11641 ctgtcaagat agcgatatct tccaacatgg ctttacgacg gtcaccaaag cctggagctt
    11701 tgactgcaac aacgttgaat gtaccacgaa tcttgttgag aacaagtgta ggaagagctt
    11761 cgccatccac atcgtcagca ataatcaaaa gcggacggtt ggcctgaaga atgctttcca
    11821 agagtggcaa aatatcttgg atatgagaaa tcttcttatc tgtaatcaag ataaatggat
    11881 tttcaagttc agctaccatc ttttcattat ctgttaccat gtattgtgac agataaccac
    11941 ggtcgaattg cataccttca accacatcca attctgtttc cataccgcga gattcttcga
    12001 tagtaataac gccgtctgtt cccacgcgct ccatagcttc tgaaatgtac tcaccaactt
    12061 tttcagaacg tgaagacacg gctgctacct gagcaatttc agctttattg gatacaggac
    12121 ttgcttgtgc tttcaaggct tcaaccgcag tcgcaaccgc ggcttcaatc ccgcgacgaa
    12181 ttccgattgg gttggcacct gcagttacgt ttttcaagcc ttcacgtacg atagcttggg
    12241 tcaagacagt agcagttgtt gtcccgtcac cggcaatgtc attggttttt gaagccactt
    12301 cggataccaa cttagcaccc atattttcaa aatgatcttc caactcgatt tctttagcaa
    12361 tggttacacc gtcgtttgta atgagtggtg aaccgtaagc tttttccaag acaacattac
    12421 gaccttttgg acccaaggtt actttgactg tgtcagccaa aatatcgaca ccacggacca
    12481 tgctttcacg cgcatctgct gcaaatttga tttcttttgc catttttcta cctctattct
    12541 acaattgcca aaatatctgt ttcacggatg atgtgaacgt tctgatcacc gtctttgact
    12601 tccaatcctg cgtgagcatc aatcaaaaca gtatctccaa ctgccaccgc tggggcaacc
    12661 aagtctccgt tcaaggtccg aatcccttgt ccgatcgcta ggacacttgc ttcttttgtc
    12721 ttttcttggc ttgcgcctgc taggacaaag ccaccaactg tttgttcttt ttcttcaatt
    12781 tttacggcga tacgatcgcc caatggtttc aacatggtat tacctcctgg tctttttagc
    12841 actctttgta ttcgagtgct aattcacatt tcctattgta tcacttggtc aaaaatagtc
    12901 aagaacattc tatccctttt tcatgaaaat ccatcttgag accgagacag aacttgccta
    12961 tccattaccg ctaaattatg ctatactatt tttaaaaagt tagggggtat tcatgaaatt
    13021 ttttagagat ttaaaaattg attaccttga aagccgattt tcagcctacg aaagttttgc
    13081 agaatggttt ttaaaacg
//
LOCUS       DBNFCK010000050        12928 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.050, whole genome shotgun sequence.
ACCESSION   DBNFCK010000050 DBNFCK010000000
VERSION     DBNFCK010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12928
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.050"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..530
                     /locus_tag="AC1PIW_001114"
     CDS             <1..530
                     /locus_tag="AC1PIW_001114"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606057.1"
                     /translation="AMKTNLLKTAGIVAACVRYFFYLLIASYIFFSLAGLGGDSFAIG
                     SWEYTFNKPIDQISVWLILLATGLILVVLVALSHIAHLIQKLCKLLLEEDYFASASME
                     LYQKLFISLLVLTAGQFCLTSLITLTNAAGPNNFLNLRWSDFILNALFIFLTYFVWTL
                     VQKGQQLETENSEFI"
     gene            532..741
                     /locus_tag="AC1PIW_001115"
     CDS             532..741
                     /locus_tag="AC1PIW_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIP6606058.1"
                     /translation="MEMIRVNLDKVLKDRQMTSKDLAEQVGITEANLSILKTGKAKGI
                     RFNTLMSICRILDCQPGDILEYVEE"
     gene            complement(865..1536)
                     /locus_tag="AC1PIW_001116"
     CDS             complement(865..1536)
                     /locus_tag="AC1PIW_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIP6606059.1"
                     /translation="MDINWQAVFNADIAREAIPQILEGLPYTLSLSLIGFALGTFCGF
                     FVALMRMSKFGPLRWLAMAHISLMRGIPLMVLLFFIYFGLPFMGLQLDAISASIIAFT
                     SMSSAYISEIIRASLSAIDKGQWEAARSLGLRTNVIHRKIIIPQAFRIALPPLSNVLL
                     DMVKSTSLTAMITVPEIFNKAKIVGGAKSDYMTVYICVALIYWVICTLYAFGQLKLEK
                     RLATY"
     gene            complement(1627..2502)
                     /locus_tag="AC1PIW_001117"
     CDS             complement(1627..2502)
                     /locus_tag="AC1PIW_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIP6606060.1"
                     /translation="MLKKVLASALLVSTLTLAACSSSSSNTSSSSAEQTQWEKIQEAG
                     VLKVATPGTLFPTSYYNDAKELVGYEIDMINEIGKRLKLEIEYQEIGVAEAFTAVDSG
                     KVDISVNNFDMTEARKEKYNFSIPYKYSVGGYIVREDGSSGIEAADLSDWTGKKAGGG
                     AGTQYMKIAEKQGAEPVIYDNVTNDVYLRDVSTGRTDFIPNDYYTQVMAIKWANANFP
                     DIKVKMGEAKYNPTEQGIVMSKADTSLKEKIDEALEAMKADGTLKAISEKYYAGQDLT
                     VPVENADKLPVIEVE"
     gene            complement(2627..4075)
                     /locus_tag="AC1PIW_001118"
     CDS             complement(2627..4075)
                     /locus_tag="AC1PIW_001118"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIP6606061.1"
                     /translation="MIWKDATAMAQAVNQKQVFAKELVQETIDRIEKLNPTLNAVVSK
                     QYEEALKEAEKEDYLGKPFAGVPFLLKDLGQNEKGQPSSAGSRLLAGRPAGHTDTYVQ
                     RLKDLGFIIVGRTNTPEFGFKNISDASLHGPVNLPLDPSRNAGGSSGGAAAALASGMV
                     SIAAASDGGGSIRIPASFNGLIGLKTSRGRIPVGPKSYRGWQGASVNFALTKSVRDTQ
                     RLLYHFQDYQLEAPFPLAKLSEEEVFGKLSRPLKIAYYTKSPVGSKVSLEAVEAVQKA
                     CHHLANLGHEVVELTEYPLDGVALMKSFYLMNSVDTAQMFDEIEAAFGRQMTLDDMEV
                     MSWAIYQSGQTIPAKLYSKALLDWDQFGASMARFHEEYDLLLTPTVADVAPKHGQFDL
                     PVDLLDRLKQTQNYSMEEQQDLIWQMFEDSLALTPFTQQANICGQPAISLPTYVRADS
                     LPIGVQLTASKGREDLLLQLADQMEAAGLLNL"
     gene            complement(4085..4912)
                     /locus_tag="AC1PIW_001119"
     CDS             complement(4085..4912)
                     /locus_tag="AC1PIW_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HIP6606062.1"
                     /translation="MKSWVENVTIKKYEDRSGLTGLKQEPLVWTEVFLREVNRQSNTG
                     ILHFWPMDQTVILGMMDSQVAQLDKGLASIGQAGYSPIIRSLGGLAVVADEGILNVTL
                     ILSNPSGHKVDLRESYQVMVDLIAQALSDFPFEVVSGEVATSYCPGTYDLSIKGRKFA
                     GLAQRIYQEAIAISAYISVSGNQVKRGQVVADFYVASFAPQEVSDRFPQVNPDSMANL
                     SDLVGQDVTVEDMKKRIEQVLVENGTRLSTFYPSSDNMADFMTFGKTIKQSMEKYGI"
     gene            complement(5188..6324)
                     /gene="dnaJ"
                     /locus_tag="AC1PIW_001120"
     CDS             complement(5188..6324)
                     /gene="dnaJ"
                     /locus_tag="AC1PIW_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIP6606063.1"
                     /translation="MNNTEFYDRLGVSKNASPDEIKKAYRKLSKKYHPDINKDPGAED
                     KYKEVQEAYETLSDPQKRSAYDQYGPAGANGGFGGGAGGFGGFDGAGFGGFEDIFSSF
                     FGGGGATRNPNAPRQGDDLQYAVNLKFEEAIFGVEREVSYNREATCRTCTGSGAKPGT
                     SPVTCGRCHGSGVINVDTQTPLGTMRRQMTCDVCHGRGKQIKDPCTTCHGTGHEKQAH
                     TVTVKVPAGVETGQRIRLAGQGEAGFNGGPYGDLYVVIQVQPSDKFEREGTTIHYKLN
                     LNFVQAALGDTVHVPTVHGDVDMVIPEGTQTGKTFRLKGKGAPSVRGGAIGDQYVTVN
                     IVTPTGLNDRQKAALKEFAAAGNIDVKPHKKGFFDKVKDAFEDL"
     gene            complement(6756..8579)
                     /gene="dnaK"
                     /locus_tag="AC1PIW_001121"
     CDS             complement(6756..8579)
                     /gene="dnaK"
                     /locus_tag="AC1PIW_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIP6606064.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTIISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     EYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     DEKILVFDLGGGTFDVSILELGDGVFDVLATAGDNKLGGDDFDQKIIDHMVAEFKKEN
                     GIDLSADKMALQRLKDAAEKAKKDLSGVTSTQISLPFITAGAAGPLHLEMTLTRAKFD
                     ELTYDLVERTKIPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRND
                     VDQAIFATEKTLKETEGKGFDAERDQAQAALDELKAAQEANNLDDMKAKLENLNEKAQ
                     ALAVKLYEQAAAAQQAAAGQEGAQTANNADDDVVDGEFTEK"
     gene            complement(8676..9188)
                     /gene="grpE"
                     /locus_tag="AC1PIW_001122"
     CDS             complement(8676..9188)
                     /gene="grpE"
                     /locus_tag="AC1PIW_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIP6606065.1"
                     /translation="MSEEIKNEEIVEEVEATEEVVETPEKSELDLANERAEEFENKYL
                     RAHAEMQNIQRRANEERQTIQRYRSQDLAKKILPSLDNLERALQVEGLTEDVKKGLEM
                     VQESLIQALKEEGVEEVATDVFDPNLHMAIQTVPATDDCPAEHIAQVFQKGYKLHERL
                     LRPAMVVVSE"
     gene            complement(9201..10235)
                     /gene="hrcA"
                     /locus_tag="AC1PIW_001123"
     CDS             complement(9201..10235)
                     /gene="hrcA"
                     /locus_tag="AC1PIW_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIP6606066.1"
                     /translation="MITQRQNDILNLIVELFTRHHEPVGSKALQEMIASSSATIRNDM
                     AKLEQLGLLEKAHTSSGRMPSRAGFQYFVNHSLNLKHINEEDVYQVVKAFDFEAFKLE
                     DILERASQVLADLTGYTSVILDVEPTSQQLTSFDIVQLSSHDALAVLTLDQSKPVTVQ
                     FAIPKNFLTRDLEVLKRLVDERFVGQTVLAIHYKLRTEIPQVVQRYFATTDNVLDLMD
                     YIFSNLFQESVFISGKVASLTYGNLATYQLLDSPQLLAPELRQGLAPNQQTSISVAEH
                     REPALADVTVIHHRFPIPYRGMGQMSLLGPVDMDYRRQMSLINIISRILFMKLTDYYR
                     YLSSNHYEVN"
     gene            complement(10554..11249)
                     /locus_tag="AC1PIW_001124"
     CDS             complement(10554..11249)
                     /locus_tag="AC1PIW_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIP6606067.1"
                     /translation="MADVRLYISRHGKTMFNTIGRVQGWCDTPLTKVGEEGIRELGLG
                     LKDAGLDFKLAVSSDLGRTVQTMTIAQRELGILGKIPYYQDKRIREWCFGSFEGMYDA
                     ELFQGVLPRLKGTVDATGMSFAEIAAGIQEADTAGWAESWEVLSNRILTGFESIAQDL
                     EKQGGGNALVVSHGMTIATLAHLLEPERGANVFLDNGSITVLKYENGKLLIEAVGDLS
                     YRKRGAELIAQGK"
     gene            complement(11325..12662)
                     /locus_tag="AC1PIW_001125"
     CDS             complement(11325..12662)
                     /locus_tag="AC1PIW_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HIP6606068.1"
                     /translation="MDIRQVRETIEMIEEQNFDIRTITMGISLLDCIDSDIDKAAEKV
                     YTKIVTKAKNLVAVGDEIAAELGIPIVNKRVSVTPIALIGAATDATDYLPLAHALDKA
                     AHEIGIDFIGGFSALAQKGYQKGDEILINSIPQALAQTSKVCSSVNIGSTKTGINMTA
                     VRDMGRIIKETAEASDMGAAKLVVFANAVEDNPFMAGAFHGVGEADVVINVGVSGPGV
                     VKRALEKVRGESFDVVAETVKKTAFKITRIGQLVGNMASERLGVKFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDAVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPETTPAETIAAMIADEAAIGVINQKT
                     TAVRIIPLGKEGDMIEFGGLLGTAPVMKVNQASSVDFINRGGQIPAPIHSFKN"
     gene            complement(12674..>12928)
                     /locus_tag="AC1PIW_001126"
     CDS             complement(12674..>12928)
                     /locus_tag="AC1PIW_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIP6606069.1"
                     /translation="VTVVGKDKSGIVAGVATKIAELGLNIDDISQTVLDEYFTMMAVV
                     SSDEKKDFTKLRAELEAYGQALNVKINIQSAAIFDAMHNL"
BASE COUNT         3419 a         3153 c         2737 g         3619 t
ORIGIN      
        1 ttgctatgaa aaccaatcta ctcaaaacag ctggcattgt ggctgcttgt gttcgctatt
       61 tcttctattt attaattgca agctatattt tctttagcct ggctggtctt ggaggcgact
      121 cctttgcgat aggctcttgg gaatacacct ttaacaaacc cattgaccag atcagtgtct
      181 ggctcattct gctagcgact ggattgattc tagttgtctt agtagcactc agtcacattg
      241 cccacttgat tcaaaagtta tgtaaattgc ttctggaaga agattatttc gcttcagcta
      301 gtatggaact ttatcagaag ctcttcatca gtttgctcgt cctgactgct gggcaatttt
      361 gcctaaccag cttgatcacc ttgacaaatg ctgctggacc aaacaacttc ctcaatctgc
      421 gttggtcaga tttcatcctc aacgccctct ttatcttctt gacttatttt gtctggacct
      481 tagtacaaaa ggggcaacaa cttgaaaccg agaacagcga gtttatctga tatggaaatg
      541 attcgcgtaa acttagacaa ggtcctcaaa gaccgacaaa tgacttccaa ggacttggcg
      601 gagcaagtcg gcatcaccga agccaatctg tccattctca agacaggcaa ggcaaaaggc
      661 attcgtttta ataccctgat gagcatctgc cgcatcttag actgccaacc aggggatatt
      721 ttggaatatg tggaagagtg agagttacat cgaaaaaaca gtctttaaaa taaaaaatag
      781 cttaggtcca catcattcga gacgttaatt ctaagctatt tttgattgtt caaattttaa
      841 tcggaaggct aaaccaacct ctttttagta agtcgccaag cgtttttcta gcttgagctg
      901 accaaatgca taaagggtac agataaccca ataaatgagg gctacacaga tataaaccgt
      961 catataatcc gacttagctc caccgactat ttttgcctta ttgaaaatct ctggtacagt
     1021 aatcatagcg gtcaaggacg tacttttcac catatccaag agaacattgc tcagtggtgg
     1081 aagagcaatc cgaaaggctt ggggaataat gatttttctg tgaatcacat tggtccgcaa
     1141 gcccagggaa cgcgctgcct cccattgtcc cttatcaatg gccgaaagag atgcacgaat
     1201 aatttcagaa atgtaggcac tggacatgga ggtaaaggcg ataatagatg cggagatagc
     1261 atctagttgt agccccatga aaggcaatcc aaaatagatg aaaaagagaa gaaccatgag
     1321 gggaattcca cgcatgagag aaatatgggc catagccaac caacgcaagg gtccaaactt
     1381 agacatccgc atgagggcta caaaaaatcc acagaaagtc cccaaggcaa aaccaattag
     1441 ggatagggat agagtgtagg gaagtccctc caaaatctgt ggaatagcct ctcttgcaat
     1501 atctgcatta aaaactgcct gccagttaat atccatatct tctcctcttt cctaaaaacc
     1561 agatagacct tgccagtctg tgactgacaa agtctataca agatgcattt aatttgttac
     1621 gaggtcttat tcaacttcga taactggcaa tttatcagca ttttcaacag ggactgtcaa
     1681 gtcttgtcct gcatagtatt tctcagaaat tgccttcaaa gttccatcag ctttcatcgc
     1741 ttcaagtgct tcgtcaattt tttctttcaa gcttgtgtca gccttgctca taacaatacc
     1801 ttgttcagtt gggttgtatt tagcctcacc catttttacc ttgatgtctg ggaagttggc
     1861 atttgcccat ttgatagcca tcacttgtgt atagtaatca tttggaatga agtctgtacg
     1921 acctgtagat acatcacgca aatagacatc gttggttaca ttgtcataga taactggctc
     1981 tgcaccttgc ttttcagcga ttttcatgta ctgagtacca gcgccaccac cagctttctt
     2041 gccagtccag tcgctcaagt cagctgcttc gattcctgat gaaccatctt cacgaacaat
     2101 gtagccacca actgaatact tgtaagggat agagaagttg tacttttcct tacgtgcttc
     2161 tgtcatgtca aagttgttaa cagaaatatc taccttacca ctgtcaaccg ctgtaaaggc
     2221 ttctgccaca ccgatttctt ggtattcaat ttccagtttc aaacgcttac caatttcatt
     2281 gatcatgtca atttcatatc cgaccaattc tttagcatca ttgtagtaag aagttgggaa
     2341 gagggtacct ggtgtagcaa ctttcaagac accagcttct tgaatttttt cccattgagt
     2401 ctgttcagca gaagaactgc tagtattcga acttgaacta gaacatgctg ccaaggtcaa
     2461 tgtagaaacc aagagagctg aggctaaaac ttttttcaac ataaaacaat ctcctttgga
     2521 agccatagct tcctttttta ctttttcggt gttaacccac caacttttac aggttaacat
     2581 tttgaaaacg ttttggcaaa taattgaact gaaaaaatga attcccttac aagttgagta
     2641 aaccagctgc ctccatttga tctgccaact gcaagagcaa atcctctcgt cccttggatg
     2701 ctgtcaattg aacgccgatt ggcaggctgt cagcacgtac ataagttggc agagaaatag
     2761 ctggctgacc acaaatattg gcctgctgcg taaagggagt taaggccaga ctgtcctcga
     2821 acatttgcca gatcaggtcc tgctgctctt ccatggaata gttctgggtc tgcttgaggc
     2881 gatccagcaa atctactggt aggtcaaatt gtccatgctt gggcgctaca tcagccactg
     2941 tcggcgtcag gagcaagtcg tattcctcat ggaaacgcgc catgctggct ccaaactggt
     3001 cccagtccaa tagggccttg gagtacaatt tggctggaat agtttgcccg ctttggtaaa
     3061 tagcccagga catgacttcc atgtcatcca aggtcatctg gcgcccaaaa gctgcttcga
     3121 tttcgtcaaa catctgggcc gtgtccacgc tgttcatcag gtagaaggat ttcatcaggg
     3181 ccactccatc cagaggatat tctgttaatt ccaccacttc atgccccaaa tttgccaagt
     3241 gatgacaggc tttttgaacc gcttctaccg cttcaagact gaccttgctc cctactggag
     3301 atttggtata gtaggcgatt tttagggggc gagacaattt tccaaaaacc tcctcttcac
     3361 tcagcttggc aaggggaaaa ggagcttcca gctggtagtc ctgaaaatga tagagcagac
     3421 gctgggtgtc acggacagac ttggtcaggg caaaattgac agatgctccc tgccagccac
     3481 gataagactt aggtccaact ggaatccgac cacgactggt tttgagcccg atcaagccat
     3541 tgaaggaggc tgggatgcga atagacccac caccatccga tgctgcagca atagagacca
     3601 tgcctgaagc cagtgctgca gctgcaccac cacttgatcc accagcattg cgactggggt
     3661 ccaagggaag attgacagga ccatgcaggc tggcgtctga gatatttttg aagccgaatt
     3721 ctggcgtatt ggttcgaccg actatgatga aacccaaatc tttcaagcgt tggacatagg
     3781 tgtcggtatg gcctgctggt cgaccagcaa gtaggcgaga accagcagaa gatggctgcc
     3841 ccttttcatt ttgccccaga tccttcaaca agaaaggaac tccagcaaat ggcttgccca
     3901 gatagtcttc tttctccgct tctttcaagg cttcttcata ctgcttgctg acaaccgcat
     3961 tgagggtggg attgagcttt tcaatcctgt caatcgtttc ttgcaccaat tccttggcaa
     4021 aaacctgttt ttgattgaca gcctgagcca tggctgtcgc atctttccat atcataagaa
     4081 tctcctagat accatatttt tccatagact gcttgatggt ctttccaaag gtcataaaat
     4141 cagccatgtt gtccgacgag ggataaaaag tagacagcct ggtcccattt tcaaccaata
     4201 cttgctcaat ccgtttcttc atatcctcca ccgtcacatc ctgaccgacc aagtctgaca
     4261 gattggccat cgaatccgga tttacttgcg gaaatctgtc cgatacttct tgcggagcaa
     4321 aacttgccac ataaaaatcc gccaccactt gcccacgttt gacttgatta cctgacacac
     4381 tgatataggc agaaatggca atagcctctt ggtaaatccg ctgggccaat ccagcaaact
     4441 tccgcccctt gatactaagg tcgtaggtcc ctggacagta ggaagttgcc acctcaccac
     4501 tgacaacctc aaagggaaaa tctgaaagtg cctgggcaat caaatccacc atgacctgat
     4561 aagactcccg caaatcaacc ttgtgaccag aaggattgga caaaatcaga gtgacattta
     4621 aaatcccttc atctgcaacg actgccaaac cgcccaaact ccgaataata ggactgtagc
     4681 ctgcctgacc aatactggct aagcccttgt ccagctgggc aacctgacta tccatcatcc
     4741 ccagaatgac cgtttggtcc atgggccaaa aatgtaaaat tcctgtattg gactgacgat
     4801 tgacctcgcg caaaaagacc tcagtccaca ccagtggctc ctgtttcaaa cccgtcaagc
     4861 cagaccgatc ttcgtatttc tttatggtca cattctcaac ccaagatttc attcgtaagc
     4921 gttctctttc aaattactat atcttatttt aacacagagc ctgtgatgcg tcggagaaaa
     4981 tgcccagagt tttttctgaa aaagaaaggg gagtgggaaa gaactcgacc aagataaaaa
     5041 gagttcgtct tcccaccccc gcacagttga ttaggccaga tttggagtgc aaaacacgaa
     5101 caaatctgcc aatcaaccac tgcgctgaga tgttgaaacg aactctgagt agtggtcctg
     5161 ggctttttgc ccagtctcat cttagaatta taaatcctca aaggcatctt ttaccttgtc
     5221 aaaaaatcct tttttatggg gtttgacatc aatatttcca gctgccgcaa attctttaag
     5281 agctgctttt tggcggtcat tcaaacctgt cggagtgacg atatttactg tgacatattg
     5341 atcaccaata gctccaccac gaacacttgg tgcacctttt cctttgagtc ggaaggtctt
     5401 accagtctgg gtaccttcag gaatgaccat atccacatca ccgtgaactg ttggcacatg
     5461 aacagtatct ccaagtgctg cttggacaaa gttgaggttc agtttatagt gaatggtggt
     5521 cccttcacgt tcaaacttgt ctgaaggttg gacctgaatg acaacgtaga gatctccgta
     5581 tggacctcca ttgaaaccag cttcaccttg accagccaag cgaatacgtt gaccagtttc
     5641 cacaccagct ggcaccttaa ccgttaccgt atgggcttgt ttttcatgac cagttccatg
     5701 acaggttgta catgggtctt tgatttgttt tccgcgacca tgacagacat cacaggtcat
     5761 ttgacgacgc atggttccaa gtggagtttg ggtgtccaca ttgatcactc cagaaccatg
     5821 acaacgccca caggttaccg gacttgttcc aggtttggca cctgaacctg tacaggttcg
     5881 acaagttgct tcacgattgt aggaaacttc tcgttccaca ccaaaaatcg cttcttcaaa
     5941 tttcaaattg accgcatatt gcaaatcatc cccttgacga ggtgcattag gattgcgcgt
     6001 cgcaccacct ccaccaaaga aactagagaa aatgtcttca aagccaccga aacctgctcc
     6061 gtcgaaacca ccaaagccac ctgctcctcc gccgaagcct ccattagcac cagcaggacc
     6121 atactggtca taggcagacc gtttttgcgg gtcgctcaag gtctcataag cctcctggac
     6181 ctccttgtat ttatcctccg cacccggatc cttgttaata tctggatggt atttctttga
     6241 aagcttccga taagccttct taatctcgtc tggcgaagca ttcttagaaa caccaagacg
     6301 atcgtaaaat tcagtattgt tcatataaga taccaagccc gttaaaagca aagtgaaaat
     6361 aggaaatcgg cagaaaatcc tgattttcta gacgatttat ctttttcaca cagcttttag
     6421 ggcgagttca tttcctttcg ttaaataata gacgaaactc agaaagtagc ccgtgttcag
     6481 ttacgaacac tttcctttct gtaaaagtta gacgggtatt tacccaaaaa cagaggctgg
     6541 gttgcaacct ctgtgattgg aatctttatt aactaataat ttctattctc gggaagagga
     6601 gaactgagtt caagctaaga actacggaaa aaagataact ttccttgttc tagatactca
     6661 aagaaattca agactaggct aggcaactgg acgaagattg aactggagtt catcgaggag
     6721 agttaacgaa gcctagtgaa gaatttcgaa gagtactact tttcagtaaa ctcgccatct
     6781 accacatcat catctgcatt gttagctgtt tgggcacctt cttggcctgc ggctgcttgt
     6841 tgtgctgctg cggcttgctc gtagagtttc actgcaaggg cttgggcttt ttcgttgagg
     6901 ttttcaagtt tagccttcat gtcgtccaag ttgttagctt cctgagctgc tttcaactca
     6961 tcaagagcag cttgggcttg gtcacgttct gcgtcgaagc ctttgccttc tgtttcctta
     7021 agagttttct cagtcgcaaa gattgcttgg tcaacatcgt tacgaaggtc cacttcttcc
     7081 ttacgtttct tatctgcttc cgcgttagct tctgcatctt tcatcatgcg gtcgatttct
     7141 tcatctgtca aacctgagtt agactggata acgatagttt gttctttttg tgtaccaagg
     7201 tctttggctt ttacagaaac gataccgttc ttgtcgatgt cgaatgttac ttcgatttgt
     7261 ggaataccac gaggtgctgc agggatgtct gtcaattgga aacgaccaag agtcttgttg
     7321 tccgctgcca ttggacgctc accttgaagc acgtggatgt caacagctgg ctggttgtcc
     7381 gctgcagttg agaaaacttg tgatttagaa gttgggattg ttgtgttacg gtcgatgagt
     7441 tttgtaaata caccacccat tgtttcgata ccaagtgaca atggtgttac gtcaagaaga
     7501 acaacatctt tgacatcacc agtaatcaca ccaccttgga ttgccgcacc catggcaacc
     7561 acttcgtcag ggtttacaga tttgtttggc tctttaccag tttcagcttt tacagcttcc
     7621 acaacggctg ggatacgagt tgaaccaccg acaaggatta cttcatcgat ttctgatagg
     7681 ctgagacctg catctgaaag ggcttgacga acaggaattt ttgtacgttc tacaaggtcg
     7741 taagtcaatt cgtcaaattt cgcacgagtc aatgtcattt ccaagtgaag tggacctgct
     7801 gcacctgcag tgatgaatgg caagctgatt tgagttgaag tcacgcctga caagtctttc
     7861 ttagcttttt cagctgcatc tttcaaacgt tgaagggcca ttttgtcagc tgacaagtcg
     7921 ataccgtttt ctttcttgaa ctctgctacc atgtggtcga taatcttttg gtcaaagtcg
     7981 tcaccaccga gcttgttatc acctgctgtt gcaagtacgt caaagacacc gtcaccaagt
     8041 tcaaggatag atacgtcgaa tgtaccgcca ccaaggtcga ataccaagat tttttcatct
     8101 ttgtcagtct tgtccaaacc gtaagcaaga gctgctgcag ttggttcatt gacgatacgt
     8161 tctacttcaa gaccagcgat tttaccagcg tctttggttg cttgacgttg ggcatcgttg
     8221 aagtaagcag gaacagtgat aaccgctttg gttacttttt caccaagata ttcttcagcg
     8281 taacctttca agtattgaag aatcattgct gagatttctt gtggtgtgta ttctttaccg
     8341 tttgctgaaa ctttttcaga agttcccatt tttgatttga tagagatgat ggtatctggg
     8401 ttggttaccg cttggcgttt tgccgcgtca ccaacgatga tttcaccatt tttgaaagac
     8461 acaacagacg gagttgtgcg gtttccttct gggtttgcaa tgatttttga ttcagttcct
     8521 tcaagaactg caactgctga gtttgttgta cctaagtcaa taccgataat tttagacata
     8581 tgtgttacct ctttttaatt tactttttat gttcaattta gcctagttta atgacatttc
     8641 ggtcaggttt tcttttttgg gaaatagcag agcgactatt ccgacaccac taccatagcc
     8701 ggtctcagca agcgttcatg cagcttgtag cctttttgga agacttgtgc gatgtgttct
     8761 gctgggcaat cgtctgtggc tgggactgtt tgaatggcca tgtgaagatt tgggtcaaag
     8821 acatctgttg cgacttcttc caccccttct tctttaaggg cttgaatcaa gctttcttga
     8881 accatttcca agcctttttt gacatcttct gtcaagcctt cgacttgaag ggcacgttcc
     8941 aagttatcca agctcggcaa gattttcttg gccaagtctt gcgaacggta acgctgaatg
     9001 gtttggcgtt cttcgtttgc acggcgttgg atattttgca tttcagcgtg ggcacgaagg
     9061 tacttgtttt caaattcctc agcgcgctcg tttgccaaat ccaattctga tttttcaggt
     9121 gtctccacaa cttcttctgt tgcttcaacc tcttctacga tttcttcgtt tttgatttct
     9181 tctgacaaag cattctcctt ctaattgact tcgtaatgat tactacttag ataacggtag
     9241 taatcggtta atttcataaa taggatgcgg ctgatgatgt tgattaagct catctgccta
     9301 cggtagtcca tgtcaacagg accgagcaaa ctcatctggc ccatgccccg ataggggatt
     9361 ggaaaacgat gatgaatgac cgtcacatct gcaagagcag gttctctatg ttccgccacg
     9421 gaaatgctgg tctgctggtt gggagccaag ccctgccgca actctggtgc caataactga
     9481 ggactatcca agagttggta ggtggcaaga ttgccgtagg tcagcgaggc aacttttcca
     9541 ctgataaaga ccgattcttg gaaaagattg gaaaagatgt agtccatgag atccaagaca
     9601 ttgtccgtcg tggcaaagta acgctgcacc acttggggaa tctccgtccg caacttgtag
     9661 tggattgcca agaccgtctg cccaacgaag cgctcatcaa ctaagcgttt gagcacttcc
     9721 aaatccctag tcaagaagtt cttaggaata gcaaactgaa cagtgacagg tttggactgg
     9781 tccagagtca agactgccag agcatcgtgg ctactgagct gcacaatgtc aaaggaagtc
     9841 agctgctgac tggtcggctc cacatctaaa atgaccgagg tgtagcctgt caaatccgct
     9901 aggacctgac ttgctcgctc caagatgtcc tctagcttga aggcttcaaa gtcaaaggcc
     9961 ttaaccactt gataaacatc ttcttcattg atgtgtttca gattgagcga gtggttgaca
    10021 aagtattgaa aaccagcccg actgggcata cgaccacttg atgtgtgggc cttttccagc
    10081 aaacccaact gctctagctt ggccatgtca ttacgaatgg tagccgaact tgaagcaatc
    10141 atttcctgca aggccttaga accaactggc tcatgatggc gcgtaaataa ttcaacaatc
    10201 agattcaaaa tatcattttg acgttgggta atcatctccg ctcctttcat gaacactgtt
    10261 ttagttacgc tcagcgaaaa tcgacagtag cctaggaagc gaggccgaat gtagaactat
    10321 ggttcacaaa ggcaagctga caacggatag tgttgatttt tgaagagcgt tagcactcta
    10381 tgatatcgag tgctaattac ttatactccc attatacgcc gaaaaaatgg attgtcaaga
    10441 gaaaaagaca aaaaattagc actcttttgc ttagagtgct aaaaatcagt ctgaggacct
    10501 agaatggtgg atgtcaagaa aaaaaatcag agaatcctcc ctgattttat atcttatttc
    10561 ccttgggcaa tcaactctgc cccacgtttg cggtaggata aatccccaac tgcttcaatt
    10621 aaaagcttgc cattttcgta tttgaggacg gtgattgagc cgttatcgag aaagacattg
    10681 gcaccacgct ctggctctaa caaatgagcc aatgtcgcaa tagtcatacc gtggctgacc
    10741 acaagggcat tgcctccgcc ttgtttttcc aagtcctggg cgatggattc aaaacctgtc
    10801 aagatacggt tgctcaaaac ctcccacgat tccgcccagc cagcggtgtc tgcttcttgg
    10861 atacctgctg cgatttccgc aaaggacata cccgtcgcat caactgtccc tttcaaacga
    10921 ggcaggaccc cttggaaaag ctcggcatcg tacatgcctt caaaactacc gaaacaccat
    10981 tcacggatac gcttgtcttg gtaataaggg attttcccta aaattcctaa ctcacgctgg
    11041 gcgatggtca tggtctgaac ggttcgaccc agatcactgg atacggctag tttaaagtca
    11101 aggcccgcat ccttgagccc caagcccagt tcacgaattc cttcctcacc gaccttggtc
    11161 agcggcgtat cacaccagcc ctgcacgcgc ccaatggtat tgaacatggt tttcccgtga
    11221 cgagaaatat ataatcttac atctgccatt cgtttttctc cttgtcattt cttactatta
    11281 gtataacaaa aaaccgactg cttgtggcaa tcggtctacg ttttttagtt cttaaagcta
    11341 tgaatcggtg ctggaatctg accaccacgg ttgataaagt ccacagacga agcttgattg
    11401 accttcataa caggagctgt tcccagaagg ccgccaaatt caatcatgtc tccttctttt
    11461 ccaagcggaa taatacgaac cgcagttgtt ttctgattga taaccccaat agccgcttca
    11521 tccgcaatca tagccgcaat ggtttcagca ggcgttgttt ctggaatggc aatcatgtcc
    11581 aaacctacag aacagatagc cgtcatggct tccaattttt caaggttgag cgagccattt
    11641 tgcaccgccg caatcatgcc ctcatcctca gacacaggga taaaggcacc tgacaagcca
    11701 ccgacttggt tgcaggccat gaccccaccc tttttgactg cgtcattgag caaagccagg
    11761 gcagcagtcg ttccgtgcgt accaacggtt tctaagccca tttcctccaa aacccgtgct
    11821 actgagtcgc caacagctgg tgttggagca agcgacaggt ccacgatacc gaacttaacc
    11881 cccaggcgtt cactggccat attgccgacc aactgaccga tacgggtaat cttgaaagcc
    11941 gtcttcttga cggtctccgc caccacatca aagctctcac cacgcacttt ttcaagggca
    12001 cgcttgacca caccaggccc agacacgccg acattgatga ccacatccgc ctcgccgaca
    12061 ccgtggaagg cccccgccat gaaaggattg tcctcaaccg cattggcaaa gaccaccaac
    12121 ttggcagccc ccatatcaga agcctccgcc gtctccttaa tgatccgccc catgtcccgc
    12181 acagccgtca tattgatacc cgtcttggtc gagccgatat tgactgacga gcagacctta
    12241 gaggtttggg ccaatgcctg cggaatagag ttgatgagga tttcatcacc tttttggtag
    12301 cccttttgag caagagctga aaaaccaccg ataaaatcga tgccgatctc atgagccgcc
    12361 ttgtccaagg catgagctag gggaaggtaa tccgtggcat ccgtcgccgc accaatcaaa
    12421 gcaataggcg ttactgacac ccgtttgttg acaatgggaa tgcctagttc tgccgcaatc
    12481 tcatcgccga ctgctaccaa gttcttggcc ttggtgacaa tcttggtata cactttctcc
    12541 gcagccttgt cgatatccga gtcgatacag tccaagaggg aaatccccat ggtgatggtc
    12601 cgaatatcga aattctgctc ctcaatcatt tcaatggttt ctctaacctg tctaatatcc
    12661 atgaaatctc tccttacaag ttgtgcatag catcaaaaat ggctgcactt tgaatgttga
    12721 ttttcacatt caaagcctga ccataggctt ctagttctgc acgaagcttg gtgaaatctt
    12781 tcttctcatc tgacgacacc accgccatca tggtaaaata ctcatccaaa acagtttgtg
    12841 aaatatcatc gatattgagc cctaactccg caatcttggt cgcaacaccc gcaacaattc
    12901 ctgacttgtc cttaccgaca actgtaac
//
LOCUS       DBNFCK010000051        12702 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.051, whole genome shotgun sequence.
ACCESSION   DBNFCK010000051 DBNFCK010000000
VERSION     DBNFCK010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12702
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.051"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(21..677)
                     /gene="phoU"
                     /locus_tag="AC1PIW_001127"
     CDS             complement(21..677)
                     /gene="phoU"
                     /locus_tag="AC1PIW_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HIP6606070.1"
                     /translation="MLRAQFEEELGKLHNQFYAMGNEVLAQINNAVRAFTIHDRELAK
                     EVIEADKKINAFEVKLEKKSLEIIALQQPVSTDLRRVITVLKATSDLERMGDHAVSVA
                     KAAIRMKGEVRIPVVEEEIKKMGREVRQLVEETLELYLGDADMERAYEVAARDEVVNE
                     FYSTIQELTTEEIKKNPESLVAGRDYYQVLTYLERVGDYAKNICEWVVYLRTGDITEL
                     "
     gene            complement(706..1464)
                     /gene="pstB"
                     /locus_tag="AC1PIW_001128"
     CDS             complement(706..1464)
                     /gene="pstB"
                     /locus_tag="AC1PIW_001128"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HIP6606071.1"
                     /translation="MTSPILQVKDLSVYYNKKKALNNVSIDFYPNEITALIGPSGSGK
                     STLLRSINRMGDLNPEVTLTGAIDYNGKNIYSPRTDTVDLRKEIGMVFQQPNPFPMSI
                     YENVVYGLRINGIKDKQVLDEAVERSLIGASIWNEVKDRLHDSAVGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEDTLYGLKDKYTMVLVTRSMQQASRISDRTG
                     FFLDGDLIEYNLTKEMFLNPANKETEDYITGKFG"
     gene            complement(1476..2279)
                     /gene="pstB"
                     /locus_tag="AC1PIW_001129"
     CDS             complement(1476..2279)
                     /gene="pstB"
                     /locus_tag="AC1PIW_001129"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HIP6606072.1"
                     /translation="MADYNWNERHIITFPEENIALSTKDVHVYYGKNEAIKGIDMQFE
                     KNKITALIGPSGCGKSTYLRSLNRMNDTIDIAKVTGQILYEGIDINRPDMNVYEIRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLKQAALWDQVKDDLHKS
                     AFTLSGGQQQRLCIARAISVKPQILLMDEPASALDPIATMQLEETMFELKKDYTIIIV
                     THNMQQAARASDYTAFFYLGDLIEYDKTANVFQNAKLKSTSDYVSGHFG"
     gene            complement(2304..3191)
                     /gene="pstA"
                     /locus_tag="AC1PIW_001130"
     CDS             complement(2304..3191)
                     /gene="pstA"
                     /locus_tag="AC1PIW_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIP6606073.1"
                     /translation="MHAKKFDKLATGILYSIAGVIVVILTSLILYILLRGLPHVSWDF
                     LTSKSSSYKAGGGIGIQLYNSFFLLVVTLLISVPLSTGAGIYLAEYAKKGPLTNFIRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSILSGALALTVFNLPQMTRNVEDSLRHVH
                     HTQREAGLALGLSRWETVLHVVIPEALPGIVTGIVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNPLSVTSPISIFRQSETLAVHIWKVNSEGTIPDATQVSAGSAAILLVFILIF
                     NLSARYIGKKLHAKLTSAA"
     gene            complement(3181..4098)
                     /gene="pstC"
                     /locus_tag="AC1PIW_001131"
     CDS             complement(3181..4098)
                     /gene="pstC"
                     /locus_tag="AC1PIW_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HIP6606074.1"
                     /translation="MKNEQLAKELSSPSKNSRLEKFGKVITFLCLSLIVFIVAMILIF
                     VAQRGLSTFFVDGVSITDFLFGSKWEPSSKIFGALPMISGSFIVTILSAVVATPIAIG
                     AAVFMTEISPKRGAKILQPVIELLVGIPSVVYGFIGLQVVVPFVRSIFGGTGFGILSG
                     VFVLFVMILPTVTFMTVDSLRAVPRHYREASLAMGATRWQTIWRVILNAAKPGIFTAV
                     IFGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSVLTMGIGNTVMGTVQNNVLWS
                     LALVLLLMSLIFNMIMKFITREGKKNYAR"
     gene            complement(4101..4970)
                     /locus_tag="AC1PIW_001132"
     CDS             complement(4101..4970)
                     /locus_tag="AC1PIW_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HIP6606075.1"
                     /translation="MKKKQKLGIAALFLLSSMALSGCSSWIDRGESITAVGSTALQPL
                     VEAAADEFGSVNIGKSINVQGGGSGTGLSQVQSGAVQIGNSDVFAEEKDGIDASKLVD
                     HQVAVAGLAVIVNEQVSVKDITTEDLRKIFTGEITNWKELGGKDLEISIINRAASSGS
                     RATFDSVIMDGQTATQSQEQDSNGMVKSIVAQTPGAISYLSFAYLDDSVKTIKLNGFE
                     PTAENVATNDWPIWSYEHMYTMGKATDLTEEFLDYMMSDEVQNGIVESMGYISINDMK
                     VVKSADGTVTEKE"
     gene            complement(5108..6409)
                     /locus_tag="AC1PIW_001133"
     CDS             complement(5108..6409)
                     /locus_tag="AC1PIW_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HIP6606076.1"
                     /translation="MQLPQDFIDKYNHLLGSEAQAFFESFEQDPISGFRTNPLKESQL
                     DFEQPIPNMTWSYYGKVSGKSPEHVTGLVYSQEPAAQVVGQIAAPKKGMKVLDLAAAP
                     GGKSTHLLSYLENTGLLVSNEISNKRARILAENIERFGARNVVVTNESADRLEKVFPG
                     YFDMIVLDAPCSGEGMFRKYPDAIQYWSKHYPAQCASLQREILESALNMLAPDGQLIY
                     STCTWSPEENEEVVTWLLEQYDLELIDIPKINGMVEGIGYTEVARMYPHHFAGEGQFV
                     AKFRFLGEASQKKLKPGKSNLNREQQSLWKEFEQKHLRVQLDGLLQVFGDNLYLLPHG
                     LPDLSKVKIARNGLHLGTYKKKRFEPSFALGLALHSSEVENRVEISLEEFSSYTAGHT
                     IAVSKDLANGWYQVAVQGNGLSFAKIVSGTLKNGFPKGLRF"
     gene            complement(6409..7167)
                     /locus_tag="AC1PIW_001134"
     CDS             complement(6409..7167)
                     /locus_tag="AC1PIW_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIP6606077.1"
                     /translation="METKYHFAQTIVLEAGNFLRQHLHDDLQIEEKGDFTDLVTHLDK
                     QVQEMLTQQIQSRYQGDGILGEEGGDVSSIHEGNVWVIDPIDGTTNFIAQKTDFAIMI
                     AYFENGVGKFGIIYDVMRDKLFHGGDSFPVFCNQERLKKPELRPLRQSLIGINAKLYA
                     GNAHGVAELANQSLGTRSVGSAGIGFAHVLEGRLFAYASYLCPWDYAAASIIGSSLGL
                     VMLSFEGPSLAYDKREFVMLVSESHLEEVKGYIF"
     gene            complement(7157..7435)
                     /locus_tag="AC1PIW_001135"
     CDS             complement(7157..7435)
                     /locus_tag="AC1PIW_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HIP6606078.1"
                     /translation="MNKNYSYPLDFSWSTEEISSVLSFLNQVEAAYEKGADAVAILAS
                     YKSFKDVVKSKGQERQIDRDFEAVSGYSTYRVVKAARDKGKGVICFGN"
     gene            complement(7450..7851)
                     /locus_tag="AC1PIW_001136"
     CDS             complement(7450..7851)
                     /locus_tag="AC1PIW_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HIP6606079.1"
                     /translation="MITLFLSPSCTSCRKARAWLTSHEVPFIEHNIMTSPLTAEELKN
                     ILALTENGTDDLISTRSKVFQKLDIDVDELSVKQLIGLIETYPSLLRRPIILDEKRMQ
                     IGFNEDEIRAFLPRKYRKQELKNATLRAEIG"
     gene            complement(7926..8672)
                     /locus_tag="AC1PIW_001137"
     CDS             complement(7926..8672)
                     /locus_tag="AC1PIW_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIP6606080.1"
                     /translation="MLKRLIRHPFYEKAMTYIAVVYVFLIVLELMDASIANSSGYRVL
                     DLLLWFIFVCDYFAQLYYADDILEYVQGHILELIAIIPFDSFFSFLRLGRLARLFRLV
                     KVTRIFALSNRFWDTINKLLHTNSLSKVLMLNISAVLVSSVLLSVIEGKSFFDALWWS
                     IVTMTTVGYGDIVPQDTISKIIAILLMLVGICTFGMVTSTITRFFSETERETKLDILM
                     AKIDEQNETLLRLEKKIESLGKLGELQGRD"
     gene            complement(8665..9588)
                     /locus_tag="AC1PIW_001138"
     CDS             complement(8665..9588)
                     /locus_tag="AC1PIW_001138"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HIP6606081.1"
                     /translation="MNIRTIKHYSELNQEEPTVLVLGYFDGLHLGHKALLDRARKVAD
                     EQGLTVTVLTFPESPRLAFSRFSPELLLHLTSQEQRYLLLKKYGVDNLILTDFTSEFA
                     NNTPQQFMERYIKGLNAQILVAGFDYHFGNCRADVKDLTELFDGQVEIVSEVSLGGEK
                     VSSTRIRQAIQSGDVSLANQLLGYPFMTEGIVVHGDARGRTIGYPTANLAPFDRVHLP
                     SEGVYVADVEVDGKRYRAMTSVGKNVTFDGTEMRIEAHIFGFHRFIYGEKITIFWLEK
                     IRDMVKFDGIEGLMEQMKSDEKFALQWGKYA"
     gene            complement(9623..10501)
                     /gene="truB"
                     /locus_tag="AC1PIW_001139"
     CDS             complement(9623..10501)
                     /gene="truB"
                     /locus_tag="AC1PIW_001139"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIP6606082.1"
                     /translation="MISGIINVKKEAGMTSHDVVFKLRKILQEKKIGHGGTLDPYVTG
                     VLPIAVGKATRMIEYMQEEGKIYEGEITIGYSTTTEDASGDLVERTPVLYIAEQAVDE
                     AMTSFVGTITQIPPMYSAVKVKGRKLYEYARAGEEVERPQRQIEIYSFERTSPIELAD
                     DCARFTFRVRCGKGTYVRTLSVDLGAKLGYASHMSKLERTGSAGMDLADALTLEEISS
                     LVAENDFSFLQPIERGIGDLPVVELKEEQVQDATFGRFVELNAEAERLAGFHDGQLIA
                     IFEKRDQLYKPSKVLV"
     gene            complement(10551..10760)
                     /locus_tag="AC1PIW_001140"
     CDS             complement(10551..10760)
                     /locus_tag="AC1PIW_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606083.1"
                     /translation="MDWPTIKIGDEGYYDAPLIFSWGYDEEAEESIDEAVAYEAAPSG
                     TMRRIHKELENLIGTTFGSYRFYDD"
     gene            complement(10732..10983)
                     /locus_tag="AC1PIW_001141"
     CDS             complement(10732..10983)
                     /locus_tag="AC1PIW_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606084.1"
                     /translation="MKQFERTYENIDFILFKAYYIEGKSVDVAVSLREQEYMIEYIGA
                     DNRVENSVDYTSIRISKAKKFREFLKGNQAVGLADHKNW"
     gene            complement(11010..12246)
                     /locus_tag="AC1PIW_001142"
                     /pseudo
     CDS             complement(11010..12246)
                     /locus_tag="AC1PIW_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364639.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
     gene            complement(12243..12476)
                     /locus_tag="AC1PIW_001143"
     CDS             complement(12243..12476)
                     /locus_tag="AC1PIW_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HIP6606085.1"
                     /translation="MNTIDLNLPVAEIINQHPEVKDILVELGFKPLANPAMLNTVGKV
                     TSLKTGSKMTKIPLDRIQQVLECNGYEVIGGEA"
     gene            complement(12489..>12702)
                     /locus_tag="AC1PIW_001144"
     CDS             complement(12489..>12702)
                     /locus_tag="AC1PIW_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histone acetyltransferase"
                     /protein_id="HIP6606086.1"
                     /translation="DAICQLASEFTDTVGIGVGLNANYGKAQRLYVKHGFIPDGSGVW
                     YRGCSLPVGAKAYNDDELALYFTKKL"
BASE COUNT         3725 a         2937 c         2310 g         3730 t
ORIGIN      
        1 agccctctcg gacttctgaa ttacaactct gtaatatcac ctgtgcgtag ataaacaacc
       61 cactcacaaa tatttttagc ataatcaccg acacgttcca agtaggttaa aacttggtaa
      121 tagtcgcgac cagcaaccaa ggattctgga ttcttcttga tttcttccgt tgtcaattct
      181 tgaatagttg aatagaattc attgacaacc tcatcacgtg ctgctacttc ataagcgcgc
      241 tccatatcag catcacctaa gtagagttcc aaagtttctt ctactaattg acgaacttca
      301 cgtcccattt tcttgatttc ttcttcaaca actgggatgc gaacttctcc cttcatacgg
      361 atagctgctt tagcaactga gactgcatgg tctcccatac gctccaaatc actagttgct
      421 tttaagactg tgataacgcg acgaagatct gttgagacag gttgttggag agcaatgatt
      481 tcaagtgatt ttttctctaa tttcacttcg aaagcattaa ttttcttatc tgcttcaata
      541 acttcttttg ctaattcacg atcatgaatt gtaaaggcac gaacagcatt attaatctga
      601 gccaatactt catttcccat ggcgtaaaat tggttatgaa gttttcctaa ttcctcttca
      661 aattgagctc ttaacatgtg aacctctttc tttttcttat aggccttagc cgaacttacc
      721 tgtaatatag tcttctgttt ctttattggc aggattcaag aacatttctt ttgtcaaatt
      781 gtattctatc aaatcgccat caaggaagaa acctgttcga tctgaaatac gtgatgcctg
      841 ttgcatagaa cgtgttacta gcaccattgt atacttatct ttcagaccat aaagtgtgtc
      901 ctcaatttta ccagctgaga tcgggtcaag ggctgaagtt ggctcatcca acaaaataat
      961 tttggggctt gttgccaata cacgggccac acatacacgt tgctgctgac cacctgaaag
     1021 tccaactgct gagtcatgca agcgatcttt cacttcattc caaatagaag caccaatcaa
     1081 ggaacgctca actgcttcgt ccaagacctg cttgtccttg ataccgttaa tacgtaaacc
     1141 ataaacgaca ttctcgtaga tagacatcgg gaatgggttt ggttgttgga ataccatacc
     1201 gatttcctta cgaagatcaa cagtatccgt acgaggactg tatatgttct ttccgttgta
     1261 atcaatcgca cctgtcaatg ttacctctgg gtttaaatct cccatacggt taatagaacg
     1321 caagagggtt gacttaccag aaccagaggg tccaatcaaa gcagtaattt catttggata
     1381 gaaatcgatt gacacattat tcaaggcttt tttcttgtta tagtaaacag acaaatcttt
     1441 tacttgtaaa ataggtgatg tcatttgttt cctttctatc caaaatgtcc tgaaacgtag
     1501 tcactagttg acttgagttt tgcattctgg aagacatttg ctgtcttatc atattcaatc
     1561 aagtcaccca gatagaagaa agctgtgtaa tcacttgcac gagctgcttg ttgcatattg
     1621 tgcgtaacaa tgataatggt gtagtccttc ttcaattcaa acatagtttc ttctaactgc
     1681 atagtcgcaa ttggatccaa ggctgatgca ggctcatcca taagcaagat ttgtggtttt
     1741 acagagatag ctcgtgcaat acacaaacgc tgttgctgtc cacctgataa ggtaaaggct
     1801 gacttgtgca agtcatcttt tacctgatcc caaagggctg cttgtttcag agaagtttca
     1861 acaatctcat ccaaaacctg cttatctttc acacctgcac gttcatgtgc gaatgtgatg
     1921 ttgcgataaa tcgattttgc aaatgggttg ggacgttgga ataccattcc gatatgctta
     1981 cgaatttcgt aaacgttcat atctggtcta ttgatgtcga ttccttcata aagaatttga
     2041 ccggttacct tagcaatatc aatagtatca ttcatacgat taagactgcg aagataggtt
     2101 gatttcccac accctgatgg tccaatcaag gctgtaattt tatttttttc gaactgcata
     2161 tcaatgccct tgattgcttc atttttacca tagtaaacat gaacatcttt tgtcgaaagg
     2221 gcgatattct cctctggaaa agtaatgata tggcgttcat tccagttgta atctgccatt
     2281 ttttctcctt ataagtcaga cttctaggct gctgaagtca gtttcgcatg aagtttctta
     2341 ccaatataac gtgcagaaag gttgaaaatc aagatgaaga ccaagagaat cgctgctgaa
     2401 ccagcagaaa cttgggttgc atctggaatt gtaccttcgc tgtttacctt ccagatatga
     2461 accgcaaggg tttctgattg acggaagata gaaatcggac tagtaacact caatggattc
     2521 cagtttgacc agtcaagagc tggagctgac tgaccagccg tgtagataag agctgctgcc
     2581 tcaccgaaga tacgaccaga tgccaagacg atacctgtta caatccctgg aagtgcttct
     2641 ggaataacaa catggagaac agtttcccaa cgtgatagac caagagccaa tcctgcttca
     2701 cgttgtgtat ggtgaacgtg acgaaggctg tcctctacgt tacgtgtcat ctgtggtaag
     2761 ttgaagactg taagagccaa ggcacctgat aagatagaaa aaccgtactc aaactgaaca
     2821 acgaaaatca agtaaccaaa caaaccaacg acaacggaag gtagggatga caaaatctca
     2881 atacaggtac ggataaagtt agtcaatgga ccttttttag catattcagc caaataaata
     2941 cctgcaccgg ttgacaaggg aacagaaatt aagagcgtta caaccaatag gaagaatgag
     3001 ttgtagagct gaattccaat accaccacca gctttgtaag acgaagattt acttgtcaaa
     3061 aaatcccagc ttacatgtgg cagaccacgt agcaagatgt aaagaatcag tgacgtcaag
     3121 ataacgacaa tcacaccagc gattgagtaa agaataccag tcgccaattt atcaaatttt
     3181 ttagcgtgca tagtttttct taccctctct cgtaataaat ttcataatca tgttaaagat
     3241 aaggctcatc aagagcaaga ctagggccaa ggaccatagg acgttgttct gaacagttcc
     3301 cataactgta ttaccgatac ccattgtcaa aacagaagtc aaagtcgctg ctggcgttgt
     3361 caaggaagtc gggataaccg cagagttccc cactaccatc tgaatagcca aggcttcacc
     3421 aaaggcacgc gccataccaa agataacggc cgtaaaaata cctggcttag ccgcgttcaa
     3481 aataacacgc caaatcgtct gccaacgagt ggctcccata gccaaacttg cttcgcggta
     3541 gtgacgaggt actgcacgta aactgtcaac cgtcataaag gttactgttg gcaaaatcat
     3601 aacgaaaagt acaaatacac ccgacaagat accgaaacct gttccaccga agattgaacg
     3661 tacaaatggc acaactactt gcaaaccaat gaaaccgtat acaaccgatg gaataccaac
     3721 caaaagttcg ataacaggtt gtaagatttt tgcaccacgt tttggtgata tttctgtcat
     3781 gaagaccgct gcaccgattg cgataggggt cgcaacaaca gctgacagaa tcgtaacaat
     3841 gaaagaacct gaaatcattg gcagggcacc aaatattttc gagcttggtt cccacttgct
     3901 tccaaataag aaatcagtga tgctcacacc gtcaacaaag aaagtcgata atccacgctg
     3961 tgcaacgaaa atcaaaatca ttgcaacaat aaagacaatc aatgatagac aaaggaatgt
     4021 aattacctta ccaaacttct ctaatcgaga atttttagat ggagacgata actcttttgc
     4081 tagttgttcg tttttcatgc ttactccttc tcagtcaccg tgccatcagc acttttcaca
     4141 actttcatat cgttgataga aatgtatccc atactttcaa cgataccatt ttgaacttca
     4201 tctgacatca tgtaatctaa aaattcttct gtcaaatcag tcgcttttcc catcgtatac
     4261 atatgctcat aggaccaaat tggccaatca tttgttgcta cattttcagc agttggttca
     4321 aaaccgttta acttaattgt tttaacggaa tcatctagat aagcaaatga taggtaggaa
     4381 attgcacctg gtgtctgagc aacaatagat tttaccatac cgttagaatc ttgttcttga
     4441 ctctgagttg cggtttgtcc atccatgata acactatcaa aggtcgcacg tgaacctgaa
     4501 ctagctgcac ggttaatgat tgaaatttcc aaatccttac cgcccagctc tttccagttg
     4561 gtaatctcac ctgtaaaaat tttacgcaga tcttctgtag taatatcttt aaccgatacc
     4621 tgctcattta caataacggc caaaccagct actgctactt gatggtcaac aagtttactt
     4681 gcatcaatac catctttttc ctcagcaaat acgtcagagt taccaatctg aacagctcca
     4741 gactgaactt gtgacaagcc agtacctgac cctcccccct gaacgttgat ggatttaccg
     4801 atattgacac taccaaattc atctgccgct gcctcaacaa gtggttgaag cgccgtcgaa
     4861 cctacggctg taatagactc tcctcgatcg atccaagaag aacaaccaga aagagccata
     4921 ctactcaaaa gaaacaaagc tgcaattcca agcttctgct tttttttcat ttcatttttt
     4981 cctttacatt tttcagtgga atctcctaaa attaactctg tattttacat agaatttaca
     5041 agatatttcc actcatccac tatatcatat aaaaagacaa gttgcaaaaa aaatttgctg
     5101 atttatttta aaatctcaac ccttttggaa agccgttttt aagcgttcca gatacaattt
     5161 ttgcgaaact aagaccgttc ccctggactg ctacctgata ccagccatta gctaaatcct
     5221 tggacacagc tatggtatgt cctgcagtat aggacgagaa ttcttctaag ctgatttcca
     5281 ctcgattttc aacctccgaa ctatggagtg ctagacctaa agcaaaactt ggttcaaatc
     5341 tttttttctt atacgtcccc aagtgaagac catttcgagc aatctttact tttgacaaat
     5401 caggaaggcc gtgtggcagt aggtagagat tgtcgccaaa aacctgcagt aaaccatcca
     5461 attgaaccct taaatgcttc tgttcaaatt ctttccagag agattgttgt tctctattca
     5521 aattagattt tcctggtttt aattttttct gactggcttc ccctagaaat ctaaatttgg
     5581 caacgaactg accttctcct gcaaaatggt ggggatacat acgagcaact tctgtatatc
     5641 cgattccttc taccattcca ttgattttag gaatgtctat tagttccaaa tcatactgtt
     5701 ctaagagcca agtcactact tcttcatttt cctctggtga ccaagtgcaa gtagaataaa
     5761 tcaattgacc atctggagcc aacatgttca aggccgattc taaaatttca cgctgtaagc
     5821 ttgcacattg tgctggataa tgtttggacc aatattgaat cgcatcgggg tattttcgaa
     5881 acatcccttc acctgaacag ggagcgtcta ggacaatcat atcgaaatat cctggaaaga
     5941 ccttttccaa gcggtctgct gattcatttg ttacaacaac attcctcgct ccgaaacgct
     6001 caatattttc agccaatatc cttgctcgtt tgttagaaat ttcattacta accaagagcc
     6061 ccgtattttc taagtatgaa agcaagtgag tagactttcc tcctggcgct gctgccaagt
     6121 ccaatacttt catccctttt ttcggtgctg caatctgacc tacaacctgt gcagctggct
     6181 cttgtgagta gactaagcca gttacatgtt ctggagattt ccctgagacc ttaccatagt
     6241 atgaccaagt catattaggg attggttgct caaagtccaa ttggctttct ttaagaggat
     6301 ttgtgcgaaa tcctgagatt gggtcttgtt caaaactctc aaaaaatgct tgggcttcac
     6361 taccaagcaa atgattatat ttatctataa aatcctgtgg taattgcatt agaatatata
     6421 ccccttcact tcctcaagat gggattctga tactagcatg acaaactctc gcttatcata
     6481 ggccagactc ggaccctcaa aacttaacat gactaagcct aaagaggaac caataatact
     6541 ggctgctgcg tagtcccatg gacacagata agaagcatag gcaaataatc ttccttccag
     6601 aacgtgggcg aaaccaattc cagcacttcc tacagatctt gtacctaaac tttgatttgc
     6661 taattctgcc actccatgtg cattgcctgc gtacaacttt gcattgattc ctatcaaact
     6721 ctggcgaaga gggcgcagct ctggtttctt taagcgttct tgattgcaaa aaacagggaa
     6781 tgaatctcct ccatggaaaa gtttgtctcg catgacatca tagatgatac cgaactttcc
     6841 tactccattt tcaaaatagg caatcataat ggcaaaatca gttttctgag caataaaatt
     6901 tgtggtgcca tcaattggat caataaccca aacatttccc tcatgaatag aagaaacgtc
     6961 cccaccttcc tcgcctaaaa tcccatcacc ttgatagcga gattgaatct gttgagtcaa
     7021 catttcttgc acttgtttat caaggtgtgt taccaagtct gtaaagtcac ctttttcttc
     7081 aatctgaaga tcatcatgta aatgctgtcg gagaaaattt ccagcttcca ggacaatcgt
     7141 ttgggcaaag tgatacttag tttccaaaac aaataactcc tttcccttta tctcgagcag
     7201 ccttaacaac tcgataggtt gaataaccag aaactgcttc aaagtcacga tcaatttgac
     7261 gttcctgtcc cttactttta acaacatctt tgaagctttt ataactagct aatatagcta
     7321 ccgcatctgc tcctttttca taagctgcct ccacctgatt caaaaaagaa agcacggagg
     7381 aaatctcctc tgtgctccac gaaaaatcaa gtggatatga ataatttttg ttcatatctt
     7441 ttatctttct tatcctattt ctgctctcaa tgtagcattt tttagttctt gtttgcgata
     7501 cttccgcgga agaaatgcac gaatttcatc ttcgttaaaa ccaatctgca tccgcttctc
     7561 atccaaaata atcggacgcc gtaagagact cggataggtt tcgattaacc caataagctg
     7621 cttcacagaa agctcatcaa catcaatatc tagtttctga aaaacttttg aacgggtaga
     7681 aatcaagtca tctgtcccat tttctgtcaa tgctaaaatg ttctttaatt cctcagcagt
     7741 tagagggcta gtcataatat tgtgctcaat aaaagggact tcgtgacttg tcagccaagc
     7801 acgagcttta cgacaactgg tacaactcgg tgacaaaaat aaagtaatca tgtcttccat
     7861 cccctctaaa aacatttttc ttcttattat tatactctaa cttaaaagag ttttcaatag
     7921 taaatctaat ctcttccctg taattcacct aattttccta aagattctat ttttttctct
     7981 aagcgtaata atgtttcgtt ttgttcatca atttttgcca tcaagatatc caacttcgtt
     8041 tctcgctctg tttcagagaa aaaacgagtt attgtggagg taaccatacc aaaagtacaa
     8101 ataccaacca acatcaagag gatggctata attttagaaa tcgtatcttg ggggacaata
     8161 tctccatacc ctacagttgt catcgttaca atggaccacc aaagtgcatc gaagaatgat
     8221 tttccttcaa ttactgataa taagacactt gaaactagca ctgctgagat atttaacatc
     8281 aacactttgg ataaactgtt ggtatgcaaa agcttgttga ttgtatccca gaatcgatta
     8341 gacagggcaa atattctcgt tactttgacc aatctgaaga gtcgtgcaag tcttcccaag
     8401 cgaagaaaac tgaagaatga atcaaatgga ataatggcaa ttaattccaa gatatgtccc
     8461 tgtacatatt caagtatgtc atccgcataa taaagctgag caaaataatc gcaaacaaaa
     8521 ataaaccata agagcaagtc tagtacccga tacccactag aattggcaat actagcatcc
     8581 attaattcaa gtacgataag aaatacatag actacagcta tgtaggtcat agccttctca
     8641 taaaaaggat gacgaatcaa tcgtttaagc atatttcccc cattgcaagg caaatttctc
     8701 atcagatttc atctgctcca tcaatccttc aattccatca aatttgacca tatctcgaat
     8761 tttttcaagc caaaaaatag ttattttttc tccgtagata aagcggtgaa acccaaaaat
     8821 atgggcttct atccgcatct ccgttccatc aaaggtcaca tttttcccaa cgcttgtcat
     8881 agctcgatag cgttttccat ctacttcgac atcagcgacg tagactccct cagatggtaa
     8941 atggacacgg tcaaaaggag ccagatttgc agtcggatag ccgatggtgc gtcctctggc
     9001 atctccatgc accacaattc cctccgtcat aaatggataa cctaaaagtt gattagctag
     9061 agagacatcc cctgactgga tagcctgacg aatacgagtt gacgaaactt tctctccgcc
     9121 caggctcact tcacttacaa tttctacctg accatcaaat aactctgtca aatctttgac
     9181 atctgctctg caatttccaa aatgatagtc gaaacctgca accaaaatct gggcattgag
     9241 acctttaata tagcgctcca taaactgctg aggagtatta ttagcaaatt cactagtaaa
     9301 atcagtcaaa ataagattat caacaccata ttttttcaat aagaggtagc gctgctcttg
     9361 actagtcaaa tgcagtaaaa gttctgggct aaagcgagaa aaagccaatc gaggtgactc
     9421 tggaaaggtc agaacagtta ctgtcaaacc ttgctcatct gctacttttc ttgctcgatc
     9481 aagtaaagct ttgtgcccaa gatgaagccc atcaaagtag cccagtacta aaaccgtcgg
     9541 ctcctcttga ttcaattcac tataatgttt aattgttcta atgttcatgt tttctatttt
     9601 atcacagtta ttgagaaatt tcttagacca aaaccttgct cggtttatac agctggtcgc
     9661 gtttttcaaa aatagcaatc aactgaccat catgaaagcc tgctaagcgc tctgcttctg
     9721 cattcaattc gacaaatcga ccaaatgtag catcttgcac ctgctcttct ttcaattcta
     9781 caacaggtaa atccccgata ccacgttcaa taggttggag gaatgaaaaa tcattttcag
     9841 ctaccagcga agaaatttct tctaaagtca aggcatccgc taggtccatt ccagctgatc
     9901 ctgttcgttc caacttggac atatggctgg catagcccaa cttagcccct aggtcaacag
     9961 acagggtgcg gacataggtg cccttaccac agcgaactcg gaaggtaaac cgtgcacagt
    10021 catctgctag ttcaatcggg ctagtccgtt caaaactgta aatctcaatt tggcgttgag
    10081 gacgttcaac ttcttcgccc gcccgtgcgt attcgtagag tttacggccc ttgaccttga
    10141 cagcagagta cataggcgga atctgggtaa tagtgcctac gaaactggtc atagcctcat
    10201 caactgcctg ctctgctatg tacaaaaccg gtgtccgctc aacaagatcg ccagatgcat
    10261 cttctgtcgt ggtagagtaa ccaatggtaa tctccccctc gtaaatcttg ccctcttcct
    10321 gcatgtactc aatcatgcgc gtggccttgc cgactgcaat gggcagaaca cctgtcacat
    10381 atggatccaa ggtcccaccg tgaccaatct tcttctcctg caaaatcttg cggagcttaa
    10441 aaacaacatc atgcgaggtc atacctgcct ctttttttac attaataatt cctgagatca
    10501 tgttctcatt ataacacaaa ggaccgctct tgtgggcggt ccggtatcaa tcagtcatcg
    10561 tagaaacgat agctaccgaa tgttgtacct atcaaattct ctagttcctt atgaatacgt
    10621 cgcatggttc cagaaggtgc tgcttcatat gcaactgcct catcaatgct ttcctcagcc
    10681 tcctcatcat agccccaaga aaatatcaat ggggcatcat aatagccttc gtcaccaatt
    10741 tttatggtcg gccaatccaa cagcttgatt tcctttcaaa aattctctaa atttttttgc
    10801 cttgctgata cgaatagaag tgtaatctac tgaattctca actcgattat ctgcacctat
    10861 atattcaatc atatactcct gctccctaag actgaccgca acatctacgc tctttccttc
    10921 aatataataa gccttaaaaa gaataaagtc aatattctcg tatgtacgtt caaattgttt
    10981 catgttcttt cctttcaaaa atgactaaac tagatatccc gatgaaaatc gctatcaatc
    11041 tcaaaaaatg gttgaatatc ctgaacatac tccaaaacgc cttgaaactc tccttggtca
    11101 tcacgcaccg ccttatagac tacatagaca aacttgccca tgctctcgga cttgaaccac
    11161 atggcgtcca atctggcttg gtgttcgttt gaaaatcatg tcttcgacag ggtggctgtc
    11221 attgtaatat tggaaaatgt catccttgtt gacaaaggtt aattccagcg gtaaatgatt
    11281 gaggataaga ttcgcttgct caacagataa ataaccattg ccgaaggtct gcggactagt
    11341 tctgtcctga acagtttctg acttttcctt gggtgtgaag gcaatggtaa actggccttc
    11401 tggcgtgtcg ataatctgct gatttggtcc tgctggctgc agagaaacag catttacttc
    11461 ctgctctgct atttcccaaa aagcctcccg atgaggtacc cacttggctg tcggcttgac
    11521 aatggcgtag ccgtaggcgt cactgtctac tgccacttgg agccagtcgt cctgggtgaa
    11581 aatctccagc aaaatcatga gaagtatggc ttcttccttg aaaatcatct cctcgaactc
    11641 cttggcgaag ttttcaaagt gagcacgtgt cgcagctagg tcacctcctt caagtgcctt
    11701 acgggccgtc ttgaagagat cgcgaatctc gtcgtccaca ccccacatga ccttgggcgg
    11761 tgcatcataa ccgtaccgct ccataatggg aaagaagacc ttttccttgc gggtatagtg
    11821 gttttcaaat tgaccgagga ggccaaactg gcgtgtcaaa ccttgcagga gctggtcttt
    11881 cagctccgca tcctctgtct gctcatactg gtcaatgata cggcggatgc gtaagagagc
    11941 tgcccgcagg gcaaggtttt cctgcttaaa gacatagaca gggtgtcctt cccggtccat
    12001 atccgccaca tccacatccg caatggctcc tttgaagagg ttggcgtgga tattgcagag
    12061 gctcatgaca tcttcaaaag tgatacccgt atcgctggac atgagttcat gctccatcat
    12121 ggagatttcc aaggcagaaa ctcccgtgaa atgctgatta aactggtcct gaacagactc
    12181 tggactggcc ccattgtgca ggtccaagag aatattcttc agaatgtcaa cacgtttttc
    12241 ggtcatgctt ctcctcctat tacctcgtag ccgttgcatt ccaagacctg ttgaatacgg
    12301 tctagtggaa tcttggtcat cttagatcct gtcttcagac tggttacctt accaactgtg
    12361 ttcagcatag ctggatttgc caagggcttg aaaccaagct ctaccagaat gtccttgact
    12421 tctgggtgct ggttgataat ctctgcaaca ggcagattta ggtcgatggt gttcatttta
    12481 cttttcctct ataatttttt tgtaaaatac aaggccaatt catcatcgtt ataggctttg
    12541 gcacctactg gcaaagagca ccctcggtac cagacaccgc ttccgtctgg tatgaaacca
    12601 tgttttacat agagccgttg agcctttcca taatttgcat tcaaaccaac tccaatacct
    12661 actgtatccg taaattctga ggcaagttgg cagatagcat ct
//
LOCUS       DBNFCK010000052        12605 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.052, whole genome shotgun sequence.
ACCESSION   DBNFCK010000052 DBNFCK010000000
VERSION     DBNFCK010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12605
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.052"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..564
                     /locus_tag="AC1PIW_001145"
     CDS             <1..564
                     /locus_tag="AC1PIW_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HIP6606087.1"
                     /translation="EVGKDFTTLSIHYWKEQTGLIKDRFSQLIGDASVLSDQKRAAIK
                     EIIFQFQQPVLYHDSKQLFDQKSLVEGFRLGDIQIFGDANKIDLQKLEKRYNSLLKTA
                     YKHINEQLISHHQATFYKWVEALLHLVTENIVEFSPDLHSTNLLIQDNRVKIEELSTN
                     LQNLHEYSKDLHDLMAWKDDEEVANGY"
     gene            554..3892
                     /locus_tag="AC1PIW_001146"
     CDS             554..3892
                     /locus_tag="AC1PIW_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIP6606088.1"
                     /translation="MVIKKMIKKVATKAADGVSKLSELSPEQLEKVQAQMATYRDQIP
                     DLSGEAAEEYFRKQLAIAGVEVYNAYLPQLKDLYLPIDNKTEFRLEDEGVLIDEGEFK
                     SGYNIRYINITKWVTDKQENSLEKLVSVYEVLSTTPCNISLVFHRKQEETSVYLAVTN
                     TENADSNADVDSYRKRLVSAIKGNFPGAAWKEEVGRGSLPCFEDYIPYSVATASNIPT
                     EKSEKFISQTIEKLLDGIVPENKEQEYILVLLATPVLDVTDRKLHLSQIYSNLSPYAT
                     WQTQFQLTESDSRGSSATVGVNIGASVGSQTGHNLTQTHSQGQTESRGTSETETNTKG
                     SGWQAGVNIKILNGGHNWNKSKSIAKGITNTIGKTASVANAVGKTVSSSLGANFGANF
                     ARTSSVTATIGKNEGISQSFVNHHVQHALKNLDKQMERLELSTALGLWDFSAYVLSED
                     PTIANNVAHSYLALTQGEESHLSQTVVNLWRGDVQMERDKAKALIAYLRDLRHPLFGF
                     NPEILEIDEEFAVYPEIVTAATPLSGKELAYALNFPQKSIAGLPVLECAQFGRNISTF
                     EEKDDQEQLPLGNIFHMNHEENTAVQLSLPSLASHTFITGSTGTGKSNTVYQLLAQAR
                     EKGIKFLVVEPAKGEYKQVFGSDEDVAVFGTNPTITPLLKINPFSFPEGIHILEHLDR
                     LVELFNVCWPMYAAMPAVLKKAIEQAYQDCGWDLISSVNTYSKTIFPTFADVLRTIRE
                     IIDSSEYDNDNKGAYKGALLTRLESLTTGIYQLMFTEDEISSEKLFDENVIVDLSRLG
                     SSETKSLIMGLLILKMQEYRMVSSQGMNQQLRHVTVLEEAHNLLKRTSTEQATESSNL
                     LGKSVEMLTNAIAEMRTYGEGFIIADQSPGLLDLAVIRNTNTKIILRLPEYSDRELVG
                     KSANLNEEQINELAKLPKGVAAVYQNEWLEPVLCKVAKYDSTEMVFQQPTQLVRTESP
                     YHRLAILELLTTSPNLDSTLSFEELQELTLAVGLDSLTQVRIFNFLRTTSPDNFSMHE
                     LAHILGKVYPELVEKIRLKAEKGLEPPALTQVLQQEIDSSIPETISEQARRDLVQGLM
                     TCYYLFELKDHKAMQDWLRNGDLI"
     gene            3889..4488
                     /locus_tag="AC1PIW_001147"
     CDS             3889..4488
                     /locus_tag="AC1PIW_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH/ENDO VII family nuclease"
                     /protein_id="HIP6606089.1"
                     /translation="MIEGLKTVAEAVKPVVNEVGKRAIDAFEKPIDSDKRLEAASDNK
                     TTFRYDGLSKPQEFLAQAQGFPKEIAKDIYNSQELKHYMDIGMRAVQFGQKENGEPRF
                     CLIPKDLSFDRVVDSQGRTNAERLQSGLLPIKDGQPLEVHHIGQKNGGHYALLTKEEH
                     IKNGNKEMLHKPGKSDVDHGLDFARDKRSIAKVLMEEQV"
     gene            4485..4895
                     /locus_tag="AC1PIW_001148"
     CDS             4485..4895
                     /locus_tag="AC1PIW_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HIP6606090.1"
                     /translation="MSQLVDKIKVVQGLYSGSPASEQEVAVAESKLQLIFPAEYKDYL
                     KEYGVISFYGTEWNGLKGDTWTDVVATTLEARSLYENFPKEKFILEDLHFDDMLVLAD
                     STGKVFLWHNGLEKEIHSSIASYLEECVARKDTP"
     gene            4892..5302
                     /locus_tag="AC1PIW_001149"
     CDS             4892..5302
                     /locus_tag="AC1PIW_001149"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIP6606091.1"
                     /translation="MIQPIMKDIFFLQQKSEPATQLDVQVGQDLQDTLAANAHACVGM
                     AANMIGVKKRIIIVNMGFTNLVMYNPVLISKAKPYQTEEGCLSLEGTRPTTRYQEIEV
                     EFFDASWKKISLKLTDFQAQIVQHELDHLEGIII"
     gene            5607..7706
                     /locus_tag="AC1PIW_001150"
     CDS             5607..7706
                     /locus_tag="AC1PIW_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIP6606092.1"
                     /translation="MNNNFNNFNNMDDIFNQLMGNMGGYSTERRRYSINGREVTPEEF
                     AMYRQTGRLPQTEEVAQTQAKGQIKSDGILAKLGRNLTQDAREGKLDPVIGRNKEIQE
                     TSEILARRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGLEA
                     GTQYRGAFEENVQNLVDEVKKAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKPAL
                     SRGELTVIGATTQDEYRNTIHKNAALARRFNEVKVNAPSAEDTYQILKGIKPLYEAHH
                     NIELPDEVLRAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDIQTLEAEMAE
                     AKQLQLEAAEKEDYEKALNEKVRIDKLQKQIDNHTEQQKVVATVNDVAQAVERMTGIP
                     VSQMGASDIERLKELKNRLAANVIGQDDAVEAVSRAIRRNRAGFDDGNRPIGSFLFVG
                     PTGVGKTELAKQLALDLFGNKDAIIRLDMSEYSDRTAVSKLIGTTAGYIGYDDNSHTL
                     TERVRRNPYSIVLMDEIEKADPQVITLLLQVLDDGHLTDGQGNQVNFKNTIIIATSNA
                     GFGYGMAEGEENQDIMDRIAPFFRPEFLNRFNAVIEFKHLDKDDLKAIVELLLAQVNN
                     TLAKKGIQLTVTEAAKEFLMEEGYDKAMGARPLRRVIENQIRDKVTDFYLDHVDVKYL
                     EADVVDGSIQIKEQSFA"
     gene            7889..9397
                     /gene="malQ"
                     /locus_tag="AC1PIW_001151"
     CDS             7889..9397
                     /gene="malQ"
                     /locus_tag="AC1PIW_001151"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HIP6606093.1"
                     /translation="MTNRTSGILMHITSLPGKFGIGTFGQSAYDFVDFLVETKQTYWQ
                     ILPLTTTSYGDSPYQSFSAIAGNTHLIDFDLLVEDGLLATEDFQDVNFGDNPEKVDYA
                     LIYQVRRPILEKAVQAFLQDDKKKAAFLEFEKANSSWLTDYAEFMAIKEYFGNKALQE
                     WEDKKVVARNEEALDKYRLELANQIDYYKVTQYFFFSQWKQLKEYANKHHIKIIGDMP
                     IYVSADSVEVWTKPQLFKLDSERKPLYVAGVPADNFSADGQLWGNPIYDWPEHEKTGY
                     NWWIYRIHESFKLYDVLRIDHFKGFSDFWQVDGKAEVAKVGTWEPGPGYNLFKAVKET
                     LGDLPIIAEDLGNIDAKARKLLADCGYPGMKILEFGFFDVTGKSIDAPHRCIPNSVAY
                     TGTHDNEVVNGWYNNLDPEQQEYVDAYSNRKPIEKVSQAMLRMLFATVSDTAIATMQD
                     VLDLGEESRMNMPSTIGGNWEWRMKAEDLTQERKDFLTKMTLLYQRGNENHD"
     gene            9390..11654
                     /gene="glgP"
                     /locus_tag="AC1PIW_001152"
     CDS             9390..11654
                     /gene="glgP"
                     /locus_tag="AC1PIW_001152"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HIP6606094.1"
                     /translation="MTKFTTFAETNTSKKLADLTNEEIYLQLLNYVKDAAASKPKNTG
                     KRKVYYISAEFLIGKLLSNNLINLGVYKDVQAELAAAGKSLAQVEDVEPEPSLGNGGL
                     GRLASCFVDSMSTLAINGEGVGLNYHCGLFRQVFKKNEQEAEPNFWIENDSWLIPTTI
                     SYDVPFKNFTLKSKLDRLDILGYKKETKNYLNLFDIESVNYDLITDGISFDKTDIKEN
                     LTLFLYPDDSDKNGELLRIYQQYFMVSNAAQLLIDEAIERGSNLHDLADYAYVQINDT
                     HPSMVIPELIRLLTEKHGIEFAEAVAIVKNMTGYTNHTILAEALEKWPLEFLEEVVPH
                     LVDIIKELDALIRAEIKDPAVQIIDESGRVHMAHMDIHFSNSVNGVAALHTEILKNSE
                     LKAFYELYPEKFNNKTNGITFRRWLEFANQDLADYIKELIGDEYLTDATKLEKLLAFA
                     DDKEVHAKLAEIKFNNKLALKRYLKDNKGIELDENSIIDTQIKRFHEYKRQQMNALYV
                     IHKYLEIKNGNLPKRKITVIFGGKAAPAYIIAQDIIHLILCLSELINNDPEVNKYLNV
                     HLVENYNVTVAEKLIPATDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIA
                     ELAGMDNIYTFGKDSDTIIDLYDKAGYVSADYYNGDANIKRAVDFIVSDEVKALGNEE
                     RLGRLHHELISKDWFMTLIDLAEYIEVKEQVFADYEDQDSWNKKVVHNIAKAGFFSSD
                     RTIEQYNEDIWHSN"
     gene            complement(11882..12589)
                     /locus_tag="AC1PIW_001153"
     CDS             complement(11882..12589)
                     /locus_tag="AC1PIW_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018367901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HIP6606095.1"
                     /translation="MSKYKKVYQDIKKKIEEQVWSTGQALPTENELMEVYSYSKDTIR
                     KALSLLEMDGYIQKKQGKSSIVIEHGLMKDQYLSEIKTAGELNKRSQHQIQTQLTSLY
                     IIQGQEDLMSTFEVDDKVDFYRVSRVRTIDGERLEYDISYFDRRVVPYLSKEIAESSI
                     YRYLEEELHLTISHSRREISFRFANEEEKQLMDLGDYDMVVVVTSITYLSNGQAFQYG
                     TISYRPDKVSFVSMAKR"
BASE COUNT         3990 a         2381 c         2727 g         3507 t
ORIGIN      
        1 gaggttggaa aagactttac gactctgtca attcattatt ggaaggagca gacaggattg
       61 attaaagata gattttctca gctcattggt gatgcgtccg tattgtcaga tcaaaagcgg
      121 gcggctatta aagaaatcat tttccaattc cagcagccag ttttgtacca tgattccaag
      181 caattatttg atcaaaaatc attggtagaa ggattccgtt tgggagacat ccagatattt
      241 ggtgatgcca acaagatcga tttacaaaag ctagaaaaac gctataatag tttgttaaaa
      301 actgcttaca agcatatcaa tgagcaattg attagtcacc atcaagcgac tttttataaa
      361 tgggttgaag ccttattgca tttggtaacg gagaatattg tagagtttag tccagacctt
      421 cattctacga atttgttaat acaggacaac cgagtgaaga ttgaggaatt atcaaccaac
      481 ttgcaaaatc tacatgagta ttctaaggac ttgcacgact tgatggcatg gaaagatgat
      541 gaggaagtag caaatggtta ttaagaagat gataaaaaaa gtagctacca aggcagcaga
      601 cggtgtttca aaattatcag aattgagtcc agagcagctt gaaaaagtgc aggctcaaat
      661 ggcgacttat cgggatcaaa ttcctgattt gtcaggtgag gctgcggagg aatactttag
      721 gaaacagtta gcaattgctg gcgtagaagt ctataatgcc tatctacctc agttaaaaga
      781 cctttatctt ccgattgaca ataaaacgga atttcgttta gaagatgagg gagttttgat
      841 cgatgaaggc gaatttaaga gtggttataa tatccgctac atcaacatta ccaagtgggt
      901 gacggataag caggaaaata gtttggagaa actggtcagt gtttatgagg tcttgtcaac
      961 aactccttgt aatatttctc ttgttttcca ccgtaaacag gaggaaacat ctgtatattt
     1021 agcggtcact aacactgaaa atgcggatag taatgcagat gttgatagtt accgcaagcg
     1081 attggtctca gctatcaagg gaaattttcc aggtgcagct tggaaagagg aggttggaag
     1141 aggtagcctg ccttgttttg aggactatat tccctactca gtagcaacag cttctaatat
     1201 tccaacggag aaatcagaga agttcatcag tcagacaatc gaaaaattat tggatggcat
     1261 tgtacctgaa aacaaagaac aagaatacat tcttgtcctc ctggcaacgc cagttttgga
     1321 tgttacagat agaaaattgc atctttctca gatttattct aacctatctc cttatgccac
     1381 ttggcagacc cagttccaat tgacagaatc agattccaga ggctcttctg caactgttgg
     1441 ggttaatatt ggtgccagtg tcggaagcca gacaggtcac aatctaaccc aaacccattc
     1501 gcaaggtcaa actgagtcaa gaggaactag tgagacggag accaatacca aaggaagtgg
     1561 ttggcaagca ggtgtaaata ttaaaatact caacggaggt cataattgga acaaaagcaa
     1621 atcaatcgcc aaaggcatca ccaatacaat aggcaaaaca gcctcggttg ccaatgccgt
     1681 tgggaaaaca gtttctagca gtctaggtgc caattttgga gctaattttg ctcgaacttc
     1741 cagtgtgact gctaccattg ggaaaaatga aggcattagt cagtcctttg tcaaccacca
     1801 tgttcagcac gctctaaaaa atttagacaa gcagatggag cgtttggaat tgtctactgc
     1861 cttgggacta tgggattttt ctgcctatgt tttgagtgaa gatccaacta ttgccaataa
     1921 cgttgcccat tcctatttgg ccttgactca gggagaagaa tcgcatctgt cgcaaacagt
     1981 tgtcaatctt tggcgtgggg atgttcaaat ggaacgtgat aaggctaaag cactgattgc
     2041 ctacttgaga gatttacgcc accctctctt tggtttcaac ccagaaattc tagagattga
     2101 tgaagaattt gcagtttatc cagaaatcgt cacagcagcc acgccactgt ctggaaagga
     2161 attagcctat gctctcaatt ttcctcagaa atcgattgcc ggtcttcctg ttttagagtg
     2221 tgctcagttt ggtcgtaata tctctacttt tgaggagaaa gatgatcagg agcaacttcc
     2281 tttgggaaat attttccaca tgaatcatga ggaaaataca gcagttcagc tttccttacc
     2341 ttccttggcc tcccatacat ttattacagg tagtacaggt acaggtaaga gtaataccgt
     2401 ctatcaattg ctcgctcagg ctagggaaaa aggaattaaa tttttggttg tagagccagc
     2461 taaaggagaa tataagcagg tttttggctc agatgaggat gtggcagttt ttggaaccaa
     2521 tccaactatc actcccttat taaaaataaa tccattcagc ttccctgaag gcattcatat
     2581 cctagaacat ttagaccgtc ttgtagaact cttcaatgtt tgttggccaa tgtatgcagc
     2641 gatgcctgct gttttgaaaa aagccattga acaagcctat caagattgtg gctgggattt
     2701 gatttcgtca gtgaatactt attcaaaaac cattttccca acgtttgcag atgtcttacg
     2761 gacaatcaga gaaattatcg actcgagcga atacgacaat gataataagg gagcctataa
     2821 aggagctttg ctgacacgct tggagtcact gacaacgggg atttaccagt tgatgtttac
     2881 agaagatgag atttcatccg agaagttatt tgatgaaaat gttatcgtgg atttgagtcg
     2941 tctgggttct agtgagacca aatcgctgat aatgggtctc ctcattttaa aaatgcaaga
     3001 ataccgaatg gtttctagtc agggtatgaa tcagcagctc cgccatgtga ctgttttaga
     3061 agaggcccac aatctcttga aacgtacatc aacagaacag gcaaccgaat ctagtaattt
     3121 acttggaaaa agtgtggaga tgttgaccaa tgccatcgct gagatgagga catatggaga
     3181 gggctttatt atcgcagatc agtcgccagg tctcttagac ttagctgtta ttcgtaatac
     3241 caataccaag attattttgc gcttacctga atattccgat agggagttgg ttggtaagtc
     3301 tgctaatcta aatgaagaac aaataaatga attggccaaa ctgccgaaag gtgtcgcagc
     3361 agtctatcaa aatgaatggc tggaaccagt tttatgtaag gtggcaaagt atgactcaac
     3421 tgaaatggta tttcaacaac caacccaatt agtaaggaca gaatcaccct atcaccgctt
     3481 ggctatttta gaattactga ctactagccc aaacctagat agcaccttgt cttttgaaga
     3541 attacaagaa ttaactctag cagtaggtct tgatagtttg actcaagtaa gaatctttaa
     3601 cttcttacga acaacatccc cagataattt tagtatgcat gaactggcgc acattctcgg
     3661 gaaagtatac ccagaattag tcgagaaaat tcggttgaaa gcagaaaaag ggttagagcc
     3721 acccgctttg acacaggtac ttcagcagga aattgacagt agtatacctg agacgatcag
     3781 tgagcaggcc agacgggatt tagtacaggg cttgatgact tgttattatt tatttgaact
     3841 aaaagaccac aaggccatgc aggattggtt acgaaatgga gacttaatat gatagaagga
     3901 ttgaaaactg ttgcggaggc agttaagcct gtcgtaaatg aagttggcaa acgagcgata
     3961 gatgcctttg aaaagccgat agattcagat aagcgattgg aagctgcatc tgataataaa
     4021 accacttttc gctatgatgg tctgagcaag cctcaagaat ttctagctca agcccaaggg
     4081 tttcccaaag agatagctaa ggatatttac aattctcagg aactcaagca ttatatggac
     4141 ataggaatgc gagctgtgca atttggacag aaagaaaatg gggagccacg attttgtctt
     4201 attccaaaag atttatcgtt tgatagagtt gtagactcac aggggcgaac caacgcagag
     4261 cgattgcaaa gtggattact accgataaag gatggtcaac ctttagaagt tcaccatatc
     4321 ggtcaaaaaa acggtggtca ttatgcctta ttgacgaaag aagaacacat taagaatggg
     4381 aataaagaaa tgttgcataa gccaggtaaa tctgatgttg atcatggttt agattttgct
     4441 agagataagc gatcaattgc taaagtactt atggaggaac aagtatgagt cagttagtag
     4501 ataaaataaa agtagtccaa ggtctttata gcggtagccc tgctagtgag caagaagtgg
     4561 cagtggctga gagtaagctt cagttgatat ttccagcaga gtataaggac tatttgaaag
     4621 agtatggggt gatttcattc tatggaacag aatggaatgg attgaaagga gatacttgga
     4681 ccgatgtagt tgcaaccacc ttggaagcgc gtagtctgta tgaaaatttt cctaaagaga
     4741 aatttatttt agaggatcta cattttgatg atatgcttgt tttagcggat tcgactggta
     4801 aagtcttttt gtggcacaat ggattagaaa aagagattca ttcctcaatt gcgtcctatc
     4861 tggaagaatg tgtagccaga aaggacaccc catgattcaa cctattatga aagatatttt
     4921 cttcctgcag cagaagtctg agcctgcgac tcagctagat gtgcaggtcg gtcaggactt
     4981 gcaggacact ttagcagcca atgctcatgc ctgcgtgggc atggcggcca acatgattgg
     5041 cgtcaaaaag cggattatca tcgtcaatat gggttttacc aacctggtca tgtacaatcc
     5101 cgtcctcatc agcaaggcca agccctatca gacagaagag ggctgcctgt cgctggaagg
     5161 tactcgcccg acgacacgct atcaggagat tgaagtggag ttttttgatg cttcatggaa
     5221 aaagatcagc ctaaagctga cagacttcca agcccagatt gttcagcatg aactagatca
     5281 tttggaaggg attatcatct aaatctaccg tcaggtagat ttttttgtat aaaagtcaaa
     5341 aaaggtcaaa caaaactatt gacttttact gaccaatgat gtaaaataag attcgtaagg
     5401 taaagggcct tacaaattct ttgaacaaaa ggtcagttta ggtcaaacaa aaaagtttga
     5461 ccgacaaatt gactaaatgg tcagtaaaag acaaacttct aaggaggttg ctatgaaaga
     5521 caggattata ccctattaac tattttttta tcacaaaaag gtcagttttg gtcaataaaa
     5581 acataacatg aaacgaggta aaaactatga acaacaattt caataacttt aacaatatgg
     5641 acgacatttt caatcaactc atgggcaaca tgggtggcta cagcacagaa cgtcgccgtt
     5701 attctatcaa tgggcgtgaa gtgacaccag aagaatttgc tatgtaccgt cagactggtc
     5761 gcctgccaca gacagaagaa gtggctcaaa cacaggctaa aggtcagata aagtcagacg
     5821 gaattcttgc aaaacttggt cgcaatttga cacaagatgc gcgtgaaggt aagttggatc
     5881 cagtcattgg acgaaacaag gaaatccaag aaacctctga aatcctagct cgccgtacga
     5941 aaaataatcc tgttttggtc ggtgatgctg gtgtcggaaa gacagcggta gtagaaggat
     6001 tggctcaagc cattgtcaat ggtgatgtgc cagcagccat taagaacaag gaaatcatct
     6061 ccatcgacat ttcaggcttg gaggctggga ctcagtatcg tggagctttt gaggaaaatg
     6121 tgcagaatct tgtggatgaa gtcaaaaaag ctggaaatat cattctcttc tttgatgaaa
     6181 ttcatcagat tttgggagct ggtagcactg gcggagactc aggttccaag ggtttagcgg
     6241 atattctcaa accagcttta tcccgtgggg aattgacggt tatcggtgcc actacacagg
     6301 acgaataccg caatactatt cacaagaatg ctgctctggc tcgtcgtttc aatgaagtca
     6361 aggtcaatgc tccgtccgca gaggacacct accagatttt gaaagggatt aagccgcttt
     6421 atgaagccca ccacaatatt gaattgccag atgaggtttt gagagctgcg gttgattatt
     6481 ccgtccaata tattccacaa cgtagcttgc ctgataaggc tattgaccta gtcgatgtga
     6541 ccgctgcaca tttggctgca caacatcctg tgacagatat tcagacctta gaagcagaaa
     6601 tggctgaggc aaaacagttg cagttggaag cagctgaaaa agaggattat gaaaaagcct
     6661 tgaatgaaaa agtccgtatt gataagctac aaaaacagat tgataatcat acggagcaac
     6721 agaaagtggt ggcgactgtt aatgatgtgg ctcaggcggt tgagcggatg acaggaattc
     6781 cagtctctca aatgggagct tctgacattg aacgactcaa ggaattgaaa aatcgtctgg
     6841 cagcaaacgt tatcggtcaa gacgatgcgg tggaagctgt atctcgtgcc attcgtcgga
     6901 atcgtgcagg ttttgatgat ggcaaccgtc caattggttc cttcctcttt gttggtccga
     6961 caggtgtggg taagacagag ttggcaaaac agttagcact agacttgttt ggtaacaagg
     7021 atgccattat tcgtttggat atgtctgaat acagcgatcg gacagccgtt tccaaattaa
     7081 tcggtaccac agctggctac attggctacg atgacaattc ccatactctt accgagcgcg
     7141 tccgccgcaa tccgtactca atcgttctta tggatgagat tgaaaaagct gatccacaag
     7201 tgattaccct tctcttgcaa gtgctagatg atgggcattt gactgatgga cagggcaacc
     7261 aagttaactt taaaaatacc attattatcg cgacttctaa tgcaggtttt ggttatggaa
     7321 tggcagaagg tgaagaaaat caagacatca tggaccgcat cgcaccattc ttccgcccag
     7381 agtttctcaa tcgttttaac gcagtcatcg agttcaagca tttggataaa gatgatctca
     7441 aagccattgt agaattactc ttggcccagg tcaataatac cctggcgaag aaaggcatcc
     7501 aattaacggt aacggaagct gccaaagaat tcttgatgga agaaggctat gacaaggcca
     7561 tgggagcccg tcctctgcgc cgtgtgattg aaaatcagat tcgtgataag gtgacggatt
     7621 tctatttgga tcatgttgat gtcaaatatc tggaagcgga tgtggttgat ggaagcatcc
     7681 aaataaaaga gcaaagcttt gcttaaaaat agtgaagaat ttgaaaaatc ggatagaaat
     7741 atctgatttt ttcttttttg ttattcgcta acaaatgcta taaaataggc tttctagagc
     7801 acattaaaac tttttttata aaatccttga caagtgcaaa cgtttgcgtt aaactatatt
     7861 cgtaaccgac agaaaggaaa acgtttttat gacaaatcgt accagtggaa ttttgatgca
     7921 tattacctca cttccaggta agtttggtat tggtactttt ggacagtctg cctatgattt
     7981 tgtagatttc ttggtggaaa ccaagcaaac ctactggcag attttaccac taacaactac
     8041 aagctatgga gattctcctt accagtcttt ctctgctatt gcaggaaata cacacttgat
     8101 agactttgac ctcttggttg aagatggctt attggcaaca gaagatttcc aagatgtaaa
     8161 ttttggagat aatcctgaaa aagtagacta tgctcttatc tatcaagttc gtcgaccaat
     8221 tttggagaag gcagttcaag catttttaca agatgataag aagaaagctg cttttctaga
     8281 gtttgaaaag gctaactcat cttggttgac agattatgcg gagttcatgg ctatcaagga
     8341 atactttggt aataaagctc tccaagaatg ggaagataaa aaagttgtag ctcgcaatga
     8401 ggaagctctt gataagtacc gcttagaatt ggcaaatcaa atcgactact acaaagtcac
     8461 acagtatttc ttcttcagcc aatggaaaca attgaaagaa tatgccaaca aacaccacat
     8521 caaaatcatc ggagatatgc caatctatgt ttctgctgat agcgttgaag tgtggacaaa
     8581 accccaacta ttcaaattgg acagtgaacg caagccgctt tatgtagcag gtgtaccagc
     8641 tgataacttc tcggcagatg gtcaattatg gggaaatcct atttacgatt ggccagaaca
     8701 tgaaaaaaca ggctataact ggtggattta tcgtatccat gaaagtttca aactttatga
     8761 tgtccttcgg attgaccatt tcaaaggttt ctcagacttc tggcaagttg atggtaaggc
     8821 agaggtggca aaagttggaa cttgggaacc aggtcctggc tacaatctct tcaaggctgt
     8881 caaagaaaca cttggagacc ttccaatcat tgcagaagac cttggaaaca ttgacgctaa
     8941 agctcgcaaa ttgcttgcgg attgtggcta tccaggcatg aaaatcttag aatttggttt
     9001 ctttgatgta acaggtaaga gtatcgatgc tccacaccgt tgcattccaa attcagttgc
     9061 ctacactgga actcacgata atgaggttgt caatggctgg tacaacaatc ttgatccaga
     9121 acaacaagaa tacgtggatg cctacagcaa ccgtaagccg attgaaaaag ttagccaagc
     9181 tatgctccgt atgctgtttg caacagtgag tgatacagct atcgcaacca tgcaagacgt
     9241 cctagacttg ggagaagaaa gtcgaatgaa tatgccgtca actatcggtg gaaactggga
     9301 atggcgcatg aaagctgaag acttgaccca agaacgcaaa gacttcttga caaaaatgac
     9361 attactatac caacgaggaa atgaaaacca tgactaaatt tacaacattt gcagaaacaa
     9421 atacttcgaa gaaattagct gatttgacaa atgaagaaat ctatcttcaa ctgttgaact
     9481 atgtaaaaga tgcagcagct tcaaaaccaa aaaacacagg aaaacgtaaa gtttactata
     9541 tctcagctga gttccttatc ggtaaactct tgtcaaacaa cttgattaac ttgggtgtgt
     9601 ataaagacgt tcaagcagaa ttggcagcag ctggtaaatc attggcgcag gttgaagatg
     9661 ttgaaccaga accatcactt ggtaacggtg gtttgggacg tctagcttca tgtttcgttg
     9721 attctatgtc aacacttgct atcaacggtg aaggtgttgg tctgaactac cactgtggtc
     9781 ttttccgcca agtatttaag aaaaatgagc aagaagcaga gccaaacttc tggattgaaa
     9841 atgattcttg gttgattcca acaactatca gctatgatgt tccattcaaa aacttcactt
     9901 tgaaatcaaa attggatcgt cttgatattc ttggctataa aaaagagact aagaactacc
     9961 tcaatttgtt tgatattgag tcagtaaact atgacttgat tacagacggt atttcatttg
    10021 acaagactga tattaaagaa aacttgacac tcttcttgta cccagatgat tctgataaga
    10081 atggtgaatt gctccgtatc taccaacaat atttcatggt atcaaatgct gctcaacttt
    10141 tgattgatga agcaattgag cgcggttcaa acttgcatga cttggctgac tacgcttatg
    10201 tgcaaatcaa cgatactcac ccatcaatgg tgattcctga gttgatccgt cttttgactg
    10261 aaaagcatgg tattgaattt gcggaagcag ttgccatcgt taagaatatg actggttaca
    10321 caaaccacac tatcttggcg gaagcacttg aaaaatggcc acttgagttc cttgaagaag
    10381 tggtgccaca cttggttgat atcatcaaag aattggatgc ccttattcgt gcagaaatta
    10441 aagacccagc agtccaaatc attgatgaat caggtcgtgt acacatggct cacatggata
    10501 tccacttctc taactcagtg aacggtgtag ctgcgcttca cacagaaatc ctcaaaaact
    10561 ctgaattgaa agctttctac gagctttatc cagaaaaatt caacaataaa acaaacggta
    10621 tcactttccg ccgttggttg gaatttgcca accaagacct tgctgattac atcaaagaat
    10681 tgattggcga tgaatacttg acagatgcga ctaaacttga aaaattgctt gcctttgctg
    10741 atgacaagga agttcatgct aagttggctg aaatcaagtt caacaataag ttggctctca
    10801 aacgttacct taaagacaac aaaggtatcg agctggatga aaattctatt attgatacac
    10861 aaatcaaacg tttccacgag tacaaacgcc aacaaatgaa tgccttgtat gttatccata
    10921 aataccttga aatcaagaat ggtaaccttc cgaaacgtaa aatcactgtt atcttcggtg
    10981 gtaaagcggc acctgcctac atcattgcac aagacatcat tcacttgatt ctttgcttgt
    11041 cagagttgat caacaatgac ccagaagtaa ataaatacct caatgttcac ttggtagaaa
    11101 actacaatgt gacagttgct gagaaactca tccctgcaac agacatttca gaacaaatct
    11161 cattggcttc taaagaagcg tcaggtactg gtaacatgaa attcatgctc aacggtgcat
    11221 tgacacttgg tacaatggat ggtgccaacg ttgagattgc tgagttggct ggtatggata
    11281 acatctatac atttggtaaa gattcagata ccatcatcga cttgtacgat aaggctggat
    11341 atgtatctgc ggactactac aatggcgatg ccaatatcaa acgtgcagtt gactttatcg
    11401 ttagcgatga ggtaaaagca cttggtaacg aagaacgtct tggccgtctg caccatgaat
    11461 tgatttcgaa agactggttc atgaccttga ttgacttggc tgaatacatt gaagtaaaag
    11521 aacaagtctt tgcagactac gaagatcaag attcatggaa caagaaagtt gttcataaca
    11581 ttgccaaagc tggattcttc tcatctgacc gtacgattga acagtacaat gaagatattt
    11641 ggcacagtaa ctaattgaat tccaacaatt agtaggaaag ttgattgaac cactccaaaa
    11701 cttatcagat caattcttcc tatgtgttga agaataaaaa ccgaccagtt tttaccatta
    11761 ttggtactct ccttagtata taaatgcaaa gaatgtctct gcaaaagcag agacattctt
    11821 tttataacta taaaattcta gaagttttgt acacttgatc aaaaaaatcg aaagtttcct
    11881 tttaccgttt cgccattgat acaaagctaa ctttgtctgg acggtaacta attgttccat
    11941 actggaaagc ttgaccattt gaaagatagg tgatgctagt gaccacgacg accatgtcat
    12001 agtctcctaa gtccatcaat tgtttttctt cttcgtttgc aaaacggaag gaaatttcac
    12061 gtctggaatg agaaattgtt aaatggagtt cttcttctaa gtagcggtaa atagagcttt
    12121 cggcaatttc tttgctgaga taagggacga ccctacggtc aaaataggaa atgtcatatt
    12181 ccaaccgctc accgtcaatt gtccgtaccc gactcactcg atagaaatct accttatcat
    12241 caacttcgaa agttgacatt aaatcttctt gaccttgaat aatataaagc gaagtaagct
    12301 gggtctgaat ttggtgctgt gatcttttgt tcaattcgcc cgctgtttta atttcagaaa
    12361 gatattggtc cttcattaga ccatgttcga taacaataga agatttcccc tgtttctttt
    12421 gaatataacc atccatttca agaagagata gtgctttgcg aatggtgtcc ttggagtagg
    12481 aatacacttc cattaattca ttttctgtcg gtagggcctg tccagttgac caaacctgct
    12541 cttcgatttt tttcttaata tcttgataaa cttttttata cttactcata gtttcctgtc
    12601 tttct
//
LOCUS       DBNFCK010000053        12488 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.053, whole genome shotgun sequence.
ACCESSION   DBNFCK010000053 DBNFCK010000000
VERSION     DBNFCK010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12488
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.053"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            13..684
                     /locus_tag="AC1PIW_001154"
     CDS             13..684
                     /locus_tag="AC1PIW_001154"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HIP6606096.1"
                     /translation="MDFNWEEFLDPYIQTVGELKIKLRGIRKQYRKANRHSPIEFVTG
                     RVKPIESIKEKMALRHIKLENLAQDMQDIAGLRIMVQFVDDIEEVLAILRKRKDMQIV
                     HERDYINNMKASGYRSYHVVIEYPVDTINGNETVLAEIQIRTLSMNFWATIEHSLNYK
                     YKGNFPEEIKKRLEVTAKIAYELDEDMRKIRNDIQEAQALFDPAYRKLNDGVGNSDDT
                     DEEYR"
     gene            668..1486
                     /locus_tag="AC1PIW_001155"
     CDS             668..1486
                     /locus_tag="AC1PIW_001155"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HIP6606097.1"
                     /translation="MKNTGRKKIALLASRNPKSEAVSKELWTKLKEANFILTPKNPDI
                     VISIGGDGMLLSAFHKYEKLIDRVRFVGIHTGHLGFYTDYRDFEVDKLIENLKLDTGA
                     RVSYPILNVKIKMTDGRIVEARALNEATVKRLSKTMVADIIINNVPFERFRGDGISVS
                     TPTGSTAYNKSLGGAVLHPTIEALQIAEVASLNNRVYRTLGSSVVVPKKDKIVIEPKH
                     SDRYSIAVDNKTFVYDSIESIEYQIDNSKIHFVATPSHTSFWNRVKDAFIGEVE"
     gene            1486..2379
                     /locus_tag="AC1PIW_001156"
     CDS             1486..2379
                     /locus_tag="AC1PIW_001156"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIP6606098.1"
                     /translation="MRFEFIADQHTKIKTFLKKHGVSKDLLAKIKYTGGNIWVNDIEQ
                     NATYLLDIGDRVTIDIPAEEDLTGSLKPIFFPLDIVYEDDHFLAINKPVGYASIPSAL
                     HSSTIANFVKGYLIEQNYENKQVHIVTRLDRDTSGVMLFAKHGYAHARLDKQLQAKLI
                     HKRYYALVKGNGQLEETGDIIAPIGRPEDSIITRCVTKNGKYAHTSYRVVQSWGDIHL
                     VDIQLHTGRTHQIRVHFSHIGFPLLGDDMYGGSLECGIERQALHCHNLAFDNPFSAER
                     IDLEAPLPEDFQAVINQLISK"
     gene            2397..3371
                     /gene="pta"
                     /locus_tag="AC1PIW_001157"
     CDS             2397..3371
                     /gene="pta"
                     /locus_tag="AC1PIW_001157"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HIP6606099.1"
                     /translation="MKIFEQLATKLEGKKVRIVLPEGEEPRILQATKRLVNESDVVPV
                     LLGNPDRIKIYLDIEGITEGFEIIDPAHYDRYDEMVAALVERRKGKVSEEQAHELLKD
                     VNYFGVMLVYMGVVEGMVSGAIHSTASTVRPALQIIKTEPGVSKTSGAFLMVKGEERY
                     IFGDCAINIDPDAQTLAEIAINSARTAKMFGIDPKVAMLSYSTKGSGSGPKVDKVVEA
                     TKIAQKLRPELDLDGELQFDAAFVPATGKLKAPGSSVAGQATVFIFPGISAGNIGYKI
                     AERMGGFAAVGPVLQGLNQPVNDLSRGCNPDDVYNLTLITAVQALEHR"
     gene            3373..5112
                     /locus_tag="AC1PIW_001158"
     CDS             3373..5112
                     /locus_tag="AC1PIW_001158"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6606100.1"
                     /translation="MKFLSRYFKDYIKESILGPVFKLLEACFELLVPLIIAYIVDTII
                     PNGSQGNLVAMLFLLVGIACIGIIVSLIAQYYSAKAAVGVTKELTNDLYQKVLSLPKS
                     SRDILSSSSLLTRLTSDTLQIQTGINTFLRLFLRAPIVVFGSLIMAFYISPSLSAYFL
                     GMIILLIFIVTVISVMTSRIYQSMRKELDGLVGQVRETVTGWRVIRAYGQREREIKAF
                     QGINQIYKKQQLQAGFWSSLLSPLTFLVVNSTLLILIWQGNIAISHNLLEQGMLVALI
                     NYLLQILVELVKMIMVVSTLNQTYISAQRIQEVFDQTSEDVESSLPKVVSEDKEIIFS
                     VRHLSFSYPKSAEESLSDITFDLRKEQFMGIIGGTGSGKSTLVDLLQALYSVPTNQLS
                     LFIDGKSPKNLKEWRQRIAIVPQQAQLFAGTIRSNLSLGLEEISDSDLWSALEIAQAK
                     SFIEDKGGLDSPVEAFGKNFSSGQRQRLTIARAILQKAPILILDDATSALDYLTESRL
                     LAAIRQELPGQNLIMVSQRTNSLRTADQILVLEQGRQVGLGRHEDLLRSSAIYQEIHQ
                     SQQQGEEDSYETI"
     gene            5099..6841
                     /locus_tag="AC1PIW_001159"
     CDS             5099..6841
                     /locus_tag="AC1PIW_001159"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6606101.1"
                     /translation="MRQSSFKELVRLVLHQPLRMSLMLVGTLVQVLLTVYLPILIGQA
                     VDAVLIANGQVLLGILGKMVMVILLNTLVQWYLPLVTNRLVYGMVADLREQVYVKLHQ
                     MPLSYLDRQSVGDLVARFSSDSEQLTNGLLMIFNQFFIGISTIFLTIFTMARLDLTMM
                     LVVVALTPVSLLVARYIAKKSYGYYRQQTQARGRQSQLLEESISQLTLVQSFNAQEQF
                     TQGFQVVNDQYATYSQQAIFASSTVNPSTRFVNAIIYALLAGLGALRIMAGNFTVGSL
                     TTFLNYASQYSKPFNDISSVLSELQSALACADRLFAILALDSIDDQAERKIDSEELKG
                     AISFDNVSFSYSPERSLIEHLDIAVKAGQKVAIVGPTGAGKSTMINLLMRFYDVTAGQ
                     IVLDGVPISQYSREDLRRQIGMVLQETWIKSGTIHDNIAYGYPNATRELVIEAAKAAN
                     ADFFIRQLPQGYDTVLADGGEGLSQGQRQLLSIARVFVKIPKILILDEATSSIDTRTE
                     ILVQEAFAKLMEGRTSFIIAHRLSTIQSADLILVMVDGKIVEQGNHEALMAEQGVYYQ
                     MQTSQQTEEDESVR"
     gene            6825..7217
                     /locus_tag="AC1PIW_001160"
     CDS             6825..7217
                     /locus_tag="AC1PIW_001160"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIP6606102.1"
                     /translation="MKVLDKLNLQPITVLDDYQATMLNEKEVLMTTKVTEKSLNPYGM
                     AHGGFLFTLADSVAGLTTVASGSYSVTLQSNIHYMKAAKLGDTLSVIGSCTHDGSRTK
                     VVEVKIENQDKQLLASASFTMFVTGKVE"
     gene            7395..8555
                     /gene="mutY"
                     /locus_tag="AC1PIW_001161"
     CDS             7395..8555
                     /gene="mutY"
                     /locus_tag="AC1PIW_001161"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HIP6606103.1"
                     /translation="MLNLKEYGIEMWPEEKIQAFRKALLDWYDANKRDLPWRRTKDPY
                     AIWVSEIMLQQTRVDTVIPYYERFLHHLPTISDLAQAPEEVILKLWEGLGYYSRVRNM
                     QKAAQQMVEDFDGQFPTTHAAISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLSR
                     LFEVDYDIGLPANRKIFQAMMELLIDSERPGDFNQALMDLGSDIESPVNPRPQDSPVK
                     AFSAAYLNGTMDKYPIKAPKKKPIPVAYQGFLIRNKDNQFLLEKNNEAGLLSGFWSFP
                     LLEKGAIVDKQVSLFEVAEEVVQPDIRQSFTELYGLTVDWQEQEFGIVQHIFSHRKWQ
                     IEMVEGVAETLNLPESKELKWVSVEDFPTYPFAKPQQKMWENFIKEKEKQKS"
     gene            complement(9218..10018)
                     /locus_tag="AC1PIW_001162"
     CDS             complement(9218..10018)
                     /locus_tag="AC1PIW_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIP6606104.1"
                     /translation="MILGDILKEYRSKNKLSMDKFAELSGLTKGYISMLEKNQHPKTK
                     KALLPTMDTLEKVAKGMAISVGELIEQLDSNQDIALTITPSQFKLFQQPIHPEVLTLD
                     NELKGDFHAQWLTFGQEQLAAMQTASVTPSASILHLDDYRERIELPVPGKVSAGTGYW
                     QDTDMNNLVSFYNDEIPDNQSYDTIAQVVGDSMAPHIEDGDYLFIRLTPHIPLNTIGI
                     FSVNGENFVKKFKGDYLQSLNSDYDDIYFSPEDDIRPIGQVVDIYSPD"
     gene            10149..10493
                     /locus_tag="AC1PIW_001163"
     CDS             10149..10493
                     /locus_tag="AC1PIW_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606105.1"
                     /translation="MKRITEISWNDIYKEWETYANHFGLATPINTEKLRNQKSKDFGK
                     GSLITLDLLADYDTDFEKTAAIWVALFCRDLIQDYAYLLNGRAYLTVNQIYFQALKQF
                     QSEAVIWSKPLT"
     gene            10639..11181
                     /locus_tag="AC1PIW_001164"
     CDS             10639..11181
                     /locus_tag="AC1PIW_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606106.1"
                     /translation="MFNLLNKKAEVSKVAEYWNDTLIERGILSANELLEGKCWRCKSS
                     HGVNMCQIVSSKWSKDTSLANQMVLCLSCQHEKPNVADTEIVWQWLEVENNERYWTLQ
                     GMAEYEKMYKKSVLQELWDMGIRDGEEVDTLVNKVTSLSRKNDIVLNRATLAGLFRCE
                     IEQMRRKAFLNWTGIFKLVS"
     gene            complement(11408..12454)
                     /locus_tag="AC1PIW_001165"
                     /pseudo
     CDS             complement(11408..12454)
                     /locus_tag="AC1PIW_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647375.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT         3730 a         2183 c         2722 g         3853 t
ORIGIN      
        1 gaaaggggcc gtatggactt taactgggaa gaatttttag acccctatat tcagacagtt
       61 ggtgaattga aaatcaaact gcgggggatt cgcaagcagt atcgcaaggc taatcggcat
      121 tctccaattg agtttgtcac aggacgggta aaaccgattg aatccattaa agaaaaaatg
      181 gctctgcgac atattaaatt ggaaaatctt gctcaagata tgcaagatat tgccggtctt
      241 cggattatgg ttcaatttgt ggacgacatt gaagaagtat tagctatttt gcggaaacgc
      301 aaggatatgc aaattgtgca tgaacgggat tatatcaata atatgaaagc ctctggctac
      361 cgttcatatc atgtggtgat tgagtatcct gtggatacta taaatggcaa cgagacggtt
      421 ctggcagaaa tccagattcg aaccctttcc atgaatttct gggctacaat tgaacattct
      481 ctgaattata aatataaggg aaattttcca gaagaaatca agaaacgctt ggaagttaca
      541 gcaaaaattg cttatgaatt agatgaggat atgcgaaaaa tccgcaacga cattcaagaa
      601 gcgcaggcct tatttgatcc tgcctatcgc aaactgaatg acggtgtggg caacagtgat
      661 gatacagatg aagaatacag gtagaaaaaa aatcgctctg ctcgctagtc gaaatcctaa
      721 gagtgaagcg gtgtccaaag aattatggac aaagttaaaa gaagcaaatt ttattttgac
      781 tcctaaaaat ccagatattg tcatttctat cggtggagat gggatgcttt tatctgcttt
      841 tcacaaatat gaaaagttga ttgatcgtgt acgttttgta ggaattcaca cgggtcattt
      901 ggggttttat acagattatc gcgattttga agtggacaag ctgattgaaa atcttaagct
      961 tgatacaggt gctagagtat cgtatccgat tttaaatgtt aagatcaaga tgacagatgg
     1021 tcgcattgtc gaggcgcgtg ccttaaatga ggcgacagta aaacgtcttt ctaagaccat
     1081 ggtcgcagat attattatta acaatgtgcc cttcgaacgc tttagagggg atggaatttc
     1141 ggtttcaacg ccgacaggct caacagccta taacaagtca ttaggtggtg ctgttttaca
     1201 tccaacaatc gaggcattgc agattgcaga agtggcaagt ttgaacaatc gtgtttaccg
     1261 caccttgggt tcatccgttg ttgtccctaa aaaagacaag attgtgattg aaccaaagca
     1321 tagtgaccgt tactcgattg cggtagacaa taagactttt gtctatgata gtattgaaag
     1381 tatagagtat cagatagaca atagtaagat tcattttgtt gcaacaccga gtcatactag
     1441 tttttggaat cgggttaagg acgcctttat tggagaggtg gagtaatgcg gtttgaattc
     1501 attgctgacc agcatactaa aataaaaact tttttgaaaa aacatggtgt ttcaaaagat
     1561 ttgttggcca aaattaagta cacaggtggc aatatttggg tcaacgatat tgagcaaaat
     1621 gcaacttact tgctagatat tggagatagg gttacaattg acatacctgc tgaagaggat
     1681 ttgactggta gtttaaaacc gattttcttt ccgttggata ttgtctatga agatgatcat
     1741 tttctggcga taaataagcc ggttggctac gcctccattc cttctgcttt gcattcatcc
     1801 acaatcgcaa attttgtaaa aggttatctt attgagcaga attatgagaa taagcaggtt
     1861 catattgtga ctcgtctgga tagggataca tcaggcgtca tgctgtttgc caagcacggc
     1921 tatgcccatg cacgactcga taagcagtta caggccaagc ttattcataa acgttattat
     1981 gctttggtga aggggaatgg gcagctagaa gagacagggg atattattgc ccctatcggt
     2041 cgtccagaag acagcatcat tacccgttgt gtgacaaaga atggaaaata tgctcatact
     2101 tcctatcgtg ttgtgcaatc gtggggagat attcatttag tagatattca actacatact
     2161 gggcggactc atcagattcg tgttcatttt tctcacattg gatttccatt gttaggagat
     2221 gatatgtatg gtggaagttt ggaatgtggg atagaacgtc aagccttgca ttgtcataat
     2281 ttggccttcg acaatccttt ttcagccgaa aggattgatt tggaagcacc gcttcctgag
     2341 gattttcagg ctgtcatcaa tcagttaata agtaaataaa tttaaggagt ttgttcatga
     2401 aaatttttga gcaattagct acaaagctag agggtaaaaa agtacgtatc gtattgccag
     2461 aaggtgaaga accacgtatc ttgcaggcga caaaacgttt ggtaaatgag tcggatgtag
     2521 tacccgtctt gcttggaaac ccagatcgta ttaagattta ccttgacatt gaagggatta
     2581 cagaaggttt tgaaattatt gacccagctc attatgatag atatgatgag atggtagctg
     2641 ctttggtaga gcgccgtaaa ggtaaggtat cagaagagca ggcacatgag ttgttgaaag
     2701 atgtgaacta ctttggtgtt atgttggtat atatgggcgt agtagaaggt atggtatctg
     2761 gtgcgattca ttcaactgct tcaacagttc gtccagctct tcaaatcatt aaaacagagc
     2821 caggtgtatc gaaaacatca ggtgccttct tgatggtaaa aggtgaagag cgttatattt
     2881 ttggtgactg tgctatcaac attgatccag atgctcaaac cttggcagaa atcgctatca
     2941 actcagcacg tactgctaaa atgtttggta ttgatccaaa agttgcaatg ctcagctatt
     3001 caacaaaagg atctggctca ggtcctaaag ttgataaggt ggttgaagca actaaaattg
     3061 ctcaaaaatt gcgtccggag ttggatttgg acggtgaatt gcaatttgat gctgcctttg
     3121 tccctgctac aggtaaatta aaagcaccag gttcgagtgt tgctggtcaa gcgactgtat
     3181 ttatcttccc aggtatctca gctgggaata ttggttataa aattgccgaa cgtatgggtg
     3241 gatttgcagc ggtaggtcct gttttacaag gtttgaatca gcctgtaaac gacttgtctc
     3301 gtggttgtaa ccctgatgat gtttacaact tgaccttgat tactgcggtt caggctttgg
     3361 agcaccgtta atatgaaatt tttatcacga tattttaagg actatattaa ggaatccata
     3421 ttaggtccag tttttaaact attggaagct tgttttgagc ttttggtacc attaattatc
     3481 gcctatatcg ttgatacaat tattccaaat ggcagccagg gaaacctggt tgctatgctt
     3541 tttttactgg ttggtatagc atgtattgga attattgttt cactaatagc gcaatattat
     3601 tcagctaaag cagcagttgg agtgactaaa gagctgacga atgacttgta tcagaaggtt
     3661 ctttcccttc ccaagtctag ccgagatatt ctttcctcat ccagtttgct gacacgattg
     3721 accagcgaca ctttacaaat tcaaacgggt attaatactt ttcttcgttt gtttttacgt
     3781 gctccaattg tcgtatttgg ttcgctcatc atggcattct atattagccc aagtctatct
     3841 gcctattttc taggaatgat tatcctattg atttttatag tgacagtcat ttctgttatg
     3901 accagccgta tttatcaatc gatgcgaaaa gaattagatg gtttggtggg gcaggtacgt
     3961 gagaccgtga ctggttggcg agtgattcgt gcatatggac aaagagagcg ggaaattaag
     4021 gcttttcaag gcataaatca gatttataag aagcaacagt tgcaggctgg tttttggtct
     4081 agtcttttat cgccattgac atttttggtg gtcaacagca ccttactcat tctcatctgg
     4141 caaggaaata tagccatttc acacaatttg ttggaacagg ggatgttggt cgctcttatc
     4201 aactaccttc ttcaaatttt agtcgaattg gtcaagatga ttatggtcgt gtctactctc
     4261 aaccagacct atatttcagc gcaacgtatt caagaggtgt ttgaccaaac atctgaggat
     4321 gtagagtcta gtttgccaaa agtggttagt gaggataagg aaatcatctt ttctgtgcgt
     4381 catctttcct tttcttatcc aaaatctgct gaggagtccc tgtctgacat tacttttgac
     4441 ttacgtaagg aacagtttat gggaattatc gggggaactg ggtcaggtaa gtccacccta
     4501 gtagatttat tgcaggcact ctattcagtg ccaacaaatc agctctctct cttcattgat
     4561 ggaaaaagtc ctaagaattt aaaagagtgg cggcagcgaa ttgccatcgt tccgcaacag
     4621 gcccagcttt ttgctggaac tattcgttct aatttatctt taggattgga agaaatatct
     4681 gatagtgacc tatggtctgc tttagaaatt gcacaggcta aatctttcat agaggataaa
     4741 ggtggtttgg atagccctgt cgaagccttt gggaagaatt tttctagtgg acagcgtcaa
     4801 cgtttgacca ttgcacgcgc tattttacag aaagcaccga ttttaatctt agatgatgca
     4861 acatctgctc tggattattt gacggagagt cgattattag ctgctattcg ccaggaatta
     4921 ccaggtcaga acttaatcat ggtgtcacaa agaaccaata gtttacggac agctgatcag
     4981 attttggtct tggaacaagg gcgtcaagtt ggacttggtc gccatgagga cttgctgaga
     5041 agctctgcta tttatcaaga aattcaccaa tcgcaacaac aaggagagga ggatagctat
     5101 gagacaatct agttttaagg aactggtcag attggttcta caccagcccc ttcgaatgtc
     5161 cttgatgttg gtgggaactt tggtgcaagt ccttctgaca gtttatctgc ctatcttgat
     5221 tggtcaggca gttgatgcag tattgatagc aaatggtcag gtcttgctcg gtatcttagg
     5281 caaaatggtg atggttatct tgcttaatac actggtgcaa tggtacttac ctttggtaac
     5341 caatcgcttg gtttacggaa tggtggccga cttgcgtgaa caggtctatg tgaagctgca
     5401 ccagatgccc ttatcttatc ttgatcgtca atcagtcggt gacttggtgg ctcgtttttc
     5461 aagtgatagc gagcaattaa caaatggtct cttgatgatt ttcaatcaat ttttcattgg
     5521 tatctcgacc attttcctaa ccattttcac tatggctagg cttgacttga cgatgatgtt
     5581 ggttgttgtt gccctgactc cagtttctct gcttgtggca cgctatatcg ctaagaaatc
     5641 ctatggttat tataggcaac agactcaggc acgtgggagg caatctcagc ttttggaaga
     5701 gtccatcagt caattgacac tggttcaatc tttcaatgct caagagcaat ttacgcaagg
     5761 ttttcaagtt gtgaatgacc agtatgcgac ctattcccaa caggctatct ttgcctcttc
     5821 aacggtcaat ccaagtactc gctttgtcaa tgctattatt tatgccttac ttgcaggact
     5881 tggagcgcta agaattatgg caggaaattt tacagtggga tccctgacaa ccttcctcaa
     5941 ttatgctagt cagtacagca agccttttaa tgatatttcc tcggtcctat cggaattgca
     6001 gagcgcctta gcttgtgcag acaggctgtt tgctatcttg gctctagata gcattgacga
     6061 ccaggctgag cggaaaattg attcagagga gttgaaggga gctatttcct ttgacaatgt
     6121 gtctttctca tatagccctg aacgttctct gatagaacat ttggacattg ctgtcaaagc
     6181 tggacaaaaa gtagccattg ttggacctac tggtgctggc aaatcaacca tgattaacct
     6241 tcttatgcgc ttctacgatg tgacggctgg tcagattgtc ttagatgggg ttccgattag
     6301 ccagtatagt cgtgaggatc ttcgcagaca gattgggatg gttttacagg agacatggat
     6361 caaatctggt accattcatg ataatattgc ctatggctat ccaaatgcca cgcgggaatt
     6421 ggtcattgag gcggctaagg cagctaatgc agacttcttt attcgtcagc ttccgcaggg
     6481 ctacgataca gttttggcag atggaggaga aggcttatcc cagggacaac gtcagctttt
     6541 gtctattgcg cgtgtctttg tcaagatacc taaaatcctg attttagatg aggcgacttc
     6601 ctctattgat acacggacag aaattttagt acaggaagca tttgccaaat tgatggaagg
     6661 acggaccagt tttattattg ctcatcgcct atcaaccatt caatcagctg acttgatttt
     6721 ggtcatggtg gatggaaaaa ttgttgaaca agggaatcat gaagcgttga tggctgaaca
     6781 aggcgtttat tatcaaatgc aaacaagtca gcagacagag gaggatgaaa gtgttagata
     6841 aattaaactt acaaccgatt acggttttag atgactatca agctacaatg ctcaatgaga
     6901 aagaggtgct aatgaccaca aaggtgacgg aaaaatctct caatccatac ggtatggcac
     6961 atggaggctt tcttttcaca ttggcagatt cagttgcagg tctgacaacg gtagcaagtg
     7021 gttcttattc tgtcacccta cagtccaata ttcattatat gaaggcagct aaacttggtg
     7081 ataccttatc agtaataggc tcttgcacac atgatggaag tagaacaaag gtcgtcgaag
     7141 ttaagataga aaaccaagat aaacagctac ttgcttcagc aagttttacc atgtttgtaa
     7201 caggaaaagt agaataaaac tcttcgaaca tcaaaagcag acagtcttgg ctagatttag
     7261 aaaaacaaat tcgttcgctc tatttccaac ctccaaaggt tccccaaacc tttggagcta
     7321 gtttgatttt gattataaac gatataaggg agttggcaaa agctggctcc ctttctttgt
     7381 gttatactag atgtatgtta aatttgaaag aatatggaat cgaaatgtgg ccggaggaaa
     7441 agattcaagc ttttcgcaaa gcactcttag attggtatga tgccaataaa cgtgacttgc
     7501 cttggagacg gaccaaggat ccgtatgcta tatgggtttc tgaaatcatg cttcaacaga
     7561 caagagtaga cacggtcatt ccttattatg aacgttttct ccatcacttg cctacgatta
     7621 gtgatttagc acaagcacca gaggaggtca ttctcaaact ctgggaggga ttgggctatt
     7681 attcgcgcgt gcggaatatg caaaaggcgg ctcagcaaat ggtagaagat tttgatgggc
     7741 aatttccgac cacgcatgca gccatttcaa gcctaaaggg cattggtcct tatactgctg
     7801 gagcaatttc cagtattgct tttaatttgc cagaaccagc ggtagatggc aatgtgatgc
     7861 gggttctcag ccgcttgttt gaggttgatt atgacattgg tcttcctgcc aatcgcaaga
     7921 ttttccaagc gatgatggag ctattgattg attcagagcg accgggcgat ttcaaccaag
     7981 ctctaatgga tctagggtcg gatattgaat cgccagttaa tccacgtccc caggacagcc
     8041 cagtcaaagc ctttagcgca gcctacttaa acggtacaat ggataagtat ccaatcaaag
     8101 caccaaagaa aaagccgatt cccgtggcct accagggatt tctcatcagg aataaagaca
     8161 atcaattttt attggaaaaa aataatgaag caggtcttct atcaggtttc tggtcttttc
     8221 cgcttttgga aaaaggagca atcgttgaca aacaagtctc tctctttgaa gttgcagaag
     8281 aagttgttca gccagatatt cggcagagct ttacggaact ttatggtctg actgttgatt
     8341 ggcaagagca agaatttggc atcgtccagc acatttttag tcaccgcaaa tggcagatag
     8401 aaatggtaga aggtgtggca gaaacactta accttccaga atcaaaagaa ttgaaatggg
     8461 tttctgtaga agattttcct acctacccat ttgctaagcc gcaacaaaaa atgtgggaaa
     8521 actttatcaa ggaaaaagaa aagcaaaaaa gttgactttg gagcacataa ttatttgaaa
     8581 gcgttttata atagtggtat aatctaattg aaaaaaggaa atagttagtt aagatgttga
     8641 acgccatctg aattgctgaa aggagttctt tagcgttcgt agaagtgtat atactattag
     8701 ccatctttct ctaataaaaa gagagggatg ctagtagggg aaaagtccgt tatccctgtt
     8761 ttatatctat ttgtgatatc cattgtcaaa tgcatagcat taactcatta tttagccttg
     8821 ctacccggtt caatcagaag taggctaaga ttattcgtgt cgtaactact atgtgttcaa
     8881 gatgttgtaa cttttgaaca taaccttaga aaatcacaaa aacctctagc gattttcatc
     8941 ataataagca atctccacac tttagacaaa tagtaagagg acaatcaaag acataagggt
     9001 agcagaataa ttatttaatc attaacttgt tgtcaaataa ctatctggta atggactttt
     9061 ccaatttgac atataaaaag gaggctgtca tagcctcctt ttactgtgct tttcaatttg
     9121 ttttcagtag gaaaaaacgc cagctatacg gacagacttt ccaaaactag atggatgaat
     9181 gtagcatata taactagtga ttaactaggc ttaaaaatca atctggacta taaatatcta
     9241 caacttgacc aataggtcga atatcgtctt ctgggctaaa atagatatca tcatagtcag
     9301 aattgagtga ttgcaaatag tcacctttga actttttaac aaaattttcc ccattgactg
     9361 aaaaaattcc aatcgtattt agaggaatat gaggagttag gcgaatgaag agatagtctc
     9421 catcctcgat atgcggagcc atagaatctc ccacaacctg agcgattgta tcataagatt
     9481 ggttatctgg aatctcatcg ttatagaatg atacaagatt gttcatatct gtatcctgcc
     9541 agtaacctgt accggcagaa accttaccag gaacaggtaa ttcgattcgc tcccgatagt
     9601 catccaagtg aagaatagaa gcagaaggag taactgaggc agtttgcata gctgctaact
     9661 gttcttgacc aaaagttaac cattgtgcat ggaaatcacc ttttagttca ttgtccaagg
     9721 ttagaacctc tggatgaata ggctgttgaa agagtttaaa ttgggaagga gtaatcgtta
     9781 aagctatatc ctgattggaa tctagttgct cgattagctc accaaccgat atagccatgc
     9841 ccttagcgac tttttctagc gtatccatcg ttggcaggag agcttttttg gttttgggat
     9901 gctggttctt ttccaacata gaaatataac cttttgttaa accagataat tcagcgaatt
     9961 tatccattga taatttattc ttgcttcggt attcttttaa aatatcacct aaaatcatga
    10021 ttgtctcctt atctgtttaa ctaattatac cattaaaaaa tattttttgc aaattttgtt
    10081 taacgtgttt aacaaaaaat caaaacggtg ttatgataat ctcacaaaaa gaaaaatagg
    10141 agtcgcaaat gaaaaggatt acagagattt cttggaatga tatttataaa gagtgggaaa
    10201 cctatgctaa tcattttggt ttggcgactc cgataaatac tgaaaaattg cgaaatcaga
    10261 aatcgaagga ttttggaaaa ggaagtctga ttacgctgga cttattagcc gattacgata
    10321 ctgattttga gaagaccgca gctatttggg tagctttatt ttgtagagat ttaatacaag
    10381 attacgcata tttactaaat ggtagagcct atcttacagt caatcagatt tattttcagg
    10441 cactcaaaca attccagtca gaagcggtaa tatggtcaaa acctctcaca tgattgcaac
    10501 cgaagttatt tgtatcttat cgtttgttgg aaaatcttga tttgagtcat tattcttgtg
    10561 tagttgagtt agcaatgttg taggctagta tggttcggac acagatatta gaaaagtaat
    10621 tatagtagga gaatgaaaat gtttaatttg ttgaataaga aagctgaagt atcgaaagtt
    10681 gcagagtatt ggaatgatac cttgatagaa agaggaattt tgagcgctaa tgaattgctc
    10741 gaaggaaaat gctggagatg taaaagttct catggtgtaa atatgtgtca gattgtatca
    10801 agcaaatgga gcaaggatac gagcctggct aatcagatgg tcctatgtct atcctgtcag
    10861 catgaaaaac ctaatgtagc tgatacagaa attgtgtggc aatggttaga agttgagaat
    10921 aatgaacgtt actggactct acaaggtatg gcagaatatg agaaaatgta taaaaaatca
    10981 gtacttcaag agttatggga tatgggaatt cgagatggtg aagaagtcga tacgcttgta
    11041 aataaagtaa ctagcctttc tcgtaagaat gatattgttt taaatagagc aaccttagca
    11101 ggattatttc gctgtgaaat cgaacaaatg cgtcgaaagg catttctaaa ttggacagga
    11161 atttttaagc tagtcagtta accgatacag ttgtaggaaa acatctcatg ctgattggag
    11221 taacagataa ataaaaagcg catcagctta gaatcttaaa gaaagaagtc taatgatgcg
    11281 tttatagggt tatatttcag ctaataaaat tggagtgaat gtttttgaat tatagctcaa
    11341 aacgttaatc caaacgttat ggcgacaaca tttgtgccga ctttttggtt caaattggtt
    11401 caaatgttta ctcgcttaat gaattcaata aatttaaggc ctcagcatct tgctggtgtc
    11461 ttttttctgg catcaattct agataatact gttgtgttgt taagatagag ttatgaccta
    11521 gacgacgaga aatatagtct aggctaatat cttttgaaaa gagaaatgag gcatgcgtgt
    11581 cacgaagtcc atgaaatgtc gtttttgtta atcccaactg ttttaacagc ttttgaagtt
    11641 gactagcttg cttgaatcca tttcaatcaa agatatagcc ttcttttatt tgtgcaagct
    11701 ttgtcacagc ttgataaaca tcttcgttga tggaaatatc acgcttagaa tatttggttt
    11761 taagctgggc atcgtcatta gttggactaa ttgatcgaag aattttgata ccatattcaa
    11821 aaatatcttc ttttttaatt cctaaaagtt ctccgcgcct tgctcctgtc tcaagggcga
    11881 gagcaacaag aacattaaat tcatcctcag tatgacttag gcagtattgt ctgagttttt
    11941 taaattctgt gatagatagt ggtatatttc gtttaggttg ttcttgtcct tttgatttca
    12001 aaagatgacc aaaatcatta ctaatcaaac cacgagcata cgcatatttt aatgaaccat
    12061 ggattttaag aacaagttct tttactgttt ttttagagtg ggtttcagcg tattggtcga
    12121 ttttcttttg catgacgaga tcgtcaaggt gtttaagttg aataccatca aaaaggtcat
    12181 caactattac gagagttcgt ttatagttaa taaaggtggc ttcgcgaacg tcagttttct
    12241 tgaccgtatg gacccaattt ctataaaaat caggaaatag ggtggagcgt tcggcaatgt
    12301 ttttgccacg acctttttca agttccattt ctaatgtcca aagctcagca tcttttctgt
    12361 tgctaaaggt tttagtttct cgtttatatt ctcctttctt ataaagtgat acagttgcac
    12421 ggtaagagtt accacgtttt gttattgttg acattttatc agcctttcta taaaaaatga
    12481 ttgataaa
//
LOCUS       DBNFCK010000054        12398 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.054, whole genome shotgun sequence.
ACCESSION   DBNFCK010000054 DBNFCK010000000
VERSION     DBNFCK010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12398
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.054"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            50..310
                     /locus_tag="AC1PIW_001166"
     CDS             50..310
                     /locus_tag="AC1PIW_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HIP6606107.1"
                     /translation="MDVKNLRKLKRIELYEIMLAQSEEIDCLRNQLEKIKTELADRRI
                     MISQSGSIAEASMKLTNIFEEAQKAADLYLYNVKNKIGDVHE"
     gene            303..893
                     /gene="lepB"
                     /locus_tag="AC1PIW_001167"
     CDS             303..893
                     /gene="lepB"
                     /locus_tag="AC1PIW_001167"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIP6606108.1"
                     /translation="MNSSNLKGKELPKTSELKELYQHERYRHTFWRTVKSTVFMLVVV
                     AAFAILIAVLFLPILRIYGDSMKGTLNSGDIVVSVKSNDFESSDVVAFYYNNNILVKR
                     VIAEAGDWVDMDKQGNVYVNNQRLDEPYLANRDYGHTDIEFPYQVPENRIFVMGDNRK
                     ESIDSRNNAIGTVSNEQIVGKLVFKIWPLSELGWIE"
     gene            925..1074
                     /locus_tag="AC1PIW_001168"
     CDS             925..1074
                     /locus_tag="AC1PIW_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HIP6606109.1"
                     /translation="MRDWLNLEKIQGWRRKYKKLVNIIAFLVVFATTYALILPAITLD
                     QEKAE"
     gene            1090..5901
                     /locus_tag="AC1PIW_001169"
     CDS             1090..5901
                     /locus_tag="AC1PIW_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HIP6606110.1"
                     /translation="MQETPVTTEISKLTEPMVTTQQTPIVGSSADEVQSPATETVPDP
                     AEQVSTESASKPQTDQERITEPTTIVHKTNDYEVTATFDASAQFPKDVEFNVRELDSQ
                     SEEYRTHYEKTKEKLGVKKLVYARFFDIHFSSQGQEVEPASPVKITIRNKELVQRPDD
                     SKLKIVHFEENSQIEVLEAETKENEQQISEISFDASHFSVYGDVLANYYTVKFTYTDT
                     AGQEQVITSLIDKTVGTSLGSLPEAPFKNGYVFSHWINKDTGDRIDENTVITGDLTAV
                     AVFNNINIYTIDVTYFYHNVSKNQDITIDKETVQLEASDAPYQLTPPASTEVSNKEDT
                     TLPADAIYYPEKPLLEFTADQLERLDVADGVDDNYISERVQYVPYNAEYDVIYRLKNL
                     TGSGYSDIQTVHVRGVLGSTVTPQILTYNYADFERADTAKIENTSGQKLYVYYTRKTF
                     TLSYNSNGGSNIPQQQGLYDSQIKISTTTPTKEGYTFEGWYDNPGLTGNKITGSLTLK
                     KDTTLYAKWTPNRVRYIIGYYKEVYDNNTGTTSYVYDSSAAGWGLVGTTVKGTDSNIG
                     PPPPTQTTIGSIGYEKDVTKSAASSIEIAADGTSVLKVYYKLKRYTFEFFLNDNNANI
                     TINGKTYSDRNRYMIRDVVLGQDVSKLWPASITNPKEVYDDKVYYDSYGRPIRYFFDY
                     WIFGNLLFKTDRFEVVESMLPTSGTTSKFTAYWNTQGTQANVEYWLQQPDGSYKKSDI
                     YSQSFLKTAGLLPKEIFGYSYYTDASFTEAGIRYYGTQGNTYRFYYKRQAFKIDYYYN
                     STGLDSKSNILFESNINTATYNYQPPRSTGVDADYTWGGWYTDSLLTEKYNFNKMPAN
                     NLVLYAKWIAPKFKVTFDINGGNGTAPVDQSVEKYKYAKAPADPTREHYNFLGWSTED
                     GKLYSWSDQVTKDVKLTAKWELKPLTYTVRYLEAGTNTQLAADKVITSPALVLNQAIT
                     EKALGITGYRPDKSSQTINLNFRGNEITFYYTPKVKDVVYTIKYVLKDNPAIEVAPTV
                     TRKVDGSIIRAKEAAADVNKQHLQSQSGVTAEMLEMDYYPTVNTQSLILTSSDANNVI
                     VFEYVGFDTQTFTVNYLDMDGKKIDGQEPLVVYRKKPGSFVVDHKTINGYTYDHSLDS
                     ENAADKTVYRVSKGGHITIDLYYKKNLTLTALDKSKVYDGTALSSSGLSDLNPSYSSE
                     LKTGDKLVAISYDGSQTNAGSSATTPKLANISNASGADRTNFYHITYQPGNLRVDQQP
                     VVVVIDGEKKSKIYDGKAEAVGYKVTEIRDSSGLYKKTDIKFNGSSSEQSVKKKDAGI
                     YPLLLSNRFSNTNQNFQVDFLISDGELRIDRRQLTITSDSASKAYDGSELRADNITVS
                     VPAGVDYTGFVDGEGIIPTFLGGPIDSGMMPNFFEYALKSGTNLANYELDIQFGQLKV
                     KEVIKIQKTDLAWQALSGGKFELTKWDGLNWAQVDGVQEFAITSKDGIRIPVGLEAGR
                     YRLQELAAPDGFIVLDSYIYFSIKEIFNEDKTSSFYTVSLSDEAGKDASPERATLTKV
                     SGDASHRIQVANEQGRALPSTGGDGQKWFILSGLVLIAISYLMHLFVQRRR"
     gene            6008..7450
                     /locus_tag="AC1PIW_001170"
     CDS             6008..7450
                     /locus_tag="AC1PIW_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HIP6606111.1"
                     /translation="MKKLQQFFTSLVAVLTVFAFGSTALAQTVDSGKSGAGTITVSNA
                     SQGQTYTAYKLFDATVTTDGSGISYKLPAGKIATNFGGDTWFEVDSKGNITAKEGATV
                     SSEAFAAWAKNFGTEVTSATANDNSLVFTHLTFGYYFVTSSLGAVLTVDSTTPNATVI
                     DKNTTNPTIPDSNNGGGKKILSNGATTSETTAKIGDTINFQIKFNATNYVTKDRQTKQ
                     IVSYTIEDSPTALAIDQNSVNVKVDGVDITAKISKTFDATGKMNLVITWADAAASNKT
                     IYNSPAEVIITYSAVVTKDAKEGEATNSATIGYNTIDNPTTPPTPVDPDKPTETTKVT
                     THRFTLKKTNNVGETLTGAEFKLYDAANNGTEIKVVKESDGVYRVAQADEQGVVIEAG
                     EVVIKGLKHSTTYYLEEMKAPNGYNILTERQSIEVKENNTAQANIVNKKGGVLPSTGA
                     IGTTLFYLVGSILLLVALVYTISKRRMNNI"
     gene            7564..8433
                     /locus_tag="AC1PIW_001171"
     CDS             7564..8433
                     /locus_tag="AC1PIW_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HIP6606112.1"
                     /translation="MIKWLKKKGPSFIMPLIFILGIGLLAYPSVSDYWNSFHQSEAIM
                     SYSDTVSDMTADQYKEIIDSARQYNQEKPLNWNVTASDIEAYNQELNFNKDGIMGYIE
                     IPKIDVKLSIFHGTDETVLETSIGHLEGTSLPVGGLGTHSVLSGHRGLPSARLFSDLD
                     KLREGDIFTIHVLNETLTYKVDQIRVVEPSDLSALTMEPQKDLLTLVTCTPYGINTHR
                     LLVRGYRIENVNGSALVTSDAIQIKAIFIAPFIATPILFVILIYIFITTSKTFRSRNR
                     NQVLDDFLVPKKF"
     gene            complement(8547..8834)
                     /locus_tag="AC1PIW_001172"
                     /pseudo
     CDS             complement(8547..8834)
                     /locus_tag="AC1PIW_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018958.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
     gene            9005..10354
                     /gene="murD"
                     /locus_tag="AC1PIW_001173"
     CDS             9005..10354
                     /gene="murD"
                     /locus_tag="AC1PIW_001173"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIP6606113.1"
                     /translation="MKMIDIVKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     ENPTAQSLLEDGIRVICGSHPLELLDEDFALMVKNPGIRYDNPMVEKAIGKGIPVWTE
                     VELAYLVSDVPIVGITGSNGKTTTTTMIAEVLNAGKKPAKLCGNIGYPASSVAQTATA
                     EDTLVMELSSFQLMGTESFHPHIAVVTNLIASHIDYHGTFEDYVAAKWMIQRQMTAED
                     FVVINFNQKEAKELAGQTKATVVPFSTQEVVDGAYLADGKLYFKGEYIMDADQIGVPG
                     SHNVENALATIVVAKLLGVDQQAIQESLSAFGGVKHRLQFVGEINGVSFYNDSKSTNI
                     LATQKALSGFDNSKVILIAGGLDRGNEFDELVPDLVGLKKMVILGQSAPRVQRAADKA
                     EVETIEALDIADATRKAFAIAEKGDIVLLSPANASWDMYANFEVRGDVFLQTFEELK"
     gene            10357..11421
                     /locus_tag="AC1PIW_001174"
     CDS             10357..11421
                     /locus_tag="AC1PIW_001174"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HIP6606114.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLIPRFLEEGWEVHYIGDGNGIEHE
                     QVVKSGLDVHFHSIATGKLRRYFSFQNMLDVFKVGFGVLQSLTIIAKIRPQALFSKGG
                     FVSVPPVIAANLLRVPVFIHESDLTMGLANKIAYKFATTMYSTFEQPASLTKVKHVGA
                     VTKVGQTNDKVTPIQLPEILSHFDKSLPTLLFVGGSGGAKVFNDLISQNSAALTERFN
                     IINLTGDSSLNKLDKNLYRVDYVTELYQPLMDLADVVITRGGSNTLFELIAMQQLHLI
                     VPLGRQASRGDQIENALYAEKKGYSKQIDESQLTFASLLVEVDELLKHKEFYVQNMAN
                     SNEIQSVDSFYNLLREDMGR"
     gene            11427..>12398
                     /locus_tag="AC1PIW_001175"
     CDS             11427..>12398
                     /locus_tag="AC1PIW_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIP6606115.1"
                     /translation="MTEKDSNVEESVLEVEQASQVELDSEQISPAEKESVLAEEKEFS
                     TDVDIPEMTAPDDEKSAFFEQWKARHQAYLAHKDEADIQAVDEGQTEQEIPEAKKSKR
                     VLFQGIERKTESLKSKKETGEKVQTLKVDIPSKVVWKAIPVLVTSLLLAALALYFISP
                     TSKKKQIEVVGNERLTAEQVENYSLISPDDYNVTIALHADAYAKNIKKNSSSVETATI
                     KFQFPATFTIHIKEYAIIGYIQQQSQWYPVLSSGEIGGEPISQDSLPEDYTTINLSDK
                     ELIKELAIELGKIDAGIRSAIQTINLTPSKVTADLLTFNMRDGNTVLV"
BASE COUNT         3984 a         2221 c         2705 g         3488 t
ORIGIN      
        1 gtactttaac ttagttgtat aatattttgg taaaagagag gaaacgcaga tggatgtaaa
       61 aaatcttcgg aaattaaagc gaattgagtt atatgagatt atgttggctc aaagtgaaga
      121 gattgattgc ttgagaaatc aacttgagaa gattaaaaca gagttggctg atagaagaat
      181 tatgattagt caatctggct ccatagcgga agcttcaatg aaactgacta atatttttga
      241 agaagcgcaa aaagctgcgg atctatatct ctataatgtg aaaaacaaaa ttggtgatgt
      301 tcatgaatag ttcaaatctc aagggaaagg aacttccaaa aacttcagaa ttaaaagagc
      361 tatatcagca tgaaagatat agacatacat tttggaggac agtgaaaagt acagtgttta
      421 tgttggtagt agttgcagca tttgcaatac taatcgctgt attatttttg cctattttga
      481 gaatatacgg agattcaatg aaaggaaccc tgaatagtgg ggacatcgtt gtatcagtaa
      541 agagcaatga ttttgaaagc agtgatgttg ttgcttttta ttacaataac aatattctgg
      601 tcaagcgcgt catcgctgaa gctggggatt gggtggatat ggataaacaa ggaaatgttt
      661 atgtcaataa tcaaagattg gacgagccat atttggcaaa tagggattat ggtcatacag
      721 atattgagtt tccctatcag gtaccggaaa atcgaatctt tgtcatggga gataaccgga
      781 aagagtccat tgattcgcga aacaacgcta ttggtacagt ctctaatgaa cagattgttg
      841 gaaagttggt atttaagatt tggccactgt ctgaattggg atggattgaa taaatgaaag
      901 tgaaaaaata gaaaggagga tcttgtgaga gactggttaa atttagaaaa aatccaaggt
      961 tggcgacgaa agtacaagaa gctcgtgaat atcattgctt tcctcgtagt atttgcaacg
     1021 acctatgctt tgattctacc cgctatcaca ttagaccaag aaaaggctga ataaactgct
     1081 gggattgaag tgcaagaaac accagtaaca acggaaatta gtaaattaac agaaccgatg
     1141 gtgacaactc agcaaactcc gatagtgggt agctctgctg atgaagttca atctcctgcg
     1201 acagaaacgg ttcctgaccc tgctgagcaa gtgtcaacag agtctgctag caaaccccag
     1261 accgaccaag aacggattac tgagccaact accatagttc acaaaacgaa tgattatgag
     1321 gtgactgcaa catttgatgc gagtgctcaa tttcccaaag atgttgagtt taatgttaga
     1381 gaactagata gccaatcaga agaatatcgg acacattatg aaaaaaccaa ggagaagctt
     1441 ggagtaaaaa aacttgtata tgcccgattc tttgatattc atttttcatc acagggccaa
     1501 gaggtcgagc ctgcctcccc tgtgaagatt acgattagaa acaaggaatt agttcaacgg
     1561 ccagatgata gtaaactgaa aattgtccac tttgaagaga atagccagat agaagtttta
     1621 gaggccgaaa cgaaggaaaa tgaacaacaa atctccgaga tcagttttga cgctagccat
     1681 ttttctgttt atggagatgt tttagcaaac tattatactg tcaaatttac ctacactgac
     1741 acagcaggtc aagaacaagt cattacgagt ttgattgata agactgtggg gacttcactt
     1801 ggtagtttgc ctgaagctcc ttttaaaaac ggttatgtat tttctcattg gattaataaa
     1861 gatactggtg atcggattga tgaaaatacg gtgataactg gcgacctgac tgctgtagca
     1921 gtatttaata atatcaatat ctacaccatt gatgtcacgt atttctacca caatgtttct
     1981 aaaaaccaag atattacgat tgataaggaa acggtgcaac tggaagcaag tgatgctcca
     2041 tatcaactga caccacctgc ttctactgag gtgagcaaca aagaggatac aactttacca
     2101 gcagatgcta tttactaccc tgaaaaacca cttcttgagt ttacagcaga tcaactagag
     2161 cgactggacg tagcagatgg agtggatgat aattatatta gcgaaagagt acaatatgtt
     2221 ccttacaatg cagagtatga tgtcatttat aggctaaaga atttgacggg gtcaggttat
     2281 tctgatatcc agactgttca tgtccgtggg gtactgggtt caactgttac accgcaaatt
     2341 ctaacctaca actatgctga ctttgaacgt gctgatacag caaagattga gaatactagc
     2401 ggccaaaaat tgtatgttta ttatacacgg aaaaccttta ctctttctta taattccaat
     2461 ggcggtagta atattcccca acaacagggt ctatacgata gccagattaa gatttctaca
     2521 actactccaa caaaagaggg gtatacattt gaaggttggt atgataatcc tggactgact
     2581 ggaaacaaga ttacaggctc tctcacctta aagaaagata caactttgta tgcgaaatgg
     2641 acaccgaaca gggttcgcta catcattggg tattacaagg aagtctatga caacaacact
     2701 ggaacaacct cctatgtata tgatagttcg gcagctggtt ggggtctggt tgggactact
     2761 gtaaaaggaa cagattcaaa tattggacct cctccgccaa cccaaacaac cattggcagc
     2821 attggctacg agaaggatgt tactaaaagt gctgcttcaa gtattgaaat tgcagctgat
     2881 ggaacatctg ttttgaaagt ttactataaa ctgaagcgct acacttttga atttttctta
     2941 aatgataaca atgccaatat caccataaat ggtaagacct actctgatag gaataggtac
     3001 atgatacgtg atgttgtgct tggtcaagat gtctcaaagt tatggccagc ttctataaca
     3061 aatcctaaag aagtatacga tgataaagtc tactatgata gctatggtag gccgataaga
     3121 tacttttttg actactggat ttttgggaat ctactcttca aaacagatcg gttcgaagtt
     3181 gttgagtcta tgttgccaac gagtggaaca accagtaagt tcacagcata ttggaatacc
     3241 caaggtacac aggctaacgt tgaatattgg ttacaacaac cggatggttc ttataagaag
     3301 tctgatatat atagtcaatc tttcctaaag actgcaggac tgctaccgaa agaaattttt
     3361 ggatattctt attatacaga tgctagtttt actgaagcag gcattaggta ttacggaact
     3421 caaggaaata catatcgttt ctactataaa cgccaagcct tcaaaattga ctattattac
     3481 aatagtactg ggttagattc aaagagtaac attctctttg agagtaacat caatacagca
     3541 acttataatt accaaccacc acgttctact ggagttgatg ctgattatac atggggtgga
     3601 tggtatacag attcgttatt gacagagaag tataacttta ataagatgcc tgccaataac
     3661 ttggttctct atgctaagtg gattgctcca aaatttaagg ttacttttga tatcaacggt
     3721 ggtaatggaa ctgcccctgt cgatcaaagt gttgaaaaat acaaatacgc taaggcccct
     3781 gctgatccaa cgagggaaca ttacaatttc cttggttggt ctacagaaga tgggaaactc
     3841 tatagttgga gcgaccaagt aaccaaggat gtcaaattga ctgcgaagtg ggaattgaaa
     3901 ccattaactt atactgttcg ctatcttgaa gcaggtacaa atacgcaatt ggctgctgat
     3961 aaagtgatta cgagtccagc gcttgtattg aatcaagcta tcacagaaaa ggcattggga
     4021 attacaggtt atcgaccaga taagagtagt caaacaatta acctgaattt tagagggaac
     4081 gaaataactt tctactatac accgaaggta aaagatgttg tttacaccat caagtatgtc
     4141 ttgaaggata acccagctat tgaggtagca cctacagtta ctcgtaaggt tgatggaagt
     4201 atcatccgtg cgaaagaagc tgctgcagat gtgaataaac agcatttgca gagtcagtct
     4261 ggtgttactg ctgaaatgct ggaaatggat tactatccaa ctgtaaatac tcaaagtttg
     4321 attctaactt ctagtgatgc aaataatgtg attgtttttg aatatgtcgg attcgatact
     4381 caaaccttta cggtaaatta tttggatatg gatggtaaga aaatcgatgg tcaagaacca
     4441 ttggtagttt accgaaagaa accaggtagt ttcgtggtag atcataagac tataaatggt
     4501 tatacctatg atcattcgtt agacagtgag aatgcagctg ataagacagt ataccgtgta
     4561 agtaagggtg gtcacataac cattgatctc tattataaga aaaacctaac actgaccgca
     4621 ttagataagt ccaaagttta tgatggcact gccctatcaa gttcgggtct gtctgattta
     4681 aatccttcgt acagttcaga actgaaaaca ggtgacaaat tggttgccat cagctatgat
     4741 ggaagtcaaa caaatgctgg tagtagcgca acgacaccta aacttgctaa catcagcaat
     4801 gcatctggtg ctgaccggac gaatttctat cacataacct atcaaccggg taacttaaga
     4861 gttgaccaac agccagttgt ggttgtcatc gacggtgaaa agaaatcgaa aatctatgat
     4921 ggcaaagcag aagcagttgg ttataaggtg acggaaataa gagattcaag cggtctctat
     4981 aaaaaaacag atatcaaatt taatggttca agttcagagc agtcggttaa gaaaaaagat
     5041 gcaggaattt atccactcct tttaagtaat agattctcaa ataccaatca gaatttccaa
     5101 gtagatttct tgatttctga tggggaatta agaatagata gacggcaact gaccataaca
     5161 tctgactccg ctagcaaggc ttatgacggt agcgagcttc gtgctgacaa tatcacagtc
     5221 tctgtacctg ctggtgttga ctacacaggt tttgtcgatg gcgaagggat tataccaacc
     5281 ttcttaggcg gtccaatcga ttctggaatg atgccaaact tctttgaata tgctctaaaa
     5341 tctggcacga atttagctaa ttatgagtta gatatccagt ttggccagct caaggtcaaa
     5401 gaagtcatca agattcagaa aacagacctg gcctggcagg ccctttcagg tggtaagttt
     5461 gaattgacca agtgggatgg tttgaactgg gctcaggtag atggggtgca ggaatttgcc
     5521 attaccagca aagatggtat tcggataccg gtaggcttag aagcaggtcg ctaccgtctg
     5581 caagaattgg cagcacctga tggttttatc gtcttggata gctatatcta cttcagcatc
     5641 aaggaaatct ttaacgaaga taagacatct tccttctaca cagtttcctt gtctgatgaa
     5701 gcaggcaagg atgctagtcc ggaacgagca acgcttacca aggttagtgg tgatgccagt
     5761 catcgcatcc aagtggccaa tgagcagggc agagccttgc caagcacagg cggagatgga
     5821 cagaagtggt ttattctttc tggccttgtc ctcatagcca tctcctatct gatgcatctg
     5881 tttgttcaac gtcgaaggta aaggagacag taacaatctc caaatccctt agtagtaaat
     5941 cgtctcagct ttctaagtaa cgtccagttt cttagaccta atccaaataa ataaaagaag
     6001 gaatattatg aaaaaattac aacaattctt tacatccctt gtggcagtac tgacagtctt
     6061 cgcttttggt tccacggcct tggcgcaaac tgttgatagc ggaaaaagtg gagcaggcac
     6121 tattactgtc tctaacgctt cacaaggtca aacttacact gcctataaac tctttgatgc
     6181 aactgtaacc acagatggtt ctggtatctc atacaaactt cctgctggta aaattgcaac
     6241 gaactttggt ggtgatactt ggttcgaagt ggacagcaaa ggtaatatta ctgcaaagga
     6301 aggtgctact gtctcttcag aagcatttgc ggcttgggca aagaatttcg gtacagaagt
     6361 tacaagtgct acagcgaatg ataactctct tgtgtttaca catttgacat ttggttacta
     6421 ttttgtaaca tcatcacttg gtgcagtgct tacagtagat agtacaacac caaatgcaac
     6481 tgttatcgat aaaaacacta ctaatccaac cattccagat tcaaataatg gtggcggtaa
     6541 gaaaattctt agcaatggtg ctacaactag tgaaacaact gcaaaaattg gcgataccat
     6601 caacttccaa atcaagttta atgctaccaa ctacgttact aaagatagac aaaccaagca
     6661 gattgtttct tacactatcg aagatagccc aacagccctc gcaattgatc aaaattctgt
     6721 caacgttaaa gtggatggtg tggatatcac agcaaaaatt tcgaagactt ttgatgcaac
     6781 aggcaaaatg aatcttgtca ttacatgggc ggatgcggca gctagtaata aaaccatcta
     6841 taactctcca gctgaagtta ttatcaccta ctcagcagtg gtgactaaag atgccaagga
     6901 gggagaagca accaactcag cgactattgg ttacaacaca attgataatc caacaactcc
     6961 tccaacacca gttgatccag acaagccaac tgaaacaaca aaagtgacta ctcaccgttt
     7021 cactttgaaa aaaacaaaca atgtaggtga aactttgaca ggtgcagagt tcaaactcta
     7081 tgatgcagcc aacaatggaa ctgaaatcaa ggttgtcaag gaatctgacg gagtgtatcg
     7141 tgttgcgcag gctgatgagc aaggtgttgt catcgaagct ggcgaagttg tgattaaagg
     7201 tctgaaacat agcacgacct attatcttga agaaatgaaa gcaccgaatg gttacaatat
     7261 cttgacagaa cgtcaatcca ttgaagtgaa agagaataac acagcacaag caaatatcgt
     7321 caataaaaag ggtggtgtct tgccaagcac tggtgcgatt ggaacaactt tattctatct
     7381 agttggttca atcctacttt tggtagcatt ggtttatact atttcaaaac gtcgtatgaa
     7441 caatatctaa ttgtccatac aatacctaat atttagaatc tatcttcgga agtgcaaaag
     7501 caagagtgga agtatttccg aagatatttc tttggtctta acacaaggaa aggaggggat
     7561 ttcatgatca aatggctcaa aaagaaaggt ccgtctttta tcatgccatt aatttttata
     7621 ttggggattg gactattggc ttatccatcg gtcagtgatt actggaattc cttccaccaa
     7681 tcagaggcca tcatgtccta ttctgacaca gtatcagata tgacagctga ccaatacaaa
     7741 gaaatcattg atagtgcgcg ccagtataat caagaaaaac ccttaaactg gaatgtgact
     7801 gcatccgaca ttgaagccta caatcaggaa ttgaatttca ataaagatgg catcatgggc
     7861 tatattgaga ttcctaaaat tgatgtcaag ttatctatat ttcatgggac agacgaaacg
     7921 gttttagaga cttctattgg acatctagaa ggaacatcgt tgcctgtagg aggtttggga
     7981 actcatagtg tactttcagg gcatcgtggg ctcccatctg cccgtctgtt ttctgattta
     8041 gataagttga gagaaggcga tatatttacc attcacgttc tcaatgaaac actaacttac
     8101 aaagtggacc agattcgagt ggttgagccg tcagatttat cggctcttac catggaacct
     8161 cagaaagact tattgacttt ggtaacctgt acaccctacg gtattaatac acataggcta
     8221 ttagttcgtg gctatcgtat tgaaaatgtt aatggtagtg ctttggttac ctctgatgct
     8281 atccaaatta aagcaatttt tattgctcca tttatcgcaa ctccaattct atttgtaatc
     8341 ttaatttata tttttataac aactagtaaa acatttcgtt ctcggaatag gaatcaagtg
     8401 ttggatgatt ttttagttcc caagaaattt tgaactattg aaaatgtttc aaagtcgatt
     8461 tgactctgga acatttttct attatagaca ttagacaata tggctgaatg aggtaattgt
     8521 tcaacttcta acctcctgtc aactcaaatc aattgcctag accgacgatt gaaacgactt
     8581 ctttctagta actgaccgca agggaagagg tggcagatgg tgtagaaatg gcgctgtgat
     8641 gtcataccta gttcagcttg taacaaaagt aagacaagga ttgattcatc tgaaacttta
     8701 actaagccaa catttcgccg atatttgaag gaatccgggc aatagttgtt gtagaagtga
     8761 tgacaaattt gtgataattg gcgtacattc cattgcaaat gatgagattt agcagtatac
     8821 tgtaagtagc tcatttggac tacccctctg tatgtttcgt cgcttacagt atagtccatt
     8881 tgggcttttt attgtatttt gaaatgaact agcgctacgg gcagagttta cttttctgtc
     8941 ctctggcttg aaaaaggtct tcgtaaaggg taaaataagg tgttagaaag taaaggtatt
     9001 tgagatgaag atgattgata ttgtaaaaaa taagaaagtg ttagtgctag gtttagctaa
     9061 atcaggggaa tctgcagccc gtctactaga taaattgggt gctatcgtca ctgttaacga
     9121 tggaaagcct tttgaagaaa atccgactgc tcaatctttg ttggaagatg gtatccgtgt
     9181 tatttgcggg agccatccct tggaattatt ggatgaagat ttcgctttga tggtaaaaaa
     9241 tccaggaatt cgctatgata atcctatggt tgaaaaagca attggcaaag gaattccagt
     9301 atggacagag gttgaattgg cctacttggt atcagatgtt ccaatcgtgg ggataacagg
     9361 ttcgaatggc aagacaacga caacgaccat gattgcagaa gtcttgaatg caggaaaaaa
     9421 acctgcaaaa ttatgtggaa atattggcta tccagcatct tcggtcgctc aaactgcgac
     9481 tgcagaggat accttggtga tggaattatc gtctttccag ttaatgggga cagaatcttt
     9541 ccatccgcat atcgccgttg tgaccaacct cattgccagt catattgatt atcacgggac
     9601 ttttgaagac tacgtagcag ctaaatggat gattcagcgt cagatgacgg ctgaggattt
     9661 tgtggtgatc aacttcaacc agaaagaggc taaggaattg gctggacaga ccaaggcaac
     9721 ggttgttcca ttttcaacac aggaagtagt tgatggagct tatttggcag atggaaaact
     9781 ctatttcaag ggtgaataca tcatggatgc tgaccaaatt ggtgttccag gttcacacaa
     9841 tgttgaaaat gccttggcaa caattgtggt tgcaaaattg ttgggagtag atcagcaagc
     9901 gattcaggaa agtttgtctg cctttggagg tgtcaaacac cgcttgcaat ttgtaggtga
     9961 aatcaacggg gtttccttct acaatgatag taagtctacc aatatcttgg cgactcaaaa
    10021 agctctttca gggtttgaca atagcaaggt cattttgatt gcagggggct tggaccgtgg
    10081 caatgaattt gatgaattag tacctgattt ggtcggacta aagaaaatgg tgatccttgg
    10141 tcaatcagct ccccgtgttc aacgtgctgc agataaggca gaagtggaaa cgatagaagc
    10201 tctcgatatt gcggatgcga ctagaaaagc ctttgccatt gcggaaaaag gagatattgt
    10261 ccttttaagt ccagccaatg caagctggga tatgtatgcc aattttgaag tgcgtggcga
    10321 cgtcttctta cagacctttg aggagttgaa ataatcatga aaaaaattgt ttttacaggc
    10381 ggagggactg ttgggcacgt gacgctcaat ctccttttga ttccgcgatt tttggaagaa
    10441 ggctgggaag tccactatat tggtgatggg aacggtattg agcatgagca ggttgtgaaa
    10501 tcaggtttag atgttcattt ccattcgatt gcgaccggga aattgcgccg ttatttctct
    10561 tttcaaaata tgttggacgt gtttaaagtt ggttttggtg ttcttcaatc cttgaccatt
    10621 attgctaaaa ttcgtccgca agctcttttt tcaaaaggtg ggtttgtatc ggttccgcca
    10681 gttattgctg ctaatttgct gcgggttccg gtctttatcc atgaatcaga cttaacaatg
    10741 ggactggcca ataaaatagc ctataaattt gcgactacca tgtatagtac ttttgagcaa
    10801 cctgccagct tgactaaagt aaaacatgtt ggtgcggtta caaaagtggg gcaaacgaat
    10861 gacaaagtta caccaattca gttgccagaa attctcagtc acttcgataa aagtctgccg
    10921 accctactgt ttgtaggtgg ttcgggcggt gctaaggtct ttaatgattt aatcagtcag
    10981 aatagtgctg ccctgaccga acgattcaat attatcaact tgacggggga cagtagtctc
    11041 aacaaattag ataaaaacct atatagagtt gactatgtaa cagaacttta tcaaccgttg
    11101 atggatttag cggatgtcgt tattacccgt ggtggttcca atactctgtt tgagttgatt
    11161 gccatgcaac aactccattt gattgtgcct ctaggacgac aggccagtcg aggagatcag
    11221 attgaaaatg ctctctatgc agagaaaaag ggatattcta agcagattga tgaaagtcaa
    11281 ttgacatttg ccagcctgtt agtagaagtc gatgagctat tgaaacataa ggaattttat
    11341 gtccaaaaca tggcaaattc taatgaaatt cagtcagtag atagttttta caatttgctt
    11401 agagaagata tgggaaggta ggttctatga cggaaaaaga ttcaaatgta gaagaatcag
    11461 tgctagaagt ggaacaggca tcccaagtag aattggattc cgaacaaatt tcaccagcag
    11521 agaaagaatc tgtattagcc gaagagaaag aattttctac ggatgtagac atacctgaga
    11581 tgactgctcc ggatgatgaa aaaagtgcct ttttcgaaca atggaaagca cgccaccaag
    11641 cctaccttgc tcataaagat gaggcagata ttcaggcagt tgacgaggga cagacagaac
    11701 aggagatccc agaagctaaa aagagtaaaa gggtcctttt tcagggaata gaaagaaaaa
    11761 cagagagtct gaaatctaaa aaggaaacag gggaaaaggt tcaaacttta aaagttgata
    11821 ttccttccaa agtagtctgg aaagctattc cggttcttgt gacgagtttg ttactggcag
    11881 ctctagcctt gtattttatt tctccaacca gcaagaaaaa acaaatagag gttgtcggaa
    11941 atgagcgact aactgctgag caagtagaaa attatagcct tatctctccg gatgattaca
    12001 atgtgactat agctttgcat gcagatgctt acgcaaagaa tattaagaag aatagttcgt
    12061 ccgttgagac agcgacaatc aagttccagt ttccagctac ctttaccatt cacatcaaag
    12121 aatacgcaat aataggctac attcagcaac aaagtcagtg gtatccagta ttgtctagtg
    12181 gagagattgg cggtgaaccg atttcacagg attccttgcc agaagactat acaacaatca
    12241 atttatccga caaggaatta atcaaggaac tggccattga attgggtaaa atagatgcag
    12301 gaattcgttc tgctattcag acaataaatt taacgccaag taaagtgacc gcggatttgc
    12361 tgacattcaa tatgagagat ggaaatactg tattagtc
//
LOCUS       DBNFCK010000055        12391 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.055, whole genome shotgun sequence.
ACCESSION   DBNFCK010000055 DBNFCK010000000
VERSION     DBNFCK010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12391
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.055"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            44..1558
                     /gene="glpK"
                     /locus_tag="AC1PIW_001176"
     CDS             44..1558
                     /gene="glpK"
                     /locus_tag="AC1PIW_001176"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HIP6606116.1"
                     /translation="MSTEKYIMAIDQGTTSSRAIIFNKKGEKVGSYQKEFTQIFPKPG
                     WVEHNANEIWNSVQSVIAGSFIESGVRPDQIEGIGITNQRETTVVWDKETGLPIYNAV
                     VWQSRQTAHIADQLKADGHADMIHQKTGLVVDAYFSATKVRWILDQVPGAQERAEKGE
                     ILFGTIDTWLVWKLTDGKCHVTDYSNAARTMLYNIEELKWDDEILSLLNIPKAMLPEV
                     RSNSEVYGTTAPFHFYGAEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIVMNT
                     GEEMQLSQNNLLTTIGYGIGGKVYYALEGSIFIAGSAVQWLRDSMRMVTTSPESEELA
                     RKSTSNDEVYVVPAFTGLGAPYWNSDARGSVFGLTRGTTKEDFVKATLQSIAYQVRDV
                     IDTMQLDTGIDISVLKVDGGAAMNSLLMQFQADILGIEIARAQNLETTALGAAFLAGL
                     AVGFWKDLDELKELNAVGQSFQPTMNEARKEQLYKGWKKAVAATQLFAEVDEEQ"
     gene            1634..3463
                     /gene="glpO"
                     /locus_tag="AC1PIW_001177"
     CDS             1634..3463
                     /gene="glpO"
                     /locus_tag="AC1PIW_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HIP6606117.1"
                     /translation="MEFSKETRRLAIERMQDRQLDLLIIGGGITGAGVALQAAASGME
                     TALIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQNIAPHIPKPDP
                     MLLPVYDEPGSTFSLFRLKVAMDLYDLLAGVNNTDMANKVLTREEVLEREPNLKQEGL
                     IGGGVYLDFRNNDARLVIENIKRAAKDGALIASRAKAEKFIKDDAGKVVGIVARDLLT
                     DRTFEIRARLVINTTGPWSDEVRNLGGEGSGVLQMRPTKGVHLVVDSSRLSVPQPTYF
                     DTGHADGRMVFVLPRENKTYFGTTDTDYTGDLANPMVTQEDVDYLLDIVNNRFPEANL
                     TLDDIESGWAGLRPLLSGNGASDYNGGNNGKLSDDSFNSLIETVKGYMNNEKTRDDVE
                     HDLTHLEGSVSEKHLDPSAVSRGSALDRDDNGLLTLAGGKITDFRKIAEGAIEKVADI
                     LKEEHGRSFKLINSKTYPVSGGELNPANVAEEIEHLAQLGVKKGLLYDDALYLANLYG
                     SNAPKVFVLNHKVEAVSGLNKRDLLSLHYAMKEEMTLTAVDYLLRRTNYMLFMREQLD
                     AVALEIIKEMAAYYAWSEEEVAQQKQLLADTLVKNDLTYLKEK"
     gene            3477..4184
                     /locus_tag="AC1PIW_001178"
     CDS             3477..4184
                     /locus_tag="AC1PIW_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HIP6606118.1"
                     /translation="MTNELIGEVLGTTLLVLLGNGVVAGVVLDKSKAKDAGWIVITIG
                     WGLAVAMAAFVSGLLGTAHLNPAVSIAMAFAGNLPWASVVPYILAQFIGAFIGSILVY
                     LMYKDHYDATEDTGAVLATFSTGPAIRNLVSNTISEAIGTFVLVLGLLAFGQYDFPTG
                     IGTLIVGGLIVSLGVSLGGPTGYALNPARDLGPRIMHAILPLKHKGDSDWGYAWVPVV
                     GPIIGGLLAALLYGLIF"
     gene            4316..5266
                     /locus_tag="AC1PIW_001179"
     CDS             4316..5266
                     /locus_tag="AC1PIW_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,6-dichloro-p-hydroquinone 1,2-dioxygenase"
                     /protein_id="HIP6606119.1"
                     /translation="MNNTGIHHISSLVGNIHQAYHFYHHILGLKLTLKTVNQEDSSMY
                     HLFFGDEEGRYGTEFTIFDMPNHPNHRNGTNRLERTVFLVKNFEALEFWQKRLTEFEV
                     ENEGIQAFGNGHILNFQDEDGQLLGLTYHNAIGEMFPYKIDEIPSEYAILGIASLHMR
                     IREEKALLSLLTETFGFVEESRFVFEDKIVISLLFDNTFQHRLYLILDQESPISVMGV
                     GAIHHIAFGVLDESDLEDLISRLNLINRPHSGIINRDFIHSLYFRAPNYLMFEVATMT
                     GERESEMPAQNEELDQVDLFLPSFFEEDRQEIEKNLSQRY"
     gene            5489..5881
                     /locus_tag="AC1PIW_001180"
     CDS             5489..5881
                     /locus_tag="AC1PIW_001180"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIP6606120.1"
                     /translation="MRLSAFLDFWQDLDIRDYRQITFVGFSNGANFIMGLLAKQSNLA
                     DNYILLHPSALDYAFPMENSRAEILFTLGQNDQLVDQVALEHLVDDWQASAFPRANLV
                     RFDKGHFLSQDELTYVGNWYQERIDKKT"
     gene            complement(5906..7696)
                     /gene="pepF"
                     /locus_tag="AC1PIW_001181"
     CDS             complement(5906..7696)
                     /gene="pepF"
                     /locus_tag="AC1PIW_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HIP6606121.1"
                     /translation="MTNAMPKRQEIDVQLTWDTDILFPTPDNYKENLAAYAKQVTAFE
                     SNYKGKLTDKDTIVSALTEYEKIVILDSRLSHYAFLPLEVDKMNTELASLANEYDLVS
                     AKARPQRDFLYSELGQLDEAFLLELKEEQPQLAAFFDAILRDKPHQLHPLQEELLSNF
                     APTFGQPYNNYGVTKFEDMTFENFEANGETLGNSYVLFENDYELSHDTEIRRNSAAGF
                     YSTLKKYKNTTAATYLSHIKNEQIEARLRGFDNTIDFLLHSQNVSRDLFDRQIDVIMK
                     ELAPHMRRYVKLVAKAHGLDKITHADLKISLPSEYNQRITPEESKQFLIDCLGILGED
                     YVKMIEQSFDERWIDFAQNEGKATGGFCATLYDGPSYILLSWTGLMNEVLVLAHELGH
                     AGHFQLAKKQSVLSYDPSLYFIEAPSTANEVLTCNTLLKNNQDPGFQAYLISELISRT
                     YFHNMVTHLLEAAFQREVYTRLDNEEYLNGDILCQIKLDVIKEFWGEDFEIGDDAGLI
                     WMRQPHYYIGLYPYTYSAGLTIGTAMAKQLEEHPEEVVEKWLETLSLGASLSAQDLAK
                     HAGVDVSTDQPLKETIAYVGSLVDKLETLI"
     gene            7863..8201
                     /locus_tag="AC1PIW_001182"
     CDS             7863..8201
                     /locus_tag="AC1PIW_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002264292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HIP6606122.1"
                     /translation="MQVIKRSGEIVEFDPDKIYQAIIKAAQTVYVIDETWRKNLAQVT
                     KKVVLDLEEAHTERPTISMVQSQVEHRLLDAGYISIAEHYISYRLQRDLERNGYGDKI
                     IVHLRFEQVR"
     gene            8386..9753
                     /gene="nox"
                     /locus_tag="AC1PIW_001183"
     CDS             8386..9753
                     /gene="nox"
                     /locus_tag="AC1PIW_001183"
                     /EC_number="1.6.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H2O-forming NADH oxidase"
                     /protein_id="HIP6606123.1"
                     /translation="MAKIVVVGANHAGTAAIKTMLTNYGQENEIVVFDQNSNISFLGC
                     GMALWIGEQIGGPEGLFYSNKEELESLGATVYMESPVTNIDYDAKTVTALVNGQEHVE
                     SFEKLLFATGSQPILPPIKGAEIKEGSLEFEATLENLQFVKLYQNSADVIEKLKNKDI
                     NRVAVVGAGYIGVELAEAFQRKGKEVVLIDVVDTCLAGYYDRDLSDLMAKNLEEHGIK
                     LAFGETVKEVAGNGKVEKIITDKNEYDVDMVILAVGFRPNTALAGEGIERFRNGAFLV
                     NKRQETSIPGVYAIGDCATIYDNATGDTSYIALASNAVRTGIVAAHNICGTDLEGIGV
                     QGSNGISIYGLNLVSTGLTLEKATRLGLNAAVTEVTDNQKPEFMEHGNFPVTIKIVYD
                     KDSRRILGAQMAAREDISLGIHLFSLAIQEGVTIEKLALTDLFFLPHFNKPYNYITVA
                     ALGAE"
     gene            10042..12321
                     /gene="pcrA"
                     /locus_tag="AC1PIW_001184"
     CDS             10042..12321
                     /gene="pcrA"
                     /locus_tag="AC1PIW_001184"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HIP6606124.1"
                     /translation="MNPLLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKMVNPWNILAITFTNKAAREMKERAYALNPATQACLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRSLMKRILKNLNLDPKKWSERSILGTISNAKNDLIDDKAFEAQA
                     GDLYTQIVAKCYTAYQKELRQSEAVDFDDLIMLTLRLFDQNPEVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPESKVVL
                     LEENYRSTKNVLQAANEVIENNRNRRPKKLWTQNAQGDLITYYRARDENDEAIFVAGQ
                     IDQLHREGKAYKDFTVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDVISY
                     LNIIANPSDNISYERIVNEPKRGVGPGTVDKLRDFATSHSMSLLEASQDIMLSPVKGK
                     AAQAVFDLANLLYNLRNQLDNLTVTQVVEAVLNQTGYIDALAAQNTLEANARIENIQE
                     FLSVTKNFDEKDVEEEETGLDKLTRFLLDLALIADTDDGDTESSEVTLMTLHAAKGLE
                     FPVVFLIGMEENVFPLSRAAEEEDELEEERRLAYVGITRAEQTLYLTNANSRLLFGRT
                     NYNQPSRFIREISSDLLDYQGLARPANTSFKASYVNGRATQFGQGMSLQQALQSRKAS
                     VQPSAKLGGSMPFASSNQSSSSGTNWSVGDIAVHKKWGRGTVLEVSGSGSNQELKINF
                     PDMGLKKVLASLAPISKED"
BASE COUNT         3592 a         2517 c         2783 g         3499 t
ORIGIN      
        1 ggaagcgttt tcaaaaaaag ataaacaaac aggagggaga cctatgtcta ctgaaaaata
       61 tatcatggcc attgaccaag gaactacaag ttcacgtgcc attatcttca ataaaaaagg
      121 tgaaaaggtt ggcagttacc aaaaagagtt tacgcaaatt ttccctaaac caggttgggt
      181 tgaacacaac gccaatgaaa tttggaattc tgttcagtct gtgattgcag ggtcctttat
      241 cgaaagcggt gttcgtcctg atcagattga aggaattggt attaccaacc agcgtgaaac
      301 aaccgttgtc tgggataagg aaacaggtct tccaatctac aatgccgttg tctggcaatc
      361 tcgtcaaaca gctcatattg ctgaccagct gaaggcggat ggtcatgcag atatgattca
      421 tcaaaagaca ggtctagtag tcgacgccta cttctcagca accaaagttc gttggatttt
      481 agatcaagtc cctggagcgc aggaacgtgc tgaaaaaggt gagattctct ttggaaccat
      541 tgatacttgg ttggtttgga aattgactga tgggaaatgc catgtgacgg attattcaaa
      601 cgcagctcgt accatgctct acaacattga agagttgaaa tgggatgatg aaattttatc
      661 tcttctcaat attccaaaag ccatgctgcc agaagttcgt tcaaactctg aagtttacgg
      721 cacaacagct ccattccatt tctatggcgc agaagtgcca atctctggca tggcaggtga
      781 ccaacaggct gccttatttg gacaattagc atttgagcca ggtatggtga aaaatactta
      841 cggaaccggt tccttcatcg tgatgaatac aggtgaagag atgcagttgt cacagaacaa
      901 ccttctcaca acaattggct atggtatcgg tggcaaagtc tattatgcgc ttgaaggatc
      961 tatctttatc gctggttcgg cggttcaatg gctccgtgac agcatgcgaa tggtgactac
     1021 atcacctgag tcagaagagc ttgctcgtaa gtctaccagc aatgatgaag tctatgttgt
     1081 tccagccttt acaggacttg gtgctccgta ttggaactca gatgcgcgtg gctcagtctt
     1141 tggtctcact cgtggtacga ctaaagaaga cttcgtcaaa gcaactcttc aatccattgc
     1201 ctaccaagtt cgagatgtta ttgacaccat gcaattggat acaggtattg atatttctgt
     1261 cttgaaggtt gacggtggtg cagcaatgaa cagcctcctc atgcaattcc aagcagatat
     1321 tttgggtatt gaaatcgcac gcgctcaaaa ccttgaaact acagctcttg gagcagcatt
     1381 cttagcaggt ttagcagttg gtttctggaa agatttggat gaattgaaag aactcaacgc
     1441 tgtcggccaa tccttccaac caaccatgaa cgaagctcgt aaagaacaac tttacaaggg
     1501 atggaaaaaa gctgtcgcag caactcaact atttgcagaa gtggatgaag agcaataaag
     1561 gacttgttag ctaggggaaa gaagaatttc tgcttgctag acagtcctat tcgaaagtta
     1621 ggaagtgaat cgtatggaat tttcaaaaga aacaagacgc ttagctattg agcgaatgca
     1681 ggatcgtcaa cttgacctcc tcattatcgg gggtggtatt acaggtgcag gtgtggcttt
     1741 gcaggcagca gcaagcggta tggaaacagc cttgattgaa atgcaggact ttgcagaagg
     1801 aacttccagt cgctcaacca aattggtcca cggtggtctt cgctacctca aacaatttga
     1861 cgtggaagtg gtatccgata ccgtatctga acgtgctgta gtacaaaata tcgccccaca
     1921 tattccaaag ccagacccaa tgcttttacc agtctatgat gagccaggtt ctaccttcag
     1981 cctcttccgt ttgaaagtgg ccatggactt gtatgatctc ttggctggcg tcaataatac
     2041 cgatatggcc aataaggtct tgacaagaga agaagtactt gaacgcgaac caaacttgaa
     2101 gcaagaagga ttgattggtg gcggtgtcta tcttgatttc cgcaacaatg atgctcgttt
     2161 ggttattgaa aatatcaaac gtgcagccaa ggatggtgcc ttgattgcca gccgtgccaa
     2221 agctgagaaa ttcatcaaag atgatgctgg aaaagttgtc ggtatcgtcg ctcgtgattt
     2281 gctgacagac cgcacctttg aaatccgtgc gcgtttggtc atcaatacaa caggaccatg
     2341 gagtgatgaa gtgcgtaatc tgggtggaga aggttcaggc gttctccaga tgcgtccgac
     2401 caagggtgtg cacctagttg tcgatagctc tcgcttgtct gtaccacagc cgacttactt
     2461 tgatactggc catgcggatg gtcgaatggt ctttgtcctt ccgcgtgaaa ataaaaccta
     2521 ttttggtacg actgatacag actatactgg tgatttggca aatccaatgg taacccaaga
     2581 agatgttgac tacctgcttg atattgtcaa caatcgcttc ccagaagcaa acttgaccct
     2641 ggatgacatt gagagtggtt gggcaggtct tcgtcccctc ttgtctggaa atggtgcttc
     2701 tgactacaat ggtggaaaca acggtaagct cagcgatgac agctttaata gcttgattga
     2761 aacggtcaaa ggttatatga acaatgaaaa gacacgtgac gatgtggaac atgatttgac
     2821 ccatctggaa ggcagcgttt ctgaaaaaca cttggatcct tcagctgtat cacgtggctc
     2881 tgcccttgac cgtgatgaca atggtttgct gactcttgct ggtggtaaga ttactgactt
     2941 ccgtaagata gcggaaggtg caattgaaaa agtggcagac attctcaaag aagaacatgg
     3001 tcgcagcttc aaactcatca attctaagac ctatccagtc tctggtggtg aattgaaccc
     3061 tgcaaacgta gcagaagaaa ttgaacatct tgctcaacta ggtgtgaaga aaggtctcct
     3121 ttatgatgac gccctttact tggcaaactt gtacggttca aatgctccaa aagtctttgt
     3181 attaaaccat aaagtagaag ccgtttctgg cttgaacaag cgtgatttgc tttcccttca
     3241 ctatgctatg aaggaagaga tgaccttgac tgcagttgac tatcttcttc gtcgtaccaa
     3301 ctacatgctc tttatgcgtg agcagttgga tgcagttgcg cttgaaatca ttaaggaaat
     3361 ggcagcttat tatgcttggt cagaggaaga agttgctcaa cagaaacaat tgttggcaga
     3421 tacacttgtt aagaatgatt tgacctattt gaaagaaaaa tagaaagagg tgcatgatga
     3481 caaatgaatt aatcggtgaa gtgttgggaa ccaccttact tgtcttacta ggtaatggtg
     3541 tggtagcagg agtagtacta gataagagta aggccaagga tgctggttgg attgtaatca
     3601 caattggttg gggtctagca gtagccatgg cagcttttgt gagtggttta ttgggaactg
     3661 cccacctcaa cccagctgta tcaattgcaa tggcctttgc aggaaatctc ccatgggctt
     3721 ctgtagtccc ttacatcctt gctcaattta tcggagcctt tatcggaagt atcttggtct
     3781 acctcatgta caaagatcac tacgatgcaa cagaagacac tggtgctgtg ttggcaactt
     3841 tctctactgg acctgccatc cgcaaccttg tttctaatac aattagcgaa gcaatcggaa
     3901 cctttgtttt ggtcttggga ttactggctt tcggtcagta tgattttcca acagggattg
     3961 gcactttaat cgtcggtggt ttgattgtct cactcggagt ttctcttggt ggtccaacag
     4021 gatatgccct aaaccctgcg cgtgatcttg gtcctcgtat catgcatgcc atccttccat
     4081 tgaagcacaa gggagattcg gattggggct atgcatgggt accagtagtt ggtccaatca
     4141 tcggaggctt gcttgcggct ctcctttatg gtctcatttt ttaaaaaata tccttctcta
     4201 atcagcaatg gttagggaag ttttttatta cttggctgtc aatcgtgagc aaaagatttt
     4261 tatttatcaa taattagtga taaaatatgt ctatcaacat cagtgaggtc atcttatgaa
     4321 caatacaggt attcatcaca tttcaagctt ggtaggaaac atacaccaag cctatcattt
     4381 ttatcatcat attctcggct taaaattgac cttgaaaact gtcaatcaag aagattcaag
     4441 catgtatcat ctctttttcg gtgatgaaga gggccgctat ggaacggaat ttaccatttt
     4501 tgatatgcct aaccatccca atcatcgaaa cggaaccaat cgtttggaaa gaactgtttt
     4561 tctggtaaaa aattttgagg cattagaatt ctggcagaaa agactgacag agtttgaagt
     4621 ggagaatgaa ggaattcagg cttttggaaa tgggcatatt ctgaatttcc aagatgaaga
     4681 tggccagttg cttggcttga cctatcataa cgctatcgga gaaatgtttc cgtataaaat
     4741 agatgaaatt ccgtcggaat acgctattct tggcattgcc agtcttcaca tgcgaatacg
     4801 tgaagaaaag gcattactaa gtttgctgac agaaaccttc ggatttgttg aggaaagtcg
     4861 atttgtcttt gaggataaga tagtgatttc tcttctcttt gataacacct tccaacatcg
     4921 actttatttg atacttgacc aagagtcgcc aatcagcgtt atgggagtcg gggccattca
     4981 tcatatcgca tttggtgtct tagatgagtc cgacttggaa gacctgatta gtcgcttaaa
     5041 tctcatcaat cgtccacatt ctggtatcat taatcgagat ttcattcatt ccttgtactt
     5101 ccgagcgcca aattacctta tgtttgaggt ggcaaccatg actggtgagc gcgaatcgga
     5161 aatgccagct cagaatgaag agttggatca ggtagaccta tttttgccaa gtttctttga
     5221 agaggataga caggaaatcg aaaaaaatct ctcccaacgt tattaggaga aatgaagtat
     5281 ggattatcgc tttatcaaag ggaccagtcc cagtcttttg gtctttttcc acggcacagg
     5341 tggcaacaag gaatccatgc tctttcttcg ctaacagctg gatccagaag cttttgtcct
     5401 ttccttagat ggaagttggg gtcaaggcag ggaaaggcgt ttctttgcac ctctcattga
     5461 cggtcagctg gatcttgtcg atgttgaaat gcgcttgtcg gcttttctgg atttttggca
     5521 ggacttggac atccgagatt atcgtcagat tactttcgta ggtttttcaa acggtgctaa
     5581 ctttatcatg ggcttgttgg ccaagcagtc aaacctggcg gacaattata tcttgctcca
     5641 cccttcggcc ttagattacg cttttcctat ggaaaatagc agagcagaga ttttatttac
     5701 actggggcag aatgaccaac tcgtcgacca agtcgctctt gaacatcttg tagatgactg
     5761 gcaagcgtca gcttttccca gagccaacct agttcgtttt gacaagggac attttttaag
     5821 tcaggatgaa ttgacctacg tgggaaattg gtatcaagaa agaatagaca aaaagacctg
     5881 aacaagttca agtcttttat gtgtcttaga ttagtgtttc caatttatcc accaatgaac
     5941 caacataggc aatggtttct ttgagtggtt ggtcagttga aacgtcaaca cctgcgtgct
     6001 tagcaaggtc ttgggcagaa agacttgcac cgagtgacaa ggtttccaac catttttcaa
     6061 caacttcttc aggatgttct tccaattgtt tcgccatagc cgtaccgatg gtcaaaccag
     6121 ctgagtaggt atatggatac aaaccaatgt agtagtgtgg ttgacgcatc cagataagac
     6181 ctgcatcgtc accgatttca aagtcttcac cccagaattc cttgatgaca tccaacttga
     6241 tttggcagag aatatcaccg ttgaggtatt cttcattgtc caaacgagtg tagacttcac
     6301 gttggaaggc tgcttcaagc aagtgagtga ccatgttgtg gaagtaagta cggctgatca
     6361 actcgctaat caagtaagcc tggaagccag ggtcttggtt gtttttcaaa agggtgttac
     6421 atgtcaagac ttcatttgca gtagaaggcg cctcgatgaa gtagagggat ggatcgtagc
     6481 taaggacact ttgtttctta gccaattgga aatgaccagc atgtcccaat tcgtgagcca
     6541 ataccaagac ctcgttcatc aagcctgtcc atgaaagtag gatgtaagat ggtccatcgt
     6601 aaagagtagc acagaagcca ccagtagcct taccttcgtt ttgagcaaag tcaatccaac
     6661 gctcatcaaa tgattgttca atcattttca cataatcttc accaagaatg cccaggcagt
     6721 caatcaagaa ttgtttggat tcttcaggtg tgatgcgttg gttgtattca gatggtagac
     6781 tgattttcaa gtcagcatgt gtgatcttgt ccaatccatg agccttagca accaatttta
     6841 catagcgacg catgtgagga gccaattctt tcatgatgac atcaatctga cggtcgaaga
     6901 gatcacgaga gacattttga ctatgcaaga ggaagtcaat cgtattgtca aatccacgca
     6961 agcgagcttc gatttgctca ttcttgatat gagacaaata agttgctgca gttgtattct
     7021 tgtatttttt caaggttgaa tagaaaccag cagctgaatt gcgacggatt tcggtatcat
     7081 ggctgagctc ataatcattt tcaaagagca catagctatt gccgagcgtt tcgccatttg
     7141 cctcgaagtt ttcaaatgtc atatcttcaa acttggtcac accgtagttg ttatagggct
     7201 gaccaaatgt tggtgcaaaa tttgataaaa gttcttcttg aagtgggtgc agttgatgcg
     7261 gcttgtcacg gagaatagca tcaaagaatg cagccaattg tggttgttct tcttttaatt
     7321 caagtagaaa ggcttcatct aattgaccaa gttctgaata gaggaaatct cgttgaggac
     7381 gtgccttggc agaaacgagg tcgtattcgt tggccaggct cgccaattca gtattcatct
     7441 tatcgacttc caaaggaagg aaggcatagt gggaaagtct gctatcaaga ataacgattt
     7501 tttcgtattc tgttaaggcg gaaacgatgg tatccttatc agttagcttt cctttgtaat
     7561 tcgattcgaa agctgtcact tgcttagcgt aggctgccaa attttctttg tagttatcag
     7621 gggtcggaaa tagaatgtcc gtatcccaag ttaattgcac atcaatttct tgacgtttag
     7681 gcattgcatt tgtcatgatt ttctccttat ttcattttaa taggtccatt atatcatact
     7741 aaagggcttt cagcgaaaat aagccaaggc aaaatttatg aaaaagaata cttgacaaaa
     7801 tacacaaaaa cctagataat gtaagtatga aagggtttac tgaaaaccag aaaggagctt
     7861 ttatgcaagt cattaaacgt agtggagaga ttgttgaatt tgatccagat aaaatctacc
     7921 aagccatcat taaagctgca cagacagtct atgtcattga tgaaacttgg cggaaaaatc
     7981 ttgctcaggt cacaaagaaa gtagtgcttg acttggaaga agctcataca gagcgaccaa
     8041 cgattagcat ggttcagtcg caagttgaac atcgtctttt agatgcaggg tatatttcta
     8101 ttgctgagca ttacatttcc tatcgcttgc aacgtgattt agaacgtaac ggctatggcg
     8161 ataagattat tgtacatctg cgttttgaac aagtacgtta agagaaacct agagaaatct
     8221 aggtttttgc ttttatatat ctttacattg tttaaagaaa atagcatttc aaaaactttt
     8281 tgaaaaaaat gtgatattct gagcatattt tttgaaatcg gtaacattta tattgtataa
     8341 tatagttcgt aaaaaaatat attttcgaaa gtgagatttt acattatggc taaaatcgtt
     8401 gttgtcggtg ctaaccatgc tggtactgcc gcaatcaaaa ctatgttgac aaattatggt
     8461 caagaaaatg aaatcgttgt atttgaccaa aactcaaata tttcattctt gggttgtggt
     8521 atggctttgt ggatcggtga gcaaattggc ggtcctgaag gacttttcta ctcaaacaaa
     8581 gaagagttgg agagcttggg tgcgacagta tacatggagt cacctgttac aaacattgac
     8641 tatgatgcca aaacagttac agcgcttgta aatggtcaag aacatgttga atcatttgaa
     8701 aaacttcttt tcgcaactgg ttcacaacct atcttaccac caatcaaagg agctgaaatc
     8761 aaagaaggtt cgcttgaatt tgaagcaact cttgaaaact tgcaatttgt taaattgtac
     8821 caaaactcag cagatgttat tgaaaaattg aaaaacaaag acatcaaccg cgtcgcagtt
     8881 gttggtgctg gttacatcgg tgttgagctt gcggaagcat tccaacgtaa aggtaaagaa
     8941 gtagtactta ttgatgttgt ggatacatgt ttggctgggt actatgaccg tgacttgtca
     9001 gaccttatgg ctaaaaactt ggaagagcat ggtatcaaac ttgccttcgg cgaaactgtt
     9061 aaagaagttg ctggtaatgg taaagttgag aaaatcatca ctgacaagaa cgaatacgat
     9121 gttgacatgg ttatcttggc tgttggtttc cgtccaaaca ctgcgcttgc aggtgaagga
     9181 atcgagcgct tccgtaatgg tgccttcctt gtaaacaaac gtcaagaaac ttctatccca
     9241 ggcgtttacg ctatcggtga ctgtgcaaca atctacgaca acgctactgg cgatacaagc
     9301 tacatcgctt tggcttcaaa tgcagtacgt actggtatcg tagcagctca caacatctgt
     9361 ggcactgacc ttgaaggtat cggtgtacaa ggttctaacg gtatctctat ctacggtctt
     9421 aaccttgtat caacaggttt gacacttgaa aaagctactc gtcttggttt gaacgctgct
     9481 gttactgaag taacagacaa ccaaaaacca gaattcatgg agcacggcaa cttccctgtc
     9541 actatcaaga ttgtttacga taaagattca cgtcgtatcc ttggtgcgca aatggctgct
     9601 cgtgaagaca tctcattggg tatccacctc ttctcattgg caatccaaga aggtgtaaca
     9661 atcgaaaaat tggccttgac agacttgttc ttcttgccac acttcaacaa accatacaac
     9721 tacatcacag tagctgcctt gggtgctgaa taattaattg aatacaaaag ctcagagatg
     9781 actctgggct ttttttgctt gccatctatc acattgaact gtctatcgag agattttttg
     9841 gtataatgga gagaatgatt ataacgaacc tgtattcaca gtgtagtact tccatctaag
     9901 cctagttttt cagctattca tcgtttgttt ttcaaaaaat acagtcggta ttccttcaaa
     9961 aaactgcctt gattagcgaa aaactatctc ttatttaaaa gcactccact tgtaatacat
    10021 gttctaagaa agtatgagaa aatgaaccca ttattaaacg gaatgaatga cagacaggca
    10081 gaagcggtac agacgacgga aggtccgctt ttgattatgg caggggctgg ttcgggtaag
    10141 acgcgagtcc tgacccaccg tatcgcttat ttgattgatg aaaaaatggt caatccttgg
    10201 aatatcctag ccattacctt taccaacaag gcggcacggg agatgaagga gcgggcctat
    10261 gccctcaatc ctgccacgca agcctgtttg atagctacct tccactccat gtgtgtgcgg
    10321 attttgcgtc gggatgctga ccatatcggt tacaatcgca attttacgat tgtcgatcca
    10381 ggtgagcaac gcagtctcat gaaacggatt ttaaaaaatc tcaatctaga tccgaaaaag
    10441 tggagtgaac gttctatcct gggcaccatt tccaatgcca aaaacgactt gattgatgac
    10501 aaggcatttg aagcgcaggc aggcgaccta tacacgcaga ttgttgctaa atgctacacg
    10561 gcttatcaaa aggaactaag acagtcagag gccgttgact ttgatgattt gattatgttg
    10621 acgctccgcc tttttgacca aaatccagag gtcctgacct attaccagca gaaattccag
    10681 tatatccatg tcgatgagta tcaggatacc aaccatgccc agtaccaatt agtcaagctc
    10741 ctggcatcac gtttcaagaa tatctgcgtg gtcggagatg cggaccagtc tatctatggt
    10801 tggcgtggtg cggatatgca aaatatcctg gactttgaaa aggactatcc agagagcaag
    10861 gttgtgcttt tggaggaaaa ctatcgttcg accaagaacg tcttgcaggc tgccaatgag
    10921 gtcatcgaaa acaaccgcaa tcgacgtccc aaaaaactct ggacacagaa tgcccaaggg
    10981 gatttgatta cttactatcg tgccagagat gagaatgacg aggcgatttt tgtcgcaggg
    11041 cagattgacc agttgcaccg agaaggcaag gcttataagg attttacagt tctctaccgt
    11101 accaatgccc aatcacgtac cattgaagaa gccctgctca aatctaatat cccttatacc
    11161 atggttggag gaaccaagtt ctacagccgt aaggaaatcc gggatgtgat ttcttatttg
    11221 aatatcattg caaatccatc ggataatatc tcctatgaac gcattgtcaa tgagcccaaa
    11281 cgtggcgttg gacctggtac ggttgacaaa ctccgtgatt ttgcgaccag ccacagtatg
    11341 tccttgctag aagcttctca ggatattatg ttgtcaccag tcaagggcaa ggcagcgcag
    11401 gcagtctttg acctagccaa tctcctctat aatcttcgca atcaattaga caacttgacg
    11461 gttacccaag tagtcgaggc agtattgaac caaacaggct acatcgatgc ccttgcagca
    11521 caaaataccc tggaagcaaa tgcccgcatt gaaaacatcc aggaattcct ttctgttacc
    11581 aaaaactttg atgaaaagga tgttgaggaa gaagagacag gtctagataa attaacacgc
    11641 ttcctgcttg atttggccct gattgctgac acagatgatg gcgatacaga atcttcagaa
    11701 gtgactctta tgaccctcca cgcagccaaa ggtctggaat ttccagttgt cttcttgatt
    11761 ggtatggagg aaaatgtctt cccacttagt cgtgcagcag aagaagagga cgaattggag
    11821 gaagaacgtc gtttggcata cgtaggcatc acccgtgctg agcagactct ctacctgacc
    11881 aatgccaatt ctcgactcct tttcggtcgc accaactaca accaaccaag ccgctttatt
    11941 cgagaaattt ccagtgactt gcttgattat caaggcctcg ctcgtccagc taatactagc
    12001 tttaaggctt cttatgtcaa tggcagagcg acacagtttg gacaaggcat gagcttgcaa
    12061 caagccctac aaagtcgaaa agcatccgta caaccatctg ccaagctcgg aggtagtatg
    12121 cccttcgcat caagcaacca atcgtcaagc tcaggcacca actggtcagt tggtgacatc
    12181 gcagtccata agaaatgggg ccgcggaaca gtccttgaag tatcaggttc aggcagcaat
    12241 caagaactca aaatcaattt ccctgatatg ggactcaaaa aagtcttggc aagcttagcg
    12301 ccgataagta aagaagatta aatttagtta aaaaataatt gcagtaagct cgaaaatatg
    12361 ataaagtatt tttgtaaaac tgaatagaac a
//
LOCUS       DBNFCK010000056        12207 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.056, whole genome shotgun sequence.
ACCESSION   DBNFCK010000056 DBNFCK010000000
VERSION     DBNFCK010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12207
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.056"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..508)
                     /locus_tag="AC1PIW_001185"
     CDS             complement(<1..508)
                     /locus_tag="AC1PIW_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606125.1"
                     /translation="MFLFAFPGMGKTTLAKKYTDVVDLEMSDIKYDNSSVRHLTKEER
                     KSTKRPLKDKRYKTIYVNKGYTLHRQGKTVLVALNFLMRMLLAMSIRGGVPFHIYIPH
                     PSLKEEYRQRYISRGNNDRFIFEVMFIWYPALIPLYLLSKILPKWITVTQSGETLEDY
                     YKRENFVRF"
     gene            complement(646..737)
                     /gene="ffs"
                     /locus_tag="AC1PIW_001186"
     ncRNA           complement(646..737)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="AC1PIW_001186"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(779..1300)
                     /gene="tadA"
                     /locus_tag="AC1PIW_001187"
     CDS             complement(779..1300)
                     /gene="tadA"
                     /locus_tag="AC1PIW_001187"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HIP6606126.1"
                     /translation="MNYTHEEKEYFMTQALQEARKSLEKDEIPIGCVIVKDGQIIGRG
                     HNAREELNQAIMHAEVMAIQEANNVEGNWRLLDSTLFVTIEPCVMCSGAIGLARIPQV
                     IYGATNKKFGAAGSLYDILTDERLNHRVKVETGILEAECANIMQDFFRQRRESQKAAK
                     LIMKTQEEATSDS"
     gene            complement(1580..3550)
                     /gene="tkt"
                     /locus_tag="AC1PIW_001188"
     CDS             complement(1580..3550)
                     /gene="tkt"
                     /locus_tag="AC1PIW_001188"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIP6606127.1"
                     /translation="MSNLSVNAIRFLGIDAIEKSKSGHPGVVMGAAPMAYSLFTKELR
                     INPAQPNWVNRDRFILSAGHGSMLLYGLLHLSGFEEVTMDEIKNFRQWGSKTPGHPEF
                     GHTAGVDATTGPLGQGISTATGFAQAERFLAAKYNRDGFPIFDHYTYVICGDGDLMEG
                     VSAEAASYAGLQKLEKLIVLYDSNDINLDGETKDSFTEDVRARYNAYGWHTDLVTDGT
                     DVDAIFAAIEKAKVAGKPSLIEIKTVIGHGSPNKQGTNAVHGAPLGAEETEATRKALD
                     WNYGPFEIPAEVYADFKANVADRGAAAYDAWVKLVEDYKVAHPELAAEVTAILEGRDV
                     VEIKPEDFPVYENGFSQATRNSSQDALNAAAKVLPTFLGGSADLAHSNMTYIKEDGLQ
                     DSVNPLNRNIQFGVREFAMGTILNGMAAHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPIEHLAGLRAMPNLNVFRPADARETQAAWYLSLTSKS
                     TPSALVLTRQNLTVEEGTDFDKVAKGAYVVYEAAGFDTILLASGSEVNLAVKAAKELE
                     AAGTKVRVVSVPSTELFDAQDAAYKEEILPNAIRRRLAIEMGATQSWYKYVGLDGKVL
                     GIDTFGASAPAQTVIDNYGFTVENVVKLVGEL"
     gene            complement(3761..4852)
                     /gene="ulaG"
                     /locus_tag="AC1PIW_001189"
     CDS             complement(3761..4852)
                     /gene="ulaG"
                     /locus_tag="AC1PIW_001189"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HIP6606128.1"
                     /translation="MAKVQDITRESWILSTFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGVWIKTPGGANVVMDLWSSRGKSTKKVKDMVWGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHVHSDHMDISTAAAIINNPKLDHVKFVGPVESGDIWEKWGVPAD
                     RIIRIAPGDSFEFKDIKVHAVESFDRTCLVTLPIEGYDENGGKLAGLPVTDELMARKA
                     VNYVFETPGGTIYHGADSHFSNYFAKHGRDFNIDVAINNYGDNPIGIQDKMTSIDLLR
                     MAENLRAKVIIPVHYDIWSNFMASTDEILQLWKMRKERLQYDFHPFIWEVGGKYTYPM
                     DKDRIEYHHPRGFDDCFLEDSNIQFKALL"
     gene            complement(4962..6650)
                     /locus_tag="AC1PIW_001190"
     CDS             complement(4962..6650)
                     /locus_tag="AC1PIW_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HIP6606129.1"
                     /translation="MLLDKSSCALLRYLIGLQEPETIMAISKGLQQSRRKIYYHLEKI
                     NDALPTTVESIESLPRLGIRLTEAQKLACRDLLDSIDSYTYVMSMPERIQLMLVYICI
                     TPERVTIERLMELTEVSRNTVLNDLNEIRTQLAQEQYKMTLYVSKSHGYSLVCHPLNK
                     IQYVHSLLYTIFLDANKGFLQVLEAKLFELVGCQLLFSEEMAQFLQGQVSQLEKKLGK
                     KINRYEIEFMLKVLPFLLLSYRNMELDDLEERAILKEFEQVRERIEYRVAQELKDALL
                     QTFDLKLDEIEISLIAILLLSYRKDSDVHVFSQDFIDLKRVVERFIHHFEIHSPFELE
                     NPDELAKHLLAHCKALLFRKTYGILSKNPMVQQIQDKYSTLFAMTRVSGKILEEEWQI
                     ELSDGDIAYLTIHLGGAIRRSGTRKAQTQIVYLICDEGVSVQRLLYKQCQHHLPDKKI
                     GAVFTTEQFKSVEDLLEVDFLITTSDGLETDFPLVQVHPILDFDDVLNMTHFAKYRSL
                     ADEKQVFAVELDKLLSGYIADGRTARELKQRLQKLITNELLTNLSSQDIETDLY"
     gene            complement(6775..7392)
                     /locus_tag="AC1PIW_001191"
     CDS             complement(6775..7392)
                     /locus_tag="AC1PIW_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606130.1"
                     /translation="MRKFLQDSLTETAYYLELILSAILGLALLALSTLLFADLLSSLA
                     GDIQADTFIQNFLSRAMTLAVGVEFIKMLCKQSPSTVIEVLLVAIARQLIVEHGSSSD
                     YLIGIISVAILFAVRKYLFTQFDDSNNIIMRASQKVKVANIIARVKIPSEGSETLRDF
                     VTRKLNEEEKTIAIGACVYLKNVALRIDNMHGELITRVEIIKSIY"
     gene            complement(7477..8193)
                     /locus_tag="AC1PIW_001192"
     CDS             complement(7477..8193)
                     /locus_tag="AC1PIW_001192"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HIP6606131.1"
                     /translation="MPKDLQEMRQRVYEANIALPAHGLVKFTWGNVSEVCRELGRIVI
                     KPSGVDYEKLSPENMVVTDLDGNVVEGDLNPSSDLATHVALYKAWPNVHAVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGPVPCARSLTAEEVNSAYEKETGSVIIEEFERRGI
                     DPVAVPGIVVRNHGPFTWGKNPAQAVYHSVVLEEVARMNRYTEQINPRVEPAPSYIMD
                     KHYLRKHGPNAYYGQKGDEH"
     gene            complement(8193..9056)
                     /locus_tag="AC1PIW_001193"
     CDS             complement(8193..9056)
                     /locus_tag="AC1PIW_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HIP6606132.1"
                     /translation="MARPIGIYEKATPKHFTWKERLEFAKELGFDFVEMSVDESDARL
                     ARLEWTKEERLDLVKAIYETGVRIPTICFSGHRRYPLGSNDPALEAKSLETMKQCIEL
                     AQDLGVRVIQLAGYDVYYEEKSPETRERFIKNLRQACDWAEQAQVMLGIEIMDDPFIN
                     SIEKYLAVEKEIDSPYLFVYPDTGNVSAWHNDIYSEFYLGHRSIAALHLKDTYAVTEN
                     SKGQFRDVPFGQGCVDWDNMFAVLKKTNYQGPFLIEMWSENCETVEETRAAIKEAQDF
                     LYPLIEKAGLN"
     gene            complement(9062..9724)
                     /locus_tag="AC1PIW_001194"
     CDS             complement(9062..9724)
                     /locus_tag="AC1PIW_001194"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014622185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HIP6606133.1"
                     /translation="MKHIPNLQVALDHSDLQGAIKAAVSVGHEVDVIEAGTVCLLQVG
                     SELVEVLRSLFQDKIIVADTKCADAGGTVAKNNAVRGADWMTCICSATIPTMAAALKA
                     IKEVRGDKGEIQIELYGDWTYEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNKV
                     KKLVDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAAAAREFKDEIRRI
                     WG"
     gene            complement(9735..10220)
                     /locus_tag="AC1PIW_001195"
     CDS             complement(9735..10220)
                     /locus_tag="AC1PIW_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIP6606134.1"
                     /translation="MNLQKSFTENNSIRLGLTAETWQEAVHKAVEPLIESGAATEEYY
                     DAIIASTEEYGPYYVLMPGMAMPHAQAGVGVLKDSFALITLTKPVVFSDGKEVSVLLT
                     LAATDPKIHTSVAIPQIIALFELENSIERLIACQTPEEVLAMVEESKNSPYLEGLDLE
                     S"
     gene            complement(10283..10561)
                     /locus_tag="AC1PIW_001196"
     CDS             complement(10283..10561)
                     /locus_tag="AC1PIW_001196"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIP6606135.1"
                     /translation="MVKVLTACGNGMGSSMVIKMKVENALRQLGVTDFQSASCSVGEA
                     KGLAAGYDIVVASNHLISELEGRTKGHLVGLDNLMDDNEIKTKLQAVL"
     gene            complement(10620..12068)
                     /locus_tag="AC1PIW_001197"
     CDS             complement(10620..12068)
                     /locus_tag="AC1PIW_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HIP6606136.1"
                     /translation="MDFLQTPLNWFSQNILQNPAFFVGLLVLVGYALLKKKPHDVFAG
                     FIKATVGYMILNVAAGGLVTTFRPILAALNYKFQIDAAVIDPYFGLAAANGKIAEEFP
                     DFVSAATTALLIGFGVNILLVALRKVTKVRTLFITGHIMVQQAATVSLMVLFLIPALR
                     NQFGTAAIGIICGIYWAVSSNMTVEPTQRLIGGGGFAIGHQQQFAIWFVDKIAPKLGK
                     KEENLDNLKLPSFLNIFHDTVVASATLMLVFFGVILFILGPEIMANPEVITSGAPYNP
                     AKQAFFMYVIQTAFTFSVYLFILMQGVRMFVAELTNAFQGISSKLLPGSFPAVDVAAS
                     YGFGSSNAVLSGFAFGLIGQLITIVLLIVFKSPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAAILSFISGVLQVALGAVCVGLLELSGGYHGNIDFEVPWLPFGFAFKYLGIAGY
                     VLVCLFLLAIPQLQFAKAKDKEAYYRGEAQAE"
BASE COUNT         3376 a         2762 c         2539 g         3530 t
ORIGIN      
        1 aaaatctaac gaaattttcg cgtttgtaat aatcctccaa agtctctccc gactgggtta
       61 ctgtaatcca tttcgggaga atcttagata gtaggtaaag cggaatgagg gcaggatacc
      121 aaatgaacat gacttcaaag atgaaccggt cgttgttgcc tcttgaaatg taacgttggc
      181 ggtattcttc tttgagagaa gggtggggaa tatagatatg aaatggtaca ccaccgcgta
      241 tagacatggc cagcaacatt ctcatcagaa aattcagcgc tactaggact gtctttcctt
      301 gtctatgaag agtgtagccc ttattgacat agattgtttt atagcgttta tccttcagtg
      361 ggcgtttggt ggattttcgc tcctctttcg ttaaatgacg aacactgcta ttatcgtact
      421 taatgtcaga catttctaag tccacaacat ctgtatattt cttagcaagt gtcgtttttc
      481 ccatacctgg aaaggcaaat aaaaacatag gctttcctcc ttattcctca gtagaatagc
      541 agagcatcca aaaagttttc ttaagttgcc cctattttgc ctctcatttc taaaaataag
      601 ccaaagaaaa ccctctgaaa gcctgatttc aaagggttct tagattatac gcaaaaagag
      661 cacacactcg acctcgctta gggctgctgg attcctcccc tgacccgctt cacgcacgaa
      721 tgttgctcca tttaatatta taacacaatt tggccagagt gcaagtttgg aatattaact
      781 agctatccga agttgcttcc tcttgcgtct tcatgataag tttagcagcc ttctgacttt
      841 ctctgcgttg tctgaaaaaa tcttgcatga tattggcaca ttccgcttct aaaatacctg
      901 tttctacctt gacacgatgg ttgagccgct catctgtcag aatgtcatag aggctacctg
      961 cagctccaaa ctttttgttg gttgctccat agatgacctg cggaatgcgt gctaggccaa
     1021 tagctccgct acacatcaca caaggttcaa tcgtcacaaa aagggtcgaa tcaagcaagc
     1081 gccaattccc ctcaacattg ttagcctctt ggattgccat aacctcagcg tgcatgattg
     1141 cctgattgag ttcctcacgc gcattatgcc ctctgccaat aatctggcca tccttgacaa
     1201 tcacacaacc aattggaatt tcatcctttt ctagcgactt tctagcttct tgcaatgctt
     1261 gagtcataaa atactctttt tcttcatgtg tatagttcat aaatctatta tagcacagcc
     1321 ctgataacag ttcaaaatca cttctttatt cgctgctatc aaaatcaatc taacactact
     1381 tcttccttat cacacatttc tttcaaaatt ctgcccttct attaacttaa aaatataaaa
     1441 atacctcctc agtcgagaag gtagggggag caatattgat ttttacagat attaatatat
     1501 catttattgt cgatattgtc aagaaaattt ttaaaaaaat tcaaaaacag cctcgaaagg
     1561 ctgtatctga cattttctat tacaattcgc caactaattt cacaacattt tctactgtga
     1621 agccgtagtt gtcaataact gtttgggctg gagctgaggc accgaatgtg tcgataccaa
     1681 ggactttacc gtcaagaccg acatacttgt accaactttg agttgcaccc atttcaattg
     1741 ccaaacgacg acggatggca tttggaagga tttcttcctt gtaagctgcg tcttgtgcat
     1801 cgaagagttc agttgatggc acagaaacca cacgaacctt agtgcctgct gcttctaatt
     1861 ctttggcagc tttgacagcc aagttcactt ctgaaccaga tgcaagaagg attgtatcaa
     1921 agcctgctgc ttcatagaca acataagccc cttttgcaac cttgtcaaag tctgttcctt
     1981 cttcaactgt caagttttga cgagtgagaa caagggctga tggtgtagac ttgctagtca
     2041 atgacaagta ccaagctgcc tgtgtttcac gcgcatctgc tggacggaaa acgttgaggt
     2101 ttggcatagc acgaagtcct gccaagtgct cgattggttc gtgagttggt ccatcttcac
     2161 caactgcgat agagtcatga gtgaagacat aagtcacagg aagtccttga agagctgaca
     2221 aacgaactgc tgcttttaca tagtctgaga agacgaagaa ggtaccacca tagacacgaa
     2281 gtccgccgtg ggctgccata ccattcaaga tagttcccat ggcaaattca cgcacaccga
     2341 actgaatatt gcggttgagt ggatttactg aatcttgcaa accatcttcc ttgatgtagg
     2401 tcatgtttga gtgagccaag tcagctgaac caccgaggaa ggttggcaag accttagccg
     2461 ctgcgttaag cgcatcttgt gatgagttac gagttgcttg tgagaagcca ttctcataaa
     2521 ctgggaagtc ttctggcttg atttcaacta cgtcacgacc ttccaagata gctgttactt
     2581 cagctgctaa ttctggatga gctactttgt agtcttcaac taatttcacc caagcatcgt
     2641 aggctgctgc accgcgatca gctacattag ctttgaagtc cgcatagact tcagctggaa
     2701 tttcaaatgg accatagttc caatcaagcg ccttacgagt tgcttctgtt tcttctgcac
     2761 caagtggagc accgtgaacg gcatttgtac cttgtttgtt tggcgaacca tgaccgataa
     2821 cagtcttgat ttcgataaga gatggcttac cagctacttt tgccttttca atagcagcaa
     2881 agatggcatc tacatctgta ccgtctgtta caaggtctgt atgccagcca taagcattgt
     2941 aacgggcacg tacatcttct gtgaaggagt cttttgtttc gccatccaag ttgatgtcat
     3001 ttgaatcgta aagaacgatg agtttttcta atttttgaag acctgcgtaa gaagccgctt
     3061 ctgcagaaac accctccatc aagtcgccat caccacagat tacgtaagta tagtggtcaa
     3121 agattgggaa accatcacgg ttgtacttag ctgcaaggaa acgctctgct tgcgcaaaac
     3181 ctgtcgcagt tgagatacct tgtccaagag gacctgttgt tgcatccaca cctgctgtgt
     3241 gaccgaattc tgggtgacct ggtgtttttg agccccactg acggaagttt ttaatctcgt
     3301 ccatagtcac ttcttcaaaa cctgaaaggt gaagaagtcc gtaaagcagc attgacccat
     3361 gacctgctga taagataaag cggtcacggt tcacccagtt tggctgagca gggttgatac
     3421 gaagttcttt agtaaaaagg ctgtaggcca ttggagccgc acccatgacg acacctgggt
     3481 gacctgattt tgatttttcg atggcatcaa taccaaggaa acgaatcgca ttgactgaaa
     3541 gatttgacat gtttttctcc ttttttaaag tcatgaattt attataccat aaagcgtttt
     3601 ctatgtctgt ttgaatgaag cgagaaataa attgtccgta gaaaagaggc aggactttcg
     3661 tcccgccctt ggagtgaaca gtagtaattc gcacatttca tactttatca gagtttatag
     3721 tgcctctcct cgtctctatg gggcaagaga gttctaaatc ttataataaa gccttaaatt
     3781 gaatatttga gtcttctaag aagcaatcat caaagccacg tgggtggtga tattcgatac
     3841 ggtccttgtc catcgggtaa gtatacttgc caccaacttc ccagatgaat ggatggaaat
     3901 cgtattgcaa gcgttctttc cgcattttcc agagctgtag gatttcatct gttgacgcca
     3961 tgaagttaga ccagatgtcg tagtgaacag ggatgattac cttagcacgc aagttttctg
     4021 ccatccggag aaggtcgatt gatgtcatct tgtcttggat accgattggg ttgtcaccgt
     4081 agttgttgat agcaacgtcg atgttgaagt cacgaccgtg ttttgcaaaa tagtttgaga
     4141 agtgggagtc tgcaccatgg tagatagttc cgccaggtgt ttcaaagaca tagttgactg
     4201 ccttgcgagc catgagttca tctgtcacag gaagtcctgc taatttacca ccattttcgt
     4261 cataaccttc gatcggaagt gtcaccaagc aagtacggtc gaaggattct acagcgtgga
     4321 ccttgatgtc tttaaactca aagctatcgc ctggtgcgat acggatgata cggtcagctg
     4381 gaacccccca tttttcccag atatcgccac tttcaaccgg tccgacaaat ttcacatggt
     4441 ccaatttagg attgttgatg atggcagcgg cagtgctgat atccatgtgg tcgctgtgga
     4501 cgtgcgatac tagatagtag tccaattcat tgatggcaaa tggatcgata accattggct
     4561 gcacacgcaa gtttggttgc aatttgcgca cgccagccat gttagccatc tggtgtcccc
     4621 aaaccatatc tttcactttt ttagtggatt ttccacgtga tgaccagagg tccatgacca
     4681 cgttagctcc acctggtgtt ttaatccaga caccacagtt tccaagccac cacatagcaa
     4741 agttgccctc tggcactact tcttcttcga tttcttcatt gagccaagtt ccccactctg
     4801 ggaaggttga aagaatccag gattctcttg taatatcttg aactttagcc attttccatt
     4861 cctcctataa ggtacgtttc tatgcttcta gtatatgctc caaaggcaac aaaaaaaaga
     4921 ccaaataaaa cacaggcttg tgttcaaact tggactttac cctaatacag atctgtttca
     4981 atatcttgac tggataaatt ggttaataat tcattggtaa tcagtttttg tagccgttgt
     5041 ttgagttctc gagcggtcct tccgtctgcg atatatcccg atagaagctt gtctagttct
     5101 actgcaaaaa cttgtttttc atctgccaaa ctacgatatt ttgcaaaatg agtcatgttt
     5161 agcacatcat caaaatctaa aatggggtga acctgcacca aagggaaatc agtttccaac
     5221 ccatcgctgg ttgtaatcaa gaaatcaacc tctagtaagt cttcgacact cttgaattgt
     5281 tcagtggtaa agactgcgcc aatcttctta tctggcaagt gatgctggca ttgtttataa
     5341 agtaagcgtt ggactgacac cccttcatca cagattaaat agacaatctg tgtctgtgcc
     5401 ttgcgtgtcc cactgcgtcg aatagctcct cctagatgaa tcgtcaaata ggcaatatct
     5461 ccatcggata actcaatctg ccactcctcc tccaaaatct ttccagaaac ccgtgtcata
     5521 gcaaacaggg tgctgtattt atcttggatt tgttggacca ttggattttt agataggatg
     5581 ccgtaggttt ttctgaacaa cagagcctta caatgtgcca agagatgttt ggctaattcg
     5641 tcaggatttt ccaattcaaa tggtgaatgt atctcaaaat gatgaataaa tcgctctacc
     5701 actcgcttca agtcaatgaa atcttggctg aatacatgaa catcgctgtc ctttcgatag
     5761 gatagaagta aaatggcaat cagggagatt tctatttcat ccaatttcaa atcaaaggtt
     5821 tgcaacaagg catctttcaa ttcctgagca actcggtact cgatacgctc tcgaacttgt
     5881 tcaaattctt tgagaatggc tcgttcttcc aagtcatcca gctccatatt gcgatagcta
     5941 aggagcaaga aaggcaatac tttgagcata aactcgattt cgtagcgatt gattttcttg
     6001 cccagtttct tttctaactg cgatacttgt ccctgtaaaa attgtgccat ttcttctgag
     6061 aagagcaact gacaccccac caactcaaat aacttagctt ctagaacctg caaaaaacct
     6121 ttattggcat ccaagaagat ggtatagaga agtgagtgga catattggat tttatttaaa
     6181 gggtgacaaa cgagactata gccatgactc ttagacacat agagggtcat cttgtattgt
     6241 tcttgagcca actgggttcg aatttcattc aaatcattta agaccgtatt acgagagact
     6301 tctgtcaact ccattagccg ttcaatggtc actctttctg gtgtgataca gatatatacc
     6361 aacatcagct gaatgcgctc aggcatgctc atgacgtagg tgtacgaatc aatggaatcg
     6421 agaagatctc gacaggctag tttttgcgct tcggtaagtc gaataccgag gcgaggtaag
     6481 ctttcaatac tctctacagt tgttggtagg gcatcattga ttttttctag atggtaatag
     6541 atttttcgac gtgattgttg caagcccttg gaaatagcca tgattgtctc aggttcctgt
     6601 agaccaatca ggtacctcag tagagcgcaa ctactcttat ctaataacaa gattctcctc
     6661 ctctttcccc cgtcccgtta ctatcttaac ataattatct gggatattct aataattatt
     6721 aaaattcatc tgattattac ttgttttaca ttacataaat atttgtagtt agaattaata
     6781 gatgctttta ataatttcta ctcgggtaat cagttctccg tgcatgttat ctatccgaag
     6841 ggcaacattt ttcaaatata cacaggcacc aatagcaatg gttttctctt cttcattgag
     6901 ttttcgtgtc acaaaatctc tcaaagtctc actaccttca cttggaattt ttactcgtgc
     6961 gatgatatta gccactttta ctttctggct ggcgcgcata ataatattat tagaatcatc
     7021 aaactgagtg aagaggtatt ttctaactgc aaataggatg gcgaccgaaa taattccaat
     7081 caggtaatca ctactagaac catgctctac aatcagttgg cgagcaatgg caaccaggag
     7141 tacctcaatc acggtgctag gtgactgttt gcaaagcatt ttgataaatt cgactccgac
     7201 cgccaaggtc attgcacgac tcagaaaatt ttgaataaac gtatccgctt gaatatcacc
     7261 agctaatgag ctgagtaggt ctgcaaaaag aagcgttgac aaggctaaaa gtgctaagcc
     7321 aagtatggca gataaaatca attctaaata gtaggctgtt tctgtcagac tatcctgtaa
     7381 aaatttcctc atctttctcc tcatcaaaag ggaaaaagca gttctccttt tgggggagtc
     7441 tgctactttt ccctactgtt tttttcacac aagaacttaa tgttcatcac ccttttgtcc
     7501 atagtaggca tttggaccat gtttacggag gtagtgcttg tccatgatat agcttggtgc
     7561 tggctccacc cttggattaa tctgctcggt gtagcggttc atccgagcta cttcctcaag
     7621 aacaacgctg tggtagactg cctgagcagg attcttgccc caggtaaatg gtccatggtt
     7681 acggacaaca attcctggta cagcaactgg gtcgatacca cggcgttcaa attcctcgat
     7741 gatgactgaa cctgtttctt tttcataggc cgagtttact tcctcagctg tcaaagaacg
     7801 ggcacatggt accggaccat agaaatagtc cgcatgagtt gtcccataaa atggaatgtc
     7861 acgacctgcc tgagcccaac caactgcttc tgtcgaatgg gtatggacaa cagcgtggac
     7921 atttggccaa gccttgtaga gggcaacgtg ggtcgccaag tcagacgaag gattcaagtc
     7981 cccttccact acatttccgt ccaagtcggt caccaccatg ttttccggtg acaatttttc
     8041 atagtccaca ccagagggct tgatgacgat gcgaccaagt tctcggcaga cttctgatac
     8101 gttgccccag gtaaacttga cgagcccatg agcaggcagg gcgatattgg cttcatagac
     8161 ccgttgacgc atttcctgta aatcttttgg cattagttta accccgcttt ctcaatcaat
     8221 ggatagagga aatcttgtgc ctccttgata gctgctcgtg tttcctcaac cgtttcgcag
     8281 ttttcagacc acatctcaat caagaatgga ccttggtagt tggtcttttt caagactgca
     8341 aacatattgt cccagtccac acagccctga ccaaatggga catcacggaa ttgacccttg
     8401 gagttttcgg ttaccgcata ggtatccttg aggtgaaggg ctgcgataga acggtgtccc
     8461 aggtaaaatt cgctgtaaat gtcattatgc caagcagaca cgtttccagt atctggatag
     8521 acaaagagat aaggcgagtc gatttctttt tcaacggcta ggtatttttc aattgagttg
     8581 ataaatggat catccataat ttcaatgccc aacatgacct gagcctgttc tgcccagtcg
     8641 caagcctgac ggagattctt gatgaaacgc tctctagttt caggtgattt ttcctcgtag
     8701 taaacgtcgt agccagccaa ctggatgaca cgaacgccca aatcctgagc cagttcgata
     8761 cactgtttca tggtttctaa ggacttggct tcaagagcag ggtcattgga gccaagcggg
     8821 tagcgacggt gacctgagaa acaaatggtc ggaatgcgga cacctgtttc atagatggcc
     8881 ttgaccaagt ctaaacgctc ttccttggtc cattccaaac gagccaagcg tgcatcgctc
     8941 tcatctaccg acatttccac aaagtcgaaa cctaattctt tggcaaactc caaacgttcc
     9001 ttccatgtga agtgtttggg cgttgccttt tcatatattc cgattggtcg tgccataggt
     9061 cttaccccca gatacgacgg atttcgtctt taaattcacg cgccgctgct gctgggtcgg
     9121 ctgcttctgt gataccgcga cctgcgataa aggtgaagac atctacaccc tcgaagagtt
     9181 taagggtatc aacgtccaaa ccaccagtta cagaaacacg gaagcccatg tcaaccaatt
     9241 ttttcacttt attgaggtct ttttcgcccc aagtttcgcc agcaaggagg gcgtcacgcg
     9301 attggtggta aatcgcttgg gaaatacctg cgtccaacca gagttgagcc tgttcataag
     9361 tccagtcacc atagagttcg atttggattt cacccttatc accacgcact tccttgatag
     9421 ccttcaaagc agctgccata gttggaatag ttgctgaaca aatacaagtc atccagtccg
     9481 caccacgaac agcattgttt ttagccacag taccacccgc atccgcacac ttggtatctg
     9541 caacgataat tttgtcttgg aagaggctac gtagcacttc taccaattcg ctaccaactt
     9601 gaagcaaaca aacggtacct gcctcgataa catccacttc atgaccaacg gaaacagctg
     9661 ctttaatcgc tccctgcaag tctgaatggt caagggcaac ttgtaaattt ggaatatgtt
     9721 tcatgttttt tctcctaact ttctaaatct aaaccttcta ggtaaggact gtttttggac
     9781 tcttccacca ttgccaagac ttcctcagga gtttgacaag caatcaaacg ctcaatcgaa
     9841 ttttccaatt cgaagagggc gataatttgt ggaatagcta cggatgtatg aatctttgga
     9901 tcggtagctg caagagtgag cagaacagac acttctttcc catctgagaa gacgactggt
     9961 tttgtcaagg taatcaaagc aaaggaatcc ttcaagacgc cgacacctgc ttgggcgtga
    10021 ggcatagcca taccaggcat gagcacatag taaggtccat attcttcagt tgatgcgatg
    10081 atcgcgtcgt agtattcctc agttgcagca ccactttcaa tcagcggttc aacagccttg
    10141 tgaacagcct cctgccaggt ttccgctgtc aatcccaagc gaatggaatt attttctgta
    10201 aatgattttt gtaaattcat agtttttccc ttctacacgc tatatgaaag ggggagggaa
    10261 actccctacc cctaacggct atttatagca ctgcttgtaa ttttgttttg atttcgttgt
    10321 cgtccatgag gttgtcaaga ccaaccaagt gacctttcgt gcgaccttcc aactcagaaa
    10381 tcaagtggtt agaggctacg acaatatcat atcctgccgc taaacctttt gcttcaccga
    10441 ctgagcaaga agctgattga aaatctgtta cgccgagttg acgaagggcg ttttctacct
    10501 tcatcttgat gaccatagat gagcccatgc cgtttccgca ggctgttaaa actttaacca
    10561 taatttcact cctattttat ttttaataca ttatgttctg attttacaga ccaaggatac
    10621 tattctgctt gtgcctcgcc acgatagtac gcttccttat cttttgcttt cgcaaattga
    10681 agttgcggaa tagccaataa gaagaggcag acaagaacat aaccagcaat accgaggtac
    10741 ttaaatgcaa atccaaatgg taaccatggc acttcaaagt caatgtttcc gtggtaacca
    10801 ccgctcaatt caagcaagcc aacgcagact gcaccaagag caacttgaag gacacctgag
    10861 atgaaggaaa ggatggctgc cgctttccaa ccaccacgtt tgtcagcgta cactgcgata
    10921 gctgcgttat cgaagaatac aggtacgaaa ccagtgatga tgaggattgg actcttgaag
    10981 acaatcaaga gaacgattgt aatcaactga ccgattaaac cgaaggcaaa acctgaaaga
    11041 actgcgttag atgaaccaaa gccgtaagaa gctgcaacgt ccaccgctgg gaatgaacca
    11101 ggaagcaatt tgcttgagat accttggaag gcatttgtca attctgctac gaacatgcgg
    11161 acaccttgca tcaaaatgaa gagatatact gagaaggtga aggctgtttg aatcacatac
    11221 ataaagaagg cttgtttagc tgggttgtat ggagcacctg aagtgataac ttctggattt
    11281 gccatgattt ctggacccaa gatgaacaaa atcacaccga agaatacaag catcaatgtc
    11341 gctgaagcaa caactgtatc gtggaagatg ttgaggaagc taggtagctt caagttatcc
    11401 aagttttctt cttttttacc aagttttggt gcaatcttat cgacaaacca gattgcgaat
    11461 tgttgttggt gaccgatggc aaatccaccg ccacctatca atcgttgggt tggctcaact
    11521 gtcatgtttg agctgactgc ccagtagata ccacagatga taccaatcgc tgctgtaccg
    11581 aattggttac gaagagccgg gatgaggaag agcaccatca atgagactgt tgccgcttgt
    11641 tgtaccatga tgtgtccagt gatgaacaga gtacgtactt tggtaacctt acgtagtgcc
    11701 accaagagga tgttgacacc gaaaccaatc aagagcgctg tagtggctgc tgatacgaaa
    11761 tcagggaact cctcagcaat tttcccattt gctgctgcaa gaccgaagta agggtcaata
    11821 actgccgcat caatttggaa cttatagttc aaagccgcca aaatcggacg gaaggttgtg
    11881 accaaaccac cggctgctac gttcaaaatc atatagccga ctgtcgcttt gataaaccct
    11941 gcaaaaacat catgaggctt tttctttaag agggcatagc ccaccaacac taaaagacct
    12001 acgaagaagg ctggattctg caggatattc tgtgagaacc agtttaatgg ggtttgaagg
    12061 aaatccattt ttcattctcc ttttttgaaa tgatttttat ggttttatta taagttatgt
    12121 aagcggaaac aaaaagacca aataaagcac aggcttgtgt tcactattgg actatttccg
    12181 aactttaatg gctgatttaa tcagaat
//
LOCUS       DBNFCK010000057        12159 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.057, whole genome shotgun sequence.
ACCESSION   DBNFCK010000057 DBNFCK010000000
VERSION     DBNFCK010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12159
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.057"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(219..929)
                     /locus_tag="AC1PIW_001198"
     CDS             complement(219..929)
                     /locus_tag="AC1PIW_001198"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIP6606137.1"
                     /translation="MKALIFDVDDTLYDQIQPFERALERHIEVAREQIEPLYLSFRRY
                     ADEVFEATAIGKMSLKDSHIYRMKHALADFGYQVSDATALAIQIDYDYFQGQIELSPV
                     FPEIFSWCQVQGIAMGIITNGPYRHQLRKIRTMGLVNWFELEHVLISGQVGITKPNPA
                     IFQLMEERLGMSGEDICYLGDSFENDIIGAKTAGWQAVWFNHRKRSEPESSFQADYMI
                     DEWFNLDSLIKNIIKPIG"
     gene            complement(983..2722)
                     /locus_tag="AC1PIW_001199"
     CDS             complement(983..2722)
                     /locus_tag="AC1PIW_001199"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6606138.1"
                     /translation="MTILGFLLKQIRRVKCLFIVAVGFYLLASTMVRLAPLLIQQAID
                     GPITDLSKGLPFDEAVFLNQSVQYMGMIILGAIGFYLSMRLLMHCANRIAENLRNQAY
                     DVMQRLPISYFDDKPAGKIATRIVNDTETLRTQFYGTLVNAFNNIVRLLFTYGVLFYM
                     NRSLGWLMLLLIPLYIGIQFAYKKMTDKPMKDFYDARSDVNTQVNETMNGASLIQLFG
                     QEERIMKEFEVTADKMRRADNKIIWAQSLATWNLSGFLQNLVIAAILTVVGYQFLAGQ
                     DGVTAGKLFVYVNYIEGVFIALGALVQQFPNMLRSFETGKRLMALLEEELEDDCDRIL
                     EVEQGQVVFEHVNFSYEENRPVLKDITIRAEKGETVALVGHTGSGKSSIMNLLYRFYD
                     PQEGCVLIDGKNIRDYSRESLRSHMGIVLQDPYLFTGTIASNVSMNEEEADRTRIMQA
                     LEKVGAGPMLSRLEKGIDEPVVEKGAAFSSGERQLIAFARTLYSDPKILILDEATSHI
                     DTETEEIIQHAMEVVKEGRTTFIIAHRLSTIQNADQILVLDQGRIIEHGKHEELVARG
                     GVYAQMHEIQAKV"
     gene            complement(2722..4449)
                     /locus_tag="AC1PIW_001200"
     CDS             complement(2722..4449)
                     /locus_tag="AC1PIW_001200"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6606139.1"
                     /translation="MFRLIFDYIKRHKWLYLLVAVTLIIYDATLLLPTQIIQRMVDIL
                     TKNELTQAILVQEMTLLLLVTVLNYATAFIWHLKLFQASVNFKFDMQQRAFKKLVTMR
                     TPFYEKFRSGDVMTRFSTDVDGLMEMVGYGLMIVVYAGGMLAFIIPTMFFIDWKISLV
                     ALLPMLFMTLCIFFIGRKQDKAIDANREAVAQLNNEVLEVIEGIRVTRAYSKKANQKA
                     QFQARTKQLAQGGDRITSLQSLYNPLATVCLGLSTIFVLLMGAQAVKAGQLTLGQVIA
                     LQLYVGSLLEPFWTLADFILVYQTGKTSFEKLQELIETGDDLEADGSKEIAELSSISF
                     KNYSFSYPQAERASLQDINWTLKAGQTVGIVGKTGSGKTTLVRQFLRQYPIGQGNFFI
                     NHQSILDFKRSSIEEKIGYVPQEHILFSRSVGENIALGKVASSSEEIEQAIATAAFSQ
                     DLKRMSDGLDTMIGERGVSISGGQKQRISIARAFLREPDLLILDDSLSAVDARTERQI
                     IQNIQKERAGKTNVIVTHRLSAVNHADWVLVLDEGRIVEEGRPADLLAQRGWYYEQYQ
                     RQQSQEGGE"
     gene            complement(4919..4991)
                     /locus_tag="AC1PIW_001201"
     tRNA            complement(4919..4991)
                     /locus_tag="AC1PIW_001201"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4956..4958),aa:Thr,seq:ggt)
     gene            5148..5618
                     /locus_tag="AC1PIW_001202"
     CDS             5148..5618
                     /locus_tag="AC1PIW_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HIP6606140.1"
                     /translation="MKLQNLQVGQSYLVKDCLTQDDIRKHLAHLGLKVGEEIRIISKT
                     KTNAIFQVKASRLALDREIIESLVLTEKSATEIINLSEAPIGSSAKVMDIYATGALRR
                     RLMDMGLTKNTQLFLKKVAPLGDPIETTLRGYELTLRKSEAQMIGVQITSEVRK"
     gene            5615..7759
                     /gene="feoB"
                     /locus_tag="AC1PIW_001203"
     CDS             5615..7759
                     /gene="feoB"
                     /locus_tag="AC1PIW_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HIP6606141.1"
                     /translation="MTKHIALAGNPNSGKTTLFNLLTGTNQRVGNWPGVTVERKSGTI
                     KKRKHLLIQDLPGIYSLSPYTPEERVARDYLIADQPAAILNVLDATNLERNLYLTLQL
                     LEMGLPIVIALNMTDALKSQGRSINSDQLSYQLGVPVQPISALKKLGISQLLHEVEKI
                     TNQQAEPIYPQYDKQFEAGLAQVIDLLSDSIPNRQKRFYAIKLLEQDQVILDQLQLNA
                     QDMLDLTEIIAILEKIYADDMEAIIVNQRYQFIEKITELVTKDSDKAFNLSDNIDRLV
                     TNRFLALPIFAAVMWLTYFLSIQTVGTMGTDWVNDVLFGELVPGLIQANLDRFEIAGW
                     LQSLVLDGIIAGCGAILGFVPQIFVLFVCLGILEDIGYMSRVAFVMDRIFRRFGLSGK
                     SFIPMLISTGCGVPGVMASRTIENEQDRKITIMTATFMPCSAKLPIISLVAGAFFPDN
                     PWIAPSAYFVGMAAIVLSGMALKKTKQLGGVASPFIMELPSYHLPKLSTVLRYAFDKA
                     LSFIKRAGTIIFVTNIIIWFSSSYNWSLQMVETDESILASIGHSFSLLFAPLGFGNWR
                     ATVAAITGLLAKETVIATFGILYKLGETTEENPELWGLLQQDYTALSAYSFLVFNLLC
                     APCFAAIGAIHREMGEAKWTWIAIGFQTGLAYASSLVIYQIGLVLIHGQLPTVWTFIA
                     IFLIITAIYSLVKKPTNTLPIVTLKTLEKGEY"
     gene            7759..7911
                     /locus_tag="AC1PIW_001204"
     CDS             7759..7911
                     /locus_tag="AC1PIW_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HIP6606142.1"
                     /translation="MPTLILFLIIVILFSLAIRSLIKGKGSCGDCSCDCTIKQEMNES
                     SHHRHP"
     gene            complement(7964..8893)
                     /gene="ftsX"
                     /locus_tag="AC1PIW_001205"
     CDS             complement(7964..8893)
                     /gene="ftsX"
                     /locus_tag="AC1PIW_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HIP6606143.1"
                     /translation="MSNRFFRHFIESLKSLKRNGWMTIAAISSVAITLTLVGLFASVI
                     LNTAKLASDLEQNVRINVYLRANSTDQAETIVNEAGETVANPDYKKVYNQITALENVQ
                     SVTYSSKDEQLQKLTATLGDTWNLFQGDANPLYDAYIIDTTEPQYVKTVAAEIAKIDG
                     VTEVRDGEVETERIFKLANLVRTWGLAATGLLLFTAVFLISNTIRITIISRSREIQIM
                     RLVGAKNSYIRGPFLWEGAWVGLLGAILPSALVYSFYKMIYTSVNASLASQDLSLISM
                     DVFVPGMIGALFVIGIIIGSLGSVISMNRYLKI"
     gene            complement(8886..9578)
                     /gene="ftsE"
                     /locus_tag="AC1PIW_001206"
     CDS             complement(8886..9578)
                     /gene="ftsE"
                     /locus_tag="AC1PIW_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HIP6606144.1"
                     /translation="MGIIEMKDVSKKYGNGTTALRGVSVNVEAGEFAYIVGPSGAGKS
                     TFIKLLYREEKLDKGSLKVGKFDLAKIKKRDVPLLRRSVGVVFQDYKLLPKKTVFENI
                     AYAMEVIGEKPRNIKKRVMEVLDLVGLKHKIRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPENSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIEDGRVV
                     RDEAEGEYGYDE"
     gene            complement(9721..10816)
                     /gene="prfB"
                     /locus_tag="AC1PIW_001207"
     CDS             complement(join(9721..10743,10745..10816))
                     /gene="prfB"
                     /locus_tag="AC1PIW_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775232.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HIP6606145.1"
                     /translation="MDTAEIRQKIEELGKKLTSFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNLAAQKTSQELNELKNTYGNFHQMLDLYDESEILLDFLAEDESVRDELVEKLVE
                     LDKTMTAYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGEMLLRMYQRYGNAKGFIV
                     ETLDYQAGDEAGIKSVTLSFTGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEIEIRDDEVKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVTQSTVDRT
                     QYGNRDRAMKLLQAKLYQLEQEKKAAEVDSLKGDKKEITWGSQIRSYVFTPYTMVKDH
                     RTGYEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            complement(10851..11978)
                     /gene="queG"
                     /locus_tag="AC1PIW_001208"
     CDS             complement(10851..11978)
                     /gene="queG"
                     /locus_tag="AC1PIW_001208"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HIP6606146.1"
                     /translation="MNIKEEIIKLSQEIGISKIGFTTADDFAYLEKSLRASQEEGRSS
                     GFEHKNIEERIRPKLSLESAKTIISIAVAYPRHLPVKPQKTQYKRGKITPSSWGLDYH
                     YILQDKMERLARGIEKLTDGLEYKAMVDTGALVDTAVARRAGIGFIGKNGLVISKEFG
                     SYMFLGELVTNLDIEPDQPVDYDCGDCNRCVAACPTSCLLGDTTMNARRCLSFQTQDK
                     GMMDLEFRKKIKTVIYGCDICQICCPYNKGISSPPVVEIDPDLAEPELIPFLDLSNGQ
                     FKEKFGMIAGSWRGKNILQRNAIIALANSNDRSAIPKLLEIIDKKQNPVHMATAIWAL
                     GQLVKQPNDEMVSFIAGISSDNDDVIAEQAAFLNMVKDLQM"
     gene            complement(12019..>12159)
                     /locus_tag="AC1PIW_001209"
     CDS             complement(12019..>12159)
                     /locus_tag="AC1PIW_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIP6606147.1"
                     /translation="NGLGGAIGINIMYVLTSIWREKAYVRMNIFCFVLTFFVILITYL
                     AM"
BASE COUNT         3649 a         2866 c         2157 g         3487 t
ORIGIN      
        1 gacatgcgaa gcaagtcact ttcgtggagt acggcaaagc gagttcaaac aatccagtgg
       61 agtgtttgaa gtaggaaata agaaaacgga gtttcctctt gcgtcgaagt aattaaagat
      121 aaactaaatg actatacctc ctctttttga acacaaaaac tcccccgact agcttctaat
      181 cctattaaaa acttgttagg agagttattc cattttattt atccaatcgg ctttatgatg
      241 tttttgataa gggaatccag attgaaccat tcgtcaatca tataatctgc ctgaaaggaa
      301 ctttctggct ctgaccgttt tctgtgatta aaccagaccg cttgccaacc agctgttttt
      361 gccccgatga tgtcattttc aaacgaatcc cctagataac aaatatcttc gcctgacatc
      421 cccaaacgtt cttccattag ttgaaaaata gcaggatttg gctttgtgat accaacttgt
      481 ccagaaataa ggacatgttc tagctcaaac caattaacta accccatcgt gcgtatcttt
      541 ctcaactgat gtctataagg tccattcgta ataatcccca ttgcaattcc ttgcacctga
      601 caccacgaaa atatttctgg aaacactgga ctgagctcta tctgcccttg aaaataatca
      661 taatctattt gaatagctaa ggctgttgca tcagacacct gatacccaaa atctgccaaa
      721 gcatgtttca tccgataaat atgactgtct ttcaaactca tctttccaat tgctgtcgct
      781 tcaaatactt catccgcata tctgcgaaag gaaaggtaga gtggttcaat ttgctccctt
      841 gctacttcaa tgtgtctttc caaagcacgt tcaaacggtt gaatctgatc ataaagcgta
      901 tcatctacat caaaaattaa ggctttcatg agtcaattat agcaaaaaat tccagcaaaa
      961 gcttggaatt tttaactgct tattacacct ttgcttgaat ctcatgcatt tgagcatata
     1021 ctccaccacg tgcaaccaat tcttcatgct tcccatgttc aataattcga ccttgatcta
     1081 agacaagaat ctggtctgca ttttgaatgg tagaaagtcg gtgagcaata ataaaggttg
     1141 tccgaccttc tttcaccact tccatggcat gttggataat ttcttccgtc tccgtatcaa
     1201 tatgagaagt tgcttcatcc aatatcagaa ttttaggatc agagtagagt gtccgcgcaa
     1261 aggcaatcaa ttgacgttcc ccacttgaaa aggccgcccc cttctcaaca accggttcat
     1321 caatcccttt ctctaagcga gatagcatag gccctgcacc aactttttct agcgcctgca
     1381 tgattcttgt tctatctgct tcctcttcat tcatggaaac attgctggca atcgtccctg
     1441 taaagagata gggatcttgc aaaacaatcc ccatgtgact gcgcaggctt tcacgagaat
     1501 aatcacggat gtttttccca tcaatcagta cacatccctc ctgtggatca tagaaacgat
     1561 agaggagatt cataatagaa gacttaccag acccagtatg cccaaccaga gcaactgttt
     1621 ctcctttttc tgctcgaatg gtaatatctt tcaaaactgg tctgttttct tcatagctaa
     1681 agttcacgtg ttcaaacacg acttgccctt gttcaacttc caaaatacga tcacagtcgt
     1741 cctccagctc ttcttctaga agggccatta gacgcttacc tgtttcaaac gagcgaagca
     1801 tatttgggaa ctgttgaacc agcgctccca aagcaatgaa gactccttcg atatagttga
     1861 cataaacaaa gagtttccca gctgtaactc catcctgtcc cgcaagaaat tgatagccga
     1921 ctaccgttaa aatcgcagca atgaccaaat tttgcagaaa accactcaga ttccacgtgg
     1981 caagagactg ggcccagata atcttattat ctgcccgacg catcttgtca gcagtcactt
     2041 caaattcttt catgatgcgc tcttcttgtc caaaaagttg aatcaaactt gcaccattca
     2101 tggtctcatt cacctgcgta ttgacgtcac tccttgcatc ataaaaatcc ttcatcggct
     2161 tgtctgtcat tttcttgtaa gcaaattgaa taccgatata gagtggtatc aagagtaaca
     2221 tcagccaacc aagactccta ttcatataaa ataggacacc gtatgtaaag agcaaacgaa
     2281 caatattgtt aaaggcatta actaaggtcc cataaaattg tgtccgcaga gtttctgtat
     2341 cattgacaat tctagttgca atttttccag cgggcttatc atcaaaataa gaaataggca
     2401 accgttgcat aacatcgtag gcttgattac gtagattttc agcaatacga ttggcgcaat
     2461 gcatgagtaa acgcatagat aagtagaagc caatagctcc cagaataatc attcccatgt
     2521 attgaactga ctggtttaag aaaacagctt catcaaaagg caaacccttg ctcaaatccg
     2581 taatcggccc atcaatcgcc tgctgaatca gcagaggagc caatcgaacc atggtcgaag
     2641 ccaatagata gaagccgaca gctactataa atagacactt gactcgacgg atttgtttta
     2701 ataaaaaacc taaaatagtc attactctcc tccttcctgg ctttgttgtc gttggtattg
     2761 ttcatagtac caacctcttt gggcaagcaa atcagctgga cgcccttcct caacgatacg
     2821 cccctcatcc aagactagta cccagtctgc atggttgaca gcagatagac gatgggtcac
     2881 gatgacattg gttttgcctg cacgttcttt ttgaatattt tggataatct ggcgttctgt
     2941 cctagcatcc actgccgaca aagaatcatc tagaattagc aaatctggct ctcgcaggaa
     3001 agcacgggca atggaaatcc gttgtttctg accaccagaa atagacacac cacgctcacc
     3061 aatcatggta tcaagaccat cactcatccg ttttaaatct tgactaaaag ctgctgtggc
     3121 aatggcttgt tcaatctcct cagagctact agctactttc cctagggcaa tattttctcc
     3181 aacagacctt gaaaacaaaa tgtgttcttg tggaacatag ccaatttttt cctctatact
     3241 agaacgttta aagtctaaaa tagattgatg attgatgaaa aaatttccct gaccaatcgg
     3301 atactggcgt aagaattggc gaaccaaggt cgtttttccc gaaccagttt tcccaacgat
     3361 acctacagtt tgccctgctt tcagtgtcca attgatgtcc tgtaggctgg ctctttccgc
     3421 ctgtggataa ctaaagctat aatttttgaa ggaaatacta gacagctctg caatttcctt
     3481 agaaccatca gcctccaaat catcacccgt ctcaatcagc tcttgcagtt tttcgaaaga
     3541 tgtcttacca gtctgataaa caaggatgaa atctgctagt gtccaaaacg gctctaaaag
     3601 cgaaccgaca tacaattgaa gggcaattac ttgtcccaag gtcagttgcc ctgctttcac
     3661 ggcttgtgcc cccatgagaa gtacgaaaat agtagacaat cccaaacaga ctgtggctaa
     3721 tggattatac aatgattgca aggaagtgat gcgatcccca ccttgagcca attgcttggt
     3781 acgagcttga aattgagcct tttgatttgc ttttttacta taagcccgag tcactcgaat
     3841 gccttcgatg acctccaaaa cttcattatt gagctgggct acagcttctc tattggcatc
     3901 aatcgcctta tcttgttttc tcccgataaa aaatatacat agtgtcatga acagcatcgg
     3961 tagtaaagct actagcgaaa ttttccaatc aataaagaac atcgtgggaa tgataaaagc
     4021 caacataccg cctgcataga cgacaatcat aagaccgtaa cccaccatct ccatcaggcc
     4081 atccacatcc gttgaaaaac gagtcatgac atctcctgag cggaattttt cataaaatgg
     4141 agtccgcatg gtcactaatt ttttaaaggc acgttgctgc atatcaaact tgaaattgac
     4201 agaggcctga aataatttca gatgccaaat gaaggctgtt gcatagttca gcactgttac
     4261 caataacaat aaggtcatct cctgaaccaa tatggcctgt gttaattcat tcttggttag
     4321 tatatcgacc atccgctgaa tgatttgcgt tggtagcaaa agtgtggcgt cataaataat
     4381 caaggtcaca gcaaccaata gatagagcca cttatgacgc ttgatatagt caaaaattaa
     4441 tcgaaacata tatctccttt acaaatccat aacctatcat ttgccacttt attcaataag
     4501 ctacaattga tagaccttac ttttctaagc taagcgcaca aaaaagccca gggaagcatc
     4561 tcactccacc taggcttgcg taagtccaca aaaatagacc agctcttgaa catagtcgac
     4621 aatttttatc acaccatacc cacagtgctt gattgtctca atgtttcaaa atatagccca
     4681 cgaaaaacga caaaagttca tcaatgagct ttgttttcct gcttagaaag gagtgtatga
     4741 taaaaaatag cttgctgaat gacaagttgc ttgttcattt gttttactcc tttcatattt
     4801 ctaagcccta ttctaccata ataaacattc atgtcaaggg attttttgac aataagggcg
     4861 aatttaggac aaaaataaga aaaaaccttg tcaaacaagg cctttcgata gtattcgatg
     4921 ctagttgccg ggattgaacc ggcgacctca tccttaccat ggatgcgctc taccgactga
     4981 gctaaaccag cagtacttga ctagtatatc atatctctaa aaaaagtcaa tggatttttt
     5041 ggaaatattg agaaaaataa agagaaaata agtcattcct aaaaattgtc tgacactttt
     5101 aaggaattgt gctatactat tgttataaaa atcacaaagg aaacgatatg aaactgcaaa
     5161 accttcaagt tggacaatct tatttagtca aagattgtct aacgcaagat gatattcgta
     5221 aacacttagc acacttgggt ttaaaagtcg gagaagagat tcgcatcatt tcaaaaacca
     5281 agaccaatgc aattttccaa gtcaaggcaa gccgtctagc cctagacaga gaaattattg
     5341 aatctctggt tttaacagaa aagtcagcta cggaaataat caatctatcc gaggcaccta
     5401 ttggtagctc agctaaggtt atggacatct atgcaacagg tgcactccgt cgtcgcctca
     5461 tggatatggg acttacaaag aacacgcagc tatttctcaa aaaagtcgct cccctgggcg
     5521 atccaatcga aactaccttg agaggatacg agctgacact acgcaaatcc gaagcgcaaa
     5581 tgattggtgt tcaaatcaca agcgaggtga ggaaatgaca aaacacatcg cccttgcagg
     5641 aaatccaaat agcggaaaaa caactctttt caacctccta acaggaacca accaacgtgt
     5701 cggtaactgg cctggagtca ccgttgaaag aaaatctgga acgatcaaaa agcgaaaaca
     5761 tttactaatt caagatttac caggtatcta ctcgctgtct ccctacacac cagaagaacg
     5821 ggttgccaga gattatttga tagcggacca accagctgct atcctcaacg tccttgatgc
     5881 aacaaacttg gaacggaatc tctatctgac cttgcagtta ttagaaatgg gccttcctat
     5941 tgttatcgcc ctcaatatga cagacgccct gaaaagtcaa ggtcgctcaa taaacagcga
     6001 ccagctctcc tatcaactgg gagttcctgt acagcctatc agtgcactaa aaaagctagg
     6061 tatatcccag ctcctccatg aagtagaaaa aattaccaat cagcaagccg agccaatcta
     6121 tcctcagtat gacaaacaat ttgaagccgg cctcgcccaa gttattgacc tattatcgga
     6181 ttccatccca aatcgtcaaa aacgcttcta cgccattaag ctactcgaac aagaccaagt
     6241 cattctagac caattgcaac taaatgcaca agacatgctt gatttgaccg aaatcattgc
     6301 cattttggaa aaaatttacg cagacgatat ggaagccatt atcgtcaatc aacgctacca
     6361 atttatcgag aaaatcacag aactggtcac aaaagacagc gataaggctt ttaacctgtc
     6421 tgataacata gaccgacttg ttaccaatcg ttttcttgcc ctccctatct ttgcggcagt
     6481 catgtggctg acctacttcc tgtctattca aacggtaggt acaatgggga ctgactgggt
     6541 gaatgatgtc ctcttcggag agcttgttcc aggccttatt caggcaaatc tagatcgatt
     6601 tgaaatcgct ggctggctac aatctctggt ccttgatggc attattgcag gatgcggagc
     6661 cattctaggt tttgtaccac aaattttcgt cctcttcgtc tgcttgggaa tcttggaaga
     6721 catcggctat atgagccgag tcgcctttgt catggatagg atttttagac gttttggtct
     6781 atcaggcaaa tcctttattc ccatgttgat ttccacaggc tgtggcgttc ctggagtcat
     6841 ggcaagccgg actattgaaa atgaacagga ccgaaaaatc accattatga cagcaacctt
     6901 catgccctgt tctgccaaac ttcctatcat ttcactggta gccggtgctt tctttcctga
     6961 caatccttgg attgctccaa gcgcctattt tgtcggtatg gctgccatcg tcctatccgg
     7021 aatggcacta aaaaagacca agcaacttgg tggagtcgca agccccttta tcatggaatt
     7081 accatcttat cacttaccaa aactcagtac ggttctccgg tatgcattcg ataaggcact
     7141 tagttttatc aaacgagctg gtaccattat atttgtgacc aacatcatta tttggttcag
     7201 cagttcctat aattggtctt tacaaatggt agaaacagat gagagtatct tagcctccat
     7261 tggacacagt ttttctctcc tctttgcccc acttggtttc ggaaattggc gagcaactgt
     7321 tgcagccatt acaggtctct tagccaagga aaccgtcatt gccacctttg gtatcctcta
     7381 taaattagga gaaacaaccg aagaaaatcc cgaactatgg gggcttttac aacaagacta
     7441 tacagcgtta tcagcctact ccttcctagt cttcaatctc ctctgtgccc cttgttttgc
     7501 ggctattggt gccattcacc gtgaaatggg agaagccaag tggacatgga ttgctatcgg
     7561 tttccaaacg ggtttagcct atgcaagtag tttggttatc tatcagattg gtcttgtact
     7621 tatccacgga caattaccaa ccgtctggac atttatcgct atctttctaa ttatcacagc
     7681 catctacagc ctagtgaaaa aaccaaccaa cactcttcca atcgtcacgc taaaaacatt
     7741 agaaaaagga gaatattaat gcccacactt atcctctttc tcatcatcgt catcttattt
     7801 tctcttgcga ttcgcagtct catcaaagga aaaggatcct gcggagattg ttcctgcgac
     7861 tgtactatta agcaagagat gaacgaatct tctcaccata gacatccata acaaaaaggt
     7921 ctgcctcatt attgtgcaga cctttttaca tgtagcttgt ctattaaatt tttaagtagc
     7981 ggttcatgga aatgactgat cccaaggaac caataataat accaattaca aaaagtgcgc
     8041 caatcattcc tggaacaaag acatccatac taatcagaga caaatcttga ctagcaagac
     8101 tagcatttac cgaagtataa atcatcttat aaaaagaata gactaaagca gaaggcaaaa
     8161 tagctccgag gagacctacc caggcacctt cccagaggaa tggtccacgg atataactgt
     8221 ttttagcacc aaccagacgc ataatctgaa tttcacggct acgagaaata atggtaattc
     8281 gaatagtatt ggaaatcaag aaaacagcag taaagagcaa taagccagtt gctgccaatc
     8341 cccatgtacg aactagatta gctaatttaa aaatacgttc cgtttcaact tccccatccc
     8401 gtacctctgt aacaccatca attttagcaa tctctgctgc aacagttttt acatattgcg
     8461 gttcagtcgt atcaatgata taagcatcgt aaagtggatt ggcatctcct tggaataggt
     8521 tccaagtatc tcccaaggta gctgttaatt tctgtaactg ttcatcctta cttgaataag
     8581 tgacagattg tacattttct aaggctgtaa tctgattata caccttttta taatctggat
     8641 tagctaccgt ctctcctgct tcgttcacaa tcgtctcagc ttgatcggtt gaattcgctc
     8701 tcaaatacac attgatgcgt acattctgtt ccaaatccga agcgagcttt gctgtgttca
     8761 aaataactga agcaaataga ccaaccaagg taagggtaat ggctacagat gaaatggctg
     8821 caatcgtcat ccaaccattt cgcttcaaac tcttcaatga ctcaataaag tgtctaaaaa
     8881 atcgattact catcgtatcc atattctcct tccgcttcat cacgtactac acgaccatct
     8941 tcaatcgcaa taacacgatg acgaagtgta tttacaatct gactattgtg tgtcgccatc
     9001 aaaatcgttg tcccctgtaa gttaattcgc tccaatagat tcatgatttc ccaagagttt
     9061 tcagggtcca agttaccagt tggttcatca gcaatcaata ctttaggatt atttacaatg
     9121 gcacgggcaa tagcaatacg ctgttgctca ccacctgaaa gttcatttgg gaaagaacga
     9181 attttgtgtt tcaaaccaac caaatctaaa acttccatta cacgtttctt gatattccgt
     9241 ggtttctcac cgataacttc catcgcataa gcaatatttt caaagacagt ctttttaggt
     9301 aagagtttgt agtcttggaa aaccactcct acactacgac gtagaagagg aacatctctc
     9361 tttttaattt ttgcaaggtc aaacttacca acctttagac tccccttatc gagcttttcc
     9421 tcccgataca agagtttgat aaaggttgac ttacctgcac cagaaggacc tacaatgtag
     9481 gcaaattctc ccgcttcaac attgactgat actccgcgaa gggcagtcgt tccgtttcca
     9541 tatttcttgg aaacgtcttt catttctatt attcccatag gatttcctgt cttagacagt
     9601 cctttcttta gatttgagaa cgtttttttc aaaatacagt ccgtattctc taaaaaaact
     9661 tccttgatga gcgaaaaact atctcttatt tacaagcact tcacttgtaa atacaggttc
     9721 ttagctaatt cgccatttta aataagcatc tataaagccg tccaagtctc cgtccataac
     9781 cttatcgact tgagccacct cataaccagt acggtggtct tttaccattg tataaggtgt
     9841 aaagacataa gaacgaatct gacttcccca agtgatctct ttcttgtcac ccttaagtga
     9901 gtctacttct gccgctttct tctcctgctc caattgatac aatttagcct gcaagagttt
     9961 cattgcacgg tctcggtttc catactgagt acggtcaact gtggactgag tcacaatccc
    10021 tgtcggaatg tgcgtcaatc gaacacccgt tgaaacctta ttgacgttct gtccaccagc
    10081 acctcctgac cggaaggtat ccatcttcac ttcatcatct ctgatttcaa tttcaatggt
    10141 atcatccaac tcaggcatta cctccacaga tgtaaaggaa gtatgacgac gcttagctga
    10201 gtcaaaaggt gagatgcgca ccaaacgatg aacacccatt tctgacttaa gcaagccata
    10261 ggcgtttgga ccagtaaagc ttagagtcac agacttaata cctgcctcat caccagcttg
    10321 ataatccaat gtttctacta taaaaccttt ggcatttcca taacgctgat acatccgcag
    10381 gagcatttct ccccagtctt gtgcctcggt accacccgaa cctggatgga tttccaagat
    10441 agcgttattg tgatcatagg gttctgataa gagcaaggtc atttcgtaag cagtcatggt
    10501 tttgtccaat tccactaact tttcaaccaa ctcatcccgc acagactcat cttctgctag
    10561 aaaatctagc aaaatttcag attcatcata taaatccaac atctgatgga aatttccata
    10621 agtatttttt aattcattca actcctgaga cgtcttctgg gctgccaaat tatcattcca
    10681 gaaatctggc tccgtcatct tgttctcaag aatggcaatc tcttcttcca gaccttctaa
    10741 gtcaaagaga ccccctaaaa gaagttaatt ttttgccaag ttcttcaatc ttttggcgaa
    10801 tttctgctgt atccatatac acctcatttt tattcataat ctttattata tcacatttgc
    10861 aagtccttga ccatgttgag aaaggccgct tgctctgcta tgacatcatc attgtctgat
    10921 gaaatacctg cgataaagga aaccatctca tcattgggct gtttgaccaa ttgccccaag
    10981 gcccagatag ccgttgccat gtggaccgga ttttgttttt tatcgatgat ttctaggagt
    11041 ttgggaatgg cagagcgatc attactatta gccaaggcta tgatggcatt acgctggaga
    11101 atatttttcc cccgccaaga acctgcaatc ataccaaatt tttccttgaa ttgtccattt
    11161 gacaagtcca agaatggaat cagctcaggc tcagccaaat cagggtcaat ctccaccaca
    11221 ggtggactgg aaattccctt gttgtaagga caacaaatct ggcaaatatc acaaccatag
    11281 atgaccgtct tgattttctt acgaaactcc aagtccatca tccccttatc ctgggtctga
    11341 aaggacagac agcgacgggc attcatggtc gtgtcaccta atagacaaga cgtcggacag
    11401 gctgcaacac agcgattgca atcaccacaa tcataatcta ctggttgatc tggttcgatg
    11461 tccaggttgg tcactaattc tcctagaaac atataggaac caaattcctt agaaatgact
    11521 agcccattct tcccgataaa gccaattcct gcacgacgag cgaccgctgt atccaccaaa
    11581 gctcctgtat ctaccatagc cttgtattcc aaaccatccg tcagtttctc aattccccgt
    11641 gccaaacgct ccatcttatc ttgcaagata tagtggtaat caagccccca tgaactaggg
    11701 gtaatcttcc cacgtttgta ttgggtcttc tgcggtttga caggcaaatg tcgaggataa
    11761 gctaccgcga tagaaatgat agtttttgca gattctaacg acagtttggg acggatacgt
    11821 tcttcaatat tcttgtgttc aaatcctgac gaacgcccct cttcctgact agcacgaagt
    11881 gatttctcca aataagcaaa atcatcagcc gttgtaaaac caatcttcga gattccgatt
    11941 tcctgagata atttaataat ctcttcctta atattcatta tattcatttt agcaaacttt
    12001 gaatatattt agcaagacct acatggctaa ataagttatt aatataacaa aaaacgttag
    12061 cacaaaacaa aaaatattca tacgaacata ggccttctcc cgccaaatac tggtcaagac
    12121 atacatgata ttaataccga ttgctccccc taagccgtt
//
LOCUS       DBNFCK010000058        11204 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.058, whole genome shotgun sequence.
ACCESSION   DBNFCK010000058 DBNFCK010000000
VERSION     DBNFCK010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11204
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.058"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(783..905)
                     /locus_tag="AC1PIW_001210"
     CDS             complement(783..905)
                     /locus_tag="AC1PIW_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606148.1"
                     /translation="MFALGTIINTLAIALAGLLGSWFGHLLKERHQSGLTMGNV"
     gene            complement(909..1655)
                     /locus_tag="AC1PIW_001211"
     CDS             complement(909..1655)
                     /locus_tag="AC1PIW_001211"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HIP6606149.1"
                     /translation="MQQYFVNGRAPQGMFQISDKDTAKHMFSVMRLQAGDQIVLVFDD
                     GIKRLARVVDSQSQSVEIIEELTDNVELPISVTIAMGFPKGDKLEFVAQKATELGMSA
                     LWAFPADWSVVKWDGKKLAKKAEKLEKIAQGAAEQSKRNRIPAVRLFEKKSDFLAQLA
                     GFDQIILAYEEAAKEGEQANLVKILSGLEIGQSVLVIVGPEGGVSPEEVAAFEGAGAV
                     KTGLGPRILRAETAPLYALSTISYATELLR"
     gene            complement(1657..2610)
                     /gene="prmA"
                     /locus_tag="AC1PIW_001212"
     CDS             complement(1657..2610)
                     /gene="prmA"
                     /locus_tag="AC1PIW_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIP6606150.1"
                     /translation="MNSWQELTIHVHRDAEEAVSNLMIETGSQGVAISDSADYVGQED
                     CFGELYPEVEQSDMIAITAYYPDTVDIEEVKADLATRLADLTGFGLETGQISLESQEL
                     AEEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIRLDPGMAFGTGTHPTTKM
                     SLFALSQVLRGGETVIDVGTGSGVLSIASSLLGAKEIYAYDLDEVAVRVAQENIDLNA
                     NTSNIHVAAGDLLRGIDIKAEVIVANILADILIHLTEDAYRLVKDEGYLIMSGIIADK
                     WDMVRASAEAAGFFLETHMTQGEWNCCVFKKTADRSGVIGG"
     gene            complement(3132..3485)
                     /locus_tag="AC1PIW_001213"
     CDS             complement(3132..3485)
                     /locus_tag="AC1PIW_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HIP6606151.1"
                     /translation="MVHEMLLAPKPFEMMKSGQKTIELRLYDEKRKHIQIGDRIRFYC
                     TENQTQTIEVQVLELHIFDNFAQLYKELDLLSCGYTQSSIREAKPEDMEVYYSREQLE
                     QYGAVGIELRVIDSI"
     gene            complement(3487..3957)
                     /locus_tag="AC1PIW_001214"
     CDS             complement(3487..3957)
                     /locus_tag="AC1PIW_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HIP6606152.1"
                     /translation="MAKHGFLDVLEAALDKHFTYDYELNWDKKNHAIELAFILEAQNA
                     AEIETVDDEGNASSEDIFLEEYVLFYNQDKSRFDEEDYLVALPFDAKKGFSAEFLTYF
                     ASFLKDTADQGLDDLMDFLADPEAQEFAISWDGEAFEKGKADLVEGEFYPYPRY"
     gene            complement(3959..4294)
                     /locus_tag="AC1PIW_001215"
     CDS             complement(3959..4294)
                     /locus_tag="AC1PIW_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HIP6606153.1"
                     /translation="MTELTVSVLEEYLRDHYGTTVPEQSLFMKLVEEIGEVAELLNQR
                     AGRKMMDSEDDSSARLAEELADVIHYAVALAAVNQLDLTKSILEKDERASVKYGREIN
                     LLEFIEKRK"
     gene            4364..5650
                     /locus_tag="AC1PIW_001216"
     CDS             4364..5650
                     /locus_tag="AC1PIW_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HIP6606154.1"
                     /translation="MPANLALRMRPKSIDEVIGQEHLVGPGKIIRRMIDANMLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATTDNQKRLQEIAEEAKFSGGLVLLLDEIHR
                     LNKTKQDFLLPLLENGNIIMIGATTENPFFSILPAIRSRVQIFELQPLQTSHIRQALE
                     LALTDSERGFDFPITIEPEALDFLANATNGDLRAAYNSLELAVLSTKECDDGSRYIDL
                     DAVENSLQKSYISMDKNGDAHYDILSALQKSIRGSDVNASLHYAARLIEAEDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALEAAQKIGFPEARILIANVVVDLALSPKSNSAYL
                     AMDAALADLRKNGHLPIPNHLRDGHYAGSKELGNAIGYQYPHAYPEKWVDQQYLPDKL
                     LHADYFTANDTGKYERALGMTQEKIKNLKKNRRQNP"
     gene            5752..5945
                     /gene="ssrS"
                     /locus_tag="AC1PIW_001217"
     ncRNA           5752..5945
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="AC1PIW_001217"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            5954..6026
                     /locus_tag="AC1PIW_001218"
     tRNA            5954..6026
                     /locus_tag="AC1PIW_001218"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5987..5989,aa:Lys,seq:ctt)
     gene            complement(6132..6689)
                     /gene="nrdG"
                     /locus_tag="AC1PIW_001219"
     CDS             complement(6132..6689)
                     /gene="nrdG"
                     /locus_tag="AC1PIW_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HIP6606155.1"
                     /translation="MNNPKPQEWKSEELSQGRIMDYKAFNFVDGEGVRCSLYVSGCLF
                     HCEGCYNVATWSFKAGIPYTKELEDRILADLAQPYVQGLTLLGGEPFLNTGTVLPLVK
                     RIRKELPEKDIWSWTGYTWEEMMLETPDKLELLSYLDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLQEKRVVLWDKLKK"
     gene            complement(6876..7370)
                     /locus_tag="AC1PIW_001220"
     CDS             complement(6876..7370)
                     /locus_tag="AC1PIW_001220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6606156.1"
                     /translation="MELRRPTIADKETILEMLAEFEKAGSAMDGGFISKDVDFEEWIF
                     RNKDYETGLNLPDGFVPSIQYVSFDQTGRALGFLSLRLRLNDFLLNKGGHIGYSIRPT
                     ERGKGYAKDQLRLGLQEAATKNISKVLVTCLTDNQASRCTIVACGGVLEDVRDGVERY
                     WIED"
     gene            complement(7372..8718)
                     /locus_tag="AC1PIW_001221"
     CDS             complement(7372..8718)
                     /locus_tag="AC1PIW_001221"
                     /EC_number="3.1.-.-"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HIP6606157.1"
                     /translation="MDYTDISILHTNDMHSYMENFPKKAQLIADIRARNEQKGIPTFV
                     FDSGDLFSGNIFFNMYRGVKEIELMNQIGCQAMTLGNHEFDHGDELLSRLDDYAQFPI
                     VSSNLRYRDVEVADELVLLEDVLEFDMNGNPLYVLGLTTLETQEVASPSENVIFEGHK
                     QALRRVVDQVLAKDPQAHMVLLSHLGYDEDVHLAKDFPELNVILGGHTHTILKEPTQV
                     GSVSICQAGQYGRFIGHLSLRCYADGRHEVLAYDLIEVEKLTQEDRAVKAIIDQMKSE
                     RDAVFSQPIAILPQALDGERDSIRQGLSTLAPVICQALFEQASQLGLQVDGAVINGFG
                     IRASLSAGPIFYSDLVKVLPFSKRVLLVRIKGRDLLSSIQTGLHPQMWGIVPGSEGFV
                     VNGRAAESDKDYQIVTNSFVWSGKDNYDDFHKAEIVQDLGLDIDIISEFFKRQYGD"
     gene            complement(8720..8854)
                     /locus_tag="AC1PIW_001222"
     CDS             complement(8720..8854)
                     /locus_tag="AC1PIW_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606158.1"
                     /translation="MGKYQLDDKGKALVTRYHEKNSNVKQDKKARVQELLKQAKNKKK
                     "
     gene            complement(8953..11124)
                     /gene="nrdD"
                     /locus_tag="AC1PIW_001223"
     CDS             complement(8953..11124)
                     /gene="nrdD"
                     /locus_tag="AC1PIW_001223"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HIP6606159.1"
                     /translation="MNVQENVLPSIELLVLKRDGRTVSFDQDKIFSALQRANQELEHP
                     VSEAGLKIVLEAVLAEIGRRFEKDVQIYEIQTVVEQELLKAHLYDLAELYIQYRTRRD
                     FRRHQATDINFEIHKLLSKDQSVVNENANKDADVFNTQRDLTAGIVGKSIGLKLLPAH
                     VANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLAQGFKIGNADVESPKSIQTATAQ
                     ISQIIANVASSQYGGCSADRIDEVLAPYAELNYQKHLKDAEDWVLADKQEEYARAKTQ
                     KDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSWFEREIQKAILNIRIKGLGREG
                     RTAIFPKLIFTVKRGLNLESDSPNYDIKQLAIECATKRMYPDMLSYDKIVELTGSFKV
                     PMGCRSFLQGWKDENGQDVTSGRMNLGVVTVNLPRIAMESAGDKDKFWEIFAERMAIA
                     KDALVYRVERVKEATPANAPILYQYGAFGKRLAKTDAVDEVFKNRRATVSLGYIGLYE
                     VATVFYGGEWETNPEAKDFTVDIIKKMKALVEDWSDEYGYHFSVYSTPSESLTDRFCR
                     MDTEKFGIVKDITDKEYYTNSFHYDVRKNPTPFEKLDFEKIYPAVGASGGFIHYCEYP
                     VLQQNPKALEAVWDYAYDRVGYLGTNTPIDHCYACDFEGDFEPTERGFKCPNCGNSDP
                     KSVDVVKRTCGYLGNPQARPMVKGRHKEISARVKHMNGSSL"
BASE COUNT         3048 a         2753 c         2204 g         3199 t
ORIGIN      
        1 actaattttg ttcgttttta tatacaaatt tttaattttt ctcaaaatca ttaatagact
       61 aattttagat tcgattgaat aaaagatgta gcttaaatat ttcggcgacg ctatttctgt
      121 tgccaaaaac tcggatttgt gagctattat atataagtct gtcctaaatt gtttttcttg
      181 ataaagaatt cccgaactac ttaaaaagga ctttttacct ttttagccta atatgcatga
      241 agaaaaaatt actcacaggc aatctagcac aaataatagc gcagctaggt aaaaagtaca
      301 acgccacttc tttaagttag tgtcaatatc tcagtgcagt agttgattgc tctagcaatc
      361 tgagagaatt gcttgaggtt agaaattgaa caaacaaagt ttgtgccgag acagcccaag
      421 aaactgtttc agcctgaccc tagaaatcaa aagtgaggta aaaaagttgg atataggatt
      481 tgtaaaaaaa atcacagcaa ttcgtatttt atagtctttt aatttacttt tagtacgagg
      541 caatgagccg caggcataac tgaagttagg caaggcgagt taacgaagta ataaaagaaa
      601 aactaaatga cgatacactc aaaatctacc ctaatcagct atgtaaggat gagaagacgg
      661 actctactag gaatagtcgt cttctcatcc ttgcctggtt cttaattcca ttattgaaac
      721 caatcaccat ttaactatct ataccccaat aatcaaagga ttctaacagg ttcaagaaag
      781 actcaaacat tccccatcgt cagtcccgac tgatgccgtt ctttcagtaa atggccaaac
      841 caagatccta gcaaacctgc tagggcaatg gccaaggtat ttataatcgt ccctaaggca
      901 aacatggact acctcaacaa ttccgtcgca taactaatgg tcgaaagagc ataaagcgga
      961 gctgtttccg cccggagaat gcgaggacct aggcctgtct tgaccgctcc tgctccttcg
     1021 aaagcagcta cttcctcagg cgacacgcca ccttctggac cgacgatgac aaggactgat
     1081 tggccgattt ctaagccaga caagattttc actagattgg cttgttcacc ttcttttgca
     1141 gcctcttcgt aggctaagat aatctggtca aagcctgcta gttgagctag gaaatccgat
     1201 tttttctcaa acaagcgaac agccggaatg cgattgcgtt tgctttgctc ggctgcgcct
     1261 tgggcaattt tttctaactt ttctgctttt tttgccagtt ttttaccgtc ccatttgacc
     1321 actgaccagt ccgctggaaa ggcccagagg gctgacatgc ctaattccgt tgccttttgg
     1381 gcgacaaatt cgagcttatc tccttttgga aagcccatgg caatggtcac ggaaatgggt
     1441 aattcgacat tatctgtcag ttcttcgatg atttcaacag actggctctg gctatccacc
     1501 acacgcgcca agcgtttaat accgtcgtca aataccaaaa caatttggtc acctgcctgc
     1561 aagcgcatga cagaaaacat gtgcttggca gtgtccttat cgctaatctg gaacatgccc
     1621 tgcggtgctc tgccattgac aaaatactgc tgcatcctag cctccaatca cacctgagcg
     1681 gtcagccgtc ttcttaaaga cgcagcaatt ccactcgccc tgggtcatgt gagtctcaag
     1741 gaagaagcca gccgcctccg cagatgctcg caccatgtcc cacttatccg cgatgatgcc
     1801 gctcataatc aggtagccct cgtccttgac cagacggtag gcgtcctcgg tcagatggat
     1861 gagaatatcc gccaagatgt tggcaacgat gacttctgct ttaatgtcaa tgccacgaag
     1921 caaatcgccc gctgcgacat gaatattgct agtattggcg ttgaggtcga tattttcctg
     1981 ggctacacgc accgccacct catccaagtc ataggcataa atctccttgg cacctaggag
     2041 ggagctagcg atggaaagga cacctgaacc tgtgccgaca tcaatgactg tctcgccacc
     2101 acgcaagacc tgagaaagag caaagaggct catcttggtc gtcgggtggg ttcctgtacc
     2161 gaaagccatg cctggatcca agcggataat cttctcccca gctgtcgcct catagtccgt
     2221 ccaagacggc acaatggtca aatcgtgggt aatgcgagct ggctcatagt atttcttcca
     2281 gttgtccgcc cagtcttcct ctgccaattc ttgactttct agactgatct gacctgtttc
     2341 caagccaaag cccgtcaaat ccgccaagcg agtcgccaag tctgccttaa cttcctcaat
     2401 atccacagta tcaggatagt aagccgtaat cgcaatcata tccgactgct ccacctcggg
     2461 atagagttcg ccgaaacagt cttcctgtcc cacatagtca gccgagtcgc taatggccac
     2521 cccctgactg cccgtttcaa tcatgagatt tgaaactgct tcttctgcat cacgatgcac
     2581 gtgaatcgtt aattcttgcc atgagttcat gtttgttttc cttttctttt ctatcgtcaa
     2641 tcactgtatt attgtaccga tttctagccc attttacaag ttaatattac gcgatggagc
     2701 cctaataata ctttttcgaa aattttctaa tcgcactgcg aataaacatg tgtaggaaag
     2761 tttgaaacgg tggttccaag tttctgaacg gaggtgatgc ccatgaaagt ggacagcaaa
     2821 tctgctaggg cacgcactag ctcgagtttt gaaaaatatc gtttcaaaac tctcacgtcg
     2881 gaaatctctg gatttatatt tatcaggtct gcttgttgaa aggctgagct tgataatcgt
     2941 catcatttta gctattcgag ataaaaaata accgtcagca aactttgaca cagttctgac
     3001 ggttatttaa tctaacgaac tgaaccaccg tttagcggtc ctactgggcg ttgagctgga
     3061 actcaacgcc ttttctattt ttataatagc aaaaatttcg cctgcagtca agcaagagag
     3121 aaaacagaat gtcaaataga atctattacc ctcaattcta tacccactgc accatactgt
     3181 tctaattgtt ctcgcgaata ataaacttcc atatcttctg gtttcgcctc tctgatactg
     3241 ctttgcgtat acccacaaga caataaatcc agttctttgt ataactgagc aaaattatca
     3301 aatatgtgaa gctctaatac ttgcacctcg atcgtttgcg tctgattttc tgtacaataa
     3361 aaacggatgc gatctccaat ttgtatatgc ttgcgcttct cgtcataaag tcgtagctca
     3421 atggtcttct gaccagactt catcatttca aaaggttttg gagccagtaa catttcatga
     3481 accatctcaa tacctcggat atgggtaaaa ttccccttct accaagtctg ctttgccttt
     3541 ttcaaaagcc tctccatccc aagaaatcgc aaattcttgg gcttctgggt ccgccaaaaa
     3601 gtccatgaga tcatctaggc cttggtctgc ggtgtccttg agaaagcttg cgaaataggt
     3661 cagaaattct gctgaaaagc ccttcttggc gtcaaatggc agagcgacca gatagtcttc
     3721 ctcgtcaaag cgagacttgt cctggttgta gaaaaggaca tactcctcta ggaaaatatc
     3781 ctcagaagac gcattgcctt cgtcgtccac cgtttcaatc tcagccgcat tttgtgcttc
     3841 caagataaaa gccaactcaa tagcatgatt tttcttgtcc cagttgagct cgtaatcata
     3901 agtaaaatgt ttgtccagtg ccgcttccag cacatctaaa aagccatgtt ttgccattct
     3961 atttcctctt ttctataaat tccaataaat ttatttctcg accgtatttg acggaagccc
     4021 gctcatcttt ctccaaaatg gacttggtta aatctagttg atttaccgct gctaaggcta
     4081 cagcatagtg aataacatct gctaattctt ctgccaaacg agcagagctg tcgtcttcgc
     4141 tatccatcat cttgcggcct gcacgttgat tcagtagctc tgctacttca ccgatttctt
     4201 ccactaattt cataaagaga ctttgctctg gaactgtcgt cccatagtgg tcacgcaagt
     4261 attcttccaa aacactcact gtcaactccg tcatcttcgt ttctctccaa atcctaaaat
     4321 tgtagtataa tgattatacc ataaatgcac tgaaaggaag cctatgccag ccaatctcgc
     4381 ccttcgtatg cgacccaaat ccattgatga ggtaatcggt caggaacacc tggtcggtcc
     4441 tggaaagatt atccgtcgca tgatcgatgc caatatgctg tcgtccatga ttctctacgg
     4501 tccgccaggg attggcaaga cctctatcgc ctctgccatt gctggcacaa ccaagtatgc
     4561 ctttcggacc tttaatgcca cgaccgacaa ccaaaaacgc ctgcaggaaa tcgctgaaga
     4621 ggctaagttt tctggcggtc tggttctcct gctcgatgaa atccaccgcc ttaacaagac
     4681 caagcaggat ttcctgctcc ctcttttgga aaatggcaat atcatcatga ttggagcaac
     4741 gacggaaaat ccatttttct ctattttacc cgccattcgc agtcgagtgc agatttttga
     4801 attacaacct ttgcaaacca gccatatccg acaggccttg gagttggctt tgacagacag
     4861 cgaacgtggt tttgacttcc ctattaccat tgagcctgag gctctggatt ttctggcaaa
     4921 tgcgaccaac ggtgaccttc gtgccgccta caattcgcta gaactagctg tactttcgac
     4981 caaggaatgt gacgacggta gccgctacat tgatctggac gccgtggaaa atagcctgca
     5041 aaagtcctac atcagcatgg acaagaacgg cgatgcccac tacgacatcc tctccgccct
     5101 gcaaaaatcc attcggggta gcgatgtcaa tgccagcctc cactacgccg ctcgtttgat
     5161 tgaggccgaa gatctgccta gtctggctcg tcgcttgacg gtcattgcct acgaagacat
     5221 cggcttggcc aatccagagg ctcagattca tacggtgacg gcccttgaag ccgcccagaa
     5281 aattggcttt ccagaagcac ggattttgat tgccaatgtg gtagtcgatt tggctctttc
     5341 tcccaagtcc aattctgcct atctggctat ggatgcagct ctggctgatt tgcgaaagaa
     5401 cggtcatctg cccattccaa atcacctgcg agacggtcat tatgctggta gtaaggagct
     5461 gggaaatgcc attggttacc agtatcccca tgcctatcct gagaaatggg tggaccaaca
     5521 atacctgcct gataagttac ttcatgcgga ctacttcact gccaatgaca ccggcaaata
     5581 tgagcgtgcc ttgggcatga cacaagaaaa gataaaaaat ttgaaaaaaa atagacgcca
     5641 aaatccttga tagaactggt tttttggcac atacattttg acaccgctta catttttgcc
     5701 atttttctct tgatttttca aaaaaaagtg gtattattaa atcataaata agaactgctg
     5761 tggtatacga attcatacct atgtgttgac cgactatttt tgtattacta gggagacaaa
     5821 gatcctctaa cagtatgcaa gctggcacac ccagaagcaa ctaagttaga aacgcgtcac
     5881 ccacctgctt ttagcgtgcg ggatcaatac aatccatgga taaccggtac caatacagca
     5941 tttttatttt ttaccttcct tagctcagct ggcagagcag cggactctta atccgtgggt
     6001 cgtaggttcg atccctacag ggaggattgg ttgatatgag gaaagtcttg atttatcaag
     6061 gctttttctc gtttttttga gtgaatttgg tcaaaatttg gtcaaaaaga accttttcac
     6121 attgtgaagg gctatttttt taacttatcc cacaggacaa ctcgtttttc ctgtaatgat
     6181 ttctgtacat cgatgatgcg ttgattcgat gagcctcgaa attgcagcat aagattgcgc
     6241 ttggtacggt cataacggcc gtcaactaga atatctaaat aggatagtaa ctctaactta
     6301 tccggagttt ctaacatcat ttcttcccat gtgtagcctg tccaagacca aatgtccttt
     6361 tctggcagtt ctttccgaat tcgctttacg agtgggagga ccgtgcccgt gttcagaaaa
     6421 ggctcgccac ccaagagagt taggccctgt acatagggct gggccaagtc tgctaaaatg
     6481 cggtcttcaa gctccttggt atagggaatc ccagctttaa aagaccaggt tgcaacattg
     6541 tagcagccct cacagtgaaa cagacagcca ctaacatata gagagcaacg gactccttct
     6601 ccatccacaa agttaaaggc tttgtagtcc attattcgcc cttggctaag ttcctcgctc
     6661 ttccattctt gtggtttagg attattcatt tcaaatacct gagcataagc aaatgaaatc
     6721 tgatcgtttg gatgaattcc gttttaccag tacacagact gtttttatcc ataaatggta
     6781 cttccaatgc tttagctcgg attgatccca gcaagcttag tgcggaacca atctcaattc
     6841 ctttcctgtt ttgattcagc agtttgtcac tcccattaat cctcaatcca gtatctttct
     6901 actccgtctc gaacatcttc caaaacgcca ccacaagcca caatcgtgca acggctggct
     6961 tgattgtctg tcaagcaagt cacaaggacc ttggaaatat ttttggttgc ggcttcctgc
     7021 aaaccaagtc gcagctggtc cttggcatag ccctttcctc gctcggttgg gcgaatggaa
     7081 tatccgatat gaccgccttt attgagcagg aaatcgttga gccgtaggcg tagactgaga
     7141 aaaccaaggg ctcgaccagt ctggtcaaat gacacatatt gaatggaagg aacaaaaccg
     7201 tctggcagat tgagacctgt ctcatagtct ttgtttctaa aaatccattc ttcaaagtcg
     7261 acatctttag agataaagcc tccgtccata gctgagcctg ctttttcaaa ttctgccagc
     7321 atctccaaaa tcgtttcctt atccgctata gtcggtcttc ttaattccat tttagtctcc
     7381 atattgtctt ttgaaaaatt cactgatgat gtctatatcc agtcccaagt cctgcacaat
     7441 ttctgccttg tggaaatcgt catagttgtc cttaccgctc caaacaaaag aattggtcac
     7501 aatctgatag tccttatctg actcggctgc tctgccattg accacaaaac cctcactgcc
     7561 cgggacaatg ccccacatct gagggtggag acctgtctgt atactggaga gcaagtctct
     7621 gcccttgata cgaactaaga gtactcgctt ggaaaagggc aagaccttga ccaagtccga
     7681 ataaaagatt ggtcccgccg acaaggaagc ccgaataccg aagccgttga tgaccgctcc
     7741 atccacctgc aaacctagtt gactcgcctg ttcaaataaa gcctgacaaa taacaggggc
     7801 cagagttgat aacccttgac gaatgctgtc tcgctctccg tctagtgcct gaggtaaaat
     7861 cgctatcggt tgggaaaaaa ctgcgtcacg ttctgatttc atctggtcga taatagcctt
     7921 gaccgccctg tcttcttggg tcagtttctc cacctcaatc aagtcataag ccaggacctc
     7981 gtgccgtccg tctgcataac accgcaggga tagatgaccg ataaatctgc catattgccc
     8041 agcctggcaa atactgacgc tccctacttg agttggctct ttcaagattg tatgagtatg
     8101 cccacctaaa atcacgttga gttcagggaa atccttggcc aaatgcacat cctcatcgta
     8161 tcctagatga ctgagtaaga ccatatgagc ctgtggatcc ttggcaagaa cctgatccac
     8221 cactcttcgt agagcttgtt tatggccttc aaaaatcaca ttctccgacg gtgacgcaac
     8281 ctcctgggtt tccaaggtag tcaaaccgag gacatagagg gggttcccat tcatatcaaa
     8341 ttcaaggaca tcctcaagca gcaccaattc atcagccacc tccacatccc gatagcggag
     8401 attggaggac acaattggaa actgagcata gtcatccaag cgactaagca actcatctcc
     8461 gtggtcaaac tcatgattgc ccaaggtcat agcctgacaa ccaatttgat tcatcagctc
     8521 gatttccttg actcctcggt acatattgaa aaagatatta cccgaaaaga gatcgccgct
     8581 gtcaaagaca aaggtcggaa tacctttttg ctcatttcga gctcgaatat ccgcaatcag
     8641 ctgggctttc tttggaaaat tctccatgta ggaatgcata tcattggtat ggagaatact
     8701 aatatctgta tagtccatac tattttttct tgttttttgc ttgtttgagc aattcctgca
     8761 cacgcgcttt tttatcctgc ttcacattag aattcttctc atgatagcgt gtcaccaggg
     8821 ccttgccctt atcgtctaat tgatattttc ccattctttt cacctcattt tcatttttga
     8881 acctgtgttc atttataaca aacttccaca gctacctgga actgtggaag tcctatcttg
     8941 tttatttatg atttacaaac ttgacccgtt catgtgtttc acacgcgctg agatttcctt
     9001 gtgacggcct tttaccatcg gacgggcctg cggatttcct agatatccac aagttcgttt
     9061 aacaacatcc accgacttag gatcgctatt cccgcaattt gggcacttga aaccacgctc
     9121 tgttggctca aaatcacctt caaaatcgca ggcgtaacaa tggtcaatcg gagtattggt
     9181 tcccaagtaa ccaacacggt cataggcata gtcccaaaca gcttccaaag cctttggatt
     9241 ttgttgcagt acagggtatt cacagtaatg gataaatcca ccactagcac ctacagcagg
     9301 ataaattttc tcaaaatcca gtttttcaaa cggtgttgga tttttgcgta catcatagtg
     9361 gaaactatta gtgtaatact ctttatccgt aatgtcctta acgatgccaa atttctctgt
     9421 atccatacgg cagaagcggt ctgtcaggct ttcagatggt gtcgaataaa ctgaaaaatg
     9481 ataaccatat tcatccgacc aatcttcaac caaggctttc attttcttga taatatccac
     9541 cgtgaaatct tttgcctctg ggttggtctc ccattcgcca ccgtaaaata cggttgcgac
     9601 ttcatacaaa ccaatgtacc ccaaagatac ggttgctcga cgatttttaa agacttcatc
     9661 tactgcatct gtcttagcca aacgtttgcc gaatgcccca tattgataga gaattggagc
     9721 attggccggg gttgcctcct taacccgctc cacgcggtaa accaaggcat ccttggcaat
     9781 ggccatacgt tctgcaaaga tttcccaaaa cttatcctta tctccagcag actccatggc
     9841 aatccgtggc aaattgacgg ttacgacacc caagttcatc cgaccagatg tcacatcctg
     9901 tccattttca tccttccaac cttgaaggaa agagcggcaa cccattggca ccttgaagga
     9961 acctgtcaac tcaacaatct tgtcataaga aagcatatct ggatacatac gcttggttgc
    10021 acattcaatc gccaattgct tgatgtcata gtttggagaa tctgactcca agttcaagcc
    10081 acgcttaact gtgaaaatca acttaggaaa gatagctgtc cgcccttcgc gccccagacc
    10141 cttgatacga atgttcaaaa tcgctttttg aatttctcgc tcaaaccatg atgtccctag
    10201 gccaaaaccg agtgaggtga agggtgtttg tccattggaa gtaaaaaggg tgttgatttc
    10261 atactccaaa gactgcatag cgtcatagat gtctttttgc gttttagcac gcgcatattc
    10321 ttcttgcttg tctgctaaca cccaatcctc ggcgtctttc aaatgctttt ggtagttaag
    10381 ttccgcatag ggcgccaaaa cttcatcgat acggtcagct gagcagccac catattgact
    10441 tgaggccaca ttagcaatga tttgtgaaat ctgagccgta gcagtttgaa ttgattttgg
    10501 actttctaca tcggcatttc ctatcttgaa gccctgtgcc aacatccctt taaaatcaat
    10561 caaacaacag ttggtcattg gtgtataagg gctgtaatcc aaatcatggt aatgaatatc
    10621 gcctttttgg tgggcattgg ccacatgagc tggtaataat ttgagaccga tagactttcc
    10681 gacaatacct gctgtcaggt cccgttgcgt attgaacaca tccgcatcct tattagcgtt
    10741 ttcattgacc acagactggt ccttgctcaa cagcttatga atttcaaaat taatatcggt
    10801 agcctgatga cggcgaaaat cacggcgtgt tcggtattga atataaagct ccgccaaatc
    10861 atataaatga gcctttaaca attcctgttc aacgaccgtt tgaatttcgt aaatttgaac
    10921 atctttctca aatcggcgac caatttcagc caaaacagct tctaatacaa tttttaaacc
    10981 tgcctctgaa acaggatgtt ctaattcttg gtttgcccgc tgaagagcag aaaaaatctt
    11041 atcctggtca aaggatactg tccgtccatc acgtttcaaa accaataatt ctatactcgg
    11101 taagacattt tcttgcacat tcatctgaca aacctccaaa attcttcact tctattagtg
    11161 tagcacttca caaaacaaaa atcaatatct tgtgtaaaaa aata
//
LOCUS       DBNFCK010000059        11187 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.059, whole genome shotgun sequence.
ACCESSION   DBNFCK010000059 DBNFCK010000000
VERSION     DBNFCK010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11187
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.059"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            255..395
                     /locus_tag="AC1PIW_001224"
     CDS             255..395
                     /locus_tag="AC1PIW_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606160.1"
                     /translation="MIGHAINGIEGAMFAMLYEADRTYYRCQHCGYRFSRKENRFISN
                     IE"
     gene            complement(763..1314)
                     /locus_tag="AC1PIW_001225"
     CDS             complement(763..1314)
                     /locus_tag="AC1PIW_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606161.1"
                     /translation="MNDCDLKDFVGKNFADELPDDDSKIMIHFHTMILELGSIIAALE
                     IVKIVNDEWHDRVVQSSIRYDIVRNVTYESLFYRVVFGITKIFDVREKNGIFKILSKL
                     RHSTKDRSLLSILSTIQEGIDKEQKNIDEIKLLRDKLLAHLDKEMVFSTERLDIGILY
                     YYFEAIEIKSIYTACIELYNAFI"
     gene            complement(1650..2543)
                     /locus_tag="AC1PIW_001226"
     CDS             complement(1650..2543)
                     /locus_tag="AC1PIW_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HIP6606162.1"
                     /translation="MLELKNENLTVQFSELGGQIISIKDKDGIEYLWQGEPTYWSGQA
                     PVLFPICGSLRNDWAIYEPAERPTFTGTIPRHGLVRKMLFKNVKSTENSLEFSISSNE
                     ETVKNYPFEFELSINYSLFGKTIRIEYRVKNLEGHRKLPYFIGGHPGFNCPLLDGESY
                     EDYYLEFEKVESCTVPRSFPETGLLDLRDRRPFLENQKTLDLSYKLFEYDAITLDQLA
                     SRKVTLKSKNHQKKLSIAFDDFPYLVIWSTTNQSPFIALEPWSGLSTSLEESDIFNAK
                     RNISYVAPKEVDTKYFDIIIE"
     gene            complement(2608..4014)
                     /gene="lacG"
                     /locus_tag="AC1PIW_001227"
     CDS             complement(2608..4014)
                     /gene="lacG"
                     /locus_tag="AC1PIW_001227"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HIP6606163.1"
                     /translation="MVKTLPKDFIFGGATAAYQAEGATKTDGKGRVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLELSEQFGVNGIRISIAWSRIFPTGFGEVNPKGVEYYHKLFAE
                     CHKRHVEPFVTLHHFDTPETLHSNGDFLNRENIEHFVNYAAFCFKEFPEVSYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHAKAVKLYKDAGYSGEIGVVHAL
                     PTKYPYDPTNPDDVRAAELEDIIHNKFILDATYLGYYSEKTLEGVRHILKVNGGELDL
                     RDEDFAALDAAKDLNDFLGINYYMSDWMRAHDGETEIIHNGKGEKGSSKYQIKGVGRR
                     ESPVDIPKTDWDWIIYPQGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDGTVYDD
                     GRIDYVKKHLEVISDAISDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAYWYKKVAETQVIE"
     gene            complement(4259..5938)
                     /locus_tag="AC1PIW_001228"
     CDS             complement(4259..5938)
                     /locus_tag="AC1PIW_001228"
                     /EC_number="2.7.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0019197 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase complex [Evidence IEA];
                     GO_function: GO:0015155 - lactose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactose-specific PTS transporter subunit EIIC"
                     /protein_id="HIP6606164.1"
                     /translation="MNKLIEFIEKGKPFFEKISRNPYLRAIRDGFIAAMPVILFSSIF
                     LLVAFVPNIFGFTWSDEAVAAIMKPYGYTMGIVAVLVAGTTAKSLTDAFNRQLPKTNQ
                     INFISTMIASISGFLLLASDGIEGGFANGYMGTKGLLTAFLAAFITVNIYKVCVKNNV
                     TIRMPDEVPPNVSQAFKDVIPYALSIFVLYGIDLVTRQFLGTNVAEAILKLFEPLFTA
                     ADGYVGITIIFGAYALFWFVGIHGPSIVEPAIAAITYANIETNFQLLQAGQHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNKAIGRASVVPTFFGVNEPILFGAPLV
                     LNPVFFVPFILAPIANVWIFKFFVDTLKMNSFSVNLPWTTPGPLGIVMGTNFAPLAFA
                     LAILLVFVDVLIYYPFLKVYDEQILAEEQSGKVENSLKEKVAANFNTAKADAILEKAA
                     VETEISEQTNVLVLCAGGGTSGLLANALTKAAKEYGVPVTATAGSYGAHREILPEYQL
                     VILAPQVASNYDDIKQETDALGIKLAKTEGAQYIKLTRDGQGALDFVKQQF"
     gene            complement(5938..6255)
                     /locus_tag="AC1PIW_001229"
     CDS             complement(5938..6255)
                     /locus_tag="AC1PIW_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HIP6606165.1"
                     /translation="MNREEITLLGFEIVAFAGDARSRLMEALAAAQKGDYAKAEELVE
                     AANACIVEAHHAQTSLLQKEAAGEDLAFSVTLMHGQDHLMTTLLLKDMMSHMIELYKR
                     GDK"
     gene            complement(6287..7120)
                     /locus_tag="AC1PIW_001230"
     CDS             complement(6287..7120)
                     /locus_tag="AC1PIW_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HIP6606166.1"
                     /translation="MFRIIQSLNNNAALVKNEHGEQAVVMGLGITFQKKKGDLIIKDK
                     IENIFSLKNDEAKENFLTLLKDIPLDFISATYTVINHLVETYHYPVQEYLYVTLTDHI
                     YCAYQAVLKNTYQESRLPNISAEYPLEYKMAREALAMFREKLLNDFPNDEIGKIALHL
                     INAKGETIHPTSEEHDISKKIIAEVEQILIDNGIRRSKENSNFYDRFMIHLSYFLNYL
                     DRSHQSNESIASLEQYIKLQNPRAHQVGSQIFEMITSLVDEPVNDSERFYIVLHIQRL
                     L"
     gene            complement(7309..8286)
                     /gene="lacD"
                     /locus_tag="AC1PIW_001231"
     CDS             complement(7309..8286)
                     /gene="lacD"
                     /locus_tag="AC1PIW_001231"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HIP6606167.1"
                     /translation="MVVTADKRKYMEKVSNEAGIISALAFDQRGALKRMMAKHQTEEP
                     TVEQMEELKKLVSEELTQFSSSILLDPEFGLPASRARDEQCGLLLAYEKTGYDTSSTS
                     RLPDCLVEWSVKRLKEEGADAIKFLLYYDVDGDPYVNLQKHAYIERLGSECQAEGLPF
                     FLEILSYDETIEDNASVEFAKVKPRKVNEAMKVFSDERFGVDVLKVEVPVNMNFVEGF
                     GTGEVVYTKEEAAEYFRQQEASTHLPYIYLSAGVSAKLFQDTLVFAHEAGAKFNGVLC
                     GRATWAGVVPVYIEHGEEAAREWLRTVGRENIEGLDVVLSQTATSWLEK"
     gene            complement(8288..9221)
                     /locus_tag="AC1PIW_001232"
                     /pseudo
     CDS             complement(8288..9221)
                     /locus_tag="AC1PIW_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250260.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
     gene            complement(9231..9746)
                     /gene="lacB"
                     /locus_tag="AC1PIW_001233"
     CDS             complement(9231..9746)
                     /gene="lacB"
                     /locus_tag="AC1PIW_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HIP6606168.1"
                     /translation="MKIAIGCDHIVTYEKIAVVDYLKNQGYEVLDFGTYDNVRTHYPI
                     FGKKVGEAVVSGQADLGICICGTGVGINNAVNKVPGVRSALVRDMTSALYAKEELNAN
                     VIGFGGKITGELLMCDIIEAFIKAEYKPTEENKRLIEKIAAVETLNQEQANPEFFTEF
                     LEKWDRGEYHD"
     gene            complement(9773..10195)
                     /gene="lacA"
                     /locus_tag="AC1PIW_001234"
     CDS             complement(9773..10195)
                     /gene="lacA"
                     /locus_tag="AC1PIW_001234"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HIP6606169.1"
                     /translation="MTIIIGADPAGSKLKDVIKDYLLEKSYDFKDVSDGSDFVDTTLA
                     VVAEVNQQEGNLGIVIDAYGVGPFMTATKVKGMIVAEVSDERSAYMTRGHNNSRIITI
                     GSEIVGEGIAKNIVKGFVEGEYDGGRHQIRVDMLNKMC"
     gene            complement(10400..11167)
                     /locus_tag="AC1PIW_001235"
     CDS             complement(10400..11167)
                     /locus_tag="AC1PIW_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIP6606170.1"
                     /translation="MNKRERLEEITRLVNKKGTVRISEIVELLNVTDMTVRRDFIELE
                     EQGVLTKIHGGARSNKAFQYREYSHSEKHIQNKEAKQEIAERAAQLIEDGDTVFLGPG
                     TTVAFLAEAIKNLRLTVITNCMPVFTILSQKKTEDFQVFLLGGEYRQVTESFVGEITN
                     TSLEKMRFSKIFFSGNGLKGTDVMTSTLAEAYTQNIAIKHSLEKYLLLDATKIGKDDF
                     TSFCDLNDATALVTDMNAKDENYQLLSNHIEIISTKS"
BASE COUNT         3439 a         2314 c         1761 g         3673 t
ORIGIN      
        1 cagttatttt agaaaaaata atattcattt tccttgaata aaataaggac acgatttaca
       61 ataagattat gaatacctta atagaatata tcggtccgat tatcctcata gtactaataa
      121 tctcttatag catctatcgc agatgccatc ctaataatat ggacactaaa caagcgctca
      181 aatgtccaaa atgtaagtca ataaaactgc aaacttacat tccaacacaa aaaagatatt
      241 ctcgtaaagc cggtatgatt ggtcatgcaa tcaatggcat agagggagct atgtttgcaa
      301 tgctctatga agctgatcgg acttattatc gttgtcaaca ttgcggttac cgattttcta
      361 gaaaggagaa tcgttttatt tccaacatcg aataacctcc ctgattttta gagaattttg
      421 ctattgaaaa cgaagaattc tcacccctta caaatcccgt tatatcaaca tttacgaaaa
      481 tgaaacttcc atgaaaatgg actaaaatcc tctgatttgg ataaaatcga caccaattaa
      541 caccataaaa tagactagtc atcagtaggt caaactaatt tgacctactt tatcttatag
      601 atggataatt tattaatcaa attcaaaatg ataggtcaag tattttataa atttatcgat
      661 ataagaagac aaaaaaaact aatcaatacg agttattctt caagaaaaaa tcttttcaaa
      721 attatttctc ttttaggaag ttcgacttgt tgctgattat ctctatataa aagcattata
      781 taactcgata caagccgtat atatggactt aatttctata gcctcaaaat aatagtatag
      841 aataccaatg tctaatcttt ctgttgaaaa gaccatctct ttatctaagt gagccaagag
      901 tttgtcgcga agcaatttga tttcatcaat atttttttgt tctttatcaa taccctcttg
      961 aatggtactc aaaattgata aaagagatct atctttggta ctatgtctca acttagatag
     1021 aatcttaaaa ataccatttt tttctctgac atcaaaaatt ttagttatac cgaagacaac
     1081 tctataaaat aaagattcat atgttacatt tcgtactata tcatatctaa tagatgattg
     1141 cactactcta tcatgccact catcgtttac aatttttact atttcaagtg cagcaattat
     1201 tgatccaagt tcaagtatca tagtatgaaa atgaatcatt attttagagt catcatctgg
     1261 taattcatca gcgaaatttt ttcctacaaa atcttttaaa tcacaatcat tcataagcta
     1321 acacctattt aataaatctt cttactttta ctcaaactga taattcattc tgcttcaaaa
     1381 atattttcga gattacaagc atatgtaatg tcctctctca ctaagtatat cataccccat
     1441 aggaatacat ctactacgat ttttgtatcg ctttatcatc tcgtaattaa gtcattgata
     1501 aagttttctc aaaatacagc tgaacaccct gcaacactta atttaatttt tcataatatt
     1561 aaattaagca ataaacccca ctgacttatt cagccagtgg ggtttttgag tgttttccgt
     1621 agaatattta aattacttaa ctatggtatt tactcaataa taatatcaaa atattttgta
     1681 tcaacctctt ttggagcgac gtaactaata tttcgtttgg cattgaatat gtcagattct
     1741 tccagtgatg tagagagtcc gctccatggt tctaatgcga taaagggact ctgatttgta
     1801 gttgaccaaa tcactaaata tggaaaatca tcaaatgcaa tcgaaagttt cttctgatga
     1861 ttttttgatt tcaaggttac ctttcgtgat gctaattgat ccaatgttat ggcatcatac
     1921 tcaaacagtt tataggacaa gtccaaggtc ttctgatttt ccaagaatgg ccggcgatct
     1981 cgcagatcaa gtaagccagt ttctggaaaa cttctaggta ctgtacaaga ttctactttt
     2041 tcaaattcta aataatagtc ttcataactc tcaccatcca acaagggaca attaaaacct
     2101 gggtgacccc caataaagta aggtagcttt ctatgtccct caaggttttt cacccgatat
     2161 tcaattcgaa ttgttttgcc aaatagtgag tagttaatag aaagttcaaa ttcaaaaggg
     2221 taatttttta cggtttcttc gtttgatgaa atagaaaatt ctaaagagtt ttcagtactt
     2281 tttacatttt tgaacagcat ttttctcaca agtccatgcc ttggaattgt gcctgtaaat
     2341 gtcggtcttt ctgcaggttc atagatagcc caatcatttc gtaaacttcc acaaatcgga
     2401 aatagaactg gtgcttgacc gctccaatag gtcggttctc cttgccaaag atattcgata
     2461 ccatccttgt ctttaataga aataatttgc ccacctaatt cagaaaattg tactgttaag
     2521 ttctcatttt ttaattcgag catataaact gcctccctta tatgaaaaca aggatgctaa
     2581 cttgagatag gcatccttgt attagtttta ttcaataacc tgtgtttccg ctactttttt
     2641 ataccaataa gcacttttct ttggataacg ttcttgggtc tcaaagtcaa catagaacaa
     2701 tccatatcgt ttctcgtatc catttgacca agaaaataca tccatcaagg accagatgaa
     2761 ataaccttta acattggcac catctgagat agcatctgaa atcacttcta aatgcttttt
     2821 cacataatca attcgaccat cgtcgtagac tgtaccatct acaaattcat ctttatagcc
     2881 tagaccattt tcagtgatgt agattttctt atagtttgga tagtcatttt ttacacgcat
     2941 gatttgatca taaagacctt gtgggtagat aatccaatcc caatccgttt tcggaatatc
     3001 gactggagat tctctacgac caactccctt gatttgatac ttagaacttc ccttttcacc
     3061 tttaccatta tggataattt ccgtttctcc atcatgagct ctcatccaat cactcatata
     3121 gtaattgatg cccaagaagt cgttcaaatc ttttgcggca tctaaggcag cgaagtcttc
     3181 atcacgaagg tctaattccc caccatttac tttcagaata tgtcgaacgc cttcaagcgt
     3241 tttttcagaa tagtaaccca agtatgtagc atcgaggata aatttattat gaataatatc
     3301 ttctagttcc gctgcacgaa catcgtcagg attggttgga tcataaggat atttcgttgg
     3361 caatgcgtgt acaacaccaa tttctccact ataaccagca tccttgtaaa gcttaacagc
     3421 cttggcatgt gaaaccatca tattgtggtg agattggaat accttagcta agtcatactg
     3481 aatccctggt gggaacttcc caactagata ttgcccatca ccaattggac cgatttcatt
     3541 aaaggttgtc cagtaactaa cttcagggaa ttctttgaag caaaacgctg cgtaattgac
     3601 gaagtgctca atattttctc gatttagaaa gtcaccatta gaatgtagcg tttctggtgt
     3661 atcaaaatgg tgaagcgtta caaatggttc tacgtgacgc ttatggcatt ccgcaaataa
     3721 tttatggtaa tactctactc cctttggatt cacttctcca aagcctgttg ggaagatacg
     3781 agaccacgca attgaaatcc gaatgccatt taccccaaac tgttcactta gttctaaatc
     3841 aactggataa cgattataaa aatcagaagc tggttccgca gtgtaccagt aattatcttc
     3901 tagatacttg tcccacgcaa cacgaccttt tccatctgtc tttgtggcac cctctgcttg
     3961 atatgcagca gttgctccac caaatataaa atctttcggt aatgttttaa ccatatgcct
     4021 accttcttct atcaaaaata aataaagggg taggagttgg agatgataac tccctcccct
     4081 tttgattaag aacctgtatt cacaagtgaa gtgcttgtat atacttgtat ataagagata
     4141 gtttttcgct aatcaaggcg tttttttgag ggaatactga ctgtattttg aaaaaaacta
     4201 acgatgagta gctgaaaaac tagccttaga tggaagtgct gaactgtgaa tataggttct
     4261 aaaactgttg tttgacaaag tcaagagcac cttgaccatc tcgtgtgagt ttaatgtatt
     4321 gagccccttc agttttggca agtttaatac ccaatgcatc ggtttcttgt ttaatgtcat
     4381 cgtagtttga tgctacttga ggtgcaagga tgactaattg atactctggc aaaatctcac
     4441 ggtgcgcccc atagcttcct gctgtagctg taactggaac accatattct tttgcagctt
     4501 tagttagagc gttggcaagt aatccacttg taccaccccc tgcacaaagt accaaaacat
     4561 tagtttgttc agaaatttcg gtttcgactg cagccttttc aagaatagca tccgccttgg
     4621 cagtattaaa attagctgcc actttctctt tcaagctatt ttcaactttc cctgattgtt
     4681 cttcagcaag aatttgctca tcataaactt tcaagaatgg ataatagata agtacatcga
     4741 caaataccaa caaaatagct agtgcaaagg caagtggagc aaagtttgtt cccattacaa
     4801 tccccaatgg tcctggagtt gtccatggca agttaacgct gaaactgttc atttttagcg
     4861 tatcaacaaa gaatttgaaa atccatacgt tagcaattgg agctaagatg aatggaacaa
     4921 agaatactgg gttgagtaca agtggcgcac cgaacaagat cggttcgttt acaccaaaga
     4981 aagttggaac aactgaagca cgtccaattg ctttatttcg tttagactta gtcaaccaca
     5041 taaacatgaa tggcacaacc aaggtagcac ctgtaccacc catagtcacg ataaacattt
     5101 gagtacctga agtcaggatt ttgtccgcat gttgaccggc ttgtaaaagc tggaagttgg
     5161 tttcaatatt agcataagta atggctgcaa ttgctggttc cacaatagat ggaccatgaa
     5221 tccctacaaa ccagaacaaa gcataggcac caaagataat tgtaatacca acataaccat
     5281 ctgcagctgt aaacagaggc tcaaataatt tcagaatcgc ctcagcaacg tttgttccaa
     5341 ggaattgacg tgtcacaaga tcaattccat acaagacaaa gatagacaat gcatatggaa
     5401 tcacatcttt aaatgcctgg gatacattcg gtggaacttc gtctggcata cgaatagtga
     5461 cattgttttt cacacagacc ttatagatat taaccgtaat gaaggctgct agaaaggctg
     5521 tcaaaagtcc cttagttccc atgtaaccat tggcaaatcc accttcaata ccatcagaag
     5581 ccagtaataa gaaacctgaa attgaggcaa tcatggttga aatgaagtta atctgattgg
     5641 tttttggcaa ttgacggtta aacgcatctg ttaaagattt tgcggtcgtt cctgcaacta
     5701 agacagctac aatacccatt gtataaccgt aaggtttcat gatagctgca actgcttcat
     5761 ctgaccaagt aaaaccaaag atattaggaa caaaagccac caatagaaag atacttgaga
     5821 acaagataac cggcatggca gcaataaaac catcacggat agcccttaaa taagggtttc
     5881 tcgaaatctt ttcaaagaaa ggcttccctt tctcaatgaa ttcaatcaat ttattcatta
     5941 cttatcacct cgtttgtaaa gttcaatcat atgactcatc atatctttca ataacaacgt
     6001 tgtcatgaga tggtcttgac catgcatgag cgtcacacta aaggctaaat cctcaccagc
     6061 cgcttctttc tgcaacaaac ttgtctgagc atggtgggct tcaacaatac aagcattggc
     6121 cgcttcaact agttcttctg cttttgcata atcacctttt tgagcagcag ccagtgcctc
     6181 catcaaacga gaacgagcat caccagcgaa tgcaacaatc tcaaaaccta ataacgtaat
     6241 ttcttctctg ttcataatat gaacctcctt ttaaaattga attgatttac aataaccgtt
     6301 gaatatgtaa cacaatatag aacctttcac tatcatttac tggctcatca accaatgatg
     6361 taatcatctc aaaaatctga cttccaacct gatgggctct tggattctgg agcttgatgt
     6421 actgttccaa actagcaatc gattcatttg actgatggga tctatctaag taattcagaa
     6481 aataggacaa atgaatcata aagcgatcat aaaaattgct attttctttt gagcgtctaa
     6541 tcccattatc tatcaaaatt tgttcaacct cagcaataat tttcttactg atatcatgtt
     6601 cttcacttgt gggatgaatt gtttcacctt ttgcattgat taagtgaagt gcaattttac
     6661 caatctcatc atttggaaaa tcatttagca acttttcacg aaacatagct aaggcttcac
     6721 gcgccatttt atattccaat ggatattcgg ctgaaatatt gggtagccta ctttcttgat
     6781 aggtattctt taaaaccgcc tgatatgcac aatagatatg atctgtcagt gtcacatata
     6841 gatattcttg tacagggtaa tgataagttt ctactagatg attaataacc gtgtaggttg
     6901 ccgaaataaa atctaatgga atatctttta ataaggttag aaaattttct ttggcttcat
     6961 cattttttag cgaaaagata ttttctatct tgtccttaat aattaaatcc cctttcttct
     7021 tttgaaaggt tatacctaat cccatcacga ctgcctgttc cccatgttca tttttcacca
     7081 gggcagcgtt attatttagt gactgtataa tcctaaacat atatccctcc tatattttta
     7141 agcaaaaaaa gactacaaac aaaccgagaa tatcctactc tcaaatccat ttgtagtctt
     7201 gcctaatcga attagttaca atccactcta ttcttgctga tgagattatt atagcatatt
     7261 ctcagaaaat gtaaaccctt tcttttaaga aaatggttat tttttcaatt atttttctag
     7321 ccaagaagtt gctgtttgag acaagactac atcaagtccc tcgatatttt cacgaccaac
     7381 agttcgtaac cattcacgcg cagcttcttc accatgttca atatagactg gcacaacacc
     7441 cgcccatgtt gcacgaccac aaagaacacc gttaaattta gcacctgctt catgtgcaaa
     7501 cactagtgta tcttggaaga gttttgcaga aacacctgca cttagataaa tataaggcaa
     7561 atgagttgat gcttcttgtt gacggaaata ttcggctgct tcttcttttg tataaacgac
     7621 ttcccctgtt ccaaagcctt ctacaaaatt catgttcaca ggtacttcta ccttgagaac
     7681 atccactcca aatcgttcgt ccgaaaatac cttcatagcc tcatttactt ttcgaggttt
     7741 gacttttgca aattccacac tggcattgtc ttcaatcgtt tcatcgtagc tcaaaatttc
     7801 caaaaagaat ggcaaacctt ctgcctgaca ttctgaacct aagcgttcaa tataggcatg
     7861 tttttgaaga ttgacgtatg gatccccatc aacgtcatag tagagtagaa acttaatcgc
     7921 atctgcccct tcttctttta gtcgttttac agaccactct actaggcaat ctggtaatcg
     7981 actagtagag ctggtatcat agccagtttt ttcataagct aaaagtagac cacattgttc
     8041 atcgcgggcg cgcgatgctg gcaaaccaaa ttcagggtct aacagaattg aggaagaaaa
     8101 ctgtgttaat tcttctgaaa ctagtttctt tagttcttcc atctgctcca cagtaggttc
     8161 ttctgtctga tgttttgcca tcattctttt cagggctcca cgttggtcaa aagctaaagc
     8221 tgaaataata cctgcttcat tgcttacttt ttccatgtat tttcttttat ctgctgttac
     8281 taccatatta tacctcttca acttcgattt gagaatacaa cgattctgca ttttctaaat
     8341 tgacgtaacc tgtctgttct tcctgagcat ttaacatgcc taaaacatta gcatttttta
     8401 ataattctgc ttcaggtaaa gcatgtacca aacctgctgc aatacctgca acagttgaat
     8461 ctcctgatcc tactggattg acaactttga tttttggaat tctgacacga tagaacttgt
     8521 cattgtgctt ggcaaaagcc ccctttgctc ctaatgagac gactatccaa tcaattcctt
     8581 ggaaaagttg atcagacaaa actgatttca actcttgaat atcatcagta acttctttac
     8641 caatcagttg tgataactct tctgtattcg gcttaataac tgtcggcttc tgctggcttt
     8701 ctaaaacgtt tttcagcgcc tcaccagaac aatccaaaac aacagctaca ccgtattttt
     8761 tacaaagttc tacaatctca acataataat tgctagcaag tcccttaggc aaacttcctg
     8821 aaatgacaac tacatctaca ttgttcagga cgatttcaag gtgttctata aaattcaagg
     8881 cttcatgttc ttgaataatt gggccttgtt ctaaaatctc cgtctgtttg ccatcatgaa
     8941 gaatcgcaat gcaattacgt gtttcgctcc caatttccac aaaggaattt ttaatatcat
     9001 ttcgagtgag ttgtttcttt acataacttc caatctcacc accaataaaa ccagtagcca
     9061 ctatatcttc accaatttgt ttcaaaactc gtgtgacatt gagccccttg cctccagctg
     9121 ttttttgaac ttttttctac tcgattgacc gtatccaaat gaaaggtatc caagtgatag
     9181 gcaatatcaa ccgacggatt taaagtgatt gtcaaaatca tgtgagcctc ctagtcgtgg
     9241 tattctccac gatcccactt ttcaagaaat tctgtaaaga actctggatt agcttgctct
     9301 tgattgagcg tttcaactgc agcaatcttt tcaattaagc gtttgttttc ttcagttggt
     9361 ttgtattctg ctttaataaa ggcttcaatg atgtcgcaca tgagcagctc gcccgtaatc
     9421 tttcctccaa aaccaatgac gttggcattt aactcttctt ttgcatagag tgcagaggtc
     9481 atatcacgaa ccagggctga tcggacgcca ggtaccttgt ttacagcatt gttgattcca
     9541 acacctgttc cacaaataca aattcccaaa tctgcttgcc cgctcacaac tgcttcacca
     9601 acttttttac caaagattgg gtaatgagta cgaacattgt cataagtccc aaagtccaat
     9661 acttcatagc cttgattttt caaataatct accacagcga ttttttcata ggttacaatg
     9721 tggtcacaac cgattgcaat tttcatctct tattctcctt tgtttacaac cattaacaca
     9781 ttttatttag catatcgaca cgaatttggt gacggccacc atcatactca ccttcaacaa
     9841 atcctttaac gatattttta gcaattcctt ctcctacaat ctcactaccg attgtaataa
     9901 tacgcgaatt gttgtgtcct cttgtcatat aggcagagcg ttcgtctgaa acttctgcaa
     9961 cgatcatgcc tttgacttta gttgcggtca taaatggacc aacgccataa gcatcaatca
    10021 caatgccaag atttccttcc tgttgattaa cttctgcaac tactgccaaa gttgtatcaa
    10081 caaaatcact tccatcactg acatctttaa agtcatatga tttttctagc aaataatctt
    10141 taataacgtc ttttagtttt gagcccgctg gatcagcacc gataatgatt gtcataactt
    10201 atctcctaac aaaaatagaa agttgtagcc atatttggca gaaaatcttt gttttgcaaa
    10261 acttagaaaa gcgttttcgc tttttatgtt taaattatac aaccaaacaa acacaaagtc
    10321 aacatatttt attttatttt tgtttgtttt tttcgtatac aaacaaaaaa ccaacatttc
    10381 tctgttggtt ttgataactt tatgattttg tagaaataat ttcaatgtga ttacttaata
    10441 attggtaatt ttcatccttt gcattcatat ctgtcactag agcagttgca tcattcaaat
    10501 cacaaaaaga agtaaaatca tcttttccaa ttttcgtggc atccagcagt agatattttt
    10561 ctaaagaatg tttaatggca atattttgcg tataggcttc cgctagggta gaggtcatga
    10621 catcagttcc cttaagtccg ttacctgaaa agaaaatctt gctgaatcgc attttttcta
    10681 aacttgtatt ggtaatttct cctacaaaag attctgtcac ttgccgatat tctcctccaa
    10741 ggagaaagac ttgaaaatcc tctgtcttct tttgtgatag gatagtaaaa acaggcatac
    10801 aatttgtgat gacagttagg cgaagatttt taatggcctc agccagaaag gccactgttg
    10861 ttcctggacc taaaaatacc gtgtctccat cttctatcag ttgtgcagcc ctttccgcta
    10921 tctcttgctt ggcttcctta ttctgaatgt gtttctcaga atgggaatat tctcgatatt
    10981 gaaaagcctt attacttcta gcaccgccat gaatttttgt taagacacct tgctcttcca
    11041 attcaatgaa atcacgtcga acagtcatat cagtaacatt tagcaattct actatctcag
    11101 aaattcgaac tgtgcctttt ttgtttacca atcgtgtaat ttcttctagt cgttctcgtt
    11161 tattcatgtt taatcctttg ttgtttt
//
LOCUS       DBNFCK010000060        10818 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.060, whole genome shotgun sequence.
ACCESSION   DBNFCK010000060 DBNFCK010000000
VERSION     DBNFCK010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10818
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.060"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            155..1987
                     /gene="lepA"
                     /locus_tag="AC1PIW_001236"
     CDS             155..1987
                     /gene="lepA"
                     /locus_tag="AC1PIW_001236"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HIP6606171.1"
                     /translation="MNLEDLKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPIINKIDLPAADPERVRQEIEDVI
                     GLDASEAVPTSAKAGIGIEEILEQIVEKVPAPTGDVEAPLQALIFDSVYDPYRGVILQ
                     VRIVNGVVKPGDTIQMMSNGKTFDVTEVGIFTPKAIGRDYLATGDVGYIAASIKTVAD
                     TRVGDTVTLAENPASEPLAGYKQMNPMVFAGIYPIDSNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERIEREFNIDLIMTAPSVVYHVNMTDGEMID
                     VANPSEFPDPTKIATIEEPYVKAQIMVPQEYVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYEISEYRSSKLVKMDILLNGDKVDALSFI
                     VHKEFAYERGKIIVDKLKKIIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKAIGSVEVPQEAFLSVLSMDEDDKK"
     gene            complement(2037..2219)
                     /locus_tag="AC1PIW_001237"
     CDS             complement(2037..2219)
                     /locus_tag="AC1PIW_001237"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HIP6606172.1"
                     /translation="MPFVRIDLFEGRTEEQKIALAREVTEVVSRNTNAPKEAIHVFIN
                     DMPEGTYYPQGEMKRK"
     gene            2354..3661
                     /locus_tag="AC1PIW_001238"
     CDS             2354..3661
                     /locus_tag="AC1PIW_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HIP6606173.1"
                     /translation="MFKKKATQIVSETSIKKSRKFLNRKTAGITGGVLLTTFIGSQLM
                     TAELPKKDDLYGQQYVTVVKHLQEAGLKNIQGVEITDLEFGKIGESNLVELVSVDGED
                     WKEGRTLKNIPITISYHVPKKDAVEFNLPASKNLADVEKELKDSGFMQIELTPVLLVE
                     EGNADKKDKIDRLQIGNHTYQSNHFYSTSLPVTLTYFDVSKDNIKLPENLAEAKTKLE
                     LEKQLKTAGFTDIKWTAVADKDKAKHEKIQKINLGGAEIQLPTKQEIISKKSTPIVIT
                     YYDFSSFAELPSSISTKTAADTKKLFTDGGFSQVSEVATETNEIAKNGQIIAVEIDGK
                     SFNEMNDKVIKKDSKVIVKYWNAEKAIAEKARKEEEERLAAEAQKVAEAQSQVQQFTA
                     TPSQNTYYPNCKAVRQAGAAPIYHGEPGYGSHLDRDGDGVGCE"
     gene            4046..4627
                     /locus_tag="AC1PIW_001239"
     CDS             4046..4627
                     /locus_tag="AC1PIW_001239"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HIP6606174.1"
                     /translation="MAQLYYKYGTMNSGKTIEILKVAHNYEEQGKPVVIMTSALDTRD
                     AFGVVSSRIGMRRDAVAIDDDMDIFGFIEKMEPRPYCVLIDEAQFLRRHHVYALARVV
                     DELDVPVMAFGLKNDFRNELFEGSQHLLLLADKLDEIKTICQYCSKKATMVLRTQDGK
                     PTYEGEQIQIGGNETYIPVCRKHYFSPEIKDLP"
     gene            5078..6157
                     /gene="prfA"
                     /locus_tag="AC1PIW_001240"
     CDS             5078..6157
                     /gene="prfA"
                     /locus_tag="AC1PIW_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIP6606175.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEATTR
                     DTVTAYREYKTVLQNIVDAEEMIKESSGDADLEEMAKEELKQAKADKEAYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEGQGWRFEVMEASYNGVGG
                     IKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLIMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKMDEVVDALVLYDQTQKLEELNK"
     gene            6157..6990
                     /gene="prmC"
                     /locus_tag="AC1PIW_001241"
     CDS             6157..6990
                     /gene="prmC"
                     /locus_tag="AC1PIW_001241"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002934985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIP6606176.1"
                     /translation="MNYAQLFAAYEEQLVAIGEEAESLSFTFRGLKGLNFTEFLLLLR
                     QEVTPTDKEEIDAIFQQLSQHRPAQYIIGKADFHGLEFAVDERVLIPRPETEELVDLI
                     LQENSGASLRILDIGTGSGAIAISLAKARPDWEVVAVDISNDALAVAQENARTNQVSV
                     HFLESDVLQAVTGKFDIIVSNPPYISPDDTDEVGLNVLTSEPHLALFAEEDGMAIYRQ
                     IAEQAGAFLKENGKLYFEIGYKQGQDLTDLLALHFPQKQIRVLKDQFGQDRKVVADDN
                     G"
     gene            6977..7585
                     /locus_tag="AC1PIW_001242"
     CDS             6977..7585
                     /locus_tag="AC1PIW_001242"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIP6606177.1"
                     /translation="MTMDKLRTILENGGAVILPTETVYGLFAQALNEDAVNRVYQLKQ
                     RPRDKAMNLNVSNLNDIYFFSQNTPFFLEKLYNRFMPGPLTIILKANDNVPFWVNSGL
                     DTVGFRLPNHEQTLRLISETGPLIGPSANISGNESGKKFSDIQAQFSVDLPGIEDDQA
                     LTGIDSTILDLSGQKARILRQGAISREEIQKAIPEIEFEVVL"
     gene            7582..8013
                     /locus_tag="AC1PIW_001243"
     CDS             7582..8013
                     /locus_tag="AC1PIW_001243"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6606178.1"
                     /translation="MIRPIEKSDLIVIREINAQSLGYDCSLEQTERQFFRCTSTLGHI
                     LLVYIDDISGAVQGYIHAQVYESLYSDTGLNILGLAVLPGHQGQGIGASLLKAVEQIA
                     QKEGYHFIRLNSAESRLQAHLFYEKNGYHSDKMQKRFIKHI"
     gene            8037..9296
                     /gene="glyA"
                     /locus_tag="AC1PIW_001244"
     CDS             8037..9296
                     /gene="glyA"
                     /locus_tag="AC1PIW_001244"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIP6606179.1"
                     /translation="MIFDKVNYKEFDKEVWEAIQAEEKRQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTECVDVVESLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMALIEPGDTVMGMDLAAGGHLTHGASVSFSGQTYNFVAYNVDEETGLLDYDAIL
                     KQAKEVQPKLIVAGASAYARTIDFAKFREIADAVGAKLMVDMAHIAGLVAAGLHPNPV
                     PHAHITTTTTHKTLRGPRGGLILTNDEELIKKINSAIFPGIQGGPLEHVIAAKAVSFK
                     EVLDPAFKDYAQKVIENSKAMAEVFLANPNFKVITGGTDNHLFLVDVTKVVENGKVAQ
                     HLLDEVNITLNKNSIPYEKLSPFKTSGIRIGSAAITARGFGVEEARKVAQLTIKALEN
                     AENEKALEEVRQEVRALTDQFPLYEGL"
     gene            9296..10270
                     /locus_tag="AC1PIW_001245"
     CDS             9296..10270
                     /locus_tag="AC1PIW_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HIP6606180.1"
                     /translation="MDIFVKKAIIHQFSPDDTELVLSDQLLTVSPKIEEYLRKKIERV
                     FSDEAKTGQFNPDNPFLDYLDGDLLTNSVKIANLWKEEFSISENLKTNDLIFIEFERN
                     GVEHFAFLRISLRENLAHVGLESGSPLKITQNNLPGAGSAPDEALIVNLQTRKYHLIE
                     KRIKHNGAFLNYFSDNLLQVTPAISAKKSIKAVEQTAQKIADNFHQGDFQFQSKVKSA
                     IFNNLEEDNELSPEKLADQLFDNNLTARLNFVDQLKEVIPDKISFDEIDSSRQLKKFE
                     NQKLSLSNGIELIVPNAIYEDAESVEFIQNDNGTYSILIKNIEDIKSK"
     gene            10271..>10818
                     /locus_tag="AC1PIW_001246"
     CDS             10271..>10818
                     /locus_tag="AC1PIW_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HIP6606181.1"
                     /translation="MKKLIRTLILLLLIFLGYKFYQTYNAVKQVMTYQTMVQEVLAEN
                     DTPANEELVLAMIYTETKGQEADVMQSSESATGYANTITDSRESIRQGIIYLTENLRL
                     AEEKGVEVWTAVQAYNFGPAYIDYIAEHGGEHTLSLAKEYSRTVVAPSLGNTTEETYT
                     YFHPLALLNGGKLYVNGGNIYYA"
BASE COUNT         3420 a         2164 c         2285 g         2949 t
ORIGIN      
        1 ataattcctt gtttcagtct tattttatga ccgactagaa cttactttgg gaatttactc
       61 gtaacaataa cctatatttc taggtgtttt tcttatggaa aattatcact aaatctgata
      121 taatgaggag taataccctt gtagaggaag aaacatgaat ttagaagatt tgaaaaaacg
      181 acaggagaaa attcgtaatt tctccattat tgcccacatc gaccatggta aatcaacctt
      241 ggctgaccgt attcttgaga agacggaaac agtttccagc cgtgaaatgc aagcccagtt
      301 gctggatagt atggacttgg aacgcgagcg tggtatcaca attaaactca acgcaatcga
      361 actcaactat acagctaaag atggagagac atacattttc cacttgattg acacaccagg
      421 acacgtggat tttacctatg aggtatcgcg ttcgttggct gcctgtgaag gagcgatttt
      481 agtggtggat gctgctcaag ggattgaagc acagaccttg gccaacgttt atctagcctt
      541 ggacaatgat ttggaaatct tgccaatcat taacaagatt gacctgccag cagcagaccc
      601 agaacgtgtt cgtcaggaaa tcgaagatgt gattggtttg gatgcttctg aagcagtgcc
      661 aacatcagcc aaggcaggta ttggtatcga ggaaatcttg gagcagattg tcgagaaggt
      721 tccagcaccg actggtgatg tcgaagctcc attgcaagcc ttgatttttg actcggttta
      781 cgatccgtat cgaggcgtta tcctccaagt tcgtatcgtc aatggtgtgg taaaacctgg
      841 tgacaccatt cagatgatga gcaatggtaa gactttcgat gtgacggaag tggggatttt
      901 cacacccaaa gcaatcggtc gtgattattt ggcgactggt gatgttggtt acattgcagc
      961 ctctatcaag accgttgcag atacccgtgt cggtgatacg gtaaccttgg cagaaaatcc
     1021 tgccagcgag ccactagcgg gctacaagca gatgaatcca atggtctttg caggtatcta
     1081 tccaatcgat tccaacaagt acaatgacct ccgtgaagcc ttggaaaaac tccaactcaa
     1141 cgatgccagc ttgcagtttg agccagaaac atcgcaggca ctcggatttg gtttccgttg
     1201 tggattcttg ggcctcttgc acatggatgt tatccaagaa cggattgagc gtgagtttaa
     1261 cattgacctc atcatgacag caccgtcggt ggtttaccat gtcaacatga cggacggtga
     1321 aatgattgat gtagccaacc catccgaatt cccagaccca acaaagattg ccactatcga
     1381 agagccttac gttaaggcac aaatcatggt accacaggaa tacgtcggtg cagtcatgga
     1441 attggcccaa cgcaaacgcg gtgactttgt gactatggat tacatcgatg ataatcgtgt
     1501 caatgtcatc taccaaattc cacttgcgga gattgttttt gacttctttg ataagcttaa
     1561 gtcgtccact cgtggttatg ccagcttcga ctatgagatt tctgaatacc gttcatccaa
     1621 gttggtgaaa atggatattc tcctcaatgg tgacaaggtc gatgccctca gctttatcgt
     1681 ccacaaggaa tttgcctacg aacgtgggaa aattatcgtg gacaaactca agaaaatcat
     1741 tccccgtcaa cagtttgaag taccaataca agcagctatc ggtcagaaaa ttgttgcccg
     1801 tagcgacatc aaggctcttc ggaagaacgt tttggccaag tgttatggtg gtgacgtttc
     1861 ccgtaaacgc aaactcctag aaaagcaaaa agctggtaaa aaacggatga aagccatcgg
     1921 ctctgtcgaa gttccacaag aagccttcct cagtgttctc agcatggatg aagacgataa
     1981 gaaataaact tgatacaaag aaacactcct gcattttctt gcaagagtgt tttctcttat
     2041 ttacgtttca tttcaccttg tgggtagtag gtcccttcag gcatatcatt gatgaagaca
     2101 tgaatggctt ctttgggcgc attagtattg cgtgatacca cctcagttac ttcacgcgca
     2161 agggcaattt tttgttcttc tgtgcgtcct tcaaacaaat caatacgtac aaatggcata
     2221 tatttcctcc tttatttttc gtttattgta ccatagtttt tatgattctc caaggatatt
     2281 ttcataagat ataacaaaat atatttatat tttgttataa taatatcgcg acaaacaata
     2341 aggagaatga tttatgttta agaaaaaagc aactcagata gttagcgaaa cgtcaattaa
     2401 aaaatctaga aaatttctta atcggaaaac agcaggaata actggtggcg tcctacttac
     2461 aacatttatc ggttcacaat taatgactgc tgaacttcca aaaaaagatg acctttatgg
     2521 acaacagtat gtaacagtcg taaaacatct tcaggaagca ggtttaaaaa atattcaggg
     2581 ggtggaaatc actgatttag aatttggaaa aataggtgaa agcaatcttg tcgaactcgt
     2641 ctctgttgac ggagaagatt ggaaggaagg acgtacttta aaaaacatcc caattacaat
     2701 ctcctaccat gttcccaaaa aagatgccgt tgaattcaac ctaccagctt ctaaaaattt
     2761 agcagatgtc gagaaagagc tgaaagattc aggttttatg caaatcgaac taactcctgt
     2821 tctgctagta gaagaaggca atgcggacaa aaaagataaa attgatcgcc ttcaaatcgg
     2881 aaatcacacc tatcaatcca atcattttta ctctactagt ctccctgtta cattaaccta
     2941 ttttgacgtt agcaaagata acatcaaact accagaaaat ctagcagagg ctaaaacaaa
     3001 actagagctc gaaaaacaac taaaaacagc aggatttaca gacatcaagt ggactgctgt
     3061 tgcagacaag gataaagcaa agcacgaaaa aattcaaaaa attaatctag gtggtgcaga
     3121 gattcaattg cccaccaaac aggagattat cagcaaaaaa tcaaccccca ttgtaattac
     3181 ctactacgat ttctctagct tcgctgaact gccatcatcc atttcaacga aaacagctgc
     3241 agatactaaa aaattgttta ccgacggtgg tttttctcaa gtatctgaag tagcaacaga
     3301 gaccaatgaa attgctaaaa acggacaaat cattgcggtc gagattgatg gaaaatcttt
     3361 taacgagatg aacgataagg tcatcaaaaa agatagcaaa gtcattgtca aatattggaa
     3421 tgctgaaaaa gctattgctg agaaagcacg taaagaggag gaagaacgtc tcgccgccga
     3481 agcgcaaaaa gtagcagaag cacaaagcca agttcagcaa tttactgcta caccatctca
     3541 aaacacatat tatccaaatt gtaaggcagt tagacaagca ggagctgcac caatctatca
     3601 tggagaacca ggttacggta gtcatttaga ccgtgatgga gatggagttg ggtgtgagta
     3661 agaaaaaaca ttgacaacgt ttacattgcg tgctatacta ctcttataag gagggattgc
     3721 tcatgaccta taaataccgt atgattttat cgttcctact cactggatta tttttgtatc
     3781 tagtagtaac tgtttttaac aaaagtgtat gggaaggccc attgtttctt gcattttctt
     3841 tttacagcct gatatatggt tgtgttatgc tctacaagtg gaaaccaacg gcagctaaaa
     3901 tcattttccg atgtattgga gaatttctct ctttaccatg gtcttaatat caaacaagct
     3961 caaaacgggc ttgtttttct atttcctaaa tggtcaattt atgatagaat ggagggagtg
     4021 aaaatttaag attggaaaga gaaaagtggc tcaattatat tataaatatg ggacaatgaa
     4081 ctcaggaaaa accattgaga ttttgaaggt tgcccacaac tatgaagaac aaggaaaacc
     4141 tgttgttatc atgacatctg cacttgatac tcgtgatgcc tttggtgttg tatccagtcg
     4201 tataggcatg agaagagatg cagtggcgat tgacgatgat atggatatct ttggttttat
     4261 tgaaaaaatg gaaccgcgtc cttactgtgt tctcattgat gaagcacaat ttcttcgacg
     4321 tcatcacgtc tacgcacttg ctcgcgtagt cgatgaatta gatgtacccg ttatggcttt
     4381 cggtcttaaa aatgatttcc gaaatgaact atttgaaggt tcccaacatt tgctcttatt
     4441 ggctgataaa ttagatgaaa tcaaaacaat ctgccaatat tgttctaaaa aagcgacaat
     4501 ggttttgaga acacaggatg gaaaacctac ttatgaagga gaacaaatcc aaattggtgg
     4561 caatgaaacc tacattcctg tctgtcgcaa acattatttt tcaccagaaa ttaaagattt
     4621 accctaattt ttgaaaatga aatgagaagc aactgtaaac tgagcaacta tatagaactg
     4681 aatttgccta tgactctgtg ccaattttca taacttacat actacggcaa aggaattgaa
     4741 cacgccctac aagctgtgtc aaaaagataa tttctccaag tgtttgtaac acttggtcaa
     4801 aattcctatt ttgactgtgc ttgcttaacg ggctttgtat cttaatggaa ttgaacacgc
     4861 cctacaagct aactagaagt gtgtgcggtc aaatatttag aaaggaattg aatacgggct
     4921 acgaactgtg cctgtttcat gacctacata ttacagaaag gaattgaaca cgccctacaa
     4981 gctgtgtcaa aaagataatt tctccgagtg tttgtaacac ttggtcgaaa ttcctatttt
     5041 gactgtgctt gcttaacggg ctttgtatct taatcttatg aacatctacg atcaattaca
     5101 ggcggtggaa gaccgctacg aggaacttgg tgaattgttg tctgaccctg atgtagtcag
     5161 cgataccaag cgttttatgg agttgtctaa ggaagaagct acaactcgtg atacagtaac
     5221 agcctaccgc gaatataaga ctgtacttca aaacattgtc gatgcagaag agatgattaa
     5281 agaatcttct ggtgatgctg acttggagga aatggctaag gaagaactca aacaagctaa
     5341 ggctgataaa gaagcttacg aagaaaagct gaaaatcctt cttttaccaa aagaccctaa
     5401 cgatgacaaa aacatcatct tggaaatccg tggtgccgca ggtggtgatg aagctgccct
     5461 ctttgctgga gaccttttga ccatgtatca aaaatatgca gaaggtcaag gctggcgttt
     5521 tgaggttatg gaggcttcat ataacggtgt cggcggtatc aaggaagtgg ttgccatggt
     5581 ctctggtcaa tccgtttatt caaaactcaa gtatgaatca ggagctcacc gtgtccaacg
     5641 tgtccctgtg acagaaagcc aaggtcgtgt ccacacctcg acagcaactg tcctcatcat
     5701 gccagaagtt gaagaagtgg aatacgacat cgatccaaaa gatcttcgtg tcgatattta
     5761 ccacgcatct ggtgctggtg gacagaacgt caataaggtt gcgactgccg ttcgtatcgt
     5821 tcacttgcca accaacatca aggtcgaaat gcaggaagaa cggactcagc agaaaaaccg
     5881 tgaaaaggcc atgaagatta tccgtgcccg tgttgcggat cactttgcac agattgccca
     5941 agatgagcag gatgctgagc gtaaatcaac tatcggtaca ggtgaccgct cagagcgcat
     6001 ccgtacatac aacttcccac aaaaccgtgt gacagaccac cgtatcggct taaccttgca
     6061 aaagctggat actatcttgt caggtaagat ggacgaagtc gtagatgccc ttgttctcta
     6121 cgatcaaacg caaaaactag aagagcttaa caaataatga attacgctca attatttgca
     6181 gcatacgaag aacaattagt cgcaatcggt gaagaggcag aatccctgtc cttcaccttt
     6241 cgtggcttaa agggattgaa ctttacggaa tttctcctcc tgctccgtca ggaagtcaca
     6301 ccgactgaca aagaagagat tgatgcgatt tttcagcaac tttcccaaca ccgacctgcc
     6361 cagtacatta tcggcaaagc tgattttcat ggcttggagt ttgcggtgga tgagcgagtc
     6421 ttgattccaa gacctgaaac ggaagaatta gttgatttga ttttgcagga aaatagcgga
     6481 gcaagtttgc gtatcttaga catcggtaca ggaagcggtg ccatagctat ttcccttgca
     6541 aaagctagac ctgattggga agtggtagcg gttgacattt caaatgatgc cctagcagtg
     6601 gctcaggaaa atgcaagaac caaccaagtc tctgttcact ttctagaatc ggatgtcttg
     6661 caagctgtga cagggaaatt tgacatcatt gtttctaatc cgccctatat ctcaccagat
     6721 gacacagatg aagttggact caatgtattg acttctgagc ctcatcttgc cttatttgcg
     6781 gaagaagatg gcatggctat ctatagacaa attgcggagc aagcaggggc atttttgaag
     6841 gaaaatggaa aactctactt tgaaattggc tacaagcaag ggcaagacct aaccgactta
     6901 ctcgctctgc atttccccca aaagcaaatt cgagtcctca aagaccaatt tggacaggat
     6961 agaaaggtcg tagcggatga caatggataa actccgaaca attcttgaaa atggtggtgc
     7021 tgttatcctt ccaactgaaa cagtctatgg gctttttgcc caagccttga atgaagatgc
     7081 agtcaatcgt gtctatcagt tgaaacagcg tccgcgggac aaggcaatga acttaaatgt
     7141 ctcaaatctg aacgatattt attttttcag tcaaaataca cccttttttc tggagaaact
     7201 gtataataga tttatgccag gaccgctaac gattatcctg aaagcaaacg acaacgttcc
     7261 tttttgggtt aattctggac tggatactgt tggttttcgt cttcctaatc acgaacaaac
     7321 cttacgtttg atttcagaga ctggcccact tattggaccg tccgccaata tttcaggaaa
     7381 cgaaagtggc aagaagtttt cggacatcca ggcacaattt tcagttgatt tgcctggcat
     7441 tgaggatgac caagctctaa caggcattga ttcaacgatt ttggaccttt ctggacagaa
     7501 agcgcggatt ttgcgccaag gtgctatcag ccgcgaagag attcagaaag ccattcccga
     7561 aatagaattt gaggtagttt tatgattcgt cccattgaga aaagtgactt gatagtcatc
     7621 cgagaaatca atgctcaatc cttagggtat gattgttcat tggaacaaac tgagcgacag
     7681 ttttttagat gcacgagcac attggggcat atcttgttgg tctatataga tgacataagt
     7741 ggcgcagtgc aaggttacat tcacgcacaa gtctatgaaa gtctctattc ggatacaggc
     7801 ctcaatattc tcggactagc tgttttgcct ggtcatcaag gacaagggat tggagcaagc
     7861 ctgctgaaag ccgttgagca aattgcacag aaagagggct accattttat tcgcctcaat
     7921 tctgctgaaa gtcgtctcca agcccatctt ttctacgaaa agaatggcta tcactccgat
     7981 aaaatgcaga agcgttttat caagcacatt tagcccaata caaaggagta ccgtatatga
     8041 tttttgacaa agttaactac aaagaatttg ataaagaagt ttgggaagct atccaagctg
     8101 aagaaaaacg ccaacaaaat aatatcgaat tgattgcgtc tgaaaacgtt gtttctaaag
     8161 ctgttatggc agctcaaggt tctattttga ccaataaata cgcagaaggt tacccaggtc
     8221 gtcgttacta tggcggtacg gaatgcgttg acgtagttga aagtttagct attgaacgtg
     8281 ctaaagaaat attcggtgca aaatttgcta acgttcagcc gcactcagga agccaggcca
     8341 attgtgctgc ctacatggcc ttgattgagc caggagacac tgttatgggg atggacttag
     8401 ctgcaggtgg tcacttgact cacggtgcat cagttagctt ctcaggacaa acttacaact
     8461 ttgtagctta caatgttgac gaagaaactg gcttgcttga ttacgatgca attttaaaac
     8521 aagcaaaaga agtacagcca aaattgattg tagcaggtgc ttcagcctat gcccgtacaa
     8581 ttgactttgc taaattccgc gaaattgcag atgcagtggg ggccaaactg atggtggata
     8641 tggctcatat tgctggtctt gtagcagcag gtcttcatcc gaacccagtg ccacatgccc
     8701 atattacaac aactacgact cataaaactc ttcgtgggcc acgtggtggt ttaattttga
     8761 ccaatgatga agagctcatc aagaaaatca actcagccat cttcccaggc atccaaggtg
     8821 gtccattaga gcatgtcatt gctgcaaaag ccgtatcttt caaagaagtt ttggacccag
     8881 ctttcaaaga ctatgctcag aaagtgattg agaacagcaa ggccatggcc gaagtcttcc
     8941 ttgcaaaccc taactttaaa gtcatcacag gaggaacaga caaccatctc ttcctcgttg
     9001 acgtgaccaa agttgttgaa aatggtaaag ttgctcagca tctcttggat gaagtaaata
     9061 ttactctcaa taaaaactca attccttacg aaaaactctc accattcaaa acaagcggta
     9121 tccgtattgg ctctgcggcc attactgctc gtggttttgg agtagaggaa gctcgcaagg
     9181 ttgcacaact aactatcaaa gcacttgaaa atgcagaaaa tgaaaaagct cttgaggaag
     9241 tccgtcaaga agtgcgtgct ttgacagatc aattcccact ttacgaaggt ttataatgga
     9301 tatatttgta aaaaaagcca tcatacatca atttagtcct gatgatacag agctggtcct
     9361 ttccgaccag cttttgactg ttagcccaaa aatagaagaa tatttgcgta aaaagattga
     9421 acgtgtcttt tctgatgaag caaaaacagg acaatttaac cccgacaatc cttttcttga
     9481 ctatttagat ggcgacctac tgaccaactc tgtcaagatt gctaatctat ggaaagaaga
     9541 attttcaatt tctgagaatt taaaaaccaa tgacctcatt ttcatagagt ttgaacgaaa
     9601 tggtgttgaa cattttgcct ttttgagaat ctcactgcgt gaaaatctcg cccacgttgg
     9661 tctggaaagc ggaagtcctt taaaaatcac ccagaataat ttacctggag caggttcagc
     9721 acctgatgaa gccttgattg tcaatcttca aacgcggaag tatcatttga ttgagaaaag
     9781 aatcaaacac aatggagcct tcctcaatta tttctcagat aatcttctgc aagttacacc
     9841 agctatttct gcaaaaaaat caatcaaagc agtagagcaa actgcacaga agattgctga
     9901 caatttccat caaggcgatt ttcaatttca atcgaaagtc aagtcagcta ttttcaacaa
     9961 tttggaagaa gataatgagc tttcacctga aaaattggct gatcagttat tcgataacaa
    10021 cctgaccgct cgtttaaatt tcgtcgatca actgaaagag gtcatacctg ataagatttc
    10081 cttcgatgaa attgatagta gccgtcaact taagaaattt gaaaatcaga agctatcttt
    10141 atcaaacgga attgagttga ttgtgccaaa tgcaatctat gaggatgcag aatcggttga
    10201 gtttattcaa aatgacaacg ggacctattc tattcttatc aaaaatattg aagacataaa
    10261 gagcaagtaa atgaaaaaac tgatacgtac gctgatactt cttttgctga tttttctcgg
    10321 ttacaaattc tatcaaacct acaatgcagt caagcaagtg atgacctatc aaactatggt
    10381 acaagaagta ttggctgaaa acgacacacc agctaatgaa gaactagtcc tagcaatgat
    10441 ttacacagaa accaagggac aagaagcaga tgtcatgcaa tcgagtgagt ccgctactgg
    10501 ctatgctaac actatcacgg atagtagaga gagtatcagg caaggaatta tctacctgac
    10561 agaaaaccta cggttggctg aggaaaaagg agtagaggtt tggacagcag ttcaggcata
    10621 taatttcggt ccagcctata ttgactatat tgctgaacat ggtggagaac atacactctc
    10681 tttagctaaa gaatactcca gaacagtagt agcaccaagc ttagggaata ctacagaaga
    10741 aacatatacc tattttcatc ccttggcttt gctaaatggt gggaagttat atgtaaatgg
    10801 tggaaatatc tactatgc
//
LOCUS       DBNFCK010000061        10593 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.061, whole genome shotgun sequence.
ACCESSION   DBNFCK010000061 DBNFCK010000000
VERSION     DBNFCK010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10593
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.061"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(374..1228)
                     /locus_tag="AC1PIW_001247"
     CDS             complement(374..1228)
                     /locus_tag="AC1PIW_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HIP6606182.1"
                     /translation="MKTVRQIICCLVAIICVLSLPSLSLQAQDSFDVQVHIPFPNGID
                     ASQVSTGLEAWYIASEEPVSSEQLADSLYQKSRSELTSLYGEPISSQALSPEGQATFR
                     GLTKGWYYVRQVGKPTSLEVIPFLMNVGRGVTKIEAKVRRPSEEKGSKPFLKVSTSTA
                     PLSGAEFAVLEEVGGELKEVIVDGNSYHVTSSSNGQFVVGPLPYGTYYLKEVKAPTGY
                     ILSQDTIPFEITSDSHVSEIIKIKNKPVTPPGIEIPYTGNVVVIAVLSTGILLFLLGY
                     RLVTYTKR"
     gene            complement(1252..2361)
                     /locus_tag="AC1PIW_001248"
     CDS             complement(1252..2361)
                     /locus_tag="AC1PIW_001248"
                     /inference="COORDINATES: protein motif:HMM:NF027884.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HIP6606183.1"
                     /translation="MVKLNNNGLAKLEEKAREGEVNFTLTYKATLNDSAKVETEIPNQ
                     VKLIYGNRPSEFSEPKSTTPKDGEIIVNKTWDTGVNQTEVVFGVYEKGTGVRVGEIKL
                     AAGVNTGKLTGLDGAKEYIVIEETIVSGSLPSYSNGDTGTIRIENKKNPNPTPLTPED
                     PKVVTYGKRFVKTDDKDLDSSEKLLGAEFIVRKDGEESYLALKDTVTQAKEIADYKAA
                     EADYLATVKSATTDNPKIDEIKQKKDARDTAYEKMNMQWTWVGTKEGAFTFVSSTDGK
                     FEVKGLKEGKYELIETKAPEGFALPTSPVEFTVGAHTWGTIDALTADNFQQIKNKKVT
                     IPQTGGIGTLVFTIVGLSTMVFAFIAMKKRQSEEA"
     gene            complement(2345..3205)
                     /locus_tag="AC1PIW_001249"
     CDS             complement(2345..3205)
                     /locus_tag="AC1PIW_001249"
                     /inference="COORDINATES: protein motif:HMM:NF027869.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HIP6606184.1"
                     /translation="MKKLTKLFSVFTVFLTVLGSLINIKHLVHAEDTASTTVIVHKIV
                     MNNDDFNKFTYEENLSKYNGNSIGDLKNYFGNSAVEVAGVKFDVWKKTDVVAPEAKTG
                     AELGITGDTNKYVKTDKTAMTQDNGATFTLANGIYIFTEDKENSPYYNNQNELTGMKA
                     VPFKLELPQAKTDGSGYFDTKTPLHVYPKNTENKPEITKEFTDKRTNVDQDGIKNVEI
                     GQEIGYTITTKVPKDAAYKTFAWEDTMLAGLDFKLNSLQIADNKNLNLCSRYRLHIDT
                     NCTWIHGEIE"
     gene            complement(3249..7343)
                     /locus_tag="AC1PIW_001250"
     CDS             complement(3249..7343)
                     /locus_tag="AC1PIW_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HIP6606185.1"
                     /translation="MFPNGNVANSIPQSIELYVADGTKAMSYSKDSLEKLTYKGTSNN
                     GNIHHYYLPGTDIGISVRVYSERNSYPAFDGFESTHTIEFYQEGWKLGNKAIVVRLKE
                     RKKFQKNGLYYNGMSVPWNSPVMGGGRTTNIYRREGDTVSQGGAYPVDWYGFKLKKVG
                     IKNGQKIPLSGAIFTLYKGNEPYRTAVSDDNGDIQFSEIIGGTYTFKETSAPSGYILD
                     PTEHTIVVTDNQNITIDGKKYDVNKPTEVVNTKSTTLQINKFEFGESKPLKGAVFRLQ
                     SKNGGNYDVTIGDVLPASQFIFSNLAKGIYTLTETKAPAGYKTIPPLDIEVYEENGEL
                     KVRKLTDTSNTQTGAVEVVNGNFSINVVDEDFSTEFTKVNEQGQPLADAVFELRQITQ
                     TGYKRVLTGLTSNSQGKLRVDHLQGGITYELWETSAPAGYSKLTTAAARFTVSETGTI
                     SFESGTSERIINRPPTYKVKVQKIDALTGQKVTVQTRIGITDSQGNVIDGQIANFDNG
                     EFSFPKNFTPGTYYIKEEKTPSGYIGLSGLVPFTIRADGIVEVNSDYLEGIDVTTSSP
                     DTITIKVKNYPLGKFKVSKRVKGISDLTNLITGQMTFTLTKNDDVNFAPIVKQQAANQ
                     DFVFENLQPGVYTLTETQAPSGYVKSTENYTIYVNRDGSVRFYSDSDISSHIARGLHV
                     IKSAEIAMASTYGSSTEDKALDGDLTTGALYQLPSSTLNEGQWWGVNLGSPQRVTQIR
                     FAQGKNTTNGSDHDKMDSYALEYSPDGITYQSIGNFTETDLTQTVDIYAQYIRVRNLQ
                     TGTNRWLGIRELQVSVRDATQTIEAGGSDAAANVIKIGNIENPEFKIEKVDANNKQIS
                     KPVTFKLYKVDDSTTEATVSSTSLDDTNLVQTLTFTGQSSLTRLTATALGKYVLVETQ
                     APEGYDGLSAPVLLELYETTQAYAVTQQKAVTRFRVLSQTNSVSVTDLPGVSGALANA
                     ISIKVKNNERRYNLKIKKRDYHHPTLGLNARFELVTEAGHPVVIDGVNMKGDTNSADN
                     SITFSNLPVGVYILKETVVPTGGYRPVNQLQDTKFEIRPDGSLQILESDSNMVTVDTS
                     QGATFEITIKNFKQFKFRLQKTDNRDENHLLNGATFKIYSDPNNDGVEDTEVASGVTT
                     NGLFETSLSFGYYILEETVAPTGYQLNPKKYRFQINHDGTTKLHNGDNQVTLAERVDG
                     DNVILFNMKNTKQTTHIKLAKRSYSDPTQRLVATFELRESDNPQATAVVKTTTTTGDE
                     VLFDNLAVGKTYILKEIVAPDGYQKIEKEFHINIGADGAISIQDGEDLVSLDNMDNHL
                     IIVKNLRKGEYPKTGGMGIIPYIALGGVMMFIAFAVEQRRKYTR"
     gene            complement(7384..10383)
                     /locus_tag="AC1PIW_001251"
     CDS             complement(7384..10383)
                     /locus_tag="AC1PIW_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HIP6606186.1"
                     /translation="MIEFRKKAVQLASLMSVFFLCTYSFTDAMYIMAESLSTDGASTI
                     RRTYIEDKKEDKDRLNIELVESLSSPKTIGQKITIDKQSLATQNFNEKGIVVITQKGL
                     ELKKDDLEKGWKLDESYNEKDLAITKSETEKRSLSNELDVLSKTVEELPVYGENYHSY
                     RLLPTTELDYSADNVSLTLSFTKVSEVIKGELVAVVDAEHIAYFKAEPSVFKEYSQVN
                     EKPSSTEDVNVVSPSQDPPVSETKENVPDNPESQGSSTVPESEQAVDALVEQRGVICI
                     KLTKSSSEQEEGIEDTENEAIEGATFEVRNVESENLVYTGQTDKDGLLTISNLPLGNY
                     AVIQKSTIDGYEISATKEVVELTVAQSRQTVSISNSPKNPLEGLMLNSILDSSLIPRS
                     ARVARSLLDTSLLDNPTVTGNANATTTTTVFGNKTTTITREESNIKYIFKPITISIPG
                     VYQSYSQDGVLKKKEVVVDSNTNTTKIIWEYTTTVGGVNSNITSIRNAFSITTDSGLG
                     EPKITSIMKDGVAITPNTTYYGNFDNFKSATDNLPVGNGTYVYTIETPVVIPSDNYSL
                     DYRSEVTVDAPKGSKLTYNGTSVTLTQKETRTLSTADTITLPAKNDGGPLGDLKVDTV
                     NTSNTNRTIGKYRDNDDKVIEWTSSQLNDTSTTQSFTFDVALDSSQAAHEYKVYIYEP
                     SNGTYTETKAEKVATPGNQITVDNVPAGAVALVKTVTNVKDEKVNHTISGAQLEALKG
                     DIKIQKNWEADSDKVDVTFTVNGGSLTNRKETLSANNTQITIANVDKFSGMRSTATKK
                     RIYYDVTEAVPSGYILSSAQTDWENLYYVFTNKKDNTTTPVFPPDTCGNYGVSSIDLV
                     SINYVMYKSGSKIWGGFDGSMKMNLKIPAFARAGDSFTLELPPELKLSHVANPNVAWS
                     TVSANGKVIAKVYHEKDNLIRFVLTTEAYSVQEYNGWFEIGVPTSNVIKINNRETTEL
                     YKTGVLPNLPEWYTTTTRNQTLIKRSR"
BASE COUNT         2911 a         2224 c         1841 g         3617 t
ORIGIN      
        1 ggataaagta aaatcccaaa tccaatcaaa aataccaagt aaaaggggat attacttttc
       61 ttttttttct ttgtagtttt tcccgatttt ttctgtatca taatccgaac ttcattattc
      121 ttccattgtc tttctactgc attgcaaagt taatactcaa tgaaaaacca aaagtagcct
      181 gagaaacgaa gccgaagaca gaactggagt tcatcaaggc tagttgacaa cggataattt
      241 tgattttcga agagtataaa atcccctaca ttctgctttt attataccat aatccaactt
      301 taaaacgaaa aatcccattg ccccagattc aacctgacaa tgggattttc cctctattct
      361 agtctgtcaa acgttatcgc ttagtataag ttaccaagcg ataacctagc aagaagagca
      421 aaataccggt agataaaact gcaatcacca ccacatttcc tgtatatgga atttcaattc
      481 caggaggtgt aacaggttta ttcttaatct taataatctc ggacacatgt gaatccgagg
      541 ttatttcaaa aggaatcgta tcttgagata aaatgtagcc agtaggggcc ttgacctctt
      601 ttaagtagta agtaccataa ggaagaggac ctaccacaaa ctgaccattc gaacttgacg
      661 tgacgtgata agaattgccg tcgactatca cttccttcaa ttctccgcct acttcctcaa
      721 gaaccgcaaa ttctgcccca gataaagggg cggtgctggt tgataccttt aaaaatggct
      781 tgctaccctt ttcttcgcta ggtctcctga ccttagcctc aatctttgta acacctctac
      841 ctacattcat caagaatggg ataacttcta gactcgtagg ctttcctact tgccgaacat
      901 aataccatcc cttagtcaat cctcgaaaag tggcttgacc ttctggagaa agagcttgag
      961 aagaaattgg ctcaccatag agactggtca actcagaccg acttttttga tataaagaat
     1021 ctgccaactg ttcagaagaa acgggttctt ctgaagcgat gtaccaagcc tccaaacccg
     1081 tcgaaacttg actggcatct attccattag ggaaaggaat atgaacctga acatcaaaag
     1141 aatcttgtgc ttggagtgaa agcgagggca atgataatac acatattata gcgacaaggc
     1201 aacatattat ttgtcttact gttttcatct gcccctcctt tctagtaata attaagcttc
     1261 ttcagattga cgctttttca tcgcgataaa ggcaaatacc atagtactca aacctacaat
     1321 tgtgaaaaca agtgtaccga taccacccgt ttgtggaatg gttacttttt tgtttttgat
     1381 ttgttggaag ttatctgctg tcaatgcatc tattgtaccc catgtatgtg ctcctacagt
     1441 gaattccaca ggcgaagtag ggagagcgaa tccttctgga gccttggttt caataagttc
     1501 atacttacct tctttaagac ctttgacttc aaacttacca tctgttgatg atacaaatgt
     1561 aaacgcacct tccttagtac ctacccaagt ccattgcatg ttcatctttt cgtaagcagt
     1621 gtcgcgagca tctttcttct gcttaatttc atcaatttta ggattgtctg ttgtggctga
     1681 tttaacagta gctaaataat cagcttccgc tgctttataa tctgcgattt cttttgcttg
     1741 tgttactgta tctttcaaag caagatatga ctcttcacca tctttacgaa cgatgaactc
     1801 agcacctaga agtttttcgc tactgtccaa atccttatca tctgtcttca caaatcgttt
     1861 accgtatgtg acaacttttg gatcttctgg tgtgagcgga gttggatttg gatttttctt
     1921 gttttctatt ctgatagtac ctgtatcacc atttgaatag ctaggaagtg agcctgaaac
     1981 gattgtttct tcaattacga tgtattcttt agcaccatca agaccagtta attttccagt
     2041 attaacacct gctgcaagtt taatttcacc tacacgtaca ccagttcctt tttcatatac
     2101 accgaataca acttctgttt ggtttacacc agtatcccat gttttgttaa caataatttc
     2161 accatctttt ggagttgttg attttggctc agagaattca cttggacggt taccgtaaat
     2221 caattttact tggttaggga tttcagtctc tactttcgct gagtcattta aagtagcctt
     2281 gtaagtcaag gtaaagttca cttcaccttc tcttgctttt tcttctaatt tcgcaagacc
     2341 gttattattc aatttcacca tgaatccacg tacagtttgt gtcaatgtgt agtctgtatc
     2401 tgctacaaag gttaagattt ttattatctg cgatttgtag tgagtttagt ttgaaatcaa
     2461 gacctgcaag catggtatct tcccaagcaa atgtcttata tgctgcgtcc ttagggactt
     2521 ttgttgtaat agtgtaacca atttcctgac cgatttcaac attttttata ccatcttgat
     2581 caacatttgt tctcttatca gtgaattctt tggtgatttc aggtttgttt tcagtattct
     2641 ttggatacac atgaagagga gttttggtat caaagtatcc agaaccatct gttttagcct
     2701 gtggcaattc aagcttgaat ggtacagctt tcataccagt aagttcattc tgattattgt
     2761 agtaaggtga attttcttta tcttcagtga agatataaat gccattagct agagtgaatg
     2821 ttgcaccgtt atcttgtgtc attgcagttt tatcggtttt tacatactta tttgtatctc
     2881 cagtaatacc aagttctgca cctgtcttag cttctggagc tactacatct gtctttttcc
     2941 atacgtcaaa ttttactccc gcaacttcca cggcagaatt accaaaataa ttcttcaaat
     3001 ctccgattga attaccatta tatttggata aattttcttc ataagtaaat ttattgaaat
     3061 cgtcattatt cattacgatt ttgtgaacaa taacggttgt gctagccgta tcttcggcat
     3121 ggactaagtg tttaatattt atcagtgaac cgagcaccgt taggaacaca gtaaacactg
     3181 aaaataattt cgtcaatttt ttcattgtat actcctattt ttatttctta ttttatttac
     3241 attaggaact atctagtata tttccgacgt tgctcaacgg caaaagcaat gaacatcatc
     3301 acccccccta atgcaatata tggaatgata cccataccac ctgttttcgg atattctcct
     3361 ttgcggaggt ttttcacaat aatcaggtga ttgtccatat tgtctagact gacgaggtcc
     3421 tctccatctt ggatagagat agccccatct gcgccaatat taatatggaa ttctttctca
     3481 atcttttgat aaccgtcagg ggctactatc tctttcaaga tataggtttt ccctactgct
     3541 aaattgtcaa agaggacttc atcccctgtt gtagtcgttg tttttacaac tgctgtcgct
     3601 tgaggattgt cactttctct tagttcaaac gttgctacaa gacgttgcgt aggatcgcta
     3661 taagaccgtt tagcaagttt gatatgggtt gtttgttttg tattcttcat gttaaagaga
     3721 ataacattgt caccatctac cctttcagct aaggtaacct gattatctcc attatgaagt
     3781 ttggtcgttc catcatggtt tatctggaaa cggtatttct taggatttaa ttgatatccc
     3841 gttggcgcaa cagtttcttc caaaatgtag taaccaaaac tcaaagatgt ttcaaagaga
     3901 ccatttgtcg tcacaccact agcaacttca gtatcctcaa caccatcgtt gtttggatcg
     3961 ctgtaaattt taaatgttgc accatttaga aggtgattct cgtcacgatt atctgttttt
     4021 tggagtctga acttaaattg cttgaagttc ttgatggtga tctcaaaggt agcaccttgt
     4081 gacgtatcaa ctgtcaccat attcgaatct gactctaaaa tttgtaaact accatctggt
     4141 cggatttcaa atttggtatc ttgtaattga ttaacagggc gatagcctcc cgtaggaaca
     4201 actgtctctt tcaaaatata gacgccaact ggcaagttcg agaaggtgat actgttgtca
     4261 gcactatttg tatccccctt catattaaca ccatcaatta caactggatg accagcctct
     4321 gtaacaagtt caaagcgcgc atttaaacca agagtcggat ggtgataatc acgttttttg
     4381 attttaagat tgtacctacg ctcgttattt ttcactttaa tgctaatcgc atttgcaaga
     4441 gcaccactaa caccaggtaa atctgttaca ctaaccgaat tagtttgact taaaactcta
     4501 aaacgtgtca cagctttctg ttgcgttacc gcataagctt gcgttgtttc gtataactcc
     4561 aacaataccg gtgcactcaa accgtcataa ccttctggtg cttgtgtctc tacaagaaca
     4621 tatttgccaa gcgctgtcgc agttaatcgt gtcaaactgc tttgacctgt aaatgttaag
     4681 gtttgtacga gattggtatc gtcaagagag gtacttgata cagttgcctc tgttgtacta
     4741 tcgtcaactt tatagagctt aaaggtaaca ggttttgaga tttgcttatt attcgcatct
     4801 actttctcga ttttgaactc tggattttcg atgttaccga ttttaataac attagcggca
     4861 gcatcacttc cacctgcttc aatcgtttgg gtagcatctc gaacacttac ctgcaattct
     4921 cggattccca accatctatt cgttcccgtc tgtagattcc gtacacgaat gtattgagca
     4981 tagatatcta cagtctgagt aaggtcggtc tcggtgaaat taccaattga ttgataggtg
     5041 ataccatccg gtgaatactc aagggcatag ctatccatct tgtcatggtc actaccgttt
     5101 gtggtatttt taccttgagc aaagcgaatt tgcgttacgc gctgaggcga acccaagttc
     5161 actccccacc attgtccctc attcaaagtc gaacttggga gttggtacaa ggcaccggtt
     5221 gtcaaatcac cgtccagcgc cttatcttct gtactagagc cataggtaga ggccatagca
     5281 atctctgcag attttatcac atgcaaacca cgtgctatgt gactacttat atccgagtca
     5341 gaatagaaac gcacgctgcc atctctatta acataaatcg tgtagttttc tgtcgatttg
     5401 acatagccag atggagcttg tgtttctgtc agagtataga ccccaggctg gaggttttca
     5461 aaaacaaagt cttggtttgc agcttgctgt ttgacgatag gggcaaagtt tacatcgtca
     5521 ttctttgtca aagtaaaggt catttgtcca gtgataaggt tggtcaaatc actaatccct
     5581 ttaacacgct tgctaacttt gaattttccg agtggatagt tcttgacttt tatcgtaatc
     5641 gtatctggcg agctcgtcgt cacatcaata ccttcaagat aatcactatt cacttctacg
     5701 ataccatctg cccgaatagt gaatggtacc aaacctgata agccaatata acccgatgga
     5761 gttttttctt ctttgatata gtaagttcct ggggtaaagt ttttaggaaa gctaaattct
     5821 ccattgtcaa agtttgcaat ttgcccatca attacattcc cttggctatc cgtaatacca
     5881 atacgtgtct gcacagttac tttctgtcca gtcagtgcat caattttctg cactttaact
     5941 ttataagttg gagggcggtt aataatacgc tcactagtac cagattcaaa actgatagtt
     6001 ccagtctcag ataccgtaaa tcgtgccgca gctgttgtta acttactata acctgctggc
     6061 gcactagttt cccaaagttc ataggttata ccaccttgca agtggtccac acggagcttg
     6121 ccttgactat ttgaagttaa gcctgttaga actcgtttgt agcctgtttg ggttatctga
     6181 cgaagctcaa acactgcatc tgcgagtggc tgaccttgct catttacctt ggtgaactca
     6241 gtactaaagt cctcgtcaac aacatttata ctaaagtttc cgttgacaac ttctaccgct
     6301 cccgtctgtg tattggaagt gtctgttaat ttacgtactt ttaactctcc attttcctca
     6361 taaacctcaa tatcaagagg aggaatcgtt ttataacccg caggagcttt tgtctccgtc
     6421 aaagtataaa tgccttttgc gaggttagag aaaataaact gacttgcagg aagaacatct
     6481 cctattgtga catcataatt tccaccattt ttactttgca gtctaaagac agctcctttc
     6541 aatggcttac tttcaccaaa ttcaaattta ttgatttgaa gtgtagttga tttagtatta
     6601 acaacttctg ttggcttatt gacatcgtat tttttcccat caatggtaat attttgatta
     6661 tcggttacaa caatagtgtg ctctgtcgga tctaagatat aaccagatgg tgcactagtt
     6721 tccttgaagg tgtaggtgcc gccgattatc tccgaaaact gaatatctcc attatcatct
     6781 gaaactgcgg ttcgatatgg ttcatttcct ttatataatg tgaaaatcgc acctgaaagt
     6841 ggaattttct gaccattttt aattccaacc tttttcagtt taaaaccata ccaatctaca
     6901 ggataagcac caccttgtga tactgtatct ccctcacgac gatagatatt agtggttctt
     6961 cctccaccca taactggact attccatggt acactcatac cattatagta cagaccattt
     7021 ttctgaaact ttttccgttc ttttagacgg actacaattg ctttattacc tagtttccaa
     7081 ccttcttgat aaaattcgat tgtatgagtg ctttcaaatc catcaaaagc cggataagaa
     7141 tttctctcag aataaactcg aacagatatt ccaatatccg tacctggtag gtaataatgg
     7201 tggatattcc cattatttga tgttccttta taagtgagct tttctaagct atccttagaa
     7261 taggacatag ccttggtacc atctgctaca tagagttcga tagattgagg aatactattt
     7321 gctacatttc catttggaaa caactctaaa cctggatgaa gaaaatcata aaattttctc
     7381 ctattaccta gacctcttaa tcaatgtctg attgcgagta gtcgttgtat accactcggg
     7441 aaggtttgga agcacccctg ttttatatag ttctgtagtc tctctattat tgattttgat
     7501 cacattactg gttggaactc caatctcaaa ccaaccattg tactcttgta cggaataagc
     7561 ctctgtagtc agtacaaagc gaattaaatt atctttttcg tgataaactt ttgctatcac
     7621 cttaccattt gcagacacag tggaccatgc aacattagga tttgcaacat gacttaactt
     7681 taactctggt ggcaattcta aagtaaatga atctcctgca cgcgcaaaag caggtatttt
     7741 caagttcatt ttcatcgaac cgtcaaaacc accccaaatt ttagaacctg atttatacat
     7801 gacatagttg atgcttacaa ggtctatact gcttacccca tagtttccgc aagtatctgg
     7861 tgggaatact ggtgtcgtgg tattatcctt tttatttgta aagacatagt aaagattttc
     7921 ccagtcagtc tgtgcgctag aaagaatata acctgatgga acagcttcgg tcacatcata
     7981 atatatcctc tttttagttg ctgtgcttct cataccagaa aacttatcaa cattagcaat
     8041 cgtaatttgt gtattatttg cgctaagtgt ttccttacga ttagttaaac ttcctccatt
     8101 aaccgtaaag gtcacgtcta ccttatcact atcagcctcc caattcttct ggatttttat
     8161 atcacctttc agcgcttcta gttgtgctcc tgaaatggtg tgattaactt tttcgtcttt
     8221 cacatttgtc actgtcttaa ccaaagcgac cgcacctgcc ggaacattgt caaccgtaat
     8281 ctggttccca ggcgtagcaa ccttttcagc tttagtttca gtataagttc cattacttgg
     8341 ttcatatata taaaccttat attcatgggc agcttgtgag ctatctagag caacatcaaa
     8401 tgtaaaactt tgggttgtcg acgtatcatt tagttgtgaa ctagtccatt cgatgacctt
     8461 atcgtcatta tcacgatact taccaattgt acgattggta ttggaagtat tgaccgtatc
     8521 tacttttaag tcccctagag gaccaccatc atttttagct ggaagcgtaa tagtatctgc
     8581 tgttgataag gtacgtgttt ccttctgagt taaggtaact gatgtgccat tataggtaag
     8641 cttgctgccc tttggagcat caacagttac ttccgaccga taatccaaac tatagttatc
     8701 acttggaatc accacaggag tctcaatcgt atagacatac gtcccgtttc caactggtaa
     8761 attatcggta gctgacttaa aattgtcgaa atttccgtag taagtcgtat tgggcgttat
     8821 tgcaactcca tccttcatga tagaagtaat tttaggttca cccaaaccag agtcagttgt
     8881 tattgagaag gcatttctaa tggaggtgat attgctattg acaccgccta ccgtagtagt
     8941 gtattcccaa atgatttttg ttgtattggt atttgagtca accactacct cttttttctt
     9001 caagacacca tcttgtgaat agctttgata aacacctgga attgaaattg taataggttt
     9061 aaaaatgtat tttatgtttg actcttcacg cgtgatggtt gttgttttat tcccgaatac
     9121 ggtggtcgtt gtagtggcat ttgcatttcc tgtcacagta ggattatcaa gtaatgaagt
     9181 atcaagtagc gaacgtgcta ctctcgctga acgcggtatc aaagatgaat ccaatattga
     9241 gttcagcata agtccttcta aaggattttt aggggagttg ctaattgaga cggtttgtct
     9301 tgattgagct actgttaact ccactacttc ttttgtggca gaaatctcat agccatctat
     9361 ggtagatttt tgaattaccg catagtttcc caacggaaga tttgaaatcg ttaggagacc
     9421 atctttatct gtctgaccag tataaacaag attttcactt tctacattac gcacctcaaa
     9481 tgtagcacct tcgatggctt cattttcagt atcctcaatg ccctcttctt gttcagaact
     9541 agattttgtt aatttgatac agataactcc tcgttgttcg accaaagcat ccaccgcttg
     9601 ctctgactct ggaactgttg aacttccttg tgattctgga ttatcaggta cattttcttt
     9661 tgtttcagag acgggtgggt cttgagaagg acttactaca ttgacatcct cagtagatga
     9721 aggcttttca ttaacttgag aatactcctt aaagacactc ggttcagctt taaaatatgc
     9781 gatatgttcc gcatctacta ccgctactaa ctccccctta ataacctcac tgaccttggt
     9841 aaaacttaaa gtcagactta cattatctgc cgaataatcc aattctgttg ttggcagcag
     9901 tctataggaa tggtagtttt ctccatacac tggaagttct tctacagtct ttgacaatac
     9961 atctaattca tttgaaagag agcgtttttc tgtctcactc tttgttatag ctaggtcctt
    10021 ctcgttatat gactcatcta gtttccaacc tttttctaaa tcatctttct ttaactctag
    10081 ccctttttga gtgataacta ctataccttt ttcgttaaaa ttctgtgtag ctaaactctg
    10141 cttatctatt gttatttttt gacctatagt ctttggacta gacaacgatt ctactaactc
    10201 aatatttaat ctatctttgt cctccttttt gtcttcgata taggtgcgtc ttattgttga
    10261 agcaccatcg gtcgacaaac tttctgccat gatatacatc gcatctgtaa aactatacgt
    10321 gcatagaaaa aaaactgaca ttaaactagc taattgaact gctttctttc taaattctat
    10381 catctcgtac tccttggttt gtgtctaaat ctgctaaagt tgagatacag ggacattatc
    10441 tttttcggtt ctaaatataa acaaaatcaa ctttaatact tattaagctt acatgaaatt
    10501 gatagttcaa ataccattat ttcaatgcac tataatataa atagcaatcg ccgtaagttc
    10561 cctgcaatct tcctctagtt ttcacttttt aca
//
LOCUS       DBNFCK010000062        10495 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.062, whole genome shotgun sequence.
ACCESSION   DBNFCK010000062 DBNFCK010000000
VERSION     DBNFCK010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10495
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.062"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            19..1311
                     /gene="purB"
                     /locus_tag="AC1PIW_001252"
     CDS             19..1311
                     /gene="purB"
                     /locus_tag="AC1PIW_001252"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIP6606187.1"
                     /translation="MIERYSRPEMAAIWSEENKYEAWLEVEILADEAWAELGEIPKED
                     VALIREKATFDIDRILEIEEETRHDVVAFTRAVSESLSEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTTIIADKAREHKYTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNMERFDVAAKGVEAGKISGAVGNFANIPPFVEEYVCGKLGIRPQEISTQVLPRDL
                     HAEYFSALALIATSIERMATEIRGLQKSEQREVEEYFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVVRGHLVTAFENVALWHERDISHSSAERIITPDTTILINYMLNRFGNIVKNL
                     TVFPENMKRNMESTFGLIYSQRVMLSLIEKGMTREEAYDLVQPKTAQSWDNQVDFKPL
                     LEADERVTAKLSQEEIDELFNPDYYAKRVDDIFERLGL"
     gene            2248..2976
                     /locus_tag="AC1PIW_001253"
     CDS             2248..2976
                     /locus_tag="AC1PIW_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HIP6606188.1"
                     /translation="MNMFRSIFKSVIHRRDVALFYAFAGLPILVPILSKFLVGVKAEY
                     TDNFLDFLGAALATQDGIVLPVLLLSLIISAVFRDEIDSGILFLYKDLNRTRLFNAKI
                     ISLVVMYASYVLLTVLTSAIAYFGFLNASGKVVSDDWSNVQSTFLSIFATISINVIGI
                     LLVSTVSIKAKSLQAVLAGVFWSLFTTTAPLLIGVRYVVPNGYAKMSLDQPLLAWSLV
                     VAITTFYTVATYLKGRSNFEKLEF"
     gene            2986..3564
                     /locus_tag="AC1PIW_001254"
     CDS             2986..3564
                     /locus_tag="AC1PIW_001254"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIP6606189.1"
                     /translation="MVVRYMRPVALSLALIVLASISYLLATSLVLFSSSQFLQLAYLF
                     SIMVGMPIGFILLPSMITKRYQLCQELSEVKFSWKSFVLLAIAIFLVNFWFIQSDEYV
                     NQFIIATCEEFLFRYLIYRILKSEYPTWLAMLVTSLLFGVLLHMNYSLLDNLIIRTPL
                     GLLFSVLATRFGLQYAIGGHWIYNLLVSRFPF"
     gene            3579..4007
                     /locus_tag="AC1PIW_001255"
     CDS             3579..4007
                     /locus_tag="AC1PIW_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msa family membrane protein"
                     /protein_id="HIP6606190.1"
                     /translation="MIFILLLSLFYICLTVASSQLLLSLSVPVLLLLTYLLPPIVNSI
                     CMGLQKDKSWKAWSAGIFPTMSMIFYAAFAYITSSNGQWEQFAQLHTVSDDVMSVELA
                     GELLVPSQMLFVAVLYYGTAIATYFIQQAGRKKEKGATYA"
     gene            4000..4872
                     /locus_tag="AC1PIW_001256"
     CDS             4000..4872
                     /locus_tag="AC1PIW_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6606191.1"
                     /translation="MLKINHLSKQFAGNEFYSLKDVSLEINKGEIVGLIGKNGAGKST
                     LMKLMAKSLKPSSGTITYKGTDIFSQDNLLADFGIMIDPVFYPEMTVMDNLKFYLDLH
                     GKKDLYPNIEKTLKLVELWDARNRKPKGFSFGMKQRTALAIALVAEPDFLILDEPFVG
                     LDPIGVQKLIDILKQWSSERQISMLISSHQLGELEALCSRYVYIESGELADAFEGKSH
                     PSVLVQLDTTKNLDSVKDLLNDHVVLEGEVLEISTATPTSELNQIFGVLTRQELIVKL
                     EVKENHLKEIFTKG"
     gene            4878..5651
                     /locus_tag="AC1PIW_001257"
     CDS             4878..5651
                     /locus_tag="AC1PIW_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606192.1"
                     /translation="MKQIFPAVFRTIWKRKETQVYLLFTLFPFIYLVTSFIEGSNFMQ
                     IHAGEGYKVSLVAFTNMMVSSADSFILPSLTLYFLAISVFRKEVDEHTMFLYRDLGRK
                     QIFWSKYLGLLATIVIFYGLFFVTSAFVHYVRVIHLPFGSPAVFEASLAESLSNVFCI
                     VAYLLKDILSISLATALCLYLKNITTMITGFLVTITMMILAIVGGPVAMLFPTSYMTL
                     ASEGLTGAGLAYLGAICVTIIYVLVFTKIAAKKFKNLEF"
     gene            5654..7366
                     /locus_tag="AC1PIW_001258"
     CDS             5654..7366
                     /locus_tag="AC1PIW_001258"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6606193.1"
                     /translation="MNKRLVGVLLKSLNKTMFLFGIVLSALGVGMGLFLPQFIGKLLD
                     QTYLSNLLTRPELLAGFILFFVSVYAVQALSNYFIGRSGSNALKQLQQYIYESLLTTS
                     VKDLDQYQSGDLASRLTNDMSVVLNFITVILPNFLMNGLMVMGSIYFLWTISPWLTGL
                     SLFLLPLLSMVMIPMNQRLEGYYSAYQEGLGQVSSRISHKFTTIRLMKAFQGEKHEQR
                     EMGKSFQYLSQTFEKMIGLSAVQHTLVSSLMTGFIILMLLIAGIEVTKGVMTMATLTT
                     FVLYMMQLIDPVTDIAASLNELTEFHAVSKRLVELLELNKEEQVDTELVDATSIQLEK
                     VRFSYHTDPILNGVSVRIPEGAHVAIVGPSGAGKSTIFALLMKYYQDYKGKIRIGQQC
                     LSDISTKEMRRLISFIPQDNTLFHGTIRENLLYGKNESVSEERIAYILKELGLSPLVA
                     ELEDGLDTRISENGTGLSEGQKQRFSIARALLLEHPIYLLDEATASLDTVTERVISKA
                     IDRLTAGKTRLTIAHRLHTVREADAILVLDKNGQVADYGHHQQLVERNHLYQDFLRGL
                     PQAS"
     gene            7468..7641
                     /locus_tag="AC1PIW_001259"
     CDS             7468..7641
                     /locus_tag="AC1PIW_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606194.1"
                     /translation="MKQTTYTLSLGGSDRENSRVSKMEEKFEKYMEGIAERLQQSGKK
                     RIDKWFVHVRIEE"
     gene            7936..8937
                     /gene="ruvB"
                     /locus_tag="AC1PIW_001260"
     CDS             7936..8937
                     /gene="ruvB"
                     /locus_tag="AC1PIW_001260"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIP6606195.1"
                     /translation="MTNRILDMEQMQDEEYVERTLRPQKLNEYIGQDKVKDQLKIFIE
                     AVKLRDEALDHTLLFGPPGLGKTTMAFVIANELGVNIKQTSGPVIEKAGDLVALLNDL
                     EPGDVLFIDEIHRMPMAVEEILYSAMEDFYIDIMIGAGEASRSVHLELPPFTLIGATT
                     RAGMLSNPLRARFGITGHMEYYELADLTEIVERTADIFDMEITHEAAIELARRSRGTP
                     RIANRLLKRVRDFAQIMGDGLIDDSITDKALTMLDVDREGLDYVDQKILRTMIEMYGG
                     GPVGLNTLSVNIAEERETVEDMYEPYLIQQGFLMRTRTGRVATAKAYEHLGYPYTEK"
     gene            8937..9683
                     /locus_tag="AC1PIW_001261"
     CDS             8937..9683
                     /locus_tag="AC1PIW_001261"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6606196.1"
                     /translation="MAEQMRQAARLFGDWPETIIWTCLEGVMGDIYVDDSQLPQSALA
                     LYGRQSFFGFLAGQPHRDLLKICEGKDMILVPQNQNWSDLIEGTYGDGIRSFTRYATK
                     KDTEFDLGHLQKLVDDLPESFDMKRIDRNLYEACLVEEWSRDLVGNYIDVEQFLDLGL
                     GYVILHKGQVVSGASSYASYSAGIEIEVDTREDYRGLGLAKACAAQLILACLDRGLYP
                     SWDAHTLTSLKLAEKLGYQLDKPYQAYEWR"
     gene            9685..10320
                     /locus_tag="AC1PIW_001262"
     CDS             9685..10320
                     /locus_tag="AC1PIW_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HIP6606197.1"
                     /translation="MTPTFIWDLDGTLLDSYEAILAGIQETYQEYVLPFDRAEVRKFI
                     LRYSVKDLLVRDADKYGLDSDELNRVRATSLKEKNTQIPLMAGAVEILNWTAEQGIQN
                     FVYTHKSDNAFQVLEDLGVRHHFTEILTSDSGFARKPSPEALLFLIEKYGLDKENTYY
                     IGDRLLDVETAVNAGIHSINLQIDGVEQNWKIVSLLDIKQILTDKSSHNNL"
BASE COUNT         2910 a         1931 c         2499 g         3155 t
ORIGIN      
        1 aagtaaggag caaataaaat gattgaacgt tattcccgcc cagagatggc ggctatttgg
       61 agtgaagaga acaagtacga ggcttggttg gaggtggaaa tcctcgctga tgaggcttgg
      121 gctgagttgg gtgagattcc caaggaagat gtggccttga ttcgtgagaa ggcgactttt
      181 gacatcgacc gtattttaga gattgaagag gaaactcgtc acgatgtggt ggctttcacg
      241 cgtgcggttt cggaaagtct tagtgaggaa cgtaagtggg tccactatgg tctgacttcg
      301 accgacgtgg tggatacggc ttacggctac ctatacaagc aggccaacga catcatccgt
      361 cgtgaccttg aaaactttac cactatcatc gccgacaagg ctcgtgagca caagtacacg
      421 attatgatgg gccggaccca cggtgtccat gcggagccaa cgaccttcgg tcttaaatta
      481 gcaacttggt atagcgaaat gaaacgcaac atggagcgtt ttgatgtggc ggccaagggc
      541 gttgaggctg gtaaaatttc aggtgcggtt ggaaactttg ccaatattcc tccatttgtg
      601 gaagaatatg tttgtggcaa attgggtatt cgtccgcagg aaatttcgac ccaggttctt
      661 cctcgtgacc ttcacgcaga atatttctca gccctagcct tgattgcaac gtctatcgag
      721 cgtatggcga cagagatccg tgggctacaa aaatctgaac aacgtgaagt cgaagagtat
      781 ttcgccaaag gccaaaaggg tagctctgct atgccccata aacgcaaccc tatcggctct
      841 gaaaatatga ccggtctggc tcgtgtggtg cgtggtcact tggtgacggc ttttgagaat
      901 gtggctctct ggcacgaacg cgatatttcc cactcgtcag cggagcggat tatcacgccg
      961 gatacgacca ttctcatcaa ctatatgctc aaccgttttg gcaatatcgt taagaacttg
     1021 acggtcttcc ctgaaaatat gaaacgcaat atggagtcaa cctttggctt gatttacagt
     1081 cagcgtgtca tgctcagctt gattgagaaa ggcatgacac gtgaggaagc ctatgatttg
     1141 gtgcaaccaa aaacggcgca atcatgggac aatcaagtgg acttcaagcc ccttctcgaa
     1201 gcggatgagc gtgtgactgc caaactcagt caggaagaaa tcgatgaact cttcaacccc
     1261 gactactatg ctaagcgggt ggatgacatc tttgaacgac ttggattata aaaaaatcaa
     1321 aattctttgg attttccaga gaattttttt gtttacttgc gaataaatag gtgaagggga
     1381 aaacaagctc cctccgctag acaatcattc gtaagaggat gagcagaaag tccagcctag
     1441 cttctcagag ttcgtatcaa catttcagcg cagtggttga ttggcagatt cgttcgcgtt
     1501 tcacgctcca aatctgacct aatcaactgt gcggggtggg aagacgaact ctttagattg
     1561 gtcgagttct gtcccactcc caaaatttta cgaatagata agtgtagggg aagcaagctc
     1621 acaacttatt caaaaaaata agattttttc aaaaaaaaga aaacgatttg cgaataagta
     1681 tagtgagagg gaaaatcccc ctcaaggaga tacacacttg aaaaaaataa atcattttca
     1741 aaatgattta cgaataaaca agtgtaggaa aaatgaaagg agaagaagga tgaaaaagaa
     1801 gttcaagatt gtaaacaaac gtacaacgat tgacctcggg attattaagt atagctaccg
     1861 tcgtggtcgt cataccttgt aaatcaatca gtaaatcaaa ttgttaaagt aataggagga
     1921 aatagaaatg aacaaatttt ttaagaaaaa taaacgtgtg acaatcgatc taggtattat
     1981 caaatacacc tatcgtcgtg gtcgtcatac tttgtaaaca gattagtaaa tcatattatt
     2041 tgtaaatttt taaagaaaaa ggagtaaaaa tgaaacaatt atttaagaag aataagcgca
     2101 tcactattga tttaggtatc atcaaataca cttatcgccg cggtcgtcac actttgtaaa
     2161 aagaacttga tagctaattt gatttagcta tcaataagat gggaatttgt tacagttcct
     2221 atcttattga taaaaagggg aaacaatatg aatatgttta ggtcaatttt taagtcggtg
     2281 atacatcgaa gggatgttgc tttgttctat gcttttgcgg gtttgccaat cttagttcct
     2341 atattgtcca aatttttggt aggcgtaaag gctgaatata cggataattt tttagatttt
     2401 ctaggagcag cattggcaac acaggacggg atagtgttac ctgtattact gttatccttg
     2461 attatttcag cagtctttag ggacgagatt gatagtggca ttctattttt atataaagat
     2521 ctgaatagaa cccgactttt taatgcaaaa atcattagtt tagtggtgat gtatgcctct
     2581 tatgtccttc taacagtgct gacaagtgcg attgcttatt ttggcttctt gaatgcttct
     2641 ggaaaagtag tatcggatga ttggagtaat gtacagtcca ctttcctatc tatttttgca
     2701 acaatttcaa tcaatgttat cggtatttta ttggtttcta cggtttctat caaagccaaa
     2761 tccttacagg cagttttagc aggagttttt tggtcactgt ttacaacaac tgcaccactt
     2821 ctcattggag ttcgttatgt tgtgccgaat ggctatgcta agatgagtct tgatcagcca
     2881 ctgcttgcat ggtccttggt agtagctata acgacatttt atactgttgc tacctatctc
     2941 aaaggtcgtt cgaactttga aaagctagaa ttttagaggt gttttatggt agtgcgctat
     3001 atgaggccag ttgctttatc gcttgctttg attgttttag cctccattag ttatcttttg
     3061 gcaacatctt tggttttgtt tagctctagt caatttctac agctagccta cctattttct
     3121 attatggttg gaatgccaat agggtttatt ctacttccat ctatgataac caagcgttat
     3181 cagctttgtc aggagctatc ggaagtgaag ttcagttgga aaagctttgt tttgctggct
     3241 attgcaattt tcttggtcaa tttctggttt attcagagtg atgagtatgt gaatcaattt
     3301 attattgcga cttgtgaaga attcttgttc cgttatctta tctatcgtat tctaaaaagt
     3361 gaatatccta cttggctagc tatgttggtc acttctctct tgtttggagt tttgttgcat
     3421 atgaattatt ccttgctgga taatctaatc atccgaactc cactaggttt attgttttct
     3481 gtgttagcta ctcgttttgg cttgcagtat gctatcggag gacattggat ctacaatctg
     3541 ttggtttcga gatttccatt ttaattgtga gggtcatgat gatatttatt ctattattga
     3601 gtctatttta tatttgttta acagttgcta gttcgcaact attgctaagt ctctctgttc
     3661 ctgttttatt gttgttgact tatttgctac cgcccatcgt caattcaatc tgtatgggat
     3721 tacaaaaaga taagtcttgg aaagcgtggt cagcaggcat tttcccaaca atgtcaatga
     3781 tattttatgc cgcttttgcc tacattacga gtagcaatgg tcagtgggag cagtttgctc
     3841 aactgcatac agtttcagat gatgtcatga gtgtcgagct ggcaggagaa ttgttagttc
     3901 catcacaaat gctatttgtt gccgtactat attatggtac agctattgcc acttatttta
     3961 ttcagcaggc aggtcgtaag aaagaaaaag gagctactta tgcttaaaat caatcattta
     4021 tcaaaacagt ttgcaggaaa tgaattttac tcactaaaag atgtgagttt ggagatcaat
     4081 aagggcgaga ttgttggtct gattggaaaa aacggtgctg gaaaatccac gcttatgaaa
     4141 ctcatggcca agtccctaaa accaagttcg ggaaccatta cttataaggg tacggatatt
     4201 tttagccagg ataatttgtt agcagacttt gggattatga ttgatccagt attctatcca
     4261 gaaatgactg ttatggacaa tctcaagttt tatttggacc tgcatgggaa aaaagacctc
     4321 tatccaaata ttgagaagac tttgaaattg gtagagttat gggatgctcg caatcggaaa
     4381 cccaagggat tctcgttcgg tatgaaacaa cggacagctt tggcgattgc cttggttgcg
     4441 gagccagatt tcctgatctt agatgagcct tttgtgggct tggacccgat tggtgttcaa
     4501 aaattgattg atatcttgaa gcagtggtcc agtgagcgac aaatttccat gctgatttcg
     4561 agtcaccaac taggtgagct ggaggccttg tgcagccgct atgtctatat cgaatcggga
     4621 gaattggcag atgcttttga aggcaaatct catccaagtg tgctcgtgca gttggacacg
     4681 actaaaaact tagattctgt gaaggacttg ctcaatgacc atgtggtgct agagggggaa
     4741 gtgttagaga tttccactgc cacacctaca agtgagctaa atcagatttt tggtgtgttg
     4801 actcgtcaag aattgattgt gaaactagaa gtgaaagaga atcacttgaa agaaatcttt
     4861 acgaaagggt agtgagtatg aagcaaattt ttccagctgt ctttagaacg atttggaagc
     4921 gaaaggagac gcaggtttat ctcctattca ctttgtttcc ttttatttat ctagttactt
     4981 cgtttattga agggtcgaat tttatgcaga ttcatgctgg tgagggctat aaagtcagcc
     5041 ttgttgcctt tacgaatatg atggtgagct cagcagatag tttcattctt cctagtttga
     5101 ccctgtattt cttggctata tctgtcttta ggaaagaagt agatgaacat accatgtttt
     5161 tataccggga tcttggacgg aagcagattt tctggtctaa atatttgggt ctcttagcaa
     5221 caatcgtcat tttctatggt ctcttctttg taacctctgc atttgtacat tatgttcggg
     5281 taatccatct accttttggt agtccagctg tttttgaagc tagtttggca gagagcttga
     5341 gtaatgtatt ttgtattgtc gcttatttgc taaaagatat tttatcgatt agtttggcaa
     5401 ctgctctttg tttgtatctg aaaaatatta ccacaatgat cacaggattt ttggtgacga
     5461 ttacgatgat gattttagca attgttggcg gtccagttgc gatgcttttt ccaactagtt
     5521 atatgacttt ggcgagcgaa ggcctgacag gagcggggct tgcttattta ggagctatct
     5581 gtgtcacaat tatttacgta ctcgtattta ctaaaattgc tgcgaaaaaa ttcaagaatt
     5641 tggagttcta gttatgaata aaagactcgt cggagtgttg ttgaagtcgc tcaataaaac
     5701 gatgttcttg tttgggatag ttctgagtgc acttggggtt ggaatgggtt tgttcctgcc
     5761 ccagtttatt ggtaaattgc tagatcaaac ctatttgagc aatctattaa cacggccaga
     5821 attgctggca ggtttcatcc ttttctttgt atcggtctat gcagtacagg ctctatctaa
     5881 ttatttcata gggcgtagtg gtagtaatgc actcaaacag ttgcaacaat atatttatga
     5941 atccttgttg accacatctg taaaggactt ggatcagtac caatcaggtg atcttgctag
     6001 tcgtttgact aatgatatgt cggttgttct gaacttcatc accgttattc ttccaaattt
     6061 cttgatgaat ggtttgatgg ttatggggtc aatttatttt ctgtggacca tcagtccgtg
     6121 gctcacgggt cttagtctct tcttgcttcc tttgttgtca atggttatga ttccgatgaa
     6181 tcagcggttg gaaggttact actctgctta ccaagaggga ttagggcagg tttctagtag
     6241 aattagtcat aaatttacaa cgattcgttt gatgaaggct tttcagggag aaaagcatga
     6301 acagcgagag atgggcaagt cttttcagta tctgtcacaa acctttgaga aaatgattgg
     6361 actgtcagcg gtgcaacata ccttggttag tagcttaatg acgggattta ttatcctgat
     6421 gttgcttata gcggggatag aagtaaccaa gggagtgatg accatggcta cattgacaac
     6481 ctttgtattg tacatgatgc aactgattga tccagtcaca gacattgctg cttccctaaa
     6541 tgaactgacg gagtttcatg cagtgtctaa acgtttggta gaattgctgg aactgaataa
     6601 ggaagaacag gtagatacag agttggttga tgctactagt atccagttgg aaaaagttcg
     6661 gttttcctat catacagatc cgattttgaa tggagtatcg gttagaattc ctgagggagc
     6721 tcacgtggcg attgttgggc caagcggagc cggaaagtca acgatttttg ccttgctgat
     6781 gaaatattat caagactata aggggaagat tcggattggt caacaatgtt taagtgatat
     6841 ttcaaccaag gagatgagaa gattgatttc cttcatacct caggacaata ctctctttca
     6901 tgggacgatt agagaaaatc tattgtatgg aaagaacgag tctgtgtctg aagagcgtat
     6961 tgcttatatt ctcaaggagt tgggattgtc tcctttagta gctgagttgg aagatggttt
     7021 agatactcgg atttcagaaa atgggacagg cttgtcggaa ggccaaaaac aacggttcag
     7081 cattgcaaga gccttactgc tagaacatcc gatttatttg ctagatgagg cgacagcaag
     7141 tttagatact gtcacagaac gtgtcattag caaagcgatt gaccgattaa cagctggcaa
     7201 aacaaggcta accattgctc accgtttgca tacggtgcgt gaggcagatg ctattttggt
     7261 tttagataaa aatggacaag tagctgacta cggtcatcac cagcagttag tggagagaaa
     7321 tcatctgtat caagatttct taagaggctt accgcaagcc agttagtgaa tgggagtcaa
     7381 tggaccgacc agtctaagtc tagggttacc taataataaa atggaatact gatgattact
     7441 tgtacaatga atagatagga gaagagaatg aaacaaacaa catatacctt atctttggga
     7501 ggaagtgaca gagagaactc cagagtttcg aagatggaag aaaagtttga aaaatatatg
     7561 gagggaatcg cagagcggtt acagcaatct gggaagaagc gaattgacaa atggtttgtt
     7621 catgtgcgaa ttgaggagta aagtagctat gaccaaaatt cacagacgtt tttataaata
     7681 gtgaaaaata gaatgaattc tcttttagtt tcccttaagt ttggcgagtt atactttatt
     7741 caagctagaa atagcgagac ttttactgaa atagtttcaa tatcgtcatc cgtaagagct
     7801 aaagctcaaa cggctggcat atcttttcaa tccttatctc taagtcgcaa gcgacaagga
     7861 ctagggatta tataatctcc tgagaatact ggactcactg agtctggtat tttcatttta
     7921 tgctataatg gtttcatgac aaatcgaatt ttagatatgg aacaaatgca ggacgaggaa
     7981 tatgtcgagc gtaccctgcg tccgcagaaa ttaaacgaat acatcggtca ggacaaggtt
     8041 aaggaccagc tcaaaatctt tatcgaggca gtcaagctcc gtgatgaagc cttggaccat
     8101 acccttctgt ttggacctcc aggtttgggg aagaccacca tggcctttgt catcgccaac
     8161 gaactgggcg ttaatatcaa gcagactagt ggtcctgtta ttgaaaaagc aggtgacttg
     8221 gtggcccttc tcaacgactt ggagcctggc gacgtcctct ttatcgatga aatccaccgt
     8281 atgcccatgg cggtcgagga gattctctac tcagccatgg aagacttcta catcgacatc
     8341 atgattgggg ctggggaggc cagtcgctcc gtgcatttgg agttgccgcc ttttaccctg
     8401 attggagcga ccactcgtgc gggtatgctg tccaatcccc tgcgggcccg atttgggatt
     8461 accggtcaca tggagtatta cgaactggct gatttgacgg agattgtcga gcggacggcg
     8521 gatatctttg acatggagat tactcatgaa gctgctattg agctggctcg tcggtctcgt
     8581 ggaactcccc gtatcgccaa ccgtctgctc aagcgggtgc gggatttcgc acagattatg
     8641 ggcgacggcc tgattgacga tagcattacg gataaggccc tgactatgct ggatgtggac
     8701 cgagaggggc tggactatgt ggaccagaag attctccgca ccatgattga gatgtacggt
     8761 ggcggtcccg tcggcctcaa caccctgtcg gtcaatattg ccgaggagcg tgagacggtg
     8821 gaggacatgt acgaacctta cctgattcag cagggcttcc tcatgcggac gcggacaggg
     8881 cgggttgcga cagccaaggc ttacgagcac ttgggttatc cctatacgga aaaataatgg
     8941 cagagcagat gagacaagca gctcgtttat ttggagactg gcctgagacg attatctgga
     9001 cctgtttaga aggtgtcatg ggagacattt atgttgatga cagccagttg ccccagtcag
     9061 ccctagcact ctacggacgg cagagcttct ttggtttttt agctggccag ccacatcgag
     9121 acttgctaaa aatctgcgaa ggtaaggata tgattctagt ccctcaaaac caaaactggt
     9181 ctgacttgat agaagggact tacggagacg ggattcggtc ctttacccgc tatgctacga
     9241 aaaaggacac tgagtttgac cttgggcatt tacagaaact ggttgatgac ttgcctgaaa
     9301 gctttgatat gaaacggatt gaccgtaatc tgtacgaggc ttgtctggta gaggaatggt
     9361 cacgggattt ggtgggaaat tatatagatg tggaacaatt tttggacttg gggctgggct
     9421 atgtcatctt gcacaagggg caggtggtct caggggcttc gtcctatgcc agctattcag
     9481 ctgggattga gatagaagtg gataccaggg aagactatcg aggtctgggt ttggcaaaag
     9541 cctgtgcggc tcagttaatt ttagcttgtt tagaccgcgg tctctatcct agctgggatg
     9601 cccacacctt gacatccttg aaactggctg aaaagctggg ctaccaattg gacaagccct
     9661 atcaagcata cgaatggaga taagatgaca ccgacattta tttgggattt ggacggaacg
     9721 ctgttggatt cctatgaagc gattttggca gggattcaag aaacctatca agaatatgtt
     9781 cttccctttg accgtgcaga agtaagaaag tttatccttc gctattctgt caaggatttg
     9841 ctggtgcgtg atgcggacaa gtacggtctg gatagcgatg agctcaatcg tgtgcgtgcg
     9901 acttccttga aggaaaagaa tacgcagatt cccttgatgg ctggtgcggt ggaaatctta
     9961 aactggactg cggaacaagg tattcaaaac tttgtctata cccacaagag tgacaatgct
    10021 tttcaggtct tggaggattt gggtgtccgt caccatttca cagaaatctt gaccagcgat
    10081 tctggttttg cccgcaaacc aagtccagaa gctctgctat ttctcatcga aaaatacgga
    10141 ctggacaagg aaaataccta ctatatcggt gaccgtttgc tcgatgtaga aacagctgtc
    10201 aacgcaggaa ttcacagtat caacctgcaa attgatggtg tggagcagaa ttggaagatt
    10261 gtttctttat tggacataaa acaaatatta acagataaat caagtcataa caacctgtga
    10321 aaacgggtgg cttgtacacc agctataagc tgtttctctc cagcgacgtc taaagatgtc
    10381 cgctgaactt cgttcaggtt agtgctatga ttacttgact aatgcccgta acatcgggct
    10441 tttgttttgt tatggcttca agctataata aaaatgagtc caaccgtctg tttta
//
LOCUS       DBNFCK010000063        10138 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.063, whole genome shotgun sequence.
ACCESSION   DBNFCK010000063 DBNFCK010000000
VERSION     DBNFCK010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10138
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.063"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..370
                     /gene="nrdI"
                     /locus_tag="AC1PIW_001263"
     CDS             <1..370
                     /gene="nrdI"
                     /locus_tag="AC1PIW_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HIP6606198.1"
                     /translation="FQTDWEIELVHVKDLVKQAIPFYQLDAPFVAFLPTYLEGGNGVD
                     NGDVEILTNPLGDFIALGTNAELCLGVVGSGNRNFNNQYCLTAKQYAERFNFPVIDTF
                     ELRGMQNDIERIGRKIMELM"
     gene            complement(524..2575)
                     /gene="pulA"
                     /locus_tag="AC1PIW_001264"
     CDS             complement(524..2575)
                     /gene="pulA"
                     /locus_tag="AC1PIW_001264"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HIP6606199.1"
                     /translation="MALRQFQASLDDVATIRLVMKKRFDSEHMSFSLESNDATSQLFI
                     HSCLEVDNLVIYYLTSLHALTLDKDYTIYDQDRNKTELGYGHIVRSTLFEQSYTYNGQ
                     DLGATYRPEATTFKLWAPISKQVFLVLEGNPHAMTKGAKGVWEITVEGDLEAHSYHYL
                     HKVNGEWISVHDPYALSSTANSGDSYVINPDKLHKIRRAKTQRPISQAIIYEMSVRDF
                     SWQKEAGFKHRSQFLGLTESPTMDGMKLGMDYIRELGVTHIQLLPVYDFGSVDENKPQ
                     AVYNWGYDPMQYNLPEGSYSSQPNDPYARILELQEAIQAYHDADISVIMDVVYNHVYH
                     AEKYAFELIVPGYFYRYDDHGMRTDGTFCGNDVASERSMVRNYIKQSLRQWLELYGFD
                     GFRFDLMGILDSETINQIQTELSAIHPNVYLYGEGWKMATGLEFEKLAHQYNAEHLPE
                     ISFFNDDYRDTFKKLLLNPNRLIDKQLHEKVQHLLTGSRLSHFLTPQQSLNYIECHDN
                     ATAFDYFHIENPNWTPQQQKRAASFGLQLILISQGMAFIHSGQEFFRTKDEIDNTYNI
                     PDRINRLDWTRAIRYKEHIEFIQELITFRKNNPILSQDNYESIQETCDFYWLTEYVLR
                     YQVTSEEKTIQFIINFADSDFTYEREKDKAVLYNYPPVEERDQQEITIAGQSICILED
                     K"
     gene            complement(2599..3582)
                     /locus_tag="AC1PIW_001265"
     CDS             complement(2599..3582)
                     /locus_tag="AC1PIW_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIP6606200.1"
                     /translation="MRATIKDVAKLAGVSPSTVTRVIQNSSAISQKTKDLVRKAMADL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDVFYQNPFFPTALRGISQVAADHNYAIQIST
                     GKNEEQRLEAISQMVYGKRVDGLIFLYSKPDDPLVQLAIQHKFPFLILGKADSPFISL
                     VDNDNIQAGFEATNYFINKGYKNIAFVAGNKELVVSQDRYTGYKNALKSHNIPLDENK
                     VKFVSGFLLEDSAYKISKKLLKQDLDAIVTTDTSVAEGVVKYLNEVGVKLPIISFDSV
                     KPKLDIEAYVDVHAIKLGRVAFNTLHQIINDNKEDKQVCYRRVIPHTITEL"
     gene            complement(3592..4302)
                     /locus_tag="AC1PIW_001266"
     CDS             complement(3592..4302)
                     /locus_tag="AC1PIW_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606201.1"
                     /translation="MNLWQRLFTTLFLVALLLIPSSLQTVNLETYPLENFVEGIYAPL
                     TDQVVDDLRQHAVIENNHLTYTGNTNWEQVTFGDTVSSSSDFTYQFANQSLTIRKGSD
                     ILSELTYEGLNTDDFASKETLTATISKKWYQANRAAVGLSITLISTFILTTNLLFILL
                     GASFFLFLTKKSRFFHFETFAECYNFALNCLGLPTLIACLIGLFGQPVTTLLTIQNIL
                     FVLCLLWVFFKTKFRDEI"
     gene            complement(4434..5276)
                     /locus_tag="AC1PIW_001267"
     CDS             complement(4434..5276)
                     /locus_tag="AC1PIW_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HIP6606202.1"
                     /translation="MKVSVKTRRILNQTLTYLYLIVLSVIIIYPLLITIMSAFKSGNV
                     VAFKLDSNINFTLDNFSKLFSETLYGTWYFNTLIIALLTMVIQTSIVVLAGYAYSRYN
                     FIARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNQSWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPISLDESAKLDGAGHFRRFWQIVLPLVRPMIAVQALWAFMGPFGDYILSKF
                     LLREKEFYTVAVGLQTFISDVKNMKIAYFAAGAILIALPISILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(5278..6573)
                     /locus_tag="AC1PIW_001268"
     CDS             complement(5278..6573)
                     /locus_tag="AC1PIW_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003027817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIP6606203.1"
                     /translation="MTMKQNPGKALLLSLIPGLGQIYNKQKAKGYIFLGVTLAFLVYF
                     IAIAAGELGNLITLGSVRGQDNSLFMLIRGSFHLIITVVYLAFYALNLKDARDTAKRW
                     NSRMPVATTLKEMVKGIYENGFPYLLIIPSYIAMTFAIIFPVVVTLFIAFTNYDFKHL
                     PPSALLDWIGITNFTNIWKLSTFRTAFGSVLGWTIIWALCASTAQIVIGILTAIIANQ
                     PFIKGKRIFGVIFLLPWAVPAFVTILTFSNMFNDSVGAINTQVLPFLSKFLPFIDNFL
                     IPWKTDPFWTKVALIMMQAWLGFPYIYVLTLGILQSIPNDLYEAAYIDGASAIQKFRN
                     ITLPMILAVAAPTLISQYTFNFNNFSIIYLFNNGGPGSVGGGAGSTDILISWIYKLTT
                     GTSPQYSMAAAVTLIISMIVISISMIAFKKLNAFEMEDV"
     gene            complement(6786..8045)
                     /locus_tag="AC1PIW_001269"
     CDS             complement(6786..8045)
                     /locus_tag="AC1PIW_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIP6606204.1"
                     /translation="MKHNLLKSVALLAASTAVLAACSNSGSSTEASKSAEGSKELTVY
                     VDQGYESYINDVKAGFEKENGVSVTVKTGDALTGLDNLSLDNQSGSAPDVMMAPYDRV
                     GSLGSEGQLSELTLADDSKADDTTTALVTNGGKVYGSPAVIETLVLYYNKDLLTEAPK
                     TFAELETLAKDSKYAFAGEEGKTSAFLADWTNFYYTYGLLSGYGGYVFGENGTNPKDI
                     GLANEGAIKAIEYAKTWYEKWPQGLQDGTAANNLINTQFTDGKAAAIIEGPWKAASYK
                     EAGVNYGVATIPTLVNGKNYSAFGGGKAWVVPAGAKNQEMAQKFVDFLTATDQQKALY
                     DATNEVPANTEAREYAVSKKDELTTAVINQFASAQPMPNISEMGSVWTPAGNMLFEAA
                     SGSKDAKTAATDAVKAIADEIAQKHSN"
     gene            8348..9865
                     /gene="malQ"
                     /locus_tag="AC1PIW_001270"
     CDS             8348..9865
                     /gene="malQ"
                     /locus_tag="AC1PIW_001270"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HIP6606205.1"
                     /translation="MKVRQSGVLMHISSLPGKYGIGSMGQSAYEFVDFLVRTKQRYWQ
                     ILPLGTTSYGDSPYQSFSAFAGNTHFIDFDILIDKGWLAAEDLHGVDFGQNPTEVDYA
                     AIFYARRPLLEKVVANMKAQGLPEDYWMFLGENDFWIHTFAEYMAIKEHFDLKPWTEW
                     EDQGARLRYHDTLEYYRHLLADRIDYHRITQYLFFSQWKALKAYANAKGVEFVGDMPI
                     YIAADSADMWANPHFFKTDEEGKPSVVAGCPPDAFSEIGQLWGNPIYDWEAMKHDGFT
                     WWVARLRESFKIYDMVRIDHFIGFASFWEIPAGEETAVNGYRVEAPGFELFDTIKREL
                     GDLNIIAEDLGSVTDKVIQLRDTTGFPGMKVLQFAFDPEGDSIEMPHNHPNNVVAYTG
                     THDNDTILGWYENETTKESRAFLDKYSNRREGETVSHALFRLLFGSPAFMAVATMQDL
                     LGLDGSARMNLPNTLGGNWTWRMTADQLTPEIEAELLDLTETYRRVNVHLVDEKS"
     gene            complement(9908..10090)
                     /locus_tag="AC1PIW_001271"
     CDS             complement(9908..10090)
                     /locus_tag="AC1PIW_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606206.1"
                     /translation="MKKLPTYIGVIIFVVTLLINWLLPSLAKVLNTFFIWTIVACLLY
                     TIYYWIRYWLYVLQQR"
BASE COUNT         2914 a         1854 c         2345 g         3025 t
ORIGIN      
        1 atttcaaact gactgggaga ttgagctagt tcatgtcaaa gatttggtca agcaggctat
       61 tcctttctat cagctagatg caccctttgt cgccttttta ccaacctact tagaaggtgg
      121 aaatggagtg gacaacggag atgtggaaat tctgaccaat cctcttggag attttatcgc
      181 tttggggacc aatgcagagc tctgtctggg tgtcgttggc tcaggtaacc gcaattttaa
      241 caaccaatat tgtctgactg ccaagcaata tgctgagcga tttaattttc ctgttataga
      301 cacctttgaa ctcagaggga tgcagaatga cattgaacgc attggtcgga agataatgga
      361 attgatgtaa agagcctcaa ctcagcttta tgctgggttg tttttttggt cacacaaaaa
      421 aaactcacca gagtgactag tgagttttaa ggagattatg aaaaatattt aggattgact
      481 atagtatacc tcagactata tcgtgtttca tcggtctaaa gtcctatttg tcttctaaaa
      541 tgcaaatact ctgtcctgca atcgtgattt cttgctgatc acgctcttcg actggaggat
      601 agttgtagag aactgcctta tctttttcac gttcataggt aaagtcgcta tctgcaaagt
      661 tgatgataaa ttgaatggtc ttttcttcac ttgtcacttg atagcgaaga acatattcag
      721 ttaaccagta gaaatcacaa gtttcttgga tactttcgta gttatcctgg ctgagtattg
      781 gattattttt gcggaaggta attaattcct gaatgaactc gatatgttcc ttataacgaa
      841 tagctcgtgt ccagtccaga cggttgatac ggtcagggat attgtaagta ttatctattt
      901 catcttttgt acggaaaaat tcctgtccgc tgtgaatgaa ggccataccc tgagagatta
      961 agatgagctg gagaccaaaa cttgcagcac gtttctgctg ttgaggtgtc cagtttggat
     1021 tctcaatatg gaaatagtca aatgctgtag cattatcatg gcattcgatg tagttgaggg
     1081 actgttgagg cgttaagaaa tggcttaatc ggctaccagt caggagatgt tgtacctttt
     1141 cgtgcaattg cttatctatt agacggtttg gattgagcaa gagtttctta aaggtatcac
     1201 ggtaatcgtc attgaagaag ctgatttcag gtaaatgctc tgcattgtac tggtgagcca
     1261 gcttttcaaa ttcgaggcct gttgccattt tccatccttc accatagagg tagacatttg
     1321 ggtgaatggc agataattca gtttgaatct ggttgattgt ttcgctatct aaaataccca
     1381 taaggtcgaa acggaagcca tcaaaaccat agagttctag ccattggcga agggattgtt
     1441 tgatgtagtt gcggaccata ctgcgctcgc tggcaacgtc gttaccgcag aatgtcccgt
     1501 ctgttcgcat gccgtggtca tcatagcgat agaagtagcc tggaacgatg agctcaaatg
     1561 cgtacttttc agcatgatag acgtggttgt aaacaacgtc cataataact gaaatatctg
     1621 catcatggta ggcctggata gcttcttgta gctctagaat acgtgcgtaa ggatcgtttg
     1681 gttggctaga ataggaacct tctggcagat tgtattgcat agggtcgtag ccccagttgt
     1741 aaacagcttg gggtttattt tcatccacac tgccaaaatc ataaacaggc aataattgaa
     1801 tatgggtaac gcctagttct ctgatgtaat ccattcctag cttcattcca tccatggtag
     1861 gagactctgt caagccgaga aattgactgc gatgcttgaa accagcttct ttttgccatg
     1921 agaaatcacg gacgctcatt tcatagataa tggcctgaga aataggtcgt tgggtcttag
     1981 cacgacgaat tttatgaagt ttatctggat tgataacgta gctgtcacct gagttggctg
     2041 tggaagacag ggcgtaggga tcgtgaacgg aaatccattc accgtttact ttgtggagat
     2101 agtgatagct gtgcgcttct aggtctcctt caacagtaat ttcccaaact ccttttgctc
     2161 ccttcgtcat tgcatgtggg tttccttcta gaacaagaaa aacttgctta gaaataggtg
     2221 cccataactt aaaggttgtt gcctctggtc gataggttgc tcccaaatct tggccattgt
     2281 atgtataact ttgttcaaaa agtgtagagc gaacgatatg accatagccg agttctgttt
     2341 tattacggtc ttggtcgtag atggtatagt ccttgtccaa tgtcagtgca tggaggctgg
     2401 ttagataata gatgacgaga ttatctactt ctagacagga atggataaaa agttgagatg
     2461 tagcatcatt tgattcaagt gagaagctca tatgttcgga gtcgaatcgt tttttcatga
     2521 ccaagcgaat agttgctaca tcgtcaagag atgcttgaaa ttgtcgtaat gccatgagcc
     2581 aaaaggctcc tttctaagtt agagttcggt aatggtatgt ggaatgacac gtcggtagca
     2641 gacttgcttg tcttccttgt tgtcattgat gatttggtgc agagtgttga atgcgacgcg
     2701 tcctagttta atagcgtgga cgtcaacgta ggcttcaata tctagttttg gcttgacgga
     2761 gtcaaaagaa atgataggaa gtttgactcc gacttcgttg agatatttga caacaccttc
     2821 ggcaacggaa gtgtcagttg tgacgattgc atccaggtct tgtttgagta atttcttaga
     2881 aattttgtag gcactgtctt cgagaaggaa gccagacaca aacttgacct tgttttcatc
     2941 caggggaata ttgtgcgatt taagggcgtt cttgtaacct gtataacggt cttgagatac
     3001 gactaactct ttgttaccag cgacgaaggc aatgtttttg taacctttat tgatgaagta
     3061 gttggtagct tcaaagccgg cctggatatt atcattatca actagtgata tgaatggaga
     3121 atccgcctta ccaagaatga ggaaggggaa tttgtgttgg atagctaatt gtaccaatgg
     3181 atcgtcgggc ttggaataga ggaagatgag gccgtccact cgtttgccgt agaccatctg
     3241 agaaatcgct tctagacgtt gttcttcatt ctttcctgtg gagatttgga tggcataatt
     3301 atgatctgcc gcaacttgag aaattcctcg taaggcagta gggaagaagg ggttttgata
     3361 gaaaacatcc gagtcatctg gcaaaaccag tccgataacc tgtgtatagc tagaaactag
     3421 gcttcgagcg ttgaggtttg gatggtagtt gagatccgcc atggctttcc taaccaagtc
     3481 tttcgttttt tggctaatgg cagagctatt ttgaatgaca cgagtaactg tagatggcga
     3541 gacaccagct aattttgcca catctttgat ggtagcacgc atatagacct cctagatttc
     3601 atctctgaat tttgttttga aaaataccca taacaagcat agtacaaata ggatattttg
     3661 tatggttaga agggtagtca caggttgacc aaatagtcca attagacagg ctataagagt
     3721 aggcaggccc agacagttga gagcaaaatt atagcactct gcaaacgttt caaaatggaa
     3781 gaaacgagac tttttagtca aaaagaggaa gaagctggct cctagtagga tgaagagtag
     3841 gtttgttgtt aaaataaagg tcgaaataag agtgatggac agtccgacag ctgctcggtt
     3901 ggcttgatac cattttttag agatggttgc agtcaaggtc tccttactag caaaatcgtc
     3961 agtattcaaa ccttcataag tcaattcgct tagaatatca cttcctttac gaatagtcag
     4021 agactgattg gcgaattgat aagtaaagtc agaactagag ctaacggtat caccaaatgt
     4081 cacttgctcc cagttagtat ttccagtata ggttaggtgg ttgttttcga tgactgcatg
     4141 ttgacggagg tcatctacga cttgatctgt cagaggtgca tagatacctt ctacgaaatt
     4201 ttctaaagga taggtttcga gattgacagt ctgtagggag ctcggaatga gcaagagcgc
     4261 cactaaaaaa agcgttgtaa acaaccgttg ccaaaggttc attagtctac gattggcaaa
     4321 catatttctt ggagagaaga tactagaaaa ataggaaaaa ggatatggga gcattcgttg
     4381 gacctttcat aaatatcaat gtttccccta aaaagggaaa aggtgggaca gggttatccc
     4441 ttgtcgccac cgcttgttaa accagataca aagttctttt gtaggaagaa gaataggata
     4501 ctgataggga gggcaataag aatggcacct gccgcaaagt aggcaatctt catattcttc
     4561 acatcactga tgaaggtttg aagtccgaca gctacagtat agaattcttt ttcacgaagc
     4621 aagaatttgg ataggatgta atctccaaaa ggtcccatga aggcccagag cgcttgtaca
     4681 gcaatcattg gacgaacgag tgggaggacg atttgccaga aacgtctgaa gtgcccggca
     4741 ccgtctagtt ttgcagattc atcaagtgag attggtaccg tatcgaagta gcctttcatg
     4801 agccaagcgt tcattgggat accaccaccg acataaagga agatgaggaa ccagctttgg
     4861 ttgagcgcat tgagcatgag ggccataacg aagaaggcgg taagagcagc catggttggt
     4921 accatttgga taatcaagaa gaagaccaaa ctctgcttac gagcgatgaa gttatagcga
     4981 ctataagcgt aacccgctag taccactata cttgtttgaa taaccattgt caagagagca
     5041 atgataagcg tattgaagta ccaggttccg tatagagtct cgctaaagag cttgctaaaa
     5101 ttgtctagtg taaagttgat attgctatcc aatttgaagg caaccacgtt accagacttg
     5161 aaggcggaca tgattgtaat caagagagga tagatgatga taacagacag aacaatcaga
     5221 tagagatagg ttaaggtctg attgagtatg cgtctagttt ttacagatac tttcatctta
     5281 tacatcctcc atttcaaaag catttagttt tttgaaggca atcatggaaa tactgatgac
     5341 aatcattgag ataatcaatg taactgctgc cgccattgag tattgaggtg atgtacctgt
     5401 ggttaattta taaatccatg aaatcaagat gtcagttgaa cccgctccac caccgacgct
     5461 accaggacca ccattgttga agaggtagat gatagagaag ttgttgaagt tgaaggtgta
     5521 ttgactgatt agtgttggag ctgctactgc taaaatcatc ggaagggtga tgttacggaa
     5581 tttttgaatg gcacttgcac catcaatgta agcagcttca tacaagtcgt ttggaatgga
     5641 ttgcaaaata cccagggtca aaacatagat ataagggaaa ccaagccaag cctgcatcat
     5701 aatgagggca actttagtcc agaatgggtc ggtcttccaa gggataagga aattatcgat
     5761 gaatggtagg aatttgctca ggaatggcaa tacctgtgtg ttaatagcac caacactatc
     5821 gttgaacatg ttactaaaag tcaagatagt tacgaaggca ggaacagccc atgggagcaa
     5881 gaagataaca ccgaagatgc gttttccttt gatgaatggc tgatttgcga tgatggctgt
     5941 caagataccg ataacaattt gagctgttga agcgcagagt gcccagataa ttgtccagcc
     6001 aagtacagaa ccaaatgctg tacggaatgt gcttagtttc cagatatttg taaagttggt
     6061 aataccaatc caatccaaca atgcgcttgg tggcaggtgt ttgaagtcgt agtttgtaaa
     6121 ggcgataaac aatgtaacga caactgggaa gatgatagca aaggtcatcg caatatatga
     6181 tgggataatc aagagatatg ggaaaccatt ctcatagata ccttttacca tttcttttaa
     6241 tgtggtagcc actggcatcc ttgaattcca tcgtttcgcg gtatcacgtg cgtcttttag
     6301 gttgagtgca tagaaagcga gatagacaac tgtgataatt aagtggaaag aaccacgaat
     6361 cagcatgaat agcgaattat cttgtccacg aacactaccg agggtaatca agttgcctaa
     6421 ttcaccagct gcaatagcaa taaaataaac aaggaaggca agcgtaacac ctaaaaagat
     6481 gtatcctttg gctttttgtt tattgtagat ttgtccgaga ccagggatca gggacaaaag
     6541 gagtgcctta cctggatttt gtttcatagt catagtctcc ttatatagct tctgaataga
     6601 cggtggatgt tatcgtcatt tagtttttct tttattactt cgttaactcg ccttgcctaa
     6661 cttcagttat gcctgcggct cattgcctcg tactaaaagt aaactaaaag actatatcta
     6721 ttcagtttat cgttaagtaa taaagtagac tgggaagaga actcccagtc caactttaaa
     6781 cttgactagt tgctgtgttt ttgagcaatt tcgtctgcaa tagctttcac agcgtctgtt
     6841 gcagcagtct tagcgtcttt tgaaccactt gcagcttcga agagcatgtt accagctggt
     6901 gtccaaactg aacccatttc tgagatgttt ggcattggtt gagctgaagc aaactggttg
     6961 ataactgcag ttgtcaattc gtcttttttg cttacagcat attcacgagc ttcagtgtta
     7021 gctggaactt cgtttgtagc gtcgtaaagt gctttttgtt ggtctgtagc agtcaagaag
     7081 tcaacaaact tttgagccat ttcttggttt ttagcgcctg ctggaacaac ccatgcttta
     7141 ccaccaccga atgctgagta gtttttgccg tttacaagag ttggtattgt tgctacacca
     7201 tagtttacac cagcttcttt atatgaagct gctttccaag gaccttcgat gatagcagct
     7261 gctttaccgt ctgtaaattg agtgttaatc aagttgtttg cagcagtacc gtcttgaaga
     7321 ccttgaggcc atttttcgta ccaagtttta gcgtattcaa ttgctttgat agcaccttca
     7381 ttagcaagtc cgatgtcttt tggatttgta ccattttcac cgaatacata gccgccgtaa
     7441 ccagaaagaa gtccgtatgt atagtagaaa ttcgtccagt cagctaggaa tgctgaagtt
     7501 ttaccctctt caccagcaaa tgcgtatttg ctgtctttag caagtgtttc aagttctgca
     7561 aatgtcttag gagcttcagt caacaagtct ttgttgtagt aaagaacaag tgtttcgata
     7621 accgcaggtg aaccgtaaac tttaccaccg tttgttacaa gagcagttgt tgtgtcatct
     7681 gctttgctat catctgcaag agtcaattca gatagttgac cttcagaacc aaggctacct
     7741 acacggtcgt atggtgccat cataacgtct ggggctgaac cagattggtt atccaacgat
     7801 aagttatcca aaccagttaa agcatcacca gttttaacgg taacagatac accattttct
     7861 ttttcgaagc cagctttgac atcgttgatg taagattcat aaccttggtc aacgtagaca
     7921 gtcaattctt tgctaccttc agcagactta gatgcctctg ttgatgaacc tgagtttgag
     7981 catgcagcta aaacagcagt tgatgcagca agaagagcaa cgctcttaag gagattgtgt
     8041 ttcattttct tccctccgaa gataaatagt tttgtgtgac gcttgctttg cgcaaacgtt
     8101 ttcctaagtt gacttcatta tacactagat tgaatccgtt tgcaagcgtt tttataaaaa
     8161 tttttaaaaa tttttttgag tttaaaaaaa tataagaatt tttttgcata tattaatgaa
     8221 aagaaatatg gaatggcatg tgaactaaca gaattaaatg gaaatttctt gatttgttac
     8281 cgttttcagt atataataaa aaagagaaaa cgtttgcgtt gtgcaagcgg attagatagg
     8341 agaacttatg aaagtaagac agagtggtgt gctgatgcac atttcatcat tacctgggaa
     8401 atatggaatt ggttccatgg gccaatcggc ttatgaattt gttgattttt tggtaagaac
     8461 taagcaacgc tattggcaaa tcttaccttt gggaacaacc agttatggag attctcctta
     8521 ccagtcattt tcagcttttg cagggaatac tcattttatt gattttgata tattgattga
     8581 taaaggctgg ctggcagcag aggatttaca cggtgttgac tttgggcaga atcctacaga
     8641 ggtagattac gcagccattt tttatgctcg acgtccatta ctggagaagg tagttgccaa
     8701 catgaaggca caaggcttgc cagaagatta ttggatgttc ttaggggaga atgacttttg
     8761 gattcatacc tttgcggagt atatggctat aaaagagcat tttgatctca aaccttggac
     8821 agagtgggaa gatcaagggg cgcgcttacg ttaccacgat accttggaat attatcgtca
     8881 cttgttggca gatcgaattg attatcaccg cattacacaa tatttattct tttcacaatg
     8941 gaaggcttta aaggcttatg ccaatgccaa aggtgttgag tttgttgggg atatgccgat
     9001 ttatatcgcg gctgattctg ccgatatgtg ggctaatcca catttcttta aaacggatga
     9061 agaaggcaag ccgagtgtgg ttgcaggatg cccaccagat gccttttcgg aaattggtca
     9121 gctttggggc aatccgattt atgattggga agcgatgaag catgacggat ttacttggtg
     9181 ggttgctcgt ttacgtgaat cctttaagat atacgatatg gtacgtatcg accactttat
     9241 cggctttgcc tccttctggg aaattccagc gggtgaagaa acagctgtaa atggctatcg
     9301 tgtcgaagca ccaggttttg agttatttga tacaatcaaa cgtgaactcg gagacttaaa
     9361 tatcatagct gaggatttgg gctctgtgac agacaaggtc atccaattac gtgatacgac
     9421 aggttttcct ggtatgaaag tcctgcagtt tgcttttgat ccagaaggag acagcattga
     9481 aatgccacat aaccatccta ataatgtggt ggcatatacg ggaacccatg acaatgatac
     9541 gattcttggc tggtacgaaa atgaaacaac gaaagaatcg cgtgcattct tagataagta
     9601 cagcaatcgt cgtgaggggg aaacggtcag tcatgctctt ttccgtctgc tctttggttc
     9661 acctgccttt atggcggtgg ctactatgca ggacctactt ggtttggatg gttcagctcg
     9721 catgaacttg ccaaatacgc ttggtggtaa ctggacttgg agaatgacgg cagatcaatt
     9781 gacaccagag attgaagcgg aattgctaga ccttacagaa acttatcgtc gggttaatgt
     9841 gcatttggtt gatgaaaagt cataataaaa tgaactcctc taagcagcta acttagagga
     9901 gtctttgtta tctctgttgt aggacataga gccagtagcg aatccagtag tagatggtgt
     9961 agagtaggca ggctacgata gtccaaatga aaaatgtatt cagcacttta gcaagacttg
    10021 gcaataacca gttaatcagt agagtgacta caaaaataat cactccgata taggtgggca
    10081 atttcttcat gagttttcct cctttgaact tagtataaca aaattatatt tgcagtca
//
LOCUS       DBNFCK010000064         9876 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.064, whole genome shotgun sequence.
ACCESSION   DBNFCK010000064 DBNFCK010000000
VERSION     DBNFCK010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9876
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.064"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..1104
                     /gene="dnaN"
                     /locus_tag="AC1PIW_001272"
     CDS             <1..1104
                     /gene="dnaN"
                     /locus_tag="AC1PIW_001272"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIP6606207.1"
                     /translation="LQALSITKRAISTKNAIPILSTVKITVTSEGITLTGSNGQISIE
                     HFISIQDENAGLLISSPGSILLEAGFFINVVSSMPDLVLDFNEIEQKQIVLTSGKSEI
                     TLKGKEAEQYPRLQEVPTSKPLVLETKVLKQTINETAFAASTQESRPILTGVHFVLTE
                     NKNLKTVATDSHRMSQRKLVLDTSGDDFNVVIPSRSLREFTAVFTDDIETVEVFFSNN
                     QILFRSEHISFYTRLLEGTYPDTDRLIPTEFKTTAIFDTANLRHSMERARLLSNATQN
                     GTVKLEIANNVVSAHVNSPEVGRVNEELDTVEVSGEDLVISFNPTYLIEALKATTSEQ
                     VKISFISSVRPFTLIPNNEGEDFIQLVTPVRTN"
     gene            1196..2077
                     /locus_tag="AC1PIW_001273"
     CDS             1196..2077
                     /locus_tag="AC1PIW_001273"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIP6606208.1"
                     /translation="MTLYILANPNAGSHTAEHIIFKIKESYPQLAVNIFMTVGPEDEK
                     SQIEAILKEFVSSEDQLMILGGDGTLSKALRFWPASLPFAYYPTGSGNDFAKAMNITS
                     LYRSVDAILERKTSRIYVLNSSYGTVVNSMDFGFAAQVINGSTNSILKKILNKVKLGK
                     LTYLFFGIKTLFSKQAINLELTLDEKSYQLDNLFFISVANSLYFGGGIMIWPTASAKK
                     KEVDIVYFKNGNFYQRLQSLLALLTKRHESSHTIQHLTGVDVVLKSKEKLLLQIDGET
                     CTANEVTLTYQERSMYL"
     gene            2087..2290
                     /locus_tag="AC1PIW_001274"
     CDS             2087..2290
                     /locus_tag="AC1PIW_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HIP6606209.1"
                     /translation="MYQLGTFVEMKKPHACVIKSTGKKANKWEVIRLGADIKIRCTNC
                     DHVVMMSRHDFERKMKQVLPSEA"
     gene            2394..2753
                     /locus_tag="AC1PIW_001275"
     CDS             2394..2753
                     /locus_tag="AC1PIW_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIP6606210.1"
                     /translation="MKQLAQQIRVLRTAKNLSQDELAEKLYISRQAVSKWENGEATPD
                     IDKLVQLAEIFGVSLDYLVLGKEPEKEIVVEQRGKMNGWEFLNEESKRPLTRGDVVLL
                     IFLAVMLLGGLFIKHYF"
     gene            2839..3954
                     /gene="ychF"
                     /locus_tag="AC1PIW_001276"
     CDS             2839..3954
                     /gene="ychF"
                     /locus_tag="AC1PIW_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIP6606211.1"
                     /translation="MSLTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTELIIPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPIADIDTINLELILADLESINKRYARVEKMARTQKD
                     KDSVAEFAVLEKIKPVLEDGKSARTVEFTDEEQKIVKQLFLLTTKPVLYVANVDEDKV
                     ADPEAISYVQQIRDFAATENAEVVVISARAEEEISELDDEDKGEFLEALGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQCAGIIHSDFEKGFIRAVTMSYDD
                     LMTYGSEKAVKEAGRLREEGKEYVVQDGDIMEFRFNV"
     gene            4112..4681
                     /gene="pth"
                     /locus_tag="AC1PIW_001277"
     CDS             4112..4681
                     /gene="pth"
                     /locus_tag="AC1PIW_001277"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIP6606212.1"
                     /translation="MTRLIIGLGNPGDRYFETKHNVGFMLLDKIAKRENVTFNHDKIF
                     QADIATTFIDGEKIYLVKPTTFMNESGKAVHALMTYYGLDATDILVAYDDLDMAVGKI
                     RFRQKGSAGGHNGIKSIVKHIGTQEFDRIKIGIGRPKGKMSVVNHVLSGFDIEDRIEI
                     DLALDKLDKAVNVYLEEDDFDTVMRKFNG"
     gene            4681..8175
                     /gene="mfd"
                     /locus_tag="AC1PIW_001278"
     CDS             4681..8175
                     /gene="mfd"
                     /locus_tag="AC1PIW_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIP6606213.1"
                     /translation="MNILDLLHKNKQINQWQSGLNQSTRQLLLGLSGTSKSLIMATAY
                     DCLAEKIMIVTATQNDAEKLVADLTAIIGSENVYNFFTDDSPIAEFVFASKERTQSRI
                     DSLNFLTDSTSSGILVASIVACRVLLPSPETYKGSKIQLEVGQEIEVDKLVKNLVNIG
                     YKKVSRVLTQGEFSQRGDILDIFDMQSETPYRIEFFGDEIDGIRIFDVDSQKSLENLD
                     EISISPASDIILSSEDYSRASQYIQTAIEQSTLEEQQSYLREVLADMQTEYRHPDLRK
                     FLSCIYEQSWTLLDYLPKSSPLFLDDFHKIADKQAQFEKEIADLLTDDLQKGKTVSSL
                     KYFASTYAELRKYKPATFFSSFQKGLGNVKFDALYQFTQHPMQEFFHQIPLLKDELTR
                     YAKSNNTVVIQASSDVSLQTLQKNLQEYDIHLPVHAADKLVEGQQQVTIGQLASGFHL
                     MDEKLVFITEKEIFNKKMKRKTRRTNISNAERIKDYSELAVGDYVVHHVHGIGQYLGI
                     ETIEISGIHRDYLTVQYQNSDRISIPVEQIDLLSKYLASDGKAPKVNKLNDGRFQRTK
                     QKVQKQVEDIADDLIKLYAERSQLKGFAFSPDDENQVEFDNYFTHVETDDQLRSIDEI
                     KKDMEKDSPMDRLLVGDVGFGKTEVAMRAAFKAVNDGKQVAILVPTTVLAQQHYANFQ
                     ERFAEFPVNVDVMSRFKTKAEQEKTLEKLKKGQVDILIGTHRLLSKDVVFADLGLLVI
                     DEEQRFGVKHKERLKELKKKIDVLTLTATPIPRTLQMSMLGIRDLSVIETPPTNRYPV
                     QTYVMETNPSVIRDAMLREIDRGGQVYYLYNKVDTIEQKVSELKELVPEATIGYVHGQ
                     MSEIQLENTLYAFVEGEYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSSRIAYAYLMYRPDKSLTEVAEKRLEAIKGFTELGSGFKIAMQDLSIRGAGNILG
                     AAQSGFIDSVGYEMYSQLLEQAILEKQGKATQRQKSNSEVNLQIDAYLPSDYIGDQRQ
                     KIEIYKRIKNIDSRVNYQELQEELIDRFGEYPDVVAYLLEIGLLKSFLDQVFCHTVLR
                     RQHQVTVTFEPMAGQIFLTQDYFEALSVTNLKAQITENKGKLAVVFNIQQKKEYEILE
                     ELISFAEKLKEIKARKAE"
     gene            8225..8497
                     /locus_tag="AC1PIW_001279"
     CDS             8225..8497
                     /locus_tag="AC1PIW_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HIP6606214.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIQFGNKLLTVKVLEMKDSTKKEDALKMYEIISEKRIEKDEEI"
     gene            8484..8852
                     /locus_tag="AC1PIW_001280"
     CDS             8484..8852
                     /locus_tag="AC1PIW_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsB family cell division protein"
                     /protein_id="HIP6606215.1"
                     /translation="MKKSKILQLNNAFIQSERKKTQHQLAERQQKNRFMGAILILVIF
                     LFMLPAYNLVGTYTNIQQQEKKLAELEKNYEELTKEQKQEAEMVAKLKNEEYAAKYVR
                     AKYQYSKEGEFVYNIPGLPK"
     gene            8849..8962
                     /locus_tag="AC1PIW_001281"
     CDS             8849..8962
                     /locus_tag="AC1PIW_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0009 family protein"
                     /protein_id="HIP6606216.1"
                     /translation="MTESIFKIVEQFLQHSDEKLEELNQKNRELKLEENES"
     gene            8975..>9876
                     /locus_tag="AC1PIW_001282"
     CDS             8975..>9876
                     /locus_tag="AC1PIW_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIP6606217.1"
                     /translation="MQKKLLVWLLPVLFGWQVVDSTEIPFELTAQEEYELTHTIYDQY
                     FQTIPQNPNVFQTENLYSDEELTIAGGQLQPNQHFSITDVLVNSKKELVFQIDDKGYI
                     LASRHLLFDDVIVTETTVEQTYWTKKGFTLLTSPIANEATEIKNDLQPYQAVMVSKIV
                     TTSLGDFAYVTDKGWIAVNNLSKTDNRVEAVQELLTNKYSKDTIGIYVKQLSTGQTAG
                     VNQEKLFYSASIAKLPILYYVQEQLNAGYIDLTTKVKYTAESISFPGAYVAGGSGSLS
                     KTPDNKDYSVEELINKTAKESDNVA"
BASE COUNT         3319 a         1692 c         2001 g         2864 t
ORIGIN      
        1 ctacaagcac ttagtattac taaacgggca atcagtacaa aaaatgctat tccaattctt
       61 tcaacagtaa aaattacagt aactagtgaa ggaatcactt taactggttc aaatggacaa
      121 atctcgatag aacattttat ttctattcaa gatgaaaatg cagggctttt gatcagttct
      181 ccaggttcca ttctcttaga agctggtttc tttattaatg tcgtatccag tatgccggat
      241 ttggtccttg acttcaatga aattgaacaa aagcaaatcg ttttgacaag tggtaagtct
      301 gaaatcacat taaagggaaa agaagcagaa cagtatcctc gtttacagga agttccaact
      361 tcaaaaccat tggtgttaga aaccaaagta ttaaaacaaa caattaatga aacagcattt
      421 gcagcttcta cacaagaaag tcgtcctatt cttacgggtg ttcattttgt tttaacagaa
      481 aataaaaatc taaaaactgt tgcaacagat tcacaccgta tgagccaacg gaaattggtc
      541 cttgatacct ctggtgatga ttttaatgtt gtcattccaa gtcgttctct ccgtgaattt
      601 actgcagttt ttacagatga tattgaaaca gtagaagtct tcttttcaaa taatcaaatc
      661 ctttttagaa gcgagcatat tagcttctat acacgcttat tagaaggtac ctaccctgat
      721 accgaccgct taattccaac tgagtttaaa acaactgcaa tttttgatac tgcaaatctt
      781 cgtcactcga tggagcgtgc tcgtcttctt tcaaatgcaa cccaaaatgg tacagtaaaa
      841 ctagaaattg ctaataatgt tgtatcggct catgtaaatt ctccagaagt tggacgtgtg
      901 aatgaggaat tagatactgt agaagtatca ggtgaagatt tagtaatcag ctttaaccca
      961 acttacttga tagaagcatt gaaagccaca actagtgaac aagtgaaaat tagctttatc
     1021 tcttctgtcc gtccatttac attgattcca aataatgaag gggaagattt tattcaattg
     1081 gttacaccag ttcgtaccaa ctaaataata ttaagaacgg ctaaactagc cgtttttatg
     1141 ttatactaaa aaatagcacc tagcttattt ttatatattt agtgatgggg aataaatgac
     1201 gttatatata ttagctaatc ctaatgctgg tagccatact gctgaacata tcatattcaa
     1261 aataaaagaa agttatccac agcttgcagt taacattttt atgacagttg gtcctgagga
     1321 tgaaaaaagt caaatagagg ctattttaaa ggagtttgtc agtagtgaag atcaattaat
     1381 gattttaggc ggagacggca cactatctaa agctttgcgt ttttggccag ctagtctacc
     1441 gtttgcttat tatccaacag gatctggaaa tgattttgct aaggcaatga atataacatc
     1501 gctatataga agtgtagatg ccattttaga gagaaaaaca agtcggatat atgttttaaa
     1561 cagttcatac ggaacggttg taaacagtat ggattttggc tttgcagctc aagttatcaa
     1621 tggttcaacg aattcaattt tgaaaaaaat tctgaacaag gtaaaacttg ggaagttaac
     1681 ttatctattc tttggtatta aaacattatt ttcaaaacaa gctataaact tagaattaac
     1741 tcttgatgaa aaatcttatc agttagataa tctctttttt atttctgtag caaatagtct
     1801 ttattttggt ggaggaatca tgatatggcc aacagcaagt gctaaaaaga aggaagtaga
     1861 tattgtttac ttcaaaaatg gaaatttcta ccaacgtcta caatcattgt tagccttatt
     1921 aacgaagagg catgaatctt ctcatacgat tcagcattta acaggggtag atgtagtttt
     1981 aaaatcaaaa gaaaaattat tattgcaaat agatggagag acatgcactg caaatgaggt
     2041 aacgttaacc tatcaggaaa gaagtatgta tctttaagga ggaagtatgt accaattagg
     2101 aacctttgtc gaaatgaaaa agccccatgc ctgtgtcatc aaatcgaccg gaaagaaggc
     2161 taataaatgg gaggttatcc gtctaggagc ggatattaaa atccgctgta ccaactgtga
     2221 ccatgtcgtt atgatgagcc ggcatgattt tgaacgaaaa atgaaacaag ttctgccaag
     2281 tgaagcctag ttgacagttt gcaactactg gttgcgaatt tttcctaaaa agtgctagtc
     2341 aactgttcct ttctaaacta taatagactt agaaaaaaga ggaggaaatg atcatgaagc
     2401 agttagcaca gcaaattcga gttttacgca cagccaagaa cctatcccaa gatgagttgg
     2461 cagagaaact ctatatttcc cgtcaggctg tttccaagtg ggaaaatggt gaagccacac
     2521 cagatattga caaactggtc cagctggcag aaatctttgg tgtcagtctg gattatctgg
     2581 ttttagggaa agaacctgag aaggaaattg tggtggaaca acgaggaaaa atgaatggtt
     2641 gggaattctt gaacgaagaa tccaaacgac ctcttacaag aggagatgtt gtccttctca
     2701 tttttcttgc agttatgctt ttaggtggat tatttattaa gcattatttt taacgaagct
     2761 tgccaaacag caagcttttt ctctactttt ctgctataat agtcatgatt gaatttttaa
     2821 ttggagagta aaaaaaacat gtctttaaca gcaggaatcg tcggtttgcc aaacgttggt
     2881 aaatcaaccc tatttaacgc aattaccaag gcaggagcag aagctgcaaa ctaccctttc
     2941 gcaactattg atccaaacgt cggcatggta gaagtgcccg atgagcgtct gcagaagttg
     3001 accgaactca tcatccctaa aaagacagtg ccaacgactt ttgaatttac tgatattgct
     3061 ggtatcgtaa aaggtgcttc taaaggtgaa ggacttggaa ataaattctt ggccaatatc
     3121 cgcgaagttg atgcgattgt ccacgttgtg cgtgcctttg acgatgaaaa tgtcatgcgt
     3181 gaacaaggcc gtgaagatgc ctttgtggat ccaatcgctg atattgatac cattaaccta
     3241 gaattgattt tggcggacct agagagcatc aacaagcgtt atgcgcgtgt agaaaaaatg
     3301 gcccgtacgc aaaaagacaa agattctgtc gcagaatttg cagttcttga aaaaatcaaa
     3361 cctgtcctag aagacggaaa atctgcccgc acagttgagt tcacagatga agaacaaaaa
     3421 atcgttaagc aactcttcct cttgaccact aaacccgtcc tctatgttgc caatgttgat
     3481 gaagacaagg tagcagatcc tgaggctatc agttatgttc agcaaatccg tgactttgca
     3541 gcaacagaaa atgcagaagt ggtggtgatc tctgcgcgtg ctgaagaaga aatttcagaa
     3601 ctcgacgatg aagacaaggg cgagttttta gaagcactcg gattaacaga atctggcgtg
     3661 gacaaattaa cacgtgcagc ctatcacttg cttggtctag gcacctactt tacagcaggg
     3721 gagaaggagg ttcgtgcttg gaccttcaaa cgtggtatga aagctcctca gtgtgcaggc
     3781 attatccatt cagacttcga aaaaggtttt atccgtgccg ttaccatgtc atacgatgat
     3841 ttaatgacct acggctctga gaaggctgtt aaagaagcag gacgccttcg tgaagaagga
     3901 aaagagtacg tggttcaaga tggggacatc atggagttcc gctttaacgt ttgattgcta
     3961 aactgtcctt attcttcgtt gttttacctt gccataccat cagtattgtc tgcggtttca
     4021 acgcctagac taagaacagt ttatcgcatc aaagaggatg attacttggt tggaaagctt
     4081 tttccagcct tttggtattt aaggagaaaa aatgacacga ttgattatcg gtttgggaaa
     4141 tcccggagac cgctattttg aaacaaaaca taacgttggt tttatgctgc tggataagat
     4201 tgctaagcgt gaaaatgtga cctttaacca cgataagatt ttccaagcag acattgccac
     4261 aacctttatt gatggtgaaa aaatttacct ggtcaaacca acgactttca tgaatgaatc
     4321 aggtaaggct gtccatgcct taatgactta ttatggtctg gatgcaactg atattttagt
     4381 agcttatgac gacttggaca tggctgttgg aaaaatccgt ttccgtcaaa aaggctcagc
     4441 tggtggccac aatggtatca aatccattgt caagcatatt ggaacacagg aatttgaccg
     4501 tatcaagatt ggtattgggc gtcctaaagg aaaaatgagc gttgtaaacc atgtcttgtc
     4561 tggctttgat atagaggacc gtattgaaat cgacttagca ctagataaac ttgacaaggc
     4621 tgtcaacgta tatctcgaag aagatgattt tgatacagta atgagaaagt ttaacggata
     4681 atgaatatac tcgatttact tcacaagaac aagcaaatca atcaatggca gtcaggattg
     4741 aatcaatcaa cacgtcagtt attattagga ctatctggga ctagtaagtc acttataatg
     4801 gcgactgcct atgattgttt ggccgaaaag attatgattg tgactgctac tcaaaatgat
     4861 gctgagaaat tagttgcaga tttaacagcc attattggaa gtgagaatgt ttacaacttt
     4921 tttacagatg atagtcctat tgccgagttt gtttttgcat caaaagaacg gactcaatca
     4981 aggattgaca gtttaaattt cttgacagat tctaccagtt caggaatttt agttgctagt
     5041 atagttgctt gtcgggtctt attgccgagt cctgagactt ataaaggttc taaaatacag
     5101 cttgaagttg gtcaagaaat agaagttgac aaacttgtaa agaaccttgt caacatcggc
     5161 tacaaaaaag tgtctcgtgt attaacccag ggagaattta gccaacgagg tgatattcta
     5221 gatatttttg acatgcaatc agaaacacct taccgaatag aattttttgg agatgagatt
     5281 gatggcattc gtatctttga tgttgacagt caaaaatcct tagaaaatct agacgaaatc
     5341 tcgatctctc cagcttccga tatcattttg tcttcagaag actatagtag agctagccaa
     5401 tacattcaga cagctattga acaatcgact ctagaggagc aacagtctta cttacgagaa
     5461 gtcttagctg atatgcagac ggagtaccgg catccagatt tgcgtaaatt cttgtcctgt
     5521 atctatgaac aatcatggac attgttggat tacttgccaa agagttctcc cttatttttg
     5581 gatgattttc ataaaatcgc tgataagcag gctcaatttg aaaaagaaat tgcagacttg
     5641 ttgacagatg atttacagaa gggtaaaaca gtgtcaagtt tgaaatattt tgcctcaact
     5701 tatgctgaat tgagaaaata caaacctgcc accttctttt caagttttca aaaaggttta
     5761 ggaaatgtaa agtttgatgc tctttatcaa ttcacacagc atcctatgca ggaatttttc
     5821 caccagattc cactgttaaa agatgaattg actcggtatg cgaaatcaaa caataccgtt
     5881 gttattcaag cgagttctga tgtaagttta cagactttac agaaaaattt acaagagtat
     5941 gatatccatc ttccagtaca tgcagcagat aagttagtag aaggtcagca acaggttacg
     6001 attggtcagt tagcttcagg ttttcatttg atggatgaga aactagtttt catcactgaa
     6061 aaagagattt tcaataagaa aatgaagcgt aagacacgta gaaccaatat ttctaacgct
     6121 gaacgtatta aggactatag tgaattagca gttggtgact atgttgttca ccatgttcat
     6181 ggtatcggtc aatacttagg aattgagact attgaaattt caggcattca tcgtgattat
     6241 ttaacggttc aatatcaaaa ctccgatcgc atttctattc ctgtagaaca aattgatctt
     6301 ctctccaaat acttagcgtc cgatgggaaa gctccaaaag ttaataaact aaacgatggc
     6361 cggttccaac gaaccaagca aaaagttcag aagcaagtgg aggatattgc agatgatttg
     6421 attaagcttt atgcggagcg tagtcagctg aaaggctttg ccttttcacc tgatgatgaa
     6481 aatcaagttg aatttgacaa ctactttaca cacgtggaaa ccgatgacca actccgttcc
     6541 attgatgaaa tcaaaaaaga catggaaaaa gactctccaa tggaccgtct gttagtagga
     6601 gatgtcggat ttggtaagac agaggtagcc atgcgcgctg ctttcaaagc agtcaatgat
     6661 ggcaagcaag tagctattct tgtacctacg actgttttag cccagcaaca ctatgctaat
     6721 ttccaagaac ggtttgcaga atttcccgtc aatgtggatg ttatgagtcg ttttaaaaca
     6781 aaggcagagc aggaaaaaac acttgagaag ttgaaaaaag gccaagttga catcttgatt
     6841 ggtacccatc gccttctatc aaaagatgtt gtttttgctg atttaggttt actggttatt
     6901 gacgaagagc aacggtttgg tgtcaagcat aaggaacgct tgaaggaact gaaaaagaaa
     6961 atagatgtcc taaccttgac tgcaactccg attcctcgga cattgcaaat gtccatgctg
     7021 ggaatccgcg acttatcagt aattgagact ccaccgacaa atcgctatcc tgtacaaact
     7081 tatgtgatgg aaacaaatcc ttccgttatc cgagatgcaa tgcttcgaga gatagatcgc
     7141 ggaggtcagg tttactatct ttacaataaa gttgatacca ttgaacagaa agtgtctgaa
     7201 ttaaaagaat tggttccaga agctactata ggatacgtcc atggacaaat gtcggagatc
     7261 caattagaaa atactctcta tgcttttgta gagggtgaat atgatattct agtgaccaca
     7321 acaatcatcg aaacaggtgt tgatattcca aatgctaata cactgtttat tgaaaatgca
     7381 gatcatatgg gactgtcaac tctttatcaa cttagaggac gtgttggccg ttccagtcgg
     7441 attgcctatg cctatctcat gtaccgtcca gacaagtcct tgacagaagt agctgaaaaa
     7501 cgtttggagg caatcaaggg ctttacagaa ctaggatcag gtttcaagat tgccatgcaa
     7561 gatttgtcca ttcgtggtgc gggaaatatt ttaggtgctg ctcagtcagg ttttattgat
     7621 tctgtgggat atgagatgta ttcacaattg ctggaacaag ctatcttgga aaaacaaggt
     7681 aaggcaaccc aacgtcagaa gagtaattcc gaggtcaatt tacagattga tgcctattta
     7741 ccaagtgatt atattggtga tcagcgacaa aaaattgaaa tttacaagcg tataaagaat
     7801 attgacagtc gtgtcaacta tcaagaattg caagaagaat tgattgaccg ttttggagag
     7861 tatcctgatg tagtagccta cttgcttgaa atcggtctac tgaagtcatt tttagaccaa
     7921 gttttctgtc atacagttct tagacgacaa catcaagtga cagtaacatt cgaacctatg
     7981 gctggtcaaa ttttcctaac gcaagattac tttgaagctt tatctgtgac aaatttgaaa
     8041 gctcagataa cggaaaataa aggaaaacta gctgttgtgt ttaacattca acagaaaaaa
     8101 gaatatgaaa tattagagga attgatttct tttgccgaaa aattaaaaga aattaaagca
     8161 aggaaggcag aataaatttc tcaaatacct tgaaaaatgg taaaataatg aactgaggta
     8221 aagtatgaga ctagataaat atttgaaagt atcccgcatt atcaagcgtc gaacagttgc
     8281 gaaggaagtt gccgataaag gacgaataaa agtcaatggg attttggcga aatcttcaac
     8341 agatttaaaa gtaaatgatc aggttgaaat ccaatttggt aataaactac tcactgtaaa
     8401 agtacttgaa atgaaagatt cgactaaaaa agaagacgca ctgaaaatgt atgaaattat
     8461 cagtgaaaaa aggatagaaa aagatgaaga aatctaaaat tctacaactg aataatgcct
     8521 ttattcagtc ggagcgtaaa aaaactcagc atcaattggc agaacgccaa caaaaaaatc
     8581 gttttatggg tgcaattctc attctagtta tctttctttt tatgttgcct gcctataatc
     8641 ttgttgggac ttacactaat attcagcagc aggaaaagaa acttgctgaa ttggaaaaaa
     8701 attacgaaga actgaccaaa gaacagaagc aggaagcaga gatggttgca aaattgaaaa
     8761 atgaggaata cgcagcaaag tatgttcggg ctaagtatca atactctaaa gaaggggaat
     8821 ttgtctacaa tattccaggg ttaccaaaat gaccgaatct atttttaaaa ttgtagagca
     8881 atttttacag cattctgatg aaaaattaga agagttgaat caaaagaata gagaattaaa
     8941 gttagaagaa aatgaatcgt agaaaggagg gagtatgcag aaaaaactgc tcgtatggtt
     9001 attgccagtt ttatttggat ggcaggtggt cgatagtaca gaaataccat ttgaactcac
     9061 agcgcaagaa gaatacgaat tgactcatac tatctatgat cagtattttc aaactatccc
     9121 tcaaaaccca aatgtttttc aaactgagaa tctttattcg gatgaagaat tgactatagc
     9181 aggtggacaa ttacagccga accaacattt ttctattacg gatgttctag tcaatagcaa
     9241 aaaagaattg gttttccaaa tagatgacaa gggttacatt cttgcaagta ggcatctact
     9301 atttgatgat gtaattgtta cagaaactac tgttgagcaa acttattgga caaaaaaagg
     9361 tttcacattg ttaacctcac caattgctaa cgaagctacg gaaataaaaa atgatttaca
     9421 accctatcaa gctgttatgg tttctaaaat tgtgacaact tccctaggtg attttgccta
     9481 tgttacagat aaaggttgga ttgcagtaaa taatctttct aaaacggata atagagtaga
     9541 agccgttcaa gagttgttga ctaacaaata ttccaaagat actattggta tatatgtgaa
     9601 acaattgtca acaggtcaaa ctgcaggtgt aaatcaagag aaactattct attctgccag
     9661 cattgctaaa ctaccaattc tttattacgt tcaagagcaa ctgaatgctg gatatattga
     9721 tttgactact aaagtgaaat atacagccga atctatatcc ttcccaggag cttatgttgc
     9781 tggaggaagt ggttcactgt caaaaactcc tgacaacaaa gattattcag tggaagaatt
     9841 gatcaataag actgctaaag agtcagataa tgtagc
//
LOCUS       DBNFCK010000065         9634 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.065, whole genome shotgun sequence.
ACCESSION   DBNFCK010000065 DBNFCK010000000
VERSION     DBNFCK010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9634
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.065"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(84..422)
                     /locus_tag="AC1PIW_001283"
     CDS             complement(84..422)
                     /locus_tag="AC1PIW_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HIP6606218.1"
                     /translation="MSTNIYDIANELERAIRNLPEYKAVEAVKVSVEGNSEAKEILES
                     YISFQKEIQSKLQAGEIPTEADQKKMLDFNKKVQGNPLLTEYFSKQQQLGTYVADLER
                     IIFKPLNELL"
     gene            complement(432..1535)
                     /locus_tag="AC1PIW_001284"
     CDS             complement(432..1535)
                     /locus_tag="AC1PIW_001284"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HIP6606219.1"
                     /translation="MKKTILIVGLGLIGSSLALCIRKDHPDYHLIGFDPNEQSCQIAL
                     GKGMVDEVSTELEGVAKQADVILLCVPIQVSLRLIEEFSALELKDTVLITDAGSTKSA
                     IVAAAEEHLAPRQINFIGGHPMAGSHKSGASAADLHLFENAYYIMTPCQATKQDAVPR
                     LTDLLSGTGARFVQIDAAEHDRVTSQISHFPHVLASSLMHQAGEYAQDHPFTNNFAAG
                     GFRDMTRIAESEPSMWTSILLTNADSILNRIEEFQARLSEISDVLRSGDQKAIWDFFD
                     KGRQTRKAMEIHKRAGVDSFYDLFLSVPDEEDTILKVLEVLRGISIVNIRINEENRED
                     IHGILQITLKNKGNLEDAAKRLREQTTYKIHLD"
     gene            complement(1543..2709)
                     /gene="aroC"
                     /locus_tag="AC1PIW_001285"
     CDS             complement(1543..2709)
                     /gene="aroC"
                     /locus_tag="AC1PIW_001285"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIP6606220.1"
                     /translation="MRYLTAGESHGPRLTAIIEGVPAGLPLTAEDINEELKRRQGGYG
                     RGSRMQIETDRVEITAGVRHGKTTGAPITLNVTNKDHQKWLDIMAVEDIEEKLKSKRR
                     IKHPRPGHADLVGGMKYRFDDLRNSLERSSARETTMRVAVGAVAKRILAELDIEIANH
                     VVVFGGKEIDVPDGLSVAEIKERAAQSEVSIVNQEREEEIKAYIDQIKRDGDTIGGVI
                     ETVVGGVPAGLGSYVQWDKKLDAKLAQAVVSINAFKGVEFGVGFQAGYLKGSQVMDEI
                     LWSQEKGYTRRTNNLGGFEGGMTNGEALIIRGVMKPIPTLYKPLMSVDIDTHEPYKAT
                     VERSDPTALPAAGVVMESVVATTLATEILEKFSSDNMEELKAAVASHRDYVKNF"
     gene            complement(2713..3387)
                     /gene="aroD"
                     /locus_tag="AC1PIW_001286"
     CDS             complement(2713..3387)
                     /gene="aroD"
                     /locus_tag="AC1PIW_001286"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HIP6606221.1"
                     /translation="MKIVVPIMPRNLEEVEAIDVERLAEADIVEWRADYLLKDDILRV
                     APAIFEKCSGKEVVFTIRTTREGGHLDLDDQEYVNVIKEVATLYQPDYIDFEYYSYKS
                     VFEQMLEFPNLVLSYHNFEETPSNYMEIMSELTSLSPAVVKMAVMAKTEQDVLDVMNY
                     TRGFKSLNTEQIFATIAMGELGKLTRIAGVITGSCWTFASLDETSAPGQMSLSNTRKF
                     LEILEN"
     gene            complement(3384..4547)
                     /locus_tag="AC1PIW_001287"
     CDS             complement(3384..4547)
                     /locus_tag="AC1PIW_001287"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HIP6606222.1"
                     /translation="MKIKVNRIAEQKINRGIQLLDSADFSGVSFDEDRLAVLYSESGS
                     YLGQAYLSKQNKGIGWLFSRSNQDLTMDFFVKLFVDAKQKRQHFYQSEMTNAFRLFNQ
                     DGDSFGGMTVDLYDEYAVFSWYNTFIYSVKEMIVAAFQQVFPEVRGAYEKIRFKGLAF
                     ESAHLYGEEAPEYFTVLENGVHYQVFMNDGLMTGIFLDQHEVRGSLVDGLAAGKSLLN
                     MFSYTAAFSIAAAMGGAEETTSVDLAKRSRELSKAHFKVNSLDLDNHHFVVMDVFEYF
                     KYAKRKGLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNTGGTIIASTNAA
                     NVSVEKFKRQIEKGFDVRKHRYEATYRLPADFVINKNDESSNYLKVFTIQVDK"
     gene            4663..6843
                     /locus_tag="AC1PIW_001288"
     CDS             4663..6843
                     /locus_tag="AC1PIW_001288"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HIP6606223.1"
                     /translation="MKKLIDYVKKSGQTRLGFVLILLIIYWLKTLWAYYVDFNLDTQG
                     FFQNFIAIMNPLPVGILLLGLSLYIKNTKVFYSVAMIIYIILSVWLFSNAVYYREFSD
                     YISISTIMATSSVSAGLGEAALKLFRLWDIVYFLDFIIFGILVYRKLLRWDKKTFHKK
                     SSFAITALSVMLFSINLFLAEIDRPELLTRGFSNTYIVRALGLPAFLGYNANQTYNAH
                     KNRSGASATDLEPVKQYITEHYAQPNDNYFGIAKGRNVIYLHLESLQQFVIDYKLNVN
                     GVEHEVTPFLNSLYHSSSTLAFSNFFNQVKAGKTSDAETMIETSLFGLNQGSFMVQYG
                     GSNTQQAAPYILSQNGGYTSAVFHGNAASFWNRNNTYKQWGYNYFFDQSYFSKATAEN
                     SFQYGLNDKIMFADSIQYLEHLQQPFYTKFITVSNHYPYTTSLIGDEIGFPLANTDDE
                     TINGYFATANYLDASIKAFFDYLKATGLYENSIIVLYGDHYGISNSRNPDLAPLIGKD
                     SQTWSDFDNAMMQRVPYMIVVPGMTNGGIFDTYGGEIDALPTLEHLLGIKSDNYLQVG
                     QDLLSSQHSQIVALRTAGSFITPKYTSYEGKIYYTETGLEITNPDETTQKEIDAIKEA
                     TAAQLATSDAIQTGDLIRFFDNGLPALDPSQYNYIDSLAAEIALENKLGKQSTSLYST
                     NGNKSTIELFKAPSYLELHGSTTATSTPNTGTSTSSTSTSSNSR"
     gene            complement(7039..9543)
                     /locus_tag="AC1PIW_001289"
     CDS             complement(7039..9543)
                     /locus_tag="AC1PIW_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HIP6606224.1"
                     /translation="MKQGKTLLYVAGASLICAVALVTYQASNYREQSNHQKTTSIETS
                     QSKQSKEIPSDSKKEVPGIDKATDDVFLLTDESQIEEKTDLGIIVNHGDHKHFFFYSD
                     LKNTKWAYLIPENYQEKNHSSQPKRSQLSSGSKQIEDEYVFDPKDIVAEDANGYTVRH
                     GDHYHYILKSSLGGTTHRQLTSDSRQSPTLPIVHQQEGIPGIDFQTSDGFLFDGQNIS
                     GVTETGILVRHGKHLHLIHFETLKRSKWSYLVNQYKPNVESDKKAQPEVENTEYQTKL
                     DYLAKELNLEPSRFKKVIVDGQIGLEYPHGDHTHIVLLKEIDTTKPFESPEDRILKQK
                     DGETLEQRKERLIKQYMERFKVKREDITIDGNYMSVRHGDHAHVYKIDPSLPDDPERD
                     VKTETVNLAIEKQLVYGPFYTEGSTENLTRNGVHQKYRPEGLQDIKNFILVTFSTNSD
                     FGNFVVNGKKTKRVYYLVRKDLNWEDLNIAYPQTLQQKGRVFNGWNATLPKSGKMARE
                     HQSFYADFDNVYRKPTKNIYTPSDDVSNIDLSDYVPVKYSAIANGRLKLNGEIRAGFI
                     YFVKSDLTWKQAKEQGLVVPEPVSSKDYEFIEFRTVITGGEKDTDYVSATTSLAAFGT
                     TAPRIGPYIANNTENPTDINDPSRHPNYYWHDPKNYVALAFRAGEGGQLQTHLGTSKT
                     VVYLVRKGLTLNQAGIFPPSLKSDTGYKRDYTKPVIPNVAWDTPILEDTVYDIHFDKV
                     ESDSKDGTVEPGDTWLPGNPLANTPDVPNGEEDADSWLDDLLTPSPEATDIETVTEAE
                     TTTEDTKATESSTAAPIDIPKKSSTEEEPSEDFIFP"
BASE COUNT         2839 a         2160 c         1607 g         3028 t
ORIGIN      
        1 tggagataag aactctacgt aagcagtgta tactaggcgt caatccatga cctaacaccc
       61 tgctcctatc aaatataaat atactacaac aattcgttga gaggcttgaa gatgatacgt
      121 tccaaatcag ctacataagt tcccaactgt tgttgcttgc tgaaatattc cgtcaaaagt
      181 ggatttccct gaactttctt gttgaaatcc aacattttct tttggtcagc ttcagtagga
      241 atttcaccag cctgcaattt agattggatc tctttttgga aagaaatata actttccaat
      301 atttctttgg cttctgaatt cccctcaact gagaccttaa cggcttccac cgccttatat
      361 tcaggcaggt tacggattgc acgttccaat tcatttgcga tatcatagat atttgttgac
      421 atatttttct cctaatctaa atggattttg taagtcgtct gctctcttaa acgtttcgca
      481 gcgtcttcca aatttccctt gtttttaagc gtgatttgta aaataccgtg tatatcttca
      541 cggttctctt cattgatacg aatattgaca atggaaatac cccgtaagac ttccaaaacc
      601 ttcaaaatcg tatcttcctc atcaggaaca ctaaggaata aatcgtagaa agaatcaaca
      661 cctgcacgct tgtgaatttc catggcttta cgagtttgac gtcccttatc aaaaaagtcc
      721 caaattgctt tttgatcgcc agatctgaga acatctgaaa tttctgaaag acgtgcttga
      781 aattcctcga ttcgatttag aatagaatct gcattggtta gcaaaatgct agtccacatg
      841 ctcggctcac tttccgcaat ccgtgtcata tctctaaatc ctccagctgc aaaattattc
      901 gtaaagggat gatcttgagc atactcacca gcttgatgca tcaaactaga agccaatacg
      961 tgtggaaaat gtgaaatttg actggtcaca cggtcatgtt ctgctgcatc aatctgaaca
     1021 aaacgtgccc ccgttccaga taacaagtct gtcaatcggg gaaccgcatc ctgcttggta
     1081 gcctgacacg gtgtcataat gtagtaagca ttctcaaata gatggagatc tgctgcactg
     1141 gcaccagatt tatgactacc tgccataggg tgtccaccga taaaattaat ttgtcttggc
     1201 gccaaatgtt cctcagcagc agccacaatt gcagacttcg ttgaacctgc atctgtaatc
     1261 aagaccgtat ccttcaattc aagggcacta aattcttcaa tcaaacgcaa agaaacttga
     1321 attggaacac aaagaagaat cacatcagcc tgctttgcta caccctccaa ttccgttgat
     1381 acctcatcaa ccatcccctt gcccaaggca atttggcatg attgttcgtt aggatcaaag
     1441 ccaatgaggt gatagtctgg atggtctttg cgaatacaga gcgcaagtga acttccaatc
     1501 aaccctagac ctacaattaa aatcgttttt ttcattaatt acctagaaat ttttgacata
     1561 gtcgcgatga ctggctaccg cagctttcaa ttcctccata ttatcggaag agaacttctc
     1621 taggatttct gtcgctagag ttgtagctac gactgattcc atgactacac cagctgctgg
     1681 gagagccgtt ggatctgaac gttccacagt agctttataa ggctcatgag tatcaatgtc
     1741 aaccgacatc aatggcttat agagggtcgg aattggtttc ataacaccac gaatgattag
     1801 ggcctcgcca ttggtcatac caccttcaaa accacctaga ttattggttc ggcgtgtata
     1861 tcctttttct tgggaccaga gaatttcgtc catgacttgg ctccctttga gataaccagc
     1921 ttggaagcct actccaaatt ctactccctt aaaagcattg attgaaacaa cagcctgcgc
     1981 taattttgca tctaactttt tatcccattg cacataagac cccaagccag ctggaactcc
     2041 tccaacaaca gtctcaatca ctccgccaat agtatctcca tctcgcttga tttgatcaat
     2101 ataggctttg atttcttctt cacgctcttg gttgacaatg gatacttctg actgggctgc
     2161 acgctctttg atttctgcta cgcttaaacc atctgggaca tcaatttcct tccctccgaa
     2221 aacgactaca tggttagcaa tttcaatatc caactcagcc aaaatccgtt tggcaaccgc
     2281 accaacagcc acacgcatag ttgtctcacg cgcactagaa cgctccaagg aattacgcaa
     2341 atcatcaaag cgatatttca taccaccaac taggtcagca tgtcctggtc gtggatgttt
     2401 gatacgacgc ttacttttga gtttctcttc gatgtcctca actgccataa tatccaacca
     2461 tttttgatgg tctttgttgg tcacattcaa tgtaatcggg gcaccggttg tcttaccgtg
     2521 acgaacacca gctgtaatct ctacgcgatc tgtttcaatc tgcatacgac taccacgacc
     2581 gtatccacct tggcgccgtt ttaactcttc attaatatcc tcggcagtta aaggaagacc
     2641 cgctggaact ccctcaataa tggctgttag acgtggtcca tgagactcac cagctgttaa
     2701 ataacgcata acttaattct ccaaaatttc cagaaactta cgagtattgc ttaaagacat
     2761 ttgtcctggt gcagatgttt catctaagct ggcaaaagtc cagcaggagc ctgtaatcac
     2821 acctgcaata cgtgttaact tccctagttc acccatggcg attgtcgcaa atatctgctc
     2881 agtgttcagt gatttaaaac cacgagtata attcataaca tctaggacat cctgctctgt
     2941 cttagccatt acagccattt taacaacagc tggtgatagg ctagtcagtt cagacatgat
     3001 ttccatataa ttagacggtg tttcttcaaa attatgataa ctcaacacaa gattaggaaa
     3061 ctccaacatc tgctcaaaaa ccgacttata agagtagtat tcaaaatcaa tataatctgg
     3121 ctgataaagc gttgctactt ctttgattac attgacatat tcttgatcgt ccaaatccaa
     3181 gtgaccgcct tcacgtgttg tccgaatggt gaaaactact tccttaccac tacatttttc
     3241 aaatatagca ggtgctactc ttaaaatatc atctttcaga agatagtctg ctcgccattc
     3301 aacaatatct gcctctgcta aacgttctac atcaatggcc tctacctctt ccagatttct
     3361 aggcataata ggaactacta ttttcattta tctacctgta tcgtaaagac ctttaggtaa
     3421 ttactacttt catcattttt attgattaca aaatcggctg gcaagcgata agttgcctca
     3481 tagcgatgtt ttctaacatc aaaccctttt tcaatctgcc gcttaaattt ttcaacagaa
     3541 acatttgcgg cgttggtcga agcgataata gttccacctg tatttagtat ttccaaagat
     3601 tgagaaatca acttgtgata atctttggct actgaaaagg tttgtttctt atttcgtgca
     3661 aagcttggcg gatccaagac aatcacatca taagttaagc ctttacgttt ggcatatttg
     3721 aaatattcaa atacatccat tactacaaag tggtgattat ctaagtctaa actattaacc
     3781 ttaaagtgag ctttagaaag ttctcgcgag cgtttggcta agtcaacaga agtcgtttcc
     3841 tctgctccgc ccatggcagc agcaatagaa aatgctgccg tatacgagaa catgttcaaa
     3901 agtgacttgc ctgccgccag gccatccacc aaggaaccac gtacctcatg ctgatccaaa
     3961 aagattcccg tcatcagtcc atcattcata aagacctgat agtgaactcc attttccaac
     4021 acagtgaaat actcaggggc ttcttctcca taaagatgag cagactcaaa ggccaaacct
     4081 ttaaaacgaa tcttctcata agctcctctt acttctggaa atacctgttg aaatgcagca
     4141 acaatcatct ctttgacaga ataaataaag gtattatacc atgaaaaaac tgcgtattca
     4201 tcatacaagt caaccgtcat tccaccaaaa ctatctccat cttgattaaa taaacgaaaa
     4261 gcgttcgtca tctcagattg ataaaaatgt tgacgtttct gtttagcatc cacgaataat
     4321 ttaacaaaaa aatccatggt caagtcttga ttgcttctac taaacagcca gcctatcccc
     4381 ttattctgtt tggacaaata agcctgaccc aaatagcttc cagactctga atacaaaaca
     4441 gccagcctgt cttcatcaaa tgaaacacct gaaaaatcag cgctatccaa gagttgaatc
     4501 cctctgttta ttttctgttc tgcaatacgg tttaccttta ttttcataga attattataa
     4561 catgtttctt catatatgcc aaaaaaactc taaactttga ggcttatttt agaattatgg
     4621 tataatgaac taaactttta gaaaggacca tgaggtttta ttgtgaaaaa actaattgac
     4681 tatgtcaaga agagcggaca gactagactt ggtttcgtct tgattttgct aatcatttac
     4741 tggctcaaaa cactctgggc ctactatgtt gatttcaact tagatacaca agggtttttc
     4801 caaaatttta ttgctattat gaatcctcta ccagtcggga ttttattact cggtctctcg
     4861 ctttatataa agaatacaaa agtcttctat agcgtagcga tgatcattta tattattctt
     4921 tccgtctggc tcttttcaaa tgcagtctat tacagagaat tttccgatta tatttccatc
     4981 tctactataa tggctacctc tagtgtatct gctggattag gagaagcagc tcttaagtta
     5041 tttaggctat gggatattgt ctattttctt gattttatca tttttggaat cctagtctat
     5101 cgtaaactac tccggtggga taaaaaaaca tttcataaaa aatctagctt tgctattaca
     5161 gcactatcag tcatgctttt ctccatcaac ctctttttag cagaaattga tagaccagag
     5221 ctacttacga gaggattttc taatacttat atcgtcagag cccttggttt acctgctttc
     5281 cttggttaca atgctaatca aacctacaat gcccacaaga atcgttctgg agcatcagct
     5341 acagatttgg agcctgtgaa acaatatatt acagaacatt atgctcaacc gaatgacaac
     5401 tacttcggta ttgccaaagg gcgaaatgtt atctatctcc acctagaaag cttacaacaa
     5461 tttgttattg actataagtt gaatgtcaac ggtgtcgagc acgaagtcac acctttcctc
     5521 aattctctgt atcattctag ctctactctt gccttttcca atttctttaa ccaagttaag
     5581 gcaggtaaga cctctgatgc cgaaaccatg attgaaacct ctctttttgg actcaatcaa
     5641 ggctctttca tggtccaata tggcggaagc aatacccaac aagctgcacc atatattctc
     5701 tctcaaaatg gaggatatac atctgctgta ttccacggta atgcagccag tttctggaat
     5761 aggaacaaca cctataaaca atggggttac aattacttct ttgatcaatc ctatttctct
     5821 aaagccactg ctgaaaattc attccaatac ggtctgaatg ataaaatcat gttcgccgac
     5881 tctatccaat atctggaaca tttacagcaa cccttctata ccaaatttat cacagtttcc
     5941 aaccactacc cctatacaac cagcttgatt ggggatgaga ttggctttcc attagccaat
     6001 acagatgatg aaaccattaa tggctatttt gctactgcca actatctcga tgcgtccata
     6061 aaggcatttt tcgattactt aaaagcgact ggcttgtacg aaaactccat catcgtcctc
     6121 tatggtgatc actatggaat ttctaattca cgtaatcctg acttggcacc gttgattgga
     6181 aaagattctc agacttggtc agattttgac aatgccatga tgcaacgtgt tccatatatg
     6241 attgttgtac ctggaatgac caacggtggg atttttgata cctatggtgg tgagattgat
     6301 gcacttccca ctctcgaaca tcttttagga atcaaatccg ataactatct tcaagttggt
     6361 caagacctac tttccagcca acacagccaa attgtggctc ttcgaactgc tggcagtttt
     6421 atcacaccaa aatacactag ctacgaaggt aaaatctatt atacagaaac tggtcttgaa
     6481 attacaaatc cagatgagac aactcagaaa gaaattgatg caattaaaga agctactgca
     6541 gcacaactgg cgactagcga tgccattcaa actggtgatc ttatccggtt cttcgacaat
     6601 ggattgccag ccttggatcc aagccaatat aactacattg actcattggc tgccgagatt
     6661 gcactagaga ataaacttgg caaacaatca acaagtttgt atagcacaaa cggcaataaa
     6721 tcaacaattg aactatttaa agcaccaagc tacctagaac ttcacggtag tacaactgct
     6781 accagtacac caaatactgg tacaagcact agctcaacaa gtaccagttc aaattcacgg
     6841 taatacacta taaaaggctt agaatcatgt cagttactac attgattcta agccttttta
     6901 tactcaatga aaatcaaaaa cagactaggc gacgcagatg cagatagaac tgaagttcat
     6961 caaatcaagt caacaacgtc tgattttgat tttcgaagag tattagacca ctatttcaaa
     7021 tcactcatat ctatttcatc aaggaaatat aaaatcctca ctaggctctt cctctgttga
     7081 tgatttttta gggatatcta tgggagcagc agtactactt tcagttgctt ttgtatcttc
     7141 tgttgtagtt tcagcttctg ttacagtttc tatatctgtc gcttctggac taggtgtcaa
     7201 caaatcatct aaccagctat ctgcgtcctc ttctccatta gggacatctg gagtattcgc
     7261 caatggattc ccaggcaacc aagtatcacc tggctcaact gtcccatctt tgctatcact
     7321 ttcaacttta tcgaaatgaa tatcgtagac agtatcttcc aaaataggtg tatcccatgc
     7381 tacatttgga ataacaggtt ttgtataatc tcgtttataa cctgtatcac tcttcaaaga
     7441 tggcggaaaa attccagctt ggttcagtgt taaccctttt ctaacaaggt aaacaactgt
     7501 tttactagtt cccaaatgtg tctgaagttg accaccttca cctgctcgaa atgcgagggc
     7561 tacataattc tttggatcat gccaatagta atttggatgt ctacttgggt cattgatgtc
     7621 cgttggattc tccgtattgt tagcaatgta aggtcctata cgaggagccg ttgtcccaaa
     7681 tgctgccaag ctcgttgtgg cactaacata atctgtatct ttttctccac cagtaataac
     7741 tgtcctaaac tcaatgaact cataatcttt gcttgaaacc ggttctggta caacaagtcc
     7801 ttgctcctta gcttgtttcc aagttaagtc agatttcaca aagtatatga accctgctcg
     7861 aatctcacca tttaatttta aacgaccatt ggctatcgca ctatatttaa caggtacata
     7921 gtctgataaa tcaatatttg aaacatcatc acttggagtg taaatatttt tggttggctt
     7981 acgataaaca ttatcaaaat cagcataaaa actttgatgt tctcttgcca tcttgccaga
     8041 ctttggcaaa gtagcattcc aaccgttaaa gacgcgtccc ttttgttgaa gggtctgcgg
     8101 ataggcgatg ttcaaatcct cccaatttaa atctttacga accaaataat aaactctctt
     8161 cgttttttta ccattaacga caaaattacc aaaatcacta tttgtactga atgttactag
     8221 aatgaaattt ttaatatcct gaagaccttc tggacgatac ttttgatgga caccattacg
     8281 tgttaaattt tctgttgaac cttctgtata aaaaggtcca tatactaatt gtttctcaat
     8341 agcaagattt accgtttcag tcttaacatc acgctcagga tcatctggca aactaggatc
     8401 aattttataa acgtgcgcat ggtctccatg gcgtacactc atatagttcc catcaattgt
     8461 aatgtcctca cgttttactt taaaacgctc catgtactgt ttaatgagac gttcttttct
     8521 ctgttcaaga gtttctccat ccttttgttt aagaatacga tcttccggac tttcaaatgg
     8581 cttcgttgta tcaatttcct ttaataaaac aatatgtgtg tgatctccat gcggatactc
     8641 taatccaatt tgaccgtcaa cgatgacttt cttaaaacgt gatggctcta aattcagttc
     8701 ttttgctaag tagtccaatt tggtttgata ctcagtattt tctacctcag gttgtgcctt
     8761 cttatccgat tcaacatttg gtttatactg attcacaaga taagaccatt tactcctttt
     8821 caatgtttca aaatgaataa gatgcaaatg tttcccatgc cttactagaa tcccggtttc
     8881 cgttacaccg ctgatatttt gcccatcaaa caagaaaccg tcactcgttt gaaaatcaat
     8941 ccccggaatc ccttcctgtt ggtgaacgat aggaagagtt ggcgattgac gactatcaga
     9001 ggtcaattgt ctgtgtgtcg tgccacctaa gcttgatttt aaaatatagt gataatgatc
     9061 cccatgccga acagtatagc cattggcatc ctctgcgaca atatctttag gatcaaaaac
     9121 gtattcatct tcgatctgtt tcgagcctga cgataattga cttctcttag gttgagaaga
     9181 atgatttttt tcttgatagt tttcaggaat taaataagcc cattttgtat tctttaaatc
     9241 tgaataaaag aaaaagtgct tatgatcgcc atgattgaca ataattccta aatcagtttt
     9301 ttcctcaatt tgtgactcat cagtgagtaa aaaaacatca tccgtcgcct tatcaatccc
     9361 tggaacttcc tttttcgaat ctgaaggaat ctcttttgat tgctttgatt gcgatgtttc
     9421 tatgcttgtt gttttttgat ggttcgattg ctctctatag ttagacgctt gataagtaac
     9481 caatgctaca gcacaaataa gcgatgctcc tgctacataa agcaaagtct taccttgttt
     9541 catatctatg tttctctcct tatttaccat tattatatta ccgacacgat aaatgtaatc
     9601 aactattgta aaatagcatc accacatcca ctta
//
LOCUS       DBNFCK010000066         9590 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.066, whole genome shotgun sequence.
ACCESSION   DBNFCK010000066 DBNFCK010000000
VERSION     DBNFCK010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9590
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.066"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            13..1359
                     /gene="gdhA"
                     /locus_tag="AC1PIW_001290"
     CDS             13..1359
                     /gene="gdhA"
                     /locus_tag="AC1PIW_001290"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HIP6606225.1"
                     /translation="MSNAKAYIQASFEAVKARNPHETEFLQAVEELFSTLEPVFEAHP
                     EYIEENILARIVEPERIISFRVPWTDKDGNVQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQSILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEIMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYMYGQYKRLRQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     FTDNMLAANGKSFKDQTVLISGSGNVAQYAVQKATELGAKVISVSDSNGYIIDETGID
                     FDLLVDIKEKRRARLTEYAAEKSTAKYFKGSVWNYDGKADIALPCATQNEINGKQAAA
                     LVKNGVYCVAEGANMPSDLDAIKVYKENGVLYGLAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMANIFNTAKETAEKYDLGTDYLAGANIAAFEQIADSMIAQGLV"
     gene            complement(1595..2182)
                     /locus_tag="AC1PIW_001291"
     CDS             complement(1595..2182)
                     /locus_tag="AC1PIW_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HIP6606226.1"
                     /translation="MQTKDFVVENGQMYYKKKPLHKQPLFWTTIAGAVLSFILGVTCF
                     IFMIGLSSANFDNWDTGSDGYIDETVEYTEYQVGDSVDFSNGLHVTVTSMGKDDSIEL
                     VDSYYSTAYVVEMEVENSTAKELYFDEYYFSLMDPVSQIPFTLDLRTYDVNLVEKLKP
                     GEKVQVKLIYGIDNETNFGFTYEDAMWTELVAEGI"
     gene            complement(2193..3041)
                     /locus_tag="AC1PIW_001292"
     CDS             complement(2193..3041)
                     /locus_tag="AC1PIW_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIP6606227.1"
                     /translation="MNDFITKYLSQFNIEAIVTAGLNKVLSLVLLFVAFYIIKKIAKA
                     SVKKVLVPSLKVSTQDIGRQKTISRLVESILNYLLYFILIYCILSILGLPVSSLLAGA
                     GIAGVAVGLGAQGFLSDLVNGFFILIERQFDVGDVVKLTNGPITISGTIVSMGIRTTQ
                     VRDADGTLHFIPNRNILVVSNQSRGDMRAQIDIPLKFNTDLEQVYRVIEEVNLREISN
                     FDQITGVTVLGPQNAISGQFVFRVNLFVANGQQNKIYHQFFGFYQDALRQAGIDLPSS
                     GPTVLK"
     gene            complement(3106..4440)
                     /locus_tag="AC1PIW_001293"
     CDS             complement(3106..4440)
                     /locus_tag="AC1PIW_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HIP6606228.1"
                     /translation="MLELFKAINNLVWGPPLLLLLVGTGVYFTLRLGVFQIGKLPTAF
                     RLIFSSDQSGQGDVSSFAALCTALAATVGTGNIVGVATAITTGGPGALFWMWVAAFFG
                     MATKYAEGFLAIKYRTKDANGQAAGGPMHYITLGMGQKWKPLAVFFAISGVLVALLGI
                     GTFSQVNSITASLETSFGLEPPLVSVITAISIAFVVFGGIEKISDVSTKIVPFMAILY
                     ILASITVLAVHWDQLLPTLALVFKSAFTPAAAVGGFAGATVQQAIQRGIARGVFSNES
                     GLGSAPIAAAAAKSDNPVEQGLISMTGTFIDTLIICTLTGFTILVTDQWSVEGLAGAP
                     LTQAAFATVFGNTGSIALTISLVLFAFTTILGWSYYGERCIEFLFGTKSILPYRLVFV
                     AMVALGGFLKLDLIWTIADIVNGLMALPNLIALLALSPVIIKETRQYFAKKK"
     regulatory      complement(4547..4651)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(4864..5496)
                     /locus_tag="AC1PIW_001294"
     CDS             complement(4864..5496)
                     /locus_tag="AC1PIW_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6606229.1"
                     /translation="MFSVQSIQKKFGKRSLFSDVTLDLQAGKVYALIGASGSGKTTLL
                     NILAKLESYDAGDILYKGKDLKKLKPHLFFREELGYLFQNFGLLESQTIKENLDLGLI
                     SQKLSKDERKSRQEAALDAVGLSYLDLSQPIYELSGGEAQRVALAKVILKNPPLILAD
                     EPTAALDPKSSQEIMDILLSLKSPDRLIVIATHNPLIWERADLIIKMEDL"
     gene            complement(5498..7483)
                     /locus_tag="AC1PIW_001295"
     CDS             complement(5498..7483)
                     /locus_tag="AC1PIW_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HIP6606230.1"
                     /translation="MKGKFVLVSTIILAVFMIWLGVSQKETMLVHYYPSVTTLSVSED
                     VTYSDVRQRLEDYSQQTDSVIARRVIEPSKSGGRTFSYDNFSQSPLPRGLEEFQASEK
                     VESALLTKYFIFQGKATVEELRSLLVSLGLDEVQIRKPSTIATLLVFLTQGGQFLAVL
                     VFLITYMALVVIANVRQLRTAGIRLIAGDSRWHLFLLSLQENAKEIALTIPFAVLPAV
                     GLAYLVGLDGYSVYYLVAALVGYHFLLGLIVLFFAATFTLAIRTYHFLPLLKGKMPLQ
                     GILTIMVMGQMLALLVVSFGMAQTVYYSGIWQEYQAGAQQWENEGDYYSLAWNISADG
                     RSGLNSPENWYPLLRQALEEDGALFVKSNLNAYLMGFQLEDGTRLDSYHPAGNTLYVS
                     PNYLQIQDVDLAEGEVALPLQEGEFQLLLPEKLRPESDAYLHLYQDYINRMVRPANQA
                     RSATIKGKVAYLKNGQKQFIYNHRSGQHVQYLTDPILVVLAPSSLGKESADFWLNEVD
                     SSMLFKNRDKLLRELKARNLFQFVNEVRSSSSLFTELLDRIRIETISTSMGAILGIVT
                     SIVLFNTMNLLYFEEFKREIVIKEIAGMGFLGIHKKYLTLQLLSLLLALGASMVVTGH
                     IFISSISFALFMVNALCLLRWQARKEGAWNVRILKGA"
     gene            complement(7503..9488)
                     /locus_tag="AC1PIW_001296"
     CDS             complement(7503..9488)
                     /locus_tag="AC1PIW_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HIP6606231.1"
                     /translation="MKKVFTYLSTFSVCLFCIWLVSLQLDSLIYHYYPSVSILSRGEE
                     VTYEEVSDALTNLAEKENSLIAKTITETDDSGRTTTSYDVFGKGELPQGLVKADRLVA
                     SQANLAENYYVVKGNLDIKVVENVLQSLGLVDSYIYRPTLLNSLSLFLGRGAPSLGLI
                     IFILTNLALTVVGQIVYLRSAGIEMLSGVRSWQIASRFLRVDGRDIALATGGALVFGL
                     IAILILGWPMVYYRFVAIGIAFYACLLFFLSLSTSIGFMIALNISQLQKLIKGALPLK
                     AIFAILLVGQCLAVCIISIGASRTLTYVGVLHTLTIGEKAWQERQDVFSLSLNREAFI
                     ADEAMAQQQRMAWSHVILESYAKNNVMLVQHHLRHNTIKEHTIIESSKEIPLERVLYV
                     TPNYIQSEGGLYDFNQLEEIKQLGKGEIALLLPKSLQNDASVYQAYFEDMVGKLLADG
                     EGSISLHSNVYYISDEKRWFIYNHTPINYEQFLQAPLIVVLSPESFEETSYFWENALP
                     DFVFFKDKEMLEQLLDKYNLQNTIGSLLSSKQHYNTLRKNVQLEILMTLSPTILGIFT
                     SILLFNTMNLLYFETFKREIAIKRIAGMGFIELHGSYLGEQVGSALIGMGLAMFMTKS
                     VIVSLGVVVALLINSWMLLNNQAKREEKIQLSVLNGR"
BASE COUNT         2999 a         2197 c         1785 g         2609 t
ORIGIN      
        1 tcaggtgatc ccatgtcaaa tgccaaagct tacatccaag cttcttttga agcagtcaaa
       61 gcccgcaacc cacatgaaac agaattcctc caagctgtag aagagctctt ctctacactt
      121 gagcctgttt ttgaagcaca cccagaatac atcgaagaaa acatcttggc tcgtatcgtt
      181 gagcctgagc gtatcatcag cttccgtgtt ccatggacag ataaagatgg aaatgttcaa
      241 gtcaaccgtg gctaccgtgt tcagttcaac tcagctgtag gtccttataa aggcggtctt
      301 cgcttccacc caactgtaaa ccaatccatc ttgaagttcc tcggttttga gcaaatcttc
      361 aaaaacgtct tgactggtct tccaatcggc ggtggtaaag gtggttcaga ctttgatcct
      421 aaaggaaaaa ctgatgctga aatcatgcgc ttctgccaaa gcttcatgac tgaattgcaa
      481 aaacacatcg gaccttcact tgacgtccct gctggtgaca tcggtgtcgg tggtcgtgag
      541 atcggttaca tgtacggtca atacaaacgc ctccgccagt ttgatgcagg tgtcttgact
      601 ggtaaacctc ttggcttcgg tggttcattg atccgcccag aagcaactgg ttacggtttg
      661 gtttacttca ctgataacat gttggcagca aacggtaaat ccttcaaaga ccaaactgtc
      721 cttatctcag gttctggtaa cgttgcccaa tatgctgttc aaaaagcgac tgaacttggt
      781 gcaaaagtta tttctgtttc agactcaaat ggttacatca ttgacgaaac tggtatcgac
      841 ttcgacctct tggtggacat caaagaaaaa cgccgcgctc gtttgacaga atacgctgca
      901 gaaaaatcaa ctgctaagta cttcaaaggt tctgtatgga actacgatgg caaggctgat
      961 attgcccttc catgtgcgac tcaaaatgag atcaacggca aacaagctgc tgcccttgta
     1021 aaaaatggcg tgtactgtgt ggctgaaggt gccaacatgc catctgacct tgatgccatc
     1081 aaagtctaca aggaaaatgg cgttctctac ggactcgcaa aagctgccaa cgctggtggt
     1141 gtagctgtat ctgcccttga aatgagtcaa aacagccttc gcttgtcatg gactcgtgaa
     1201 gaagtagacg gccgtcttaa agacatcatg gccaacatct tcaacacagc caaagaaact
     1261 gctgaaaaat acgaccttgg tacagactac cttgcaggtg ctaacatcgc agcctttgaa
     1321 caaattgcgg atagcatgat tgcccaaggt ttggtataag cttactttta gtttactaaa
     1381 ctataataaa aacgccccca aaattgacgt gctaattttg ggggtatttt tcattaaaag
     1441 aagaggagaa cagtggataa acatattctc cttttttaaa tgtaaaaata acagataagt
     1501 cggatatagt acaattacca agccgaacaa tctgaaccaa ggggcgttat aaaaccgcaa
     1561 aattgtgcaa tatttaaaac tttttctaca gcaattagat accttctgcg actaattccg
     1621 tccacatggc atcttcgtag gtaaaaccaa agtttgtttc attatctata ccgtagataa
     1681 gttttacttg tactttctct cctggcttca acttttcaac tagattcaca tcataggtcc
     1741 gcaaatccag ggtaaaggga atctgactaa cgggatccat gagactgaaa tagtattcat
     1801 caaaatagag ctcttttgcg gtcgaatttt caacttccat ttcaacaaca taagctgtgc
     1861 tgtagtagct atccactagt tctatgctat cgtctttgcc catagatgtt actgttacat
     1921 gaagaccgtt agaaaaatca actgagtcac caacttgata ctctgtgtat tccacagtct
     1981 catcaatgta tccgtcacta cctgtatccc aattatcaaa atttgctgag cttaggccga
     2041 tcataaaaat aaaacaggtt actcctagga tgaaggagag gacggcacca gcaatggtcg
     2101 tccaaaagag aggctgtttg tgcaagggtt tcttcttata atacatctgc ccattttcaa
     2161 cgacaaaatc ttttgtttgc ataataaact ccttatttta aaacagttgg cccacttgaa
     2221 ggtaaatcta ttcctgcttg acgcagagca tcttgataaa aaccaaagaa ttgatgatag
     2281 atcttgttct gttgaccgtt ggctacgaaa agattaacac gaaatacaaa ctgtcccgaa
     2341 atagcgtttt ggggacctaa tacagtcaca cctgtaatct ggtcaaaatt gctgatttct
     2401 ctcaaattga cctcttctat tacgcgatag acttgttcca aatcggtatt aaatttgagc
     2461 ggaatatcga tttgagcacg catgtcccct cttgactgat tggacaccac tagaatattg
     2521 cgattgggaa taaaatgcaa tgtcccatct gcatcacgta cttgtgttgt tcggattccc
     2581 atgctaacga tggttcctga aatggtaatg ggcccgttgg ttaacttaac cacatctccg
     2641 acgtcaaact gtcgttcaat taagataaag aatccattga ccaagtctga gagaaaacct
     2701 tgtgcgccca gcccaacagc taccccagca atcccggctc ctgcaagaag actggatacc
     2761 ggtaaaccga ggatactcaa aatacagtag ataaggataa agtagagtag gtaatttagg
     2821 atgctttcca ccagacgact gatggtcttc tgacgaccaa tgtcttgggt agataccttt
     2881 agagagggaa ccaaaacctt tttcacagaa gctttggcta tcttcttgat aatgtaaaat
     2941 gcaacaaaga gtaaaacaag cgataaaacc ttatttagac ctgctgtcac aatggcctca
     3001 atattaaatt gagacaaata cttggtaata aaatcattca tgagataatt atagcagaaa
     3061 acaccctaaa agaacaaaaa gacagccaag tggctgactt ttatcttatt tcttctttgc
     3121 aaaatactgg cgtgtctcct tgatgataac aggagagaga gctagtaagg cgatcaagtt
     3181 tggtagtgcc atgagcccgt tcacaatatc tgcgatggtc caaatcaagt ctagtttgag
     3241 gaaaccaccc aaggcaacca tagctacaaa gaccagacgg taaggcaaga tagacttggt
     3301 gccaaagagg aattcgatac agcgttcacc atagtaagac catccgagaa tagttgtaaa
     3361 agcaaaaagt accaaactaa tcgtcaaggc aatagatcct gtattgccaa aaactgttgc
     3421 aaaagctgcc tgagtaagag gagcacctgc caagccctct accgaccact ggtcagttac
     3481 tagaatcgta aaacccgtta aggtacagat gataagtgta tcaataaagg tacccgtcat
     3541 agaaatcaag ccctgttcaa ctggattatc agacttggca gccgcagcag caataggcgc
     3601 cgaacccaaa ccagattcat ttgagaaaac cccacgcgca atcccacgct gaattgcttg
     3661 ttgcacagtc gcacctgcaa aaccgcccac agccgctgct ggagtaaagg cagatttgaa
     3721 aactaaggcc agggttggca agagttgatc ccaatgcacg gccaagacag taatacttgc
     3781 taagatatac aaaatcgcca tgaaaggaac aattttggtc gaaacgtcag agattttttc
     3841 aatcccacca aaaacaacga aggcaattga aatagccgtt attacactga caagtggcgg
     3901 ttccaatcca aaactcgttt ccaaagaagc agtgatggaa ttaacttgcg aaaaagttcc
     3961 aatccccaag agagctacta atacacctga aatagcaaag aaaactgcca agggtttcca
     4021 cttctgtccc atccccaagg tgatataatg cataggtcca cctgccgctt gaccgttggc
     4081 atccttggtt cggtatttaa tcgctaaaaa tccttccgca tacttggttg ccattccgaa
     4141 aaaggccgca acccacatcc agaaaagagc cccaggacca cctgtagtaa tagctgtcgc
     4201 aactccgacg atatttcctg taccaactgt cgctgctaaa gccgtacaca gagccgcaaa
     4261 actggacaca tctccctgac cagactggtc actggagaaa atcagacgaa aagccgtcgg
     4321 caatttgcca atctgaaata ctcccaaccg tagggtaaaa tagacacccg ttccgaccaa
     4381 tagtaacaag aggggcggtc cccaaacaag attgttgata gccttaaata gttctaacat
     4441 agttcctcct aagcgtttct aacccgataa agctgccgga aaaacaaaaa gaggctgaac
     4501 acttcaacct ctagaatcaa cgtgaaaaat acagaaaaag cctatttttc aacgctctgt
     4561 ccttttacct gagagtttca gtagctctac ttttccaagc aggactacct tgccccttcg
     4621 gtgccaaaat ggtctctcca gagttccgtc cattgaacag tcagtttccc ttctacccaa
     4681 tatcaaccgg ttattcagtt ttatctagtt ttattttaat ctaatttcac gaaaaaatca
     4741 agaatatttt ctgatactca atgaaaatca aaagtagcct aggaaacgaa gccgaagata
     4801 gaactggagt tcatcaaggt aagttgacaa cggataattt tgattttcga agagtatgaa
     4861 tttttacaag tcttccattt ttatgataag gtctgctcgc tcccaaatca aaggattatg
     4921 ggtcgcaatc acaattaacc gatctgggct cttcaaggat aacaaaatat ccataatttc
     4981 ttgggagctc ttaggatcca aggcagccgt cggctcatct gctaaaatca atggaggatt
     5041 tttcaggatg actttagcaa gagctacccg ctgggcttcc cctcctgaga gttcatagat
     5101 aggctgggac aggtccagat aagacagacc aactgcatca agtgccgctt cctgcctcga
     5161 tttcctttcg tctttggaca atttctgact aatcagtccc aaatccagat tttctttgat
     5221 tgtctgactt tctaaaagac caaaattctg aaagagatag cctaattcct ccctaaaaaa
     5281 gagatggggc ttcaactttt tcaaatcctt tcccttgtag agaatgtcac ctgcatcata
     5341 ggactccaac ttggctaaaa tattcagcaa ggtcgttttt ccgctaccac tggcacctat
     5401 caaggcatag acctttccag cctgcaagtc tagtgttaca tcagaaaaca agctgcgctt
     5461 cccaaatttc ttttgaattg attggacaga aaacatacta ggctcctttc aaaatacgga
     5521 cattccaagc tccttctttc ctcgcctgcc acctcaataa acagagggca ttgaccataa
     5581 acaaggcaaa actgatgcta cttataaaaa tgtgacctgt tactaccata cttgctccaa
     5641 gagccaacaa gagagaaagc agttgaagcg ttaggtattt cttatggatt cccaagaaac
     5701 ccatccctgc aatttccttg atgacaatct cacgtttaaa ttcttcaaag tatagaagat
     5761 tcatggtatt aaacaggaca attgatgtca caatccctaa aatggcaccc atagatgttg
     5821 aaatggtctc tatccgaata cgatctaaaa gttccgtaaa gagggaagaa ctacttctca
     5881 cttcattgac aaactgaaac aaatttcttg ccttcaattc acgtaaaagt ttatctctgt
     5941 ttttaaatag catactgcta tctacttcat tcaaccaaaa gtcagcagat tcttttccca
     6001 gacttgacgg tgccagcaca actaaaatag ggtctgtcag gtattgcaca tgttgaccag
     6061 aacgatgatt gtagataaac tgcttctgcc cattttttaa ataggcaact tttcccttaa
     6121 tggtagctga gcgtgcttga ttagcaggtc taaccatacg attgatataa tcttggtaaa
     6181 gatggagata agcatcgctt tcagggcgca atttttcagg caacagaagt tgaaattctc
     6241 cttcttgtaa agggagcgct acttctcctt ctgctaagtc tacatcctgt atctgcaaat
     6301 aatttggact aacgtagagg gtgtttccag ctggatggta gctgtcaagg cgtgttccat
     6361 cttccagttg gaagcccatt aaataggcat tcaaattact tttgacaaag agtgcgccgt
     6421 cttcctccaa agcttgcctt agtaaaggat accaattttc aggcgaattt agtcctgagc
     6481 gaccatccgc agaaatattc caagctaaac tatagtaatc cccttcattc tcccactgct
     6541 gggcacctgc ttgatattcc tgccaaatcc ctgaataata gacggtttga gccatcccaa
     6601 atgacaccac caatagagcc agcatttgtc ccataaccat aatggtcagg atcccttgca
     6661 ggggcatctt cccttttaac aagggtaaaa agtgataggt tcgaatggcc aaggtaaatg
     6721 tagcagcgaa aaaaaggaca atcaaaccaa gcaagaaatg atagcccact agagctgcga
     6781 ccagatagta gacagaatag ccatctaatc caacgaggta ggccagtcca actgctggaa
     6841 gaaccgcaaa tgggatagtt agagctatct ctttcgcatt ttcctgtaga gatagtagaa
     6901 ataaatgcca gcgcgaatcc cctgcaatca aacggatacc tgctgtacgc aactgcctta
     6961 cattagcaat cacgaccaaa gccatatacg taataagaaa gaccagtacg gctaagaatt
     7021 gaccaccttg ggtcagaaat acgagtaaag tagcaatagt cgaaggcttc cgaatctgaa
     7081 cttcatcaag tccaagcgaa acaagaagag accgaagctc ctctaccgtc gctttccctt
     7141 ggaaaataaa atatttggtt aaaagtgcag actccacctt ttctgatgcc tgaaattcct
     7201 ctagtcctct tggtagggga gattgactaa aattatcata agaaaaggta cgtccacctg
     7261 atttacttgg ctcaatcact cgtctggcaa tgacactatc cgtctgttga gaatagtctt
     7321 ctaatcgctg tcgtacatct gaatatgtca cgtcctcaga taccgacaaa gtagtcacag
     7381 aaggataata atgaactagc atagtctcct tttgactgac gcccaaccaa atcataaaga
     7441 cggctagaat gatcgtcgat acaagtacaa attttccttt cattctaact tcacctcatt
     7501 aactatctgc catttaatac actcaactgg attttttctt cccttttcgc ttgattgttc
     7561 agtaacatcc aactatttat caacaaagcc acaactactc ctaggctaac aataactgac
     7621 tttgtcataa acattgccaa acccatccca ataagagcgg agccgacttg ttctcctaga
     7681 taagaaccat gcaattcgat aaaacccatc cctgctatcc gcttgatggc tatttctctt
     7741 ttgaaggtct caaagtatag aagattcatc gtattgaaca atagtatgga agtgaatatt
     7801 ccaaggattg tcggagatag ggtcataaga atttcaagtt gaacattttt tctcagggtg
     7861 ttgtaatgtt gcttgctaga taacaagcta cctattgtgt tttgtaaatt gtatttgtcc
     7921 aacagttgtt ccagcatctc tttatctttg aaaaatacaa aatctggtaa cgcattttcc
     7981 caaaaatagg acgtttcttc aaaactttca ggtgatagaa caacaatcaa cggagcctgt
     8041 aaaaattgct cataatttat cggggtatga ttgtaaatga accatcgctt ctcatcggaa
     8101 atgtaataaa catttgaatg caaacttatg gatccttctc catccgcaag caattttcct
     8161 accatgtcct caaaatacgc ttggtaaaca ctcgcatcat tttgcaaact ctttggtaat
     8221 aataatgcaa tttctccttt acctaattgc tttatctctt ccagctgatt gaaatcgtac
     8281 agtccacctt ctgattgtat ataattcggc gtcacataca gcaccctttc tagaggaatt
     8341 tccttgctgg attcaatgat ggtatgttct tttattgtgt tatgccgtaa atggtgctgg
     8401 actaacatta cattattttt cgcatatgat tctaaaatta catgagacca agccattctt
     8461 tgttgctggg ccatcgcttc atcagctatg aatgcttcac gattcaaaga cagactaaaa
     8521 acatcttgtc tttcttgcca agccttttct cctatcgtta gtgtatggag aactccaaca
     8581 tatgtcaacg ttcgactagc accaatagag atgatgcaaa ccgctaaaca ctgacctact
     8641 agtaaaatag caaaaatcgc cttgagaggc aaagctccct taattagctt ttgtaactga
     8701 gaaatgttta atgcaatcat gaatccaata cttgtcgata aagataggaa aaaaagcaaa
     8761 caagcataaa aggcgatacc gattgctaca aaacgataat ataccattgg ccaacccaaa
     8821 attaaaatag caattaatcc aaaaacaagc gctcctcctg tagccaatgc tatatctctt
     8881 ccatccactc ttaaaaaacg actagcaatc tgccaactcc tcacccctga aagcatctct
     8941 atacctgccg accgcaagta gacaatctgc ccaactactg tcaatgcaag attggtgagt
     9001 ataaagataa taaggcctaa gctcggagct cccctaccaa gaaatagact taaactgttt
     9061 aatagggtcg gtctataaat atagctatcc accaatccca gtgactgtaa gacgttttct
     9121 accactttga tgtccagatt accttttaca acatagtaat tttcagctaa gttggcctga
     9181 ctagctacta acctatccgc tttgaccaag ccctgtggaa gctccccctt cccaaaaaca
     9241 tcataactgg tagtagttcg cccactatca tctgtttctg ttattgtctt agcaattaga
     9301 ctattttcct tttcagctaa atttgtcagg gcatccgaca cttcttcata ggtcacttct
     9361 tcccctcgac ttaatatgga aaccgatgga tagtaatgat aaattaaaga atcgagttgc
     9421 agactcacta accatataca gaagagacag acgctaaaag tagataaata agtaaatact
     9481 tttttcataa cgaaccccat ttgcacaaaa aggaggtgga ctccaccacc tccctatcat
     9541 tagaacccgt aaccaaaatc aacatgtttt caaacgttcg ttgatatagc
//
LOCUS       DBNFCK010000067         9469 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.067, whole genome shotgun sequence.
ACCESSION   DBNFCK010000067 DBNFCK010000000
VERSION     DBNFCK010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9469
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.067"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(12..551)
                     /locus_tag="AC1PIW_001297"
     CDS             complement(12..551)
                     /locus_tag="AC1PIW_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1 family glyoxalase III"
                     /protein_id="HIP6606232.1"
                     /translation="MTRVAVVLADGFEEIEALASVDVFRRAHFDCQIVGLDSHEVEGS
                     HGIRVQTDQVFDGDLSSFDLIVLPGGMPGSVHLRDSESLITELQRAVASGKSVAAICA
                     APIVLDKAGLLDSRHYTCFPGKEKDIPSGIHLEEDVVVDGPIITSRGAGTSLDFAYKL
                     VDLFGGDGESLAQTMVYHS"
     gene            complement(565..1794)
                     /locus_tag="AC1PIW_001298"
     CDS             complement(565..1794)
                     /locus_tag="AC1PIW_001298"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIP6606233.1"
                     /translation="MRKLMKNRGTIDSRVDYSLILPVFFLLVVGIISLYIAVSQDYPD
                     NVVQMVGQQIAWIGIGTVAAFLVMFFSTKFLWQITPYLYVLGLGLMVLPLFFYSPDLV
                     ASTGAKNWVTIGGMTLFQPSEFMKIAYIIMLSRVIVTFHKYYPNRKIREDFMLIGYMT
                     LFTIPVLILLALQKDLGTSLVFVAIFSGMLLLSGVSWKILLPTALTGIVLVGGFMLIF
                     ISPGGTTFLHNLGMDTYKINRIAAWLDPFKNAQSTTYQQAQSLIAIGSGGLKGLGFNK
                     TNLLIPVRESDMIFTVIGEDFGFIGGTVLIGLYLLLIYRMLRVTLKSNNRYYTYISTG
                     YIMMLLFHVFENVGAATGLLPLTGIPLPFISQGGSSMVSNLIGVGLVLSMGYQSRLAD
                     EKETNRSRRYRKITIKR"
     gene            complement(2243..2485)
                     /locus_tag="AC1PIW_001299"
     CDS             complement(2243..2485)
                     /locus_tag="AC1PIW_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HIP6606234.1"
                     /translation="MKKDIHPEYRTVVFMDTTTGYKFLSGSTKKSNETVEFEGETYPL
                     IRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(2604..3632)
                     /locus_tag="AC1PIW_001300"
     CDS             complement(2604..3632)
                     /locus_tag="AC1PIW_001300"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIP6606235.1"
                     /translation="MQASSRSLRLMGTIIETKIWHPDAEPILDQVEELLYLYKDRFSA
                     NDLTSELMEVNLNAGVQAVPVADDLYELIKLGKEHSLAEGSFLNITIGPLVQSWRIGF
                     SDAKLPTQEMISEKLQLINPRDIELDDEEQTVYLKKEGMAIDLGALAKGYVADKIVDF
                     LKRIGVAAGLINLGGNVLTFGQAPHNADGYWRIGIQDPQKPRGENALVLKIGEESVVT
                     SGIYERTLTVNGKTYHHILSPETGYPMESQLASLTIVSKQSVDGEIWTTRLFGQEITR
                     IYKMVEETDGIEAVLIGLDGRILVTSGLSEKVLAGEERISDSLGSPSRGGFGARVTSD
                     LASGASVL"
     gene            complement(3619..4563)
                     /locus_tag="AC1PIW_001301"
     CDS             complement(3619..4563)
                     /locus_tag="AC1PIW_001301"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIP6606236.1"
                     /translation="MTIYSKIIEKIHEYDTIIIHRHKRPDPDAIGSQVGLQKLLQKSF
                     PGKTIKVTGFEEPNLAWMAGMDEVADQDYQGALVIVTDTANTARVDDDRYAQGDFLIK
                     IDHHPNEEPYGDLLWVNTEASSCSEMITELAIQCQLELDGEIARLLYAGIVGDTGRFL
                     YPATSSRTFEIVARLRQEDFDFARMSRQMDTIDFKIAKLTGYVYDNLEVDEHGAARVI
                     LTQDILEKYGVTDADTSFIVGAPGRIGIVQSWGIFVKQTDGNYRVRLRSKYLPINEIA
                     KRHDGGGHPLASGANSYSLSENEQIYQEIQEVVRNASK"
     gene            4658..5119
                     /locus_tag="AC1PIW_001302"
     CDS             4658..5119
                     /locus_tag="AC1PIW_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HIP6606237.1"
                     /translation="MALAKIVYASMTGNTEEIADIVASKLEELGLEVQVHECTTIETE
                     EILDADLIVVASYTYSYGGDGELPDEIVDFYADLADLDLTGKVYGVCGSGDTFYDDFC
                     SAVDDFDVMLGSRGATKGAENVKVDLAAEDEDIVNLEQFATDLVAKLDTMN"
     gene            5260..5529
                     /gene="rpsN"
                     /locus_tag="AC1PIW_001303"
     CDS             5260..5529
                     /gene="rpsN"
                     /locus_tag="AC1PIW_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HIP6606238.1"
                     /translation="MAKKSKMARYQRQLELIERYADLRKSLKEKGDYQALRKLPRDSN
                     PNRLKYRDKTDGRPHAYMRKFGVSRITFRELAHLGQLPGVKKASW"
     gene            5611..5889
                     /locus_tag="AC1PIW_001304"
     CDS             5611..5889
                     /locus_tag="AC1PIW_001304"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HIP6606239.1"
                     /translation="MNLDCIREQINTIDSQLVELLEKRMELVDQVTAYKRATGKPVLD
                     TSRENAVLERVGKLVQKDDYRSAIQATFSDIMAQSRTYQSSKLANHEQ"
     gene            5879..7093
                     /locus_tag="AC1PIW_001305"
     CDS             5879..7093
                     /locus_tag="AC1PIW_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HIP6606240.1"
                     /translation="MSNKMKHAIQLLFFSSLIGVGAGIITTLFGKILLGIGDLRSEYF
                     TYLIPFLPLAGVLIVFIYQKWGREVQAGMGLVFKAGQGEKAQISPVLIPLIISTTWLS
                     HLVGASVGREGVAVQLGASLSHWLQKHGFTHLPKDMITKIGMAAGFAGLFQTPWAAGF
                     FAIEVLIVGQYSWTSLPYCLVAAFTASTTSHLLGLEKFSHTIATTSFYFTDSFKWLFI
                     ALCFGFIGNLFALFLAQAKSISTRWLPNPYIKIAIMGVGLTVLLFFFHQGRYTGLGTN
                     LIDASLAGEQVFAFDWLLKLLLTCLCLAAGFQGGEVTPLFAIGASSGAVLAGLLGLPT
                     ELVAALGYCAVFGTATNTLLAPLFISYEVFGANILPYAIPVLAIAYLINRKQTIYGQQ
                     LRKFNNAKKPII"
     gene            complement(7114..7185)
                     /locus_tag="AC1PIW_001306"
     tRNA            complement(7114..7185)
                     /locus_tag="AC1PIW_001306"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7150..7152),aa:Arg,seq:cct)
     gene            complement(7381..9405)
                     /locus_tag="AC1PIW_001307"
     CDS             complement(7381..9405)
                     /locus_tag="AC1PIW_001307"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-anchored 5'-nucleotidase"
                     /protein_id="HIP6606241.1"
                     /translation="MKKNIRLKSSILALVAGFSVIATQAVLADELAVQIMGVNDFHGA
                     LDMTGTARLEGETVRNAGTAALLDAYMDDSQAEFEETAAETETPAESIRVQAGDMVGA
                     SPSNSGLLQDEPTVKVFNKMDVEYGTLGNHEFDEGLDEYNRIMTGEAPKKGQFNEIVD
                     NYTREAAKQEIVIANVIDKETGEIPYGWKPYAIKTIPVNDKEAKIGFIGVVTTEIPNL
                     VLKKNYEQYTFLNEAETIAKYARELAEKGVNAIVVLAHVPATSKDGVAAGEAADMIAK
                     LNEIYPEHSVDLVFAGHNHVYTNGTTGKTLIVQATSQGKAYADVRAVYDTDIADFKAV
                     PTAKIIAVAPGQKTPSPEIQAIVDEANTIVKKVTEQKIATASQATDISREVNEFKESA
                     VGNLVTSAQLAIAKKSGYDVDFAMTNDGGIRADLKVQEDGTVTWGAAQAVQPFGNILQ
                     VVQMTGEQIYTALNQQYDEGEKYFLQMSGIKYIYTKADNPTEENPYKVVKAFKEDGTE
                     IVPTETYTLVINDFLFGGGDGFSIFKEAKLIGAINPDTEVFVEYLTDLEKAGQTISAT
                     IPGRKAFVEKYVEEPKAEEKEDNAGTTTDVKTPEKANDGGDSVTNQKATEQPAPSGSM
                     APISNKKTEKASGNQTLPNTGQEALGSLLISLGGLVSLGMAVSVRRKEGE"
BASE COUNT         2706 a         2112 c         1752 g         2899 t
ORIGIN      
        1 tcttcattac tttaactatg ataaaccatt gtttgagcta ggctttcccc atctccacca
       61 aataaatcaa ccaatttata ggcgaaatcc aaacttgttc cagcacctcg gctagtgata
      121 attggaccat caacgaccac atcctcttcc agatgaatac ctgacggaat gtctttttct
      181 ttccctggga aacatgtata gtgacgacta tctaacaaac cagccttatc cagaacaatt
      241 ggtgccgcac aaattgctgc tactgatttt cctgatgcaa ccgctctttg caattccgta
      301 atcagtgact ctgaatctct aaggtgaaca gaacctggca ttcctcctgg taaaacaatc
      361 agatcaaagg aagacaggtc cccatcaaag acttggtcag tttgaacacg aataccgtgc
      421 gatccctcca cttcatgact atctaaacca acaatctgac agtcaaaatg agctcgacgg
      481 aatacatcta cagaggccaa tgcttcgatt tcttcaaaac catcggctaa aacaaccgca
      541 actcttgtca tataaaccaa ctttctaacg ttttatcgta atttttctat agcgacgtga
      601 tcgatttgtt tccttctcat cagctagtcg tgactgatac cccattgaca gcactagacc
      661 aacaccaatc agatttgaaa ccatggacga gccaccttgg gagataaacg gcaaaggaat
      721 acctgttaaa ggtaacaaac cagttgcagc gccaacattt tcaaacacat ggaacaagag
      781 catcatgata tagccagttg atatgtaggt gtagtatcga ttgtttgact tcaacgttac
      841 ccgcagcatt cgataaatga gcaacaagta tagtccaatc aataccgtcc caccaataaa
      901 accaaagtcc tctccaatca ccgtaaaaat catatcactt tctcgaacag gtataagaag
      961 atttgtttta ttgaaaccta gtccttttaa gccaccactt ccgatagcta tcaaactttg
     1021 agcttgctgg taggtcgttg attgggcatt tttaaaggga tcaagccagg ctgcaatacg
     1081 attgatttta taagtatcca tccccaaatt gtggagaaat gttgttccac caggagaaat
     1141 aaaaatcagc ataaatccgc caaccaaaac gatacctgtt aaggcagtcg gtaatagaat
     1201 tttccaagaa actcctgata atagaagcat tccgctaaag attgctacaa atactaagga
     1261 agttcctaaa tctttctgta aggccaacaa aatcaggaca gggatagtaa acagtgtcat
     1321 ataaccaatc aacataaaat cctcacgaat ttttcgattt ggataatatt tatgaaaggt
     1381 gacaatcacc cttgacaaca taatgatgta ggctattttc ataaattcgg atggttgaaa
     1441 aagagtcatt ccaccaatcg ttacccagtt ttttgctccc gttgatgcta ctaagtctgg
     1501 actgtagaaa aacaaaggca ataccataag tcctagccca agtacgtaca agtaaggtgt
     1561 gatttgccac agaaacttag tggaaaagaa catgaccaaa aaagctgcca ctgttccaat
     1621 tcctatccaa gcaatttgtt gaccaaccat ctgtacaaca ttatccggat aatcttgact
     1681 aaccgcaatg tagagagaaa taatccccac aactaataaa aaaaagaccg gtaaaataag
     1741 tgagtagtct actcgactat cgattgttcc cctatttttc atcaatttcc tcatactttc
     1801 acttcaaatt cttactcgtt ccattatagc agaaatcatc tctcattgaa actaaaaatc
     1861 tagttacatc agtggggata cgatttatac tcaacgaaaa tcaaaagtag cctaggaaac
     1921 gaagtcgaag atagaactgg agttcatcca ggcaagttga caacggataa ttttgatttt
     1981 cgaagagtat tactagtgca caaatcataa aatacgtata tcttcatacc ttcgaacagg
     2041 ctgaaatata atgctttacc ttcacacacc ccataagttt gctcaattca tgagtatgta
     2101 gaccaatggc aatcattttg gcaatcacgc acgaaaaagc cctctcttat ttctagagtc
     2161 aaagaaaaaa tggtccactg gaccataact cgctctttta ttttcaagct cgtgaaaaaa
     2221 cagtccgtta aggactgttt ttttatttga gaccgtattt tttgttgaag cgatccacac
     2281 gtccatctgc ttgagtgaac ttttgacgtc cagtatagaa tgggtgtgag tctgatgaaa
     2341 tttccacacg gatcaatggg taagtttcac cttcgaattc aactgtttcg ttagatttct
     2401 tagttgaacc gctaaggaac ttgtagccag tagttgtgtc catgaagaca acagtgcgat
     2461 attctggatg gatatctttt ttcattttaa aattcctttc tgccatggac tctttccgag
     2521 ccatagatta taaccattta agtttaccaa aactttgttc ttatggcaag tctttttacc
     2581 tattttttta atattttttc aagttatagt acagaagcac ctgaagcaag gtcagaagtt
     2641 actctggcgc caaatccccc tcttgaagga ctgcccaagc tatcagaaat tctttcttca
     2701 ccagcaagaa ctttctcaga aagccctgag gtcactagaa tcctgccatc cagcccaatc
     2761 agaaccgctt cgataccatc agtttcttct accatcttat aaatccgagt tatttcttga
     2821 ccaaacagtc gagtcgtcca aatctcacca tcaacagatt gtttggacac aatggtcaaa
     2881 ctagccagct gcgactccat tggatagccg gtctcaggac tcaaaatatg atgataggtc
     2941 tttccattca cggtaagggt tctttcgtaa ataccagagg tgacaaccga ttcttctcct
     3001 attttgagga cgagggcatt ttcaccacgt ggtttctgag gatcctgtat accaattcgc
     3061 caataaccat ctgcattgtg aggagcttga ccaaaggtca ggacgttgcc acccagatta
     3121 atcagcccag ctgccacacc tattcttttc aagaagtcaa caatcttatc tgctacatat
     3181 cctttagcaa gagcacccag atcaatcgcc ataccttctt ttttcaaata gactgtctgc
     3241 tcctcatcat ctaattcgat gtcccttgga ttgatgagct ggagtttttc actgatcatt
     3301 tcttgggttg gaagtttggc atcgctaaat ccaatccgcc aactttgcac aaggggaccg
     3361 atggtaatat tcaaaaaaga tccctcagcc agactgtgtt ctttgcccaa ttttatcaat
     3421 tcatacaggt catcggcaac tggaactgct tgaacaccag cgtttagatt gacctccatc
     3481 aattctgaag tcaaatcatt ggcagaaaac cggtctttat aaagataaag tagctcttcg
     3541 acctgatcta aaattggttc tgcatctgga tgccatattt tcgtttcaat gatggttccc
     3601 attagacgaa gggaacggct acttgcttgc attcctcact acctcttgga tttcttggta
     3661 tatttgctca ttttcggata gtgaataaga gttagcacca cttgctagtg ggtggccacc
     3721 gccatcatgg cgtttggcaa tctcgttgat tggaagatac ttactgcgca aacggactct
     3781 gtaatttccg tcagtttgtt tcacaaaaat cccccaagat tgaacgattc caatccgtcc
     3841 tggagcccca acgataaagg aagtgtccgc atcggtcacc ccgtattttt cgagaatgtc
     3901 ctgtgtcaaa ataacacgcg ccgcaccatg ttcatccact tccaagttgt cgtagacgta
     3961 acctgtcaac ttggcaatct tgaaatcaat ggtatccatc tggcgagaca tccttgcaaa
     4021 gtcaaaatct tcttggcgga gacgagcaac aatctcaaag gtgcgcgagc tggttgccgg
     4081 atagagaaag cggcctgtat cgcccacaat tcctgcatag agtagacgag caatctctcc
     4141 atcgagttcc agttggcatt ggatggctag ttctgtaatc atttcactgc aagaactagc
     4201 ttccgtattg acccagagca ggtcaccata aggctcttca ttggggtgat gatcgatttt
     4261 aatcaagaaa tctccttgag catagcggtc gtcatccaca cgcgccgtat tggcagtgtc
     4321 tgtcacgata accaaagctc cttgataatc ttggtctgca acttcatcca ttcctgccat
     4381 ccaagcaaga tttggttcct caaaaccggt taccttgatg gtttttccag gaaaactctt
     4441 ctgtaagagt ttctgcaagc caacttgact accaatggca tccggatctg gtcgtttatg
     4501 tcggtgaata ataatcgtgt cgtattcgtg aattttttca ataattttac tgtaaattgt
     4561 catagcaact ccttttcatc attttagcat aaacttcccc ttttgaaaat gatgggagtg
     4621 tggtataatg gaattactat gtaatttgga ggaaattatg gcactagcaa aaatagttta
     4681 cgctagtatg actggtaaca cagaagaaat tgctgacatc gtcgccagta agctagaaga
     4741 gttaggtttg gaagtacagg ttcacgaatg tacgactatt gaaaccgaag aaatcttgga
     4801 tgcggacctc atcgtggtag ccagctacac ctattcttac ggtggagacg gtgagcttcc
     4861 agatgaaatt gttgatttct atgctgacct agctgactta gatttgaccg gtaaggtcta
     4921 cggtgtttgt ggttctggcg ataccttcta tgatgacttc tgtagtgcag tagacgactt
     4981 cgatgtcatg ttgggttcac gtggtgcaac taagggtgcc gaaaatgtta aggtagatct
     5041 tgccgctgaa gatgaggata ttgttaacct tgagcaattc gcgacagacc ttgttgctaa
     5101 actagataca atgaattaac tgacaaacag actggcttat caataggtct agtctgtttt
     5161 cttttagcct tttattaaga ttgtacacct attgcttgca atcttaatag ttttgatata
     5221 taattaactg gttaattttt ttgaaaaaag gagaatttta tggctaaaaa atcaaaaatg
     5281 gcacgctatc agagacaatt ggagttaatc gagcgctatg cggatttacg taaaagtctc
     5341 aaagaaaaag gtgactatca agcacttcga aaattgcctc gcgactcaaa tccaaatcga
     5401 ctgaaatacc gcgacaagac cgatggccgt ccgcatgctt atatgcgaaa attcggtgtg
     5461 agtcggatta ccttccgtga attagcccat cttggtcaac ttcctggggt aaaaaaagca
     5521 agttggtaac ttcctcaaac aaggctagta ttctgccttg ttttttgcta tactaaaaaa
     5581 caactgaaac aatatacgga ggtttataag atgaatttgg attgcatccg cgaacagatt
     5641 aacacgattg atagccaatt ggttgaacta cttgaaaaac gaatggagtt ggtcgaccaa
     5701 gttacagcct ataaacgagc aacaggtaag cctgttttgg ataccagccg agaaaatgct
     5761 gtccttgaaa gagtcggaaa attagttcaa aaggatgatt accgctctgc cattcaagca
     5821 acctttagcg atattatggc ccaatcaaga acctatcaat cttccaaatt agcgaatcat
     5881 gagcaataag atgaaacatg ccatccaact ccttttcttt tccagcttaa ttggagtcgg
     5941 tgcaggaata atcaccacac tttttgggaa aattctacta gggattggag acttacggtc
     6001 tgaatatttt acatatctaa tcccctttct gccacttgca ggtgtgttga ttgttttcat
     6061 ttatcaaaaa tgggggaggg aagtccaagc aggtatgggc ttagtcttca aagctggaca
     6121 aggggagaaa gcgcaaattt ctccagttct cattccactc atcatctcta caacttggct
     6181 cagtcacctt gtcggtgctt ccgttggtcg cgaaggtgta gctgtccaac ttggagccag
     6241 tctctcacac tggttacaaa aacatggttt cacacacttg cccaaagata tgataacaaa
     6301 gattggtatg gccgcaggtt ttgcagggtt atttcaaaca ccatgggctg ctggtttctt
     6361 tgccattgaa gttttaatcg ttggccaata ttcttggact agtctcccct attgtctagt
     6421 tgccgctttc acggcttcta ctacttccca tttactggga ttggagaaat tttcgcatac
     6481 catcgctact acctcattct attttacaga tagttttaaa tggctattta ttgccctttg
     6541 cttcggtttc atcggcaatt tatttgcttt gtttctggcg caagcaaaaa gcatctcaac
     6601 tcgatggctt ccaaatcctt acattaaaat agctatcatg ggagttggac tgaccgttct
     6661 actatttttc ttccatcaag gtcgttatac tggtctagga accaatttga tcgatgccag
     6721 tttagctggg gaacaggtgt ttgctttcga ttggctacta aaactcctac taacctgtct
     6781 ttgtttggct gcaggttttc aagggggcga agttactccg ctctttgcga ttggggcaag
     6841 ttctggggct gttttagcag gattactagg tctaccaact gaacttgttg cagcccttgg
     6901 gtattgtgcc gtatttggaa cagccaccaa taccttactg gctcctctct tcattagtta
     6961 tgaagtcttc ggcgctaata tcctgcccta tgctattcct gtacttgcca tagcttatct
     7021 tataaacaga aaacagacta tttatggaca gcagttgagg aaattcaata atgcaaaaaa
     7081 gcctatcatc taggcttttt cgtatactct tcatctcccc tgcaggaatc gaacctgcaa
     7141 ctaattctta ggaggaattt gttatatcca tttaactaag ggaagtctgc ttctctattg
     7201 taccccagaa gagagcagat tgcaagagca aggttatata agttttttca aatctttaca
     7261 aaaagcagaa cctactctaa gatgaaacac aattgttttt atgtatttct ctcataatag
     7321 tctctaaaag aagtaattct aaaatgtact tcgaaagttt gtcgttttta tgcttgattc
     7381 ctactcccct tccttacgtc tcactgagac agccattccg agtgaaacta agccacccaa
     7441 gctaataaga agtgagccta gggcttcttg accggtattt ggaagtgttt gatttcctga
     7501 tgctttttca gttttcttat ttgaaatagg agccatactt ccagatggtg ccggttgctc
     7561 ggttgctttc tgatttgtta cactatcgcc accgtcattt gctttctcag gtgttttcac
     7621 atcagtagtt gtcccagcat tatcttcttt ttcttctgct tttggttctt ctacgtactt
     7681 ctctacaaat gcttttctac ctggaattgt tgcactaatg gtttgacctg ctttttctaa
     7741 atcagtcaag tactccacaa atacttctgt atctggattg atagcaccaa tcagtttagc
     7801 ttctttgaaa atcgagaagc catccccacc accaaataag aagtcgttga tgacaagtgt
     7861 ataggtttct gtcggaacaa tctccgtccc atcttctttg aaggctttaa caaccttata
     7921 aggattttct tccgttggat tgtcagcctt cgtgtagata tatttaattc cagacatttg
     7981 aaggaaatat ttttcacctt catcatattg ttgatttaag gctgtataaa tctgctcacc
     8041 tgtcatttga acgacttgta ggatattccc aaatggttga acagcttgtg ctgctcccca
     8101 agtaactgtt ccatcttctt ggaccttcaa atctgcccga atcccgccat cgtttgtcat
     8161 tgcaaagtca acatcataac ccgatttctt agcgatagct aattgagccg atgttactag
     8221 attacctaca gcactttctt taaattcatt cacttcgcgt gaaatatctg tcgcttgact
     8281 agctgtagca attttttgct cagttacttt tttaacaatg gtatttgcct catctacaat
     8341 tgcctgaatt tccggacttg gtgttttctg ccctggtgct actgcaataa ttttcgcagt
     8401 cggaacagct ttaaagtcgg caatatctgt atcataaaca gccctaacat ctgcgtaagc
     8461 cttaccttgt gaggtagctt gaacaatcaa ggttttgccc gttgtaccgt ttgtatagac
     8521 atggttatgg ccagcaaata caaggtcaac tgagtgttca ggatagattt catttagctt
     8581 agcaatcata tctgcagctt caccagcagc cacaccatcc ttgcttgtag ccggaacgtg
     8641 agccagtaca actatcgcat ttacaccttt ttcagctaat tcacgcgcat atttagcaat
     8701 cgtctctgcc tcattcaaaa aagtgtactg ctcatagttt ttcttcaaaa caagattagg
     8761 aatttctgtc gtaactacac caataaagcc aatcttagct tctttatcat tcacgggaat
     8821 agtcttaata gcgtagggct tccaaccata cggaatttca cctgtttctt tgtcaataac
     8881 gttagcaata acaatctcct gtttagcagc ttcacgagta taattatcta caatctcatt
     8941 aaactgacct ttttttggag cttcaccagt catgatacgg ttatactcat caagtccctc
     9001 atcaaactca tggttcccca aagttccgta ttcaacatcc attttattaa agacttttac
     9061 agttggttca tcttgcaaaa gtccagaatt cgatggactt gcaccaacca tatccccagc
     9121 ttgaacacgg atagactctg caggtgtctc tgtttctgct gctgtttctt caaattctgc
     9181 ttgtgaatca tccatgtaag catcaagtaa agcggcagtt cctgcattac gaactgtttc
     9241 cccttccaat cgcgctgtcc ccgtcatatc aagcgcacca tggaaatcat taactcccat
     9301 aatttggaca gctaattcat ctgctaaaac agcttgtgtt gcaataacgc taaaaccagc
     9361 tacaagagct agtatactgc ttttcaaccg aatattcttt ttcataagca aaacaatgct
     9421 ccttctattt ttttcatatt gacatatcta taataatcct tttcttacg
//
LOCUS       DBNFCK010000068         9360 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.068, whole genome shotgun sequence.
ACCESSION   DBNFCK010000068 DBNFCK010000000
VERSION     DBNFCK010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9360
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.068"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            2..523
                     /locus_tag="AC1PIW_001308"
     CDS             2..523
                     /locus_tag="AC1PIW_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6606242.1"
                     /translation="MKRPIHLYIFVILSSIASILRLFSAFVSTFNEERLRTSVQGVVG
                     IDVEELILVSRETANLQTGIIQKTAALVMFGLLIAVIVFLFMKKNELASYLYIGYLFS
                     TLLLNTYSYLAGKGIANLYSDAALRDVTAAGMLGAYILNIVLFAIYFGVTVFFHLRKP
                     KEKPSTAINSTDI"
     gene            complement(576..1490)
                     /locus_tag="AC1PIW_001309"
     CDS             complement(576..1490)
                     /locus_tag="AC1PIW_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIP6606243.1"
                     /translation="MKTFLRRKGIACQLLAAIFIVGINLQSPPSASANARGDDYPYTA
                     VDAVDPWRLYTRQCTSFVAFRLSTVNGFTLPPAYGDANVWGHRAKQEGYQVNMTPAVG
                     AVAWWETPMHVAWVSAVNGDTVEIEEYNYGVRYTYGRRVIPKNAVSGYIHFKNLGAGT
                     TNSQPALAPSGTYTFTSQLAIKSQASLSSPTLDYYYAGESVRYDKVLEDDGQRWISYI
                     SYSGARRYIAIQPTITQSTNNSLPASGSYTIQSYSSVRNNPSLSSPEITFYGAGSVVR
                     YDKVITAEGRTWISYISYSGARRYIAIS"
     gene            complement(1670..3358)
                     /gene="argS"
                     /locus_tag="AC1PIW_001310"
     CDS             complement(1670..3358)
                     /gene="argS"
                     /locus_tag="AC1PIW_001310"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HIP6606244.1"
                     /translation="MNQKQVIAERLAAILPSLEVEAIYNLLEKPKSSEMGDIAFPAFS
                     LAKVERKAPQAIAADIVEKLDTTGFENVVATGPYVNFFLDKAAISHQVLTDVITEKDQ
                     YGKLNIGQGRNVTIDMSSPNIAKPFSVGHLRSTVIGDALANIHEKLGYKPIRINHLGD
                     WGKQFGMLIVAYKLWGDKAAVEADPISELLKLYVRINAEAEEKPELDDEARQWFKKLE
                     DGDPEAHELWQWFRDESLVEFNRIYDKLDVTFDSYNGEAFYNDKMDEGIQILEEKGLL
                     QESKGAKIVDLESYNLPPALIMKTDGATLYITRDMATAMYRKRTYDFVKSIYVVGQEQ
                     INHFKQLKAVLKEMDFDWSDDMTHITFGLVTKDKKKLSTRKGNIILLEPTLDEAISRA
                     LTQIEAKNPDLENKEEVAHAVGVGAVKFYDLKTDRDNGYDFDLEAMVSFEGETGPYVQ
                     YAYARIQSILRKANFVPNAENDYKLADTESWEIIKHIQNFSAVVERAGDKFDPSLIAK
                     YAINLAQAFNKYYAHTRILDESPERDSRLALAYATGLVLKEALRLLGVKAPEKM"
     gene            3465..3905
                     /gene="argR"
                     /locus_tag="AC1PIW_001311"
     CDS             3465..3905
                     /gene="argR"
                     /locus_tag="AC1PIW_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HIP6606245.1"
                     /translation="MNKAGRHDLIKAMIRQEKIGRQTDIQQGLEARGVVVTQTTLSRD
                     LRELGVIKIHENGRSFYSLAIEEERVNFIQLLAQYAYKVDRASFILVLHSELGEAALM
                     ANIIDAEKPETILGTLAGANTLLVICRDDVAAQQVEAEINYYLN"
     gene            3920..6460
                     /gene="mutS"
                     /locus_tag="AC1PIW_001312"
     CDS             3920..6460
                     /gene="mutS"
                     /locus_tag="AC1PIW_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HIP6606246.1"
                     /translation="MATEKISPGMQQYLDIKAQYPDAFLLFRMGDFYELFYEDAVEAA
                     QILELSLTSRNKNAENPIPMAGVPYHAAQQYIDTLVELGHKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVTDSSKMGADSNYLVAIDRQGVQFALSYMDVSTGQFFVTSLDDFTS
                     LCGEIRNLRARELVIGYALSEEEEQVFSNQMNLLLSFEDEVTEDVQLIDNSLTDLEKA
                     AAGKLLSYLHRTQMRDLSHLQKVVHYEIKDYLQMDYATKSSLDLLENGRTGKKHGSLY
                     WLLDETKTAMGMRLLRTWIDRPLIDLKRIENRQAVVQVFLDYFFERSDLVEALKGVYD
                     IERLASRVSFGKTMPKDLLQLSQTLGNIPAIKNILQQINEPALGNLVAGLDPIPELHA
                     LISSAIDPEAQGTITDGNIIRTGFDETLDQYRLVMREGAGWIAEIEAKEREASGINNL
                     KIDYNKKDGYYFHVTNSNLGNVPDHFFRKATLKNSERYGTEELAKIEGQMLEARDKSA
                     NLEYEIFMRIRQEVEKYIGRLQKLARTIATIDVLQAFAVVAEQQHLVCPRFTDQRELT
                     IDRGRHAVVEKVMGKQTYIPNSIHLNTDTHMQLITGPNMSGKSTYMRQLAVIVIMAQM
                     GSYVPADQAELPIFDAIFTRIGAADDLVSGQSTFMVEMMEANKAVRLATDRSLILFDE
                     LGRGTATYDGMALAQSIIEYIHDKIGAKTLFATHYHELTDLSQTLEHLENVHVSTLEK
                     DGQVTFLHKIAQGPADKSYGIHVAKIAGMPEELLQRADRILQTLENQAPTAPTHPAPS
                     VVEEPSGQLDLFADTPSHPVLDELEKLDIYNMTPMEVMMAVAELKKKI"
     gene            6592..7035
                     /locus_tag="AC1PIW_001313"
     CDS             6592..7035
                     /locus_tag="AC1PIW_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HIP6606247.1"
                     /translation="MKVKLAISSEILEDLVTIEAQAMSEQITNLVTYIQNLDKQRSSL
                     TVKKGEQVYLVEHDEIVRLYLEDRVLQVETVETTYTSNLRLYQVKEDLPANFLQISQS
                     EIIHIKQLDHLKLTANGLVKLVMKNGSVTYSSRRYLKVIKERLGL"
     gene            7032..7472
                     /locus_tag="AC1PIW_001314"
     CDS             7032..7472
                     /locus_tag="AC1PIW_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HIP6606248.1"
                     /translation="MKKYILSASLGIAIGTIISIITSAVFGQGTYLPLNPFSTMGAYY
                     LSNFSQVTVMLICVAIWATIGILFQLADKIFEQDWSLLRMTATHFGFTVLGFTPLGIL
                     AGWFPVKLGALLFFWLIFVLVYALLFFLNYRKMERQIKDINGRL"
     gene            7506..7694
                     /locus_tag="AC1PIW_001315"
     CDS             7506..7694
                     /locus_tag="AC1PIW_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606249.1"
                     /translation="MKIILTIVYSLGVVWNTLYIAYNVGKLPLLNLLWPAVILVACAI
                     NLYFLWTRKQESESMSLS"
     gene            complement(7809..9098)
                     /locus_tag="AC1PIW_001316"
     CDS             complement(7809..9098)
                     /locus_tag="AC1PIW_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HIP6606250.1"
                     /translation="MLDQIKAHLLDSINDIVSIANQFVLHPEKDFSRKSQLTMETMIQ
                     AILTMGGNTLAKELLDLDLPVSQSAFVQRRYQLKHQAFKALFANITSKIPTFKDLPIL
                     AVDGSDVVLPRNRSDKTTTFQTGPHHTPYTLIHINALYNLEQEIYHDLRIQNNREVDE
                     RAAFIDMMESCPFEQALVIMDRGYESYNVMAHCQERNWSYIIRIRDGNHSMKSGFNLP
                     DTPCFDEEFDLNICHKQTNVMKELYRDFPNQYHFLPHNASFDLLPNSSRKSDPISFYD
                     LHFRMVRLEIKPGFFETLVTNTDYSPEKLKDLYAYRWGIETSFRDLKYSIGLTHFHAK
                     KKEGILQEIYAHFINFNVCKWLTSHVAIKPSKLKQAYKICFSDAVYACRKFLREELTS
                     FQLETYIAKHLSIILPNRTFQRKIKSKAPVSFTYRIS"
BASE COUNT         2525 a         1842 c         2259 g         2734 t
ORIGIN      
        1 tatgaaaaga ccgattcatt tgtatatttt tgtgatacta tccagtattg ccagtatttt
       61 aaggctgttt agtgcctttg tgtcgacttt taatgaggag cgacttcgta cgtctgtgca
      121 gggagttgtt gggattgatg tagaagaatt gattcttgtt tcgagagaaa cagccaatct
      181 ccagacgggt atcattcaaa agacagcagc cctggtcatg tttggtcttt tgattgcggt
      241 cattgtcttc ttattcatga agaaaaatga gctggcttct tatctttaca tcggctatct
      301 attttcgacc ttactgctga atacctatag ctacctagca ggtaagggga ttgccaacct
      361 ctactcagat gcagccttgc gtgatgtgac agcagcaggt atgctagggg cttacatcct
      421 taacattgtc ctatttgcca tttactttgg tgtgaccgtc ttcttccatc tgcgtaaacc
      481 gaaagaaaaa ccgagcacag ctattaattc gacagatatt taatgaaaaa gcacagtcta
      541 aaagattgtg cttttaaata ttgtattttt tgtgattagg agatggcgat gtagcgtcga
      601 gcacctgagt aactgatata gctaatccat gtgcgacctt cggcagtaat aactttatca
      661 tagcgaacga ctgagccagc tccgtagaag gtgatttcag gactggacag acttggattg
      721 ttgcggacac tagagtagga ctggatggtg tagctacctg atgctggtag gctgttgttt
      781 gtagattgag taatggtagg ctggatggcg atatagcgcc gagctccaga atagctgata
      841 tagctaatcc agcgttgtcc gtcgtcttca agaaccttgt cgtagcggac gctttcgcca
      901 gcataatagt aatcgagggt agggctagat agactagctt gagatttgat ggccagttgg
      961 ctggtaaagg tataggtgcc gcttggggct aaggcgggtt gagagttggt tgttcccgct
     1021 ccgaggtttt taaaatgaat gtagccgcta acggcatttt tggggataac tcgccttccg
     1081 taggtatagc gaacaccgta gttgtattcc tcaatctcta cggtatctcc atttactgca
     1141 gaaacccatg cgacatgcat gggagtttcc caccaggcaa cggctccgac agcaggtgtc
     1201 atattaactt gataaccttc ttgtttggca cgatggcccc aaacattggc atcaccatag
     1261 gcaggtggca atgtaaaacc gttaactgtg ctaagtcgaa aggctacaaa ggaggtgcac
     1321 tggcgtgtgt agagtctcca tggatcgact gcatcaactg ctgtgtaggg atagtcatct
     1381 cctcttgcat tggctgaggc tgaaggtggg gattgtaaat tgataccgac aataaaaatg
     1441 gcggctagaa gctgacaggc gattcctttt cttctcaaga atgttttcat tatttctcct
     1501 tgtgtagtag atgtagaggt atcacgtgta cctcccatct atatattcgt aattgaaatg
     1561 aatctttttg attttcattt gggagaagga gttgttgtga taaggaatcg gaaacgagag
     1621 taggcaatgt ggtagaaaaa aagcctcggc tgatggccga aggctttgat tacatttttt
     1681 ctggtgcttt tacgccgaga agacgcagag cttctttgag gacaagtcct gttgcgtagg
     1741 caagtgccaa gcggctgtca cgctctggac tttcatccaa gatacgcgtg tgggcgtagt
     1801 atttgttgaa ggcttgagct aggttgatag cgtatttggc aataagtgat gggtcaaatt
     1861 tgtctccggc acgttctaca acagctgaga agttttggat gtgcttgatg atttcccagc
     1921 tttctgtgtc agccagtttg tagtcatttt ctgcgtttgg tacaaagttt gccttgcgta
     1981 ggatagactg gatgcgggcg tatgcgtatt gtacataagg acctgtttca ccctcgaagg
     2041 aaaccatagc ttccaagtcg aagtcgtagc cgttgtcacg gtcggttttg aggtcgtaga
     2101 acttcacagc gccgacacca actgcgtgtg ccacttcttc cttgttttca aggtcagggt
     2161 ttttagcttc aatttgagta agggcgcgtg aaatagcttc gtctagagtt ggctcgagca
     2221 ggatgatgtt tcctttacga gtagagagct ttttcttgtc cttggtcact agaccgaagg
     2281 tgatatgggt catatcgtcg ctccagtcga aatccatttc cttcaaaacg gctttgagtt
     2341 gcttgaagtg gttgatttgc tcctgaccaa cgacatagat gcttttcacg aagtcatagg
     2401 tgcgcttgcg gtacatagct gttgccatat cgcgcgtgat gtagagggta gcgccatctg
     2461 tcttcatgat aagggctggt ggaagattgt agctttcaag gtcaacgatt ttggcacctt
     2521 tagactcttg aagaagtcct ttttcttcta agatttggat accttcgtcc atcttatcgt
     2581 tgtagaaggc ttcgccgtta tagctgtcga atgttacatc aagtttgtcg tagatgcggt
     2641 taaattcgac caagctctca tcacggaacc attgccacaa ttcatgcgct tctgggtcgc
     2701 cgtcttccaa ttttttgaac cattggcgtg cttcgtcgtc caactcaggt ttttcttctg
     2761 cctcagcatt gatacggaca tagagtttaa gaagttctga gattgggtca gcttcgaccg
     2821 cagccttgtc accccagagt ttgtaggcaa caatcaacat gccaaattgt ttgccccagt
     2881 cacccaagtg gttgatacgg attggcttgt agcctagttt ttcgtggatg ttggcaaggg
     2941 catcgccgat aacggttgag cgtaaatgtc caactgagaa tggtttagca atgtttggac
     3001 tagacatgtc gatggttaca ttgcgtcctt gaccaatgtt gagtttaccg tattggtctt
     3061 tttcagtaat aacatctgtc aagacttggt gggaaatcgc agccttgtcc aagaagaagt
     3121 tgacgtaagg accagttgca acaacgttct caaagccagt tgtatcgagt ttttcaacga
     3181 tgtccgctgc gatagcctgt ggagccttgc gttccacttt agcaagtgag aaggctggga
     3241 aggcaatatc gcccatttct gatgattttg gtttttctag cagattgtag atagcttcca
     3301 cttccaaact cggaaggatg gcagccaatc tttctgcaat cacttgtttt tgattcatat
     3361 aggatttcct ccaaagttat tgattctatt ttaacacatt tctaggctaa tagcggggaa
     3421 aagtgttata atatttctat aaatatacat tttgaggtaa aatgatgaat aaagcaggac
     3481 gacatgattt aataaaggcc atgattcgtc aagaaaaaat agggcgacaa acggatattc
     3541 agcaaggttt agaggctcga ggggtagtgg taacccagac aaccttgtct cgtgatttac
     3601 gtgagttggg agtgataaaa attcatgaaa acgggcgttc attttattcc ttagctatcg
     3661 aagaagagcg cgtgaatttt atccaattat tggcacaata tgcgtataaa gtagataggg
     3721 ctagttttat tctggtcctt cattcagaac tgggagaagc ggccttgatg gccaatatca
     3781 ttgatgcaga aaagccagag actattttgg gaacgctggc gggagctaat acacttttag
     3841 ttatttgtcg agatgatgta gcggctcagc aagttgaagc ggagattaac tactatttga
     3901 attagaacaa ggagagatga tggcaacaga aaaaatttct ccgggaatgc agcagtatct
     3961 ggatataaaa gcacagtatc cagatgcttt tttgcttttc cgtatgggag acttttatga
     4021 attattttat gaagatgcgg tagaagcggc acagattttg gaattgtcct tgaccagtcg
     4081 gaataagaat gcggaaaatc cgattcccat ggcgggggta ccgtatcacg cggcacaaca
     4141 gtatatcgac acacttgttg aattggggca caaggtcgca attgccgagc agatggaaga
     4201 tcctaagcag gcagtcggcg tggtcaagcg ggaagtagtg caggtcatta ctcctggtac
     4261 ggtaacagac tcgtccaaaa tgggagcaga cagtaactat ttggttgcga ttgaccgtca
     4321 gggagtacag ttcgcgcttt cttatatgga tgtgtcaaca ggtcagtttt ttgtgactag
     4381 tttggatgat tttactagtc tttgcggtga gatccgcaat ttacgggcac gtgaattggt
     4441 catcggctat gctttatctg aggaagaaga gcaggtcttt tcgaaccaga tgaacctcct
     4501 gctctctttt gaggatgagg tgacggaaga tgtccagctg attgacaact ccttgacaga
     4561 cttggaaaag gctgcggcag gtaaactcct cagctaccta catcggactc agatgcgaga
     4621 tctgagccac ttgcaaaagg tggtccacta tgaaatcaag gactatctgc aaatggacta
     4681 tgcaaccaag tccagtctgg acctgcttga aaacggtcgg acaggcaaga agcatggtag
     4741 tttgtactgg ttgctagacg aaactaagac agccatgggt atgcgactat tgcggacatg
     4801 gattgatcgt cccttgattg atcttaagcg gattgagaat cgtcaggctg tcgttcaggt
     4861 ttttctggat tacttctttg aacggagtga cttggtcgag gctttaaaag gtgtctatga
     4921 catcgaacgt ttggccagcc gggtgtcttt tggaaaaacc atgcccaagg atctcttgca
     4981 gctctcccag actttgggaa atatcccagc aatcaagaat atcttgcaac aaatcaatga
     5041 gcctgctcta ggcaatttgg tggctggatt ggacccaatc ccagaactgc acgctctcat
     5101 cagctctgct attgacccag aagcccaagg gaccatcaca gatggaaaca tcatccgtac
     5161 aggctttgac gaaacgctgg atcagtatcg tctagtcatg cgtgagggag cgggttggat
     5221 tgcggagatt gaggccaagg agcgtgaagc atctggtatc aataatctta agattgatta
     5281 caataaaaaa gacggttact atttccatgt taccaattcc aatctgggca atgtgccgga
     5341 ccattttttc cgtaaggcga ccttgaaaaa ctcggagcgc tatggaacgg aagagttggc
     5401 taagattgaa gggcagatgt tggaggcgcg tgataagtct gctaatttgg agtacgagat
     5461 tttcatgcgg attcgccaag aggttgagaa gtatataggt cgcttgcaga agctggctcg
     5521 gaccattgca accatcgatg ttttgcaggc ctttgcagtt gtggcggagc agcaacattt
     5581 ggtttgtcca cgctttacag atcaaagaga actgaccatt gaccgtggtc gtcatgcggt
     5641 ggtggagaag gtcatgggca agcagaccta cattcccaac tctattcact tgaataccga
     5701 tacacatatg caactgatta ctggtccaaa catgagtggt aagtctacct atatgcgaca
     5761 gttggcggtt atcgtcatca tggcgcagat gggttcctat gtgcctgcgg atcaggctga
     5821 attaccgatt ttcgatgcta ttttcacccg aattggggcg gcagacgact tggttagtgg
     5881 tcaatcgacc ttcatggtcg aaatgatgga ggccaataag gcggtccgtc tggcgacaga
     5941 tcggtcgctc atcctctttg atgagttggg acggggaacg gccacctatg acggtatggc
     6001 cctggcccag tctatcatcg agtacatcca tgacaagatt ggtgccaaga ctctctttgc
     6061 cacccactac catgagttga cagaccttag ccagaccttg gaacacttag agaatgtcca
     6121 tgtatcgacc ttggagaagg acggccaagt caccttcctc cacaagattg cccaaggtcc
     6181 agctgacaag tcctacggga ttcatgtggc taagattgcg ggaatgccag aggaactatt
     6241 gcagcgggca gatcggattt tgcagacgct tgaaaaccaa gcccctactg caccaactca
     6301 cccagctccg tcagtagtgg aagaaccgtc aggtcaactt gatctcttcg cagacacgcc
     6361 ttctcaccca gtacttgatg aactggaaaa actagacatc tacaatatga cccctatgga
     6421 agtcatgatg gcggtggcgg agttgaagaa aaaaatataa gacaatgaaa gtcaggacag
     6481 ttgttctggc ttttttgccg tttagtgctg gaaaatgacc acatagccaa aagcccttga
     6541 aaccagtaaa gaaatggtgt ttaatagggt cagaaagaga aaggagagaa tatgaaagta
     6601 aaactagcca tatcatcaga gattttggaa gatttggtga ctattgaagc tcaagctatg
     6661 tctgagcaaa ttactaactt ggtgacctat attcaaaact tggataagca gagatccagt
     6721 ctaactgtta aaaaagggga gcaggtctat cttgtggaac atgatgagat tgtcagactc
     6781 tacttggaag atagggtttt acaggtggaa acagtagaaa ctacctatac ttccaaccta
     6841 cgcttgtatc aggttaagga ggacttgccg gcaaactttt tacaaatctc tcagtcggaa
     6901 atcattcaca tcaagcaact ggaccacctc aaactgactg ctaacggtct ggtcaaactg
     6961 gtcatgaaga atggctcggt cacctactcc tcccgtcgct atctaaaagt tatcaaagaa
     7021 aggttaggac tatgaaaaaa tacatcttat cagccagttt aggcatcgcc atcggaacca
     7081 ttatctccat tatcacatca gccgtcttcg gacagggaac ctaccttcct ctaaatccct
     7141 tttcaaccat gggtgcttac tacctgagca actttagtca agtaaccgtc atgttgattt
     7201 gtgtagccat ttgggcgaca atcggtatct tgtttcagct ggcagacaag atttttgaac
     7261 aggattggag ccttttacga atgacggcaa cgcattttgg tttcacggtt ttaggcttta
     7321 caccgctggg cattttggct ggctggtttc ccgtgaaact tggggccctt ctctttttct
     7381 ggctcatctt tgtcttggta tacgctctgc tatttttcct aaactatcgg aaaatggaac
     7441 ggcagattaa ggacatcaat ggtcgtttgt agtataatag cactatcatt taattggagt
     7501 gtcttatgaa aattattttg acgattgtct atagcttggg ggtagtgtgg aatactctct
     7561 atattgccta caatgtcggt aaacttcctt tgttaaattt gctttggcct gctgtgattt
     7621 tagtggcctg tgccatcaat ttgtactttt tatggaccag aaaacaagag tcagaatcaa
     7681 tgtcattaag ttaagcattt aagcagctaa agaaactatt cccgtctgag caattgatga
     7741 caaaacggga atagtttttg tatttagggc gcacaaaaag gaagattttt aacccaattt
     7801 tttaactatt atgatattct ataagtaaag cttacaggtg ccttgctttt tatctttctt
     7861 tggaaggttc gattgggtag aataatggat aaatgcttag caatgtaggt ttctaattgg
     7921 aaggaagtga gttcttctct aagaaatttt cgacaggcat aaacagcgtc tgaaaaacaa
     7981 attttataag cctgttttaa ctttgatggt ttaatagcaa cgtgtgaggt tagccattta
     8041 caaacattaa aattgataaa gtgagcgtag atttcttgga gaatcccttc cttctttttt
     8101 gcatgaaaat gagtcagacc gatactgtat tttaggtcac gaaaactagt ctctatgccc
     8161 catctgtagg catagagatc ttttaatttt tctggtgaat aatccgtatt tgtcaccaaa
     8221 gtctcaaaga aacctggctt gatttcgaga cgcaccatcc gaaaatgaag gtcataaaaa
     8281 ctgattggat cactttttcg actagagttt ggtaaaaggt caaaggaagc attatgaggt
     8341 aaaaaatgat actgattagg gaagtctcga tacagttctt tcatgacatt ggtttgtttg
     8401 tgacagatgt ttaggtcaaa ctcttcatca aaacaagggg tgtcagggag gttaaatcct
     8461 gatttcatag aatgattccc gtcacgaata cgaataatat aggaccaatt tctttcttgg
     8521 cagtgagcca tgacattgta ggattcatac cccctatcca ttatcaccag agcttgttca
     8581 aaagggcaac tctccatcat gtcgatgaaa gccgcacgct catcaacctc ccgattattc
     8641 tgaattcgaa ggtcatgata aatctcttgt tcaagattgt aaagagcatt gatatgaatg
     8701 agagtgtaag gagtgtggtg tggtccagtt tggaacgtgg ttgttttatc agaacgattt
     8761 cttggtagaa ccacatcact accatcaaca gccaagatag ggagatcttt aaatgttgga
     8821 atcttagaag taatattggc aaacagggct ttaaaagctt gatgtttaag ctgataccgt
     8881 cgttggacaa aggcagattg agagacgggc aaatctaaat caagtagctc tttggctaag
     8941 gtattaccac ccatggtcag tatagcttga atcatggttt ccatagttag ctgacttttc
     9001 cgactaaaat ctttttcagg atgaagcaca aactggtttg cgatggaaac gatgtcgtta
     9061 atactatcaa gtaagtgagc tttaatctga tctaacatga cttttcccct tttattttta
     9121 gtgtaccata gaaaaaacta acctaccaca aaacgtgata ggttagttaa cttaatgaca
     9181 ttggtgggtg acccgcccta gcctattttc aatacataga gccagccctg ttagagggct
     9241 gttttttagg ttttctgata gagaagttag acctttgccc aatctgtttt catatttcaa
     9301 tctagtaatt catccttgtc gagtggtgaa attgggcaca gttgttttcc aattgataga
//
LOCUS       DBNFCK010000069         9280 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.069, whole genome shotgun sequence.
ACCESSION   DBNFCK010000069 DBNFCK010000000
VERSION     DBNFCK010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9280
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.069"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(50..280)
                     /locus_tag="AC1PIW_001317"
     CDS             complement(50..280)
                     /locus_tag="AC1PIW_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1797 family protein"
                     /protein_id="HIP6606251.1"
                     /translation="MESHLVRIINRLEAMANDGGTLKRNFERDGIVVAEVAYSYDEEN
                     GSVFTLRDVAARETYAFDSIDLIAMEIYELLY"
     gene            504..2732
                     /locus_tag="AC1PIW_001318"
     CDS             504..2732
                     /locus_tag="AC1PIW_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIP6606252.1"
                     /translation="MLCKNCNINDATIHLYTNLNGKQQQVDLCHNCYQIMKTDPHNAI
                     LRGLGDLTNPNNMDPFSEFFNHLGGYPGNTPAGKNREQTPPTQAGGHNGRGGQTPPPQ
                     QPQQPNGLLEEFGINVTEITRRGDIDPVIGRDQEITRVIEILNRRTKNNPVLIGEPGV
                     GKTAVVEGLAQKIVDGDVPQKLRDKEVIRLDVVSLVQGTGIRGQFEERMQKLMEEIRN
                     RREIILFIDEIHEIVGAGSAGDGNMDAGNILKPALARGEMQLVGATTLNEYRIIEKDA
                     ALERRMQPVKVEEPSVEETITILKGIQNKYQDYHHVKYSDAAIEAAAVLSNRYIQDRF
                     LPDKAIDLLDEAGSKMNLTLNFIDPKEIDQRLIDSENRKAQATRDEDYEKAAYFRDQI
                     AKYKEMQKATISEEDIPLITEKEIEAIVEQKTNIPVGDLKEKEQSQLVSLASDLKAHV
                     IGQDEAVDKIAKAIRRNRVGLGAPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSADS
                     MIRFDMSEYMEKHAVAKLVGAPPGYVGYEEAGQLTEKVRRNPYSLILLDEVEKAHPDV
                     MHMFLQVLDDGRLTDGQGRTVSFKDTIIIMTSNAGTGKVEASVGFGAAMEGRTQSVLG
                     QLSNFFTPEFMNRFDGIIEFQPLSKENLLEIVSLMLDDVNKRLSHNGISLHVTDKVKE
                     KLVDLGYNPKMGARPLRRTIQDQIEDAITDFYLEHPAEKDLRAVMSSKGTIQIKAQTK
                     TK"
     gene            2918..3379
                     /locus_tag="AC1PIW_001319"
     CDS             2918..3379
                     /locus_tag="AC1PIW_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIP6606253.1"
                     /translation="MEKIAVFGEKKAGVSYQSRFGVYAVIPDEKKEQIILVQAPNGAW
                     FLPGGEIEKGENHLIALERELMEELGFTAEIGKYFGQADEYFYSSHRDTHFYNPAYIY
                     EVTSYHQIGQPLEDFNHIAWFPVNEAIDKLKRGSHKWGIEQWKLQENSFNN"
     gene            3434..3742
                     /locus_tag="AC1PIW_001320"
     CDS             3434..3742
                     /locus_tag="AC1PIW_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HIP6606254.1"
                     /translation="MKIINTTNSHPNLVQSQLANTDAFLVETYSAGNTDVIFTQAPRH
                     YELLISNKYRAVQQNEIEQIRDFFLHRKIDERTINKAAIQTIHTDRLIEMSIPIIAEV
                     "
     gene            complement(4121..6910)
                     /gene="ileS"
                     /locus_tag="AC1PIW_001321"
     CDS             complement(4121..6910)
                     /gene="ileS"
                     /locus_tag="AC1PIW_001321"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HIP6606255.1"
                     /translation="MKLKETLNLGKTAFPMRAGLPTREPEWQKAWDEANLYARRQELN
                     EGKPAFHLHDGPPYANGNIHVGHALNKISKDIIVRSKSMSGFRAPFVPGWDTHGLPIE
                     QVLAKQGVKRKEMDLVEYLEMCRDYAFSQVDKQRDDFKRLGVSADWENPYITLTKDYE
                     AAQIRVFGAMADKGYIYRGAKPVYWSWSSESALAEAEIEYHDIDSTSLYYANKVKDGK
                     GLLDTDSYIVVWTTTPFTVTASRGLTMGADIDYVLVQPAGSDRKYVLAEALVDSLAAK
                     FGWESFEVISKHKGAEFEYIVTEHPWDREVDELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGVKYDLEVAVTVNERGLMNEAAGPDFEGQFYDKVVPTVKEKLGDLLLASEVINH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDQIEATTFNPSWGKTRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTKEVTDHVAALFEEHGSIIWWKSDAKDLLPAGF
                     THPGSPNGEFTKETDIMDVWFDSGSSWNGVMNARENLAYPADLYLEGSDQYRGWFNSS
                     LITSVAVNGHAPYKAILSQGFVLDGNGMKMSKSLGNTILPSDVEKQFGAEILRLWVTS
                     VDTSNDVRVSMDILGQVSETYRKIRNTLRFLIANTSDFNPASDAVAYEELRSVDQYLL
                     VKFNKLVVQIREAYDNYDFMGIYKSVVNFVTLDLSAFYLDFAKDVVYIDGAKSLSRRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEHEAEEFVQLAEMPEVETFANEAQLLA
                     DWENFMVFRTQAQKALEEARNAKVIGKSLEAHLTAYVSAETKSFLESLNADLAQLLIV
                     SNLTVTTETAPSTALVVDEVAFAVERAAGEVCDRCRRIDTSVAERSYGATICDHCASV
                     VEENFAEAVAQGFEA"
     gene            complement(6921..7364)
                     /locus_tag="AC1PIW_001322"
     CDS             complement(6921..7364)
                     /locus_tag="AC1PIW_001322"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6606256.1"
                     /translation="MIRLELVNKDNFEVVLQVQLASEDQRRVASVEYSLAQAWLYKDS
                     GMILPYAVVSGRKVVGFAMLSIEPKDNSYYLWRLLIDKDFQNRGCGKEAIQQIIGKAK
                     ADPLCHKISINYVIGNHKMRYILEKIGFQSVGLEGQEMKMELIIK"
     misc_binding    complement(7386..7587)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7594..8283)
                     /locus_tag="AC1PIW_001323"
     CDS             complement(7594..8283)
                     /locus_tag="AC1PIW_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HIP6606257.1"
                     /translation="MALTALELKDKTFATKFRGYDADEVDDFLDIVTRDYEDLIRKNH
                     DQELELKNLRERLAYFDEMKESLSKSVLLAQDTAEKVKVAAEDQAANIIKQADYDAAT
                     LLHEAKDKANEILRNATDNAKKVVIETEELKNQTRIFHQRLKSTVESQLSLVNSSEWE
                     EILRPTASYIQTSDEAFRDVLHKALDEELPVEEESLDYTRQLTPEEIAELTRQAAAFE
                     SGESVVLSIEE"
     gene            complement(8292..9083)
                     /locus_tag="AC1PIW_001324"
     CDS             complement(8292..9083)
                     /locus_tag="AC1PIW_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HIP6606258.1"
                     /translation="MKNDKNLLQHFSREEREFVEKIMDMCQQVEDTYSYRLTHFLHPR
                     QDEIVCKIANYYQLQTFSSRDLVSTEHSRVIIAPTYYELDIKDFELTALHLSYARKFH
                     TLSHSQVLGTFLNQLGIKREYLGDILIDDERLIVFIDQKFGQIALQSITKVARVPVKG
                     KEEEWTTVRLPIGQEWRSKDVLVSSMRLDKLISVAFNLSRATANKLIEAGHVKLDYVL
                     TEQTSKLVEIGQLISVRRYGRVRLNEFLGFSKQGKIKLKLDIVKT"
     gene            complement(9086..>9280)
                     /locus_tag="AC1PIW_001325"
     CDS             complement(9086..>9280)
                     /locus_tag="AC1PIW_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HIP6606259.1"
                     /translation="LLSWFPSLYTSSIGRLIQWLVAPILKPFRRLNLQFMGLDWTVMA
                     AMIALNMGTRFLVQLLLLLA"
BASE COUNT         2826 a         2078 c         1739 g         2637 t
ORIGIN      
        1 attcaattta cacaagaaaa aagcaccctt aggtgcctct cacttcttct tagtacaata
       61 gctcatagat ttccattgca atcaagtcga tgctgtcaaa cgcgtacgtt tcacgagcag
      121 caacatcacg caaggtaaat actgaaccat tttcctcgtc atagctataa gccacttctg
      181 caacaacaat accatcacgt tcgaaattac gtttcaacgt accgccgtca ttagccatag
      241 cctccagacg attgataatt ctcaccaaat gtgattccat attcgttctc ctaatccttt
      301 ttataggaat attttaccac atattaccaa aaaagtctta ttttttccct attttcttcc
      361 tatcgcccta tttttcatag aaagtttttc caagcgtagc agtttcttga tttttctatg
      421 aatcttgtta taataaagct agaactgacc ttttttgact atttaacaat aggtcacatt
      481 ttttcacaga aagaggtaga attatgctct gcaaaaattg taatatcaac gacgcaacga
      541 tccatctcta caccaactta aacggtaaac agcagcaggt agacctctgt cacaactgct
      601 accaaatcat gaaaacagac cctcataatg ctatcctacg tgggttggga gatttaacaa
      661 atcctaacaa tatggatccc tttagtgaat ttttcaatca cctaggtggc tatcctggca
      721 atacccctgc tggaaaaaat cgcgaacaga ctcctcccac tcaagccggt gggcataatg
      781 gacgtggtgg acagacccct cctccgcaac aaccccaaca accaaatggg ctccttgaag
      841 aatttggtat caacgttacc gaaattaccc gtcgtgggga tattgatcca gtaatcggtc
      901 gcgatcaaga aattacccgt gtcatcgaaa ttctcaaccg tcgtacaaaa aacaatcctg
      961 ttcttatcgg tgagcctggg gtaggtaaaa cagcggttgt tgaaggcttg gctcaaaaaa
     1021 ttgtcgatgg cgatgtccca caaaaattac gagacaagga agtcattcgt ctggatgttg
     1081 tcagcctcgt acaaggaaca ggtatccgtg gacaatttga agaacgcatg caaaaactca
     1141 tggaagaaat ccgtaatcgt cgcgaaatca ttctttttat tgacgagatt cacgaaattg
     1201 tcggtgctgg ctctgcaggc gatggtaaca tggatgcagg caatatcctc aaaccagccc
     1261 tcgcacgtgg tgaaatgcaa ctagtcggtg caacaacgct caacgagtac cgtattatcg
     1321 aaaaagatgc cgccctagaa cgccgtatgc aacctgtaaa agttgaagaa ccaagtgtcg
     1381 aagaaaccat taccatcctc aaaggtatcc aaaataaata ccaggattac catcatgtta
     1441 aatactccga tgctgctatc gaagctgccg cagtactgtc caatcgctac attcaagacc
     1501 gtttcctacc agacaaggct attgaccttc tggatgaagc tggttcaaaa atgaatttga
     1561 ccctcaactt tattgatcca aaggaaattg accaacgcct gattgattcc gaaaatcgta
     1621 aagcacaagc tacacgggat gaggactatg aaaaagcagc ctatttccgt gaccaaattg
     1681 ctaagtacaa ggagatgcaa aaggcaacta tcagcgaaga agatattcca ctcattactg
     1741 aaaaagaaat tgaagccatc gttgagcaaa aaacaaatat cccagttggt gatttaaaag
     1801 aaaaggaaca gtcccagctt gtgagcctcg ctagtgactt aaaagcccat gttatcggac
     1861 aagatgaagc agttgacaag atagctaaag ccatccgccg taatcgtgtc ggtctcggtg
     1921 ctccaaatcg tcccattggt tccttcctct ttgtcggacc aacaggtgtc ggtaaaaccg
     1981 aattgtctaa gcaattagct atcgaactct tcggttcggc tgacagcatg atccgcttcg
     2041 acatgtccga atacatggaa aaacacgctg tcgctaaact cgtcggagct cctccaggct
     2101 atgtaggcta tgaggaggct ggacaattga ctgaaaaagt tcgtcgtaat ccttactcac
     2161 tcatcctcct tgacgaagtt gaaaaagccc acccagatgt catgcacatg ttccttcagg
     2221 tcttagatga tgggcgtctg acagatggac aaggtcggac agtcagcttc aaagatacca
     2281 ttatcatcat gacatcaaat gctggtactg gaaaagttga agcaagtgtc ggttttggtg
     2341 cggctatgga agggcggact caatcagtcc ttggccaact tagcaatttc ttcacacctg
     2401 aatttatgaa ccgatttgac ggtattattg agttccaacc actgagcaag gaaaacctac
     2461 ttgaaatcgt cagcctcatg ctagacgatg taaacaaacg cctttctcac aatggcatca
     2521 gcctccatgt aacagataaa gttaaggaga aattagtgga ccttggctac aatccaaaga
     2581 tgggagctcg tccactacgc agaaccatcc aagatcagat tgaagatgct atcaccgact
     2641 tctacttaga acatccagca gaaaaagacc tacgtgctgt catgtcaagt aaaggtacaa
     2701 ttcaaatcaa ggcacaaaca aagacaaaat aagaaaatag agctgctctc tacgagtagc
     2761 ctattttact gttttgctac tctatttttc taaattcttc cccatctcca tcctataaaa
     2821 aaactttact ttgtaaagcc tatcacaagg aaaaataata aaaaagtggt ataatgtata
     2881 caactcattc caataatgaa agaggataga attgcctatg gagaaaattg ctgtatttgg
     2941 agaaaagaag gctggcgtca gctaccagag tcgctttggt gtttacgcag ttatcccaga
     3001 tgaaaagaaa gaacagatca tactcgttca agcaccaaac ggagcttggt ttcttcctgg
     3061 tggagaaatt gaaaagggag aaaatcacct cattgctttg gagcgcgaat tgatggaaga
     3121 gcttggattc accgctgaaa tcggaaaata ctttggtcag gctgacgaat acttctactc
     3181 tagccaccgc gatacccatt tctataatcc agcctacatc tatgaagtaa ctagctacca
     3241 tcaaatagga caaccactag aagatttcaa ccatattgct tggttcccag tcaatgaagc
     3301 catcgataaa ctcaaacgtg gtagtcacaa atggggcatt gaacaatgga aattgcaaga
     3361 aaattcattc aataactagt aaaaacactc taatcgtgct ataatgtagc caaagagata
     3421 gaggaggttc cacatgaaaa tcattaacac gacaaatagt catcccaatc ttgtccaaag
     3481 ccagttggct aatactgatg ctttcctagt agagacttat tctgcaggta atacagacgt
     3541 tattttcaca caggcacccc gtcattatga actcttgatt agtaataaat accgtgcagt
     3601 acaacaaaat gaaatcgagc aaattcgtga tttcttcctt catcggaaaa ttgatgaacg
     3661 aactattaac aaggctgcca ttcaaacgat tcacacagac cgtttgattg aaatgtctat
     3721 tcccattatc gcagaagtgt aagaagccat ctggcttctt tcagattgaa gaaaaggtcc
     3781 attttggaca attgtcttca atcttttttc tttatgttga aaataaaaaa ctctcagaaa
     3841 cgctgaaata tcaatgtttc taagagttta atcacttgct atctttcgca aacctcttca
     3901 attttttata tttttggagt ttgtctacgt tctgtaagaa gccagatggc ttcttttttt
     3961 ctatttatac tcaatgaaaa tcaaaagtag cctaggaaac gaagccgaag atagaactgg
     4021 agttcatcaa ggctcttgac aacgaataat tttgattttc gaaaagtatt acaaaaaaag
     4081 cctatcgcta gatagacttt aacaaatgta ttgtgattgc ttaggcttca aaaccttgag
     4141 ctactgcttc agcaaagttt tcttccacta cgcttgcaca gtggtcacag attgttgcgc
     4201 catagctgcg ctctgcaaca ctagtatcaa tacggcggca acggtcacat acttcaccgg
     4261 ctgcacgttc tacagcaaag gcaacttcat caaccacaag cgctgttgat ggggcagttt
     4321 cagttgttac tgtcaagttt gaaacgatga gcaactgagc aaggtctgca ttcaagctct
     4381 ccaagaagga cttggtttca gctgagacat aggcagtcaa atgagcttca agtgacttac
     4441 caatcacttt ggcattgcgt gcttcttcca aggctttttg tgcctgagta cggaagacca
     4501 taaagttttc ccaatcagct aaaagctgcg cttcattagc aaaggtttcc acctctggca
     4561 tttccgccaa ctgaacaaat tcttctgctt cgtgttccaa gtaagaccag atttcttctg
     4621 ccgtatgtgg taagattggc gtcaagagct tggtaatttt caccaagatg tcatagaaga
     4681 cagtttgcat ctgacgacga gaaagtgatt tagcaccatc aatgtagacg acatctttgg
     4741 caaaatcgag gtagaaggct gacaaatcaa gggttacaaa gtttactaca gatttgtaga
     4801 tgcccatgaa atcataattg tcgtaggctt cacggatttg aactaccaat ttattgaatt
     4861 tcaccaagag atactggtca acagaacgaa gctcttcgta tgccaccgca tcacttgcag
     4921 gattgaaatc cgaagtgttg gcaatcaaga agcgaagggt attacggatt ttacggtagg
     4981 tttcagaaac ctgtccaaga atatccatag acacacggac atcgtttgaa gtatcaacgg
     5041 atgtcaccca cagacgtaag atttctgcac caaattgttt ctcaacatca cttggtagaa
     5101 tggtattgcc tagagattta gacatcttca tcccattacc atcaaggaca aaaccttgtg
     5161 acaagatagc tttatatggc gcatgaccat ttacagcaac agaggtaatc aaggatgagt
     5221 taaaccaacc acggtattgg tctgaacctt caaggtagag atcagctggg taggcaaggt
     5281 tttcacgggc attcatgaca ccattccaag atgaaccaga gtcaaaccat acgtccatga
     5341 tgtctgtttc tttggtaaat tcgccatttg gtgaacctgg gtgagtgaaa ccagctggta
     5401 aaagatcttt tgcatcagat ttccaccaga tgatagaacc atgttcctca aagagagctg
     5461 ctacatggtc tgttacttcc ttggtcataa tagctgtgcc atcttcagca tagaagattg
     5521 gaagcggcac accccaagca cgctgacgag agataaccca gtcaccacgg tcacggatca
     5581 tgttgtaaag acgcgtttta ccccaagatg gattgaaagt tgttgcttcg atttggtcca
     5641 agatttcctg acggaattta gaaacagagg caaaccactg tggcactgca cgccagatga
     5701 ttggcttctt ggttctccag tcaaatgggt aggagtgatt gatcacttca ctagcaagaa
     5761 gcaagtcacc caatttttcc ttaaccgttg gcacaacctt gtcatagaat tgcccttcaa
     5821 aatcaggacc cgcagcttca ttcatcaagc cacgttcgtt gacagttacc gcaacctcca
     5881 agtcatattt gacaccgaca ttgtagtcat cctcaccaaa gcctggagcc gtatggacga
     5941 taccagtacc tgagtccgtt gtaacatggt cacccaagat gaccaattca tccacttctc
     6001 tatcccatgg gtgttcggtc acgatatatt caaattcagc acccttgtgt tttgaaatca
     6061 cttcgaagga ttcccaaccg aatttggctg ccaagctatc aaccaaggct tctgccaaga
     6121 catacttacg gtcagagcca gcaggttgaa ccaagacata gtcaatgtct gctcccattg
     6181 tcaaaccgcg agaagccgtt actgtaaatg gagtggttgt ccagacaacg atgtagctgt
     6241 ctgtatccaa gagccccttg ccatctttga ccttattggc ataatacaga gatgttgagt
     6301 caatgtcatg gtattcgatt tcagcttctg caagggcaga ttcagatgac catgaccagt
     6361 agacaggctt agcaccgcgg tagatatagc ccttgtctgc catagcaccg aagacacgga
     6421 tctgagctgc ttcgtagtct tttgtcaaag tgatatatgg attttcccag tctgcagaaa
     6481 cacccaagcg tttaaaatca tcacgttgct tgtcaacctg gctaaaagcg tagtcacgac
     6541 acatttcaag gtattcaacc aagtccattt ccttgcgttt gacaccttgt tttgccaaga
     6601 cttgctcaat aggaaggccg tgtgtatccc aacccggtac aaatggagca cggaaaccag
     6661 acattgactt agagcgaacg ataatatctt tagaaatctt gtttaaagca tgtccaacgt
     6721 ggatatttcc gttagcatat ggagggccat cgtggaggtg gaaggctggt ttgccttcat
     6781 tgagttcttg gcgacgagca tacaagtttg cttcatccca ggccttttgc cattctggct
     6841 cacgtgttgg taagcctgca cgcataggaa atgctgtctt acctaaattg agtgtttctt
     6901 ttagtttcat cataactcct ttattttata attaattcca ttttcatttc ttggccttct
     6961 aaaccaacgg attgaaaacc aattttttct aaaatgtacc gcattttatg attgccaatg
     7021 acataattta tcgaaatttt atgacaaagc ggatccgcct tcgcctttcc aattatttgc
     7081 tggatggctt cttttccgca tccacgattt tgaaaatcct tatcaatcaa caagcgccat
     7141 aaataataac tattatcttt gggttcaata gataacattg caaaaccaac aacttttctg
     7201 ccagatacca cagcatatgg tagaatcatc cccgaatctt tatataacca agcttgggct
     7261 aaagaatatt caaccgatgc cactcggcgt tggtcctcag atgcaagttg aacttgtaac
     7321 actacttcaa agttatcctt gtttactaat tctaatcgta tcataaaaat aaaaaactcc
     7381 cgccaaataa ggacgaaagt tcgtggtacc accttagttt agatagagtt tcctctatcc
     7441 ctctatgttc gtaacgtgaa cggacgtctc tccttactat tttcaggaaa gatcaaacaa
     7501 agtgatgatc catagaaaga gattgtaggg cttccaccat ctcctactcg cttaaaatat
     7561 ttctaacgac cttgtctttg cttatgattt ctattattct tctattgaaa gtactacaga
     7621 ttctccactc tcaaaagctg ctgcctgacg agttaattct gcaatctctt ctggtgtcaa
     7681 ttgacgtgta taatccaaac tttcttcttc aacaggtaat tcttcatcca aagccttatg
     7741 aagaacatca cgaaaggctt cgtcacttgt ttgaatgtaa cttgctgttg ggcggaggat
     7801 ttcctcccat tcagatgaat taaccagtga taattggctt tctactgttg attttagacg
     7861 ctgatggaaa atacgtgtct ggttttttaa ttcttcagtt tcaatgacaa cttttttcgc
     7921 gttgtcagtc gcattacgaa gaatttcatt tgccttatct ttagcttcat gtaacaatgt
     7981 tgccgcatca tagtcagctt gtttaataat atttgccgct tgatcttcag ccgcaacctt
     8041 tactttctca gctgtatctt gagctaaaag aactgattta ctcaatgatt ctttcatctc
     8101 atcaaaataa gccaaacgtt ctcgcaaatt tttcaattct aactcttggt catgattttt
     8161 acgaattaaa tcctcataat cacgtgttac aatgtccaaa aagtcatcaa cttcatctgc
     8221 gtcgtatcct ctaaattttg tagcaaaggt tttatctttt aattctaatg ctgtaagtgc
     8281 catctttcct cctaagtttt aacaatgtct aactttaatt ttatcttccc ttgcttagaa
     8341 aaacctaaaa attcatttag gcgaactcta ccatatctcc taacactaat taattgccct
     8401 atttcaacta atttactggt ttgttctgtc agaacataat ccaacttcac atgtccagcc
     8461 tcaatcaact tgtttgcagt tgcccttgaa agattaaatg cgaccgaaat caatttatcc
     8521 aatctcatac tagaaaccaa cacatccttt gaacgccatt cctgtccaat cggtagtcga
     8581 acagtcgtcc attcttcttc ctttccctta acagggacac gagcaacttt agtgatagat
     8641 tgtaaggcaa tctgcccaaa tttctgatcg ataaagacaa tcaatcgttc atcatcaatc
     8701 aaaatatcac ccaaatactc ccttttaatc cctagttgat tgagaaaagt tccaagaact
     8761 tgtgaatggg ataaggtgtg aaattttcta gcataggaca aatgaagagc ggttaactca
     8821 aagtccttga tgtccaactc ataataagtc ggggcaataa taacccgcga atgttctgtc
     8881 gaaacaaggt cacgactaga aaacgtttgt aactggtagt agttagcaat tttgcataca
     8941 atttcatctt gtctaggatg taaaaagtga gttaatctgt atgaatacgt atcttcaact
     9001 tgctgacaca tatccattat tttctctaca aactctcttt cttccctaga aaaatgttgc
     9061 aataagtttt tgtcattttt cataactatg ctaacaggag caataattga accaagaagc
     9121 gtgttcccat attaagcgca atcatggctg ccattacggt ccaatccaac cccataaact
     9181 gcagatttaa ccgcctaaag ggttttaaaa tcggtgctac cagccattga atcaaacgac
     9241 caatcgagct tgtgtatagg ctaggaaacc aacttagcaa
//
LOCUS       DBNFCK010000070         9226 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.070, whole genome shotgun sequence.
ACCESSION   DBNFCK010000070 DBNFCK010000000
VERSION     DBNFCK010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9226
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.070"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..372)
                     /locus_tag="AC1PIW_001326"
     CDS             complement(<1..372)
                     /locus_tag="AC1PIW_001326"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HIP6606260.1"
                     /translation="MSEVRWQIKSLSELGRFSRGKSKHRPRNDKKLFTNGTYPLIQTG
                     DIKNSNLYVTKNSDYYNEFGLSQSKLWKQGTLCITIAANIAETAILSYPMCFPDSVVG
                     FNAHKNESSELFVYYVFELIKK"
     gene            complement(365..2006)
                     /locus_tag="AC1PIW_001327"
                     /pseudo
     CDS             complement(365..2006)
                     /locus_tag="AC1PIW_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638904.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
     gene            complement(2011..5064)
                     /locus_tag="AC1PIW_001328"
     CDS             complement(2011..5064)
                     /locus_tag="AC1PIW_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HIP6606261.1"
                     /translation="MIRKEHNEFTRVQLPAALHLTRLGYDYLSATSEDIKNRDHSTNI
                     LLSIFKRQFLTFNNYASEADFEQEFENIRLELEQDDLGRSFFNRIHADSGYTYINWDN
                     MEENTFHIALEVTCQNGEEEFRPDITVFINGLPLSYIEVKQPDAIRDGKTGIHSEKER
                     AKLRFENKKFRRFNNITQLIAFSDNLPYDDSQGQQLQGSYYCTNASSGTKFNSFKEEQ
                     EGQVQSSLSSLTEEMIDLVLTDVNRFALKSQVEFRTNLDPASPCNSFLTSLYQKERLF
                     FMLRYGLVYVEERDEHDQIQLQKHAMRYPQYFATKAIERGLEEGMKKGVIWHTQGSGK
                     TALAFFNIRYLRDFYAKRGVVPQFYFVVDRLDLADQAYKEFSKRGLKVKRINKPSELN
                     QIQDQHHVSVVNIQKFKESSDLTDHSGYDLNRQNIYFIDEAHRSYNEKGSYLPNLYNA
                     DKEAVKIALTGTPLIATKADGKTKETAVTTRDIFGDYIHKYYYNQSIADGFTLRLMRE
                     DIETSYKETLQAVHEEIQKGSIEKKEIFAHPRYVSPMLDFILDDFTRAREILFGDQSI
                     GGMVVCDSSEQARELHKQLEDRRKAGLTSFTSTLILHDAGDKEYKKEEVDKFKDGLID
                     FVIVYSMLLTGFDAPRLKRLYLGRKIKAHNLLQTLTRVNRPYKDYAFGYVIDFADISK
                     EFDKTNKAYLEELNREYQAALTEEDGDNPFGTIFVSPEEVQEQLASSNRVLMNYPTDN
                     LEFFGRAIDEVKDRKELIALRKSLETIRQYYNMARLHGYHDIVKQVNIGEIASLLNML
                     SRRLLTLNLLEKPDSFSSQQLLNLAMSETSFSFTKIKEEELRLAANDLDDIRRRVANG
                     INLRRDEKDPEWVSLYEEFQRILNKHMTQEVEGYSLSTIKETKQAYQSLFDSVEDYKT
                     RMNRLAMNFGGDTMSARAFKHITQSTVVSDFPAIYQVLKGAKPLIDYQIGLNQGILEN
                     EAYLVAQIRQLARKEMMKTEVGKQLKRVDYDKLIRSLMEVYEGEY"
     gene            complement(5157..6473)
                     /locus_tag="AC1PIW_001329"
     CDS             complement(5157..6473)
                     /locus_tag="AC1PIW_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HIP6606262.1"
                     /translation="MEQYDLVVIGFGKAGKTLAGKLSAAGKKVALVEENPVMFGGTCI
                     NIGCIPTKTLLVAADKNWTFEQVMEQKETVTTRLRNKNEAVLKGSGANLYQGHARFVA
                     DKVVEVSAGEESIQLTAETIVINTGAKSRVLPIPGLLDTAHVYDSTGIQNLETRPNKL
                     AIIGGGNIGLEFAGLYSKLGSQVMVYEASSAILPREEEGVAQLAKEYMEEAGMTFVLG
                     AKIEQVAAAGEQVAVTVNGETAIFDAVLYATGRVPNTADLGLENTAIELLENGAVKVD
                     DYRETTVPGVYAVGDVNGGPQFTYTSLDDFRIVFGKLTGTGTYSLSQRKSIPTSVFIT
                     PVLSRVGLTEKEAKEAGYDYIANELLVANMPRAHVNNDLKGIFKVIVDKESKLVLGAT
                     LFGRNSEELINLIAMAIDNKIPYTYFKTQIFTHPTMAENLNDVFNF"
     gene            6688..7497
                     /locus_tag="AC1PIW_001330"
     CDS             6688..7497
                     /locus_tag="AC1PIW_001330"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018381403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HIP6606263.1"
                     /translation="MKEKFQMCKTYLPVVLATIGFVNGCKIIFGELGKPNGMEAKYSL
                     FLLTISLVAIYLIPLLVLTYSLAKRYNISKQVIGLSWLLGLTSSISFSELGHTAIGYF
                     LLEIVKASNDFLNDWGAAISGPLAEEIGKGLTVLLVLLICRKMTLKNALVSGVIVGLG
                     FQIMEDITFVFRDMFMNKLDGFETILERVGQAGWAHWVFTLLFAIGLVALLTKNTGMS
                     KAQGVFWIGASFGIHFLFNSPFNTGIFQTVFPILSISLGLLAYRTVEKLSE"
     gene            complement(7541..9211)
                     /locus_tag="AC1PIW_001331"
     CDS             complement(7541..9211)
                     /locus_tag="AC1PIW_001331"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HIP6606264.1"
                     /translation="MKTDIDIAQSITLKPITEIVEKVGISFDDIELYGKYKAKLSFDK
                     INAVKDNAPGKLILVTAINPTPAGEGKSTITIGLADALSKIGKKTMIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHIHQGNVIGIDQRR
                     IIWKRVVDLNDRALRKVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDINDLKER
                     LANIVIGYRFDCSPVYVRDLAVEGALTLILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALRLADYTVTEAGFGADLGAEKFLDIKVPNLPKAPDAVVIVATLRAL
                     KMHGGVAKTELSAENVEAVKAGFSNLKRHVENIRKYGIPAVVAINEFVSDTAAEIAVL
                     KELCAAIGVPVELASVWANGADGGVELAETVVATIDNQAASYQRLYKSEDSLEEKVTK
                     IVTQIYGGTGVVFEKKARNQLTEFAKNGWDKLPVCMAKTQYSFSDDQFALGAPTDFDI
                     TVREFVPKLGAGFIVALTGDVMTMPGLPKAPVALNMDVAADGTAIGLF"
BASE COUNT         2559 a         2047 c         1832 g         2788 t
ORIGIN      
        1 tttttttatt aattcaaaaa cataataaac aaacaactca cttgattcat ttttatgtgc
       61 attaaaacca acaacactat caggaaaaca cattgggtaa gatagaatcg ctgtttcagc
      121 aatatttgct gctattgtaa tacaaagagt cccttgcttc catagcttac tttgtgacaa
      181 accaaattcg ttataataat cactattctt tgttacataa agatttgaat ttttaatatc
      241 acctgtctgg attagcggat aggttccatt agtaaataat tttttatcat ttcttggacg
      301 atgtttagat tttccccttg aaaatcttcc cagttcagac aaactcttta tttgccatct
      361 tacctcactc atacctcacc cccctcacct gctccgctat ttccttttcc aattcatgcg
      421 attgggcaaa gaggttagct aggcggtctt ggtagccctg cattttttct tcaaattctt
      481 ctgggctaat atcaacgtag tcaatcttga tgtcaaagta ctgacctgca ctgagggagt
      541 agttcttatc cttaatctct tcatagctaa ccttgacaga gaagtcttcg acaacttctt
      601 tgttaataaa ggtctgaatg atttggcttt cttcatcagg gctaaggact gtcttttggt
      661 tcttgccttc cttgaccttg gtgccaagat tagaagcgtc aatcaagacc acatcgtcct
      721 tattcttctt atcgataaag agaatggaca cattggtgcc tgtggtcgca aagatattgg
      781 acggcatgga cacaacgcct gccaacatct tttcatcgac aaggtgctga cggattttct
      841 tgtcaatacc tgactgggcg gtgataaagc ccgtcggcaa gacaatagct gcctgcccgt
      901 cttccttcaa actatggata atatgctgga taaagagttg gtaaatagcc atactctctt
      961 ttttcttgtt gggaatcttt ggcacacctg caaagaaacg ttcactgcta tttggcaggc
     1021 tttctacctg atctcgccac tcagaaaaat ctaatttgaa aggaggattg gacacgatat
     1081 agtccatctt ctccacatga cgattgttga gaatggtatt tccctgtacg atattgtgaa
     1141 tggaatgctg caagccattg agaatcaagt tgagacgcaa gagattggag gatttctgag
     1201 aaatatcctg actgtaaacg ctggtacggt cggtaccaat ctgactggcc agattcatca
     1261 ccaatctgac tggccagatt catcaagaga gtccccgaac cagcagaagg atcatagatt
     1321 ttgacattac ttggttggtc attgcccacc aaaatggccg cgatgatttt ggcaacagag
     1381 tgaggtgtgt agtattccgc atactttcca ccaccgtctt tgttatagtc cttaatcatg
     1441 tactcaaaca aggtggagaa aaagtcaaag ccctgtgaaa aaatctgctg atcaaactta
     1501 acacgcgcca agagattgat aatagaagct gccacttcat ttcgcttgct ggcatctgaa
     1561 atcgtatccg taatcaagcg ttgatcaaag aggcgaatat ctgttccacc gtccgtatgg
     1621 acagagaaaa tagcattgtt gtcaatagca atctgattga gagtctgttc aaaaatctca
     1681 gcaaaatctg cttggttctg ttgacggtgt aggctttcaa tcagctgttc aggttttaga
     1741 tgagcagttt tagtcccaat atcatctaaa aaccagtcat aatcttcttc agacaagtcc
     1801 aaaagacctt gatagtcata acgattgtca acgatcaagg cttcatacaa aaacttgtca
     1861 tttaagaact tgtaaaggaa ggactgggtc agaagtttgt actcccccgc ttctcctcca
     1921 agtcctgcat gggtaaagac ggcttttagg tcatcaacca agccttgtac ttgactttgc
     1981 acctgcacga tataatgttc ttccatttgt ttaatattct ccttcataga cttccattag
     2041 ggaacgaatc aacttatcat aatcgactcg tttgagttgc ttccctactt ctgttttcat
     2101 catttcttta cgggccagtt gcctgatctg cgctaccaaa taggcttcgt tttccagaat
     2161 gccttgattg agcccgattt gataatcaat cagaggtttg gcacctttta gcacctgata
     2221 aatagctggg aagtcactga caactgttga ttgggtaatg tgcttaaagg cacgggcaga
     2281 catggtatct ccaccaaagt tcatggccaa gcgattcatt cttgttttat agtcttctac
     2341 tgaatcgaat aaactttgat aggcttgttt ggtttcttta atggttgaga gactatatcc
     2401 ttctacttcc tgtgtcatgt gcttgtttag gatgcgttga aactcttcat aaagacttac
     2461 ccattcagga tctttttcat ctctccgtag attgatacca ttggccacac ggcgtcggat
     2521 atcgtccaaa tcattggctg ccagtcgtag ttcctcttcc tttatcttgg taaaactaaa
     2581 gctagtctca gacatggcta gattcagtag ttgctgacta gaaaaactat ctggtttttc
     2641 taacaagttc aaagtcaaga ggcggcggga cagcatattt aaaaggctag caatttctcc
     2701 tatattgacc tgtttgacaa tatcgtggta accatgcaaa cgtgccatgt tgtagtactg
     2761 acgaatggtt tcaagagatt ttcggagggc gattaactct ttcctatcct tgacttcatc
     2821 gatggctctt ccgaaaaatt ctaagttatc tgtcggatag ttcattagaa ctcggttaga
     2881 gctcgctagc tgttcctgga cttcttctgg ggacacaaaa atagttccaa aaggattatc
     2941 tccgtcttct tctgtcaaag ctgcctggta ttcccgattg agttcttcca gataggcttt
     3001 attggtcttg tcaaattcct tggaaatgtc cgcaaaatca atgacatagc caaaagcata
     3061 atccttgtac ggacggttga cacgggttaa ggtctgcaag agattatggg ccttgatttt
     3121 ccgtccaaga tagagccgtt tcagccttgg tgcatcaaaa ccagtcaaga gcatggagta
     3181 gacgatgaca aagtctatca gaccgtcctt gaatttatcc acttcttctt tcttgtattc
     3241 cttatcccca gcatcatgta aaatcaaggt tgaagtgaat gatgtaagcc ctgcttttcg
     3301 acggtcttct aactgtttgt gtaattctcg ggcttgttct gaagaatcac agaccaccat
     3361 tccaccgata gactggtcgc caaataagat ctctcgtgct cttgtaaaat catcaagaat
     3421 aaagtctagc attggagaga cataacgggg gtgggcaaaa atttctttct tttcaatgga
     3481 gcctttttga atctcttcgt gtacagcttg tagagtttcc ttgtaagaag tttcaatatc
     3541 ttccctcatc aaccgtaggg taaaaccgtc agcaatggat tgattatagt agtatttgtg
     3601 gatatagtcc ccaaagatat cacgagtcgt cacagctgtt tccttggttt taccgtcagc
     3661 ctttgtcgca atcaaaggcg ttcctgtcag tgcaatttta acagcttctt tatcagcatt
     3721 atatagattt ggcagatagg aacctttttc attgtaagaa cggtgagcct catcaatgaa
     3781 gtagatattt tgccgattca agtcatagcc tgaatgatct gtcaaatcag aggattcttt
     3841 gaatttttgg atgttgacaa cagaaacatg gtgctggtct tgaatctgat ttaactcgct
     3901 tggtttgtta attcgtttga ctttgagacc acgcttgcta aattccttat aggcttgatc
     3961 agccaaatcc aatcggtcta ccacaaagta aaactgcggg acaacacctc ttttagcata
     4021 aaagtctcgt aagtatcgga tattgaaaaa tgctagagcg gtttttccag aaccttgtgt
     4081 atgccaaata acacctttct tcatcccttc ttccaatcct cgctcaattg ccttggtcgc
     4141 aaaatattga ggatagcgca tggcgtgttt ttgtagttga atctggtcat gctcatctcg
     4201 ttcttccaca tagactagac catagcggag catgaaaaag agccgttctt tctgataaag
     4261 ggaagttaaa aaggaattgc aaggacttgc aggatccaag ttggttcgga attcaacttg
     4321 cgacttgagg gcaaaacgat tgacatctgt gagaaccaaa tcaatcatct cttctgttaa
     4381 gctagacagg ctagactgta cttgtccctc ctgctcttcc ttgaaagagt tgaacttggt
     4441 accagatgaa gcattggtgc agtaataaga accttgcaac tgttgtcctt ggctatcatc
     4501 atagggaaga ttgtctgaaa aagcaatcaa ctgtgtgata ttgttaaacc gtctgaattt
     4561 tttattttca aagcggagct tggcacgttc tttttcagag tgaattcctg tttttccatc
     4621 acgaatagca tctggttgct tgacttcgat ataactgaga gggagaccgt tgataaaaac
     4681 ggtgatatca ggccggaact cttcctcacc attttgacag gtgacttcta aggctatatg
     4741 gaaggtattc tcttccatat tatcccaatt gatataggtg tagccagaat ctgcatgaat
     4801 ccgattaaaa aaacttcgtc ctaaatcatc ttgttctaat tccaggcgaa tgttttcaaa
     4861 ttcttgttca aaatcagctt cacttgcgta attattgaat gttaaaaact gtcgcttaaa
     4921 aattgataga aggatattgg ttgaatggtc tctgtttttt atgtcttcac ttgtagccga
     4981 aagatagtca tatccaagcc gagtcaaatg caaagcagct ggcaactgaa ctcttgtaaa
     5041 ctcattgtgt tcctttctta tcatacaact ctcctagtct tttcttatca atcattatac
     5101 catatttgca agtgctgacc gacaaaaaac cgccctattg gacggttatt acactattaa
     5161 aaattaaaga catcgttcaa attctcagcc atagtcggat gggtgaagat ttgtgttttg
     5221 aagtaggtat aaggaatctt attgtcaatc gccatagcaa tcaagttaat cagctcctct
     5281 gaattgcgac caaagagagt cgcccccaga acaagcttac tttccttatc cacgataacc
     5341 ttgaaaatgc ctttgaggtc attattgaca tgggcacgtg gcatattggc gacaagcaat
     5401 tcgttggcaa tgtagtcgta acctgcttcc ttggcttcct tctcggtcag accaacacga
     5461 gaaagcacag gcgtgataaa gacgctggtc ggaatagact tacgttggct caagctataa
     5521 gttccagtcc cagtcaactt gccgaaaacg atacggaagt cgtctaggga tgtgtaagtg
     5581 aattgtggac cgccattgac atctcccacc gcatagacac ctggaacagt cgtttcgcgg
     5641 tagtcatcca ccttaacagc accattttcc aaaagctcaa tcgccgtatt ttccaagccc
     5701 aagtcagcag tgttcggcac ccgacctgtc gcatagagga cagcgtcgaa aattgctgtt
     5761 tcgccattga ccgtcacagc aacttgctcg cccgcagctg cgacttgctc gattttcgca
     5821 cccaagacaa aggtcatgcc agcttcttcc atatactcct tggctagctg agcgactcct
     5881 tcctcttctc ttggtaaaat agcagagctg gcttcatata ccatcacctg gctgccgagt
     5941 ttgctgtaaa gtccagcaaa ttccaatcca atgttaccac caccgataat tgccaacttg
     6001 ttaggacgag tttccaagtt ctgaatgcct gtactatcat aaacgtgagc tgtatccaac
     6061 aaacctggaa ttggaagcac gcgcgactta gcacctgtgt tgatgacaat agtttccgca
     6121 gtcagttgaa tcgactcttc accggccgac acttcaacga ctttgtccgc aacaaaacgc
     6181 gcgtgacctt gataaagatt agcaccactg cctttcaaga ctgcctcgtt tttgtttcgc
     6241 agacgagtgg tgactgtttc cttttgctcc attacctgct caaaagtcca gtttttatct
     6301 gctgctacca agagggtctt ggttggaata cagccaatat tgatacaagt cccgccaaac
     6361 ataacaggat tttcctcaac caaggcaacc ttttttccag ctgctgacag cttaccagcc
     6421 aaggtcttac cagcctttcc aaaaccaatg actactaaat catattgttc cataaaatac
     6481 tcctttattt tttcaacatt agtctatcaa aaaagaaaat ttttggcaca attctgctac
     6541 ggcctattat ggagtcctag ttgcaggaaa aaacattaca ataactgaga aggtttagat
     6601 aaaaaatagg tgaataatag acaaaatgta cttatttttg atacaatgta tccgtacact
     6661 agtttattaa tacagaaagg tcaaactatg aaagaaaaat ttcaaatgtg taaaacttat
     6721 cttccagtcg tcttagcaac gattggattt gtcaacggtt gcaaaattat ctttggagaa
     6781 ttaggtaagc caaatggcat ggaagctaaa tattctctct ttctcctaac catttccttg
     6841 gtcgctatct acctcattcc cctactagta ctgacatatt ccttagctaa acgctataac
     6901 atctccaaac aagttatagg gcttagctgg cttttaggct taacaagtag catctctttt
     6961 tctgaactgg gacatacagc tatcggatat ttcctgctcg aaattgttaa agctagtaat
     7021 gatttcctaa atgactgggg tgcagctatt tcaggtccat tggcagaaga aatcggtaaa
     7081 gggctaactg ttctacttgt actgcttatt tgtagaaaaa tgacactaaa aaatgcattg
     7141 gtaagcggag tgattgttgg attaggtttc caaatcatgg aagacatcac ctttgttttt
     7201 agagacatgt tcatgaataa attagacggc tttgaaacta ttcttgaacg cgttggtcaa
     7261 gctggttggg cccattgggt tttcacgctt ctctttgcca ttggcttagt agcccttctc
     7321 acgaaaaata caggtatgtc aaaagcacag ggagtatttt ggatcggtgc aagctttgga
     7381 atccattttc tctttaattc accattcaac actggtatct tccagacggt gtttcctatc
     7441 ttgagtattt cccttggctt acttgcctat cgaacggttg agaaactatc tgaatagaca
     7501 aaaaatccgc ctttgagcgg attttttata ttcattcttg ttagaacaag ccgatagctg
     7561 tcccgtctgc tgccacatcc atgttgagag ctacgggagc ttttggcaaa ccaggcatgg
     7621 tcatgacatc tccagtcaag gcaacgataa agcctgcacc caattttggc acaaattcac
     7681 ggactgtaat gtcaaagtct gtcggcgctc caagggcaaa ctgatcatct gagaagctgt
     7741 actgggtctt ggccatgcag actggcaact tatcccagcc attcttagca aattcagtca
     7801 gctggttacg agccttcttc tcaaagacaa cgcctgtacc accgtagatt tgcgtaacaa
     7861 tcttggtcac tttctcttct aagctatcct cagacttgta aagacgttgg tagctggctg
     7921 cttggttatc aatagtagca acaactgtct cagccaattc aacaccacca tcagcaccat
     7981 ttgcccaaac acttgccaac tcgacaggta caccgattgc ggcacagagt tctttgagaa
     8041 cagcaatttc tgctgcggtg tcggacacaa attcattaat ggctacaaca gctggaatac
     8101 catacttacg gatattttcc acgtggcgct tcaagttaga aaaaccagct ttcactgcct
     8161 ctacattttc agcagaaagt tctgtcttag ctacaccacc atgcatttta agtgcacgga
     8221 gggtggcaac aatgactact gcatccggag ccttaggcaa gttcggcacc ttaatgtcaa
     8281 ggaacttctc tgctccaagg tcagcaccaa atcctgcttc tgtgacggta tagtccgcca
     8341 aacgcagagc tgttgtggta gcgaggactg agttgcagcc gtgggcgatg ttagcaaatg
     8401 gaccgccatg gacaaaggct ggcgtgccat agatggtttg gacaaggttt ggtttaatag
     8461 catccttcaa aataagagtc aaagcacctt ccaccgccaa atcacgcacg tagacaggac
     8521 tgcaatcgaa acgataaccg ataacaatat ttgccaaacg ttctttcaag tcattgatgt
     8581 ctgtcgccaa gcataaaatc gccatgattt cagaagctac ggtaatatcg aatccatctt
     8641 cacgaggaat accattgagc ggaccaccca agccaactgt taccttacga agggcacgat
     8701 cgttgaggtc cacaacacgt ttccagataa tacgacgttg atcaatgcca atcacattcc
     8761 cctgatggat atggttatca atcaaggctg aaagggcgtt gtttgctgtg gtaatggcat
     8821 gcatgtcgcc tgtaaaatgc aaattgatgt cttccatagg taagacctga gcatagccac
     8881 cacctgcagc tccacctttg atccccatga caggtcccaa agaaggctca cggagggcaa
     8941 tcatggtttt cttgccaatt ttagacaagg catctgccaa accgatagta atagttgatt
     9001 taccttcacc agctggcgtt gggttgatag ctgtcaccaa gatcaatttc cctggcgcat
     9061 tgtctttcac cgcattgatt ttatcaaaag acaattttgc cttgtatttt ccatatagtt
     9121 caatatcatc aaaactgata ccaacttttt caacgatctc tgtgataggt ttcaaggtta
     9181 tactttgtgc aatgtcaata tctgttttca tgagagtacc taattt
//
LOCUS       DBNFCK010000071         9186 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.071, whole genome shotgun sequence.
ACCESSION   DBNFCK010000071 DBNFCK010000000
VERSION     DBNFCK010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9186
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.071"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(407..595)
                     /gene="rpmB"
                     /locus_tag="AC1PIW_001332"
     CDS             complement(407..595)
                     /gene="rpmB"
                     /locus_tag="AC1PIW_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HIP6606265.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(988..1869)
                     /locus_tag="AC1PIW_001333"
     CDS             complement(988..1869)
                     /locus_tag="AC1PIW_001333"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HIP6606266.1"
                     /translation="MPLVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKQAP
                     VLIQTSMGAAKYMGGYKVARNLIANLIESMNITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLAKAKEVVELAHAKGISVEAEVGTIGGEEDGIVGSGELAPIEDAV
                     AMVATGIDFLAAGIGNIHGPYPANWEGLDLDHLRKLTEAVPGFPIVLHGGSGIPDAQI
                     QEAIKLGVAKVNVNTECQIAFANATRKFAAAYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSANKA"
     gene            complement(2104..2189)
                     /locus_tag="AC1PIW_001334"
     tRNA            complement(2104..2189)
                     /locus_tag="AC1PIW_001334"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2153..2155),aa:Leu,seq:aag)
     gene            complement(2470..4074)
                     /locus_tag="AC1PIW_001335"
     CDS             complement(2470..4074)
                     /locus_tag="AC1PIW_001335"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIP6606267.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITDALKDKIKRAARTTDADVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGSDNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEQPAG
                     QGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNMQAQNMDQIVCDHLKLDVPPADMTE
                     WTAMVNKVLNLQKKVKIALVGKYVELQDAYISVVEALKHSGYANDAAIELDWVNANDL
                     TAENVAERLGQAQGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCVEFA
                     RHVLGLEGANSFELDPETKYPIIDIMRDQIGVEDLGGTLRLGLYPSKLKRGSKAAAAY
                     DNQEVVQRRHRHRYEFNNDFREQFEKAGFVFSGVSPDNRLVEIVEIPENKFFVACQYH
                     PELQSRPNRPEGLYTAFVSAAMENEK"
     gene            complement(4267..4848)
                     /gene="rpoE"
                     /locus_tag="AC1PIW_001336"
     CDS             complement(4267..4848)
                     /gene="rpoE"
                     /locus_tag="AC1PIW_001336"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HIP6606268.1"
                     /translation="MELNVFAGQEKSELSMIEVARAILEERGRDNEMYFNDLVNEIQN
                     YLEKSNSEIRAALPTFYSDLNVDGSFIPLGENKWGLRSWYAIDEIDEEVITLEEDDED
                     APKRKKKRVNAFMDGDDDAIDYGHDDPEDEDNYPGSVSSEYDDENPDDEKDEVESYDS
                     EINEIIPDDELDEEDVDLGEDDDEYSDEEVVDE"
     gene            complement(4975..8130)
                     /locus_tag="AC1PIW_001337"
     CDS             complement(4975..8130)
                     /locus_tag="AC1PIW_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HIP6606269.1"
                     /translation="MKKKAVVGSVAALVLGMSLCSYQLGRFQAMEEQKNRVSYIEDSQ
                     SVQTTVAEQLTPDQVSAKENIDAEQIVVKITDQGYVTSHGDHFHYYNGKVPFDAIFSE
                     ELVMKDPNYVLQDSHIINEVQDGYVIKVDGKYYLYLKDPSKHKNVRSKEEVERQKGIS
                     SADSKNQAAGNSKDGRYRTDDGYVFNPTDVIEDTGDGFIVPHGDHFHFIPKKDLSAAE
                     LKAAQDYWNQKGSVSSASGSQYGDRNNRAQQTTISAGQGQDLASLLAQLDATPLSQRH
                     VEADGLVFDPRTITKKTAAGVIVPHGDHYHFIPYSQMSPLEEKISRMIGVNGAGVSSG
                     AQASHSQHTLTQPNRPVTPIGTVTTQPVSPTQPVLPTQPKQSTGKVVSYKGRQIPAYG
                     KGLDGKAYFTSDGYTFSKDSITSVDDQGLIASHGDHFHYVGFGELEDFEIKQVEEWVN
                     EKAGKQVPPKTSEQVGNDAKPTTPSQGNDSKPVKPIQEENRPAFEYKQVTAKRKLAGK
                     VVYEMEVGGKTYTYGRDELDLMKISFAELTLAEKDKQYIFDIAPLAEGDLKPAMLVGM
                     DQIPMKGANATYDTGQSFIIPHIDHIHVLPYTWLSKEQIATIRYIMQHPEIRPSAWTT
                     SGHGDGEATDLVPPILNATPKANRLGLKNWQIIHTAEEVMDARAKGKFATNDGYIFSA
                     EDVLDPASFVFSQAFSLPRATGGSLRSISKKDLSKEELEAVQTLLDKRDAEELAKNVT
                     PIEKRAGLKNWQIVHSAEELAEAKAAGKYTTKDGYIFDPADLLDPKVKIGTDNYRIPR
                     VITDGYRRINKSDLNYLSELIPAEAMVAQREKSNSSLPSTPAPTETGASAGETTTPEQ
                     PQVAKETAEEIYNRVEAKKVVPFEALTYNAGYATEVRNGTLVIPHQDHYHYVSFKWFD
                     QGSARSPEGYSLEDFLATVKYYMTNPQERPVSDDGWGVFTPNTPSESTEETETEESDE
                     EIISEETEEIDEFTEELKRRAEEFGMDFKTFEQSLVTLSDRYKVSFEAFEYDAASKVV
                     RLVDKDGVKRTISLPSLEEQV"
     gene            complement(8164..9084)
                     /locus_tag="AC1PIW_001338"
     CDS             complement(8164..9084)
                     /locus_tag="AC1PIW_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter solute-binding protein,
                     Zn/Mn family"
                     /protein_id="HIP6606270.1"
                     /translation="MLKKVIRGCFVALFGFVLAACSAQKEASQVQPGMKIVTSFYPIY
                     SLVKEVSGNKNDVRMIGSRQGIHSYEPSAADIKAIYDADVFIYHSRILESWAGRLEPN
                     LQGSSVKVLEASTNLPLTKVPGLEDMEAGQGIDEASLYDPHTWLDPVLVGQEAVAIGE
                     LLAESDPKNADYYRQNAATLEGKAQKLADKYSPIFLKATSKTFVTQHTAFSYTAQRFG
                     LKQLGIAGVSEEEPSPRQLAEIKEFVDTYNVQTIFTEKGASDKLAKALASSTGVDLKV
                     LDPLEADPENNLTYLENLEQVLETLAQELK"
BASE COUNT         2392 a         2035 c         1672 g         3087 t
ORIGIN      
        1 gtttctctgt taactagtcc ctcaagagac aactcattta ttctatcatg acttatatac
       61 attgtcaacc ggttttttga gattttttgg atttttttaa acactctcta ttcttccagt
      121 tcgtgattcc cttcttgtag aacccgtacg gatgctctct cgtacaactc ctaatgtcaa
      181 tccaagttta aatgttcgaa cttggaaaaa tcttccacat caatacacag tttatttttg
      241 acaacattcc ttctttgtta acttttcaac agcaatgtga agagctatat agctgatatt
      301 ctttcctatt cattatgtag tggtcgaatt tgctcctcac cgactttata tctaggctat
      361 tttttccaaa aacaaaagtc gctagatttt tctaacgact ttgttattat acacgttcaa
      421 ctttgcctga ttttagtgca cgcgctgaag cccaaacttt tttaggttta ccatcaatca
      481 aaacagttac cttttgaagg tttggtttaa ctgcacgttt agtttggttc atagcgtgtg
      541 aacggttgtt acctgataca gtcttacgac cagtgaaata acatacttta gccattgtat
      601 cttcctcctc atttgatcaa atatatcgga tgtgctagca ccacatacca tattattcta
      661 acagaaaaga ttggacttgg caagaattat tttcattttt attttttaaa gtgtctatac
      721 tcataggtaa atattttaat actacagttt gcgccccatc cataattctt cgttctctaa
      781 gttattacag tcatgaaaac tttggagcag tttatatttt aactgttctc tgctcacttc
      841 tatcgtagat ttcttcatag caaagaacta gatcaaactt aatcaactat tataataaaa
      901 aggattctcg aaagaaaatc ctttttggtt taatctaaga aacttatgct ttgtttgctt
      961 gaaatctaag atttcttcgc agttacatta agctttgttc gctgaaccga atacgtcgat
     1021 acgctcttca acagatgctt ggatagcttt cacgccgtct gccaagaatt tacgtgggtc
     1081 aaagagtttt ttcttatcgt attctgcttc gtttgcttca taagctgctg caaacttacg
     1141 agttgcgttt gcaaatgcga tttggcactc agtgtttacg ttaactttag caacacccaa
     1201 tttgattgct tcttggattt gtgcatccgg aatacctgaa ccaccgtgca atacgattgg
     1261 gaaacctggt acagcttcag tcaatttacg caagtggtca aggtcaagac cttcccagtt
     1321 tgctgggtat ggaccgtgga tgttaccgat acctgctgcc aagaaatcga taccagttgc
     1381 aaccattgct actgcatctt cgattggagc caattcaccg ctacctacga taccatcttc
     1441 ttcaccaccg atagtaccaa cttcagcttc aactgagata cctttagcgt gcgccaattc
     1501 tacaacttct tttgcttttg caaggttttc ttctactgga aggtgtgaac cgtcaaacat
     1561 gatagaagtg taaccaactt cgatacactc aagtgcatct tcgtagtgac cgtggtcaag
     1621 gtgaatagca actggaactg taatgttcat tgattcaatc aagtttgcaa tcaagttacg
     1681 agctactttg taaccaccca tgtacttagc tgcacccata gaagtttgga taagaactgg
     1741 agcttgtttt gcttctgctg cacgcaagat agcttgagtc cactcaaggt tgtttgtgtt
     1801 aaatccacca actgcataac cgttgtcacg agctgcttgg acaaattttt ctgctgaaac
     1861 taatggcatt tcgatagcct cctaatattt tttgaggttg cacctcatac tcttctattc
     1921 taccattttt attttgaaaa tgctagttga aaccgtctag ttttgagaaa actttcacat
     1981 atcttttcat gaaagccata ataatgcctg tttcgtatca ttcttgtaat ttatgaacta
     2041 gcatatatcg tgctctgcac ttatctagac aaagcaaaag gactagcctt agctagtcca
     2101 ctatgcggag agtgggactt gaacccacac gacctaaagc ggtcacagga tccttagtcc
     2161 tgcgcgtctg ccaattccgc catccccgct taacacaaga actagtatat cattaggaac
     2221 tagccttgtc aacacttttt ttgaatttta ttttttattt ttaccttttt ttgccaaaac
     2281 gtattgattt agctgattct tttgcaatat tctactatat ataatgctta aaaccatcta
     2341 gacaatgatg ccatatgggg gtggtatttc gtattttctt ctataaaata ctgacacctt
     2401 tttcgtttct ttatataaaa tatataaaga agattgaatg ataccaatct tcttctataa
     2461 tggttatact tatttttcat tctccatagc tgctgaaaca aaggctgtat acaaaccttc
     2521 agggcgatta ggacgagatt gtagttctgg atggtattgg caggcaacga agaatttatt
     2581 ttctggaatt tcaacaattt ctaccaaacg gttgtctggt gacacgcctg agaaaacaaa
     2641 acctgctttt tcaaattgtt cacggaagtc attattaaac tcataacgat gacgatggcg
     2701 acgttgtaca acttcttgat tatcataagc agctgcagcc ttggagccac gtttgagctt
     2761 gcttggataa agacctaaac ggagagttcc tcctaaatct tccactccaa tttgatcacg
     2821 catgatgtca ataattggat attttgtttc tgggtctaat tcaaagctgt tagcaccttc
     2881 taaaccgaga acgtgacgtg caaattctac acaggtcagc tgcattccca agcagacccc
     2941 tagcattggc acgtcattct cacgagcata acgaatggct tgtattttcc cttctgtacc
     3001 acgttgtcca aagccaccag gaacgataat tccttgtgct tgtcctagac gttcagctac
     3061 gttttctgct gtcaaatcat ttgcattgac ccagtccaat tcgatagcag catcatttgc
     3121 atatcctgag tgttttagag cttcaactac agaaatatag gcatcttgta attctacata
     3181 cttaccaact aaagcgattt ttactttttt ctggagatta agaaccttgt ttaccatcgc
     3241 tgtccactct gtcatatctg ctggaggtac gtcgagcttc aagtgatcgc aaacaatttg
     3301 atccatgttc tgtgcttgca tatttaatgg gatttggtaa agatgttcga catccaagga
     3361 ctcaataact gcttctggag caacatcaca gaattgtgcc agtttatttt taattccttg
     3421 tccagcaggc tgctctgtcc gaattaccaa catgttgggc tgaataccta gaccacgcaa
     3481 ctcttttaca gaatgttgtg ttggcttagt tttcatttcg cctgctgctt tgagataagg
     3541 caagagtgtt gtatggatat acataacatt gtctgaaccg acatctgcct tcatctgacg
     3601 aagcgcttca aggaatggca agctttcaat atcacctact gtaccaccca cttctgtgat
     3661 gatgacgtct gcatctgtag tacgagctgc acgcttgatt ttgtctttca aagcatctgt
     3721 aatgtgtgga ataacttgaa ctgttgcccc taagtattcc cctttacgct ctttacggag
     3781 gacttcgcta taaattttac ctgttgtaac atttgaatat ttgttcaggt taatgtcgat
     3841 gaaacgctca taatgcccaa ggtccaagtc ggtttctgca ccgtcatccg tcacaaaaac
     3901 ttcaccgtgt tgatacggac tcattgttcc cgggtcaatg ttgatgtatg gatcaaattt
     3961 ttggattgtt accttgagac ctcgattctt caataaacga cctaggctgg ctgctacaat
     4021 ccctttgccg atagaggaaa caacgccacc tgttacaaaa atatactttg tcatgcaaac
     4081 tccttttcta cgattcaggc taataggaag caatttgtgg ggatacttcc tgttaacttg
     4141 aagaaaaaac aaaaatagct ccctatcgct agggagctcc gacctcaaaa gaggtgcccg
     4201 ataatatctt atcgtaattc agtagtcttg tcaaattatt tttaagaatc tatggggata
     4261 taattcttat tcgtcgacga cctcttcatc tgaatactcg tcgtcatctt cacctaaatc
     4321 aacatcctct tcatccaatt cgtcatctgg gataatttcg ttgatttctg aatcatagga
     4381 ctcaacttca tctttttcgt catctggatt ttcatcatcg tattctgatg atacactacc
     4441 tggatagtta tcctcatctt ccggatcatc atgtccatag tcaatcgcat catcatcacc
     4501 gtccataaag gcattgacgc gtttcttctt gcgttttggt gcatcttcat catcttcttc
     4561 aagagtgatc acttcttcgt cgatctcatc gattgcgtac catgaacgta agccccattt
     4621 gttttcgcca agcggaatga agcttccatc cacattcaaa tcagaataga aggttgggag
     4681 ggctgcgcga atctcgctgt ttgatttttc aaggtagttt tgaatttcat tgaccaaatc
     4741 gttgaaatac atttcgttgt cacgtccacg ttcttccaaa atagcacggg ctacttcaat
     4801 catggaaagt tcgctttttt cttgtcctgc aaatacgtta agttccaagg ctgttctcct
     4861 tttttgtctt tcccttctat tttacgctaa aaaaggcaaa tagtcaaaga taatctttat
     4921 cgtttttctg taaaaaaata cctgcagagc tcagtgctac aggtattttg tttactatac
     4981 ttgttcttct aaacttggaa gggagatggt ccgtttgacc ccatccttat ctacaagtcg
     5041 aacaactttg cttgctgcat catattcgaa agcttcaaaa gaaactttgt aacggtctga
     5101 gagtgtgacc agactttgtt caaaggtttt gaagtccatg ccaaactctt ctgctcttcg
     5161 ttttaattct tctgtaaact cgtcaatttc ctcggtttct tcagaaataa tttcttcatc
     5221 actttcttcc gtctctgttt cttctgttga ttcagatggt gtatttggtg taaatactcc
     5281 ccagccatcg tctgaaactg ggcgttcttg tggattagtc atgtagtatt taaccgttgc
     5341 taggaaatct tctaaactat acccttcagg acttcttgca ctaccttggt caaaccattt
     5401 aaaggataca tagtgatagt gatcttgatg cggaatcacc agagtcccgt tgcgtacttc
     5461 ggttgcatag ccagcattgt aagtcagtgc ttcgaaagga acgactttct tcgcttccac
     5521 tcggttataa atttcctctg ctgtttcttt ggcaacttgt ggctgttctg gcgttgttgt
     5581 ctcgcctgca cttgctcctg tttcagttgg tgcaggtgtt gacggtaaac tagaattaga
     5641 tttctcacgt tgagccacca tagcctctgc tgggatcagc tcgcttaagt aattcaaatc
     5701 actcttgtta atccgacggt aaccatctgt aatcacacgt gggatgcgat agttatccgt
     5761 acctattttc actttgggat ctaataggtc cgctgggtca aaaatatagc catcttttgt
     5821 tgtatatttt cctgctgcct tagcttctgc caattcttct gctgagtgaa caatttgcca
     5881 atttttcaaa cctgcacgtt tctcaattgg tgtgacattt ttcgccaatt cttcagcgtc
     5941 tcgtttatca agaagagttt gtacagcttc caattcttct ttagataaat ctttcttgga
     6001 aattgaacgt agagaacctc ctgtcgctct tggtagactg aacgcttgac taaagacaaa
     6061 acttgctgga tctagtacgt cttccgccga gaagatatag ccatcatttg tcgcaaattt
     6121 cccttttgca cgcgcatcca taacttcttc tgctgtgtga atgatttgcc aatttttcaa
     6181 gcctaagcgg tttgctttag gtgttgcatt tagaattggg ggaacaagat ccgttgcttc
     6241 tccatcacca tgtccacttg tagtccaagc agatggacga atttcaggat gttgcatgat
     6301 atagcggatc gttgcaattt gttccttact caaccatgtg taaggtagga cgtggatgtg
     6361 atcaatgtgt ggaataataa aggattgtcc agtatcatag gtcgcattcg ctcccttcat
     6421 cggaatttga tccataccga ccaacatggc tggcttaaga tctccttctg caagcggtgc
     6481 aatatcaaag atatactgct tatctttttc tgccaaagtc aactcagcaa aagagatctt
     6541 catcaaatct agttcgtcac gaccatacgt ataagttttt ccacctactt ccatttcata
     6601 aacaactttt ccagccaatt tacgtttagc cgttacttgt ttatattcaa atgcagggcg
     6661 gttttcttct tgaattggtt ttacaggttt tgaatcattc ccttgacttg gtgttgtggg
     6721 tttagcatcg tttcctactt gttcagatgt tttcggtggc acttgtttgc ccgccttctc
     6781 attaacccat tcctctactt gctttatttc aaaatcttcg agttcgccaa aaccaacata
     6841 atggaaatga tctccatgac tagctatcaa cccttgatca tcaactgagg taatagagtc
     6901 tttgctaaag gtgtatccat cacttgtaaa atatgcctta ccatctaacc cttttccgta
     6961 agcaggaatt tgacgtccct tataggaaac cactttccct gttgactgct tcggttgtgt
     7021 tggtaatact ggttgtgtag gcgataccgg ttgcgttgta acagtgccga ttggtgttac
     7081 tggacgattt ggttgtgtta gtgtatgttg ggagtgactt gcttgtgcgc cagaagaaac
     7141 acctgctcca tttacaccaa tcattcgaga aatcttctct tctagaggag acatctggct
     7201 gtatggaata aagtggtaat gatcgccgtg tggaacgatg acacctgctg cggttttctt
     7261 cgtaatcgtt cttgggtcaa ataccaatcc atccgcttct acatggcgtt gagatagggg
     7321 tgttgcatcc aattgagcca ataaactagc caaatcctga ccttgccccg cactaattgt
     7381 ggtctgttga gctctattat ttctatcgcc atattgacta ccactagccg agctcacact
     7441 tcctttttga ttccagtaat cttgagcagc cttcaattcg gcagctgata aatctttttt
     7501 aggaataaag tggaaatggt cgccgtgcgg aacgataaaa ccatctcccg tgtcttcaat
     7561 aacatctgtc ggattaaaga catagccatc gtctgttctg tagcggccat ccttactatt
     7621 tcctgctgcc tgatttttgc tgtcagcaga agagattcct ttttgccgct ccacttcttc
     7681 tttgctacga acatttttat gtttgctagg gtcttttaga tagagatagt atttcccatc
     7741 aacttttatg acgtagccat cctggacttc attaatgatg tgactgtctt gtaagacata
     7801 gtttgggtcc ttcatgacca actcttcact aaaaatcgca tcaaagggga ctttaccatt
     7861 atagtagtgg aaatggtcac cgtgtgatgt cacatagcct tgatcagtaa ttttaacgac
     7921 tatctgctca gcgtcaatat tttccttggc agaaacttga tcaggtgtta attgttcagc
     7981 aacggtagtt tgcacacttt gactatcttc aatataggac acacgatttt tttgctcttc
     8041 catcgcttgg aaacgtccaa gctgatagct acagagactc atccctaaaa caagggcggc
     8101 tacggagcca acaactgctt ttttcttcat tttttctcct ttcggtcgat agaattcggc
     8161 cttttatttt aactcttgag ctaatgtttc taagacttgc tctagatttt ctagataggt
     8221 taaattattt tctggatctg cttcaagagg atccaacact tttaaatcta cacctgtcga
     8281 actcgccaat gccttggcaa gcttatccga ggcacctttt tctgtaaaaa ttgtttgcac
     8341 attgtaggtg tcaacaaatt ctttaatttc cgccaactgt ctaggacttg gttcttcctc
     8401 tgaaactcct gcgatcccca actgcttcaa cccgaagcgt tgcgcagtgt aagagaaggc
     8461 tgtgtgttga gtgacaaatg tttttgatgt ggcttttaaa aagattgggc tatacttgtc
     8521 tgccaacttt tgcgcctttc cctctaaagt tgcggcattt tgacgataat aatccgcatt
     8581 cttaggatca ctttctgcta aaagttcacc aatcgcaaca gcttcctgac caaccaaaac
     8641 tggatccagc catgtgtggg ggtcatataa actagcttca tcaatccctt gaccagcttc
     8701 catgtcttct aaccctggaa cctttgtcag cggtaaattt gtcgaagcct ccaaaacctt
     8761 gacagatgaa ccttgcaaat taggttccaa acgtcctgcc caggattcca aaattcgcga
     8821 atgatagata aaaacatctg catcgtatat ggcctttatg tccgcagccg atggttcata
     8881 agagtgtatg ccctgtcttg agccaatcat tcgaacatca tttttattcc ctgacacttc
     8941 cttaaccagt gaataaatcg ggtaaaatga ggtgactatt ttcattcctg gctgtacttg
     9001 actcgcttcc ttctgagccg agcaagctgc taaaacaaaa ccgaataagg caacaaaaca
     9061 gcctcttatc actttcttta acataacttc ctcctttgtt aaccagttaa ctataacaca
     9121 agaatattaa ccagtcaagt attttttaaa aaaagataag actttctttt tataagccag
     9181 tcttac
//
LOCUS       DBNFCK010000072         9111 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.072, whole genome shotgun sequence.
ACCESSION   DBNFCK010000072 DBNFCK010000000
VERSION     DBNFCK010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9111
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.072"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            72..1619
                     /gene="cls"
                     /locus_tag="AC1PIW_001339"
     CDS             72..1619
                     /gene="cls"
                     /locus_tag="AC1PIW_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HIP6606271.1"
                     /translation="MEKLWKIIYSRTFIVISLIILTIFLILWLVGSAATYMPALLIVL
                     QIFSIFVAISIINRPMNTSFKLTWIIFVIGIPIFGALFYFILQSNIETKRYRKNFHKQ
                     AQRLREYSKTSEKVMKALEQEDKEQLKLAHFMSEYVGYPIHTNTDAVYFSTGQEKFEA
                     LLEELKKAEQYIFMEYFIVDMGYMWDSILDILKEKAAQGVEVRFMYDGMNSLTNLPYN
                     YYKTLRKYGIKAKVFSQIIPALSTVQNNRDHRKIAIIDGKVAFTGGINIADEYINKKV
                     RFGYWKDAAIMIRGEAVANFTLMFLQMWNYNEKTETDDLKYLKTHKDLETETVDAEGF
                     FLPYGENPFDGDEVAKRVCLDMIQSATDYIYIMTPYLVLDDEMIDNLTYAAKRGVEVR
                     ILLPHIYDKKSAYLAARTDFPTYLEAGIEIFEFQPGFVHSKVVLVDDKKATVGTVNMD
                     FRSFYLNFECGLYIYNHSEVLAAIKDDFTHSFKKSQQINLLTFHKTYSWYKRLGGALL
                     RIISPLL"
     gene            1775..3988
                     /gene="recJ"
                     /locus_tag="AC1PIW_001340"
     CDS             1775..3988
                     /gene="recJ"
                     /locus_tag="AC1PIW_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIP6606272.1"
                     /translation="MIKPNYDWQLLTGFSDEQFIKIAKKEGVDPVAAKLLYERGIHSA
                     EELHTFLQPSLEDLHDPYLLHDMDKAVERIRRAIENYEHILIYGDYDADGMTSASILK
                     ETLEEMGAEVQVYLPNRFTDGYGPNQSVYKYFIEQQGISLIVTVDNGVAGHEAIAYAQ
                     EMGVDVVVTDHHSMQETLPNAYAIVHPEHPEGNYPFKHLAGCGVAFKLACALLETVHA
                     DLLDLVAIGTIADMVSLTDENRVMVKYGLSLLKQTERAGLQELIKIAGIDIDSIDEET
                     VGFQLAPRLNALGRLDDPNPAIELLTGFDDEEAHQIALMIDSKNVERKDVVQAIYDEA
                     KTMLRHDRPVQVLAKEGWNPGVLGIVAGRLLEELQQPVIVLSIEDGKAKGSARSVEAV
                     DIFKALKDHQDLFIAFGGHAGAAGMTLEVDKLEELALTLTDYIIENKLDLSSKSSLVL
                     DEELDLEELTLDTLKSFEKLAPYGMDNKKPVFYIRDFQVESARTMGQNNAHLKLRITK
                     GAAGFDVLAFGKGNLALEFSQAKGLELAVTLSVNQWNGNTSLQLMLVDARVNGVQLYN
                     IRGKQHPIPAGVPILDIENPVADNKAIVLANLPEDLSSLRSYFQEREFEAIYFKNEIA
                     KPYYLDGYGSREQFAKLYKTIYQFEEFDVRYKLKDLAVYLKIKDSLLVKMIQIFQELE
                     FVTIADGVMRVNKEAQKREISESQIYQELKETVIQQELMALGTVQEIYEWLCGRV"
     gene            3967..4863
                     /locus_tag="AC1PIW_001341"
     CDS             3967..4863
                     /locus_tag="AC1PIW_001341"
                     /inference="COORDINATES: protein motif:HMM:NF022755.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HIP6606273.1"
                     /translation="MAMWSRLMTVEALVVGVIGTLLYTYWFIRQKGLLKNLEQITEIK
                     HALIELRRVGWSERAIKKVFLKQLLPLKQEDIPAFVQNFIKEATIFASTSFYQLIRVD
                     SRSLSQEKATALLEQCMNMLDFPEELSHGILPELLQKMDVNCPPHHPFWRYFAKLVDK
                     AFPVRELEVKRPLNRQVHQLRYLISYQQTFWVRQQFGKGKTDWQALIAYVRSLPRWSY
                     RLRESARLHNKQLFGKKNQKTLPVNMKILIHFHSEFILNQDGQFALILEERPHVNGVV
                     NGASFNYARANNKREWERTRLV"
     gene            5296..5808
                     /locus_tag="AC1PIW_001342"
     CDS             5296..5808
                     /locus_tag="AC1PIW_001342"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HIP6606274.1"
                     /translation="MNLKDYIATIPNYPKEGIEFRDISPLMADGNAYSYAVREIVQYA
                     TDKQIDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGIVAGCAFLIELDDLKGREAIG
                     DYDYKVLMHY"
     gene            5912..6853
                     /gene="metA"
                     /locus_tag="AC1PIW_001343"
     CDS             5912..6853
                     /gene="metA"
                     /locus_tag="AC1PIW_001343"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase MetA"
                     /protein_id="HIP6606275.1"
                     /translation="MPIKIEKSLPAVEILRKENIFVMDSDRASHQDIRPIKILILNLM
                     PQKIVTETQLLRLLANTPLQLEVEFLYMASHESKNTHSGHLKQFYKTFEQVRDSYFDG
                     LIVTGAPVENLPFEEVDYWHELTQVFDWSKTHVYSTLHICWGAQAGLYARYGVPKHSM
                     SRKLSGVYCQEVTNPTSPLMRGFDDEFRSPHSRYTEVREADIAHLSGLTILSQGPEVG
                     LSILASKDLRGIYSFGHLEYDRDTLAKEYQRDCIAGKNPHLPENYFKGDDPTSKPSLS
                     WNLPAAQFFTNWVNYAVYQETPYDWHIFEQSAQSASL"
     gene            6863..7537
                     /locus_tag="AC1PIW_001344"
     CDS             6863..7537
                     /locus_tag="AC1PIW_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HIP6606276.1"
                     /translation="MNYSQQFRQGHLVLPAAILFHYQELFPSADDFLIWQFFLYQNSS
                     AIESLAPSEIAQATGKTVAQVNQAIENLQDAGLLEFKTISIAGEIEMIFDALPAFEKL
                     DVLLTPKQAVEIVQPENDLKTLVGDFERELGRFLSPFEIEDLQKTIEDDKTSIDLVRA
                     ALKEAVFNNKTNWKYIQAILRNWRREGITTVVQVEAKNAEREIQTPKNVTVSSDFLDA
                     MDLWKD"
     gene            7539..8222
                     /locus_tag="AC1PIW_001345"
     CDS             7539..8222
                     /locus_tag="AC1PIW_001345"
                     /EC_number="2.1.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0160105 - tRNA
                     (adenine(22)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase"
                     /protein_id="HIP6606277.1"
                     /translation="METKLSKRLETVASFVPQGARLVDVGSDHAYLPLFLVEQGRIDF
                     AIAGEVVQGPYQSALQNVEQAGQTEKILVRLANGLGAFEKSDQMTAVTIAGMGGRLIA
                     EILEAGKEKLATIERLILQPNNREDDVRRWLEKNGFRLVAEKILEENGKLYEVLVAES
                     GQMRLSEVERRFGPYLLEETSPVFVKKWRRELEKLSFALEQVPSERQGDRFAISQKIQ
                     QIQEVVHVS"
     gene            8212..9006
                     /locus_tag="AC1PIW_001346"
     CDS             8212..9006
                     /locus_tag="AC1PIW_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HIP6606278.1"
                     /translation="MLASQVIERYQAFCPHSLAMEGDVKGLQIGTLNKEIQRVMVALD
                     IRETTVAEAIDKKVDLIIVKHAPIFRPINDLVADNPQTKMYLDLIKHDIAVYVSHTDI
                     DVVEGGLNDWFCELLEIQDTTYLIETAEGQGLGRVGTIAEQTLEELALKVKSVFDLDA
                     VRLVTYGHQAKQYVSRVAICGGSGGSYYHEALAKGADVYITGDIYYHTGQDMLTQGLL
                     AIDPGHHIEVLFIKKIAELLEIWKAKEGWDVEILPSTVSTNPFRHL"
BASE COUNT         2732 a         1638 c         2074 g         2667 t
ORIGIN      
        1 gagactattt attttgagta ggataatgaa ctaaaatctg ttataatggt aaaaaactaa
       61 gggagagatg tatggaaaaa ctttggaaaa ttatatatag tcggacattt attgtcatta
      121 gtttgattat tctaaccatt ttccttattc tttggttggt aggttcggca gcaacctata
      181 tgcctgcttt attgattgtt ttgcaaattt tttcgatttt tgtagctatt tcaatcatca
      241 atagaccgat gaataccagt tttaagttaa cctggattat ttttgtcatt ggtattccga
      301 tttttggtgc cctattctat tttattttgc agtccaacat tgaaaccaaa cgttatcgta
      361 aaaattttca taagcaagca caaagacttc gggagtatag taagacgtct gaaaaggtca
      421 tgaaggcgtt ggagcaggaa gataaggagc aactgaaatt agctcacttc atgagtgagt
      481 acgttggcta cccaattcat accaatacgg atgccgtcta tttttcaaca ggtcaggaaa
      541 aatttgaagc tctactagaa gaattgaaaa aggcagagca gtatattttc atggagtact
      601 ttattgttga tatgggatat atgtgggatt ctatccttga cattttgaag gaaaaggcag
      661 cgcaaggggt ggaagttcgt tttatgtatg atgggatgaa ttcactgacc aatcttccct
      721 ataattacta taaaaccttg agaaaatatg gaataaaagc caaagtattt tctcaaatta
      781 tcccagcctt atctacggtg caaaataacc gtgaccatag aaaaattgcg attattgatg
      841 gaaaggttgc ctttaccggc ggcattaata tcgcagatga atacatcaac aagaaagtgc
      901 gttttggtta ctggaaagac gcagcgatta tgattagggg agaagctgtt gcaaacttta
      961 cgttgatgtt ccttcaaatg tggaattaca atgaaaagac agagacagat gacctaaaat
     1021 acctcaaaac gcataaagat ttagagacgg aaaccgttga tgccgaaggt ttcttccttc
     1081 cgtatggcga gaatccattt gatggagatg aggtagccaa acgagtctgc ctagacatga
     1141 ttcagtcggc aacggattat atttatatca tgacacccta cctagtgttg gatgatgaaa
     1201 tgattgataa tttgacttat gctgcgaagc gtggagtcga ggttcgcatt ctgctaccgc
     1261 atatctacga taaaaaatct gcctatttgg ctgctcgtac tgattttccg acttacttgg
     1321 aagcaggaat tgaaatcttt gaattccagc ctggatttgt ccattccaaa gttgttttgg
     1381 tcgatgataa gaaagctact gttggtacgg tgaatatgga ttttcgctct ttttatctta
     1441 attttgaatg cggtctctat atttacaatc actcggaagt tttggcagct attaaggatg
     1501 actttactca ttccttcaaa aaatcacagc aaattaacct tctaaccttc cataaaacgt
     1561 actcttggta taagcgtttg ggaggagccc tactgcgaat tatttcgccg ttattataaa
     1621 ttgtaatatt cgttcttcat ggagcaaggg atcgacattc tgtttcggtc ttaccttgct
     1681 ccatttttct tggaaaatga tataatagaa agatagttta tactcggaaa atttacaggc
     1741 tacttgtgat tttgagtatt aaggagtaaa aaacgtgata aagcccaatt atgactggca
     1801 attattgacc ggtttttctg atgaacaatt tatcaaaatt gctaaaaaag aaggagtgga
     1861 cccggtagcg gctaagttgc tgtatgaacg tggcatccat tcggcggaag aattgcatac
     1921 tttcttacaa ccaagcttag aagaccttca cgatccctat ttactacatg atatggacaa
     1981 ggcggtagag cggattcgcc gagcaatcga aaattacgaa cacattttga tttatggaga
     2041 ttatgacgca gacggtatga cctcggcatc tattctgaaa gaaactctgg aggaaatggg
     2101 ggcagaagtc caagtttatc ttcccaaccg ttttacagat ggctatgggc cgaatcagtc
     2161 agtttacaag tattttatcg agcaacaggg gatttctctt attgtgacgg tggacaatgg
     2221 cgtagcgggt catgaagcga ttgcctatgc ccaggaaatg ggtgtcgatg ttgtcgtgac
     2281 agaccaccat tccatgcaag aaacccttcc caatgcctat gcgattgtac atccagagca
     2341 tccagaagga aattatccct tcaaacattt ggcgggctgc ggcgtggctt ttaaattggc
     2401 ctgtgctctt cttgaaactg ttcatgcaga cttgctggat ttggtcgcaa ttggcaccat
     2461 cgctgatatg gttagcttga ccgatgagaa tcgtgtcatg gtcaaatatg gtctctcgct
     2521 tctcaaacag actgagcgag caggtttgca agagttgata aagatagctg gtattgacat
     2581 agacagtatt gatgaagaaa cagttggttt ccaacttgct ccgaggttga atgcccttgg
     2641 tcgactcgat gatccaaatc cagccatcga attactgact ggttttgatg acgaagaggc
     2701 ccatcaaatt gccctcatga ttgacagtaa aaatgtagaa cgtaaagacg tcgtccaagc
     2761 aatttacgat gaagcaaaaa ccatgctccg tcatgataga cctgttcaag ttctggctaa
     2821 agaaggttgg aacccaggtg ttttagggat tgtagcagga cgcctactag aagaactgca
     2881 gcaacctgtt atcgtcttgt ctattgaaga tggtaaggca aagggttcgg ctcgttctgt
     2941 tgaagcagtt gacattttca aggcacttaa agatcatcag gacctattta tcgcctttgg
     3001 tggtcatgct ggtgcagcag gtatgacctt ggaagttgac aaacttgaag agcttgctct
     3061 gaccttgaca gactacatta tcgaaaataa attggattta tccagcaagt cttcgctggt
     3121 cttagatgaa gaattggatt tagaggaatt aaccctagat actctaaaat ctttcgagaa
     3181 gcttgcgcct tacggcatgg ataataaaaa gcctgtattt tacatcagag actttcaggt
     3241 ggaatcagca agaaccatgg ggcagaataa tgcccatttg aaactacgca ttactaaagg
     3301 tgctgctgga tttgatgttc ttgcttttgg taagggcaat ctagccttgg aattttcaca
     3361 ggccaagggc ttagaactag ctgtcacctt gtctgtcaat caatggaatg gcaataccag
     3421 tcttcagctc atgttggttg atgctcgggt caatggtgtt caactttata atattcgtgg
     3481 aaaacaacat cctattcctg caggggtccc aattttggac attgaaaatc cagtagcaga
     3541 taacaaagca attgtcttgg ctaacttgcc tgaggacctc agtagtctgc gtagctattt
     3601 tcaggaaaga gaatttgaag ccatttattt taaaaacgaa attgccaaac cgtactatct
     3661 agatggttat ggtagccgag aacaattcgc taaattgtat aagactattt accaatttga
     3721 agagtttgat gttcgctata aactgaaaga tttggctgtt tacttaaaaa ttaaggacag
     3781 tctattggtt aaaatgattc agattttcca agaattagaa tttgtcacga ttgcggatgg
     3841 ggtcatgcgg gtcaacaaag aggcacagaa gcgagagatt tcagaaagtc aaatatatca
     3901 agaattgaaa gaaacagtta tccaacaaga gttgatggcg cttggcaccg ttcaggaaat
     3961 ttatgaatgg ctatgtggtc gcgtttgatg acagtagaag ctctggttgt tggagtgatt
     4021 ggaacgcttc tctacactta ttggtttatc aggcaaaaag gtttgttgaa aaatcttgaa
     4081 cagataaccg aaataaagca cgcactgatt gaattacggc gagtaggatg gtcagaaaga
     4141 gccatcaaaa aggtttttct gaaacaactg ttgcctctaa aacaagaaga catcccagca
     4201 tttgttcaaa atttcataaa agaagctact atttttgcct caactagctt ttatcagttg
     4261 ataagagttg acagtcgctc tttaagccaa gagaaagcga cggccttatt ggagcaatgt
     4321 atgaatatgt tagattttcc tgaggaatta agtcatggga tattacctga acttttgcag
     4381 aagatggatg tgaattgtcc tccacaccat ccgttttggc ggtactttgc taagctagtt
     4441 gataaggctt ttccagttcg ggaattagaa gtcaaacggc cgttaaacag acaggtccat
     4501 cagctacgct accttatttc ctatcagcag actttttggg tgcgtcagca gtttggaaaa
     4561 ggaaaaacag attggcaggc tctgatagcc tatgtacgta gcctacctcg gtggtcttat
     4621 cgcttacgag aatctgcccg cctacacaat aaacaactgt tcggcaagaa aaatcagaag
     4681 accttgccag tcaatatgaa gattttgatt cattttcaca gcgaatttat cctaaatcaa
     4741 gatggacaat ttgccttgat tttagaagaa cgaccgcatg tcaatggcgt ggtaaatggg
     4801 gcttccttta attatgccag agctaataat aagcgggagt gggaaagaac tcgactggtc
     4861 taaaaagagt tcgtcttccc acccccgcac agttgattag gttggatttg gagtgtaaaa
     4921 cacgaacaaa tccgccaatc aaccactgca ctgagatgtt gacacgaact ctaagaagtg
     4981 gcgctgagct ttttgccctg cctctccagt tggcaggcag gatcccattt ttcggaaaga
     5041 attgctacgc tcaaagatgg gagtctattt atctcctact cgctttcgtc gctaccaaaa
     5101 aggaagaaat gaggttggct gggagcagtc ttactttaat cagcaaggaa gcttttccta
     5161 tggtggacat agtagagcag cttgtgtggc gaatttgagg agacgatttg ctaaagatat
     5221 atgtctgaaa tgtaccaaaa aacaatttca tggtataatg aagagtaaaa attattttta
     5281 aaaagagaga gacaaatgaa cttaaaagat tacatcgcaa caattcctaa ctatccaaaa
     5341 gaaggtatcg aattccgtga cattagccct ttgatggcag atggaaatgc ttatagctat
     5401 gcagtacgtg aaatcgttca gtatgcgaca gataagcaaa tcgatatgat tgttgggccg
     5461 gaggcgcgtg gtttcatcgt tggatgtcca gttgcctttg aattgggaat cggttttgca
     5521 ccagttcgta agccaggtaa gttaccacgt gaggttatct cagctgatta tgaaaaagaa
     5581 tatggtgtag atacattgac gatgcatgca gacgcaatca aaccaggaca acgtgtcttg
     5641 attgtcgatg acttgttggc gactggtggt actgtaaaag caacgatcga aatgattgaa
     5701 aagcttggcg gtatcgttgc tggctgtgcc ttcttgattg agctagatga tttgaaaggc
     5761 cgcgaagcaa tcggtgatta cgactacaag gttttgatgc actattaatc taaggttata
     5821 gccaaaactt ataactggtc ctatttttat tctaattgaa ttgcatagca acctgtctta
     5881 taataaatat aagaaaattg ggaggttgct tatgccaatt aaaattgaaa aatcattacc
     5941 agcagttgaa attttgagaa aagagaatat ttttgtgatg gatagtgacc gagccagtca
     6001 tcaggacatt cgtcctatca aaatccttat tctcaatctc atgccacaga agatagtgac
     6061 ggagactcag ttgctccgtc ttttggctaa cactccttta cagctagagg ttgaatttct
     6121 ctacatggct agtcacgagt ccaaaaatac acactcgggc catttgaagc agttttataa
     6181 gacttttgag caggtcaggg atagctattt tgatgggctc atcgtgacag gagcaccagt
     6241 tgaaaattta ccttttgagg aagttgatta ctggcatgaa cttactcaag tttttgattg
     6301 gtcaaaaaca catgtttatt caacattgca catttgttgg ggtgcacagg caggtttgta
     6361 tgcacgctat ggtgtaccca aacacagtat gtctcgaaaa ctatcagggg tatattgtca
     6421 agaagtgact aatccaacga gccctcttat gcgtggattt gacgatgaat ttcgttcacc
     6481 acattcccgt tatacggaag taagagaagc agatattgcc catctgagtg ggttgacgat
     6541 tttatcacaa ggtccagaag ttggcctatc cattctagcc agcaaggatt taagaggaat
     6601 ttatagtttt ggacatttag agtatgatag ggatacacta gctaaggaat accaacgtga
     6661 ttgtatagct ggaaaaaatc cccatttacc ggaaaattat ttcaaaggag atgaccctac
     6721 aagcaaacca tctctctctt ggaatttacc agcagcacaa ttctttacca actgggtgaa
     6781 ttatgctgtc tatcaagaaa ctccatacga ctggcatatc tttgaacaat cagcccagtc
     6841 agctagtctt taaagaggaa gtatgaacta ttcacagcaa tttcgtcagg gacatttggt
     6901 cctgccagca gcaatattat tccactatca agagcttttt ccgtcggcag atgatttttt
     6961 gatttggcag tttttcttat atcaaaattc ttcagctatc gaatcattag cgcctagtga
     7021 gattgctcaa gctactggta aaacagttgc tcaggtcaat caagctattg aaaatctcca
     7081 agatgctgga ctcttagaat ttaagacaat cagtattgct ggtgaaattg aaatgatatt
     7141 cgatgcgctt cctgcttttg aaaaattgga tgttctgtta actccaaaac aggcagttga
     7201 gattgtgcaa ccagagaatg atttgaagac cttggtgggt gattttgaac gtgaattggg
     7261 ccgcttttta tcgccgtttg aaattgaaga tttgcagaag actattgagg atgacaaaac
     7321 ttcgattgat ttggttcgtg cagccttgaa agaagctgtt tttaacaaca aaacaaattg
     7381 gaaatacatc caagctattc tacgaaactg gcgccgtgag ggaattacta cagttgtgca
     7441 agtagaagcg aaaaatgcag aaagagaaat ccagactcct aagaatgtga ctgtttcaag
     7501 tgactttctt gatgcaatgg atctctggaa agattgatat ggaaacaaaa ttatctaaaa
     7561 ggctggaaac agtcgcaagt tttgtcccac aaggggcacg tctagttgat gtagggagtg
     7621 accatgctta tctccctctt tttttggtag agcaggggcg tattgatttt gccattgcgg
     7681 gggaagtcgt tcaaggtccc tatcagtcag cgctccagaa tgtagagcag gcgggacaaa
     7741 cagagaaaat tctagtccgt ttggcaaatg gcttaggcgc gtttgagaag tctgaccaga
     7801 tgactgccgt gaccatagca ggtatggggg gacgcttgat tgcggaaatc ttagaagcag
     7861 gaaaagaaaa gcttgcgacc attgaacgtt tgatactcca gccaaataat cgtgaagatg
     7921 atgtgcgtcg atggttggaa aaaaatggtt ttagattggt tgctgaaaag attctggaag
     7981 aaaatggcaa gttatatgaa gtcctcgttg cagaatcagg acagatgaga ttgtcagaag
     8041 ttgaacgtcg ttttggtccc tatctactag aggagacctc gccagttttc gtcaaaaagt
     8101 ggagaagaga acttgaaaaa ttaagttttg ctttggaaca agttccatct gaacgccaag
     8161 gtgaccgctt tgccatttcc caaaagatcc aacaaattca ggaggtagtc catgttagct
     8221 agtcaggtta ttgagcgcta tcaagccttt tgtccccatt ctttggctat ggaaggcgat
     8281 gtcaaagggc tgcaaatcgg tacccttaat aaagagattc aacgtgtcat ggtggctctg
     8341 gatattcgtg agacaacagt ggcagaggcg attgataaaa aggtagattt gattatagtc
     8401 aagcacgctc cgatttttcg tccaataaat gatttggtgg ctgacaatcc gcaaactaaa
     8461 atgtatcttg atttgattaa gcatgatatt gcagtctatg ttagtcatac agatattgat
     8521 gtggttgagg gtggtctcaa cgactggttc tgtgaactac tagaaattca agatactacc
     8581 tacttgattg aaactgccga ggggcagggg cttggccgtg tggggacaat agcagagcag
     8641 actttggaag aattggcttt gaaagtcaag tctgtctttg atctagatgc tgtccgtcta
     8701 gtgacctatg ggcaccaagc caagcagtat gtcagccgtg tagccatctg tggaggcagt
     8761 ggtgggtctt actaccatga ggctcttgct aagggggcag atgtttacat cacgggcgat
     8821 atttactacc atactgggca ggacatgctg actcagggct tgctggctat tgatccaggc
     8881 catcatatcg aagtattatt tatcaaaaaa attgctgaat tgttggaaat atggaaggca
     8941 aaagaaggtt gggatgtcga aattctacct tcaactgttt caaccaatcc gtttagacat
     9001 ttgtgataaa ttaatcagca cttgattgtg gaaagaccaa gacgacattt aatgtagttt
     9061 taggtctttt attttttaga tattttctta tttatttaag tgaaaaagct t
//
LOCUS       DBNFCK010000073         8954 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.073, whole genome shotgun sequence.
ACCESSION   DBNFCK010000073 DBNFCK010000000
VERSION     DBNFCK010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8954
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.073"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..755
                     /locus_tag="AC1PIW_001347"
     CDS             <1..755
                     /locus_tag="AC1PIW_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HIP6606279.1"
                     /translation="DQLTNSFDQEKKTLASEEVKERIDLAQAFNESLNNVVSEDPYTK
                     SRHEAGRVEYARMLELHEKIGYVEIPKIEVKLPVYAGTSETVLQKGVGHLEGTSLPIG
                     GSDTHTVLTAHTGLPKARLFTDLTKVKIGDTFYIHNIVETLAYEVDQIVVAEPTQFEE
                     LLVKPGQDYATLLTCTPYMVNTHRLLVRGHRIPYVAEEHLKASENAEKRILIRYLLYA
                     LGMFVGILLIFLLTKRRKNKKKVTSNDETFTT"
     gene            733..1590
                     /locus_tag="AC1PIW_001348"
     CDS             733..1590
                     /locus_tag="AC1PIW_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HIP6606280.1"
                     /translation="MMKRSQLRKGISKQSILLKLLFLFGLVVTLYPWISQYYYRYDSD
                     YKIQSFYEQAQQLPSEEVLKRLEHARAYNQTLEPHKLQDPYTEEEKAGVVEYARMLEV
                     KEKIGFVEIPKINERIPIYAGTTEEVLQKGAGHLEGSSLPVGGESTHTVITAHRGLPN
                     ASLFTNLDHLKVGDQFYIHNIAEVLAYEVDQILVVEPNNFDPVIVQNGKDYATLLTCT
                     PYMINSHRLLVRGHRVPYEPKVAIKQKPAFFLDMLTLSYLVALLIIVVIVIILYLLRR
                     KKYQKGEMK"
     gene            1587..2411
                     /locus_tag="AC1PIW_001349"
     CDS             1587..2411
                     /locus_tag="AC1PIW_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HIP6606281.1"
                     /translation="MMKRIGYVFILVGLLLPLVLLTNMSVQEFQVFQQYQYYRRNSKF
                     FSTEQLEEIKAYGEQVSGGDLPTVDPFAEKNDSNSTDVLSSELMEGAIGYLSIPKIEI
                     RQPIYVGATSEHLNDGVASVIGTALPIGGIGRRSVIAGHRSWYNDLRFFRLSELREGD
                     KIFIEVGGRTLTYLVKNTEVIKATDWQKLLPVESQDMVTLLTCDPLVPPFDYRLLVNA
                     YRESDVIEEADEVSQTSQREIKQFQQYSFSLVFYLTIFGWVLLLYILYRFIRTYIW"
     gene            complement(2933..5374)
                     /gene="sntA"
                     /locus_tag="AC1PIW_001350"
     CDS             complement(2933..5374)
                     /gene="sntA"
                     /locus_tag="AC1PIW_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein SntA"
                     /protein_id="HIP6606282.1"
                     /translation="MNFRFSKCAVALTLALLAASNPKLAQAEEILNTTPASSTEASQA
                     VPVESDTTEEADNTESPVPATTEAENPSSSETAETSDPTSETTDTTTSEARTVTPAAT
                     ETSQPVEGQTVDVRILATTDLHTNLVNYDYYQDKPVETLGLAKTAVLIEEAKKENPNV
                     VLVDNGDTIQGTPLGNYKSIVDPIEEGEQHPMYAALETLGFDVGTLGNHEFNYGLAYL
                     EKVIRTANMPLVNANVLDPTTKDFLYTPYTIVKKTFTDTEGKKVTLNVGVTGIVPPQI
                     LNWDKAYLEGKVIVRDAVEAVRDIIPTMRENGADIVLVLSHSGIGDDQYEVGEENVGY
                     QIASLSGVDAVITGHSHAEFPGTAEKPSFYAKYSGVDDTNGKINGTPVTMAGKYGDHL
                     GVIDLNLVFKDGKWTTTSSKAAIRKIDTKSSVADGRIIDLAKEAHNETIKYVRQQVGE
                     TTAPINSFFALVQDDPSVQIVNNAQIWYAKQQLAGTSEANLPILSAAAPFKAGTRGDA
                     SAYTDIPAGPIAIKNVADLYLYDNVVAILKVNGAQLKEWLEMSAGQFNQVDLSSTEPQ
                     NLVNTDFRTYNFDVIDGVTYQYDITQPNKYDRDGKIVNETASRVRNLQYNGQDVTADQ
                     EFIVVTNNYRANGTFPGVREASINRLLNLENRQAIINYIIAEKVINPTADNNWTFTDS
                     IKGLDLRFLTADRAKSLVTDQECIVYLQASTASEGFGEFKFVYTESKVVTPDEQQSDQ
                     GNTGQDIVLESGQRITLPAVNPPAPAPQHKLASPHSQASTKTLPATGEATSMLSLLGL
                     TLIGFVGAWTKKKEH"
     gene            5630..7831
                     /locus_tag="AC1PIW_001351"
     CDS             5630..7831
                     /locus_tag="AC1PIW_001351"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIP6606283.1"
                     /translation="MKEINYTGEEVVALTATYLPEEDVIFVKKALDFATEAHKSQFRK
                     SGEPYIVHPIQVAGILAGLKLDAVTVACGFLHDVVEDTDVTLDDMEAEFGPEVRHIVG
                     GVTKLGKVEYKSHEEQLAENHRNMLIAMSQDMRVILVKLADRLHNMRTLKHLRKDKQE
                     RISRETMEIYAPLAHRLGISSIKWELEDMSFRYLNEIEFYKITRMMSEKRREREELVN
                     EVVEKLRDYTEERHLHGQIYGRPKHIYSIYRKMHDKKKRFDQLYDLIAIRCIMDTPSD
                     VYAMLGYIHELWKPMPGRFKDYIANQKANGYQSIHTTVYGPRGPIEFQIRTMEMHQVA
                     EYGVAAHWAYKRGGKATASEKELRWINNLIELQEGAGDAQTFVNSVKDDIFTERIYVF
                     TPDGAVRELPKDSVPIDFAYEIHTKVGERATGAKVNGRMVPLTTKLKTGDQVEIITSA
                     NSFGPSRDWVNLVKTHKARNKIKQFFKNQDKELSVSKGREMLQNLLQENGYVPNQYLD
                     RRHMDEVLQKTSYKTDESLFAAVGFREVSAISIFNRLTEKERREAERAKAKAVADELV
                     NGGEVKHDNKDSLKIRHEGGVVIEGASGLLIRIAKCCNPVPGDEIVGYITKGRGVAVH
                     RVDCMNLKSQENYDVRLIDVDWEDENSTKEYMANIDIYGLNRSGLLNDVLKVLTNASK
                     NISSVNAQPTKDMKFATIHVSFGISNLATLTSLVDKIKSVPEVYSVKRTNG"
     gene            7833..8276
                     /gene="dtd"
                     /locus_tag="AC1PIW_001352"
     CDS             7833..8276
                     /gene="dtd"
                     /locus_tag="AC1PIW_001352"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIP6606284.1"
                     /translation="MKIVLQRVSQASVTIEGSIHGQIEQGLLLLVGVGPDDSQEDLDY
                     AVRKIVNMRIFSDEAGKMNKSVQDVAGKILSISQFTLFADTKKGNRPAFTGAAAPALA
                     SQLYDAFNQALSAFVPVEVGVFGADMAVSLVNDGPVTIVLDTKNK"
     gene            8352..8537
                     /locus_tag="AC1PIW_001353"
     CDS             8352..8537
                     /locus_tag="AC1PIW_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606285.1"
                     /translation="MKKLLTNHFFYLTIAFLLILVIYFSGIDKRWIILASFLYFIPSQ
                     ILYRRRLRERLQEDQPK"
     gene            8692..8832
                     /locus_tag="AC1PIW_001354"
     CDS             8692..8832
                     /locus_tag="AC1PIW_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HIP6606286.1"
                     /translation="MSTFEVLTLMFVAGNFVIALVMLVLELVKTTKKITVYNFGEFTV
                     IF"
BASE COUNT         2496 a         1681 c         2122 g         2655 t
ORIGIN      
        1 cggatcagct gactaattct ttcgaccaag aaaagaagac cttagcttct gaggaggtta
       61 aggaacgaat agatttggct caagctttta atgagagttt gaacaatgtg gtttcggagg
      121 atccgtatac caaatcacga catgaagctg gacgggtaga gtatgcacgg atgctggaat
      181 tgcatgaaaa gataggttat gttgaaattc caaaaattga ggtcaagctt cctgtttatg
      241 caggaacttc tgagaccgtt cttcaaaaag gtgtagggca tttggaagga acatccttac
      301 cgattggggg gagcgatacc catactgtct taacggctca tacaggttta cccaaagctc
      361 ggttattcac agacttaacg aaggtaaaaa tcggtgatac tttctacatt cacaatatcg
      421 tagaaacatt agcgtatgaa gtagatcaaa tagtggttgc tgaaccaact caatttgagg
      481 aattgctggt caagcctggg caggactatg caaccttgct aacctgtacg ccttacatgg
      541 tgaatacgca ccgtttattg gtacgcggtc atcgtattcc ttatgttgca gaagagcatc
      601 ttaaagcgtc tgaaaatgca gagaagcgta ttcttattcg ttatcttctt tatgcgctcg
      661 gaatgtttgt cggtatttta ctgattttct tattaacaaa aaggcggaaa aataaaaaga
      721 aagtgacgag taatgatgaa acgttcacaa cttagaaagg gaatatctaa acagtccata
      781 ctcttgaagc ttttatttct ctttggttta gtagtgacgc tatatccgtg gatttctcaa
      841 tattattatc gttatgactc tgattataaa atccaatcat tttatgaaca agcacaacag
      901 ttaccgagtg aagaagttct gaaaaggttg gagcatgcta gggcctacaa ccagaccttg
      961 gaacctcaca aattgcaaga tccttacaca gaagaagaga aggctggggt agtggaatac
     1021 gctcgtatgc ttgaggtgaa ggagaaaatt ggttttgtag aaattccgaa aatcaacgag
     1081 agaattccta tatacgctgg gacaactgag gaagtattac aaaaaggagc ggggcatttg
     1141 gaaggttcct ccttgccagt tggtggagaa agtactcata ctgtgattac ggcgcatcgt
     1201 ggtcttccta atgcttcgct atttaccaat ctcgatcatt taaaagtcgg ggatcaattc
     1261 tacattcata atatagctga ggtattggct tatgaggtgg accagatttt agttgttgaa
     1321 ccgaataatt ttgatcctgt tatagttcaa aacggaaaag actatgcaac cttgctaacc
     1381 tgtactcctt atatgattaa cagccatcgt ttgttggtac gtggacatcg cgttccttat
     1441 gaaccgaaag ttgcaatcaa gcaaaaaccg gcattctttt tggatatgct aacgctatcc
     1501 tacttggtag ccctattaat catagtagtg attgttatta tcctctatct tctccgtagg
     1561 aagaagtatc aaaaggggga aatgaaatga tgaaaagaat aggatatgtt ttcatattgg
     1621 ttggtttgtt gcttccttta gtgttgctga caaatatgtc agtgcaggag tttcaagttt
     1681 tccaacagta tcagtattat cgtaggaatt ctaaattctt ttcgacagag caactcgaag
     1741 aaattaaggc ttatggggaa caggttagtg gtggtgactt acccacagtt gatccctttg
     1801 cagaaaaaaa tgattcaaat agtaccgatg tgttatcttc tgaattaatg gaaggggcaa
     1861 ttggttattt gagtattcca aaaattgaaa ttcgacaacc tatttatgtt ggggcaacca
     1921 gtgaacattt aaatgatgga gttgcttctg ttataggaac agctttacct ataggtggaa
     1981 taggtcgacg aagtgtcatt gccggtcacc gtagttggta caatgatctc agatttttcc
     2041 gactgtcaga gttgagagaa ggcgataaga tttttattga ggttggtggg aggacgctga
     2101 cgtatttggt aaaaaataca gaagtgataa aagcaacaga ttggcaaaag ttattacctg
     2161 ttgagagtca ggatatggta accttgttga catgtgatcc actagttcct ccttttgatt
     2221 atcgcttgtt agtcaacgct tatcgcgaat cagatgtaat agaggaagct gatgaagtga
     2281 gtcagaccag tcaacgtgaa ataaagcagt ttcagcaata ttcattcagt cttgtatttt
     2341 acctgacgat atttggttgg gtcttgttgc tttatatcct ttaccgtttt attagaacct
     2401 atatttggta ggatgaaggg tgtttttggc gtatgcagag gtcaaaagag ggttgaaaca
     2461 aagataggct tcctcctagt gagcatgaat ttatactttt cgaaaatcaa aagcagacat
     2521 tgttaacttg atttgatgaa cttcggttct atctgcatct gcgtcgccta gtctgatttt
     2581 tattttcatt gagtattaac gaaaattggt taagaaatta gtccaaaaca atgggagcaa
     2641 aagaaaaaca cagacagctt agctggattt catcaaaatc agcttgagtc tatctgtgtt
     2701 tttttattga gtgcaaaagg gtattggtat agtgacaagt cgtacgaacg cctctttata
     2761 ctcttcgaaa aacaaaatta tccgttgtca acttaccttg atgaactcca gttctatctt
     2821 cgacttcgtt tcctaggcta cttttgattt tcattgagta ttaagaggtt cgtgacgagt
     2881 caaaagcaat gaggcttgaa caagggctag tttttgctca gtctcgtcta ttttaatgtt
     2941 cttttttctt agtccatgcg ccgacaaaac caatcaaagt gagaccgagc agactaagca
     3001 tagaagtagc ttctcctgtt gcagggagtg tcttggtgct cgcttgtgaa tgtggactag
     3061 ccaatttatg ttgtggagca ggagcaggtg gatttaccgc tggaagagta attcgttgac
     3121 cagattcgag tacaatatct tgacctgtgt taccttgatc tgactgctgt tcatctggag
     3181 tcactacttt agattcagtg tagacaaatt tgaattcgcc aaatccttcg ctggcagttg
     3241 aagcctgtag gtagacaata cattcttggt cagtaactag agatttcgca cggtctgctg
     3301 tcaagaagcg gaggtcgagt cccttgatac tatctgtaaa ggtccagtta ttgtctgctg
     3361 taggattgat aactttctca gcgataatat aattgatgat ggcttggcgg ttttccaggt
     3421 tcaacaatcg gttgatagaa gcttcgcgga ccccagggaa agtaccgttt gcacggtagt
     3481 tgttggtcac cacgatgaat tcttgatcag ctgtcacatc ttgcccattg tattgcagat
     3541 tccgaactct gctggcagtt tcgttgacta tcttaccatc acgatcgtat ttatttggtt
     3601 gcgtaatatc gtactgataa gttactccgt caataacgtc aaaattgtaa gtgcggaaat
     3661 ctgtgttgac taggttttgt ggctctgttg agcttaggtc aacttggttg aattgccctg
     3721 cggacatttc caaccattct ttgagttggg caccgttgac ttttaagatg gctacaacgt
     3781 tgtcgtagag gtaaaggtct gctacgttct tgatggcgat tgggccagca ggaatgtctg
     3841 tataggctga agcatcgcct cgagtaccag ccttaaatgg tgcagccgca gataggattg
     3901 gtagattagc ttctgaagta cccgccaact gttgttttgc ataccaaatc tgtgcattgt
     3961 taacaatctg tacagatgga tcatcttgaa caagggcaaa gaagctgttg attggagcag
     4021 ttgtttcacc gacttgttga cgaacatatt tgatggtttc gttatgggct tctttagcaa
     4081 ggtcgatgat gcgaccatct gctacagatg attttgtatc gattttacga atagccgctt
     4141 tgctagaagt agttgtccat ttgccatctt taaagaccag attgaggtcg atgacgccga
     4201 ggtgatcacc gtatttaccc gccatggtga caggagtacc gttgatttta ccatttgtat
     4261 catctacgcc agagtattta gcatagaagc ttggtttttc ggcagttcct gggaattctg
     4321 cgtgggagtg acctgtgata accgcatcaa ctcctgacag gctggcgatt tggtaaccga
     4381 cattttcttc gccgacttca tattggtcat cgccgatacc tgagtgggaa aggaccagaa
     4441 cgatgtctgc gccattttct cgcatggttg ggataatatc gcgaacggct tcaactgcat
     4501 cacgaacaat cacttttcct tctagataag ctttgtccca gttgagaatt tgagggggaa
     4561 caatccctgt aacaccgaca tttagtgtaa cctttttacc ctcggtgtct gtgaatgtct
     4621 ttttcacaat ggtataaggg gtgtagagaa agtctttcgt agtcggatcg aggacattgg
     4681 cgttgactag aggcatgttg gctgtacgaa ttactttttc gaggtaggca aggccgtagt
     4741 taaattcgtg gttgccaagt gtgccgacgt caaatcccag agtctctaag gcggcgtaca
     4801 taggatgttg ttcaccttct tcaataggat caacgataga cttgtaattt ccaagaggag
     4861 taccttgaat ggtgtctcca ttatctacca atacgacatt tggattttct ttttttgctt
     4921 cttcgataag aacagctgtt tttgctagac ccagtgtctc tacaggttta tcttggtaat
     4981 agtcatagtt gaccaagttc gtatggaggt cagttgttgc gagaatgcga acatcaacag
     5041 tttgtccttc aacaggctga ctagtttcag ttgcagcagg agtgaccgtg cgtgcttctg
     5101 aagtagttgt gtcagttgtc tcgcttgtag gatcactagt ttcagctgtc tcggaagagc
     5161 ttggattttc agcttcggta gtggctggaa ccggagattc tgtattatca gcttcctctg
     5221 tggtgtcaga ttcaactggt acagcctgac ttgcttctgt tgaagaagct ggagttgtgt
     5281 tgagaatctc ttcggcttga gctaattttg gattgcttgc agctaagaga gctagtgtga
     5341 gtgctacggc acacttactg aaacgaaaat tcataagaat cctccatttt ctttttgtaa
     5401 taatcttcta caatcattat acagctaaga aatgcaaaat tcaagactaa ttctgaacgt
     5461 tcgtttgaaa aacgagtgaa aataacaaga aaacgaaaga aaacaagaac gttcaccgaa
     5521 tctgaacttg gtttggattg tttgtaatta gtagtcttat gttagattcc tttttctttt
     5581 ttcgtgaaaa tggtagaata gtaggaaata taagaggaag agtagagata tgaaagaaat
     5641 taactacact ggggaggaag tggttgcttt gacagccacc tatttaccgg aagaagatgt
     5701 tatatttgtc aagaaagctt tggattttgc gacagaggca cacaagtcac aatttcgtaa
     5761 atcgggcgag ccctatattg tacacccgat ccaggtagca ggtattttag caggactcaa
     5821 gctggatgct gtgacggttg cctgtggttt cttacatgat gtggttgaag atacggatgt
     5881 aactttggat gatatggagg ctgaatttgg tccagaggtt cgccatattg tcggcggtgt
     5941 gaccaaactg ggtaaggttg agtataagtc tcatgaggag cagttggctg aaaaccaccg
     6001 aaatatgctg atagccatgt cccaagacat gcgggtcatt ctggttaaac tggcagaccg
     6061 ccttcacaac atgcggactc ttaagcactt gcgcaaggac aagcaggaac gtatttcacg
     6121 tgaaacaatg gaaatctatg caccgcttgc ccaccgtcta ggtatttctt ctatcaagtg
     6181 ggaattggaa gacatgtctt tccgctatct caatgaaatt gaattctaca agattactcg
     6241 aatgatgagt gagaagcgtc gtgaacggga agagttggtc aatgaagttg ttgagaagct
     6301 aagagattat acagaagagc gccatctaca tggtcagatt tacggtagac ccaagcatat
     6361 ttactctatc tatcgcaaaa tgcacgataa gaaaaagcgg tttgatcaac tatatgactt
     6421 gattgccatt cgttgtatca tggatactcc gagcgatgtc tatgctatgt tagggtatat
     6481 tcatgaattg tggaaaccta tgccaggtcg cttcaaggac tacattgcca accaaaaagc
     6541 taacggctac cagtctattc ataccacggt ttacggaccg agaggtccaa tcgagttcca
     6601 aattcggacc atggaaatgc accaggttgc agagtacggg gttgcggctc actgggccta
     6661 caagcgtggt ggcaaggcaa ctgctagtga aaaagagttg cgctggatta acaacctgat
     6721 tgaactccaa gaaggggctg gggatgcgca aacctttgta aattctgtca aggacgatat
     6781 ttttacagag cgtatttacg ttttcacgcc agatggtgca gtgcgtgagt tgcctaagga
     6841 ctccgtaccg attgacttcg cctatgagat tcataccaag gtcggcgagc gggcgacagg
     6901 tgccaaggtc aacggccgta tggtgcctct gacgaccaag ctcaagacag gcgatcaggt
     6961 ggaaatcatc acctctgcca actctttcgg tccaagtcgt gactgggtca acttggttaa
     7021 aactcacaag gctcgcaata aaatcaagca gttcttcaag aatcaggaca aggaattgtc
     7081 tgtttcaaaa gggcgtgaga tgcttcagaa cctactccaa gaaaatggct acgttcccaa
     7141 ccaatacctg gaccgtcgcc acatggatga ggtcctgcaa aagaccagct ataagaccga
     7201 cgaatccctt tttgcggcag ttggttttag agaagtttct gccatatcaa tctttaaccg
     7261 tctgacagag aaagaacgtc gcgaagctga gcgggctaag gccaaagctg tcgcggatga
     7321 attggtcaac ggtggcgaag tcaaacacga caacaaagat agtctgaaaa tccgtcacga
     7381 aggtggcgtg gtcattgaag gagcgtcagg cttgctgata cggattgcca aatgttgtaa
     7441 cccagtacct ggcgatgaaa ttgtcggcta catcaccaag gggcgtgggg tagcggtcca
     7501 ccgtgtggac tgtatgaacc tcaagagtca agaaaactac gatgttcgct tgattgatgt
     7561 ggactgggag gatgagaatt cgaccaagga atacatggca aacattgaca tttacggtct
     7621 caaccgctca ggtcttctca acgatgtcct caaggttttg accaatgcca gcaagaatat
     7681 ttcgtcagtc aatgcccagc caaccaagga tatgaagttt gcgaccatcc acgtttcctt
     7741 tggcatttct aacctggcaa ccttgaccag tctggtggac aagatcaagt cggtgccaga
     7801 agtttactca gtgaaaagga ccaacggata agatgaaaat tgtattacaa cgtgtatcac
     7861 aggctagtgt gaccatcgaa ggaagtatcc atgggcaaat tgaacaaggg cttttgctcc
     7921 tagtcggtgt ggggccagat gacagccagg aagacctgga ctatgctgtt cgtaaaattg
     7981 tcaatatgcg gattttttca gatgaggcag gcaagatgaa caagtctgtt caagatgtgg
     8041 cgggcaagat tttatccatt tcccaattta cgctttttgc ggatacaaaa aaaggcaatc
     8101 gccctgcctt tacaggagcg gcagctcctg ccctagccag ccaactctat gatgctttta
     8161 accaggcctt gtcagccttt gtgcctgttg aagtgggcgt ttttggggcg gatatggcgg
     8221 ttagcctggt caatgatggg ccggtgacca ttgttttgga tacgaaaaat aagtaaaact
     8281 tttggcaatg gtatgtactc tatttgtgta ttttgatata ctaatattga aaaataggta
     8341 tggaggatgc tatgaaaaaa ttgctcacaa atcatttctt ttatttgacg attgcctttc
     8401 ttttgatttt ggtgatttac tttagtggaa tagataaacg ttggattatt ttggcaagtt
     8461 ttctttactt cattccatcg cagattttat accgtcgtcg cctaagagag cgactccaag
     8521 aagaccagcc caagtaggct ggttttttga tgtgtaaatt ggactacaat tctttattaa
     8581 ctgtgctata atagtttttg cagaaaagta aagacggtgg ctctaatttc tgaaaggtag
     8641 gtggtgtcta tgggcaaatc atcaaaatct aacagaaagg agaagctatc tttgtcaacc
     8701 tttgaggtct taacactcat gtttgtagct ggtaactttg ttatcgctct cgtgatgtta
     8761 gtacttgaat tggtgaaaac gacaaaaaaa ataaccgtct ataactttgg cgagttcacg
     8821 gttatttttt aataatttat aacaagccac cgtcttaaac ggttctgcaa ggggtagttg
     8881 tttccagcaa ctaccttttc ctatgtttat tatacattgg caggtctacc aagtcaagtt
     8941 ggacacttat cttt
//
LOCUS       DBNFCK010000074         8882 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.074, whole genome shotgun sequence.
ACCESSION   DBNFCK010000074 DBNFCK010000000
VERSION     DBNFCK010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8882
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.074"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(177..1658)
                     /gene="guaB"
                     /locus_tag="AC1PIW_001355"
     CDS             complement(177..1658)
                     /gene="guaB"
                     /locus_tag="AC1PIW_001355"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIP6606287.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPHDIDLKTQLAPNLTL
                     NLPIISAAMDTVTDSKMAIAMARAGGLGVIHKNMSIAEQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEAEKLMATYRISGVPIVETLENRKLVGIITNRDMRFISDYSQPISTNMT
                     SDELVTAPVGTDLATAEAILHKHRIEKLPLVDENGRLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIKEIRDHFPTR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAGVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGHAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKQGSKDRYFQASVNEANKLVPEGIEGRVAYKGSVADMIFQMVGGLRSGMGY
                     VGAGNLTELHENAQFIEMSGAGLKESHPHDVQITNEAPNYSVQ"
     gene            complement(1890..2915)
                     /gene="trpS"
                     /locus_tag="AC1PIW_001356"
     CDS             complement(1890..2915)
                     /gene="trpS"
                     /locus_tag="AC1PIW_001356"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIP6606288.1"
                     /translation="MSKPIILTGDRPTGKLHIGHYVGSLKNRVLLQDAGQHELFVFLA
                     DQQALTDHAKEPQKIVESVGNVALDYLAAGLDPAKTTIFIQSQIPELAELTMYYMNLV
                     SVARLERNPTVKTEIAQKGFGEGIPAGFLVYPVSQAADITAFKANFVPVGNDQKPMIE
                     QTREIVRSFNHAYQTDILVEPEGIYPENEAAGRLPGLDGNAKMSKSLGNGIYLADDMD
                     TLKKKVMSMYTDPNHIRVEDPGKIEGNMVFHYLDVFGREEDKAEIEAMKEHYQRGGLG
                     DVKTKRYLLDILERELGPIRERRLEFAQDMGQVYAMLEAGSQRARQVAAKTLDQVKSA
                     MGINYFK"
     misc_binding    complement(2962..3168)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3270..4142
                     /locus_tag="AC1PIW_001357"
     CDS             3270..4142
                     /locus_tag="AC1PIW_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIP6606289.1"
                     /translation="MKERIKDFISVTLGSVVMAIGFNSFFLENNIVSGGVGGLAIALN
                     ALLRWSPSDFVLYCNIPLLIICWFFLGKSVFIKTVYGAIIYPLCIKLTAGLPNLTENP
                     LLAAIFGGIILGFGLGLVFLGNSSTGGTGILIQFIHKYTPLSLGLTMAIIDGIIVGLG
                     FVAFDTDTVMYSIIALMTITYIVNRMMSGTQSSRNVMIISQKSEEIKDYITKVADRGV
                     TELPIIGGFTGVDKRMLMTTISIPEMQKLETAVLEIDETAFMVVMPASQVRGRGFSLQ
                     KDHKHYDEDILIPM"
     gene            4207..5829
                     /locus_tag="AC1PIW_001358"
     CDS             4207..5829
                     /locus_tag="AC1PIW_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIP6606290.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTAGNTYGLIGANGAGKSTFL
                     KILAGDIEPSTGHISLGPDERLSVLRQNHFDYEDERVIDVVIMGNEQLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNISEDLHYQNMSELTNGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSINWLEDFLIDFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKIFVGNYDFWKQSSELAAKLQADRNAKAEEKIKELQEFVARFSANA
                     SKSKQATSRKKMLDKIELEEIIPSSRKYPFINFKSEREIGNDLLTVENLKVVIDGETI
                     LDNISFILRPGDKTALIGQNDIQTTALIRALMGDIEYEGTVKWGVTTSQSYLPKDNTR
                     DFDTNESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKAFKESIIFASHDHEFIQTLANHIIVI
                     SKNGVIDRIDETYDEFLENAEVQAKVQELWKA"
     gene            6136..8715
                     /locus_tag="AC1PIW_001359"
     CDS             6136..8715
                     /locus_tag="AC1PIW_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HIP6606291.1"
                     /translation="MKKIFTKTSIYYLLSFLIPLTIISIVLAFQGIWWGSDTTILASD
                     GFHQYVIFNQTLRNTLHGDGSLFYTFSSGLGLNFYALSSYYLGSFLSPIVFFFDLQSM
                     PDAIYLVTIVKFGLTGLSTYFSLKGIHKNLKEEWALLLATSFSLMSFSTSQLEINNWL
                     DVFILLPLVLLGLHRLLKKQGPILYYITLTCLFIQNYYFGYMVAIFLTLWTLVQLSWI
                     DSQRIKRFINFTIVSILSALSSMFMLLPTYLDLKTHGETFTKIVNLKTEDSWYLDFFA
                     KNLVGSFDTTKFGSIPMISVGLVPLILALLFFTLKEIKPTVKLSYALFFTFIISSFYL
                     QPLNLFWQGMHAPNMFLYRYAWALSITVIYLAAETLVRLRQVSIKNFTLIVSFLLICF
                     TSTFIFRDHYEFLTDVNFLLTLEFLIAYFILFVAMIRYKSSLKWINIVLLFFTFLELG
                     LHSHYQVQGISDEWHFPSRSNYEEKLTDIDSIVKSTKTTTDSFYRIERLLPQTGNDSM
                     KFNYNGISQFSSIRNRASSSVLDKLGFRSDGTNLNLRYQNNTIIADSLFGVKYNLATT
                     DPNKFGFTLNQSQSTINLYENSFNLGLALLTEGIYKDVNFTNLTLDNQTNFLNQLTGL
                     SQKYYHTLSDVVSQNTVELSNRMTVNKVDNEDAAKATFLVNIPANSQVYLNLPNLTFS
                     NENQKKVVITVNNQSSEFTLDNAFSFFNVGSFTTDVQVQVNVYFPENNQVSFDKPQFY
                     RLDLLAFQQAISILQEKQVVTKTDGNKVTVDFVTDKESSLLLTLPYDKGWNATIDGKP
                     IKIQKAQDGFMKVDVSPGQTKLVLTFVPNGFYLGLLISFGAVFVFFSYQFIGYYYSKN
                     REY"
     gene            complement(8784..8856)
                     /locus_tag="AC1PIW_001360"
     tRNA            complement(8784..8856)
                     /locus_tag="AC1PIW_001360"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8821..8823),aa:Asn,seq:gtt)
BASE COUNT         2643 a         1850 c         1577 g         2812 t
ORIGIN      
        1 aattgaagaa gtttgcgaaa gatagcaagt tattaaactc ttagaaatat tgatatttcg
       61 gcgtttctga gagttttttt attttcaata taaagaaaaa aagattgaag aaaattgtcc
      121 aaaatggact ttttcttcaa tctgaaagac cctgccaaga gggtcttgtt tttgttttac
      181 tgaactgaat agtttggagc ttcgtttgtg atttgaacat cgtgtggatg gctttctttc
      241 aaaccagcac ctgacatttc gatgaattgt gcattttcat gcaattcagt caagttacca
      301 gcacctacat agcccatacc tgagcgaagt ccaccaacca tttggaaaat catatctgca
      361 acagaacctt tataggctac acgtccttca attccttctg gaacaagttt gttggcttca
      421 ttgacagatg cttggaagta acggtctttt gaaccttgtt tcatggcagc gatagagccc
      481 ataccacgat aggtcttgaa cttacgacct tggaagattt ctgtctcacc tggtgcttca
      541 tcagttcctg caaacataga accaagcata acggcatgac caccagcggc aagtgccttg
      601 acaatatctc ctgaatactt gataccacca tcagcgataa tcgttttacc atattcacgc
      661 gcaacaccag ctgcatcata gatagctgtc acttgtggta caccgacacc tgcgataaca
      721 cgtgttgtac agattgaacc tggtccaata ccgaccttca caacatcaac accagcttca
      781 taaagagcac gagctccttc agctgtggca atgttgccgg caatcaaggt acgagttggg
      841 aagtggtcac ggatttcttt aatcttgcga agaacgcctg ctgagtgacc gtgagctgta
      901 tcgatgacaa tcgcgtctgc acccgcttca aaaagggctt ctgcacgttc aaaagtatca
      961 gatgtaacac caacagcacc cgcaaccaag agacgaccaa attcatcctt cgcagcattt
     1021 gggaactcaa tcactttctc aatatctttg atggtaatca agcctgacaa acgaccattt
     1081 tcatcaacca atggcaattt ttcaatacga tgcttgtgaa gaatagcctc cgcagtcgca
     1141 agatctgttc caactggcgc tgtcaccaat tcatcgcttg tcatgttggt cgaaattggc
     1201 tgtgagtaat ctgaaatgaa acgcatatcg cggttggtga taataccgac caacttacga
     1261 ttttccaatg tttcaacgat tggcacaccg gaaatacgat aagtagccat caatttctca
     1321 gcttcagcaa tggtgtgttc tggtgttaag aagaatggat cgataatgac accattttca
     1381 gaacgtttta ccttacgcac ctcatctgcc tgctccgcga tagacatgtt cttatggatg
     1441 acgcccaaac cacctgcacg cgccatagcg atagccattt tgctatctgt tactgtatcc
     1501 atggcagcag aaataatcgg aagattaagg gtcaaatttg gtgctaattg tgtttttaaa
     1561 tcaatatcat gtggtaatac atgactttca gccggaataa gtaatacatc gtcgaaagta
     1621 aagccttttt tcaaaaattt agtgtcccag tttgacattt tcttcctctt ttcttattta
     1681 atatgtagca tgattgctat ttgtatttta attatgataa cattttgaaa tcatttgtca
     1741 atcattattc gctttaatat catcattcct aaaaattcag aaaattctat tcagtcttat
     1801 acataaaatt cgaacgtttg ttttttgcta cgtcgtaaaa caaatgaaaa aaacaacatt
     1861 ttctcaacat tgctctttca ttgttaacct tatttaaaat agttgattcc catagcagat
     1921 ttcacttggt caagagtttt cgcagcaact tggcgagctc tttggctacc tgcttccaac
     1981 attgcataga cctgtcccat atcctgtgca aactccagac ggcgttcacg gattggtcct
     2041 aattctctct ctaaaatgtc cagcaaataa cgtttggtct tcacatcgcc caaaccacca
     2101 cgttgatagt gctccttcat agcctcaatc tcagccttat cttcttcgcg cccaaagacg
     2161 tcaagatagt ggaaaaccat gtttccttca atcttacctg ggtcttcaac acggatgtgg
     2221 ttgggatcag tgtacatgga catgactttc ttcttgagcg tatccatgtc atctgccaga
     2281 taaataccat ttccaagtga tttagacatc ttggcattgc cgtccaaacc tggcaagcga
     2341 ccagctgcct cattttcagg ataaatgccc tccggctcta ccaaaatatc agtctggtaa
     2401 gcatggttaa agctacgaac gatttcacga gtctgctcaa tcataggttt ctggtcattt
     2461 cctactggaa caaagtttgc cttgaaggct gtaatatccg ccgcttgtga cacaggatag
     2521 accaaaaaac cagctggaat tccttcgcca aatcccttct gagcaatctc agtcttcact
     2581 gtaggattgc gttctagacg tgcaacagat accaagttca tgtagtacat ggtcaactct
     2641 gccaattcag gaatttgact ctggatgaaa atcgttgtct tagctggatc aagtcctgcc
     2701 gctagataat ctagtgctac gtttccaacg gattcaacaa tcttttgcgg ttcctttgca
     2761 tgatctgtca acgcttgttg gtctgctaaa aagacaaaaa gttcatgttg acctgcatcc
     2821 tgtaataaaa cacgattttt caacgaacca acataatgtc caatatgtaa tttgcctgtt
     2881 ggacggtctc ctgttaggat aatcggttta ctcataaaat gttctccttt tacataataa
     2941 aagcccacgc acagaaaact gtgcgagggc gtcaaggaca cggtgccacc tcacttataa
     3001 gcattaaata agcttacatc tttttgcaca taaggcctgc tagccgaatc cttatttatt
     3061 cagattctat tcactcagtc cattcataag cgactcttgt ttgtttacac caaccacaaa
     3121 ctctctaaaa attatccact tactactctt ctgattgatt cctattataa ccgcaaaaaa
     3181 ttagaaagtc aagctgacaa agccttgacg atactgtcta aattagggat aatagaaatt
     3241 gcttttataa cactaaccaa ggagaatata tgaaagaacg tataaaggac tttatctctg
     3301 tcacactcgg atcagttgtc atggccattg gatttaacag tttttttcta gagaataata
     3361 tcgtatctgg tggtgtcgga ggtttagcaa tcgcactcaa tgctcttctg aggtggagtc
     3421 cgtctgattt tgtcctctac tgtaacatcc ctttattaat catctgttgg tttttcttag
     3481 gaaaatctgt atttatcaaa actgtctatg gtgctatcat ctatcctctt tgcatcaagt
     3541 tgacagcagg tttacccaac ttgacagaaa atcctctcct agctgctatt ttcggtggaa
     3601 ttatcctagg ttttggactt gggcttgtct tccttggaaa ttcttctact ggtggaacag
     3661 gtattcttat tcaatttatt cacaagtaca cccctttatc attaggtctg acaatggcta
     3721 ttattgatgg tattatcgtt ggtcttggat ttgttgcctt cgatactgat actgttatgt
     3781 actcgattat tgccctaatg accattactt atattgtcaa ccgcatgatg tctggtaccc
     3841 aatcttctcg aaatgtcatg attatttctc aaaaatcaga agaaattaaa gactatatta
     3901 ctaaagttgc tgatcgcggt gtgacagaac ttccaattat cggtggcttt acaggagttg
     3961 acaagcgcat gttgatgact accatctcta ttccagaaat gcagaaactt gagacagcgg
     4021 tattagaaat tgatgaaact gcctttatgg ttgttatgcc tgcgagtcag gtgcgtggac
     4081 gtggcttcag tcttcaaaaa gaccataaac attatgatga agatattttg attccaatgt
     4141 aattcattga aaattcaagt tctttctagt acaataaaac taatagacag aaatagagga
     4201 gaaactatgc ttacagtatc agacgtgtcg cttcgcttta gcgatcgcaa attatttgac
     4261 gatgtcaata tcaaatttac agcaggaaat acctacggtt tgattggtgc aaacggggcc
     4321 ggtaaatcta catttttgaa aattcttgct ggtgacatcg agccatcaac aggtcatatt
     4381 tcacttggac cagacgaacg cctttctgta ctacgtcaga accatttcga ctatgaagac
     4441 gaacgcgtca ttgacgtagt tatcatgggg aatgagcaac tttacagcat catgaaagaa
     4501 aaagatgcta tttacatgaa ggaagatttt tctgatgaag atggtgttcg tgctgctgaa
     4561 ttagaaggtg agtttgctga acttggtggc tgggaagctg aaagtgaagc ttctcaattg
     4621 ctccaaaacc tcaacatttc agaagacctc cactaccaaa acatgagcga attgaccaac
     4681 ggtgagaagg ttaaggtcct cttggctaag gcactttttg gtaaaccaga tgttcttctt
     4741 ttggacgagc caaccaacgg tctggacatc caatctatca actggctcga agatttcttg
     4801 attgactttg aaaatacggt tatcgtcgtg tcacacgacc gccacttctt aaacaaagta
     4861 tgtacccata tggccgacct tgacttcggt aaaatcaaga ttttcgttgg taactatgac
     4921 ttctggaaac aatctagcga actagcagct aaactacaag ctgaccgtaa tgcaaaagca
     4981 gaagaaaaaa tcaaagaact acaagaattc gttgcccgtt tctctgctaa tgcttctaag
     5041 tctaagcaag ctacttcacg taagaaaatg ttggacaaaa tcgagttgga agaaatcatt
     5101 ccttctagcc gtaagtatcc atttatcaac ttcaaatcag aacgtgaaat cggtaatgac
     5161 ctcttaacag ttgaaaactt gaaagttgtt attgatggtg aaacgattct tgacaatatc
     5221 agctttatcc tgcgcccagg tgacaagact gctcttattg gtcaaaacga catccaaaca
     5281 actgctctca ttcgtgctct tatgggcgat attgaatatg aaggtactgt caagtggggt
     5341 gtcactacta gtcaatccta cttaccaaaa gacaatactc gtgactttga tacaaacgaa
     5401 tctatccttg attggctccg tcaatttgcc agcaaggaag aagatgacaa taccttcttg
     5461 cgcggtttct tgggacgtat gctcttctcg ggtgatgagg ttaacaaacc tgtaaacgtc
     5521 ttgtcagggg gcgaaaaagt gcgcgtgatg ttgtcaaaac tcatgctcct caagtcaaat
     5581 gtcttggtct tagatgatcc aaccaaccac ttggacttgg aatcaatttc cagtctgaac
     5641 gatggtttga aagcttttaa agagtccatc atctttgcca gccatgacca cgaatttatt
     5701 caaactttag caaaccatat catcgtcatt tctaaaaacg gtgttatcga ccgaatcgac
     5761 gaaacttatg atgaattctt ggaaaatgct gaagtacaag caaaagtaca agaactttgg
     5821 aaagcataat aaaaagaggc tgggactttt gcccagcctc tcttctcaga gttcgtgtca
     5881 acatctcagc gcagtggttg attgccagat ttgttcgtgt ttcacactcc aaatctggcc
     5941 attacgactg ttgcgaacaa agttcgcctt atctccaacc tcaaactgtc tcccagacag
     6001 tttgagctgt gcgggggtgg gagtgaaaca gtctgggaat agactgtttc agctcaacaa
     6061 ctagaaataa agacttgttg acgaactctt ttttgaccgg tcaagttctt tcccacttcc
     6121 ttttctttta aatctatgaa aaaaatattt acaaaaacat ccatttatta tctcttatct
     6181 ttccttattc cgctgactat tatttctatc gtcttagcat ttcaaggaat ctggtgggga
     6241 agtgatacaa caatattggc cagtgatggt ttccatcagt atgttatttt taatcaaaca
     6301 ttacgaaata ctttacacgg agacggctct ttattttaca cattttcaag tggtttagga
     6361 ctcaattttt atgctctatc ctcctattac ctaggttcat tcctatctcc aattgtcttt
     6421 ttctttgatt tacaatccat gcctgacgct atctaccttg tcactattgt caaatttgga
     6481 ttgactgggt tgtcaactta tttcagtcta aagggtatcc ataaaaattt gaaagaagaa
     6541 tgggcactat tactcgctac tagtttttct ctgatgagtt ttagtacaag tcaattagaa
     6601 ataaacaact ggctagacgt ttttatactc ctcccactcg ttcttcttgg attgcatcga
     6661 ttattaaaaa aacagggtcc gattctctac tacataacat taacatgctt gtttatccaa
     6721 aactattact tcggttatat ggtggctatt tttctaacat tatggacatt ggttcaatta
     6781 tcatggatag atagtcagag aatcaaaaga tttatcaact ttacaatagt gtcaatttta
     6841 tctgctctaa gcagcatgtt catgttgtta ccaacctact tggatttaaa aactcatggt
     6901 gagacattta caaaaattgt caacctaaaa accgaagact cttggtatct ggacttcttt
     6961 gcaaaaaact tagttggtag ctttgataca acaaaattcg gttctattcc catgatctct
     7021 gttggtctcg ttccattaat actcgcttta ttattcttta cattaaaaga aataaaacca
     7081 actgtcaaac tctcctatgc tctattcttt acattcatta tttcaagttt ctacctacaa
     7141 ccactcaatc ttttttggca aggtatgcat gcgccaaaca tgttcctcta tcgttatgca
     7201 tgggctctat caataaccgt tatctattta gcagcagaga cattggtacg actacgtcaa
     7261 gtaagtatta aaaattttac tctgattgtt tcatttttac tcatttgctt tacttctacc
     7321 tttattttta gagatcatta tgagtttcta acggatgtaa atttcctact cacactagaa
     7381 tttctaatag cctacttcat tctttttgta gcaatgattc gctataaatc atccttaaaa
     7441 tggattaata ttgttttatt gttcttcact ttcttagaac ttggattaca tagtcattat
     7501 caggttcagg ggatttctga tgaatggcat tttcctagcc gatcaaacta tgaagaaaaa
     7561 ttgacagata ttgacagtat tgtaaagtct acaaagacta caactgattc attttatcgt
     7621 atagaacgtc tattaccaca aactggcaat gatagcatga aattcaatta caacggtatt
     7681 tctcaattct cctccattcg aaatcgtgct tccagctcag tactagataa gctcggtttc
     7741 cgttcagatg gtaccaattt gaatcttcgc tatcagaata atacaatcat tgctgatagc
     7801 ctattcggag tcaaatataa tttagctact acggacccaa ataagtttgg tttcacactg
     7861 aatcagagtc agtctactat taatctttac gaaaatagtt ttaatctagg gttagcccta
     7921 ttgactgaag ggatttacaa agatgtcaat tttacaaatc tgactctcga taatcaaaca
     7981 aattttctta atcaactaac agggctatct caaaaatact accacacttt atcagatgtt
     8041 gtttcacaaa atacagttga actgagcaat cgaatgactg tcaacaaagt ggacaatgag
     8101 gatgctgcaa aagcaacttt cttagtaaat atacctgcaa atagccaagt atacttaaat
     8161 ttgccaaatt taacattctc aaatgagaat caaaaaaaag ttgtcattac tgtcaacaat
     8221 cagtcaagtg agtttacact agataatgct ttctctttct tcaatgtggg gagctttact
     8281 acagatgtac aggttcaagt caatgtatat tttcctgaaa ataatcaagt ttcttttgat
     8341 aaaccacaat tttatcgatt ggacttatta gctttccaac aggctatttc cattctccaa
     8401 gaaaaacaag tagtaacaaa aactgatggt aataaagtaa ctgttgattt tgtaaccgat
     8461 aaagaatcct cactcctcct tactttaccc tatgataaag gatggaatgc aaccattgat
     8521 ggtaaaccta tcaaaatcca aaaagcgcaa gatggtttta tgaaagtgga tgtaagtcca
     8581 ggtcaaacta aactagtttt aacctttgta ccaaatggtt tctatctagg tttactgatt
     8641 tcttttggtg cagtttttgt atttttctcc tatcaattca ttggatacta ttattctaag
     8701 aaccgagaat actaaacttt ctcggttttt tcttgtaaaa aacaaaaaac cttgatttct
     8761 caaggtttcc tatgctttat ctactccgcc aacagggctc gaacctgtga catcatgatt
     8821 aacagtcatg cgctctacca actgagctat ggcggaagaa atagtccgta cgggattcga
     8881 ac
//
LOCUS       DBNFCK010000075         8746 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.075, whole genome shotgun sequence.
ACCESSION   DBNFCK010000075 DBNFCK010000000
VERSION     DBNFCK010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8746
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.075"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..1816)
                     /locus_tag="AC1PIW_001361"
     CDS             complement(<1..1816)
                     /locus_tag="AC1PIW_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606292.1"
                     /translation="MESLHRKISNWGQKIFLQFKNRTPNVEGISFNRLTSNKNIDLER
                     ENPSFKQALDFAIQDDELKNVAITGNYGSGKSSLLDSYEAKYSNKKFLHISLADYKEN
                     DLSGYKENDSSESGKKLNVRQINVIEGKIINQLLHQIHPKHVKKSIFKTLSRTPRRIP
                     LLLTVYIVLFFITLSYFLKPNFLKALVTADKVFGWDANTVFSWLFLILLFIGSLTTIW
                     YIVENKTIKSLTIGGKNVSSEIDIFSDQSMRVSYFDKYLDDVLYLLNESRADVIVFED
                     IDRFENNTIFAKIKELNVLVNNKRKIAKKSSKLVFLYLIRDDLFISKERTKFFDFIIP
                     VLPVITSSNSSDKLTTTLKEMGIKSGLADDFLFRISLYIDDMRLLNNICNEFYSYQLE
                     LTHDKTGEKNALDLDLKKIFAMIVYKNIFPKDFSELQNNQGFLYSLFNEKEVRRSEKL
                     TKIDSEKLHLENKLRKIQSEHIQDEIELYGTIFKIPNGRKVVSVNDKFQDEFTSYHDF
                     ISEMLVEGSRIISYVDFDDAYRNVGIKTENMDSIFPEKDSPEFKERLDTVRSRNNSKE
                     LQQKIASLNQKRSFVEKQLIADIYTNQEIHEFAKSREEF"
     gene            complement(1911..2654)
                     /locus_tag="AC1PIW_001362"
     CDS             complement(1911..2654)
                     /locus_tag="AC1PIW_001362"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HIP6606293.1"
                     /translation="MATYETFAAVYDEIMDDSLYDKWTDFSLRNLPQDTKTILELACG
                     TGIQAVRFAQKGYEVTGLDLSYEMLELAQKKAEAAGVMMELAQADMMALEGVGDFDAV
                     TCYSDSLCYMSDREAVLRVFEGVHSVLNQGGTFLFDVHSIYQMEEVFAGYSYHENYED
                     FAFVWDTYEGEHAHSIVHELTFFVKDEASQEERFIRRDEVHEERTYPIETYVELLKEA
                     GFASVEVFADFEDEAPTETSQRWFFKCKK"
     gene            complement(2956..3315)
                     /gene="rsfS"
                     /locus_tag="AC1PIW_001363"
     CDS             complement(2956..3315)
                     /gene="rsfS"
                     /locus_tag="AC1PIW_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIP6606294.1"
                     /translation="MKELDLVKVVVQAADDKRAEDLVVIDVQGVTSLTDYFVIASSMN
                     SRQLEAIAENIREKVADVGIKSSRVEGDSNGGWVLLDLGGVVVHIFSEEMRDLYNLEK
                     LWHEGSFLEVADFLEEE"
     gene            complement(3317..3827)
                     /locus_tag="AC1PIW_001364"
                     /pseudo
     CDS             complement(3317..3827)
                     /locus_tag="AC1PIW_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935141.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
     gene            complement(3824..4126)
                     /locus_tag="AC1PIW_001365"
     CDS             complement(3824..4126)
                     /locus_tag="AC1PIW_001365"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6606295.1"
                     /translation="MWADEQVVGFALIDIDEDIQQYMLWRFMIGQEFQGRGYGQAALE
                     VVIAMAQSHPVCDHLIAAYVKGNQKMAHLLEKNNFVLDSEEERELVFRLETPGVQL"
     gene            complement(4657..5244)
                     /gene="yqeK"
                     /locus_tag="AC1PIW_001366"
     CDS             complement(4657..5244)
                     /gene="yqeK"
                     /locus_tag="AC1PIW_001366"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HIP6606296.1"
                     /translation="MIAADLTFDRQALLEKIRVAMKPARFQHVLGVEQAALVLADQYG
                     CDPKKASLAALLHDYAKEVEDQVFLDLIAKYDLDKDLLNWDNNIWHGVVGAYKIAEDF
                     GLEDEEIFQAIQRHTVGAGQMTLLDKVLYVADYIEPNRDFPGVDEARRIAKESLDKAV
                     AYETAQTISYLAKKGIPIYPQILETYNGYVVWLKE"
     gene            complement(5241..5873)
                     /locus_tag="AC1PIW_001367"
     CDS             complement(5241..5873)
                     /locus_tag="AC1PIW_001367"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HIP6606297.1"
                     /translation="MAIELLTPFTKVELEVEKKETNRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLKLDQVLLMPEFIPPHVDKKETIDEYHRYSMLKMAIAGIEGLGIETIELERR
                     GVSYTYDTMKLLKEKNPDTDYYFIIGADMVDYLPKWHRIDELVQLVQFVGVQRPRYKA
                     GTSYPVIWVDVPLMDISSSMVRSFIKQGRVPNFMVPHEVLDYIEEKGLYQ"
     gene            complement(5886..6197)
                     /gene="yhbY"
                     /locus_tag="AC1PIW_001368"
     CDS             complement(5886..6197)
                     /gene="yhbY"
                     /locus_tag="AC1PIW_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HIP6606298.1"
                     /translation="MMLTSKQRAFLNSQAHSLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKQVKE
                     I"
     gene            complement(6467..7573)
                     /gene="yqeH"
                     /locus_tag="AC1PIW_001369"
     CDS             complement(6467..7573)
                     /gene="yqeH"
                     /locus_tag="AC1PIW_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HIP6606299.1"
                     /translation="MEELFCIGCGAQIQTLDKAVAGFTPQSALEKGLETGQLYCQRCF
                     RLRHYNEISDVNISDDDFLKLLHSVGESDALVVNVIDIFDFNGSVIPGLPRFISGNDV
                     LLVGNKQDILPKSVKTGKVTQWLTERAHEIGMRPVDVVLTSAQNKQAIKDLIEKIEQH
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDKDVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYISPRKEIKPKTYQLNPEQTLFLAGLGRFDFVAGERQ
                     GFTAFFDNELQLHRTKLQGASEFYQKHAGSLLVPPTSKELKEFPELVRHEFTINEKTD
                     VVFSGLGWIRVNEKAKIAAWAPKGVDVVIRKAII"
     gene            complement(7574..8101)
                     /locus_tag="AC1PIW_001370"
     CDS             complement(7574..8101)
                     /locus_tag="AC1PIW_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HIP6606300.1"
                     /translation="MSLENYMPDFALEKAYDVTVESLKKHGIKVVFVDLDNTLIAWNN
                     PDGTPEMRQWLHDLQDAGIPVVVVSNNKYERVKRAVEKFGIEFEAFALKPFTFGINRA
                     LKRFDVQPYEVIMIGDQLMTDIRAAKRAGLKSVLVKPLIKTDSINTQINRWRERRTMK
                     KIIAKYGAIDYKREM"
     gene            complement(8285..8659)
                     /locus_tag="AC1PIW_001371"
     CDS             complement(8285..8659)
                     /locus_tag="AC1PIW_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606301.1"
                     /translation="MNPLRKELRTVAVEVSDLALDYAVRLAQSLNSTLRYHNYDSLIA
                     IAKTKGVEPKGKDCQSFSEYRQRYSLYDAKKLIYRALAWRLFDDSHADYGHALTILGL
                     DEDESGVEQIGFAFSKFTLDID"
BASE COUNT         2508 a         2023 c         1583 g         2632 t
ORIGIN      
        1 taaattcttc tctactctta gcaaattcat gtatttcttg attagtatag atgtccgcaa
       61 tcaattgttt ttcaacaaag ctccgttttt gatttaatga agcaattttt tgttgcaact
      121 ctttactatt attgcgactt cttacagtat caaggcgttc cttaaactct ggagaatctt
      181 tctctggaaa aattgaatcc atattttctg ttttaatacc tacattacgg taagcatcat
      241 caaaatccac ataggagatg atacgacttc cttccaccag catttcagaa ataaaatcat
      301 gataagaagt aaactcatct tgaaatttgt cattgacact gacaaccttt ctcccatttg
      361 gaattttaaa aattgttcca tagagttcaa tttcatcttg aatgtgctca ctctgaatct
      421 ttctcagttt attctctaaa tgcagttttt cactatcaat cttcgtaagt ttttcacttc
      481 ttctaacttc tttctcatta aacaatgaat atagaaaacc ttgattattc tgcaactcag
      541 aaaagtcttt cgggaaaata tttttgtaga caatcattgc aaatattttt ttcaaatcaa
      601 gatccaatgc atttttctca cccgtcttgt catgtgtcaa ttcaagctga taagaataaa
      661 attcattaca gatattatta agcaatctca tatcatctat ataaagcgag attctgaaga
      721 ggaagtcatc cgctaatcct gattttattc ccatttcttt taaagtagtt gtcaacttat
      781 cgctagagtt tgaactcgta ataactggaa ggactgggat gataaaatca aaaaatttag
      841 ttctttcttt tgaaatgaat aaatcatctc gtatcaggta aagaaacact agctttgatg
      901 atttcttagc tatttttcgc ttattgttga ctaatacgtt tagttcctta atcttggcaa
      961 aaatagtgtt attctcaaaa cggtcgatat cctcaaaaac aatgacatct gctcttgatt
     1021 cattcaaaag atacaacaca tcatctaggt atttgtcaaa ataggaaact ctcatcgact
     1081 gatcagaaaa aatatcaatc tctgatgaaa catttttgcc accaattgtc aatgatttta
     1141 tcgtcttatt ttcaactata taccaaatcg ttgtaaggga gccaataaaa agaagaatca
     1201 aaaataacca gctaaatacg gtattggcat cccacccaaa taccttgtca gcagtgacta
     1261 aagcttttaa aaaattgggt ttcaaaaaat aagaaagtgt aatgaagaat agtacaatat
     1321 atacagttaa taaaagcggt attcttcttg gagttcgaga caatgtttta aaaatcgatt
     1381 tctttacatg ttttggatga atctgatgaa gtaattgatt tatgatcttg ccttcaatca
     1441 cattaatctg tcgtacattt agtttctttc ctgactctga tgaatcattt tctttatagc
     1501 ctgataaatc attttcttta tagtcagcta gagaaatatg gaggaatttt ttattagagt
     1561 acttcgcttc ataagaatca agcaaactac tttttccaga gccataattt cccgtgatcg
     1621 ctacattttt taactcatca tcttggattg caaaatctag tgcttgttta aagcttggat
     1681 tttccctttc caaatctata tttttattag aggtcagtct attaaaactt atcccttcca
     1741 cattaggagt tctatttttg aactgaagga atattttttg accccagtta ctgattttac
     1801 gatgtaaact ctccatgtca ttttccttta cagatatatt tatttttatt atatcacaaa
     1861 atcccactct aatttggtgg gacccgaaaa cttcacttta attttaaatc ttacttttta
     1921 cacttgaaaa accaccgctg gcttgtttct gtcggtgctt catcttcaaa gtccgcaaag
     1981 acttccacag atgcaaaacc agcttctttc aataattcca cataggtttc aatcggataa
     2041 gtccgctctt catgcacttc atcccgacgg ataaaccgct cctcttgact ggcttcatct
     2101 ttgacaaaga aggtcaattc gtgaacaatc gaatgtgcat gctccccctc ataggtgtcc
     2161 cagacaaagg caaaatcctc gtagttttca tggtagctat agcctgcaaa gacttcctcc
     2221 atttgataga tggagtgaac gtcaaaaagg aaagtaccac cttgattgag aaccgagtga
     2281 acgccttcaa agacccgcaa aacggcttct cggtccgaca tatagcacag actgtccgaa
     2341 taacaggtca ccgcatcgaa atctcccaca ccttccagag ccatcatatc tgcttgggcc
     2401 aattccatca tgacaccagc agcttctgct tttttctggg ccaattccaa catttcatag
     2461 gacaggtcaa gccctgtcac ctcatagccc ttttgagcaa aacgtacagc ctgaatccct
     2521 gtcccacagg ccaattctaa aattgtcttg gtgtcttgtg gcaagttccg caagctaaaa
     2581 tctgtccatt tatcatacaa gctgtcatcc atgatttcat catagaccgc agcaaatgtt
     2641 tcataagttg ccatattttc ccatttttcc tttcataaaa agggagcagg aaagaactcc
     2701 tcaattccaa agagttcgtt ttcctgcccc cgcacagttg attaggtcag atttggagtg
     2761 ggaagcacga acaaagctgc caatcaacca ctgcgctgag atgttgactt aagtttcaaa
     2821 tagtgcaccc aaatcaaaat ctatcttcat attttttcaa gaagttcatc acttctaatt
     2881 acataatagt gaactctctt tggtcacttt cgagttttat ggtgaccagc tgaaacagtt
     2941 ccccaaactg tttcactatt cctcttctaa aaagtctgcg acttccaaga agctgccttc
     3001 gtgccataat ttttcaaggt tgtagaggtc acgcatttct tctgagaaga tatgaacgac
     3061 tacgcctcca agatccaata ggacccagcc accgttacta tcgccttcta cacggctact
     3121 cttgattcca acatccgcta ccttttcacg gatgttttcc gcaatagcct ccaactggcg
     3181 actgttcatg gagctggcaa tcacaaagta gtctgtcagg ctggtcacgc cttgaacatc
     3241 aattacaaca aggtcctcag ctcgcttgtc atcagcagcc tgaaccacaa cttttactaa
     3301 atctagttct ttcatcttat cctctaatta tattttctaa tctatccacc tgggcaaaat
     3361 tccatgacca gatgttttgg tagtggtcaa tcaagtcttc ggcgtccaat ttacctgaat
     3421 aacctgtcgt cgttccgccc tggatcacag ctaccttgta gcctagttca aaggctactt
     3481 taatcgtcgt atcaatgcaa aaattggtcg ccatgcccat gataatgagt cgctcaattt
     3541 ccttttcctg caaataagca tgaagccccg tttccttgaa ggcactgtta aaggacttat
     3601 cgaaaatctt ctcactagct aagggtgcaa caattgagtg aatctcccag tcaagcagtc
     3661 cccttgacca attcctgatc atgatggcgg acatggatga cttctagccc cgcttgacgg
     3721 gcttgtgcaa ggctatctta ccagagggct aatcgctctt caatggcata aggtttttcc
     3781 tccactagaa ggttctgaat gtcaatgact aacaatgcag atttcatagc tgaacaccag
     3841 gcgtttccaa acggaagacc aattctcttt cttcttcact atccaagaca aaattatttt
     3901 tctccaagag atgagccatt ttctgattgc ccttaacata ggctgcaatc aaatggtcgc
     3961 agacaggatg tgattgagcc attgctatta cgacttccaa agccgcctgg ccatagcccc
     4021 ttccttgaaa ctcttggcca atcataaatc gccagagcat atactgctgg atgtcttcat
     4081 caatgtcaat caaggcaaaa ccaaccacct gttcatccgc ccagatggca taaggggcaa
     4141 ggttctagtg caagtacact tctcaaagtt ctagtcaaca tctcagcgca gtggttgatt
     4201 ggcagctttg ttcgtgctac acactccaaa tctggcctct acgactgatg cgaacagagt
     4261 tcgcttcatc tccaacctca aactgtctcc cagacagttt gagctgtgcg gggggtggga
     4321 gtgaaacagt ctggggatag actgtttcag ctcaacaact ggaaataagg acttgttgac
     4381 gaactctttt agaacccatc gagttctttc ccactcccat tctctctata taaccaggca
     4441 tccgccaagg aacgcacgtt tggagccacg aagcccttgt cctcctcggc tactgtcaag
     4501 tctaagactg cttgatagga acgttcatca agatatttta tagcctttta gtttactttt
     4561 agtactaggc aatgagctgc aggctgtact ggagtacggc aaagcgagtt aacgaagtaa
     4621 taaaagaaaa actaaatgac tataatgtaa tcatccctac tccttcaacc aaacaacata
     4681 gccattatag gtttccaaaa tttgtggata aatgggaatc cccttcttgg ctaagtagga
     4741 aatggtttgg gcagtttcat aggctactgc cttgtccaag gactcttttg cgatacgacg
     4801 cgcctcgtcc accccaggaa aatcccgatt gggttcgatg tagtccgcta catagagaac
     4861 cttgtccagc aaggtcatct gccccgcccc aacggtgtgt cgttgaatgg cttggaaaat
     4921 ctcctcatct tccaaaccaa aatcttctgc gattttatag gcgccaacca caccgtgcca
     4981 gatgttatta tcccagttga gcaggtcctt gtccaaatca tacttggcaa ttaagtccaa
     5041 aaaaacttgg tcttccactt ccttagcata gtcatgcaag agtgctgcta gactggcttt
     5101 tttcggatca cagccatatt ggtctgccaa gacaagcgcc gcctgctcca cacccaaaac
     5161 atgttggaag cgcgctggct tcatggctac acggattttt tccaaaagag cctgacgatc
     5221 aaatgtcaaa tctgctgcaa tcattggtaa agtccttttt cttcgatata gtccagaact
     5281 tcatgaggca ccataaaatt cggcacccgt ccttgcttga taaaactacg caccatgctg
     5341 gaggaaatgt ccatcaaggg cacatctacc cagataaccg gataggacgt ccctgccttg
     5401 taacgagggc gctggacacc aacaaactgc accaactgga ccaattcatc aatcctatgc
     5461 cattttggaa ggtagtctac catatctgca ccgatgatga aataataatc cgtatcagga
     5521 tttttttcct tcagcaattt catggtgtcg taggtatagc tgacaccacg gcgttctagt
     5581 tcaatggttt caatccccaa gccctcaata cctgcaatag ccatcttgag catggaatag
     5641 cgatggtatt cgtcaatggt ttctttctta tccacatgag gtggaatgaa ttctggcatg
     5701 agcaagactt gatccagctt caactgttga cggacctggt ctgccacaat caagtgagca
     5761 ttgtggacag ggttgaaatt ccctcccaga atccccactt gtttgcgatt ggtttctttc
     5821 ttttcaacct ctaattcaac cttggtaaag ggtgttaaga gttcaatagc cataggctct
     5881 ccttcttaga tttctttgac ctgtttggaa attttacggt tctctttctt gctagattgt
     5941 ttgaataaaa tcaagatgcg accgattttt tggaccgtat ccacaccgat ttcttcttcc
     6001 aagatttcag ctacttcgtg gatgttttca tccgtatttt gtagcaaggt caccttgatc
     6061 aattcacgcg catccagtgc ctgacgaaca ctggttttaa tctggtcatt tagaccattt
     6121 ttcccaatct gaatgatggg tttgagactg tgtgcctgac tgttcaaaaa ggcacgttgt
     6181 tttgaagtta gcataattta ttatacacaa agggcgttgt aacagactgg gagactgtta
     6241 caatcgggaa ataaagaaaa caaagttttt ccttattgcg ccgatatact tagcccgtgt
     6301 tcaaatcaga agacttgaac ttcctttctc tttaattgta aaaactgaaa tcaaatacct
     6361 cttatactca atgaaaatta aaatcagact aggcgacgca gatgcagata gaactggagt
     6421 tcatcaaatc aagtcaacaa cgtctgcttt taattttcga agagtattag ataatcgcct
     6481 tgcgaatgac cacatccaca cccttaggag cccaggcagc aatcttggct ttttcattga
     6541 cacgaatcca accaagtcct gagaagacca catccgtctt ctcgttgatg gtaaattcat
     6601 gacggaccag ttcaggaaat tctttcaatt ccttgctggt tggtggcact aaaagtgagc
     6661 ccgcatgttt ctgataaaac tcgctagctc cctgcaactt ggtgcggtgg agttggagct
     6721 cattatcaaa gaaggccgta aagccttggc gttcaccagc cacaaagtca aatcgtccca
     6781 gaccagccaa gaacaaggtt tgttctggat tgagctggta ggtctttggc ttgatttcct
     6841 tgcgagggct gatgtacttg aggtttttag ccgtcaaata atgggccatc tggtgacggt
     6901 ggataattcc tggcgtgtca tagatgtatg aaccatcatc cagagggatt tcaatcttat
     6961 ccagcgtcgt tccagggaaa cgtgaagtcg taatgacatc cttgtcccct gtgatttctt
     7021 gaataatagc gttaatcaag gttgacttgc cgacattggt cacgccgacc acatagacat
     7081 cccgtccctt gcggtgttgt tcaatctttt caatcaaatc cttgatagcc tgcttatttt
     7141 gggctgaagt caagaccaca tcaaccggtc gcatgccgat ttcatgtgcc cgctcggtca
     7201 accactgagt aaccttgcct gtcttgacag acttgggtaa aatgtcctgc ttgttgccga
     7261 ccaaaagcac gtcatttcca gaaataaagc gaggtaaacc tggaatgact gacccattga
     7321 aatcaaaaat atcaatgaca ttaaccacca aggcatcgct ttctcctacg ctatggagga
     7381 gtttcaagaa atcatcgtct gagatattga catctgaaat ctcattgtaa tggcgcaaac
     7441 ggaaacaacg ttggcagtag agttgccctg tttccaggcc tttttctagg gctgattgag
     7501 gtgtaaaacc agcgacagcc ttgtctagcg tctgaatctg ggcaccacag ccgatacaaa
     7561 ataattcttc cacttacatc tccctcttgt agtctatcgc tccatattta gcgatgattt
     7621 ttttcatagt ccgtcgctca cgccaacggt taatctgcgt attgatagag tccgtcttta
     7681 tcaagggttt gaccaagaca gacttgagac cagctcgctt agcagcccga atatccgtca
     7741 tcagctgatc gccaatcata attacctcat acggctggac atcaaagcgt ttcaaagcac
     7801 ggttaatccc aaaggtgaaa ggcttgagag cgaaggcttc aaattcaatc ccaaattttt
     7861 caaccgcccg tttgacacgt tcgtatttat tgttagacac caccacaaca ggaatacctg
     7921 cgtcctgcaa atcatgtaac cactggcgca tctctggcgt accatcggga ttattccaag
     7981 caatcaaggt attatccaag tcaacaaaca ctacttttat gccatgtttt ttcaagcttt
     8041 cgacggtcac gtcataagcc ttttccaagg caaaatccgg catgtaattt tctaaactca
     8101 ctgtcgtctc caaagttttt tatcagataa ttataccatt tttctaagaa aaatgcgaat
     8161 cgatagcaga aaacactcgg atcaaccgag tgccttctgg aggtaatgta atggtagttg
     8221 tgaattaaat ctgaccttct tccaaaatcc aatctttcgg aatgaaaatc atatgggtta
     8281 gcaatcaatc aatatcaaga gtaaattttg aaaaagcaaa accgatttgc tctactcctg
     8341 actcatcctc atccaacccc aaaatggtaa gagcatgtcc ataatctgca tgactatcat
     8401 cgaatagacg ccacgcgaga gcgcgataaa ttaacttctt agcatcatag agactgtaac
     8461 gctgtctgta ctctgaaaat gattgacaat ccttaccttt cggttctact cctttggttt
     8521 tagcaatggc aatcaagcta tcataattat gataacgcaa agtactattc aaggattggg
     8581 ctaaacgaac agcatagtcc aaagctaaat cactcacctc aacagccaca gttctcaatt
     8641 ctttacgtag gggattcaat agagacgcag cagtttctag tctgtatacc ggactttaac
     8701 aaacaaaaat gaaaacagcc gaacatgtcg actgcttccg tggaga
//
LOCUS       DBNFCK010000076         8708 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.076, whole genome shotgun sequence.
ACCESSION   DBNFCK010000076 DBNFCK010000000
VERSION     DBNFCK010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8708
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.076"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            179..1666
                     /locus_tag="AC1PIW_001372"
     CDS             179..1666
                     /locus_tag="AC1PIW_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HIP6606302.1"
                     /translation="MKKIAFDSTKYLNLQRDHILERIAQFEGKLYMEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKDQVEIVIAINASNIEHSKARGDLGISYDQEVFRLIDTF
                     NDIDIYVGSVVITQYRNQPAADAFRKQLEKHGIKSYLHYPIKGYPSDIDHIISPEGMG
                     KNDYIETSRNLVVVTAPGPGSGKLATCISQLYHDQLHGVTSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDLNMIDPFHLQTYGKTAVNYNRDIEVFPVLNRTFERILNKSPYA
                     SPTDMGVNMVGYSIVDEEAAIEASKQEIIRRYYQTLVDFKAERVSEQAVKKIELLMNE
                     VGVTPADRKVVIAAREKAELTTSPALAIQLPTGEIVTGKTSDLLKPTATVLLNAIKQI
                     ANIDDETLLIEPNYIRPIQELKADYLDKTNTRLDASEILNALAITAQDSPLAARAMKE
                     LGQLNGSEAHSTVILSDEDKSVLRKLGINLTFDPIYQHNKFYQKN"
     gene            complement(1748..3481)
                     /gene="ptsP"
                     /locus_tag="AC1PIW_001373"
     CDS             complement(1748..3481)
                     /gene="ptsP"
                     /locus_tag="AC1PIW_001373"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HIP6606303.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETVTVEDTNAEEARL
                     DAALEASQNELSVIRENAVASLGEEAAAVFDAHLMVLADPEMIGQIKETIRAKKTNAE
                     TGLKEVTDMFIAIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVRLPNPATIDEESIV
                     IAHDLTPSDTAQLNKQLVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     VLAVNGITGDVVINPSEEVIAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNDNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDLPHEMNPFLGFRALRISISETGNQMFRTQLRALLRSSVHGKLRI
                     MFPMVALLTEFRAAKAILDEEKANLLAEGVAVADDIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYAHRALTECATAEEV
                     LELQKEYVDFD"
     gene            complement(3486..3749)
                     /locus_tag="AC1PIW_001374"
     CDS             complement(3486..3749)
                     /locus_tag="AC1PIW_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HIP6606304.1"
                     /translation="MASKDFHIVAETGIHARPATLLVQTASKFASDITLNYKEKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAITETMEKEGLA"
     gene            complement(3905..5086)
                     /gene="icd"
                     /locus_tag="AC1PIW_001375"
     CDS             complement(3905..5086)
                     /gene="icd"
                     /locus_tag="AC1PIW_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019780541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HIP6606305.1"
                     /translation="MAEKILMREGRLIVPDCPVIPYIEGDGIGRDIWSVSQKVIDAAV
                     KKAYAGQRSIQWREVFAGEKAYELMGTSLPNETLEIIREHLVAIKGPLGTPIGKGHRS
                     LNVALRQELDLYACVRPIRYFKGVPSPLKEPEKTDITIFRENTEDIYAGIEWEAGSDE
                     VAKVISFLQEEMGVVNIRFPESSSIGIKPISEEGSKRLIRSAIEYALEHQLKMVTLVH
                     KGNIQKFTEGGFRKWGYELAKEEFATELAEGTLVVNDIIADNFLQQILLAPENFQVVA
                     LTNLNGDYASDALAAQVGGIGISPGANINYQTGHAIFEATHGTAPDIAGKDLANPCSL
                     LLSACMMLEYIGWKEAAVLIIQALEDAFINKNVTLDFAKILDVAHQSTSQFSEVLIDS
                     I"
     gene            complement(5089..6207)
                     /locus_tag="AC1PIW_001376"
     CDS             complement(5089..6207)
                     /locus_tag="AC1PIW_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HIP6606306.1"
                     /translation="MTETNGLKDAIACDTRISAIENDRLSYAGYDIAELMENNASFEE
                     VIYLLWNLHLPTQIELNYFEKSLRKEYAISDAVEQCILIQSRRHLHPMSVLRSTVSLL
                     GVYNVHAEENSLEALYEQSIQIMAKVPTIIATFARLRRGLVPIEPRSDLGFAANFLYM
                     LNGEEPSDLQIKAFNKALVLHADHELNASTFAARVTASTLTDLYSCVTTAIGTLKGSL
                     HGGANERVFDMLLAIRESGDTERYLQKKLDSKEKIMGFGHRVYKTVDPRQEYLKEMAR
                     DLTFGTDDEAFYRLSEEVESFIKTKKGLIPNVDFYSATVYHVLGIDSDIFTLIFAMSR
                     MAGWIAHVQEQRKNNKLIRPRSSYLGGRNLVYIPIGKR"
     gene            complement(6204..>8708)
                     /gene="acnA"
                     /locus_tag="AC1PIW_001377"
     CDS             complement(6204..>8708)
                     /gene="acnA"
                     /locus_tag="AC1PIW_001377"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HIP6606307.1"
                     /translation="TKNHIMELLHYQAASPKGEIPFKPSRVILQDFTGVPVVVDLASM
                     RDAVVKAGGNPELINPEIPVDLVIDHSVQVDFFGTEDALEKNIALEFERNNERYEFLK
                     WAENSFENYRAVPPATGIIHQVNIEFLSDVIINKDGLLYPDSMFGTDSHTTMINGIGV
                     LGWGVGGIEAEAAMLGEASYFPVPEVIGVRLDGQLPKVATATDLALKVTQLLRQENVV
                     GKFVEFFGPGLSSLTLADRATVSNMAPEYGATCGYFPIDGETLHYMRLTNRSEEHVEL
                     TEAYAKANYLFYDAERFPSYSKVLELDLSTVVPSISGPKRPQDLIELTDAKAEFQASL
                     IREVGVRGFGLEEAELDKTASVKYVEGDEQIQTGHVAIAAITSCTNTSNPYVLLAAGL
                     LAKNAVEKGLAVSKTVKTSLAPGSKVVTGYLKKSGLQTYLDTLGFNLVGYGCTTCIGN
                     SGDLCPEVAEAIKEEDLLVSAVLSGNRNFEGRINPLVKANFLASPPIVVAYAIAGNMN
                     VDLTRDPLGYDEKQQAVYLADIMPSREEVDDYIERYVTRDLYKEEYQQVFTDSQAWNA
                     IETKINKNYNWNSSSTYIQNPPYFDNMQADLSIKPLENLSVLAKFGDSVTTDHISPAG
                     NIARLSPAARYLEENGIVYKDFNSYGSRRGNHEVMIRGTFANIRIKNELADGKIGGWT
                     RVGEEILPIYDAAMRYKEVGVGSIVIAGKDYGMGSSRDWAAKGSSLLGVKAVLAESFE
                     RIHRSNLVMMGVLPLQFLEGQSAESLGLTGHESYTIDLPEDVGVGQIVTVHAQTDDVT
                     KEFQALVRFDAEADIRYYRHGGILPMVVRKKLGGKV"
BASE COUNT         2559 a         2112 c         1540 g         2497 t
ORIGIN      
        1 atttttttaa acacttgttt aatttttctt ctgtctgact ttttcttgac aagattagca
       61 cagtttgtta tagtattgtt cgggcacctc ttttgagagg tcggagaaaa ggctccctag
      121 ttttagggag ctattttttg ttttcccaga gattaataca catttggagg acatatttat
      181 gaaaaaaatc gcatttgatt caactaaata tttgaatttg caacgtgacc atattttaga
      241 gcgtattgcc caatttgaag gcaagctcta tatggaattt ggcggaaaaa tgttggaaga
      301 tttccacgca gcccgtgttt tacctggcta tgaaccagat aacaaaatca aactcctcca
      361 agagttaaaa gaccaagtag aaatcgtcat cgccatcaat gcaagcaaca tcgaacattc
      421 taaggcacgt ggtgacttgg gtatttctta tgaccaagaa gtcttccgct tgattgacac
      481 cttcaatgat attgatattt atgttggttc tgtggtcatt acccaatacc gcaaccaacc
      541 agccgcagac gccttccgca aacagttgga aaaacatggt atcaagtcct atctccacta
      601 tccaatcaag ggttatcctt ctgatatcga ccacatcatt tcaccagaag gtatgggcaa
      661 aaatgactac attgaaacta gtcgcaatct cgtcgttgtc accgcacctg gacctggttc
      721 aggtaaattg gcgacctgta tctcccaact ttaccacgac caattgcatg gagtaacatc
      781 aggctatgcc aagtttgaaa ccttcccagt gtggaactta ccactccacc atcccgttaa
      841 cttggcctat gaagcagcaa ctgccgacct ggacgacctc aacatgattg accccttcca
      901 tctgcaaact tacggcaaaa ctgcggtcaa ctacaaccgt gacatcgagg tcttccctgt
      961 cctcaaccgt acctttgaac gtattttgaa caaatcacca tacgcctcac cgacagacat
     1021 gggcgttaac atggtgggct attccatcgt ggacgaagag gctgcgatcg aagcatccaa
     1081 gcaagaaatc atccgccgct actaccagac cttggttgat ttcaaagcag agcgggtgag
     1141 cgagcaagca gttaaaaaga ttgaactcct catgaatgaa gtcggtgtga cgccagcaga
     1201 tcgtaaggtt gttatcgctg cgcgcgaaaa agcagagctg acaactagcc ctgcccttgc
     1261 tattcagttg ccaactggag agattgtaac aggtaagaca tccgacctcc tcaagccaac
     1321 tgcaactgtc cttctcaatg ccatcaagca aattgctaac atcgatgatg aaacacttct
     1381 catcgaacca aactacatcc gtccaattca agaattgaag gcagattatc tggataagac
     1441 caatacacga ctcgatgcaa gtgagattct taacgccctt gccatcactg cacaagatag
     1501 ccctcttgct gcacgtgcca tgaaagaatt aggccaatta aatggtagcg aagcccactc
     1561 gactgttatc ctatctgatg aagacaagag tgtccttcgt aaattaggca tcaatcttac
     1621 ctttgaccct atctaccaac acaataaatt ttatcagaaa aactaagcac acaaaaagac
     1681 ttgctccgac aagtcttttt gtgaacagta aaaaagcaag gattcaattc cctgcttttt
     1741 tcaacgatta atcgaaatca acatactctt tttgaagttc aagaacttct tctgccgttg
     1801 cacattctgt caaggcacgg tgagcatatt cttccatctt agctgtatca agtttcttca
     1861 taaggctacg tgtacgaagt acagatgttg cactcataga gaactcatcc aagcccatac
     1921 ctacaagaag tggaacagct tgttgatcac cagccatctc accacacata ccagcccatt
     1981 taccttctgc gtgtgcagcc ttgataacat tgttaatcaa gcgaaggatt gatgggttat
     2041 atggttggta gaggtatgaa acttgttcat tcatacggtc tgcagccatt gtgtactgga
     2101 taaggtcgtt cgtaccgatt gagaagaagt caacttcttt ggcaaattgg tctgcaagca
     2161 ttgctgctgc agggatttca atcatgatac caacttggat atcatccgca actgctacac
     2221 cttcagccaa caagtttgct ttttcttcat caaggattgc ttttgctgca cggaattcag
     2281 tcaacaaggc aaccattggg aacatgatac gaagtttacc atgtactgat gaacgaagaa
     2341 gggcacgcaa ctgagtgcgg aacatttggt taccagtctc agagatagag atacgaaggg
     2401 cacggaaacc aaggaatggg ttcatttcgt gtggaagatc gaagtaagga agttccttat
     2461 caccaccaat atccattgta cgaaccacaa caggcttgcc attcatacct tcaagaacag
     2521 ccttgtaagc ttcatattgc tcgtcttcag ttgggaagtc ttgtgagtcc atatacaaga
     2581 actctgtacg gtaaagacca actgcttctg caccgttatc atttacacct tcaacgtctt
     2641 ttggtgtacc aatatttgct gccaattcaa agtgtttgcc gtcagctgta actgtttgag
     2701 catctttcaa aagagcccat tcagcttttt gtttagcata agcttcacca gctgctttaa
     2761 attctgcaat cacttcttca gaagggttaa tcactacgtc acctgtgata ccgttaacag
     2821 ccaatacatc accgtcttta acgatttcag taatgttatt tgtccccaaa acagcagcaa
     2881 tttcaagtgt acgagccatg atagctgagt gacttgtacg accaccaatg ttcgttacga
     2941 aagcttttac caactgtttg tttaattgcg ctgtatctga aggtgtcaag tcatgtgcga
     3001 taacgattga ctcttcgtcg atagttgctg ggtttggcaa gcgaacacca agcaagtgtg
     3061 ccaatacacg tttggctacg tcacgaatat ctgcggcacg ctcttgcatg tatgggttat
     3121 cttccatacc ttcaaagata gcgataaaca tatccgtaac ttctttcaaa ccagtctcag
     3181 cgtttgtttt cttcgcacga attgtttcct taatctgacc aatcatttct ggatcagcaa
     3241 gaaccatcaa gtgtgcatca aatacggctg cggcctcttc acctaggcta gctactgcgt
     3301 tctcacgaat aacagaaagc tcgttttgag atgcttctag agcagcatcc aaacgagctt
     3361 cctctgcatt tgtatcttca actgtaacag tttcgaagga caaatctggc tgaacgagta
     3421 gatatgcctt agcaacagca acaccatctg atgctgcgat tcctttaagc atttctgtca
     3481 tattcttatg ccaatccttc tttttccatt gtttcagtga tagctgcgat tgcgtcgtca
     3541 gcatcagcac cttcagcaga gattgttacg tcagcgcctt gaccaacacc aagactcata
     3601 acacccatga tagatttaag gttaacagac ttttctttgt agttcaaagt gatgtctgaa
     3661 gcaaatttac tagctgtttg cacaagcaaa gttgctggac gtgcgtggat acctgtttct
     3721 gccacaatgt ggaagtcttt tgaagccata tttggattct cctttatttt tacaaaattg
     3781 cgagttattt gtgataaccc ttacaaagga cattatacca ctttttgaaa tcattttcaa
     3841 gattttttta cctttctttc atgtttccgt cataaaaaga gaggttcatt ttactttttc
     3901 ttcattaaat agaatctatt agaacttctg aaaattgact agtagattga tgagcaacgt
     3961 ccaaaatttt agcaaaatca agcgtaacat ttttatttat aaaagcgtct tccaaggcct
     4021 gaataattaa aacagccgct tctttccagc caatatattc caacatcata caagccgaaa
     4081 gcaagagaga acatggatta gccaaatcct ttcctgcaat atcaggagca gttccatgtg
     4141 tcgcttcaaa gatagcatga cctgtctgat aattaatgtt agcacccggt gagatgccta
     4201 ttcctccgac ctgagccgca agagcatctg atgcataatc tccatttaga tttgtaagag
     4261 caaccacctg gaagttttcc ggcgccaata aaatctgctg cagaaagtta tccgcaataa
     4321 tgtcatttac taccaaagtc ccctctgcaa gttctgtagc aaattcttct ttcgccaatt
     4381 cataacccca cttgcggaag ccaccttctg taaacttttg aatatttccc ttatgaacta
     4441 aggtcaccat cttcaattga tgttctaggg catattctat tgcagagcga atgaggcgtt
     4501 tactcccttc ttccgaaatc ggtttgatac caatacttga agattctgga aaacggatat
     4561 ttaccactcc catttcttct tgcagaaagg aaatgacttt ggcaacttca tctgaaccag
     4621 cctcccattc aatacctgca taaatatctt ctgtattttc acgaaaaata gtaatatccg
     4681 tcttttcagg ctctttcagc ggacttggca ctcccttaaa atagcgaatc ggacgcacac
     4741 aggcatacaa atctaattct tgacgaagtg caacattaag agaacgatga cctttcccaa
     4801 tcggcgtccc caagggacct ttgattgcaa ctaaatgctc acgaattatt tccagtgtct
     4861 cattcggcaa agaagttccc attaattcat aagccttttc tcctgcaaaa acctccctcc
     4921 actgaataga acgttgacct gcataggctt ttttcactgc agcatcaata actttttgac
     4981 tgactgacca aatatccctg ccaataccat ctccttcaat atagggaata acgggacaat
     5041 ctgggacaat taaccgtccc tctctcatta ggattttttc tgccatattt acctctttcc
     5101 aatcgggatg taaaccaaat tcctaccacc taagtaggaa gagcgcggac gaatcaattt
     5161 attatttttc cgttgttcct ggacatgtgc aatccacccc gccatacggc tcattgcaaa
     5221 aatcaatgta aaaatatcac tatcaatacc tagcacatgg taaactgtcg ccgaataaaa
     5281 atctacgttt ggaattaaac cttttttggt tttaataaag ctttctactt cctctgacaa
     5341 tctataaaac gcttcatcgt ctgttccgaa tgtcaaatca cgcgccatct ccttcaaata
     5401 ctcttggcga ggatcaaccg ttttatagac tcgatggcca aatcccataa ttttttcctt
     5461 cgaatccagc ttcttctgca aatatctctc ggtatcacca ctctcacgaa tagccaacaa
     5521 catgtcaaac actcgttcat ttgcaccacc atgcaatgag cctttcagag tcccaattgc
     5581 cgtggtgaca caagaatata aatccgttaa cgttgatgcc gtgacacgcg cagcaaaagt
     5641 agatgcattc aattcatgat cagcatgaag caccaaagct ttgttaaacg ctttgatttg
     5701 taaatcactc ggctcctcac cattcagcat ataaagaaaa ttcgccgcaa aacccagatc
     5761 agaccgcggt tcaattggaa ccaatccgcg tctcaaccga gcaaatgttg caataatcgt
     5821 cggaaccttg gccataatct gaatagattg ttcatacaaa gcctctaacg aattctcttc
     5881 agcgtgtaca ttatatactc caaggagaga tactgtcgaa cgcaacacgc tcattggatg
     5941 caaatgtcgc cgtgattgaa tcagaataca ttgctctacc gcatctgaaa tcgcatattc
     6001 ttttcgcagg cttttctcaa aataatttaa ttcaatctga gttggcaaat gcaggttcca
     6061 cagaagatag atgacttcct caaaagaagc gttattctcc atcagttcag cgatgtcata
     6121 ccctgcatag gacaagcggt cattttcaat tgcactgatt cgcgtatcac aggcaatcgc
     6181 atcttttaac ccattcgttt ctgtcatacc tttcctccta atttctttct tactaccata
     6241 ggtaaaatac ctccgtggcg ataataacga atatccgctt ctgcatcaaa ccgcaccaga
     6301 gcttgaaact cctttgtaac atcgtctgtt tgcgcatgga ctgttacaat ttgaccaaca
     6361 ccgacatctt ctggcaaatc aattgtatag ctttcatgtc cagtcaagcc aagactttct
     6421 gccgactgac cttctaaaaa ttgtaaagga agaacaccca tcatcactaa gtttgaacgg
     6481 tgaatccgct caaatgactc agcaagaaca gccttcacac caagtaaact tgaacctttc
     6541 gctgcccagt cacggcttga ccccatccca tagtctttac cagcaataac aatactaccg
     6601 acaccaactt ccttatatct catagccgca tcatagatag gaagaatctc ttcgccaact
     6661 cttgtccaac caccaatttt tccatctgcc agctcattct ttatacgaat atttgcaaaa
     6721 gtcccacgta tcatgacttc gtgatttcca cgacgacttc cataagaatt aaaatcctta
     6781 taaacaatgc cattctcttc gagataacga gcggctggac tcaatcgtgc aatattccct
     6841 gccggggaaa tatgatctgt cgttacagaa tcgccaaatt tagccaaaac agataaattt
     6901 tccagtggtt ttatggataa atctgcctgc atgttgtcaa aatagggcgg attttgaata
     6961 taggtagaag atgaattcca attgtaattc ttatttattt ttgtttcaat cgcattccaa
     7021 gcctgactat ctgtaaatac ctgttggtat tcttccttat aaaggtcacg agtcacataa
     7081 cgctcaatgt agtcgtctac ctcctcacga cttggcataa tgtccgcaag gtagacagct
     7141 tgttgcttct catcataccc cagcggatcc cttgttaaat ctacattcat atttcccgca
     7201 atagcatagg caactacaat cggtggactc gctaagaaat tagcttttac caacggatta
     7261 atccgtcctt caaagttacg atttccagat aataccgcag aaaccaataa atcttcttcc
     7321 ttaatcgcct ccgccacttc aggacacaga tcacctgagt taccgataca agttgtacaa
     7381 ccataaccaa ccaaattgaa ccctaaggta tctagatagg tctgcaaacc tgattttttc
     7441 aaatagccag tcactacttt tgagcctggt gccaatgacg tcttgaccgt cttagaaact
     7501 gccaaacctt tttctaccgc atttttcgcc agtaatcctg ccgccaaaag gacatacgga
     7561 ttggaggtat ttgtacacga ggtaatcgcc gcaatagcaa catgacctgt ttgaatttgc
     7621 tcatctcctt caacatactt aaccgaggct gttttatcta actctgcttc ctccaaacca
     7681 aatccacgca cgcctacttc acgtattaaa ctagcctgaa actctgcttt tgcatctgtt
     7741 aattctatca aatcctgtgg tcttttagga ccagaaatag atgggactac tgtcgataaa
     7801 tccaattcta aaaccttgct ataagacgga aaacgttcag catcatagaa taaataatta
     7861 gccttagcat aggcttctgt caactccacg tgctcctccg aacggttggt caatctcata
     7921 taatgtaaag tttctccatc gatcgggaaa tagccacaag tcgcaccgta ttctggagcc
     7981 atattggaaa cagttgctcg atccgctaat gtaagagaag ataaacctgg tccaaaaaat
     8041 tcgacaaact tcccaactac attttcctga cgtagaagtt gagtgacctt aagcgccaaa
     8101 tccgttgcag tggcaacctt aggcaattgc ccatctaggc gaacaccaat cacctcagga
     8161 acgggaaaat aagatgcttc acccaacatt gcagcttccg cctcaatacc acctacaccc
     8221 caaccaagta caccaatacc attaatcatg gtcgtatgac tgtctgtacc aaacatagaa
     8281 tctggataaa gcaaaccatc cttgttgata ataacatcgc ttagaaattc aatgtttacc
     8341 tgatggataa ttcctgttgc tggaggcaca gcgcgataat tttcaaagga attctctgcc
     8401 catttcagaa attcatagcg ttcattattt ctttcgaact ctaaggcaat atttttctcc
     8461 agagcatctt cagtcccaaa aaaatcaact tgaacagagt ggtcgatgac taaatccact
     8521 ggaatttctg ggttaatcaa ttcagggttt ccacctgctt tcaccaccgc atcacgcatg
     8581 gaggctaaat ctaccacaac aggaacacct gtaaaatctt gcaaaataac acgactaggt
     8641 ttaaaaggaa tttccccttt aggagaggca gcctgataat gaagcaactc cattatgtga
     8701 ttttttgt
//
LOCUS       DBNFCK010000077         8697 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.077, whole genome shotgun sequence.
ACCESSION   DBNFCK010000077 DBNFCK010000000
VERSION     DBNFCK010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8697
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.077"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..1064)
                     /locus_tag="AC1PIW_001378"
     CDS             complement(<1..1064)
                     /locus_tag="AC1PIW_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HIP6606308.1"
                     /translation="MKKETSKFTKKVILGQLLICTSFLVWAGQTVAAEEVSQGEPVAI
                     NNETPPVIEAKPLVEATPSTETEPSTPEEKQEEPQVAGKNVVEGKKPVTNSQHQMVSE
                     LSTVPSVSIKNPDAATDGSAYGSSDDANSNTKIIAGKETGEPDNGYSKWDHVYVQYDF
                     EKEVPVHTVKIHRNTYDNAVSTFKDVKVELSTSADFSPETTEVIFKQADVEETIANKG
                     QPQVIQLPAPINARYIRIWGRGHYIQNTNSSWKGYSNGVLFNEIEVLAQVEADAGTNE
                     EPQTELVNLAKLKKPYFYGKAPTNPEAINDGKFDENYTTQESIGDKAYLQYEFRNVYT
                     IEEIKVQLAPGEYRNFRIDL"
     gene            complement(1435..2775)
                     /locus_tag="AC1PIW_001379"
     CDS             complement(1435..2775)
                     /locus_tag="AC1PIW_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HIP6606309.1"
                     /translation="MRTSFIQSSHQEACIQAIQELVAFPSVLQEHQADTPFGQAIQDV
                     LEHTLALTEKMGFKTYLDPAGYYGYAEIGQGEELLAILCHLDVVPAGDLSQWQTPPFE
                     AVVEGDYIIGRGVQDDKGPSMAALFAVKALLDAGVQFNKRIRFIFGTDEETLWRCMNR
                     YNQLEEVATMGFAPDSSFPLTYAEKGLLQAKLHGPGHPCLSIEAGTAYNVVPAKASYS
                     GHLLAGVIAELDQLGFDYEIKDDQVTVLGISRHAKDAAEGVNAIVRLAKALEHFENHP
                     ALDFIVNAVGEDATGFKLFGDVTDEPSGTLSFNIAGLTISAKKSEIRLDIRIPVTADK
                     EALVSTLQAKAQSCGLTYEEYDYLASLYVPLDSQLVSTLMSVYQDKTGDLTSEPISSG
                     GATFARTMPNCVAFGACFPDTEQTEHQENERMPLEDLYKTMDIYAEAVYRLTAE"
     gene            complement(2894..4396)
                     /locus_tag="AC1PIW_001380"
     CDS             complement(2894..4396)
                     /locus_tag="AC1PIW_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HIP6606310.1"
                     /translation="MSEKVKKGFKLPSSYTILMLIIAFMAVMTWVIPAGQYQVDEAGR
                     LVAGTYEKVAQNPQGIYDVFMAPVRAMLGHGATEAAINVAFFIIMVGAFLGVVNETGA
                     LDVGIASIVKRFKGREKMLIYILMPLFALGGSTYGMGEETMAFFPLLVPVMMAVGFDS
                     ITGVAIILLGSQIGCLASTVNPFATVVASDAAGVSVADGMIWRFVFFVVILAMGVLFV
                     ANYAEKVKNDPTKSLVYKQREADMQHFNVAATQEVNAELSPAQKRVLWVFVLTFVLMI
                     CSFIPWEDLGVTIFTQFNDWLIGLPFIGQVIGSSTAALGTWYFPEGAMLFGIAGIVVG
                     LVYGMSEERLVKSFMFGAADIFSVALICAIARGIQVIMNDGMITATILHMGEEGLQGL
                     SSQVFIILTYLFYLPMSFLIPSSSGLAGASMGIMAPLGEFVNVPASLVITAFQAASGL
                     LNLVAPTSGIVMGALALGRVEIGTWYKFVGKLIVGIMIASIAILVIATFF"
     gene            complement(4739..5686)
                     /gene="arcC"
                     /locus_tag="AC1PIW_001381"
     CDS             complement(4739..5686)
                     /gene="arcC"
                     /locus_tag="AC1PIW_001381"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HIP6606311.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQKEALVQTAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQNLAADSEKNPAFPLDSLVAMTEGSIGFWLQNALENELLKE
                     GIEKDVASVVTQVIVDKNDPAFENLTKPIGPFYTEEEAKAEAEKTGATFKEDAGRGWR
                     KVVASPKPVGIKEIGTIRTLLNAGEVVVAAGGGGIPVVQEADGTLTGVEAVIDKDFAS
                     QCLAELVDADLFIVLTGVDYVFVNYNKPDQEKLETVTVAELEEYIKQNQFAPGSMLPK
                     VEAAIAFVNNKPQSKAVITSLENLGALIESDSGTIIVKG"
     gene            complement(5804..6817)
                     /gene="argF"
                     /locus_tag="AC1PIW_001382"
     CDS             complement(5804..6817)
                     /gene="argF"
                     /locus_tag="AC1PIW_001382"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIP6606312.1"
                     /translation="MTNVFKGRHFLAEKDFTRAELEWLIDFSAHLKDLKKRNIPHRYL
                     EGKNIALLFEKTSTRTRAAFTVASIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMF
                     DGIEFRGFSQKMVEELAEFSGVPVWNGLTDAWHPTQMLADYLTVKENFGKLEGLTLVY
                     CGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEEEVVALAEGFAKESGARVLITDNA
                     DEAVKGADVLYTDVWVSMGEEDKFAERVALLKPYQVNMELVKKAENENLIFLHCLPAF
                     HDTNTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGDPFV
                     PRV"
     gene            complement(6836..7276)
                     /locus_tag="AC1PIW_001383"
     CDS             complement(6836..7276)
                     /locus_tag="AC1PIW_001383"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6606313.1"
                     /translation="MPSIERTTIGSEEWQRAASIYVRYKVFVLERGIAKEDEFDQNDT
                     EERVYANLFIGEEPVSTGRFLPVGPGQARLTRIATLPNFRGKGYGKMIISSLEAYARE
                     AGFHQLNIHSELTAKTFYESVGYQATSEVYQEDGEWCQTLTKFI"
     gene            complement(7276..8505)
                     /gene="arcA"
                     /locus_tag="AC1PIW_001384"
     CDS             complement(7276..8505)
                     /gene="arcA"
                     /locus_tag="AC1PIW_001384"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HIP6606314.1"
                     /translation="MSNHPIHVFSEIGKLKKVMLHRPGKEIENLMPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGVEVLYLEKLAAESLVTPEIREQFIDEYLEEANIRGRAT
                     KKAIRKLLMSIEDNQELVEKTMAGVQKAELPEIPSEEKGLTDLVESSYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMYSETRNRETLYGKYIFTHHPEYGGKVPLVYNREETTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFERHVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLRVFSVTYENDTLHIEEEHGDLAELLAANLGLEKVELIR
                     CGGGDMVAAGREQWNDGSNTLTIAPGVVVVYKRNTITNAILESKGLRLIKIGGSELVR
                     GRGGPRCMSMPFEREDI"
BASE COUNT         2536 a         1971 c         1605 g         2585 t
ORIGIN      
        1 tccaaatcta tccggaaatt acgatactct ccaggagcta actgaacctt gatttcctca
       61 atagtataga catttctaaa ttcatattga agataagctt tatcccctat tgattcttga
      121 gtagtataat tctcgtcgaa ctttccatcg ttaatggctt ctggattagt tggagcctta
      181 ccatagaaat atggtttttt caatttagcg aggttgacta gctcggtttg tggttcttca
      241 ttcgtaccag catctgcttc aacctgcgcc aagacttcta tctcgttaaa gagaacgcca
      301 ttactatatc ccttccagga actgtttgta ttttggatat aatgcccccg tccccaaata
      361 cgaatataac gagcattgat tggtgctggt aattggatga cttgaggctg tcctttattt
      421 gcaattgttt cttctacatc tgcctgtttg aaaatgactt ctgtggtttc aggagaaaaa
      481 tctgcacttg tagacaattc cactttaaca tctttaaaag tagacactgc attgtcataa
      541 gtattacgat gaatttttac agtatggacg ggaacttcct tttcaaaatc atactgaacg
      601 taaacatgat cccacttgga gtaaccgtta tctggttcgc cagtttcttt accagcaatg
      661 attttggtat tgctattggc atcgtcgcta ctaccgtaag cactcccatc ggtagctgca
      721 tctggatttt ttatagaaac agaagggacc gttgataatt cagaaaccat ctgatgctgg
      781 ctgttggtta ctggcttttt cccttctacc acatttttac cagctacttg tggttcctcc
      841 tgtttttctt ctggagttga aggttctgtt tcagttgatg gagtagcttc tacaagtggt
      901 tttgcttcaa taacaggtgg agtttcattg ttgattgcta ctggttctcc ctgagatacc
      961 tcctctgctg caacagtctg ccctgcccat accaaaaaac tggtacagat gagaagctga
     1021 ccaaggatga cttttttcgt aaacttactt gtttcttttt tcattatttc ctcctataaa
     1081 gtctgcaagt ttatcctacc tatagccaaa ataaagcagt ttgtaaacct atttagtctc
     1141 gtacaagact caaaattcag tttactggtt gatgtgcgat cgcatttctg tcacaaatat
     1201 aacatgtaag cgttttattg tagaatagtc caacttgact tattctatat gcagatgggg
     1261 tctatttcta aaacagcata taataagtca cactttcata tatttttgtc cacttcttgt
     1321 aaacagtgac acatttctct ctcatctttt taatgatatt cttcataaga aaagccctta
     1381 gatatttcac taagagcttt taaactgtaa tttactattc aatgctgata atggctattc
     1441 cgcagttagg cgatagactg cctcagcata aatatccatt gttttatata agtcttctaa
     1501 tggcatgcgt tcattttcct gatgctctgt ttgttctgta tctgggaaac atgcaccaaa
     1561 agccacacag tttggcatag tacgagcaaa ggtagcccca cctgacgaga ttggctcgct
     1621 tgtcaaatct cctgtcttat cttgataaac agacatgagc gtagatacca gttgactgtc
     1681 taatggcaca tacaaggaag caaggtagtc gtactcttca taggtcaagc cacatgattg
     1741 cgctttggct tgtaaagtag aaactagggc ttctttatcc gcagtgacag gaatacgaat
     1801 gtccaagcga atttctgatt tttttgcgct aatggttaga cctgcaatat tgaaactgag
     1861 tgtccctgat ggttcatcgg tcacatcacc aaaaagctta aatcctgtgg catcttcacc
     1921 aactgcattg acgataaagt ccaaggctgg atgattttcg aaatgttcca aagcctttgc
     1981 caaacgaacg atggcattga ccccctcggc agcatccttg gcgtgccgcg aaataccaag
     2041 aaccgttact tgatcatctt tgatttcata atcaaagcct aattgatcta attccgcaat
     2101 aactccggct aataaatgac cggaatagct tgccttggca ggtactacgt tataggcagt
     2161 tccagcttcg atactcagac aagggtgacc tggaccatgc aatttggcct gtagtaatcc
     2221 tttttcagca taagtcaaag ggaaggaaga atctggcgca aagcccatag ttgccacttc
     2281 ttccaactga ttgtagcggt tcatacaccg ccataaggtc tcttcatctg ttccaaaaat
     2341 gaaacgaatc cgtttgttaa attggactcc cgcatcaaga agtgctttta ctgcaaataa
     2401 ggccgccatc gaaggacctt tatcatcctg aaccccgcgt ccaataatat aatctccctc
     2461 taccacagcc tcaaaaggcg gcgtctgcca ttgactaaga tcaccagcag gtaccacatc
     2521 taagtggcaa aggattgcta ataattcttc gccttgccca atttcagcat agccataata
     2581 gccagcaggg tctaaataag ttttaaaacc cattttttcc gtcaaagcca acgtgtgctc
     2641 cagaacatcc tgaatcgctt gaccaaatgg cgtatctgcc tgatgttctt gcaaaacaga
     2701 aggaaaagca accagttctt ggatggcttg aatacatgcc tcctgatgcg atgactgaat
     2761 aaaacttgtt ctcattgtgt cccctttcaa cataataaga tatgatatat tttctagcac
     2821 tattctatca taaaaggaaa aactgagcca aattttatct gtattcgtga taaaatctga
     2881 cccagaattt cgattagaag aatgtcgcaa tgaccaaaat agctatggag gcaatcatga
     2941 tgcctacgat taacttacct acaaatttat accaagtacc aatttcaaca cgaccaagtg
     3001 ccaaggctcc cattacaata cctgatgttg gagctaccaa gttcaagaga cctgatgcag
     3061 cttggaaagc tgtgataacc aagctcgctg gtacatttac aaactcacca agaggtgcca
     3121 tgatacccat agaagcccct gcaagacctg atgaagatgg aatcaagaag gacattggta
     3181 ggtagaagag atatgtcaaa atgatgaata cttgtgaaga caaaccttgc aatccttctt
     3241 cacccatgtg gagaatagtg gcagtaatca taccatcgtt catgatgact tgaataccac
     3301 gcgcgatagc acaaatcaaa gctacgctga aaatatcagc cgcaccgaac atgaaggatt
     3361 ttaccaaacg ttcttcactc ataccataaa ctaaaccaac tacaatccct gcgataccaa
     3421 agagcattgc tccttctggg aagtaccaag taccaagagc agctgttgaa gaaccaatca
     3481 cttgaccgat gaatggaaga ccaatcaacc aatcattaaa ttgtgtaaag atagtcacac
     3541 ccaaatcttc ccatggaata aagctacaaa tcatgagaac aaatgttagt acaaataccc
     3601 agagaacacg tttttgagct ggtgataatt ctgcattcac ttcttgagtt gctgcgacat
     3661 taaagtgttg catgtccgct tcacgttgtt tataaaccaa ggacttagtc ggatcgtttt
     3721 tcactttctc agcatagttg gctacaaaga gaacccccat cgcgagaatg acaacgaaga
     3781 agacaaagcg ccaaatcata ccatccgcaa cacttacacc tgccgcgtca gaggctacaa
     3841 ctgtcgcaaa tgggtttaca gtagaagcca aacagccgat ttgagaacct agcaagatga
     3901 tggctacacc agtaatacta tcaaaaccaa ccgccatcat aacaggaaca aggagcggga
     3961 agaaagccat tgtttcttca cccataccat aggttgagcc tcccaaggca aacaaaggca
     4021 tgaggatgta aatcaacatt ttttcacgac ctttgaagcg ttttacaata gaagcaatcc
     4081 ctacatcaag tgcacctgtt tcgttaacta caccaagaaa ggccccaacc atgatgatga
     4141 agaatgctac gttaatcgct gcttcagtcg caccgtgacc aagcatggca cgaactggag
     4201 ccataaaaac atcgtaaatc ccttgtggat tttgagcaac cttctcatag gtaccagcca
     4261 ccaaacgtcc ggcttcatca acctgatact gaccagctgg aataacccat gtcatcactg
     4321 ccataaaggc aataatgagc atcaatattg tataagacga aggcaattta aagccttttt
     4381 tcactttttc gctcatcgca aaaacctcct ataatatttt aaaagtttat cccctattta
     4441 agggaaattg tatccgtaat aaccgtatca aatatccacc tatcttcaag agatgtctat
     4501 ctccttcagt ctattatctg aaatcattgc ttatagatag atacttgata ggaaatggat
     4561 gagaggggag ggagcttaga cgcccagacc ttcatgttta gattaaaggg aacccttgtg
     4621 ggactatcaa cacttccact cccctaactc catcaataaa taacccgtct agtttaaaaa
     4681 ctatatttgc atgcagagta catcaataaa tagaagcctc aacgatacat acagctgctt
     4741 agcctttgac aatgatagtg ccgctatcgg attcaatcaa agcgcctaag ttttctaatg
     4801 aggtaatgac tgcttttgat tgcggcttat tgtttacaaa agcaatagct gcttcaactt
     4861 tcggtagcat ggaacctggt gcgaattgat tttgtttgat atattcttcc aattcagcca
     4921 cagtaacggt ttctaatttt tcttggtctg gtttgttgta gttaacaaag acatagtcta
     4981 cacctgtcaa gacgataaac aagtctgcat caaccaattc tgccaaacat tgagaagcaa
     5041 agtccttatc gataactgct tccacaccgg tcagggtgcc atctgcttct tggacaactg
     5101 gaatacctcc gcctccagct gctacgacaa cttcacctgc attgagaagg gtacgaatgg
     5161 taccaatttc tttgattcct acaggttttg gcgaagcaac gactttacgc cagccacgac
     5221 cggcatcttc tttgaaggtt gcaccagtct tttctgcttc tgctttcgct tcttcttctg
     5281 tatagaatgg tccaatcggc ttggtcaaat tttcgaaggc gggatcattc ttatctacga
     5341 taacctgggt cacaacagat gctacatctt tttcaatgcc ttccttgagc aattcatttt
     5401 ccaaggcatt ttgcaaccag aaaccaatcg aaccttctgt catggctacc aaactgtcta
     5461 gtggaaaagc tgggttcttt tccgaatcag cagcaaggtt ttgaagaagt aagtttccaa
     5521 cctgaggacc attaccatgc gtgataataa ggtcatcccc attttttatc aattttacaa
     5581 ggtgcttggc tgtttgtacc aaggcctctt tttgtgcttt agccgatggg tcggacgata
     5641 aaatggcatt gccacccaag gctactacaa ttttacgatt tgccatatct cttgcttttt
     5701 cctcactttc gcgaaatagt aatcgcttac acatctatcc tcaaaataag ggggctgggt
     5761 gccagccccc ggaggttgcg atgcgttatt agatagttaa ttattacaca cgtggaacaa
     5821 atggatcccc taaagtagcc gccatcactg ctttaatagt gtgcatacgg ttttcagctt
     5881 ggtcgaagtg acgagcatac ttgctacgga atacttcgtc tgttacttcc atttcttcta
     5941 cgccgaattt ttcagcgaca tctttaccat aaacagtgtt tgtatcatgg aaggcaggca
     6001 agcagtgcaa gaagatgaga ttttcatttt ctgctttttt caccaattcc atattcactt
     6061 ggtatggctt caagagggca acgcgttcag caaatttatc ttcttcaccc attgataccc
     6121 aaacgtctgt atagagaaca tctgcgcctt tgactgcttc gtcagcattg tcagtaatga
     6181 gaacacgtgc tccactttct ttcgcaaaac cttcagccaa ggcaactact tcttcttctg
     6241 ggaagagttc ttttggagag aagatatgga cattgacacc aaggatagcg cctgttacca
     6301 aaagtgagtt agcaacattg ttacggccat caccacagta aaccagagtc aagccttcca
     6361 acttgccaaa gttttctttg acagtcaagt agtccgccaa catttgagtt gggtgccatg
     6421 catctgtcaa accattccat actggcacac cggagaattc tgccaattct tccaccattt
     6481 tttgactgaa accacggaat tcaataccgt cgaacatacg tcccaaaact ttagcagtat
     6541 cttctgtaga ttctttctta ccaagttgga tgtcatttgc accaagatat tctggatggg
     6601 caccaaggtc aatagatgct actgtgaagg cagcacgagt acgtgttgat gttttttcaa
     6661 acaagagagc aatattttta ccttccaaat aacggtgtgg aatattgcgt tttttcaaat
     6721 ctttcaaatg agctgagaaa tcaatcaacc attccaattc tgcgcgtgtg aaatctttct
     6781 ctgcaaggaa atgtctacct ttaaatacgt ttgtcattgt ctcttctcct aatgtttaaa
     6841 taaattttgt caatgtctgg caccattctc catcttcttg ataaacttca gaagttgctt
     6901 ggtagccaac tgactcataa aaagtctttg cagttaactc cgaatgtata ttcaactgat
     6961 gaaagcctgc ttcacgtgca tacgcttcca aagagctgat aatcattttt ccatagcctt
     7021 ttccacgaaa attcggtaga gtggctattc gggttagacg agcttgacca ggaccgacag
     7081 gaagaaaacg tcccgttgaa acaggctctt caccaataaa gagattggcg taaactcttt
     7141 cttccgtatc attctggtca aattcatctt cctttgcaat accacgctca aggacaaata
     7201 ccttatagcg gacatagata gaggctgcgc gttgccattc ttccgaaccg attgttgttc
     7261 gttctatact tggcattaga tgtcttcacg ttcaaacggc attgacatac aacgaggtcc
     7321 accacgaccg cgaaccaatt cgcttccgcc aatcttaatc aaacgaagcc ctttggactc
     7381 gaggatagca ttggtaatcg tattgcgttt gtaaacaact accacacctg gtgcaatagt
     7441 caatgtattt gaaccatcat tccattgctc acgaccagca gcaaccatat caccgccacc
     7501 acaacgaatc aattctactt tttcaagacc aaggttcgca gccaaaagtt ctgccaaatc
     7561 accatgttct tcttcaatat gaagcgtgtc attttcatag gttacagaga agacacgaag
     7621 gtcaccttcg atttctgggt ggatagtaaa cttatcatag tcaaccatgg taaatactgt
     7681 atccaaatgc atgaatttac ggttgttagc aaattcgaat gccaatactt tcttgaagcc
     7741 aacatgccgt tcaaagatat ttaccaatag tttttcaatt gatgccgcat cggtacgttg
     7801 tgaaatacct actgccaaaa catcttttga aagaaccaat tcgtcaccac cctctatacg
     7861 agtagtttct tcacgattgt aaaccaatgg aacttttcca ccatattctg ggtgatgagt
     7921 gaagatatat ttaccataca aggtttcacg gttccgtgtt tctgaataca tgtggttaag
     7981 agaaacagca ttaccaatcg tagcaaatgg atcccgtgtg aagtaaaggt ttggcattgg
     8041 gtctattgca aatgggtaag aggattcaac caaatctgtc aaaccttttt cttcagaagg
     8101 gatttctggc aattctgcct tttgaacacc agccatcgtt ttctcaacca gttcttgatt
     8161 atcttcaata gacatcaaca gcttacgaat tgccttttta gttgcccggc cacgaatgtt
     8221 agcttcttca agatattcat caataaattg ttcacgaatt tcaggtgtaa caagtgactc
     8281 agcagctaat ttttccaagt agagcacttc tacaccttca tcacgaagag cttgagcaaa
     8341 ggcatcatgc tctttctgtg catcttctaa gaatgggata tcatcgaaga gtaggcgttc
     8401 tagatagtca ggcataaggt tttcaatttc cttacctggt ctgtgtaaca taactttttt
     8461 cagcttacca atttctgaaa aaacatgaat tggatggttt gacatgatct gtcctccttg
     8521 tagatttata catgatggga aacgaggagg accgtacgct ttcctgcctc tcatttcctc
     8581 tttacatcca tagtttaaca gagcaaaaaa acaaaaactt gaaaacgctc acatttattt
     8641 tgagaaattc ttcacatttt taaccttatt tataaataat tatcgattat atcatct
//
LOCUS       DBNFCK010000078         8608 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.078, whole genome shotgun sequence.
ACCESSION   DBNFCK010000078 DBNFCK010000000
VERSION     DBNFCK010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8608
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.078"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(105..710)
                     /gene="sodA"
                     /locus_tag="AC1PIW_001385"
     CDS             complement(105..710)
                     /gene="sodA"
                     /locus_tag="AC1PIW_001385"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase SodA"
                     /protein_id="HIP6606315.1"
                     /translation="MTIILPDLPYAYDALEPHIDAETMTLHHDKHHATYVANANAALE
                     KHPEIGEDLVALLSDVEQIPSDIRQALINNGGGHLNHALFWELLSPEKTEISAELAAD
                     IDATFGSFDAFKEAFTAAATTRFGSGWAFLVVNKEGKLEVISTANQDTPIMQGLKPIL
                     ALDVWEHAYYLNYRNVRPNYIKAFFEVINWDKVNELYKAAK"
     gene            complement(823..1854)
                     /gene="holA"
                     /locus_tag="AC1PIW_001386"
     CDS             complement(823..1854)
                     /gene="holA"
                     /locus_tag="AC1PIW_001386"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIP6606316.1"
                     /translation="MTVIEQIEKLKKEKLELLTVLCGEDIGQYQIAKDLLLRKLHFDA
                     ADLGFAYFDMSEADYHQVDLDLVSLPFFSDEKVVILDYFADLTTDKKRHLTDDELKQF
                     EAYLENPVETTRLVILAPGKLDSKRRLVKLLKRDGLVLEANPLKEMDLKNHFQKEIMR
                     LGLQMGGEVFQYLLEKSNFDFAEISKNLAFLQSYKGKETIEISDIDAAIPKTLQDNIF
                     DLTQLILQSKVDDARNLVCDLRLQGEEEVKLIAIMLTQFRTYLQVQILLEQGRGEQQI
                     VAELSTIMGRKVNPYQVKYALRDSRHLSIGFLKKVVKLLIDTDFQIKTGQFDKDYLFD
                     LLLLKIATT"
     gene            complement(2005..2595)
                     /locus_tag="AC1PIW_001387"
     CDS             complement(2005..2595)
                     /locus_tag="AC1PIW_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606317.1"
                     /translation="MKLRKVSFILVTFVIGLLSLSSVKAETSIFVPVGQQNVPGVKVA
                     PFRTGSDSIHVHVGSFGYVATYINGERLKVEPVKHELKCPSYTTENCQTREWYTSGER
                     ADGSGDYVVKLNKKLEEGDVVTLKFADDGNLYFGQLVYKSEKKRVEQNQKEQEDEYAD
                     ALFKRSIEEEENKTWRDRIKDTFQDTWWNFKGWWNS"
     gene            complement(2703..3458)
                     /locus_tag="AC1PIW_001388"
     CDS             complement(2703..3458)
                     /locus_tag="AC1PIW_001388"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HIP6606318.1"
                     /translation="MIKVEEILSTLSPNQKVNYDKVFQKMASTWQEAGLRPNILMHVC
                     CAPCSTYTLEYLTQYADVTIYFANSNIHPKAEYQRRAAVTAQFVKEFNQKTGQSVGYL
                     EADYSPQEFFKKTQHLANEPEGGDRCKVCYDYRLDKTAEKAVELGFDYFGSALTISPH
                     KNSQTINAVGIEVQKLYDTQYLPSDFKKNGGYQRSVQMCEEYDIYRQCYCGCVYGANA
                     QGVNLIQVKKEALEFLKTHRDYSHIPFKVLDKN"
     gene            complement(3888..4598)
                     /locus_tag="AC1PIW_001389"
     CDS             complement(3888..4598)
                     /locus_tag="AC1PIW_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6606319.1"
                     /translation="MAMLEVKNLSVNYGVIEAVKDVSFEVNEGEVVTLIGANGAGKTS
                     ILRTISGLVRPSAGTISFLGNEIQKVPARKIVADGLSQVPEGRHVFAGLTVMENLEMG
                     AFLHNNREENQANLRKIFARFPRLEERKNQDAATLSGGEQQMLAMGRALMSQPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAIADRGYVLETGKVVLSG
                     TGKELLASEEVKKAYLGG"
     gene            complement(4598..5362)
                     /locus_tag="AC1PIW_001390"
     CDS             complement(4598..5362)
                     /locus_tag="AC1PIW_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6606320.1"
                     /translation="MALLEVKDLTKNFGGLTAVGDVTMELHEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTISLAGTILNGKAPSKIASLGLGRTFQNIRLFKNMTVLENVLIG
                     LGNHGKSEVFASFFRLPAFYKNEEALKTKAIELLKIFDLDGDADTLAKNLPYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTQLIRKIKEEFGITIILIEHDMSLVMEV
                     TERIYVLEYGRLIAHGTPEEIRNNKRVIEAYLGGEA"
     gene            complement(5362..6309)
                     /locus_tag="AC1PIW_001391"
     CDS             complement(5362..6309)
                     /locus_tag="AC1PIW_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIP6606321.1"
                     /translation="MKQNLKVNAIWLAILLAGFGLIQGLVSAGILNFYYIQIVQQIGI
                     QIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAVGILGKMMPTYGGFAIALIVGMLISG
                     AVALLVGIPTLRLKGDYLAIATLGVAEIIRILIINGGSVTNGAAGIMSIPLFTSWPLV
                     YIFVIITTLVTVNFLRSPMGRATISVREDEIAAESVGVNPTFVKVSAFVFGAMTAAIA
                     GGLHAGYVGTIVPKDFAFMTSVNVLIIIVLGGLGSITGTFVAAIVLGILNVFLKSYSD
                     ISMIIYSLALILLMIFRPGGLLGTKEFSVAALLKKKEVK"
     gene            complement(6312..7199)
                     /locus_tag="AC1PIW_001392"
     CDS             complement(6312..7199)
                     /locus_tag="AC1PIW_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIP6606322.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDLYMMGA
                     FMGYFLLNNLTFIADGGTRFFVALILAMVGTAILGVVIEFLAYRPLRNSTRIAALITA
                     IGVSFFLEYTMIKFFTADVKAFPQAIEAVTFNLGPVSVTNIQLIILGVSLALMVALQL
                     IVKRTKMGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLLGLYYNQIE
                     PLMGMTPGLKAFVAAVLGGIGIIPGAALGGFVIGIIETFTYVIEFDTFRDAIVYTVLI
                     IILLVRPSGILGKNVKEKV"
     gene            complement(7415..8584)
                     /locus_tag="AC1PIW_001393"
     CDS             complement(7415..8584)
                     /locus_tag="AC1PIW_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIP6606323.1"
                     /translation="MKTRKFAVALATFASAALLAACGNVSTTNTSAAGSSVGDTFKIG
                     YNLELSGAVSSYGQTEEKGANLAVKEINAAGGIDGKKIEVITKDNKSETAEAATVATS
                     LASEGANVVIGPATSGASAASIPALTSAGVPMITPSGTQTTLVVNDKGEVQEYFFRTT
                     FTDGYQGQIMAKYATENLSAKKVVLYFDNSSDYGKGVAEAFKKAYTGEIVSEITFASG
                     DKDFQAALTKLKDKEFDAIIMPGYYNETGTIVKQARGLGIDKPILGSDGFDSPQFTEL
                     ATASAASNVYYLSGFVTSASEKAKAFYDAYVKEYNEEPSMFSALAYDSVYMAAEAAKG
                     AADSAAVKANLAALKDFKGVTGTMSVDKEHNVIKSVYVVSLTNGEESSVDTISID"
BASE COUNT         2624 a         1943 c         1522 g         2519 t
ORIGIN      
        1 catttttatc tataatacga aaattcccct tcttccctta cacaattctc tccaaaatac
       61 tcctcacaac taaaaaatct cttccaaacg gaagagaact gttcttattt agctgcttta
      121 taaagttcgt taaccttgtc ccagttgatt acttcaaaga aggctttgat gtagtttgga
      181 cggacattgc ggtagttaag gtagtaagca tgttcccaaa cgtccaatgc caagattggt
      241 ttcaaacctt gcatgattgg tgtgtcttgg ttagcagttg agataacttc caacttgcct
      301 tctttattaa caaccaagaa agcccaacct gaaccgaaac gagttgttgc tgctgcagtg
      361 aaggcttctt tgaaagcatc aaatgaacca aaggttgcat caatatctgc tgccaattct
      421 gctgaaattt ctgttttttc aggtgaaagc aattcccaga aaagagcatg gttcaaatga
      481 ccaccaccgt tgttgatcaa agcttggcgg atgtctgaag gaatttgctc cacatctgac
      541 aaaagagcta ccaagtcttc gccaatttca ggatgttttt caagtgctgc attagcattt
      601 gcaacataag tcgcatggtg cttatcgtgg tgaagggtca ttgtttctgc atcaatatgt
      661 ggttccaagg catcgtatgc gtatggaagg tctggtaaaa taattgtcat tttcgttttc
      721 tcctatacta agtgatagtt ctattctact ccaatgtgag agccttttca atttatttgt
      781 ctgaaaccac ggttgaccct caatctaaga atactctttt tactatgtag tcgcgatttt
      841 taataatagc aaatcaaata aataatcctt atcaaattgg ccggttttaa tttgaaaatc
      901 tgtatcgatt aggagcttga cgactttttt caaaaatcca atggacagat ggcgtgaatc
      961 acgcaaagca tactttactt ggtatggatt caccttacgc cccataatag ttgataactc
     1021 agctactatc tgctgttccc ctcggccttg ctctagtagt atttgcactt gtagataggt
     1081 tcgaaattgg gttaacatga tggcaatcaa cttaacctct tcctcccctt gcaaccgcaa
     1141 gtcacaaact aagttgcgag catcgtctac cttggattgt agaattaact gtgttaaatc
     1201 aaagatatta tcttgtagtg tttttgggat agccgcatca atatctgaaa tttcaatcgt
     1261 ctcttttccc ttataagatt gaagaaaggc taggttctta ctgatttctg caaaatcaaa
     1321 attggacttt tctaaaagat attgaaaaac ctctcctccc atctgcaatc caagacgcat
     1381 tatctccttt tggaaatgat ttttcaaatc catctccttc agaggattgg cttctaatac
     1441 tagtccatct cgtttgagga gtttgaccag acggcgctta ctgtccaact tacccggtgc
     1501 tagaatgacc aaacgtgttg tttcgacagg attttccaaa taggcctcaa actgtttgag
     1561 ttcatcatct gtcaaatgcc gttttttatc tgtcgttaaa tctgcaaaat aatcaagaat
     1621 cactactttt tcatctgaaa agaagggaag agaaactagg tccaagtcta cctgatgata
     1681 atcagcctcc gacatatcaa aataggcaaa acctaaatct gccgcatcaa aatgaagctt
     1741 ccgcagaagt aagtctttcg ctatttggta ttgtccgatg tcttcaccgc ataaaacggt
     1801 cagaagctct aatttctctt tttttaattt ttcaatctgt tcaattactg tcataaaaac
     1861 ctcaaaattc ttttctcatt gtaccacaaa aagagcctag acataaagtc tggactcttt
     1921 cattgaaatt tatatcagga aggtaggaaa aaattaattg ataatcactc caaatgacct
     1981 atttcccaaa tagcagttcc tttgttagct attccaccat cctttgaaat tccaccaagt
     2041 atcttgaaaa gtatccttta tccgatctct ccaagtctta ttttcttctt cctcaataga
     2101 acgtttgaac aaggcatctg cgtactcatc ctcttgttcc ttttgattct gttcaactcg
     2161 tttcttttcg cttttatata ctaattgacc aaaatacaaa ttcccatcgt ctgcaaattt
     2221 taatgtaaca acatcacctt cttcgagttt cttattcaat tttacaacat aatctccact
     2281 gccgtccgca cgttccccac ttgtatacca ctctctagtt tggcaattct ctgtagtata
     2341 actcggacac ttgagttcat gctttacagg ctcgaccttc aatcgttctc catttatata
     2401 tgtagcgaca taaccaaatg agccaacatg gacatgaata gagtctgaac cagttctaaa
     2461 aggagctact tttacccccg gaacattctg ttgacctact ggtacaaata ttgaagtctc
     2521 cgcctttaca cttgataacg atagtaaacc gatgacaaag gtaactaaaa taaatgatac
     2581 ttttctcaat ttcattttgc ttttctccta gtagtacaat acattaaccg gaattagtat
     2641 ataccaattt catagagaaa gcaagaaaaa acgctcctta agtaaaaact aaactcaaaa
     2701 actcaattct tatccagcac cttgaaagga atgtgggaat aatctctgtg cgtttttaaa
     2761 aattccaagg cttctttttt tacctgtatg agattaacac cttgggcatt ggctccatag
     2821 acacagccac aatagcactg gcgataaatg tcatactctt cacacatttg aactgaacgc
     2881 tgatagcctc catttttctt aaagtcactc ggaagatact gggtatcgta taatttctgc
     2941 acctcaatcc caacagcatt gatggtctgc gaattcttat gtggactgat tgtcaaagcc
     3001 gaaccaaaat agtcaaaacc aagctcaact gccttctccg cagtcttatc caaccgataa
     3061 tcatagcaaa ccttacaccg atctcctccc tctggctcat tggcgagatg ctgagttttc
     3121 ttgaaaaatt cctgagggct atagtcagct tctaggtagc ctactgactg tccagttttc
     3181 tgattaaatt ctttgacaaa ctgggccgtc acggctgccc gccgctggta ctccgccttt
     3241 ggatgaatat tggaattcgc aaaatagatg gttacatctg catattgagt caagtactcc
     3301 aatgtatagg tcgagcatgg ggcacaacag acatgcatga gaatgttcgg tcgaagcccc
     3361 gcttcctgcc acgtgcttgc cattttttga aacactttat catagttaac cttttgattc
     3421 ggtgatagag tcgataaaat ctcttcaact ttaatcatac aaacctccaa atatacattc
     3481 attatactat atttgaagcc tactgcatag atagtaataa cgagggagag cgcttgataa
     3541 ggatccctat ttccaactta aaaaggtccc tcggaccttg atataaagaa aaacgcctgc
     3601 cgacgtttct catctccagc ttaaaacgtg gggtaaccca cttgtaaaga aaaccgctta
     3661 ccagcggttc tcatttccaa cctaaaacaa cccgtcaggt tggggacaaa ccagcaacat
     3721 gcattgctgg ttttcctatc tcccgattaa aacagcctat ccgaatataa aaaaactgcc
     3781 tgccggcagt tctcattttc aacttaaaac gtggggtaac ccacttataa agaaaaccgc
     3841 ttaccagcgg ttctcatttc caacctaaaa aggtcccccg gaccttttta gccacccaga
     3901 tatgcttttt tgacttcttc tgaggcgagg agttcttttc ctgttcctga gaggacaact
     3961 tttcctgttt ctagaacata gccacgatcg gcaatagcga gagctttgtt ggcattttgc
     4021 tcaatcaaga ggacagttgt tccttgtttt tgaatatctt ggatgatatc aaaaatttct
     4081 tggataaaga tcggagccaa gcccattgat ggttcatcaa ggagcaagag ctttggttgg
     4141 ctcattaggg cacgtcccat ggccaacatc tgttgctccc cacctgaaag ggtagctgca
     4201 tcctggtttt tacgttcttc caaacgtgga aaacgagcaa agatttttct caagtttgct
     4261 tggttttctt cacgattgtt gtgaaggaag gcacccattt ccaagttttc cataacagtc
     4321 aagcctgcaa aaacatgacg accttctgga acttgtgata aaccgtccgc cacgatttta
     4381 cgagcaggaa ctttttgaat ctcatttcca aggaaagaaa tcgttccagc tgatggacga
     4441 acaagccctg aaatagtacg aaggatagag gttttaccag caccattagc gccgataagg
     4501 gttacaactt caccttcatt aacctcaaag ctaacatctt taacagcttc gataacaccg
     4561 tagttaacgg aaagattttt tacttctaac attgccatta agcttcacct ccaaggtagg
     4621 cttcaattac acgcttgtta ttacgaattt cttctggtgt accatgggca atcaaacgac
     4681 cgtactccaa tacgtatatc cgctccgtca cttccataac caagctcata tcgtgttcaa
     4741 tcaagatgat agtaatgcca aattcttctt tgattttacg gatgagttgg gtcaattctg
     4801 ccgtttcttg tgggttcata ccagccgctg gctcatccaa gaaaagaatt ttcggttcag
     4861 tcgcaagggc acgaacgatt tccaaacgtc gttgttgacc gtaaggaagg tttttagcta
     4921 atgtatctgc atctccatcc aaatcgaaga ttttcaaaag ttcaatcgct tttgttttca
     4981 acgcttcttc attcttatag aatgctggaa gacggaagaa acttgcaaat acttctgatt
     5041 taccatggtt gccaagacca atcaaaacat tttccaaaac ggtcatattt ttgaacaaac
     5101 gaatgttctg gaaagtacga cctagaccaa gcgaagcaat ttttgatggt gctttaccgt
     5161 tcaaaatagt ccctgccaga gaaattgtcc cctcgcttgg ctcataaaca cctgttaaga
     5221 ggttgaaaag agtcgtttta ccagcaccgt ttggaccgat aagaccaacc aattcccctt
     5281 catgaagttc catggtcacg tcaccaacgg cagtcaggcc accgaagttc ttggttaaat
     5341 cttttacttc aagtaatgcc attacttaac ctccttcttc ttgagtagag ccgctacgct
     5401 gaattcctta gttcccaaaa gacctccagg acggaaaatc atcaagagaa taagagctaa
     5461 tgaatagata atcattgaaa tatcagagta actcttgagg aatacgttca aaataccaag
     5521 tacaatggct gctacaaagg tacctgtgat agagcccaaa ccacccaata caatgataat
     5581 caaaacgtta acagaggtca taaaggcaaa gtctttcgga acaattgtcc caacgtatcc
     5641 tgcatggaga ccacctgcaa tggctgccgt catggcacca aatacaaagg ctgaaacctt
     5701 aacaaatgtt ggattgacac ctacagattc tgccgcaatt tcatcctcac gaacagaaat
     5761 tgtagcacgt cccatcggac tgcgcaaaaa gttaactgtc accaaggttg tgatgattac
     5821 aaaaatataa actaaaggcc agcttgtaaa gagtggtata gacataatac ctgctgcacc
     5881 attcgttaca ctaccaccat tgataattaa aatacgaata atctctgcaa caccaagagt
     5941 cgcaattgca aggtagtcac ctttcaaacg aagggttgga ataccgacta aaagagctac
     6001 tgcacctgag attaacatcc caacaatcaa ggcgatagca aatccaccat aagtcggcat
     6061 cattttccca agaataccta ctgcataagc accaatagcc ataaaaccag catgaccaag
     6121 tgaaaattga cctgagaaac cgacaatcaa gtttaaacca actgccaaga taatttgaat
     6181 accgatttgc tgaacaatct ggatgtagta aaaattcaga atccctgctg atacaagacc
     6241 ttgaataagt ccaaaaccag ccaataagat agccaaccaa atcgcattaa cttttaaatt
     6301 ttgcttcatg gttacacctt ctctttcaca tttttaccaa gaataccact tggacgcacc
     6361 aagaggatga tgatcaaaac agtatatacg atcgcatcgc ggaaagtatc gaattcaatc
     6421 acataggtga atgtttcgat gataccgata acgaagccac caagcgctgc acctggaata
     6481 ataccgatac cgcccaatac tgccgcaaca aaggctttca gacctggcgt catccccatc
     6541 aaaggctcaa tctggttgta gtagagacct aggagaacac ccgcagcacc cgcaagagct
     6601 gacccaagag caaaagtaaa gctgattgta cggtttacat tgatccccat aagctgagct
     6661 gcatcgctat ctacagagac cgcacgcatg gctttcccca tttttgtacg tttgacaatt
     6721 aattgaagtg caaccatcaa cgcaagcgat acaccaagaa taatcaactg gatattcgta
     6781 acagaaactg gaccaaggtt aaaagtcaca gcttcgattg cttgtggaaa ggcttttacg
     6841 tctgctgtaa agaatttaat catagtgtat tctaagaaga aggatacacc gatagccgta
     6901 atcaaagctg ctatacgcgt tgaattacgc aaaggacgat aggctaaaaa ctctataact
     6961 acaccgagaa tagccgtccc taccatcgct agaatgagag caacaaagaa acgcgttcca
     7021 ccatctgcaa taaaagttaa attatttaat aggaagtacc ccataaaagc acccatcata
     7081 tacaaatcac catgggcgaa gttaatgagt ttgataattc cgtacaccat ggtataacca
     7141 agggccaaaa gggcataaac agaaccaaga atcaaaccat tgacaagttg ttgaagcata
     7201 ggttcaccaa tctttctaaa tatttttcga gctcaatagc attgttacat tatacaaaat
     7261 tttctgaaaa taagcaagtt agacagagag tttaatcatt aaaattacag aaaatttaac
     7321 caacccaaat ttaaccaaat tttatatccg catgtatact attctataca ttctgacaag
     7381 tgaaaaagag ggaattccct cttttcatcc actattagtc aatagagata gtatctacag
     7441 atgattcttc tccgtttgtt aaactaacaa cataaactga tttaatgaca ttgtgctctt
     7501 tgtcaacaga cattgtacca gtcacacctt taaagtcttt cagagctgca agattagcct
     7561 tcactgctgc tgaatcagct gcaccttttg ctgcttctgc tgccatgtaa accgagtcgt
     7621 aagctaaagc tgagaacatt gatggctctt cattgtattc tttcacatag gcatcataga
     7681 atgcttttgc tttttcgctt gctgatgtta caaatccaga aaggtagtaa acatttgatg
     7741 ctgctgatgc agttgccaat tcagtaaatt gtggtgagtc aaaaccatct gaaccaagaa
     7801 ttggcttgtc aatacccaaa ccgcgtgctt gcttcacaat tgtacctgtt tcattgtagt
     7861 aaccaggcat gatgatcgca tcaaattcct tatctttcaa ttttgtcaaa gcagcttgga
     7921 aatctttatc gccagaagca aatgtgattt ctgaaacgat ttctcctgtg tatgctttct
     7981 tgaacgcctc tgctacaccc ttaccataat cagaagagtt gtcaaagtaa agaacaactt
     8041 tcttagctga caagttctca gtagcgtatt tagccataat ttgaccttga tacccatctg
     8101 taaaggttgt acggaagaag tattcttgga cctcaccttt atcgttcaca accaaggttg
     8161 tttgagttcc tgaaggagta atcataggaa caccagcaga tgtcaatgct ggaatagagg
     8221 ctgctgaagc tcctgatgta gcagggccaa tcacaacgtt tgccccttca cttgccaagc
     8281 ttgttgcaac agtagctgcc tctgctgttt cagacttgtt gtcttttgtg atcacttcga
     8341 tttttttgcc gtcaatacca cctgcagcat tgatttcttt cacggcaagg ttggcacctt
     8401 tttcctctgt ttgaccataa gatgaaacag cacctgacaa ctccaagttg tagccgattt
     8461 taaatgtatc acctacagaa gaacctgcgg ctgaagtatt ggttgtcgaa acattaccac
     8521 atgcagcgag tagagctgca gaggcaaatg tagcaagcgc tactgcaaat tttcttgttt
     8581 tcatgaaaac tccttaaatc tttcttaa
//
LOCUS       DBNFCK010000079         8602 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.079, whole genome shotgun sequence.
ACCESSION   DBNFCK010000079 DBNFCK010000000
VERSION     DBNFCK010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8602
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.079"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            11..1027
                     /locus_tag="AC1PIW_001394"
     CDS             11..1027
                     /locus_tag="AC1PIW_001394"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIP6606324.1"
                     /translation="MTKQTIAILGPGSWGTALGQVLNDNGHTVRIWGNVPEQIDEINK
                     EHTNKRYFKDVILDENIKGYKDLSEALDGVDAVLFVVPTKVTRLVAKQVAQALKHKVV
                     VMHASKGLEPDSHKRLSEVLEEEIPAELRSEIVVVSGPSHAEETIVRDLTLISAASKD
                     LETASYVQNLFSNHYFRLYTNNDVIGVETAGALKNIIAVGAGALHGLGYGDNAKAAII
                     ARGLTEITRLGVAMGANPLTYSGLSGVGDLIVTGTSVHSRNWRAGDQLGRGEKLEDVE
                     RNMGMVIEGISTTKAAYELAQELGVYMPITQAIYKVIYQGAGIEDAIKEIMTGEFRHE
                     NEWH"
     gene            1072..1971
                     /gene="galU"
                     /locus_tag="AC1PIW_001395"
     CDS             1072..1971
                     /gene="galU"
                     /locus_tag="AC1PIW_001395"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-alpha-D-glucose metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HIP6606325.1"
                     /translation="MKKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEE
                     ALRSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKNDLLKLVDETTGIRLHFIR
                     QSHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDTKAVPLTKQLMNDYEKTHASTI
                     AVMPVPHEEVSAYGVIAPQGEGVNGLYSVETFVEKPKPEDAPSDLAIIGRYLLTPEIF
                     EILENQKPGAGNEIQLTDAIDTLNKTQRVFAREFKGNRYDVGDKFGFMKTSIDYALQH
                     PQVKDDLKQYLIDLGKKLDTKKN"
     gene            complement(1989..2666)
                     /locus_tag="AC1PIW_001396"
     CDS             complement(1989..2666)
                     /locus_tag="AC1PIW_001396"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIP6606326.1"
                     /translation="MKNFFDKRYPVTNGLLAVTALVFLLIQVFRFGQTTTAYTIFEFG
                     GMYGQVVRYDPTQLWRLISPIFVHIGWEHFIFNSITLLGLGYQLEGLFGSRRFFLLYL
                     LSGIMGNLFVLFFTPDVVGAGASTSLFGLFAAMALLRKFSRSPYLQVLGQRYMVLLGL
                     NLVLGLFNPTISMAGHIGGAVGGCLVVMFLPPLVEKDLFTGKQIFFSFIGYLGLIALL
                     LGIFYLT"
     gene            complement(2650..3192)
                     /locus_tag="AC1PIW_001397"
     CDS             complement(2650..3192)
                     /locus_tag="AC1PIW_001397"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HIP6606327.1"
                     /translation="MDKKSIRQEVLQSLKDLTSSQRARWSQQLTERLLASDTYQGAKS
                     LGTYLSMPHEFDTSYLIEQAQKDGKQIFIPKTYSQGRMDFVEYNPDDLVKSRFGVWEP
                     GIYSQPVDKSVVKLLHVPGLAWNQAGFRVGYGGGFYDRYLADYQGETVSTLADFQVYD
                     FEPDVFDIPVKELLIFEELF"
     gene            complement(3233..4360)
                     /locus_tag="AC1PIW_001398"
     CDS             complement(3233..4360)
                     /locus_tag="AC1PIW_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HIP6606328.1"
                     /translation="MLDLIATRRALHQIPELGMEEFKTHAFLMETIEGLLQDCSFAQV
                     RTWKTGILVYLTGSAPEKTIGWRADIDGLPIVEETGLDFKSLHPDRMHACGHDFHMTI
                     ALGLLEKMAEQQPRNNLLFLFQPAEENLAGGMLMYEAGAFGDWLPDEFYGLHVRPDLK
                     VGQMATNRATLFAGTCEVKIRFTGKGGHAAFPYTANDALVAASYFVTQVQSVVSRNVD
                     PIEGAVVTFGSMHAGTTNNVIAETAFLHGTIRALTQNMSLLVQKRVREVAEGIALSFG
                     VDLEIELNPSGYLPVENNPQLADELMTYFDGIDGVEMIDCPPAMTGEDFGYLLNKVPG
                     VMFWLGVDTPYPLHNPRLSPKEEVLPFAVDKLSDFLKMKAN"
     gene            complement(4370..5068)
                     /gene="dapD"
                     /locus_tag="AC1PIW_001399"
     CDS             complement(4370..5068)
                     /gene="dapD"
                     /locus_tag="AC1PIW_001399"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HIP6606329.1"
                     /translation="MTAQKMTAQEIIAFIGNAVKKTTVKVTFEGELAGAVPAEVTKLG
                     NVLFGDWKDVEPLLANLTENVDYVVEQDGRNSAVPLLDKRNINARIEPGAIIRDQVTI
                     GDNAVIMMGAVINIGAEIGPGTMIDMGAILGGRATVGKNSHIGAGAVLAGVIEPASAE
                     PVRVGDNVLVGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEEALRTL"
     gene            complement(5343..5810)
                     /locus_tag="AC1PIW_001400"
     CDS             complement(5343..5810)
                     /locus_tag="AC1PIW_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606330.1"
                     /translation="MSKDDYSSTRKQEFEQILNSVDISDEVVESSSKTTETPTSSEST
                     ATVSEFNPTDSSDATIESIKTYSDYMKMYEFIVNEYITNYENMVSQYGLGDATMYQSV
                     RDTVSQSVEQQKKQYGPLGNAAIVGRAEIITFLKEYRDGLQQQIEQMSAALGG"
     gene            complement(5797..6333)
                     /locus_tag="AC1PIW_001401"
     CDS             complement(5797..6333)
                     /locus_tag="AC1PIW_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606331.1"
                     /translation="MAKKIRDEHGNVYVQKKPFYKRIWFIVLVGLFAIGLIKNLTSSS
                     GGQSSTEQVSTSSIRYKTYQLSEVAISINESWKSKEGDTSDTLYFYPDDSSLAMAYWL
                     PLEESVKDATVRSQYLKGMEQSGKVIPANESSAKIDGMDSFLYDATGTIDGEAYKGQL
                     FLVDTPTGIFKCNLYVKG"
     gene            complement(6441..7181)
                     /locus_tag="AC1PIW_001402"
     CDS             complement(6441..7181)
                     /locus_tag="AC1PIW_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIP6606332.1"
                     /translation="MKKEFFVIGDVHGKFELLLDILKKWNEKRQQLIFLGDLIDRGEN
                     SKACLELVWSLVREKGAICLTGNHERMFLAWLRDPIDRYDHYRRNGGDTTINSLLGRP
                     LDAPVDGLVDANAVLEQYEDLIDFVKSCPYHLETEDYIFVHAGVDLTLPDWRETSNHD
                     KVWLRTPFHEAENTTGKMIVFGHTPVYNLYQTKLRISQIWTSPDGKMGIDGGAVYGGV
                     LHGIVLDGTGLVQDYIVGAVNPRKEIED"
     gene            complement(7247..8596)
                     /locus_tag="AC1PIW_001403"
     CDS             complement(7247..8596)
                     /locus_tag="AC1PIW_001403"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HIP6606333.1"
                     /translation="MTHITFDYSKVLGQFVGAQEVEYMQPQVTLADQMLRQGTGPGSD
                     FIGWLDLPENYDKEEFARIKEAAAKIQNESEVLVVIGIGGSYLGAKAAIDFLSNAFVN
                     LQTREERKAPQILYAGNSISSSYLADLVDYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDKAKGAVKVEADANGWETFVVPDDVGGRFSVLTAVGLL
                     PIAAAGADIDALMEGANAARTTFTSDKIAENQAYQYAAVRNILYRKGYVTEILANYEP
                     SLQYFSEWWKQLAGESEGKDQKGINPTSANFSTDLHSLGQFIQEGNRNIFETVVRVDK
                     PRKNILIPEMAEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGGVPNMFVTLPQQDEF
                     TLGYTFYFFELAIAISGYLNAINPFDQPGVEAYKKNMFALLGKPGFEELGAELNARL"
BASE COUNT         2482 a         2053 c         1736 g         2331 t
ORIGIN      
        1 aggatttttt atgaccaaac aaaccattgc catcttggga cccggttcat ggggcaccgc
       61 cctaggacaa gttctcaacg acaacggtca tactgtccgt atttggggaa atgtccctga
      121 acaaatcgac gaaatcaata aagaacatac aaataagcgc tatttcaagg atgttatcct
      181 agatgaaaac atcaaaggat ataaggattt gagtgaagcc ttggacggtg ttgacgcggt
      241 tcttttcgtt gtgccaacca aggtaacacg cctagttgcc aagcaagtcg cacaagctct
      301 caaacacaag gtagttgtca tgcacgcttc aaaaggctta gagcctgata gtcacaagcg
      361 cttgtctgag gtactggagg aagaaattcc tgctgaactc cgttctgaaa tcgtcgtggt
      421 ttcagggcca agtcatgcag aagagactat cgtccgtgat ttgaccttga tttctgcagc
      481 ttcaaaagac ttggaaacag ccagctacgt ccaaaatctt ttcagcaacc actacttccg
      541 actttacaca aataatgatg tcatcggcgt agaaacagca ggtgccctta aaaatattat
      601 tgctgtcggt gccggcgctt tacacggact tggttatggt gataatgcca aggcagctat
      661 tatcgctcgc ggtttgacag aaatcacacg tctaggcgtt gctatgggcg caaatcctct
      721 gacttacagc ggtctttctg gtgttggtga tttaattgtt accggtacat ctgtccactc
      781 ccgcaactgg agagctggag atcagctcgg tcgcggtgaa aaactcgaag atgtagaacg
      841 caatatgggg atggtgattg aaggtatttc aacaactaaa gcagcctatg aattggcaca
      901 ggaattgggc gtttacatgc ctattactca agccatctac aaggtcatct accaaggtgc
      961 aggtattgaa gatgccatca aagaaatcat gacgggtgaa ttccgacatg aaaatgaatg
     1021 gcattgattt ttattaaaca gaaaaaataa aattaaaagg attacatact tatgaaaaaa
     1081 gttagaaaag ccgttatccc tgcagcaggt ttgggaacac gtttcttgcc tgccactaag
     1141 gctcttgcca aagaaatgtt gccgattgtt gataaaccaa ctattcaatt tatcgttgaa
     1201 gaagctctac gatctggtat cgaagatatt ttggtggtca ctggtaaatc aaaacgttct
     1261 atcgaagacc actttgattc aaactttgaa ttggaataca acctcaaaga aaaaggcaaa
     1321 aatgatttgt tgaaacttgt tgatgagaca acaggcattc gactacactt cattcgtcaa
     1381 agccatccac gtggacttgg tgatgccgtt cttcaagcta aagcatttgt cgggaacgag
     1441 ccatttgttg tcatgctggg cgatgacctc atggacatca cagatacaaa agctgtccca
     1501 ttgactaagc aactcatgaa cgactatgaa aaaacacatg cttcaaccat tgctgttatg
     1561 cccgttcctc atgaagaagt ttctgcatac ggtgttatcg ctccacaagg tgaaggtgtc
     1621 aacggtctct atagtgtgga aacctttgtg gaaaaaccaa aaccagaaga cgctccttct
     1681 gaccttgcta ttatcggacg ctacttactc actcctgaaa tctttgagat tttagaaaac
     1741 caaaaaccag gtgccgggaa cgagattcaa ttgacagatg ctatcgatac actcaataaa
     1801 acacaacgcg tgtttgctcg tgaattcaaa ggcaaccgtt acgatgttgg ggataagttc
     1861 ggtttcatga aaacttctat cgactacgcc ctccaacacc ctcaagtaaa agatgatttg
     1921 aaacaatact tgatcgatct tggtaaaaaa ttagatacaa agaaaaacta gcaagcgcta
     1981 gttttttctt atgtcaaata aaatatccct aataataggg cgatcagacc cagatagcca
     2041 atgaaactga aaaaaatctg ttttcctgta aagagatctt tttccacaag aggtggaagg
     2101 aacatcacga ccaagcaacc tccaacagct cctccaatat gtcctgccat actaatggtt
     2161 ggattaaaaa gtcctaagac caagttcaat cctaaaagga ccatgtagcg ttgaccaaga
     2221 acctgtagat aagggctgcg actaaatttc ctcaaaagtg ccatagccgc aaaaagacca
     2281 aagagcgagg tcgatgcacc tgcaccaact acatctggtg taaaaaagag aacaaaaaga
     2341 ttgcccatta tcccagacaa gagatagagg agaaagaagc gccttgagcc aaacagtcct
     2401 tctaattgat aacccaaacc aagcaaagta atgctgttaa agataaaatg ctcccaacca
     2461 atgtggacaa agatggggga aatcaaccgc cacaactgag tcggatcata ccgcacaact
     2521 tgaccataca tgccaccaaa ttcaaaaatt gtataggctg tagtggtctg cccaaatcga
     2581 aatacttgta tcaaaagaaa aacaagagcc gtgactgcca acaagccatt ggtcacagga
     2641 tagcgcttat caaaaaagtt cttcaaaaat caacaactcc ttcactggaa tatcaaaaac
     2701 atctggttcg aaatcataga cttgaaagtc tgccaaggtc gataccgtct ccccctgata
     2761 atctgctagg tagcgatcat aaaaaccacc accatagccc actcgaaaac ccgcttgatt
     2821 ccaagctaaa ccaggcacat ggagcaactt aaccacagac ttatccacag gttgtgaata
     2881 aataccaggt tcccaaactc caaacctaga tttcaccaaa tcatcaggat tatattctac
     2941 aaaatccata cgtccttgtg aataggtctt agggatgaaa atctgcttgc cgtccttttg
     3001 agcctgttca atcaggtaag aagtgtcaaa ctcatggggc atggaaaggt aggtcccaag
     3061 cgactttgca ccctgataag tatcagaagc aaggaggcgt tccgttaact gctgactcca
     3121 tcttgctcgc tgactactag tcagatcttt caaactctgc aagacctctt gccgaatgct
     3181 ctttttatcc acagaaactc ctttcttttc agaaaaagac tggaaaacca gtctagtttg
     3241 ctttcatttt caaaaaatca ctcaacttat ccacagcaaa gggaagcact tcctccttgg
     3301 gactaaggcg aggattgtgg aggggataag gcgtatccac acccagccag aacataacac
     3361 ccggcacctt gttgagcagg tagccgaagt cctcacccgt catggcaggt ggacagtcaa
     3421 tcatctccac tccgtcaatg ccatcaaagt aggtcatgag ttcatccgcc aactgaggat
     3481 tattttccac aggtagataa ccgctaggat tgagctcgat ttccaagtct actccaaagg
     3541 acagggcgat tccctctgcc acttcccgca cccgcttttg caccagaaga ctcatgttct
     3601 gggtcaaagc ccgaatggtg ccgtgcaaaa aagctgtttc agcaataaca ttattggtcg
     3661 tgcccgcgtg catggagccg aaggtgacca ccgctccctc aatcggatca acgttacggc
     3721 tgaccaccga ctgcacctga gtcacaaagt agctggctgc caccaaggcg tcatttgcgg
     3781 tatagggaaa tgctgcgtgc ccaccctttc ctgtaaatcg tatcttgact tcacaggtcc
     3841 cagcaaagag ggtcgcacgg ttggtggcca tttgaccaac tttgaggtcc ggtcggacat
     3901 ggagcccata aaattcatct ggtaaccaat ccccgaaagc tcccgcttca tacataagca
     3961 tgccacctgc tagattttcc tccgcaggct gaaacaagaa gagaagatta tttctcggct
     4021 gctgctctgc cattttctcc aaaagcccca aggcaatggt catgtggaaa tcatgtccgc
     4081 aggcgtgcat cctgtctggg tggagggact tgaagtccag gcccgtctcc tccacgatcg
     4141 gcagaccgtc aatatccgcc cgccagccaa tcgttttctc tggagcagag cctgtcagat
     4201 agaccagaat ccctgtcttc caagtgcgga cttgggcaaa agaacagtct tgaagtaaac
     4261 cttcaatagt ttccataaga aaggcgtggg tcttgaactc ctccatgccc agctctggaa
     4321 tctggtggag ggcccggcgt gtcgcaatca aatctagcat agctacctct tagagtgtcc
     4381 gcaaagcctc ttccaaggct gttttttgtt gggtttgagc gtcgatttcc ttgatgatgc
     4441 gggctggcac acctgccacc actacgtttt caggaacatc ttgagtcaca atggcacccg
     4501 ccgccactac agaaccgcta ccgatttgca cgccctcgat aacaacggca ttggcaccga
     4561 ccaaaacgtt gtcgccgaca cgaactggct cggcagaagc tggctcgatg acacctgcaa
     4621 ggaccgcacc tgcaccgatg tggctgtttt taccaacggt cgcacggccg cccaaaatcg
     4681 cacccatatc aatcatggta cctggaccga tttccgcacc gatgttaatc accgcgccca
     4741 tcatgataac ggcattatcg ccaatggtca cttgatcgcg gataatggca cctggctcga
     4801 tacgggcatt gatattacgc ttgtccagca aaggcacagc cgaattgcgg ccgtcttgct
     4861 ccaccacata gtctacattt tcagtcaagt ttgccaagag tggctcgaca tccttccagt
     4921 caccgaagag gacattgcca agctttgtca cttcagcagg aacagctcct gccaattctc
     4981 cttcaaaggt cactttaaca gttgtcttct tcacagcatt accgataaaa gcaatgattt
     5041 cttgcgcagt cattttttgt gcagtcataa tgtttccaat ctaaaaataa tattttttct
     5101 attatatcaa aattttctga aaatttggga agaaatgcaa aaaaatgaaa aagggagtgg
     5161 gaaagaactc gattaggtca aaaaagagtt cgtcttccca ccctcgcaca gttgattagg
     5221 ccagatttgg agtgcaaaac acgaacaaat ctgccaatca accactgcgc tgagatgttg
     5281 atacgaactc tgagaagtga tcctgggctt tttgcccagc ctcctaaata ctatagaact
     5341 ctctatcctc ctaaagcagc tgacatttgc tctatctgct gttgcaaacc atctctatac
     5401 tctttcaaga atgtaataat ctctgctctt ccaacaattg cagcatttcc taatggacca
     5461 tattgttttt tctgttgctc aacagattgt gataccgtat cacgcacaga ctggtacata
     5521 gtggcatcac ccaatccata ctggctgacc atattttcat agttcgtaat atattcattc
     5581 acaatgaatt catacatctt catataatcg ctataagttt ttatactttc aattgttgca
     5641 tctgagctat ctgtcggatt aaattctgaa actgtagcag tactttctga acttgttggg
     5701 gtttccgtag ttttagagga agattctact acctcatcac taatatctac gctatttaag
     5761 atctgttcaa actcttgttt cctagtggaa gaatagtcat cctttgacat ataaattaca
     5821 tttaaaaatc cctgttggag tgtcgactaa aaagagttga cctttatatg cttcgccatc
     5881 tatcgtacct gtcgcatcat aaaggaagct gtccatccca tcaatcttag ctgagctttc
     5941 gttagctggt atcacttttc ctgactgttc catccccttc agatattggg agcgaacagt
     6001 tgcatcttta actgattcct ctaagggcaa ccaatatgcc attgccaatg atgaatcatc
     6061 gggataaaaa tataaggtat cactcgtatc cccttcttta ctcttccatg attcatttat
     6121 gctgatagcc acttctgaca attggtaagt tttgtatctt atagagcttg tacttacctg
     6181 ttctgtcgaa gattggccac cgctagagct cgtaaggttc ttaatcagac caatcgcaaa
     6241 caatccaacc aacacaataa accaaattcg cttgtaaaat ggtttctttt gcacatagac
     6301 attcccatgt tcatctctaa ttttcttcgc catataatcc tccaaaaaat tgtgtacaac
     6361 accattatat atatatatgc gttgtcaagt agaaaaacaa aaaaattaga agttttacct
     6421 cctaattttc atgtattatc ttaatcttca atttcttttc tgggattgac agctcctacg
     6481 atgtaatcct gtaccaagcc tgtaccatcc agcacgatcc catgcaaaac tccgccgtaa
     6541 acagctccac cgtcaatccc cattttccca tctggactgg tccagatttg gctaatgcgg
     6601 agttttgttt gataaaggtt ataaacagga gtatgcccaa agacaatcat cttccctgtg
     6661 gtattttcgg cctcatggaa aggggtgcgt aaccaaacct tatcatgatt gctggtttca
     6721 cgccagtctg gtaaggtcaa gtccacacca gcatggacaa agatataatc ttccgtttcc
     6781 agatgatagg gacaagattt gacgaaatca atcaggtcct catattgttc caacactgca
     6841 ttggcatcca ccaatccatc tacaggagca tccaagggcc gaccaagaag ggagttaatc
     6901 gtcgtatcgc caccatttcg ccgatagtgg tcatagcggt caatcggatc acgcaaccag
     6961 gctagaaaca ttcgctcgtg gttacccgtc agacaaattg cccccttttc ccgcaccaag
     7021 ctccagacca actccaaaca ggctttggaa ttttctccgc gatcaatcaa atcacctagg
     7081 aagatcaact gttgtcgctt ctcgttccat ttcttcaaaa tatcgagtag cagttcaaac
     7141 tttccatgga catcgccgat tacaaaaaat tcttttttca tagactctat tctaccacat
     7201 ttaataagca aagaaaaact gaccgtttcc gatcagtttg gttttcttac aaacgtgcgt
     7261 tcaattctgc accgagttct tcaaatcctg gttttccaag aagagcgaac atgtttttct
     7321 tgtaggcttc tacacctggt tggtcaaatg ggttgattgc attgaggtaa ccagagatcg
     7381 caattgccaa ctcgaagaag tagaaggtgt aaccaagtgt gaactcatct tgttgcggaa
     7441 gagtgacaaa catgtttggt acgccaccat ctgtgtgggc aagaagtacg ccgtccgttg
     7501 cttttttgtt tacaaagtca acgtcttttc cttgcaagta gccaagaccg tcaaggtctt
     7561 ctgccatttc aggaatcaag atattttttc ttggtttatc aacacgaact actgtttcaa
     7621 agatattgcg gtttccttct tggataaatt gtccaagtga gtgcaagtct gttgagaagt
     7681 tagcggatgt tgggttgata cctttttggt ccttaccttc tgactcgccc gccaattgtt
     7741 tccaccactc gctgaagtat tggagtgatg gctcgtagtt agccaagatt tctgttacat
     7801 agccttttct gtaaaggatg ttacggacag ctgcatattg gtatgcttgg ttttcagcaa
     7861 tcttatcaga tgtaaatgtc gtgcgggctg cgtttgcacc ttccataagg gcatcaatgt
     7921 ctgcaccagc tgccgcgatt ggcaaaagac caactgcagt caagactgag aaacgaccgc
     7981 cgacatcatc tggaactaca aaagtttccc aaccatttgc gtccgcttca accttaactg
     8041 caccttttgc cttgtcagtt gtcgcataga tacgcttgtt ggcttcttct tgaccgtatt
     8101 tcttgacaag taattctttg aagacgcgga aggcaatagc aggttcagtt gttgtacctg
     8161 attttgaaat cacgttaact gagaaatcct tatcagcaac ataatccacc aagtcagcaa
     8221 gatagcttga tgagatagag tttccagcgt aaaggatttg tggagctttg cgctcttcac
     8281 gtgtttgcaa gtttacaaag gcattgctca agaaatcaat agctgcttta gcaccaaggt
     8341 aagaaccacc gataccgata actaccaaaa cttcactttc attttggatt ttagctgctg
     8401 cttccttgat acgagcaaac tcttctttgt cgtagttttc tggcaaatcc aaccagccga
     8461 taaagtcgct accaggacct gttccttgac ggagcatttg gtctgcaagg gtaacttgtg
     8521 gttgcatgta ttctacttct tgagctccaa cgaattggcc caagactttt gaataatcaa
     8581 atgtaatatg tgtcatgttt tt
//
LOCUS       DBNFCK010000080         8487 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.080, whole genome shotgun sequence.
ACCESSION   DBNFCK010000080 DBNFCK010000000
VERSION     DBNFCK010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8487
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.080"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            13..1011
                     /locus_tag="AC1PIW_001404"
     CDS             13..1011
                     /locus_tag="AC1PIW_001404"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIP6606334.1"
                     /translation="MKVLLYLEGKTVLEKSGIGRALQHQMEALDMAGIPYTTDLLGDY
                     DVIHINTYGPRSWLLLHAAKRSGKKVIMHGHSTKEDFQNSFIGSNTLAPFVGKYLASM
                     YQKADFVITPSEYSKKLIQSYGVTTPIIAVSNGIDLDKYKKDERKEAIFREHFKIEEG
                     QPVVVCAGLYFRRKGIEDFVKVAEKMPHVRFIWLGSINKWLIPSYIRKIVEGDHPANV
                     EFPGYFKGAVFQGAMSGANAFFFPSYEETEGIVVLEALASHQHVVLRDIPVYDGWIDE
                     NVAELGHTVDDFVDSIQKILDKQVDKREAGYQVAVSRSIDNVSHQLVDAYRQVLEM"
     gene            1012..2325
                     /locus_tag="AC1PIW_001405"
     CDS             1012..2325
                     /locus_tag="AC1PIW_001405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIP6606335.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKLGHTVFIFTTTDED
                     VNRYEDWDIIRIPSVPFFAFKDRRVAYAGFTDALKIASRYKLDIIHTHTEFSLGILGK
                     MIAKELDIPVVHTYHTQYEDYVHYIAKGRIIRPGMVKYLVRAFLKDLDGVICPSEIVE
                     DLLMSYNVPISKRVIPTGIDLAKFDRPEIGKDETDELREQLGIASDETMLLSLSRISH
                     EKNIQAIVQAMPKILLENPKVKLVIVGGGPYTDDLQEMIAELNLDEHVQLTGMIAPSD
                     TALYYKAADFFISASTSETQGLTFLESLASGTPILAHSNPYLKNVITDKMFGTLFIRE
                     QELADAVVEAIVATPPMNPYVLDKKLYEISSTNFGRKVFEYYLDLKISYDFRKEHTNQ
                     DSVAEALIKEAIYLPQKVVVKSTDTTAKILKKSVEQVKSIRNFFE"
     gene            2340..2714
                     /locus_tag="AC1PIW_001406"
     CDS             2340..2714
                     /locus_tag="AC1PIW_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606336.1"
                     /translation="MEKLRKESWKNLVLATLIILSMYAGIYLIAFHLISDTMLSKELV
                     VGNILLYTPIVLFLTGLLYGFFHGFSPSLLVLVLLLYPVTIFFFKEWIILYQIVYTLC
                     ALIGNGIGALLFSLRKKMRKNP"
     gene            2973..3293
                     /locus_tag="AC1PIW_001407"
     CDS             2973..3293
                     /locus_tag="AC1PIW_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HIP6606337.1"
                     /translation="MLPDDLLRRCQDVRRAYRELEIKHHDKEWSIDEDLLALTNDIGN
                     MNRLIMTKQGRYYDETPYTLEHKMAENIWWLIELADRLDIDIQKEMETFLAQKEELLG
                     IKNK"
     gene            3307..5259
                     /gene="thrS"
                     /locus_tag="AC1PIW_001408"
     CDS             3307..5259
                     /gene="thrS"
                     /locus_tag="AC1PIW_001408"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIP6606338.1"
                     /translation="MIKITFPDGAVREYQAGVTTFEIAESISKSLAKKALAGKFNGKL
                     IDTTRAIDEDGTLEIVMPDHDDALDILRHSAAHLFAQAARRLFPDIKLGVGPAIQDGF
                     YYDTDNAAGQISNEDLPRIQEEMMKIVKENFPSERREVTKEEALEIFKNDPYKLELIH
                     EHSDDEGGLTIYTQGEYVDLCRGPHVPSTGRIQIFELLNVAGAYWRGKSENPMMQRVY
                     GTAWFDKKDLKAYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRT
                     LERYITDKELASGYQHVYTPPLASVELYKTSGHWEHYSEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVYKNHVRSYRELPVRIAELGMMHRYEKSGALTGLQRVREMTLNDGHIFVTP
                     EQIQEEFKKALQLIIDVYADFNLNDYRFRLSYRDPEDKEKYYDNDEMWENAQRMLKGA
                     MDEMGVDYFEAEGEAAFYGPKLDIQVKTALGNEETLSTIQLDFLLPERFGLTYIGADG
                     EEHRPVMIHRGVISTMERFTAILIETYKGAFPTWLAPTQVTMIPISVEAHLDYAWKVA
                     KELQDRGVRVHVDERNEKMQYKIRQSQTSKIPYQLIVGDKEMEDNAVNVRRYGSKATQ
                     TQSVSEFVDHILADIARKSRPAEAAE"
     gene            complement(5331..5990)
                     /locus_tag="AC1PIW_001409"
     CDS             complement(5331..5990)
                     /locus_tag="AC1PIW_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIP6606339.1"
                     /translation="MNYVLELLKPSTFQLLWSGLKLTLYISSISVILSILFGMILAIM
                     RNGKNPIFKWIATIYIEFVRNVPNLLWIFTVFLVFQMKSTPAGITAFTIFTTAAMAEI
                     IRGGLNAIGPGQTEAGLSQGFTPVQIMVYIIMPQAIRKMLPAIISQIVTVIKDTSFLY
                     SVIALQELFGSSQILMGRYFEAEQVFALYIMVALIYFSINFAISSLSRYVAKSWASSI
                     E"
     gene            complement(6005..6703)
                     /locus_tag="AC1PIW_001410"
     CDS             complement(6005..6703)
                     /locus_tag="AC1PIW_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIP6606340.1"
                     /translation="MTVLTTSPYAWENWVSYFKDFPIFFQGFLFTLAISVGAFIMAMF
                     LGIVFGSLSSSKNKVLKIIARVYVEYYQNTPLLVQFMIVYYGLPLISNYVLMPSIYWT
                     AVICVGLYHGAYIAEVIRAGIEAVPTGQTEAALSQGFTQSETMRIIILPQAIPTILPP
                     LTNQVVNLIKNTATVAIISGADIMFMTKSWSAMNANYIPAFAGAAFLYFIMCFPVASW
                     GRRIEEKNKSAFSH"
     gene            complement(6718..7555)
                     /locus_tag="AC1PIW_001411"
                     /pseudo
     CDS             complement(6718..7555)
                     /locus_tag="AC1PIW_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646622.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
     gene            complement(7566..8327)
                     /locus_tag="AC1PIW_001412"
     CDS             complement(7566..8327)
                     /locus_tag="AC1PIW_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIP6606341.1"
                     /translation="MALVEFKDVNKYYGDYHALRNINLEFEPGQVVVLLGPSGSGKST
                     LIRTINGLEAIDEGTLIVNGHDISSTSIKELVSLRKEVGMVFQHFNLYPHKTVLENVT
                     LAPIKVLGINKATAEKTAQKYLEFVNLWDRKDSYPAMLSGGQKQRVAIARGLAMKPEL
                     LLFDEPTSALDPETIGDVLAVMQKLARDGMNMIVVTHEMGFAREVADRIIFMAEGEVL
                     VDTTDVNGFFDNPTEPRAQQFLSKIINHESDKVKL"
BASE COUNT         2487 a         1626 c         1963 g         2411 t
ORIGIN      
        1 tataatggta ttatgaaagt actattgtat ttagaaggta aaactgtttt agagaaatca
       61 ggaattggcc gggcacttca acaccagatg gaagcgctag atatggcagg cattccttat
      121 acaacagacc tgctgggaga ttacgatgtc attcatatca atacctatgg tccacgctcc
      181 tggttattgc tacatgcagc caaacggagt ggaaaaaagg taatcatgca tggacattcc
      241 acgaaggaag attttcaaaa ttcctttatc ggttcgaata ccctagctcc ttttgtaggg
      301 aaatatctag caagcatgta ccaaaaggca gactttgtca tcactccttc agagtattca
      361 aaaaaactca tccagtctta cggtgtgaca acgccaatta tagccgtttc gaatggtatt
      421 gatttagata aatataagaa ggatgaacgc aaggaagcaa tctttcgaga gcattttaaa
      481 attgaagaag ggcagccggt tgttgtctgt gcaggactct actttcgacg taaaggaatt
      541 gaagactttg tcaaggtagc cgaaaagatg ccgcatgtcc gcttcatttg gctcggttca
      601 atcaataagt ggctgattcc gtcatatatt agaaaaattg ttgagggaga tcatccagcg
      661 aatgtcgagt ttccaggtta ttttaaaggt gctgtttttc aaggtgcaat gagcggagcg
      721 aatgcctttt tcttcccatc gtatgaggaa acagaaggga ttgttgttct ggaagctctt
      781 gctagtcatc agcatgttgt tctaagagat attccagttt acgatggttg gattgacgaa
      841 aacgttgccg aattgggaca tacagtggat gattttgtcg attctattca gaaaatctta
      901 gacaagcagg ttgataaacg tgaggcgggc taccaggttg cggttagtcg ctcgattgac
      961 aatgtttccc accaattggt ggatgcctat cgacaagtat tggagatgta aatgcggatt
     1021 ggattgttta cggataccta ttttccacag gtttcaggag tcgcgacttc gattcggact
     1081 cttaagacag agttggagaa attgggacat acggtattta tttttacgac gacagatgag
     1141 gatgtgaatc gctacgaaga ttgggacatt atccgaattc ctagcgtacc tttttttgcc
     1201 tttaaagatc gtcgagtggc ctacgcaggt tttacagatg ccttaaaaat tgcaagtcgc
     1261 tataaactag atattattca tactcataca gaattttctt tggggatttt ggggaaaatg
     1321 attgccaagg aactagatat tccggtggta catacttatc acacccagta tgaagactac
     1381 gtacactata ttgccaaggg gaggattatt cgtcccggga tggttaaata tttagtacgg
     1441 gcatttttaa aagatttgga tggggtaatt tgtcctagtg agatcgttga agatctttta
     1501 atgagttaca atgttccgat ttctaaacga gtaattccga caggaattga cttggcaaag
     1561 tttgatagac cagaaattgg caaggatgaa acagatgagt tgcgggaaca gctaggaatt
     1621 gctagtgatg aaacgatgtt gttaagtctg tctaggattt ctcacgagaa gaatatccaa
     1681 gcaatcgttc aggcaatgcc aaaaattttg ctggaaaatc caaaggtgaa gttggtcatt
     1741 gtcggaggtg gaccttatac agatgatctt caggaaatga tagcggagct taatctagat
     1801 gaacatgtcc agttgacggg aatgattgca ccaagtgata cagcacttta ttataaagct
     1861 gcggatttct ttatttctgc ttcgaccagt gaaacacagg gactaacttt cttagaaagt
     1921 ttagcttctg gaactcctat tttggcacat agcaatccct acttaaaaaa cgttataaca
     1981 gataagatgt ttgggacgct cttcattcga gaacaggagt tggcagatgc cgtcgttgag
     2041 gcaattgttg ctacaccacc aatgaatccg tatgtgcttg ataagaagct ctatgagatt
     2101 tcttctacga attttggacg taaggttttt gaatattatc tagatttgaa gatttcgtat
     2161 gattttagaa aagaacatac taatcaagat agcgtagcag aagctttgat aaaagaggct
     2221 atttacctac cgcaaaaagt agtagttaaa tcaacagaca cgacagcaaa aatattgaaa
     2281 aaatcagtcg aacaagtaaa atcaatccgg aatttctttg aataatttgg aggtttcaaa
     2341 tggaaaagct tcggaaagag agttggaaga atctggtttt ggcaactctt attattctgt
     2401 ccatgtatgc aggtatttat ctgattgcct ttcatctgat tagtgatact atgctttcta
     2461 aggaactggt ggtcggaaat atattgctct atactcccat tgtgctattc ttaactggtc
     2521 ttctttatgg attttttcac ggtttttctc ctagccttct tgtcttggtt ttactacttt
     2581 atcctgtaac catctttttc tttaaggaat ggattattct ctatcagatt gtctacacct
     2641 tatgtgctct gattggaaat gggataggag ctctgctatt ttctctaaga aagaaaatga
     2701 ggaaaaatcc ttgaaaaatc aggaaatcct gttataatag cttttagaga cttatcaatt
     2761 ctaggaaatc tttgagagag tgtgcggtgg gtgcaagcac atagaggata ggattgacac
     2821 tactcggtat gattttatgc aaacataaaa gggtggttcc cttatcgcca atctgaggta
     2881 gtcaggcttt tttgcgtgac tgcgaatgaa ggtggaacca cgttacgacg tccttttacg
     2941 agggcgtcgt attttctttt ggaggaggaa tgatgctacc tgatgactta ttaagacggt
     3001 gtcaagatgt gcgtcgtgct tatagagagt tggaaatcaa gcaccatgac aaagagtggt
     3061 ctattgatga ggacctcttg gctttgacca atgatattgg caacatgaac cgactgatta
     3121 tgaccaagca gggtcgttac tatgacgaaa cgccttatac cttggagcat aaaatggctg
     3181 aaaatatctg gtggttgatc gaattagccg accgcctgga tattgatatt caaaaagaaa
     3241 tggaaacctt tttagctcaa aaagaagagt tattggggat taaaaataaa taaaaaggag
     3301 ataaacatga ttaaaattac tttcccagat ggtgctgttc gcgagtacca ggctggtgtg
     3361 actacttttg aaattgcaga gagcatcagc aagtctttgg caaaaaaggc tcttgctggt
     3421 aagttcaatg gtaaattgat tgacactact cgtgccattg acgaggacgg cacgcttgaa
     3481 attgtaatgc ctgaccacga cgatgccttg gacatcttgc gtcactcagc agctcatttg
     3541 tttgcccaag cggctcgtcg ccttttccca gacattaagt tgggcgttgg tccagccatt
     3601 caggacggtt tctactatga tacggacaat gcggctggtc agatttcaaa tgaagatttg
     3661 ccacgcattc aggaagaaat gatgaagatt gtcaaggaaa acttcccttc tgaacgtcgt
     3721 gaagtgacta aggaagaagc tcttgaaatc ttcaaaaacg atccttacaa gttggaattg
     3781 attcacgagc actctgatga cgagggcggt ttgaccatct atacgcaggg tgaatacgtt
     3841 gacctctgtc gtggtccaca cgttccgtca acgggtcgta tccagatttt tgaattgctc
     3901 aatgtggctg gtgcttactg gcgtggtaag agtgaaaatc caatgatgca acgtgtttat
     3961 ggtacagctt ggtttgacaa gaaagacctc aaggcctacc ttcaaatgcg tgaagaggct
     4021 aaagagcgtg accaccgtaa acttggtaag gaattggatc tcttcatgat cagccaagaa
     4081 gttggtcaag gtttgccatt ctggttgcca aatggggcga ctatccgtcg taccttggag
     4141 cgttacatca cagacaagga attggcttct ggttaccagc acgtttacac accaccattg
     4201 gcttctgttg aactctataa gacttcaggt cactgggagc attactcaga agatatgttc
     4261 ccaactatgg acatgggcga cggtgaagag tttgttcttc gtccgatgaa ctgtccacac
     4321 cacattcaag tttacaaaaa ccatgtgcgt tcttaccgtg agttgccagt gcgtatcgct
     4381 gagcttggta tgatgcaccg ctatgaaaaa tctggtgcat tgactggttt gcaacgggta
     4441 cgtgagatga ccctcaacga cggtcatatc tttgtgacac ctgaacaaat ccaagaagag
     4501 ttcaaaaaag ctctccagtt gattattgat gtgtacgcag atttcaacct caacgactac
     4561 cgtttccgcc tgtcttatcg tgacccagag gacaaagaga agtactatga caacgatgag
     4621 atgtgggaaa atgcccagcg gatgttgaaa ggtgccatgg atgaaatggg cgtggactac
     4681 tttgaagcag agggtgaagc agccttctac ggtccgaaat tggatattca ggtgaaaact
     4741 gcccttggta acgaggaaac cctgtcaacc attcagttgg acttcctctt gccagaacgc
     4801 tttggcttga cctatatcgg tgctgacggt gaggagcacc gcccagttat gattcaccgt
     4861 ggtgttatct cgactatgga acgtttcact gctatcttga ttgaaaccta caagggtgcc
     4921 ttcccaactt ggttggctcc aacacaagtg accatgattc caatctctgt ggaagctcac
     4981 ttggactacg cttggaaggt tgccaaagaa ttgcaagacc gtggcgttcg agtacacgtg
     5041 gatgaacgca acgagaagat gcaatacaag atccgtcaga gccaaaccag caagattcca
     5101 taccaactca tcgtgggtga caaggaaatg gaagacaatg ctgtcaacgt tcgtcgctac
     5161 ggaagtaagg caacacaaac tcagtctgta tcagaatttg tggaccatat cctagcagat
     5221 attgcccgca agtcacgtcc agctgaagca gctgaataaa taaagcaaga agtccgaaag
     5281 ggcttctttt ttgacagcaa aaactcccaa ccgattggct gggagaaatt tcattctata
     5341 ctgctcgccc aagacttagc cacataacgt gacaggcttg aaatagcaaa gttgatactg
     5401 aagtaaatca gggcaaccat aatgtagaga gcaaagactt gctccgcctc aaagtagcgt
     5461 cccatgagga tttgactcga accgaagagt tcttgcaaag cgataacgga gtataggaaa
     5521 ctggtatcct tgataaccgt cacaatctga gagatgatgg cgggcagcat cttgcgaatg
     5581 gcctgtggca tgatgatata gaccatgatt tgcactgggg taaaaccctg tgacaaacca
     5641 gcttcggtct ggcccggtcc gatggcgttg agaccaccac ggataatctc ggccatggct
     5701 gccgtggtaa aaatagtaaa agcagtaata ccagcaggcg ttgacttcat ttggaagact
     5761 aagaagacgg tgaaaatcca cagcaagttt ggtacattac gcacaaattc gatatagatg
     5821 gttgcaatcc acttgaaaat tggatttttc ccattgcgca tgatggcgag aatcattccg
     5881 aaaagaatgg acaggattac cgaaatgctt gagatataaa gggttaactt caatccgctc
     5941 cacaagagtt ggaaggttga aggtttgagt aattctaaaa cataattcat cgttcaacct
     6001 ccttttagtg tgaaaaggct gacttattct tttcttcaat gcgacgtccc cagcttgcga
     6061 cggggaagca catgatgaag taaaggaaag cagcaccagc aaaggctggg atgtagttgg
     6121 cattcatggc agaccaagat tttgtcataa acataatatc agctccagag ataatggcaa
     6181 cagtagccgt attcttaatt aagtttacta cttggttggt caaaggtgga agaatggtcg
     6241 gaatggcctg tggtaaaatg atgattcgca tggtttcaga ctgagtaaat ccttgtgaaa
     6301 gtgcggcttc agtttgccca gtaggcacag cctcaatccc tgcacgaatg acctctgcga
     6361 tataagcacc gtgatagagt ccgacgcaga taacagctgt ccagtagatg gatggcatga
     6421 gaacataatt tgaaatcagt ggaagtccat aataaacaat catgaactga accaaaagcg
     6481 gcgtgttctg atagtattca acatagacac gtgcgatgat ttttaaaact ttatttttac
     6541 ttgaagagag actaccaaag acaattccta aaaacatggc catgataaag gctccgacag
     6601 aaatggctaa ggtaaagaga aaaccttgga aaaagattgg aaaatctttg aaataactga
     6661 cccaattctc ccaagcgtaa ggactagttg ttaaaactgt catattgttt cacctttcta
     6721 ttcgtcagtt tctgtgactg gtttgagacc attggcatca tagatgcttt gtaggctacc
     6781 atcggcagtc catttttcta cgagactatt taagtagttg ttaaggtctt tgttagataa
     6841 tttagtaaca attccatagt cagaagctga gaagctaaag tccatgagtt cagatttcga
     6901 actgatatac ccagcaagga tagattggtc tacagaaaat gcgtctgtac ggtgagcacg
     6961 tagcgagaca gagagttctg gataagaacc aagctctgca aaattgacag tgataccgta
     7021 tttgtctgct agttcagaga tgagggtacg tgtaatggaa ccctgagcta caccgattgt
     7081 ctttccatcc aggtcggtga atgtcttaat tccactatcc ttattgacca agaagccaac
     7141 ggcatctgtg taataaggag ttgtgaaatt gtagagcaac ttacgttcct cagtgatggt
     7201 aaaaggctat gaccatatcc acttgactgt tatcaagaag tggtcctcgg gtttgcgctg
     7261 taacgggagt aaattcgata tctacaccta attcgtctgc aattttgcga gcaatatcaa
     7321 tctccattcc ctcgaattca ccagtatcag ggttcttgta gccaaagttt gggacgtcct
     7381 gtttcactcc gacgcggagc acgcctctat cgatgatagc ttgaacttga tcactattag
     7441 taatggatgc gggtgtttct gtttgagcta ctggttgttt tgcgaaaacg agaagaataa
     7501 gtccaaagac caataatcta attgcaaaga aaatgttgat ttttttctta tccatagact
     7561 tcctcctaga gttttacttt gtcagattcg tggttgataa tcttactgag gaattgttgg
     7621 gcacgaggct ctgtcggatt gtcaaagaag ccattcacat ctgttgtatc aactaaaact
     7681 tctccctcag ccatgaagat gatgcgatca gctacttcac gggcaaagcc catttcgtgt
     7741 gttacgacaa tcatgttcat cccgtcacgt gccaattttt gcataactgc aagtacatct
     7801 ccgatagttt ctgggtccaa ggcagatgtt ggttcatcga agagtagaag ctcaggtttc
     7861 atagctaagc cgcgagcgat ggcaacacgt tgtttttggc ctccggatag catagcaggg
     7921 taggaatcct tccgatccca gaggttaaca aattcaagat acttttgagc ggttttttca
     7981 gctgtagcct tattgattcc caaaacctta atcggtgcca aggtaacatt ttctaacacg
     8041 gttttgtgtg ggtaaaggtt gaaatgctgg aaaaccatgc caacttcttt gcgaagtgat
     8101 acgagctctt tgatagaagt agaggaaata tcatgtccat tgacgatgag cgttccttca
     8161 tcaattgcct ctaaaccgtt aatcgtgcga atgagagtag attttccgga tccggaaggt
     8221 ccaaggagaa cgacaacctg tccaggttcg aattccaagt tgatattgcg tagggcgtgg
     8281 tagtcaccat agtatttatt gacatcttta aattctacta aagccataaa attctccttg
     8341 catactgttt tttaggtatt cccatcttag cattgcctat atacaatgtc aagtaaattc
     8401 tgacaattta atgtgttgta tgttataaaa tgtgacatca ccgtgtcaga tggttttgaa
     8461 atatgaattt tttaggtaag tctgata
//
LOCUS       DBNFCK010000081         8432 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.081, whole genome shotgun sequence.
ACCESSION   DBNFCK010000081 DBNFCK010000000
VERSION     DBNFCK010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8432
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.081"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(485..1315)
                     /locus_tag="AC1PIW_001413"
     CDS             complement(485..1315)
                     /locus_tag="AC1PIW_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIP6606342.1"
                     /translation="MKKMMLGALALVTSLTLAACGSNDSATKSDNWTAYESEKSVTIG
                     FDKTFVPMGFEQTDGSYTGFDIDLANAVFEKYGITVKWQPIDWDLKETELNNGNIDLI
                     WNGYSITDERKEKVLFTNPYMDNQQVLVTKKSSNISQVSDMKDKILGAQAGSSGYSVF
                     ESQPAILKDIVQNNDASQYATFNEALIDLKNDRIDGLLIDRVYANYYLQQEGIIADYN
                     IIDAGFENEAFAVGARKSDTTLVENINKAFAELYKEGKFQEISQKWFGEDVATDTVKK
                     "
     gene            complement(1342..1974)
                     /locus_tag="AC1PIW_001414"
     CDS             complement(1342..1974)
                     /locus_tag="AC1PIW_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIP6606343.1"
                     /translation="MLELRNLSKRFEDKQIFSNYDLVIPEGKIVAIVGQSGGGKTTLL
                     RMLAGLETIDSGTLMYNGQELPLEELGKRHLLGFVFQDFQLFPHLSVLENLVLSPMKT
                     QNMLRSEAEDKALKLLDTLGLANHANAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRKEVEKLILENRATGITQIVVTHDMQFAENIADEIIKIEPKH"
     gene            complement(1984..2625)
                     /locus_tag="AC1PIW_001415"
     CDS             complement(1984..2625)
                     /locus_tag="AC1PIW_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004228788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIP6606344.1"
                     /translation="MDYVLEVLPSLLNGAAVSLQVFFLVLILSLPLGAVFAFLMQIKF
                     KPLQWLLHFYVLIMRGTPLLLQLIFVYYVLPSVGITFDRMPAVILAFTLNYAAYFSEI
                     FRGGIEAIPKGQYEAAKVLKFTPVQTIRYIVLPQVVKIVLPSVFNEVMTLVKDTSLVY
                     ALGVSDLLLASRTAANRDASLAPMFIAGAIYLLMIGAVTLISKQVEKKFDYYR"
     gene            complement(2777..4588)
                     /gene="glmS"
                     /locus_tag="AC1PIW_001416"
     CDS             complement(2777..4588)
                     /gene="glmS"
                     /locus_tag="AC1PIW_001416"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIP6606345.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVTGGEQAHL
                     VKAVGRIAELSAKVGDKTEGTTGIGHTRWATHGKPTENNAHPHTSQTAGHILVHNGVI
                     ENYAEIKEEYLAGHDLKGQTDTEIAVHLIGQFAEEGLSTLEAFKKALKIIQGSYAFAL
                     IDAADADTIYVAKNKSPLLIGLGDGYNMVCSDAMAMIRETSEYMEIHDKELVIVKKDS
                     VEVMDYDGNAIERGSYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLISAYTNEAGQ
                     VTVDADIIKAVQEADRIYILAAGTSYHAGFAAKDFLEKLTDTPVELGISSEWGYSMPL
                     LSKKPLFVMISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREATYTMLLHAGP
                     EIAVASTKAYTAQIATLAILAKAVGDANGNAYAKEFDLVHELSIVAQSIEASLSEKDV
                     IAEKVEKLLSTTRNAFYIGRGSDYYVSMEASLKLKEISYIQCEGFAAGELKHGTISLI
                     EDGVPVLALISNHPHLASHTRGNIQEVVARGANVLTIVDEAVAKEEDDITVTTVHPFL
                     SAIAMVVPTQLIAYYATLQRGLDVDKPRNLAKSVTVE"
     gene            complement(4778..5320)
                     /gene="lepB"
                     /locus_tag="AC1PIW_001417"
     CDS             complement(4778..5320)
                     /gene="lepB"
                     /locus_tag="AC1PIW_001417"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIP6606346.1"
                     /translation="MVKRDLIKQISLLVLLIFGIIGLRFWLLEPVTITPEMANSYLKE
                     NDFIMTVRNVRPIHGDFILYNHEGKEYVSRVIALENETVTYMDDVLYRNDIIVTENYL
                     KTPHSQESYTDDFTLETLTNGKYNIIPEGHYLVLNDVRTNQQDSRSFGLISSEAIVGR
                     LTFRISPLSEFGFIKTGLVQ"
     gene            complement(5363..5980)
                     /locus_tag="AC1PIW_001418"
     CDS             complement(5363..5980)
                     /locus_tag="AC1PIW_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HIP6606347.1"
                     /translation="MSDIMYPEVLTVGSGAVKVATVGDSLTYGYGLENREKDAYPCIL
                     AEKLGHHYQVSNYGLSGRSLQSTADFPYFKEKNAQLSLESEADIVIIMIGSNDSRGPY
                     WNRERFIQEYREMAEQYMDLPSQPDVYLVIPPYVPTSRFGLNNQIIEDELQKIIPAIG
                     NELGLPVINLYTVTEGHLEYYSDGLHLTPLGNQVIAEEIYQHLRH"
     gene            6065..7174
                     /locus_tag="AC1PIW_001419"
     CDS             6065..7174
                     /locus_tag="AC1PIW_001419"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIP6606348.1"
                     /translation="MKKQVIIIGGGIVGSTAAFYLSQEEQVNLTLIDSGVGTATRAAA
                     GIIGPWMAQKKNKDWYKLTSDGAVFYRQLVADLEKSGATEIPFKQTGTIGLKSKPELL
                     DKIQKIAEERRVDTPTIGEITPLIGSEINQYLPLLKPDYFGIHLEGGGRIDGGRLIDI
                     LQEQFLKNGGTRIYGQAKLLDNHTVEVDNQVLTADHIILATGAWLPHILEPLGYQVDV
                     RPQKGQLFELDTEFDTDNWPVCMPYGEIDILPFENGKIIIGATHEDDMGYDLELDPEK
                     IQAMHDKIAEFMPDLANYPVTRTRIGTRAYTSTYSPFYGNIEDMPNVWVASGLGSTGL
                     TNGPIIGWQIAKEILNQETNFDRTPYSPNRYILKK"
     gene            complement(7499..>8432)
                     /locus_tag="AC1PIW_001420"
     CDS             complement(7499..>8432)
                     /locus_tag="AC1PIW_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="host-specific IgM cysteine endopeptidase
                     Ide-Ssuis"
                     /protein_id="HIP6606349.1"
                     /translation="ESVATPLEKDSIQAGETEIAEPTSSESTSVEAELVDNSEIHAAT
                     SSVTPCGSSAYADGSTTESVATPLEKDSIQTGNTEIAEPTSSKSTNVEAASVDNSEIH
                     ADASLTAVSSVNLDNPVIEPVAISLIGSKRDTNAEVEVSSLSKREVRKTNTDGLISVQ
                     SKVIKKELLESSLAEAGSPLLEATIAQSSNSNSTEIGMSYQNTVLLESNNTERQVSKA
                     EIVMEHKETELVETVSSASEPVVLVENISQTSNNTIESGKNMGVQSQAGAKQILGVEQ
                     SSKVSTPTSRQIMGVGLLTLVLGSALGLLKKRRK"
BASE COUNT         2561 a         1814 c         1495 g         2562 t
ORIGIN      
        1 actactaggg acgaactagt agcctaaata caaatttttt tcactttgat tatatcatag
       61 gaagggaatt tttgcattct tttgagtttt cgtataagaa ctcgaccaga cgaaaaaaag
      121 ttcgtcaaca atttttcgtt ttaagttgtt gagctgaaac aatctatccc cagacatcaa
      181 tttatgtgag ctgactgccg tcagcttata tctcccactt taaaaggtct cccagacctt
      241 ttaaactccc acccccgcat agctccaaca gtcagaatag tgactgttta agccaatcaa
      301 ccactgcgct gagatgttga cacgaactcc gagaagtggc gctgagcttt ttgctcagcc
      361 tcgtagaatc ttaagttaat gatattggtg tacaccccac cgttttcacg agttcttttc
      421 attattctgt ggatgagggt gtaaccatca tggatgtctt gttcagtcac ttttgcttac
      481 attattattt ttttactgtg tcggttgcaa catcctcacc aaaccatttt tgggagattt
      541 cttggaattt accttcttta tataattcag caaacgcctt attaatgttt tccacgagtg
      601 tcgtatctga tttacgagca ccgactgcga aggcttcatt ttcaaaacct gcatcaatga
      661 tgttgtaatc tgcgataatt ccctcttgtt gcaagtaata atttgcatac acacggtcaa
      721 tcaaaaggcc atcaatacga tcgtttttca agtcaatcaa ggcttcattg aaggtcgcat
      781 actgacttgc atcgttattt tgaacaatat ctttcaaaat agctggttgt gattcaaata
      841 ctgaatagcc agatgaacct gcttgcgcac ctaaaatctt atccttcata tcagatactt
      901 ggctaatatt ggaagatttt ttggttacaa gaacttgttg gttatccatg tagggatttg
      961 tgaaaagaac tttttcctta cgctcatctg taatagaata accattccaa attaaatcaa
     1021 tattcccgtt attcaattca gtttctttca agtcccagtc aattggttgc cattttacag
     1081 taattccata tttttcaaag actgcattag ccaagtcaat atcaaaacct gtgtaagaac
     1141 catctgtctg ttcaaatccc attgggacaa aggtcttatc aaagccaatc gtaacggatt
     1201 tttcagactc gtaggctgtc caattatcag acttagtagc cgaatcattg gagccacaag
     1261 cagcaagagt caaagaagtt acaagagcaa gagctccaag catcattttt ttaatattca
     1321 tcatcatatt tgttcctttt cttagtgttt cggttcaatt ttgatgattt catctgcgat
     1381 attctcagca aactgcatat catgggtgac cacaatttgc gtgattccag tagccctatt
     1441 ttcaagaatc agtttctcaa cttcttttct taattcaggg tctagggccg aagttggttc
     1501 atcatagccg ataatctcag gatcaatcat catcgcacgc gccaaggcca cacgttgttt
     1561 ctgtccaccc gataatgaaa atgggtaggc atttgcgtgg ttagcaagtc caagagtatc
     1621 caagagttta agggccttgt cttccgcctc tgaacgcaac atattttgcg ttttcatagg
     1681 agagagaacc aagttttcca atacggatag gtgcgggaaa agctggaagt cctggaagac
     1741 aaagcccagc aaatgtcgtt ttcccaactc ctctaatggc agttcttgac cgttatacat
     1801 caaagttcct gaatcaatag tctctaaacc agccaacatc cgtaaaagag ttgtcttacc
     1861 accgccagat tgaccaacaa tggcaacaat tttcccttct ggaatgacca aatcatagtt
     1921 ggagaaaatc tgtttatctt caaagcgttt tgacaaattg cgtaactcta acatgacttc
     1981 ctcctatcta taataatcaa atttcttttc tacttgttta gaaatcagtg ttaccgcacc
     2041 gatcatcaat aggtagattg cacctgcgat aaacataggt gccaagctgg catcacgatt
     2101 ggccgcagta cgactagcca acaacaaatc gctaacccca agtgcataga ccaaagaagt
     2161 atcctttacc aaagtcatga cttcattgaa aacacttggc agaacaattt tcacaacctg
     2221 tggtaagaca atgtagcgaa tggtctgaac aggggtaaat ttcaatacct tagcagcttc
     2281 atactgacct tttggaatag cttcaatacc accacggaaa atttcagaga aataggccgc
     2341 atagttgagc gtaaaggcca gaatcacagc aggcattcgg tcaaatgtaa tacccacact
     2401 tggtaagacg taataaacaa aaattaattg taaaagtaag ggggttcctc gcataattaa
     2461 tacatagaaa tgcaataacc attgtaaggg tttaaattta atctgcatta aaaaagcgaa
     2521 aacagctcct aatggcaagg ataatatcaa tactagaaaa aatacttgca aggagactgc
     2581 agcaccattt agtagactgg gcagaacttc caaaacataa tccatagagc tctcctaaaa
     2641 gaattattag gttctagtat atcaaaaaat atccttaatt tcaaagaatt tttcagaatt
     2701 tttaataaat ttaacaaaat tttgtatgtt ttttcattga tttgaaaaaa aggtccgaaa
     2761 tccgaacctt atcttcttat tcaacagtaa cagacttagc caagttacgt ggtttatcaa
     2821 catccaaacc gcgttgcaat gtcgcatagt aagcgatcaa ctgtgtcgga acaaccatag
     2881 caatcgctga taagaatgga tgaaccgttg taacagtgat gtcatcctct tccttagcta
     2941 ctgcttcatc tacaattgtc aagacattgg caccacgcgc caccacttcc tgaatattgc
     3001 ctcgagtatg gcttgcaaga tgtggatgat ttgaaatcaa ggccaagaca ggaacaccgt
     3061 cttcaatcaa tgaaatggta ccgtgtttca attcacctgc cgcaaagcct tcacattgga
     3121 tgtatgaaat ttctttcaat ttcaagctgg cttccataga aacgtagtag tcactaccac
     3181 gaccgatata gaatgcgttg cgagtggttg ataagagttt ttcaactttt tcagcaatca
     3241 catctttttc tgacaagctg gcctcaatag attgagctac gatggacaat tcatgcacca
     3301 agtcaaattc tttcgcgtag gcattgccat ttgcatcacc gacagccttc gccaagattg
     3361 ccaaagtcgc aatctgtgca gtgtaggctt ttgtagaagc taccgcaatt tctggtcctg
     3421 catggagcaa catggtgtaa gttgcctcac gtgatagggt cgaaccagga acgttggtta
     3481 cagtcaaact tggaatgccc atttcattag ctttaaccaa tacctgacgg ctatcagcag
     3541 tttcacctga ctggctaatc atgacaaaga gtggcttttt gctcaaaagt ggcatactgt
     3601 agccccactc agatgaaatt cccaactcta ctggtgtatc tgtcaatttt tccaagaagt
     3661 cttttgcggc aaatcctgcg tgataggaag ttccagcagc caagatatag atacggtcag
     3721 cttcttgtac tgccttaata atatccgcgt caactgttac ctgaccagct tcatttgtat
     3781 aggcactaat caatttacgc ataacagttg gctgctcatc aatttccttg agcatgtagt
     3841 atggataagt tcccttacca atatctgaca aatcaagctc agctgtataa ctaccacgtt
     3901 caatcgcatt gccatcatag tccatgactt caacactatc ttttttcacg ataaccaatt
     3961 ccttatcgtg aatttccatg tattcacttg tctcacgaat catagccatg gcatctgagc
     4021 agaccatgtt atagccatca ccaagaccaa tcaaaagtgg agatttattt ttggcaacat
     4081 agattgtatc tgcatccgca gcatcaatca aagcaaaggc atatgaacct tgaataatct
     4141 tcaatgcttt tttaaaggct tctagagttg acaaaccttc ttctgcaaat tgaccaatca
     4201 agtgtacagc aatttctgta tctgtctgcc ctttcaagtc atgacctgca agatactctt
     4261 ccttgatttc tgcgtagttt tcaatcacac cgttgtgaac taggatatgt ccagcagttt
     4321 gagaagtgtg agggtgagca ttgttctcag ttggcttacc atgagtcgcc caacgagtat
     4381 gaccgatccc tgttgtccct tcagtcttat cgccaacttt tgcagacaat tctgcaatgc
     4441 gaccaacagc tttcacaaga tgagcttgct caccacccgt cacaaaaata cctgctgaat
     4501 cataaccacg gtattctaat ttttcaagtc cttgaatcaa aatatcagtt gcgtttgtat
     4561 ttcctacaac accaacgatt ccacacatac atatcaccta cgatgtttgt taacatcggc
     4621 tttcatttct ttaaaaattg gtctagtcta atttatatct aaaaagacca gtgaaatcag
     4681 tatataacaa gatttcatcc ttgtcaacat tttaattggt ctagtctaat ttcaaaatac
     4741 agttccagcc atctcaatac aataaaaaaa gctgattcta ctgaaccagc cctgtcttaa
     4801 taaaaccaaa ttctgaaagt ggactaatcc gaaaagtcag tctaccaaca atagcctcac
     4861 tagaaatcaa accaaaacta cgactatcct gttgattcgt tcgtacatca ttcaacacca
     4921 aatagtgtcc ctctggaatg atgttgtact ttccatttgt caaggtttcc aatgtaaagt
     4981 catctgtata actttcttgt gaatgaggcg tttttaaata gttctctgta acaataatat
     5041 cattacgata gagaacatcg tccatatagg taaccgtctc gttttccaat gcaatcacac
     5101 gactcacata ctcttttccc tcatggttat acaaaataaa atctccatga atcgggcgga
     5161 catttcgcac tgtcatgata aagtcatttt ccttcaaata actatttgcc atctcagggg
     5221 taatcgtgac tggctcaagt aaccaaaatc gtaggccaat gattccaaaa atcaataaga
     5281 ccaataaact gatttgtttt attaaatctc tttttaccat aatctctcca tttcactagt
     5341 attataccac aatttagagc cattagtgtc tgagatgctg gtaaatctct tcagcaataa
     5401 cttgatttcc taacggtgtc aaatgaagac catcgctata gtattcaaga tgtccctccg
     5461 tcacagtgta aaggttaata actggtaaac caagttcgtt tccgatagct gggataattt
     5521 tttgtaattc atcttcaata atttgattat tcaatccaaa acgactggtt ggtacgtaag
     5581 gcgggatgac taaataaaca tccggctggc ttggtaaatc catatactgc tctgccattt
     5641 ccctatactc ttgaataaac ctctccctat tccaatatgg tcctcggcta tcgttactac
     5701 caatcatgat gatgacaata tctgcttcac tctcaagtga aagttgggca ttcttctctt
     5761 taaagtaagg aaaatctgca gttgactgaa gtgaacgccc actcaatcca taatttgaaa
     5821 cttgatagtg atgcccaagt ttctcagcta gaatacaagg ataggcatct ttttcccgat
     5881 tctccaagcc atatccataa gtcaaactat ctccgactgt tgcaactttt acggccccac
     5941 ttccaacagt taggacctca ggatacatta tatctgacac acgaactcct caatttttct
     6001 tattttctat tgtatcatgt tgaaaaaaat aaataaactt ctgaatttgt gatataatta
     6061 acttatgaaa aaacaagtta ttattatagg cggaggaatt gttggctcta ctgcagcctt
     6121 ttatctctcc caagaagaac aagtcaatct tactctcatt gattcagggg tcggaacagc
     6181 cacacgagct gccgcaggta tcatcggccc atggatggct cagaaaaaaa ataaagactg
     6241 gtataagctg acttccgatg gtgctgtttt ctaccgtcaa ttggtagccg atttggaaaa
     6301 atcgggtgct actgaaattc cattcaaaca gactggaacc atcggcctaa aaagtaagcc
     6361 tgaactactt gataaaatcc aaaaaattgc cgaggaacgt cgcgtagata ctccgactat
     6421 tggcgaaatt actccgctta taggctcgga aattaaccag tacctgccac tacttaagcc
     6481 agactatttt ggtatccatt tagaaggtgg cggtcgtatt gatggtgggc gtttgattga
     6541 tattcttcag gaacaatttt tgaaaaatgg tggtacacgt atctacggac aggcaaaact
     6601 gctcgacaac catactgtcg aagtagataa tcaagtgctt actgctgacc atattatctt
     6661 agccacaggg gcctggttac cacatattct tgaacctctt ggctatcaag tggatgttcg
     6721 accacaaaaa ggacaattgt tcgaattaga tacagaattc gataccgaca attggccagt
     6781 ttgtatgcct tacggagaga ttgatatcct accatttgaa aatgggaaaa tcattattgg
     6841 tgcaactcat gaagatgaca tgggctacga tttagaacta gaccctgaaa aaatccaagc
     6901 catgcatgat aaaatagctg aattcatgcc tgatttggca aactatcctg taaccagaac
     6961 tcgcattgga acgcgcgcct atacttcaac ttattcacca ttctacggaa atattgagga
     7021 tatgcctaat gtctgggtag ctagtggtct aggctccacc ggtctgacaa acggtccaat
     7081 tatcggttgg caaattgcta aagaaattct caatcaagaa acaaactttg accgtacacc
     7141 atattctcca aatagatata ttctcaaaaa ataagatacg atacgtttag attctactaa
     7201 ccctttaaat gtagatacta agactagttg ttatctaacc aataaataca ataagcaacg
     7261 gggaacctcg ttgcttattt ctatctagaa tactaccatt ctagtctcaa actattaaag
     7321 gattatctat agcatgttat cgcaaccata gaaactgatt cacttaaaaa cctaattcgt
     7381 tgaaaatata gtcttttagt ttacttttag tactaggcaa cgagccgcag gcataactgg
     7441 agttaggtaa ggcgagttaa cgaagtaata aaagaaaaac taaatgacga tacttctttt
     7501 acttacgtct tttctttaac aaacctaaag cactaccaag aacaagagtc aatagaccga
     7561 ctcccataat ctgtcttgaa gtaggtgtac ttactttcga agattgttct acgcctaaaa
     7621 tttgttttgc acctgcttga gattgaactc ccatattctt accagattca atagtattat
     7681 ttgaggtttg tgagatattt tctacgagca ctacaggctc agaagcagat gaaaccgttt
     7741 caactaactc tgtctccttg tgttccataa caatttctgc tttagacacc tgacgctctg
     7801 tattattaga ctctaataac acagtattct gatagctcat acctatctca gtactatttg
     7861 agtttgaaga ctgagcaatg gtggcttcta gcaatggaga ccctgcttct gctaagcttg
     7921 attctagcaa ttctttctta ataacttttg attgaacaga gattagcccg tcagtatttg
     7981 tttttctaac ctctcttttc gataatgaag aaacttctac ttctgcattc gtgtccctct
     8041 tagaaccgat aagggagata gctactggtt caatcactgg attatccaga ttaactgatg
     8101 aaacagcagt tagagaggca tctgcatgaa tttcagaatt gtcgacagat gcagcttcta
     8161 cattagttga tttgctcgag gttggttctg caatttctgt atttccagtc tgaatggagt
     8221 ctttttcaag cggagtggct acagattcag ttgtggaacc atctgcatat gccgaggagc
     8281 cacagggagt aactgaagag gtagctgcat gaatttcaga attgtcgaca agttcagctt
     8341 caacactagt tgattcgctc gaggttggtt ctgcaatctc tgtctccccg gcttgaatgg
     8401 agtctttttc aagtggagta gctacagatt ca
//
LOCUS       DBNFCK010000082         8353 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.082, whole genome shotgun sequence.
ACCESSION   DBNFCK010000082 DBNFCK010000000
VERSION     DBNFCK010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8353
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.082"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..1164
                     /locus_tag="AC1PIW_001421"
     CDS             <1..1164
                     /locus_tag="AC1PIW_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HIP6606350.1"
                     /translation="IQDNEKFIQQYLDYLKELGADAIAESQKVLQDLNKYTDSDIMKS
                     QIIELLDLTILPVSEPHSSEEIVEKIKGAIDEIRSALIIDAKKLKENEFYGHYTTIDT
                     LVNFLFKPETEENKNCPTYLRLSNANQLNDPMEGKALLAILGLDAHMEQYYKPTNVFL
                     SSLTVVKDSLPMWKEYAEESRGAFLEYDQDYLETIVKHDVIEFAKVHYITTDEKQNIT
                     GDKLVDQKLIYIQDTIKYLEDEGATDEIREIIDYIAKISYLFKVSDYQYESEYRILVN
                     LDDTHFTNKFTDKFTDKLNSGIKDEATRRKLDIGIGLLENKNVNYLNSRKYIHIEKTE
                     QGKCNLYAYINVVPLKYSKIMLGPKVENVDYIAPYIHYIQPDIIVETSSIPYR"
     gene            1207..1305
                     /locus_tag="AC1PIW_001422"
                     /pseudo
     CDS             1207..1305
                     /locus_tag="AC1PIW_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261961.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
     gene            1478..7258
                     /locus_tag="AC1PIW_001423"
     CDS             1478..7258
                     /locus_tag="AC1PIW_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZmpA/ZmpB/ZmpC family metallo-endopeptidase"
                     /protein_id="HIP6606351.1"
                     /translation="MKRSLGEKRQRFGFRKMSVGLVSAAVTSLFFVSSVATAPTASAQ
                     SINYSYVTEQELTDGEKELIIRDLPGLAQETDVNYYLIYRPATGTTSTPSTSTSQVLP
                     NTGSVETELLVAGGVSLLLLAVRFGKKGKKELAGVILLTATGASFFGPTSSALTSQIL
                     ARYNHAIEISAGQALPAPAEIDGYVYVGYLKDSKAIEQTTSEEEKTAEFPASEGIRTE
                     TIVNKTEAIPFEIQTVENPQLSAGTERVVQEGQDGERIVTIKQVHSKGQIISEEEISS
                     TVTKTAVPKIVEVGTKQATDDIVTEVPDTEPSYENSTNSLTEETVTHTEVFAFDVQEI
                     YDVNLAEGSREVEQKGQDGVRTIETRNYYADGVLIKSEQVSDVVTKEPVTEVVRVGTK
                     TTDVIGSETIVTTEELPFETTVTETEELYVGEEKFITEGKVGSKEVTTTYQTINGVSQ
                     PNPTVTEKVLEEPTTKEVLKGTKPIEGTEIETNQVEITFETEYVDDPTLLEGKTKVVT
                     AGVNGSKTVTTTYQTIKGVRQENSTVTEEITKQPVKQVIARGTKVEKVPQVIITDLVE
                     NDDAKSATISYKLTDETANFQRAVALLYDNTGALVQEQTITDPNGQLTLENLDFYTDY
                     TVKTKIFYTMAEQEQSSEQEAILESMRKFDLVYKKIEIKDIDAVTVYRRKNGSYIGQE
                     FLEELPASTDELFIKVTSDRFKEVYLPVSSIEETTLNGKAVFKLVSSFDELVQDKDAQ
                     YVANREFYIPKMATDANTYTSFKALLDAMKANPSGTFKLGAHLDASEVPVGDVASYVT
                     NFSGTLDGLNDGYAFSISNLKAPLFFNLGGKVQNLDIKNASLNTSSKNPLATIAINAN
                     GATITNVAVEASIKGPQNVSGLVHSATNTTIKGVSFKGSIEVTGTNASLTGGILGNGT
                     MASVGNAKVDATITLPGTENQVAGGIVGRTMLVYDVPGSVYNSYAAGSIVTTESAAIV
                     GGIAGANQVTGAYAPHSGNVNNVVSDMTGTKSIIGQPANPTGKIKDGFTTTSDSLTNV
                     TVITDEEAQAKVEAMAIQATIDDSVPLNPNHYSVDYLTLDKAQADHETAYYNMEKILP
                     FYNKELLVYYGNKIATDDKLNKVRLLDVVPMKDNAVVADVYAEKANINKIMLHYADGT
                     VDYKTVSYLEDFKNNHVVEYTISGTDLIYTPESFLNDRSALVNDLVSSLSSVVLDSDA
                     MKAVINYPTTLNADTQTGTAKDFYFGESYDQVMTNLESNVRKILVASLNGQGQASEDY
                     IKEKIINNKAAFVLGLTYLNRWYDINFGEMNTKDLTIFEPDFFGNDAASALDMILAIG
                     NGGYDVLRAHNNVTTFASIIGKQNNQTKLFDMLEDYRQLFLPEMTNNEWFKQTTKAYV
                     VEGKSLIPEVAAKQETTDTYSKYNVGAYSKIVNDTVSNPTWKYNHMLLPLLTLPQENI
                     FIITNMNTIAIGSYEHYVDDYSTVENRDKVRQMVDLAAERQRDNADFWYKILDETNRD
                     KLFRSVLNNEGYVMYGKDGTKSYRNLTADVDAIQDFYGPINKWYREHPSIKTAFADGS
                     ETYYITYDMLTDYGTALYTHEMVHNQDGDIYLKGYGRRIGQGMEVYAQGLLQNVFNVT
                     EMNLGFNAVYNSDDANRVHVGDPVARFNSEADFNEYYHNQFDVLYLLDYLEGTNILAQ
                     SDANKKAWLRKIENYYVQNNGVDTHAGNSARALTDAEVASLKSFNDLIDQSIIVQRQY
                     VNNPTNTSKKWDRNSYVSVPMFAANFSALSNSNGSPGDIMFRRMAFELIAAKGYTDGF
                     VPYASGQLSDLAMEKGSIIYDTWNKKNTGLITDDHVLEYVFQGQYTSWAEFKKAMFTE
                     RLEKAATGQLKPFTMQYELGVADSTKEVTITSFEQLQNLMKEAMEADIQANSLNLNNS
                     RVHALKVKVYQALMNSTNDFRTSIFN"
     gene            7336..7839
                     /locus_tag="AC1PIW_001424"
     CDS             7336..7839
                     /locus_tag="AC1PIW_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HIP6606352.1"
                     /translation="MMIKPLETKSEMLGKAYVHWKSWQEAYADLLPQEFLKNTYTLER
                     CQDWAVRYPQNILIAVMDDKVVGFACYGSSSQEDLQGVGELYALYVLADYYNQKIGYQ
                     LMQAALEKLQSYETVILWVLEGNARAIAFYEKVGFRFDGVKKTVNLGAERTEYRMIFK
                     QKERENG"
     gene            7832..8299
                     /locus_tag="AC1PIW_001425"
     CDS             7832..8299
                     /locus_tag="AC1PIW_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HIP6606353.1"
                     /translation="MARKEMVTLTNMCLIEDKEGKVVVQIRDPKRYRWSGVAFPGGHI
                     EEGENFHDSVVREVQEETGLTVTDARLVGLKHWPDKEGHRYIVFLYKATEFTGTIRST
                     EEGEVRWVEKSELPQMDLAYDLLEILKVMDEPDLSEFFYTRKNDDGEWDKNFR"
BASE COUNT         2719 a         1522 c         1829 g         2283 t
ORIGIN      
        1 atacaggata atgagaagtt tattcaacag taccttgact atctaaagga attgggggca
       61 gatgcaattg cagaatctca gaaagttcta caagacttga ataagtatac ggattctgac
      121 attatgaaaa gtcagataat agagttacta gatttaacta tcttgcctgt tagcgaacct
      181 cattcaagcg aggaaatcgt tgagaaaata aagggagcta ttgatgaaat tcgttctgca
      241 ttgataattg atgcaaagaa attaaaagag aatgaatttt atggccacta tactacaata
      301 gacaccttgg ttaatttctt atttaaacca gagacggaag aaaataaaaa ttgtccaacc
      361 tatttacgtt tgtcaaatgc taatcaattg aatgatccga tggaaggaaa agccttactc
      421 gccatccttg gtttagatgc ccatatggaa cagtattata aaccaaccaa tgtgtttctt
      481 tcatccttga cagttgttaa agatagttta cctatgtgga aagaatatgc tgaggagtct
      541 aggggagcat ttctggaata tgatcaagat tacttggaaa ctattgtcaa gcatgatgtt
      601 attgaatttg ctaaagttca ttatattact acagatgaaa agcaaaatat tactggtgat
      661 aaactagttg atcaaaaact aatatatatt caagatacaa ttaagtattt agaagatgag
      721 ggagcaaccg atgaaattcg agaaatcatt gattatattg ctaagatatc ctatcttttt
      781 aaggtttctg attatcaata tgaatctgaa tatcgtatat tggtaaactt agatgatacg
      841 catttcacta acaaattcac tgataaattc actgataaat tgaactctgg aataaaagat
      901 gaagctacac gtaggaaact agatattggc ataggattgt tagaaaataa gaatgtgaat
      961 tatctgaaca gcagaaaata tattcacata gaaaaaacag aacaagggaa atgtaattta
     1021 tatgcgtaca tcaatgttgt cccattaaaa tattcaaaga ttatgttggg gccaaaagtt
     1081 gaaaatgtag actatatagc tccttatatc cattacattc agcctgacat tatcgttgaa
     1141 acttcaagta ttccgtatcg ataacctctt ttgaaaagct acgaagaatt gagttagacc
     1201 ataagtaaga aactggaagg tcagatgcag gaagcggcag aagtgcttga ctttgaactg
     1261 gcagcccaga ttcgtgatat ggttatcgag ttgaagaata tgtagattcg aaagaataaa
     1321 gcgacagggt actgcatcct gtcgtttttt atgaacattg cttagtttgt atcagatttg
     1381 aattgaaaat agttcattga ataagacaaa aaacttgtag gagcatcatt gaataggtat
     1441 aatagtttct atactatttt agggagtaac aacttatatg aaaagatctt taggtgaaaa
     1501 gcgacaacgc tttggtttta gaaaaatgtc agtgggactt gtctcagcgg cagtgacaag
     1561 tctatttttt gtgtcctctg tggcaacagc accgactgct agtgctcaat caatcaatta
     1621 ttcctatgtg acagagcaag aattgacaga tggagaaaag gagttgatta ttcgtgactt
     1681 gcctggctta gctcaggaaa cggatgtaaa ttattacctg atctatcgtc cagctactgg
     1741 cacaacatct actccatcga caagcacgtc tcaagttttg ccaaatactg gctctgtaga
     1801 aaccgaacta ttggtggcgg gtggagtgtc tctcctgtta ttggctgtta gatttggtaa
     1861 aaaaggtaag aaggagttgg cgggtgtcat ccttttgaca gctacgggag caagcttctt
     1921 cggtccaact agctccgcat tgacgagtca aattttggct cgatataacc atgcaatcga
     1981 aatatctgcc ggacaagcgt taccggcccc agctgagatt gatggctacg tttatgtagg
     2041 atatttgaag gatagcaaag ctattgaaca aactacttcg gaagaggaaa aaacagcgga
     2101 attcccagct tcagaaggaa tccgtacaga aactattgtc aacaagacag aggccattcc
     2161 attcgaaatt caaaccgtag aaaatcctca attatctgcg gggacagagc gagtggtaca
     2221 agaaggacag gatggggagc gtattgtaac gattaaacaa gtccattcaa aaggtcaaat
     2281 tatttcggaa gaagagattt cctcgacggt tacaaaaact gctgttccaa agattgttga
     2341 agttggtaca aaacaagcaa ccgatgatat tgttacagaa gtaccagata cagaaccaag
     2401 ttatgaaaac tcgacgaatt ctttaacaga agaaacagtt actcataccg aagtctttgc
     2461 ctttgatgtg caagaaatct atgatgtaaa cttggcagaa ggtagtcgtg aagttgaaca
     2521 gaagggacaa gatggtgttc ggacaatcga gactaggaat tattatgccg atggtgtact
     2581 gattaaatcc gaacaagttt cagatgtagt gacaaaagaa ccagtaacag aagttgtaag
     2641 agtaggcacg aagacaacag atgttattgg atctgaaacc attgtaacaa cagaagagct
     2701 tccgtttgaa acgacagtca cagaaacaga agaactttat gttggggaag aaaaattcat
     2761 tactgaaggt aaggttggtt ctaaggaagt taccactact tatcagacaa tcaacggagt
     2821 cagtcaacca aacccaacag tgactgagaa agtccttgaa gagccaacaa cgaaagaggt
     2881 tctcaaagga acaaaaccaa tagaaggtac ggaaatcgaa actaatcaag ttgagattac
     2941 ctttgaaaca gagtatgttg atgatccaac actgctagaa ggtaagacca aagttgtaac
     3001 ggcgggtgta aatggctcta agacagttac aacaacttac cagactatca agggtgttcg
     3061 tcaggaaaat tctactgtaa cagaagaaat taccaaacaa cctgttaagc aagtcattgc
     3121 gcgtggtaca aaggttgaaa aagtaccaca agtgattatc acagatttgg tggaaaatga
     3181 cgatgccaag tcagcgacaa tcagctacaa gttgacagac gaaaccgcta atttccaacg
     3241 agctgttgct ctcctctatg ataatacagg tgccttggtt caagaacaga ccattacaga
     3301 tccaaatggc cagttgacac ttgaaaatct tgatttctac acagattaca ctgttaagac
     3361 taagattttc tacacaatgg cagagcagga acaaagttcg gaacaagaag ctattcttga
     3421 aagcatgcgc aaatttgatt tggtctacaa gaagattgaa atcaaggata ttgatgcggt
     3481 gactgtttac cgtcgtaaga atggtagcta catcggtcaa gaattcttgg aagaactgcc
     3541 agcatccaca gatgaattgt ttatcaaggt gacttcagat cgcttcaagg aggtatacct
     3601 acctgtgtca agtattgaag aaactacctt gaatggcaag gcagtcttca aactagtttc
     3661 atcctttgat gaattggtac aggataagga tgcccaatac gttgccaatc gtgaattcta
     3721 cattcctaag atggcgactg atgcaaatac ctacacatcc ttcaaagctt tgctcgacgc
     3781 tatgaaggca aatccaagtg gtacctttaa actaggagcg catttggacg caagtgaagt
     3841 tcctgttgga gatgttgcat cgtatgtgac caactttagc ggtaccttgg atggcttaaa
     3901 tgacggctac gccttttcta tcagcaactt gaaagcacca ttgttcttta accttggtgg
     3961 taaagttcaa aacttggaca tcaaaaatgc aagcctgaat acaagctcta agaacccact
     4021 tgcgacaatt gcgattaatg ccaatggagc aactattaca aacgtagcag tagaagcatc
     4081 tatcaaagga ccgcaaaacg tatctggttt agttcattct gcaacaaata ctactattaa
     4141 aggtgtatcc ttcaagggta gtatcgaagt aaccggaacc aatgcaagtc taactggtgg
     4201 tatcttgggt aacggtacga tggcaagtgt cggaaatgcc aaggttgatg cgaccataac
     4261 cctcccaggt acagagaatc aagtagctgg tggtatcgtc ggtcgtacaa tgcttgtcta
     4321 cgatgtacca ggaagtgtct ataattctta tgcagctggt tccattgtga cgactgaatc
     4381 ggcagccatt gtcggcggaa ttgctggtgc caaccaagta acaggtgcct atgctccaca
     4441 tagcggtaat gtcaacaacg ttgtcagcga tatgactggt acgaaaagca ttatcggtca
     4501 acccgcaaat ccaactggta agattaaaga cggtttcact acaacatctg atagcttgac
     4561 caacgtcact gttatcactg atgaagaagc acaagcaaaa gtagaagcta tggctattca
     4621 agcgacaatt gatgattctg taccgcttaa tcccaatcat tattcagtag actacttgac
     4681 cttggataag gcacaggctg accatgaaac tgcctactac aacatggaga aaatcttgcc
     4741 attctacaac aaggaacttc ttgtctatta tggaaacaag attgcgacag acgataagct
     4801 caacaaggtc cgtttgctgg atgttgtacc aatgaaggac aatgctgtag ttgctgacgt
     4861 ctacgctgag aaggctaata tcaataagat tatgcttcac tatgctgatg gcactgtgga
     4921 ctacaagaca gtttcttatc tagaagactt caagaataat catgtggtcg agtataccat
     4981 ttcaggtacg gacttgattt acacgccaga atcattcttg aatgacagaa gtgcccttgt
     5041 caatgacttg gtaagcagcc tttcttctgt tgtcctcgac tcggatgcca tgaaggctgt
     5101 tatcaattat ccaacgacct tgaatgccga tactcagact ggtacagcaa aagatttcta
     5161 ctttggtgaa agctatgacc aagtgatgac caacctagaa agcaatgttc gtaagatttt
     5221 agtggctagt ctaaatggtc aaggtcaggc ttctgaagac tacatcaaag agaaaatcat
     5281 caacaacaaa gcagcctttg tgctcggttt gacttacttg aaccgttggt atgacatcaa
     5341 cttcggagag atgaatacca aagacttgac catctttgaa cctgacttct tcggaaatga
     5401 tgcagcatct gccctagata tgattcttgc aattggtaat ggtggctatg atgttcttcg
     5461 tgcccacaat aacgtgacca cttttgcatc catcattggt aaacaaaata atcagaccaa
     5521 gcttttcgat atgctggaag attatcgtca gctcttcttg ccagaaatga ccaacaatga
     5581 atggttcaag caaacaacca aggcttatgt tgttgaaggt aaatccttga ttccagaagt
     5641 agctgctaag caggaaacga cagatacata tagcaagtac aatgttggag cttatagcaa
     5701 gattgtgaat gatactgtat ccaatccaac ttggaagtac aaccacatgc tcttgccact
     5761 cttgactttg ccacaagaaa atatctttat catcacaaac atgaatacca tcgcgattgg
     5821 ttcatatgag cactatgtag atgattattc aacagttgaa aatcgtgata aggttcgtca
     5881 gatggttgat ttggcggcag aacgtcagcg tgataatgca gacttctggt ataagatttt
     5941 ggatgaaacc aaccgtgaca agctattcag atctgtcctc aataacgaag gttatgtgat
     6001 gtatggtaaa gatggtacga agtcttatcg caacttgaca gctgatgttg atgctatcca
     6061 agatttctac ggtccgatca ataagtggta tcgtgagcat ccgtctatta agactgcctt
     6121 tgcggatgga agtgagacct actatattac ttacgatatg ttgacggatt acggtacagc
     6181 tctttatacc cacgaaatgg ttcacaacca agatggtgat atttatctga aaggctatgg
     6241 ccgccgtatt ggtcaaggta tggaagttta cgcccaaggt cttctacaaa atgtcttcaa
     6301 tgttactgag atgaaccttg gattcaacgc tgtctataat agcgatgatg ccaaccgtgt
     6361 ccatgtcgga gacccagttg cacgtttcaa tagcgaagct gatttcaatg agtattacca
     6421 taaccaattt gatgtacttt acttgctgga ttacctagaa ggtacaaaca tcttggctca
     6481 atcagatgct aacaagaaag cttggttgcg taagattgaa aactactatg tccaaaataa
     6541 tggtgttgat acccatgctg gaaattctgc gcgtgcattg acagatgccg aagtggcaag
     6601 cttgaagtcc ttcaatgatt tgattgatca gagcattatt gttcaacgcc aatatgtcaa
     6661 caatccaact aacacaagca agaagtggga tagaaacagt tatgtcagcg ttccaatgtt
     6721 tgcagctaac ttctcagccc tttcaaactc aaacggttct ccaggcgaca tcatgttccg
     6781 ccgtatggcc tttgaattaa ttgctgccaa agggtacaca gatggttttg taccgtatgc
     6841 ttctggccag ttgtctgatc tagctatgga gaaaggctca atcatctatg atacttggaa
     6901 caagaagaat actggtttga ttacggatga ccatgtctta gaatatgtct tccaaggtca
     6961 atatacttca tgggctgagt ttaagaaagc catgtttact gagcgtttgg aaaaagcagc
     7021 gacaggccaa ctcaagccgt ttaccatgca gtatgaactt ggtgttgctg attcaaccaa
     7081 ggaagtaact atcacaagct ttgagcagtt gcaaaacttg atgaaagagg ccatggaagc
     7141 agacatccaa gcgaacagcc tcaacttgaa caacagtcgt gttcatgcac tcaaagtgaa
     7201 agtttatcaa gccttgatga actctacaaa tgacttcaga acatcgattt ttaattaaat
     7261 aatcattaac aagttggtct tgtgccaact tgttttttaa tgtatactag aggaaaaaat
     7321 tcggaggtcc tttagatgat gattaaacca ctggaaacga aaagtgaaat gctaggcaag
     7381 gcctatgttc attggaaatc atggcaggag gcctatgctg acttgttacc tcaggaattt
     7441 ttaaagaata cttacacttt agaacgctgt caagactggg cagttcggta tcctcagaat
     7501 atcttgatag ctgtgatgga tgataaggtc gttggctttg cctgttacgg ctcatctagt
     7561 caggaggatt tgcaaggggt aggggagctc tacgccctct atgtcttggc ggactattat
     7621 aatcagaaga taggctacca gctcatgcag gcagctttag aaaaattgca gagctacgaa
     7681 accgttattc tctgggtatt ggaggggaat gcgcgtgcca ttgccttcta tgaaaaagtg
     7741 ggcttccgat ttgatggtgt caaaaaaacg gtcaatttag gtgcagaacg tacagaatat
     7801 agaatgattt ttaaacagaa agagagagaa aatggctaga aaagaaatgg tcactttgac
     7861 taacatgtgt ctaatcgaag ataaggaagg gaaggtcgtt gtacagattc gagatcccaa
     7921 gcgttatcgt tggtctggtg ttgcctttcc aggtggacat attgaagaag gcgagaattt
     7981 ccatgattcg gttgttagag aagtgcaaga agaaacaggt ttaacagtca ctgatgctcg
     8041 cttggttggt cttaaacatt ggccggataa ggaagggcat cgttacattg tatttctcta
     8101 taaggcaaca gagtttacag gaactatccg atcaactgaa gaaggcgagg tgcgttgggt
     8161 tgagaagtcg gaactgcctc agatggattt ggcctatgac ttactagaaa tcctgaaagt
     8221 catggatgag ccagatttgt ccgagttctt ttatactaga aaaaatgacg atggagagtg
     8281 ggacaagaat tttaggtaga agctaggaat agcttctttt ttatgttgtt tgaataaaaa
     8341 tacaacaaat gag
//
LOCUS       DBNFCK010000083         8192 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.083, whole genome shotgun sequence.
ACCESSION   DBNFCK010000083 DBNFCK010000000
VERSION     DBNFCK010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8192
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.083"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..2255)
                     /locus_tag="AC1PIW_001426"
     CDS             complement(<1..2255)
                     /locus_tag="AC1PIW_001426"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922092.1"
                     /note="This family includes IgM or IgG-cleaving cysteine
                     proteases; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IdeS/Mac family cysteine endopeptidase"
                     /protein_id="HIP6606354.1"
                     /translation="MNIQERFSLRKSAVGLVSVSLLCAIYTSTVAADTVVTGVNEIIE
                     ESQVKDEVSIESEKNESLDGSNIEIVEEIADNIPSPVIAEGEVAVEMKVDRGTENVVS
                     RNDTEVTTSEQNQIEVTETKEILNQTSYQTESGEQRQIIWAHGITPPAMEQSGGFVKE
                     KYGDYLNYTAPFEAGKGYYDTNKSLNASFIDLNLCFAAVSSNMVHWWLEQNSSYVERY
                     LKEKKGTVNVEENYAITDLRRYINSFQNQQNSRVFDMFKTYYGYRTNGFVSDALVDLF
                     INGYKPKAQGGVNLEDSQLVPDSRGGFFYDVFKEKKLTNRIFSGSYERFGEDVRTVLE
                     SKGLLGLTYRTLGYATHIVTVWGAEYDNQGKIKAVYITDSDDQQEQIGLKRMGITRDA
                     SGNPRLNNHMKNNSAGALLDYVHTIRLGQDLWEEYFNPLAKAKETASQTLADTKKALD
                     LSIQGQSELPESMRLIYLEKLNNLYNQGILSIQKAESSEMLSGALENGLNSLKSLDFP
                     ISEVGNALAPDLPVGDRSTVSDVDSLSSQETSSTNLEADTENAGIIADGTNQLHFPVE
                     AQTTSSVEAEGDNVFEQEADTLPIIIENKDEFGSELSRNMQTSETDSLVVAVEEDVKN
                     DEVAQVEELLESEKVENQSSELLSDTLIVESANDKEEDRVEAVVSEQPDSIPHQNVEI
                     SLVEPTNVETETVVTPINDAATPHGSPTYIDNSVTESVATPLEKDSIQAGETEIAEPT
                     SSESTNVETETV"
     gene            complement(2416..3921)
                     /gene="pyk"
                     /locus_tag="AC1PIW_001427"
     CDS             complement(2416..3921)
                     /gene="pyk"
                     /locus_tag="AC1PIW_001427"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIP6606355.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGDDGYWGEKLDVESSAQKIAA
                     LITEGANVFRFNFSHGDHQEQGDRMATVRRAEEIAGKKVGFLLDTKGPEIRTELFEDD
                     AKEYAYTTGDKLRVATKQGIKSTKEVIALNVAGGLDVFDDVEVGKQILVDDGKLGLTV
                     VEKDAVNREFVVLVENDGVIAKQKGVNIPYTKIPFPALAERDNADIRFGLEQGLNFIA
                     ISFVRTAKDVEVVRNICKETGNDHVQLFAKIENQQGIDNIDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIITKVNAAGKAVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPVESVRTMATIAKNAQTLLNEYGRLNSDQFNRASKTEVIASAVK
                     DACHSMDIKLVVTVTETGYSARSISKYRPDADILAVTFTEKVQKSLMLNWGVIPIVTN
                     RPDSTDDMFDLAERIAKEQGLVEAGDDIIIVAGVPVGVAGSTNTMRVRTVK"
     gene            complement(3988..4998)
                     /gene="pfkA"
                     /locus_tag="AC1PIW_001428"
     CDS             complement(3988..4998)
                     /gene="pfkA"
                     /locus_tag="AC1PIW_001428"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIP6606356.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVYGINEGYAGM
                     VAGDIHELSARSVGDIISRGGTFLCSARYPEFAKLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGVPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVVEVMGRHAGDIALWAGIASGADVIVVPEEDFNINDVVDRIKAGYDKGKKHSII
                     VLAEGVMPAAQFAEELKAAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGRGGLAVGIRNEQMVENPILGTAEEGALFSLTTDGKIVVNNPHKADLELADLNRNL
                     SI"
     gene            complement(5083..8190)
                     /locus_tag="AC1PIW_001429"
     CDS             complement(5083..8190)
                     /locus_tag="AC1PIW_001429"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIP6606357.1"
                     /translation="MAQLDTKTVYTFMDSMVTIESYVGRAKELGYHALGIMDKNSLYA
                     AYSFMESCNKMGLQPIIGCELDWKLQGEEQDITTQLIALTTRGYRNLLKISTAKMTGQ
                     DDFETIRQYLVDIAVVVPYFSGIEHLELGVNYYIGVTVDTPVQDFKQAPLPIHTVRFF
                     EEEQLESLQVLHAIRDNIPLNQVSDVDSRQKLLSADSLTNLFSNRFPQAVDNLRDLVA
                     GISYQLNTDLKLPRFNRDRPAVEELYERSYQGLEEKGLTNSLYKERLEKELSIIHQME
                     FDDYFLIVWDLLRFGRSQGYYMGMGRGSAVGSLVAYALNITGIDPVKHNLLFERFLNA
                     ERYSMPDIDIDIPDIHRADFIRYVRDRYGSTHAAQIVTYSTFGAKQAIRDVFKRFGTP
                     EYELSNITKKISFRDSLTSAYQRNAAFRQIINSKIEYQKAFAIAQQIEGQPRQTSIHA
                     AGIVMSDEELTDTIPLKIGDDMLVTQYDAHAVEANGLLKMDFLGLRNLTFVQKMAEGV
                     RERYQQEIDIAKIDLEDKKTLELFAAGRTKGIFQFEQPGAISLLRRVKPGRFEDIVAT
                     TSLNRPGASDYSDNFVKRKHGQETVDLLDPTIAPILQPTYGIMLYQEQVMQIAQVYAG
                     FTLGKADLLRRAMSKKNAQEMQAMEAEFLQGALKNGHAEENAREIFGMMAKFAGYGFN
                     RSHAYAYSALAFQMAYFKSHYTDVFFDVMLNHSSSAYIEDAMQFDFEVARLTINNIPY
                     HDKFDKSKVYLGLKTLKGLPKDLALWILDHRPYKSVEDFILRLPDNFKKKDLLIPLIQ
                     IGLFDDFDANRKKIIENLDNLFVFAEAIGTFFAEESYSWTDVEDYSDSEKFMLEQELL
                     GVGISPHPLVNICRESKRSFTDLADLAVGNRATVLVQIQAIRLIRTKKTGEQMAFLRV
                     TDTKRTLDVTLFPESYRQYASILEEGGMGYITGRVQERDGQLQLVLEKLEPVSLEKFW
                     ILLENREHDYAVARILDKYRGSIPVVLHYQDSNQTIQAEHHMVMKTPQLEEELSEFVM
                     KTIFR"
BASE COUNT         2363 a         1879 c         1378 g         2572 t
ORIGIN      
        1 acagtttcag tttcgacatt cgttgattcg ctcgaggttg gttctgcaat ctctgtctcc
       61 ccggcttgaa tggagtcttt ttcaagtgga gtagctacag attcagttac ggaattatct
      121 atatacgtcg gggaaccatg aggagtagct gcatcattaa taggagtgac cacagtttca
      181 gtttcgacat tcgttggttc tacaagagag atttctacat tttgatgtgg tattgagtct
      241 ggttgttcag aaacaaccgc ctccactcta tcttcttctt tgtcatttgc actctctacg
      301 attagggtgt ctgacagaag ttccgaactc tgattttcaa ctttttctga ttcaagaagc
      361 tcttcaactt gggctacctc atcatttttc acatcttctt caacagctac tactagcgaa
      421 tccgtttctg acgtttgcat gtttcttgat agttctgaac caaattcatc cttgttttca
      481 ataattattg gtaatgtatc tgcctcttgt tcaaaaacat tatctccctc agcctctact
      541 gaagatgtcg tttgggcctc cactggaaaa tgcaattgat tggtaccatc tgcaataata
      601 cctgcattct ctgtgtctgc ttccaaattt gtggaacttg tttcttgaga tgatagagaa
      661 tcaacatctg aaaccgttga gcgatcacct actggtaaat ctggtgccaa agcatttcca
      721 acttctgaaa taggaaaatc taaactcttt aaactattta aaccattttc caatgcacca
      781 cttagcatct cagaactttc tgccttttga atagatagaa ttccttgatt atagagatta
      841 tttagttttt caagataaat cagtcgcatt gattctggca attcagattg tccttgaata
      901 gacaaatcca acgccttctt tgtatcggct aatgtctgac tagctgtttc tttggctttt
      961 gcaagcggat tgaaatattc ttcccataag tcttgaccca gacggattgt atggacataa
     1021 tccaaaagcg ctccagctga attatttttc atatgattat tcaaacgtgg atttccggaa
     1081 gcatcacgag tgattcccat acgcttcaaa ccaatttgtt cttgttgatc atcagaatct
     1141 gtgatataga cagccttaat cttaccttga ttgtcgtact cagcacccca taccgtcaca
     1201 atatgcgttg cataacctaa tgttctataa gttaagccga gtaatccttt gctttccaaa
     1261 acagttcgaa catcctcacc aaaccgctca taactaccac taaaaattcg atttgtcagt
     1321 tttttctctt tgaaaacgtc gtagaaaaag ccacccctac tatctggtac taactggcta
     1381 tcttccagat tgacaccgcc ctgtgcctta ggtttatatc cgttaataaa caagtcaacc
     1441 aaggcatctg atacaaaacc atttgtacga taaccatagt aagttttgaa catatcaaaa
     1501 actcgactat tttgttgatt ttggaatgaa ttaatatagc gccgtaagtc cgttattgcg
     1561 tagttttctt caacatttac tgtacccttt ttttctttga gatatcgctc aacataggaa
     1621 ctattctgtt ccaaccacca atgtaccatg tttgaagaca cagctgcaaa acaaagatta
     1681 aggtcaataa atgaagcatt cagactctta ttggtatcat agtagccttt tccagcctca
     1741 aatggcgctg tatagtttaa atagtctcca tacttttcct ttacaaaacc accgctttgt
     1801 tccattgcag gaggagtaat tccatgggcc catataattt gtcgttgctc gccactctcc
     1861 gtttgataac tggtctgatt caaaatttct tttgtctcag taacctctat ctgattttgc
     1921 tcgctcgtcg taacttctgt atcatttcta gaaactacat tctcggtccc tctgtcaact
     1981 ttcatctcta ccgctacttc cccttcagcg ataacaggtg atgggatgtt gtctgctatt
     2041 tcctctacaa tttcaatatt agaaccatct agggattcat ttttttctga ttcaatagat
     2101 acctcatcct tgacttgtga ttcttcaatt atttcattca ctcctgtaac aactgtatcg
     2161 gcagcaacag tggatgtata aatagcacat agcaaagaga ctgagaccaa gccaaccgcg
     2221 gattttctca aagaaaatcg ttcttgaatg ttcatctttc ctccgtttat atttatattc
     2281 tttgaacaag ttattcttac tcttatttat tttttatatc aacatcccaa aatcatttag
     2341 aggcaaaaaa tctataacag cacgtattta acttattctc taattactac aaaaaggtcc
     2401 aagcggacct ttttattatt ttactgtacg aacacgcata gtatttgttg aacctgcaac
     2461 acctacaggc acaccagcaa cgataataat gtcatctcct gcttcaacaa gaccttgctc
     2521 tttggcaata cgttcagcca aatcaaacat atcgtcagtt gaatctggac ggtttgttac
     2581 aattggaata acaccccagt taagcatcaa tgatttttgt actttctcag taaatgttac
     2641 tgccaagatg tccgcatctg gacggtattt agagatcgaa cgagctgagt aacctgtttc
     2701 tgtaactgtt acaactagtt tgatgtccat tgagtgacat gcgtctttta ctgcagaagc
     2761 aataacttct gttttagagg cacgattaaa ctgatcagag ttcaaacgac catattcatt
     2821 aagaagagtc tgtgcgtttt tagcgatagt tgccattgta cgaactgatt ctactggata
     2881 tttaccgttt gcagactcgc ctgaaagcat tgttgcatca gtaccgtcga taacagcatt
     2941 gaatacgtca gatacttcag aacgagttgc acgtggtttt tcagtcattg tttccaacat
     3001 gtttgttgct gtgataactg ctttaccagc tgcattcact ttagtgataa tcattttttg
     3061 gtaaactgga accatttcga atggtacttc gatacccatg tcaccacgag caatcatgat
     3121 accatctgct gcttcgatga tttcgtcgat gttgtcgata ccttgttggt tttcgatttt
     3181 cgcaaacaat tgaacgtggt cgttaccagt ttctttacag atattacgaa caacttctac
     3241 gtcttttgca gtacgtacaa atgagatagc aataaagttc aaaccttgct caagaccgaa
     3301 gcggatatct gcattatcac gctctgcaag agctgggaaa ggaatttttg tgtaagggat
     3361 gtttacacct ttttgtttag caattacacc gtcgttttca accaaaacaa caaattcacg
     3421 gtttactgca tctttttcaa caactgtcaa accaagttta ccatcgtcaa caaggatttg
     3481 cttaccaact tcaacatcgt caaagacatc caaaccacct gctacgttca aagcaatcac
     3541 ttcttttgta gatttgatac cttgttttgt agcaacacgc aatttgtcac cagttgtata
     3601 tgcgtattct tttgcatcat cttcaaacaa ttctgtacgg atttctggac cttttgtatc
     3661 caacaagaaa cctacttttt taccagcgat ttcttctgca cgacgaactg ttgccatacg
     3721 atcaccttgc tcttggtggt caccgtgtga gaagttgaaa cggaaaacat tggcaccttc
     3781 tgtaatcaaa gcagcgattt tctgtgctga tgactcaaca tctaattttt caccccagta
     3841 accatcgtca ccgaattttt taccaccgcg gatttctacc gctggaccaa gggttgcaac
     3901 aattttaaca cgtttgttca tcttaacaaa actcctttta tttcaatggt catatgaccc
     3961 ttaattaaca cttttaaaca atgataccta gattgacaag ttacggttca aatcagccaa
     4021 ctccaaatct gctttatgcg gattattaac tacaatcttg ccatcagtag tcaagctaaa
     4081 caaggcacct tcttctgcag ttccaagaat tggattttca accatttgct cgttacggat
     4141 acctaccgca agacctccac ggccttcttt aagcaattta acggcatgag cacccatacg
     4201 agaagcgagt acacgatcac gcgctgttgg agaaccacca cgttggatat gaccaagttc
     4261 tgtcacacga agatcacttg tatcgcctgc tgctttcaat tcttcagcaa actgtgccgc
     4321 tggcataaca ccttcggcca agacaatgat gctatgtttc ttacctttat cgtagcccgc
     4381 tttaatgcga tcaacaacat cgttgatatt gaagtcttct tctggaacaa cgataacatc
     4441 tgcaccagaa gcaataccag cccaaagagc gatgtctccc gcatgacgtc ccattacttc
     4501 aacaacgaaa gtacgacggt gacttgatga agtatcgcgg attttatcaa tcgcatccat
     4561 agcagttgtt acagctgtat caaaaccgat tgtaaaatct gtacccacga tgtcgttatc
     4621 aattgtacca ggtacaccaa tagctgggaa accatgctcc gtcaagcgca ttgcaccgtg
     4681 gtaagaacca tcaccaccga taacaactac accttcgata ccatgttttt tcaattgctc
     4741 aatcccttta agttgccctt ctaatttggc aaattctgga taacgagctg aacaaaggaa
     4801 tgttcctcca cgtgaaataa tatcacctac agaacgtgca gacaattcat ggatatcacc
     4861 cgcaaccata cctgcgtaac cttcgttgat tccataaact tccattcctt ctgaaattgc
     4921 ttggcgaaca actgcacgaa tagcagcgtt cataccaggg gcatcaccac cactggtcaa
     4981 aacagcaata cgtttcattc gttaatgctc ctttaacttt tcattttaac tgccctatta
     5041 taacatactt agcctaaact tgcttgactt tttgctattt ttttatcgaa aaatcgtttt
     5101 cataacaaat tcacttaact cttcttctaa ttgaggcgtt ttcataacca tgtggtgctc
     5161 agcctgaatt gtctgattac tatcttgata atgaagtact actggaatgc ttcctctata
     5221 tttatccaaa atacgagcaa cagcataatc gtgttctcga ttttccaaaa gaatccaaaa
     5281 tttctctaaa ctgacaggct ctaatttttc tagaactaat tgcaactgtc catcccgctc
     5341 ttgcactcta cctgtgatgt accccattcc cccttcttcc aaaatagaag cgtattggcg
     5401 ataagattcg gggaaaagag tcacgtcaag cgtcctcttg gtatctgtca ctcgaagaaa
     5461 tgccatctgc tccccagtct ttttggtacg aatcaatcga atagcttgaa tctgcacaag
     5521 aacggtcgct cgattgccaa ccgctaaatc agccaaatct gtaaaactac gcttagactc
     5581 tcgacaaata ttgactaaag gatgcggact aataccaaca cctagcaatt cttgctctag
     5641 cataaatttt tcactgtcgc tgtaatcttc tacatctgtc caactataac tctcctctgc
     5701 aaaaaatgta ccaattgcct ctgcaaagac aaagagattg tctagatttt caataatctt
     5761 ttttcgattg gcgtcaaaat catcaaataa accaatctga ataagaggga ttagtaaatc
     5821 ctttttcttg aaattatctg gcaagcgcaa aataaaatct tctacagact tatagggacg
     5881 atgatccaat atccataaag ctaaatcttt aggcaaacct ttcagtgttt ttaatcctag
     5941 gtaaacctta cttttatcaa acttatcgtg ataaggaata ttgttaatcg tgagtcgagc
     6001 tacctcaaag tcaaactgca tcgcatcttc aatataagca ctgctagaat gattgagcat
     6061 gacatcaaaa aagacatccg tatagtgact cttgaaatag gccatttgaa aggctaaggc
     6121 tgaataagca taggcgtggc tacggttaaa accataacca gcgaacttag ccatcatgcc
     6181 aaatatttcc cttgcattct cttcagcatg tccgtttttc agtgctccct gtaaaaattc
     6241 cgcttccatt gcctgcattt cctgcgcatt ctttttagac atcgccctgc ggagtaaatc
     6301 cgccttgcca agcgtaaagc ctgcatagac ctgtgcaatc tgcatgacct gttcctgata
     6361 aagcatgata ccataagtcg gttgtaaaat aggagcaatg gttggatcca acaaatcaac
     6421 tgtctcttgt ccatgtttgc gttttacaaa gttatccgaa taatcactag cccccggtct
     6481 attcaaactc gttgttgcaa ctatatcttc aaatcgacca ggtttgaccc ttcgaagcag
     6541 gctaatcgca ccaggctgtt caaattgaaa aattccctta gtccgaccag cagcaaaaag
     6601 ttccaaggtt ttcttgtctt ctaaatctat cttggcaata tcaatttcct gctgataacg
     6661 ttcacgtact ccttctgcca ttttttgcac aaaggtcaga tttcgtaagc caaggaaatc
     6721 cattttcaac agaccattcg cctcaacagc atgtgcatca tattgggtta ccagcatgtc
     6781 atcccctatt ttaagaggga tggtatctgt caactcctca tcactcatca ctatccctgc
     6841 tgcatggata gaagtctgtc ggggttgccc ttcaatttgt tgagcaatcg caaaagcttt
     6901 ttggtactct atcttactat tgataatctg gcgaaaagcg gcatttcgct gataggcaga
     6961 agtaagacta tctctaaatg aaattttctt ggtaatatta cttaactcat attcaggtgt
     7021 tccgaaacgc ttaaatacat cacgaatagc ctgcttagct ccaaatgtcg agtaggttac
     7081 aatttgagca gcatgggttg aaccataacg atctcgtaca taacgaataa aatctgctcg
     7141 atgaatatct ggaatatcaa tatcaatatc gggcattgaa taacgttcag cattgagaaa
     7201 gcgttcaaag agaagattat gcttaacagg atcaatacct gtgatattta aggcgtaggc
     7261 aactaggctt ccaacagctg aaccgcgccc cattcccatg tagtaccctt ggctacgtcc
     7321 aaaacgaagt aggtcccata caatcaagaa ataatcatca aattccattt gatgaataat
     7381 ggataattcc ttttccaagc gttccttata cagtgaatta gtcaagccct tctcttctaa
     7441 tccttgatag gagcgctcgt ataattcttc cactgcaggt ctgtcacgat taaaacgcgg
     7501 caattttaaa tctgtattta attgataaga aatccctgca accaagtcac gtaaattatc
     7561 aactgcttgc ggaaaccgat tagaaaatag attggttaag ctatcagccg ataaaagctt
     7621 ttgccgacta tctacatctg aaacctgatt aagcggaata ttatctcgaa tagcatggag
     7681 aacctgtaga ctttccaatt gttcctcttc aaaaaaacga acagtatgga ttggaagggg
     7741 tgcttgctta aaatcttgaa ccggtgtgtc aactgtcact ccgatataat agtttacacc
     7801 caactcaagg tgctcaatgc cagaaaaata aggaacaacc actgctatgt cgactaaata
     7861 ttgacgaata gtttcaaaat catcttgccc tgtcatctta gcagtcgaaa ttttcaatag
     7921 attgcgataa cctctggttg tcagagcaat caattgagta gtgatgtcct gctcctctcc
     7981 ctgtaatttc cagtccagtt cacaaccaat aattggttga agccccattt tgttacagga
     8041 ttccataaaa ctataggctg cataaagact gtttttatcc attatcccca atgcgtggta
     8101 accaagctct ttagccctgc ctacataact ctctatcgtc accatgctat ccataaaggt
     8161 ataaactgtc tttgtatcta attgtgccag ca
//
LOCUS       DBNFCK010000084         8132 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.084, whole genome shotgun sequence.
ACCESSION   DBNFCK010000084 DBNFCK010000000
VERSION     DBNFCK010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8132
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.084"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            67..450
                     /gene="gloA"
                     /locus_tag="AC1PIW_001430"
                     /gene_synonym="lguL"
     CDS             67..450
                     /gene="gloA"
                     /locus_tag="AC1PIW_001430"
                     /gene_synonym="lguL"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HIP6606358.1"
                     /translation="MASKKMLHACLRVENLEASQKFYEEAFGFKETRRKDYPEHKFTL
                     VYLALEGDDFEIELTYNYDHGPYIVGDGFSHLALSSDDLEGDNAKHKELGYPTTDIKG
                     LPGSPGRYYFVTDPDGYRVEVIRAD"
     gene            742..2139
                     /locus_tag="AC1PIW_001431"
     CDS             742..2139
                     /locus_tag="AC1PIW_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIP6606359.1"
                     /translation="MKKVSFWLGINIALLGIMVSLAVWLFAGLQERQVSQFIEEKQQT
                     ILAKGKGKIQEGNIDTTHVVAALPTDDAGHVLGPVESRMISYVQRRFGHKKPAGKIQK
                     LVFVSSIEGKTNFKNVTAREIQAEHYKVDNLQIKKQDKLPSERVLLTQDNELFTLEDL
                     LPNLSSAASIIVDHLREALLAQGMKETDVEAIVKKFETLDLNAISFSYGDSQLTLQLP
                     DGYGINQLVLPISDLYPVVKSDYLVDADKVGYDEYMAAQVVDKKHLRQVALTFDDGPN
                     PNTTPVVLDLLKKYNAKATFFVVGKAVVGNEAILRRMVAEGHVIANHTWNHPNLVTIS
                     GEQVQREIQDTQAAITEATGIVPTMVRPPYGSVNQAVVNQMGLPSIYWSVNSKDWKSR
                     NPQAILKEIKEQTCPGSIILMHDIHQSTVDSLESVLQYLTGEGYNMVTVTDLLASPLN
                     PQLIYYSQELSGPAQ"
     gene            complement(2206..3288)
                     /locus_tag="AC1PIW_001432"
     CDS             complement(2206..3288)
                     /locus_tag="AC1PIW_001432"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIP6606360.1"
                     /translation="MKSKFPSSWTDQLTLLGFEDFTPIQVQAFEPIANGKSLLAISPT
                     GTGKTLAYLWPSLLALTPKKAQQLLILAPNTELAGQIFEVCKTWSETIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNINTIVLDEFDQLFSDSQYQFV
                     EKIINYVPRDHQLIYMSATAKFDRQKIAEDIESIDLSEQKLDNIQHCYMMVDKRERLE
                     TLRKFANIPDFRALAFFNSLSDLGASEDKLLYNGVNAVSLVSDVNVKFRKVIIERFKN
                     HELNLLLATDMVARGIDIDNLECVLNFEVPFDQEAYTHRSGRTGRMGKEGLVITLVSS
                     PSELKQLKKYASVQEVILKNQELYKI"
     gene            complement(3278..4264)
                     /locus_tag="AC1PIW_001433"
                     /pseudo
     CDS             complement(3278..4264)
                     /locus_tag="AC1PIW_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936219.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
     gene            4347..4976
                     /gene="udk"
                     /locus_tag="AC1PIW_001434"
     CDS             4347..4976
                     /gene="udk"
                     /locus_tag="AC1PIW_001434"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HIP6606361.1"
                     /translation="MTQKPIIIGVTGGSGGGKTSVSRAILDNFPNARISMIEHDSYYK
                     NQSHLTFEERILTNYDHPLAFDTDLMIYHISELLAGRSVDIPIYDYTQHTRSEKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDIRIIRRIKRDMEERGRSLDSIIEQ
                     YTSVVKPMYHQFIEPTKRYADIVIPEGVSNVVAIDLINTKVESILRERG"
     gene            4978..5517
                     /locus_tag="AC1PIW_001435"
     CDS             4978..5517
                     /locus_tag="AC1PIW_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIP6606362.1"
                     /translation="MPKQRILPHIILGIMGASGQMVTGKQITDYVQRDLGEFWQVAHS
                     QVYPELKRMTKEELITCHAVPGNEKEKQYAMTATGRQILDEWLSIPNEETPQQKNLFS
                     IKMFFIREKDDPRIPGLLQSQIELVTKHLKHLDSRKVELFSTKESIQDNYGHYLILTR
                     AIERNRAQLKWLEDTLAEM"
     gene            5605..5874
                     /locus_tag="AC1PIW_001436"
     CDS             5605..5874
                     /locus_tag="AC1PIW_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HIP6606363.1"
                     /translation="MSIKQMVSMALVVWNLLVFITYGMDKGKARKNAYRISEKTLLLM
                     SYFGGGLGAWAGGTHFRHKTQKKYFQLAWAIGVLIDAVIMYWMWK"
     gene            5939..6436
                     /locus_tag="AC1PIW_001437"
     CDS             5939..6436
                     /locus_tag="AC1PIW_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIP6606364.1"
                     /translation="MTKQEKISNYQLLLAQLEALLDGETNALANLSNASALLNQALPN
                     SVFTGFYLYDGSELILGPFQGGVSCVHIALGKGVCGEVAAKRQTILVDDVRLHDNYIS
                     CDATALSEIVVPMVKNDQLLGVLDLDSRLVADYDTIDQDYLEKFVALLVEKTDWNFAM
                     FGEKR"
     gene            6436..8103
                     /gene="dnaX"
                     /locus_tag="AC1PIW_001438"
     CDS             6436..8103
                     /gene="dnaX"
                     /locus_tag="AC1PIW_001438"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HIP6606365.1"
                     /translation="MYQALYRKYRSQTFGEMVGQEVVATTLKQAIEQGKISHAYLFSG
                     PRGTGKTSAAKIFAKAMNCPNQVGGEPCNDCYICQAITEGSLEDVIEIDAASNNGVDE
                     IRDIRDKSTYAPSLATYKVYIIDEVHMLSTGAFNALLKTLEEPTENVVFILATTELHK
                     IPATILSRVQRFEFKSIKVTDIQEHLAAILTKEGLIFDEQALTIIARRAEGGMRDALS
                     ILDQALSLSQDQQVTLEIAEEITGSISLRALDDYVASLRQGDSVTALQHLQTLFDQGK
                     SMSRFATDLLHYLRDVLIVQTGGEDTHLTTVFSENLALEQARIFTMIEMVTRGLADIK
                     SSPQPKIYAEMMTIRLAEDSSPSEAMAELPADLLGQLASLQQQVADLQKQLSQLSSQP
                     SAVKPVSRKPQAPKKYRLDTSKVHAILQEAMENPTLARENLTRLQNAWGEIIESLSGA
                     DRALLVGSQPVAANENHAILAFDSPLNAEQTMKRDNLNTMFGNILSKAAGFSPQILAI
                     AQEDWVSIRAEFSAKAKGQKTQEIEKEASPVPEEFGFLADTVEIRND"
BASE COUNT         2408 a         1511 c         1877 g         2336 t
ORIGIN      
        1 tgaatatcgg atttttcata gagagtatgg tatactggtg gtattaaatg gtaggaggaa
       61 tttattatgg caagtaaaaa aatgttgcac gcttgtttgc gtgttgagaa cttagaagca
      121 tctcaaaaat tttatgaaga agcatttgga tttaaggaaa cccgtcgcaa ggactatcca
      181 gagcacaagt ttaccttggt ttacttagca cttgaaggag atgactttga gattgaattg
      241 acctacaatt atgatcatgg tccctatatc gtaggcgacg gtttctcgca tctagctctt
      301 tcttcagatg atttagaagg ggacaatgcg aaacataagg agctgggcta tccaacgaca
      361 gacatcaagg gattgccagg aagtccaggt cgttattatt ttgtgacaga tccagatggc
      421 taccgtgtgg aagtcattcg tgcagattaa tttagaaaag attgcctagg cagtcttttt
      481 cttttcaaaa aaaaatcaga aaatggcagg aggggggaat taggaagcaa atcaattatt
      541 tatactcaat gaaaatcaaa agtagtctag gaaacgaagc cgaagatgga actggagttc
      601 atcaaggaaa gttgacaacg gataattttg attttcgaag agtattacct aatcttttta
      661 tgagttttta tagaactgtc ttgacaagag cctttaaatg atttagcata gtttatatat
      721 gttttgaatg ggaggttgga gatgaaaaaa gttagttttt ggttgggaat caacatcgcc
      781 ttgctcggca taatggtaag tttggctgta tggttatttg caggacttca agaaagacag
      841 gttagtcagt ttattgagga aaaacaacaa accatcctcg ctaaaggcaa gggcaaaatt
      901 caagagggaa atattgatac gacccatgtt gtggctgcct tacctacaga tgatgcaggt
      961 catgtcttag gacctgtaga aagtcgaatg atttcctatg ttcaaagacg ctttggtcat
     1021 aaaaaaccag caggaaaaat tcaaaaactg gtctttgttt catcaataga gggaaaaaca
     1081 aattttaaaa atgtaacagc tcgtgaaatt caagcggagc actataaagt agacaacttg
     1141 caaattaaaa agcaggataa actcccgtct gagcgtgtgt tattgacgca ggacaatgaa
     1201 ttgtttactc tagaagattt attacctaat ttatcctctg cagcaagtat tattgttgat
     1261 catctaagag aagccttact tgctcaagga atgaaagaga ctgatgtaga agcgattgtc
     1321 aaaaaatttg aaacactaga cttaaatgct atttccttta gttatggaga cagccaatta
     1381 accttacaat taccagatgg ttacggcatc aaccagttgg ttctaccaat ttcagatttg
     1441 tacccagtag tgaagagtga ttatctagta gatgctgata aggttggcta tgatgagtat
     1501 atggctgcac aagttgtgga taagaaacat ttgagacagg tcgctctaac ctttgatgat
     1561 ggacccaatc cgaacacaac accggtagtt ttagatttac tgaaaaaata taatgccaaa
     1621 gcaactttct ttgttgttgg taaggctgta gttgggaatg aagctattct ccgtagaatg
     1681 gtagcagaag ggcacgtcat cgccaaccat acgtggaatc atcccaatct agtcactatt
     1741 tctggggagc aagtacagag agaaattcaa gatacacagg cagctattac tgaagctaca
     1801 ggtattgtac caacaatggt ccgtcctcct tatggttctg ttaaccaggc cgttgtcaat
     1861 cagatgggac ttccatcgat ttattggtcg gtcaattcta aagattggaa gagtcgcaat
     1921 ccccaagcca ttttgaaaga aataaaagaa cagacttgtc caggaagtat tatcctaatg
     1981 catgatattc atcagtcaac agttgatagc ctcgagtctg tattgcaata tttaacaggt
     2041 gagggctaca atatggttac tgtaacagac ttactcgcta gtcctctcaa tcctcaatta
     2101 atctattatt cacaagaatt atccggccca gcccagtaag aatgataaaa ggcttagaat
     2161 catgttagta gaaacactgg ttctaagcct tttgtatatt gttcattata ttttgtagag
     2221 ctcttgattc ttgagaatga cttcttggac ggaggcatac tttttcaatt gttttagttc
     2281 gctaggacta gatactagag tgatgacaag accttccttg cccatgcgtc cagtacgtcc
     2341 agaacgatga gtgtaggctt cttggtcaaa aggaacttca aagttgagga cacattctaa
     2401 gttatcaata tcaataccac gtgcgaccat atctgtagca agtagaagat tgagttcgtg
     2461 attcttgaaa cgttcgatga tgaccttgcg gaattttacg ttgacatcag aaaccaatga
     2521 aactgcattc acaccattat agagtagttt atcttcgctt gcaccaaggt ctgataggct
     2581 gttaaaaaat gcaagagcac gaaaatcagg gatattggca aacttacgta gagtttccaa
     2641 acgttcacgc ttatctacca tcatatagca atgttggata ttatccaatt tctgctcaga
     2701 taaatcaatg ctttcaatgt cttcagcaat tttttgacgg tcaaatttag ctgttgcact
     2761 catgtagatt aactggtggt cacgaggcac atagttgata atcttttcga caaactgata
     2821 ttgagaatcg gagaacagct gatcaaactc atctaggacg atggtgttga tattcatcat
     2881 cttgattttt ttcaatttaa tcaactcgaa aatacgacca ggtgttccaa tcaggatttc
     2941 tggtcctttt tttagtcgct caatctggcg tttttgactg gaaccagaca agaatagttg
     3001 agcagtcaac ccaattgtct ccgaccaagt cttacaaacc tcaaatattt gaccagccag
     3061 ctctgtattg ggtgcgagga tgagcaattg ttgggctttt ttaggagtta gggctaatag
     3121 gctaggccag aggtaggcaa gggtttttcc tgtgcctgtt gggctgatag ctagaaggga
     3181 cttgccgttt gcaataggct caaaggcctg gacttggata ggagtaaagt cctcaaagcc
     3241 aagtagggta agctggtccg tccagctgga gggaaattta gatttcattg ttatccgcct
     3301 taaagattat tcctgcgtct tgacgcatgg tgtaaagtgt ttgatggacg ctttctgcca
     3361 cagccagcca ctggtccaca agcttgcggt tttgttgctt gattgcctgt gcaatttcta
     3421 acacttcttc gagcatagga tgctgggccg cttggattgg taaaacaatt tgctctccgt
     3481 catgttttgt gaaaatagcg gagctgatat gctggcatgc gttgagtgtt agtgttccat
     3541 catttgtata gatttcgctc gctagattgc ttgtaatatt ttttcatgct tgaatgtgga
     3601 cgatatagtt gtcataaatg agttgtcccg aaccgtttaa gtcaatacca aaaggtagct
     3661 gttgtgctac atagcgggcc gttctgggag ttcccaaaat gccgatagcg gcgtagagcg
     3721 tatagacacc aaaatccatg agagcaccac cggaaaaatc agttgaaaag atatttggca
     3781 tttgcccagc taacaattca ggcatcttag aagaatactt ggcataccca aatgaaccac
     3841 ctaaaatcgt tttatccgtc aaaaattcac ggataatggc ttgagcttgt tcgtgatagt
     3901 ttcgagcagc ctcaaagagg aaaacattat tttcttgggc aacgcgacgc aattgttcta
     3961 attcctgagg tgtagaaacc atgggtttct caacaatagc atgtttacca gcagctagaa
     4021 cagcttttgc ctgttcaaag tgcagggcat ttggactggc aatatagaca acatcaatgg
     4081 gagcagaaag aaattttacc cattctgtgt ataaatcgac tccttcatac tgctctgaaa
     4141 aggcagcagc agtttctagt ctacgggaga aaacagcctg tagctgataa tgtccactta
     4201 aatgggctgc ctctataaaa cgatgagaga tgtctgaggt gccaacaata ccaaaattaa
     4261 gcataaattc tcatttcttt tgtgttatcc ttttcattat accatgtttc atggtaaaat
     4321 agtattgaaa atagaaggag atgatgatga ctcagaaacc cattattatt ggcgtaaccg
     4381 gcggatctgg gggtggcaag acaagtgtgt cacgtgccat tctggataat ttcccaaatg
     4441 ctcgtatatc tatgattgaa cacgattctt attataagaa tcagtctcac ctgacctttg
     4501 aagaacggat actgaccaat tatgaccatc cactggcttt tgatacggat ttgatgattt
     4561 atcatatcag tgagctatta gcaggacgtt cagtggatat tccgatttat gattacactc
     4621 agcacacacg ttcagagaag acctatcgtc aggaaccaca agatgtattt attgtggaag
     4681 gtattttagt attggaagac aaacggttgc gggatttaat ggatattaag atattcgttg
     4741 atactgatga cgatattcgt atcattcgcc gcattaagag agacatggaa gagcgtggcc
     4801 gtagcttaga tagtatcatt gagcagtaca cctcggtggt gaaaccaatg tatcaccagt
     4861 ttatcgagcc aaccaagcgc tatgcagata tcgtcattcc tgaaggcgtc agcaacgttg
     4921 tagccatcga cttgattaac accaaggtgg agagtatatt aagagagcgt ggttaagatg
     4981 ccgaagcaaa gaatattacc tcatatcata ttagggatta tgggtgctag cggtcaaatg
     5041 gtaactggca agcagattac cgactatgtt caacgtgatt taggcgaatt ttggcaggtt
     5101 gcccatagcc aagtctatcc agaattaaaa cgtatgacca aggaagaatt gattacttgt
     5161 catgcagtac ctggaaatga aaaggaaaag cagtatgcga tgacggctac tggtcgtcag
     5221 attttagacg aatggctatc cattccaaat gaagaaacgc ctcagcaaaa aaacttgttt
     5281 tcaatcaaga tgttttttat ccgtgaaaag gatgacccac ggattcctgg tttgttgcag
     5341 agtcaaattg aactagtcac caagcactta aaacacctag acagtcgtaa agttgagctg
     5401 ttctcaacca aggaaagtat ccaagacaat tatggacact atttgatttt gacacgggct
     5461 attgaacgca accgtgccca gctcaaatgg ttagaggata cactggcaga aatgtaacga
     5521 ttcggatttc cgaatcgttt ttccttgtgt cctgttttgg attatgttag agtaagaaga
     5581 aatgtgactt aattggagga atgtatgtca atcaaacaga tggttagtat ggcgctagta
     5641 gtttggaatt tacttgtctt cattacctat ggtatggata aagggaaggc aagaaaaaac
     5701 gcctatcgga tttcagaaaa gaccttatta ctcatgtcct attttggtgg cgggctgggt
     5761 gcctgggcag gtggaaccca ttttcggcat aagacacaga aaaagtattt tcaactagcc
     5821 tgggctatag gggtattgat tgatgcagtg attatgtact ggatgtggaa ataggcgcaa
     5881 gcctttttct ttatcgctcg gactataaaa gtcggcttaa ctatgctata atgttcctat
     5941 gacaaaacaa gaaaaaattt caaactatca attattgttg gctcaattgg aagctcttct
     6001 tgatggggaa accaatgctt tagccaatct atcaaatgct tctgctttgc tcaatcaagc
     6061 tctaccaaac tctgtcttta caggatttta cttgtatgat ggtagtgaat tgattttggg
     6121 gccttttcag gggggcgttt cctgtgttca tattgcactt ggaaagggtg tctgcggtga
     6181 ggttgcagct aagcgccaga ctattctcgt agacgatgtt cgtctacatg ataactatat
     6241 ttcctgcgat gcgacagccc tatcagaaat cgttgtgcca atggtgaaaa acgaccagtt
     6301 gctgggcgtt ttggacttgg attcgagatt ggtagctgat tatgatacga ttgaccaaga
     6361 ctatctggaa aagttcgttg ctctcttggt tgagaagacg gactggaatt ttgctatgtt
     6421 tggagaaaaa cgctaatgta ccaagcttta tataggaagt accgaagcca gacctttggg
     6481 gagatggtgg ggcaggaggt ggttgcgacc acactcaaac aggccattga acaagggaaa
     6541 atcagtcatg cctatctctt ttcaggtcct cgtggtacgg gtaagacatc ggcagctaag
     6601 atttttgcca aggccatgaa ctgtcctaat caggttggag gcgagccttg taacgattgt
     6661 tatatctgtc aggccattac ggaaggtagc ctggaagatg tgattgagat tgatgcggct
     6721 tccaacaatg gtgtggatga gattcgtgat attcgtgata aatcgaccta tgcacctagt
     6781 cttgcgacct ataaggtcta tatcatcgac gaggttcaca tgctgtctac tggagccttc
     6841 aacgccctct tgaagacctt ggaagaaccg actgaaaatg tggtctttat tctagcaacg
     6901 acggaattgc acaagattcc tgctaccatt ctgtcaaggg tgcaacgatt tgaatttaag
     6961 tccatcaagg tgacggacat tcaggagcac ttggctgcaa ttttaaccaa ggaagggcta
     7021 atctttgacg aacaggctct gacaatcatt gcgcgtcggg cagaaggagg gatgcgggat
     7081 gccctatcta ttttggatca ggctctcagc cttagtcagg accagcaagt gaccttggaa
     7141 attgctgagg aaattacggg ttctatcagc ttgcgtgcct tggatgacta tgtggcaagt
     7201 ctgcgtcaag gggatagtgt cacagctctc cagcatctac aaaccttgtt tgaccaagga
     7261 aagagtatga gccgttttgc gacagatctt ttgcactatt tgagagatgt ccttatcgtg
     7321 cagacgggtg gagaggatac gcatttaaca acagtttttt cagaaaatct agctcttgag
     7381 caggcacgga tttttaccat gatagagatg gtgaccaggg gtttggcgga catcaagtcc
     7441 agtcctcaac ccaagattta cgctgaaatg atgaccattc gtttggctga agatagttct
     7501 ccttcggagg caatggcaga gctgccagct gatttactgg ggcaactagc cagtctgcaa
     7561 caacaagttg cggatttaca gaaacaacta agtcaattat ctagtcaacc aagtgctgtt
     7621 aagccagttt cacgcaagcc gcaggctccg aagaagtacc gattggatac cagcaaggtt
     7681 catgctattt tgcaggaagc tatggaaaat ccaaccttgg cgcgtgaaaa tttgactcgc
     7741 ttgcagaatg cttggggaga aattatcgag agtctgtcag gtgcagatcg tgccttgttg
     7801 gttggttccc agccagttgc tgccaatgaa aatcatgcga ttctagcctt tgattctccg
     7861 cttaatgctg aacagacgat gaagcgtgac aatctcaata ccatgtttgg aaatattctc
     7921 agcaaggcag ctggtttttc cccacagatt ttagctattg ctcaggaaga ttgggtaagt
     7981 attcgtgctg aattttcggc gaaagcaaaa gggcaaaaga cacaggaaat cgaaaaagaa
     8041 gccagtccag tcccagaaga atttggcttt ctcgcagata cagttgagat tagaaatgat
     8101 tagaaaatat cagtcctaag actgatgaat ag
//
LOCUS       DBNFCK010000085         7965 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.085, whole genome shotgun sequence.
ACCESSION   DBNFCK010000085 DBNFCK010000000
VERSION     DBNFCK010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7965
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.085"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            9..1028
                     /locus_tag="AC1PIW_001439"
     CDS             9..1028
                     /locus_tag="AC1PIW_001439"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIP6606366.1"
                     /translation="MFTPISEKIDINQVREHSKLSPETLAKKQARDRELEAILKGEDN
                     RLLLVIGPCSSDNEEAVLDYAHRLAKLQEEVKDKIFMVMRVYTAKPRTNGDGYKGLMH
                     QPDTDAAPSLINGIKAVRNLHYRVITETGLTTADEMLYPENLPLVDDLVSYIAIGARS
                     VENQQHRFVASGIDVPTGLKNPTSGNLKVMFNGLFAAQKKQSFLFNNTEVETSGNPLA
                     HVILRGAVNENGKYLPNYYYDNLLETIELYDQFGLKNPFIIIDTNHDNSGKNYLEQIR
                     IVRQTLINRDWNESIRNYVRGFMIESYIEDGRQDNPDVYGKSITDPCLGWEKTQELIR
                     EIYNR"
     gene            1038..2063
                     /locus_tag="AC1PIW_001440"
     CDS             1038..2063
                     /locus_tag="AC1PIW_001440"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIP6606367.1"
                     /translation="MGIHKRSTSLDIDKVKELSKLEGEFLAAKNQRDEELKRIIRGED
                     DRLLLVIGPCSSDNEEAVLEYARRLSKLQEEVKDKIFMVMRVYTAKPRTNGEGYKGLV
                     HQPDTSKLPDLINGIAAVRNLHYRVITETGLTTADEMLYSANYPLVEDLVSYHAIGAR
                     SVEDQEHRFVASGVDMPTGMKNPTSGNLTVMFNGIYAAQNKQNFIYRDAEVDTDGNPL
                     AHAILRGANTEKGGYEPNYYYDILLKTIKQYEQFGLKNPFIVIDTNHDNSGKNYLEQI
                     RIVRQTLINRDWNEAIRKYVRGFMIESYLEDGRQDSPEVFGKSITDPCLGWEKTEALI
                     REIHQTV"
     gene            2109..2978
                     /locus_tag="AC1PIW_001441"
     CDS             2109..2978
                     /locus_tag="AC1PIW_001441"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIP6606368.1"
                     /translation="MNIDGYTRLAAVVAKPIKHSISPFIHNLAFKETGVNGVYVAWEI
                     PEEDLAVTLENIKRYDMFGINLSMPYKQAVIPYLDSLTDSARLIGAVNTVIHQDGLLI
                     GHNTDGIGFFKSLEKLRGFQVRNKRLTILGGGGASTAIIAQATLDGAKEITIFCRQQS
                     LERTQASLTPIARATGVPMQILALDDSQLLQEHITNSDLLVNGTSVGMDGYSQPVPST
                     IRFPENLLVADVIYQPFETPLLKLAQSQGNPTINGLGMLLFQAAEAFQSWTGQEMPTD
                     LIWDQLVQKYDIK"
     gene            2991..4058
                     /gene="aroB"
                     /locus_tag="AC1PIW_001442"
     CDS             2991..4058
                     /gene="aroB"
                     /locus_tag="AC1PIW_001442"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIP6606369.1"
                     /translation="MKLTVNLPNTPYDILIQRGSLAQTGAWVKELWKPQKIAIITDDH
                     VGSLYRETVQSSLEQAGFETIVFEFPEGEASKNLDTVNQAYEFLVKNGMTRSDGIIAL
                     GGGVVGDLAGFVASTYMRGIHFLQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFCQP
                     DGVLIDPDTLKTLGKRELIEGMGEVVKYGLIDDVELWETLDQLDGSVESILEHADYII
                     YHSCEVKRKVVVEDELDNGVRLYLNFGHTIGHAIEATAGYGQVMHGEAVAIGMVQISR
                     AAEKKGLMPAGMTAKIIAMCEKFGLPTTHQPWNVDELYASLTHDKKTRGKTIKLVIVP
                     QLGQAAIHQIPMEEMLEFLQV"
     gene            complement(4263..5063)
                     /locus_tag="AC1PIW_001443"
     CDS             complement(4263..5063)
                     /locus_tag="AC1PIW_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6606370.1"
                     /translation="MSTILSIQNIHKTFEAGTVNENHVLRGLNLDVKEGDFISIIGGN
                     GAGKSTFMNSLAGALTVDSGDILLEGKSIKYVSAAKRSKDISRVFQDPKMGTASRLTI
                     EENMAIAYRRGLSRGLGWGVKDSERAIFKEALKELGLGLENRMKVDTQFLSGGQRQAL
                     TLMMASLVKPKVLLLDEHTAALDPKTSDMVMELTKKIVESHQLTALMITHNMENAIEY
                     GNRLVMLHRGQIVVDVEGEEKKNLTVQNLMDLFYKNSGEKLTDDEMIL"
     gene            complement(5073..5966)
                     /locus_tag="AC1PIW_001444"
     CDS             complement(5073..5966)
                     /locus_tag="AC1PIW_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6606371.1"
                     /translation="MDIILSSISQGLLWSVMAIGVYLTFRILDIADMTAEGSYPLGAA
                     VCATGIVNGVNPLLATFMAMVAGMGAGLISGLLHTKLKIPALLTGIVTLTGLYSINLK
                     ILGKANVALLKQETLVTQLQDFGLTKTNAVLVIGLVFVLAVVGLLTLLLNTQIGLAIR
                     STGDNIPMSEANGINVDNMKIYGYMLSNGLIALCGALLTQNNGYADLNSGTGTIVIGL
                     ASVIIAEVILRNLRLGWRLLSVVLGAVVYRLIILAILEIPGMDADLVKLFSAILLATV
                     LYVPELQKKLNIRRPKLNGNA"
     gene            complement(6021..6995)
                     /locus_tag="AC1PIW_001445"
     CDS             complement(6021..6995)
                     /locus_tag="AC1PIW_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIP6606372.1"
                     /translation="MYYKLVKKLATISVASMGLLTLAACSSSSEQASSDVVKVGVLQY
                     MEHESLTAAREGFVAELEANGYKEGEKLVLDYQNAQGDQANLQTISEQLIDGNDIVLA
                     IATPSAQSLATVSTETPIVFTAVTDPLSADLVESIEKPGGLLTGTSDQAPIDKQVELL
                     GQAVPDAKTVGILYTTSERNSEVQVEQAKELLEKAGYKVVVKGITSSNEVQDATTSLM
                     KDVDALFIPTDNTVASTMTMIGELSVEHKVPVIGGSTDMVDEGGLLTYGTNYEALGRQ
                     TAKMAIKIIEGANVSETAVEYPETVSLHVNEEMAQKLGIDTSKLAVSE"
     gene            complement(7019..7963)
                     /locus_tag="AC1PIW_001446"
     CDS             complement(7019..7963)
                     /locus_tag="AC1PIW_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIP6606373.1"
                     /translation="MILSSVALLSAVTLAACSSNSSDSSSVNVGIIQYAEHEALSSAR
                     EGFVEALEEAGYKEGENLTIDLQNAQGDQANLQTMVEKLAGKNDLNFAIATPAAQTML
                     NADSETSSVFTAVTDPVEAGLVESLEKPGGTMTGSIDATDVEGQIEMLLKVVPTAKTV
                     GIFYNSSEVNSEVQAEQAKKALEAKGVKVEVTTVTTTNDVQQAITSLAGKVDAIYLPT
                     DNTVASTASTIGDILKEAKVPAMGSDAAVIDAALFTYGVDYHAIGVQAGELAVKILKG
                     EKPADLAVEKPNTAAITVNEEMAKAVGIDAATIKALEE"
BASE COUNT         2374 a         1833 c         1532 g         2226 t
ORIGIN      
        1 gagatctatt gtttacacca atcagcgaaa aaattgacat caatcaagta cgagaacatt
       61 caaaactttc tccagaaaca cttgctaaaa aacaagctcg tgaccgtgaa cttgaagcca
      121 tcttaaaagg tgaagataat cgtcttctcc tagtaattgg accatgttct tctgacaatg
      181 aagaggctgt cttagattat gctcaccgct tggccaaact ccaagaagag gtcaaagata
      241 aaattttcat ggtcatgcgc gtctatactg ccaagccacg taccaatggt gatggttata
      301 aaggactcat gcaccaacca gatacagatg ctgcaccaag cctcatcaac ggaatcaaag
      361 cagttcgtaa cctccactat cgtgtcatta cggaaacagg tttaactact gctgacgaga
      421 tgctctaccc tgaaaacctg cctttggttg atgacttggt ttcctacatc gctatcggag
      481 ctcgttcggt tgaaaatcag cagcaccgct ttgtagcgtc aggcatcgat gttccaacag
      541 gcttgaaaaa tccaacgtct ggtaacctca aagtcatgtt caacggtctt tttgctgctc
      601 agaagaaaca atccttcctc ttcaacaata ctgaagttga aacatctggc aatccacttg
      661 ctcacgttat tcttcgtggc gcagtcaatg aaaatggtaa atacctacca aactattact
      721 acgacaatct cttggaaacc attgagctct atgaccaatt tggtttgaaa aatccattca
      781 tcatcatcga taccaaccat gacaattctg ggaaaaatta tttggaacaa atccgtatcg
      841 tccgtcaaac cttaatcaac cgtgactgga acgaatccat tcgtaactac gttcgcggat
      901 tcatgattga atcctacatt gaagacggtc gccaagacaa tccagatgtc tatggaaaat
      961 ccatcacaga cccttgctta ggctgggaaa aaacacaaga actcatccga gaaatttaca
     1021 acagatagga agatactatg ggaattcaca aacgtagcac cagtttagac atcgataaag
     1081 taaaggaact ttccaagtta gaaggagaat ttctagctgc aaaaaatcag cgcgatgaag
     1141 aactgaaaag aattatccga ggcgaagatg accgtctgct cttggttatc ggtccttgct
     1201 catcagataa tgaagaggcc gtcctagaat atgcacgtcg tctctctaag cttcaagagg
     1261 aagtcaaaga taaaatcttc atggtcatgc gggtttacac agctaagcca cgtaccaatg
     1321 gggaaggcta taaaggactg gttcatcagc cagatacttc taaattacca gacctcatta
     1381 acggaattgc agccgttcgt aatttgcatt accgtgtcat tactgagact ggactcacaa
     1441 ctgctgatga aatgctctac tctgccaact accctcttgt ggaagacttg gtgtcctacc
     1501 atgctatcgg agcacgttcg gttgaagacc aagaacatag atttgttgca tcaggtgtcg
     1561 acatgccaac tggtatgaaa aacccaactt ccggtaattt gaccgttatg ttcaatggta
     1621 tctacgccgc acaaaacaaa cagaacttta tttaccgtga tgcagaagtt gatacagatg
     1681 gcaaccctct agctcatgct atccttcgtg gtgccaatac tgaaaagggc ggttatgagc
     1741 caaactacta ctatgatatt ctcttgaaaa ccatcaaaca gtatgaacaa tttggtctta
     1801 aaaatccatt tatcgtaatt gataccaacc atgataattc tggaaaaaat tacctggagc
     1861 aaatccgtat cgtccgtcaa accctcatca accgtgattg gaatgaagct attcgcaagt
     1921 acgttcgtgg tttcatgatt gaatcctact tggaagacgg ccgtcaagat agccctgagg
     1981 tattcggcaa atccattacc gatccttgct taggctggga aaaaacggaa gctctgattc
     2041 gtgaaattca tcaaaccgtc taattctttc cttaattttt tatacgataa ataatttgga
     2101 ggtaagctat gaacattgat ggttacactc gtcttgctgc cgttgttgca aagccaatca
     2161 aacactccat ttcccccttc attcataatc tggcttttaa ggaaacaggt gtaaatggtg
     2221 tctatgtcgc ttgggaaatt cccgaggaag atttggctgt aacgcttgaa aatatcaaac
     2281 gatatgatat gtttggtatc aacctgtcca tgccctataa gcaggctgtc atcccttatc
     2341 tcgatagcct gactgactct gctcgcttga ttggcgctgt caataccgtc attcatcagg
     2401 atggcttact aatcggtcat aataccgatg gcattggatt cttcaaaagt ttagaaaaac
     2461 taagaggatt ccaagtacgc aacaagcgac taaccatcct aggtggtggt ggtgcttcta
     2521 ctgctatcat agctcaggct acattggatg gtgcaaagga aattaccatt ttctgccgtc
     2581 aacaaagctt ggaaagaaca caggcgagtc ttacacctat tgctcgagca actggagttc
     2641 ccatgcagat cctagcgctt gatgatagcc aattactaca ggaacatatc accaactccg
     2701 acttactagt caacggaacc agtgtaggca tggatgggta ttctcaacct gttccttcta
     2761 ccattcgctt tcccgaaaat cttttggttg cggatgtgat ttatcaacca ttcgaaacac
     2821 ctttattaaa actagcccaa tcacaaggca atccaactat caacggtttg ggcatgctcc
     2881 ttttccaagc agctgaagcc ttccaatcct ggactggaca ggaaatgccg actgacttga
     2941 tttgggacca attagtccag aaatatgaca tcaaatagag aggaaatctg atgaaactga
     3001 ccgtcaatct tcccaacact ccctacgaca tccttatcca aagaggaagt ttagcacaga
     3061 caggtgcatg ggtcaaagaa ctttggaaac ctcagaaaat cgccattatt acagatgacc
     3121 acgttggctc actctaccgc gaaacagttc agtcaagctt agaacaagct ggttttgaaa
     3181 ccattgtttt tgaatttcca gaaggtgaag cctcaaaaaa tcttgatact gtcaaccagg
     3241 cctacgaatt tctcgtaaaa aatggtatga cacgaagtga tggaatcatt gctctcggcg
     3301 ggggagtcgt tggcgatttg gcaggttttg tcgcttctac ctatatgcgt ggtatccatt
     3361 tcctacaaat tccaaccagc ctaactgccc aagtcgattc ttctatcggg ggaaaaactg
     3421 gcgtgaacac cccatttgcc aaaaatatgg tggggacctt ctgccagcca gacggcgttc
     3481 tcattgaccc tgatacccta aaaacactcg gcaaacgaga attgattgaa ggaatgggcg
     3541 aggttgtcaa atatggctta atagatgatg tggagctctg ggaaacccta gaccaattag
     3601 acggctctgt cgagagcatt ctggagcatg ctgactatat tatctaccac tcctgtgaag
     3661 tcaaacgcaa ggttgttgtc gaagatgaat tagacaacgg tgtcagactc tatctcaact
     3721 tcggtcacac gattggacat gctattgaag cgaccgctgg ctacggccag gtcatgcacg
     3781 gtgaagctgt tgccatcggt atggtacaaa tctcacgcgc agctgagaaa aaaggactga
     3841 tgccagctgg tatgacagca aagattatcg ccatgtgtga aaaattcggc ttgccgacta
     3901 cacaccagcc ttggaatgta gatgaattat acgcctccct aactcacgat aaaaaaacac
     3961 gtggcaagac catcaaactt gtcatcgttc ctcaattggg acaggctgcc atccatcaaa
     4021 ttccaatgga agaaatgcta gaatttctac aagtatagtc atttagttga cttttacgtc
     4081 gaagtaataa aagtcaacta atactcaatg aaaatcaaaa acagactagg cgacgcagat
     4141 gcagatagaa ctgaagttca tcaaattaag tcaacaacgt ctgattttga ttttcgaaga
     4201 gtataaatga cgatataaga aaaaagacta ctgacttgca tgaaatcagt agtcttattt
     4261 ttttataaaa tcatctcatc gtcagttagc ttttcaccgc tgtttttgta gaaaaggtcc
     4321 atcaagtttt gaacagtcaa gttcttcttc tcttctcctt ctacatcgac aacaatctga
     4381 ccacggtgca acatgaccaa acgattacca tattcaatgg cgttttccat attatgcgta
     4441 atcatcagag ctgtcaactg atgactttcc acgattttct tggtcaattc cataaccata
     4501 tcgctggttt ttggatcaag ggccgcagtg tgttcgtcca ggagcagaac ttttggttta
     4561 accagcgaag ccatcatcaa ggtcagagcc tgacgttgac cacctgaaag aaattgcgtg
     4621 tccaccttca tacggttttc tagtcccaga ccgagctctt tcaaggcttc cttaaaaata
     4681 gcacgttcac tatctttgac cccccagcca agaccacgtg acaatccacg acgataggca
     4741 atagccatat tttcttcaat ggtcaagcga gaagctgttc ccatcttagg atcttggaaa
     4801 acacggctga tgtccttaga gcgtttggca gctgagacat acttaatgga tttaccttca
     4861 agcaaaatat cgcccgaatc aaccgtcaag gcacctgcaa gtgagttcat gaaagttgat
     4921 ttaccagcac cattaccacc gatgatagag ataaaatcac cttcttttac atctaagttc
     4981 aacccacgaa ggacatggtt ctcattaact gttccagctt cgaaggtttt atgaatattt
     5041 tggattgata aaattgtaga catgacttcc tcctaagcat ttccattcaa ttttggacga
     5101 cgaatattca atttcttctg caattctggc acatagagaa cggttgccag aaggatagct
     5161 gagaagagtt taacaaggtc tgcatccata cctggaattt ccaaaatcgc taagataatc
     5221 aaacggtaaa caactgcacc gagaacaacc gataggagtc tccaacccaa gcgtaagtta
     5281 cgcaagataa cttctgcaat aatgacagat gctagaccga taacaatggt acctgtacca
     5341 gagttcaaat ccgcatagcc attattctga gtaagaagag caccacaaag ggcaattaga
     5401 ccattcgaca acatataacc gtagattttc atattatcta cattgatacc attggcctca
     5461 ctcattggaa tattatcccc tgttgaacga atagctagac caatttgtgt attgagcaag
     5521 agcgtcaata agcctacaac tgctagtaca aagaccagac caatcaccaa aacagcattt
     5581 gtttttgtca aaccaaaatc ctgcaattgg gtcactagag tttcttgctt aagcagagcc
     5641 acattggctt tgcctaagat tttcaagttg atggagtaga gtcctgtcaa ggtaacaata
     5701 ccggtcaaaa gggctggaat ttttagtttt gtatgaagta aaccagaaat caagcctgca
     5761 cccataccag ctaccatagc cataaaggtt gccagcaagg gattcactcc gttcacaatc
     5821 cctgtcgcac aaacagcagc tccaagcgga taagatccct cagctgtcat atcagcaata
     5881 tctaaaatac gaaacgtcaa gtaaacacca atcgccataa ctgaccaaag caagccttgc
     5941 gagatacttg ataaaataat atccacgatt ttctcctttt tctaaattcg tagtcaatac
     6001 taggttctat cttaccgatc ttattcagat acagcaagct tagaagtatc gatacctaat
     6061 ttctgagcca tttcttcatt aacgtgaaga ctgacggtct ctgggtattc tactgccgtt
     6121 tctgacacat tggctccttc gataatctta atagccattt ttgcagtttg acgacctaaa
     6181 gcttcatagt tggtaccgta agtcaaaaga cctccttcat ccaccatgtc cgttgaacca
     6241 ccgattactg gaaccttatg ctccacagat aattcaccaa tcatggtcat ggttgaagcg
     6301 acagtattgt ctgttggaat aaagagagca tctacatcct tcatcaagct agttgtcgca
     6361 tcttggactt catttgaaga tgtgattcct tttactacta ccttataacc tgctttttct
     6421 agcaattcct tagcttgctc tacctggact tctgaattcc gttcgctagt agtgtacaaa
     6481 ataccaacgg tcttagcatc aggaacagct tgtcctagca attcaacctg tttgtcaatt
     6541 ggagcttgat cagatgtacc tgtcaaaagt ccacctggtt tttcaatgga ttctaccaag
     6601 tcagctgaca agggatctgt aaccgcagta aagacaatcg gcgtttcagt agacacagtc
     6661 gctaaactct gggcagacgg cgtagcgatt gccaagacaa tatcatttcc atcgattaat
     6721 tgttctgaaa tggtttgaag gttagcttga tcgccttggg cattttgata atccaaaacc
     6781 aatttttcac cctctttata accatttgct tctaattccg ccacaaaacc ctcacgggca
     6841 gctgtcaatg attcatgctc catatattgg agaactccaa cttttaccac atctgaggaa
     6901 gcttgttctg atgatgagga acaagctgca agtgttagca aacccatact tgctactgaa
     6961 atagttgcca atttttttac tagcttataa tacataaacg tttcttctcc taaatatttt
     7021 actcttctaa tgcttttata gtcgctgcat caataccaac agctttagcc atttcttcat
     7081 tgacggtaat agccgctgta tttggttttt caactgctaa gtcagcaggc ttttcaccct
     7141 tcaaaatctt cacagccaat tcaccagctt gaacaccgat agcatggtag tcgacaccgt
     7201 aggtaaatag agctgcgtca ataactgccg cgtcacttcc catagctgga acttttgctt
     7261 cttttaaaat atcaccgatt gttgaagctg tagaggcaac tgtattatca gttggaagat
     7321 agatagcatc gacttttcct gcaagtgaag taatagcttg ctgaacatcg tttgtcgtcg
     7381 taactgttgt cacttctact tttacacctt ttgcttcgag tgctttttta gcctgctcag
     7441 cttgaacttc tgagtttacc tcgctagaat tgtagaaaat accgacagtc ttcgcagttg
     7501 gtacaacttt gagcaacatc tcgatttgac cttccacatc agttgcatca atcgatcccg
     7561 tcatggtccc acctggtttt tccaatgatt caaccaaacc tgcttcaact ggatctgtga
     7621 cagctgtgaa tacacttgat gtttcactat ctgcattaag catggtttgg gctgccggtg
     7681 ttgcgatggc aaagttaaga tcattttttc ctgccaattt ctcaaccatt gtctgtaagt
     7741 ttgcttgatc accttgagca ttttgcaaat caatcgtcag attttcacct tccttgtagc
     7801 ctgcttcttc gagcgcctct acaaagcctt cacgagctga gctcaacgct tcatgttcag
     7861 catattgaat aataccaaca ttgactgaac ttgaatctga cgaattggaa ctacatgcag
     7921 caagcgttac tgctgacaaa agtgctactg aactaagtat aattt
//
LOCUS       DBNFCK010000086         7873 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.086, whole genome shotgun sequence.
ACCESSION   DBNFCK010000086 DBNFCK010000000
VERSION     DBNFCK010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7873
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.086"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            3..902
                     /gene="mvk"
                     /locus_tag="AC1PIW_001447"
     CDS             3..902
                     /gene="mvk"
                     /locus_tag="AC1PIW_001447"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HIP6606374.1"
                     /translation="MMGEHAVVYGHPAIAMPFSAVEIIAQVTAQGEALTVACDFYKGL
                     VHKMPKIWESLKHAIRFSLYRIGAPTDPAIHIEISSTIPAERGMGSSAAVAVAVARAL
                     FAYYEKELTDSELWDIVQSSEKIAHGNPSGIDAATTSGKSPVFFIKHQPIEPFELKLH
                     AHLVVADTGVTGNTLEAISDVADLLEKKPEAIKLVEELGNLTRQAKEDLATDQAELLG
                     SRMNQAHALLQKLGVSDPSLDKLVSLAQENGALGAKLTGGGRGGCMIALARTAQDAQK
                     LAHILDQAGARQTWIQYLGETND"
     gene            895..1920
                     /gene="mvaD"
                     /locus_tag="AC1PIW_001448"
     CDS             895..1920
                     /gene="mvaD"
                     /locus_tag="AC1PIW_001448"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HIP6606375.1"
                     /translation="MTKQIGIARAHTNIALIKYWGKRDKELFLPMNSSLSLTLDAFYT
                     DTKVVFDPELTADEFYLNGILQKEKEILKISRFLDLFCEYIGERAFARVESLNFVPTA
                     AGLASSASAFAALALATATALDLDLSPATLSTLARRGSGSSTRSLFGGFVEWGMGTGS
                     EDSMAHPIDDADWDIGMVVLAVNTGPKKIASREGMDHTVATSPFYTAWVETAKQDLVD
                     IKAAIASRDFEKLGQITEHNGMKMHATTLSANPPFTYWSADSLVAQEAVRQVREATGL
                     SAYMTMDAGPNVKVLCRASQMDELVAGLAKVFPREKIITSKPGPAAYVLSEKDWQASQ
                     TAFEKGI"
     gene            1922..3001
                     /locus_tag="AC1PIW_001449"
     CDS             1922..3001
                     /locus_tag="AC1PIW_001449"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HIP6606376.1"
                     /translation="MKVQVKIPGKLFLAGEYAVVEAGYPAVIAAVGQYLTVTIETSDQ
                     GSLHSSQKADLYLTWERKEGAVRIQGSHPYALIESAMQVTEAYLTAKGYACHGTYSLS
                     VQSDLDDQASGAKYGLGSSGAVTVATVKALLTYYGHQADALLTYKLAALTQSKLGMTG
                     SFGDLAASSFGGLIAYHSLDRSWLLGKMAELSLLDVVESDWQSLSISPIQLPQGLDLL
                     VGWTGSAASTDSLVSQMESQKSQTEKEQIHSQFLADSKTCVEQLIVACQTNDSVSARQ
                     ALSANRKLLQDFARGMGLVIETPQLSQLCDLAQTYGAVAKSSGAGGGDCGICLVDSKE
                     QKAAIETAWQEAGIFPLTLNIASRE"
     gene            3012..4109
                     /gene="fni"
                     /locus_tag="AC1PIW_001450"
     CDS             3012..4109
                     /gene="fni"
                     /locus_tag="AC1PIW_001450"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HIP6606377.1"
                     /translation="MFYLGEFGLDRKDQHVGLANQQYSATPAKDFTETLFVHHSLPQT
                     KVDEVDISTSVAGLDFAFPFFINAMTGGSKKTREINRLLGIMGHFGKIALASGSVSAA
                     IKDPSVAETFSVMRRENPYGIIFANLGAHHSVENAKRAVDLLEANAIQIHVNAPQEIV
                     MPEGDRDFTMWLKNIETLVREMEVPVIVKEVGFGMSRETVAQLASAGVQTIDVSGTGG
                     TDFAKIENARRTFNDYAYLEGWGQSTVTSLVEAMSVSEEVCPSLIASGGIKTPLDIVK
                     SLALGADLVGMSNHFLQYVKDGKGHRFDDGLQAIKVYQWQIAEIMTMLGAKNIAELRQ
                     KDLVLAPNVQNWCEARGIDWKAYARRSANVS"
     gene            complement(4204..5952)
                     /locus_tag="AC1PIW_001451"
     CDS             complement(4204..5952)
                     /locus_tag="AC1PIW_001451"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6606378.1"
                     /translation="MLSKTKQASVFLRLLSYLKAYRLLTVLALSFLLLTTVVRSLIPL
                     LASYFIDHYISTISETAVAILAAYFALYLLQMVLQYFGNLWFAKVSYSIVRDIRRDAF
                     GRMEKLGMAYFDQTPSGSIVSRITNDTESISEMFSGILSSFISALFIIVTTLSTMFAL
                     DWKLTSLIVLFLPVIFILVDQYRKRSAPIVAKTRSLLSAINSKLAESIEGIWIIQAFS
                     QEERLKEEFEAINQEHLDYASRSMALDSLFLRPALSLVKILAYALLMTYFGLDWSQAG
                     ISAGLIYAFIQYVNRLFDPLLDVTQHFSTLQTSMVSAGRVFTLMDQTIEEPLQLDRGA
                     KIDKGHIVFEDVYFSYDGKRQILDKVSFEVRQGQTIAFVGATGSGKSSIINVFMRFYE
                     FQSGRILIDGQDIRSFSQEELRSAIGLVLQDPFLFHGTIASNIQMYQDISQEEIEEAA
                     RFVDAAPFIEKLPEKYDHLVTERGSAFSTGQRQLLAFARTMASQPKILILDEATANID
                     SETEKTVQTSLEKMRKGRTTIAIAHRLSTIQDADCIYVLDKGKIIESGNHEELLALKG
                     HYHRMYQLQAGQLTAT"
     gene            complement(5942..7684)
                     /locus_tag="AC1PIW_001452"
     CDS             complement(5942..7684)
                     /locus_tag="AC1PIW_001452"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6606379.1"
                     /translation="MKIIKQLWWFFSLEKKAYLIGILSLCLVSLLNLLPASIMGQLID
                     QIASGQLTEHRLLLGVAGLILSALAMYGLRYLWRMNILATSYRLGKIMRARLFDHFMH
                     LSPSFFQQHRTGDLMAHATNDINALTRLAGGGVMSFVDATVTALVTLITMSLSISWQM
                     TLVAILPLPFMTLTTNFLGRRTHENFKASQAAFSELNNKVQEAVSGIKVTKSFGYQEQ
                     ETAAFQEVNQAAFLQNIKTMRYDALFNPAVLFFIGLSYLLTLLVGSHFISQGQVSLGQ
                     LVTFMTYLDLLVWPLMAIGFLVNISQRGDVSYNRIQTLLSISSEVIETDQPLPAPSNG
                     ELVYEISEFAYDNIPVLQDIQFQIKKGQTIGLVGLTGSGKTSLLKLLMREYDVINGQI
                     LLNQENIKNYKLADLRRLIGYVPQDQFLFATSITENIRFGNPDLSLNQVEEAARAVHV
                     YEDIQDMPDRFETMVGEKGISLSGGQKQRLAMARAMILNPDILLLDDSLSAVDAKTEH
                     AILETIKQERLDKTTIITAHRLSAIVHADLILVLEDGKIVERGRHQELLDEKGWYYDT
                     YMMQQLEKEESDAL"
BASE COUNT         2147 a         1754 c         2010 g         1962 t
ORIGIN      
        1 ttttgatggg agagcatgcg gtggtctatg ggcacccagc aattgctatg cctttttctg
       61 ctgttgaaat tattgctcag gtaactgctc aaggggaagc gctgactgtt gcttgtgact
      121 tctataaagg cttagttcac aagatgccaa aaatctggga aagcctcaag catgccatcc
      181 gtttctcgct ctatcgtatc ggagcgccga cagacccagc tattcatatc gagattagtt
      241 ccaccattcc tgccgagcgt ggcatgggct ccagtgcagc cgtggcagtt gcagtagcac
      301 gcgccctctt tgcctattat gaaaaagagc tgaccgacag cgaactctgg gacatcgtcc
      361 agtcttcgga aaaaattgct cacggcaatc cctcgggtat tgatgcggcg accaccagcg
      421 gcaagtcccc cgtcttcttt atcaagcacc agcccatcga accttttgag ctgaaactcc
      481 atgcccattt ggtagtggct gacacaggtg tgacgggaaa taccttagaa gccatttcag
      541 acgtggcaga tttgttggag aaaaagccag aggctatcaa gctagtagaa gaactgggga
      601 acttgacccg acaggccaag gaagatttag ccacagacca ggcagagctt ctaggcagca
      661 gaatgaacca agctcatgcc ctcctgcaaa aattaggcgt gtctgatcct agcctagata
      721 agctagtcag ccttgcccaa gaaaatggag ccctcggagc caaattaact ggtggcggac
      781 gaggcggttg catgattgcc ctggcaagaa cagctcagga tgcccaaaaa cttgcacaca
      841 tccttgacca agcaggcgcc cgccaaacct ggatacagta cttaggagaa accaatgact
      901 aaacaaatag gcatagcccg tgcccatacc aatattgcct tgattaaata ctggggaaaa
      961 cgcgataagg aattattcct acctatgaat tccagtttat ccctgaccct tgacgcattt
     1021 tatacggata ccaaggtcgt ttttgaccca gaattaactg ctgatgagtt ctatctcaat
     1081 gggattttac aaaaagaaaa agaaatttta aaaatttctc gatttttgga tttattttgc
     1141 gaatatatag gtgaaagggc atttgcccga gtggaaagtc taaattttgt tccgactgca
     1201 gctggtttgg caagctcagc atcagccttc gcagcgcttg cgcttgcaac cgcaactgcc
     1261 ttggatttag atttgtcacc agccacccta tcaacccttg ctcgcagggg ttctggctct
     1321 agtacccgta gcctattcgg tggatttgtt gagtggggca tgggaactgg ttcggaagat
     1381 tcgatggctc atcccattga tgatgcggat tgggatatcg gcatggtcgt cttggcggtc
     1441 aataccggac cgaaaaagat tgctagtcga gagggaatgg accacacagt tgccacctca
     1501 ccattttaca cagcctgggt agaaaccgct aagcaagact tggtggacat caaggcagct
     1561 attgccagcc gtgattttga aaaacttggt cagattacag agcacaatgg catgaaaatg
     1621 catgcaacaa cgctctctgc caatccaccc tttacctatt ggtcagccga tagcctagtg
     1681 gctcaagagg cagtccgtca ggttcgtgaa gcaactggct tatcagccta catgaccatg
     1741 gatgcaggac caaatgtcaa ggtcctctgt cgagcaagtc agatggatga gctagtagct
     1801 ggattggcca aagtcttccc aagagagaaa atcatcacca gcaagcctgg acctgctgct
     1861 tatgttctca gtgagaagga ttggcaggca tcacaaacag catttgaaaa gggaatctag
     1921 catgaaagtg caagtaaaga taccaggaaa actctttctg gcaggggaat atgcagttgt
     1981 tgaagctggc tatcctgcgg tgattgcggc ggttggccag tacctaaccg tcaccattga
     2041 aacaagtgac caaggcagtc ttcattctag tcaaaaggca gacctttatc tgacctggga
     2101 gcgtaaggaa ggtgccgttc gtattcaagg tagccatccc tatgccttga ttgaatcagc
     2161 tatgcaggtg acagaagcct atctgacggc caagggctat gcttgtcatg gaacctatag
     2221 cctatctgta cagtccgacc tagatgacca agcctctggt gccaagtacg gtctggggtc
     2281 ttcaggagca gtaacggttg cgacggtcaa ggccctgttg acctattacg gtcatcaagc
     2341 ggatgccctt ttgacctaca agctagcagc tctgacccag tccaagctag gcatgacagg
     2401 ttcctttgga gatttggcgg cttccagttt tggcggcttg atagcctatc attccctgga
     2461 ccgttcttgg cttttaggga aaatggcaga gctgtctctg ctggatgtgg tggaaagtga
     2521 ttggcaaagt ttgtctatca gccctatcca gttaccccaa ggtctagacc tcttggttgg
     2581 ttggacaggg tcggcggctt caacggatag tctagtttcc cagatggaaa gccaaaaaag
     2641 tcagacagaa aaagagcaaa ttcatagcca gtttttagcc gactccaaga cttgtgtaga
     2701 gcagttgata gtggcctgtc agacaaatga ttcagtatca gctagacagg ccttgtcagc
     2761 taatcgcaag ctcttacagg actttgcgag aggaatgggc ttggtcattg aaacgcctca
     2821 actgagtcaa ctctgtgatc tggcccaaac ttatggagcg gtggccaaat catctggtgc
     2881 tggcggaggt gactgcggta tttgcttggt agacagtaag gaacaaaaag cagcgataga
     2941 gacggcttgg caagaagccg gtatttttcc actcacccta aacatcgcaa gtagagaata
     3001 aggagaaccc tatgttttat ctaggagaat ttggtttaga tcgcaaggat caacacgttg
     3061 gcttggccaa tcagcaatat agtgccacgc cggccaagga ctttacagaa actctgtttg
     3121 tccatcattc cctgccgcag accaaggtgg atgaagtgga tatttcgacc agtgtagctg
     3181 gtttggactt tgcctttcct ttcttcatca atgccatgac tggtggaagc aagaagaccc
     3241 gggaaatcaa ccgtctgttg ggaattatgg ggcattttgg caagattgcc ctggcttctg
     3301 gttcggtcag tgcggcaatc aaggatccgt ctgttgcgga aaccttctct gttatgcgtc
     3361 gggaaaatcc ttacgggata atctttgcca acctaggtgc ccatcatagt gtggaaaatg
     3421 ccaagcgggc ggtggatttg ttggaggcca atgccattca gatccatgtc aatgctcctc
     3481 aggagattgt catgccggag ggggaccgtg atttcactat gtggttgaag aatattgaaa
     3541 ctctggtgcg ggagatggaa gtgcctgtca ttgtcaagga agttggtttt ggcatgagcc
     3601 gtgaaacagt agcccagctg gcttctgccg gcgtgcaaac aattgatgta tctggtactg
     3661 ggggaacgga tttcgccaag attgaaaatg cccgtcggac cttcaatgac tatgcctatt
     3721 tagaaggttg gggccagtct accgtgactt ccttggtgga agctatgtct gtttctgaag
     3781 aagtttgccc aagcctgatt gcttctggtg gcattaaaac accgctagat attgtcaaat
     3841 cattggcttt aggtgcagac cttgtcggta tgtccaatca tttccttcaa tatgtcaagg
     3901 atggaaaagg gcatcgtttt gacgacggtt tgcaggctat taaggtctat caatggcaga
     3961 ttgctgaaat catgaccatg ctgggtgcta agaatattgc ggaactccgg cagaaagatc
     4021 tggtcttggc accaaatgtc cagaactggt gtgaggcgcg tgggattgat tggaaagcct
     4081 atgcccgtcg gtcagcgaat gtgagttaaa gagagataga ttgatgtctt cagttctcaa
     4141 caatgtagaa aaataaaaaa gccaggtcca ttttggaact tggcttttgt catggagtgg
     4201 tttctaggtt gctgttaact ggcctgcttg taactggtac atacggtggt agtgaccctt
     4261 gagggctaga agttcttcat gattgcctga ctcgatgatc tttcccttat ccaagacata
     4321 aatacagtcg gcatcctgaa tggtcgatag gcggtgggcg atggcaatgg ttgtccgccc
     4381 ttttcgcatt ttctcaagag aagtctggac tgtcttctcc gtctcagaat cgatattggc
     4441 ggtagcctcg tctaaaatga gaatcttagg ctggctagcc atggtgcgtg caaaggccag
     4501 cagttgccgt tgtcctgtcg aaaaggcgga gccccgctcg gtgaccaagt ggtcgtattt
     4561 ctcaggaagt ttttcgatga agggagcagc gtccacaaat cgagccgctt cctcaatctc
     4621 ctcctgagaa atatcctgat acatctggat attggatgca atggttccgt gaaatagaaa
     4681 gggatcttgc aggactaggc caatcgcgga ccgcaattct tcctgcgaaa agctacgaat
     4741 atcctgaccg tcaatcaaaa tccgtccaga ttggaattca tagaaacgca taaagacatt
     4801 gatgatagaa gatttgcctg atcctgtagc acccacaaag gcgatggtct gcccctgtct
     4861 cacttcaaag gaaaccttgt ccagaatttg ccgcttgccg tcataggaaa agtaaacatc
     4921 ctcaaagaca atatgcccct tgtcaatctt ggcccctcgg tccagctgta gtggctcctc
     4981 gatggtctgg tccatgagcg taaagacccg tccggcagat accatagagg tttggagggt
     5041 ggaaaaatgc tgcgtgacat ccagcaaggg atcgaaaaga cgattgacat actggatgaa
     5101 agcatagata agaccagcag aaattccagc ctgtgaccag tccaaaccaa aataggtcat
     5161 gagtagggca taggccaaaa tcttaacaag tgacagggca gggcggagaa agagactgtc
     5221 caaggccatg gaacgactgg catagtccag atgttcctgg ttgatagcct caaactcttc
     5281 ctttagcctc tcctcttgag aaaaggcctg gataatccaa atcccctcga tgctctctgc
     5341 cagtttgctg ttgatggcac tcaacagact tctagtcttg gccacaatgg gagctgaccg
     5401 cttccgatac tgatcaacca ggatgaaaat cacgggcagg aagaggacaa tcagactggt
     5461 caatttccaa tccagagcaa acatggtcga aagagtcgtg acgataataa agagagctga
     5521 gataaaactg gataaaatcc ctgaaaacat ctctgaaata ctctccgtat cattggtgat
     5581 gcgagagaca atggaaccac taggagtctg gtcgaagtaa gccattccca gcttttccat
     5641 tctcccaaag gcatcccggc gaatatctct gacaatgctg taggaaactt tagcaaacca
     5701 gagatttcca aaatattgaa gtaccatttg aagcagatag agggcgaagt aggcagctag
     5761 aatggccaca gcagtctcag aaatagtaga aatgtagtgg tcgataaaat aagaggctag
     5821 aaggggaatt agactgcgga ccacggtagt cagtagcaaa aagctcaagg caaggactgt
     5881 cagcagtcga taggctttga gataggacaa gagtcgcaaa aaaacactag cttgcttggt
     5941 tttagagagc atcactttcc tccttttcga gttgctgcat catataggta tcgtagtacc
     6001 agcctttttc atctagtaat tcttggtgac gacctcgttc tacgattttt ccatcttcaa
     6061 ggaccagaat caaatcggca tggacaatag ctgacaggcg atgggcggtg ataatggtgg
     6121 ttttgtcaag gcgttcttgc ttgatggttt cgagaatagc gtgttctgtt ttagcatcca
     6181 ccgctgagag cgagtcatcc aagagcagga tatctggatt gagaatcatg gcacgggcca
     6241 tagccaaccg ttgtttttgt ccgccagaaa gggaaatccc cttttcgccg accatggttt
     6301 caaagcggtc gggcatatcc tgaatatcct catagacatg gacagcccga gcggcctctt
     6361 ctacctgatt tagagataaa tcgggattgc cgaagcggat gttttcagta atgctggtcg
     6421 caaagaggaa ctggtcctgg ggtacatagc caattaagcg tcgcaggtca gctagcttgt
     6481 agttcttgat attttcttga ttgagcaaga tctgtccatt tatcacatcg tattctcgca
     6541 tcaggagctt gagcaggctt gttttcccag aacctgttag ccccacaaga ccaattgtct
     6601 gccctttttt gatctgaaac tggatgtctt ggaggactgg tatgttatca taggcaaatt
     6661 cgctaatctc atagacaagc tctccattgc ttggtgcagg cagcggttgg tctgtttcaa
     6721 tgacctctga ggaaatgctg agaagggtct gaatccgatt gtaagataca tcaccacgct
     6781 ggctaatatt gaccaaaaaa ccaatcgcca taagaggcca gacgaggaga tctaaatagg
     6841 tcatgaaagt tactagttgg cccagactga cctgaccttg cgagatgaag tgagagccga
     6901 ccagaagggt cagcaggtag gacaggccaa tgaagaagag gacggcaggg ttgaacaggg
     6961 catcgtaacg catggttttg atattctgca agaaggctgc ttggttgact tcctgaaaag
     7021 ccgccgtttc ttgttcttgg tagccaaagg atttagtaac ctttatacca gaaacagctt
     7081 cctggacctt gttgttaagc tctgaaaagg ctgcctgtga agccttgaaa ttttcatgag
     7141 tgcgacggcc taaaaaatta gtggttagag tcatgaaggg tagggggagg attgcaacca
     7201 aggtcatctg ccaggaaatg ctcaaactca tcgtaattag ggtaaccaag gctgtgaccg
     7261 ttgcatcaac aaaagacatg acgccaccac ctgctagtcg ggtcagggca ttgatatcat
     7321 tggttgcgtg ggccattaaa tccccagtcc gatgttgctg aaaaaaggat ggagataggt
     7381 gcataaagtg gtcaaacagc cgagccctca tgatttttcc tagacgataa gatgttgcca
     7441 ggatgttcat tcgccaaaga tagcggaggc cgtacatggc caaggctgac aagatgagcc
     7501 cagctacccc gagtagcaag cgatgctcag tcaactgtcc gctggcaatc tggtcaatca
     7561 actgccccat gatagaagcg ggaaggagat tgaggaggct gaccaaacag agtgataaaa
     7621 tcccaatcag ataggccttt ttctcaaggg aaaagaacca ccacaattgc ttgataatct
     7681 tcataaatgt cctttctgaa caaaagaaag gttggtagct tgtcgcaacc aacaaataat
     7741 agaattcggt gacactagtg tatcacagac tggtagaaat agaaagtttg taacttaaaa
     7801 attaggcaac tctagccgta gccgaataga agaaacagtt ggagttgtaa acacgtacga
     7861 tacaggtaga aat
//
LOCUS       DBNFCK010000087         7812 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.087, whole genome shotgun sequence.
ACCESSION   DBNFCK010000087 DBNFCK010000000
VERSION     DBNFCK010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7812
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.087"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            53..1327
                     /locus_tag="AC1PIW_001453"
     CDS             53..1327
                     /locus_tag="AC1PIW_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIP6606380.1"
                     /translation="MKKIGRYVTLLAAAGLLAACSTSTESSKTTGGSSSGKTEISYAI
                     WDSGQEPGLRKIADEFEKKNPDIKINIQVSDWDSYWTMLEAGATGGSLPDTFWMHSNE
                     IYRYGSNEMLLPLDEYLAKSEDAKLANFPDGLNEIYNINGKQYAIPKDFDTIGLWYNK
                     KLFDEAGIPYPDDTWDWNKLKEVAKKLTKPDGSQYGFGAGLSNQEGYYNFIYQNGGKV
                     ITDDLKSGYADPKTIEALDYYFSFVKEKISPAITVDKERAEAFQNGQVAMSVFGSWNL
                     SGFTANDYIRENADVAVLPKGPDGTRATIFNGLGHAIAATTKHPDAAWKWVEYLSSKE
                     AQEMQATLGVAISAYKGAADTWVDSNKNFTIKNYVDMVDYAQIRPYSQTTIKWEDKAY
                     ELLKPAYLGEKATEEAAKETADMMNAELATEK"
     gene            1396..2289
                     /locus_tag="AC1PIW_001454"
     CDS             1396..2289
                     /locus_tag="AC1PIW_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIP6606381.1"
                     /translation="MFRKKGSLNEAIWGWGMVAPTIIGLIVLNIIPIFQTMKMSFHKS
                     GDFGKGDIFVGLANYQRMLGDAQVWQATWNTLKYTILVVPATVALAMLLAVLLNSKIK
                     GKHIYRTIFFLPMVAAPAAVTMVWKWLYNTDFGLINYILRRLGLGAVNWIEDPKIALY
                     SIALIGIWSTVGYSMILILAGLQEIPTDFYEAARIDGASPAKQFFSITLPLVSPTLFF
                     VVVTSVIQSMQVFDVIYMMEDVRSPAYDKTVSLVYLFYNNSFKYSDKGYGSTIVMLLL
                     LIILVITFVQMKVQKKWVHYR"
     gene            2300..3130
                     /locus_tag="AC1PIW_001455"
     CDS             2300..3130
                     /locus_tag="AC1PIW_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIP6606382.1"
                     /translation="MKQSAFKSTIWMHAFLILVAMGMLIPFVWMVLTSFKTVTESTQM
                     NPFHFLPSNWMVSNYTEAIRTNNFPILYLNTILMMLWRIFSSVMFSAMAAYAFARLEF
                     PGRNFLFGLVLFQMMVPPQLFVIPQYLMIDQLGMRNTIFALVFPGIVSAFGTFLLRQF
                     FMGLPKELEESAKLDGCNIGQTFFKVMLPLAKSGLIALAIFTALFAFKDLLWPLIINS
                     EADKATLSSALSKIQGAYAVNYPQLMAASVLAIWPMLVLYVIFQKQFIQGIATSGGKL
                     "
     gene            3140..5341
                     /locus_tag="AC1PIW_001456"
     CDS             3140..5341
                     /locus_tag="AC1PIW_001456"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004557 - alpha-galactosidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HIP6606383.1"
                     /translation="MGIIFHEKTKEFHLYNEFISYVLTVLPNGHIGSLYYGKRVTETA
                     SYQYVREDEYRALTSFVEDDDRFFSLQYANPEFACYGTTDYFSPTFELVQKDGSSLSH
                     FVYQGHAIYAGRSALKGLPHLYLDEEGQADSLDIYLLDEDSQTRLVLSYTIFRDYPAV
                     TRSARFEQLGEKSVCLNRALSMTLCLPDMDYDWLHLDGAWGRERHLQLSPLHQGCQSI
                     YSLKGASSAEHNPFMALKRPTADEQQGEVLGFSLVYSGNFLAQVDVSSFQKTRVSMGI
                     HPERFSWTLDKGEEFQTPEVVMVYSEHGLNGMSQTYHRLFQKHLVRGYWRDRERPVLL
                     NNWEAMSFDFDEETILSLAKEAAGLGVELFVMDDGWFGKRNHDRAGLGDWTVNREKLP
                     SGLTGIIDQVHAMGMKFGLWIEPEMVNRDSELYRAHPDWIFHHPQHSQSHGRHQYTLD
                     LSREDVYQNIHDQLHRLLAEHDIDYIKWDMNRYMTEVFTCTREADRQGETFHRYILNL
                     YRLYDSLIAAFPTVLFESCSSGGARFDPGMLYYAPQTWVSDDTDAMERLKIQYGTSMV
                     YPLNSMGCHVSASPNQQLGRATPLNTRANVAFFGSFGYELDLFECSQEELEEMKEQIA
                     FYKTHRKIFQQGIFTRLKSPFDGEITAWQVQSSDGQQVIVGYYRRLTTANLSYQRLYL
                     QGLEEEAVYHLDGEVYTGSQLMHTGLSIRHGDHVGDNKDFTSCLMVLQKQQ"
     gene            5616..6317
                     /locus_tag="AC1PIW_001457"
     CDS             5616..6317
                     /locus_tag="AC1PIW_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HIP6606384.1"
                     /translation="MKKYAFREGMRDAIPTVLGYASIGLACGVVSVNSGISTVEMGLM
                     SLLIYAGSAQFVMCAMIFAGAPLLSIAVTVFFVNLRNFLMCLHATTIFQGNRLGSNIL
                     IGSFVTDESYAVLLRKQIEHHTITPEWMYGNNFASYASWVTFTTLGNLIGGLIPNPEQ
                     FGLDFALVAMFVGIFAGQLEAMVRTIPLKKIGLILLAVGLAYIGLSMMVSSYVAVLLA
                     TLLGCFVGVMIDDKN"
     gene            6304..6627
                     /locus_tag="AC1PIW_001458"
     CDS             6304..6627
                     /locus_tag="AC1PIW_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HIP6606385.1"
                     /translation="MIKTNILLIILMAALVTWVPRVLPFVLTQNKSLPPKLVKFLSFL
                     PITIIFALTLSSIMDEKVGSLPSFLPVESLAILPTFFVVLKTKNILLAVLVGVVTTAL
                     LRLIF"
     gene            6637..7659
                     /locus_tag="AC1PIW_001459"
     CDS             6637..7659
                     /locus_tag="AC1PIW_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HIP6606386.1"
                     /translation="MVKENAKGVGLCFLLALVGQWLGHLFPLVGGPVFALLIGMGLYP
                     YFSSKNAFQSGLTFTSKKILQYAVICLGFGLNLSAVLAVGRQSLPIILSTISFALILA
                     FLMWKILPISSHLATLIGVGTSICGGSAIAATAPIIQADDEDVAQAISVIFLFNVLAA
                     LVFPSLATWLGFSTDSGQAFGMFAGTAVNDTSSVTAAASTWDSLYGLGSQTLDTAVTV
                     KLTRTLAIIPITTVLAIWQTRGKGLKADKKSLLAGFPTFILYFILASLITTIAGGFGI
                     GADWFAPLKSLSKFFICMAMVAIGLRTNVFALVKNGRAALMVGLVCWLGVTVLTLVWQ
                     AILGIW"
BASE COUNT         1987 a         1461 c         1894 g         2470 t
ORIGIN      
        1 tagaaataac aaagttcgtc gacgtattcg acaaaagaaa aggagtatta tcatgaagaa
       61 aattggtcgc tatgttacct tgcttgcggc tgcaggcctg ctggcagcgt gttctacttc
      121 gactgagtct agcaaaacta cgggaggaag tagttcagga aagacagaaa tatcctatgc
      181 tatttgggat tctggtcaag agcctggttt gagaaagatt gcggatgagt ttgagaaaaa
      241 gaatccagat attaaaatca atattcaagt atcggactgg gattcctact ggaccatgtt
      301 agaagctggt gcgacaggtg gttcattgcc tgataccttc tggatgcatt caaatgagat
      361 ttatcgctat ggttccaatg aaatgctatt gccattggat gaatacttag ccaagagtga
      421 agatgccaaa ctagctaatt tcccagatgg tttgaatgaa atctacaaca taaatggtaa
      481 gcagtacgct attccaaaag atttcgatac aatcggtctt tggtacaata aaaaattatt
      541 tgacgaagca ggcattccat atccagatga tacttgggat tggaataagt tgaaagaagt
      601 agcgaagaaa ttgaccaagc cagatggtag tcagtatggt ttcggtgctg gtttgagtaa
      661 ccaagagggc tactacaact ttatctatca aaatggtggt aaggttatta ctgatgattt
      721 gaaatcaggc tatgctgatc ctaagaccat tgaggctttg gattactact ttagctttgt
      781 caaagaaaaa atttcaccgg ctattacagt agataaagag cgggccgaag cattccaaaa
      841 cggtcaagtg gctatgagcg tcttcggttc ttggaattta tctggtttca ctgctaatga
      901 ctacattcgt gaaaatgctg atgtagctgt tttgccaaaa ggtccagatg gaactcgtgc
      961 aaccatcttt aatggattgg gtcatgccat tgctgctacc actaaacatc cagatgctgc
     1021 ttggaaatgg gttgaatacc tcagctcaaa agaggcacaa gaaatgcagg caacacttgg
     1081 tgtggcaatc tcggcttata aaggcgcggc agatacttgg gtagattcaa acaaaaactt
     1141 tactatcaag aattatgttg acatggtgga ttatgcacag attcgtccat attcacaaac
     1201 aaccatcaag tgggaagata aggcttatga gctattgaaa cctgcttatc ttggagaaaa
     1261 agcaactgaa gaagctgcca aagaaacagc tgatatgatg aatgctgaat tagcaactga
     1321 aaaataggta ggaggtcttg ggaagttgat tcttccccgg accttcttat taaaatgaag
     1381 aaggatgggg ataagatgtt ccgaaaaaaa ggaagtttaa atgaggcaat ttggggatgg
     1441 ggaatggtag caccgaccat tattggatta atcgtgctca atattattcc tattttccaa
     1501 accatgaaga tgagttttca taagagtgga gatttcggca aaggtgacat ctttgtcggc
     1561 ttggcaaatt accaacgcat gttaggggat gcgcaagtgt ggcaggcgac atggaatacc
     1621 ttgaaatata cgattctagt tgttccggct accgttgcct tggccatgtt gcttgcagtt
     1681 ttgcttaatt ctaagattaa aggtaagcat atttatcgaa ccattttttt cttgcctatg
     1741 gtggcagcac cagccgcggt gaccatggtt tggaaatggc tctacaatac ggacttcggc
     1801 ttgattaact atatcttgcg tcgtttggga ctgggtgctg tgaactggat tgaagatcca
     1861 aagattgctc tatactcaat cgctttgatt ggaatttgga gtacggttgg ctatagtatg
     1921 attttaattt tagctggttt gcaagaaatt ccaacggatt tttatgaggc agctcgcatt
     1981 gatggagcta gtccggccaa gcaatttttc tccattaccc tgccattggt ttcaccaacc
     2041 ctgttctttg ttgtggtaac cagtgtcatt caatccatgc aagtctttga tgttatctat
     2101 atgatggagg atgttcgtag tcctgcctat gataagacag tatctctggt ttatctgttt
     2161 tacaataatt cctttaaata ttcggataag ggctatggct ccaccattgt catgttgttg
     2221 ttattgatta ttcttgtcat tactttcgtt cagatgaagg tacagaaaaa atgggtgcat
     2281 taccgttaag gaggttcatg tgaaacaatc agcttttaaa tcaacaatct ggatgcatgc
     2341 cttcttgatt ttagtggcaa tgggcatgct gatcccattt gtttggatgg tgttgacgtc
     2401 ttttaaaacg gtaaccgaat ccactcagat gaatcctttc cactttttac caagtaactg
     2461 gatggtgagc aattatacgg aggctattcg gactaataat tttccgatac tctatctgaa
     2521 caccattttg atgatgttat ggcgtatctt tagctcggtt atgttttcag ctatggcagc
     2581 ttatgccttt gctcgactgg agtttcctgg gcggaatttc ttgtttggtc tggtcctctt
     2641 tcaaatgatg gtgccacctc agttattcgt cattcctcag tatttgatga ttgaccaact
     2701 tggtatgcgc aatacgattt ttgctctggt attcccagga attgtcagtg cctttgggac
     2761 attcttgctc cgtcaatttt tcatggggct tccaaaagaa ttggaggagt ctgcgaaatt
     2821 ggatggttgt aatatcgggc agaccttctt taaggtcatg ttgccattgg ccaagtctgg
     2881 cttgattgcc ttagcgattt ttacggcttt gtttgctttc aaggatttgc tgtggccatt
     2941 gattatcaac tcagaggctg acaaggctac cctatctagc gcactttcta agattcaggg
     3001 agcttacgct gttaactatc ctcagctcat ggctgcaagt gttttggcta tttggccgat
     3061 gttggtactg tatgtgatat tccagaagca gttcatccaa gggattgcga catctggtgg
     3121 aaaattatag gaggctacaa tgggaattat ctttcatgaa aagacaaagg aattccatct
     3181 ttacaatgag tttattagct atgtgttgac ggtcttgcct aatggtcata ttggtagttt
     3241 gtattatgga aagcgtgtaa cagaaacagc ttcttatcag tatgtacgtg aagatgagta
     3301 tcgtgctttg acttcctttg tagaagacga tgaccgtttc ttttcgctcc aatatgccaa
     3361 tcctgaattt gcttgttatg gtacaacgga ctatttttct ccgacttttg agttggtgca
     3421 gaaggatggt agcagtctat cgcactttgt gtatcaaggt catgcgattt atgctgggcg
     3481 atcggctttg aagggtcttc ctcatctcta tctggatgag gagggacagg cagatagttt
     3541 ggatatttat ttgttggatg aagattcgca gacccggctg gtcttgtctt atacgatttt
     3601 tcgagattat ccagcggtga ctcgctctgc tcgttttgaa caactcggag aaaaatctgt
     3661 ctgtttgaac cgtgccttga gtatgacact ttgtctgcct gatatggact atgattggtt
     3721 gcatttggat ggggcttggg ggcgtgagcg gcatctgcaa ctatcgcctc ttcatcaagg
     3781 ttgtcagtct atttacagtc tcaaaggggc tagtagtgcg gagcacaatc cctttatggc
     3841 tttgaaacgc ccaactgcgg atgagcaaca gggagaggta ctaggcttct cgctcgtcta
     3901 tagcggaaac tttttggcgc aggtggatgt gagttctttc caaaaaacaa gagtgagtat
     3961 gggaatccat cctgagcgtt tttcttggac attagataag ggagaagagt tccagacgcc
     4021 tgaggttgtt atggtttata gcgagcatgg tttgaatggc atgagccaaa cctatcaccg
     4081 cctcttccaa aaacatctgg tacgtggcta ttggcgtgat agagaacgac ctgttctctt
     4141 gaataactgg gaagccatga gttttgattt tgatgaggaa acaattcttt ccttggccaa
     4201 agaggctgca ggtcttggtg ttgaactctt tgtcatggat gatggttggt ttggcaagcg
     4261 aaaccatgac cgagcaggct tgggagattg gacggtcaac cgagagaaat taccaagtgg
     4321 cttgacaggg attattgacc aagttcatgc tatgggaatg aaatttggac tttggatcga
     4381 acctgaaatg gtcaataggg acagcgagct ttaccgagcg catccagact ggattttcca
     4441 tcatcctcag catagtcaat cgcacggacg ccaccaatat acgctggatt tgtcgaggga
     4501 ggatgtttat caaaatattc atgaccaact ccatcggctg ttggctgagc atgatattga
     4561 ttatatcaag tgggacatga atcgctatat gacagaggtc tttacatgta cacgggaggc
     4621 ggatcgacaa ggtgagacct tccatcgcta tattctcaat ctctaccgtt tatatgatag
     4681 cctgatagca gcttttccga cagtcttatt cgaatcttgt tcaagtggtg gagctagatt
     4741 tgatccaggg atgctctact atgcaccgca aacctgggtc agcgatgaca cggacgccat
     4801 ggaacgcttg aaaatccaat atggaacgag catggtctat cctttgaata gtatgggttg
     4861 ccatgtgtca gctagtccaa atcagcagtt ggggcgtgct actccactaa acacacgtgc
     4921 caatgttgca ttctttggaa gttttggcta tgaattggat ttgtttgagt gtagccaaga
     4981 agagttggaa gagatgaaag agcagattgc cttttacaag actcaccgta agattttcca
     5041 acagggtatt ttcactcgtc tgaaaagtcc attcgatgga gaaataacgg cttggcaggt
     5101 ccaatcctcg gatggtcagc aagtcatcgt gggttattat cgtcggttga caacggctaa
     5161 tctctcctat caacgcttgt atttgcaggg attggaagag gaagcggtct atcaccttga
     5221 tggagaagtt tatactggta gccaattaat gcatacaggt ttgtcgattc gacatgggga
     5281 ccatgttgga gacaataagg actttacttc ttgtttgatg gtccttcaga aacaacagta
     5341 gcatccctat tcctaggccc agctatcggc tgggtctatt ttatgcagaa agtacatcaa
     5401 taagggcaag acctctactt atactcttcg aaaatcaaaa ttatccgttg tcaacttgcc
     5461 ttgatgaact ccagttctat cttcggcttc gtttcctagg ctacttttga ttttcattga
     5521 gtattagata taagaattct tgaaaaacgg gttgcaatgt gagaaaatga gagaataatt
     5581 cttcaatcaa ttagtgaggg gaagggagat aatgaatgaa gaaatatgct tttcgtgaag
     5641 ggatgcgcga tgccattccc actgtattgg gctatgccag tattggtcta gcctgcggag
     5701 tcgtatcggt taattcgggc atttctactg tggagatggg gctgatgagc ctattaatct
     5761 atgccgggag tgctcaattt gtcatgtgtg ccatgatttt tgcaggtgcg cctttgttat
     5821 ccattgcagt aaccgttttc tttgtcaacc tgcgtaattt tctgatgtgc ttgcatgcga
     5881 caactatttt tcaaggaaat cgtttagggt cgaatatctt gataggatcc tttgtgacgg
     5941 atgaatccta tgcagttctt ttgcgtaagc agattgagca tcataccatt actccagaat
     6001 ggatgtacgg aaataatttt gcaagctatg cctcttgggt tacattcacg actctcggca
     6061 atctgattgg tggtctcatt ccaaatcctg agcagtttgg attggacttt gccttggttg
     6121 ctatgtttgt cggtattttt gcaggtcagt tagaggctat ggttcggacc attccgctga
     6181 aaaaaattgg cttaattctg ctggcagtcg gtctggccta tatcggtctg tctatgatgg
     6241 tgtcgtccta tgtggcagtg ctcttggcga cactactagg ttgctttgtg ggggtgatga
     6301 ttgatgataa aaactaacat tttactgatt attctaatgg cggccctggt gacctgggtg
     6361 ccacgcgtcc tgccttttgt cctgacgcaa aataaatctc tccctccaaa gttggtgaaa
     6421 ttcctcagct ttttgccaat cactatcatt tttgcattga ccctctccag tattatggat
     6481 gagaaagttg gctccttgcc aagttttctc cctgtggaaa gcttagctat tcttccgaca
     6541 ttttttgtgg ttttgaaaac aaaaaatatc cttttagcag tcttggttgg cgttgttaca
     6601 accgcgttat tacgtttgat attttaggag gtttttatgg taaaagaaaa tgccaaaggt
     6661 gtcggacttt gtttcctttt ggctttggtg ggtcagtggt taggtcattt atttccactt
     6721 gtaggtgggc ctgtttttgc ccttctaatt gggatgggtc tgtatcccta tttttcaagt
     6781 aaaaatgcat ttcagtcagg cttaaccttt acttccaaga aaattttaca gtatgctgtg
     6841 atttgtttgg gatttggatt aaacttatca gcggtcttgg cagttgggcg tcaatctctt
     6901 ccaattattc tttcgaccat tagttttgct ttgattttgg catttctgat gtggaaaatc
     6961 ttgcccattt catctcatct ggcaaccttg attggcgtag gtacctctat ctgtggaggc
     7021 tctgccattg cggcgactgc tcccattatt caggcagatg atgaggatgt ggcacaagca
     7081 atctcggtca tctttctctt caatgtcttg gcggctttgg tctttcctag tctggcaact
     7141 tggcttgggt tttcgactga ttcgggtcag gcctttggga tgttcgccgg aacggcggtc
     7201 aatgacacct cctctgtgac agcagcggct tcgacttggg atagcttata tggtctaggc
     7261 agtcagacct tagatacagc agtgacggtt aagctaacac gaactttagc cattatcccg
     7321 attacaacgg ttttagcaat ttggcaaacc cgaggaaagg gtctcaaggc agacaagaag
     7381 tccctcttgg caggttttcc gacctttatc ttgtatttta tcttagctag tctaatcaca
     7441 acgatagcgg gtggatttgg tataggggca gattggtttg ctccgttgaa gtccctctct
     7501 aaatttttca tttgtatggc tatggtggcg attggtttgc ggaccaatgt gtttgccttg
     7561 gtgaaaaatg gcagagcggc tctcatggtt ggtctggttt gctggctggg agtgacggtg
     7621 ttgacactgg tctggcaagc gattttgggg atttggtaag agaaatgtga gtgggaaaga
     7681 catcatttcc tatttcaaac aatttttgag aagtaaatcg cagtgccgca gggctttttg
     7741 tcgttcggtt tttagtaccg ttgaacaact taaaactagt tagttttgtt taaaatgtaa
     7801 ttgaattgtc tt
//
LOCUS       DBNFCK010000088         7772 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.088, whole genome shotgun sequence.
ACCESSION   DBNFCK010000088 DBNFCK010000000
VERSION     DBNFCK010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7772
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.088"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            80..832
                     /locus_tag="AC1PIW_001460"
     CDS             80..832
                     /locus_tag="AC1PIW_001460"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6606387.1"
                     /translation="MLLEINHLEKVYRTRFSKEETRALQDVDFKVEEGEFIAIMGESG
                     SGKTTLLNILATLDKPTRGSVILNNQDITKIKESKLADFRLRNLGFVFQDFNLLDTLS
                     IQDNIFLPLVLARESYQEMEKRLEVLAPKLRIQDLLKKRPFELSGGQKQRVAIARSLI
                     TQPQIVLADEPTAALDYRNSEDLLNLFEEINRDGQTILMVTHSANAASHAKRVLFIKD
                     GRIFHQLYKADKSNQDFAKEISLNMSALLGGE"
     gene            833..2803
                     /locus_tag="AC1PIW_001461"
     CDS             833..2803
                     /locus_tag="AC1PIW_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6606388.1"
                     /translation="MFYLKLAGQNIRKSMGVFAPFVLASLVLFTLICSMFLIMLSPVM
                     KTMGSGAISIGLGVIILTIFSVIMEIYSFNFLMKQRAREFGLYNMLGMNKKKVALIAS
                     LELMMIFLLVVVLGSVLAGVFANVLYLIFVNITHYSDLHFGIEPLAFVMTAFLFAGFF
                     FLLELLAIRTIGKTSPLILFRASEKGEREPKGNVLLAALGVFSLGMGYYLSLSSKAEG
                     LAVIYRFFIAVLFVIAGTYLFYISFMTWYLKARRKNKRYFYTPEHFITTSQMIFRMKQ
                     HATGLANITLLAVMAFVTIATTTSLYTGMSSMTGALYPKETSITYKVSDRSQGEMAFQ
                     QSVLSHFPEKADDSLSYLTYQAGLVYDGGEEIVISPKNISNPDFSKMAFTYFITQDDF
                     RKLGNDLPELAANQVVFMRPSEKPSLKKLVLGDQSFENVANLDKAIFPDITNTLNAAV
                     MVVSDDTVLNAVREYYESNNPQGYLVSLDYRVFTDLTDEEVLSLGEQSGEIYEILDEN
                     GEYLGNVMQRTDFNNLLMGFTGGFLFTGFLLGISFLLGAALIIYYKQYSEGHEDKKSY
                     KILQEVGMSQKAVKKTINSQTLTVFFMPLVMATLHFVIALIMLKQMLLSFGVTSSLMI
                     YTVSGITLLAVTLIYFVIYKWTSRTYYRIIER"
     gene            2895..3146
                     /gene="relB"
                     /locus_tag="AC1PIW_001462"
     CDS             2895..3146
                     /gene="relB"
                     /locus_tag="AC1PIW_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HIP6606389.1"
                     /translation="MAAITLKVSEQDKLFMQAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADIALSEYEADLAKGIEPISWEEMLEELGLTDEDLL"
     gene            3130..3387
                     /locus_tag="AC1PIW_001463"
     CDS             3130..3387
                     /locus_tag="AC1PIW_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE family
                     toxin"
                     /protein_id="HIP6606390.1"
                     /translation="MKTCYKLVPTSRFIKQLKKLDKFTQKQITNYLSSHVTDNPRQYG
                     KALTANRSGQWRYRIGNYRVIVNIEDDKLIVTSIKMRNGSI"
     gene            3426..4091
                     /locus_tag="AC1PIW_001464"
     CDS             3426..4091
                     /locus_tag="AC1PIW_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIP6606391.1"
                     /translation="MKKEKIYIVEDDEMIVQLLKQHLGKSYQVESVQNFRAVSQEVAE
                     IKPDLVLMDINLPYYNGFYWTTEIRKTMTMPIIFISSSDDEMNAVMAMNMGGDDFVSK
                     PFSLGILDAKIGAFLRRVNQFSKSSELSIDQFQLSLDGRFSNESEQVQLSPTETKILS
                     ALLERQGQIVSKDELLEKLWENEEFIDQNTLNVNMTRLRKKVSEVGFERIHTVRGVGY
                     LIK"
     gene            4088..5029
                     /locus_tag="AC1PIW_001465"
     CDS             4088..5029
                     /locus_tag="AC1PIW_001465"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIP6606392.1"
                     /translation="MIAKFIREYRSFYFSYIVLATSFILLFFLYQLPTEFLQNALLLS
                     LTFLILLTVGLFWKFRNRMRALEDYVHEEALPLLNKPVDLAYLNLIEAEKEATREQLL
                     VYKSREEDLQAMVKMWSHQMKVPLAALSLMSQTDNLNQQDVNHQLLRLDNYMANLLNY
                     LKLTNHVSDFRFEQVEVREVVVDLVKKYRNQFLQKDISVIIDGNWLLKTDRKWLSFAL
                     SQIIDNALKYNKTGGKVAIELNGGISIFDTGIGILAEDLPRLFDEGFTGFNGREHQKA
                     TGFGLYLTKRVLNQLELAISVESQLEIGTTVSITKVQ"
     gene            5105..6613
                     /locus_tag="AC1PIW_001466"
     CDS             5105..6613
                     /locus_tag="AC1PIW_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HIP6606393.1"
                     /translation="MSHHHVLQNGSDIRGIAIATDEYAVNLTPQATQEVVRGLIHWLT
                     QKPELAQVYQKGQLTIGIGRDSRLSGPDLVAAFTEEAVCLGVQLIDFGMATTPALFMS
                     TQYPQFKCHAGVMITASHLPYYFNGIKIFSENGGAEHEDIDFILSHSEDLPASSLGSI
                     TSADLITPYAQDLVGKIRTACGGQEKPLTGLNIIVDAGNGAGGFFAEKVLAELGADTT
                     GSQFLDPDGTFPNHVPNPDNKEAMESIRQAVLKQGADLGIIFDTDVDRAALVTKSGEI
                     LNRNNLIAVLSQIVLAEHPGTSIVTNSPTTEHLKVFIESLGGKQIRYISGYRNVINRA
                     ILANQEGVDCQLAIETSGHAAFKENYFLDDGTYVAAKILMLLPKLQAEGKSLDDLIAQ
                     LKQPLETQEVRFKLEATDYRALGEQVIADLRQTSLEGFAFNPENEEGVRFDLTEPYGD
                     GWLLLRMSLHEPLLVLQVENDQTGHIPAVLRTLSAFLDQYPAVNQEKLKALI"
     gene            complement(6683..7753)
                     /gene="xerS"
                     /locus_tag="AC1PIW_001467"
     CDS             complement(6683..7753)
                     /gene="xerS"
                     /locus_tag="AC1PIW_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HIP6606394.1"
                     /translation="MRRELLLEKIDQLKEIMPWFVLEYYQSKLAVPYSFTTLYEYLKE
                     YRRFFEWLQDSDLVAVERIADIPLDVLEHLTKKDMEAFILYLRERPLLNANTTQNGVS
                     QTTINRTLSALSSLFKYLTEEVENEQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETMEFLDYVDKEYQVNLSKRALSSFQKNKERDLAILALLLASGVRLSEAVNLD
                     LRDVNLNMMIIEVTRKGGKRDSVNVAGFAKPYLEAYMGIRQQRYKAEKTDTAFFLSEY
                     RGLPNRIDASSIEKMVAKYSADFKIRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAN
                     TQVTDLYTHIVNDEQKNALDQL"
BASE COUNT         2156 a         1665 c         1759 g         2192 t
ORIGIN      
        1 ttcatgggat ttagtaccaa agaaaattag ctatactaaa tcaagttaat tttgctaaag
       61 aaaacaatta ggagtaacta tgttattaga aatcaatcat ttagaaaaag tttaccgcac
      121 gcgcttttca aaagaggaaa ctcgtgcctt gcaagatgtg gattttaagg tagaagaggg
      181 cgagtttatc gctatcatgg gggaaagtgg ttctggtaag acgactttgc tcaacattct
      241 agcaaccctg gacaagccga cgaggggctc tgttatctta aacaatcaag acatcaccaa
      301 aatcaaggaa agcaagctgg cagatttccg tttgcgtaat cttggctttg tcttccaaga
      361 ttttaacctc ttggacaccc tgtccatcca agacaatatc tttttacctc tggtattggc
      421 acgtgagagc tatcaggaaa tggagaaacg gttggaagta ttggcaccaa aactacggat
      481 tcaagatttg ctgaaaaaac gtccctttga actttctggt ggacaaaagc agcgcgtggc
      541 tattgctcgt agtttgatta cccagccaca gattgtccta gcggacgaac cgacggctgc
      601 cctagattat cgcaattcgg aagacttgct gaatttgttt gaagagatta accgtgacgg
      661 gcaaactatt ctcatggtga cccactcggc caacgcagcc agccacgcca aacgtgtctt
      721 gtttatcaaa gatggtcgta ttttccatca actgtacaag gcagacaaga gcaatcaaga
      781 ctttgccaag gagatttcac tcaatatgtc tgcactttta gggggtgagt agatgtttta
      841 cctcaaactt gctgggcaaa atatccgaaa atccatgggt gtttttgctc ccttcgtttt
      901 agccagtctg gtcctcttta ccctgatttg ttccatgttc ttgatcatgc tgagtccagt
      961 tatgaagacc atgggctctg gtgccatttc tatcggtctt ggtgtgatta ttctgaccat
     1021 tttctcggtc attatggaga tttatagttt taatttcctg atgaaacaac gggcgagaga
     1081 atttggtctc tataacatgc tggggatgaa caagaaaaaa gtagccttga tagctagcct
     1141 cgaattgatg atgattttct tgctagttgt ggttcttggt tctgttttag ctggtgtgtt
     1201 tgcaaatgtt ctctacttga tttttgtcaa tataacacat tatagtgatt tacattttgg
     1261 aattgagcct ctggcctttg tcatgactgc ctttctcttt gctggtttct tctttctctt
     1321 ggaattgcta gccattcgga ccattggcaa gaccagtccg ctgattttgt tccgagctag
     1381 tgaaaaagga gagagagagc cgaaaggaaa tgttcttcta gctgctcttg gtgtttttag
     1441 tcttgggatg ggctactacc tatcgctttc atcgaaagca gaaggtctag ctgttatcta
     1501 tcggttcttt atcgctgtgc tctttgtcat tgcggggacc tatctgttct acatcagttt
     1561 tatgacttgg tatttgaagg ctcgtcggaa aaacaagcgg tatttctata cgccagagca
     1621 tttcattacg acatcgcaga tgatttttcg gatgaagcag cacgcaacgg gtttggccaa
     1681 tattacgcta cttgcggtca tggcctttgt gaccattgcg accacaacct ccctttatac
     1741 aggtatgagc agtatgacag gtgcactata tcccaaggaa acatctatta cctataaagt
     1801 ttcggataga agtcaaggtg aaatggcctt ccagcaatcg gtgctcagcc attttccaga
     1861 aaaagccgat gacagtctat cctatctgac ctaccaagct ggtctggtat atgatggtgg
     1921 cgaagagatt gtcattagcc caaaaaatat ttctaatcca gatttttcta aaatggcctt
     1981 tacctacttc atcacccaag atgatttcag aaaattgggg aatgacctgc cagagcttgc
     2041 tgccaatcaa gtggtcttta tgcgtccgag cgaaaaacca tcgctgaaga agcttgtttt
     2101 gggcgatcaa tcctttgaga atgtagccaa tcttgataag gctattttcc cagacatcac
     2161 caatacgcta aatgccgccg ttatggtagt cagtgatgac acagtgttga atgctgttcg
     2221 tgagtattac gaatccaata atccgcaagg ctatcttgtt agcctagatt atcgtgtctt
     2281 taccgacttg actgatgaag aggtactctc tcttggagag caatctggtg aaatatacga
     2341 aattctcgat gaaaatggag aatatcttgg aaatgttatg caacgtacgg atttcaacaa
     2401 tttactgatg ggctttacag gcggtttcct ctttacaggt ttcttgctgg gaattagttt
     2461 cctactgggt gcagccttga ttatctacta taagcagtat tcagaagggc atgaggacaa
     2521 gaaatcctac aagattctac aagaagtagg catgagccag aaggctgtca agaaaacaat
     2581 taactcccaa acacttacgg tcttctttat gcctttggtc atggcgaccc tacactttgt
     2641 catcgccctt atcatgctca agcaaatgct actaagtttt ggtgttacct catcactaat
     2701 gatttacaca gtcagtggca tcaccctact ggcagtcact ctgatttact ttgtcattta
     2761 caagtggact agtcgcactt attatcgcat tattgaacgg tagcagaagt ctcgccttgt
     2821 gcgagatttc ttgctttttc agggaaatgg tgttacaatg gtaataccaa aggaatactc
     2881 gaagaggtga gaaaatggca gctattacat tgaaagtttc tgagcaggac aagctcttta
     2941 tgcaggctat ggcaaagttt gaaggtgtca gtttgtcaga actcattcgg actaaaaccc
     3001 ttgaagctct cgaagatgaa tatgatgccc gtgtggcaga tatcgctctg tctgaatacg
     3061 aggcagactt ggcgaaaggc attgagccga ttagctggga ggaaatgttg gaagagttgg
     3121 ggttgacgga tgaagacctg ttataaactc gtaccgacca gtcgttttat taaacagttg
     3181 aaaaagttgg acaaatttac ccaaaaacag attaccaact acttgtccag tcatgtgact
     3241 gacaacccta gacaatacgg aaaagccttg actgctaatc gttctggcca atggcgctat
     3301 cgtatcggca attacagggt tatcgttaat attgaagatg ataaattgat tgtgacaagc
     3361 ataaaaatga gaaatggaag tatataagcc aagacctgtg tcctggcttt tgcagaatga
     3421 agaagatgaa aaaagagaag atttatattg ttgaagatga tgagatgatt gtccaactct
     3481 tgaagcagca tttgggaaaa tcttatcagg tagaaagtgt gcagaatttc cgagctgtca
     3541 gtcaggaagt tgcggaaatc aaaccagact tagtgctgat ggacattaac ttgccctatt
     3601 acaacggttt ttattggacg acagaaatcc gtaaaaccat gaccatgccc attatcttta
     3661 tctcttctag cgatgatgag atgaatgcgg tcatggccat gaacatgggt ggcgatgatt
     3721 ttgtctccaa gccattttct ctaggaattt tggatgccaa aatcggagcc ttcctgcgtc
     3781 gggttaatca attcagcaaa tcgagcgaat tgtctatcga tcagttccag cttagtttgg
     3841 acgggcgatt ttcaaacgaa tcggaacagg ttcagctttc acctactgaa accaagattt
     3901 tatccgcctt actagaaaga caagggcaga ttgtcagcaa ggatgaactc ttggaaaaac
     3961 tctgggaaaa tgaagaattt atcgaccaga atacgctcaa tgtaaacatg accagactgc
     4021 gtaagaaggt gagcgaagtc gggtttgaac gaattcatac cgttcgtggc gtggggtact
     4081 tgatcaaatg atagcaaaat ttatccgcga ataccggagt ttttacttct cctacattgt
     4141 tctggctact agttttatct tgctcttctt tctctatcaa ctgcccaccg aattcctgca
     4201 aaacgctctg ctattgtccc taaccttctt aatccttcta actgtagggc ttttctggaa
     4261 gtttcgcaac cgcatgagag ccttggaaga ctatgtccat gaagaagctc tgcccctgct
     4321 taataagcct gtggacttgg cctatctcaa cttgattgag gcagagaagg aggcgacacg
     4381 ggagcagctt ttggtctata agtcacgaga agaagacctg caagctatgg tcaaaatgtg
     4441 gtcccaccaa atgaaagtgc ctttggctgc cctgtctttg atgagccaga ctgacaatct
     4501 caaccagcag gatgtcaatc accagctctt acgcttggat aattacatgg ccaatctcct
     4561 caactacctc aagctgacca accatgtcag tgattttcgt tttgagcagg tggaagtaag
     4621 agaagtggtg gtggatttgg tcaaaaaata ccgcaatcag ttcttgcaaa aggacatttc
     4681 agtgattatc gatggaaatt ggctactgaa aaccgacaga aagtggctta gttttgccct
     4741 atcacagatt atcgacaacg ccctcaagta caataagact ggtggaaagg tggccattga
     4801 attgaatgga ggaatttcca ttttcgatac gggaattggg attctggcag aagaccttcc
     4861 tcgcctgttt gacgagggat ttacaggttt taatggacga gaacaccaaa aagcgacagg
     4921 atttggattg tatctgacca agcgggtctt gaatcagttg gaactagcta tttccgtgga
     4981 aagtcaactg gaaattggga caactgtaag cattacaaaa gtacagtaga agctgtgctt
     5041 ttttcatttt gaaaccgctt cgaaaatatg atatactaaa tttagtttga attaggaggt
     5101 tactatgtcc catcatcacg tcttgcaaaa tggatcagat attcgtggaa ttgctatcgc
     5161 tacagacgaa tatgctgtca atctcactcc acaagccacc caggaagtag ttcgcggact
     5221 tattcattgg ctaactcaga agccggaact ggcgcaggtc tatcaaaaag gccaattgac
     5281 catcggtatc ggtcgagaca gccgcctcag cggtccagat ttggtcgcag cctttacaga
     5341 agaagccgtt tgtctgggcg ttcagctgat tgactttggc atggccacca cgccagctct
     5401 cttcatgagc acccagtatc cgcagttcaa gtgccacgct ggtgtcatga tcactgccag
     5461 ccacctgccc tattacttca acggcattaa gattttttcg gaaaatggcg gagcagaaca
     5521 tgaagatatt gacttcatcc tctcgcatag tgaagacttg ccagcttctt cccttggaag
     5581 tatcaccagt gcagacctga ttacgcctta tgcccaagat ctggttggta aaattcgtac
     5641 cgcctgtggt ggacaagaaa aaccactgac aggtcttaat atcatcgtcg atgctggaaa
     5701 tggtgcggga ggattctttg ctgagaaagt gttggcagaa ttgggagcag ataccactgg
     5761 ttcacaattc ctagacccag acggcacctt cccaaaccat gttccaaacc cagataacaa
     5821 ggaagctatg gaaagcattc gtcaggctgt cttgaagcaa ggtgcagacc taggcattat
     5881 ctttgatacc gacgttgacc gtgccgccct ggtcaccaag tcaggagaaa ttctcaatcg
     5941 taacaacctg attgccgttc tcagccagat tgtcctagct gaacatccag gaaccagcat
     6001 tgtgaccaat tccccaacga ctgaacacct taaggtcttt atagaaagtc tgggaggcaa
     6061 acaaattcgc tatatttcag gctaccgtaa tgtcataaac cgtgctattt tagccaatca
     6121 agaaggagtt gattgccagt tggctatcga aacatctgga catgctgcct ttaaggaaaa
     6181 ttacttcctc gatgacggaa cgtatgtagc ggctaagatt ctcatgctct tacccaaact
     6241 gcaagcggaa ggcaagtcct tggacgactt gattgcccag ctcaagcaac ccttggaaac
     6301 ccaagaagtg cggttcaaac tggaagccac agactatcga gcgcttggtg aacaggtcat
     6361 tgctgacctc cgccaaacca gcctcgaagg ctttgctttt aacccagaaa acgaagaagg
     6421 agtgcggttt gacctgactg agccttatgg tgacggctgg ctcttgctcc gtatgagcct
     6481 ccacgagccg ctcttggtct tgcaggtgga gaatgaccag actggacaca tacctgccgt
     6541 tttacgaact ctctcagcct ttttggacca gtacccagca gtcaatcagg aaaaattgaa
     6601 agcattgatt tagatttaag agaaaaaggg gtaagttttc gcttactcct taatttattt
     6661 acataattta tattacgtga tatcacaact gatccagagc atttttctgt tcatcgttga
     6721 cgatatgggt gtagagatcg gtgacctggg tattggcatg gcccagctga tgactgacta
     6781 gaacttgcga cttggtggcg tcgtagagac gagttgccaa tgtgtgacgg agtttgtggg
     6841 gggttacgcg tatcttgaag tccgcagagt acttggcaac cattttttca atagaagaag
     6901 catcgatacg gttaggcaga ccacggtatt cggacaggaa gaaggctgta tccgtttttt
     6961 cagccttgta gcgttgctga cggatgccca tgtaggcttc taagtagggc ttagcaaacc
     7021 cagccacatt gaccgagtcc cgtttaccac ccttacgagt tacttcgata atcatcatat
     7081 tgaggtttac atctcgaaga tccaaattca ccgcttctga cagacggacg ccagaagcca
     7141 agagaagcgc cagaatcgcc aaatcccgct ccttattttt ctggaaggag gagagggcac
     7201 gtttagagag attgacttgg tattccttgt ccacatagtc caagaactcc atcgtttcat
     7261 cgcccaaaaa gagcttttgc ttgatgttct ccgcccgcgc tgccaaggtt tccttcttct
     7321 tcttagtaga tacctttttc atgacattgc ggtagaagta gggctcgccc tgctcattct
     7381 ccacttcttc ggttaaatac ttgaagagac tagaaagggc cgagagggta cggttaatgg
     7441 tggtctgcga cacaccattc tgcgtggtat tggcgttcag caagggacgc tcccgcagat
     7501 aaagaatgaa agcttccata tctttttttg tcagatgttc cagaacatcc agcggaatgt
     7561 cagcaatccg ttcaacagcc accaaatccg aatcctgtaa ccattcaaaa aaacgacggt
     7621 attctttcaa gtattcatat aaggtagtaa aactgtaagg cacagccaat tttgactgat
     7681 aatattccag aacaaaccaa ggcataattt cttttagttg atcaattttt tccaataata
     7741 actcgcgtct catcattttc tcctgatttt ta
//
LOCUS       DBNFCK010000089         7700 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.089, whole genome shotgun sequence.
ACCESSION   DBNFCK010000089 DBNFCK010000000
VERSION     DBNFCK010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7700
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.089"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..266
                     /locus_tag="AC1PIW_001468"
     CDS             <1..266
                     /locus_tag="AC1PIW_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606395.1"
                     /translation="KINELLSDSELKLSKALKFQLLEAMELCEKGSKISYLSYKIYPW
                     VLEELALNRIQSDKLKMFKRYLEQERWKYYFGSALGMAFTSIR"
     gene            complement(388..1464)
                     /locus_tag="AC1PIW_001469"
     CDS             complement(388..1464)
                     /locus_tag="AC1PIW_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606396.1"
                     /translation="MKKLLICIVLLSCLVFPFIGWKLYAYQHNTITLTSEADFYLIYP
                     GKVETFQLNGQEPQLLHTQKMNSQGYFGSGENYVIDNRYLVFANDQQKFINDNLISID
                     FNTGTVLRKPSQYSSYISGTDGQDFYTVGPYHILAQFDRNFDTIQTHVLNDDFIPSAP
                     VMVDQTSVYLTGFVKDFTQEGPEENVLIVFDKKDFSKQEVFKYDNTIATGEGLWVNGT
                     IYLPNLGKKTSEYTEIEQSSELLTFDTKTKTFSSVTLEHPSPSTLHALKDQKLLLIEH
                     QNGMFSPISFTIYNTQTGQESYHRLDELNPRPYYIDHVRQLDGNRLLLILAGKALIYD
                     WQAKKVLSQTILSEDYVSGVWVND"
     gene            complement(1576..2949)
                     /gene="mnmE"
                     /locus_tag="AC1PIW_001470"
     CDS             complement(1576..2949)
                     /gene="mnmE"
                     /locus_tag="AC1PIW_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HIP6606397.1"
                     /translation="MITKEFDTITAISTPLGEGAIGIVRLSGTDAFAIASTVFKGKDL
                     ATVPSHSLNYGHAIDPATGQVLDEVMIGAMRSPKTFTREDVIEINTHGGIAVTNEILQ
                     LLIRQGARMAEPGEFTKRAFLNGRVDLTQAEAVMDVIRAKTDKAMHNAVRQLDGSLSQ
                     LINDTRQEILNTLAQVEVNIDYPEYDDVEEATTELVREKTLQFQALLENLLRTARRGK
                     ILREGIATAIIGRPNVGKSSLLNNLLREEKAIVTDIAGTTRDVIEEYVNIKGVPLKLI
                     DTAGIRETDDIVEKIGVERSKKALEEADLILLVLNASEPLTEQDRNLLAISDMANRIV
                     LLNKTDLEEQIEVDQLPEDVIRISVLQNQNIDQIEEKINQLFFENAGLVEQDATYLSN
                     SRHISLIEQAVQSLHAVNDGLKVGMPVDLLQVDLTRCWQILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            3170..5494
                     /gene="hsdR"
                     /locus_tag="AC1PIW_001471"
     CDS             3170..5494
                     /gene="hsdR"
                     /locus_tag="AC1PIW_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HIP6606398.1"
                     /translation="MVVFIKSKKEMSEEDIKANFITPAILSKGWKNGEQIAYEEYFTD
                     GRIEVRGDKGRRKEGKKSDYALYYQFGQRIAIVEAKDNKHSIRAGLQQAIEYGEILDV
                     PFVYSSNGDGFVEHDRITRQERELSLEEFPTRDELFERLKAEKEMAPEVSKAITTPYY
                     TDAFSIKKPRYYQQIAINRTIEAVASGQNRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFKPFEKVMTKITSKLLNAPEKLNSFEIYLGLYQQLMGE
                     DGTETHYQKFDKNFFDLIVIDEAHRGSAKEDSNWRKVIEYFSSATQIGMTATPKETKE
                     VSNMEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPENGKVDAKGQLIED
                     RYYGRKDFDKTIIIDDRTRKVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMREAFVN
                     ENQDLVREDYRYVMQVTGDNPEGKAQLDNFMDVNSKFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPEKGKEFFTIIDFRNVTNLFADPGFDGEPAKILD
                     PNLKTGQDSTSFTVGDPIEKYRVADRKVDILNSTVQVLDENGKLVTESLTDYTRKNIL
                     GTYATLTDFITAWHSADKKKVILEELYKKGVYLDAIREAENISEQEIDDFDLLLKLAY
                     GQKSLTKTERIRNVKQSGYLFKYSEEARAVLEVLLDKYMDKGIGELESIETLKLPEFH
                     VYGTPSKIVNTYFGNRKHYLEVIKELEEKLFAVA"
     gene            5507..6970
                     /locus_tag="AC1PIW_001472"
     CDS             5507..6970
                     /locus_tag="AC1PIW_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HIP6606399.1"
                     /translation="MSITSFVKRIQDITRNDAGINGDAQRIEQMSWMLFLKIYDSREL
                     DWELEEEEYESIIPEELKWRNWAHAEKGEQVLTGDDLLEFVNNKLFKELKELEITPNM
                     PIRKSIVKSAFEDANNYMKNGVLLRQMINVIDEVDFNSPEDRHSFNDIYEKILKDIQS
                     AGNSGEFYTPRAATDFIAEMLNPQLGETMADLACGTGGFLTSTLNHLGQQRKTSEDVQ
                     KYNQAVFGIEKKAFPHLLAVTNLFLHEIDDPKIIHGNTLEKNVRDYTEDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVEEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPEGYKN
                     FSKTKPMRNDHFNPVREWWKNRQEILEGNFYKSKSFRPDELASLNYNFDQCGFPKEEE
                     EILEPLELIQNYQQERAVLNKKIDTVLAEILEILEEK"
     gene            6970..>7700
                     /locus_tag="AC1PIW_001473"
     CDS             6970..>7700
                     /locus_tag="AC1PIW_001473"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HIP6606400.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQDPNDEPASELLKRIKEEKEKLI
                     SEGKVKRDKKETEFFRSDDGKPYEKLADGTVKKVEVPYEIPDSWEWVRLRNLGVITSG
                     GTPKSSESTYYDGNITWITPADMGKQQNNKLFATSSKKITELGVQKSSAQLISKNSIV
                     YSSRAPIGHINIVNYDFTTNQGCKSVTPILVNLDFMYWILQFRTKDIILRSSGTTFKE
                     ISASGFGDTLLPLPPLAEQKRIVAH"
BASE COUNT         2444 a         1261 c         1709 g         2286 t
ORIGIN      
        1 aaaaaataaa tgaactatta tctgattcag aattaaagtt gagcaaagca ttgaaattcc
       61 agttgttgga agcaatggag ttgtgtgaaa aaggtagtaa gattagctat ctttcgtata
      121 aaatttatcc atgggtattg gaagaactag ctttgaatag gattcaatcg gataaattaa
      181 agatgtttaa gcgctattta gagcaagaaa ggtggaaata ttattttggt tctgctttgg
      241 gaatggcgtt tacttcgatt agatagtggt tcctaggatt tataagtaca ttgattgtgc
      301 tgtgcaactt cgatagaata tgcgaaagtc ccaagaaaga gtcatcttgg gacttttaca
      361 atctttattg agattcgttt ttgtttatca gtcatttacc cacaccccgc taacataatc
      421 ctcagataga atagtttggc ttaaaacttt ttttgcttgc cagtcataga tgagtgcctt
      481 accagccaga atcaataata atctgttacc gtctagttgg cggacatggt caatataata
      541 aggacgagga ttaagctcgt ctagacgatg gtagctctct tgacctgttt gggtattata
      601 aatggtaaat gagatcgggc taaacatacc attttgatgt tcgatgagga gaagcttctg
      661 gtctttgaga gcatgtagtg tgcttggtga ggggtgttct aaagttactg acgaaaatgt
      721 ttttgttttt gtatcaaagg ttaggagttc ggatgactgt tcaatttccg tatactcact
      781 tgtttttttc cctaaatttg ggagatagat ggttccattg acccaaagcc cttctcctgt
      841 ggcaatggta ttgtcgtact tgaatacctc ttgttttgaa aaatctttct tatcaaatac
      901 aatcaagaca ttttcttcgg gtccttcttg ggtaaaatct ttcacaaaac cagttaaata
      961 gactgacgtt tggtctacca tgactggagc acttgggatg aagtcatcgt ttaaaacgtg
     1021 ggtttgaata gtatcaaaat tcctgtcaaa ttgcgctagt atgtgatagg gccccactgt
     1081 ataaaagtct tgtccatctg taccactgat atagctacta tactgacttg gctttcgcaa
     1141 gacagtgcca gtattgaaat cgatggatat cagattgtcg ttaatgaatt tttgctgatc
     1201 gttggcaaag actagatacc gattgtctat gacataattt tcaccagagc caaaataccc
     1261 ttgagaattc attttctgtg tatgaaggag ttgaggctct tgcccattta gctgaaaagt
     1321 ctcgaccttg ccaggataaa tcaagtaaaa atcagcttcc gacgttaatg tgatcgtatt
     1381 gtgctgatag gcatatagtt tccagccgat aaatggaaag acaagacagg atagaaggac
     1441 aatacaaata agaagttttt tcatgggaat gccgcctttc actgtttagt atatctaaat
     1501 ttgtcatctt agacaagaaa ttatgtgtta atatacaaat aaagtcatgc aagttttctt
     1561 acatgacttt tgttcttatt ttccaagaca gaattggcta aagagttggg taatgagttc
     1621 atctggagca gcatcacctg ttatttctcc caaaatttgc caacagcgtg tcaggtcaac
     1681 ctgtaacaag tctactggca tgcccacttt cagtccgtca ttgacagcat gtaggctttg
     1741 aacggcttgc tcaatgaggg aaatatgacg agaattggag aggtaggtgg catcttgctc
     1801 taccagacca gcattttcaa agaagagttg attgattttt tcttcaatct gatcgatgtt
     1861 ctggttttgc aagacggaaa tgcgaatgac atcctcaggc agttggtcta cttcaatctg
     1921 ctcttccagg tcggtcttgt tgagcaggac gatgcggttg gccatatcgg aaatggctag
     1981 gagattgcgg tcttgctcgg tcaggggctc agatgcgttg aggactagca ggatcaggtc
     2041 ggcctcctca agagcttttt tggaccgttc cacaccaatt ttttcaacga tgtcgtctgt
     2101 ttcacggata cctgctgtat caatcagctt gagggggacg cccttgatgt tgacgtattc
     2161 ttcgataacg tcacgggttg ttcctgcgat gtctgtaacg atggcttttt cctcgcggag
     2221 gaggttattg agcaggctgg attttcccac atttggacga ccgataatag cggttgcgat
     2281 gccttcacgc aggatttttc cacggcgggc agtgcggagg agattttcca aaagagcttg
     2341 gaactggagg gtcttctcac ggaccagctc tgtcgtcgcc tcctcgacgt cgtcgtactc
     2401 ggggtagtcg atgttgacct cgacctgagc cagtgtgttg agaatctcct ggcgcgtgtc
     2461 gttgatgagc tgggagaggg agccgtcaag ctggcggacg gcattatgca tggccttgtc
     2521 ggtcttagcg cggataacat ccatgacggc ttcggcctgg gtcaaatcca cacgaccatt
     2581 gagaaaggcc cgcttggtaa attcacccgg ctcagccatt cgagcccctt ggcgaatgag
     2641 gagctggagg atttcgttgg taacggcgat gcctccgtgg gtgttaatct cgatgacatc
     2701 ttcgcgggtg aaggtctttg gagaacgcat ggctccaatc ataacctcgt ccagcacttg
     2761 acccgttgca ggatcaatgg catgaccgta gttgaggctg tggctgggta cagtcgccaa
     2821 gtccttgccc ttgaaaaccg tgctggcaat ggcaaaagcg tccgtcccag ataggcggac
     2881 aatcccgata gcgccctcac cgagaggggt ggaaatagcg gtaatggtgt cgaattcttt
     2941 ggtaatcata tatctttctt tctaaaattg tcttatctat tagtgtatca tggaatgatg
     3001 aaaaaagcac agatgaggga tggggatgta aatatattta tgtttacttt gagaattact
     3061 ttattataat attgtagttt gattatttag gtgtggtata atagaaatat agtttgttct
     3121 gataatctta ttatcctgaa gttattattc atactgaaag aggaaaatta tggttgtatt
     3181 tatcaaatct aaaaaagaga tgagtgaaga agacattaaa gcaaacttta ttacaccagc
     3241 tattttgtca aaaggctgga aaaatggaga acagattgct tatgaagaat atttcacaga
     3301 tggtcgaata gaagttcgag gtgataaagg acgtcgaaaa gagggaaaaa aatcagatta
     3361 cgctctctat tatcaatttg gacagcgaat tgcaatcgtt gaagctaagg ataataagca
     3421 tagtattcgt gcaggtttac aacaagcgat tgaatatggt gaaattttgg atgttccttt
     3481 cgtttattcc tcaaatggtg atgggtttgt tgagcatgac cgaattacta gacaagagag
     3541 ggaactctct ttagaagagt ttccaacccg tgatgaatta tttgagcgat taaaggctga
     3601 aaaagaaatg gcaccagagg tttcgaaagc aataacaact ccttactata cagatgcttt
     3661 ttcgattaag aaaccacgct attatcagca aattgcaatt aatcgtacaa ttgaggcagt
     3721 tgcaagcggg caaaacagag tcatgtttgt aatggcaaca ggtactggaa aaacctttat
     3781 ggcttttcaa attattcacc gacttaggaa agccggatta gcaaagcgtg tcttgttttt
     3841 agcagacagg aatattttag ttgaccaaac aatggcagaa gatttcaagc catttgaaaa
     3901 agtgatgact aaaatcacat caaaattgct gaatgcgcca gaaaaattaa attcttttga
     3961 aatatactta ggtctttatc agcaattaat gggagaagat ggaaccgaaa ctcactatca
     4021 aaaatttgat aaaaatttct ttgatttgat agtgattgat gaagctcata gaggttcagc
     4081 aaaagaagat agtaattggc gtaaagtgat tgagtatttt tcatcggcaa cgcaaattgg
     4141 tatgactgcg acaccaaaag aaactaaaga agtttccaat atggagtatt ttggtgagcc
     4201 aatttatact tatagcctga agcaagggat agaggatgga tttctcgctc catatcgagt
     4261 tatgcgagtc aatctagatg tggacgtaga tggctatcgt ccggaaaatg gaaaagttga
     4321 tgctaaggga cagcttatcg aagataggta ttatggtcgc aaggattttg ataagacaat
     4381 tatcattgat gatcgtacac gaaaggttgc aaaatttgtt tcagattata tgaaacaaaa
     4441 taatgcacgc tttgacaaaa caattgtatt ttgtgttgat attgatcatg ccgaacgtat
     4501 gcgtgaagca tttgtcaacg aaaatcagga tttggtacgg gaggactatc gttatgttat
     4561 gcaggtgaca ggtgacaatc ctgaaggaaa agcccaacta gataatttta tggatgttaa
     4621 ctctaagttt ccggcgattg tgacaacttc taaactttta accacaggtg ttaatgcaaa
     4681 aacttgtcga ttgattgtat tagattcaaa tattcaatca atgacagaat ttaaacagat
     4741 tattggtcgt ggaacacgtt tatatcctga aaaagggaaa gagtttttca ctattattga
     4801 ttttaggaat gtgacaaatc tatttgctga tcctggattt gatggtgagc cggcgaaaat
     4861 attagatcct aatctgaaaa ctggacagga ttcaacatca tttacagtag gtgatcctat
     4921 agaaaaatat cgagttgcgg ataggaaagt ggatattctc aattcgacag ttcaagtctt
     4981 ggatgaaaat gggaaattag taactgagag tctaacagat tatacacgta aaaatatttt
     5041 aggaacatat gcgacattaa ccgattttat tactgcatgg cattcagcag ataagaaaaa
     5101 agttatttta gaagaactct ataaaaaagg tgtatatctg gatgcaattc gtgaagcaga
     5161 aaatatttcg gagcaagaaa tcgatgattt tgatttactt ttgaaactgg catacgggca
     5221 gaaaagtttg actaaaaccg agcgaatcag aaatgtgaaa caaagtggat atctatttaa
     5281 atacagcgaa gaagcgcgtg ctgtattaga ggttttacta gataagtata tggataaggg
     5341 aatcggtgag cttgaaagta tcgaaacttt aaagttacca gaatttcatg tttatggtac
     5401 tccttccaag attgttaata cctattttgg aaatagaaaa cattacttag aagtaattaa
     5461 agaattagaa gaaaagctat ttgctgtagc ttaatgagag gaaaaaatgt caattacatc
     5521 atttgttaaa cgaatacaag atattactcg aaacgatgca gggataaatg gtgatgccca
     5581 gagaattgaa caaatgtctt ggatgctatt tttaaaaatc tatgacagtc gagagttaga
     5641 ttgggagttg gaggaagaag aatacgagtc aattattccc gaggagttga agtggcgaaa
     5701 ctgggctcat gctgagaagg gtgagcaggt cttgactgga gatgacttac ttgagtttgt
     5761 gaataataaa ttgttcaaag agttgaaaga actcgaaatc actccaaaca tgccaattcg
     5821 caaatcaatt gtaaaatctg cttttgaaga tgctaacaac tatatgaaaa atggagttct
     5881 tctgcggcaa atgattaatg tgattgacga agttgatttt aacagtccag aagatcgtca
     5941 ctcatttaat gatatttatg agaagattct caaagatatt caaagtgcag gaaattctgg
     6001 tgaattttac acaccacgtg cagcaacaga ttttattgct gaaatgctca atcctcaatt
     6061 aggggaaacg atggcagatt tagcatgtgg gacaggtggt ttcttgacat ctaccttaaa
     6121 tcatttaggc caacagcgaa agactagcga agacgttcaa aaatacaatc aagcagtttt
     6181 tggtattgag aaaaaggctt tcccccacct attagcggtg acaaatttat tcttgcatga
     6241 aattgatgat ccgaaaatca tccacgggaa tacgttggaa aaaaatgtga gggattacac
     6301 tgaggatgaa aagttcgata tcatcatgat gaatcctcca tttggtggtt ctgaacttga
     6361 aacaataaaa aataattttc cagcagagtt acgcagctct gaaacagcag atttgtttat
     6421 ggctgttatt atgtatcgac ttaaagaaaa tggtcgtgtt ggtgtaattc taccagatgg
     6481 attcctcttt ggtgaaggag taaaaacacg actaaagcag aaattggttg aggagtttaa
     6541 tcttcacact attattcgct taccacatag cgtatttgct ccatatacag gaattcatac
     6601 gaatattctc ttttttgata agacgaaaaa aacagaagaa acttggtttt acagattgga
     6661 tatgccagaa ggctataaaa atttctctaa aactaaacca atgagaaacg atcactttaa
     6721 tcctgttcgc gaatggtgga aaaatcgaca agagatttta gaagggaatt tctacaaatc
     6781 taaatcgttt agaccagatg agttagctag tttaaactat aattttgatc aatgcggttt
     6841 tccaaaggaa gaggaagaaa ttttagagcc acttgaactt atccaaaact atcagcaaga
     6901 acgagctgtc ctaaataaaa aaattgatac tgttttggcg gaaattttag aaattttgga
     6961 ggagaaataa tgactcctga acaactgaaa gcaagtatcc tgcaaagagc gatggagggt
     7021 aagcttgttc cgcaagaccc aaatgatgag cctgcaagtg aacttttgaa acgtatcaag
     7081 gaagagaaag agaagttgat ttcggaagga aaagtcaagc gagataaaaa ggaaacagaa
     7141 ttttttcgta gtgatgatgg gaaaccttat gagaagttag ctgatgggac tgttaaaaaa
     7201 gttgaagtac cttatgaaat tccagatagt tgggagtggg tgaggttaag aaatttagga
     7261 gttattactt cgggaggcac accgaaatct tctgaatcta cttattatga tgggaatatc
     7321 acttggataa ctcctgcaga tatgggtaaa cagcaaaaca ataaattatt tgccacctca
     7381 tcaaaaaaaa ttaccgaatt aggcgtacaa aaatcttctg ctcaactaat ttcaaaaaac
     7441 tctattgttt attctagtcg agctccaata ggacatataa acattgttaa ctatgacttt
     7501 actacaaatc aaggttgtaa atccgtaacg ccaatactag taaatttaga ttttatgtat
     7561 tggattttgc aatttcgaac aaaagatatt attctacgat catcaggtac gacatttaag
     7621 gagatttctg cttcaggttt tggagataca cttctccccc ttcccccgct tgccgaacaa
     7681 aaacggattg ttgctcacat
//
LOCUS       DBNFCK010000090         7690 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.090, whole genome shotgun sequence.
ACCESSION   DBNFCK010000090 DBNFCK010000000
VERSION     DBNFCK010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7690
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.090"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..599)
                     /locus_tag="AC1PIW_001474"
     CDS             complement(<1..599)
                     /locus_tag="AC1PIW_001474"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIP6606401.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDFMRTRQAQNTGFLKELEEFA
                     HQENIPVIPHETVAYFRLLMQTLQPKRILEVGTAIGFSALLMADNSPHSKITTIDRNE
                     EMIGFAKENFAKYDYRQQIELLEGEAMDILPTLPDNTYDFIFMDSAKSKYIVFLPEVL
                     KKVKVGGLIILDDIFQGGDVAKDIKDIRRGQRTIYKGLQR"
     gene            complement(674..1222)
                     /locus_tag="AC1PIW_001475"
     CDS             complement(674..1222)
                     /locus_tag="AC1PIW_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6630 family protein"
                     /protein_id="HIP6606402.1"
                     /translation="MLTEEQLQDIFELADLMSNGDRELFLQLREVVFASDPHEILNFM
                     ERILDSESFDDFLDRVGESEKENLWLILIKLLEHLNYICVRDYKDNLEDFIYFFDCLQ
                     QVRNAGISLKLDSGGLSPIASISEWARVIDNKYLDENFCLGAVDMDTDSYYLFFSKQA
                     TFIRLQELAGNLGYRIDYAKNM"
     gene            complement(1238..1678)
                     /locus_tag="AC1PIW_001476"
     CDS             complement(1238..1678)
                     /locus_tag="AC1PIW_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606403.1"
                     /translation="MISYKRSIVIPSVISLVLILLGLGGLTKQEPSMGSVVYLIFLQA
                     LLVYLYILRKQKAVFILTKQYLEFQEHFWSERQRHSLEEIAEIRYMVSPVYRGYQSKR
                     LRIVGNKGGLLAVVSLNRLDGADFNDIYHFVEQVAPHIRWDFSN"
     gene            complement(1680..3482)
                     /gene="pepF"
                     /locus_tag="AC1PIW_001477"
     CDS             complement(1680..3482)
                     /gene="pepF"
                     /locus_tag="AC1PIW_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HIP6606404.1"
                     /translation="MSKQRYEIEEKYQWDLTTIFPTDEAWEAELADLQAETEKTKEFA
                     GHLLDSAKSLLEISETQLSLMRRIEKLYVYASMKNDQDTREGKYQEFQAKAMGLYAVF
                     SQAFAFYEPEFMGITEEKLEAFKVEEPALVQYSHQFEKLLAAKDHVLSQEVEEVLAAT
                     SEIFESPAETFSVLDNASLRFPEITDEDGHLVPLSHGNYIHFMESKNRDVRQEAYEAM
                     YGTYEQFQHTYAKTLQSNVKVNNLKARLRKYDSARHASLAANFIPESVYDTLVAAVNK
                     HLPLLHRYINLRKKLLGIDDLKMYDMYTPLSDFSTKISYEDALKKCQETLEIFGEDYS
                     AIVKEAFENRWIDVHVNEGKRSGAYSGGAYDTNAFMLLNWQDNLDNMYTLIHETGHSL
                     HSMLTRKTQPYVYGHYSLFLAEIASTTNENLLTEKLLAEVEDDKERFAILNHYLDGFR
                     GTVFRQTQFAEFEQAIYKADQEGQVLTADFLNELYGQTNEKYYGLSAEENPEIQFEWA
                     RIPHFYYNFYVYQYATGFAAASALANKIVNGSPEDKENYLNYLKAGSSDYSLNVIAKA
                     GVDMTKEDYLNDAFKVFEARLEELEALVEKGVHL"
     gene            complement(3713..4654)
                     /locus_tag="AC1PIW_001478"
     CDS             complement(3713..4654)
                     /locus_tag="AC1PIW_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HIP6606405.1"
                     /translation="MLVALDEDGQVFNVLENPAPQGSFSCPGCGGLVRYKSGKVLRSH
                     FAHVTLRDCHYFSENESAQHLSLKSCLYRWLVNAEQVELEKCLTSIGQVADLFVNNSL
                     ALEVQCSGLPISRLQLRTQAYSEAGYQVLWLLGKDLWLKERLTNLHKQFLSFSMNMGF
                     HLWELDDEKKELRLRYLIHEDLRGKVHCLTKVFPFGEGNLLDILRLPFAKQALSHLTC
                     PLDRDLPRYIAQQLYYKSPNWLALQAESYSRGENLLTKTAEEWYPHIRLPRSAIGFAQ
                     IQKDLTLVYQDFDQYYGNIEDKQKQVLYPPIIYRKPM"
     gene            complement(4718..6721)
                     /gene="metG"
                     /locus_tag="AC1PIW_001479"
     CDS             complement(4718..6721)
                     /gene="metG"
                     /locus_tag="AC1PIW_001479"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIP6606406.1"
                     /translation="MTKTPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGHEV
                     FYLTGLDEHGQKIEEKAKEAGLTPQAYVDGMAVGVKELWNLLDISYDKFIRTTDDYHE
                     EVVAAVFEKLLAQDDIYLGEYSGWYSVSDEEFFTESQLEEVFRDENGKVTGGIAPSGH
                     EVAWVSEESYFLRLSKYQDKLVEFFNAHPDFIQPDGRLNEIMKNFIEPGLEDLAVSRT
                     SFTWGVPVPSNPKHVVYVWIDALLNYATALGYGQEETVNYDKFWNGTVYHMVGKDILR
                     FHSIYWPIMLMMLDMKLPDRLVAHGWFVMKDGKMSKSKGNVVYPEMLVERFGLDPLRY
                     YLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVAMINKYFGGQVPTFVANVT
                     DFDADLAAVVADNLASYHKYMEAVDYPRALEAVWNIISRTNKYIDETAPWVLAKDEAD
                     RDKLAAVMAHLTAGLRVVAHLIQPFMMTTSNAIMEQLGLGTAFDLENLDFAGLPAGLT
                     VVAKGTPIFPRLDMEEEIAYIQAQMGGSSAISQEEEKEWNPADVELKNEKAAIKFEDF
                     DKVEIRVAEVKEVAKVKGSDKLLKFRLDAGDGQDRQILSGIAKYYPNEQELVGKKVQI
                     VANLKPRKMMGLLSQGMILSAEHGDNLTLLTVDPSVPNGSQIG"
     gene            6877..7470
                     /locus_tag="AC1PIW_001480"
     CDS             6877..7470
                     /locus_tag="AC1PIW_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HIP6606407.1"
                     /translation="MFTKKNILTHLFFLIIAVGLKRYGVTAPDLTWNMFLALVALDFA
                     ILTNHTKSHILKVIAGLLWLFFYPNTFYMVTDIVHMHFANTVLWQRESMILFMLYVPS
                     IFFGVMAGVESVRLIFSAFSIKSYWLKLFMISGLSLLSSFAIHIGRYARLNSWDIFTR
                     PTVVIDEMLAVISWDSLPFILGFTFIQIMVLVFSINE"
BASE COUNT         2173 a         1733 c         1483 g         2301 t
ORIGIN      
        1 cgttgcaatc ccttataaat cgttcgctgc cctcttctga tatccttgat gtccttggca
       61 acatctcctc cttggaagat gtcatccaaa atgattaaac cacctacttt gacctttttc
      121 aagacttctg gtaaaaatac aatatatttt gatttagcag aatccatgaa aataaaatca
      181 tatgtgttat ctggtaaagt aggaagaata tccatagctt ctccttccag caattcaatc
      241 tgctggcgat aatcatactt ggcaaagttt tccttagcaa atccaatcat ttcttcattt
      301 cgatcaatcg tcgtaatctt cgagtgcggg ctattgtcag ccatcagtaa cgcagagaag
      361 ccaatagcag ttccaacttc taaaatacgt ttaggttgga gtgtctgcat cagcaagcgg
      421 aaataagcta ccgtttcatg agggataact ggaatgtttt cttgatgagc aaactcttcc
      481 agttctttca aaaaccctgt attttgcgcc tgacgtgttc gcataaaatc cacaatttct
      541 tccttgacaa ccggacggcg catattgtga tttgcgtttt tactatatga ttcaaccata
      601 gaatccatta tatcataagc cgacaagatt ttcttcctca atacactaaa aggtaggttt
      661 ccccaccttt tctctacata tttttggcgt aatcaattcg ataccctaaa tttccagcta
      721 gttcttgaag tcgaataaaa gtcgcctgct tactgaaaaa gagatagtaa ctatccgtgt
      781 ccatatctac tgcccctaga caaaagtttt catcaagata tttgttatca atgactcttg
      841 cccattctga aatagatgca attggactta atccacctga atccaattta agagaaattc
      901 ccgcattgcg gacctgctga aggcaatcaa aaaagtagat aaaatcttca agattgtcct
      961 tataatcacg cacacaaata tagttcaaat gctctagtaa ttttatcaag attaaccaaa
     1021 gattttcctt ttcagactct ccaacacgat ccaagaaatc atcgaatgac tctgaatcta
     1081 aaatacgttc cataaaattg agaatctcgt gtggatcact ggcgaagact acttctctaa
     1141 gttggagaaa tagttcccta tcaccatttg acattaaatc tgctaattca aaaatatctt
     1201 gtagttgttc ttctgttaac atagtcccct ctctttatta atttgaaaaa tcccatctta
     1261 tatgcggtgc tacttgctct acaaaatggt aaatatcatt aaaatctgca ccatcaagcc
     1321 tattcaagct gacgactgca agtagtcccc ccttatttcc tacaattcgc aaacgtttcg
     1381 actgatagcc acgatagaca ggagaaacca tgtatcgaat ctctgcaatc tcttctaacg
     1441 aatggcgttg acgttcactc caaaagtgtt cttgaaattc taggtactgt tttgtcaata
     1501 taaaaactgc cttttgcttt ctcaatatat ataaataaac tagcaaagct tgtaaaaata
     1561 tcaaatagac aacacttccc atagatggct cttgcttggt caaaccacca agacccagca
     1621 agattaaaac aaggctaatc acactgggaa tgacaatcga acgtttatag gaaatcatat
     1681 tagaggtgca cacctttctc aacgagagct tctaattctt ccaaacgggc ttcaaatacc
     1741 ttgaaggcat cattgaggta atcttccttg gtcatgtcta cacctgcttt ggcaatgaca
     1801 ttgagggaat aatctgagct accagccttc aagtagttga gatagttttc tttgtcttct
     1861 ggactaccat tgacaatctt gtttgctaga gctgatgctg ctgcgaaacc tgtcgcatac
     1921 tgatagacat agaaattgta atagaagtgt ggaatacgag cccactcaaa ctggatttct
     1981 ggattttctt cggcagaaag tccgtaatat ttctcgtttg tctgaccgta caattcattg
     2041 aggaaatcag ccgtcaagac ctgaccttcc tggtctgcct tatagatagc ttgttcaaac
     2101 tcggcaaatt gggtctggcg gaagactgta ccacggaaac catctaggta atgatttaga
     2161 atagcaaagc gttccttgtc atcttctact tctgccaaga gtttttcagt cagaaggttt
     2221 tcgttggtag ttgaagcaat ttcagccaag aaaagtgaat aatggccata gacatagggt
     2281 tgagtcttac gagtcaacat ggaatgaaga gaatgtcctg tttcatggat aagggtgtac
     2341 atgttatcca agttatcctg ccagttgaga agcatgaagg cgttggtgtc gtaggcacct
     2401 cctgagtatg ctcctgagcg ttttccttca ttaacatgga catcaatcca gcggttttcg
     2461 aaggcttcct tcacaatagc cgaataatcc tcgccgaaga tttccaaggt ttcttgacat
     2521 tttttaagag catcttcata agaaatcttg gtgctgaaat ctgacaatgg tgtgtacata
     2581 tcgtacatct tcaagtcatc aatccccagg agcttcttac gcaaattgat gtaacgatgc
     2641 aagagcggaa ggtgcttgtt gactgccgcc actaatgtat cgtaaacaga ttctggaatg
     2701 aaattagctg caagtgatgc gtgacgggct gaatcgtact tccgcaaacg agctttcaag
     2761 ttattgacct tgacattaga ctgaagggtc ttagcgtatg tatgctggaa ctgctcataa
     2821 gtaccgtaca tagcttcata ggcttcttgg cggacatcac ggtttttaga ctccataaag
     2881 tggatgtagt ttccgtgtga aagtggcacc aagtgaccgt cttcatctgt gatttctggg
     2941 aaacgcaggc tggcattgtc caagactgag aaggtttcag ctggtgattc gaaaatttcg
     3001 ctggtcgccg ccaatacttc ttccacctct tgtgacaaga catggtcctt agctgctaaa
     3061 agtttttcaa attgatggct gtactgaaca agagccggct cttctacttt aaaggcttcc
     3121 aatttctctt cagtaatgcc cataaattca ggctcataga aggcaaaagc ctgtgaaaag
     3181 acagcataca agcccatggc cttagcttga aattcctgat attttccttc gcgagtatct
     3241 tggtcgttct tcatagacgc atagacatag agtttttcaa tgcgacgcat aagactaagt
     3301 tgcgtttcgc taatttctaa gagactttta gctgagtcca gtaaatgacc tgcaaattct
     3361 tttgtttttt cagtttcagc ttgtaaatct gcaagttctg cttcccaggc ttcatcggtt
     3421 gggaaaatag tggttaaatc ccactggtat ttttcttcaa tttcgtaacg ttgtttagac
     3481 ataattaatc ctccgtattc tttattctaa cataaaaaat cccctctttc caacggaaag
     3541 tgagggctta taggttactt atttcaactg gaaaacagtt tttgccattc gctatcgcaa
     3601 gtcgtcacta ggctaacaaa gtctacaacc ttcaccgaca gtagtatgac taacataaaa
     3661 tattgagcat attttcaaaa gtaatatata cacctccatc agctatctct acttacattg
     3721 gttttctata aattataggt ggatatagaa cctgtttctg cttgtcttca atattgccgt
     3781 aatactggtc aaaatcctga taaactaggg ttaaatcttt ttgtatttgg gcaaagccaa
     3841 tggcagagcg gggcagacgg atgtgggggt accattcttc tgccgtcttt gtcagcaaat
     3901 tctcccctcg gctataagac tctgcctgca gggccaacca attgggggat ttgtaataaa
     3961 gctgttgagc aatgtagcga ggcaaatccc tatctaacgg gcaagtaaga tgcgataaag
     4021 cttgcttggc aaatggcaga cgcaagatgt ccaataaatt tccctcgcca aatggaaaaa
     4081 cctttgtcag acagtggacc ttacctcgta agtcttcgtg aataaggtaa cgaagacgca
     4141 attccttctt ctcatcatcc aattcccaga ggtgaaaccc catattcata ctaaaagaga
     4201 gaaattgctt gtgcaaattg gtcagtcgtt ccttgagcca caaatccttg ccaagcaacc
     4261 aaagaacctg ataaccagcc tcactatagg cttgcgtcct cagctgcaaa cgagaaatgg
     4321 gcagaccgga acattgaact tccaaagcta gactattatt gacaaataaa tcagcgacct
     4381 gtcctatact agtcaagcat ttttcaagtt caacttgttc ggcattgact aaccacctgt
     4441 acagacaaga cttgagggac aggtgctgag cggactcatt ctcggaaaaa tagtggcagt
     4501 cccgcagggt tacatgggca aagtgcgaac gcagaacttt tcctgatttg tatcggacaa
     4561 gtcctccaca acctggacag gaaaaactgc cttgcggcgc aggattttcc aagacattaa
     4621 aaacttgccc atcttcatca agtgctacta acatattacc tcctttctat attagttcgt
     4681 aaaaaaacac ccagtttaac tgagtgtttg aaatttttta cccaatctga ctgccgtttg
     4741 gcacagatgg atcgactgtt aagagtgtta aattgtcacc atgttcggct gataggatca
     4801 tgccctgact gagcaagccc atcatcttac gtggcttaag gttggcaacg atttgaactt
     4861 tcttgccaac caactcttgc tcatttggat aatacttggc gataccagac aggatttggc
     4921 ggtcttgtcc atcaccagcg tccagacgga acttgagcaa cttgtcagaa ccctttactt
     4981 tggctacttc tttaacttcg gccacacgga tttccacctt gtcaaagtcc tcgaacttga
     5041 tcgcagcttt ttcgtttttg agttccacgt cagctgggtt ccactctttt tcttcctctt
     5101 gggaaatagc agagctgcca cccatttggg cttggatata ggcgatttcc tcttccatat
     5161 ccaaacgtgg gaagataggc gttccttttg cgaccactgt caaacctgct ggaagacctg
     5221 caaagtcaag attttcaagg tcaaatgctg ttcccaaacc aagctgttcc ataatggcat
     5281 ttgaagtggt catcatgaat ggctgaatga ggtgagccac cacacgaaga cctgctgtta
     5341 agtgagccat gactgctgcc aacttgtcac ggtccgcttc gtccttagcc aagacccaag
     5401 gtgcagtttc atcgatgtac ttgttggtac gggagatgat attccaaaca gcttccaagg
     5461 cgcgtgggta gtcaacagct tccatatact tgtggtagct tgccaagttg tcagctacaa
     5521 ctgccgccaa gtccgcatcg aagtcagtca cattggcaac aaaagtaggt acctgaccac
     5581 cgaaatactt gttaatcatg gcaacggtac ggttgagcag gtttccaagg tcgttggcta
     5641 gttcgtagtt gatacgacca acataatctt ctggtgtaaa ggtgccatca gagccgactg
     5701 gcagactacg catgaggtaa taacggagtg gatccaagcc gaaacgctca accaacattt
     5761 ctggatagac cacattgccc ttagacttgg acatcttgcc atctttcatg acaaaccagc
     5821 catgagcaac caagcggtct ggcaacttca tatccaacat catgagcatg attggccagt
     5881 aaatggagtg gaaacgaaga atgtccttgc caaccatgtg gtaaactgtt ccattccaga
     5941 acttgtcgta attaactgtt tcttcctgac cataacccaa ggcagttgcg tagttgagta
     6001 gggcatcaat ccagacatag acaacgtgct ttggattgga cggtacgggc acgccccatg
     6061 taaatgaagt acgtgacact gccaaatctt ccaagcctgg ctcgatgaag tttttcataa
     6121 tttcattgag gcggccgtct ggctggataa agtctggatg agcattgaaa aactcgacca
     6181 acttatcctg atacttgctg aggcgaagga agtaggattc ttctgaaacc caggctactt
     6241 cgtggccgct cggagcgata ccacctgtta ctttgccgtt ttcatctcgg aagacctctt
     6301 ctaattggct ctcggtgaaa aattcctcgt ccgatacgga gtaccaacca gaatactctc
     6361 ccaagtaaat gtcgtcctgc gctagcaatt tttcaaaaac agccgccacc acttcctcgt
     6421 ggtaatcatc ggtcgtgcgg ataaatttgt cgtaagagat gtccagcaag ttccagagct
     6481 ccttgacacc aaccgccata ccgtcaacgt aggcttgcgg agtcaaacca gcttcttttg
     6541 ccttctcttc aatcttctgg ccgtgctcat caagccctgt cagataaaaa acctcgtggc
     6601 ccatcaggcg tttgtaacga gccaatacat cgcaggcaat ggtcgtgtag gctgaaccga
     6661 tgtgaagttt tccagacgga taataaatcg gtgtggtaat gtaaaacggt gtttttgtca
     6721 tgtttttttc ctttcaaatt cagagcaaat gaaaccctgt tattgacaac atcctatttt
     6781 atcatatttt cagctaaatc acaaaatggt tctaactaca agtcttcctc tatctatgct
     6841 atactatgat acaactcaaa cagaaagagg tcatctatgt ttacaaagaa aaatattctt
     6901 actcatctct ttttcctcat catagcagtc ggactaaaac ggtacggtgt cacagcacct
     6961 gacttgacat ggaatatgtt cttggcattg gtagcacttg attttgcaat ccttaccaat
     7021 catacaaagt cccatatcct caaagtgata gcaggtctcc tctggctctt tttctacccc
     7081 aatacttttt acatggtgac agacatcgtc catatgcact ttgccaacac agtactatgg
     7141 caacgagaaa gcatgattct atttatgctt tatgttccaa gtattttctt tggggtcatg
     7201 gctggcgttg aaagtgtacg acttattttc tctgcttttt ctatcaaatc ctactggcta
     7261 aaactgttta tgattagtgg tctttcccta ctatccagct ttgccattca tatcggacga
     7321 tatgcaagac tgaattcatg ggacatcttt accaggccaa ctgtcgttat tgatgaaatg
     7381 cttgcagtta tttcgtggga ttctctcccc tttatcctcg gcttcacctt tatccaaatt
     7441 atggttcttg tcttttcaat aaatgaataa gatttttatc tcatagccat tatcttcaag
     7501 catcttccca cactcaagga ctatcgttcc ttttgggaag atttttcaag gacataaaca
     7561 aaattttttg ataaatttat atcttctccg tttctctcca ttacattttt atccaaactg
     7621 tatgaggcaa aaatcgttaa cctattgtaa gtaaaatttc ttaaatttcc ttatatataa
     7681 aatattcaga
//
LOCUS       DBNFCK010000091         7649 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.091, whole genome shotgun sequence.
ACCESSION   DBNFCK010000091 DBNFCK010000000
VERSION     DBNFCK010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7649
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.091"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(126..6434)
                     /locus_tag="AC1PIW_001481"
     CDS             complement(126..6434)
                     /locus_tag="AC1PIW_001481"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase"
                     /protein_id="HIP6606408.1"
                     /translation="MKKKSVQNMKRTGMLEKKQIFGIRKLNVGVASVGIAAALFLSGA
                     GQLVQAEEVVLPASTSSTTLSEQDGGAVEATTTAIEATDSMVAEATAVTETADATAPE
                     EAPIEEGSIRLHFENVDETAPESQGLWTWGGVAEPSDGNQWPTDTANFSSSQVDDYGH
                     YVDIKKSETPGTIGYLVLKNGEKITESDQKVELLVPEQNEAWIASDYSVSSYEPLKDE
                     NVLRINYTREDNNYEGWGVWTWGDTTEASSGWPAGAVDFKLGKYGAYVDIPLSNGLDS
                     KLGFLLINQNNPDLAGNKTIDLAFADRKRHSQIFLRNDDDKVYTNPYFIEEKVELDTS
                     KATPGTKNVTVGASVKAPFNYNESGLVSVTVTNPENAEIVKMEVDTSAIGGGLVPIST
                     ELNRVTIKATSSTAPGTYSLPVKVYDKDNGYYETKLDVTITERIKADGEKDWDEQVIY
                     FMMTDRFYNGDTGNDKLVEGTASNPRGLYQGGDFKGVTAKLDYLKELGVDSIWLTPIV
                     ENIPQNVGSVKDGEYYAYHGYWASNFEKLNPHLGSLADFHELIDAAAEKGINIIVDVV
                     LNHAGYGTEETFAGMVRTKEEDKQGDDQLGSLSGLPDFKTEEAAVRNQLVAWQASWLE
                     RSTTAKGNSIYAFRVDTVKHVDDTTWQHFKNELVDRDPDFHLIGETWGANYKDTKGDL
                     GIGTMDSLLDFGFKDIAKYLVNGQLKAAGKELEERSKVLTSAASLGQFLGSHDEDGFL
                     YSLGGAEKEGNLDKLKLAASLLITAKGQPVIYYGEEVGQSGQNNWPVYDNRYDFDWSK
                     VETSDITDHYQKLLAFRNANSTLLSRGDTSTLAGNDSQGWLISKRSYQDQAAYLVFST
                     NTENKEMALEVSGKDVVVTDAYTGKSYQAIEKDGKWVVQVELPTIGQGGTMLLQTEAG
                     DIVNASVQGATEEPIEAGYFRVHFKTLPSDNLSSLGLWTWDDVEKPSSDLGAWPTGAT
                     NFSTAKQDDYGYYLDIKMKDETASKISLLINNTSGDNITGDKTIERISTKMNEAWFDE
                     NYQLSLYQPLKEGYIRINYFRTDGNYDQKGLWIWGDVTDLTLGDWPNGIDFENQGKYG
                     AYIDVKLTDLPSSIGFLLLDESKSGDDVKIQQKDYSFKDLKNQTQIFLKDDDATIYTN
                     PYFVNNVRATGVSHVSLTALEAAFTTLEGADKDSILEKLSVTDKNGQTVAVTDLVLDL
                     TSNKVRVLGDFNQENAGYTLKYGNDSFTTTMSWQLKDELYAYDGELGARVRQAGSVVD
                     MTLWSPSADSVAVVLYDKDDQSKVVGKLAMTKGDKGQWDLELNSQSGLGIADYRGYYY
                     HYEITRGDQSVLVLDPYAKSLAEWNSDLADTDPSYRIAKAAIVDPAEVGPSDLNYATI
                     PNFNQREDAIIYEAHVRDFTADPAISDELTAQFGTFAAFAERLSYLKELGVTHIQLLP
                     VMSYYFVNELKNAERMSEYASSNSNYNWGYDPQSYFAFTGMYSTDPTDPMKRIEEFKN
                     LVNEIHKQGMGVILDVVYNHTSKTFLFEDLEPNYYHFMEADGTAKSSFGGGRLGTTHY
                     MSRRVLVDSIKYLVDEFKVDGFRFDMMGDHDAEAIELAFTEAQKLNPNIIMLGEGWRT
                     FTGDANQPVQPADQDWMSSTDTVAVFSDEIRNTLKSGYPNEGQPAFITGGAKSVESVF
                     NNIKAQPGNFLADDPGDVIQYIAAHDNLTLFDIIAQSIKKDPSVTENYTEIHQRQRLG
                     NLLVLTAQGTPFIHSGQEYGRTKQFRHPDYKEPVTEDKVPNKAHLLTNADGTPFDYPY
                     YIHDSYDSSDAVNKFDWTKATDEALYPENTRTQAFTKGLIALRKSTDAFRLGTKEEVE
                     QKVSLISIPGQNGIAKNDVVIAYQTIASNGDRYAVFVNADSKERSFVLSDIYKELLKG
                     QVLVDGERAGVEALSDLVGVELTDSSVVLAPLTATVIRLPYITTALPDTAPTAEEKPS
                     LDFTTKERTEETVLPIAEEVRYDATLAKGQSYVLQEGKAGKRVLVYQDVLVDGKVVAT
                     NLLSETVVDGEARIVVKGSMEAKDVVEKPSLSTPTAQASGQATSGASKASLPITGDRQ
                     SDLALLGLGLAGLGLTVAAQGRTKKSEE"
     gene            complement(6616..7557)
                     /locus_tag="AC1PIW_001482"
     CDS             complement(6616..7557)
                     /locus_tag="AC1PIW_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIP6606409.1"
                     /translation="MTTLADVARLANVSKMTVSRVINHPEQVTQELRTLVTAAMEELN
                     YKPNVAAKALAQQRTLIVQAVILEKMDVVEPYYIDLLAGIADELNHRNYTLQLVTNPS
                     QITDQCDGYIVTGARKDDYPWLKGLRKPLILFGENQEGLPFVDSDNRLATRSATQFAL
                     SKGYERIVFVGIDLPEAFEKGREEGYKDAMQGRETHLYRLENRSRVAEEFVKNLDYLP
                     ENTCFICASDRLALGVTRGLQALGKSIPNDVGVIGFDGFFLDRMSRPALTTMKQPIRQ
                     MGALSVKHLMTILDGKPLARQGHYCQATLIERETTPQ"
BASE COUNT         2016 a         1859 c         1499 g         2275 t
ORIGIN      
        1 aaaatttaaa agaagaggtt ggtttgaaac caacctcttc tagagggaag tgatactctc
       61 aactcttgta tttataagaa attcacaagg ttttgagtaa tgctcctctg gagagccaaa
      121 tattattact cttctgattt tttagttctt ccttgcgcag caactgtcaa accaagtcca
      181 gcaagtccga gacctagaag tgccaaatcg ctttgtcggt cacccgtaat cggcagactc
      241 gccttgcttg caccacttgt cgcttgtcca gacgcttgag ctgttggagt agaaagactt
      301 ggtttttcta ccacatcctt agcctccata ctacccttaa caacaatccg cgcctcacca
      361 tctacaactg tttcagataa cagatttgtt gctactacct tgccgtcaac caagacatct
      421 tgataaacta agactctctt acctgcctta ccttcttgaa gcacataaga ttgacctttt
      481 gcaagtgttg catcatagcg aacttcctct gcaatcggaa ggactgtttc ttctgtacgt
      541 tccttggtag taaagtctag acttggtttt tcttcagcag taggcgctgt atctggaagc
      601 gccgtagtga tataaggcaa acgaatcact gttgcagtca aaggtgcaag aacaactgag
      661 ctatcagtca attcgacacc cactaaatca gacaatgctt ctacacctgc acgttcaccg
      721 tcaaccagca cttgaccctt aagcaattct ttgtaaatat cagaaagaac aaagctgcgc
      781 tccttactgt cggcgttgac aaaaacagca tagcggtccc cattgcttgc aatggtttga
      841 taggcaatga ccacatcatt tttagcaata ccattttgac ctggaattga aatcaagcta
      901 accttctgtt caacttcttc ttttgttccc aaacggaagg catctgtaga tttgcgcaag
      961 gcaatcaatc cttttgtaaa ggcctgagta cgggtattct ctggatagag cgcctcatct
     1021 gtcgctttgg tccagtcaaa cttgttgacc gcatccgatg aatcgtaaga atcatgaatg
     1081 tagtatggat agtcaaatgg tgtaccatca gcatttgtca acaaatgcgc tttgttcggt
     1141 accttgtcct ctgtaactgg ttccttataa tcaggatggc ggaactgttt ggtccgtcca
     1201 tactcctgac cagaatgaat aaacggtgtc ccttgagcag tcaaaactag caaattaccc
     1261 aagcgttgac gttggtgaat ttcagtatag ttttcagtaa ctgacggatc cttcttgatg
     1321 gactgagcga taatatcaaa gagggtcaag ttatcatgcg cagcaatata ctggatcaca
     1381 tcacctggat catctgccaa gaagttgcct ggttgtgcct tgatgttatt gaagacagac
     1441 tcaacactct tagcaccgcc tgtgataaag gctggctgac cttcgtttgg ataaccagat
     1501 ttaagtgtat tgcgaatttc atctgagaat accgcaaccg tatctgttga actcatccag
     1561 tcttggtccg ctggctgaac aggctgatta gcatcacctg taaaggttct ccatccttca
     1621 ccaagcataa tgatgtttgg attgagtttt tgagcctcag taaaggccag ctcaattgct
     1681 tcggcatcat ggtcccccat catatcgaag cggaaaccat ctaccttaaa ctcatccaca
     1741 agatacttga tggaatcaac taagacacga cggctcatgt agtgagtggt ccccagacga
     1801 ccaccaccaa agcttgattt agccgtacca tctgcctcca tgaagtgata atagtttggc
     1861 tctaagtctt caaataagaa ggtcttagaa gtgtggttat agaccacatc caaaatcaca
     1921 cccatgcctt gtttgtggat ttcattgacc agatttttga actcttcgat acgtttcatt
     1981 ggatctgtcg gatctgtaga atacatacct gtaaaggcaa agtagctctg tggatcataa
     2041 ccccagttgt agttactgtt gcttgacgcg tactcactca tccgctctgc atttttcaat
     2101 tcattgacaa aatagtagct cataacaggc aataattgaa tatgagtcac acccaattcc
     2161 ttgaggtagc tgagacgctc tgcaaaggca gcaaaggtac caaactgagc agtcaattca
     2221 tcggaaatag caggatcggc tgtgaagtca cggacatgag cctcatagat aatagcatct
     2281 tcacgctgat tgaagttagg aattgtggca tagttcaaat cacttggacc aacttctgct
     2341 gggtccacaa tcgcagcttt tgcaatccga taagatggat ctgtatctgc caaatcacta
     2401 ttccactccg ctaaagattt agcatatggg tctaaaacaa gaacagattg atccccacga
     2461 gtgatttcat aatggtagta gtaaccacga tagtccgcga taccaagacc tgattggctg
     2521 ttcaattcga ggtcccattg tcccttatcc cccttggtca tggccaattt accgaccacc
     2581 ttagattgat catccttatc ataaagaacg actgccacac tgtctgcact tggagaccag
     2641 agagtcatat caacgacact accagcctga cgcacacgcg caccaagctc tccatcataa
     2701 gcatagagct catccttcaa ctgccagctc atagttgttg tgaagctatc attgccatat
     2761 ttgagggtat agcccgcatt ttcttggttg aaatccccga ggactcgcac cttgttgctg
     2821 gtaaggtcca agacaaggtc cgtcactgca actgtctgac cattcttatc tgttacagac
     2881 aatttctcca agatgctatc cttatccgca ccttccaatg ttgtaaaggc tgcttctaag
     2941 gctgttaggc taacgtgaga aacacctgtc gcacggacat tattgacaaa ataagggttg
     3001 gtgtaaatag tcgcatcatc atccttgagg aagatttgtg tctgattttt caaatcttta
     3061 aagctataat ctttctgctg aatcttcaca tcgtctcctg acttgctttc atccaatagc
     3121 aagaagccaa tggaacttgg taaatctgtc aatttgacat caatataggc accgtacttg
     3181 ccttggtttt caaaatcaat accatttggc cagtcaccca gggtaaggtc agtcacatca
     3241 ccccagatcc agagaccttt ttgatcataa ttgccatcgg tacggaagta attgatacgg
     3301 atgtagcctt ctttaagtgg ttgatagagg ctgagttgat aattctcatc aaaccaagct
     3361 tcattcatct tggtgctaat ccgttcaatg gtcttgtcac ctgtgatgtt atcaccagaa
     3421 gtgttattaa tcaacagact aatcttgctg gctgtttcat ctttcatctt gatatcaaga
     3481 taatagccat agtcatcctg tttagccgtg ctgaaattgg ttgcacctgt tggccaagca
     3541 ccaagatctg acgatggttt ttcgacatca tcccatgtcc ataaacctag gctagataaa
     3601 ttatcagatg gtagagtttt aaagtgaaca cggaagtagc ctgcttcaat tggttcttca
     3661 gttgctcctt gcacacttgc attgacgatg tcacctgctt ctgtctgaag gagcatggtg
     3721 ccaccttgac cgattgttgg cagttcgact tgaacaaccc atttaccgtc tttctcgata
     3781 gcttgataag attttcctgt ataggcatct gtgacaacta catctttacc agatacttca
     3841 agcgccattt ccttgttctc ggtattggtc gagaagacaa gataggcagc ttgatcttgg
     3901 taactgcgtt tgctgataag ccaaccttgg ctatcatttc cagcaagtgt gcttgtatca
     3961 ccacgtgaga gcaatgttga attggcattg cggaaagcca ataatttctg atagtggtct
     4021 gtgatgtcgc tagtctctac cttgctccaa tcgaaatcgt accggttgtc ataaactggc
     4081 cagttgtttt gtccagattg ccccacttct tcaccgtagt aaatgactgg ctgaccttta
     4141 gcagtaatca aaagactagc agccaatttc agtttgtcca gatttccttc tttttctgcg
     4201 ccaccgagac tgtagaggaa accatcttcg tcgtgactgc ccaagaattg acccagagaa
     4261 gctgcactag tcagaacctt gctacgttct tccagctctt ttccagctgc ttttagttga
     4321 ccattgacca gatatttggc gatatccttg aagccaaagt ccaacaaact atccatagtg
     4381 ccgataccca agtcaccctt ggtatcctta taattcgctc cccaagtttc cccaatcaag
     4441 tggaagtcag gatctctatc caccaattca tttttgaaat gttgccatgt ggtatcatca
     4501 acgtgtttga ctgtatcgac acggaaagca tagatagagt tgcccttggc agttgttgaa
     4561 cgttccaacc aagatgcctg ccaagcaacc aactgattgc gaacagcagc ttcttctgtc
     4621 ttgaagtctg gtaatccaga taatgagcct aattggtcat ctccctgctt gtcttcttcc
     4681 ttagtccgta ccatacctgc aaaggtttct tctgtaccat agccagcatg gttaagcacc
     4741 acgtcaacaa taatattgat gcctttttcg gctgcggcat caatcaattc gtggaaatca
     4801 gctaaacttc ccaaatgcgg atttagtttc tcaaaattgg atgcccaata accatgataa
     4861 gcatagtatt ctccatcttt tacgctacca acattctgtg gaatattttc cacaatcgga
     4921 gtcaaccaga tagagtccac acccaattct ttcaagtagt ccagcttggc tgtcactcct
     4981 ttgaagtcac cgccctggta aagaccgcgt gggttggagg ctgttccttc cactagcttg
     5041 tcattgccgg tgtctccatt atagaaacgg tctgtcatca tgaagtagat gacttgctcg
     5101 tcccagtctt tttcaccatc tgccttgata cgctctgtaa tggtcacatc caattttgtt
     5161 tcgtagtaac cgttgtcctt gtcatagacc tttacaggaa gactatatgt ccctggcgct
     5221 gtgctagacg tcgctttaat ggtcacacga ttgagctcag tagagattgg cacaagacct
     5281 ccaccgatgg cacttgtatc gacttccatt ttgactattt cagcattttc agggttggtg
     5341 acggttacag ataccaagcc actttcattg tagttgaatg gtgctttcac acttgcccca
     5401 acagtcacat tttttgtacc tggagtagct ttacttgtat cgagttctac tttctcttcg
     5461 ataaaatatg gattggtata gactttgtcg tcgtcattgc gaaggaaaat ttggctatgc
     5521 cgtttacgat ctgcaaaggc taaatcaatt gttttattgc ctgccaaatc tggattattc
     5581 tgattgatta gcagaaatcc aagcttggaa tctaaaccat tcgatagagg aatgtcaaca
     5641 taggcgccgt atttgccaag tttaaagtca acggcacctg ctggccagcc gctacttgct
     5701 tcagtcgtat ctccccaagt ccatacgccc caaccttcat aattattgtc ttcacgggtg
     5761 tagttgatac ggaggacatt ttcatctttg agtggctcat aactagacac gctataatca
     5821 ctagcaatcc aagcctcatt ttgttccgga acaagtagct ccactttctg gtccgattct
     5881 gttatttttt ccccattctt gagaaccaga taaccaatcg ttcctggtgt ctcagatttt
     5941 ttaatatcaa cgtaatggcc gtagtcatct acctgactgc ttgaaaagtt cgctgtatcc
     6001 gttggccatt gattaccatc ggacggctct gccactcctc cccaagtcca aagtccttga
     6061 ctttctggtg ctgtttcatc tacattttca aagtgcagac gaatgctgcc ttcttcgata
     6121 ggagcttcct ctggagcagt tgcatcggct gtttctgtta ccgctgtcgc ttcagctacc
     6181 atagaatctg tagcttctat cgcagttgtg gtcgcttcta ccgcgccgcc atcctgttcc
     6241 gaaagagtcg ttgaactagt tgacgcaggg aggacaactt cctctgcctg cactagttga
     6301 cctgctccac ttaaaaacag agcagccgca atcccaacag aggctacacc tacatttagt
     6361 ttacgaatgc caaaaatctg tttcttctct agcatccctg ttcgtttcat attctgaacg
     6421 gatttctttt tcatagactc ctccatataa ataagtttga attgtacact accagtatat
     6481 acaattccaa ggagtctttg caaacgtttt cttaaatgtt aacggtaaca agttttgagc
     6541 tactatctaa caaaaataga ataaatagct cccccttctt ttgctcctag accgcccact
     6601 gaccaaaaaa agagactact gaggtgtagt ctccctttca attaaggttg cctggcagta
     6661 atgcccctgt ctagccagag gcttgccatc caatatcgtc attaaatgtt tgacgctaag
     6721 agcccccatt tgacgaatcg gctgcttcat cgtcgtcaag gctggtctgg acatcctatc
     6781 taaaaagaag ccgtcaaaac caatcacccc aacgtcattc ggaatagact ttcctagagc
     6841 ttgtaaacct cttgtaaccc caagagcgag acgatccgat gcgcagataa agcaggtgtt
     6901 ttctggtaaa taatcaagat ttttcacaaa ttcttccgca acacgagaac gattttccaa
     6961 tcgatagaga tgagtttctc ggccctgcat cgcatctttg tagccttctt ctctcccttt
     7021 ttcaaaggct tctggcaagt caatccctac aaaaacaatc ctctcataac cttttgataa
     7081 agcaaactgg gttgcggacc tagtcgccaa acgattgtct gaatctacaa aaggaaggcc
     7141 ttcttgattc tccccaaata aaatcaatgg cttccgcagc cctttcaacc aagggtagtc
     7201 atccttgcga gcgccagtca caatgtaacc atcacattga tcagtgatct gacttggatt
     7261 ggtcaccaac tgcaaggtat aatttcgatg attgagctca tcagcgattc ctgcaagaag
     7321 gtcgatatag tacggctcta ccacatccat cttttcgaga atgacagcct gaacaatcag
     7381 tgtccgttgc tgggccaagg ccttggccgc cacatttggc ttatagttta attcttccat
     7441 cgcagctgtt acgagcgtcc gcagctcttg tgtaacttgt tctggatggt tgatgacacg
     7501 cgaaacagtc atctttgata cattggctaa cctagccaca tcggctaatg tcgtcataga
     7561 actcctccct aattctattt ctgttgctta tcttttagca agcaaacagt caaaattgtg
     7621 caaacgtttt ctttaggtta ttatatact
//
LOCUS       DBNFCK010000092         7604 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.092, whole genome shotgun sequence.
ACCESSION   DBNFCK010000092 DBNFCK010000000
VERSION     DBNFCK010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7604
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.092"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            46..417
                     /locus_tag="AC1PIW_001483"
     CDS             46..417
                     /locus_tag="AC1PIW_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIP6606410.1"
                     /translation="MREKEFRRSLAIFPIGSVMKLTDLTARQIRYYEDQGLIKPDRNE
                     GNRRLYSLNDMDLLLEIKDFLDEGLNIAAIKKEYANREAKAKVQKQQKTLTDEDVRRI
                     LHDEFSRQGRFSSPSSIFRSS"
     gene            446..1792
                     /gene="glnA"
                     /locus_tag="AC1PIW_001484"
     CDS             446..1792
                     /gene="glnA"
                     /locus_tag="AC1PIW_001484"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HIP6606411.1"
                     /translation="MTFTVADIKRDIKEKNVTFLRLMFTDIMGVMKNVEIPATDEQVE
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTIFPWGDENGRVAGLICDIYTTDA
                     KPFAGDPRGNLKKSLRHMEEAGFSSFNLGPEPEFFLFKMDEQGNPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTEMGFEVEASHHEVAVGQHEIDFKYADVLKACDNIQIFKLV
                     VKTIARKHGMYATFMAKPKFGIAGSGMHCNMSLFDKDGNNAFFDPEDPRGMQLSQTAY
                     HFLGGLIKHAYNYTAITNPTVNSYKRLVPGFEAPVYIAWAGKNRSPLVRVPASRGMGT
                     RLELRSVDPTANPYLAMAVLLEVGLEGIQNKIEAPAPVESNIYAMSDEERREAGITDL
                     PSTLHNALKALREDEVVRAALGDHIYTNFLEAKKIEWASYATYVSQWEIDNYLDMY"
     gene            complement(2015..3694)
                     /gene="rnjA"
                     /locus_tag="AC1PIW_001485"
     CDS             complement(2015..3694)
                     /gene="rnjA"
                     /locus_tag="AC1PIW_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J1"
                     /protein_id="HIP6606412.1"
                     /translation="MSSVNLKPHEVGVFAIGGLGEIGKNTYGIEYKDEILIVDAGIKF
                     PEDDLLGIDYVIPDYSYIVENLDRVKGLVITHGHEDHIGGIPFLLKQANVPIYAGPLA
                     LALIRGKLEEHGLLRDATLHEINHNTELTFKHLKVSFFRTTHSIPEPLGIVVDTPQGK
                     IVCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIM
                     KLIEGIHGRIIFASFASNIFRLQQAADAAVKTGRKIAVFGRSMEKAIVNGIELGYIKV
                     PKDTFIEPNEIKEYPASEIMILCTGSQGEPMAALSRIAHGTHRQVQLQPGDTVIFSSS
                     PIPGNTTGVNKLINILIEAGVDVIHGKINNIHTSGHGGQQEQKLMLRLIKPKYFMPVH
                     GEYRMQKIHASLAVDTGVPKDNIFIMENGDVLALTKDSARLAGQFNAQDIYVDGNRIG
                     EIGAAVLKDRRDLSEDGVVLAVATVDFNSKMILAGPDILSRGFIYMRESGELIRESQR
                     VLFNAIRIAMRNKDANIQTVNGAIVNALRPFLYEKTEREPIIIPMILTPDR"
     gene            complement(3698..3928)
                     /locus_tag="AC1PIW_001486"
     CDS             complement(3698..3928)
                     /locus_tag="AC1PIW_001486"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase subunit epsilon"
                     /protein_id="HIP6606413.1"
                     /translation="MIYKVFYQESKISPRREQTRALYLDIDAASELEGRIIARELVET
                     NRPEYNIEFIDLLSDNHLEYEKEHADFKITEF"
     gene            4535..5218
                     /gene="tsaB"
                     /locus_tag="AC1PIW_001487"
     CDS             4535..5218
                     /gene="tsaB"
                     /locus_tag="AC1PIW_001487"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIP6606414.1"
                     /translation="MKILALDSSNQALSVALVEDGRLQAETLLAVKKNHSISLMPVVD
                     FLVAQVGWTPKDLDRIVVAQGPGSYTGLRVAVATAKTLAYTLKIDLVGLSSLQSLVPP
                     SLTGLVVPLIDARRNCVYAGVYENGRAVEPDRYWSFEDLLSSLSGKENITFVGEVENF
                     MEQIEQALPTAQYQASLPSAYQLAVIGQDLPAVDVTSFEPNYLKHVEAEENWLKDNQV
                     GSESYIKRV"
     gene            5215..5655
                     /gene="rimI"
                     /locus_tag="AC1PIW_001488"
     CDS             5215..5655
                     /gene="rimI"
                     /locus_tag="AC1PIW_001488"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HIP6606415.1"
                     /translation="MIEIKHYDGQENLAEAVLAVMQSVYDQSPWTLEQIVSSMTSQYE
                     DYYLAYEGQELVGFLAVQTVLDEMEILQIAVKTDFQRLGIASQLMAAVMDWDGDIFLE
                     VRESNSAAQALYTRQHFTKIGKRKDYYRHPVEDAVIMKRERDER"
     gene            5645..6652
                     /gene="tsaD"
                     /locus_tag="AC1PIW_001489"
     CDS             5645..6652
                     /gene="tsaD"
                     /locus_tag="AC1PIW_001489"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIP6606416.1"
                     /translation="MKDRLILAIETSCDETSVAVLRNDAELLSNVIASQIASHQRFGG
                     VVPEVASRHHVEVITACIEEALLEAEVTAEDLTAVAVTYGPGLVGALLVGISAAKAFA
                     WANGLPLIPVNHMAGHLMAARAVKELEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLPYPAGRVIDELAHEGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLYHNAQQKGETLSNADLSASFQACVMDILMAKTKKALEQYPVKTLVVAGGVAANQ
                     GLRERLAAEITDVEVIIPPLRLCGDNAGMIALAAVSEYNKENLAGWDLNAKPSLAFEN
                     L"
     gene            complement(6689..7525)
                     /locus_tag="AC1PIW_001490"
     CDS             complement(6689..7525)
                     /locus_tag="AC1PIW_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIP6606417.1"
                     /translation="MKIQPEFYVFNNRSYVDFYPIQFGMEACKPLHSFGPTMKQHYLF
                     HYIISGSGTFYDTSDQREYHLTAGQGFLISPDAICSYEADEKDPWTYIWLEFDGLKTE
                     HFLRQAGLSKEQPIFSQREMPTSSPVYHEMKQILALHHQKSALVLGHLYLFISCLIDC
                     SLTKTSSKHDENKEFYIREAINFVERNYEKAISVDDLAQVCNLNRHYFSRLFKEQMNI
                     SPQQFIIQYRLSEACELLKNTTKTLQEIAEEIGYSNQFNFSTAFKRHYQISPNRWRKI
                     HK"
BASE COUNT         2004 a         1481 c         1970 g         2149 t
ORIGIN      
        1 tcaaaaaaaa attctcctat aatggtaggc agaaaggagt tcattatgag agaaaaagag
       61 tttcgtcgtt ccttagctat ctttccgatc ggtagtgtga tgaagttgac agatttaact
      121 gctcgtcaaa ttcgttacta tgaagatcaa ggcttgatta agcccgatcg caacgaaggg
      181 aatcgtcgct tgtattctct aaacgatatg gacttacttt tagaaatcaa ggattttttg
      241 gatgaaggtt tgaatattgc tgcgattaag aaggaatatg caaatcggga agcgaaagct
      301 aaggtccaaa aacagcagaa aaccttgact gatgaagatg tccgtcgtat cttacatgat
      361 gaattcagtc gtcagggacg attttcaagt cctagttcca ttttccgttc gtcgtaatta
      421 gcttttaaaa tagaggagaa aaataatgac attcacagtg gcagatatca agcgcgatat
      481 caaagagaaa aacgttacct tcctacgtct tatgtttaca gacatcatgg gagtgatgaa
      541 aaacgtagaa attccagcaa ctgatgagca ggtcgaaaag gttttgtcaa ataaggtcat
      601 gtttgatggt tcatctattg aaggatttgt tcgtatcaat gaatcagata tgtatcttta
      661 cccagatttg gatacatgga ctatcttccc ttggggtgat gaaaatggcc gtgtagctgg
      721 cttgatttgt gatatttata caacagatgc taagcctttt gctggtgacc cacgtggaaa
      781 tttgaagaaa tcgcttcgtc acatggaaga ggcaggtttc tcttcattca accttggtcc
      841 agaaccagaa ttcttcctct ttaagatgga cgaacaagga aatccaacgc ttgaagtaaa
      901 tgataaaggt ggttactttg acctcgcacc aacggacttg gctgataata cacgtcgcga
      961 aatcgtcaat gtcttgacag agatgggctt tgaagttgaa gcgagtcatc acgaagtggc
     1021 agtaggtcag catgaaattg acttcaaata tgcggatgtc ttaaaagctt gtgacaatat
     1081 tcaaatcttc aagttagttg ttaaaacaat tgctcgtaag catggcatgt atgcgacctt
     1141 tatggccaag cctaagtttg gtattgctgg ctcaggtatg cactgtaaca tgtccctctt
     1201 tgacaaggat ggaaacaatg ccttctttga tccagaagat cctcgcggta tgcaactgtc
     1261 tcaaactgct taccatttcc ttggtggttt gattaagcat gcttataact atactgctat
     1321 tacaaacccg acagtgaact cttacaagcg tttggttcca ggctttgaag ctcctgtgta
     1381 tattgcttgg gctggtaaaa accgctcacc attggttcgc gtacctgcat cacgtggaat
     1441 gggaactcgt ttggaattgc gctcggttga tccaactgcc aacccatact tagctatggc
     1501 agttctattg gaagttggct tggaaggtat tcaaaataaa attgaagcac cagcaccagt
     1561 agaatctaat atctatgcaa tgtctgatga ggagcgtcgt gaagcaggta tcactgatct
     1621 tccttcaact cttcacaatg cattgaaagc tcttcgtgaa gacgaggtag ttcgtgcagc
     1681 tctaggtgat catatctata cgaatttctt ggaagctaag aaaatcgaat gggcaagcta
     1741 cgcaacctac gtttctcaat gggaaatcga caactatttg gatatgtatt aaacaaagga
     1801 ccccgacggg tcctttgttc acgagccgag aaattggaaa tcgggtaatg gtccagtgga
     1861 gtgaagtggt ctggaaagag actgtttcag tctgagctaa taaatagcag ggcgagggga
     1921 tagacatgag cttgaaatct gtcttttttc aagctgggtt atcttgttaa tataaaataa
     1981 atcccagagg tttcacctct gggattttag tgtgttatcg gtcaggcgtc agaatcattg
     2041 ggataatgat tggttcacgc tctgtttttt cgtataggaa tggtcgtagg gcgttaacaa
     2101 tggcgccgtt gactgtttgg atatttgcgt ctttgttgcg catggcgatg cggatagcgt
     2161 tgaaaaggac gcgttggctt tcgcgaatga gctcgccaga ttctctcata tagataaagc
     2221 cacggctgag gatgtctggt cctgctaata tcatcttaga attaaaatcg acagtggcta
     2281 ctgcaaggac aacaccatct tctgaaagat cgcggcggtc ttttagaaca gctgcgccga
     2341 tttcaccgat gcggtttcca tcaacataga tgtcttgggc gttgaattga cctgcgagac
     2401 gtgcagaatc ttttgtcaga gcaaggacat ctccgttttc catgatgaag atattatctt
     2461 tgggaacgcc tgtatcaaca gctaggctgg catggatttt ttgcatgcgg tattcaccat
     2521 ggacaggcat gaagtatttg ggtttgatca agcggagcat gagtttttgc tcttgttgac
     2581 caccgtgtcc agaggtatgg atgttgttga tcttgccgtg aataacatcg acaccggctt
     2641 caatcaagat attgatgagc ttgttgacac ctgtggtatt tcccggaata ggactggacg
     2701 agaagataac cgtgtcgcct ggctggagtt gcacctgacg gtgggtaccg tgggcgatgc
     2761 gagagagtgc ggccataggc tcaccctggc tacctgtaca gagaatcatg atctcgctgg
     2821 cagggtattc tttgatctcg tttggctcga tgaaggtatc cttaggcacc ttgatgtagc
     2881 cgagctcgat accgttaaca atggcttttt ccatggaacg tccaaagaca gcgatcttgc
     2941 gtccggtctt aaccgctgca tcagccgcct gttggagacg gaagatgttg gaggcaaagg
     3001 aagcaaagat gatgcgtccg tgaatgcctt caatcagctt cataatggac tgaccgacca
     3061 ctttttccga gttggtgaag gttgggactt cagcatttgt cgagtcggaa aggaggcaga
     3121 gaacgccttc ttcacccagg gcggccatgc ggtgaaggtc tgctggctca ccaacaggag
     3181 tgaagtcgaa cttgaagtca ccggtacaga cgattttccc ttgaggtgta tcaacgacga
     3241 taccgagagg ttctggaatg gagtgggtgg tacggaagaa gctgactttg aggtgcttga
     3301 aggtcaactc tgtattatga ttgatttcat gcaaagtcgc atcacggagc agaccgtgtt
     3361 cttccaattt gcctcgaata agggcgaggg ctaaaggtcc agcatagatt gggacattgg
     3421 cctgcttgag taagaaggga atgcctccga tgtggtcttc gtgcccgtgg gtaataacca
     3481 gacctttaac gcggtctagg ttttcaacga tgtaggagta gtctgggata acgtagtcga
     3541 tacccaatag gtcatcttct gggaacttga taccggcatc gacgatgaga atttcatcct
     3601 tatactcgat accgtaggtg tttttcccga tttctcccaa accaccgatg gcgaaaacgc
     3661 caacttcatg aggttttaga ttgactgatg acatggatta aaactccgta attttgaaat
     3721 ctgcgtgctc tttctcgtat tcaagatgat tgtctgataa gaggtcgata aattcgatat
     3781 tgtattcagg gcggttggtt tccaccaatt cacgcgcgat aatacgacct tccaattctg
     3841 atgcagcatc gatgtctaga taaagagcac gtgtctgctc gcgacgtggg ctaatttttg
     3901 attcttgata gaatacttta taaatcatgt gtagtatagt tcctttcttt ttgactggca
     3961 actatgaaaa aagcctcaaa cagttgggtc tgtctggtaa tatctattca attttctcga
     4021 gttgtcaatt tattcagtct aaatgtactc ttcattatac cataaaatat gctgatggta
     4081 aaagggtttg gtaggaaaat ggaaaagtgt catgattttc agaaattcgg tatttcagaa
     4141 gggtataaat caaagaaatg agttaggatt ccgagtgtgt tgggattact aagactaagt
     4201 tcaattgtgc tgagaaatgc ctatcaactg ccagatagtg atgtgactta ctagaatgac
     4261 aaatcggttc gatccattaa cctactagaa atcacacccc agtcttttcc attatttgaa
     4321 aaaatatcca agatagctta caatagagag tagcatttaa aagtctttca gtttgctttt
     4381 agtactaggc aacgagctgc gggctgtact ggagtacggc acagggagtt caaacaatac
     4441 agtgtattgt ttgaagtggg aaatacggaa acgaagtttc ctcaagtgtt gaagtaataa
     4501 aagcaaaccg aatgacgtaa agaaagagaa acttatgaaa atactagctt tggacagctc
     4561 caatcaggcc ttatcggttg ccttggtgga ggacggtcgt ttgcaggcgg aaaccctgtt
     4621 ggctgtcaag aaaaatcata gtatcagcct catgcctgtg gttgattttt tggtagcgca
     4681 ggtgggctgg acacccaaag acctggatcg gattgtggtc gctcagggac ctggctccta
     4741 cacgggcctg cgggtggcgg tggcgaccgc caagaccttg gcctacaccc tcaagatcga
     4801 cttggtgggg ctatctagtc ttcagtctct ggttcctcct agcctgacgg gtctggtggt
     4861 gcctctcatt gatgcccgcc gtaactgcgt ctatgcaggt gtttatgaaa atggcagagc
     4921 agtcgagcca gaccgctact ggtcttttga agacttgctt tctagcctgt ctggtaagga
     4981 aaatattacc tttgtcggag aagtagaaaa ctttatggag caaatcgaac aagctctacc
     5041 gactgctcag tatcaagcta gtctgccgtc tgcctatcag ctagcagtaa tcggtcaaga
     5101 cttgccagca gtagatgtga cgagctttga gcccaactat ctcaagcatg tagaggcaga
     5161 agaaaactgg ctcaaggaca accaagttgg ctcagaaagt tatatcaagc gtgtatgata
     5221 gagattaaac actacgacgg tcaggaaaat ctggcggagg ctgtgttggc agttatgcag
     5281 tccgtctatg atcagtcacc ttggaccttg gaacagattg tttcaagcat gaccagtcag
     5341 tatgaggatt attatttggc ctatgagggt caggaattgg tcggattttt ggctgtgcag
     5401 acggtgctgg atgagatgga aatcttgcag attgctgtca agactgattt tcagaggctg
     5461 ggaatcgcca gtcaactgat ggctgctgtg atggactggg atggggatat tttcctcgaa
     5521 gtgagagagt ccaatagcgc agctcaggcc ctctatacac gccagcattt taccaaaata
     5581 ggaaaacgaa aagactacta ccgccatcct gtcgaggacg cggtgataat gaagagagaa
     5641 cgtgatgaaa gatagattga ttttggcgat cgagacctcc tgtgacgaga cctcggtggc
     5701 tgttttgcga aatgacgcgg agcttttatc caacgtcatt gccagccaga ttgccagcca
     5761 ccagcggttt ggtggggtgg tgcccgaggt ggccagccgt caccatgtgg aagtgataac
     5821 ggcctgtatc gaggaggcct tgttggaggc ggaagtgacg gcagaggacc tgacggctgt
     5881 ggctgtgacc tatgggcctg gcttagttgg tgccctgttg gtcgggattt cagctgctaa
     5941 ggcctttgcc tgggctaacg gtctgccgct cattcctgtc aaccacatgg cagggcattt
     6001 gatggcggcg cgggcggtca aggagttgga gtttccgctc ttggctcttt tggtcagcgg
     6061 tgggcacacg gagttggtct atgtgtcaga ggcaggtgac tacaagattg tcggtgaaac
     6121 gcgagatgat gcggtcggcg aagcctatga taaggtgggt cgggtcatgg gcctgcccta
     6181 tccagctggt cgggtgattg acgaattggc acatgagggg caagacattt atgacttccc
     6241 tcgggctatg attaaagagg ataatttgga gttttctttc tctggtttga aatccgcctt
     6301 tatcaacctc taccacaatg cccagcagaa aggggagacc ttgtccaatg cggacttgtc
     6361 agcctctttc caagcctgtg ttatggacat tctcatggct aagaccaaga aggccttgga
     6421 gcaataccct gtcaaaacct tggtggtagc gggcggtgtg gcagctaatc agggcttgcg
     6481 agagcggttg gctgctgaaa ttaccgatgt ggaggttatc attcccccgc ttcgcctctg
     6541 cggtgacaat gcaggtatga ttgccttagc agcggtcagt gagtacaata aggaaaacct
     6601 tgctggctgg gacctcaatg ccaagcctag tctggctttt gagaatttat agaataaaaa
     6661 cctcttacgt caaggatacg taagaggttt atttgtggat ttttcgccag cgatttggag
     6721 aaatttggta atgtctttta aaagcagttg aaaaattgaa ttggttggag tagccgattt
     6781 cttcagcaat ttcctgtagt gtcttagtag tgttttttaa gagttcgcag gcttcgctga
     6841 gtcggtattg gatgataaat tgttgaggtg agatgttcat ttgctccttg aatagccgac
     6901 tgaagtagtg gcgattgagg ttgcacactt gtgccaaatc atcgacggat atagctttct
     6961 cataatttcg ttctacgaag ttgatagctt ctcgtatata aaattccttg ttttcatcgt
     7021 gtttgctact tgttttggtt agcgaacagt caatgaggca ggagataaag aggtaaaggt
     7081 gtccgagaac tagagcgctt ttttgatgat ggagcgcgag aatttgtttc atttcatggt
     7141 agacagggct agaggtgggc atttctcgtt gtgaaaagat aggttgttct ttcgagagcc
     7201 ctgcctgtct gagaaaatgc tcagttttca gaccgtcaaa ttcaagccag atataggtcc
     7261 aggggtcctt ttcgtctgct tcgtagctac agatggcgtc tggggagata aggaagccct
     7321 gtcctgcggt taggtggtat tcacgttggt cggaggtatc gtaaaaagta cctgaaccag
     7381 agatgatgta gtgaaacagg tagtgttgtt tcatagttgg gccgaatgag tgaagtggct
     7441 tacaggcttc catgccaaat tggatggggt aaaaatctac ataggatctg ttattaaaga
     7501 cgtaaaattc aggttgtatt ttcatcgtat tctccatacc aaaagtgtaa aggcttacaa
     7561 ttagtatagc atatttatat gtaaatgtga cattttattt gaaa
//
LOCUS       DBNFCK010000093         7483 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.093, whole genome shotgun sequence.
ACCESSION   DBNFCK010000093 DBNFCK010000000
VERSION     DBNFCK010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7483
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.093"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(134..1411)
                     /locus_tag="AC1PIW_001491"
     CDS             complement(134..1411)
                     /locus_tag="AC1PIW_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIP6606418.1"
                     /translation="MNKQTYKEILTIAFPAMGENLLQMLMGVVDSYLVASIGIVALSG
                     VSLANNILAVYQAVFIALAVAVSSRLAQALGEGGSEGIGGTASESVKFTVFIGLLLGI
                     FSVLAGPIVLTSLGAESEVVQAGGLYLILVGGGAPFLGLMTSLSAILRTLGEPRFPMY
                     ISLLSNVLNALFSAFAVFVLHAGVAGVAIGTVLSRLIGCSLLWSRLPISLKPWTWSFD
                     KELLRLALPATGERLMMRVGDVVVVALITSLGTATVAGNAIGETLTQFNYMPALGIAT
                     ATIILTAKHRQDKAMVRHIFRTSFGLSLLFMFLVAGVTYFAGSFLIDLYTKDIQAAQA
                     SQTVLFYAMLGVPFTAGTLIMTALWQGLGNARLPFYATTIGMWLVRIGIAYLLVTFFK
                     IGLSAIWIATILDNAFRSGVLFFQYRYSKQESI"
     gene            complement(1417..2844)
                     /locus_tag="AC1PIW_001492"
     CDS             complement(1417..2844)
                     /locus_tag="AC1PIW_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6606419.1"
                     /translation="MLSRFSDSRPAVTVKNVALTYSGADKPALQIEDLTIPEGQCVVL
                     CGQSGSGKSSFLKVLNGLTPEYYPAQLSGQIFLGDLDLQKSSLEEISRHIASVFQHPS
                     TQFFHSQVLQELVFPCENQGLSREEIENRLAWVMELFGLASLSQRTISSLSGGQQQRL
                     ALAVATMQGTDVLVLDEPTANLDQEGIVIVEDILKTVKSQGKTIIIAEHRLSYLSQLA
                     DRYLYFQDGQVVTDYLADEFLSRTEECRQDMGLRCYDSEPYGRAIAELADQFVNDKEG
                     LLVENLSVSQKENTLYEIEKLCLAPGQVVGLVGANGSGKTSLARYLVGLAEDKKSRIS
                     WQGQTLSSRQRLEKTAFVMQDVRLQLFAESVERELTLGRKNRAVDEHLVARFGLSDLL
                     ERHPVSLSGGEQQRVMIVASLLADKEIFIFDEPTSGLDLRRMQQVASALVDLKMKNKL
                     VLLISHDEELLNLVCDKIVDIKQLK"
     gene            complement(2808..3467)
                     /locus_tag="AC1PIW_001493"
     CDS             complement(2808..3467)
                     /locus_tag="AC1PIW_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HIP6606420.1"
                     /translation="MKLDARTKIILVIFTSFTYGMRLTILENAVLVLGLSLLFWFSGK
                     RKMAIMSPIAYSIFCLLSYITFLPAWLTHLLLVLTYTWPPLLAGHLLLMTTSGYELIH
                     GLRKWHLPEVFLLTLGVMFRFLPAIKQDARTICASLKVRGIFLRKRDVVCKPLQYMEF
                     FLVPLMMSLLRTAQELTVASLTKGLAVSTKSPAYIWSSWTVLDWSLCLCCLGFLILVR
                     L"
     gene            complement(3464..4057)
                     /locus_tag="AC1PIW_001494"
     CDS             complement(3464..4057)
                     /locus_tag="AC1PIW_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HIP6606421.1"
                     /translation="MKKLQVKDLMVTGAFAALYFVCVGLGTLLSLVFDRSGNMMYAPA
                     GAALLAGPVYMLLVAKVGKFGSISLVGAVMACFFFLSGYMTAAFLPSLTFGFLAEMVA
                     KSGHYKQKWTNLFSYIIFSFGNLGPIILMWFMRDAYEANLLARGKSAEYIARVMLDFT
                     PENVLWLSTTIILTALISGLFGQYMLQRYFKQSGYLS"
     gene            complement(4058..5719)
                     /locus_tag="AC1PIW_001495"
     CDS             complement(4058..5719)
                     /locus_tag="AC1PIW_001495"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding/permease
                     protein"
                     /protein_id="HIP6606422.1"
                     /translation="MDKKEMPTRVLIPRLLQSMKHLLPRIAVAVCFAVLGQVVTIAIP
                     VMLVYLAFNALTGNPAPLWTLGILILLALLRGAFRYGEHYFGHYVAFHTLAAYRRLIF
                     AKLRALAPGKLDRQDSGSLLKMIGEDIEALEVFFAHTIAPICTGILVALGLTVYFGLS
                     SWGLAFLALVTYALLAIAIPNQFAQQLQPLLKEQNASRKGYVSYFIESLKSMKDLMQF
                     QQTDARFANLTQKSQEVNGQERRVAQTNFMQYAVSFLVVGLSIMGFAWLTFDLVGSQS
                     LDLATGVALLVSFISSFAPFLELSRLPLGFKRAMNAGRNIYALLDEKEAERTGELVEV
                     SVTDIAIEQLDFDYDVRETGLYRNLSVQFEQGGIIGLVGESGAGKSTLMKLIMRWYDW
                     QQGQIRLSGLDSRQVDKAHLQGAFAYVPQVPQIFRQTIRENLVLGRTDISDETIMDLA
                     EKCHMKERILAAPQGLDTLVEASDFSAGEGQRLELMRALLKNADCYIFDEPTSNLDSL
                     NEARFIQLVKEHCQGMVFLISHRSSTMACADTIFRLEDGQLVKEK"
     gene            complement(5712..7472)
                     /locus_tag="AC1PIW_001496"
     CDS             complement(5712..7472)
                     /locus_tag="AC1PIW_001496"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HIP6606423.1"
                     /translation="MAQEQEKISREKKKALLKRLKERIKPKMKLVYLAAFLSWVQFLM
                     RIISFYLIAKGFVSYYEGGQVDLVRFVLILLGLNAFGYGVALIAKRLQGLGSQFARDS
                     LKQSFFEVLLAKDGQFASKATAADVFNIASQGIDSLDTYYSYYMASSLRTQFNCATVL
                     LLVFLIFPLGAVIFILALPLIPISIIAMQKRSKRIMNRYWGSYMDVGNLFLDDLKGLN
                     TLYSYQADATYEKTFNEQAEDFRDATMELLSFQLQAVGYMDAVMYLGIGLSGFVAVNS
                     LAAGNLSLFSMLFFVLIATEFFAPIREQGYGMHLVMMNTKMADRIFGFLDSMTAEQEI
                     DSVHVPAFDSLKLEDLAFAYGEKPVLEDISMTMPAGKVYALAGESGQGKTTLAQLLLG
                     RLRADKGAIYLGEQEISGISKLSLNEQVLYVSGQSTLLNQSIYHNLRMACDWSKEDIL
                     AWADQHGVLQFVKNLPDGLDTIVGDDGAFLSPGQRQQVICARAVLAKRSLYIFDEVTS
                     SVDQDNEGLIYDLIDLVAKDAIVIIITHKMKQVEQADDILFLSAEGAVTGNYATLYQT
                     SLAYGQLVDQQRELEEAVYG"
BASE COUNT         2308 a         1920 c         1481 g         1774 t
ORIGIN      
        1 cacgaaaaca gccgaccgcg gccgactgtt ctctaggaga ttatatgaaa aagaaagttg
       61 ttcgctattt ctagcttgaa tatagtatat agctacaaac ttaaaagaaa ctgaaaattt
      121 tcttagcctt atcttaaatt gattcttgtt ttgaatacct atactgaaaa aatagcaccc
      181 ctgatcggaa ggcattatct aaaatcgttg caatccaaat ggctgacaaa ccgattttaa
      241 aaaatgttac aagtaaataa gctatgccaa tccgcactag ccacataccg atagttgtgg
      301 cataaaaggg taatctagca tttcccaaac cttgccaaag agctgtcata ataagagtgc
      361 cagctgtaaa gggaactccc aacatggcat aaaataatac tgtttgacta gcttgtgctg
      421 cttgaatatc cttagtataa aggtcgatta ggaaagaccc cgcaaaataa gtcacaccag
      481 ccaccaaaaa cataaacagc aaagaaaggc caaagcttgt tcgaaaaata tgtcgaacca
      541 tagccttatc ctgtctgtgt ttcgctgtaa ggatgattgt tgcggtagca atccctaaag
      601 ccggcatata gttaaactgc gtcagggttt ctccaatcgc atttccagct accgtcgctg
      661 ttcctaagct ggtaatcaac gccacgacta ccacatctcc cacccgcatc atcaaccgtt
      721 caccagttgc tggcagggca agtctaagca gttctttatc aaaggaccag gtccatggtt
      781 ttaaagaaat tggtaagcga gaccaaagca ggctacaacc aatcaaccgc gataataccg
      841 tgccaatcgc aactccagct acacctgcat gaagcacaaa gactgcaaat gctgaaaata
      901 gtgcatttag aacgtttgag agcagactga tatacatagg aaaacgtggt tcccccaagg
      961 ttcgtaagat tgcactcaaa cttgtcatga gtcccaaaaa aggtgctccc ccaccaacca
     1021 atatcaaata aagaccccca gcctgtacca cttctgactc tgcccccaag ctagtaagta
     1081 ctattggacc tgccagaact gaaaaaattc ctaaaaggag accgataaaa actgtaaact
     1141 tgacactttc actagcagtt cctccaatgc cttctgaacc cccttcaccc aaagcctgag
     1201 ccaaacgaga agacaccgct actgctaggg caataaaaac cgcctgatag acggctaaaa
     1261 tattattagc aagggaaact ccagacaaag ctacaatgcc aatactggct actagataag
     1321 agtctactac ccccattaac atctggagaa ggttttcccc catggctgga aaggcaatgg
     1381 ttaaaatttc tttataggtt tgtttgttca taatcactat ttcaactgtt taatatctac
     1441 tattttatca caaaccagat tcaatagctc ttcatcgtga gaaattaaca aaaccaactt
     1501 attcttcatt tttaagtcta ctaaagcgga agcaacttgt tgcattcgtc tcaaatccaa
     1561 gccgctcgtc ggttcatcaa aaatgaaaat ttccttatct gccagtaagc tagctactat
     1621 catcacgcgc tgttgttcac cacccgataa actgaccgga tgtctctcca acaaatccga
     1681 tagaccaaaa cgagccacca aatgttcatc gacagctcta tttttccttc caagagtaag
     1741 ttctcgctca acactttccg caaatagttg gagtcgaaca tcctgcataa caaaggctgt
     1801 tttttccaat ctctggcgac tagataaggt ttgaccttgc caagaaatcc tactcttctt
     1861 atcttccgct aaaccgacca gataacgagc aaggcttgtt tttccagaac cattcgctcc
     1921 aaccaaaccc accacctgtc ctggtgccaa gcagagtttt tcaatctcgt agagagtatt
     1981 ctctttctga cttaccgata aattttcaac aagtaacccc tctttatcat taacaaactg
     2041 gtctgccagc tccgctattg cccgcccata aggctccgag tcataacaac gcagtcccat
     2101 atcctgacgg cattcttctg tccgacttaa aaactcatct gctagataat ccgtcaccac
     2161 ttgcccatct tgaaaatata aatagcgatc tgccaattga ctgagataag acagtctatg
     2221 ctcggcaata ataatggtct tgccttggct ttttacagtt ttcaagatgt cctctacaat
     2281 cacaatccct tcctgatcta ggttggcagt cggctcatct aaaaccaaga catctgtccc
     2341 ttgcatagtt gcaacggcca aggctagacg ttgttgctga ccacctgata gactggaaat
     2401 cgttcgctgg ctaagagaag ccaaaccaaa taattccata acccaagcca ggcgattctc
     2461 gatttcttct cttgacaaac cctgattttc acaagggaaa accaattcct gcaagacctg
     2521 actatgaaaa aattgagtag acggatgctg aaagactgaa gcaatgtgcc gagatatttc
     2581 ttccagacta gatttttgta aatccaagtc ccctaagaaa atttgtcccg aaagctgagc
     2641 aggataatat tctggcgtca aaccatttaa taccttgaga aaactagact tcccagaacc
     2701 actctgccca cataaaacaa cacactgtcc ttctggaatg gtcagatcct ctatctgcaa
     2761 ggctggttta tctgctcctg aataagttaa tgctacattt tttaccgtca cagccggacg
     2821 agaatcagaa aaccgagaca acataaacaa agactccaat ctagcacggt ccaagacgac
     2881 caaatatatg caggtgattt cgtagacaca gccaaacctt ttgtcagact ggctactgtc
     2941 aattcctgag ctgttcgcaa gagagacatc atcaagggaa ccaagaaaaa ctccatgtac
     3001 tggagtggct tacaaaccac atctctcttc cgtaaaaaaa ttcctctaac cttcaaagag
     3061 gcacaaatcg tacgagcatc ctgtttaatg gcaggtagga aacgaaacat tactcccaac
     3121 gtcaaaagaa atacctctgg caggtgccat ttccgcaagc catgaatgag ttcgtaacca
     3181 cttgtcgtca tcagtaatag atgtcctgcc aaaagcggag gccatgtata ggtcaataca
     3241 agtaacaaat gcgtcagcca tgctggtaaa aatgtaatat aggacaacag acaaaaaata
     3301 gaataggcta tcggactcat tatagccatt ttccgctttc cagaaaacca gaaaagcagg
     3361 ctaaggccta agaccagcac tgcattttct aatattgtaa gcctcatccc ataggtaaaa
     3421 ctagtgaaga tgacgagaat aatttttgta cgagcatcta atttcatgaa agataacctg
     3481 attgtttaaa atagcgctga agcatatatt gaccaaacaa accactgata agagctgtta
     3541 agataatggt cgttgacaac catagcacat tctctggagt gaaatcaagc atgacacgag
     3601 cgatgtattc tgctgatttt ccacgtgcca aaagattggc ctcataggca tctcgcatga
     3661 accacataag aataataggt cctaagtttc caaaagagaa aatgatatag ctaaaaagat
     3721 tggtccattt ttgtttatag tgacctgact tcgctaccat ttctgccaaa aaaccaaagg
     3781 tcaaacttgg gagaaaggcc gctgtcatat agcctgatag gaaaaagaaa caggccatta
     3841 ctgctccaac cagactaatt gaaccaaatt taccaacctt ggctaccaat aacatataaa
     3901 caggtcctgc caaaagggct gctcccgcag gtgcatacat catattaccc gaacgatcaa
     3961 aaaccaagct caacaaggtt cctagaccaa cacaaacaaa gtagagggca gcaaaagccc
     4021 ctgttaccat cagatctttt acttgtaatt tcttcattta tttttccttt actaattgtc
     4081 cgtcttccaa gcggaaaatg gtatccgcac aagccatggt cgagcttcta tgagaaatca
     4141 agaagaccat gccctggcag tgttccttaa ccagttggat aaagcgggct tcattgagcg
     4201 aatctaggtt actagttggc tcatcaaaga tatagcaatc cgcatttttg agcagggccc
     4261 gcatgagttc caagcgttgc ccctctcctg ctgaaaaatc actggcctcc acaagcgtat
     4321 ccaagccctg cggtgctgcc aaaatccgtt ctttcatatg gcatttttct gccaagtcca
     4381 tgatggtttc atccgatata tccgtacgac ccaagacaag attttcccga atggtctggc
     4441 ggaagatttg tggcacctgt ggtacatagg caaaagcccc ctgcaaatga gccttatcta
     4501 cctgccgact atccaagcca gacaggcgga tttggccttg ctgccaatca taccaccgca
     4561 taattagttt catcaaggtg gattttcctg caccagactc accaaccaga ccaatgatgc
     4621 ctccctgttc aaactggaca gacaggttac gataaaggcc tgtttctcgg acatcatagt
     4681 cgaagtccag ttgctcaata gcaatgtctg tcacgctgac ctcaacaagc tcacctgttc
     4741 gctctgcttc tttttcatcc aaaagagcat agatgttgcg accagcattc atagcccgct
     4801 tgaaaccaag aggcagacgg ctgagttcca agaagggagc aaaggagcta atgaaagaaa
     4861 ccagcagggc tactcctgtt gctaagtcca aactctgact acccaccaag tcgaaggtca
     4921 gccaggcaaa gcccatgata gacaagccga ccaccaagaa ggagacggca tactgcatga
     4981 agttggtctg ggcgaccctt ctctcctgac cattgacctc ttggcttttc tgagtcagat
     5041 tggcgaagcg agcatctgtc tgctggaact gcatgagatc cttcatgctc ttaagacttt
     5101 caataaagta ggacacatag cccttacggc tagcattttg ctccttgagc aagggttgta
     5161 actgctgagc aaactgattt ggaatagcaa tcgctaaaag agcataggtc accagagcca
     5221 gaaaagccaa accccaacta gatagaccaa aatagaccgt caaaccaaga gccaccaaaa
     5281 tccctgtaca aatcggggca atggtatggg caaagaaaac ctccaaggcc tcaatatcct
     5341 ccccaatcat cttaagcagg ctaccgctgt cctgccggtc caatttccca ggagccaagg
     5401 cccgcaattt tgcgaaaatc aagcgacggt aagccgccag cgtatgaaag gccacataat
     5461 gaccaaagta atgctcacca tagcggaagg caccacgtaa aagagctagg agaatgagga
     5521 ttcccaaggt ccatagcgga gcaggattgc cagtaagggc attgaaagcc agatagacca
     5581 gcataactgg aatagcaatg gtcaccacct gtcccaagac cgcaaagcaa accgcaaccg
     5641 caatccgtgg caagaggtgc ttcatggact gcaagagtct cggaatcagc actcgtgttg
     5701 gcatttcttt cttatccata aactgcttcc tccaattctc tttgttgatc gaccagttga
     5761 ccgtaagcca agctagtctg ataaagcgtg gcatagttgc ctgtcacagc tccctccgca
     5821 gacaggaaga ggatgtcatc tgcctgctcc acctgtttca tcttatgggt tatgatgatg
     5881 acaatcgcat ccttggccac caagtcaatg aggtcgtaaa tcaagccttc attgtcctga
     5941 tcgacgctgg acgtcacctc gtcaaagata taaagggagc gtttagccaa gaccgcacgg
     6001 gcacagataa cctgctggcg ttgaccaggc gacaagaaag caccgtcatc acccacaatc
     6061 gtatccaaac cgtctggcag attcttgaca aactgcaaga ccccatgctg atctgcccaa
     6121 gccaagatgt cctccttgga ccagtcacaa gccatccgca agttatggta gatagactgg
     6181 ttaagcaagg ttgactggcc cgacacatag aggacctgct cattcagaga aagcttactg
     6241 atacctgaaa tctcctgctc acccaagtaa atagctccct tatccgcccg caagcgtccc
     6301 aagagcagct gggccaaggt cgttttcccc tgccctgact cgccggccaa agcatatacc
     6361 ttaccagccg gcatggtcat ggaaatatct tctaggaccg gcttttctcc gtaggcaaag
     6421 gccaaatctt ctagttttag actgtcaaaa gcaggcacat ggacagaatc gatttcctgc
     6481 tccgctgtca tactgtccaa gaaaccaaaa atgcgatccg ccatcttggt attcatcatc
     6541 accaagtgca taccgtagcc ctgttcccgt atgggtgcaa aaaactccgt cgcaatgagg
     6601 acaaagaaga gcatactaaa gagggacaaa ttgcctgctg ctaggctatt gaccgccaca
     6661 aaaccagaca agccaatccc cagatacatg accgcatcca tgtagcccac cgcttgcaac
     6721 tgaaagctga gcaactccat ggtcgcatcg cggaagtcct cggcctgttc attgaaggtc
     6781 ttttcgtagg tggcatccgc ctgataagaa tagagggtgt tgaggccctt gaggtcatcc
     6841 aggaaaagat tgcccacatc catataggaa ccccaatagc gattcatgat ccgcttggac
     6901 cgcttctgca tagcgataat ggaaatggga atcagaggca aggccaagat gaaaatgact
     6961 gcacccagcg ggaaaatcag aaaaaccagc aagagaaccg tcgcacagtt gaactgggtc
     7021 cgcaaggacg aagccatgta gtaggaatag taggtatcca ggctatcaat cccctgagaa
     7081 gcgatgttaa aaacatccgc cgccgtcgct ttggacgcaa actgaccatc cttggccaaa
     7141 agaacctcaa agaaagactg cttgagcgaa tcccgtgcaa actgggagcc cagcccctgc
     7201 aaacgcttgg caatcagagc cacaccatag ccaaaagcat tgagccccag caagatgagg
     7261 acaaagcgta ccagatcaac ctgaccaccc tcatagtagc tcacaaagcc cttagcaatc
     7321 agataaaagg aaataatccg catcaaaaat tgcacccatg atagaaaggc tgccaaataa
     7381 accagcttca tcttgggttt gatgcgttct ttgagccgct tcaagagggc ttttttcttt
     7441 tctctggaga ttttctcctg ctcttgtgcc atcatccacc tca
//
LOCUS       DBNFCK010000094         7353 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.094, whole genome shotgun sequence.
ACCESSION   DBNFCK010000094 DBNFCK010000000
VERSION     DBNFCK010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7353
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.094"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            213..764
                     /locus_tag="AC1PIW_001497"
     CDS             213..764
                     /locus_tag="AC1PIW_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HIP6606424.1"
                     /translation="MIVFLVICVFLVRIYFLKISIKNEKRILKNGGSEFGVQNTKLLT
                     IVHILFYLSCLVEAVVRHTKFDTLSSLGLIFLLFSMGMLFWVTRLLGDIWTVKLMLVK
                     DHRFVDHWLFRVVKHPNYFLNIIPELIGLALLCHATYSFLILFPVYMVILYRRIHEEN
                     NLLKTVIIPNGTVQSAAEITGNN"
     gene            complement(973..1881)
                     /gene="ypfJ"
                     /locus_tag="AC1PIW_001498"
     CDS             complement(973..1881)
                     /gene="ypfJ"
                     /locus_tag="AC1PIW_001498"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_02809 family neutral zinc metallopeptidase"
                     /protein_id="HIP6606425.1"
                     /translation="MKIDDLRKSSNIEDRRGQSSSNSSFSSGSSGGNVLLGLIFSRLG
                     WKGKLLVLVLLVAFGGMSNLGGLLSPSTGTNPYQSSQVTTTGTDVSDADAEFMSKVLG
                     STEDFWVQEFKANGLTYNAPTMVFYTNQTQTGCGVGSAQAGPFYCSADRKIYIDTSFY
                     QELSSKYKAAGDFAMAYVIAHEVGHHVQNELEVLGAYHQKRAQLSDKEGNALNVRLEL
                     QADYYAGAWAKYVDGQGILDAGDIDEAMNAAHAVGDDTLQQEAYGRTVPDSFTHGTSE
                     QRKKWFNLGYTYGDLAHANTFSVEKP"
     gene            2049..3392
                     /gene="xseA"
                     /locus_tag="AC1PIW_001499"
     CDS             2049..3392
                     /gene="xseA"
                     /locus_tag="AC1PIW_001499"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIP6606426.1"
                     /translation="MVEYLSVSHLTKYLKLKFDRDPYLEKVYLTGQVSNFRKRPSHQY
                     FSLKDDKAVIQVTMWAGVYKNLGFELEEGMKINVIGRIQLYEPNGSYSIVIEKAEPDG
                     IGALAIKFEQLKQALTQEGLFKEEFKQALPRFTKKIGVITSPSGAVIQDIITTVSRRF
                     PGVEIVLYPTKVQGDGAAQEVVTNIQRANERDDLDVLIVGRGGGSIEDLWAFNEEIVV
                     RAIFESRIPIISSVGHETDTTLADFVADKRAATPTAAAELATPVTKADLLGYLNQQEN
                     RAYQAMTNRLRFLRGQLEKISQSVMFRQPERLYDGYLQKLDRLTNQLQTRMKELYSQQ
                     KQACLLLQQRLQGVHPGRKVESFQERIIQQERLLKSNMANIYDNKMAKADKLIEALTM
                     LDTSRIVARGYAMIRQDGRVIDSVENVQMGENLTIQMKDGQLDVEVKHVQTDENI"
     gene            3367..3582
                     /locus_tag="AC1PIW_001500"
     CDS             3367..3582
                     /locus_tag="AC1PIW_001500"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIP6606427.1"
                     /translation="MSKQTKTFEENLAELEGIVTKLERGDVALEEALAEFQKGMVLSK
                     DLQKTLAEAEKTLVKVMQADGSEAEMD"
     gene            3582..4451
                     /locus_tag="AC1PIW_001501"
     CDS             3582..4451
                     /locus_tag="AC1PIW_001501"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIP6606428.1"
                     /translation="MVTSKLQRLEEAMADFYQPQVAERLNQSVLYSLQAGGKRIRPLL
                     LLTILESFGMELKQAHYQVAACVELIHTGSLIHDDLPAMDNDDYRRGRLTNHKVYDEA
                     TAILAGDSLFLDPFGFLAQVDLPAETILALVRELSQASGTFGMVAGQVLDMAGEGQNL
                     TLEQLQAIHANKTGKLLTFPFVAAGLIAGQSDVVINHLRQAGQLIGLAFQVRDDILDV
                     TATFEEIGKTPQKDVLAEKSTYPALMGLEASKVFLEDSLNQAINELDQVAKQVPFSTE
                     KIKEIIESLRING"
     gene            4444..5265
                     /locus_tag="AC1PIW_001502"
     CDS             4444..5265
                     /locus_tag="AC1PIW_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HIP6606429.1"
                     /translation="MAKERVDVLAFKQGLFETREQAKRGVMAGLVISVINGERYDKPG
                     EKIDEGIELKLKGEKLKYVSRGGLKLEKALQVFHLSVEGQTTIDIGASTGGFTDVMLQ
                     AGAKQVYAVDVGTNQLAWKLRQDERVISMEQYNFRYAELGDFELGQPSFASIDVSFIS
                     LSLILPALHRILAEGGQVVALVKPQFEAGREQIGKNGIVKDKSVHLKVLEEVTKMAVE
                     TGFSVKALSFSPVQGGHGNIEFLAHLEKSTQPNQIDKEVITTTVYQAHEEFKKDE"
     gene            5258..5683
                     /locus_tag="AC1PIW_001503"
     CDS             5258..5683
                     /locus_tag="AC1PIW_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HIP6606430.1"
                     /translation="MNKRERLEVIKDLVVRFPIDTQEEIVERLEAMGVNATQATVSRD
                     IKELGIIKVPSADKGYIYGLPKAGLVKVKSQNVFEVSVMDKMINLRLVPGSSAVVKRQ
                     ILEQFQEHVFSVIADDDSILLIVTNEGIISQIEQTVRGW"
     gene            5686..7347
                     /gene="recN"
                     /locus_tag="AC1PIW_001504"
     CDS             5686..7347
                     /gene="recN"
                     /locus_tag="AC1PIW_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIP6606431.1"
                     /translation="MLLEVSIKNFAIIEQVSLNFENGMTILSGETGAGKSIIIDAMNL
                     MLGARATTDVIRHGAAKAEIEGLFSFENSRALEQILLEQGIEVADELIIRREILQNGR
                     SVSRVNGQMVNLSVLKQIGQYLVDIHGQHDQEELMKSQHHIRLLDSFGEDEFWSLKDR
                     YQTTFDAYRSLRKRVLEKQKNEQEHKARIEMLEYQIAEIEAADLKSGEDIQLNQERDK
                     LLNHKQIADTLTNAYALLDNEDFSSLNNLRSAMSDLQSLEEFDPDYKQLSSSLTEAYY
                     VVEDITKRLSDVVDNLDFDGNRLMQLESRLDLLNTITKKYGGTVDDVLDYFSKISEEY
                     NLLTGNDLSGDDLEVQLKNLEKELVERAGQLSQSRHELAVVLEDIIRQELQDLYMEKA
                     RFQVRFTKGKFNREGNETVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFARKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIYKIGQYGQVLAISHLPQVIAIADYQFFIEKIS
                     DEHSTVSRVRLLTKEERIEEIAKMLAGDKITDAARNQAKELVEKG"
BASE COUNT         2250 a         1371 c         1691 g         2041 t
ORIGIN      
        1 gtacaagctg tctgcagctc gttgactggt actaaaagta aactaaaaga ctataaaaga
       61 gacatgattt catccatttt tgaaaacagg tctcttttat tgtagtctca aatggaaaat
      121 agaaaaaaaa caggagagga atagaatggc gacagtctaa ctttgtataa tggatgtgtt
      181 tgataaaatc tgttaaaata caggagggat ttatgattgt ttttttggtt atttgtgttt
      241 ttttggtgag aatctatttt ttgaagattt caattaaaaa cgaaaaacgg attttgaaaa
      301 acggcggttc agaatttggt gttcaaaata ctaaattatt aacaattgta catattctat
      361 tttatttatc atgtttggtt gaagcagtag tgcgacacac caagtttgat acgcttagta
      421 gcttaggttt gatattcttg ctgttttcaa tgggaatgtt attttgggta acacgattgt
      481 taggtgatat ttggacagtt aagctcatgt tggtaaaaga tcatcgtttt gttgatcatt
      541 ggttgtttcg tgttgtaaag catcctaact atttcctaaa tattatacca gaattaatcg
      601 gtttagcttt attgtgtcat gcaacatata gttttctcat cttattccca gtctacatgg
      661 ttatcttata cagacgtatt cacgaggaaa ataatttgtt gaaaacagtg attattccaa
      721 acggaactgt gcagtctgca gcagagataa ctggaaataa ttaaagtttg ctaaataata
      781 attgtgatat tgtttgaaac taggataccg ctctaattag agttgatgag taagtttaca
      841 tgattcagtt ttcatattta aaagtggtac cgtatgtgta aattggcttt tgtcagttgt
      901 atattataaa gaaactcttg caaaagtgta tgttgcaaga gtttctttat tgctacgaga
      961 ggctatggat gtttacggtt tctctacgga gaatgtattg gcgtgagcaa ggtcaccata
     1021 ggtataaccg agattaaacc attttttcct ttgctcagaa gtgccgtggg taaaactgtc
     1081 tggaacagta cggccgtagg cttcctgttg aagagtatcg tcaccaactg catgggcagc
     1141 attcatggct tcgtcaatat caccagcatc caagatgcct tgtccatcaa catatttggc
     1201 ccaggctcct gcataatagt cagcttgcaa ttctaaacga acatttagcg cattgccttc
     1261 cttatcggat agttgagccc gtttttgatg gtaggcccca agaacttcta attcattctg
     1321 aacatggtga ccaacttcat gagcgatgac ataggccatg gcaaagtcac cagcagcctt
     1381 atacttgcta gataattctt gatagaaaga agtatcaata taaatcttgc ggtcagcaga
     1441 gcaatagaat ggaccggctt gggcggaacc aactccacat ccggtttggg tctggttagt
     1501 atagaaaacc atagtcggtg cattgtaggt taaaccattg gctttaaatt cttggaccca
     1561 aaaatcttct gtcgatccta aaaccttact catgaattca gcatctgcat cagacacatc
     1621 tgtaccagtc gttgtgactt gactcgattg gtaaggatta gtccctgttg atggggaaag
     1681 caacccacca aggtttgaca tgcctccgaa ggctactaaa aggacaagaa caagtagttt
     1741 tcctttccaa ccaagtcgtg aaaagattag tcccaataga acatttccgc cagatgaacc
     1801 agacgagaaa gatgagttag aactggattg tcccctgcgg tcctcaatgt tagaactttt
     1861 tcgcaaatca tcaattttca tataaatctc cttagaacct gtcaacaagt tcttattttc
     1921 tcacaaacta ttatatagca aattgttgaa aaaaaacata ggaaagctat cggaaataat
     1981 aggcctagtt ctgtataaaa gtgcaaaatt tggtataata gtagggatac aatatagaag
     2041 gagtggacat ggttgaatat ttatcggtta gtcatctaac taagtattta aaattaaagt
     2101 ttgaccgcga tccgtatttg gagaaggtgt atttgacagg tcaagtgtcc aacttccgta
     2161 aacgccctag ccatcaatat ttttcgctca aggatgacaa ggcagtcatc caagtgacca
     2221 tgtgggcagg cgtctataag aatctgggct ttgagcttga agagggaatg aaaatcaatg
     2281 tcattgggcg cattcagctc tatgaaccaa atggttctta ttctattgtc attgaaaaag
     2341 ctgagccaga tggtattgga gccttggcaa tcaagtttga acagctcaaa caagctctga
     2401 cacaggaagg cttatttaaa gaagaattca agcaggcctt gccacggttt accaagaaaa
     2461 tcggtgtcat taccagtccg agtggagctg ttattcagga tattattaca accgttagcc
     2521 gacgttttcc aggcgtagag attgttctct atccgaccaa ggtgcaagga gatggtgctg
     2581 ctcaggaggt tgttactaat attcaaagag ccaatgaacg ggatgacttg gatgttctga
     2641 ttgttggtcg tggcggtggt tctatcgaag acctttgggc tttcaatgaa gaaattgtgg
     2701 ttcgggctat ttttgaatcc cgtattccaa tcatttccag cgtaggtcat gaaacagata
     2761 cgaccttggc tgattttgta gcagataaac gagctgctac cccaacagct gccgcagaat
     2821 tagcaacacc tgtgaccaag gcggacttac tgggctacct caatcagcag gaaaatagag
     2881 cctatcaggc catgaccaat cgtttgagat tcctaagagg tcagcttgag aaaattagcc
     2941 agtcagtcat gttccgccag cctgaacgct tgtatgatgg ttatttgcaa aaattagacc
     3001 gcttgaccaa tcaattgcag acacggatga aagaactcta cagccagcag aaacaggctt
     3061 gtctcctact acagcaacga ttacaaggtg tgcatccagg tcgtaaagta gaaagtttcc
     3121 aagagagaat tatccagcag gaacgactct tgaagagcaa tatggctaat atctatgaca
     3181 ataagatggc caaggcggac aagctgattg aggccctgac tatgctggat accagtcgca
     3241 tcgtagcccg tggctacgcc atgattcgtc aagatggtcg ggtgatcgat agtgtagaaa
     3301 atgtacaaat gggtgaaaac ctaaccatcc agatgaagga tggtcaactt gatgtggagg
     3361 taaaacatgt ccaaacagac gaaaacattt gaagaaaact tagctgaatt agaaggaatc
     3421 gtaaccaaat tggagcgagg cgatgtggct ctggaagaag cactggccga gtttcaaaaa
     3481 ggaatggttc tatcgaaaga cctacaaaag acactagcag aagctgaaaa gaccttggtc
     3541 aaggtcatgc aggcggatgg cagtgaagcg gagatggatt aatggttacc agtaaattgc
     3601 agcgacttga ggaagccatg gcagactttt accaacccca ggtagctgaa cgcctcaacc
     3661 aatcggttct ttattcgcta caggctggtg gtaaacgaat ccgccccttg cttttattga
     3721 ccatcttaga gtcctttggg atggaattaa aacaagctca ttaccaagtg gcagcatgtg
     3781 tcgagttgat ccatacggga agtttgattc acgatgacct gcctgctatg gataatgatg
     3841 attaccgtcg tggtcgcttg accaaccaca aggtttacga tgaagcaacg gctattttag
     3901 caggggacag tctctttcta gatccatttg gtttcctagc ccaagttgat ttaccagctg
     3961 agaccatcct tgctctcgtg agagagcttt cccaggcatc tgggaccttt ggaatggttg
     4021 ctggtcaagt cttggatatg gctggtgaag ggcagaattt gaccttagaa caattacaag
     4081 ccatccatgc caataagact ggaaaattat tgacatttcc ctttgtagca gcaggtttga
     4141 ttgcaggaca atcagatgtg gtaatcaacc atttgcgaca agcgggtcaa ttaattggtc
     4201 tagcctttca agttcgggat gatattttgg atgtgacagc cacttttgag gaaatcggaa
     4261 aaactcccca aaaagatgta ttggctgaga agtctaccta ccctgcccta atgggactgg
     4321 aagcctcaaa agtctttcta gaagatagct tgaatcaagc aatcaacgag ctagatcaag
     4381 tggctaaaca agtcccattt tcaacagaaa aaatcaaaga aatcatagaa agtttacgaa
     4441 tcaatggcta aggaaagagt agatgtacta gcttttaaac aggggctttt cgagacgcgg
     4501 gagcaggcca aacgcggtgt tatggctggc ttagttatat ccgtcattaa cggggaacgc
     4561 tacgataaac caggtgagaa gattgacgag ggaattgaac tcaagctcaa gggggaaaaa
     4621 ctcaagtatg tcagccgtgg tggtctcaaa ttggagaaag ccttgcaggt tttccactta
     4681 tcagttgagg gtcaaacaac gattgatatt ggggcttcaa caggcggatt tacggatgtt
     4741 atgttgcagg ctggtgccaa gcaggtctat gctgtggatg tagggaccaa ccaactcgct
     4801 tggaaattac gtcaggacga acgtgtaatc agcatggaac agtacaattt ccgctatgct
     4861 gagttgggag atttcgaact tggtcaacca agttttgcgt ccattgacgt cagctttatc
     4921 tcattgagtc tgattttgcc agcactccat cgtattttag cggagggtgg ccaggttgtt
     4981 gcccttgtca aaccccaatt cgaggctgga cgtgaacaga ttgggaaaaa tggcattgtt
     5041 aaggataaat ccgttcactt gaaagtcttg gaagaagtga caaaaatggc cgtggaaaca
     5101 ggcttctcag tcaaagccct tagcttttcc cctgttcaag gcggtcatgg caatatcgaa
     5161 tttttagcgc acttagaaaa atcaacacaa ccaaaccaga ttgataaaga ggtcataaca
     5221 actacagtct atcaagcaca tgaggaattt aaaaaggatg aataaacgag aacgattaga
     5281 agtcattaaa gatttggtag tccgatttcc tattgataca caggaagaaa ttgttgaacg
     5341 tctagaagca atgggtgtca atgcgacaca agcaacggtt tcacgtgata tcaaggaact
     5401 tggcattatt aaggttccat cagcagataa gggatatatt tatggtttac caaaggcagg
     5461 tcttgttaag gtaaaatcgc agaatgtttt tgaagtctca gtgatggata aaatgattaa
     5521 tctccgtctg gtacctggaa gctctgctgt ggtcaaacgc cagattttgg aacaatttca
     5581 agaacatgtt ttttcagtga ttgctgatga tgacagtatt ctgctcattg ttacaaacga
     5641 aggtattatt tcgcaaatcg aacagacggt tagagggtgg tagccatgtt actagaagtg
     5701 tccattaaaa acttcgccat tattgagcag gtatcactga attttgaaaa cgggatgacc
     5761 atcctatctg gtgaaacagg tgcaggtaaa tccattatca ttgatgccat gaacctgatg
     5821 ctaggggcgc gtgcgacgac agatgttatc agacatgggg ccgccaaggc tgagattgaa
     5881 gggctctttt cctttgaaaa tagcagagct ctcgagcaaa ttttgcttga acaggggatt
     5941 gaagtagctg acgaactcat tatccgccgt gaaatcctgc aaaatggccg ttcggtcagc
     6001 cgtgtcaatg gacaaatggt caatttatct gtcttgaaac agattgggca atatttagta
     6061 gatattcatg gtcaacatga ccaggaagaa ttgatgaaat cccagcacca tatccgtctt
     6121 ttggacagtt tcggagaaga tgaattttgg agtcttaaag accgttatca gacaaccttt
     6181 gatgcctatc gtagtcttcg caaacgagtt cttgaaaagc aaaaaaatga gcaagaacac
     6241 aaggcgcgga ttgagatgct agaataccaa attgctgaaa tcgaagcagc ggatttgaag
     6301 tctggggaag atattcaact caatcaggaa cgcgataaac tgctcaacca caaacaaatt
     6361 gcagatacac tgaccaatgc ctatgcactg ttagacaatg aagatttttc aagcttgaac
     6421 aacctacgct cagctatgag tgacttgcaa agtctggaag aatttgatcc agactacaaa
     6481 cagctctctt ctagcctgac agaagcttat tatgtcgttg aagatataac caagcgtctc
     6541 agcgatgtgg tcgataatct agattttgac ggcaatcgtc tcatgcaatt ggaaagtcgt
     6601 ctggatttgc tcaataccat tacgaagaaa tatggtggaa ctgtcgatga tgttttggac
     6661 tattttagta aaatcagcga agaatacaat ctattgacag gcaatgattt atctggagat
     6721 gatttggaag ttcagcttaa gaacttagag aaagaattgg ttgaacgagc aggtcagctc
     6781 agccaatcac gccatgaatt ggctgttgtt ttagaagata ttatccgcca agaattgcaa
     6841 gacttgtata tggagaaagc tcgtttccaa gttcgattta ctaaaggaaa atttaatcga
     6901 gaaggaaatg aaacagtaga attttacatc tctaccaacc caggtgaaga cttcaagcct
     6961 ctggttaagg tggcttctgg aggagaattg tctcgcttga tgttggccat taagtcagcc
     7021 tttgcccgta aagaaggcaa gacttccatc gtctttgacg aggtggatac aggggtgtct
     7081 ggacgtgtgg cccaggccat tgcccagaaa atttataaaa tcggacaata tgggcaagta
     7141 ttggcgattt ctcatctacc acaagtcatt gctattgcag attatcagtt ctttattgag
     7201 aaaatatccg acgaacattc gaccgtttct cgtgtccgtc tcttgaccaa agaagagcgt
     7261 attgaggaaa ttgccaagat gttagccggt gacaaaatca cagatgcagc ccgtaatcaa
     7321 gcaaaagaat tagtagaaaa aggttaggcg act
//
LOCUS       DBNFCK010000095         7221 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.095, whole genome shotgun sequence.
ACCESSION   DBNFCK010000095 DBNFCK010000000
VERSION     DBNFCK010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7221
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.095"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..1299
                     /locus_tag="AC1PIW_001505"
     CDS             <1..1299
                     /locus_tag="AC1PIW_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HIP6606432.1"
                     /translation="KLAPFGTKVGNQRHLKAVRDGFMMAMPLVLVGSFFLLLISWPDQ
                     ASGGFSVVGFLEQIGLLGILNRLNQSTMAIISLIATFGIAYRLSESYETDGPSAGVIA
                     LSSFILLAPRFSTMVFNESGEQINQVFAGAIPFGSLNASALFVAIAVGLVSAEIYRFF
                     IQRGITIKMPKNVPDVVGKSFAALLPALTTLIFWACVFQGLEAAGVEGGLGAVLGLIV
                     GKPLGLIAGSLGGMVLVVLVNSLFWFTGVNGGQVLNAFVDPVWLQFTEANQLAAAAGE
                     KLPHIITLPFKDLFVFMGGGGATVGLALCLLVFSKSRTFKSLGRISFIPSLFNINTAI
                     LFSFPTVLNPIMMIPFILTPTVNAIITYFAMATGLVPMTTGVVLPWTMPPILGGFLAT
                     GGSIQGAILQIILIGVSFIIYYPFFKVADNKNLELEESGE"
     gene            1304..3190
                     /locus_tag="AC1PIW_001506"
     CDS             1304..3190
                     /locus_tag="AC1PIW_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HIP6606433.1"
                     /translation="MKLTIFDKVQNKTSQFAIDELTYYLKAIFDFELGKTTVEEDANV
                     LLSKEYLDNNDTIGIEMKNGNAILTGNSDVSILIAVYRLLSEFGAVFTRPGRKHELIP
                     SLTITDWNEKELSIHETASYKHRGVCIEGADSYQNTKEFLDWLPKINMNSFFIQFENP
                     YSFLKRWYEHEFNPYADKEDFNTEIAQQMSDLLDEELSKRGLIHHRVGHGWTGEVLGY
                     SSKYGWESGLKLPEEKKPLVAELNGKRELFNTAPILTSLCFSNPDVGERMCELIVNYA
                     KERKDVDYLHVWLSDARNNICECEKCKQEIPSDQYVRILNQLDRKLSEEGLNTKICFL
                     LYHELLFAPKHEVLENPERFTMMFAPITRTFEMSYADVDYENDVPLPNEYSRNNIVLP
                     NSLEENLSYLFSWQKVFNGDSFVYDYPLGRAHYGDLGYMKISKVIFRDVVYLKKLGLN
                     GYISCQELRAGFPHNFPNFVMGQMLWNRNLEYDELKALYFSSLYGSEWKSVVTYLEKL
                     SDYSSCDYFNSIGDRQNAELSMKYNISSKLAFNFINTLEENVAKNNGIQRDAWRQLGY
                     HREYVVKLANALHLMASGDHLDGQIRWKDFLDYIRNHEPDFQQYLDVYRIIEVAKNYA
                     GFKL"
     gene            3310..7026
                     /locus_tag="AC1PIW_001507"
     CDS             3310..7026
                     /locus_tag="AC1PIW_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HIP6606434.1"
                     /translation="MNKKIFLKSAIILLSVTCLINAIKALDSSGVSSSTGEIVNQDRI
                     YNITLKVKTDGVYNDLGGNVISENNVLSGISGTKINLKVSTNSGYRFVGYQSFLESGN
                     STDGLLPIENDSFEISDKTGNVTVVAMFEKIPIDETIYFSDEFSTENINNYLLSENLI
                     GKIAVSNGKLNIHAPGVSSIKPILSRQIDESSTLGYEIQFSIQQIGEVKQWNTFRVVF
                     KENSDGSVYALEFTGKAVSIKKLSSIDAPNQTGEKYAETGHNLGSEEHRIRLVVRGDT
                     VTVSDNEIPLLSYSSPENWEGATASIVFTPISNRSVSLDDIIIRQTRALRSLLVVSRI
                     DGQEVTDIQPGSIRGNTSQVFVGDSLPLEVIEKPGYQFIGFKDEFGNVVDLSTFSVPN
                     DESDLVIYADFQTAEVVNRETKTFYIDSIEGNDTNSGESETNAWKTLEQLRKNTLIAG
                     DRVLLKRGSRFVGEDAALTFKGSGLEDAPILISSYGEGELPLLEAQGKIESVIKLYNQ
                     EYITIENLEITNLDPNFSTSFELNSNNNRSKILRGVHVIAEDYGVVHDIVLRNMYLHD
                     INGNLNSKWNGGIFFDVYGTTVPTKYDGILIENNYLERVDRSGIKLVGSTWANQNLKN
                     NKNIPLNWYPSTNVVVRGNRIEKAGGDSITVRDTDGALIEYNISADARYQDTGYNAGI
                     WPFQASNTVIQYNESFRTHGVQDGQGLDLDHVSNNSVMQYNYSHDNEGGFMLIMNWYE
                     QTSPTVRYNISQNDKDKIFELARGGAQGTAIYNNTIFSDSKLTGRAGVIDMPSTSGGT
                     GVKDIFLFNNIFYFTDGEKMFVEASDAGKYKDSIHFYNNAYVGVEVPDDPKAITEGIT
                     LKGVGTGPTENKSMIANAGKFLTGQLDGYRLPENSSLAKLGVSKEEAISYFYKKLGVQ
                     PTIDFQDNGSLTMSPTEAFALARQTNSVDAIARVYPAIEGVTYDTDFFGETLSSENLS
                     VGAAQEKKSVFEEKISDIEISDVKTGIGMRYKRSNQLADVNLSIRLINKDELQYTINA
                     NNPTFSYEIQLQKNFGEYMDLSESVTITIPIEADKKILNVLKINKNEQTESVKELPYR
                     IENNKIILETDELGSFIIEYQMEKIKKENNTNATISESGKSSLGLQNKENSDVQAVGD
                     KDYFSGLELSNKGEELSREDSKLMGNSINNLQTIKESESIFEGNLGRNKELSDMYQKS
                     LPKTGESNQAYIVLIGLFSLFVVLICQIFKKSID"
BASE COUNT         2415 a         1001 c         1462 g         2343 t
ORIGIN      
        1 aaactagctc cttttggaac taaggtaggg aatcaacgtc atttgaaagc agttcgtgac
       61 ggttttatga tggctatgcc tcttgtatta gttggctcgt tcttcctatt gctaatcagt
      121 tggccggatc aggcttctgg gggattttca gtagtaggct ttttagagca aattggtttg
      181 ttggggattt taaatcggtt aaatcaatca acaatggcga ttatctctct catagctaca
      241 ttcggtattg catatcgatt atccgaatca tatgagacag atggaccttc tgcaggtgtt
      301 attgctttat catcttttat tttacttgcg ccaagattct caacaatggt tttcaacgaa
      361 tctggtgaac aaattaatca agtatttgct ggagcaattc catttggaag tttgaacgca
      421 agtgcattat ttgttgctat tgctgttgga ttagtttcag ctgaaattta tcgtttcttt
      481 atccaaagag gtattacaat taaaatgcca aaaaatgtcc cagacgttgt gggtaagtca
      541 tttgccgcat tgcttccagc attaacaaca ttaattttct gggcttgcgt cttccaggga
      601 ttagaagctg ctggtgttga aggtggactt ggagcggttt tgggattaat tgtaggtaaa
      661 ccacttggtc ttattgcagg aagtctcggt ggaatggtgc ttgttgtgct tgtaaattca
      721 cttttctggt ttactggtgt taatggtgga caagtgttaa atgcttttgt cgatccggtt
      781 tggttacagt ttacagaagc aaatcaactt gcagccgccg cgggagaaaa attaccacat
      841 attattactt tgcctttcaa agatttgttc gtatttatgg gcggtggtgg cgctactgta
      901 ggattagcgc tttgcttact tgtctttagt aaaagtcgta catttaagag tttaggacga
      961 atctccttta ttccttctct ctttaatatt aatacagcca ttctgtttag ttttccaact
     1021 gttttgaatc ctattatgat gattccgttc attctaacac cgacagttaa tgcaataatt
     1081 acctattttg ctatggcaac tggattagtt ccgatgacta ctggggttgt tctcccttgg
     1141 acgatgcctc caattcttgg ggggttcctt gctactggcg gcagtattca gggagcaatt
     1201 ttacaaatta ttttaatagg tgtctcattc attatttatt atccattttt caaagtggcc
     1261 gataataaga atttagagct tgaagaatca ggagagtgat catatgaaat tgacgatttt
     1321 tgataaagta cagaataaga cttctcaatt tgctatagat gaacttacat attacttgaa
     1381 agccattttt gattttgagt taggaaagac aactgttgag gaagatgcga atgttctttt
     1441 gtcaaaagaa tatcttgaca ataatgatac gattgggatt gaaatgaaaa atggaaatgc
     1501 aatattaaca ggtaattctg atgtctcaat ccttattgcg gtatatcgtt tacttagtga
     1561 atttggagct gtattcacta gaccgggtag aaaacatgaa ttaatcccct cattaacgat
     1621 aactgactgg aatgagaaag agctttctat tcatgagact gcaagttata agcatcgagg
     1681 agtatgtata gaaggtgcag atagttacca gaatacaaaa gaatttttgg attggttacc
     1741 caaaattaat atgaatagct tttttattca gtttgagaat ccttattcat ttttaaagcg
     1801 ctggtatgaa catgaattta atccgtatgc ggataaagaa gatttcaata cggaaattgc
     1861 acaacaaatg agcgaccttt tagatgaaga attatcaaaa cgaggtttaa tacatcatcg
     1921 tgttggccat ggttggactg gtgaagtact tggatattca tctaagtacg gatgggaatc
     1981 tgggttaaaa ctacctgaag aaaagaaacc gctagtcgcc gaattaaatg gaaaacgtga
     2041 gttattcaac acagcgccaa tactaactag tctatgtttc tccaatccag atgttggtga
     2101 aagaatgtgt gaactgattg taaattatgc taaggaaagg aaagatgtag actatttaca
     2161 tgtttggtta tccgatgcac ggaacaatat ttgtgaatgt gaaaaatgta aacaagaaat
     2221 accctcagat caatatgtac gaatattaaa tcaattagat cggaaattaa gtgaagaggg
     2281 gcttaataca aagatttgtt ttttacttta tcatgaatta ctttttgcac caaaacatga
     2341 agttttagag aatcctgaac ggttcactat gatgtttgca ccaattacca gaactttcga
     2401 aatgagttat gcagatgtag actatgaaaa cgatgttccg cttccaaatg agtattcaag
     2461 aaataatatt gttcttccta actcacttga agaaaatctc tcatatttat ttagttggca
     2521 aaaagtgttt aatggagata gttttgtata tgattaccca cttggtcggg cacattatgg
     2581 tgatttagga tatatgaaga tcagtaaagt aatctttaga gatgtcgtat atcttaaaaa
     2641 attgggttta aatggttata ttagttgcca ggagctacgc gctggtttcc ctcataactt
     2701 tcccaacttt gtgatgggtc aaatgttgtg gaacagaaat ttagagtacg atgaattgaa
     2761 agcgctctat tttagttcct tatatggttc tgaatggaaa agtgtcgtaa cttacttaga
     2821 aaaattatca gattactctt catgtgatta ttttaattca ataggtgatc gccaaaatgc
     2881 agaattatca atgaaataca atatctcaag taaattagca tttaatttta ttaatacctt
     2941 ggaagagaat gttgctaaaa ataacggaat tcaacgtgat gcttggcgac aattaggtta
     3001 tcaccgggaa tatgttgtca aattggctaa tgcccttcat cttatggcct ctggagacca
     3061 tcttgatgga caaatccgct ggaaagattt tttggattat attagaaacc atgaacctga
     3121 ttttcaacag tatcttgatg tctatcgtat tattgaagtt gctaagaatt atgctggatt
     3181 caaattgtag tactgagttg tagaaaaaag ccaattcaca atttattttg cgaattggct
     3241 tgaagtctaa tatagataga atattgaatt tatttgatgt taattgatat ttatttttga
     3301 gaggatttta tgaataaaaa aattttttta aaatcagcga ttattttgct ctctgtcaca
     3361 tgtctaatta atgcgattaa agctttagat agtagtggtg tgtcatcgag tacaggtgaa
     3421 atagtaaatc aagatcgcat atacaacata actttgaaag tgaaaacaga tggggtttat
     3481 aatgatctgg gggggaatgt tatttcggaa aataatgtat tgtcaggaat tagtggtaca
     3541 aaaataaatt tgaaggttag tacaaattca ggatatcgtt ttgtagggta tcagtcattt
     3601 ttagaaagtg gaaatagtac agatgggtta ctaccaatcg aaaatgatag ttttgaaatc
     3661 tcggataaaa cgggtaatgt aacggttgtt gcgatgtttg agaagattcc tattgatgag
     3721 acaatttatt tttcggatga attctcaact gaaaatatta ataattactt gttatctgaa
     3781 aatctaattg gaaaaattgc ggtaagtaat ggaaaattga acattcatgc tcccggggta
     3841 tcatcgataa aaccaattct ttcaagacag attgatgaaa gttcaacatt ggggtatgaa
     3901 attcaatttt ccatacaaca aattggtgaa gtaaagcagt ggaatacttt tagagtagta
     3961 tttaaagaaa atagcgatgg atcagtttat gctctagaat ttactggaaa agctgtatct
     4021 ataaaaaaac tttcaagtat tgatgcacct aatcaaactg gtgaaaaata cgctgaaaca
     4081 ggccacaatc taggaagtga agaacacaga ataagacttg tagtaagagg ggatacagta
     4141 actgttagtg ataatgaaat tccgttatta agttatagca gtcctgaaaa ttgggaaggt
     4201 gcaacagcat ccattgtttt tacaccgatt tcaaatagaa gtgtatcttt agacgatatc
     4261 attattcgac aaacacgtgc tttaagatct ttactagttg tgtctcgtat agatggtcag
     4321 gaagttacag atattcaacc cggatcaatc agagggaata caagtcaagt ttttgttgga
     4381 gattcacttc ctctggaggt cattgaaaaa cctggatatc aatttattgg ttttaaagat
     4441 gaatttggaa atgtggtcga tttatctact ttcagtgttc ctaatgatga gtcggatttg
     4501 gttatttatg cagactttca gacagctgaa gtagtgaatc gtgagacaaa gactttttat
     4561 attgatagta tagaaggaaa tgatactaat agtggagaaa gtgagactaa tgcatggaaa
     4621 acactcgaac aattgcggaa aaacacacta attgctggtg atagagtgct attaaaacgt
     4681 ggaagtcgtt ttgttggaga agatgcggca ttaactttta aaggttcagg tcttgaagat
     4741 gcgcctatcc taatttcatc ttatggtgag ggagagctgc ctttgcttga agcacagggc
     4801 aaaattgaaa gtgtaattaa gttatataat caagaatata ttacaattga aaatctagag
     4861 attacaaact tagatccgaa cttttcaacg agttttgaat taaattcaaa taataaccgt
     4921 tcaaaaatac taagaggtgt tcatgttata gctgaagatt atggggtagt tcatgatatc
     4981 gttcttcgaa atatgtatct acatgatatt aatggtaatt tgaattcaaa atggaatgga
     5041 ggtattttct ttgatgttta tggaacaaca gttccaacca aatatgatgg catacttatt
     5101 gaaaataatt atttagaaag agttgatagg tcagggatta aattggtcgg gtccacatgg
     5161 gcaaaccaaa atctaaaaaa caacaaaaac attccattaa attggtatcc atcaacaaac
     5221 gtagttgttc gtggcaatcg tatcgaaaag gctggtggtg attctataac agttagagat
     5281 acagatggtg ctttaattga atacaatatc tcggctgatg ccagatacca agatacaggt
     5341 tataacgctg gaatttggcc atttcaagct tcaaatactg ttattcaata taatgaatca
     5401 tttagaaccc atggtgttca ggatgggcaa ggtttagatc tagaccatgt ttcaaataat
     5461 tcagtaatgc aatataatta cagtcatgat aatgaaggtg gtttcatgtt gattatgaat
     5521 tggtacgagc aaacatctcc aacagtccgt tataatatta gtcagaatga taaagataaa
     5581 atttttgaat tggctagagg gggagcccaa ggaactgcta tatataataa taccatcttc
     5641 tccgactcga aattaacagg gagagctggc gttattgata tgccgagtac ctcaggagga
     5701 actggtgtaa aagatatctt tttatttaac aatatttttt actttactga tggcgaaaaa
     5761 atgtttgtag aagcaagcga tgctggtaaa tataaagata gtattcattt ttataacaat
     5821 gcatatgtcg gagttgaagt gccagatgat cccaaagcga taactgaagg tataacttta
     5881 aaaggtgttg gaactggacc gactgaaaat aaatcgatga ttgcaaatgc tgggaaattt
     5941 ttaacaggac aattagatgg gtatagattg cctgaaaata gttcgttagc taagcttggt
     6001 gtgagcaaag aggaagcaat ttcttatttc tataaaaaac taggtgtgca gcccacaatt
     6061 gatttccaag ataatggctc cttaactatg tctccgacag aagcttttgc cttggctcgt
     6121 caaacaaaca gtgttgacgc gattgcacgt gtttatcctg caatagaagg ggttacttat
     6181 gatactgatt tctttggaga aactttgtcg agtgaaaatt tatctgttgg tgcagctcaa
     6241 gagaaaaaat cagtttttga agaaaaaatc tctgatatag aaatttcaga tgtgaaaaca
     6301 gggattggaa tgagatataa acgttcaaat cagcttgcag atgtaaattt aagtataaga
     6361 ttgataaata aagatgaact tcaatatact ataaatgcta ataatccaac tttttcatat
     6421 gaaattcaac ttcaaaaaaa ttttggagag tatatggatt taagtgagag tgtgacaatc
     6481 accattccaa ttgaggcgga taaaaagatt cttaatgttt tgaaaattaa taaaaatgaa
     6541 caaactgaat cagtgaaaga actgccatat cgaattgaaa ataataaaat aatattagaa
     6601 accgatgagc ttggttcatt tatcattgaa taccagatgg aaaaaattaa aaaagaaaat
     6661 aacacaaatg caacaataag tgaaagtgga aaaagtagtt tagggttgca aaataaagaa
     6721 aattcagatg tgcaagctgt aggcgataaa gattattttt ctggtcttga actatcaaat
     6781 aaaggtgaag agttaagtcg tgaagattct aaactaatgg ggaattcaat taataattta
     6841 caaacaataa aagagtctga aagtattttc gaaggaaatt tgggtaggaa taaagagttg
     6901 agtgacatgt atcaaaagtc attgccaaaa acaggagaaa gtaaccaagc atatattgtt
     6961 ttgattggtt tatttagcct cttcgtagtc ctaatttgtc aaatttttaa aaaatcaata
     7021 gattaaggga tttaggataa caaaatgacg aaaaaaataa tatttatctt gttattcttc
     7081 gtagtttgtg tttatttcta ctcctttatt cccctcgttc agactgttcc atctgttttt
     7141 cagacacctt atagaacatt agttcaatct gagtttgaag cgatatataa atataatggt
     7201 attgaaattt cggaaaggaa t
//
LOCUS       DBNFCK010000096         7132 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.096, whole genome shotgun sequence.
ACCESSION   DBNFCK010000096 DBNFCK010000000
VERSION     DBNFCK010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7132
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.096"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            130..1464
                     /gene="trmFO"
                     /locus_tag="AC1PIW_001508"
     CDS             130..1464
                     /gene="trmFO"
                     /locus_tag="AC1PIW_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HIP6606435.1"
                     /translation="MSQTHINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKPTPQHK
                     TDKFAELVCSNSFRGDSLTNAVGLLKEEMRRLDSIIMRAGEAHRVPAGGAMAMDRENF
                     SQAVTDEIHNHPLIEVIRGEITEIPEDAITVIATGPLTSDALAEKIHALNGGDGFYFY
                     DAAAPIVDSSTINMDLVYLKSRYDKGEAAYLNAPMNKEQFNAFYEALISAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPEDYKGPRDGEYKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRIFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     FATKKNPNLFFAGQMTGVEGYVESAASGLVAGINAVRRFHGEEPVIFPQTTAIGALPF
                     YITHTESKHFQPMNVNFGIIKELDGPRIRDKKERYEAIAERSLKDLEEFLTV"
     gene            1541..2377
                     /locus_tag="AC1PIW_001509"
     CDS             1541..2377
                     /locus_tag="AC1PIW_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIP6606436.1"
                     /translation="MIKEFNATVRYLESVLESEIDEREFLRISGYSVPMFSRLFSILT
                     DMSLSEYVRLRKLTRAAMDIREGQAKIIDIALKYGYESSDSFAFAFKQFHQISPSQVR
                     KGLPFKVLLPLQLSLTIQGGKEMDVRIEKKAGFTVAGLLRTAISNEVCPQVWEDLFKN
                     HTYEELAALGSGQSFGVCTLAQADETINYMAAYDVTDSEKATKLGLELLDVHEADYAI
                     FTLTGPVPQSIHAGWRYALETFFPEHGYRHSGAPDFEYYFEGDMSSPDYQMELWIPIV
                     KA"
     gene            2442..3446
                     /locus_tag="AC1PIW_001510"
     CDS             2442..3446
                     /locus_tag="AC1PIW_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase AlkZ-like family protein"
                     /protein_id="HIP6606437.1"
                     /translation="MHHNTWKGIILQKQGLLNPQSVQQICQNLNGLQAQFQPYVHVGF
                     RNRMTAEDFHEGSWQEELTRQWSIRRTVHAYLESEIPLYIHEGRLVSTDYLKTEGRDG
                     FSPQTKQKYHHLILEALEQGPMTREDLKVLCREEKMTQKEEKLVFNAWGGLFRYMVER
                     GEVYQEYGAKRFHRLTDFQPLSREKAELEIARRYFSGFGPVSLADARYYFKENKSTIL
                     EWMAQLDLKTIQVAGEERFYLGVLDEADLPQCLFVAGFDQLLLGYEKKNNPFFDPKYI
                     RNIYTLTGIVKPVVFYKGRFVATWKRDKGTILLDIFESITQKEEKELDYYRQMYEKIL
                     "
     gene            3560..4387
                     /locus_tag="AC1PIW_001511"
     CDS             3560..4387
                     /locus_tag="AC1PIW_001511"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIP6606438.1"
                     /translation="MNKSYFYLDMRTHELDVPFTERKRRVRVLLPKGYAENTEETYPV
                     VYFHDGQNVLYSKEAFSGHSWKVIPTIKRNPDIAKMIVVAIDNDGADRMHEYAAWKFS
                     EMGIPGVQFGGKGTLYAEFVMDVVKPFIDQEYRTKSDKVHTAMIGSSLGGNITQFMGL
                     AYQNQIGCLGVFSSANWLHQDAFDRYIERQQLDSEQRVYIYVGTEEADDTDKTLMAGN
                     IKQAYINSSLTYYRQLIAGGVDLDNIALEVVSGAVHNEEAWALYLPACLRFLSEHWS"
     gene            4418..5164
                     /locus_tag="AC1PIW_001512"
     CDS             4418..5164
                     /locus_tag="AC1PIW_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002909732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HIP6606439.1"
                     /translation="MHVEFLSHWSGNLGREMNLNRYGHVGIPVVVFASSGGSYNEYAD
                     FGMIEACRGSIEAGQVQFFTLTSYDSESWLADWKSIHDKAEAHRAYERYVIEEAIPFI
                     KHKTGWFDGMMTTGCSMGAYHALNFFLQHPDVFTKVIALSGVYDARFFNNNQDYGNDD
                     VVYQNSPSDYIWNQNDGWFIDKYRQADIIVCTGLGDWEQDGLDSFYNLKRAFEEKSIP
                     AWFDTWGTDVAHDWVWWRKQMPFFLHSIGL"
     gene            5182..6348
                     /locus_tag="AC1PIW_001513"
     CDS             5182..6348
                     /locus_tag="AC1PIW_001513"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIP6606440.1"
                     /translation="MNYLVISPFYPENFQPFTIELAKKEGVTVLGIGQEPYDQLPEEL
                     RNALTEYFRVENLENLDEVKRAAAFLIYKHGPIDRVESHNEYWLENDAALREQFNIFG
                     AKPKHLKKTKFKSEMKKYFKKAGVPVVPGVVVKSERDFGRALKNVGFPMIAKPDNGVG
                     ASGAFKIENKEDFDRFVETWDGATIYFLEKFVNSSIITTYDGLLDYEGNVVFETGLTY
                     VHTPFELMQTKKDNAYFIEKELDFKLVEYGRAIIKAFGMRERFFHIEFFKDGDDYIAI
                     EYNNRMAGGFTVEAYNYAHSIDLFRDYANVATGGTVEERRFEPQYCLVATRRDTTEYV
                     HSADDIHQTFAGKIKTVKRMPDAFAELQGNDAYLLVTESKKELDEMIAYIGLTK"
     gene            6488..6628
                     /locus_tag="AC1PIW_001514"
     CDS             6488..6628
                     /locus_tag="AC1PIW_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606441.1"
                     /translation="MSFKFLQAKRIEEKVFSEKEKAELMEQALFLEELAHNLQSMRFM
                     AY"
     gene            6641..7018
                     /locus_tag="AC1PIW_001515"
     CDS             6641..7018
                     /locus_tag="AC1PIW_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NisI/SpaI family lantibiotic immunity
                     lipoprotein"
                     /protein_id="HIP6606442.1"
                     /translation="MKKKVISMLALGSIILTACSSSSAGFSFDEGYQTFHYKGKVYAV
                     SKQEVAEDSIQGTEVQFMEWPVVKEEGAVKKTVSLNNLYVTTNKEWAIGVQDGYYKVD
                     LEENVAESDRIDYQSVLENVDFT"
BASE COUNT         2106 a         1355 c         1642 g         2029 t
ORIGIN      
        1 ttcaatgaag gtagttttgt cctttctagc cattttctgg tatactagat aggtataaaa
       61 tttagaaagg ttctgttttt gggtattccc aaacagtagt cagatattct ggctagaggt
      121 attagtttca tgtctcaaac ccacattaat gttataggag ctggcttggc tggctcagaa
      181 gctgcttatc agattgccaa acgtggcatt cctgtcaaac tctacgagat gcgtggtgtc
      241 aagccgactc ctcagcacaa gacggacaag tttgcggagt tggtttgttc caattcattc
      301 cgtggtgata gtttgaccaa cgctgtcggc cttcttaagg aagagatgcg tcgtttggat
      361 tccattatca tgcgtgctgg agaggctcac cgtgttcctg ctggaggtgc catggccatg
      421 gatcgtgaga atttctctca agcggttact gatgagattc acaatcaccc gctgattgag
      481 gtcatccgtg gtgagattac cgaaattcct gaagatgcta ttacagtcat tgcgacgggg
      541 cctttgacgt ctgacgcctt ggcggagaag attcacgcgc ttaatggtgg tgacggtttt
      601 tacttctacg atgcggcagc accgattgtc gacagctcga ccattaacat ggacttggtt
      661 tatcttaagt ctcgttacga caagggagag gctgcctatc tcaatgcccc tatgaacaag
      721 gagcagttca acgctttcta tgaggccttg atttcagccg aggaagcacc gcttaactcc
      781 tttgaaaaag aaaaatactt tgaaggctgt atgcccattg aagtcatggc caaacgtggt
      841 atcaaaacca tgctctatgg cccaatgaag ccagttggtc tggaatatcc agaagattac
      901 aagggaccac gtgatggcga atacaagaca ccttacgcag ttgtccagct ccgtcaggat
      961 aacgcagcag gtagccttta caatattgtt ggcttccaga ctcaccttaa gtggggcgag
     1021 cagaagcgga tcttccagat gattccagga ctcgaaaatg ctgagtttgt ccgctacggc
     1081 gttatgcacc gcaattccta catggattcg ccaaacttat tggaacagac ctttgcgacc
     1141 aagaaaaatc caaatttgtt ctttgcaggt caaatgacag gagtagaagg ctatgtcgag
     1201 tctgccgcct ctggcttagt agccggtatc aatgctgttc gccgtttcca tggcgaagag
     1261 ccagtcatct tcccacagac cacagccatc ggagctctgc cattttacat cacccacacc
     1321 gaaagcaagc acttccagcc tatgaatgtc aactttggta tcatcaagga gctggacgga
     1381 ccgcgtatcc gcgataagaa ggagcgttat gaggcaatag cagagcgttc cctcaaggac
     1441 ttggaagagt ttttgacggt ttaagccgat agttttccga cgaaaagtat cgggaaaact
     1501 tggatggtag atggtaaact gatagggaaa ggggaagttg atgataaaag agttcaatgc
     1561 aaccgtacgt tacctagagt ccgttttaga aagcgaaatc gatgaacggg aatttttgcg
     1621 catatcagga tattccgttc ccatgtttag tcgtctcttt tcgattctga cagatatgtc
     1681 cttgtcagaa tacgttcggt tacgaaagtt gacgcgagct gctatggaca ttcgtgaagg
     1741 acaggcaaag attatagata tagcccttaa atatgggtat gaatcttcgg attcttttgc
     1801 ctttgccttc aaacaatttc atcagatttc tcctagtcaa gtgcgtaagg ggttgccttt
     1861 taaggtcctc ttacccttgc aactatcatt aaccatccaa ggaggtaaag aaatggatgt
     1921 tcgtattgaa aagaaggctg gttttacagt tgctggactt cttcgcacag caatcagtaa
     1981 cgaagtgtgt ccgcaggtat gggaagactt gttcaagaac catacttatg aagagttagc
     2041 tgctctagga agtgggcagt cctttggagt ttgtacatta gctcaagcgg acgagacgat
     2101 taattatatg gctgcttatg atgtcacaga tagtgaaaaa gcgactaagt taggcttaga
     2161 acttcttgat gttcacgaag ctgactatgc tatttttacc ctaacaggcc ctgttcctca
     2221 atctattcat gcaggatggc gttatgcgct agaaactttt tttcctgaac acggctatcg
     2281 ccattcaggt gcaccagatt ttgagtacta ttttgaagga gacatgagtt ccccagacta
     2341 ccaaatggaa ctctggattc ccattgtgaa agcataatgt acaggacacc ttcgggtgtc
     2401 ttttgtgtta tactgggatt agaaagcgga ggagaaatac gatgcaccac aatacttgga
     2461 agggaattat tctgcagaaa caaggtctgt tgaaccccca gtctgttcaa caaatctgcc
     2521 aaaatctcaa tggtttgcag gctcagtttc agccctatgt tcatgtcgga tttcgtaacc
     2581 gtatgacagc ggaggatttt catgagggta gctggcagga ggaactgact cgtcagtggt
     2641 ccattcggcg aacggttcat gcctatctcg agtcggaaat tcctctctat atccatgagg
     2701 ggcggctggt cagcaccgat tacctcaaga cagaaggacg ggacggtttt agtcctcaga
     2761 ccaagcagaa atatcaccat ctgattttag aagcccttga acaaggtcct atgacccgtg
     2821 aggatctcaa ggtcctttgc cgagaggaaa agatgacaca gaaagaggag aagctggtct
     2881 ttaatgcctg gggcggtctc tttcgctaca tggtcgagcg tggggaagtc taccaagagt
     2941 acggtgccaa gcgttttcat cgtctgacag actttcagcc actttctcgg gaaaaagctg
     3001 agctggaaat agcacgtcgg tatttttcag gctttgggcc agtcagtcta gcagatgctc
     3061 gttattattt caaagaaaat aagtcaacca ttctagagtg gatggctcaa ttagatttaa
     3121 aaacaattca agtagctgga gaggagcggt tttaccttgg ggtgcttgat gaggcagatt
     3181 tacctcagtg tcttttcgtt gctgggtttg atcaattact tttgggctat gaaaagaaaa
     3241 acaacccatt tttcgatcca aaatacatcc gtaacattta tacgttgacg ggtattgtca
     3301 aacctgttgt attttataaa gggcgatttg tggcaacctg gaaacgtgat aaaggtacca
     3361 ttttgctaga tatttttgaa agcatcaccc aaaaagaaga aaaagaatta gattattacc
     3421 gccaaatgta tgagaaaatt ctgtgacatt gactaactat gttcggaaaa tccgaaaatt
     3481 gcaaataaat aactaaattt tctgaatatt ccagaaaaac tatgatataa tacttaaaat
     3541 acgtaaggaa gagatagata tgaataagtc ttacttttat ttggacatga gaactcatga
     3601 attagatgta ccatttacag aaagaaaacg tcgagtccgt gttttattgc cgaaaggata
     3661 tgctgaaaat acagaagaga cctatcccgt tgtttatttc cacgatgggc aaaatgtact
     3721 ctacagtaag gaagcttttt caggtcattc atggaaagtc atcccaacta ttaagcgtaa
     3781 tccagacatt gcaaaaatga tcgtagtggc tattgataac gatggtgctg atcgcatgca
     3841 tgaatacgct gcttggaagt ttagtgagat ggggattcct ggggtgcagt tcggtggtaa
     3901 gggaaccttg tatgctgaat ttgtgatgga tgtcgtcaaa ccatttattg atcaagaata
     3961 ccggacaaaa tctgataaag ttcacactgc aatgattggc tcatccttgg gaggcaatat
     4021 cacccagttt atgggattgg cttatcagaa ccagattggc tgtctaggag tattttcatc
     4081 tgccaactgg cttcatcaag atgctttcga ccgttatatt gaacgtcaac aattagactc
     4141 agagcaacga gtctacatct acgttgggac agaagaagca gatgatacag ataaaacatt
     4201 gatggcagga aatatcaagc aggcctatat caattcttca ttgacttact atcgtcaatt
     4261 gatcgctggc ggagtggatt tggacaatat cgccttggaa gtcgtttcag gtgcggtcca
     4321 taatgaagag gcttgggcgc tctatttgcc agcttgccta cgattcctaa gcgaacattg
     4381 gtcataaaac aaaaatatac aaataggagg ctttactatg cacgttgaat ttttaagtca
     4441 ttggtctgga aatcttggcc gagaaatgaa tctaaatcgt tatggtcacg tagggattcc
     4501 tgttgtcgta tttgcttcat caggtggttc ttacaatgaa tatgctgatt ttggtatgat
     4561 tgaagcttgt cgtggctcta ttgaagctgg tcaggtccaa ttttttactc taactagcta
     4621 cgatagtgaa agctggttgg cagactggaa atccatccat gacaaggcag aggctcaccg
     4681 tgcctatgag cgttatgtga ttgaagaagc gattcccttt atcaaacata aaactggttg
     4741 gtttgatggg atgatgacaa ctggttgttc tatgggagct tatcacgcgc tcaatttctt
     4801 cctgcaacat ccagatgttt tcacgaaagt tatcgccctt tcaggtgtct atgatgctcg
     4861 tttcttcaac aacaatcaag attatggcaa tgatgatgtt gtttatcaga actcaccatc
     4921 agattacatt tggaaccaaa acgacggctg gtttattgat aaataccgtc aggctgatat
     4981 tatcgtctgt acaggtttgg gcgattggga gcaagacggt ttggactcat tctataacct
     5041 caaacgtgca tttgaagaga agagtatccc agcttggttt gatacttggg gaacagatgt
     5101 ggcccacgac tgggtgtggt ggagaaagca aatgccattc ttccttcatt ctatcggact
     5161 ataagctaga aaggactacc tatgaattat ctcgttattt caccttttta tccagaaaat
     5221 ttccaaccat ttaccattga attggcaaaa aaagaaggcg ttacggttct aggtatcggg
     5281 caagaacctt acgatcaatt gcctgaagaa cttcgtaatg ccttaacaga atacttccgt
     5341 gttgagaatc ttgaaaactt ggatgaagtc aagcgtgcag cagcctttct tatttacaag
     5401 catggtccaa ttgaccgtgt agaatcccac aatgaatatt ggcttgaaaa cgatgcagcc
     5461 ctccgtgaac aattcaacat ttttggtgct aagcctaaac acctgaaaaa gaccaaattt
     5521 aagtctgaaa tgaaaaaata tttcaaaaaa gcaggtgttc cagttgttcc aggtgtggtt
     5581 gttaaatcgg aacgtgattt tggcagagct ttgaagaatg ttggttttcc aatgattgct
     5641 aaaccagaca acggtgttgg agcatcaggt gccttcaaaa tcgaaaataa agaagatttt
     5701 gatcggtttg tggagacatg ggatggagca actatttatt tcctcgaaaa atttgtcaat
     5761 tcaagtatta tcactactta tgatggtttg ttggactacg aaggcaatgt tgtctttgaa
     5821 acaggcttga cttatgttca tactccattt gaattgatgc aaaccaagaa ggacaatgcc
     5881 tactttatcg aaaaagaatt agattttaaa ctggttgagt atggcagagc tattatcaag
     5941 gcctttggta tgagagagcg ttttttccat attgaatttt tcaaggatgg ggacgactac
     6001 attgccattg aatacaataa ccgtatggca ggcggtttca cagttgaagc ctataactac
     6061 gctcattcca ttgatctttt ccgtgattat gccaatgtcg caacaggtgg tacagttgag
     6121 gaacgtagat ttgaaccaca atactgcttg gtagcaaccc gccgtgatac gaccgaatat
     6181 gttcactcag cagatgatat ccatcagaca ttcgctggta agattaagac agtcaaacgg
     6241 atgccagatg cctttgcgga acttcaggga aatgatgcct atcttcttgt aacggaaagc
     6301 aagaaagagt tagatgaaat gattgcttat attggtctaa caaaataaaa ctaactgtaa
     6361 gacgccagaa atggtgtctt ttattctttc cttacaaagc tgtaagtttt ttaatcagaa
     6421 ctgtaagaga gtacaggagg aatattttct ataatagact cataagaaat agaaagaagg
     6481 aaatcatatg tcattcaaat ttctacaagc aaagagaata gaagaaaagg tattctctga
     6541 aaaagagaaa gctgaactca tggaacaagc cttattctta gaagagctcg cccataattt
     6601 gcaaagtatg cgatttatgg catactagag ggaggaaact atgaaaaaga aagtaattag
     6661 catgctggct ttgggaagca taatcctaac tgcttgctct tcctcttcag caggttttag
     6721 ctttgatgag ggctatcaaa ctttccatta caagggaaaa gtctatgccg ttagcaaaca
     6781 ggaagttgcc gaagatagta ttcaaggaac agaagttcag tttatggaat ggcctgttgt
     6841 caaagaagaa ggggcagtga aaaagacagt atccttgaat aatctctatg taaccactaa
     6901 caaggaatgg gccatcggtg tccaggatgg ttactataag gtagatttag aagaaaatgt
     6961 tgctgaatca gaccgaatcg actatcaaag tgtacttgag aatgttgatt ttacttaaga
     7021 aatcaattag gagtttatat gttattagaa atcaatcatt taaaaaaagt ttacaaagac
     7081 gttaccactg ttgatattga aaagattact gtacgagaag gagaaatcta cg
//
LOCUS       DBNFCK010000097         7123 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.097, whole genome shotgun sequence.
ACCESSION   DBNFCK010000097 DBNFCK010000000
VERSION     DBNFCK010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7123
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.097"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            27..874
                     /locus_tag="AC1PIW_001516"
     CDS             join(27..330,330..874)
                     /locus_tag="AC1PIW_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HIP6606443.1"
                     /translation="MAYSLDFRKKVLAYCEKTGSITEASAIFQVSRNTIYQWLKLKEK
                     TGELHHQVKGTKPRKVDRDKLKNYLETHPDAYLTEIASEFDCHPTAIHYPLKAMGYTP
                     KKKSCTYYEQDPEKVELFLKELNNLSHLTPVYIDETGFETYFHRKYGRSLKGQLIKGK
                     VSGRRYQRISLVAGLINGALIAPMTYKDTMTSGFFEAWFKTFLLPTLGKPSVIIMDNA
                     KFHRMSKLKDLCEEQGHRLLPLPPYSPEYNPIEKIWAHIKKHLRRVLPNCDTFLEALS
                     SCSCFS"
     gene            complement(875..1156)
                     /locus_tag="AC1PIW_001517"
     CDS             complement(875..1156)
                     /locus_tag="AC1PIW_001517"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6606444.1"
                     /translation="MIVPLQFEHILQLDEAFQKQGWPSRKEILTNYLREQEKGERIVL
                     VAEETGVCKGYITLIKQVKEGQFYQSGIPEIADFNVFEDFQNQGIGWQL"
     gene            complement(1158..2411)
                     /locus_tag="AC1PIW_001518"
     CDS             complement(1158..2411)
                     /locus_tag="AC1PIW_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HIP6606445.1"
                     /translation="MHQITCPHCGTAFQVNETEYSQLLAQVRGAEFDKEIYDRLERER
                     ELLSQKAENDLQARLSDKDKEILELTAKLDKFASQTELEISSAISQKDQEIQELKAQL
                     GQIGLAKDLELQQAVAQVEKERDAAQNALVLQEQKQELALATTRQEYEVRLKAADEQV
                     EFYKNFKAQQSTKAIGESLEQFAEAEFNKVRSYAFPRAKFAKDNEVSASGSKGDFIFR
                     DFDESGLEFISIMFEMKNEADTTKTKHKNADFFKELDKDRREKKCEYAVLVSMLEADN
                     DYYNIGIVDVSHEYEKMYVIRPQFFIQLIGILRNAALNSLQYQQELALVKEQNIDITH
                     FEEDLEIFKNAFAKNYQSASNNFQKAIDEIDKAIKRMEAVKAALTTSENQLRLANNKL
                     DDVSVKKLTRNNPTMKAKFEALRED"
     gene            complement(2418..2675)
                     /locus_tag="AC1PIW_001519"
     CDS             complement(2418..2675)
                     /locus_tag="AC1PIW_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606446.1"
                     /translation="MKKWWEQLKEEYYETRFDSNEDWMEWFIKRKMTWGWRWPTAITI
                     FVLWALSLPDARNAVRFFYFLLALGVATVLHKLISYLKRRK"
     gene            complement(2693..3163)
                     /locus_tag="AC1PIW_001520"
     CDS             complement(2693..3163)
                     /locus_tag="AC1PIW_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HIP6606447.1"
                     /translation="MKISVKKEAGHYLFSICYSVSASLAESWDLLATDSGFAHWFPQL
                     NIVGNTLVFEMEGFREVMDLLEYRKNEKIAYRWDSATVSFALSQLENQTLITFEERIP
                     EDFGNEFANAQKDMTGWLVQNECIKKFLEGQEPPVCQPLQEKWRTFLELELEGL"
     gene            3545..4360
                     /locus_tag="AC1PIW_001521"
     CDS             3545..4360
                     /locus_tag="AC1PIW_001521"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIP6606448.1"
                     /translation="MIKLIALDMDGTLLNEKKELMQPQIDAIHQAVEAGFKIVLCTGR
                     PLAGVKPFVEQLGFDTEEEFIIVNNGCSTHSTKDWSLIDWEELSISDIDYLSTFIENA
                     DVQISLFDEEDYFVLAEKANARVNLDAGLVGMTPQPIDLKEAQSGQYRFFEAMFVGEK
                     VHIDAFENQHNPVLSKRYSTVRSQDYLLEILPNGASKASGLKKLADRLGILPEEIMAM
                     GDANNDLEMIEFAGLGIAMGNANEQVKAIAQDITDTNENNGVAKAIEKHILNK"
     gene            4370..5590
                     /gene="pepT"
                     /locus_tag="AC1PIW_001522"
     CDS             4370..5590
                     /gene="pepT"
                     /locus_tag="AC1PIW_001522"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HIP6606449.1"
                     /translation="MKYPTLLDRFLVYVKENTRSDENSTTTPSTQNQVEFAQNILLPE
                     MERIGLQNVHYLPNGFAVGTLPANDPSLTRKIGFIAHMDTADFNAEGVNPQIIENYDG
                     NPIALGTSGYELHPKDFPQLANYHGQILITTDGTTLLGSDDKSGIAEIMTAIEFLIQN
                     PDIKHCEIRVGFGPDEEIGVGADKFDVKDFDVDFAYTMDGGPLGELQYETFSAAGAKI
                     DFLGRNVHPGSAKDQMINAFQMAIDFHNALPETDRPEKTEGYEGFFHLMNMEGSVDTA
                     STTYIIRDFEEEDFQARKQLMLDIAEKMNANFDTPRVIVNLHDQYYNMKKIIEKDMTP
                     INIAKDVMENLGIKPLIEPVRGGTDGSKISFMGIPTPNIFAGGENMHGRFEFVSLETM
                     EKAVDVILGIVAYK"
     gene            5718..6116
                     /locus_tag="AC1PIW_001523"
     CDS             5718..6116
                     /locus_tag="AC1PIW_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HIP6606450.1"
                     /translation="MTEETLAQGILIGIWGTTLLFSFIWYILVAISNYILFKKAGYAG
                     WKSLIPIYNLYIQQCITFGYEKRWFILFLLIPLAGPLYGIYLVYNFGRSFGLSAVQAI
                     FYVLLTPIFNLYIAFNDGSRYQGPQEFFID"
     gene            complement(6152..7066)
                     /locus_tag="AC1PIW_001524"
     CDS             complement(6152..7066)
                     /locus_tag="AC1PIW_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIP6606451.1"
                     /translation="MNELELLARALGFIFVIGLGYTLKVKGIVRREDARIFSSLVMNV
                     TLPASLLVASATTQVSIDLYLPFILGILCNLFLDMVGYWEAKKSGQQTSVGLMQLSGY
                     NIGTFTLPFVQAFFPLSSLLSVIVFDAGNAIMVLGGNYSIAAGVDEEQERMTVATFIK
                     NLSRSVPLVVYLVSLSLAVCSIKLPRELISALTIASNANPFLAMLMLGILLDLKLNWQ
                     EISRLAYLLSLRLGANILIAGVIYFLLPIDKTMKIMLLVCLVSPISVMSPIYALKLGS
                     RSAEPANVNSLSILVSLVMMVLLIFLFV"
BASE COUNT         2158 a         1603 c         1295 g         2067 t
ORIGIN      
        1 cagtacattt atgatataat gtatttatgg catattcatt agattttcgt aaaaaagttc
       61 tcgcatactg tgagaaaacc ggcagtatta ctgaagcatc agctattttc caagtttcac
      121 gtaacactat ctatcaatgg ctaaaattaa aagagaaaac cggcgagctt catcaccaag
      181 ttaaaggaac caagccaaga aaagtggata gagataaatt aaagaattat cttgaaactc
      241 atccagatgc ttatttgact gaaatagctt ctgaatttga ctgtcatcca acagctattc
      301 attaccccct caaagctatg ggatatactc gaaaaaaaag agctgtacct actatgaaca
      361 agaccctgaa aaagtagaac tgttccttaa agaattgaat aacttaagcc acttgactcc
      421 tgtttatatt gacgagacag ggtttgagac atattttcat cgaaaatatg gtcgctcttt
      481 gaaaggtcag ttgataaaag gtaaggtctc tggaagaaga taccagcgga tatctttagt
      541 agcaggtctc ataaatggtg cgcttatagc cccgatgaca tacaaagata ctatgacgag
      601 tggctttttc gaagcttggt tcaaaacatt cttactaccc actttaggta aaccatctgt
      661 tatcattatg gacaatgcaa agtttcatag gatgagtaag ctaaaagatt tatgcgagga
      721 gcagggacat agacttttac cacttcctcc ttactcaccg gaatataatc ccattgagaa
      781 aatatgggct cacatcaaaa aacacctcag aagagtattg ccaaattgcg atacttttct
      841 tgaggcactt tcgtcctgct cttgtttcag ttgactataa ttgccaacct atcccctgat
      901 tttgaaagtc ttcaaaaaca ttgaaatcag caatttcagg tattccagat tggtaaaact
      961 gtccctcctt cacttgctta atcaaggtga tataaccttt acaaaccccc gtttcttcag
     1021 caactaggac gatacgttct cccttttctt gttcccttaa atagttggtt aaaatctctt
     1081 tgcgggaggg ccaaccttgc ttctggaagg cctcatcaag ttgaagtata tgctcaaatt
     1141 gtaacggaac aatcatctca atcctccctc aacgcctcaa acttggcttt catggttggg
     1201 ttattgcggg ttagtttttt gacggagaca tcgtctagtt tattattagc taggcgaagc
     1261 tggttttcgc tggtggtcag ggctgctttg acagcctcca ttcgcttgat tgccttgtcg
     1321 atttcatcaa tagctttttg gaaattgttg ctggccgatt gatagttttt ggcaaaagca
     1381 ttcttgaaaa tttccaaatc ttcttcaaaa tgagtaatat caatattctg ctccttaacc
     1441 aaggctaact cctgctgata ttgcagacta ttgagcgcag catttcgtaa aattccgatt
     1501 aattggataa agaactgtgg tcgaataaca tacatcttct catattcatg actaacatcc
     1561 acaatcccta tattgtaata gtcattgtcc gcctctagca tgctgactaa tacagcatat
     1621 tcgcattttt tctcccgacg gtccttatcc aactccttaa agaaatcagc gtttttatgc
     1681 ttggtcttgg tcgtatctgc ctcgtttttc atttcgaaca tgatggaaat aaattccaag
     1741 cccgactcgt caaaatcacg gaagatgaaa tctccttttg aaccggacgc tgatacttcg
     1801 ttgtccttag caaatttggc acgtggaaaa gcataggaac gaaccttgtt aaactccgcc
     1861 tcagcaaatt gctccaagct ctctccaatt gccttagtag actgctgagc cttgaaattt
     1921 ttgtaaaatt ccacctgctc atccgccgcc ttgagccgta cttcgtactc ctgacgagtg
     1981 gtcgccaggg ctaactcttg cttttgttcc tgcaaaacca aagcattttg tgccgcatcc
     2041 ctctcttttt ccacctgagc taccgcctgc tgcaactcca agtccttggc gagaccgatt
     2101 tgccccaact gagccttaag ctcttggatt tcttggtctt tttgggaaat agcagagctg
     2161 atttccagtt ccgtctgact agcaaattta tcaagttttg cggtcagttc cagaatctcc
     2221 ttatccttat cactcaatct agcctgcaaa tcattttcag ccttctggct taataattcc
     2281 cgttcccgct ccaaacggtc gtagatttcc ttatcaaact ccgcaccacg cacttgggct
     2341 aaaagctgac tatattctgt ttcattgacc tgaaaggccg tcccacaatg ggggcaagta
     2401 atttgatgca tatactctca ctttcttctt ttcaaataag atattaattt atgtaaaacc
     2461 gtagctactc ccagcgccaa taaaaaataa aagaaacgaa ccgcatttct agcatctgga
     2521 agtgatagag cccaaaggac aaaaatcgtt atggcagtcg gccatctcca gccccaggtc
     2581 atcttccgtt tgataaacca ttccatccaa tcttcattag aatcaaaccg tgtttcataa
     2641 tattcttctt tcagttgttc ccaccacttt ttcataacag cctcctttca gatcacagac
     2701 cttctaattc aagctccaga aaagtcctcc acttttcctg caagggttga cacacaggcg
     2761 gctcctgacc ttctaagaat tttttgatac attcattttg caccaaccag cctgtcatgt
     2821 ctttttgagc attcgcaaat tcattaccaa aatcttctgg aattcgttct tcaaaagtaa
     2881 tcaaggtctg attttctagc tgagacagtg caaatgaaac tgttgctgaa tcccacctgt
     2941 aggcaatttt ctcatttttt cggtattcca gtaaatccat aacttcacga aatccttcca
     3001 tttcaaaaac cagcgtattt cctacaatgt tcaactgtgg aaaccagtgg gcaaaaccac
     3061 tgtctgtcgc caacaaatcc caggactccg ccaaactagc cgacacagaa tagcaaatgc
     3121 tgaacaggta gtgccccgcc tctttcttca cactaatctt catatcatgc tcctattcta
     3181 tccttctatt aattatacca aaaaacaccg gtctataacc gatatttcca ttgtctctat
     3241 tctttaagca tgaatgctat tcattccaga ggatcgaaaa acggtatctt gaaatctgaa
     3301 ttatttttta taacatcttt catagactgt ccttaccttt gtttatctgg ctcatactcc
     3361 atgttttgta tcccctagca atgttagact ttcctgccac ataatggaca ttgtcatttt
     3421 tgaaacactt ctatatttca ttcgtcacct aatttatttt aacatataga acagagttta
     3481 gtatttcaat cattcctttc tcattttatg ctatactaga tgtattgaaa acaaggaggt
     3541 ttatatgata aaactaatcg ctcttgatat ggacggaacc cttttaaacg agaaaaaaga
     3601 gctgatgcag ccacagattg atgccattca tcaggctgtt gaagcgggct tcaagattgt
     3661 tctttgtact ggtcggccct tggctggtgt taaacctttc gtagagcagc tcggctttga
     3721 caccgaggaa gaatttatca ttgtcaataa cggctgttca acccattcga caaaggattg
     3781 gagcctgatt gactgggaag aactatctat ctctgatatt gattatcttt ccacttttat
     3841 tgaaaatgct gatgtgcaaa tctctctttt tgatgaggaa gactacttcg ttcttgcaga
     3901 aaaagccaat gcacgcgtta atttagacgc aggtttggta ggaatgactc ctcaaccaat
     3961 tgatttaaaa gaagcccagt caggtcagta tcgcttcttt gaagccatgt ttgtcggtga
     4021 aaaagtgcat attgatgcct ttgaaaatca acacaatcct gtactcagca aacgctattc
     4081 caccgttcgt tcacaggact atctcttgga aattttacca aacggtgcca gcaaggcctc
     4141 tggtttaaaa aaattggcag accgcctcgg tatcctccca gaagaaatca tggctatggg
     4201 agatgccaac aacgacctgg aaatgattga atttgctgga cttggtattg ccatgggaaa
     4261 tgccaacgag caggtcaagg ctatcgccca ggacatcaca gacaccaatg aaaacaacgg
     4321 cgttgccaag gccattgaaa aacacatttt gaacaaataa ggagacaata tgaaataccc
     4381 aacactctta gaccgtttct tggtctatgt caaagaaaat actcgctcag atgaaaattc
     4441 gacaaccact ccttctaccc aaaaccaagt cgagtttgca cagaatatcc ttctgcctga
     4501 aatggaacgg attggtttgc agaatgtcca ctacctacca aacggttttg cagtgggaac
     4561 actgcctgcc aacgacccaa gtttgacccg caagattggc ttcatcgccc acatggatac
     4621 ggctgatttc aacgcagaag gtgtgaatcc tcagattatc gaaaactacg acggtaaccc
     4681 aattgccttg ggaacttctg gctatgaatt gcatccaaaa gacttcccac aacttgccaa
     4741 ctaccacggt caaatcctga ttaccacaga cggtactacc ttgctaggct ctgatgacaa
     4801 atcaggaatt gcagaaatca tgacagctat tgaattccta atccaaaacc ccgacatcaa
     4861 acactgcgaa atccgtgtcg gctttggtcc tgacgaagaa atcggtgtcg gagcagacaa
     4921 gtttgatgtg aaagacttcg atgtggactt tgcctacact atggacggtg gaccgctggg
     4981 cgaactccag tacgaaacct tctcagcagc aggtgcaaag attgatttcc taggacgcaa
     5041 tgtccaccca ggatctgcta aggaccagat gatcaatgcc ttccaaatgg ctattgattt
     5101 ccacaatgcc cttccagaaa cagaccgccc tgaaaaaacc gaaggatatg aaggtttctt
     5161 ccacttgatg aacatggagg gcagcgttga tactgcttct accacctata tcatccgcga
     5221 ctttgaggaa gaagatttcc aagcccgcaa gcaactcatg ctggacatcg ctgaaaagat
     5281 gaatgccaac tttgacactc cacgcgtcat tgtcaaccta catgaccagt actacaacat
     5341 gaagaaaatc atcgaaaaag atatgactcc aattaacatt gctaaggacg ttatggaaaa
     5401 tctaggcatc aagccactga ttgagcctgt tcgcggagga acagacggct ctaagatttc
     5461 cttcatgggc attccaacac caaatatctt cgcaggtggc gaaaacatgc acggtcgctt
     5521 cgagttcgtc agcttagaaa ccatggaaaa agccgtcgat gtcatccttg gaatcgtcgc
     5581 ttacaaataa aatttcaaac ggctttcaat agaaagtcgt ttttttctga aaaaatttta
     5641 aaaaaatgcc attttatgtt gtgttattga actaatacta gtataataga actataaaaa
     5701 agaaatggag ttttattatg acagaagaaa cattagcaca gggaattctc attggcattt
     5761 ggggtacaac actactattt tcatttattt ggtatattct tgttgctatt tccaactaca
     5821 tcctcttcaa aaaagcaggc tacgctggtt ggaaatcatt gattccaatc tacaatctct
     5881 atatccaaca atgcatcacc ttcggctatg aaaaaagatg gttcattcta ttcttactaa
     5941 ttccactagc aggaccatta tatggcatct atctcgttta caattttggt cgtagcttcg
     6001 gtttatcagc tgtacaagct atcttctatg ttcttcttac acctatcttt aacctctaca
     6061 tcgcctttaa tgatggaagt cgttaccaag gtccacaaga attctttatt gattaatata
     6121 caatcaagcc cccgcacacg cggaggcttt tttaaacgaa tagaaaaata agaagaacca
     6181 tcatgactag gctgaccaaa atagataagg agttaacgtt ggcaggttca gctgagcgac
     6241 tacctaactt taatgcataa attggggaca tgactgaaat cggagaaact agacagacca
     6301 gcaacatgat tttcatcgtt ttatcaatag gtagaagaaa ataaatgact cctgctatta
     6361 gaatattcgc acccagcctc aaagagagaa gataggccaa tcgactaatt tcttgccaat
     6421 tcaattttaa atcaagcaag attcccaaca tgagcatggc taaaaaagga ttggcgttac
     6481 tggctatcgt cagcgcagag ataagttcgc gtggaagttt gatagagcag actgctaacg
     6541 ataggctaac tagatagact actagtggaa ctgagcgtga aagattcttt ataaaagttg
     6601 caacggtcat tctttcctgc tcttcatcca caccagctgc gatactgtaa ttcccaccta
     6661 gaaccatgat tgcattcccc gcatcaaata cgatgacact tagtagacta gatagcggaa
     6721 aaaaagcttg cacaaatggg agtgtaaagg ttccaatatt atagcctgat aactgcataa
     6781 gaccgacgga agtctgttga ccagacttct ttgcttccca gtatcctacc atatccaaaa
     6841 aaagattaca aagaatccct aatatgaagg gaaggtataa atctatagat acttgggttg
     6901 tcgctgaagc tacaaggaga gaagctggta gcgtaacatt catcactaag ctagaaaaga
     6961 ttcttgcatc ttcccttcga acaatccctt tgacttttaa tgtataacct aaaccgataa
     7021 caaagataaa accgagagca cgggctaata attcaagttc attcatgaaa ctagtatagc
     7081 atatagaaaa gaattttgat gatatataaa aattttatat aat
//
LOCUS       DBNFCK010000098         7027 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.098, whole genome shotgun sequence.
ACCESSION   DBNFCK010000098 DBNFCK010000000
VERSION     DBNFCK010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7027
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.098"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            186..1734
                     /locus_tag="AC1PIW_001525"
     rRNA            186..1734
                     /locus_tag="AC1PIW_001525"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1788..1860
                     /locus_tag="AC1PIW_001526"
     tRNA            1788..1860
                     /locus_tag="AC1PIW_001526"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1821..1823,aa:Ala,seq:tgc)
     gene            2140..5043
                     /locus_tag="AC1PIW_001527"
     rRNA            2140..5043
                     /locus_tag="AC1PIW_001527"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            5130..5245
                     /gene="rrf"
                     /locus_tag="AC1PIW_001528"
     rRNA            5130..5245
                     /gene="rrf"
                     /locus_tag="AC1PIW_001528"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            5251..5323
                     /locus_tag="AC1PIW_001529"
     tRNA            5251..5323
                     /locus_tag="AC1PIW_001529"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5284..5286,aa:Val,seq:tac)
     gene            5329..5399
                     /locus_tag="AC1PIW_001530"
     tRNA            5329..5399
                     /locus_tag="AC1PIW_001530"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5361..5363,aa:Gly,seq:tcc)
     gene            5430..5503
                     /locus_tag="AC1PIW_001531"
     tRNA            5430..5503
                     /locus_tag="AC1PIW_001531"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5464..5466,aa:Ile,seq:gat)
     gene            5510..5581
                     /locus_tag="AC1PIW_001532"
     tRNA            5510..5581
                     /locus_tag="AC1PIW_001532"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5543..5545,aa:Glu,seq:ttc)
     gene            5590..5679
                     /locus_tag="AC1PIW_001533"
     tRNA            5590..5679
                     /locus_tag="AC1PIW_001533"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5626..5628,aa:Ser,seq:tga)
     gene            5689..5762
                     /locus_tag="AC1PIW_001534"
     tRNA            5689..5762
                     /locus_tag="AC1PIW_001534"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5723..5725,aa:Met,seq:cat)
     gene            5778..5850
                     /locus_tag="AC1PIW_001535"
     tRNA            5778..5850
                     /locus_tag="AC1PIW_001535"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5811..5813,aa:Phe,seq:gaa)
     gene            5871..5951
                     /locus_tag="AC1PIW_001536"
     tRNA            5871..5951
                     /locus_tag="AC1PIW_001536"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5905..5907,aa:Tyr,seq:gta)
     gene            5959..6029
                     /locus_tag="AC1PIW_001537"
     tRNA            5959..6029
                     /locus_tag="AC1PIW_001537"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5991..5993,aa:Trp,seq:cca)
     gene            6037..6109
                     /locus_tag="AC1PIW_001538"
     tRNA            6037..6109
                     /locus_tag="AC1PIW_001538"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6070..6072,aa:His,seq:gtg)
     gene            6121..6192
                     /locus_tag="AC1PIW_001539"
     tRNA            6121..6192
                     /locus_tag="AC1PIW_001539"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6153..6155,aa:Gln,seq:ttg)
     gene            6204..6287
                     /locus_tag="AC1PIW_001540"
     tRNA            6204..6287
                     /locus_tag="AC1PIW_001540"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6238..6240,aa:Leu,seq:caa)
     gene            complement(6374..>7027)
                     /locus_tag="AC1PIW_001541"
     CDS             complement(6374..>7027)
                     /locus_tag="AC1PIW_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HIP6606452.1"
                     /translation="PVENIRKLPISKPTINFWNVEEFNEFRKLITSDEISYDLFFTIA
                     FFTGMRLGEIVALTWYDINLITNSIHITKTAYFVNGTNHINSTKTRAGTRRITINHKL
                     ARMLADWKEKQKDLLKEFTTDTENLQVIQSTPLPMTKNMIDKKFKQILARNDYLKKIR
                     IHDLRHSHASLLINQGEDYLVVKERLGHASITTTIDTYSHLYPSKQKTLANKLDDIF"
BASE COUNT         1908 a         1433 c         1922 g         1764 t
ORIGIN      
        1 tgacaaagtt cacaagaaat gataaactaa gatagttgtc gcgagagcgc gataacgaca
       61 agacctttga aaattaaaga agacgaacca aacgtgcagg gtgatttatc taaagataaa
      121 tcgtcaatga caaaacaaaa caataaaacg gaaagctagc aatagcttga gtttgaatca
      181 aaacttttta tgagagtttg atcctggctc aggacgaacg ctggcggcgt gcctaataca
      241 tgcaagtaga acgctgaagt ctggtgcttg cactagacgg atgagttgcg aacgggtgag
      301 taacgcgtag gtaacctgcc tcatagcggg ggataactat tggaaacgat agctaatacc
      361 gcataacagt atttaccgca tggtagatat ttgaaaggag caattgcttc actatgagat
      421 ggacctgcgt tgtattagct agttggtgag gtaacggctc accaaggctt cgatacatag
      481 ccgacctgag agggtgatcg gccacactgg gactgagaca cggcccagac tcctacggga
      541 ggcagcagta gggaatcttc ggcaatgggg gcaaccctga ccgagcaacg ccgcgtgagt
      601 gaagaaggtt ttcggatcgt aaagctctgt tgtaagagaa gaactgtgag aagagtggaa
      661 agtttctcac ttgacggtat cttaccagaa agggacggct aactacgtgc cagcagccgc
      721 ggtaatacgt aggtcccgag cgttgtccgg atttattggg cgtaaagcga gcgcaggcgg
      781 tttgataagt ctgaagtaaa aggctgtggc ttaaccatag tacgctttgg aaactgtcaa
      841 acttgagtgc agaaggggag agtggaattc catgtgtagc ggtgaaatgc gtagatatat
      901 ggaggaacac cggtggcgaa agcggctctc tggtctgtaa ctgacgctga ggctcgaaag
      961 cgtggggagc gaacaggatt agataccctg gtagtccacg ccgtaaacga tgagtgctag
     1021 gtgttgggtc ctttccggga ctcagtgccg cagctaacgc attaagcact ccgcctgggg
     1081 agtacgaccg caaggttgaa actcaaagga attgacgggg gcccgcacaa gcggtggagc
     1141 atgtggttta attcgaagca acgcgaagaa ccttaccagg tcttgacatc ccgatgaccg
     1201 ccctagagat agggtttctc ttcggagcat cggtgacagg tggtgcatgg ttgtcgtcag
     1261 ctcgtgtcgt gagatgttgg gttaagtccc gcaacgagcg caacccctat tgttagttgc
     1321 catcattcag ttgggcactc tagcgagact gccggtaata aaccggagga aggtggggat
     1381 gacgtcaaat catcatgccc cttatgacct gggctacaca cgtgctacaa tggctggtac
     1441 aacgagtcgc aagtcggtga cggcaagcta atctcttaaa gccagtctca gttcggattg
     1501 taggctgcaa ctcgcctaca tgaagtcgga atcgctagta atcgcggatc agcacgccgc
     1561 ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accacgagag tttgtaacac
     1621 ccgaagtcgg tgaggtaacc ttttaggagc cagccgccta aggtgggata gatgattggg
     1681 gtgaagtcgt aacaaggtag ccgtatcgga aggtgcggct ggatcacctc ctttctaagg
     1741 aaatggaaac ctgtacgtca gtcttcttta attttgagag gtcttgtggg gccttagctc
     1801 agctgggaga gcgcctgctt tgcacgcagg aggtcagcgg ttcgatcccg ctaggctcca
     1861 ttaacaacgg aagttgttaa gattttgtcc attgaaaatt gaatatctat caaacattcc
     1921 taaacgtatg taaaagtacg tatagaaata gtaacaagaa aataaaccga aaacgctgtg
     1981 aatatttaat gagttttcta atttttgaaa aaattaggtt aaaacaagtt acgaagacgt
     2041 taaggaaaac gtatgatttt aggaaatcat cgcagtagtc taaggactac aagaggattt
     2101 gtctaaatca ctagttttct agtctgagta cgattataag gttaagttaa taagggcgca
     2161 cggtggatgc cttggcacta gaagccgatg aaggacgtga ctaacgacga aatgccttgg
     2221 ggagctgtaa gtaagcaatg atccagggat gtccgaatgg gggaacccgg caggtaatgc
     2281 ctgtcactca ctactgttaa ggtagtgaag aggaagacgc agtgaactga aacatctaag
     2341 tagctgcagg aagagaaagc aaaagcgatt gccttagtag cggcgagcga aacggcagga
     2401 gggcaaaccg aggagtttac tcctcggggt tgtaggactg caatgtggac ttaaagagta
     2461 tagaagaact acctgggaag gtaggccaaa gagagtaata gcctcgtatt taaaatattt
     2521 tttataccta gcagtatcct gagtacggcg agacacgcga aatctcgtcg gaatccggga
     2581 ggaccatctc ccaaccctaa atactctcta gtgaccgata gtgaaccagt accgtgaggg
     2641 aaaggtgaaa agtaccccgg aaggggagtg aaatagaacc tgaaaccgtg tgcctacaac
     2701 aagttcgagc ccgttaatgg gtgagagcgt gccttttgta gaatgaaccg gcgagttacg
     2761 atatgatgcg aggttaagtt gaagagacgg agccgtaggg aaaccgagtc ttaatagggc
     2821 ggattagtat tatgtcgtag acccgaaacc atgtgaccta cccatgagca ggttgaaggt
     2881 gcggtaagac gcactggagg accgaaccag ggcacgttga aaagtgcttg gatgacttgt
     2941 gggtagcgga gaaattccaa acgaacttgg agatagctgg ttctctccga aatagcttta
     3001 gggctagcgt cgacatttga gaatcttgga ggtagagcac tgtttggatg agggggccat
     3061 ctcggtttac tgatttcaga taaactccga atgccaatga tttatggtcg gcagtcagac
     3121 tgcgagtgct aagatccgta gtcgaaaggg aaacagccca gaccaccagc taaggtccca
     3181 aaataattgt taagtggaaa aggatgtggg gttgcacaga caactaggat gttagcttag
     3241 aagcagctat tcattcaaag agtgcgtaat agctcactag tcgagtgacc ctgcgccgaa
     3301 aatgtaccgg ggctaaaaca atttaccgaa gctgtggata acactttagt gttatggtag
     3361 gagagcgttc tatgtgtgaa gaaggtatac cgtgaggagt gctggaacgc atagaagtga
     3421 gaatgccggt atgagtagcg aaagatgggt gagaatccca tccaccgtaa gactaaggtt
     3481 tccaggggaa ggctcgtccg ccctgggtta gtcgggacct aaggagagac cgaagggtgt
     3541 atccgatgga caacaggttg atattcctgt actagagtat gaagtgatgg agggacgcag
     3601 taggctaact aaagcgggcg attggaagtg cccgtctaag cagtgaggtg tgatatgagt
     3661 caaatgcttg tatctgtaac attgagctgt gatggggagc gaagttaagt agcgaagtta
     3721 gtgacgtcac actgccgaga aaagcttcta gcgatgtatc atactctacc cgtaccgcaa
     3781 accgacacag gtagtcgagg cgagtagcct caggtgagcg agagaactct cgttaaggaa
     3841 ctcggcaaaa tgaccccgta acttcgggag aaggggtgct ggcttaaagt cagccgcagt
     3901 gaataggccc aagcaactgt ttatcaaaaa cacagctctc tgctaaatcg taagatgatg
     3961 tatagggggt gacgcctgcc cggtgctgga aggttaagag gagggtttag cgcaagcgaa
     4021 gatctgaatt gaagccccag taaacggcgg ccgtaactat aacggtccta aggtagcgaa
     4081 attccttgtc gggtaagttc cgacccgcac gaaaggcgta atgatttggg cactgtctca
     4141 acgagagact cggtgaaatt ttagtacctg tgaagatgca ggttacccgc gacaggacgg
     4201 aaagacccca tggagcttta ctgcagtttg atattgagta tctgtaccac atgtacagga
     4261 taggtaggag cctacgaagt cgggacgcca gtttcgactg aggcgctgtt gggatactac
     4321 ccttgtgtta tggctactct aacccggata ggttatccct atcggagaca gtgtctgacg
     4381 ggcagtttga ctggggcggt cgcctcctaa aaggtaacgg aggcgcccaa aggttccctc
     4441 agaatggttg gaaatcattc gcagagtgta aaggtataag ggagcttgac tgcgagagct
     4501 acaactcgag cagggacgaa agtcgggctt agtgatccgg tggttccgta tggaagggcc
     4561 atcgctcaac ggataaaagc taccctgggg ataacaggct tatctccccc aagagttcac
     4621 atcgacgggg aggtttggca cctcgatgtc ggctcgtcgc atcctggggc tgtagtcggt
     4681 cccaagggtt gggctgttcg cccattaaag cggcacgcga gctgggttca gaacgtcgtg
     4741 agacagttcg gtccctatcc gtcgcgggcg taggaaattt gagaggatct gctcctagta
     4801 cgagaggacc agagtggact taccgctggt gtaccagttg tcttgccaaa ggcatcgctg
     4861 ggtagctatg tagggacggg ataaacgctg aaagcatcta agtgtgaaac ccacctcaag
     4921 atgagatttc ccataacttt atgttagtaa gagccctgag agatgatcag gtagataggt
     4981 tggaagtgga agtgtggcga cacatgtagc ggaccaatac taatcgctcg aggacttatc
     5041 caaagaaata aactagagtc aatattgaca agcgatcggt ttcttgttag aatatagata
     5101 ttcaattttg agttgacaag actcagtagt taagtgacga tagcctagga gatacacctg
     5161 tacccatgcc gaacacagca gttaagccct agaacgcctg aagtagttgg gggttgcccc
     5221 ctgttagata cggtagtcgc ttagcaaatt gggagtttag ctcagctggg agagcatctg
     5281 ccttacaagc agagggtcag cggttcgatc ccgttaactc ccatataagc gggtgtagtt
     5341 tagtggtaaa actacagcct tccaagctgt tgtcgcgagt tcgattctcg tcacccgctt
     5401 tgaacatagt tcatacccaa acttggtttg ggcgcgtagc tcagatggtt agagcgcacg
     5461 cctgataagc gtgaggtcgg tggttcgatt ccactcgtgc ccattatatg gtccgttggt
     5521 caaggggtta agacaccgcc ttttcacggc ggtaacacgg gttcgaatcc cgtacggact
     5581 atattattcg gaggattacc caagtccggc tgaagggaac ggtcttgaaa accgtcaggc
     5641 gtgtaaaagc gtgcgtgggt tcgaatccca catcctcctt actattatcg cgggatggag
     5701 cagctaggta gctcgtcggg ctcataaccc gaaggtcgta ggttcaaatc ctgctcccgc
     5761 aatttcttta agaatttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
     5821 gtgtcggcgg ttcgattccg tctcgcgcca ttaatttaat attatatttc ggagagatag
     5881 cgaagaggct aaacgcggcg gactgtaaat ccgctccttc gggttcgggg gttcgaatcc
     5941 ctctctctcc atacctttac gggcatagtt taaaggtaga actaaggtct ccaaaacctt
     6001 cagtgtgggt tcaattccta ctgcccgtgt tatatggcgg gtgtggtgaa gtggttaaca
     6061 catcagattg tggctctgac attcgggggt tcgattcccc tcactcgcct attttaatat
     6121 tggggtatcg ccaagcggta aggcaaggga ctttgactcc ctcatgcgtt ggttcgaatc
     6181 cagctacccc agttcaacta tatgccggcg tggcggaatt ggcagacgcg ctggactcaa
     6241 aatccagtgt ccgcaaggac gtgccggttc gaccccggcc gccggtatag tttaaaagac
     6301 aaggtttcta agccttgtct tttgttttat cttttctatt gctctttttc aagttgccaa
     6361 aaaattccat tttctaaaaa atatcgtcca atttgttcgc caatgtcttt tgtttactag
     6421 ggtataagtg ggaatatgta tctattgtag tcgtgataga ggcgtgtcca agacgttctt
     6481 ttacgactag ataatcttcc ccttgattga taagtaaaga cgcgtgagag tgccttaaat
     6541 cgtgtattcg tatctttttt agatagtcgt tcctagccag tatctgctta aattttttat
     6601 caatcatatt ttttgtcatt ggtagtggtg tgctttgaat tacttgaagg ttttctgtat
     6661 ctgttgtaaa ttctttaagt aagtcttttt gtttttcttt ccagtctgca agcattcttg
     6721 ctaatttatg attgattgta atacgtctgg tacctgctct agtttttgta ctattgatat
     6781 gatttgtacc gttaacaaag tatgctgttt ttgtgatgtg aatgctatta gtgattaagt
     6841 ttatatcgta ccaagttaac gcaactatct cgcccaatcg catgccagta aaaaaggcaa
     6901 tcgtaaagaa taggtcataa cttatttcat cacttgtaat aagttttcta aattcgttaa
     6961 attcttctac attccaaaaa tttatagttg gcttactaat aggtaatttt cttatatttt
     7021 caactgg
//
LOCUS       DBNFCK010000099         6970 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.099, whole genome shotgun sequence.
ACCESSION   DBNFCK010000099 DBNFCK010000000
VERSION     DBNFCK010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6970
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.099"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..292
                     /locus_tag="AC1PIW_001542"
     CDS             <1..292
                     /locus_tag="AC1PIW_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HIP6606453.1"
                     /translation="DRQQQYKWFDPIWWTLLPVLYMIFGLVNGLFIKIPIPDAKDSPF
                     AYFFLNVPKYGWTYVLTYAGVIFVAYLVCGFLLAGLKSINLKTSLSFGKNSQ"
     gene            491..1012
                     /gene="pyrR"
                     /locus_tag="AC1PIW_001543"
     CDS             491..1012
                     /gene="pyrR"
                     /locus_tag="AC1PIW_001543"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HIP6606454.1"
                     /translation="MKTKEIVDDMTMKRAITRITYEIIERNKNLDNIVLAGIKTRGVF
                     IAKRIQERLKQLEGIDVPLGELDTKPFRDDMKVEDDTTNMTANVNDRDVILVDDVLYT
                     GRTIRAAIDNIVSLGRPARVSLAVLVDRGHRELPIRADYVGKNIPTSRSEEIIVHMAE
                     IDGQDAVLLVEGA"
     gene            1068..1991
                     /locus_tag="AC1PIW_001544"
     CDS             1068..1991
                     /locus_tag="AC1PIW_001544"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HIP6606455.1"
                     /translation="MTITNGKVSLKHLVTMETLSNEEVLGLIQRGIAFKRGEKVELDR
                     KYYASNLFFEDSTRTHKSFEMAELRLDMGMIDFDARTSSVNKGETLCDTILTMSALGV
                     DICVIRHSEVDYYKQLIDSPTIQTSIVNGGDGSGQHPSQSLLDLMTIYEEFGTFDGLK
                     IAIAGDITHSRVAKSNMQILKRLGAEIFFAGPEEWYAEEFDVYGQHLNIDDIVETVDV
                     LMLLRVQHERHDGDGGFSKETYNRLHGLTEERYKRLKDTAIVMHPAPVNRDVEIDDSL
                     VEAPKSRIVRQMQNGVFVRMAILEAIVNGKA"
     gene            2239..3318
                     /locus_tag="AC1PIW_001545"
     CDS             2239..3318
                     /locus_tag="AC1PIW_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HIP6606456.1"
                     /translation="MSKRRLILENGTIFEGEAFGADIDVTGELVFSTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDFESIKPTCKGVVVSEWARRASNWRNQMTLDEFLK
                     AKKIPAISGIDTRALTKIIRQHGTMKATLANVGDSVEHLTDQLRATILPTNNIQQVST
                     KTAYPAPGVGRSVVLVDFGLKHSILRELAKRDCNVTVVPYDTTAEEILALNPDGVMLS
                     NGPGNPDDVPEALDMIRGILGKIPIFGICMGHQLFAKANGATTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVAREDLPECLMITHEEINDKSVEGVRHKYYPGFSVQFHPDA
                     APGPHDASYLFDEFMELMDSFKKGQ"
     gene            3678..6857
                     /gene="carB"
                     /locus_tag="AC1PIW_001546"
     CDS             3678..6857
                     /gene="carB"
                     /locus_tag="AC1PIW_001546"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIP6606457.1"
                     /translation="MPKRTDIKKIMVIGSGPIVIGQAAEFDYAGTQACLALKEEGYSV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDAFLPTLGGQTGLNMAMEL
                     SKAGILDELGVELLGTKLSAIDQAEDRDLFKQLMEDLNQPIPESTIVTTVDEALEFAA
                     EIGYPVIVRPAFTLGGTGGGMCANEEELREIAENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDAADNALVVCNMENFDPVGIHTGDSIVFAPTQTLSDIENQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     VNPVTGTTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVYHNEMPELSQVTDDQLVEKIVKAQDDRLFYLSEALRRGYTVEELAQ
                     LTKIDLFFLDKLLHILEIEQELATNFDNIDLLKKAKKYGFADRKIAELWGRTESYIRQ
                     LRTEHKIVPVYKMVDTCAAEFESATPYFYSTYEWENESIRSEKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTEFSVSDKLYFEPLTLEDVLNVIDL
                     EKPKGVIVQFGGQTAINLAEPLAKAGIPILGTQVADLDRAEDRDLFEKALKDLGIPQP
                     PGQTATNEEEALEAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPEH
                     PVLVDSYIVGRECEVDAISDGKDVLIPGIMEHIERAGVHSGDSMAVYPPQTLSKEVQA
                     TIADYTKRLAIGLNCIGMMNIQFIIKDETVYVIEVNPRASRTVPFLSKVTDIPMAQVA
                     TKLILGQSLAELGYQDGLYPESNQVHVKAPVFSFTKLAKVDSLLGPEMKSTGEIMGSD
                     LTLEKALYKAFEASYQHLAEFGNVVFTIADEDKEEVLGLAQRFYELGYGLLATAGTAT
                     FLTENGLAVTTVGKLGDDSCPDIPSLVRAGKVQAIINTVGKKRVADGDGQVIRSSAIE
                     GGIPLFTALDTAEAMVKVLESRSFMTQAI"
BASE COUNT         1914 a         1328 c         1703 g         2025 t
ORIGIN      
        1 agatcgtcag caacagtaca agtggtttga cccgatttgg tggacgcttt tgcctgttct
       61 ttacatgatt tttggtctag tgaatggtct tttcataaaa attccaatcc cagatgctaa
      121 ggacagtcct tttgcttatt tctttctcaa tgtccctaag tatggttgga cctatgtttt
      181 gacttatgct ggagtcatct ttgtcgccta tctagtctgc ggttttctat tggcgggact
      241 aaaatccata aaccttaaga cttcattgtc ttttggaaaa aattctcagt aaaatccgat
      301 aatttctata ttttttcaga aaaaaattgt caaggctgag attttatgct atactctgct
      361 taataaatcc tttaaaacct gtccagagag gcagacaagg agtcggatgt taagcaggtt
      421 ggttctaact agcctatatt ttcatagtct ccttgtataa ggagactttt tctatacttt
      481 tggaggtctt atgaagacaa aagaaatcgt tgacgatatg actatgaaac gggcaatcac
      541 ccgcatcact tatgaaatta tcgaacggaa taagaatttg gataatattg tccttgcagg
      601 gattaaaaca cggggtgttt tcattgcaaa gaggattcag gagagactta agcaattaga
      661 gggtatcgac gttccgttgg gtgagttgga taccaaaccg tttcgagatg acatgaaggt
      721 agaagatgat acgacaaata tgactgccaa tgtcaacgat cgtgatgtta tattagtaga
      781 tgatgtgctt tatacaggac gaactattcg tgctgctatt gacaacatag tgtccttggg
      841 ccgtccagct cgtgttagtt tagcggtact tgtggatcgt ggacacaggg agttgcctat
      901 tcgagcagat tatgttggga agaatatccc aactagccgt tctgaagaaa tcattgtgca
      961 catggctgaa atcgatggtc aagacgctgt tcttctggtt gaaggtgcat agtcgcttcg
     1021 tttacaaatt ctcattctac atctagatca taaaggtagg tatcatcatg acaattacaa
     1081 atggtaaagt ttcactcaaa cacttggtta caatggagac cctctcaaat gaagaagtat
     1141 tgggactcat tcaacgtggt attgctttta aacgcggtga aaaggtagag ttagatcgga
     1201 agtactatgc ttctaacctt ttctttgagg attcaactcg gacgcataag tcttttgaaa
     1261 tggcagagct tcgtttggat atgggaatga ttgactttga tgctcgtacc agctcagtca
     1321 ataagggtga aaccttgtgt gataccattt tgaccatgtc ggcacttggg gtagacatct
     1381 gcgtgattcg tcactcggaa gtggattatt acaagcaatt gattgatagt cctaccattc
     1441 agacttcaat tgtcaatggt ggtgatggtt caggtcaaca cccaagccaa tccctacttg
     1501 atttgatgac tatttatgaa gaatttggaa cttttgacgg cttgaagatt gccattgcag
     1561 gtgatattac acactcacgg gttgctaagt ccaatatgca aattttgaag cgtctgggag
     1621 ctgagatttt ctttgctgga ccggaggaat ggtacgcaga ggaatttgac gtctatggtc
     1681 agcatttgaa tattgatgat attgtggaaa cggtagatgt actgatgttg ctccgtgtgc
     1741 aacacgagcg tcatgatggt gatggtggtt tttctaaaga aacttataac cgtttgcatg
     1801 gtttaacaga ggagcgctac aaacgcttga aagatactgc aattgttatg catcctgccc
     1861 ctgttaaccg cgatgttgag attgatgatt ctctagttga agcacccaaa tcacgtattg
     1921 tccgtcaaat gcagaatggt gtatttgtcc ggatggcgat tttggaagca attgtcaatg
     1981 gcaaggctta aaagtaagct gttcaggtat cttagaactg agcagttaga aatttgttag
     2041 acttatctta tttaatgtcg cccagagagg cgacgaatag aaaggagaag gtacaggttc
     2101 gagtaagatt tgatttttat attcgaacac gccctaagga ctgtgtgaaa aagataaata
     2161 tcgacttgac gcctatagcg tcggcgccga atttcctatt ttcactttgt ccttttaacg
     2221 ggctttgtat catagattat gtcaaaaaga cgtttaattt tggaaaatgg cactattttt
     2281 gagggggagg ccttcggtgc tgatattgat gtgactggtg agttggtttt ctcaactggg
     2341 atgacaggtt atcaggaatc catcacggac cagtcttata atggtcagat tttaaccttt
     2401 acctatccct tggtaggaaa ttacgggatt aaccgtgatg atttcgagtc gatcaaaccg
     2461 acttgtaagg gagtggttgt tagcgagtgg gctcgtcgag caagtaattg gcgtaaccag
     2521 atgactttgg atgaattttt aaaggctaag aaaattccag caatttctgg aattgacaca
     2581 cgcgccctaa ctaaaattat tcgtcagcac gggaccatga aggcgacatt ggcaaatgtt
     2641 ggtgattccg ttgagcattt gacagaccag ttgagagcga ctattttgcc aaccaacaac
     2701 atccagcaag tatcgacaaa aacagcctat cctgcacctg gagttggacg tagtgttgtt
     2761 ttggttgatt ttggattgaa acattctatt cttagagagt tagccaagcg tgattgcaat
     2821 gtgacagttg tcccttatga tacgactgct gaagaaattc ttgccctcaa tccagacggt
     2881 gtcatgcttt caaacggtcc tggtaaccca gatgatgttc cagaagcttt ggacatgatt
     2941 cggggcattc tcggaaaaat tccaattttt ggtatctgta tggggcacca actctttgct
     3001 aaagcaaatg gggcaacgac ttataaaatg aagtttggtc accgtggttt caaccatgct
     3061 gttcgtgaaa ttgcgacagg tcgagtggac tttaccagtc agaaccacgg ttatgcagtg
     3121 gctcgtgaag atttacctga atgtttaatg attactcatg aagaaatcaa tgacaaatca
     3181 gtagaagggg tacgccacaa gtactatcca ggtttctctg ttcaattcca cccagatgct
     3241 gcacctggtc cacatgatgc cagctatttg tttgacgaat ttatggaatt gatggattcg
     3301 tttaaaaaag ggcagtaagg tttaactact atcgagctaa accttactgc gtcggtcaga
     3361 gcaagtcgga cttcatgcta acgcatgtgt cctcctacgc aaagtccaat gggacctttg
     3421 ctaactctga cactagttta gaatttcaat tattatcgaa ttgagattct aaacgtcgga
     3481 cttatcttat ttaatgtcgc tcagagaggc gacgaataga aaggagaagg tacaggttcg
     3541 aataagattt gattttcata ttcgaacacg ccctaaggac tgtgcgaaaa agataaacgt
     3601 cgtcttgatg cttgaagcat cagcgtcgtg tttcctattt tcgctttgtc ctcttaacgg
     3661 gctttgtatc atacattatg ccaaaacgta cggatattaa gaaaataatg gtgattgggt
     3721 ctggtccgat tgtgattggt caggctgcgg agtttgatta tgctgggact caggcttgtt
     3781 tagccttgaa ggaagaaggt tacagtgtgg tcttggtcaa ttcaaaccct gcgaccatta
     3841 tgacagacaa ggaaatagca gacaaggttt atattgagcc aattacgctt gaatttgtca
     3901 cacggatttt acggaaggag cgtccagatg ctttcttgcc aactcttggt ggtcagactg
     3961 gtctcaacat ggccatggaa ttgtctaagg ctggtatttt agatgagctt ggagttgagt
     4021 tgttgggaac taaactgtct gcaattgatc aggcagagga ccgcgacctc ttcaaacaac
     4081 tcatggaaga tctcaatcag cctattcctg agtcaacgat tgtgacaaca gttgatgagg
     4141 ctttggaatt tgctgctgaa attggttatc ctgtcattgt tcgaccagcc ttcacgctgg
     4201 gtggtactgg cggtggtatg tgtgccaatg aagaagaact acgtgaaata gcagaaaatg
     4261 gtctaaaatt gtcaccagtg actcagtgtt tgattgagcg ttccattgct ggtttcaaag
     4321 aaattgaata cgaagtgatg cgggatgcgg ctgacaatgc cttggtcgta tgtaacatgg
     4381 aaaactttga ccctgtggga attcatacag gagattctat cgtatttgcc ccaacgcaga
     4441 cgctttcgga tattgaaaac cagatgttgc gtgatgccag tctcagcatt attcgcgccc
     4501 tcaaaatcga aggtggctgt aatgtgcagt tggcattgga tccacatagt ttcaaatatt
     4561 atgtcattga agtaaaccca cgtgtgtccc gttcgtctgc cttggcatcc aaagcgacag
     4621 gttatcctat tgccaaattg gcagcaaaaa ttgcggttgg cttgaccttg gatgaaatgg
     4681 tgaaccctgt tacaggaaca acctatgcca tgtttgaacc agctcttgac tacgttgtgg
     4741 caaaaattcc acgtttccca tttgataagt ttgaaaaagg ggaacgccgc cttggaacgc
     4801 agatgaaggc aactggggag gtcatggcca ttgggcgtaa tatcgaagaa agtctgctca
     4861 aggcctgccg ttctttagaa atcggtgttt accacaatga aatgccagaa ctcagccaag
     4921 tgacagatga ccaattggtt gaaaagattg tcaaggcaca ggatgatcga ctcttctatc
     4981 tttcagaagc ccttcgacgt ggctataccg tagaagaact ggctcaattg acgaagattg
     5041 atcttttctt ccttgataag ctgcttcata tcttagaaat cgagcaggaa ttggcaacga
     5101 attttgataa tattgattta ttgaaaaaag ccaagaaata cggttttgca gaccgcaaga
     5161 ttgcggaact ctggggacgg acagaatcct acattcgtca actgcggaca gaacacaaga
     5221 ttgtccccgt ttacaagatg gtagatactt gtgcggctga gtttgaaagt gctacacctt
     5281 atttctattc aacttatgag tgggaaaatg agtccatccg ttcggagaaa gaatctgttt
     5341 tggtgctggg ttctggtcca attcgtatcg gacagggtgt ggagtttgac tatgcgacgg
     5401 tacattctgt caaggccatt caggcagctg gctacgaggc cattattatg aactcaaacc
     5461 ctgaaactgt gtcaactgag ttttcggttt cggacaagct ttatttcgag ccgttgacct
     5521 tggaagatgt tttgaatgtg atcgaccttg agaaaccaaa aggggttatc gttcagttcg
     5581 gtggccagac ggctatcaat ttggcagagc cattggccaa agcaggcatt ccaatcttgg
     5641 ggacacaggt ggcggatttg gatagagctg aagacaggga tttatttgaa aaagccctga
     5701 aagacctagg gattccacag ccaccaggcc aaaccgcaac caacgaagag gaagctcttg
     5761 aagcggcccg caaaattggt ttcccggtac ttgttcgtcc atcatatgtt ttgggcggtc
     5821 gtgctatgga gattgtcgaa aatgaagagg acttacgttc ttatatgaga actgcggtca
     5881 aggcttcgcc agagcaccca gttttggtgg attcttacat cgtcggtcgt gagtgtgaag
     5941 tggatgctat ttcagatggt aaggatgtct tgattccggg tattatggag catatcgagc
     6001 gcgctggggt tcactcaggt gactcgatgg ccgtttatcc gccacaaacc ttgtccaaag
     6061 aagtgcaggc gaccattgca gactacacca aacgcttggc aatcggtctc aactgtatcg
     6121 gtatgatgaa tatccagttt atcatcaagg atgaaacggt ctatgtgatt gaggtcaatc
     6181 ctcgtgccag tcgtacggtt ccattcttgt ccaaagtcac agacattcca atggctcagg
     6241 ttgcaaccaa attgattttg ggacaaagcc ttgcggaact tggctaccaa gacggtcttt
     6301 atcctgagag caaccaagtg cacgtcaagg caccggtctt ctcgttcacg aaactagcta
     6361 aagttgacag tttgttagga ccagagatga agtcaaccgg tgagattatg gggtcagact
     6421 tgacactgga aaaagcgctt tataaggctt tcgaggcaag ttatcagcac ctggctgagt
     6481 ttggcaacgt tgtctttacc attgcggatg aagacaagga agaagtgctt ggtctggctc
     6541 aacgtttcta tgaacttggt tatggcctac tggccacggc aggaacagcc acctttctaa
     6601 cagaaaatgg acttgctgta acaactgttg gtaaattagg cgatgattcc tgtccagata
     6661 ttcctagtct tgttcgggca ggtaaggttc aggctattat caatacggtt ggtaaaaaac
     6721 gggtggcaga tggtgacggt caagtcattc gaagctctgc tattgaagga ggaattccgc
     6781 tctttacggc cctcgataca gcagaagcta tggtcaaagt gctggagagt cgtagcttca
     6841 tgacacaggc tatatagaag atactatcag gatgagcttt gaattcgtcc tgattttttg
     6901 atatactgaa gagtatgaaa atgattgtaa caactagtct tgggatggat gaggggctgg
     6961 tctatagggc
//
LOCUS       DBNFCK010000100         6632 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.100, whole genome shotgun sequence.
ACCESSION   DBNFCK010000100 DBNFCK010000000
VERSION     DBNFCK010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6632
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.100"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..198)
                     /locus_tag="AC1PIW_001547"
     CDS             complement(<1..198)
                     /locus_tag="AC1PIW_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HIP6606458.1"
                     /translation="MKQGKQISTKQRWMRYSAYMLISAILGGFIGFFGILIFKFSLPS
                     YLNLDNFLICLRIITFLIFAGT"
     gene            complement(195..410)
                     /locus_tag="AC1PIW_001548"
     CDS             complement(195..410)
                     /locus_tag="AC1PIW_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIP6606459.1"
                     /translation="MEYVLKNRLKELRARDGLNQTELAKLAEVSRQTISLIERGEYTP
                     SVVIAMRIAQIFNENVENVFQLVEVEE"
     gene            complement(423..989)
                     /locus_tag="AC1PIW_001549"
     CDS             complement(423..989)
                     /locus_tag="AC1PIW_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HIP6606460.1"
                     /translation="MEAKKKLYEVNIMENKKFVWGLNILAVVLIFPFAFVFGMIVSWL
                     IANGSQYSELSVSEVWLLIPLYILLIVVHEAIHGIFFKVFCPENPVKYGFKWKSGMAY
                     ATSPGSLYNRMQMLVISLAPFVVISLVLTMVAGFGMMNITLYFMLATMHAASCAGDFY
                     YTYLLLVKFAKGNIAVEDTETGLIIYQA"
     gene            complement(1175..1399)
                     /locus_tag="AC1PIW_001550"
     CDS             complement(1175..1399)
                     /locus_tag="AC1PIW_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606461.1"
                     /translation="MKTSSNKKGILITGTSVVRGLEVIIALFLIYAFVQILGFGQPID
                     MVPLLMMVSSLFILKTTLGREKEKKNEQDS"
     gene            complement(1433..1633)
                     /locus_tag="AC1PIW_001551"
     CDS             complement(1433..1633)
                     /locus_tag="AC1PIW_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606462.1"
                     /translation="MKKINNSDMAGFVQLIAIIYLVFVLLADIMKWQQLDQTHVTLAI
                     CAIAVTTGSLKKLGGRKESKVK"
     gene            complement(1842..4460)
                     /gene="alaS"
                     /locus_tag="AC1PIW_001552"
     CDS             complement(1842..4460)
                     /gene="alaS"
                     /locus_tag="AC1PIW_001552"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIP6606463.1"
                     /translation="MKNLSSAQIRQMWLDFWKSKGHSVEPSANLVPVNDPTLLWINSG
                     VATLKKYFDGSVIPDNPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAIEWGFELLTSPEWFAFPKDKLYMTYYPDDMDSYNRWIALGVEPSHLIPLEDNFWEI
                     GAGPSGPDTEIFFDRGTDFDPENIGIRLLEEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPNKNIDTGAGLERLAAIFQGAKTNFETDLFLPIIREVEKISGKTYDQDGDNMS
                     FKVIADHIRALSFAIGDGALPGNEGRGYVLRRLLRRASMHGQRLGITEPFLYKLVETV
                     GNIMESYYPEVLEKRAFIEKIVKSEEESFARTIHTGSQFAEQLMDKLAAEGKSEIDGR
                     DIFKLYDTYGFQVELTEELAEHRGMTLDIAGFEAAMKEQQDRARASVVKGGSMGMQNE
                     TLAAITEESTFVYGQTALEASLSVIVADNARTEAVSEGQALLVFDQTPFYAEMGGQVA
                     DHGFVKNATGDIVATVIDVQKAPNGQPLHTVELSASISVGQTYTLEIETKRRKGVEKN
                     HTATHLLHAALHNIIGEHATQAGSLNEQDFLRFDFTHFEAVTAEELRRIEEEVNEQIW
                     NAIPVVTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSVELCGGTHVGNTAEIGI
                     FKILKEEGIGSGTRRIIAVTGREAFLAYRDQEDALKEVAATIKSPQIKEVPNKVESLA
                     QQVRDLQKENAALKEKAAAAAAGDVFKNVKDANGIRYIASQVQVSDAGALRTFADNWK
                     QKDYSDVLVLVAAIGDKVNVLVASKSSDVHAGNLIKVLAPIVAGRGGGKPDMAMAGGS
                     DASAIQTLLNSVADNL"
     gene            complement(4740..5225)
                     /locus_tag="AC1PIW_001553"
     CDS             complement(4740..5225)
                     /locus_tag="AC1PIW_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HIP6606464.1"
                     /translation="MKQFQVKQRFTFGGEKFDIRDSFGNLAYQVEGSFLEIPKRFIVT
                     NSRGGEVCQITKKFLTFLPQFTIDMVEGHSFYLQKEFTFFKDRYTVENLGLILDGNIW
                     DLDFRLKRPDGMLVAEISKELFHLTSHYSVTVLEDSCADLVISLVVAIDYIEMMEDAS
                     T"
     gene            complement(5493..6494)
                     /gene="prsA"
                     /locus_tag="AC1PIW_001554"
     CDS             complement(5493..6494)
                     /gene="prsA"
                     /locus_tag="AC1PIW_001554"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HIP6606465.1"
                     /translation="MKQTKKILAGAVTLFAAVTLAACSNAADKDIITMKGNTITVSEF
                     YEKVKTNSQAQQVLLSMVISNVFENQYGDKVSAEEVNKEYDKKAEQLGASFNAALSSA
                     GLTEESYKEQIRTNKLVEYAVKQAAEKELTDENYKAAYDAYTPEVTARVIKLADEAKA
                     KEVLAAAQAEGADFAQLAKDNSTDTTTKDNGGEVKFDSTSTTVPAEVQKAVFALDAGQ
                     VGASVISSVDMKTYTTSYYVVKLDAKSEKSAKWEDYKDKLKEIILAQKQRDSSFVATV
                     LKEALQKANVKVKDSAFQNLLSQYVTTEESSSSSTKSSSSSTESSSSTTESSSSSGQ"
BASE COUNT         1951 a         1472 c         1217 g         1992 t
ORIGIN      
        1 ggttccagcg aaaattaaaa atgtaataat cctcaaacaa attagaaaat tatctaaatt
       61 gagataagaa ggtaggctga atttaaagat taatattcca aagaaaccga taaagccacc
      121 caaaattgct gaaatcagca tatacgctga atatctcatc catctttgtt tcgtcgaaat
      181 ttgtttcccc tgtttcattc ttctacctcc actaattgaa agacattctc cacattttca
      241 ttaaaaattt gtgcaatccg catagcaatg accaccgagg gtgtgtattc tcctcgttca
      301 atcaagctaa tagtctgccg tgatacttct gccaactttg ctagctctgt ctgattgaga
      361 ccatctctgg ctcgcaattc cttgagccga ttttttaaga catattccat aaattttcct
      421 ttctaggctt ggtagataat caagcctgtt tctgtgtctt cgacagctat atttcccttg
      481 gcaaacttga ccaataagag ataggtatag tagaaatcac ctgcacaaga ggcagcgtgc
      541 atggttgcca gcatgaagta aagagtaata ttcatcatcc caaaacctgc taccatagtc
      601 aagaccaaac taatcacaac aaagggtgct agggaaatca ccagcatttg catccgattg
      661 tagagggaac ctggactggt agcataggcc atgcccgatt tccatttaaa cccgtatttg
      721 acaggatttt caggacaaaa aaccttgaag aagatgccgt ggatagcctc atgcacaact
      781 attaacagaa tatagagtgg gataagcaac catacctccg acacggatag ctccgagtat
      841 tgcgaaccgt tagctatcaa ccaactgact atcattccaa atacgaaggc aaatggaaag
      901 atgagtacca cagcaagaat attcaatccc cacacaaatt tcttattttc catgatatta
      961 acttcatata atttcttttt agcttccatt tctcttctct ttagatttca ttttgtaaaa
     1021 tagcagagct gtcaccaatg tcgccagcaa tggaggaaat agataacttc tctcagtcaa
     1081 actcatctca aaacctaaca aagataaaac tttaaacacc aaaatattta tcaagaagag
     1141 tacgaaaaat agtaggacta ctttcactcc gtttttaact gtcttgttca ttcttctttt
     1201 ccttttctct gccgagagtg gttttcaaaa tgaataggga actcaccatc atcaagagtg
     1261 gcaccatatc tataggctga ccaaatccaa gaatttgaac gaaagcatat atcaaaaata
     1321 gagcaattat aacttccaaa cctcgcacaa cacttgttcc tgtaatcaaa attccttttt
     1381 tattgctact ggttttcata attttccacc acctactact cgactaattt tactacttta
     1441 cctttgactc ttttctgcca cctagtttct tcaagcttcc agttgtcact gcgatggcac
     1501 aaattgcaag agtcacatga gtctgatcca actgctgcca cttcataatg tcagctagta
     1561 ggacaaaaac tagatagatg atcgcgataa gttgtacaaa acctgccatg tcactattgt
     1621 ttatcttttt catatatagt tcctactttc tgtttattta ccagatggat tcgttttcat
     1681 ctttatctca atatcattgt aacacttact tttctttttg acaactatca ttgtcaaaaa
     1741 aataataatt tttgtcaaaa agtttatgaa gttcgtcaaa atgactagaa taacatgcaa
     1801 aaaactcttc tacccaatgg ataaaagagt ttattgagcg attatagatt atcagctacg
     1861 ctgttcaaga gggtttggat tgcagaggca tcgctaccac ctgccatggc catatctggc
     1921 ttaccaccac cacgacctgc tacgattggc gcaaggactt tgatgaggtt acctgcatga
     1981 acatcgcttg acttgcttgc cacaaggaca ttgaccttgt cgccgatggc cgcaaccagc
     2041 accaagacat ctgagtagtc tttctgcttc cagttgtcag cgaaggtacg gagggcacct
     2101 gcatctgaaa cttggacttg gctggcaatg tagcgaatgc cgttagcatc tttgacattt
     2161 ttgaagacat caccagcagc ggctgccgct gctttttctt tcagagctgc attttctttt
     2221 tgtaggtcac gaacttgttg tgcaagactt tcaaccttgt ttggcacttc cttgatttgt
     2281 ggtgacttga tggttgccgc tacttctttg agggcatctt cttggtcacg ataggcaagg
     2341 aaggcttcac gacctgttac cgcaatgata cggcgagtac ctgaaccgat accttcttct
     2401 ttcaaaatct tgaagatacc gatttcagcg gtgttgccaa cgtgggtacc accacagagt
     2461 tcgacagaat agtcaccaat tgtcaccaca cggacttctt taccgtattt ttcaccgaag
     2521 agggccatag cacccatttc cttagctgtg tcaatatcgg tttcaaccgt tacaactgga
     2581 atggcattcc aaatttgctc attgacttct tcttcaatac gacgaagttc ttccgcagta
     2641 actgcctcaa agtgcgtaaa gtcaaagcgg aggaagtctt gctcgttcaa ggaaccagcc
     2701 tgtgttgcat gctcgccgat aatattgtga agagctgcat ggagcaagtg agttgctgtg
     2761 tggttcttct caacaccctt acggcgtttt gtttcgattt caagtgtgta ggtttgacca
     2821 actgaaatgc ttgcggacag ctcaactgtg tgtaatggct gaccatttgg tgctttctgc
     2881 acatcgatga cagtcgcaac aatgtctcca gtagcatttt tcacaaaacc atggtctgca
     2941 acctgaccac ccatttctgc atagaatggt gtttggtcaa agacaaggag ggcttgacct
     3001 tctgagactg cttctgtacg cgcattatca gccacaatga cagataagct tgcttcaaga
     3061 gctgtttggc cataaacaaa ggtcgactct tctgtgatag cagccagggt ttcattttgc
     3121 atccccattg aaccaccttt gacaactgaa gcacgggcac ggtcttgttg ctctttcata
     3181 gccgcttcaa aaccagcaat gtcaagggtc ataccacggt gctcagccaa ttcttctgtc
     3241 aattctactt ggaaaccata ggtatcatag agtttgaaaa tgtcacgacc atcaatctca
     3301 gacttacctt ctgctgccaa tttatccatc aattgctcag caaattgtga acctgtatgg
     3361 atagtacgag caaatgactc ttcttcactc ttaacaatct tctcaatgaa ggcacgtttt
     3421 tcaagtactt ctggatagta actttccatg atattaccaa ctgtctccac caatttgtac
     3481 aagaatggct ctgtaatgcc caaacgttga ccatgcatag aagcacgacg aaggagacga
     3541 cgaaggacat aaccacgacc ttcatttcca ggaagggcac cgtcaccaat agcaaaagaa
     3601 agggcacgga tgtggtctgc aataaccttg aagctcatgt tgtcgccatc ttggtcatag
     3661 gtcttaccag aaatcttttc tacttcacga atgattggca agaagagatc cgtttcaaag
     3721 ttggtcttag ccccttggaa aatagctgct aaacgctcca aacctgcgcc cgtatcgatg
     3781 tttttgtttg gcaattcctt gtactcagag cgaggaacag ctgggtctgc gttgaactgg
     3841 gacaatacga tattccaaat ctcaatgtaa cggtcatttt caatatcttc ttccaagaga
     3901 cggataccaa tgttttcagg gtcaaagtca gtaccacggt caaagaaaat ctccgtatct
     3961 ggaccagaag gacctgcacc gatttcccag aagttatctt caagcggaat caagtggctt
     4021 ggctcaacac caagggcaat ccaacgattg tatgaatcca tatcatcagg atagtaagtc
     4081 atgtagagct tgtcttttgg aaaagcaaac cattctgggc ttgtcaagag ctcaaagccc
     4141 cattcaatcg cttcatcacg gaaataatca ccaatagaga agttaccaag catttcaaac
     4201 atggtgtggt ggcgggctgt cttaccaacg ttctcgatgt cgttggtacg gattgatttt
     4261 tgggcatttg taatccgtgg gttatctgga atgacagagc catcaaagta tttcttgagg
     4321 gttgccacac cagagttgat ccacaaaaga gtgggatcgt ttacagggac aagatttgct
     4381 gaaggctcaa cagagtgacc ctttgatttc cagaaatcca accacatttg acgaatttga
     4441 gcagaagata aatttttcat aaactaagat accaagcccg attagaaggc gactgaaaaa
     4501 taggaaactg acaggaaatc ttgatttcct aggcagttta tcttttttcc gagccttccg
     4561 agcgggttca atttaacgaa acagttaaat taaacatttc ctttcatata gtaataattg
     4621 aggggaaagt ccgtagcccg agttcatttc agaataaact cgataccctt tcctttcata
     4681 agtgatagtt aggcgggttc aatttaacgg accagttaaa ctgaatgatt atttcacgat
     4741 taagtcgatg catcttccat catttcgatg tagtcaattg cgaccactaa ggaaatgacc
     4801 aaatctgcac aggagtcctc caatacagta acggagtaat gagaagttaa atgaaagagt
     4861 tctttggaga tttcagctac gagcatacca tctggccttt tcaagcgaaa atccaaatcc
     4921 cagatatttc cgtctaggat taaacctaga ttttccacag tatagcgatc cttgaaaaaa
     4981 gtaaattcct tttgcaagta gaaagaatgt ccctctacca tatcaatggt aaactggggc
     5041 aggaaggtca aaaacttctt agtaatctga caaacttccc cacctctgga attggttacg
     5101 ataaatcgtt ttggaatttc gagaaaagat ccctccactt gataagcaag attcccaaaa
     5161 ctatcacgaa tatcaaattt ttcaccacca aaggtaaagc gctgtttcac ttgaaattgc
     5221 ttcatgataa cctcccccaa aaaaataaca agaaatcaaa cgaagggcga aaaaccgcgg
     5281 taccaccttc attcaatgac ttgtcattct catttaagaa actgtatcca caagcaaaaa
     5341 acttttatat ataagccaca aactgtgaat acaggctcta ctattcaatt gtaatattaa
     5401 gtagcaaaat acttggctta gatggctcgc aacaaccgcc aattttctga actaaggtgt
     5461 caagtaccaa ttcttaataa gtaaatcgtc aattactgac ctgatgaact agaagattct
     5521 gttgtagaag aagaactttc agtcgacgaa gagctgctct tcgttgagga agaactgctt
     5581 tcttctgttg tcacatactg tgaaagaaga ttttggaaag cactgtcttt aaccttaacg
     5641 tttgcttttt ggagtgcttc tttcagcaca gtagcaacaa atgatgaatc tctttgcttt
     5701 tgtgccaaaa taatttcttt cagcttatct ttataatctt cccatttagc agatttttct
     5761 gatttagcat ccaattttac aacgtaataa ctcgttgtat acgtcttcat atctaccgaa
     5821 gaaatcaccg acgctccgac ttgacctgca tccaaggcaa ataccgcttt ctgtacctct
     5881 gctggaacag ttgttgaagt tgaatcgaat ttaacttcgc ctccattgtc ttttgtagta
     5941 gtatccgtgg aattgtcttt cgccaattga gcaaaatctg caccttcagc ttgtgcagca
     6001 gctaaaactt cttttgcttt agcttcgtct gccaacttga tgacacgtgc tgttacttca
     6061 ggtgtatagg cgtcataagc tgccttgtag ttttcatctg tcaattcttt ctcagcagct
     6121 tgttttacag catactcaac caatttattg gtacgaattt gttccttata ggattcttct
     6181 gtcaatcctg ccgaagaaag tgccgcgtta aaagaagcac caagttgttc tgccttctta
     6241 tcatattcct tatttacttc ttctgcagag actttgtcac catactgatt ttcaaataca
     6301 ttactgataa ccattgacag caaaacttgt tgagcttgtg agtttgtttt tactttctca
     6361 taaaactcag acacagtaat cgtgttacct ttcatcgtaa tgatatcttt atcagctgca
     6421 ttggaacaag cagcgagtgt tactgcagca aagagtgtta ctgcacctgc gagaattttt
     6481 ttagtttgtt tcattaaaga ctctctcctt ttttcattaa cccctttatt ataacacaaa
     6541 agcttaaaaa cttcttaaat ttctatatgt tttaagtcaa cattctctac atttttcctc
     6601 aacataagga gaccgtcact catggaaaca ag
//
LOCUS       DBNFCK010000101         6558 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.101, whole genome shotgun sequence.
ACCESSION   DBNFCK010000101 DBNFCK010000000
VERSION     DBNFCK010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6558
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.101"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            13..813
                     /locus_tag="AC1PIW_001555"
     CDS             13..813
                     /locus_tag="AC1PIW_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIP6606466.1"
                     /translation="MKKILALAATVLAGLTLAACSSTSSQSALDKIKEKGTLVVATSP
                     DYAPFEFQALVDGKNEVVGADIMLAQKIADELGVKLEVSAMSFDNVLSSVQNGKADIA
                     IAGLSYSEERAKVFDFSESYYQISDVLLIKKDSANSLTSIDAMSGKTLAVQKGSTQES
                     YAQENISQANLISLTLMGEAVNELKSGKVDAILMDSPVAAGYVSQNSDLAVASVEFPT
                     IDENSKVIALPKGSTELKTAIDKVIAEVKASGEFDTFLEKAATYTSVE"
     gene            complement(941..2779)
                     /gene="glgB"
                     /locus_tag="AC1PIW_001556"
     CDS             complement(941..2779)
                     /gene="glgB"
                     /locus_tag="AC1PIW_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HIP6606467.1"
                     /translation="MTEFTDLDLYYMNTGEHTTLYEKMGAHIIKERNKIIGTQFRVYA
                     PNAREVFLVGNFNEWQRSHSMEREIDGVFQLYVDGLKSLEDYKYLIITHDGREIYKAD
                     PYAFFSQVRPDTASTTYNSRYKFKDDIWMYNRVNYDFKEQPVSIYEVHLGSWKQKFAN
                     KNEGGAPVEAFNSYKEITPLLIEHIKENNYTHIELLPITEHPLDASWGYQVTGYYSPT
                     SRYGKPDELKYLVDQCHQAGIGVILDWVPLHFCKDAHGLYQFDGSWLYEYSYEQDREN
                     HQWGTANFDLGKGLTRSFLLSNLKYWLEYFHFDGIRVDALSYLLYWRGETDEDKINHA
                     AIDFIKRVNAMVHTDYKGVLMIAEDSSSFPKVTHPLEDGGIGFDMKWDLGWMNDTLKF
                     VERPAIYRKYHSNEITFGMYYNQNEQFLLPLSHDEVVHGKHSIIEKMNGDYEDKFHLA
                     RVYYSFYFAHPGKKLLFMGNEWGHIREWHEYTEMDWGLLQFPIHHSFYQMMKTLSTFY
                     KEHDAFWKYDYQAYEKGFNWVKIDEEASLYAFSRTSDVQEILTIHNFNDQELFDVHLD
                     LPADSEYKLVFSSNAIPMDTFSLYPDENGARITVPRLTSFYLERVK"
     gene            complement(2852..4297)
                     /gene="glgA"
                     /locus_tag="AC1PIW_001557"
     CDS             complement(2852..4297)
                     /gene="glgA"
                     /locus_tag="AC1PIW_001557"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - alpha-1,4-glucan
                     glucosyltransferase (UDP-glucose donor) activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HIP6606468.1"
                     /translation="MTKKSVLFVASEGLPFIKTGGLADVIGSLPKELVKQGLDVRVVL
                     PLYKKIAVNNHSDFDYVSSFDVRAGDIQSMANVYSQVVDGVTFYFIEHRDFFERDELY
                     GYDDDAMRFGYFQHATCRLLEALHFFPDVMHTHDWHTAALPFLCRTFYSYREEFRNIK
                     HVFTIHNLAFQGIFHKQALWSALGMDYSYYLDGIARFHDECISFMKLGILYADKVTTV
                     SETYAREILTEEFGENMQHVLELRKYDLVGIVNGIDYDSWDSQTDQYLVRNYSLETIE
                     SKKANKLALQSQFALPQDENVLLIGIVSRLTWQKGFYLLTEVLGHLLQAHVQFVILGN
                     GEEDIENAFNHFKHAYPDKFAFYRGYNEPLAHQIYAASDLFLMPSMFEPCGISQLISM
                     HYGTLPLVRETGGLVDTVAPYNMSTKEGTGFSFGGRDAYNMRQVYDLALQTYYDRPDD
                     WYAMVEQAMKRDFSWTASAEKYIWLYREISG"
     gene            complement(4306..5421)
                     /gene="glgD"
                     /locus_tag="AC1PIW_001558"
                     /pseudo
     CDS             complement(4306..5421)
                     /gene="glgD"
                     /locus_tag="AC1PIW_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922501.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
     gene            complement(5434..>6558)
                     /locus_tag="AC1PIW_001559"
     CDS             complement(5434..>6558)
                     /locus_tag="AC1PIW_001559"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HIP6606469.1"
                     /translation="AQNKMLAMILAGGRGTRLEGLTKKVAKPAVAFGGKYRIIDFPLS
                     NCANSGIDIVGVLTQYEPVLLNSYVAQSQRWGLDVQGSGVFVLPPSEKIEGFGLYKGT
                     ADAITQNIDFIDLHDPEHVLILSGDHIYKMNYDKLLDTHIQKKADATIAVIEVPIKEA
                     SRFGIMNTDEDYRIEEFEEKPENPKSNLASMGIYIFTWKTLKKYLQEDDKLDTSSHDF
                     GHDIIPKYLEDGRTLIAHPFRGYWKDVGTVNSLWESNMDLIDHAGDLDLSDRSWRIYS
                     EDKGSPAQVIGATATVKSAYIDKGAIIDGTVEHSVISTDVQVNQNAVVKNSVILPGAI
                     IGEGVELDYVIVAENIKIADGVKLTGDIDHILLIDKNVTK"
BASE COUNT         1937 a         1271 c         1349 g         2001 t
ORIGIN      
        1 tatttaagaa atatgaaaaa aatcttggca ttagcagcaa cagttttagc tggactgaca
       61 ttggcagctt gttcttcgac gtcatcacaa tccgcattag ataaaattaa agaaaaagga
      121 acattagtag tggcaactag tccagactat gctccattcg agtttcaggc actagttgat
      181 ggaaagaatg aagttgttgg tgcagatatt atgttggctc aaaaaatagc cgacgaactt
      241 ggggtcaagc ttgaagtttc agcaatgagt tttgacaatg tattgtctag cgttcagaat
      301 ggcaaagctg atattgcaat tgctggtcta tcttattcgg aagagcgtgc aaaagttttt
      361 gatttctctg aaagttacta tcaaatttct gatgtattac ttatcaaaaa agatagcgcc
      421 aattcactca cttcaattga tgctatgagc gggaaaactc tcgcggtaca aaagggttca
      481 acacaagaat cctatgcaca ggaaaacatt agtcaagcta acctaatttc tttaaccttg
      541 atgggtgaag ctgtaaatga gctcaaatca ggaaaagttg atgctatttt aatggattct
      601 ccagttgctg ctggttatgt ttcacagaat tcagatttag ccgttgcatc cgttgaattt
      661 ccaacgattg atgagaattc gaaagtcatt gctttgccaa aaggtagtac agaactaaaa
      721 actgcgatag acaaggttat cgcagaagta aaagcaagtg gtgagtttga tactttcctt
      781 gagaaagctg caacatatac aagtgttgaa taagagaaat gaacaatcat cggaaacggt
      841 ggttgttttt gcatgcaaaa aagaatggag taagtgagcc acacaccaaa agttagacag
      901 aaaatttggg tcggttcaat actccatttt ttatagataa ttatttcaca cgctctagat
      961 aaaaactggt caaacgtggt acagtaattc tggctccatt ttcatcggga tagaggctaa
     1021 atgtgtccat tggaatagca ttagatgaga agacaagttt atattcgctg tcagctggca
     1081 aatccaagtg tacatcaaat agttcttgat cattaaagtt atgaatagtc aagatttctt
     1141 gcacatcact agtgcgagaa aaagcataca ggcttgcttc ttcatctatt tttacccaat
     1201 taaatccttt ttcataggct tggtagtcat atttccagaa agcatcatgt tctttataga
     1261 aagtagatag tgttttcatc atttgataga aagaatggtg gattggaaat tgtagcagtc
     1321 cccagtccat ttctgtatat tcgtgccatt cacggatgtg accccattca tttcccatga
     1381 agagtaattt ctttccagga tgggcaaagt aaaagctgta gtagactcga gccagatgga
     1441 acttatcttc gtagtcgcca ttcatcttct cgataatgga gtgtttccca tgaacaactt
     1501 cgtcgtgcga aagtggtagt aagaattgct cgttttggtt atagtacata ccaaaagtga
     1561 tttcatttga gtgatatttt cgataaatag cagggcgttc cacgaatttt agtgtatcat
     1621 tcatccagcc cagatcccat ttcatatcaa atccaatacc gccatcttct agaggatgtg
     1681 tgactttagg gaagcttgaa gagtcttcag caatcatcaa gactccctta tagtcggtat
     1741 ggaccatggc attgacacgt ttgataaagt caattgcggc atgatttatc ttatcttcat
     1801 ctgtttcacc acgccaatag aggagataag atagtgcatc aacacggatg ccatcaaaat
     1861 ggaaatattc caaccagtat tttaaattag aaagaaggaa agaacgagtc agtccttttc
     1921 caaggtcaaa attagcagtc ccccactggt gattttcacg gtcttgttcg taggagtatt
     1981 cgtatagcca actaccatca aactggtaga gcccatgagc atctttacag aaatgaagtg
     2041 gtacccagtc aagaatcact ccgattcctg cttggtgaca ttggtcaact aaatacttaa
     2101 gttcatctgg cttgccatag cgactagttg gactgtagta gcctgttacc tgatagcccc
     2161 aagacgcatc gagaggatgc tcggtaattg gcaataactc aatatgtgtg tagttatttt
     2221 ccttgatatg ttcgatgaga agtggagtaa tttccttata actattgaaa gcttctacag
     2281 gagctcctcc ttcattttta ttagcaaatt tttgcttcca cgaacctaag tgcacctcgt
     2341 agatggatac tggctgttct ttaaaatcat agttaacacg attatacatc cagatgtcat
     2401 ctttgaattt ataacgcgaa ttgtaggttg tagaggcagt gtctggacga acttggctga
     2461 aaaatgcata agggtcagcc ttatatattt cacgcccatc gtgtgtgata atcaggtatt
     2521 tataatcttc aagggatttt aatccgtcta cataaagttg aaatactcca tcaatttcac
     2581 gctccataga gtgactgcgt tgccactcgt taaagttgcc aactaagaat acttctctgg
     2641 catttggggc atagacacga aattgggttc cgataatttt gtttcgttct tttataatat
     2701 gagcacccat tttttcgtaa agagtggtat gctcaccagt attcatataa tataaatcta
     2761 ggtcagtaaa ttctgtcata gcaatctcca ttgccgaaaa ggagtggact agggtcccac
     2821 tccgattcag tatatttttt catcttattt cctaaccact aatttcacgg tagagccaga
     2881 tgtatttctc tgccgaagct gtccaactaa aatcacgctt catagcttgt tcaaccatgg
     2941 cataccagtc atcgggacgg tcatagtagg tttgaagggc taaatcatag acttgtcgca
     3001 tattgtaagc gtcacgccct ccaaagctaa agccagttcc ttccttggta gacatattgt
     3061 atggtgctac agtatcaacc aagccacctg tttcacgcac tagagggagt gttccgtagt
     3121 gcattgaaat caactggcta atgccgcatg gttcaaacat agaaggcata aggaagagat
     3181 cacttgctgc ataaatttgg tgggcaagtg gttcattgta accacggtag aatgcaaatt
     3241 tatcaggata agcatgttta aagtgattaa aggcattttc aatgtcttcc tcaccattgc
     3301 ctaagataac aaattggaca tgggcttgaa gaagatggcc aagtacctcg gtcaagaggt
     3361 agaacccttt ttgccatgtc aaacgagaga cgatacctat caataggaca ttctcatctt
     3421 gtggaagggc aaattgtgat tgcagggcca gtttattagc ctttttactt tcaatcgttt
     3481 caagactata atttctcaca aggtactgat ctgtttgact atcccaactg tcatagtcga
     3541 taccattgac aataccaacc aagtcatatt tgcgcaactc tagtacatgt tgcatgtttt
     3601 cgccgaattc ttccgttagg atttcgcgag cataagtttc agaaactgta gtaaccttgt
     3661 cagcgtatag gatacccagc ttcatgaagc tgatacattc atcatgaaaa cgtgcaatac
     3721 catccaaata gtagctatag tccatgccaa gcgctgacca aagtgcttgt ttgtgaaaaa
     3781 taccctgaaa agccaagtta tgaatagtga atacatgctt gatattgcgg aattcttcgc
     3841 ggtagctata gaaagtacgg caaaggaatg gaagggcagc agtgtgccag tcatgtgtgt
     3901 gcatgacatc cgggaagaaa tgaagtgctt ctaacagacg acatgtagca tgttggaaat
     3961 agccaaaacg catggcatca tcgtcgtatc catatagttc atcgcgttca aagaaatcgc
     4021 ggtgttcaat aaaatagaat gttactccat caacaacctg actgtaaaca tttgccatag
     4081 attggatgtc gccagcccgc acatcaaaac ttgatacgta atcgaaatca gagtgattgt
     4141 ttactgcaat ttttttgtag agtggtagaa cgactcggac atctaaaccc tgttttacca
     4201 attcttttgg taaagagcca ataacatcag ccaatccgcc agtcttgatg aatggaagtc
     4261 cctcggatgc tacaaagaga acagattttt ttgtcatgtt agctcctaaa tgatttggtg
     4321 tggtttcaag tatgaaggcg aagtttcgct tcccttgatg tcaattgggt gaataatttt
     4381 agttgcttta tcgataattg cattttcaat attcgccccg ctcttgattg tcacgccatt
     4441 gagaatgatt gagtttttga taacaacacc ttcttcaatg ataacatcgc ggtcaatgac
     4501 tgaattttcg acttgaccat tgatattcgc tccatttgaa attaggctac gtttgacctc
     4561 tgccaatgga ccatataaac atggtgagct atcacttgtt tgagtatgga ttggccaatc
     4621 atgtttgaag aggagtttgc gggtatcttg gtcaatcaat tcaatttgag tattgaagta
     4681 ggaagctgtc gtattgatac atgcgacata atcacgatgt gcatagccca taatcttgta
     4741 ctggtcaact gtatcacgta aaatatcttt taaccagtag agagacgaca ctttgttagc
     4801 acgtttaatc aagtcgataa atgtagtccg tttcataatg taggcttcaa gggaaacggg
     4861 acggttttta tatttaccat ggttttcctc aaaagctacg acacgacggt cttcaccaaa
     4921 tttgagagtt tgacaaccga tgaaattttc tttagcatct gaaacgtttt tataaagaac
     4981 ggtaatatca gcatccgttg cgatatggtc atccatgact tttgaaaaat cctggctata
     5041 gatgaagtaa gatggagcaa ctagcacata atcttttgta gaattttcga tgtagtggat
     5101 gttttctgtg aaggcattaa tatcgtgacg atagacacta tttgggtctg ttgcgctatt
     5161 gagcaagcga agggtaccgc gtttgctgtt gatattatag tgtttccctg taccaacgtg
     5221 ctgaattgtt gaacgcattt cggcaggcat gtatacttag aattctgtaa ttccagagtt
     5281 tgacatattg gatagagcaa agtcaattag gcgataacga cctaagaaac cgaaagcttg
     5341 tacgccacgg tgcttcatca cgtcaccaaa atgtacgttg tttccttcaa tatttacaat
     5401 tccgagagta tttcttagca tagcagtcct ttcttacttg gtcacattct tgtcaatcaa
     5461 gaggatatgg tcgatgtctc ctgtgagttt aactccgtcg gcaattttaa tattttcagc
     5521 gacgatgacg tagtcaagtt caacaccttc gccgataata gcaccaggta ggatgacaga
     5581 attttttact acggcatttt ggttgacttg cacatctgtt gaaataacag aatgttctac
     5641 agtaccatcg atgattgccc ctttatcaat ataggcagat ttaaccgttg cagttgcacc
     5701 aatgacttgg gcaggagatc ccttatcctc tgaatagatt ctccatgagc ggtcagaaag
     5761 gtccaaatct ccagcgtgat caatcaagtc catattggat tcccagaggc tgttaacggt
     5821 acctacgtct ttccaataac cacggaatgg atgggcaatg agagtgcgac catcttctaa
     5881 gtatttcgga atgatgtcgt gcccaaaatc gtgagatgaa gtgtccaatt tgtcatcttc
     5941 ttgaaggtac tttttcaagg tcttccaagt gaagatatag atacccattg atgctaaatt
     6001 gctcttagga ttttctggtt tttcttcaaa ttcttcaata cggtaatctt cgtccgtatt
     6061 catgatacca aaacgtgaag cttctttgat tggaacttcg ataaccgcaa tcgtggcatc
     6121 tgcttttttc tgaatatgtg tatcaaggag tttgtcgtag ttcatcttat agatgtggtc
     6181 accagacaaa atcaatacgt gctctggatc gtgaagatca atgaaatcaa tgttctgtgt
     6241 aatagcgtcg gctgtacctt tgtaaagtcc gaaaccttcg attttttcac tcggtggtaa
     6301 tacaaagacg ccagatcctt gtacatcaag tccccaacgt tgagactgtg caacgtatga
     6361 gttcaagaga acaggctcat actgagtcaa aactccgaca atatcaatac ctgagttggc
     6421 acagttactg agaggaaagt caataatgcg gtactttccg ccaaatgcga ccgctggttt
     6481 ggcgactttt tttgtcaacc cttctagacg tgttccacgt ccaccagcaa gaatcatagc
     6541 taacatttta ttttgagc
//
LOCUS       DBNFCK010000102         6550 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.102, whole genome shotgun sequence.
ACCESSION   DBNFCK010000102 DBNFCK010000000
VERSION     DBNFCK010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6550
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.102"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..692)
                     /locus_tag="AC1PIW_001560"
     CDS             complement(<1..692)
                     /locus_tag="AC1PIW_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HIP6606470.1"
                     /translation="MSFSVISSYLIQHPVLTALLIAHFLSDFTFQSQALADAKKLHLK
                     ALFMHLFIVAIPLLILALLTPIQNGDLFFQVWLSHLAIDYVKYFLNKHHWIKDSWEAG
                     AFLVDQLLHIISIIILYHTIGVNVASHSLWIEPNYLLLQILFILLISKPVNILFKLYF
                     SKYQVAEGEEEQTVTGAGALIGQLERLIMGIFLLLGQYTAIGLVFTAKSIARYDKISK
                     SQAFAEYYLIGSL"
     gene            complement(696..1364)
                     /locus_tag="AC1PIW_001561"
     CDS             complement(696..1364)
                     /locus_tag="AC1PIW_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HIP6606471.1"
                     /translation="MNYIAIIGDIVNSKQTSNRSSIQERLKQQLNRINHSFAQDFASP
                     FTITKGDEFQALCKPNPYIFFIIDQIQLAFRDEVEIRFGIGLGEILTAIDPKLSIGAD
                     GPAYWEARKAIDFIHDNHDYGTSKIAFSSEYKQIDRVINPLLTSSDFIKAAWNRSQTD
                     LFETLISKKIYQEDFTQKPIAEKMKLSQSAFTKRLKSSGIKLYLRNRQTAMNLILQLS
                     QKEG"
     gene            complement(1558..3096)
                     /gene="ffh"
                     /locus_tag="AC1PIW_001562"
     CDS             complement(1558..3096)
                     /gene="ffh"
                     /locus_tag="AC1PIW_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIP6606472.1"
                     /translation="MAFESLTERLQNVFKNLRRKGKISESDIQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAIGHEVIETLNPAQQIIKIVDEELTAVLGSETSEIIKSPKIPT
                     IIMMAGLQGAGKTTFTGKLANKLKQEENARPLLIAADIYRPAAIDQLKTLGQQIDVPV
                     FELGNQVPALEIVRQGLEQAKANHNDYVLIDTAGRLQIDQALMTELREIKEFAQPNEI
                     LLVVDAMIGQEAANVAREFNDQLAITGVILTKIDGDTRGGAALSVRHITGQPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASKEYDEKKSLELAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMAGNPALANLKVDEREIARKRAIVSSMTPAERENPD
                     LLTPSRRRRIANGSGNSFVEVNKFIKDFNQAKTMMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPDMNGMDMSALEDMMGGAGMPDMSQMFGGGLKGKIGEFAMKQAMKRQANKIKKAK
                     KKRK"
     gene            complement(3109..3441)
                     /locus_tag="AC1PIW_001563"
     CDS             complement(3109..3441)
                     /locus_tag="AC1PIW_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HIP6606473.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FQVSRQAVYDNIKRTEKLLEDYEMKLHMYSDYVVRSQIFDEILNKYPEDAYLKEKIAI
                     LTSIDNRE"
     gene            complement(3544..4242)
                     /locus_tag="AC1PIW_001564"
     CDS             complement(3544..4242)
                     /locus_tag="AC1PIW_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIP6606474.1"
                     /translation="MKAAYITIHDKIKEQIDNGIWKIGQRLPSERDLADEFGVSRMTL
                     RQGITLLVEEGILQRKIGSGTYVASTRVQEKMRGTTSFTELVQLQGKTPSSKLLSYTR
                     TKPNEKEVEQLGLSRGEYVIRMERVRYADNVPVVYEVASIPERLIKNVPKEDVTNHFF
                     KTLMDNGYRIGTSKQTIFARLANEKVAQYLQISKNQAILALKQVSYLEDGQAFEFVNS
                     QYVGERFEFYLENN"
     gene            4518..6068
                     /gene="guaA"
                     /locus_tag="AC1PIW_001565"
     CDS             4518..6068
                     /gene="guaA"
                     /locus_tag="AC1PIW_001565"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIP6606475.1"
                     /translation="MTKQDVQKIIVLDYGSQYNQLISRRIREFGVFSELKNHKITAEE
                     VRAINPIGIVLSGGPNSVYAENAFDIDPEIFELGIPILGICYGMQLITHKLGGKVVPA
                     GEAGNREYGQSNLQLKTESALFAGTPEEQLVLMSHGDAVTEIPADFHLVGLSADCPYA
                     AIENTERRIYGIQFHPEVRHSVYGNDILKNFAFGICGAKGDWTMENFIETEIEKIRQT
                     VGDKKVLLGLSGGVDSSVVGVLLQRAIGDQLTCIFVDHGLLRKNEGDQVMEMLGGKFG
                     LNIIRVDAAKRFLDLLAGVSDPEKKRKIIGNEFVYVFDDEASKLTDVEFLAQGTLYTD
                     IIESGTDTAETIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRALGTALGMPDEVVWRQ
                     PFPGPGLAIRVMGEITEEKLQTVRESDAILREEIAKAGLDRDVWQYFTVNTGVRSVGV
                     MGDGRTYDYTIAIRAITSVDGMTADFAKLPWDVLQKISVRIVNEVDHVNRIVYDITSK
                     PPATVEWE"
     gene            complement(6243..>6550)
                     /locus_tag="AC1PIW_001566"
     CDS             complement(6243..>6550)
                     /locus_tag="AC1PIW_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922381.1"
                     /note="McrC protein together with McrB forms the McrBC
                     restriction system; recognizes N4- and C5-methylcytosine
                     (and 5-hydroxy-methylcytosines); appears to act against
                     5-methylcytosine preceded by a purine residue; MrcC
                     modulates the specificty of McrB and has DNA cleavage
                     activity; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-methylcytosine-specific restriction
                     endonuclease system specificity protein McrC"
                     /protein_id="HIP6606476.1"
                     /translation="KYYSSNTQIRFDKRTLHSNNLYQIFTYVKNQAYRLSDSNDTVAG
                     MLLYAKTDIDIQPNQVYQMHGNRISVKNLDLNLPFASIAEQLDDIITSHFASPPKLY"
BASE COUNT         1870 a         1280 c         1329 g         2071 t
ORIGIN      
        1 agcgaaccaa ttaaatagta ttcagcaaag gcttggcttt tggatatttt atcatagcgt
       61 gcgatggact ttgctgtaaa taccagtccg atagctgtgt attgccccag aagcagaaaa
      121 attcccatta tgaggcgctc caattgacca atcaaggctc ctgcacctgt cacagtctgc
      181 tcttcttctc cttccgctac ctgatatttg ctaaagtaga gtttgaaaag aatattgacg
      241 ggcttgctga tcagaaggat aaaaaggatt tgcaggagga gataatttgg ttcaatccag
      301 agtgagtgtg aagcgacatt aacaccgata gtgtgataaa gaatgatgat tgagatgata
      361 tggagcaatt gatcaactag gaatgctcca gcttcccagg agtcctttat ccaatggtgt
      421 ttgttgagaa aatatttgac atagtcaatc gcaagatggc tcagccaaac ttgaaaaaat
      481 aaatctccgt tttgaatagg ggtaagtaag gctaggataa gaagagggat agcaacaatg
      541 aaaaggtgca tgaaaagagc tttcaaatga agctttttag catccgccag tgcttgactc
      601 tggaaggtaa aatcacttaa aaagtgggca ataagcaagg cggttaaaac tggatgttga
      661 ataagatagc tagaaataac tgaaaatgac atgctttatc cttctttctg actgagctgg
      721 agaatcaaat tcatggcagt ttgacgatta cgcaggtaga gtttgattcc gctggatttc
      781 aaacgtttgg taaaggcgct ttgactgagt ttcattttct cagcaattgg cttttgtgtg
      841 aaatcttctt gatagatttt tttggagatt aaggtctcaa acagatccgt ttgcgaacga
      901 ttccaggcag ctttgatgaa atcgctagaa gttagcaagg ggttaatcac acgatcgatt
      961 tgcttgtatt cagatgaaaa tgcaattttg ctggtgccat agtcatggtt gtcatgaatg
     1021 aagtcaatag ccttgcgtgc ttcccaataa gctggaccat ctgctccaat acttagcttg
     1081 ggatcgatag ctgttagaat ctcacccaaa ccaattccaa atcgaatctc cacctcatca
     1141 cgaaaggcta gttggatttg gtcaattata aaaaaaatat aagggttagg cttgcataag
     1201 gcttgaaatt catctccctt tgtaatggta aatggcgatg cgaagtcctg agcgaaggaa
     1261 tgattgattc tatttagctg ctgttttagc cgctcttgaa tactagagcg gtttgaagtc
     1321 tgttttgaat tgacaatatc tcctattatt gcgatataat tcataactta tcctctaatt
     1381 tctattatat ttccattata acggacaata taaagattgt ccactaaaaa ggacattttt
     1441 gaatatttct gttataacgg aaagtgttgg agtttgaaaa tcatttttcc tgctttgcta
     1501 cttctatttt atattgctat atcttacttc ttcgaataaa aagtaaaaaa gaagagatta
     1561 ttttctcttc tttttggctt tcttgatttt attggcttgg cgtttcatgg cttgtttcat
     1621 agcaaactca ccaattttac ctttcaaacc gcctccaaac atttgggaca tatctggcat
     1681 accagctcct cccatcatat cttccagagc agacatatcc ataccgttca tgtctggcat
     1741 gtttttaggg agattatttg ggttgattcc catttgtttc atcattttgt tcatgtcgcc
     1801 tgacatgaca ccctgcatca tggttttcgc ttggttgaaa tccttgataa atttattgac
     1861 ttcgacaaag ctatttccag aaccgttagc aatacgacga cgacggcttg gtgtcaacaa
     1921 atcaggattt tctcgttcgg ctggtgtcat agaagatacg atagctcgtt ttcgtgcaat
     1981 ttcacgctcg tcaactttca agttggcaag tgctgggtta ccagccatac ctggaatcat
     2041 cttcagcagg tcttccattg gtcccatatt ttgaacttgg tccagctgat caatgaaatc
     2101 gttgaaatca aaggtattct cacgcatttt ttcagcgagt tctagtgatt tcttctcatc
     2161 atattctttg ctggcttttt caatcaaggt cagcatgtcg cccatgccca aaatacgcga
     2221 ggacatacgg tctgggtgga aggtttcaat atctgtgatt ttttcaccag taccggtgaa
     2281 tttgattggc tgaccagtaa tgtgacggac agacaaggct gcaccaccac gtgtatcgcc
     2341 gtcaatcttg gtcaaaatca caccagtaat agccagttga tcattgaact cacgcgcaac
     2401 gttggcagct tcctgaccaa tcatagcatc gacgaccaag aggatttcgt ttggctgagc
     2461 aaactctttg atttctctta gttcagtcat gagggcttgg tcaatctgca aacgacctgc
     2521 tgtatcgatc agaacgtagt cattatgatt agccttggct tgctccaaac cttggcgtac
     2581 aatctccagg gcagggactt gattgcctaa ctcgaataca ggtacatcaa tttgttgacc
     2641 aagggtcttc aactggtcaa tggcagctgg acgataaata tcggcagcaa tcaagagtgg
     2701 acgggcattt tcttcctgct tgagtttatt agccaactta cctgtgaagg tcgttttacc
     2761 agcaccctgc aatccagcca tcataatgat agttggaatt tttggtgatt tgataatttc
     2821 tgaagtttca gaacctaata cagccgtcaa ttcttcatca acgattttga taatttgttg
     2881 agcggggttc agggtttcaa tgacttcatg accgatagca cgttcacgca ccttcttgat
     2941 aaagtctttt acaactggca aggcaacgtc ggcttctagt aaggccagac gaatttcctt
     3001 agttgcttcc tgaatatcac tctcagaaat ttttccctta cgacgcagat ttttaaaaac
     3061 attctgtaaa cgttctgtta agctttcaaa agccatatac ttctcctctt attctctatt
     3121 atcaatactg gttagaatag cgattttttc cttcaaataa gcatcttctg gatacttgtt
     3181 cagtatttca tcgaaaatct ggctacgcac tacatagtca gaatacatat gtagtttcat
     3241 ctcgtagtct tccagtaatt tttctgttcg tttaatgtta tcgtagaccg cctgacggct
     3301 gacttgaaat tcttcagcaa tctcagccaa actgtaatca tcggcataat agagctcgat
     3361 gtagttcatt tgcttgtctg ttagcaaggc tgcatagaat tcaaataagg cattcattcg
     3421 gttggttttt tcaatttcca taaacttcat tatatcaaaa attccatatt ttataaataa
     3481 gaaaatccgt catcactaga aagattacgg attttaaaaa tgttttacat aattattttt
     3541 atgttaattg ttttctaaat agaattcgaa acgttcccca acatactgac tatttacaaa
     3601 ttcaaatgct tgaccatcct cgagataaga tacctgtttt agtgcgagaa tagcctggtt
     3661 tttagaaatt tgtaaatatt gagccacttt ttcattggct aaacgcgcaa aaatagtttg
     3721 tttgctcgtt ccaattcgat aaccattgtc cataagtgtt ttaaagaaat ggttggtaac
     3781 atcttctttt gggacatttt ttatcaagcg ttcaggaatg ctggcaacct catacacgac
     3841 gggaacatta tctgcatagc ggacccgctc cattcgaata acatactcgc cacgggataa
     3901 acctaattgt tcaacttctt tttcattagg tttggttcgt gtataggaca ataatttgct
     3961 gcttggcgtc tttccttgga gttgtactaa ttcagtaaag gaagtcgtgc ctcgcatctt
     4021 ttcttgaacc cgtgtactgg cgacataagt accggaacca atcttgcgtt gtagaatacc
     4081 ttcttccact aaaagtgtga ttccttgacg gagcgtcata cgagaaacac caaattcatc
     4141 tgctaaatcg cgttcacttg gtaaacgttg accaattttc cagattccgt tatcgatttg
     4201 ttccttaatt ttatcgtgaa ttgtgatata tgctgctttc atttatctag cctttctctt
     4261 ctttctagtt taccaaaaat aaaggcgttt ggctaattca ggccacactc tattggtaaa
     4321 attctcctag gcaaacaatg aagtcagatt acctaaaata aagtgagtaa aaacaataaa
     4381 aatacgaaca ctaacatttt tattatcact catagtttgc aaaacagata tttaacaaat
     4441 aaaatttgga aaatgttcgc ttttatgtta aactaactac tagtatttga atttttttca
     4501 aaacgaaagg gaaaaacatg acaaaacaag atgtccaaaa aattattgta ttggattatg
     4561 gtagtcagta caaccaactt atttcacgtc gaattcgtga atttggtgtc ttttcagaat
     4621 tgaaaaacca taaaattaca gctgaggaag tccgtgctat caatccaatt ggtatcgttc
     4681 tttcaggtgg accaaactct gtctacgcag aaaatgcctt tgatattgat ccagaaatct
     4741 ttgaattggg tattcctatc ttagggattt gctatggtat gcagttaatt acccataaat
     4801 tgggaggtaa ggttgttcct gctggtgagg ctggaaatcg tgaatatggt caatcaaatc
     4861 ttcaactgaa gactgaatca gcgctttttg ctggaacacc agaagaacag cttgtactca
     4921 tgagccacgg ggatgctgta actgaaattc ctgctgattt ccacctggtt ggtctttctg
     4981 ctgactgccc ttacgcagcc attgaaaaca cggaacgccg tatctacggt atccagttcc
     5041 acccagaagt tcgtcattcc gtatatggaa acgatatttt gaaaaacttt gcgtttggta
     5101 tctgtggagc caaaggcgac tggacaatgg aaaacttcat cgaaacagaa attgaaaaaa
     5161 tccgccagac tgttggggac aagaaagtct tgctcggttt gtcaggcggt gtagattcgt
     5221 ctgttgttgg ggtccttctt caacgtgcta ttggtgacca attgacctgt atcttcgttg
     5281 accacggtct tcttcgtaag aatgaaggtg atcaggttat ggagatgttg ggcggtaaat
     5341 ttggcctcaa tattattcgc gtcgatgctg ctaagcgttt cttagaccta cttgctggcg
     5401 tatctgaccc tgagaagaaa cgtaaaatca tcggtaatga atttgtttac gtatttgacg
     5461 atgaagcgag caagttgact gatgttgaat tcctagcgca aggaacactt tataccgata
     5521 ttatcgagtc aggaacggat actgctgaga caatcaaatc tcaccacaac gttggtggtc
     5581 tgccagaaga tatgcagttc aagttgattg aaccgctcaa tactctcttt aaggatgaag
     5641 ttcgtgccct cggtactgct cttggtatgc cggatgaagt tgtatggcgc caaccattcc
     5701 caggaccagg ccttgccatt cgtgtcatgg gtgaaatcac tgaggaaaaa cttcaaactg
     5761 ttcgcgaatc tgacgctatc ttgcgtgagg aaattgcaaa agcaggtctt gaccgcgatg
     5821 tgtggcaata tttcacggtt aatactggcg ttcgctccgt aggtgtcatg ggagatggtc
     5881 gtacctacga ctacactatc gctatccgtg ctatcacttc tgtcgatgga atgacagctg
     5941 actttgccaa actaccatgg gatgttcttc aaaaaatctc tgtccgtatc gtaaacgaag
     6001 tcgaccacgt caaccgcatc gtctatgata taacaagtaa accaccagca acagttgagt
     6061 gggagtaaat tagtccagtg gactaattta cccgatgcct aaaaacaaaa aagtgtcgct
     6121 gatctgggaa atcaattaac cagacgctcc aaaataggaa agagtcttat aaaaaaacaa
     6181 tcaaagagta gtttagtgct actactttga ttgttttttt gttttctagc ttagagctct
     6241 aactagtaca gttttggagg ggatgcaaaa tgcgatgtaa tgatgtcatc cagttgttca
     6301 gcaatacttg caaacggcag gtttaagtcc aaatttttga cactaatccg gtttccatgc
     6361 atttggtaga cctgattagg ttgaatatct atatcggttt tagcgtatag aagcattcct
     6421 gctactgtat cgtttgaatc tgacaggcga taagcctggt ttttcacata ggtaaaaatt
     6481 tggtatagat tatttgaatg gagtgtgcgc ttatcaaagc gaatctgtgt gttactagaa
     6541 taatatttag
//
LOCUS       DBNFCK010000103         6536 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.103, whole genome shotgun sequence.
ACCESSION   DBNFCK010000103 DBNFCK010000000
VERSION     DBNFCK010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6536
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.103"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(92..622)
                     /locus_tag="AC1PIW_001567"
     CDS             complement(92..622)
                     /locus_tag="AC1PIW_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIP6606477.1"
                     /translation="MKQNQLRQLTEISLFAALICVVVFFIRIPFGSQFVHLGNALVVV
                     GVLIFGAKKGALAASLGLGIFDLLSGYASVAWITILESLIVCFVLHLFYEKFLQANDS
                     LSNIMLVGIVAALTKIILNLLKYTLTGSLVGGLTPSVLFLAALGRILGTYGTAITTII
                     AVPILYQVCKRLYKRK"
     gene            complement(619..1464)
                     /locus_tag="AC1PIW_001568"
     CDS             complement(619..1464)
                     /locus_tag="AC1PIW_001568"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HIP6606478.1"
                     /translation="MQRLLLANDLPGVGKVALATSIPIAAACQVETMLLPTVLLSSHT
                     GGFPDVVIEDMTDLNQAYFEQWKNLNLELAGILSGYCRNPQQLSQLAMYAKQTETPLI
                     VDPIMGDSGRLYTGFTSSYVEAMKELTQSAQLILPNITEASLLTGRNYLGDSYDIHDI
                     EDLLEALAEQVSADIVLTGISFDDSQIGVAYFQQETGEITTYMSKKYPANFFGTGDIL
                     STLLAVATIQQINLHQAIPLALDFIDKSLERTLALDRDLKFGISFEPFLAELQQAFQI
                     LKEKL"
     gene            complement(1663..2352)
                     /locus_tag="AC1PIW_001569"
     CDS             complement(1663..2352)
                     /locus_tag="AC1PIW_001569"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HIP6606479.1"
                     /translation="MKFGIIAAMPQELKILVEHLQDATEIDVLGRTYYQGRIGQHEVV
                     LVQSGIGKVMSAMSVAVLADRFSVDVIVNTGSAGAVAEGIAIGDVVVANQLAYHDVDV
                     TAFGYAYGQMAGQELYYPADQVLLEQLRTVLAEQEMISHVGLIVTGDSFIAGQERIAT
                     IKTHFPEVLAVEMEGAAIAQAAVNTGKPFLVIRAMSDTAQGDANITFDEFIIQAGERS
                     AQTLIAFLEKY"
     gene            complement(2361..2681)
                     /gene="macP"
                     /locus_tag="AC1PIW_001570"
     CDS             complement(2361..2681)
                     /gene="macP"
                     /locus_tag="AC1PIW_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HIP6606480.1"
                     /translation="MGKPLLTDEILEKVKKKQYSDQSSQFGHYRPNQDISMDDALFDP
                     YLEEDYQGYEEGQTIRIPVKPTVVKSRRIETIKREAFRSKVNKILFWVIILLIMFIIA
                     VLFI"
     gene            complement(2692..3237)
                     /locus_tag="AC1PIW_001571"
     CDS             complement(2692..3237)
                     /locus_tag="AC1PIW_001571"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIP6606481.1"
                     /translation="MNFEEKTIERTEIFKGHIFDVVVDDVALPNGGTGKRELIFHKGA
                     VCVLAVTPEGKMILVKQYRKAIERTIYEIPAGKLELGEEDTLEDAALRELEEETGYTS
                     DKLTLLADFYSAIGFCNERIRLYLADNLIKVENPRPMDEDEVIELHEVTLEEALNLVA
                     TGDICDAKTIMAIQYLQLMRK"
     gene            complement(3246..4628)
                     /gene="glmU"
                     /locus_tag="AC1PIW_001572"
     CDS             complement(3246..4628)
                     /gene="glmU"
                     /locus_tag="AC1PIW_001572"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HIP6606482.1"
                     /translation="MSNNYAIILAAGKGTRMKSDLPKVLHKVAGITMLEHVKRAVDAM
                     EPAKTVTIVGHKAELVQAVLEGQSEFALQSEQLGTGHAVMMAEPALAGLEGQTLVIAG
                     DTPLITGESLKNLINFHVSHKNVATILTAQADNPFGYGRIIRNADGEVQKIVEQKDAN
                     DFEKQVKEINTGTYLFDNKRLFEALKDINTDNAQGEYYLTDVISIFRQAGEKVGAYVL
                     RDFDESLGVNDRVALATAEAVMRKRINEKHMVNGVTFINPDATYIDIDVEIGAEAVIE
                     ANVVLKGQTVIGERTVLTNGTRVRDAKIAADAVISNSDIEESVIEEGVTVGPYAHIRP
                     GSLLKKDVHVGNFVEIKASTLGQGTKSGHLTYLGNATIGNNVNVGAGTITVNYDGKNK
                     FKTTVGDNAFVGSNSTIIAPVTIGDNALLAAGSVITKDIPEDAIGIGRGRQENKEGYA
                     TRFPFHPSQK"
     gene            complement(4804..5274)
                     /locus_tag="AC1PIW_001573"
     CDS             complement(4804..5274)
                     /locus_tag="AC1PIW_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HIP6606483.1"
                     /translation="MTPTQLWQEFIAINSQAGPEPEAWAFGAEADRLADLVARGIKTS
                     TSSAHALYEVEGEEIPRAGGYDIILDGQGQAVCIIQTTKVYVTPFSQVTAEHAYKEGE
                     FRQGGDLSDIKAKSLIHWRQIHEELFTIWLAEAGLSFSEDMLVVCEEFELVYPK"
     gene            complement(5271..5447)
                     /locus_tag="AC1PIW_001574"
     CDS             complement(5271..5447)
                     /locus_tag="AC1PIW_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606484.1"
                     /translation="MWQFLCGDIHDIADVQLIALVEILQFGITETLLNQIPYGFEALL
                     DQAKNWQISRKDPL"
     gene            complement(5563..6261)
                     /locus_tag="AC1PIW_001575"
     CDS             complement(5563..6261)
                     /locus_tag="AC1PIW_001575"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HIP6606485.1"
                     /translation="MTKTALITGVSSGIGLAQAGIFLENGWRVFGIDLASKPDLAGDF
                     HFLQLDLTGDLSPVFSWCQSVDVLCNTAGILDDYRPHLDISEDELAQIFAVNFFAVTR
                     LTRPYLQQMVDRQSGIIINMCSIASSLAGGGGSAYTASKHALAGFTKQLALDYAKDKV
                     QIFGIAPGAVQTGMTQKDFEPGGLADWVADQTPIGRWTQPSEIAELTFMLATGKLASM
                     QGQIITIDGGWSLK"
     gene            complement(6254..6490)
                     /locus_tag="AC1PIW_001576"
     CDS             complement(6254..6490)
                     /locus_tag="AC1PIW_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018367459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HIP6606486.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGGAENYVQLFDTIEVHGQKLEAT
                     PYFLINVTGEGEGFSMWSPTPCDVLATDWVEVHD"
BASE COUNT         1832 a         1540 c         1275 g         1889 t
ORIGIN      
        1 agagtataac ctcctgtttg tttcatcact tacagtgtag tccttctgac cttttttgca
       61 tattgaattt aactaatgtc accaatataa attattttct cttatataac cttttacata
      121 cctgataaag aataggtaca gcaatgatgg tcgtaattgc agtgccatag gttcctaaaa
      181 tcctacctaa agcagctaaa aataatactg atggcgttag tccaccgact aaactacccg
      241 tcaatgtgta cttcaacagg ttcaagataa tttttgtgag ggctgcaact atcccaacca
      301 acataatatt tgatagacta tcgtttgcct gtaggaattt ctcataaaat aagtgtagga
      361 caaaacagac aatgagtgat tctagaatgg taatccaagc aacggaggca tatcccgaca
      421 gtaaatcaaa gattcctaga ccgagtgagg ctgccagcgc cccttttttg gcaccaaaga
      481 ttagtactcc tactaccacc agagcattgc ctagatggac aaattgactc ccaaaaggaa
      541 tacgaataaa gaagacaacc acacaaataa gggctgcaaa taggctaatt tctgttaatt
      601 gacgcaattg attctgtttc ataatttctc ctttaatatt tgaaatgcct gctgtaattc
      661 tgctagaaaa ggttcaaagg aaatcccaaa tttcaagtct ctatccaaag caagggtacg
      721 ctctaaactc ttatcgataa aatctaaagc tagagggata gcttgatgga gattgatttg
      781 ttggatggtt gctacagcca acaaggtaga tagaatgtca cctgttccaa agaaatttgc
      841 tggatatttt ttagacatat aggtagttat ttcccctgtt tcctgctgaa aataggctac
      901 acctatttgg ctatcatcaa aggaaatccc tgtcagtaca atatctgcgg atacctgctc
      961 tgctaaagct tccagtaagt cctcaatatc atgaatatcg taggaatctc ccaaatagtt
     1021 tctcccagtc aatagagaag cttctgttat attcggaaga atgagctggg ccgactgagt
     1081 caattctttc atggcctcta cataggatga tgtaaaacct gtatacagtc ttccactgtc
     1141 acccataatc ggatctacaa tcaatggtgt ctctgtttgt ttagcataca tagccaattg
     1201 acttagttgc tgaggatttc tacagtagcc agacaagatg cctgccaact ccaaattcaa
     1261 gttcttccac tgttcaaaat aggcttgatt caagtcagtc atatcttcaa tgacaacatc
     1321 tgggaaaccg cctgtgtgtg aggataaaag aactgtaggt aaaagcattg tttctacctg
     1381 acaagctgct gcaattggga tgctagttgc tagtgcaacc ttaccaacac caggtaagtc
     1441 gttcgcaagc aataatcgct gcatgataac tacctcctaa attatttttc ataaacaaaa
     1501 ttgtaaagga ttatcataca tttttcaatc ttacatatcc gcaactgtat ggggacagat
     1561 agctgaaacc ttgcaacacc aggctcaaat tattacataa gaaaaacagc aacaactctt
     1621 tgctactgtc atcctcttca atgtattgtt gtcaaattct gtttaatatt tctccagaaa
     1681 ggcaatcagg gtctgggcag aacgttctcc tgcttgaatg ataaattcat caaaggtgat
     1741 attggcatct ccctgagctg tgtcactcat ggcacggata accaagaatg gcttacctgt
     1801 attgacagct gcttgagcga tagctgctcc ttccatctct acggcaagga cttctgggaa
     1861 atgagttttg attgtagcga ttctctcctg acctgcgata aaactgtcac ctgtcacaat
     1921 cagacccaca tggctaatca tttcctgctc tgctaaaacg gttcttagct gttctaacaa
     1981 gacttggtcg gctggataat acaattcctg ccctgccatt tgcccgtaag catagccgaa
     2041 agccgtcaca tccacatcgt ggtaagccaa ttgattcgct acaaccacat caccgatggc
     2101 aataccttca gcaacagctc ctgccgaacc tgtattgaca atgacatcaa ccgaaaatcg
     2161 atctgccaaa acagccacgg acatagctga cataacctta ccgatacctg actgaacaag
     2221 aacgacttca tgttgaccga ttcgtccctg atagtaagtt cgacccaaga catcgatttc
     2281 agttgcatct tgtagatgct cgaccaaaat ctttaattcc tgaggcatgg ctgcgataat
     2341 tccaaatttc atctcttctc ctaaataaat aatactgcaa tgataaacat tatcagtaag
     2401 atgatgaccc aaaaaaggat tttgtttacc ttactgcgga aagcttctcg cttgatggtt
     2461 tcgatacggc gactttttac tacagtaggt ttgaccggga ttcggatcgt ttgtccctct
     2521 tcatatccct gataatcttc ttctagatat ggatcgaata gagcatcatc cattgatata
     2581 tcctggtttg gacgatagtg tccaaactgg ctagattggt cggaatactg tttctttttc
     2641 actttctcta aaatttcatc cgtcaacaag ggcttgccca ttgctagttt cctactttct
     2701 catgagttgt aaatattgga tagccatgat ggtctttgca tcacaaatat cgccagtagc
     2761 caccaagttc aaagcctctt ccaaggttac ctcatgtaat tcgataacct catcttcatc
     2821 cattggacga ggattttcaa ccttgataag gttatctgct aaataaaggc gaattcgttc
     2881 attacagaaa ccgatggctg aatagaaatc tgccaacaag gtcaatttgt cactagtata
     2941 gcctgtttct tcttctaatt cacgcaaagc agcatcttca agcgtatctt cttcgcccag
     3001 ctccaatttc cccgccggaa tttcataaat agtacgttca atggccttgc ggtattgttt
     3061 gaccaaaatc attttacctt ctggggtaac ggctaaaacg caaactgctc ccttgtggaa
     3121 aatgagttcg cgcttaccag ttccaccatt tggcaaggca acatcatcta ctactacatc
     3181 aaaaatgtgc cctttaaaaa tttctgtccg ctcaatggtt ttttcttcaa aattcatatg
     3241 attacctatt tttgacttgg atggaacgga aaacgagtcg cataaccttc tttgttttct
     3301 tgacgaccac gaccaatacc gatggcatcc tctggaatgt cttttgtaat gacagaacct
     3361 gcagccaaca aggcgttgtc cccaatagtt actggtgcga tgatggttga attagagcca
     3421 acaaaggcat tatctccaac ggttgtttta aatttatttt taccgtcgta gttgacagta
     3481 atggtaccag caccaacgtt aacattattg ccaatcgttg catttcctag gtaggtcaaa
     3541 tgaccagact tcgttccttg accaagtgta gaggccttga tttccacaaa gtttccaacg
     3601 tgaacatctt ttttcaagag actacctgga cgaatatggg cgtatggtcc taccgtcaca
     3661 ccttcttcaa taacagattc ttcaatgtct gagttgctga tgactgcatc tgctgcaatt
     3721 ttggcatcac gtacacgagt accattggtt aaaacagtac gttcaccaat caccgtttgc
     3781 cctttcaaga caacattagc ttcgataaca gcttctgcac caatctcaac atcaatatca
     3841 atataggttg catctgggtt gataaatgtc acaccattga ccatgtgttt ttcattgatt
     3901 cgtttgcgca taacagcttc tgcagtcgca agagcgacgc gatcattgac acccaaactt
     3961 tcatcaaaat cacgcaatac ataggcacca accttctcgc ctgcctgacg gaaaatcgaa
     4021 ataacatctg tcagatagta ctcaccttgg gcattgtccg tattaatatc tttaagcgct
     4081 tcaaaaagac gtttattatc aaacaagtag gttcctgtat tgatttcttt cacttgtttt
     4141 tcaaaatcgt tggcatcttt ttgctcaaca attttttgca cttcaccatc agcattgcga
     4201 ataatccgac catatccgaa tgggttatca gcctgagctg tcaagatagt cgcaacattc
     4261 ttgtggctga catggaagtt aataaggttc ttcaagcttt ctcctgtaat cagcggtgta
     4321 tcccctgcga taacaagggt ctgaccttcc aaacctgcaa gagctggctc agccatcata
     4381 acggcgtggc ctgtccccaa ctgttcagat tgaagggcaa actctgactg accttcgaga
     4441 actgcttgga ccaattctgc cttgtgaccg acaatcgtta ctgttttagc aggttccata
     4501 gcatctacag cccgtttgac atgctccaac atggtaattc ctgccacttt gtgtaagacc
     4561 tttggcaagt cagacttcat gcgagttccc ttacccgccg ctaggataat tgcatagtta
     4621 ttgctcatag ttttctctct tttcaatgat ataccctatt atagcaaatt ttgatgtttt
     4681 tggaaaatag caatattata gggaattttc gtaaataaaa aagaaactgg aatgaagcca
     4741 gtttctcaaa aatttctagt attagaaaac aaactacagg ttgattgcat cctatagaat
     4801 tgtctattta ggataaacca attcaaattc ttcacagacg accagcatat cttctgagaa
     4861 ggacaggcct gcttctgcca gccagatagt gaaaagctct tcatggatct gtcgccagtg
     4921 gatcagactt ttcgctttta tgtcagacaa gtcacctccc tgacggaact cgccctcctt
     4981 gtaggcatgc tctgctgtca cttgggaaaa gggcgtcaca tagaccttgg tggtctggat
     5041 aatgcaaaca gcctgacctt gtccgtccag aatgatgtca tagccaccag ctcttgggat
     5101 ttcttctccc tctacttcat agagagcatg ggcagagctg gttgaagttt tgatgcctct
     5161 agccaccaaa tcagccagtc gatcagcttc cgccccaaag gcccaagcct ccggctcagg
     5221 acctgcttgg gaattgatgg caatgaattc ttgccagagt tgtgtcgggg tcataggggg
     5281 tcctttctag aaatttgcca attcttcgct tgatcaagta aagcctcaaa tccataaggg
     5341 atttgattta gaagtgtttc ggttatccca aactgaagta tctcaactaa agcaattaat
     5401 tgaacatctg caatgtcatg aatatctcca caaagaaatt gccacatccc atcttccaag
     5461 tcatgactga tgtaggttat cggtttattc tcttcactat gtgtttacag atcactactg
     5521 ctacatgagg attatcatca aaaatcaaca tcactttcct ccctacttca aactccagcc
     5581 accgtcaatg gtgataatct ggccttgcat ggaagcgagt tttccagtag ccagcatgaa
     5641 ggtcagctct gcgatttcgc ttggttgggt ccagcgtccg ataggggtct ggtctgccac
     5701 ccagtccgcc agaccaccag gctcaaagtc cttctgggtc atgcctgtct ggacggcacc
     5761 aggagcgatg ccgaagatct ggaccttgtc cttggcatag tccagtgcca actgcttggt
     5821 aaaacctgcc aaagcgtgct tggaagcggt ataggcagag ccacctccac ctgctagact
     5881 ggaggcaatg gagcacatat tgataatgat gccagactgt ctgtccacca tttgctgcag
     5941 ataggggcga gttagtctag tcaccgcaaa aaaattcacc gcgaagatct gagctaattc
     6001 gtcctccgaa atatcgaggt ggggtcggta atcgtctaaa atgcctgcgg tgttacagag
     6061 gacatcgact gactggcacc aggaaaagac gggcgataaa tcgcctgtca ggtccagctg
     6121 tagaaagtgg aaatcgcctg ccaaatcagg cttgctagcc agatcaatcc cgaagacacg
     6181 ccagccattt tccaaaaaaa tccctgcctg tgctagacca atgccgctgg aaacgccggt
     6241 gatgagggct gtcttagtca tggacttcta cccagtcggt cgcaaggaca tcgcaagggg
     6301 tcgggctcca catggagaag ccttcaccct cgccggtcac gttgatgagg aaatagggcg
     6361 tcgcctctag cttctgcccg tggacctcga tggtgtcaaa gagctggacg tagttctccg
     6421 ccccacccca gcctgtgcga acgtactttt tcttggcctt caagcctggt aaaatctctt
     6481 caaatgtcat gattattcct tttcttattt attttaacac aaatgttttc agtaac
//
LOCUS       DBNFCK010000104         6419 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.104, whole genome shotgun sequence.
ACCESSION   DBNFCK010000104 DBNFCK010000000
VERSION     DBNFCK010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6419
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.104"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..282
                     /locus_tag="AC1PIW_001577"
     CDS             <1..282
                     /locus_tag="AC1PIW_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIP6606487.1"
                     /translation="LVLAIVEKQDSYGYEISQTIKIVADIKESTLYPILKKLEKAGYV
                     TTYTQEFQGRKRKYYTITPAGREHIPFLQGEWNSYKDHIDGIIEGSLRK"
     gene            279..1391
                     /locus_tag="AC1PIW_001578"
     CDS             279..1391
                     /locus_tag="AC1PIW_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIP6606488.1"
                     /translation="MTRADYLNQLEQALFQLHPSARQEALDYFNEYFDEKDNDEEAII
                     ELGTPDEAAKEIIANLPEDALITEQDQASPHSSKPFDFNFDFDFQFDWENFFNNFKHS
                     AYQARERIKLTAKIEELPEFSNLQISLEDQALSVQSYNGEAVLLHNPVSEDGSYQAFD
                     YQLVQDSLYLTSKPTPNHLYFSNNQTSSAILKIPKKLLPLTSLNLKTTDSSLTMAAVQ
                     ASEQLVINAKDSSISMTKVSCPSSALRLEDSTLTISDGQMSELKLEASDAVITLSSMS
                     LSDARITLEDCVWKLKDFVIEKSLNCQAEDSVLTYKPSTDISLDIWEEDSSLKLPKDK
                     AFTMMKEGDITHLSYKQENSPAQLVIHCQDCQLSVG"
     gene            1403..1981
                     /locus_tag="AC1PIW_001579"
     CDS             1403..1981
                     /locus_tag="AC1PIW_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HIP6606489.1"
                     /translation="MNRKEYLQKLEKYLKNLPKEDFQDTLDYFNEYFDEKENDEQAIQ
                     ELGSPKEAAHEILANLYDKEKTEDKPNTRNMVWLTILSILAAPIGVPLAITLVALFIT
                     LLLIIFSLFLLLISLWIVLLSLAIAQLLLAFDLFTLSWSTSLLFTGLALICLALTLLG
                     SKFTLDLGSKTFLLVLHWIQRKIRKGGYYETA"
     gene            1968..2822
                     /locus_tag="AC1PIW_001580"
     CDS             1968..2822
                     /locus_tag="AC1PIW_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIP6606490.1"
                     /translation="MKQLKPFTKISLSLFTLGTILSLIGYLAGGWTNMKTVAENYFQP
                     QTASFTDIRSIEVNEKLSIMPSPDKQFHLHYYRNQHKGIDLLSHQVQDGKLTISSAYQ
                     GIVTYDGLLEPILNLVNDQNVAYYQPVLQVPKNSTIETLSGSIQGDVYLDKVTIQDFQ
                     LFVENGDMLIENSQLSFAHLSHLNGEVAINHSTLISPKDISDTVGSSLTIEDGDFKAE
                     NLKLEGRHSISNYGGSIDISLHSKTKTDIHIDASQNNLGIDLPTYSNPQSTNYLNIST
                     LDGELSIR"
     gene            complement(2859..3218)
                     /locus_tag="AC1PIW_001581"
     CDS             complement(2859..3218)
                     /locus_tag="AC1PIW_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HIP6606491.1"
                     /translation="MSIFTTILASIVAIEHLYIMYLETFATHSDSTSRVFNMEKEELQ
                     RKSVTALFKNQGIYNGLLAVFLFYGIFTGNLEITSIFVLCVIAAAAYGAITASPKIIL
                     TQGGPAILALISILLFK"
     gene            complement(3238..3663)
                     /locus_tag="AC1PIW_001582"
     CDS             complement(3238..3663)
                     /locus_tag="AC1PIW_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIP6606492.1"
                     /translation="MQEMEDLLYRLKVTDETISNLFEKQLGISLTRYSILQTLLKDAP
                     LHQLALQERLQIDRAAVTRHLKLLEESGYIIRKRNPDNQREVLVWPTEQAREALITNP
                     SAHHQAIKASMNQILTVEESEQFLATLDKLLIGLQNLPI"
     gene            3825..4583
                     /locus_tag="AC1PIW_001583"
     CDS             3825..4583
                     /locus_tag="AC1PIW_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HIP6606493.1"
                     /translation="MENTPRLNLVIDVLMLAGAILAQSGAEIHRVEDTMIRIAHSQGI
                     EQANVLAIPAAIFFSIDHTNISRMKRIVNSNYDMQKVCDVNQISRSLANEEISLEEAW
                     SQLNAIKQQKPPYNNIQITLAAVVAAPFFTIMFGGSLLDAAGAVLATALAFPISLIVE
                     RYIGIDFVTTFVGAFVFSLTSYLLSATTTLPINPNLINAGAVMPFVPGIAFTNAVRDL
                     MTNHINTGMTKFFQTALIILSLGAGTTVAFMLVR"
     gene            4587..5039
                     /locus_tag="AC1PIW_001584"
     CDS             4587..5039
                     /locus_tag="AC1PIW_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HIP6606494.1"
                     /translation="MTPVHFLLQALASYIAIAAFLIVLNVQRKMLVPGGLLGMLVWLI
                     YLSLLGPTNVLIATFFAAIIGSCVSQIMSIWLKTPSVIFSLAILAPLVPGYRAYMTTT
                     YFVSGDHAQALTNITTVLTLALVIPIGMASGTILLHLYKVLRTGKKAA"
     gene            5096..6334
                     /locus_tag="AC1PIW_001585"
     CDS             5096..6334
                     /locus_tag="AC1PIW_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIP6606495.1"
                     /translation="MNQNVTNLKLAERGALLSIGAYIVLSGIKLVAGQLFHSDALRAD
                     AFNNISDIIGNIAVLVGLKMAQKPADTDHKFGHWKMEDLASLITSFIMFVVGFQVLYD
                     TLQKLISNSSIEVDIMGAIVGIFSALVMLAVYLYNNRLAKKVRSKALEAAAKDNLSDA
                     VTSIGTSIAIFAAAFNFPIVDKIAAIIITFFILKTAYDIFMESFFTLSDGFDENLLKK
                     YEEDILKLPKIVSVKSQRGRTYGANIYLDVVLEMNPDLSVYESHEVTEQVEQLLTLKH
                     GVFDVDIHVEPSEIPHDEMYEHVYDKLFRFETEIQAHANGYEELIDDQYLLIDAKGRY
                     RNKTQMLADHPIQTTYLSNYQMTSISQKSKLVTFEIGDYVHTSLWRRHENWTVIFHQI
                     SKKQASQIEATEKSSDLHNP"
BASE COUNT         1978 a         1454 c         1152 g         1835 t
ORIGIN      
        1 ctggtcttag ccatcgtgga aaagcaggat tcctacggct atgaaatcag tcaaaccatc
       61 aagatagttg ctgatataaa ggaatccaca ctctacccta ttttgaaaaa attggaaaag
      121 gcgggctacg tcaccaccta tacccaagaa tttcaaggga gaaagagaaa atactacacc
      181 atcacacctg ctggacgtga gcacatcccc ttcctgcaag gtgaatggaa tagctacaaa
      241 gaccatattg acggaattat agaagggagc ttacgaaaat gacacgcgca gattatttga
      301 accaactgga acaagcatta tttcaacttc atccaagtgc cagacaggag gctttagact
      361 acttcaatga atattttgac gaaaaggata acgacgagga agctattata gagctaggaa
      421 cacctgatga agcagcaaag gaaatcattg ccaatctacc tgaagatgcc ctaatcaccg
      481 agcaagacca agcaagtcca cattcatcta aaccatttga tttcaacttt gattttgatt
      541 ttcaatttga ctgggagaat tttttcaaca attttaaaca ttcagcctat caagctaggg
      601 aaagaattaa attgacggcg aagattgaag aattacctga gttttcaaat ctacagattt
      661 cgctcgaaga tcaggctctt agcgttcaaa gttataacgg agaagctgtc ttattgcaca
      721 atcctgtttc agaagatggt agctaccagg cttttgacta tcaactggtt caggatagtt
      781 tgtacctaac aagcaagcct acccctaatc acctctactt ctcaaacaat caaaccagct
      841 ctgctattct caaaatccct aaaaaactac tgccccttac tagtctcaat ctgaaaacga
      901 cagatagtag tctgacgatg gcggctgtgc aagcgagcga acagctagtc atcaatgcaa
      961 aagatagcag tatcagcatg acgaaagtga gctgcccaag ctctgctcta cggttagaag
     1021 actctacact aactatctca gatggacaga tgagcgagtt aaaattggaa gccagcgatg
     1081 ctgttatcac tctctcaagc atgagccttt cagatgcaag gataacatta gaagactgtg
     1141 tctggaagtt gaaggatttt gttattgaaa agagccttaa ctgccaggct gaagattctg
     1201 tcttaaccta taaaccaagt acggacatca gtttagacat ctgggaggaa gattctagcc
     1261 ttaaacttcc aaaggataag gcgtttacaa tgatgaaaga gggtgacatc actcatctca
     1321 gctacaagca agaaaatagc cctgctcagc tagttattca ttgtcaagac tgccagctta
     1381 gtgtgggata aaggaggttt ccatgaatcg caaggaatat ttacagaaac tcgaaaaata
     1441 tctcaaaaac ctaccaaaag aagattttca agataccttg gactatttca atgaatattt
     1501 tgacgaaaag gaaaacgatg agcaagctat tcaagagcta ggcagtccaa aagaagccgc
     1561 ccatgaaatc ctagccaatc tctatgataa agagaagact gaggataagc caaatacccg
     1621 caatatggtc tggctgacca tcctctctat ccttgccgcc ccaatcggcg tccccctagc
     1681 aatcaccttg gtcgccctct tcatcaccct tctcctaatc atctttagcc tctttctgct
     1741 ccttatctca ctctggattg tcctcctcag tctagcaatc gcccagctac tcctcgcctt
     1801 tgacctcttc acactatcat ggtctacctc cctcctcttc acaggcctgg ccctcatctg
     1861 cttggccctc actctattgg gtagtaaatt cactctagac cttggaagca aaaccttctt
     1921 gcttgtttta cattggatac aaagaaagat tcgcaaagga ggctattatg aaacagctta
     1981 aaccatttac gaaaatcagt ctgagcctgt tcactcttgg aacaattctc agtctcattg
     2041 gctacctggc tggcggttgg acaaatatga aaactgttgc agaaaactat tttcagcccc
     2101 aaacggcaag ctttacggat attcgttcta tagaggtcaa tgaaaaactg tccattatgc
     2161 cctcgcctga caaacaattt cacctccact attaccgaaa tcagcacaaa ggcattgacc
     2221 tcctcagcca ccaagttcaa gatggaaaat taaccatttc tagtgcctat caaggcatcg
     2281 ttacttatga tggtttactc gaacctatct taaatcttgt caatgaccag aatgttgcct
     2341 actaccaacc tgtcttacag gtccctaaaa atagcacaat cgaaacactc tcagggagca
     2401 ttcaaggaga tgtctatctg gataaagtga ctattcaaga tttccaactc tttgttgaaa
     2461 atggtgacat gttgattgaa aacagtcaac tcagctttgc tcatttgtca cacctcaatg
     2521 gagaagtagc aatcaatcat tccacattaa taagtccgaa agacatttcc gatactgtcg
     2581 gcagtagctt aacgattgag gatggggatt ttaaagctga aaacctaaaa ctagaaggaa
     2641 gacatagtat cagtaattac ggtggttcaa tcgacatttc tcttcattcc aaaacaaaaa
     2701 ctgacatcca tatagatgcc agtcaaaata atctgggaat tgaccttcca acctattcca
     2761 atccccaatc cactaattac ctcaacatct ctacactaga tggtgaattg agcattcggt
     2821 aatcgctaaa aagccctcct cacggaaggc ttttctattt atttaaacaa caaaatagag
     2881 atcaatgcca agatagctgg tccaccttgg gttaagataa tttttggact tgctgtaata
     2941 gcaccataag ctgctgcagc aataacacat agaacaaaaa tgcttgtaat ttctaaatta
     3001 ccagtaaaaa taccgtagaa caagaagacc gccaacaagc cattataaat tccttgattc
     3061 ttgaacaatg cagtcacaga cttacgttgt aattcttcct tctccatatt gaatacgcga
     3121 ctagtagagt ctgaatgggt cgcaaaggtt tccaaataca tgatatatag gtgctcaata
     3181 gctacgatac tagctagaat agtggtaaaa atagacataa tgtctcctct caaaaaatta
     3241 tataggtaga ttttgcaagc caatcaatag tttatcaaga gtggctagga attgctcgct
     3301 ctcctcaaca gttagaatct gattcatcga agctttgata gcttggtggt gggcagaagg
     3361 attcgtgatt agcgcttctc tagcctgctc agttggccag acaagtactt ctctctgatt
     3421 gtcaggattt cgcttgcgaa tgatatagcc actctcctcc aacaatttga gatgacgcgt
     3481 aaccgctgcg cggtctatct gaagccgctc ctgcaaagcc aactgatgca ggggtgcatc
     3541 ttttagtaat gtttgcaata ttgaatagcg tgtcaaactg attcccaact gcttttcaaa
     3601 aagattggaa atcgtctcat ctgtcacttt caatctgtat aacaaatctt ccatttcttg
     3661 cattatctac ctcttttgat tgattaatca atcattgatg agtcaattat actacaatcc
     3721 gaacaatttt caactatttt gcctatctat taaaaaaata tgttgcctac acctatattt
     3781 tgttataatt aaggaaataa ctagcagagg gatgggactt acttatggaa aacactccta
     3841 gactaaactt ggtcatagac gtgctcatgc tcgctggtgc cattttggca caaagcggtg
     3901 cagaaattca ccgagttgaa gataccatga ttcggattgc acattcacaa ggaattgaac
     3961 aggccaatgt ccttgccatc cctgctgcta tctttttctc catcgaccat acgaatattt
     4021 cgcgtatgaa acgcattgtc aattcaaatt acgacatgca gaaagtttgt gacgtcaacc
     4081 aaatctcccg ttccctagcc aatgaagaaa tcagcctaga agaagcatgg agccaattaa
     4141 atgcaatcaa acaacaaaag ccaccttata acaatatcca aataacctta gctgccgtag
     4201 tagccgcacc attttttacc atcatgttcg gaggttctct tttagatgca gcaggtgctg
     4261 tcctcgcaac agctttagct ttccctatct ccctaattgt cgagcgctat attggaattg
     4321 attttgtcac caccttcgtt ggcgcctttg tcttctcact cacctcttat ttattaagcg
     4381 caaccactac tctccccatt aatcctaacc tgattaatgc aggggcagtc atgccttttg
     4441 taccagggat tgcttttaca aatgccgtaa gagacctcat gaccaatcat atcaatacag
     4501 ggatgaccaa atttttccaa actgcgctta ttatcttatc gctcggtgca ggtacaactg
     4561 tggcctttat gcttgtgagg taatctatga caccagttca cttcttatta caggcactcg
     4621 cctcttatat cgctatcgca gccttcttaa ttgttctcaa tgttcaaaga aaaatgctgg
     4681 tccctggtgg actcctaggc atgcttgttt ggctcatcta tctttcatta ttaggaccaa
     4741 ctaatgtact gattgctacc tttttcgcag caattatcgg atcctgtgtc agtcaaatca
     4801 tgagtatttg gttaaaaaca ccctccgtca tcttttcatt ggctatactt gcaccacttg
     4861 ttccaggcta tcgagcctac atgaccacga cttatttcgt ttctggggac cacgctcaag
     4921 ccctaaccaa tatcactacc gtcttaactt tagccttagt cattcccatc ggtatggcca
     4981 gcggaacaat attattacac ctttataagg ttcttcgaac aggcaaaaaa gcagcctaac
     5041 cccttgaaaa taaggggcct ttttgttaaa atgaaaactt atcaaaggag atgctatgaa
     5101 ccaaaacgta accaatctaa aacttgctga acgtggtgcc ttgctatcta tcggtgccta
     5161 tattgtctta tcaggtatca aactcgttgc aggacagctc tttcattcgg atgccttacg
     5221 agcagatgcc ttcaataata tttctgatat tatcggtaat atcgctgtac ttgttggact
     5281 aaaaatggca cagaaaccag ctgataccga tcataaattt ggacactgga aaatggaaga
     5341 cttggcaagc ttgattacat ccttcatcat gtttgtcgtt ggtttccaag tactctatga
     5401 taccctccaa aaactaatca gcaattcttc tatcgaagta gacattatgg gggccattgt
     5461 cggaattttt tctgcactag ttatgttagc tgtttattta tataataacc gcttggcaaa
     5521 aaaagtacgt tccaaggcct tagaagcagc tgccaaggat aatctatccg atgcagtcac
     5581 ttccatagga acttctatcg cgatctttgc tgcggctttc aacttcccta ttgtcgataa
     5641 aattgcagcc atcatcatta ccttctttat cctaaaaaca gcctacgata tttttatgga
     5701 aagcttcttt accctatctg atggatttga tgaaaactta ttgaaaaagt acgaggaaga
     5761 tattctcaaa ctcccaaaaa tcgtttctgt caaatcacaa cgtggtcgga cttatggagc
     5821 caatatctac cttgatgttg ttttagaaat gaatccagac ttatccgttt atgaaagtca
     5881 tgaagtgact gagcaggttg agcaactcct aacactcaaa catggagttt ttgatgtgga
     5941 tattcacgta gaaccatctg aaattccaca cgatgagatg tatgagcatg tgtacgacaa
     6001 actcttccgt tttgaaacag agatacaagc acatgctaat gggtatgaag aattgattga
     6061 cgaccagtat ctcttaattg atgccaaagg ccgctatcga aataaaactc agatgcttgc
     6121 cgaccacccc atccaaacca cgtatttatc caattaccaa atgacctcca ttagccaaaa
     6181 atcaaaatta gttacgtttg aaattggtga ttatgttcat acgtcacttt ggcgccgtca
     6241 tgaaaattgg actgttattt tccaccagat ttcaaagaaa caagcctctc agatcgaagc
     6301 tactgaaaaa agctctgatt tgcataatcc ataataaaaa ataggaattt tccctgaatt
     6361 atagggagaa ttcctatttt ttaaccttcc ttttaagcta ttatcagtct tttatactc
//
LOCUS       DBNFCK010000105         6232 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.105, whole genome shotgun sequence.
ACCESSION   DBNFCK010000105 DBNFCK010000000
VERSION     DBNFCK010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6232
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.105"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(33..848)
                     /locus_tag="AC1PIW_001586"
     CDS             complement(33..848)
                     /locus_tag="AC1PIW_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family putative permease subunit"
                     /protein_id="HIP6606496.1"
                     /translation="MIRFLILAGYFEMTMYLYISGKLDQYINLHYSYLAYLSMVLSFI
                     LAIVQLYVWVKEIRVHSHLKSKIAKLSSIGLLLIPLAIAWLFPTVSLDSTTVAAKGYH
                     FPLATENDTNTQNQEGATVQYLKPDTSIYFTKSAYQNQMREIADRYLAEETIVVTNEN
                     YMEVMEAIYDYPTEFSGKTIEMIGFVYNDPDNSQQFFLFRFGIIHCIADSGVYGLLST
                     GATTEFPNNTWVKARGTIKVSYHTSLKQNLPTLELQSMVQIEQPDSPYVYRVF"
     gene            complement(845..1750)
                     /locus_tag="AC1PIW_001587"
     CDS             complement(845..1750)
                     /locus_tag="AC1PIW_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HIP6606497.1"
                     /translation="MLGLDQLPTSVLQAGAIFLSIMIEALPFVLIGAIISGFIDVFIT
                     PDKVRKLLPKNKFLAILFGTFIGFIFPSCECGIVPIVNRLLEKKVPSYTAVPFLVTAP
                     VINPIVLFATFTAFGNSLLFALYRALGAIIVSLVLGIILGFFVKGPILKGSKFDHHQH
                     DYSDKTSWQKVGFALIHAIDEFFDTGRYLVFGCLFASLVQVYIPTAVLTTIGHSPLTA
                     ILLMMLLAFLLSLCSEADAFIGASLLSSFGFAPVMAFLVIGPMVDVKNLLMMKHYFKG
                     KFIVGFIITITLVILGYSLILGGVV"
     gene            complement(1752..1898)
                     /locus_tag="AC1PIW_001588"
     CDS             complement(1752..1898)
                     /locus_tag="AC1PIW_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HIP6606498.1"
                     /translation="MAVTYKRQDDLEKMLEEFASFEKLEEIEYPDPKSKEHSEKNNLN
                     QDKK"
     gene            2021..3979
                     /gene="ligA"
                     /locus_tag="AC1PIW_001589"
     CDS             2021..3979
                     /gene="ligA"
                     /locus_tag="AC1PIW_001589"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIP6606499.1"
                     /translation="MKTRISELVSVLNQYAKEYYQLDQPSVSDAEYDTLYRELVELET
                     AHPELILPDSPTHRVGGKMLDGFEKYSHVYPLFSLQDAFSREELEAFDQRVRKEFPQA
                     TYICELKIDGLSISLTYEAGNLVVGATRGDGSVGENITENLKRVADVPLTLPEAVDIT
                     VRGECYMPKASFDRVNKQRQEAGEAEFVNPRNAAAGTLRQLDTGVVAQRGLATFLYQE
                     ASPSEATSQSQVLEKLDALGFVTNHEYCLAESIDDTWDFIEKIAERRDDLPYEIDGVV
                     IKVNDLAIQEELGFTVKAPRWAVAYKFPAEEKEAEILSVDWTVGRTGVVTPTANLSPV
                     QLAGTTVSRATLHNVDYIAEKDIRIGDTVIVYKAGDIIPAVLKVVDKYRKEQEIMPIP
                     SHCPSCQSDLQHYEDEVALRCINPICPSQLMSKLEHFASRDAMNIAGLGSSIVEKLFG
                     AGLVHDVADIYKLTVDDLLTLEGFKEKSANKLYQAIQTSKSNSAECLLFGLGIRHVGS
                     KASKILVEKFGDLETLAFADQEAIASLEGLGQVIAKSLTTFFASEGAQQLLAELKEAK
                     VNLTYLGQVVDENAALSGMTVVLTGKLERMKRNEAKAKLEALGANVAGSVSKKTNLVV
                     AGTDAGSKLTKAQELGIEIKDEAWLESL"
     gene            3990..5033
                     /locus_tag="AC1PIW_001590"
     CDS             3990..5033
                     /locus_tag="AC1PIW_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HIP6606500.1"
                     /translation="MEERKRARLIYNPTSGQEIMKKNVAEVLEILEGYGYETSAFQTT
                     AEKDSAKNEATRAALAGFDLIIAAGGDGTINEVVNGIAPLEKRPQMAIIPTGTTNDYA
                     RALKVPRGNPVEAAKVIGKQQTILMDIGLAKNQKNGFHQEHYFINIAAAGTLTELTYS
                     VPSQLKTMFGYLAYVVKGAELLPQVQFTPVRVEHDEGVFEGSVSMIFVALTNSIGGFE
                     QIVPDAKLDDGNFTLLMVKTGNLFEILHLIRQVLDGGKHIESDLVEYIKTKSLSIENL
                     NPDNRLLLNLDGEFGGEAPVRLYNLSNHIEFFADTDLVSDHAITLDTDQMNREDMAKR
                     FIEETDHIDEAIK"
     gene            5170..5370
                     /locus_tag="AC1PIW_001591"
     CDS             5170..5370
                     /locus_tag="AC1PIW_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HIP6606501.1"
                     /translation="MSLGALALGLACLGVSIGEGLLVASYLSSTARQPEMQSKLMAGV
                     FLGVAFIEGTFFVTLAMTFVLK"
     gene            5407..6123
                     /gene="atpB"
                     /locus_tag="AC1PIW_001592"
     CDS             5407..6123
                     /gene="atpB"
                     /locus_tag="AC1PIW_001592"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIP6606502.1"
                     /translation="MEEHLSPTLTLGPVTFDLTMVLVSVITISIIFLRVFWASRRMEL
                     KPKGKQNVLEYVYELTINFTKGNLGDAEAKRYSLFFFTVFTFLLVANNLGLMTKLETA
                     EGHNLWTSPTANMAYDFGLATIATVFCHVEGIRRRGFKAYLKSFVTPWAMAPMNILEE
                     VTNLVSLALRLYGNIYAGEVLVSLLLQLSQQSALAYPIAFALNVVWTAFSVFISCLQG
                     YVFIMLVSMYLNKKISSESE"
     gene            6141..>6232
                     /locus_tag="AC1PIW_001593"
     CDS             6141..>6232
                     /locus_tag="AC1PIW_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIP6606503.1"
                     /translation="MATTITMQSSTILGNFILVTASFAVLIILIR"
BASE COUNT         1920 a         1126 c         1373 g         1813 t
ORIGIN      
        1 tgtcagaaaa ttcacttttt tagttggaat tattagaaaa cacggtagac atacggagaa
       61 tctggctgct caatctgaac catcgattgt aattcaagtg ttgggagatt ttgtttcaac
      121 gatgtgtggt aggaaacctt gattgttcct ctggctttta cccaagtatt gttaggaaat
      181 tctgtggtag cccccgtaga gagtaagcca tataccccag aatcagcaat gcagtgaata
      241 ataccaaagc ggaaaaggaa aaattgctgg ctattgtcag ggtcattgta gacaaatcca
      301 atcatttcaa tcgtttttcc tgaaaattcc gtaggatagt cgtagattgc ttccatcact
      361 tccatatagt tttcattagt aacgacaatg gtttcttcag ccaagtatct atcagcaatt
      421 tcacgcatct ggttttgata ggcagatttt gtaaaataaa tagaagtatc aggcttgaga
      481 tactgaacag tcgccccttc ttggttttgt gtattagtat cgttttctgt tgctagtggg
      541 aaatgatagc ccttggcagc aacagttgtt gaatccaggc ttaccgtagg aaagagccag
      601 gctatagcaa gaggaatgag caatagtcca atgcttgata gtttggcaat tttgcttttt
      661 aaatggctat gtactcgaat ttcttttacc cagacataca attgtacgat ggcaagtatg
      721 aaagataaaa ccatggatag ataggctaga tagctatagt gcagattaat gtattggtct
      781 aattttccag agatataaag atacatggtc atttcaaagt aacctgcaag aattaaaaaa
      841 cgaatcatac tacacctcca agtatcagac tatatcccaa aataacgaga gtgatagtaa
      901 tgataaaacc gactataaat ttccctttga aataatgttt catcatgagc aagtttttaa
      961 catccaccat gggtccgata actaagaaag ccattactgg ggcgaatccg aagctggata
     1021 gtagtgatgc accaataaag gcatcggctt cggaacatag ggaaagaaga aaggccagca
     1081 acatcatcag taaaatagct gttaatggac tgtgaccgat ggtagtcagg acagcggttg
     1141 gaatatagac ttgtacgaga ctggcaaata gacaaccaaa gactaggtaa cgtcccgtat
     1201 caaaaaattc atcaatggca tgaataagtg cgaagccaac tttttgccag ctggttttat
     1261 ctgaataatc atgctggtga tggtcaaact tgcttccttt aagaattggt ccttttacga
     1321 aaaaaccaag aatgattccc aatactaggg atacaatgat ggctcctagg gctctataga
     1381 gtgcgaaaag tagggaattt ccaaaagcgg taaaggttgc aaaaaggacg ataggattaa
     1441 tcacaggcgc cgttactaaa aagggaacag cagtataact cggcactttt ttctctaata
     1501 gccgattgac aatagggaca ataccacatt cgcaagacgg aaaaatgaag ccgataaagg
     1561 ttccaaatag aatggcaaga aacttgtttt taggtaagag tttacgaacc ttatctggtg
     1621 ttataaacac atcgataaaa cctgaaataa tagctccaat caagacaaag ggcaaggctt
     1681 caatcatgat agataggaaa atagcccccg cctgtaggac ggaggtaggt aattgatcaa
     1741 gaccaagcat attatttttt atcctgattc aagttgtttt tctcggaatg ttcttttgat
     1801 tttggatctg gatactcaat ttcttccaat ttttcaaatg aagcaaattc ttccagcatc
     1861 ttttctaaat catcttgtcg tttataagtt acagccatag ggcacctcca aaaattttat
     1921 ataaacatta tacatttttt actaaaaatt atctaaagga atctgttttt attgaagcgc
     1981 aaatatagtt cgcaccctca aatcatgata taatattctt atgaaaacaa gaatatcaga
     2041 attagttagc gtgctcaatc aatacgctaa agagtattat caattggacc aacctagcgt
     2101 atcagatgca gaatatgata cgctttatcg tgaattagta gagctagaaa cggctcatcc
     2161 agaactgatt ttaccagata gtcctaccca tcgggtcggg ggaaagatgc tagacggatt
     2221 tgaaaaatat agtcatgttt atcctctttt tagtttgcag gatgcctttt ctcgtgaaga
     2281 gttagaagct tttgaccagc gagttcgcaa ggaatttcct caagcaacgt atatctgtga
     2341 attgaagatt gatggtctgt ctatttccct gacctatgaa gcgggtaatt tagtcgtggg
     2401 tgctacacgt ggtgacggta gtgtcggtga aaatatcaca gaaaatctca aacgagtggc
     2461 tgatgttcct ttgaccttac ccgaagctgt ggacatcact gtccgaggtg agtgttatat
     2521 gccaaaagct tcctttgacc gtgttaacaa acagcgtcag gaggctggtg aagctgaatt
     2581 tgttaatccg cgtaatgcag cagcagggac cctccgccag cttgatacgg gagtggtggc
     2641 ccagcgtggt ctcgctactt tcctctacca agaggctagt ccatctgaag cgactagtca
     2701 gtcacaagta ctggagaaac tggatgctct gggatttgtg accaaccacg aatattgctt
     2761 ggcggagtcg attgatgaca cgtgggattt tattgaaaaa atagcagagc gtagagatga
     2821 tttaccctat gagatagatg gtgttgtcat caaggtaaat gatttagcta tacaagaaga
     2881 actaggattt acagttaaag cgccacgatg ggcagtagct tacaagtttc cagctgagga
     2941 aaaagaagca gaaatcttgt cggttgattg gactgttggc cgaacaggtg ttgtgacacc
     3001 aacggccaat cttagtcctg ttcaactggc aggaacgact gtcagtcgag caactttgca
     3061 taacgtagat tatatcgccg aaaaagatat tcgtattggc gatacggtca ttgtttataa
     3121 ggcgggagat attattccag cagttctgaa agtggtggac aagtatcgaa aagagcaaga
     3181 aatcatgccc attcctagtc attgtccgtc ttgtcagagc gacctacaac actatgagga
     3241 cgaagttgcc ctccgctgta tcaaccctat ttgtcctagc cagctgatga gtaagttgga
     3301 acattttgca agtcgggatg ctatgaatat cgcagggcta gggtcttcaa tcgttgaaaa
     3361 actctttgga gcaggcttgg tacacgatgt tgctgatatt tataaattaa ctgttgatga
     3421 cctattgacc ttggaaggtt tcaaagaaaa atcagctaac aagctctatc aagccattca
     3481 aacttctaag agtaactcag cagaatgcct cctatttgga ctaggtattc gccatgtagg
     3541 tagtaaggca agtaagattt tagtagaaaa atttggtgat ttagaaactc tagcatttgc
     3601 tgatcaagaa gcaattgctt cgctagaagg tcttggtcag gttattgcta agtctttgac
     3661 aacttttttt gctagtgagg gtgcgcaaca acttttagcc gaacttaagg aagctaaggt
     3721 aaacttaacc tatctcggcc aagtagtaga tgaaaatgct gcgctatcgg gtatgacagt
     3781 tgtactgact ggtaaattgg agcgaatgaa acgaaacgaa gccaaagcca aattagaagc
     3841 tttaggggca aatgtagcgg gatctgtttc taaaaaaact aatcttgtgg ttgccggaac
     3901 agatgcaggc agtaaattga ccaaagcaca ggagctaggt attgaaataa aagatgaggc
     3961 ttggttagag agcttgtaaa ggtaaaacga tggaagaaag aaaacgtgct agactaatat
     4021 ataatccgac ctcaggtcaa gaaatcatga agaaaaatgt ggccgaagta ttggaaattt
     4081 tggaaggcta cggttatgag acttcagctt tccaaacaac tgctgagaaa gactctgcaa
     4141 agaatgaagc tacacgtgcg gcgcttgcag gctttgactt gattatcgcg gctggtggag
     4201 atggaacaat caatgaagtc gtcaatggaa ttgctccact tgaaaaacgt ccgcagatgg
     4261 ccatcatacc gactggcacc accaatgact atgcgcgtgc tttaaaagtg cctaggggaa
     4321 atcctgtaga agcagctaag gttattggta agcagcagac aattttaatg gatattggtt
     4381 tagccaaaaa tcaaaaaaat ggttttcatc aagaacatta ttttatcaac attgctgcgg
     4441 caggtacact tacggagttg acctacagtg ttcccagtca acttaagacc atgtttggct
     4501 atttggccta tgtagtaaaa ggagcagaac tcctgccaca agtacaattt acacctgttc
     4561 gtgtagagca tgatgagggc gtgtttgaag gctcggtttc aatgattttt gttgccttaa
     4621 ccaattcaat tggaggtttt gaacagatcg ttcccgatgc taagttagat gatggtaatt
     4681 ttaccttatt gatggttaaa acagggaatc tatttgaaat tctacatctg attcgtcaag
     4741 tcttagatgg tggcaaacac attgagagtg atttagttga atacataaaa accaagagcc
     4801 tatccatcga aaatctgaac ccagataacc gtctactgct caatctagat ggagagtttg
     4861 gtggggaagc ccctgtacgt ctgtacaacc tatcaaacca tatagagttt ttcgcagata
     4921 cagatctggt ttctgaccat gcaatcactc ttgatactga tcagatgaat cgagaagata
     4981 tggccaaacg attcattgaa gaaacagacc atattgatga agctattaaa taggaagaaa
     5041 agatgaaata ctcttttctt tttattttac aaaaacgaat taaacgctta caatggcggt
     5101 attttcttga caaatagatg ttttaggagt atccttacat ggtattaaat tttaaaagga
     5161 gaattcgtta tgagtttagg cgcattggcc cttggattgg cctgtcttgg tgtaagtatc
     5221 ggtgagggac tgttggtggc ttcatacctt agttcgactg cacgtcaacc agaaatgcaa
     5281 agtaaattga tggctggtgt atttttgggt gttgccttta tcgagggaac tttcttcgta
     5341 accttggcta tgacatttgt cttaaaatag tagaaaatag agaaatagaa aaggggggaa
     5401 cctccattgg aagaacattt aagtccaacc cttacccttg gtcccgtaac ctttgacctg
     5461 accatggtat tggtttctgt cattaccatt tccattattt tcttacgagt tttttgggct
     5521 agtcgtcgga tggagctcaa gccaaaagga aaacagaatg ttttggagta tgtttacgaa
     5581 ttgaccatca attttacaaa agggaatctt ggcgatgcgg aagcaaagcg gtattccttg
     5641 ttcttcttta cagtctttac ttttcttttg gttgccaata atctaggttt gatgaccaaa
     5701 ttggaaacag cagaaggaca taatttgtgg acttctccaa cagccaatat ggcttacgat
     5761 ttcggtttgg caacaattgc aacagtattt tgtcatgttg agggtattcg tcgtcgcggt
     5821 ttcaaggcat acctcaaatc ttttgtgaca ccctgggcta tggctccaat gaacattttg
     5881 gaagaagtga ccaaccttgt gtcgcttgct cttcgtctgt acggtaacat ttatgctggt
     5941 gaagtgcttg tctctcttct tctacaatta tctcaacaaa gtgcacttgc atatccaatt
     6001 gcctttgctc tgaacgttgt ttggacagca ttttctgtat tcatttcatg cttgcaggga
     6061 tatgtattta tcatgttggt atctatgtac ttaaataaaa aaatcagtag tgagagtgaa
     6121 taggaaaggg aaagacaact atggcaacaa ctattacaat gcaatctagt accattcttg
     6181 gaaactttat cttggtaaca gcctcatttg cagtacttat tattcttatt cg
//
LOCUS       DBNFCK010000106         6226 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.106, whole genome shotgun sequence.
ACCESSION   DBNFCK010000106 DBNFCK010000000
VERSION     DBNFCK010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6226
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.106"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            42..911
                     /gene="rfbA"
                     /locus_tag="AC1PIW_001594"
     CDS             42..911
                     /gene="rfbA"
                     /locus_tag="AC1PIW_001594"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HIP6606504.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPIYDKPMIYYPLSTLMLA
                     GIKDILIISTPQDLPRFKDMLGDGSELGISLSYAEQPSPDGLAQAFIIGEEFIGDDNV
                     ALILGDNIYHGNGLTKMLQRAASKEKGATVFGYQVKDPERFGVVEFDADMNAISIEEK
                     PEEPKSNFAVTGLYFYDNDVVEIAKNIKPSPRGELEITDVNKAYLERGDLSVELMGRG
                     FAWLDTGTHESLLEAAQYIETVQRLQNVQVANLEEIAYRMGYITKEQVYKLAQPLKKN
                     EYGQYLLRLIGEA"
     gene            911..1504
                     /locus_tag="AC1PIW_001595"
     CDS             911..1504
                     /locus_tag="AC1PIW_001595"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HIP6606505.1"
                     /translation="MTENFFGKTLAARPVEAIPGMLEFDIPVHGDNRGWFKENFQKEK
                     MLPLGFPESFFAEGKLQNNVSFSRKNVLRGLHAEPWDKYISVADGGKVLGTWVDLREG
                     ETFGNTYQTVIDASKSIFVPRGVANGFQVLSDFVAYSYLVNDYWALELKPKYAFVNYA
                     DPSLDIKWENLEEAEVSEADENHPFLKDVKPLRKEDL"
     gene            1504..1989
                     /locus_tag="AC1PIW_001596"
     CDS             1504..1989
                     /locus_tag="AC1PIW_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HIP6606506.1"
                     /translation="MFQAFLEIAEQLNKLGITPLLMGSVGLERRTGRDWQARDLDIHV
                     PSDPRGWEAPDEERIYRADELIVLMNQLGYVLVDRHEHEFHKDGLSVEFGGLHSLPEF
                     AGVELEDLEELETVGVRYYLPTLEQYLKIYQASSKDSYRAENNNQKDFAKIAYLKEIL
                     R"
     gene            2178..3224
                     /gene="rfbB"
                     /locus_tag="AC1PIW_001597"
     CDS             2178..3224
                     /gene="rfbB"
                     /locus_tag="AC1PIW_001597"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HIP6606507.1"
                     /translation="MSQFKNIIVTGGAGFIGSNFVHYVYNNHPDVHVTVLDKLTYAGN
                     KANLEAILGDRVELVVGDIADAELVDKLAAKADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETNYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILAGIKPKLY
                     GEGKNVRDWIHTNDHSTGVWAILTKGRMGETYLIGADGEKNNKEVLELILEKMGQPKD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWTPQFTDFSQGLEETIQWYTDNQDWWKAEKE
                     AVEANYAKTQEVIK"
     gene            3279..4130
                     /gene="rfbD"
                     /locus_tag="AC1PIW_001598"
     CDS             3279..4130
                     /gene="rfbD"
                     /locus_tag="AC1PIW_001598"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019780653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HIP6606508.1"
                     /translation="MILITGANGQLGTELRYLLDERGVDYVAADVAEMDITDADKVDA
                     FFAEVKPSVVYHCAAYTAVDMAEDEGKELNYKINVTGSENVAKATAKYCATLVYISTD
                     YVFNGDLPVGKEWQVDDQPDPQSEYGRTKRKGEEAVEKFADKFYTVRTAWVFGNYGKN
                     FVFTMQNLAETRDTLTVVNDQHGRPTWTRTLAEFMVHLVDTKQAFGYYHLSNDAAEDT
                     TWFDFATEVLKDTNTKVLPVDSSQFPAKAKRPFNSTMSLDKAKATGFVIPTWQEALEA
                     FYKQEKK"
     gene            4147..5190
                     /locus_tag="AC1PIW_001599"
     CDS             4147..5190
                     /locus_tag="AC1PIW_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIP6606509.1"
                     /translation="MKKKAIVKPLLMSGIFLALLGGATLPSGAVPVVAAETSQSTTYH
                     LTDDEKVAVREYIQAKMTIDMQEYRLAFLEGMMEEMASGSAEAAWDEEIADLKANLTA
                     EQVVVLDELEANLIGSIAQHYHYLFETLTVAGKSGREEAAAIVSKYESEDDASTPEAE
                     LAALKYAREVIVELLNKESAAIDNYIAYAEATGQELAGLLESGNSNLESITSATIGYG
                     QALATASQPKFPYDFSEMDRQIAELTASLQSKVEDKSTAKTENTGVQTSQSATNGSND
                     LQTVPDQGGGQISDVATGKGNISEAGQKKVIPNDNAKVLPKTSGKSSLPLTVLGLITI
                     FAGWLLTNKQEEK"
     gene            5258..>6226
                     /locus_tag="AC1PIW_001600"
     CDS             5258..>6226
                     /locus_tag="AC1PIW_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GBS Bsp-like repeat-containing protein"
                     /protein_id="HIP6606510.1"
                     /translation="MFVKKRTLLFLSLCASALNAQLVQADMVNAPSSQVSSSQVAETG
                     GQLSVVDEAGQIKAILSNIQGEIIGVTAQFSSETNSGITVTFSMDEQGRYVAVLDRSA
                     FAEEDQVFSLKLTAQLTDGSFQTLSDYSFEWKKDVVSAAEKPTALDNSDATPSKIEEE
                     ATSTTTSSTVNSSLTAGATSSTSNSLTRSSSNTIAGSSNGSTSVPSARVSTASSSVNV
                     TQPTGTITIENRNDAQGTFDVRVTNVSSPKDISSVILPTWSQTDDLRWYEATRQSDGS
                     YKLTVNKKDHKYRTGTYTVHLYYKDSSGGLTGAGGTTTHLSEAKPTG"
BASE COUNT         1912 a         1211 c         1437 g         1666 t
ORIGIN      
        1 ggtataatga actgatatta atttagggaa ggtactctta tatgaaaggt attattctag
       61 caggtggttc aggtacgcgc ctttatcctc tgacacgtgc ggcttcaaaa caattgatgc
      121 ctatctatga caaaccaatg atttattatc cattgtcaac attgatgttg gctggaatca
      181 aagatatttt gattatttca actccgcaag atcttccacg ttttaaggat atgttaggag
      241 atggttctga acttggaatt tcactttcat atgcagaaca accaagtcca gatggtttgg
      301 cgcaagcctt tattatcggt gaagaattta tcggtgatga caatgtagcc ttgattcttg
      361 gtgataacat ctaccacgga aatggtttga ccaagatgtt gcaacgtgct gctagcaaag
      421 aaaagggagc gactgtcttt ggttatcaag taaaagatcc agaacggttt ggtgttgttg
      481 agtttgacgc tgatatgaat gccatctcta tcgaagaaaa acctgaagag ccaaaatcga
      541 attttgctgt tactggtctt tatttctatg acaatgatgt tgtggagatt gcgaaaaata
      601 tcaagccttc acctcgtggc gagttggaaa tcacagatgt caacaaggct tacttagaac
      661 gcggcgactt gtctgttgag cttatgggcc gtggatttgc ttggttggat acaggtacgc
      721 atgaaagcct cttggaagct gcacagtaca tcgaaacggt tcaacgtttg caaaacgttc
      781 aagtggctaa tttggaagaa attgcttacc gcatgggcta cattacaaaa gaacaagtgt
      841 ataaattggc acaaccgctt aagaaaaatg aatacggaca atacttgctc cgtttgattg
      901 gagaagctta atgacagaaa actttttcgg taaaacacta gcagctcgcc cagttgaagc
      961 tattccagga atgttagaat ttgacattcc tgttcatggc gacaaccgtg gctggtttaa
     1021 ggagaacttc caaaaggaaa aaatgttgcc tctcggcttt cctgaaagct tctttgcaga
     1081 aggaaaattg cagaataatg tttccttctc tcgtaaaaac gttcttcgtg gtcttcacgc
     1141 agagccttgg gataaataca tttcagtagc agacggcggt aaggttttgg gaacttgggt
     1201 cgatctacgt gaaggcgaaa ccttcggtaa tacctaccaa accgttatcg atgcttcaaa
     1261 aagtatcttc gttccgcgcg gagtagccaa tggtttccaa gtcttgtcag atttcgtggc
     1321 ttacagctac ttggttaacg actactgggc tttggaactc aagcctaagt atgctttcgt
     1381 caactacgct gaccctagcc tcgacatcaa gtgggaaaac ctagaagaag cagaagtttc
     1441 agaagcagat gaaaaccacc cattcctcaa ggacgtcaag cctttgagaa aagaggacct
     1501 ctaatgtttc aagccttttt ggaaatagca gagcagttaa acaagttagg aatcactccc
     1561 ttgttaatgg gttctgttgg tctggaaagg cggacaggac gagattggca ggcaagggat
     1621 cttgatattc atgtgcccag tgacccacgg ggatgggaag cgccagatga ggagcggatt
     1681 taccgtgcag atgagctgat tgttctgatg aatcaacttg gctatgtcct ggttgatcgt
     1741 catgaacatg aatttcataa agacggctta tctgttgaat tcggaggcct acattcactg
     1801 cctgaatttg ctggtgtaga gctagaagat ttggaagaac tagaaacagt aggtgttcgc
     1861 tattacctac cgacacttga acaatacctg aaaatctacc aagcctcttc aaaagattcc
     1921 taccgtgcag aaaataacaa tcaaaaagac tttgctaaga ttgcttactt gaaggaaatc
     1981 ttgaggtaaa aaagcgagcg aagcgagcag ttctgtgtgt catcgccgta gtgagaagct
     2041 cctttgcttt gctaaggagc gggggaaacc atgagacctt aggctcatgg tttagtaatg
     2101 aaacaccttg gcggttgctt acgaccccac tacctaagat gacacacaaa aaagaaaaaa
     2161 acttttcgga gaaaaaaatg tctcaattta aaaatattat cgtgactggt ggagctggtt
     2221 tcatcggttc aaactttgta cactatgtat ataacaatca tccagacgtt cacgtaactg
     2281 ttttggataa acttacttat gcaggaaaca aggctaactt ggaagctatt cttggtgacc
     2341 gtgttgagct ggttgttggc gacattgcag atgctgagtt ggttgacaaa ttggctgcta
     2401 aggcagatgc catcgttcac tatgcggcag aaagccacaa cgacaactct ctcaacgatc
     2461 caagcccatt tatccatacc aactttatcg gtacttacac tttgcttgaa gcggctcgta
     2521 aatacgacat ccgtttccac catgtatcga ccgacgaagt atacggtgac cttcctttgc
     2581 gtgaagattt gccaggtcat ggcgaaggtc caggcgagaa atttactgcg gaaaccaact
     2641 ataacccgtc atcaccttac tcatcaacca aagctgcttc agacttgatt gtcaaagcat
     2701 gggtgcgttc atttggtgtc aaagcaacta tctcaaactg ttcaaacaac tacggtcctt
     2761 accaacacat cgagaaattt atcccacgcc aaatcaccaa tatcttggca ggtattaagc
     2821 caaaacttta cggtgaaggt aaaaacgtcc gtgactggat tcataccaac gaccactcga
     2881 caggtgtttg ggcaattttg acaaaaggcc gtatgggtga aacttaccta atcggtgctg
     2941 acggtgagaa gaacaacaaa gaagtgcttg aattgattct tgaaaaaatg ggtcaaccaa
     3001 aagatgctta cgaccacgtg actgaccgtg caggacacga tttgcgctat gctattgacg
     3061 caagcaaact tcgtgacgag cttggttgga caccacaatt cacagacttc tctcaaggtt
     3121 tggaagagac tatccagtgg tatacagata accaagactg gtggaaagct gaaaaagaag
     3181 ctgttgaagc caactatgct aagacacagg aagttattaa ataagattat taaaggctag
     3241 ggctcttgcc caagcctttc ttgaaataag gagcaaatat gattttaatt acaggtgcaa
     3301 atggtcagtt gggtacagaa ttgcgttatc tcttggatga gcgtggtgtt gattacgttg
     3361 ccgctgacgt agcagaaatg gacatcacgg atgcagataa agtagatgcc ttctttgcag
     3421 aagtaaagcc aagcgttgta taccactgtg cagcctacac agctgttgat atggcagagg
     3481 atgaaggcaa ggaactcaac tacaagatta acgtaacagg ctctgaaaat gttgccaagg
     3541 caaccgctaa atactgtgcg accctcgttt acatctcaac ggactatgtc ttcaatggtg
     3601 atttgccagt cggcaaagag tggcaagtag atgaccaacc ggatcctcaa tctgaatacg
     3661 gtcgcaccaa gcgcaagggt gaggaagcag ttgagaaatt tgctgacaaa ttctacactg
     3721 ttcgtacagc ttgggtcttt ggtaactacg gtaaaaactt tgttttcact atgcaaaatc
     3781 ttgcggaaac ccgcgatact ttgacggttg tcaatgacca acacggtcgt ccaacttgga
     3841 cccgtaccct tgcggaattc atggtacact tggtggacac caagcaagca tttggttact
     3901 accatttgtc aaacgatgca gcagaagata cgacttggtt tgactttgcg acggaagtcc
     3961 tcaaggatac caacaccaag gtattgccag tggattctag ccagttccca gccaaggcaa
     4021 aacgtccatt caactcaacc atgagcttgg acaaggcaaa ggcgacaggt tttgtcatcc
     4081 caacttggca agaagcattg gaagcattct ataaacaaga aaagaaatag tctacaggag
     4141 atgtgtatga aaaagaaagc gattgttaag ccgttgttga tgtcaggaat ttttctggct
     4201 ttactaggag gtgctacatt accgtccgga gctgttccag ttgtagcagc agagacaagt
     4261 caaagtacga cctatcattt aactgatgat gaaaaagttg ccgtacgtga atatattcaa
     4321 gctaaaatga ctatcgatat gcaagaatat agattggcgt ttcttgaggg aatgatggaa
     4381 gaaatggctt cgggtagtgc tgaagctgcg tgggatgaag aaattgcaga cttgaaggca
     4441 aacttaacag cagagcaagt tgttgtttta gatgaactcg aagcaaattt aattggtagc
     4501 atagcccaac attaccatta cttatttgag acacttacag tcgctggaaa aagcggaaga
     4561 gaagaagcgg cggctatagt ttctaagtac gagtctgaag atgatgcaag tactcctgaa
     4621 gccgaattgg ctgcattgaa atacgctaga gaagttatcg ttgaactact gaataaagag
     4681 tcggcagcga ttgataatta tattgcttat gcagaagcaa ctggacaaga attagctgga
     4741 ttattggaaa gtggaaactc taatttagaa tctattacat ctgcaactat aggatatggt
     4801 caagctcttg caactgcttc gcaaccaaaa tttccgtatg acttttcaga aatggaccga
     4861 cagattgcag aactaactgc tagtctacaa tcaaaagtcg aggataaaag tacagctaag
     4921 actgaaaata ccggggtaca aacaagtcaa agcgctacaa atggctcaaa tgatttgcag
     4981 accgttccag atcaaggggg cggtcagatt tctgatgtag ctacaggcaa aggcaatatt
     5041 tccgaagcag gtcagaaaaa ggttatccca aatgataatg ctaaggtatt gccaaaaact
     5101 tcagggaaat ctagtcttcc attaactgta ctaggactca taacaatttt tgctggttgg
     5161 ttattgacaa ataagcaaga agaaaaatag aagattagtg taattgcatc tattttgaga
     5221 aatatagttg tttttacatg tgaaatacat aaaggagatg tttgtgaaaa aaagaacgtt
     5281 attatttctt tctttgtgtg catccgcgtt gaatgctcag cttgttcaag cggatatggt
     5341 aaatgcccca tctagtcaag tttcttcatc tcaggttgct gaaactggcg ggcagttatc
     5401 tgtcgttgat gaggctggtc agattaaagc tattctatcg aacattcaag gggaaattat
     5461 tggtgttaca gcccagttta gctctgaaac aaatagcggc attactgtca cattctcaat
     5521 ggatgagcag ggtagatacg tagcggtttt ggatcggtct gcatttgcgg aggaagacca
     5581 agtcttttcg ctgaagttaa cagcacaatt aacagatgga agttttcaga ccttatcaga
     5641 ttatagtttc gagtggaaga aggatgtcgt aagcgcggct gagaagccta ctgctttgga
     5701 taatagtgat gcaactccgt ctaaaattga ggaggaagca acttcgacta ctacaagctc
     5761 aacggttaat tctagtttga cagcaggtgc aacaagtagt acttcgaatt cgctgacaag
     5821 aagttcttca aatacgattg caggaagttc gaatggctct acaagtgttc caagtgctcg
     5881 agtcagtaca gcttccagtt cggtcaatgt tacacagcca acaggtacca tcacaattga
     5941 aaatcgtaac gatgcccaag gcaccttcga tgttcgtgtt actaatgttt catcaccgaa
     6001 agatatatca agtgttattt tacccacttg gtcacagacg gatgatttac gttggtatga
     6061 agccactcgt cagtcagatg gcagttataa gttaactgtt aataagaagg accataagta
     6121 tcgtacaggt acctacacgg ttcatttata ttataaggat tcaagtggtg gcctaacagg
     6181 tgcaggtgga acaacaactc atttatcaga agcgaagcca acaggt
//
LOCUS       DBNFCK010000107         6134 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.107, whole genome shotgun sequence.
ACCESSION   DBNFCK010000107 DBNFCK010000000
VERSION     DBNFCK010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6134
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.107"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..965)
                     /locus_tag="AC1PIW_001601"
     CDS             complement(<1..965)
                     /locus_tag="AC1PIW_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HIP6606511.1"
                     /translation="MEVLYQSCAGIDVHQANIVVCILHGPLTSTRPKREMATFDTTTK
                     GLRACHDFLSQFHVEAVGMESTGVYWRPVWHALCDDFELILAQPAHMKAIPGQKTDKK
                     DAHWIAKLTRIGLLPRSFVPDETIQELRELTRQRKHYVESRNRETNRIHKILQSGGIK
                     LTTYIEDIMGLSGRNLLQLLVDGTPITPRIVHQSVYTSLKKKVPQLLEALDDYFSDHH
                     RFMLKQSLEIYDFYQKQIELLEERMNVYLSQYEKHVEILDSIPGIDVITASVIISEVG
                     VDMSQFPTFGHLASWAGLCPGNNESAGKKRSTKIRHGNSYLKKCL"
     gene            complement(1193..2473)
                     /locus_tag="AC1PIW_001602"
     CDS             complement(1193..2473)
                     /locus_tag="AC1PIW_001602"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HIP6606512.1"
                     /translation="MSTFSGFYKKSRQERIDILRQNRSLAEDSLDILYKDENLPEAIA
                     GKMAENHLGTFSLPFSVLPELLVDGQTYSVPMVTEEPSVVAAASFGAKIIAKSGGFTT
                     TIHNRIMIGQVALYDISDHSRATQAILDHKESILENANQAHPSIVKRGGGARELTVES
                     KDEFLIVYLQVDVQEAMGANILNNMLEAVKDDLEELSKGQALMGILSNYATESLITAQ
                     CHIAISSLATSSAIAQETAQKIALASKLAQVDPYRAATHNKGIFNGIDAVVIAAGNDW
                     RAVEAGAHAYASRDGQYKGLSTWSIDGEHLVGSITLPLPIASVGGSIGLNPKVAVAFD
                     LLQQPKARQLASIIASVGLCQNFAALRALVTSGIQAGHMKLHAKSLALLAGAEEHEVD
                     QLAQLLRKEKHSNLETAQNLLAKMREHWEWERHP"
     gene            complement(2535..3689)
                     /locus_tag="AC1PIW_001603"
     CDS             complement(2535..3689)
                     /locus_tag="AC1PIW_001603"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HIP6606513.1"
                     /translation="MNIGIDKIGFAAPDYVLDLADLAQARNVDPNKFKIGLLQSEMAV
                     APVTQDIISLGAKAAEAILTEEDKQTIDMVIVGTESSVDQSKAAAVTIHGLLGIQPFA
                     RSIEMKEACYGATAGLSLAKSHIAQFPESKVLVIASDIAKYGVASGGEPTQGAGAVAM
                     LVTANPRILVLNNDNVCQTRDIYDFWRPNYDKYPRVDGKFSTEQYTDCLTTTFDYYQQ
                     KTGKTLNDFAAMCLHIPFSKQGLKGLQAIAQDEETLSRLTERFQEAIVYNKVVGNIYT
                     GSIFLSLLSLLENSRALETGDQILFYSYGSGAVCEIFSGQLVEGYRNHLQENRLEQLN
                     QRTKLSVKEYEQVFFEEITLDETGSSLDLPEDQSPFALIKVDNHKRIYRK"
     gene            complement(3718..4902)
                     /locus_tag="AC1PIW_001604"
     CDS             complement(3718..4902)
                     /locus_tag="AC1PIW_001604"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HIP6606514.1"
                     /translation="MKKVAIVSAYRSAIGSFGGSLKDIEIADLGAQVLETALASKNIP
                     ADSVDEVIFGNVLSAGQGQNIARQIAIRAGIPQTASAYAVNKVCGSGLKSVLLAAQSI
                     MLGDNDVVVAGGIEIMSQAPYLSKTSRFGSKFGHITLEDSMLTDGLTDAFNDYHMGIT
                     AENVAEHYQVSRAEQDAFTYSSQEKAAKAIAEGRFVDEIVPIRLKNRKGETIFATDEY
                     PRLTPIEKLATLRPSFKKDGTVTAANASGINDGCAVLILMSDEKASELNIQPLTYIEA
                     YATSGLDPALMGLGPITASQKALQKLNKTVEDIDLFELNEAFAAQSIPVVKQLGIDPA
                     KVNVNGGAIALGHPIGASGSRILVTLIHELIKQEKELGLCSLCIGGGQGISLIVSNAQ
                     TS"
     gene            5128..5820
                     /locus_tag="AC1PIW_001605"
     CDS             5128..5820
                     /locus_tag="AC1PIW_001605"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HIP6606515.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDLAFTSVLKRAIKTTNLALEAADQLWVPVEKSWRLNERHYGGLTGLNKAEAA
                     AEFGDEQVHIWRRSYDTLPPEMAKDHEHSAHTDRRYAHLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKQLSDDEIMDVEIPNFPPLVFELDE
                     NLNIVKEYYLEA"
     gene            6007..6134
                     /locus_tag="AC1PIW_001606"
                     /pseudo
     CDS             6007..6134
                     /locus_tag="AC1PIW_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005576373.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1684 a         1296 c         1384 g         1770 t
ORIGIN      
        1 cataaacatt tcttcaaata tgaatttccg tgtcgaatct tggtacttcg ttttttacca
       61 gcactctcat tattacctgg acagagtcca gcccaagaag ctaaatgtcc aaatgtcgga
      121 aactgactca tgtcaactcc aacctcagaa atgataacag aagctgtaat gacatcaatg
      181 cctgggatgg aatccaatat ttctacatgt ttttcatatt gtgatagata gacattcatt
      241 cgctcttcca acaactcaat ctgcttctga taaaaatcat aaatctctaa ggactgcttt
      301 aacataaagc ggtgatggtc agaaaaatag tcatccaagg cttccagaag ctgcggcact
      361 ttcttcttca agctggtgta aactgattga tggacaatgc gaggtgtaat aggcgtccca
      421 tcaaccagta gctgaaggag attgcgacca gatagcccca taatatcttc aatataggtt
      481 gttagcttga taccacctga ctgaagaatt ttatggatac ggtttgtttc tcgattgcga
      541 ctttccacat aatgttttcg ttgtctggtc aactccctca attcttgaat ggtttcatcg
      601 ggaacgaaac tccgaggaag cagaccaatc cgtgttaatt tggcaatcca gtgagcgtcc
      661 ttcttgtcgg ttttctgacc tggaatagcc ttcatgtggg ctggttgagc gagtatcaac
      721 tcgaagtcat cacatagagc atgccagaca ggtcgccaat agacaccggt gctttccata
      781 ccaacagctt ccacatgaaa ttgactgaga aaatcgtggc aagcacgtag gcctttagtc
      841 gttgtatcaa atgtagccat ctcacgcttt ggacgagttg aggtgagtgg tccatgtagg
      901 atacagacga caatattggc ttgatggaca tcaatacctg cacaagattg atagagaacc
      961 tccatgataa ttccctcctt ctaaaaatta gagagcttat ctaccgaatt gtagtcgtat
     1021 ttttatactc atgcctatcg catattttgg ggtgctcgtc agtagagtgg gttagttttt
     1081 cccgcgatga ctaacatcag taactcgtca accacaaagc cctcactaag attatacacc
     1141 tagtgagggc tttatgttcg ttttcattat tctgtgggtg agggcggagc catcatggat
     1201 gtctttccca ctcccaatgt tctctcattt ttgctaataa gttttgagca gtttctaggt
     1261 tgctgtgctt ttctttccgc aggagttggg ctagttggtc tacttcatgc tcctcggcgc
     1321 cagcaagtag cgctagggat tttgcgtgga gcttcatgtg accagcctga atccctgaag
     1381 taacaagagc acgcagggca gcaaagttct gacagagacc gacagaagcg atgatagaag
     1441 ctaattggcg tgcctttggt tgttggagaa ggtcaaaagc gactgctacc tttggattga
     1501 gcccgatcga gccgccgacg gaggcgatcg gaagaggtag ggtgatggag ccgaccaagt
     1561 gctcgccatc aatagaccaa gtagaaagcc ccttgtattg tccatcacgg ctggcataag
     1621 catgggctcc ggcttcgact gcccgccagt catttcctgc agcgataacg acagcatcta
     1681 taccattaaa aatccctttg ttatgtgtcg cagcacgata agggtccact tgcgccagtt
     1741 tgcttgccag ggctattttt tgagcagttt cttgagcaat ggcagagctg gtagccagac
     1801 tagagatggc aatatggcat tgggcggtga tcagtgactc cgttgcatag ttggagagga
     1861 ttcccatcaa agcctgtccc ttgctgagct cttctaagtc atccttaaca gcttctaaca
     1921 tgttattgag gatattggct cccatggctt cttgtacatc aacctgaaga tagacaatca
     1981 aaaactcatc cttactctca acagtcaatt ctcttgcacc gccccctcgt ttgacaatgg
     2041 aagggtgtgc ttgattggca ttttctaaaa tactctcctt atggtcaaga atcgcctgtg
     2101 tcgctctgct atggtccgaa atatcgtaga gagcaacttg tccaatcatg atacggttgt
     2161 gaatagtcgt tgtaaaaccg ccagatttag cgatgatttt ggctccgaaa gatgcagctg
     2221 caaccacaga aggttcttca gtaaccatcg gtacagagta agtttgtcca tccactagaa
     2281 gttctggaag tacagaaaaa ggtaaggaaa acgttcctag atgattttct gccatctttc
     2341 ccgcaattgc ttctggcagg ttttcgtctt tgtatagaat gtctaagcta tcttcggcta
     2401 gggaacgatt ctgacggaga atatcaatgc gctcttggcg tgatttttta taaaatccgg
     2461 aaaaagttga cataggttcc tctttctata ggaaagcgcc ccttggtgag agccaaaggg
     2521 cgccgttttg gcttttattt acgatagata cgtttgtgat tatccacttt gataagggca
     2581 aagggacttt ggtcttctgg taggtcaagg gatgaaccgg tttcatcaag ggtaatttct
     2641 tcaaagaaga cttgttcgta ttccttaacg ctgagttttg tacgctgatt gagttgttct
     2701 aaacgatttt cttgtaagtg gttgcgataa ccttcaacga gttgaccgct gaaaatttca
     2761 cagacagcgc cgctaccata gctataaaag aggatttgat cccctgtctc taacgctctg
     2821 ctattttcca aaagggaaag aagagagagg aagatagagc cagtgtagat gttgccgacg
     2881 actttattat agacgatagc ttcctggaaa cgttctgtca agcgactaag agtttcttca
     2941 tcctgagcaa tagcttgcag tcctttaagc ccttgcttgg agaaaggaat atgtaagcac
     3001 atggcagcaa aatcgttgag ggttttgcct gttttttgtt gatagtagtc gaaagttgtc
     3061 gtcaagcaat cggtgtactg ttcagttgaa aatttaccat caacacgtgg atatttgtcg
     3121 tagttgggac gccagaagtc gtagatgtca cgtgtctggc agacattatc attgttgagg
     3181 accaaaattc gtgggtttgc ggttaccaac atggcaactg ccccagcacc ttgggtaggt
     3241 tcaccgccag aagcgactcc atactttgca atatcactag caatgactag gacttttgat
     3301 tctgggaatt gagcgatatg gcttttggca agacttagtc cagccgttgc accgtaacaa
     3361 gcctccttca tctcgatgga gcgagcgaag ggctgaatac cgagtaatcc atgaatggtg
     3421 acggcggcag ccttactttg atctacgcta gattctgtgc cgacaataac catgtcaatg
     3481 gtttgtttat cttcttctgt cagaatggct tcggcagcct ttgccccaag actgataata
     3541 tcttgagtga caggagctac agccatttca gactgaagaa gaccaatctt aaatttattt
     3601 gggtccacat tgcgggcttg ggctaaatca gccaaatcta aaacataatc gggtgccgca
     3661 aatccgattt tatcaatacc gatgttcata atatatcctt tgtgtacaaa tgataggtta
     3721 acttgtttgc gcattggaaa cgattaggga gatgccttga ccgcctccga tacaaagtga
     3781 gcaaagacca agctcttttt cttgcttgat taattcgtga atcagagtga ctaggattcg
     3841 actaccacta gctccgattg gatgtccaag ggcgatagca ccaccattga cattgacttt
     3901 tgcaggatca atgccgagtt gtttgacaac tggaattgat tgggctgcaa atgcctcgtt
     3961 gagttcgaat agatcgatat cctcgactgt tttattcagt ttttgtagcg ctttttggct
     4021 agcagtaata ggtccaagcc ccatcaatgc gggatctaaa ccgcttgttg cataggcctc
     4081 aatataggtg agtggctgga tatttagctc actggctttt tcatcagaca tgagtatcaa
     4141 gacagcacag ccgtcgttga tgccagaagc attggctgcg gtgacggttc catccttttt
     4201 aaaagatggc cgaagtgttg ccaatttttc aattggcgtc agacgagggt actcatctgt
     4261 ggcaaaaata gtttcgcctt tccgattttt taatctaata ggaacaattt cgtcaacaaa
     4321 tcttccctca gcaatagctt tcgcagcctt ctcttggcta ctgtatgtaa aagcatcttg
     4381 ctctgcgcga ctgacttgat agtgttcagc aacattttct gcagtaatgc ccatgtgata
     4441 gtcgttgaag gcatctgtta gaccgtctgt cagcatagaa tcttctagag taatatgacc
     4501 aaatttacta ccaaacctgc tagttttgga tagataaggt gcctggctca taatttcaat
     4561 gcctccagcc acaactacat cattgtcacc tagcatgatt gactgggcag ccaggaggac
     4621 tgatttgaga ccggaaccac agactttatt gactgcatag gcagaggctg tctgaggaat
     4681 gcctgcacga atagcaattt gacgtgcaat attttgtcct tgtcctgctg aaagaacatt
     4741 tccgaagata acttcatcaa cgctgtctgc agggatgttt ttactagcta aggctgtttc
     4801 gaggacttgc gcccctaaat cagcgatttc aatatctttc agactacctc cgaaactgcc
     4861 gatggcagaa cgataggcag acacgatagc tacttttttc atgataactc ctatagtggt
     4921 atcgttttga actgtcgtgt tcagccttac tattatacca ttattttgta ttttttctaa
     4981 gaagaaagat aggtctagag actgaattta catgaaagag taatgaaagc gtacacagat
     5041 tgtggaaagg ctggcatttt tttacctttt cgtgtaaaat agagaagtat aagaggggaa
     5101 cctcaaaatt taaaggagaa ttcataaatg gtaaaattgg tttttgctcg ccatggtgag
     5161 tctgaatgga acaaagctaa ccttttcact ggttgggctg atgttgattt gtctgaaaaa
     5221 ggtacacaac aagcaatcga tgcaggtaaa ttgatcaaag aagcaggtat cgaatttgac
     5281 cttgctttca catctgtatt gaaacgtgct atcaagacta caaacttggc tcttgaagct
     5341 gcagatcaat tgtgggtacc agttgaaaaa tcgtggcgtt tgaacgaacg tcactacggt
     5401 ggtttgactg gcttgaacaa agctgaagca gctgctgaat ttggtgatga gcaagttcac
     5461 atctggcgtc gttcttatga tactttgcca ccagaaatgg ctaaagatca tgagcattca
     5521 gcacacactg accgtcgcta tgcacacctt gatgattcag ttattccaga tgcagaaaac
     5581 ttgaaagtaa cgcttgagcg tgcccttcca ttctgggaag ataaaattgc tccagcattg
     5641 aaagacggta aaaacgtatt cgtgggtgca cacggtaact caatccgtgc ccttgtaaaa
     5701 cacatcaaac aattgtctga tgacgaaatc atggatgtgg aaattccaaa cttcccacca
     5761 cttgtattcg aattggatga aaacttgaac attgtgaaag agtactactt ggaagcataa
     5821 ttcatttaag gcctgagttc gctcaggctt ttttagttat gtgactttta gtccgtcacg
     5881 ctccgtaagg tgcgtgacaa ataaaccacc cgctatgcgg gtgcgcgacg aaggttacac
     5941 caaaaaaact ccaaacgcga tacaataaag gtgttcaagc ctattgtaaa gcgaaaggag
     6001 aaaaatatgg cgcaaaaggc acatagttta tcacatacaa agtggctgtg ttctaccaca
     6061 ttgtcttcac acttaagtat agacgaaaaa tcatctataa tcaatatcga agtagtttgg
     6121 gagaaatatt ccga
//
LOCUS       DBNFCK010000108         5999 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.108, whole genome shotgun sequence.
ACCESSION   DBNFCK010000108 DBNFCK010000000
VERSION     DBNFCK010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5999
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.108"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..450
                     /locus_tag="AC1PIW_001607"
     CDS             <1..450
                     /locus_tag="AC1PIW_001607"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIP6606516.1"
                     /translation="TDRDICVFPSSMETFGLVYVEAILRGLPVILSNNLGHLTAFEIF
                     SAGQIYKLGDIEELVTKLEQVIESFRNFSDRARLDVERLRTLYTPEVAYKEILDGINH
                     LEYTGHQNTNLSHIKELLTLNMRPTRLERYARLISNMATKIRHKLRL"
     gene            554..1270
                     /locus_tag="AC1PIW_001608"
     CDS             554..1270
                     /locus_tag="AC1PIW_001608"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIP6606517.1"
                     /translation="MKVLMIIPAYNEEESILQTVQGIIDYKNSVNFQLDYVVINDGST
                     DSTKEILIQNKLNAVHLVQNLGIGGAVQTGYKYALDNDYDVAVQFDGDGQHDIRSLNG
                     LIQPILVGQADMVIGSRFVGDTLSEFQTSFMRRFGIGVISNMIKLTTGNRIWDTTSGY
                     RLGNRKVIAQFAKRYPIKYPEPESTVHLLKQNFQVVEAPANMFERAGGVSSITPFKSI
                     RYMVEVCSSILIASLMKEGE"
     gene            1270..1626
                     /locus_tag="AC1PIW_001609"
     CDS             1270..1626
                     /locus_tag="AC1PIW_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HIP6606518.1"
                     /translation="MTIQALAMFLASLGFLYFIFRNINKNKILFEHAFMWIVIGFGLI
                     VFALFDVIPIKLAYLFGFGLTSNFLLSVAIFVLLVIGFLHSMALSQQKQQIKNLIQEV
                     SMAKKRISELEEHHAE"
     gene            1616..2386
                     /locus_tag="AC1PIW_001610"
     CDS             1616..2386
                     /locus_tag="AC1PIW_001610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIP6606519.1"
                     /translation="MQNNHTFVICAYGDSKYLEECIHSLKQQTLQSQLILYTSTPSEY
                     IEQICQKNAIVMHTAQGGGIGKDWNLALSFVTTPYATIAHQDDIYLPDYCEKIMSQMT
                     EETLIAYSDYQEVKEGVAIPLTSNLKIKQLMLRTMAMFPKWKFWRNRVLAFGNPISCP
                     AVTYNLKKLNDFKFNEEMKVSLDWFAWYQIAQKNGSFTFVDESLMYHRIHEESETTNS
                     IENNIRTQEDYEMYLLFWPKFIAKFLLSYYVKSQETNN"
     gene            2569..3057
                     /locus_tag="AC1PIW_001611"
     CDS             2569..3057
                     /locus_tag="AC1PIW_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HIP6606520.1"
                     /translation="MIKIFGKIRYHWQPDLSWAIIYWSLTFTPIFIGMALLLEKLQVS
                     RLFILLVSLFAVLVVLGLHRYFEIKDEHLRIASANPFAVQKIEIATIEKIEVSYLAIR
                     IFAKEFPNGRMYYMRKWTKKYFINHLAIHSCFKGKIELIDHLIKQDYFEEYYTKKAKS
                     IR"
     gene            complement(3046..3240)
                     /locus_tag="AC1PIW_001612"
     CDS             complement(3046..3240)
                     /locus_tag="AC1PIW_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HIP6606521.1"
                     /translation="MKVKLIPERCIACGLCQTYSSLFDYDDDGIVVFSDDSQFEKTIE
                     PNPDILKAIKSCPTKAISAD"
     gene            3291..3767
                     /locus_tag="AC1PIW_001613"
     CDS             3291..3767
                     /locus_tag="AC1PIW_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HIP6606522.1"
                     /translation="MSQEPWNEEVYHTETTSRKERLSKGVANTTVFTVLAIIFFVIVL
                     IIGIMAVYLSIGGGSKTDSTKEFYTPSTTTVVAESSSSAAETQESTSTDTTETETSSS
                     PAEATDGSTLTVQAGEGVGQLAARGGISIAELERLNPEKMTTGSWIAHPGDVVRIR"
     gene            3777..4454
                     /gene="cmk"
                     /locus_tag="AC1PIW_001614"
     CDS             3777..4454
                     /gene="cmk"
                     /locus_tag="AC1PIW_001614"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIP6606523.1"
                     /translation="MKSIQIAIDGPASSGKSTVAKIIAKNLGYTYLDTGAMYRSATYL
                     ALTNGIEVTDQNRIVDLLAQYPIRFGRDENGQQLVFVGDEDVTLPIRDNQVTNNVSAV
                     AALPLVREELVRLQQDIAQAGGIVMDGRDIGTVVLPQAELKIFLVASVEERALRRFKE
                     NTERGIETDLESLKEEIAARDYKDSNREVSPLKAADDAITFDTTGVSIEGVVKFISEK
                     AKEILDR"
     misc_feature    4481..4609
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            4650..5156
                     /gene="infC"
                     /locus_tag="AC1PIW_001615"
     CDS             4650..5156
                     /gene="infC"
                     /locus_tag="AC1PIW_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HIP6606524.1"
                     /translation="MFINDEIRVREVRLIGLEGEQLGIKPLNEAQAIADSANVDLVLI
                     QPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPVIDKGDFETKLRHA
                     RKFLEKGNKVKVSIRFKGRMITHKEVGAKVLAEFAEATQDIAIIEQRAKMDGRQMFMQ
                     LAPASDKK"
     gene            5183..5383
                     /gene="rpmI"
                     /locus_tag="AC1PIW_001616"
     CDS             5183..5383
                     /gene="rpmI"
                     /locus_tag="AC1PIW_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HIP6606525.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKAGMVHAGDFKRIKSMLTGLK"
     gene            5427..5786
                     /gene="rplT"
                     /locus_tag="AC1PIW_001617"
     CDS             5427..5786
                     /gene="rplT"
                     /locus_tag="AC1PIW_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIP6606526.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHLLFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AAAFTALADAAKAKLAK"
BASE COUNT         1941 a         1006 c         1231 g         1821 t
ORIGIN      
        1 actgatagag atatctgtgt tttcccctca tctatggaaa cttttggctt agtctatgtt
       61 gaagcaatct taagagggct tccagtaatt ctatcaaata atttgggaca tttgacggca
      121 tttgaaatat tctcagcagg tcaaatttat aaattgggcg atatagaaga gttggtgact
      181 aagttagagc aagtgattga atcctttaga aatttttcag atagggctcg gcttgatgtg
      241 gaacgtttac gtactctata tacaccggaa gttgcctata aagaaatact ggacggcatc
      301 aatcacttag aatatactgg acaccaaaat acaaatttaa gtcacataaa agagttactg
      361 actttaaata tgcgtccaac aagattggag cgttatgcaa gattgatcag caatatggcg
      421 acaaaaatca gacataaact tcgactttaa tgaataatag gatagctatg ctgaaagatg
      481 tatattatag tttcttcaaa tgagtaagta gaatctaaaa agttatatag aattctagtt
      541 ttagagagaa aaaatgaaag tattaatgat tatccctgcc tacaatgaag aagaaagtat
      601 ccttcaaact gttcagggaa ttatagatta taaaaatagt gtgaatttcc aattggatta
      661 tgttgtcatc aatgatggtt caacagatag cacgaaagaa attttgattc aaaataaatt
      721 aaatgctgtt catctagtac aaaatttagg tattggtgga gctgttcaga cgggatataa
      781 atatgccttg gacaatgact atgacgtagc agttcaattt gatggtgatg gtcagcatga
      841 cattcgttca ttaaatggct taattcagcc aatcttagtt ggtcaggctg atatggtgat
      901 tggctcacgc tttgttggtg atactttgtc ggaatttcaa actagcttta tgagacgatt
      961 tgggataggt gttatttcaa atatgattaa attaacaact ggaaaccgaa tttgggatac
     1021 tacatcaggt tatcgtttgg gaaatagaaa agtgattgca caatttgcaa aacgataccc
     1081 aattaaatat ccggaaccgg aatcaactgt tcatttacta aagcaaaatt tccaagtagt
     1141 agaagcaccg gccaatatgt ttgaaagagc gggaggggtt tcttcgatta ctccatttaa
     1201 gtccatccgc tacatggtag aagtttgctc atctattttg attgcttcgc ttatgaagga
     1261 gggggaataa tgactataca agcattagct atgtttcttg cctcgctagg ttttttgtat
     1321 tttattttta ggaatattaa taaaaataag atcttattcg aacatgcctt tatgtggatt
     1381 gttattggct tcggactgat tgtttttgca ctctttgatg ttattcctat taaattggcc
     1441 tacttattcg ggtttggtct gacttccaat ttcttgttat ctgtggcaat ttttgttctc
     1501 ttggtaattg gatttttgca tagtatggct ttatctcaac aaaagcagca gattaaaaat
     1561 ttaattcaag aagtatcaat ggctaaaaaa agaatctctg aattggagga gcatcatgca
     1621 gaataaccat acatttgtta tctgtgctta tggagatagt aagtatctag aagaatgcat
     1681 ccattcgtta aagcagcaaa cacttcaatc acaactcatc ctatacacta gtactccgag
     1741 tgaatatata gaacaaattt gccaaaagaa tgcaatcgta atgcataccg cacaaggagg
     1801 aggtattgga aaagattgga atttggcact ctcttttgta acaacccctt atgctacaat
     1861 tgctcatcaa gatgatattt atttaccaga ctattgtgaa aaaataatgt ctcagatgac
     1921 agaggaaacg ttgattgctt attcagatta ccaggaagtt aaggaaggtg tggctattcc
     1981 cttaacttca aatttgaaaa tcaagcaact catgttacgg acaatggcca tgtttcccaa
     2041 atggaagttt tggcggaata gggttttagc ctttggtaat ccaatttctt gtccagctgt
     2101 gacctataat ttgaagaaac tgaatgattt caagtttaat gaagaaatga aagtttctct
     2161 tgattggttt gcttggtatc agattgcaca aaaaaatggg agctttacct ttgtagatga
     2221 gagtttgatg tatcatcgta tccatgagga atctgagacg acaaatagca ttgaaaacaa
     2281 tattcgtaca caagaagact atgaaatgta tttgttgttc tggccaaagt ttattgccaa
     2341 gtttctcctg agttactatg ttaagagtca agagacgaat aattaaaata ttttagttac
     2401 caatcattgt attcccatca attcctactc aaaatatttc aaaaacattt cagaaaaatt
     2461 acaaaacatt caacattgtc atgtaaatgt aataaaaaat ttcaaaagat agaggttttc
     2521 ataggaaaat ctcttttttt gtgctagaat aatagatgga ggtccgatat gatcaaaatt
     2581 tttggtaaaa ttcgttacca ttggcaaccg gatttatcat gggccatcat atactggtca
     2641 cttaccttca cacctatttt tattggtatg gcacttttat tggagaagtt gcaagtttca
     2701 agattattta ttctacttgt ctcactcttt gctgtattag tagtgctcgg cttacatcgc
     2761 tatttcgaga taaaagatga gcatttacgg attgcttcgg ctaatccttt tgcagtgcag
     2821 aaaattgaaa ttgctaccat agaaaaaatt gaagtttctt atttagccat tcgtatattt
     2881 gcaaaagaat ttcccaacgg acgaatgtat tatatgcgta aatggacaaa aaaatacttt
     2941 attaaccatt tagctattca tagttgcttc aagggtaaaa ttgaattaat agaccattta
     3001 ataaaacagg attattttga agagtattat acaaaaaaag ccaaatcaat ccgctgaaat
     3061 ggcttttgta ggacatgatt tgatggcttt gagaatatct ggattgggtt caatcgtttt
     3121 ctcaaattgt gagtcgtctg aaaaaacaac gataccgtcg tcatcataat cgaatagaga
     3181 tgagtaggtt tggcagagcc cgcaagcaat gcaccgttca ggaataagtt ttactttcat
     3241 attagtattg taatcatatt ttaataaaaa tacaaggagt tttctcaata atgtcacaag
     3301 agccatggaa tgaagaagtt taccacacag aaacaactag tcgtaaagaa cgtttgagta
     3361 agggggtagc gaatacaaca gtatttacag ttttggcaat tattttcttt gttatcgtac
     3421 tgattattgg aattatggct gtctatctct caatcggtgg cggtagtaaa acagattcaa
     3481 caaaagaatt ttatacccca agcactacaa cagtcgtagc agagagctct tcatcggctg
     3541 ctgagacaca agaatctact tcgactgaca cgacagaaac agaaacaagt tctagcccag
     3601 cggaagcaac ggatggctct acccttactg ttcaagcagg tgaaggtgta ggacaactgg
     3661 ctgctcgtgg tgggatttca attgcagagc tagagcgttt gaatccagag aaaatgacta
     3721 cagggtcttg gattgctcat cctggggatg tagttcgtat tagataaaga ggagttatga
     3781 aatcaattca aattgcaatt gacggtccag cttcaagtgg aaagtcaacg gtcgctaaaa
     3841 ttattgctaa aaatttggga tatacttact tagatacagg agctatgtat cgctcggcta
     3901 cctatctagc cttgacaaac ggcatagagg tcacagatca aaatagaatc gtggacttgc
     3961 ttgcccagta tcccattcga tttgggcggg atgaaaacgg tcagcaactt gtttttgtcg
     4021 gtgatgagga tgttactttg cctattcgag ataatcaagt aacaaataat gtatcagcgg
     4081 tagcagcgct tcctctagta cgggaagaat tggtccgcct gcaacaggac attgcgcaag
     4141 ctggtgggat tgttatggat ggtcgtgata ttggtacggt tgtattgccg caagcagaac
     4201 tcaaaatatt ccttgttgcc tctgttgaag agcgtgccct tcgtcgattt aaggaaaata
     4261 cggaacgtgg cattgagaca gacttggaat ccttgaaaga ggagattgcg gctcgtgact
     4321 ataaagattc caatcgagaa gtttctcctt taaaggcagc agatgatgct attacctttg
     4381 atacaacagg agtatctatc gaaggtgtag tgaaatttat ttccgaaaaa gcgaaagaaa
     4441 ttcttgacag gtagctttag acatgttaga atagtcgtat tgagaaaagc agaagtgaga
     4501 acttctcgcc ttgcgactaa cgttgtctgg ctctacggat taagatttct tcggaaagaa
     4561 taatggtgta gtgcggactt gtttagcaag tccgtttcgt ttttctcgaa aaatataaaa
     4621 tgaggtgaaa agccatagca aagcaagatt tgttcatcaa tgatgaaatc cgagtgcgtg
     4681 aagttcgtct tatcggtctt gagggtgaac agttggggat caaaccactc aatgaggctc
     4741 aggccattgc tgatagcgcc aacgtggatt tagtattgat tcaaccccaa gctaaaccac
     4801 cagtagcgaa aattatggac tacggtaagt tcaaatttga atatcagaag aaacagaaag
     4861 aacaacgcaa aaaacaaagt gttgtcactg taaaagaagt gcgtctaagt ccagttattg
     4921 ataaagggga tttcgagaca aaacttcgtc atgcccgtaa atttcttgaa aaaggaaata
     4981 aagttaaggt ttcgatccgt ttcaagggtc gtatgattac ccacaaagaa gttggagcga
     5041 aagtattagc tgagttcgct gaagcaaccc aagatattgc cattatcgaa caacgcgcta
     5101 agatggacgg acgtcaaatg tttatgcaat tggccccagc ttcagataaa aaataagctt
     5161 tttaagcaaa aggagaatta acatgccaaa acaaaaaact caccgcgcat cagctaaacg
     5221 tttcaaacgt acaggttctg gtggattgaa acgcttccgc gcttatactt cacaccgttt
     5281 ccacggtaaa actaaaaaac aacgtcgtca ccttcgtaaa gcaggtatgg tacatgctgg
     5341 ggacttcaag cgtatcaaat caatgcttac aggtttgaaa taagccgact tactagaatt
     5401 tagattatca tttggaggaa aattaaatgg cacgtgttaa aggtggcgtt gtttctcgta
     5461 aacgccgtaa acgtatttta aaattagcta aaggttacta cggagctaaa catctcttgt
     5521 tccgcactgc gaaagaacaa gtcatgaact cttactacta tgcataccgt gaccgccgtc
     5581 agaaaaaacg tgatttccgt aaattgtgga tcactcgtat caatgcggct gctcgtatga
     5641 acggtttgtc atactcacaa ttgatgcacg gtttgaaatt ggccgaaatc gaagtaaacc
     5701 gcaaaatgct tgctgacttg gcagtgaacg atgcagcagc tttcacagct cttgcagatg
     5761 ctgctaaagc aaaacttgct aaataagtat aaaaatgtaa agaacttggt agtgatgaac
     5821 caggttcttt atttttggaa agtagctcac tacttgctaa atttcgatat gagtgatata
     5881 cttaactagt aaataaatcg acggttttaa ctttatcgtc atttagtttt gcttttgtta
     5941 cttcatcaac tcgccttgcc taacttcagt tatgcctgcg gctcattgcc tcgtactaa
//
LOCUS       DBNFCK010000109         5850 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.109, whole genome shotgun sequence.
ACCESSION   DBNFCK010000109 DBNFCK010000000
VERSION     DBNFCK010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5850
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.109"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            239..688
                     /locus_tag="AC1PIW_001618"
     CDS             239..688
                     /locus_tag="AC1PIW_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIP6606527.1"
                     /translation="MRTVEQSLNENKHALHSLVVFRRAANTIAKSELETIKKYGLTVC
                     QFGVMEALYNKGNLRIQDLIDKLLSTSGNMTVVIKNMIRDGYIYKTIDTNDRRASLIA
                     LTPLGRETIEKILPEHYDHVGEIFSVLSSEEQDQLAEILKKFKNQQT"
     gene            751..1296
                     /locus_tag="AC1PIW_001619"
     CDS             751..1296
                     /locus_tag="AC1PIW_001619"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase [NAD(P)H] activity
                     [Evidence IEA];
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HIP6606528.1"
                     /translation="MANVLFLVGSLRDGSFNHQMAKKAEAFLTDKAQVSYIDLAKIPL
                     FNQDIETPILPEIAELRAQVDAADAIWIFSPVYNYAIPGIVKNALDWLSRSLDLSNPK
                     GPSILQDKITTVSAVANSGHDHLFKDFQHLLPFIRTQIAGEFTGSTINPEAWGTGVLE
                     LSDETLTKLEQQVADLLAAIQ"
     gene            complement(1396..2508)
                     /gene="glf"
                     /locus_tag="AC1PIW_001620"
     CDS             complement(1396..2508)
                     /gene="glf"
                     /locus_tag="AC1PIW_001620"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HIP6606529.1"
                     /translation="MKKYDYLVVGAGLFGAVFAHEAAKKGKKIKVIEKRDHIAGNIYT
                     KEVEGIQVHEYGAHIFHTSEKEIWDYVNQFAEFNSYTNTPVANYKGEIYNLPFNMNTF
                     NKLWGVVTPAEAGAKIAEQRAVLGGKTPENLEEQAISLVGTDIYEKLIKSYTEKQWEK
                     PCTELPAFIIRRLPVRLTYDNNYFNDTYQGIPIGGYTQIVEKMLEHENIDVETNVDFF
                     ANKEDYLATYPKIVFTGMIDEFFDYQLGELEYRSLRFETEVLDMENYQGNAVVNYTDS
                     ETPYTRIIEHKHFEFGTQPKTIITREYSKTWKRGDEPYYPVNNNRNNHLYTAYKKLSE
                     QQENVIFGGRLGHYRYYDMHQVIGAALQCVRNEVGE"
     gene            2734..2987
                     /locus_tag="AC1PIW_001621"
                     /pseudo
     CDS             2734..2987
                     /locus_tag="AC1PIW_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558050.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(2922..4853)
                     /locus_tag="AC1PIW_001622"
     CDS             complement(2922..4853)
                     /locus_tag="AC1PIW_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000740018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIP6606530.1"
                     /translation="MKTNNVEIIKADSLVRRRGVKVERHLKRVAAYCRVSSDSEDQKN
                     SYESQVRHYKDYISQRSDWELAGIYADEGISGTQVGKRQDFQRLINDCVNGEIDYIVT
                     KAIARFARNTLDTLKYVRMLKDMQIGVYFEEENIDTLTMDGELLLTILSSVAQQEVEN
                     TSAHVKKGLKMKMQRGELIGFQGCLGYDYDVETKQLSINKKEAKIVRYIFERYLEGIG
                     GKVIARELDELGYKSPRGLDHWNDTTVLGIIKNEKYKGDILMGKTFTVDPISKRRLSN
                     FGEEDKYYIKDNHEPIISKEDFEKAQEIRLRRAGNKKTVANVNGKRERYSKMYAFSSM
                     LECGFCGSILSRRSWHCRSDYRKVVWHCVTSIKKGKKFCKHSKGLEELAIEGAFMEAY
                     RQLYHSNENLMTDLLETIESELNDNSLNKELKRITNKLRTLLKKEENLVNLRLEGKIS
                     DTIYNEKYNEISSEKEFLAEEKVNIETTLKSEIDVKKRLTEFKHLLSSQKMLTEFDRA
                     VFESIVEKIIVGGVNSDGEIDPAMLTIIFKTGETQNKDGKQFKSKRKNAKLETDKLCP
                     QNSDEDKKLYSQGTDYTHRSCGIIRKTQLDCPVCSPVFWQAASKDPRTFELSNTHHVD
                     SCHTYTKIKCQQALSRVIA"
     gene            complement(5358..5762)
                     /locus_tag="AC1PIW_001623"
     CDS             complement(5358..5762)
                     /locus_tag="AC1PIW_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIP6606531.1"
                     /translation="MKYYIHVRGSKIPVTETVYKSYWQLVNHEKYLNRKDRKFGVLPF
                     SSFEVDGLLLANVLPDTTVDVERFIENKLLIEQLNEALLTLSDKEFKIIDALYFREHT
                     IREVAIEHEMSATSLFRMRNTILDNLRKFFEK"
BASE COUNT         1692 a         1241 c          904 g         2013 t
ORIGIN      
        1 ttccttttcc ttcacacaaa gaaaaggata taaaaaccta cattcacagt tttgcacttc
       61 catctaagac tatttctcaa atgctcattg ctcgtttttt ccagtacagt tagtattacc
      121 tcaaaaaacg aacttgatta actaaaaact atcttttcca taaaagtact ttacctatga
      181 atataggatt taactttttt tgcttgataa ccgaacgatt tattgctaaa atattctcat
      241 gagaactgtt gaacaatctt taaacgagaa caagcatgca cttcacagtc tagtcgtctt
      301 tcgcagagca gctaatacta tcgcaaaatc agaattagaa acaatcaaaa aatacggact
      361 gacagtttgt cagtttggag tgatggaggc actctacaac aaaggaaatc tgcggattca
      421 agacttaatt gataaattat taagcacttc tggcaacatg actgtcgtta taaagaacat
      481 gattcgagat ggttatatct acaagactat cgatacaaat gaccgtcgtg cttccttgat
      541 tgctctcact ccacttggac gcgaaaccat tgaaaaaatc cttccagagc attatgatca
      601 cgttggagag attttttctg tccttagttc agaagaacaa gaccaattag ccgagatact
      661 taaaaaattc aagaatcagc agacctagga ttgtcaacaa aagacaaata cgatatacta
      721 aatttatgtt tttatgaaaa ggaggccatc atggcaaacg tattgttttt agttggttca
      781 ctacgtgacg gttcttttaa ccatcaaatg gcaaaaaaag ctgaagcatt cttgactgat
      841 aaggctcaag tatcttacat tgatcttgca aaaattcccc tattcaacca agacatcgag
      901 acacctatcc ttcctgaaat tgcagagcta cgtgcacaag tagacgctgc ggatgctatc
      961 tggattttct caccagtata taactatgca atcccaggca tcgtaaaaaa tgctctcgat
     1021 tggctcagtc gttcgctcga tttatccaat ccaaaaggac catctatcct acaagataaa
     1081 atcacaactg tctctgcagt cgcaaacagt ggtcacgacc acctattcaa agacttccaa
     1141 caccttcttc catttattcg cactcagatt gctggtgaat ttacaggttc aaccatcaat
     1201 ccagaagcat ggggaacagg tgtattggag ttatctgacg aaactctaac aaaactcgaa
     1261 caacaagtag ctgacctgtt ggcagcaatt caataataat caaaaaaaac cagaatttct
     1321 ggtttttttg attaacattt taagagcatt ccgacttctg ccaagaataa ttactacttt
     1381 gatgacttct caaaatcatt ccccaacttc atttctcaca cactgtaagg cagctccgat
     1441 gacttggtgc atatcgtagt aacgataatg accaagacgc ccaccgaata tgacattctc
     1501 ttgttgttct gacaatttct tatatgctgt gtatagatgg ttattgcgat tattgttgac
     1561 tggatagtat ggctcatcgc ctcgtttcca ggtcttcgaa tactcacgtg taataatggt
     1621 tttcggttgt gtaccaaatt cgaagtgttt gtgttcgatg attcgagtat atggtgtctc
     1681 gctatctgta tagttgacaa ccgcattccc ttgatagttt tccatatcca acacttctgt
     1741 ctcaaatcga aggctacggt actccaattc accaagttgg taatcaaaga actcgtcaat
     1801 cattcctgta aagacaatct ttggataggt agctaaatag tcttccttat tggcaaagaa
     1861 atcaacattt gtctccacat cgatattttc atgttccaac attttttcta caatctgcgt
     1921 gtaaccgccg attggaatac cttgataagt atcgttaaag tagttattat cataagtcaa
     1981 acgcacgggc aaacgacgga tgatgaaggc tggtaactcc gtacatggtt tttcccattg
     2041 tttttctgtg taggacttga tcaatttttc atagatgtct gtaccaacaa gtgagatggc
     2101 ttgctcttcc aagttttcag gcgttttacc acccaacaca gctcgttgct ctgcaatctt
     2161 cgctcctgct tcagcaggtg tcaccacgcc ccacagttta ttgaaagtat tcatgttgaa
     2221 gggaagattg taaatctcac ctttgtagtt ggcgactggt gtgtttgtat agctgttgaa
     2281 ctccgcaaac tgattcacat aatcccaaat ttctttttca gaagtatgga aaatatgtgc
     2341 gccatattca tggacttgaa taccttctac ttcttttgta tagatgttac ccgcaatgtg
     2401 gtctcgtttt tcgataacct ttattttttt acccttttta gctgcctcat gagcaaagac
     2461 cgctccgaac agacccgctc cgacaactag ataatcgtat tttttcatat tgtttttaca
     2521 tttaactttc taaggaagtc atttctccaa atcttttact agactatcat taataccatc
     2581 gtctcataac taataaagaa tgagttgtga taatctcttc cctaaaacct ttcacttaaa
     2641 gcttgcaata tccaatatta taacagacaa tacaaccttc atcaagttta gcaatagact
     2701 tgttcggtaa ctcaaaggcg gtatactaaa ggtatggaaa caacatacga aaaagtctta
     2761 aaattaaact cagagaattt caaattgttg ataggtgtaa aaaagcgacg tttcatctga
     2821 tgcttgattg tctcactgac gcttatcaag agcaacaccg aaaaggagga cgcccacgcc
     2881 gtctaagtat ggaagagcag cttatcatga ccttgcgcta cttacgctat tacccgactc
     2941 aacgcttgtt ggcatttgat tttggtgtag gtgtggcaac tgtcaacatg atgcgtattt
     3001 gagagttcaa aggtcctggg gtcctttgaa gctgcttgcc aaaaaacagg agagcatact
     3061 ggacaatcta attgcgtttt tctaataatc ccacaacttc tatgtgtgta gtctgttcct
     3121 tgagaataca attttttatc ctcgtcactg ttttgaggac acaatttatc tgtttctagt
     3181 ttagcatttt tacgtttact tttaaattgt ttaccatcct tgttttgagt ttcacctgtt
     3241 ttgaatatta tggttaacat tgcaggatca atttctccgt cgctattaac tccacctaca
     3301 ataattttct caacaatact ttcaaaaact gcacgatcaa actccgtaag cattttctgt
     3361 gaagatagta agtgcttaaa ctcagttagt cttttcttta catcgatttc tgattttaag
     3421 gttgtttcaa tatttacctt ctcttctgct agaaactctt tttctgagga aatttcattg
     3481 tacttttcat tgtatatggt atcgctaatt ttcccttcga gccttagatt tacaagattt
     3541 tcctctttct ttagtaatgt tcgaagtttg tttgtaatcc tttttaattc cttgttaagg
     3601 ctattgtcat tcaattcaga ttcaatcgtt tcaagtaagt ctgtcattaa gttttcattt
     3661 gaatgataaa gttgtctgta agcttccatg aaagccccct caatagcgag ctcttctaat
     3721 cctttactat gcttacaaaa tttctttcct ttctttatcg atgtaacaca gtgccacaca
     3781 accttgcgat aatctgaacg gcagtgccaa cttcttctag ataatattga accgcaaaaa
     3841 ccacattcta acatgctact aaaagcatac atttttgaat agcgttcacg ttttccattt
     3901 acattcgcaa ctgtcttttt atttcctgcc ctacgcaacc taatctcctg agctttctca
     3961 aagtcttctt tagatattat aggttcatga ttatctttga tgtagtactt atcttcttca
     4021 ccaaaattac ttaaacgtct cttgcttatt ggatccactg taaaagtttt gcccattaaa
     4081 atatcgcctt tatatttttc attctttatg attcctaaga ctgttgtatc attccaatga
     4141 tctaatcctc tcggcgattt gtaacctagt tcatcaagtt cccttgcaat tacttttcct
     4201 ccaatacctt ctaagtacct ttcaaagata tagcgaacta ttttggcttc ttttttattg
     4261 attgaaagtt gcttggtttc tacatcatag tcatacccca agcatccttg aaaccctatc
     4321 aattcaccac gttgcatctt catttttaat ccctttttca catgtgcaga tgtattttct
     4381 acttcttgct gtgcaacgga gcttagaata gtcaaaagca attctccatc cattgttagg
     4441 gtatctatgt tttcttcctc aaaatatact ccaatttgca tatcttttaa cattctgaca
     4501 tatttcaaag tatctagggt attcctggca aatctagcaa ttgcctttgt gacaatgtaa
     4561 tctatttctc catttacaca atcatttatt aatcgttgaa aatcttgtcg ctttcctact
     4621 tgtgttcctg aaatcccctc atctgcatag atacctgcca gttcccaatc cgaccgctga
     4681 gagatataat ctttataatg tctcacctga gattcatagc tgttcttctg atcttcgcta
     4741 tcagaactta ctcggcaata ggcagcaact ctttttaaat gtctttctac cttgacacct
     4801 ctacgtcgaa ctaaagaatc agcttttatt atttcaacat tatttgtttt catactgaaa
     4861 cccctccttt tgattctata ttaccgcgtt ctaataaaaa ggtcagctat tttgtgtact
     4921 caatacgcag tctgtttctg attttttcat attcattttt agataacaat tctttagagt
     4981 ataaaaaatt aagcattgat aactgcagac tatattttac aactgtttgt tccaaaattt
     5041 ttcctcctga taacgtgata ctcaattatt tcctccgtgg gtatgattgg ttaaatcact
     5101 catgggcatc tcacccgctg tcctttctcg atggcagcct gaacgttcag aagtatcatt
     5161 atcctcattt gtctcatggc agataggtaa ccaacctatt ttatagccga taattctcgc
     5221 tcatctcaca gcgtaccagg ctaaattgct ccacaaatga ttaaagtccc acttcagtct
     5281 attcagttgt caaagggcaa aaagagacta aaaattcctc tcactataaa gagtgaattt
     5341 tttagtctcc tgtttcctta tttttcaaaa aattttcgta ggttgtccaa aattgtattt
     5401 ctcatccgaa atagactagt cgcagacatc tcatgctcta ttgctacttc tctgatggta
     5461 tgctctctaa aatacagagc atcaatgatt ttaaactctt tatcagataa ggttaataaa
     5521 gcctcattta gttgctcaat aaggagcttg ttttctataa aacgttcaac atcaacagtt
     5581 gtatcaggca gtacatttgc tagcagtaac ccatctactt caaatgatga aaaaggtaat
     5641 actccaaatt tcctatcttt tctattcaga tacttctcgt gattgaccaa ttgccagtag
     5701 cttttataaa ccgtttctgt tacggggatt ttactacctc taacgtgaat ataatatttc
     5761 atttcagcct ccgtttatga ttttttctta gtcataaaag gagggctcct tatctcaacc
     5821 atagctattt ctcactttta tcaaatcaag
//
LOCUS       DBNFCK010000110         5844 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.110, whole genome shotgun sequence.
ACCESSION   DBNFCK010000110 DBNFCK010000000
VERSION     DBNFCK010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5844
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.110"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..1041
                     /gene="dgt"
                     /locus_tag="AC1PIW_001624"
     CDS             <1..1041
                     /gene="dgt"
                     /locus_tag="AC1PIW_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTP triphosphohydrolase"
                     /protein_id="HIP6606532.1"
                     /translation="MSVVVETGALVHDIGNPPFGHHGERILNDLAKDIGFEGNAQGLR
                     VLKNIEKKSPDHKGLNLTYRTLASILKYYVPYRKSRKTEKFIYKEDYEEFKKRFKEMD
                     VFVRTIDVQIVDLADEIAYCAHDLEDALASNYFTIDQFVFLLEQKLGEDQTLEDFKEW
                     IEEAKNKAKRSKSNDDYDFYFRRELLSIIVNKFIEDIDIVELNEKDKKKLGTDQDQEL
                     GLGHYKKLAGALKKTIFEGVTNSDDIILYENVGTKVLTRLFALFMNEEYNRESFLMPI
                     EYRFEKDDTQSDKKRKVLDYISGMMDTYAIEMYIKHFGTDPFLEKYDAAIFKKGTGKK
                     LIECLAQNFLHK"
     gene            complement(1100..2098)
                     /locus_tag="AC1PIW_001625"
     CDS             complement(1100..2098)
                     /locus_tag="AC1PIW_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIP6606533.1"
                     /translation="MVTIKDIAKQAHLSSATVSRVLKGDLTLSVSQETRDRIFNLAQE
                     LGYTKHIKKQAPQQKGTIGIVQWYTESEELADLYYYSIRASIEHTASLLGYQIVRSFN
                     DLTNPLLQEVDGIIAVGKFSSGQIAELSSLSSKIIFVDSDTLTEGFSCVTTDFEHSVQ
                     TVIDHFHSQGLTDIGLLVGQEETTDGHQLPTDPRLTAFRDYLDTLGMLQEDYIYQGKF
                     STQSGYELMSQAIEDLGDKLPPAFFMANDTLAVGALRALQERQIAVPDRIQLITFNDT
                     AITRQVYPALSSISVFTEEMGQEAMQLLDRIITSPTPHHPRKIKLGTQLVIRESSY"
     gene            2232..3404
                     /locus_tag="AC1PIW_001626"
     CDS             2232..3404
                     /locus_tag="AC1PIW_001626"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HIP6606534.1"
                     /translation="MNTEQLKQAFLDVFGQEADATFFSPGRINLIGEHTDYNGGHVFP
                     AAITLGTYGAARKRDDQVLRFYSANFEELGIIEVDLNNLVFDKADNWTNYAKGVLKFL
                     KEAGHVIDTGMEVFVYGNIPNGSGLSSSASLELLIGIIAEELYGLELTRLDLVKIGKQ
                     TENHFIGVNSGIMDQFAIGMGADNRAIYLDTNSLEYELVPLDLGDHVIVIMNTNKRRE
                     LADSKYNERRAECEKAVEELNAVLNIQTLGELDEWTFDQYSYLIKDENRIKRARHAVL
                     ENQRTLQARKALEEGDLATFGRLVNASHVSLEHDYEVTGLELDTLAHTAWEQEGVLGA
                     RMTGAGFGGCGIAIVHKDKVEAFKENVGKTYTEVVGYAPSFYVAEIAGGSRVLSRK"
     gene            3414..4895
                     /gene="galT"
                     /locus_tag="AC1PIW_001627"
     CDS             3414..4895
                     /gene="galT"
                     /locus_tag="AC1PIW_001627"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HIP6606535.1"
                     /translation="MMTGLVDRFVEQVIANSDFEEMDAIYLRNRVLALVGDQVLTVQT
                     DLEDLIELKDELLAHGVRTGFVGELLEEQDMVGSCLMDLMTPSPSQVNRDFWQTYQDS
                     PEQAISDFYELSKRNDYIKMAAIAKNIYYPVSTEYGDLEITINLSKPEKDPKSIAAAT
                     KAEASNYPKCLLCMENEGYQGRINHPARANHRIIRLELGQEKWGFQYSPYAYYNEHAI
                     FLNQEHVPMVISPRTFEQLLDLLDLLPGYFVGSNSDLPISGGSILTHNHYQGGRHSFA
                     MDQAPIEHQLVFEGFESVSAGIVKWPMSVIRLSSADKPSLLGLAAKILEKWRSYSDDS
                     VQIKAETDGTPHHTITPIARKRGDLYELDLVLRDNQTSEEFPDGIYHPHPDVQHIKKE
                     NIGLIEVMGLAILPPRLKAELAEVEKFLLGQDSQVADYHQPWAESLKTAHPDVTEETV
                     EQVVRESVGQIFARVLEDAGVYKRTPEGQAAFLRFVEFVGLAT"
     gene            4971..>5844
                     /locus_tag="AC1PIW_001628"
     CDS             4971..>5844
                     /locus_tag="AC1PIW_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIP6606536.1"
                     /translation="MAEKRLGTLITVIAGIAWGLSGVSGQYLMSRGVSVDMITSLRLL
                     VSGLFLVGLAYATAKEQLLAVLKDKNALLGIFIFAMLGLVLNQTAYLQAIYHTNAGTA
                     TVLQYLCPILVLAYTCLKNREKPSGIELISIILAIAGTFLIATHGKLDELAVTPIGLF
                     WGIFSAFTYALYIILPGKLIRQYGSITVIGLGMLMGGFVVTLGLQTWQHRLPIDGGSF
                     LGLLGIVGVGTIFAYTAFLKGVSMVGPVNGSLLASIEPIASVFFAVWLVNEQFYAIDF
                     VGMLLILLAVLLISL"
BASE COUNT         1625 a         1013 c         1481 g         1725 t
ORIGIN      
        1 atgtctgtcg ttgttgaaac tggtgcactt gtacacgaca ttggcaatcc cccatttggt
       61 catcatggag aaagaatttt aaatgatttg gcaaaggata ttggttttga aggaaatgcc
      121 caagggttac gggtattaaa aaatattgaa aaaaagtctc cagatcataa aggcttaaac
      181 ttaacatata gaaccctagc ttcaattcta aaatattatg ttccatatag aaaatcacga
      241 aagactgaga agttcatata caaagaagat tatgaggaat ttaaaaagag atttaaagaa
      301 atggatgtgt ttgtgagaac aattgatgtt caaattgttg atttggcaga tgaaattgct
      361 tattgtgcac atgacttgga agatgcattg gcgagtaact acttcaccat tgatcaattt
      421 gtatttttgt tggaacagaa attgggagaa gatcaaacat tagaggattt taaggaatgg
      481 attgaagagg caaaaaataa ggcaaaaaga agtaaatcca atgatgatta tgatttttat
      541 tttagacgag aattgctttc tattattgtg aataaattca ttgaggatat agacattgta
      601 gaacttaatg aaaaagacaa gaaaaaatta ggaactgatc aggatcaaga actagggctc
      661 ggtcattata aaaaacttgc tggtgcattg aagaaaacaa ttttcgaagg cgtaacaaat
      721 tctgacgata ttattttata tgaaaatgtt gggacgaaag tgttgactag attatttgct
      781 ttattcatga atgaagaata taatcgggag agttttttga tgccgatcga atatcgattt
      841 gaaaaagacg atacccaaag tgacaagaaa agaaaagtac tagattatat ttctggcatg
      901 atggacactt acgcaattga gatgtatata aaacattttg gtacagatcc gttcttagag
      961 aaatatgatg cagctatatt caagaaagga actggtaaaa agctaataga atgtttagca
     1021 caaaatttct tacataaata gccatatgtt taagaaaacc gagagttcgt gttgaattct
     1081 cgggttttct tatgcacttt tagtaagaac tctcgcgtat taccagctgg gtacccaact
     1141 taatcttacg ggggtggtgg ggcgttggac ttgttatgat acggtccagt agctgcatag
     1201 cttcctggcc catttcctca gtgaagacgc tgatggaaga cagggctgga tagacttggc
     1261 gcgtgatggc tgtatcgtta aaggtgatga gttggattct atctggcact gcgatttgac
     1321 gttcttgtaa agcacgcagg gcaccgacag ccagagtatc gtttgccatg aaaaaggcag
     1381 ggggtagctt gtcgcctagg tcctcaatag cctggctcat cagctcatat cccgactggg
     1441 tggagaattt tccttgatag atgtagtctt cttggagcat gcccaaggtg tctaggtagt
     1501 cacgaaaggc agtcaggcgg gggtctgttg gcagttggtg tccatctgtg gtttcttcct
     1561 gtccgactag gagtccaata tcagtcagac cttgtgaatg gaagtggtcg ataactgtct
     1621 gaacagagtg ttcaaagtca gttgtgacgc aggagaatcc ctcggtcagc gtatcagagt
     1681 ccacaaaaat gattttagaa gacaggctag atagctctgc tatttgtcca gaagaaaatt
     1741 tgccaactgc gatgatgccg tccacttctt ggaggagggg attggtcagg tcgttgaagg
     1801 agcggacgat ttggtagccg agtaagctgg cggtatgctc aatactggcc cgaatggagt
     1861 agtagtagag gtcggccagc tcttcgctct cggtgtacca ctgaacaata ccgatggtgc
     1921 ctttttgttg aggtgcttgt tttttgatgt gcttggtgta gcccaattcc tgtgccaagt
     1981 tgaaaatgcg gtcgcgggtt tcttggctga cggatagggt caggtctccc ttgagaacgc
     2041 gggaaacggt ggcagaggaa aggtgggctt gttttgctat gtctttgatt gtaaccatgt
     2101 tcttctcctg tttatttgtc tctagtatag cataaaaaaa caaaattagt aaaaatttag
     2161 taaaaatatt gacttttaaa ttgttgagtt gtacaataaa agaaaacgat tacaaaatct
     2221 aggaggttct tatgaacaca gaacaactca agcaggcctt tctcgatgtg tttggtcaag
     2281 aggcagatgc gactttcttc tcaccaggtc ggattaattt gattggtgag cacacggact
     2341 acaatggtgg tcatgtcttt ccagcggcta ttaccttggg gacctacggg gctgctcgca
     2401 aacgtgatga ccaagttttg cgtttctatt ccgcaaactt tgaagaactt ggaattatcg
     2461 aagtggattt gaacaatctg gtctttgata aggcggataa ctggaccaac tatgccaagg
     2521 gggttctcaa attcttgaaa gaagcagggc atgtcattga tacagggatg gaagtctttg
     2581 tttacggtaa cattccaaac ggttcaggct tgtcatcatc agcttccttg gaactcttga
     2641 ttggcattat cgcagaagag ttgtatggac ttgaattgac tcgacttgat ttggtgaaaa
     2701 ttgggaaaca aacggaaaat cactttattg gtgtcaattc tgggatcatg gaccagtttg
     2761 cgatcggtat gggagcagat aatcgggcga tttatctaga taccaatagc ttggagtatg
     2821 aattggtacc gctagatttg ggtgaccatg tgattgtgat catgaacacc aacaaacgcc
     2881 gtgaattggc agattctaag tacaatgaac gtcgcgcaga atgtgaaaaa gcagtggaag
     2941 aattgaatgc tgtcctgaac attcaaactc tgggagaatt ggatgaatgg acctttgatc
     3001 aatatagtta tttgattaag gatgaaaatc gtatcaagcg tgcccgccat gctgttttgg
     3061 aaaatcaacg gaccttgcag gctcgtaagg ccttggaaga aggagatttg gctacctttg
     3121 gtcgtctggt caatgcttcc catgtttcct tggaacatga ttatgaagtg acaggtttgg
     3181 aattggatac cttggctcac acagcttggg agcaagaagg agttcttggt gctcggatga
     3241 caggagctgg cttcggtggc tgtggtattg ccattgtcca caaggataag gtggaagcct
     3301 tcaaagaaaa tgtcggcaag acctatacag aggttgtggg ctatgcacca agcttctatg
     3361 tagcggagat tgctggaggc tctcgcgttt tatctcgaaa atagattgga gaaatgatga
     3421 caggattggt ggatcgtttt gttgagcagg ttattgccaa cagtgatttt gaggaaatgg
     3481 atgctatcta cctgcgaaat cgggtcttgg ctttggtggg agaccaggtt ctgactgttc
     3541 aaacagattt agaggacttg attgaattaa aagatgaact attggcacat ggagttcgaa
     3601 ctggttttgt gggtgaattg ctggaagagc aggacatggt tgggtcttgt ttgatggatt
     3661 tgatgacacc gagtccgagt caggtcaatc gtgatttttg gcagacctat caggatagtc
     3721 cagagcaagc gattagtgac ttttatgaat tgagcaaacg taatgactat atcaagatgg
     3781 ctgcgattgc gaaaaatatc tattatccag tttcgacaga gtatggggat ttggaaatca
     3841 cgatcaatct gtccaaacct gagaaggatc ccaagtctat tgcagctgct acgaaagctg
     3901 aagcaagcaa ttatcccaaa tgcctactct gcatggaaaa tgagggctat cagggacgga
     3961 ttaatcatcc tgcacgcgcc aaccaccgca ttattcgctt ggagcttgga caggaaaagt
     4021 ggggcttcca gtattcgccc tatgcttact acaatgaaca tgctattttc ctaaatcagg
     4081 aacatgtgcc gatggttatt agtccgcgaa cttttgagca gctcttggac ttgttggatt
     4141 tgttgccagg ttattttgtc ggttccaact ctgacctccc tatctcgggt ggctctatct
     4201 tgacccacaa tcactatcag ggtgggcggc acagttttgc catggatcag gcgccgatag
     4261 aacaccagct ggtcttcgaa ggctttgagt cagtttcggc tggcattgtc aaatggccca
     4321 tgtcggtgat tcgcttgagt tcagcagaca aaccgtcgct tcttggttta gcggctaaga
     4381 ttttggaaaa atggcggagc tactcagatg atagcgttca gattaaggct gagacagatg
     4441 gaacacccca tcatacgatt accccaattg ctcggaaacg aggcgatttg tacgagctgg
     4501 atttggttct ccgcgataat caaacttcag aagagtttcc agatggcatc tatcatccac
     4561 atccagatgt gcaacatatc aagaaagaaa atatcggctt gattgaggta atgggcttgg
     4621 cgattttacc cccacgcttg aaggcagaat tggctgaagt agaaaaattc ttactgggtc
     4681 aagatagtca ggtggctgac tatcatcagc cttgggcgga aagcctgaaa acagctcacc
     4741 cagatgtgac ggaagaaact gtcgaacaag tagttcgtga gtcagtggga caaatctttg
     4801 cgcgtgtatt ggaagatgca ggggtctaca aacgtactcc agaaggacag gcagcctttc
     4861 tacgattcgt agaatttgta ggtttagcaa cataaatgta agaaagagtg ggacggtgtc
     4921 ctgctctttt tggtataatg gaagtattca cagtgtgaga aggagatatg atggcggaaa
     4981 agagattggg gacactgatt acagtaatag caggtatagc ctggggcttg tctggagtga
     5041 gtggacagta cttgatgtct cgtggtgttt cagtggatat gattacctcc ctgcgtttgt
     5101 tggtgtcggg actctttctc gttggcctgg cttatgctac agcgaaagaa cagctattgg
     5161 cagttttgaa agataaaaat gccttgctag gaatttttat ctttgccatg cttggcttag
     5221 ttcttaatca gacagcctat ttacaagcca tctatcatac caatgcggga acggctactg
     5281 ttctacaata tctctgtcct attttggttt tggcatatac ctgtttgaaa aatagggaaa
     5341 aaccatcagg gattgagttg atttcaatta tcttagctat agcaggcact tttttgattg
     5401 cgactcatgg caaattagat gaattggcag tgactccaat cggtttgttc tggggaatct
     5461 tctcagcctt tacttatgcg ctatatatta ttttgccagg taaattgatt cgtcaatacg
     5521 gcagtataac ggtgattggt ttagggatgt taatgggtgg ttttgtagtc acgcttggtt
     5581 tgcaaacttg gcagcatcgt cttcctattg atggtggaag tttcctgggc ctgttaggta
     5641 ttgtcggagt cgggacgatt tttgcctata ctgcctttct taaaggcgtc agtatggttg
     5701 gtcctgtcaa tggcagtctt ttggcctcga ttgagccgat tgcatctgtc ttttttgcag
     5761 tatggttggt caatgaacaa ttttatgcaa ttgattttgt tggaatgttg ttgattttac
     5821 ttgctgtttt gcttatttct ttaa
//
LOCUS       DBNFCK010000111         5723 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.111, whole genome shotgun sequence.
ACCESSION   DBNFCK010000111 DBNFCK010000000
VERSION     DBNFCK010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.111"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            411..1427
                     /gene="neuB"
                     /locus_tag="AC1PIW_001629"
     CDS             411..1427
                     /gene="neuB"
                     /locus_tag="AC1PIW_001629"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HIP6606537.1"
                     /translation="MVYIIAEIGCNHNGDVHLARKMVEVAVDCGVDAVKFQTFKADLL
                     ISKYAPKAEYQKITTGESDSQLEMTRRLELSFEEYLDLRDYCLEKGVDVFSTPFDEES
                     LDFLISTDMPVYKIPSGEITNLPYLEKIGRQAKKVILSTGMAVMDEIHQAVKILQENG
                     TTDISILHCTTEYPTPYPALNLNVLHTLKKEFPNLTIGYSDHSVGSEVPIAAAAMGAE
                     LIEKHFTLDNEMEGPDHKASATPDILAALVKGVRIVEQSLGKFEKEPEEVEVRNKIVA
                     RKSIVAKKAIAKGEVFTEENITVKRPGNGISPMEWYKVLGQVSEQDFEEDQNICHSAF
                     ENQM"
     gene            1439..2572
                     /gene="neuC"
                     /locus_tag="AC1PIW_001630"
     CDS             1439..2572
                     /gene="neuC"
                     /locus_tag="AC1PIW_001630"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HIP6606538.1"
                     /translation="MKKICFVTGSRAEYGIMRRLLSYLQDDPEMELDLVVTAMHLEEK
                     YGMTVKDIEADKRRIVKRIPLHLTDTSKQTIVKSLATLTEQLTVLFEEVQYDLVLILG
                     DRYEMLPVANAALLYNIPICHIHGGEKTMGNFDESIRHAITKMSHLHLTSTDEFRNRV
                     IQLGENPNHVLNIGAMGVENVLKQDFLTREELAMELGIDFAEDYYVVLFHPVTLEDNT
                     AEEQTQALLDALKEDGSQCLIIGSNSDTHADKIMELMHEFVKQDSDSYIFTSLPTRYY
                     HSLVKHSQGLIGNSSSGLIEVPSLQVPTLNIGNRQFGRLSGPSVVHVGTSKEAIVGGL
                     GQLRDVIDFTNPFEQPDSALQGYRAIKEFLSVQASTMKEFYDR"
     gene            2562..3212
                     /locus_tag="AC1PIW_001631"
     CDS             2562..3212
                     /locus_tag="AC1PIW_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HIP6606539.1"
                     /translation="MIDRGESLMKKVAFLGAGTFSDGVLPWLDRSRYELIGYFEDKPI
                     SDYRGYPVFGPLQDVLTYLDDGKVDAVFVTIGDNVKRKEIFDLLAKDHYDALFNIISE
                     QANIFSPDSIKGRGVFIGFSSFVGADSYVYDNCIINTGAIVEHHTTVEAHCNITPGVT
                     INGLCRIGEGTYIGSGSTVIQCIEIAPYTTLGAGTVVLKSLTESGTYVGVPARKIK"
     gene            3221..4453
                     /locus_tag="AC1PIW_001632"
     CDS             3221..4453
                     /locus_tag="AC1PIW_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylyltransferase domain-containing protein"
                     /protein_id="HIP6606540.1"
                     /translation="MEPICLIPARSGSKGLPNKNMLFLDGVPMIFHTIRAAIESGCFK
                     KENIYVSTDSEVYKEICETTGVQVLMRPADLATDFTTSFQLNEHFLQDFSDDQVFVLL
                     QVTSPLRSGKHVKEAMELYGKGQADHVVSFTKVDKSPTLFSTLDENGFAKDIAGLGGS
                     YRRQDEKTLYYPNGAIYISSKQAYLADKTYFSEKTAAYVMTKEDSIDVDDHFDFTGVI
                     GRIYFDYQRREQQNKPFYKRELKRLCEQRVHDSLVIGDSRLLALLLDGFDNISIGGMT
                     ASTALENQGLFLATPIKKVLLSLGVNDLITDYPLHMIEDTIRQLMESLVSKAEQVFVT
                     TIAYTLFRDSVSNEEIVQLNDVIVQSASELGISVIDLNEVVEKEAMLDYQYTNDGLHF
                     NQIGQERVNQLILTSLTR"
     gene            4650..5523
                     /locus_tag="AC1PIW_001633"
                     /pseudo
     CDS             4650..5523
                     /locus_tag="AC1PIW_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681261.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT         1740 a         1007 c         1281 g         1695 t
ORIGIN      
        1 cccaaatgag aggtgctcat aagaattgac catcactgcc atctacccaa agttcaagta
       61 ttctctacca tgaaaattgt gctataatca agtataaaga agggaatgtt tcttaaagga
      121 cgtatgcgcc tctgcttatg ccagaagtca tgaggtaaat ctccctaaaa attgggtaga
      181 aaagcagatt aaacttccac caatctattg aagatcgtgt gaagaagcag gctttagaag
      241 caacaagccc tgagactatt cgaaagaaat ctagggctat tttttctaat cggctatcag
      301 aagtgaagta gcgatcttta ttagtgttct tttactactt aaggaaaacc aagctgctcc
      361 ctcaagactt tatgggagcg atttacagtc atttttagaa aggaaataaa atggtttata
      421 ttattgcaga aattggttgt aatcacaacg gtgatgttca tctagcacgg aaaatggtag
      481 aagttgccgt tgattgtggt gtggatgccg ttaaatttca gacatttaag gcagatttgt
      541 tgatttcaaa atacgcacca aaggcagaat accaaaaaat tacaacagga gagtcagatt
      601 ctcagctcga aatgactcgt cgtttggaat tgagctttga agagtatctt gatttgcgtg
      661 attactgtct tgaaaaggga gttgatgtgt tttcgacacc ttttgatgag gaatcattgg
      721 acttcttgat tagcacagat atgcccgttt ataagattcc atctggtgag attaccaatc
      781 ttccctattt ggaaaaaatt ggtcgtcaag ctaagaaagt tattctttca actggtatgg
      841 ctgttatgga tgaaattcat caagcggtga agattttgca ggaaaatgga acgaccgata
      901 tttcgatttt gcattgtaca accgagtatc caacccctta ccctgctttg aatttgaatg
      961 tcttgcatac cttgaaaaaa gaatttccaa acttaacaat tggctattca gaccatagtg
     1021 ttggttcaga agtacccatc gctgctgcag caatgggagc tgaattgatt gaaaagcact
     1081 ttactctgga caatgaaatg gaaggaccag atcataaagc gagtgctact cctgatatct
     1141 tagcagcctt ggtaaaagga gtgaggatag tggaacaatc tcttggtaaa tttgaaaaag
     1201 agccagaaga agttgaagta cgaaataaaa ttgtagctag aaaatctatt gttgccaaaa
     1261 aagcaattgc taaaggcgaa gtctttacag aagaaaacat cactgtcaaa agaccaggaa
     1321 atggaatttc gccaatggaa tggtacaaag tcttggggca ggtgagtgag caggattttg
     1381 aggaagacca aaatatttgc catagtgctt ttgaaaatca aatgtaagcg gagtaaggat
     1441 gaaaaaaatt tgttttgtga caggctctcg tgccgaatat gggattatgc gtcgcttatt
     1501 gagctatcta caggatgatc cagaaatgga gctggatctt gtagtgacag ccatgcatct
     1561 agaagaaaaa tatgggatga cggtcaaaga catcgaagcg gacaagcgta ggattgtcaa
     1621 gcggattcca ttgcatttga cggatacgtc taagcagaca atcgtcaaat ctttagcgac
     1681 cttgacagag caactcacgg ttctttttga agaagtccag tatgacttgg tgttgattct
     1741 gggggatcgc tatgagatgc taccagttgc caatgctgcg ttgctttata atattcctat
     1801 ttgccatatt catggtggtg aaaaaaccat gggaaatttt gatgagtcga ttcgccatgc
     1861 cattaccaag atgagtcacc ttcatctgac atcaacggat gaatttagaa atcgtgtcat
     1921 tcaactagga gaaaatccca accatgtact gaacatcgga gctatgggtg ttgaaaatgt
     1981 tttaaaacaa gactttttga caagagaaga gttggcgatg gaacttggaa ttgattttgc
     2041 cgaggattac tatgttgtac tctttcaccc tgttaccttg gaggataaca cagccgaaga
     2101 acaaacgcag gccttattag atgctctaaa agaagatggt agccagtgtt tgataattgg
     2161 atccaattcg gatacacatg ccgataagat aatggaattg atgcatgaat ttgtaaaaca
     2221 agactctgat tcttacatct ttacttcgct tccaactcgt tattaccatt ccttggtcaa
     2281 gcattcacaa ggtttaatag ggaattcttc gtcaggtttg attgaagtgc cctcattaca
     2341 ggttccgacc ttaaatattg gaaatcgcca atttggacgt ttgtcaggac cgagtgtggt
     2401 acatgttgga acttctaagg aagcgattgt tggtggtttg gggcaattac gtgatgtgat
     2461 agattttacc aatccatttg aacaacctga ttctgcttta caaggttatc gagctatcaa
     2521 ggaattttta tctgtacagg cctcaaccat gaaagagttt tatgatagat aggggagaaa
     2581 gtttgatgaa aaaagtagcc tttctaggag cgggtacctt ttcagatggt gtccttcctt
     2641 ggttggatag aagtcgatat gaactcattg gatattttga agataaaccg atcagtgact
     2701 atcgtggcta tcctgtattt ggtcccttgc aagatgtcct aacctatttg gatgatggaa
     2761 aagtagatgc tgtcttcgtc actataggtg acaatgtcaa gcgcaaggaa atctttgact
     2821 tgcttgccaa agatcattat gatgctttgt tcaacatcat tagcgagcaa gccaatattt
     2881 tttccccaga tagtatcaag ggacgagggg ttttcatagg tttttcaagt tttgtaggag
     2941 ccgattccta tgtctatgac aattgtatca tcaatacggg tgccattgtg gaacatcata
     3001 ccacggtgga ggcccattgt aacattactc caggagtgac cataaatggc ttgtgccgta
     3061 tcggagaagg cacttatatt ggaagtggtt caacagtgat tcaatgtatc gagattgcac
     3121 cttatacaac attgggggca gggacagttg ttttgaaatc gttgacggag tcagggacct
     3181 atgttggtgt acctgctaga aagattaaat aggtgaattg atggaaccaa tttgtctgat
     3241 tcctgctcgg tcaggatcaa aaggtttacc aaataaaaac atgttatttt tagatggtgt
     3301 accgatgatt ttccatacca ttcgagctgc gattgagtct ggatgtttta agaaagaaaa
     3361 tatatatgtc agtactgatt cagaggttta caaggaaatt tgtgaaacaa ctggggttca
     3421 agtcctcatg cgtccagctg acttggcgac agattttaca acctcttttc aactgaacga
     3481 acatttttta caagattttt ctgatgacca agtatttgtt ctcctgcaag ttacgtcccc
     3541 attaagatcg ggaaaacatg tcaaggaggc gatggagtta tatgggaaag gtcaagctga
     3601 ccacgttgtt agctttacca aagtcgataa gtctccaaca ttgttttcaa ctttagacga
     3661 aaacggattc gctaaggata ttgcaggatt aggtggcagt tatcgtcgtc aagatgagaa
     3721 aacactctac tatcctaatg gagcgattta tatttcttct aagcaggctt atttagcgga
     3781 taaaacttat ttttctgaaa aaacagcggc ctatgtgatg acgaaggaag attcgattga
     3841 tgtagatgat cactttgatt ttactggtgt tattggtcga atttactttg attaccagcg
     3901 tcgtgagcaa caaaacaaac cattttataa aagagagtta aagcgtttat gtgagcaacg
     3961 agtccatgat agtcttgtga ttggcgatag tcgtctgtta gccttgttac tggatggttt
     4021 cgataatatc agcatcggtg ggatgacagc ttcgacagca cttgaaaacc aaggtctctt
     4081 tttggctact ccgataaaga aagttttgct ttctcttggt gtgaatgatt tgattactga
     4141 ctatcccttg catatgattg aggatactat tcgccagctg atggaaagtc ttgtttccaa
     4201 agcagagcag gtttttgtga cgacgattgc ctacacgctg tttcgtgata gcgtttcaaa
     4261 tgaagaaatt gtgcagctga atgacgttat tgttcagtca gcaagtgaac tgggtatttc
     4321 agtgattgat ctaaatgaag ttgttgaaaa agaggcgatg cttgactatc agtataccaa
     4381 tgatggattg catttcaatc agattggaca agagcgtgtg aatcagctga ttttgacaag
     4441 tttgacaaga taatttggtg atagaagcta tttcagtggc tagactatgt tggtatgtgt
     4501 tttagagcca aggaataaca tctgtagagg atgctagcct tgagaattga caaccattta
     4561 gttgtttaat tatataaggg gacctctaaa aactccctaa atttcccaaa aatgagataa
     4621 tagaataaaa agtaatgagg agagctgtca tgcatttatt cacagacgat gaaaaaatct
     4681 tgtcaaaact atcagagaaa ggcaatccct tagaacgttt ggatgccgtt atggattgga
     4741 atatctttct tccattgttg tcagagttat tcagtcgtaa agataaagtc atcagtcgtg
     4801 gcggtcgtcc tcacctagac tatctcatga tgttcaaagc gctcttgctt caacgtcttc
     4861 ataacctatc tgacgatgcc atggaatatc aactgctgga tcgtatatct tttcgtcgtt
     4921 ttgttggttg tcatgaagac actgttcccg atgcgaaaac tatctggctc tatcgtgaga
     4981 aattaaccaa gtcaggtcgt gaaaaggagt tgttcgattt gttctatgcc catctcacag
     5041 atgaaggggt gattgcccat tcaggtcaga ttgtggatgc tacctttgtc gaatgcccta
     5101 aacaacgcaa ttcacgtgag gacaatcaga aaatcaaaac ttatcgaaaa ttatgaggtc
     5161 acaacagcta gtgtacacga ctccaatgtc ctagctcctc tttgtgatgc caatgaagcg
     5221 gtttttgatg acagtgctta tgttggaaaa tcagtaccag aaggttgtcg ccaccacacg
     5281 attcgtcgtg cttttagaaa taaaccgttg actgagactg ataaggtcat taatcgacat
     5341 attaccaaag tccgttgtcg cgttgagcat ggttttggct tcattgaaac taacatgaaa
     5401 ggtaacatct gtcgagcaat tgggaaggca cgagctgaaa ccaatgtgac cttaaccaac
     5461 ctgctctaca atatctgtcg ttttgagcaa atcaaacgac tgggattacc atcgtgggct
     5521 tagtgcgccc aaaaatagga aaataagcaa aaagaggctg ggcaaaaact agtttctcac
     5581 aataaaaaaa cggctctttg tcaactgtag tgggtagacg aaaagctaac acctagagag
     5641 gacgaaattc gttctctcat ttttgatgtt taaagcgtaa ccgcctaata acaaggtatc
     5701 tatccaatca cacattcctc cat
//
LOCUS       DBNFCK010000112         5460 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.112, whole genome shotgun sequence.
ACCESSION   DBNFCK010000112 DBNFCK010000000
VERSION     DBNFCK010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5460
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.112"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..632)
                     /locus_tag="AC1PIW_001634"
     CDS             complement(<1..632)
                     /locus_tag="AC1PIW_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIP6606541.1"
                     /translation="MISNEQYDYLRAHPTFERLTRDSFDQLAKHIRFRKIPKGQIFFY
                     AEDPRDYLFVLYKGYARIEQYDETDSFTYLDYIRQGGAFPFGDMFQDKPYHYTAIAIT
                     DLEYFIVPMVLFETLSKINPSQMAYICQKLSKVLRFQELRLRNAMRSKASDRVVQALA
                     LLYWDMCQREQFATLPFEIHIQEISRLAATTRETVSHVLKQLKQDGKIAY"
     gene            complement(918..1835)
                     /gene="whiA"
                     /locus_tag="AC1PIW_001635"
     CDS             complement(918..1835)
                     /gene="whiA"
                     /locus_tag="AC1PIW_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HIP6606542.1"
                     /translation="MSFTVQVKEELLLQSSQNKSELSAIIKLSGSLGLASSGLTLSIS
                     TENAKIARHIYELLLHFYQIKAEIRHHQKPNLKKNRVYAVLIEDGVNEILNDLHLADS
                     FFGLETGISPLVLENDSWSQAYLRGAFLAAGSVKDPEKGKYQLEIASVYSDHANDLAN
                     LMQKFLLDAKVIERSKGTITYLQRAEDIMDFLLVIGAEETKTEFENVKLLREARNDLN
                     RATNAEAANIAKTVNASMKTINNIIKIMDTIGLDQLSGDLQEIAQLRIQHPDYSIQQL
                     ADSLTVPITKSGVNHRLRKINKIADELTD"
     gene            complement(1832..2806)
                     /locus_tag="AC1PIW_001636"
     CDS             complement(1832..2806)
                     /locus_tag="AC1PIW_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HIP6606543.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLRDKDVEITAIVTVADDGGSSGE
                     IRQALQVTPPGDLRNVLLAMSDMPKLYEQIFQYRFADSDGPLAGHPLGNLIIAGISEM
                     QGSTYNAMRLLTRFFHTTGRIYPSSEQALTLHAIFTDGTEVAGESKISKHNGMIDHVY
                     VTNSYNDDEPKASRQVVETIMESDMIVLGPGSLFTSILPNLMISDIGKALKETKAEVT
                     YVCNIMTQRGETEFFSDADHVAVLNAHLAEQFIDTVLVNIEPVPQEYMNSNQFDEYLV
                     QVKHDFAGLQKQANRVISSNFLRLENGGAFHDGDLVVEELLKILQVRP"
     gene            complement(2803..3690)
                     /gene="rapZ"
                     /locus_tag="AC1PIW_001637"
     CDS             complement(2803..3690)
                     /gene="rapZ"
                     /locus_tag="AC1PIW_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HIP6606544.1"
                     /translation="MSDKLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPTLLPKF
                     LELIRHSQDNNKIALVVDMRSRSFFSEIREVLDEIEGAEDLDFKVLFLDATDSELVAR
                     YKETRRSHPLAADGRVLDGIQLERELLAPLKNMSQNVIDTTELTPRNLRKVISEQFAS
                     QDNQPSFRIEVMSFGFKYGLPLDADLVFDVRFLPNPYYKLELRNLTGLDAPVFDYVME
                     HQESEEFYSHLLGLIEPILPGYQKEGKSVLTIAVGCTGGQHRSVAFAKRLADDLEKNW
                     NVNRSHRDKDRRKETVNRS"
     gene            complement(3712..4089)
                     /locus_tag="AC1PIW_001638"
     CDS             complement(3712..4089)
                     /locus_tag="AC1PIW_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIP6606545.1"
                     /translation="MKTIHTDKAPAAIGPYVQGKVVGNFLFASGQVPLSPETGEVVGE
                     TIQEQTEQVLKNIAAILSEAGTDFDHVVKTTCFLKDMNDFVAFNEVYKTAFSADFPAR
                     SAVEVARLPRDVKVEIEVIAVID"
     gene            complement(4140..5459)
                     /locus_tag="AC1PIW_001639"
     CDS             complement(4140..5459)
                     /locus_tag="AC1PIW_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIP6606546.1"
                     /translation="MKQESLGKEIIRLALPATVENIFQTLVGFVDTLLIAQLGLVAVT
                     AVGLANTILNVYLAVYMALGVGATALIARSIGAGDRESLTFHVRQALVLSVGVGLLLG
                     LLSLVFGRQMLVLMGADAESLAGVQVFFYWVGGLTIFQALMTILGTILRASGDTVFPM
                     KMSLLTNGFNVVLDYFLIFGIGSWSGLGIVGTALGTVLARLLGTVLLYRKVQQTDLAV
                     DLKQLFHLGSPKEMVVLTLPAATERLVMRLGQVVYFSLIVGLGTIVYASHMIAGNIES
                     FTYMPAYGLATAAAVLIGQALGKGDILTVRRVAFLSSAYGVAIMSLLGTILFFGAPSF
                     ALLFTKDLEAVRQVVTALRIDAFNQPGLAVSLIMAGALQGLGDTKSPLYSTMIGMWGL
                     RVSGVIVLGQMFGLGIAGVWLSILIDLLLRAIFLTWRFIVQTRKLAE"
BASE COUNT         1669 a         1353 c         1002 g         1436 t
ORIGIN      
        1 ctataagcta ttttaccatc ctgcttcagt tgcttcaaaa catggctaac cgtttcccgt
       61 gtcgtagctg ctaaacgcga aatctcttga atatgaattt caaatggcaa ggtagcaaac
      121 tgctctcgct ggcacatatc ccaataaaga agagccaagg cttgaacgac tcgatctgat
      181 gccttggaac gcatcgcatt tcgcaagcgc aactcttgaa aacgaagcac tttggataat
      241 ttttgacaga tataagccat ttgactcgga tttatcttcg acaaggtttc aaacaagacc
      301 atcggtacaa taaaatactc taggtccgtt atggcaatgg cggtataatg ataaggtttg
      361 tcctgaaaca tatctccaaa aggaaaggca ccaccctgcc gaatatagtc taaatacgta
      421 aaactatccg tctcatcata ttgctcaatc cgagcatagc ccttatacaa tacaaataga
      481 taatctctag gatcctctgc gtaaaaaaag atttgtccct taggaatctt tcgaaaacga
      541 atatgcttag ctaattgatc aaagctatca cgtgtcaaac gttcaaaagt tggatgtgcc
      601 cttagataat catattgttc gttgcttatc atcaaaactc ctttctagct aaagtatcct
      661 tctatctatt ttaacatata ttaacgttct atgctaatca ttactgaaaa attcataaaa
      721 caggatcatt cctgtgaaga ggaatactat cacaactaat acaaaaaact ggtcctctcg
      781 aaccagtctt agtatcgtcg aatgaattaa ctttcagaca aggaactgag gtgtaagcaa
      841 tactagagta cggcaagccg aaagtaacga tgtatcaaag ttaattcaaa tgtccataac
      901 attctatgta tacctatcta gtcagtcaat tcatccgcaa tcttattgat tttccgcaaa
      961 cgatggttga caccactttt agtgatcggt accgttagac tatccgccaa ttgctgaatg
     1021 gaatagtctg gatgctgaat tcgcaactgg gcaatctcct gcaagtcacc tgacaactga
     1081 tctagaccga tagtatccat aattttgata atattattga ttgttttcat actagcattg
     1141 accgtctttg caatattagc agcttccgca ttggtagctc gatttaggtc attgcgagcc
     1201 tcacgaagca atttaacatt ctcaaattct gtcttcgtct cttctgctcc aatcaccaaa
     1261 agaaaatcca taatgtcctc tgcacgttgc agataggtaa tggttccctt acttcgctca
     1321 atgactttgg catccaaaag aaatttctgc atcagattgg ccaggtcatt ggcatggtca
     1381 ctatagacag aagctatttc aagctggtat tttccttttt caggatcctt aacagagcct
     1441 gccgctaaaa atgctccacg aagataggct tgactccacg aatcattttc caaaaccaag
     1501 ggagaaatcc ccgtctctaa tccaaagaag ctatcagcca aatgcaaatc gttcaatatc
     1561 tcattcacgc cgtcttctat caaaactgca tacacacgat tctttttgag attaggtttt
     1621 tgatgatggc gaatctcagc tttaatctgg tagaaatgga gtaacagttc ataaatgtga
     1681 cgggcaatct ttgcattttc cgtgctgata gaaagagtca aacctgatga agctaagccc
     1741 aaactacctg ataatttgat aatagcagat agctcgctct tattttggct agactgaagc
     1801 agcaattctt cctttacttg tactgtaaaa ctcatggccg cacctgcaaa atcttgagca
     1861 actcttctac taccaagtca ccatcatgga aagctccgcc attttccaaa cgaagaaaat
     1921 tcgaagaaat cacacgatta gcttgttttt gcaaacctgc aaaatcgtgt tttacctgta
     1981 ccaagtattc atcaaactgg ttgctattca tatactcttg tggaactggc tcaatattga
     2041 ccaaaactgt atcaatgaat tgttcagcta agtgtgcatt gagaaccgcc acgtggtccg
     2101 catctgagaa aaactcagtc tctccccgtt gggtcataat gttgcacaca taggtcactt
     2161 cagccttggt ttcttttagg gctttgccaa tgtcagaaat catcagattc ggcaagattg
     2221 aggtaaaaag agaacccggt ccaagtacaa tcatgtcact ttccataatc gtttcaacta
     2281 cctggcgact ggctttcggt tcatcatcat tataagaatt tgtcacatag acatgatcaa
     2341 tcatcccatt atgcttggaa atcttactct cacctgctac ctcggtccca tctgtaaaaa
     2401 tagcatgaag ggtcaaggcc tgttcgctag atggataaat ccgccctgtc gtatggaaaa
     2461 agcgcgtcaa caacctcatc gcattgtagg ttgaaccctg catttctgaa ataccagcaa
     2521 tgatgagatt ccctagtgga tgaccagcca aggggccatc tgaatccgca aaacgatact
     2581 ggaaaatttg ttcataaagt ttgggcatat ccgacatagc caataggacg ttacgcaaat
     2641 cacctggagg ggtcacctgc aaagcctgac ggatttcccc cgaagatcct ccgtcatcag
     2701 ctaccgtcac gatagcggta atctctacat ccttatcccg caaactcttc aaaatgactg
     2761 gaataccagt cccgcccccg ataactgtaa tctttggttt tctcatgagc ggttgaccgt
     2821 ctccttccgt ctgtccttat cacgatgact acgattaaca ttccaatttt tctctaaatc
     2881 gtctgccaaa cgtttagcaa aggctacgct tctatgctga ccacccgtac atccaacggc
     2941 aatcgttaga actgattttc cttctttctg ataaccagga agaatcggtt ctatcaagcc
     3001 gagcaaatga ctataaaact cttccgattc ttgatgctcc atgacatagt caaagacagg
     3061 agcatccaga ccagtcagat tacgcaactc caacttgtag tagggatttg gtaagaaccg
     3121 aacgtcaaaa accaagtctg catccagagg tagtccatat ttgaaaccaa aggacatcac
     3181 ttctatgcga aacgacggct gattatcttg actagcaaac tgctctgaga ttaccttacg
     3241 gagattgcga ggtgtcagct ctgtcgtatc aatgacattc tgactcatat ttttcaaggg
     3301 agccaaaagt tcacgttcta gctgaatccc gtccaaaaca cgaccatcag ccgccaaagg
     3361 atgggaacga cgagtttctt tatagcgagc aaccaattca ctatccgtcg catccaagaa
     3421 taggacttta aaatctaaat cctctgcccc ctcaatctca tccaacacct ctcgaatctc
     3481 cgagaagaag gatcgactcc gcatatctac aacaagagca atcttattgt tatcctggct
     3541 atgacgaatc aactctaaaa actttggcaa gagagtcggt ggcatattgt caatggtaaa
     3601 ataacccaag tcctcaaaag actgaatggc tactgtctta ccagctcccg acatccctgt
     3661 cacaatcact aaatggagtt tgtccgacat gacatcccct ccttttctct tctaatcaat
     3721 aactgcaata acttcaatct caaccttaac atcacgtggc aagcgtgcaa cttccaccgc
     3781 tgaacgagct ggaaaatctg ctgagaaagc tgttttataa acttcattaa aggctacaaa
     3841 atcattcata tcttttagga aacaagtcgt cttcaccaca tggtcaaagt ctgttcctgc
     3901 ttctgataaa attgctgcga tatttttcaa gacttgctca gtctgctcct gaatggtttc
     3961 accaaccact tcaccagttt caggtgacaa aggaacttga ccagaggcaa ataggaaatt
     4021 tccaacaacc ttcccttgaa cgtatgggcc aattgctgca ggtgccttat ctgtgtgaat
     4081 ggttttcatc tgattatctc ctttttatca ttctactgat attataccaa aaaactagcc
     4141 tattcagcca gttttcgtgt ttgcacaata aacctccaag tcagaaaaat cgctcgcaag
     4201 agcaggtcaa tcaaaatcga cagccagacg ccggcaattc ctaaaccgaa catctgacct
     4261 aagacgataa cacctgagac gcgcagcccc cacattccta tcattgtgga gtagagtggc
     4321 gacttggtat cccccaagcc ctgcagagcg ccagccatga tcaaagaaac cgcaaggcct
     4381 ggctggttga aagcatcgat ccgtaaggct gtgacgactt gacggacggc ttctaggtct
     4441 ttggtaaata gcagagcaaa acttggggct ccgaaaaata agatggtacc gagcagggac
     4501 atgatggcta ctccataggc agagctgaga aatgcgaccc gtctaactgt gaggatgtcc
     4561 ccctttccca aggcttgacc gatcaaaacc gccgccgctg tcgcaagacc ataggcaggc
     4621 atataggtaa aactctcgat attacctgca atcatatgag aagcatagac aatcgttccc
     4681 agccccacaa tcaaactgaa atagacaacc tgtcccaacc gcataaccaa gcgttcggtt
     4741 gctgcaggta gggtcaaaac caccatttcc ttaggacttc ccaagtggaa caattgtttg
     4801 agatcaactg ccaagtcagt ctgctgtacc tttcggtaaa gcaagacagt accaagcaag
     4861 cgagctaaga cagttcccaa ggcggtccct acaataccca aacctgacca agagccaata
     4921 ccaaaaatca agaaatagtc caagaccaca ttaaaaccat tggtcagtaa gctcattttc
     4981 ataggaaata ctgtatcccc agatgcccgc aggattgttc ccaaaatagt catcaaggcc
     5041 tgaaaaatag tcaaaccacc aacccagtag aaaaagacct ggacgccagc caaactctcc
     5101 gcatctgctc ccattaagac caacatctgc cgcccgaaaa caagggatag taaccccaat
     5161 agcagaccta cacctactga aagcactaag gcttgacgga catgaaaggt aagagattcc
     5221 ctatctcctg cgccaataga ccgagcaatc aaagctgttg ccccaactcc caaagccata
     5281 tagaccgcca aatagacatt caaaatcgta ttggcaagac caactgctgt caccgctacc
     5341 agccccagct gtgcaatcaa gagggtatcc acaaaaccaa ctaaggtctg aaagatattt
     5401 tccaccgtag caggcaaggc caaacgaata atttcttttc ctaaactttc ttgcttcatt
//
LOCUS       DBNFCK010000113         5444 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.113, whole genome shotgun sequence.
ACCESSION   DBNFCK010000113 DBNFCK010000000
VERSION     DBNFCK010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5444
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.113"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..394
                     /gene="atpF"
                     /locus_tag="AC1PIW_001640"
     CDS             <1..394
                     /gene="atpF"
                     /locus_tag="AC1PIW_001640"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIP6606547.1"
                     /translation="TGIFEERANKIANDIDAAEEKLTAAANLVQQREDELVQGRIESQ
                     KIIQDAVERAKLEKKRILEQADVEIQGLKQKAQLEIEAEKREAQENLRVQVAELAVDL
                     ASKIILEDLDQQAHSNLIDRYLDKLGDK"
     gene            395..928
                     /locus_tag="AC1PIW_001641"
     CDS             395..928
                     /locus_tag="AC1PIW_001641"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HIP6606548.1"
                     /translation="MNARENAIVQKYALSFVEKVSDHADIWDMYDQISDLISIIHDSK
                     LNRILLSATVSREEKADFVRTVRQSSFWQINDLIEDVIRDGHADLLLETLERVQLQIS
                     KFKNEFEARVVSVYPLTEAQKERLRHLVEQRFSLRVRNITEELDQSLLGGFIVTVNHK
                     VIDASVRTQLKDIRKKL"
     gene            932..2452
                     /gene="atpA"
                     /locus_tag="AC1PIW_001642"
     CDS             932..2452
                     /gene="atpA"
                     /locus_tag="AC1PIW_001642"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIP6606549.1"
                     /translation="MESGVLLINAQEISALLKQQIEGFQPDFDYTETGVVTYIGDGIA
                     RAQGLDNAMSGELLVFENGTIGMAQNLETNDVGIIILGQFTDIREGSVVRRTGKIMEV
                     PVGSALIGRVINPLGQPVDGLGEIRTSKTRPIEYPAPGVMQRKSVNEPLQTGLKAIDA
                     LVPIGRGQRELIIGDRQTGKTSVAIDAILNQKGQDMICIYVAIGQKESTVRTQVETLR
                     QYGALDYTIVVTASASQPSPLLFLAPYAGVAMAEEFMYEGKHVLIVYDDLSKQAVAYR
                     ELSLLLRRPPGREAYPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAY
                     IATNVISITDGQIFLKDDLFNSGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLA
                     SYRELEAFTQFGSDLDAATQAKLNRGRRTVEVLKQPLHKPLPVEKQVLILYALTNGFL
                     DSVPIDDILAFEEELYAYFDLHYDGLLDVIRTTKDLPDTDELNAAIQAFKDQSVFK"
     gene            2464..3342
                     /locus_tag="AC1PIW_001643"
     CDS             2464..3342
                     /locus_tag="AC1PIW_001643"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HIP6606550.1"
                     /translation="MAGSLNEIKSKIASTKKTSQITGAMQMVSASKLAKSEQLAQSFQ
                     IYASKVRQITTDLLRGELMASDTSNPMLIRRPVQKSGYIVITSDSGLKGSYNSSILKA
                     VMGMIEQDHDSKDEYEIIAIGSMGADFFRARGINPVFELRGLADNPSFEEVQKIISKS
                     VEMYKNELFDELYVCYNHHVNSLTSQVRVQQMLPVEDLDHNEADGYTATFELEPNREV
                     ILEQLLTQYAESTIYGAILDAKTAENAAGMTAMQTATDNAKNVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            3362..4768
                     /gene="atpD"
                     /locus_tag="AC1PIW_001644"
     CDS             3362..4768
                     /gene="atpD"
                     /locus_tag="AC1PIW_001644"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIP6606551.1"
                     /translation="MSSGKITQVVGPVVDVAFAAEDKLPEINNALVVYKNDDSKQKVV
                     LEVALELGDGVVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EESFPADFEREPIHKKAPAFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLTQ
                     LGIYPAVDPLASSSRALAPQIVGEEHYAVAMEVKRVLQRYQELQDIIAILGMDELSDE
                     EKTLVGRARRIQFFLSQNFNVAEQFTGMPGSYVPVAETVKGFKEILDGKHDHLPEDAF
                     RNVGSIEDVVAKAAKMKF"
     gene            4778..5194
                     /locus_tag="AC1PIW_001645"
     CDS             4778..5194
                     /locus_tag="AC1PIW_001645"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HIP6606552.1"
                     /translation="MGQMTVQIVTPDGIKYDHHAAFVLVKTVDGEMGVYSGHEELIAV
                     LEIGEMKVRRVDDENHVDWIAVNGGIIEVNKDIITVISDSAERERDIDISRAERAKLR
                     AERELEEAQTARNIDMEMRATVALQRAINRIRVGKH"
     gene            5254..>5444
                     /locus_tag="AC1PIW_001646"
     CDS             5254..>5444
                     /locus_tag="AC1PIW_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HIP6606553.1"
                     /translation="MVISILQFFSHILFIYLAHHLLVTTVDWSRWLKVTGDNQRKINL
                     LILFLAIALGYLVSTFFLE"
BASE COUNT         1556 a         1021 c         1287 g         1580 t
ORIGIN      
        1 tacaggtatt tttgaagaac gtgccaataa gattgcgaac gatattgatg ctgcagaaga
       61 aaaattaaca gcagcagcaa accttgttca acaacgtgaa gatgagttgg tacagggacg
      121 tattgaaagt caaaagatta ttcaagacgc ggttgaacgc gcaaaactag agaaaaaacg
      181 tattcttgag caagcagatg ttgaaattca aggattgaag caaaaagctc aattggaaat
      241 tgaagctgaa aaacgtgaag cacaagaaaa cttgcgtgtt caagttgctg aattggcagt
      301 tgatcttgca agcaaaatta ttttggaaga tttggaccaa caagctcata gtaacttgat
      361 tgaccgttat ttggacaaac taggagacaa gtagatgaac gctagagaaa atgccatcgt
      421 gcaaaaatat gctctatcat ttgttgaaaa agtcagtgat catgcagata tttgggatat
      481 gtatgaccag atttctgact tgatttccat cattcatgat agtaagctga atcgaatctt
      541 gctgtcagcg acagtatcga gagaagaaaa ggctgacttt gtaaggacgg tacgtcaatc
      601 tagtttctgg caaatcaatg acttgattga agatgttatt cgtgatggtc atgcagactt
      661 gcttcttgaa acccttgagc gtgtccaatt acaaatcagt aaatttaaaa atgaatttga
      721 agcccgtgtg gtttcagtct atccgttgac agaagctcaa aaagagcgtc tccgtcactt
      781 ggtagaacaa cgattttcct tgcgtgtacg caatatcacg gaagaacttg atcaaagtct
      841 cctcggtggt tttattgtaa cggttaacca taaggtaatc gatgcaagcg ttcggacaca
      901 gttgaaggac attagaaaaa aactttaaaa aatagaaagt ggtgttcttt tgattaatgc
      961 acaagaaatt agcgctttac taaaacaaca aattgaaggc tttcagcctg actttgacta
     1021 cacagagact ggtgtcgtaa cctatatcgg tgacggtatc gcccgtgcgc aaggtcttga
     1081 caatgccatg agtggagagc ttttggtatt tgaaaatggc accattggta tggcacaaaa
     1141 cttagaaacc aacgatgttg gtattatcat tctcggacaa tttaccgata ttcgtgaagg
     1201 gtcagttgtt cgtcgtactg gtaaaattat ggaagtacca gttggttcgg ctttgattgg
     1261 tcgtgtcatc aacccacttg gtcaacctgt ggatggtctt ggtgagattc gtactagtaa
     1321 gactcgtccg attgaatatc cagcaccagg tgttatgcaa cgtaagtcgg ttaatgaacc
     1381 gcttcaaact ggtctcaagg cgattgacgc tttggttccg attggtcgtg gtcagcgtga
     1441 attgattatt ggtgaccgtc agacagggaa gacatctgta gctattgatg ctattctcaa
     1501 ccaaaaaggc caagacatga tttgtatcta tgtggctatc ggtcagaaag agtcaactgt
     1561 tcgtactcag gttgaaaccc ttcgtcaata tggtgctttg gattacacaa tcgtagtaac
     1621 tgcatctgct tcacaaccgt ctccattgct tttcttggct ccttatgctg gtgttgcgat
     1681 ggcagaagag ttcatgtatg aaggcaaaca cgtcttgatt gtttacgatg atttgtcgaa
     1741 acaagcggta gcttaccgtg aattatctct cttgcttcgt cgtcctccgg gtcgtgaagc
     1801 ctatccaggg gatgtattct acttgcacag tcgtttgctt gagcgttcag caaaggtttc
     1861 agatgaattg ggcggtggct ccattacagc tctaccattt atcgaaacac aggcgggaga
     1921 tatttcagct tacatcgcga ccaacgtgat ttctatcacg gacggacaaa tcttcttgaa
     1981 ggacgattta ttcaactctg gtatccgtcc agccattgat gcgggttctt ctgtatcacg
     2041 tgtaggtggc tctgcacaga ttaaggccat gaaaaaggtt gcaggtaccc ttcgtatcga
     2101 cttagcttct tatcgtgaat tagaagcctt tactcaattt ggttcggatt tggatgcagc
     2161 aactcaggct aaactcaacc gtggtcgtcg gactgtggaa gtgttgaaac aaccattgca
     2221 taagccgctt ccagttgaaa aacaggtttt gattttgtat gctttgacaa atggcttctt
     2281 ggattcagtg ccaatcgatg atattttggc atttgaagaa gaactgtacg catattttga
     2341 tttgcactac gatggtcttt tggatgttat tcgtacaaca aaagatcttc ctgatacaga
     2401 tgaattgaat gctgctatcc aagctttcaa agaccagtct gtctttaagt aaaggagtga
     2461 ataatggcag gttctctcaa tgaaatcaaa tcaaaaattg catcaactaa gaaaaccagt
     2521 caaatcaccg gtgccatgca gatggtttcg gcttctaaat tagccaaatc agagcaatta
     2581 gcgcaatcct ttcaaatcta cgctagcaag gttcgtcaga ttacaacgga cctcttgcgt
     2641 ggagagttga tggcttcaga tacaagcaat ccaatgctga ttcgtcgtcc agttcaaaaa
     2701 tctggttata ttgtcattac ctctgacagt ggactaaaag gttcttataa ttccagtatt
     2761 ttgaaggctg tgatggggat gattgaacag gatcatgact caaaagacga gtatgagatt
     2821 attgccattg gtagtatggg agctgatttc ttccgtgccc gtggtatcaa tcctgtcttt
     2881 gaattgcgag gtttagcaga caacccaagt tttgaagagg ttcaaaaaat catttctaag
     2941 tctgttgaaa tgtacaagaa tgaactcttt gatgagttgt atgtttgtta taaccaccat
     3001 gtcaatagtt tgaccagtca ggtgcgtgta caacagatgc ttcctgtaga agatttggac
     3061 cacaatgaag cagatggtta tacagctacc tttgagttgg aaccaaaccg tgaggttatt
     3121 ttggaacaac tcttgactca atatgcagaa tcaactattt atggagcgat tttggatgcc
     3181 aaaactgctg aaaatgcagc aggtatgaca gccatgcaga cagcaacgga taatgctaag
     3241 aatgtcatta acgatttgac aatccaatac aatagggctc gtcaggcagc gattactcag
     3301 gaaattactg agattgtagc tggtgcctca gcccttgagt aatagaaata gaggaaatag
     3361 aatgagttca ggcaaaatta ctcaggttgt cggaccagtt gtagacgttg cgtttgcagc
     3421 agaagataaa cttcctgaga ttaacaacgc actcgttgta tataaaaatg atgattccaa
     3481 acaaaaagtc gtgcttgaag tggctttgga acttggtgat ggcgttgtac ggaccattgc
     3541 catggaatca acggatggat tgacacgtgg gatggaagtt ctcgatacag gtcgtcccat
     3601 ctctgttcca gtcggtaaag aaacgctggg tcgtgtcttc aatgtgttgg gagataccat
     3661 tgaccttgaa gagtcttttc cggcagattt tgaacgtgag cctatccata agaaagcgcc
     3721 ggcttttgac gaattatcta cttcaagcga aattttggaa acagggatta aggttatcga
     3781 cctcctagca ccttatctaa aaggtggtaa ggttggtctc ttcggtggtg ctggtgttgg
     3841 taaaaccgtt cttatccaag aattgattca caatattgcc caagaacacg gtggtatctc
     3901 tgtatttacc ggagttggcg agcgtacccg tgaagggaac gatctttact gggaaatgaa
     3961 agaatcaggt gttattgaaa aaacggccat ggtatttggt cagatgaatg agccaccagg
     4021 agcccgtatg cgtgttgctc ttactggttt gactattgcg gaatacttcc gtgatgtgga
     4081 agggcaggat gttcttctgt tcatcgataa tatcttccgt ttcacacagg ctggttcaga
     4141 agtgtctgcc ctcttgggtc gtatgccatc agccgttggt tatcagccaa cacttgcgac
     4201 ggagatggga caattgcagg agcgtattac ctcaaccaag aagggttctg ttacatctat
     4261 tcaggctatt tacgtacctg cggatgacta tacagaccca gctccagcga cagccttcgc
     4321 tcacttggat tcaacaacta acttggagcg taagttgact cagcttggta tctaccctgc
     4381 ggtggatcca ttggcttcgt catcacgtgc acttgctcca caaatcgttg gtgaagagca
     4441 ctatgcagta gctatggaag taaaacgtgt ccttcaacgt taccaagaat tgcaagatat
     4501 tattgccatt ctcggtatgg atgaattatc agatgaagag aagaccttgg ttggtcgcgc
     4561 tcgtcgtatc caattcttcc tctctcaaaa cttcaacgtt gcggagcaat ttacaggtat
     4621 gccaggttct tatgtgccag tagctgaaac ggtgaaaggc tttaaggaaa tcttggacgg
     4681 caaacacgac catctaccag aagatgcctt ccgaaatgtt ggttcaattg aggatgtggt
     4741 cgctaaagct gctaaaatga aattctagag gtggcttatg ggacaaatga ctgtacaaat
     4801 cgtaacacca gacggtatca aatatgacca tcatgcagca tttgttttgg tcaaaacggt
     4861 tgatggtgag atgggtgtct attctggaca tgaagaactg attgcagttt tggaaatcgg
     4921 tgaaatgaaa gtgcgtcgtg ttgatgatga aaaccatgta gactggattg cggtcaatgg
     4981 tggaatcatt gaagtcaaca aggatattat tacggtcatt tctgattccg ctgagcggga
     5041 acgggatatt gatattagtc gtgcggaacg tgccaaattg cgcgcggaac gtgaattgga
     5101 agaagcacag actgctcgta atattgatat ggaaatgcgt gcaacagtcg ccctccaacg
     5161 tgcgataaat cgtattcgtg ttgggaaaca ttaatctata tgggattgaa cttggttcag
     5221 ttccattttt taatttcatg gtataatgga aacatggtaa tttcaatttt acagtttttt
     5281 agtcatatat tgtttatcta tctggctcat catctcttgg ttacaacagt tgattggtca
     5341 cggtggttga aggtgactgg ggacaatcag cgtaaaatca atttgctgat tctctttctg
     5401 gcaatagcac tggggtactt agtctcgact ttctttttag aatt
//
LOCUS       DBNFCK010000114         5250 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.114, whole genome shotgun sequence.
ACCESSION   DBNFCK010000114 DBNFCK010000000
VERSION     DBNFCK010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.114"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(67..660)
                     /locus_tag="AC1PIW_001647"
     CDS             complement(67..660)
                     /locus_tag="AC1PIW_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIP6606554.1"
                     /translation="MTQIKWRDCLLICFGAILYALVLNMLIIPHEFGEGGITGVTLLL
                     FYTTGIETSLSSFVLNGILIVLAYRFLSKLTVFYTFLAVATMSLTMHYTTFLQIKWLP
                     LIPSALLAGLVTGLSMALVIYGNGSTAGSDIIALLCNKYFKWKISTVILIFDVFVVTP
                     LAFKIGLVKTFWTLVMVVIISKSLDWFLQALKKNNHK"
     gene            complement(738..1121)
                     /locus_tag="AC1PIW_001648"
     CDS             complement(738..1121)
                     /locus_tag="AC1PIW_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIP6606555.1"
                     /translation="MGLFSGLLGNASQMDNDKIEQELEHVLLDNEQVEMAFSLVRDLI
                     VFTEYRLILVDKQGMTGKKVSYKSIPYRSISRFTVETSGHFDLDAELKIWISSAVEPA
                     EILQFKSDKSVIAIQKALASAVLGK"
     gene            complement(1212..3788)
                     /locus_tag="AC1PIW_001649"
                     /pseudo
     CDS             complement(1212..3788)
                     /locus_tag="AC1PIW_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647159.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            4172..4924
                     /locus_tag="AC1PIW_001650"
     CDS             4172..4924
                     /locus_tag="AC1PIW_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606556.1"
                     /translation="MENTSTQTFEELVAEQKKKRNRVLRIIITLFTTIIVFFACLALF
                     KYNSGNISGTWRNSQAEQDIVETSIKELQNQLPGIDKTWIDATYTMTVVDKKAVFTAT
                     TSFDIPKIAKLTLDQAYKVMLEEAQKEASSLGISVEEYLTTALGADYEKQLKSFLPTQ
                     AEAEKEFHKYQDEYAKELGATYNQETGTISATFFEGDVDQLFHTVTITKINDAAIKQL
                     TTYDISVKDIISYSRKGNTTIINGNEEFTLEK"
BASE COUNT         1687 a         1034 c          844 g         1685 t
ORIGIN      
        1 aattcattcg actataagaa aagagaaacc tagttataca tgcacaacta ggtttctctt
       61 gtatttttat ttatgattat tctttttcaa tgcttgcaag aaccagtcca aacttttact
      121 gataatgaca accattacca atgtccaaaa tgtttttaca agcccaattt taaaagccaa
      181 aggagttacc acaaatacat caaaaatgag aataacggtg gaaatcttcc atttgaaata
      241 tttattacac aacaatgcaa taatatcact tcctgccgtg gacccgtttc cataaatgac
      301 tagagccatt gataaacctg tcaccaatcc cgccaagaga gcagagggaa tcaggggcaa
      361 ccattttatt tgcagaaaag tcgtataatg catggttaag gacatagtag caaccgctaa
      421 gaaagtatag aaaaccgtta acttactcaa aaagcgatag gctaatacaa tcaaaatccc
      481 attcaaaaca aaactggaca gacttgtttc aatccctgtc gtataaaaca aaagtagggt
      541 aacaccagta atcccacctt cgccaaattc atgtggaatg atgagcatat tcaaaacaag
      601 ggcatatagg attgccccaa aacatatcaa taagcagtct ctccatttta tctgcgtcat
      661 attctctcct cacaaacagt ccaccttagt atatcaaaaa aagcagaaag cggatagcca
      721 ctttctgtac ttttttccta ttttcctaag acagctgaag ccagagcttt ttgaatagca
      781 atgacactct tatcgctctt aaactgaaga atttcagcag gttcaacagc cgatgaaatc
      841 catattttca gctcagcatc taagtcaaaa tgaccagagg tttctaccgt aaaacgtgaa
      901 atcgagcggt aaggaatcga tttataagat acctttttcc cagtcattcc ttgcttatcg
      961 accaaaatca atcgatattc tgtaaaaaca atcagatcac gaaccagact aaatgccatc
     1021 tcaacctgtt cattatccag tagaacatgc tccaattcct gttcaatctt atcattatcc
     1081 atttgcgagg cattgcctag taaacctgaa aatagtccca taatatgact cctttggtat
     1141 ttttctatag tataacacaa aaagctacta gttgactatc ttaggcacta ggtctttacc
     1201 aaacatgaga attactcgct tacctttgct tccttatcaa cttctttagg ttgattgttc
     1261 tcaatactgc caccttcaac tgctggaaaa tgttctgcaa tcgcagatgg gatcttctga
     1321 tcatttagat ttaattcttc cttcggaatg tgattttcac tcacatcaat tgttttcaaa
     1381 gtcttattcg gctctgtaat ggcaagtgtt tcaatatcat tttttgaagc attcaggtct
     1441 tctaaggctt tgtaatcatt gactccttct aaattcttca attgattatc tgcaacattt
     1501 aatcgaacaa ccgattcttg tttaccctct atttccaaag tcttaatttg attcccctct
     1561 acatccagat ttactaattt ttcattttct tctatccctt gtaattcagt aatcttatta
     1621 ttcttaagag taagactagt taaatttgga ttgtttttta caaactgaag atcttttaca
     1681 ttactttcat tagctgtcag ttcttctaaa ttctctgttt ttagagttga tatatccaat
     1741 tgaggattct gtgatatata caaacgggtt aacttcttct tgttttgaag cgcagataaa
     1801 tctgtgatac gattattatc taaactaaca gctgtcaatt gtgacaaatc tgctaatggg
     1861 gataaatccg tcactttatt atgatctaaa tttaaagatt ccaaagcttt taatttagct
     1921 agaatagtga tatcttcaat gtcatttcct gcagcagaaa ctacttttaa gtttggaaat
     1981 tcttccaaaa agcccaaatc agaaacacca ttttgtgata agtcaattcc ctctaatgtt
     2041 ggaatttctt tcaaaaattg ataatcctta acgcctgtgc tagtcatcca taattgtttc
     2101 aattttttaa attgcaagat aggttttaca tcatcaattg gcgtaaaacc aatccctaac
     2161 acttcaacat ttggcatcaa gtccagaccg gctctcttta gcggatcttt tcgctgacca
     2221 atatttagat attttaccgt ttttaaccac tctctcatct tctcggaatc tgtttcatta
     2281 gatgggaaag gagtatcggc cgtagcatgc aaaatatctt caataatctc atcatcaaaa
     2341 cctaattgtt ttaaagtagc cattccaatt ttatcatgag catgtgggtc accgtgaggg
     2401 tcttcgattg gttttccaac ttcaactttc tcaatcaaga ttgtatgaga atggtcacca
     2461 tgagggtaaa cgaaagcagg tcctgcatct gtttctatca ccttgatttt tgcttttgaa
     2521 acatgcaggt tttcggctag gtagctcttt ttagcttctc gttcttcttc tacacttgag
     2581 ttaggtttcc caccttcttg tggtgacaac ggaatttcta cattaacttg agtggaagtt
     2641 gacgaatggt attctgctct agcagtctct aaatactgag taggaatata agactcccac
     2701 ggacttccta tcaaatgact gtacggaagt aaatgagtat gagtaccatg gccaatgagt
     2761 aaccctaacg ctgtttgacc ttagactccg ctaccatcaa aaaggaaacc gtcgcttgtt
     2821 ggataatcaa tacctgggaa agctttcttt gaattagact gagtggaagt atgccctttt
     2881 tctgtggtag acttgagtgg gacgaaatta tttcttgtgg tcggagtatg caacgaatga
     2941 ttatcaagaa cagtaatctg tccctttttt ccaatggtct ccccagtatt tccttgtccg
     3001 tattgcaaac ttgatttcca aatataatga taatgatctc catgacgtac cgtatagcca
     3061 ttcgcatctt cagccactac atccttcgga tcaaatacat aaccatcatc aaaagtttgt
     3121 gatagcttcg cttgctttgc gttggaatgc gagctggatt ggctattctc aatgtactct
     3181 ttgggtacca gataggacca tttgctttct tttaaatcct tatagaagat aaaatgtgaa
     3241 tgaccattat gttctaagac cagtccctca tccgtttttg aaagaatttg accttcattt
     3301 tcaaaaagaa atccatcatc cgtaggataa tcaacaccag ctaccccttt tttagcttga
     3361 gaagtaggtt tcttttgatt tttggtgagt ttcttagctt cagaaataga aattacatta
     3421 gcatctttat ccttgggata gtagaagtac tgctcattta cttttataat ataacctttc
     3481 tctagctgat actgaatatc ttctttattc aatttataat cagaatctgg aagggtatct
     3541 tttaaaaagt gtgccgcata cgggatcgga ccttcaaaaa agtatgactc ttctccatat
     3601 agacgcatat agccattaga tttaatttca gacactacaa tcgtattttc atctactttt
     3661 gagatattgc tactttgaac atcttgatga tttattgcag tattctctgt tttttggcaa
     3721 cccgccaaaa aaagatgaca agataggata atgcctgtca aggcaaataa ggttgttttc
     3781 tttttcattt ttgtcctcct aagctcatta accagttaac aattaactag tatacatttt
     3841 atttttgatt tttgcaagaa aaaacaactg acaatgattg ttcgtgaata aattttccat
     3901 tccacgacaa acgcatgaaa aatttgtttt ataaaccata ttttcacgac tgctgtcagt
     3961 ttttagtaga aaaaatgtaa tataatagcc ttggaaatgg gggtatcctt gttaaagtgc
     4021 aagttttaca aaaaccttaa aaaaatgcat acaacaagaa aatagttaca tacctttttc
     4081 ttcatgcgga ttgcatgttt ccatccctac gatcattgac aaagcaaaca aactatagta
     4141 tattaaaaat agggaaataa aaggagaata tatggaaaat acatctactc aaactttcga
     4201 agaactcgtt gcggaacaga aaaagaaacg taatcgcgtc cttagaatta ttatcacact
     4261 ctttaccact attatcgtat tttttgcctg tcttgcatta ttcaaataca acagtggcaa
     4321 cattagcggt acgtggcgta attcccaagc tgaacaagat attgtcgaaa ccagtatcaa
     4381 ggaattgcaa aatcaacttc caggtattga taaaacgtgg attgacgcaa cttatacgat
     4441 gacagtcgta gataagaaag ctgtcttcac cgctacaaca agctttgata ttcctaaaat
     4501 cgcaaaatta accttggatc aagcttacaa ggtaatgtta gaggaagcac aaaaagaagc
     4561 aagttctctg ggaatttccg tagaagaata cttaacaacc gctctcggag cagattatga
     4621 aaagcaattg aaatcatttc tacctacaca agctgaggcg gaaaaagaat ttcataagta
     4681 ccaagacgaa tatgctaaag aactaggtgc cacatacaat caagaaactg gtactatctc
     4741 tgctaccttt ttcgaaggag acgtcgatca gctcttccat actgtgacca ttacaaaaat
     4801 taatgacgca gcaatcaaac agctgactac atacgatatt tccgtaaaag atattatttc
     4861 ctattctcgc aagggaaata ccaccattat taacggaaat gaagaattta ctttagaaaa
     4921 ataataaaaa aagctgggaa agtcatccct gatggttccg acctcaccca cagaataatg
     4981 aaaataaata tgaagccctc actaggtgta taatcttagt gagggctttg tgcttgatga
     5041 gttaatgatg ttagtcatcg ccggaaaaac taacccactc tattgacgag ctctaaaaaa
     5101 tatgcaatag acatgagcat aagaactagc caataaaaac acagatagat tcaagctaat
     5161 tttgatgaaa tacagccgaa ttgtctgtgc ttttattttt gtctctacta tagataggac
     5221 aacattgtga cgatatttga aagaacttgt
//
LOCUS       DBNFCK010000115         5240 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.115, whole genome shotgun sequence.
ACCESSION   DBNFCK010000115 DBNFCK010000000
VERSION     DBNFCK010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5240
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.115"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(1..138)
                     /locus_tag="AC1PIW_001651"
                     /pseudo
     CDS             complement(1..138)
                     /locus_tag="AC1PIW_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148688.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(360..3458)
                     /locus_tag="AC1PIW_001652"
     CDS             complement(360..3458)
                     /locus_tag="AC1PIW_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HIP6606557.1"
                     /translation="MKLQFKQQQFQLDAVASVVDVFVGQGKHSGFDYHMDKGQGMGAE
                     LDMNFIGFRNAPIQLSQETVSQQVRAKQLSYGLKPSENLSIQKVDGRPSYNLTIEMET
                     GTGKTYTYIRTLMELNKQYGWLKFIIVVPSIAIREGVLKSFEIMADHFQMEYGKKPRY
                     FVYDSSRLGELDKFANSSDIQVMIINSQAFNATGADARRIHTEQESFRWRKPIDVIAA
                     TNPILIIDEPQSVEGKKTKERLEDFKPLFTLRYSATHKDKHDMIYRLDALDAYNKKLV
                     KKIAVKTVEQTATTGTQGYLYLQELVPQKSGSPKARIEFEVRTKSGDVKWVTKLVEEP
                     FALFEESGNLPAYQGGWTLSHFDAREGENSIQIGANRKLYVGEVIGETNEDDIRRIQI
                     RETIASHLQKEERLYKRGIKVLSLFFIDEVAKYKCYDEQNDAYNSTYAQMFEEEYEAQ
                     VKALLADLNLHGSNFWHYLNKHQEGNPVHAGYFSVDKIKKSDKVKFVDYKSATEKKNN
                     QSNDKDAYDLIMKDKERLLSFEEPIRFIFSHSALKEGWDNPNVFQICTLKNTGTETEK
                     RQKIGRGMRLAVDQKGVRQDEELLGADVHNINKLTVIANESYEDFAKNLQRELREILA
                     DRPSKVEVDFFVNKVLENEAGQKLTVTKEVAKEIERQLIKQDYIDSKGNLTATYFEQK
                     ETSQFTLSENLAGYEVAVKDILETIYDANRYQIERENDSEVVFAREINPDNYNRQEFK
                     RLWESIHHKSTYYVEFDDEELIEKSVKALNESLFVKKPSYMITEAEAREMQAKHGLDF
                     KVNKNGKRQSVLDKSSIQLRYDLLGEIASRTELKRKTVANILKQISPSKFDMFSYNPE
                     AFIQQVSRLINEQKASQVIEHIRYNILEETFDSSIFTDNPLSGKVSDANMLKSEKGVY
                     NYVKVDSGTERKFKEDLERFDDVMVYAKLPSKFKISTPLGDYNPDWAIAFRDGSVKHV
                     YFVAETKGSMSSLQLKGAELAKIECAKAHFKALKEKGLIADDRIYDVVDSYEKLLEIV
                     RG"
     gene            complement(3462..5176)
                     /locus_tag="AC1PIW_001653"
                     /pseudo
     CDS             complement(3462..5176)
                     /locus_tag="AC1PIW_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003615287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - obsolete DNA methylation
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
BASE COUNT         1393 a         1236 c         1013 g         1598 t
ORIGIN      
        1 tacacaaacg ccggaatatt tctcccaaac tacttcgata ttgattatag atgatttttc
       61 gtctatactt aggtgtgaag acaatgtggt agaacacagc cactttgtat gtgataaatt
      121 atgtgccttt tgtgccatac ttttctcctc tcgctttaca ataggcttga atacctttat
      181 tgtatcgcgt ttggagtttt ttatgtatga gcggctagtt tttgcccagc ctctttcagg
      241 ggacaacaaa agccttgact atggcaacaa tgcccaccag ataacaaatc gcaaatgtaa
      301 aaaacagaaa cgcacccata aatattctcc ttcagtggcg agaaggattg ctcagcaacc
      361 tatcctctca cgatttctaa taatttttca taggaatcaa ccacatcata aatccgatca
      421 tccgcaatca atcctttttc ttttagggct ttgaagtggg cctttgcaca ctcaatcttt
      481 gccaactccg cccctttcag ttgcaaacta gacatggaac ctttggtttc agctacgaag
      541 taaacatgct tgaccgaacc atctcggaag gcaatggccc agtctggatt gtaatcaccc
      601 agcggtgtgg aaatcttaaa cttgctaggc aatttggcat agaccatgac atcgtcgaat
      661 cgctccaagt cctctttgaa cttcctctcc gttccagaat caaccttcac ataattataa
      721 acacccttct cagacttgag catatttgca tctgacacct taccactcaa aggattgtcc
      781 gtaaaaatag aactatcaaa agtttcctct aggatattgt agcggatatg ctcaatcacc
      841 tgactcgcct tttgctcatt gatgagccta ctaacttgtt ggataaatgc ctcagggtta
      901 taggaaaaca tatcaaactt gcttggcgaa atctgcttca aaatattggc gactgttttc
      961 cgtttcaact ccgtgcgact ggcaatttca cccagcaaat catagcggag ttgaatagaa
     1021 gacttatcca gcacagactg acgcttgccg ttcttattta ccttgaaatc caaaccgtgt
     1081 ttcgcctgca tttcccttgc ttcagcctct gtaatcatgt aactaggctt tttaacaaac
     1141 aaggactcat tcaaggcctt aacagacttt tcaatcaact cctcatcatc aaattcgaca
     1201 tagtaggtgg acttgtgatg aatactttcc cacaaacgtt taaactcttg acgattgtag
     1261 ttatcagggt tgatttctct tgcaaaaacc acttccgaat cattttcacg ttcaatctgg
     1321 tagcgattgg catcgtaaat cgtttctaga atatctttta cagccacttc atagcctgcc
     1381 agattttcag atagagtaaa ctgacttgtt tctttttgtt caaaataagt agcggtcaga
     1441 ttccccttgc tgtcaatata atcttgctta atcaactggc gttcaatctc tttagccact
     1501 tccttggtga cagtcagttt ttgaccagct tcattttcca agaccttgtt gacaaaaaaa
     1561 tccacttcca ccttgcttgg tcgatcagcc aagatttccc tgagttctct ttgcaagttc
     1621 ttagcaaaat cttcatagct ttcattggcg atgacggtca acttgttaat attatgaaca
     1681 tcagctccta aaagctcctc atcctgacga acaccttttt ggtcgacggc tagacgcatc
     1741 ccacgaccaa tcttttgtcg tttttctgtt tctgtacctg tatttttcaa ggtacaaatc
     1801 tggaagacat taggattgtc ccagccttct ttgagggctg agtgagaaaa gataaagcga
     1861 ataggttcct caaaagacag cagacgttcc ttgtccttca ttatcaaatc ataggcatcc
     1921 ttgtcgttag actggttatt tttcttttcc gtagcagact tgtagtccac aaacttgacc
     1981 ttatccgatt tttttatctt atcgacagag aaataacctg catgaacagg attgccttct
     2041 tggtgcttgt tgagataatg ccaaaagtta cttccatgaa ggttgagatc agccaataaa
     2101 gccttgacct gggcttcata ctcttcttca aacatctggg cataggtgct attgtaggca
     2161 tcattttgct catcatagca cttatacttg gcaacttcat cgatgaagaa aagggatagc
     2221 accttgatac ctcgtttgta aaggcgttct tctttttgca ggtgactggc aatggtttcc
     2281 cgaatctgga tacgacggat gtcatcttcg ttggtttccc caatcacttc gcccacatag
     2341 agtttgcgat tggcaccgat ttgaatggaa ttttcacctt cacgcgcatc aaaatggctc
     2401 aaggtccaac cgccttgata ggctggtagg ttgcctgact cttcaaaaag agcaaaaggc
     2461 tcttcaacca atttggtcac ccatttgaca tcgcctgact tggtccgcac ttcaaactca
     2521 atccgagcct taggagaacc tgatttttgc ggaaccagtt cttgcagata aaggtagcct
     2581 tgtgttcctg tggttgcagt ctgctccacg gtcttaacgg caatcttctt gaccaacttt
     2641 ttattgtagg cgtccagggc atccagacga taaatcatat cgtgcttgtc cttatgagtg
     2701 gctgaatagc ggagagtaaa taggggcttg aagtcctcca agcgttcttt agtctttttc
     2761 ccctcaaccg actgaggctc atcgataatc aaaataggat tggtcgccgc aatgacatca
     2821 atcggctttc tccagcggaa actttcctgc tcggtgtgaa tacgacgggc atctgctcca
     2881 gtggcattaa aggcttgtga gttgataatc ataacctgaa tgtccgagga gttggcaaac
     2941 ttgtccagct cgcccaaacg gctagaatca tagacaaaat agcgtggctt cttgccatac
     3001 tccatctgga aatggtccgc catgatttca aaggacttga gcactccctc acgaatagca
     3061 atgctcggca ccacgatgat aaacttgagc caaccatact gcttattgag ctccatcaag
     3121 gtacggatat aggtgtaggt cttccctgtc cccgtctcca tctcaatagt caaattgtaa
     3181 gacggacgac catccacttt ttgaatagac aagttttcag atggtttaag gccatagctg
     3241 agctgcttag ctcgaacctg ctggctgaca gtttcctgcg aaagctgaat gggagcattg
     3301 cggaaaccga taaaattcat gtccagttcc gcacccattc cctgaccttt atccatgtgg
     3361 taatcaaaac cgctatgttt gccctgacca acaaaaacat ccacaacaga ggccaccgca
     3421 tctaactgaa attgctgctg cttaaattga agtttcatct cctacaccac cttcactttg
     3481 gtatttggtg cttcttcttt gaagatttgg cccaggttga ttttggctgc ggagttggca
     3541 aaggattcgt ccttaaaaac agcacgaagg ggctctgctt tggcgatttg acagaggacg
     3601 ctttctgaaa tctcatccgc aaagcaggca atcaaggcac catctgctac attataaatg
     3661 gctgtaccgt caatctgggt tttcgtaatc ggaagagata attccatgcc ccatgtcagc
     3721 ataatctgga agagcaggtc aaggtctgtc cgaccgtctt tgatgttgga aacactgtca
     3781 aataggcttt cttggctaaa gctgtttggt gtttggctaa catccttgaa gtttgaagtg
     3841 tctagcttat aagccctgaa gccaaaatct tgcttgccga ctgtttctgg gtgttcttct
     3901 tgaatctttt tagcagcccg acggatccgc tcacgagaaa tctggtcaat ggtttcatag
     3961 ccagcctcct tggcagcgga cttgtctgcc acctgctcgt ccagcgtaca gagtatgtac
     4021 ttacgattac cgccatcttc tgcattcaac tgcataacag catcagcagt ggttgcagaa
     4081 ccagcgaaga agtcgaggat caggccgtct ttttctgaat acaattgaac aatttgcttc
     4141 atcaacggaa ctggttttgg ataggtgaaa tatgccttac catcaaataa ttttttcaag
     4201 tcttggctag cagactgaga atgaccgaca tcagcgtact tccaaatcgt cattggagtt
     4261 actgtatttt ttacttctga taaaaatctt tttatcctag gcacattatc tccattagcc
     4321 ccgaaccaaa tacgattatc agcaataaat tcttcaaatc tttctttact cagcaaccaa
     4381 cttctcccgc ttggaggaaa aatagatctt ccgcttggag aaattatctc ataaatattt
     4441 gaaggaactg ctggtcctac tgataaatca ccacttgtcc aaggtcctct aacatcatta
     4501 tcaggatttt ggtatctact atttgagtct tcgcttctag gtattccatg attgacagca
     4561 aattctacat tttttgcata tactaaaata taatcatgac tttctgaaaa atgttttttc
     4621 aagtttacag gtgcaaatgc tctctcccag atgacatcag caacaaaatt ctcctcccca
     4681 aatatctcat cacagatagc tttgaggttg gcttgttcgt tatcgtcaat ggagattaaa
     4741 gataactcca ctatctttga ggaggttgcg ggcaaggact aggcgagagt acatcatatt
     4801 gagccagtcg gaatgatagc gggcggagga tttttcattc ttggtcaagc ccacccgttg
     4861 caaaccgtcc tcatcacgca gatccatttc atctgccagt tcttcctcgc tcatctggaa
     4921 tttatccgaa tagacaaagt cctttcctgt attgtaaggt gggtcaatgt aaatcatatc
     4981 aatcttgccc agataagact cctgcaagac cttgaggact tccaggttgt cccctgttat
     5041 aaagacattt tcagacttgt caaagtcaac actttcctca agatctgggc gaagggtctt
     5101 ggtggtcggt ttacctgcca ttttcatcac tactttctgt tagaacctca ggaaaaagct
     5161 gaccaatttt tgcaatgttg tctgataaaa catcttttga ccttaggtct agcttttcaa
     5221 cttgagtgta gtctccaagc
//
LOCUS       DBNFCK010000116         5230 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.116, whole genome shotgun sequence.
ACCESSION   DBNFCK010000116 DBNFCK010000000
VERSION     DBNFCK010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.116"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            50..1840
                     /gene="dnaG"
                     /locus_tag="AC1PIW_001654"
     CDS             50..1840
                     /gene="dnaG"
                     /locus_tag="AC1PIW_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIP6606558.1"
                     /translation="MLSKDKITEIKQALNIVDVIGETVALTKAGRNYVGLCPFHGEKT
                     PSFNVIEDKQFYHCFGCGKSGDVFKFVEEVRGVSFADAAAILAEKAGFQIDVTPSYHQ
                     EEKRVSPHQVLYDIHRDAAKFYHALLMTTKMGEEARAYLHQRGLTDDVIKTFQLGLAP
                     AEQNILYQKLSGQYDEESLLNSGLFNPSENNIIYDAFQARIIFPLADEYGRIVAFSGR
                     IWTEENRNNKQLAKYKNSRSTAIFNKSYELYHLDKAKAVIKKQRETYLMEGFLDVIAA
                     HRVGIDNAVASMGTALTREHVAHLAKFCKKIVLTYDGDKAGQAATMKALDELQDFQVE
                     IVSLPDNMDPDEFLQKNSEEALQQVLTKSRISDVEFLIQYLKPENPDNLQMQIEFVDK
                     IAPIIAKVSSITAQNSYIYKVADLLSDFDYNQVEQAVNAVRLNQRQERTQQVFQSQTG
                     QSYLPPVQTIARITSLIRTENHLLYRMVEHPYILNEFRLREDFYFATPELQAIYDMLK
                     KSGEITSFELSQLNDSAQQAWYRMQEERLPDEVSPHEIEELEIRRDKELLKKENQHLA
                     RKIREHSHVGNADIALDELQKLLDRRRQME"
     gene            1843..2961
                     /gene="rpoD"
                     /locus_tag="AC1PIW_001655"
     CDS             1843..2961
                     /gene="rpoD"
                     /locus_tag="AC1PIW_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HIP6606559.1"
                     /translation="MTNKKDKKTEVTTFDVQVAEFIRNHKKQGSATDDEINDQLVIPF
                     TLDADGIDDLLQRIQDAGISIVDKEGNPSARAMQVEEEPELSDEELLGSTSAKVNDPV
                     RMYLKEIGVVPLLTNEEEQELALAVEAGDPEAKQRLAEANLRLVVSIAKRYVGRGMQF
                     LDLIQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVET
                     INKLVREQRNLLQELGQDPTPEQIAERMDMTPEKVREILKIAQEPVSLETPIGEEDDS
                     HLGDFIEDEVIENPVDYTTRVVLREQLDEVLDTLTDREENVLRLRFGLDDGKMRTLED
                     VGKVFNVTRERIRQIEAKALRKLRHPSRSKPLRDFIED"
     gene            3130..3453
                     /locus_tag="AC1PIW_001656"
     CDS             3130..3453
                     /locus_tag="AC1PIW_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HIP6606560.1"
                     /translation="MTYTEDQIKDIQERIFHALEDVIDPELGIDIINLGLIYEIRFIE
                     GKAEIDMTLTTMGCPLADLITDQIHDVLKDVPEVTEVDVRLVWSPAWTVQKMSRYARI
                     ALGIK"
     gene            3634..3791
                     /locus_tag="AC1PIW_001657"
                     /pseudo
     CDS             3634..3791
                     /locus_tag="AC1PIW_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775157.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3878..5008)
                     /locus_tag="AC1PIW_001658"
                     /pseudo
     CDS             complement(3878..5008)
                     /locus_tag="AC1PIW_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564846.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            5104..5230
                     /locus_tag="AC1PIW_001659"
                     /pseudo
     CDS             5104..5230
                     /locus_tag="AC1PIW_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148688.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1597 a         1019 c         1096 g         1518 t
ORIGIN      
        1 gggagagtgt tcacttttta aattagctag tatgatatga ggagggaatg tgctttcaaa
       61 ggataaaatt actgaaataa aacaagctct caatatcgtt gatgttattg gtgaaacggt
      121 tgctttgact aaggccggac gtaattatgt cggtctttgc ccttttcatg gggagaaaac
      181 cccatctttc aatgtgattg aagataagca gttctaccat tgttttggat gtggtaagtc
      241 cggagatgta ttcaagtttg tcgaagaagt tcgtggtgtt tcttttgcag atgcggcagc
      301 tatcttagct gagaaggcag ggtttcaaat agatgtaact ccttcctatc atcaagagga
      361 gaaaagggtt agtccgcatc aggtactgta tgatattcat cgggatgctg caaagtttta
      421 tcatgccctt ttgatgacaa ctaaaatggg tgaggaagct agagcttatc tacaccaacg
      481 tggcttgaca gatgatgtca taaaaacatt ccagctaggc ttagctcctg ccgaacagaa
      541 tattctttac caaaaactat caggtcaata tgacgaagaa agtctattga attcgggttt
      601 gttcaatcca agtgagaata atattattta tgacgccttt caagcgagga ttattttccc
      661 cttggcggat gaatatggtc gaattgtagc tttttcaggt cggatttgga ccgaagaaaa
      721 tcgaaacaat aaacaattag ccaagtataa aaactctcgt agtactgcga ttttcaataa
      781 aagctatgaa ttgtatcatt tggacaaggc taaagcggtt attaagaagc aacgtgaaac
      841 ctatctcatg gaaggttttc tagatgttat tgctgctcat cgtgtaggga ttgataatgc
      901 agtggcttcc atgggaacag ctttgacaag agagcatgtt gcacacctag ctaagttttg
      961 taagaaaatt gttctgactt atgatgggga taaggctgga caggctgcta ccatgaaagc
     1021 gttggacgaa ctacaagatt ttcaggttga aatcgtgagc ttgccagata atatggaccc
     1081 agatgaattt ttgcagaaaa attcggaaga agccttgcaa caggttttaa ccaagtcacg
     1141 aatcagcgat gtagaatttt tgattcaata cttgaaacct gaaaatcctg acaatctaca
     1201 aatgcagatt gagtttgtag ataagattgc accaattatt gccaaagtat catccattac
     1261 agctcaaaat tcttatattt ataaggtggc ggatttactt tctgactttg attataatca
     1321 ggtggaacag gcagtaaatg ctgttcgtct gaatcagcgc caggagagaa cacaacaggt
     1381 atttcaatca cagactggtc agtcttattt gccaccagtc caaacgattg ctcgtataac
     1441 gagtcttatt cgaacagaaa accatttact ttatcggatg gttgaacatc cttatattct
     1501 caatgagttc cgtttacgag aagattttta ttttgcgacg ccagaattgc aagctattta
     1561 tgatatgtta aaaaaatctg gggaaattac cagttttgaa ctatcacagt tgaacgattc
     1621 cgctcaacag gcttggtatc gtatgcagga agaacgttta cctgatgaag tgtctccaca
     1681 cgaaatcgaa gaattagaaa ttcggcgaga caaggaattg ttaaagaagg agaaccagca
     1741 cctagcacgg aaaatccgtg aacattccca tgtgggaaat gcagatattg ccctcgatga
     1801 gttgcaaaaa ctcctcgata gaagaagaca aatggagtaa atatgacaaa taaaaaagat
     1861 aaaaaaacag aagtaactac ttttgatgta caagttgccg aatttatccg taaccataaa
     1921 aaacaaggtt cagctacaga cgatgaaatc aatgaccaac tggttattcc atttacgctt
     1981 gatgctgacg gaatcgatga tttgctccaa cgtattcagg acgcagggat ttcgattgtt
     2041 gataaagaag ggaacccttc tgctcgtgct atgcaggtag aggaagaacc agaattatct
     2101 gatgaagaat tgctcggaag cacttctgca aaagtgaacg acccagtccg catgtatttg
     2161 aaagaaattg gtgttgtacc tcttttgacc aacgaggaag agcaagaatt ggcattggcg
     2221 gttgaggcag gtgacccaga agcgaaacaa cgtttggctg aagccaactt gcgtttggtt
     2281 gtatccattg ctaaacgcta tgtcggccgt ggtatgcagt tccttgatct tatccaagaa
     2341 ggaaacatgg gcttgatgaa ggctgttgat aagttcgact actctaaagg tttcaagttt
     2401 tcaacctatg caacctggtg gattcgtcag gctatcactc gtgccattgc ggaccaagcg
     2461 cgtacaatcc gtatccctgt tcacatggta gaaaccatta acaaattggt ccgtgaacaa
     2521 cgcaatctct tgcaggaatt gggccaagat ccaactccag agcaaatcgc tgagcgtatg
     2581 gatatgacgc ctgagaaagt tcgtgaaatc ctcaagattg cccaagagcc tgtttcactc
     2641 gagaccccaa ttggtgaaga agatgatagt catcttggag attttatcga agacgaagtc
     2701 attgaaaatc cagttgatta cacaactcgt gtcgttcttc gtgaacagtt agatgaggta
     2761 ttggatactc ttactgaccg tgaggaaaac gtattgcgtc ttcgttttgg tttagatgat
     2821 ggtaaaatgc gtacgcttga agatgttggt aaagtcttta acgtaacccg tgaacgcatc
     2881 cgccaaatcg aagccaaagc cctccgtaaa ctccgccacc catcccgcag caaaccacta
     2941 agagatttta ttgaggattg attttgtctg ggagacaaaa tcaaccgctc gctgaaaaca
     3001 ggaaacgagc gagattttaa ccgctcccct tgaaattaga aagggagcgt aacgtccagt
     3061 ggactttttg acacaaactt cgtttgtttc ttttctagta ttgaacttgt gaaaaagaaa
     3121 gagagaaaaa tgacgtacac agaagatcaa attaaagaca ttcaggagcg tatttttcat
     3181 gctcttgaag atgtgattga ccctgagcta ggtattgata ttatcaattt agggctaatt
     3241 tatgaaatcc gatttattga gggcaaggct gaaattgata tgaccttgac gaccatgggt
     3301 tgtccattag ctgatctaat taccgatcaa attcatgatg tattgaagga tgtccctgaa
     3361 gtaacagaag tagatgtgag actggtttgg tcaccagctt ggaccgttca aaaaatgagt
     3421 cgttatgctc gaattgcatt aggaattaaa taaaaaaaca aaatggtttc gaacgtttga
     3481 agccgttttt ttattatggc ttcgagccag tttttctcgt agagaaaaac aagaaagcca
     3541 ccagctatgc tggtggctgc caaggctttg agtttccatt tctttttcct gttataatct
     3601 aattgttcag gttagatcaa ggaggaaaaa gaaatggcta aaaccagtta cagtttatca
     3661 catactaaat ggatgtgctc tatcacatag ttttcacacc taagtaccgt agaaaatcca
     3721 tttactacaa aataaggcag gacttaattg atattttccg tcatctatgt cgatgcaaag
     3781 gcgtggacat cacgtcatac gcatgaaaaa tttcttctct ccttttgtcc ccggaaataa
     3841 ctgctttaaa cttagtatct acctttttac aagcacctta tccccgattt ccaaataatt
     3901 gtggcaaata atcctccaaa tggacagaaa tatagcgttg tttgcggcgg taactcaatt
     3961 tctccttagg gaaggtcatc atttgtaaga gatgaagaca agccttacga atagcattta
     4021 aattgaaagc agctcgttta tctaaggttt ggttcttatc ttctcggtaa accacatcta
     4081 acaatcaatg taggctctca accgaccaat gaccacgaac aacctgagaa aaagtttgca
     4141 catcgccctt gaaactaagg ataaagtaac ggacctcttc agtgatgaca ccatctttat
     4201 caatgatatt cttagtcata ccaatcccac gaagtttttt ccatttcgga tgtcgttggc
     4261 tcagccattt cacatcgtga gaaacccagt agtccctcac ctcaatctga ctgcgtgcct
     4321 tttcaatggt ctgataatga cagcctttct ctgtcaacgt agataacaat ttggcgtcac
     4381 taaaatagag gtcaatatcc tcatggagat tgccttgatt tcctttgact gccaagcaat
     4441 aatcaccctt accgctgata atcacatccg caatgtctgt ctaggtgccc atggcatcaa
     4501 tcgtgacaac actcttacga aggtctagct gacacaagag gcgaggaatg gctgtgattt
     4561 cattactttt atcctcaaca gctacctgcc caaggctaat ccgattacca ccatcataag
     4621 cagtgacaat atgagtagga gattggtgtt tccctcggtt cccccgaatc gtttttccgt
     4681 caacggcaat caattttgaa aaatcagttg tttccgaact agcttcgaag gacagtttca
     4741 gttcctttaa gaaatctgga ttgaccatac tcacaacacg ttccaaggtg tcatgagaaa
     4801 gacagccgac actcaagtca acgtattctc ccaaaacaga ctcgttcatt tctatgaaat
     4861 cttccatctc tttccacgtt tcaacccctg ccaattgaca agcgaaaact agaaacagaa
     4921 tagtggataa gggataacga attttccatg cttgacggct gttgtactcc tctttgtgct
     4981 cttctatgga taaaagaaaa tcaattattg tagttcacct ctattctagt gtactacttt
     5041 gattgatctt tggttatgat tttcgctttc tgaaaacgtt tttttcttca tgcgtttggt
     5101 gtggtggaaa tcattgaggg acacatgatg ccagaccatg ttcatatgct tgtcttgata
     5161 tccccaaaac tttcgatttc cgattttatg ggatatttga aaagtaaaag tgctctcatg
     5221 atatttgata
//
LOCUS       DBNFCK010000117         5206 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.117, whole genome shotgun sequence.
ACCESSION   DBNFCK010000117 DBNFCK010000000
VERSION     DBNFCK010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5206
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.117"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(12..1418)
                     /locus_tag="AC1PIW_001660"
     CDS             complement(12..1418)
                     /locus_tag="AC1PIW_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIP6606561.1"
                     /translation="MSKKSIVVSGLVYTIGTILVQGLAFITLPIYTRVISQEVYGQFS
                     LYNSWVGLVGLFIGLQLGGAFGPGWVHFREKFDDFVSTLMVSSIAFFLPIFGLSFLLS
                     QPLSLLFGLPDWVVPLIFLQSFMSVVQGFFTTYLVQRQQSMWTLLLSVLSAVINTALS
                     LFLIFSMENDFIARVMANSATTGVFACVSLLFFYKKIGLHFRKDYLRYGLSISIPLIF
                     HGLGHNVLNQFDRIMLGKMLTLSDVALYSFGYTLASILQIVFSSLNTVWCPWYFEKKR
                     GADKDLLSYVRYYLAIGLFVTFGFLTIYPELAMLLGGSEYRFSMGFIPMIIVGVFFVF
                     LYSFPANIQFYSGNTKFLPIGTFIAGVLNISVNFVLIPTIGIYGAALATTASYLLLLV
                     LHYFVAKKKYAYDEVAISTFVKVIALVVVYTGLMTVFVGSIWIRWSLGIAVLVVYAYI
                     FRKELTVALNTFREKRSK"
     gene            complement(1427..2398)
                     /locus_tag="AC1PIW_001661"
     CDS             complement(1427..2398)
                     /locus_tag="AC1PIW_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 52"
                     /protein_id="HIP6606562.1"
                     /translation="MRRIYICHTMYQILISLLKMDVERDSLMSVDIIGHFPDVREQLQ
                     QHVHLIEETERSFDLYSLIARSKTKERLSLLQSYDEVIIFQDHRQVGHFLNKHRIPYS
                     LLEDGYNFFKDKRVCDLESIQSSVWKRLFYQWYFKPTYLIGSSLYCQSIEVNDLSLVQ
                     FDEAYKPFVEVPRKQLFDQASPEKVQALLQIFGARAIVADEESSQKRLLLLTQPLSWD
                     YHVTEEELLEIYVAGLAPYREDYTIYIKPHPRDGVDYSFLGKAVVLLPQGIPFELFEM
                     AGSIRFDIGMTYSSSALDFLNCFEEKVYLKDTFPLLSKNDILREGIE"
     gene            complement(2835..2948)
                     /locus_tag="AC1PIW_001662"
                     /pseudo
     CDS             complement(2835..2948)
                     /locus_tag="AC1PIW_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936306.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylyltransferase domain-containing protein"
     gene            3555..3779
                     /locus_tag="AC1PIW_001663"
     CDS             3555..3779
                     /locus_tag="AC1PIW_001663"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606563.1"
                     /translation="MKVSTLERTSCFERKNQALEQKDNEVILVYIYMPCSAMLANMAS
                     TEKFTKKQVSKPNHLFFSKRTYKIGNKKTT"
     gene            complement(3890..4201)
                     /locus_tag="AC1PIW_001664"
     CDS             complement(3890..4201)
                     /locus_tag="AC1PIW_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606564.1"
                     /translation="MNPTNSRIALFDTIKCIMVLCVIFTHLDWSVEQRQWFIFPYFVD
                     MAVPIFLLLSAYFRTNKWNTKQETLKLKFSSGIKESINMLCLYAIVMAVNVLLSYSRT
                     I"
     gene            complement(4241..>5206)
                     /locus_tag="AC1PIW_001665"
     CDS             complement(4241..>5206)
                     /locus_tag="AC1PIW_001665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIP6606565.1"
                     /translation="EQYLSKCINSIVNQTYKHIEILLVNDGSTDNSEEICLAYAKKDS
                     RIRYFKKENGGLSDARNYGISRAKGDYLAFIDSDDFIHSEFIQRLHEAIERENALVAV
                     AGYDRVDASGHFLTAEPLPTNQAVLSGRNVCKKLLEADGHRFVVAWNKLYKKELFEDF
                     RFEKGKIHEDEYFTYRLLYELEKVAIVKECLYYYVDRENSIITSSMTDHRFHCLLEFQ
                     NERMDFYESRGDKELLLECYRSFLAFAVLFLGKYNHWLSKQQKKLLQTLFRIVYKQLK
                     QNKRLALLMNAYYLVGCLHLNFSVFLKTGKDKIQERLRRSESSTR"
BASE COUNT         1784 a         1128 c          865 g         1429 t
ORIGIN      
        1 tagaggtgcc cttatttaga ccgtttttcc ctgaatgtat tgagggcaac tgttaattcc
       61 tttctaaaaa tgtaggcata aacgactaga accgctattc ctagtgacca acgaatccag
      121 attgaaccga caaatactgt catcaagcct gtatagacga caacaagagc aattacctta
      181 acaaatgttg aaatcgcaac ttcatcgtaa gcatactttt tcttagcaac aaaataatgc
      241 aagactagca acaacagata ggaagcagtc gttgccaaag cagcaccata aattcctatc
      301 gtcggtatca aaacaaagtt gactgaaata tttagtacac ctgctataaa agtaccaatt
      361 ggcaaaaact ttgtatttcc actataaaac tggatattgg ctggaaaact ataaagaaat
      421 acaaagaaca ccccgacaat aatcatggga ataaatccca tactgaaacg atactcagat
      481 ccacctaaca acatcgctaa ttcagggtaa attgttagaa atccaaaagt cacaaacagg
      541 ccaatcgcca gatagtaacg gacataactg agcaaatctt tatctgcacc tctctttttc
      601 tcaaaatacc acggacacca taccgtattc aagctcgaaa acacaatttg taagatagac
      661 gcaagtgtgt agccgaaact gtatagggct acatctgaca gtgttagcat cttgccgagc
      721 atgattctgt caaattgatt gagtacatta tgacctaatc catgaaaaat aagaggaatc
      781 gatatactta aaccataccg aagatagtcc tttcgaaaat gaagcccaat cttcttatag
      841 aaaaacaaca aggacacaca agcaaaaaca ccagtcgttg ccgagtttgc cattacacga
      901 gcgatgaaat cattctccat cgaaaagatg agaaataaag ataaagcagt gttgataaca
      961 gcgctcagta ccgataggag taaagtccac atggactgct gccgctgcac taaataggtc
     1021 gtaaaaaatc cttgcacaac actcataaaa ctttgcaaaa agataagcgg aacgacccaa
     1081 tcaggcaaac caaataggag cgataggggc tgactgagga gaaaagatag cccaaaaatt
     1141 ggtaaaaaga aagcgataga agagaccatc aaggtggata cgaaatcatc aaatttctcg
     1201 cggaagtgta cccatcccgg gccaaaagcc ccacctaact gtagaccgat aaagagacca
     1261 actagcccca cccacgaatt atacaagcta aactgcccat atacttcctg agaaatgaca
     1321 cgagtataga tggggagggt aatgaaggct aatccctgaa cgaggatggt tccaatcgta
     1381 tagacgagac ctgagacaac tattgatttt ttagacatga atcctcctat tctatcccct
     1441 cacgcaaaat atcatttttt gaaagaagag gaaaagtgtc ctttaaatac actttctctt
     1501 caaaacaatt taaaaaatct aaagcagacg aactataggt cataccgata tcaaaacgga
     1561 tactacctgc catttcgaac aactcaaacg gaataccttg aggcagaagc accacagcct
     1621 tacccagaaa tgaataatca accccatctc gtgggtgcgg ttttatgtag attgtatagt
     1681 cttcccgata aggggcaaga cctgctacat aaatctccaa caactcctct tcggtcacat
     1741 gataatccca agacaagggc tgggtcaata atagcaatcg tttttgagaa gactcttcat
     1801 ccgctactat cgcccttgct ccaaatatct gcagcagcgc ttgcaccttc tctggcgatg
     1861 cttgatcaaa taattgcttt ctcggaactt ctacaaaggg tttataagcc tcgtcaaatt
     1921 gtacgagcga cagatcattg acctcaatgg attgacaata gagacttgaa ccaatcaaat
     1981 atgttggttt aaaataccat tgataaaaga gtcttttcca gacagatgat tgaattgact
     2041 ccaaatcgca cactctttta tccttgaaaa aattataacc atcctccaaa agagaatagg
     2101 gaatccgatg tttatttaaa aaatgaccga cttgacggtg atcttgaaaa atgatcacct
     2161 cgtcatagct ctgtaacaag gaaaggcgtt cttttgtttt tgatctagct atcaaagaat
     2221 atagatcaaa tgaacgctcc gtctcctcga ttagatgaac atgctgctgc agttgctccc
     2281 tgacatctgg aaaatgcccg atgatatcaa cggacatcaa actatctctc tcaacgtcca
     2341 tctttaacaa ggaaatcagg atctgataca tcgtatggca aatataaatt cgtctcaatt
     2401 tttctccttc tacatacaga ctttaaggat aagtaacccc tttttataac ccctgtacag
     2461 cttcgataat gtactgattc actctttctt gcccttcatc attgaaatgc aaaacatttt
     2521 ctgagtcccc ctgtaagtac atattcatgg cttcttttat ctgttcccca gtccgtaacg
     2581 gtgaggtgac ttgcagaaga acaaatatac aatcttcata aaatccagat tcgtaaaaaa
     2641 gtgcgtactg acttcattcg atggagtatg ataagtagat agttctttgg gtcttaacaa
     2701 tacattgatg cctcttgtct cgcaaatccc cttggggacc tctaaaaact aattttctaa
     2761 aatttaagat gttgatttaa caaggctttc gtaactccaa attcgcaatt ttgggaattt
     2821 atagaggtgc cccccttata catttctgaa tccgtactga catagatgtc ttctttctca
     2881 aaacaacctg attcaattgc cacatcaatc gtgtgaaaaa tcatgggttt cccgtccaaa
     2941 aatagcatgt ttttattcgg taagcctttc gaaccagatc gtgctgggat taaacaaaca
     3001 gttctcttaa aaattctcct tgttcagtta ataaatatag ctttgaaaat aaagggggat
     3061 actggggttt agaaacagat accactcctc tctcgtcaac ctatttgaat ttctttgtcg
     3121 gaatattaac atgcgaatct gctaataaac gaaatggcat ctgctaatct gaaatgagtc
     3181 attatcgttt tccacgtact ctcataaaca gatccacttt gtagaaaata tagccaccaa
     3241 acaggcaaat tagaaaggta aacaagccgt tccacaaatt caacgaagat accttaaatt
     3301 gcactgctaa aaatggtgcg actactgaaa aatacagcat ctgggtcaag tagatatgat
     3361 aagaagcaac tcccaatttt gacaacagta ttgctggaat cttctgtcca tactttatca
     3421 taaaaaatag catagcatac gcaaatggga cgcatagaaa ggaagtagac ttccaaccat
     3481 aaaaccagga gaagggctct atagaatgat tcacaaaaat cagaattgct ccaataatcc
     3541 caaatagggt cgcaatgaaa gtatctacct tggaacgcac atcctgcttt gaaagaaaaa
     3601 accaagccct agaacaaaaa gataacgaag tgatattagt ctatatatat atgccgtgtt
     3661 cagccatgtt agcaaatatg gcatccactg aaaagtttac taaaaaacaa gtaagcaagc
     3721 ccaaccattt atttttctcg aaaagaacat acaaaattgg taataaaaaa actacctgaa
     3781 tcaacaacgg aacatagtaa ctcccaactc cctggacccg attctccaga aagtagccac
     3841 aggacaaatg aacggagcga tgaagaacct gaaaaaggct ttactcctat cagatggttc
     3901 tcgaatagct caataaaaca ttaacagcca tcacgatagc atagagacaa agcatgttta
     3961 tactttcttt tataccactg ctgaacttga gctttagcgt ctcttgtttt gtattccact
     4021 tattcgttcg aaaataggca gaaagcaaca gaaaaattgg aacagccatg tcaacgaaat
     4081 acggaaagat aaaccattga cgctgctcaa cagaccaatc cagatgtgta aaaataacac
     4141 aaagtaccat gatacattta atcgtatcaa agagtgctat tctactattt gttggattca
     4201 ttttaatccc cttttctttc aaccatttat tacaacattc ttaccgagta ctactttcac
     4261 ttcttctcaa tctttcttga attttatctt tccccgtttt cagaaagaca ctaaaattaa
     4321 gatgaagaca ccctaccaaa taataagcat tcattagtaa agcaagtcgc ttattttgct
     4381 tcaattgttt atatacaatt ctaaatagcg tttggagaag cttcttttgc tgtttgctca
     4441 accaatgatt atatttgcct aaaaacaaaa cagcaaaggc taaaaatgaa cgataacact
     4501 ctagtaagag ctctttatct cctctacttt catagaagtc cattcgttca ttttgaaatt
     4561 ccagtaggca atggaagcga tggtcagtca tactagaagt tatgatacta ttttctcggt
     4621 caacataata gtacaagcac tccttaacta ttgcaacttt ttctaactca tagagcaagc
     4681 gataagtgaa gtattcatct tcatgaatct tacccttttc aaatcgaaaa tcttcaaata
     4741 gttctttttt atagagttta ttccaggcca ccacaaagcg atgaccatcc gcctctagca
     4801 gctttttaca aacattcctg ccgctcagaa cagcctgatt tgtaggaagc ggctctgctg
     4861 ttaagaaatg ccccgaagca tctaccctat cataaccagc aactgccaca agggcattct
     4921 ctctctcaat tgcttcgtgt aaacgttgga tgaactccga atgaataaaa tcatctgagt
     4981 ctataaaagc taagtagtca cccttggcgc gacttatgcc ataattacgg gcatctgata
     5041 gcccgccgtt ctctttttta aaataacgaa tgcgactatc tttcttcgca tatgctaaac
     5101 aaatttcttc cgaattatcc gtactaccgt cattcaccag aagaatctct atatgtttgt
     5161 aggtctgatt tacaatgcta tttatacact tggatagata ttgttc
//
LOCUS       DBNFCK010000118         5177 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.118, whole genome shotgun sequence.
ACCESSION   DBNFCK010000118 DBNFCK010000000
VERSION     DBNFCK010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5177
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.118"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            17..1093
                     /locus_tag="AC1PIW_001666"
     CDS             17..1093
                     /locus_tag="AC1PIW_001666"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIP6606566.1"
                     /translation="MAYVVAVVGATGAVGAQMIKMLEESTLPIEKVRFLASARSAGKT
                     LQFKGQDIVIEETTETAFEGVDIALFSAGGSTSAKYAPYAVKAGAVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLERIIVSTYQAVSGA
                     GMGAILETQAQLRSVLNDGVEPKAVEANILPSGGDKKHYPIGFNAIPQIDLFTENDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEIAPIDEVKAAIASF
                     PGAVLEDDVANQIYPQAINAVGSRDTFVGRIRKDLDKENGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            1166..2101
                     /gene="dapA"
                     /locus_tag="AC1PIW_001667"
     CDS             1166..2101
                     /gene="dapA"
                     /locus_tag="AC1PIW_001667"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIP6606567.1"
                     /translation="MSIQDLRDVKIITAMITPFKEDGSINFEVLPELIEHLLSHHTEG
                     ILLAGTTAESPTLTHEEELELFGAVQKIVNGRVPLIAGIGTNDTRDSIEFAKEVAAFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVEMTPETMLRLAE
                     HPNIIGVKECTSLANMAYLIEHKPEDFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMYEMFTAIEQQDIRTAAAIQRKFIPKVNALFSYPSPAPVKAVLNYLGFEVGPLRLPL
                     VPCPEEDAKRIIKVVVDGDYEATKATVTGVVRPDY"
     gene            2316..3668
                     /locus_tag="AC1PIW_001668"
     CDS             2316..3668
                     /locus_tag="AC1PIW_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIP6606568.1"
                     /translation="MNIVIIGASFAGLSAALECRKLYQNAQITLIDREKDVAYFPNTL
                     NWKVAGKISGWEEAKISLFSEVQHAAISCLFETECLAVYPEMRKVKIRHHQDIGDIIY
                     DRLILAMGASQTWEWGTEELQDYLIRSKTLSQAQASLEKLTNAKTVAVIGAGQIGLES
                     LDALSQLDLNLHVFEAQDSLLAKYVDEDMIAPIRQEMKKRGIQLHLSETVNQITIDTQ
                     EEQLVCHTVTGNYPADLVLPSTNLTPNIQLVKDRLKVIAEGTVWVDEFLETSQENIFA
                     VGDLVSLPVDYFGQAYLPMIHHAIMTGRMAARNLQKRIAPIKRAQRIVSSHVFGYYIT
                     SLGLTESEASLWLESKSIRIRRSYSQWEPTLVDFKLIVEETSGRIIGGQLVSTADYIE
                     QMDVLSLAISKDLTVFDLVEHSWLCLPGNTPLVPFIVEAAQEYIRQFYQVDGEGVYAD
                     "
     gene            3658..5121
                     /locus_tag="AC1PIW_001669"
     CDS             3658..5121
                     /locus_tag="AC1PIW_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIP6606569.1"
                     /translation="MRIDSLLEKRELAIYQLLLYLEDRKEAPSLKDICHYLELSKLTL
                     FRYIVSFNEEAEVTDLGLSFQLEDEKVFLKKKASLSLHRILDYLCQTSTKYQIIVYLS
                     DKEEVSIQQLAQELLISEATLNRHLASLNQILAEFGIGIKSGRLKGSELQIRYFLHQM
                     LSLTTTRAELQEEFMLGKIEALLPVFERFYQSPLNPKQAHRLLLWLMISQQRSRLRQL
                     DFKHLYKLMEPYQEHKFYRRLRKMYFTLSQQQSTSFQEGEIMALFAFLFSHFILVPHQ
                     LEQVLGFGGPIMEATSLALQKLRLYFEGNLPVSEEALYHLNQIMSQLYFFYSSVELEE
                     VEASSFYSEAEDLLKDVYKSVFHRKVDKKIRNSYLTNIADLYIYFGQVQPIQVKVAFA
                     SSLHEVLSYPLLLQLREKLEGNRQVLIEPYQAGYDYDLIITDYLENTSIPVYCLGNRL
                     KIQDIVQLKAWIQDIYHQKSCQSEIMATKTSFPIEHR"
BASE COUNT         1507 a          988 c         1160 g         1522 t
ORIGIN      
        1 cttaaaggag atttctatgg cctatgtagt agctgttgtt ggtgccactg gtgcagttgg
       61 tgcccaaatg attaaaatgt tggaagagtc aacacttcca atcgagaaag tgcgtttcct
      121 tgcttctgcc cgttcagcag gtaagacctt gcagtttaag gggcaggata ttgtcattga
      181 agaaacaaca gaaacagcct ttgaaggagt ggatattgcc ctcttctcag ctggtggctc
      241 tacatctgcc aagtacgctc cttacgctgt aaaagcagga gctgtagtag ttgacaatac
      301 ctcttacttc cgtcaaaatc cagatgttcc tctggttgtt cctgaggtaa atgctcacgc
      361 gcttgatgcc cacaatggta tcattgcttg tccaaactgc tcaaccattc aaatgatggt
      421 tgccttggag cctgttcgtc agaaatgggg cttggagcgg atcatcgtat ccacctatca
      481 ggcagtctct ggagcaggta tgggagctat cttggaaaca caagctcagc ttcgttctgt
      541 cttgaatgat ggcgtggaac ccaaagcggt agaagcgaat attcttcctt ctggtggcga
      601 taagaagcac tatccaatcg ggttcaatgc tattccgcaa atcgacctct tcactgaaaa
      661 tgactacaca tacgaagaga tgaagatgac aaaagagact aagaaaatca tggaagatga
      721 cagcattgcc gtttctgcaa cctgcgtccg tattccagtc ttgtcagctc actctgaatc
      781 tgtttacatt gaaactaagg aaattgcacc gattgatgaa gtgaaggcag ctattgcatc
      841 cttcccaggg gctgttttag aagacgatgt ggctaatcaa atttatccgc aagccattaa
      901 tgctgttggt agtcgcgata cttttgttgg tcgcatccgt aaagatttgg acaaggaaaa
      961 cggtattcac atgtgggttg tttctgacaa cttgctcaaa ggcgctgcat ggaactctgt
     1021 tcaaatcgct gaaacccttc atgagcgtgg tctagttcgt ccaacggcag aattgaagtt
     1081 tgaattgaaa taattttatt aaccaaaagt acacaaaagg ctgcactcag ccttttttag
     1141 caacaatcag taagagagga agcctatgtc tattcaagat ttacgtgatg taaaaattat
     1201 tacagcaatg attacccctt tcaaagaaga tggttcgatt aattttgaag ttttacccga
     1261 attgattgaa catctgttgt cccatcacac agaagggatt ttattagcag gaacaactgc
     1321 cgaaagtcca accctgacac acgaggaaga actggaacta tttggtgctg tgcaaaaaat
     1381 tgtcaatggt cgtgttcccc taattgcagg tatcggtaca aatgatacgc gcgactcgat
     1441 tgagtttgcc aaagaagtgg cggcattcgg cggctttgcg gcaggtcttg ctatcgtgcc
     1501 ttactacaac aaaccctctc aagagggaat gtatcagcac ttcaaggcta ttgcggatgc
     1561 ctctgatttg ccaatcatta tctacaatat cccaggtcgt gtcgtggttg aaatgacgcc
     1621 agaaaccatg ttgcgtttgg ctgagcatcc aaacatcatc ggtgttaaag agtgtaccag
     1681 ccttgctaac atggcttacc taattgagca taaaccagaa gacttcttga tttatacagg
     1741 tgaggatggc gatgctttcc atgcaatgaa cttgggagca gatggtgtga tttctgttgc
     1801 atctcacacg aatggtgatg agatgtatga aatgtttaca gccatcgaac aacaagatat
     1861 tcgaacagca gccgctattc aacgcaagtt cattccgaaa gtcaatgccc tcttctcata
     1921 tccaagtcct gcaccagtta aggcggttct taattacctt ggatttgaag ttggtccact
     1981 ccgcttgccg ttggttccat gcccagaaga agatgctaaa cgcattatca aagtcgtagt
     2041 ggacggcgac tacgaagcga ccaaagccac tgtgacagga gtcgtaagac cggattatta
     2101 aaatagtcag gggagtgact attttaacgt gagttagggc ataaagagtg aggtgtttag
     2161 aaaaatatta atctagagga gcttctggcc atcttcctcg ttttatttaa gtttacacaa
     2221 gcacatcagt tatctggtgt gcttttcagt tgcaagcctt ttcaattttt tggtcattct
     2281 tgatataatg actaaaaacg ataaaaggat taagtatgaa tattgttatt ataggcgcct
     2341 cctttgctgg tttaagtgca gctttagaat gtcgaaaatt gtatcagaat gctcaaataa
     2401 cactaattga tagagaaaaa gatgtggctt actttccaaa taccttgaat tggaaagtgg
     2461 caggaaaaat ttctggttgg gaagaagcga agatttcttt gttcagcgag gtgcagcatg
     2521 cagctatttc ttgtcttttt gaaacagaat gtctcgcagt ttatcccgaa atgcgaaagg
     2581 taaagatacg gcatcatcaa gatataggtg atattattta tgaccggcta atattggcta
     2641 tgggagctag tcaaacatgg gaatggggaa cggaagaact tcaagactat ctaattcgct
     2701 caaaaacgct atctcaagca caagctagtc tagagaaatt gacaaatgca aaaacagttg
     2761 cagtgattgg agcaggacag attggtttgg aaagcttgga tgcccttagc cagttggatt
     2821 tgaatttgca tgtgtttgag gcgcaagatt cactccttgc aaagtacgtt gatgaggata
     2881 tgattgcgcc gattcgtcaa gagatgaaaa aaagaggaat ccaacttcat ttatccgaaa
     2941 cagtcaatca gataacgatt gatactcagg aagaacagct ggtttgtcat acagtcacag
     3001 gtaattatcc agctgattta gtcttgccat ccaccaattt gacgcccaat attcagctag
     3061 tgaaagacag gctaaaagtg attgcagaag ggactgtatg ggtggatgag tttcttgaaa
     3121 caagtcaaga gaatattttt gctgtaggtg atttggtcag tctcccagta gattattttg
     3181 gacaggcata tcttccgatg attcatcatg cgattatgac agggaggatg gctgctcgaa
     3241 atttgcaaaa gaggattgct cctattaaga gagcccagcg gattgtttct agtcatgtat
     3301 ttggctatta tataactagc cttggcttga ccgagagtga ggcgagcttg tggttggaga
     3361 gtaaatcgat tcggattaga aggtcgtata gccagtggga accaacttta gtagatttca
     3421 agctgattgt cgaagaaacg agtgggcgca tcataggggg gcaactcgtt tctactgcag
     3481 attatattga acagatggat gtactgtctc tagcgatttc aaaggatttg accgtctttg
     3541 acttggtaga acattcttgg ctctgtcttc ctggaaatac tcctctggtc cccttcatcg
     3601 tagaggccgc tcaagagtat attcggcaat tttatcaggt tgatggggag ggtgtctatg
     3661 cggattgatt ccttattgga aaagcgtgag ctcgccattt atcagcttct cctctactta
     3721 gaagatagga aagaggcgcc atcgctaaag gatatttgtc actatttaga attgtcaaaa
     3781 ttaactcttt ttcgctatat tgtatctttc aatgaagaag cagaagtaac cgacttggga
     3841 ctctcttttc aactcgaaga tgaaaaggtc tttttgaaga aaaaggccag tttgagtctt
     3901 catcgtatcc tagactatct ctgtcaaacc agtaccaaat atcaaattat tgtttattta
     3961 tctgataagg aagaggtttc tattcaacag ctggctcaag aattgctgat aagcgaagcg
     4021 actctgaatc gccatcttgc ctctttgaac cagatattgg cagaatttgg tattggaatc
     4081 aaaagtggtc gtctaaaagg tagtgagctt caaatacggt actttttaca tcaaatgttg
     4141 agtttgacta ctacgagagc ggaattacaa gaagagttta tgctagggaa aatagaggcc
     4201 ttattgccgg ttttcgaacg cttctatcaa tcgccgctaa atcccaagca agcccatcgt
     4261 ctgttgttgt ggttaatgat ttcacagcaa cgctctcgat tgcgacagct agattttaag
     4321 cacctttaca aattgatgga accctatcaa gaacataagt tttaccgacg tttgagaaag
     4381 atgtatttta cactaagtca gcaacaatcc acctctttcc aagaagggga aattatggcg
     4441 ttgtttgctt tcctgtttag ccattttatt ttagtgccgc atcaattaga gcaggttctt
     4501 ggtttcggtg gaccgattat ggaagctacc tctttggctc ttcagaaatt acggttatat
     4561 tttgagggaa atttaccagt ctcagaagaa gcgctctatc atttgaacca aatcatgagc
     4621 cagctatatt tcttttattc ttccgttgaa ctggaagagg tagaagcttc tagtttctat
     4681 tcagaagcag aagacttgct aaaagacgtc tataaatcag tttttcatag aaaagtcgat
     4741 aaaaaaataa ggaactctta tctgacaaat attgcggatt tgtatattta ttttggtcag
     4801 gtccagccta tacaggtcaa ggttgctttt gcttcttccc tacatgaggt tctatcctat
     4861 cctctcttgt tgcaactaag ggagaagtta gaaggcaatc gtcaggttct tattgagccc
     4921 tatcaagcag gatatgacta tgatttgatt attacagatt acctcgaaaa tacaagcatt
     4981 ccagtttatt gcctaggaaa tcgattgaaa atccaagata tcgtacaatt aaaggcatgg
     5041 atacaggata tatatcatca aaagtcctgt caatcagaga ttatggcgac caaaacttct
     5101 tttcctatcg agcatcgcta gatgatgtca gatagtcttt gtgaaaaatg taagtgcatt
     5161 taaccttgaa aaattta
//
LOCUS       DBNFCK010000119         5016 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.119, whole genome shotgun sequence.
ACCESSION   DBNFCK010000119 DBNFCK010000000
VERSION     DBNFCK010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5016
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.119"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(225..1022)
                     /locus_tag="AC1PIW_001670"
     CDS             complement(225..1022)
                     /locus_tag="AC1PIW_001670"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HIP6606570.1"
                     /translation="MNQISISNLEFSYHPDKKILKGLNIILDERSTAIIGQNGAGKTT
                     FVKLLKGLIRPTAGKIMLNGQDLSEQSVAKIAKSIGLIFQNPDDQIFKHTVLDEIMFG
                     PLQMGMTHSQAEQAARKTMSLLGLDGFDKVNPYDLGLSDRKMVSIAAIVAMDTQVVIF
                     DEPTIAQDDAGKKQIQSVIQLLREQGKIVISILHDMDFVAETFERALVFAQGEVLLDG
                     SVREVFSQKDVLEKAYLSQPTTMQLCQALGYDDVFLTPEEFIAYKQE"
     gene            complement(1019..1873)
                     /locus_tag="AC1PIW_001671"
     CDS             complement(1019..1873)
                     /locus_tag="AC1PIW_001671"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HIP6606571.1"
                     /translation="MAHIKIENLKYQYPFAEKLALDGITCEIRPGEFIGVIGRNGSGK
                     STFCQSLTGLVPNFYRGSYGGKVWIDQSEVKRVEVDDLCQKVGSVFQNPFNQITGSKA
                     TVYEEIAFGLENFGVPKEEMEVRIEESLRLLDIEEYRDRAPFDLSGGQMQRMAIASIM
                     AMKPEVIILDEPTSQLDPQGSEAVFKAIQSLSKQGITVIMVEHKVEKIAAYSDRVMLL
                     DQGKLIAMDTPQAVFSRPDLEKYGVVAPTFTRICKQLGMKLPGSSYYPVTLEEASQLL
                     AKNRGGNV"
     gene            complement(1873..2637)
                     /locus_tag="AC1PIW_001672"
     CDS             complement(1873..2637)
                     /locus_tag="AC1PIW_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HIP6606572.1"
                     /translation="MKTMSLFEDKNSFLNNLSPLTKMLYVLATILIPIIAGDLAVFSI
                     TILLSVVLLAHSKVLRQTLPILSVSGFVILTVGIIQGLFRSGNVTPVFQLGPVIFYKE
                     GLIFAAEIALNVCNIILAFCVLILTTKSSDMIEELVRKGFSPRMGYVFVSLFQLIPQM
                     TSRMATITDAQRSRGMETEGSMLVRLKAFLPLVSPVIMSSFIDTRERAIALEVRGFNS
                     KSKKTFINPPIRSQFDRLIQIFCIFGVVLAIVWRVL"
     gene            complement(2648..3229)
                     /locus_tag="AC1PIW_001673"
     CDS             complement(2648..3229)
                     /locus_tag="AC1PIW_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIP6606573.1"
                     /translation="MKFNTAALVLIPAGVGINYIGKLIAGVLKLPLWLDSIGTCLSAC
                     LAGPIVGAVVGIMNNLIYGLTVDPISTVYALTNAALGIAVGLLAYYGSMKNIRGALFT
                     GIVAGFAAVLVSTPLNMIFWGGTTGNLWGDLVYAGLLAQKFPVWFASFMDEIVVDIPD
                     KIVVLLLVLSLYKSLPKSLLSLYQSNSVIKNLD"
     gene            complement(3264..4010)
                     /locus_tag="AC1PIW_001674"
     CDS             complement(3264..4010)
                     /locus_tag="AC1PIW_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HIP6606574.1"
                     /translation="MVLQPHIRCDIEQGARYAILPGDPQRVDRVAEFLDSVEFIAFNR
                     EHKTMRGSYKGVPVLVTSTGMGGASTGIAVEELARIGVECMIRIGSCGALQPEMQMGD
                     LVILNGAVRDEGTSRAYVDPIFPAVPDPDVLFALRGTAMNQGFAHHVGIARSHDSFYI
                     DNELEVDHFWSKKGVLAGDMESAALFTIGSLRGIKTGSILNTVALYDGELKDEINQYV
                     SGENRVTQGEKNEILTTLEAIVMLEEQRKG"
     gene            complement(4022..4696)
                     /locus_tag="AC1PIW_001675"
     CDS             complement(4022..4696)
                     /locus_tag="AC1PIW_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIP6606575.1"
                     /translation="MILKMHLLLDIIEMNPDVYSLLKLCPYEILRKIHVKRFEDGEFE
                     LEQGEIYQYMYIIVEGRVNVYSESENGKKYFLCCSQSGDLIGEMEIFQQVPYISYVKG
                     VGDIIALEIDKDTLLSWMALDQNFSSLFIKKICENSYRMCNRMKRNSLYDLRQRLAYY
                     LLEQFGGKKLASLQFNSERLSQEMAVTQRSINRLLKEFKEKQIIDLGHGKIQLLDHQA
                     LTSLLD"
BASE COUNT         1591 a         1145 c          726 g         1554 t
ORIGIN      
        1 ttttctgatt caaattttta atttacaaat ttatagtgat acagaaagga gaacctcgtc
       61 cgcttcaaaa agattaaaag aatagaaaca gaactacctc aatctgcaat ctctcacaaa
      121 cttagaatta gactaatatc gttcaataat taggaagcag tatagaataa cattataaaa
      181 accaagcggt taccaggaat ctcctagatt gtaatttaag tacatcactc ctgtttgtaa
      241 gctatgaact cttcgggagt gagaaaaacg tcatcatagc ctagtgcttg acataattgc
      301 atcgtggttg gctgagatag ataggctttt tcaaggacat ctttctgtga aaaaacctct
      361 cgaacacttc catctaacaa aacttctcct tgtgcaaaga caagggcacg ttcaaacgtt
      421 tctgctacga agtccatatc atgcaaaatg gaaataacta tttttccttg ttctctcaac
      481 aattgtatca ccgattgaat ctgttttttt cctgcatcat cttgagcaat cgttggttca
      541 tcaaaaatca caacctgtgt atccatagca actattgcag cgattgacac catttttcta
      601 tcggataatc ctaagtcgta gggatttacc ttgtcaaatc catctaagcc cagcaaggac
      661 atagtttttc tagctgcctg ttctgcttga gaatgagtca tgcccatttg caatggtcca
      721 aacataattt catctaatac cgtatgttta aaaatttggt catctggatt ttgaaaaatg
      781 agtccaatac ttttagcaat tttagcgaca gattgttcgc ttaaatcttg tccatttaac
      841 ataattttac cagccgtcgg acgaataagg cctttcagta actttacaaa tgtagtcttt
      901 ccagcaccat tttgtccaat aatcgccgtc gaacgctcat ctaaaataat attcaaccct
      961 tttaatattt ttttatccgg atgataggaa aattctaagt tactaatact aatttgattc
     1021 atacatttcc ccccctattc tttgccaaca actgactagc ttcctcaagt gtcacaggat
     1081 aataggaact tcctggtagc ttcatgccta actgcttgca tattctagta aaggtaggtg
     1141 ctacaactcc atacttttct agatctggtc gagaaaacac ggcttgtggt gtatccatgg
     1201 caattagttt tccctgatca agcaacatta cccggtcact gtaagctgcg attttttcaa
     1261 ccttatgttc caccataatt acagtgatgc cttgcttact taaagactga atagctttga
     1321 aaactgcctc actaccttgt ggatcgagtt gagaagttgg ctcatctaaa ataatgacct
     1381 ctggtttcat cgccataatg ctagcaattg ccattcgttg catttgtcct cctgacaagt
     1441 caaacggagc tcgatcacgg tactcttcaa tgtccaacaa acgtaaactt tcctcaattc
     1501 gaacttccat ttcttctttt gggactccga aattctccaa accgaaagca atttcttcat
     1561 acacagtcgc cttagaacct gtaatttgat taaatggatt ttgaaaaacg cttccgactt
     1621 tttggcacaa gtcatcaacc tctaccctct tcacttcgga ttgatcaatc catacctttc
     1681 ctccatacga accacggtaa aaattcggaa caagtcctgt taatgattga cagaatgtcg
     1741 attttccaga accatttcga ccaattaccc caatgaattc acctggacga atctcacacg
     1801 tgatgccgtc tagagccaac ttttcagcaa agggatattg gtattttaga ttttcaattt
     1861 taatatgtgc cattatagaa ccctccagac aattgctagc acaactccaa aaatgcagaa
     1921 aatttgaatc aatcgatcaa attgcgaacg aatcggtgga ttgataaatg tctttttaga
     1981 ttttgaatta aatcctcgaa cctctaaggc aattgcgcgt tctctcgtat caataaaaga
     2041 actcataatt acaggagaga ccaatggcaa aaaagctttt agccgaacca acatcgaacc
     2101 ttctgtttcc atcccacgac ttctttgtgc atccgtaatt gttgccattc ggctcgtcat
     2161 ctgagggatt aattggaaca aggagacaaa cacatatccc attcgaggtg agaagccttt
     2221 tctaactaat tcttcaatca tgtccgacga ttttgtcgtt aaaatcagaa cacagaaagc
     2281 taaaataata ttacatacat ttaaagcaat ctcagctgca aatatcaagc cttccttata
     2341 aaaaatgact ggacctaatt gaaaaacagg cgtgacatta ccacttctaa acaatccttg
     2401 aataattcct accgttaaaa taacaaaccc agatacactc agaatgggta aggtctgcct
     2461 taacacttta gaatgtgcta acaaaacgac acttaaaaga attgtaatgc taaatacagc
     2521 taaatcacct gcaataatcg gtatcagaat ggtcgcaagc acatacaaca tcttagttaa
     2581 cggagacaaa ttattcaaaa atgaattctt gtcttcaaac aaactcatcg ttttcatgga
     2641 gacctcctta atctaggttc ttaatcacac tattactttg gtaaagcgat agtagactct
     2701 ttggtaaact cttatacaag cttagcacaa gtaataatac gactatttta tctggaatat
     2761 ctaccactat ttcatccata aacgatgcaa accaaactgg gaatttttga gctaatagac
     2821 ctgcataaac caagtctccc cataaatttc cggttgtacc accccaaaag atcatattca
     2881 atggcgttga taccaagact gctgcaaatc cagcaacaat tcctgtaaat aaggcacccc
     2941 tgatgttctt catactaccg taataagcca ataaaccgac tgcaattcct agggctgcat
     3001 tggttaaggc ataaactgta gaaatcggat ccaccgttaa tccataaatt aaattattca
     3061 taattcccac aacagcacct acaattggac cagccaaaca agccgataaa caagttccaa
     3121 tcgaatctaa ccaaagtggt aatttgagaa cccccgcaat caatttacca atataattaa
     3181 tcccaacccc tgccggaatc aacactaaag ctgcagtatt aaacttcaat gataatggat
     3241 tattatttct ccaattcatg attctatcct ttcctttgtt cttctagcat cacaatcgct
     3301 tccaatgtcg taagaatttc atttttttca ccttgtgtca cacgattttc gccagacaca
     3361 tattggttga tctcatcttt caattcacca tcatatagtg ccactgtatt taaaatggag
     3421 cccgttttaa tcccacgtag gctaccaata gtaaataaag ctgcactttc catgtctcca
     3481 gctaatacac cttttttaga ccaaaaatga tcaacttcca actcattatc aatataaaaa
     3541 ctatcatggc ttctagcaat tccaacgtga tgggcaaaac cttggttcat agcagttcct
     3601 cgtaaggcaa ataaaacatc agggtctgga accgccggaa aaattggatc aacatacgcc
     3661 ctagaagtcc cctcatcacg tacagcccca ttcaaaatga ccaaatctcc catctgcatc
     3721 tccggttgca atgctccaca tgagccaatc cgaatcatac attccacacc gatacgtgcc
     3781 aactcttcta ccgcgattcc tgttgaagca ccccccatcc ctgtagaggt aactaacact
     3841 ggaaccccct tatagcttcc acgcattgtt ttgtgttcac gattgaatgc tataaattct
     3901 acactatcta aaaactctgc tactctgtcc accctctgtg gatcgcccgg caatatggcg
     3961 tatcttgcac cttgttctat gtcacaacga atatgtggtt gtagtaccat ctttctttct
     4021 cctaatctag taaactcgtt aaagcttggt ggtccaatag ttgaatctta ccatgaccta
     4081 aatcgatgat ttgcttttct ttaaattctt ttaacaatcg attgatactc cgttgggtaa
     4141 ccgccatctc ttgactcaat ctttccgaat taaactgtaa tgatgctaat ttttttcctc
     4201 caaattgctc cagcaaatag tatgcaagtc gctgtctaag atcatataga ctattgcgct
     4261 tcatacgatt acacatacga taggaatttt cacaaatctt cttaataaac aaactactaa
     4321 agttttgatc taatgccatc caactcaaaa gtgtatcttt atctatttcc aaagcgataa
     4381 tatcaccaac tcctttcaca taactaatat acggaacttg ttgaaatatt tccatttctc
     4441 caatcaagtc accagactga ctacaacaaa gaaaatattt tttcccattt tccgattccg
     4501 agtaaacatt gaccctcccc tcaacaataa tatacatata ctgatagatc tccccttgct
     4561 ccaactcaaa ttcaccatct tcgaatctct tgacatgtat tttacgcaag atttcatatg
     4621 ggcaaagctt tagtaatgaa tatacatctg gattcatctc gattatatcc aatagtagat
     4681 gcattttcaa aatcatacac gctcctttct ttattgttac cacaacaatg ataacgcttt
     4741 cttttttgca agtaaaggaa ttccgtccga ttcaatagca aaaaaacata tccaatgtca
     4801 taatacacaa acattttaag gacaaatgtc ttcttgtttt acgatttgtt ccagatttct
     4861 aagccatact ttataaataa attttgataa aatcttccta attccaagag cttttaaact
     4921 ctaatttgta ttacttaact tctactcatt tcgaatgatg aatgtttgta ttgctaaatg
     4981 cggattccat tacaaaagat gatttaattt tttgtg
//
LOCUS       DBNFCK010000120         4851 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.120, whole genome shotgun sequence.
ACCESSION   DBNFCK010000120 DBNFCK010000000
VERSION     DBNFCK010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.120"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            228..2879
                     /gene="adhE"
                     /locus_tag="AC1PIW_001676"
                     /gene_synonym="adhC"
     CDS             228..2879
                     /gene="adhE"
                     /locus_tag="AC1PIW_001676"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HIP6606576.1"
                     /translation="MADKKVVSPEEKLVEARKHVDELVQKGLVALDEFRKLGQEEVDY
                     IVAKASVAALDKHGELAMHAFEETKRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLIALKTRNPIVFAFHPSAQESSAHA
                     ARVVYEAAVAAGAPENCIQWVTKPSMEATSELMKHDGIATILATGGNAMVRAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEVYDEFVAE
                     FKSYKTYFVNKKEKALLEEYCFGVKANSKNCAEGKLNADIVGRPAAWIAEQAGFSVPE
                     GTNILAAEVAEIGEKEPLTREKLSPVIAVLKVEGRAEGLEAARQMVEFHGLGHSAAIH
                     TEDAELAKEFGTIVRAIRVIWNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSISDNVS
                     AMNLLNIKKVGRRRNNMQWFKVPSKTYFERDSIQYLQKCRDVERVMIVTDRAMVELGF
                     LDRIIEQLDLRRNKVVYQIFSDVEPDPDITTVYKGTELMRTFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPTVDFHDLVQKFMDIRKRAFKFPELGKKSKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPAHVTADTGMDVLTHATEAYVSTVA
                     NDFTDGLALQAIKLVFENLESSVKNADFVSREKMHNASTMAGMAFANAFLGISHSMAH
                     KIGGRFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYKADEKYQDIARLLGLPC
                     STPEEAVAAYAQAVYDLGERIGIQMNLKAQGIDEKELKEHSRELALLAYEDQCTPANP
                     RLAMVDHMQEIIEDAYYGYKERPGRIK"
     gene            3293..4777
                     /gene="thrC"
                     /locus_tag="AC1PIW_001677"
     CDS             3293..4777
                     /gene="thrC"
                     /locus_tag="AC1PIW_001677"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIP6606577.1"
                     /translation="MTLVYQSTRDAKNTVSASQAILQGLATDGGLFTPLSIPTVDLDF
                     SVLKDASYQDVAKLILSAFLDDFTADELDYCINNAYDSKFDTSVIAPVVKLKGQYNLE
                     LFRGSTIAFKDMALSILPYLMATATKKHGLENEIVILTATSGDTGKAAMAGFADVPGT
                     QIIVFYPRDGVSKVQELQMTTQTGANTHVVAIDGNFDDAQTNVKHMFNDEALRAKLAA
                     KKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIAAGDKVNFTVPTGNFGNILAAY
                     YAKQIGLPVGKLICASNDNNVLTDFFATGVYDKNRTFRVTTSPSMDILVSSNLERLIF
                     HLFGNDAAKTAELMEALNTAGQYDIQGADAEILSLFAAAFATEEETAAEIKRVYDESD
                     YIEDPHTAVASAVYKQYVEQTGDQTPTVIASTASPYKFPVVAVEAVTGQSGLSDFEAL
                     AKLHEISGVDLPPAVDGLETAPVRHNTVVAAADMQTEVERYLGV"
BASE COUNT         1420 a         1054 c         1071 g         1306 t
ORIGIN      
        1 aagaataaat gataaagatt tgtcttactt tatgtatttt tcgaccaaac aaagcagaaa
       61 caaatctgaa cgttttctga aaacaggaaa aagcaagcgc tttccaaaac aaaacagaaa
      121 aaatgtgaaa aaattaacaa tttctcttta caaataaaat aaaaaatagt agaataacat
      181 tgtgaaaaag ttaacaaata gttagcaagt atatttggag gaacacaatg gctgataaaa
      241 aagtagtatc accagaagaa aagttggtag aagcgcgcaa gcacgtcgat gagcttgttc
      301 aaaaaggttt ggttgctctt gatgagttcc gtaaacttgg tcaagaagaa gtagactata
      361 ttgtagcgaa agcttcggtt gcggcccttg ataaacatgg cgaattggca atgcacgcct
      421 ttgaagaaac caaacgtggt gtattcgaag acaaggctac taagaacttg tttgcctgtg
      481 agcacgttgt caataacatg cgccatacaa aaacagttgg tgtcatcgag gaagatgatg
      541 taacaggttt gactttgatt gctgagccgg tcggtgttgt ttgtggtatc acaccaacaa
      601 caaacccaac ttctacagca atctttaaat cattgattgc cttgaagaca cgtaatccaa
      661 ttgtctttgc cttccaccca tctgcgcaag aatcttctgc tcatgcagct cgtgttgtct
      721 atgaagcagc tgtggcagct ggtgcccctg aaaactgtat ccagtgggta acaaaaccat
      781 ctatggaagc aacgtctgag ttgatgaaac atgatggtat tgcaacaatc cttgcaactg
      841 gtggtaacgc aatggtgcgt gcagcttact cttgtggtaa acctgccctt ggtgtaggtg
      901 caggtaacgt tccagcctat gttgaaaaat cagcaaacat ccgtcaggca gcacatgaca
      961 tcgttatgtc taagtcattt gacaacggta tggtctgtgc gtcagaacaa gcggttatca
     1021 ttgataaaga agtttacgat gagtttgtag cagaattcaa atcttacaaa acttacttcg
     1081 ttaataagaa agaaaaagct cttcttgaag aatactgctt cggtgtgaaa gctaacagca
     1141 aaaactgtgc tgaaggtaaa ttgaacgcag acatcgttgg tcgcccagct gcatggatcg
     1201 ctgagcaggc cggcttctca gtaccagaag gtacaaacat cttggcagct gaggttgctg
     1261 aaatcggtga gaaagaacca ttgactcgtg agaaattgtc acctgttatt gccgtcttga
     1321 aagttgaagg tcgtgcagaa ggtcttgaag cagctcgcca aatggttgaa ttccacggtc
     1381 tcggtcactc tgcagctatt catacggaag acgcagaatt ggcgaaagag tttggtacaa
     1441 tcgtacgtgc tatccgtgtt atttggaact ctccatctac tttcggtggt atcggtgatg
     1501 tttacaacgc attcttgcca tcattgacac ttggttgtgg ttcttacgga cgcaactcaa
     1561 tcagtgataa cgtaagtgct atgaacttgc tcaacatcaa gaaagtagga agacgtagaa
     1621 ataatatgca atggtttaaa gttccttcta aaacatactt cgaacgtgac tctatccaat
     1681 atctccaaaa atgccgtgac gttgaacgtg tcatgatcgt tacagaccgt gccatggtag
     1741 aacttggatt cctagatcgt atcatcgaac aattggacct tcgtcgtaat aaagttgttt
     1801 atcaaatctt ctcagacgtt gagccagatc cagacatcac aacggtttat aaaggtacag
     1861 aattgatgcg taccttcaaa ccagatacaa tcatcgcact cggtggtggt tcaccaatgg
     1921 atgctgccaa agttatgtgg ctcttctacg aacaaccaac agttgatttc catgacctag
     1981 ttcaaaaatt catggatatc cgtaaacgtg ccttcaagtt cccagaactt ggtaagaaat
     2041 ctaagtttat cgcgattcca actacctcag gtacaggttc agaagtaaca ccgttcgccg
     2101 taatctctga taaagcaaac aaccgtaaat acccaatcgc tgactactca ttgacaccaa
     2161 ctgtggcaat cgttgaccca gcactcgtat tgacagtacc tgcacacgtt acagcagata
     2221 ctggtatgga cgtattgact cacgctacag aagcatacgt ttcaacagta gctaacgact
     2281 ttacagatgg tttagcactt caagctatca aactagtatt tgaaaacctt gaaagctcag
     2341 tgaagaatgc tgactttgta tcacgtgaga aaatgcacaa cgcatcaact atggcaggta
     2401 tggccttcgc caacgcattc ctaggtattt ctcactcaat ggctcataag attggtggac
     2461 gtttccatac cgttcacggt cgtacaaacg ctatcttgct tccatacgtt atccgctaca
     2521 acggtactcg tcctgccaag acagctacat ggcctaagta caactactat aaagcagatg
     2581 aaaaatacca agacatcgca cgtcttctag gcttgccatg ctcaacacca gaagaagctg
     2641 ttgcagcata cgcacaagca gtttacgacc tcggtgaacg catcggtatc caaatgaatt
     2701 tgaaagcaca aggtatcgac gagaaagaac tcaaagaaca ctctcgcgaa ttggctcttc
     2761 ttgcctacga agatcaatgt accccagcta acccacgtct agcaatggtt gaccacatgc
     2821 aagaaatcat cgaggatgcc tactacggtt acaaagaacg tccaggtcgc atcaagtaag
     2881 gattttcgtc acgatacatc gttaaagctc cttgcctaac tttagttatg cctacgtcgc
     2941 tttgccttgt ctcgtttaga aaatcattcc ttagtgattt agattcgcct tcgtcaatct
     3001 gcctagaaag gcttaggact agaaaaagtc taggtaggag tagtaaatcg tatcctactc
     3061 agttgaacgg agtaggattt ataggtaaat tgcctccaaa tatcgtaaga caatcctcta
     3121 ttgaaaaata ggggattgtt tgtttagaaa taatggtgga gattctgtaa aaagcgaaag
     3181 tggttggaaa gttagggttt agccgagaaa aagagacttt tctatctatc tttcacaatt
     3241 ttctgtcaat ttgtggtaga atagaaaaaa tagatttttt atgagggata ccatgacatt
     3301 agtatatcaa tcaacacgcg atgctaaaaa tactgtatcg gctagtcaag cgattttgca
     3361 gggcttggcg accgacggtg gtttgtttac accgctttct attccaacag ttgacttgga
     3421 tttttctgtt ttgaaagatg cttcttatca agacgttgcc aagctgattt tgtcggcttt
     3481 cttggatgat tttacggcag atgagttgga ctactgtatc aataacgcct acgacagtaa
     3541 gtttgacacg tcagttattg ccccagtggt taagctaaaa ggtcagtaca acttggagct
     3601 cttccgtggc tcaaccattg ccttcaagga tatggctctg tccatcctcc cttacttgat
     3661 ggcgacggcg actaaaaagc acggtttgga aaacgaaatt gtcatcctga ccgcgacttc
     3721 aggcgataca ggcaaggcag ccatggctgg ttttgcggac gttcctggca cacaaatcat
     3781 tgtcttctat ccgcgtgacg gggtatctaa ggtccaagaa ttgcagatga ccacgcagac
     3841 aggggctaat acccacgtgg tggctattga cggtaacttc gacgatgccc agaccaatgt
     3901 caaacacatg ttcaacgatg aggcactccg tgccaagttg gcggctaaga aactccagtt
     3961 ttcatctgcc aactctatga acatcggccg cttggtgcca cagattgttt actatgtcta
     4021 tgcctacgct cagctggtta agactggtga gattgcggcg ggcgacaagg tcaacttcac
     4081 cgttccaaca ggaaactttg gaaacatctt ggcagcttat tacgccaaac aaatcggtct
     4141 gccagttggc aagctcatct gtgcgtccaa cgacaacaat gtcctaaccg acttcttcgc
     4201 gacaggcgtt tacgataaga accgtacctt ccgcgtgacc accagtccgt ccatggacat
     4261 cttggtgtct tctaacttgg agcgcttgat tttccatctc tttggcaatg acgctgcaaa
     4321 aacagctgag ctgatggaag ccttgaacac agctggtcag tatgacattc aaggggctga
     4381 cgcggaaatc ctctcgctct ttgctgccgc ctttgcgacg gaagaagaaa ccgctgcgga
     4441 aatcaagcgt gtctatgacg agtcagacta cattgaagat ccacatacgg ctgttgcctc
     4501 agctgtctat aaacagtatg tggagcaaac aggggaccaa actccgacag tgattgcttc
     4561 tacagcaagt ccttacaagt tccctgtggt tgcggtagag gcggtgacag gccagtcagg
     4621 tttgtctgac tttgaagccc ttgcaaaatt gcatgagatt tcaggtgtag acttgccacc
     4681 agctgtggac ggcttggaaa cggcacccgt ccgccacaat accgtcgttg cagctgccga
     4741 tatgcagact gaagtagaac gctatttggg tgtttaattt cgatttattt tatgacaaac
     4801 tgctcatttt gaacagtttg ttttcttttt gtggtttaaa ttaaaattta c
//
LOCUS       DBNFCK010000121         4719 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.121, whole genome shotgun sequence.
ACCESSION   DBNFCK010000121 DBNFCK010000000
VERSION     DBNFCK010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.121"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..524
                     /locus_tag="AC1PIW_001678"
     CDS             <1..524
                     /locus_tag="AC1PIW_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HIP6606578.1"
                     /translation="YHSISDEDEEHVDELYKKMYRNLEYATISFNVAVSLTLLNLVLG
                     FGVTFFEDGAIMYGSIFDVVFYVILLISQIFIMKLTQKIRDYKLSAFATVKEMKDFAE
                     AMDEGEKQANYEMSFQIVFTLNQIVLPGLYLFLFVLSMLLQERQITAFLVVAFLHIYI
                     NVMQVRMIRRYFK"
     gene            546..752
                     /locus_tag="AC1PIW_001679"
     CDS             546..752
                     /locus_tag="AC1PIW_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606579.1"
                     /translation="MKENKKFISVLKIVYFVGLIGLGLYAVGYIFGEWLATGQIDLSN
                     LNILLPMVLGLPALLLFKKESDKN"
     gene            778..1467
                     /locus_tag="AC1PIW_001680"
     CDS             778..1467
                     /locus_tag="AC1PIW_001680"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIP6606580.1"
                     /translation="MNRLGNVKDWNVVKNWKFLLVGLAVVALNVLTQLAMFALPNFDG
                     NNLLIAAVLLLLAVVLGLILTGKLGLWKGEQKWGMVANVGFVVLAFIVMFGLKIIGGQ
                     LIMLEEGYGQTTANQEIINNSGLPTLVLFLFAVFFAPVLEELLFRGILMGRVFGKDSI
                     VGLLLSSFLFGLIHNPTNIGSWVVYGGMGLVLGLAYRISGKYTNALILHSLNNLLGFL
                     LMLLMQSLGLI"
     gene            1477..1794
                     /locus_tag="AC1PIW_001681"
     CDS             1477..1794
                     /locus_tag="AC1PIW_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606581.1"
                     /translation="MKLFLWILVGFLFFNSLSDRKKVKQLQARVKKLQKTTKGENQMS
                     VLLKELIGSKAKIRFDEEFSIAYEYTILAVDEEWVKISRELANGEIETKLVRVDNIID
                     LSF"
     gene            1804..2478
                     /locus_tag="AC1PIW_001682"
     CDS             1804..2478
                     /locus_tag="AC1PIW_001682"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIP6606582.1"
                     /translation="MLKFIKHVALLFLYFVAYQIASGFLMVGPTLQSIPDIPAQLIDS
                     TIWICAIIGLVLSIALIILLWKYIYPRHSVAYRVTASWFHKIQWPILLYIAFFIIQFI
                     VPVPESENQKLVIEFVSAYPLIAFSSVVIFAPILEELIFRGFFATYFFPKMADMKAVG
                     IYLFVTGALFSLVHMPATLPQFLIYFTMGLNLGWLYLIRRDIRYPIALHMLNNGISYL
                     MILFLV"
     gene            2586..3713
                     /locus_tag="AC1PIW_001683"
     CDS             2586..3713
                     /locus_tag="AC1PIW_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HIP6606583.1"
                     /translation="MFHFTTLFIGMDVHKESFSLCYYDMMANQFKHSTKVGPNVSYIV
                     NYVNELRRLYGQDVEVLCGYEAGCLGFTLYHQLQAHGIPCIVMAPTTVMKEGSKRVKT
                     DKKDAAQLTKALAFHSYRPVHISTVEDEQVKEYICMRTDHKVALKKIKQQILAFCLRH
                     DFRYTEGSSNWTQKHVRWLCPLNIDGLYAEILTEYLLTYEKLVDQIERYDARIEQLGQ
                     SDSYQEKVSQLFCFIGIKTLTALSIVTEIGGFNRFATAQHFDFASYLGLTPSENSSGD
                     RERRGAITKTGNSHVRRLLIEAAQSLAKGTIGYKSKELKRRQSGNRVEVIAYADKANE
                     RLRRRYRTLVLGKNKKQNVAKTAIARELSGFIWGMMTGRIA"
BASE COUNT         1376 a          757 c         1038 g         1548 t
ORIGIN      
        1 tttatcattc catttcagat gaggatgagg aacatgttga tgaactatat aagaaaatgt
       61 atcgtaacct agagtacgcg accatttcat tcaatgttgc tgtatcattg acattgttaa
      121 atttggttct aggatttggt gttaccttct tcgaagacgg ggcgatcatg tacgggtcaa
      181 tttttgatgt agttttctat gtcattcttc tcatttctca gatttttatc atgaaattaa
      241 ctcagaaaat tcgtgactat aagttatccg cttttgctac ggtaaaagaa atgaaggatt
      301 ttgctgaggc gatggacgaa ggtgagaaac aagcaaacta cgaaatgagt ttccaaatcg
      361 tcttcacttt gaatcaaatc gtgttaccag gtttgtatct attcctattt gtacttagca
      421 tgctattgca ggaacgacaa attacggcat ttctagttgt ggcgtttttg cacatttata
      481 tcaatgtgat gcaggttcgc atgatccgtc ggtattttaa ataaggaaag gttttggtag
      541 tgattatgaa agagaataaa aagtttatat cggttctaaa aattgtttac ttcgtggggt
      601 taatcggtct cggtctgtac gcagttggct atattttcgg agaatggctg gctactgggc
      661 agattgattt gtctaatctg aatatcctct tgccgatggt tttgggcttg ccagctctgc
      721 tattgttcaa aaaagaaagt gataagaatt gatttagtgt atgtgaagga gattgctatg
      781 aatagacttg gaaatgtcaa agattggaat gtggttaaaa attggaagtt tctcttagtg
      841 ggactggcag tcgttgcact gaatgtattg acccagttgg ccatgtttgc cctaccaaat
      901 tttgacggta ataatctact gattgctgca gttcttttat tgttagccgt ggtgcttggt
      961 cttatcttga ctggtaaatt aggtctatgg aaaggtgagc agaaatgggg gatggttgcg
     1021 aatgttgggt ttgtagtcct cgcttttatc gttatgtttg gtctgaaaat aattggtggg
     1081 caattgatta tgttggaaga gggatatggt caaacgactg ccaatcagga aatcatcaac
     1141 aattcaggct taccaacctt agttttgttt ctctttgcag ttttctttgc tccagttttg
     1201 gaggaactgc ttttcagagg gattcttatg gggagagtat ttggtaagga ttcgattgtg
     1261 ggattattgc tatcaagttt tttgtttgga cttattcaca atccgaccaa tatcggatct
     1321 tgggttgtct acggaggtat gggcctggtc ctgggactcg cctatcgtat ttctggcaaa
     1381 tataccaatg cacttattct acacagtctg aataatcttc ttggattttt gttgatgcta
     1441 ctgatgcaga gtctgggctt gatataggag gatacgatga aactattttt atggattcta
     1501 gtaggatttc tatttttcaa ttctctatca gataggaaaa aagtgaagca gttgcaagct
     1561 agggtaaaaa agttgcagaa aactacaaaa ggagaaaatc agatgtccgt tttattgaaa
     1621 gaattgatcg gaagtaaagc caaaatccgc tttgatgagg aattttctat tgcttatgaa
     1681 tacactattt tggcagttga tgaggaatgg gttaaaattt ctcgtgaatt agcgaatggg
     1741 gagattgaaa ccaagttggt acgggtcgat aatattattg acttgagctt ttagggaggg
     1801 attatgttaa aatttatcaa acacgtcgca ttactatttc tatactttgt tgcctatcaa
     1861 attgcttcag gttttctgat ggtaggacca actttacaaa gcataccaga catacctgcc
     1921 cagttgattg acagtacaat ttggatttgt gcgataattg gtcttgttct ctccattgcg
     1981 ctcattattc tgttatggaa gtatatctat ccacgtcatt ctgttgccta tcgtgtgaca
     2041 gcttcatggt tccataagat tcaatggccg atattgctct atattgcatt ttttatcatc
     2101 caatttatag taccagtccc agaaagtgaa aatcaaaagc ttgtgatcga attcgtatca
     2161 gcttatccct tgattgcatt tagctcagta gtgatctttg ctccaatttt agaagaattg
     2221 attttccgtg gattttttgc gacctatttc ttccctaaaa tggcagatat gaaggctgtt
     2281 ggaatctatc tatttgtaac gggggcttta tttagtctgg tccatatgcc agcaactctt
     2341 ccccaatttt tgatttattt cacgatggga ctcaatcttg gttggctcta tttaattaga
     2401 cgagatattc gttatccgat tgcgctacat atgttaaata atggaatttc ctatttgatg
     2461 attctatttt tggtatagac aatatgtaaa gacccccagt tgagaattcg tggatttacc
     2521 tgtacactag aataaaaaac aatcaacttc taacaggaat taccacactc aattggaggt
     2581 cttatatgtt tcattttacc acacttttca tcggaatgga tgttcacaaa gaaagttttt
     2641 cactctgcta ttatgatatg atggcgaatc aattcaaaca tagcactaaa gttggtccaa
     2701 atgttagcta tattgtgaac tatgtgaatg agcttcgtcg tttatatggt caagatgtag
     2761 aagtgttatg tggctacgaa gccggatgtc ttggatttac cctatatcac cagctacaag
     2821 ctcacgggat tccctgtatc gtgatggcgc ctacaacggt gatgaaggaa ggatctaagc
     2881 gtgttaagac tgataaaaaa gatgcagctc agctcacaaa agctctggcc tttcatagct
     2941 atcggcctgt tcatatttct actgttgagg atgaacaagt caaagaatat atctgcatga
     3001 gaacagacca caaagtggct ctgaagaaaa tcaaacaaca aattcttgcc ttctgtctcc
     3061 gacatgattt tcgctatacc gagggaagca gtaattggac acagaaacat gtccgctggc
     3121 tctgtcccct aaatattgat ggactttacg cagagatttt gacagaatat ctattgacct
     3181 atgagaaatt agtagatcaa atagaacggt atgatgcgcg aattgagcaa ctgggtcaaa
     3241 gcgacagtta tcaagagaag gtctctcagc ttttttgctt tattggcatt aaaacactaa
     3301 ccgctctttc cattgtgacg gaaatcggtg gttttaaccg ctttgcgaca gctcaacatt
     3361 ttgattttgc ttcttatctt gggctaactc ctagcgaaaa ttctagcggc gacagggaga
     3421 gaagaggtgc tatcaccaaa actgggaata gccatgtgag acgacttctg atagaagctg
     3481 cacaatcatt ggctaagggg acgattgggt ataaatccaa agaattgaaa aggagacaaa
     3541 gtggaaaccg agtggaggtg attgcttatg cggataaggc taatgaacgc ttaagaagac
     3601 gttatcgcac acttgttcta ggaaaaaata agaaacaaaa tgttgctaaa acagctattg
     3661 cacgggaatt atctggtttt atttggggga tgatgacagg aagaatagct tgaagttagc
     3721 acttgttttc atgtacactt ttgacaatta agttttatca tcgcagagcg atgaggaatc
     3781 cctatttcta tcccaaatca ctgggtgaat ttggacagaa ataaggattg aaataatgtt
     3841 tgaatggaga gtatcttgaa ggagtttagg acttcaaggt ttgagtcatc tacgaaacaa
     3901 ctaagtgaca caattgtgat ccacgcttat agagagtagg actcgcggca gaaccattga
     3961 cctgtgggta accaatccac gaatattaga gtggccaatg cccgaagcta aaaactaggc
     4021 tctttctaga tacttttcat tttttatcag ttcgattgtt tttacttgac aaagggcttt
     4081 acatatcagt cttttagttt gcttttagta ctcgcttcaa aggttcgggg aacctttgaa
     4141 ggctggagat agggcgaagc aagttctcct caatagctac aggttgctag aacagcctaa
     4201 aagctgttct aggttggaaa taaggaaacg acgtttcctc gctcgtcgaa gtaatcaaag
     4261 ataaactgat atgactatat aaaatgaatt acagaatata tgattgagat agatggtacc
     4321 ttgttgcacg ttgcaatagg gtatttttta tttagagtag taaacacagg gtaaaaatgt
     4381 taatgtttga gttcctatta acatctcggc ggagtagttg agttttcgaa tattctggag
     4441 attgtttgag gtgggaaatt ggtagtgagg gagtcagtcg tatcacttcc cgctcccatt
     4501 ttgtatggtt tactttcgtg aatgtagtat agccaatttt ggataatata tgaataatga
     4561 tatgtaaaat gtttactaat gattgataaa atcgaagaag aaaatcatac ttgtgttaag
     4621 tatattttat tgcttgggga atgaagatac tcgaatttta taagattata tcaagattcg
     4681 ttttgaaagt atttaaaata aatgataaaa accgttgac
//
LOCUS       DBNFCK010000122         4540 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.122, whole genome shotgun sequence.
ACCESSION   DBNFCK010000122 DBNFCK010000000
VERSION     DBNFCK010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.122"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            220..2907
                     /locus_tag="AC1PIW_001684"
     CDS             220..2907
                     /locus_tag="AC1PIW_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIP6606584.1"
                     /translation="MSKEQKRDLFYTQSEEQVLSSMGSSLDGLSSDEASKRLADYGRN
                     ELDEGEKRTLLAKFLDQFKDLMIIILIAAAILTVITEGSHGLTDAIIILAVVILNAAF
                     GVYQEGQAEAAIEALKNMSSPVARALRDNHVVEVDSKDLVPGDIVLLEAGDVVPADMR
                     LLEANSLKIEEAALTGESVPVDKDLSAVLAEDAPIGDRVNMAYQNSNVTYGRGLGVVT
                     NTGMYTEVGHIAGMLANADETDTPLKQNLHQLSKVLTYAVLVIAAVTMAVSVFVRGEG
                     ILPALMTSVALAVAAIPEGLPAIVTVVLSLGTQVLAKRNSIIRKLPAVETLGSTEIIA
                     SDKTGTLTMNQMTVEKVYTNGQLVDAKEALDASNTTLRVMNFANDTKVDPTGKLIGDP
                     TETALVQFGLDQNFDVREVLVSEPRVAELPFDSTRKLMSTVHQQAAGNFFVAVKGAPD
                     QLLKRVTQIEENGTIRPITDADKQAILATNKDLAKQALRVLMMAYKYVDAIPELESDV
                     VENDLVFSGLVGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGIIDP
                     NDTEDHVFTGAELNELTDEEFQKVFKQYSVYARVSPEHKVRIVKAWQNDGKVVAMTGD
                     GVNDAPSLKTADIGIGMGITGTEVSKGASDMVLADDNFATIIVAVEEGRKVFSNIQKT
                     IQYLLSANTAEVLCIFLATLFGWDVLEPVHLLWINLVTDTLPAIALGVEPAEPGVMQH
                     KPRGRNSSFFSGGVLSSIIYQGILQTALVLGVYGFALLYPEHAGDNHAIHADALTMAY
                     VTLGLIQLVHAFNVKSVYQSIFTVGPFKNKLFNWSIVAAFLLLMATVVIPGFNTFFKV
                     SILTPTQWMVTVIGSGLMVVVVEIVKFVQRKLGLDEKAI"
     gene            complement(3023..3214)
                     /locus_tag="AC1PIW_001685"
     CDS             complement(3023..3214)
                     /locus_tag="AC1PIW_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606585.1"
                     /translation="MTMESSKYFTPYYFSLAIVPIALLFNLPTLVKTFLLILALAITL
                     LGQAIFPKYIISTEKSSDH"
     gene            complement(3316..3516)
                     /locus_tag="AC1PIW_001686"
     CDS             complement(3316..3516)
                     /locus_tag="AC1PIW_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606586.1"
                     /translation="MRVIIHYPKPIKQLSSQKLPLLVDGKIAGTVTQGKILSLPITQP
                     SVTLSIRGDKKSSIQVKEGDRV"
     gene            3650..4066
                     /locus_tag="AC1PIW_001687"
     CDS             3650..4066
                     /locus_tag="AC1PIW_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001540 - amyloid-beta binding [Evidence
                     IEA];
                     GO_function: GO:0004930 - G protein-coupled receptor
                     activity [Evidence IEA];
                     GO_process: GO:0097190 - apoptotic signaling pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HIP6606587.1"
                     /translation="MNYLQNYILSKSSYLPSDKLFILQKELEDLDDEALNVLMMVEMR
                     QPLVALILAIFFGEFGVDRFYVGNKELGFAKLIAFAVSFVTLFILIGFLLFLGLYLWK
                     FIDCFLIMRACKEANFERLMLQIHQYKAFQHSNQTF"
     gene            4236..>4540
                     /locus_tag="AC1PIW_001688"
     CDS             4236..>4540
                     /locus_tag="AC1PIW_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIP6606588.1"
                     /translation="MQTKKMSMFLFFAYLLLLTWMIIFKMDLSIVYGRYGYASINLIP
                     FAGTAVYDGVLDFPEILFNIVSFIPFGIYMEMLFRKASWVTNLCLIMLVSLCFEVLQ"
BASE COUNT         1229 a          828 c         1020 g         1463 t
ORIGIN      
        1 gtggaaataa tctcttattt ccagaaaaat attcttgaat ttctcgggat ttttgatata
       61 atagttaaca aatcgaggga gtagctagtg gaaaattccg tgatattgtc gtcaatccga
      121 cttgtttccg gcaatatatt aaatagcgag acttgtttta ataaaacagg tctctttttt
      181 atttgttagg taaaaaaggg accgagaagg agaaggaaat tgtcaaaaga acaaaaacgc
      241 gatttgtttt atacacagag cgaagagcaa gtattgtcaa gcatggggtc ttctttggac
      301 ggtttgtcta gtgatgaggc aagcaaacgt ctagcagact acggtcgtaa cgaattggat
      361 gagggtgaaa aacgcacact cttagctaaa ttcttggatc agttcaaaga cttgatgatt
      421 atcattttga ttgcagcagc aatcttgacg gtgattacag aaggttcaca cggtttgaca
      481 gatgccatta tcatcttggc cgtggttatc ttgaacgctg cctttggtgt ttatcaagag
      541 ggtcaagctg aggcagccat tgaggcgcta aaaaacatgt caagcccagt tgcgcgtgcg
      601 cttcgtgaca atcatgtagt cgaagttgat tctaaagact tggtaccagg tgacatcgtc
      661 ttgcttgaag caggtgatgt tgtccctgcc gatatgcgtt tgttggaagc taactccctc
      721 aaaatcgaag aggcagctct tacaggtgag tctgtaccag ttgataaaga tttgtccgca
      781 gttttggctg aagatgcacc aatcggtgac cgtgtcaaca tggcctacca aaactctaac
      841 gttacttacg gccgtggtct tggtgtggtt acaaatacag gtatgtacac agaggttggg
      901 cacatcgctg gcatgcttgc taatgcagat gagactgata caccattgaa gcaaaacttg
      961 caccagctat caaaagtctt gacatatgca gttcttgtaa ttgcagcggt aacgatggct
     1021 gtttcagtct tcgtacgtgg tgaaggtatc ttgcctgctc ttatgacttc tgtagctctt
     1081 gcggttgcgg ccattccaga aggtttgcca gctattgtaa cagttgttct ttcacttggt
     1141 acacaggtct tggctaagcg taattccatc atccgtaagt tgcctgctgt tgaaacactt
     1201 ggttcaactg aaatcatcgc atcagataag actggtactt tgactatgaa ccagatgact
     1261 gttgaaaaag tctatacaaa cggtcaattg gtggatgcta aagaagcttt ggatgcaagc
     1321 aatacaacgc ttcgtgttat gaactttgcc aacgatacaa aagttgaccc aactggtaaa
     1381 ttgattggtg acccaactga aacggcactt gttcaatttg gtttggatca aaactttgac
     1441 gttcgtgaag tcttggtatc ggagcctcgt gtggctgaat tgccatttga ctcaacacgt
     1501 aaattgatgt caactgttca ccaacaagct gctggaaact tctttgtagc tgttaaaggt
     1561 gctccagacc aattgctcaa acgtgtaacg caaattgaag aaaacggtac aattcgtccg
     1621 attaccgatg cggataaaca agccattctt gccactaaca aggacttggc taagcaagca
     1681 cttcgtgtct tgatgatggc ttacaagtat gttgatgcta ttcctgaatt ggaatctgac
     1741 gttgttgaaa acgaccttgt cttctcaggt ttggttggta tgattgaccc tgagcgtcct
     1801 gaagcggcag aagctgttcg tgtcgctaag gaagcgggca tccgtccaat catgattacg
     1861 ggtgaccacc aagatacagc tgaggcgatt gcaaagcgtc ttggtatcat cgatccaaat
     1921 gatacagaag accatgtatt tacaggtgca gagctcaacg aattgacgga tgaagagttc
     1981 cagaaagtct tcaaacaata ctcagtatac gctcgtgtat ctccagaaca taaggttcgt
     2041 atcgttaagg cttggcaaaa tgatggtaag gttgttgcca tgactggtga cggtgttaac
     2101 gatgcaccat cacttaagac agccgacatc ggtatcggta tgggtatcac aggtacggaa
     2161 gtttcgaaag gtgcctctga tatggtcctt gcagatgaca actttgcaac tatcattgtg
     2221 gcggttgaag aaggacgtaa agtcttctca aacatccaaa aaaccatcca gtatctcttg
     2281 tcagctaaca ctgctgaggt attgtgtatc ttccttgcta ccctctttgg ttgggacgta
     2341 ttggagccag ttcatcttct ttggatcaat ttggtaacag atacacttcc agcgattgcc
     2401 cttggtgttg aacctgctga accaggtgtt atgcagcaca aacctcgtgg tcgtaactca
     2461 agcttcttct caggtggtgt cttgagttcg attatctatc aaggtatcct tcaaacagca
     2521 cttgtcttgg gcgtatatgg ttttgcactt ctttacccag agcacgctgg agataatcat
     2581 gccattcatg cggatgctct tacgatggca tatgttactc ttggattgat tcagttggtg
     2641 catgccttca acgttaagtc tgtttaccaa tctatcttta ccgttggtcc attcaagaac
     2701 aaactcttta actggtctat cgtagctgcc ttcttgctct tgatggcgac tgttgtgatt
     2761 ccaggattca ataccttctt caaggtatct atcttgacac caactcagtg gatggtaaca
     2821 gtaatcggtt caggtctcat ggttgtcgtt gtagaaattg ttaaatttgt tcaacgtaag
     2881 ttgggattgg atgaaaaagc tatctaatat agttattcag cttatctttt attacttcgt
     2941 taactcgact tgccgtactt cagtacagcc tgcgcctcgt taccttgtac taaaagcaaa
     3001 tatcatttga aaaagctcag atttagtggt ctgagctttt ttctgtactg ataatgtatt
     3061 ttgggaagat tgcttgtccc aataaagtaa tggctagtgc tagtatgagg aggaaggttt
     3121 tgacaagagt tggtaagtta aacagtaagg cgattggtac gatagccaag ctgaaatagt
     3181 agggagtgaa gtatttgcta ctttccatag tcatctgaat tagctttgat acatcgtcac
     3241 tttcgccttg ccgtacttca gtacagcctg cgcctcagtt ccttgtctga aagctaattc
     3301 attcgactat attatttaga ccctatcgcc ctccttaact tgaatgctcg attttttatc
     3361 ccctcgaatg cttagtgtaa cagatggttg tgtaattggt aagcttagaa ttttaccttg
     3421 agtaactgtc ccagcaattt ttccatctac caggagcgga agtttttggg aggatagttg
     3481 tttgataggt tttggataat gaataataac tctcatatga acctccttgt aatctagttt
     3541 cattatagca aaaaagaaaa aaatgtaaac gatttctgac actgttttcc cctacaggct
     3601 tgcttgaaga ataagaaact gctataattt tgaagaggta ggagtttata tgaattatct
     3661 tcagaattat attttaagta aaagcagtta tttgccatct gataagttat tcattctgca
     3721 aaaagaacta gaagatttag atgatgaggc tttaaacgtt cttatgatgg ttgagatgcg
     3781 tcaacctctt gtcgctctta ttttggctat tttcttcggt gagtttggtg tcgaccgttt
     3841 ttatgtcggg aataaagaat tgggatttgc caagctgatt gcttttgcag tatcttttgt
     3901 tacgctattt atcttgattg ggttcttgtt atttttggga ctttatttgt ggaagtttat
     3961 tgattgcttc ttgatcatgc gagcatgtaa ggaagcgaac tttgagcgtt tgatgctaca
     4021 aatccatcaa tataaggctt ttcagcatag caatcagaca ttttaagtaa gatatttttg
     4081 ggaataaggg gaggcttggt agtgtcttcc ttttctgttt ttagtaggct gtaaagtact
     4141 tgtaatatgg aacgagcttt gtcacagaag tgacatttta tagaacagca tttatgatat
     4201 aatgataaaa tatgctagtt gagaggagaa tttctatgca gacaaagaaa atgagtatgt
     4261 ttttattttt tgcctatctc cttttactta cttggatgat tatctttaag atggacttga
     4321 gcatcgtata tggacgctat ggttatgcta gtataaactt aattccgttt gcaggaacag
     4381 cggtctatga tggagtgctg gattttccag aaattctatt taatattgta agttttattc
     4441 cctttggtat ttatatggaa atgctatttc gcaaggcatc gtgggtgact aatctatgtt
     4501 tgattatgtt agtgagtctc tgctttgagg ttctgcaata
//
LOCUS       DBNFCK010000123         4527 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.123, whole genome shotgun sequence.
ACCESSION   DBNFCK010000123 DBNFCK010000000
VERSION     DBNFCK010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.123"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            53..877
                     /locus_tag="AC1PIW_001689"
                     /pseudo
     CDS             53..877
                     /locus_tag="AC1PIW_001689"
                     /inference="COORDINATES: protein motif:HMM:NF017927.6"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
     gene            1070..1906
                     /locus_tag="AC1PIW_001690"
     CDS             1070..1906
                     /locus_tag="AC1PIW_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HIP6606589.1"
                     /translation="MMTSDIVSRAMMTRERTRGTTLLFFPSILLSIVYAIPATIFRLL
                     TFLELRQNSAFVEGRLVSSIRLTYLGELGFALLLQLFIGLSCFHLLELLRGDRQEFSL
                     KDSFSFLKTEWKKPICTTFLYYYLLLFIAGLPANLGATLFIKGFTMSSLMVLSPGAMA
                     ELAVHQPSQLMKIGAPIFLLGMIIYLFVYYTYSQVVFVLYDHLDNASYTGPLAVFRES
                     RFLMKGNYWQRIWLDLSFLGWFIGELLTLHLLSFFVTPYYHLSRTIFYEKLKKQRLSA
                     VE"
     gene            complement(1924..2799)
                     /locus_tag="AC1PIW_001691"
     CDS             complement(1924..2799)
                     /locus_tag="AC1PIW_001691"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIP6606590.1"
                     /translation="MKTEFINEKAQSAFNLNIVTSPIVAIILLLVGDSLGAVIFTPIS
                     FLLPFSETLSVSFELFAFAFISLMVILWARYIEKSPWVGLGFTKKGAGKDFLLGWGIG
                     AAMLTTCTLIMWGLGAIEFTSIQFSTKLVGEFIILILAWSIQGTTEEILTRGWMFSSL
                     SARHNIPIGIIVSSLFFTFLHLGNNAISLIPILDLTLFAVLACLIMLKTGNIWVIGGL
                     HAAWNCFQGNVFAFSVSGTDAGQAFIQIGITGPEWLSGGKFGVEGSVISLLVQGIMIC
                     WLVYDLYFKPKTSSL"
     gene            2922..3533
                     /locus_tag="AC1PIW_001692"
     CDS             2922..3533
                     /locus_tag="AC1PIW_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3885 domain-containing protein"
                     /protein_id="HIP6606591.1"
                     /translation="MELSQWLKSFQLKDNRLVGPFFYGTPLALRFEIGPADEAEELSR
                     AIYLERAYARAVELFEQASSEYDYVLLSLLRQEDRDIDTYLWHFSSKFNFNKVPESEL
                     IEVEDWTGDVLVFERYLFPVTDQDLNALLREIVKADHGGFNYLSSSVLFLSSQDNIIY
                     HCYDNRGVDIAVLADDKRLELFTDCHDLLLDYDMEEMERRVRG"
     gene            4174..4266
                     /locus_tag="AC1PIW_001693"
     CDS             4174..4266
                     /locus_tag="AC1PIW_001693"
                     /inference="COORDINATES: protein motif:HMM:NF028245.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HIP6606592.1"
                     /translation="MSVAEAFGLMFQFSTLLIVLLTFIFNNRKK"
     gene            complement(4281..>4527)
                     /locus_tag="AC1PIW_001694"
     CDS             complement(4281..>4527)
                     /locus_tag="AC1PIW_001694"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606593.1"
                     /translation="GILLFLGCQAVFDDVDKKLSNDFGLWYNSFGKGIERQLNPSIVF
                     FRFRETTSNSLPDCLNILKTGQMKITVNLLVHSVKVS"
BASE COUNT         1321 a          813 c          962 g         1431 t
ORIGIN      
        1 taatagcgat attcgagcta aagcaacggt tattcgtgaa caaacagata ggatgataac
       61 cttgtttttg gcacctgttt tgatttcttt tctgtcagga gtggcagaat ttggcttgaa
      121 acaaatctgg gggcaaactg gtactttctc ctggggcaca actattgtga aagacggtgt
      181 tacttggact catcatttta tttctgttgg cccgtcaatc atatttgatt ttattgcaca
      241 atgtttaatt gtaacagctt gttttcaact aataagactg gtgagaaagc aaagaaccaa
      301 ggttagcttt acggagtgtt tcactttact agatggggag aattttttac cagtttttag
      361 tacgacatta ttgaaaatgc tggtcttgta tttagcaagt ttccctgctg tttttggaat
      421 gatattgata ggtttagctt tctatagtac tattgtcatc tttggtgctc ctattgatta
      481 tcagcctgat ttttcagctt ttttcagttc gtcttatttt caaacaggat taatactggt
      541 atgtgtaggg attatttgta gtttattcgt aggatatggg ctcagtcagg tcacttttct
      601 tctctatgat tatttggaaa acaataccta ttcttcacca tttaaactct tcagttagag
      661 ctggcagttg atgaagggga ataaatggcg ccgtttcctc ttggatttat cttttgttgg
      721 ctggtttatt ggagttcttt tgacgtttgg gtttttggga ctttacgtat atccatacta
      781 ttggacttgt cagactatat tttatgaaga tttgaaggcc aagaatccat tggtgttttc
      841 aacatataaa tcagttatct cttccatagt agcctaggga gtatggagat aacaaattat
      901 taggaatccc ttcgtatttt ggagggattt tcctgttttt aagagatgtt ctggaaaatt
      961 aatggataaa gttttatact aatagtagga tggtaaagta gtccgatgta gatataatat
     1021 ttgcgacgtg actttataaa taaggagaga aaattggagg ggaattgtga tgatgacgag
     1081 tgatatagta tcaagagcga tgatgactcg cgagcggaca agaggtacaa cactactatt
     1141 ttttccctcc attttgctat ctattgttta tgctatacca gcaacaattt ttcgcctact
     1201 aacatttcta gagctccgtc aaaacagtgc ttttgtggag gggcgattgg tatcttcaat
     1261 caggttgacc tatttaggtg agttaggctt cgctctgttg ttacagctgt tcatcggtct
     1321 gtcctgtttt catttattgg aattgttgag aggggataga caggagtttt ctttaaaaga
     1381 tagtttttca tttttaaaga cagaatggaa gaagcctatc tgtaccactt ttctttatta
     1441 ttatttgctt ctttttatag ctggacttcc agctaatcta ggggctactt tatttattaa
     1501 aggcttcaca atgagtagtt tgatggtctt atctccgggg gcaatggcag agctggcagt
     1561 tcatcagcct agtcaattaa tgaagatagg ggctccgatt ttcctgctag gaatgattat
     1621 ttaccttttt gtctactata cctactcaca ggttgttttc gtcttgtatg accatttgga
     1681 taatgctagt tatactggtc ccctggctgt atttagggaa agtcgtttct tgatgaaagg
     1741 gaactattgg caacgtattt ggttagattt atccttttta ggttggttta ttggggaatt
     1801 attgaccctt catctgttat ctttttttgt gactccttac tatcatttat ccaggactat
     1861 tttctacgaa aaattgaaaa aacaaagact gtcagctgta gagtagctaa cagtctttta
     1921 tgattatagg gagcttgttt tgggcttgaa atagagatcg taaaccaacc agcaaatcat
     1981 tataccttgg acaagcaagc tgatgacgga gccctctacg ccaaactttc caccagatag
     2041 ccactcagga ccagtgatac cgatttgaat aaaggcctga ccagcatcgg tgccgctgac
     2101 tgagaaagca aaaacattcc cttggaaaca gttccacgca gcgtgtaagc caccaatcac
     2161 ccaaatgttg cctgttttga gcataatgag gcaggcaaga acggcaaata gagttaaatc
     2221 caaaattgga atgagtgaaa tagcattgtt tccgaggtgg agaaatgtga agaagaggga
     2281 agaaacaatg ataccgatag gaatattatg tctagcagaa agtgatgaga acatccatcc
     2341 tcttgttaga atttcttctg tcgtaccttg gatagaccag gcaaggataa ggataataaa
     2401 ttctccgact aattttgtag agaactggat gcttgtaaat tcaatcgcgc ctaatcccca
     2461 catgatgagt gtacaagtgg tcagcattgc agctccgatt ccccacccga gtagaaaatc
     2521 ttttcctgca ccttttttag taaaaccaag tcctacccac gggctttttt caatataccg
     2581 tgcccataaa atgaccatca ggctgataaa agcgaaagcg aacagttcaa aacttacaga
     2641 aagggtctcg ctaaatggga gtaaaaagct tattggagtg aaaataacag ctcctagtga
     2701 atctcctact agcaacaaaa taatagctac aataggagag gtgacgatgt tgagattgaa
     2761 agctgattga gccttctcat tgataaattc ggttttcata ggacctcctt ttactagata
     2821 ctttactagt tcactattct atcatatttt tcaaaagaag actagaccta atttcctagt
     2881 atatggtaga atagtacaaa gctatataag gagaatagta tatggaattg agtcaatggc
     2941 tgaagtcctt tcaactgaaa gataatcgac tagtgggccc attcttttat ggcacacctc
     3001 ttgctcttag atttgaaatc gggcctgcag acgaagcaga agaattatca agagcaatct
     3061 atcttgaaag agcctatgca cgtgcagtag aactttttga gcaggctagt tcagaatacg
     3121 actatgtgct cctttcgctc cttcgacagg aagataggga tatagacacc tacctctggc
     3181 atttttcatc gaaatttaac ttcaacaagg tccctgagtc tgagttgata gaagtggagg
     3241 attggacggg agatgtgcta gtctttgagc gctatctctt tccagttacc gaccaggacc
     3301 taaacgcttt gttgagggag attgtcaaag ccgaccatgg aggttttaac tatctatcaa
     3361 gttccgttct cttcctatct agtcaggaca atatcatcta tcattgctac gataatcggg
     3421 gagttgatat tgctgtttta gctgatgaca aacgtttgga gctctttaca gactgccatg
     3481 acctcctttt ggactatgat atggaggaga tggagagaag ggtgaggggg taggaaacca
     3541 ttactaaagg tgatatacca tctcttagat aaaagtactt cacaagtgaa tacaggttca
     3601 aaaaaatcca cagtaaaacc tgtggaaaat atggtagaat agaacagtag aaaagttcca
     3661 aaagtggagc ggaaatctat tttgggaggt ggcaacttca tttttgatgg aaagtaaaac
     3721 tttctcatca aaaaacgagg ctgggcaaaa agtccaaagc cacttctcag agttcgtgtc
     3781 aacatctcag cgcagtggtt gtttgggttt aacagtccag tggactgtta aaggttggag
     3841 ataggatttg caaagcaaat cacagcagtt cgtgtttcac actccaaatc tgaccattac
     3901 gactgatgcg aacagagttc gcttcatctc caacctcaaa ctgtctccca gacagtttga
     3961 gctgtgcggg gggtgggagt gaaacagtct gggaatagac tgtttcagct caacaactta
     4021 aaacgaaaaa ttgttgacga actctttttg acaagttgag ttctttccca ctccctaaaa
     4081 gttgcactaa ttcgattttt gaaaaatatt gtttcaaaaa tctcatgtcg atgccagaga
     4141 caatcagcat ctctattgat tttactctaa ctaatgtctg tcgcagaagc cttcgggctg
     4201 atgtttcagt ttagcacgtt gctaatcgtg ctactgacat ttatttttaa caatcgtaaa
     4261 aaataaaaga cagccaaccg ctacgaaact ttgaccgagt gaactagcag gttgactgta
     4321 atcttcattt gtcccgtctt taaaatgttc aaacagtctg ggagactgtt tgaggtcgtt
     4381 tctcgaaaac ggaagaaaac gatactggga ttgagttggc gctcaatccc ttttccaaaa
     4441 ctattatacc acaaaccaaa atcgtttgac aattttttat ctacatcatc aaaaacggct
     4501 tgacagccta agaaaaggag aatacca
//
LOCUS       DBNFCK010000124         4499 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.124, whole genome shotgun sequence.
ACCESSION   DBNFCK010000124 DBNFCK010000000
VERSION     DBNFCK010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.124"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..677
                     /locus_tag="AC1PIW_001695"
     CDS             <1..677
                     /locus_tag="AC1PIW_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0065002 - intracellular protein
                     transmembrane transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIP6606594.1"
                     /translation="LGESAIFVAALVVLIFFVVYFTTFVQQAEYKLPIQYTKRAQGAP
                     SSSYLPLKLNPAGVIPVIFAGSITAVPTSLIQYFASQNKSAGWLLTVQEYFDYSTAKG
                     MIVYAGLIIAFTFFYTFVQVNPEKTAESLQKSAAYIHGVRPGNGTEQFLSKLLTRLAV
                     IGALFLSFVALLPILAQNLFGLSSSIAFLGTSLIIVISTSIEGIKQLEGYLLKRKYVG
                     FLEITE"
     gene            771..1412
                     /locus_tag="AC1PIW_001696"
     CDS             771..1412
                     /locus_tag="AC1PIW_001696"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIP6606595.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEKFNVAHISTGDMFRAAMANQTE
                     MGILAKSYIDKGDLVPDEVTNGIVKERLVQDDIKEKGFLLDGYPRTIEQAHALDENLA
                     DLGIELQGVINIEIDPSKLVERLSGRIIHKETGETFHKVFNPPVGDYKEEDFYQREDD
                     KPESVKRRLEVNIAQGQPIIDHYRAKGLVHDIEGDQDIDLVFQAIDTVLSKLQ"
     gene            1534..1752
                     /gene="infA"
                     /locus_tag="AC1PIW_001697"
     CDS             1534..1752
                     /gene="infA"
                     /locus_tag="AC1PIW_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIP6606596.1"
                     /translation="MAKEDVIEIEGKVVDTMPNAMFTVELENGHQVLATVSGKIRKNY
                     IRILVGDRVTVELSPYDLTRGRITYRFK"
     gene            1777..1893
                     /gene="rpmJ"
                     /locus_tag="AC1PIW_001698"
     CDS             1777..1893
                     /gene="rpmJ"
                     /locus_tag="AC1PIW_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HIP6606597.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            1913..2278
                     /gene="rpsM"
                     /locus_tag="AC1PIW_001699"
     CDS             1913..2278
                     /gene="rpsM"
                     /locus_tag="AC1PIW_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HIP6606598.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VKDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            2296..2679
                     /gene="rpsK"
                     /locus_tag="AC1PIW_001700"
     CDS             2296..2679
                     /gene="rpsK"
                     /locus_tag="AC1PIW_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIP6606599.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKTVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            2724..3662
                     /locus_tag="AC1PIW_001701"
     CDS             2724..3662
                     /locus_tag="AC1PIW_001701"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIP6606600.1"
                     /translation="MIEFEKPTITKIDENKDYGRFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSIKIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKKIELDVVGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIADGATFKAVLTVNSGRGYVPAEDNKKDDAPVG
                     TLAVDSIYTPVKKVNYQVEPARVGSNDGFDKLTLEINTNGTIIPEDALGLSARILMEH
                     LGLFTDLTEVAKSAEVMKEAEVASDDRMLDRTIEELDLSVRSYNCLKRAGINTVFDLT
                     EKTEPEMMKVRNLGRKSLEEVKVKLADLGLGLKKDK"
     gene            3677..4063
                     /gene="rplQ"
                     /locus_tag="AC1PIW_001702"
     CDS             3677..4063
                     /gene="rplQ"
                     /locus_tag="AC1PIW_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HIP6606601.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSATALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
BASE COUNT         1418 a          816 c          948 g         1317 t
ORIGIN      
        1 gtttggggga atcagcaatc tttgttgctg cactcgtcgt attaatattc tttgttgtgt
       61 attttacaac ttttgtacaa caagcagaat ataaattacc aattcaatat acaaaacgtg
      121 cacaaggagc accttctagc tcatatttac ccctgaaatt gaatccagca ggagttattc
      181 ccgtaatctt tgcaggctca atcactgcag ttccaacttc tttgattcaa tatttcgcaa
      241 gtcaaaataa gagtgccggg tggttattga cggttcagga atactttgat tattcaactg
      301 ctaaaggtat gattgtatat gcaggtttaa ttattgcctt tacattcttc tacacctttg
      361 ttcaagtaaa tcctgagaag actgcagaaa gccttcagaa aagtgcagcc tatatccatg
      421 gagttcgtcc tggtaatggc actgaacagt ttttgtcaaa attattgaca agattggcag
      481 taatcggtgc actcttcttg agttttgtag ctttgctgcc tatccttgcg caaaatctct
      541 ttgggctttc ttcaagcatt gcgttccttg gtacaagttt gattatcgta atctctacaa
      601 gtatcgaagg catcaagcaa ttagaaggct accttcttaa gagaaaatat gtaggtttct
      661 tagaaattac agaatagaat ataggttgac ctagtcaacc ttctattttg tttttagata
      721 agtttgtcaa taatggcaaa tttatgtgaa aacaaaataa ggagtttgtc atgaatcttt
      781 taattatggg tttaccaggt gctggtaaag ggacacaagc ggccaagatt gttgagaaat
      841 tcaatgttgc tcatatctca acaggagata tgttccgtgc agcaatggcc aatcagactg
      901 aaatgggtat tcttgccaag tcgtacattg ataagggtga cctcgttccc gatgaagtta
      961 ctaatggcat tgtcaaagaa cgtttggttc aggatgacat caaggaaaaa ggctttttgc
     1021 ttgatggcta tcctcgtacg attgaacaag cccatgcctt ggatgaaaat ttggctgact
     1081 taggaattga gttgcaaggt gttatcaata ttgagattga tccttcaaaa ttggtagagc
     1141 gtctcagcgg tcgtattatt cataaagaaa ctggagagac tttccacaaa gtattcaatc
     1201 cacctgttgg agactacaaa gaggaagact tctatcaacg tgaagatgac aagccagaat
     1261 ctgtcaaacg tcgtttagag gtcaatatcg cacaaggaca gccaatcatt gatcattatc
     1321 gtgcaaaagg gctggtccat gatattgaag gtgaccaaga cattgacctt gttttccaag
     1381 caattgatac agtactatca aaattgcaat aaactaatag atttggcttg cattttttat
     1441 acaaagatga taaaatagtc tagtctgact tataattgtt acctctgtgt tcagaggaca
     1501 tcaaatcgat atttagagag gggttacttt tgcatggcaa aagaagatgt gattgaaatt
     1561 gaaggcaaag tagtcgatac aatgccaaat gctatgttta ctgttgagtt ggagaatgga
     1621 caccaagtcc ttgcaactgt ttcaggtaaa atccgtaaaa actacattcg tattttggtc
     1681 ggtgaccgcg taactgttga gcttagtcca tacgacttga cacgtggacg tatcacatac
     1741 cgctttaaat agtcgaaata cttggaggga ttaaacatga aagtaagacc atcggtcaaa
     1801 ccaatttgcg aatactgcaa agttattcgt cgtaatggtc gtgttatggt gatttgccca
     1861 gcaaacccta aacacaagca acgtcaaggt taagaaatag aaaggagaaa acatggctcg
     1921 tattgctgga gttgacattc caaatgacaa acgtgtagtt atttcattaa cttatgttta
     1981 tggtatcggt cttgcaacat ctaaaaaaat tcttgcagct gcaggtattt cagaagatgt
     2041 acgcgtgaaa gatttgacat cagatcaaga agatgctatt cgtcgtgaag ttgatgcaat
     2101 caaagttgaa ggtgacctcc gtcgtgaagt taacttgaac atcaaacgtt tgatggaaat
     2161 cggttcatac cgtggtatcc gtcaccgtcg tggacttcct gtccgtggac aaaacactaa
     2221 aaacaatgcc cgcactcgta aaggtaaagc tgttgcgatc gcaggtaaga aaaaataaaa
     2281 taggaggtag aaaaattggc taaaccaaca cgtaaacgtc gtgtgaaaaa gaatatcgaa
     2341 tctggtattg cacatattca cgcaacattt aataacacta ttgttatgat tactgatgtg
     2401 catggtaacg ctattgcttg gtcatcagct ggtgctcttg gtttcaaagg ttctcgtaaa
     2461 tctacaccat tcgcggctca aatggcttca gaagctgctg ctaaatctgc acaagaacac
     2521 ggtcttaaaa cagttgaagt taccgttaaa ggcccaggtt caggtcgtga gtctgctatc
     2581 cgcgctcttg ctgccgctgg tcttgaagta acagcaattc gtgatgtgac tcctgtacca
     2641 cacaatggtg ctcgtcctcc aaaacgtcgc cgtgtataat catacattcc atacacagct
     2701 tttcgtttaa gagggagtaa gaaatgattg agtttgaaaa accaacaata acaaaaattg
     2761 atgaaaataa agattacggc agatttgtaa tcgaaccatt agaacgtggt tacggtacaa
     2821 ctcttggtaa ctctcttcgt cgtgtacttc ttgcctcact tccaggtgct gcagtaacat
     2881 cgattaaaat tgatggtgta ctccacgaat tcgacacagt tccaggtgtt cgtgaagatg
     2941 ttatgcaaat tattcttaac atcaaaggca ttgctgtaaa atcttatgtc gaagacgaaa
     3001 agaaaattga acttgatgta gtaggtccag ctgaagtaac agcaggagac attcttacag
     3061 atagtgacat tgaaattgta aaccctgacc attatctctt taccattgct gatggtgcca
     3121 cttttaaagc tgttttgact gtcaattcag gtcgtggtta tgtaccagct gaggataata
     3181 agaaagatga tgcaccagtg ggaacacttg ctgtagattc tatctatacg ccagtgaaga
     3241 aagtcaatta tcaagttgaa ccagctcgtg ttggtagcaa cgatggtttt gacaaattaa
     3301 cacttgaaat caacacaaat ggcaccatta ttccagaaga tgctttaggt ctttctgcac
     3361 gtattttgat ggagcactta ggtctcttca ctgacttgac tgaagttgca aaatctgcag
     3421 aagtgatgaa agaagctgaa gtggcttcag atgatcgtat gcttgatcgt acgattgaag
     3481 aattggatct atctgttcgc tcatataact gtctgaaacg tgcaggcatt aacactgtat
     3541 tcgacttgac agagaaaact gagccagaaa tgatgaaagt gcgcaatctt ggtcgcaaga
     3601 gtcttgaaga agttaaagtt aaattggctg atcttggtct aggattgaaa aaagataaat
     3661 aatataggag gaaatcatgg cataccgtaa actaggacgc actagctcac aacgtaaagc
     3721 aatgttgcgc gatttgacaa ctgacctttt gatcaacgaa tcaatcgtta caactgaagc
     3781 tcgtgctaaa gaaatccgta aaacagttga aaaaatgatc acacttggta aacgtggtga
     3841 tttgcacgca cgtcgtcaag cagcagcatt tgttcgtaac gaaattgcat ctgaaaacta
     3901 tgatgaagca actgataagt acacttctgc tacagcactt caaaaattgt tctctgaaat
     3961 tgcacctcgt tatgcagaac gtaatggtgg atatactcgt atcctcaaaa ctgaaccacg
     4021 tcgtggtgat gctgcaccaa tggcaattat cgaacttgta taagatcatc aattttgttg
     4081 agtgttatga tgatggaatc ctattctgat tcttagtcta gctctggtct accgctgagc
     4141 ttgtctcagc ggtaacactc atcatgttga ttggacgctt gtttacgaaa ttactctaag
     4201 atagaataat ttcgtaagca ggcgttttta ttttactcaa aaattttgat atactggagg
     4261 catacgaaga aaatatacaa tctccgatta gtatgcctct cagtttttcg ccaaatttca
     4321 taaaaaaatc caaaaaaccg gttgacaatt gccaaagtag atgatagaat aaatgagttg
     4381 tctcttgagg gactagttaa cagaggaacg aaaaaagttt cgaaaaagtg ttgacaagac
     4441 catcagaaga tgatagacta atagagttgt ctcttgaggg actggttaac agaggaacg
//
LOCUS       DBNFCK010000125         4349 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.125, whole genome shotgun sequence.
ACCESSION   DBNFCK010000125 DBNFCK010000000
VERSION     DBNFCK010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.125"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            30..215
                     /locus_tag="AC1PIW_001703"
     CDS             30..215
                     /locus_tag="AC1PIW_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606602.1"
                     /translation="MSQPVVPLTIPQSRRFEKKSRNDILMKIRLGKTEVTFFQSINPE
                     VMETILDKVLHYDHPTQ"
     gene            196..483
                     /gene="tnpB"
                     /locus_tag="AC1PIW_001704"
     CDS             196..483
                     /gene="tnpB"
                     /locus_tag="AC1PIW_001704"
                     /inference="COORDINATES: protein motif:HMM:NF017528.6"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HIP6606603.1"
                     /translation="MTIRLSDLGQVFLVCGKTDMRQGIDSLAYLVKSQFNLDPFSGQV
                     YLFCGGRKDRFKALYWDEQGFWLLYKRFENGKLAWSNNEEEVKALTSEQVD"
     gene            600..806
                     /locus_tag="AC1PIW_001705"
     CDS             600..806
                     /locus_tag="AC1PIW_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HIP6606604.1"
                     /translation="MDELLAIIKQQTSTIEQLTNELTLLREQVDYLRQKLYGKSSEKV
                     VYQPGQLSLFGEESLPEEEADLPS"
     gene            760..2092
                     /gene="tnpC"
                     /locus_tag="AC1PIW_001706"
                     /pseudo
     CDS             760..2092
                     /gene="tnpC"
                     /locus_tag="AC1PIW_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941577.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            2296..3117
                     /locus_tag="AC1PIW_001707"
                     /pseudo
     CDS             2296..3117
                     /locus_tag="AC1PIW_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(3477..4348)
                     /locus_tag="AC1PIW_001708"
                     /pseudo
     CDS             complement(3477..4348)
                     /locus_tag="AC1PIW_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730292.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1357 a          787 c          951 g         1254 t
ORIGIN      
        1 gttataaaaa acaaactgga ggattcatta tgtcacagcc cgtcgtccca ttgactattc
       61 cccaatctcg ccgctttgaa aagaaaagca gaaacgatat tctgatgaaa attcgtctcg
      121 gaaaaacgga agtcacattc ttccaatcta tcaaccctga agtaatggaa acaatcttgg
      181 ataaggtact gcattatgac catccgactc agtgatttag gtcaagtctt cttggtctgt
      241 gggaaaacgg atatgcgtca ggggattgac tctctcgcct atcttgtcaa aagtcaattt
      301 aatctggatc ccttttcagg tcaggtctat ctcttctgcg gaggtcgaaa agaccgattc
      361 aaagctcttt actgggatga acagggattt tggttattgt ataaacgttt tgaaaatggg
      421 aaattggcct ggtcaaacaa tgaagaagaa gtcaaggctc taacttccga acaagtagat
      481 tagctcatga aaggattttc tatcattcca aaaataaatg tttcaaaaag tcgtgatttc
      541 tattgaaatc atggcttttc tttgtgtata atgagataaa aagaaggagg agcgagcgta
      601 tggatgagtt gttggcaatt attaaacaac agacctcaac gattgaacaa cttaccaatg
      661 agctaaccct ccttcgagaa caggttgact atctgagaca gaaactctac ggaaaatcat
      721 cagagaaagt tgtgtatcaa cccggtcagc tgagcttgtt tggggaggaa agcctccctg
      781 aagaagaagc tgacttaccc agttgaaaca gagatcatca cttataaacg caagaaaact
      841 aagggagttc gtcaggctat ttttagtcag ttcactccag agatagtgca tcacgaattg
      901 cagggcgaag actgcacttg tccagactgt catggtcagt tgaaagagat tggctcagct
      961 gttcaacgac aagagttggt cttcattcct gcacaattaa agcggattga ccatgttcaa
     1021 cacgcataca agtatcaggc atgtagccag aagaatctga gcgataagat tatcaaggct
     1081 cccgttccta aggcaccttt ggcacacagc ttgggttcag cctctatcat cgctcacacc
     1141 attcatcaga agttcaattt gaaggtgcca aattaccgtc aggaagagga ttggcataag
     1201 cttggcctgc ccatcagtcg gaaggaaata gccaactggc atatcaagtc tagtcagtat
     1261 tatttcgagc cgatttacaa cctgttgcac gagaaattgt tggagcagcc tattctccat
     1321 gcggatgaga ccgcctacaa ggtcttagaa aatgatggtc agttgacctt cttgtctggg
     1381 aaacatgagg aaaatggaat taccctctat catcatgaca aacgacggag tggcttggtt
     1441 gtggaggagg ttcttgggga ctatgcgggc tacgtacatt gtgacatgtg gagtgcttat
     1501 cgtcaattag acaaggctca gctggttggt tgttgggctc ccgtcaaacg aaagttcttt
     1561 gaggcgactc ctaagaagac agacaagact tctttaggag tccaagggct tagcctattg
     1621 cgaccgcctg tttgccttgg agagttgact gggctgactt gtctagcgag gagcgactgc
     1681 ataaacgctg gctcccttga tggacgagtt ctttgactgg tgtcgtgagc aatctgtttt
     1741 gccaggctcc aaattgggta ctgctttgga gtatagcctc aaatacgaaa ccaccttccg
     1801 aaccgttctc tcggatggtg acttggtcct gtctaacaac atggctgaaa gggctatgaa
     1861 gaccttggtg atgggcagaa aaaattggtt gttctcccaa agttttgagg gagccaagtc
     1921 gacagctatc attttgagtc ttttggaaac ggccaagagg cacgaacttg atacagagaa
     1981 atatatgacc tatcttctag acaatctacc taacgaggaa acactcgcta aaaaagaagt
     2041 tctagaggct tatttgccat gggagaagaa aattaagaga gcatgtcaat agaaaaaggt
     2101 tccagagtca acaggacttt ggagcctttt caatatacct tattattggg cggttacaaa
     2161 gtagcctagg aaacgaagcc gaagatagaa ctggagttgg agttcatcaa ggcaagttga
     2221 caacggataa ttttgatttt cgaagagtat caataaaaat caaagatagc aagtctaagc
     2281 tacttatgaa agttctataa tgaagtcaaa cgtgggacaa ctttacaaca agtgcgaaaa
     2341 gggctgtaca aaaaagtcta ttctgctgac tatgcacaaa cggtataccg aactaatcga
     2401 aaacgatcgg taaaaaagat aatcctaacg aaagaattga aagagaaaat tttacactat
     2461 actaagcaaa aattttctcc tgaaatgatg gttaagaaga agaaaatgaa agtgggtatt
     2521 tcaaccattt actattggtt tcatcatggc catttaggat tgacgaaagc agatatgctt
     2581 taccctagaa aagggaaaag ggtcaagaag caagctagtc ctaactttaa accagcagga
     2641 aaatctatcg aagaacgtcc tgacgttatt aatcttcgct tggaaaacgg tcattatgaa
     2701 attgatacag ttctactaac cagagggaaa aatcattgcc tcttagtctt aaccgaccga
     2761 cgaagcaggc accaaatcat acgattaatt ccaaacaaaa ccgccgaatc ggtcaatcag
     2821 gcactggagg gaatcttgag ggaacatcgt atcctgtcta taactgtaga taatggtaca
     2881 gaatttagtc gtctggctga agtgtttcct gaggagtata tctactatgc gcatccctat
     2941 tcttcatggg agaggggaac gaatgaaaat cacaatcgat tgattcggag atggttacct
     3001 aaaggaacca agaaaacgac tccaaaagaa gtcgctttta tcgaacattg gatcaacaac
     3061 taccctaaaa aatgcttgga ctacaagtcg ccgagtgaat ttcttatggg tggctaattt
     3121 caacttgaaa tttggggtct aagctactta taacatttga aaccttaatt tataagtttg
     3181 agttgaggat tgtggatttc aagcagctgt tttcttctaa aaaaataaca aaatcgaaac
     3241 ggtccatatc cactttgagt ggtagttggc tatttttgat tttaaatgag tatcacaccc
     3301 catcgtccca ttgactgttc cccaatctcg ccgctttgag aagaaaggca gaaatgatat
     3361 tctgatgaaa attcgtctcg gaaaagtgga agtcacattc tttcactcta tcaaccaaga
     3421 aacattagaa acaatcttag ataaggtact attttatgac catccgactc agtgatttag
     3481 gtcaagtcta cttggtttgc gggaagtagt ggtgtgattt ggttaagagg attatgctca
     3541 cttagaattt ctacctgctt gctttgagag acatgagtgt agatttgtgt tacattgatg
     3601 ttactgtggc ccagtaaatg ttgtatttgt cgaatatcga cgccattatc aagcaacata
     3661 gttgcaaaac tatgtcgaaa catatgtgga gtgatatgtt ttttcagttg tagcgagtga
     3721 gaaatatttt tcagacttaa gcgcacactt tgttctttaa gatgatcatc gttttcacac
     3781 tggtaaatag ataactagaa tgactacaat gatgcgctat atagtcttca agtaacgaaa
     3841 gtgttttatc gtttccaata tagagtagtc gcactttctt tccttttccg agtatttgaa
     3901 tggttctatt ttccaaattg atattatcaa gttttaaatt acataattca gagatgcgta
     3961 gaccagttga taaaagtagc gagataagga gtagatttcg catagctttc cgattagaat
     4021 gcttattttt agaattttct atttgttggt gggcgaaaca atatattttt tgtaaatctt
     4081 tcaaggaaat cgttttagga agctttttct cttccttgta gtggagtcga agctgatgga
     4141 agggattttt tgaaattagt ttttcttctt ccagaaattt atagtatgat ttaagactag
     4201 caatcttacg ttttaatgtg ctagtctttt ttatatgttg gttgagaaaa gtaagatagg
     4261 acctaatatc attggtccta aagctataat actaatgtaa attggatttg tatgcttgga
     4321 tagtatgaga actaagtcgt ctttgaata
//
LOCUS       DBNFCK010000126         4202 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.126, whole genome shotgun sequence.
ACCESSION   DBNFCK010000126 DBNFCK010000000
VERSION     DBNFCK010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.126"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            87..809
                     /locus_tag="AC1PIW_001709"
     CDS             87..809
                     /locus_tag="AC1PIW_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606605.1"
                     /translation="MEKEVFTPDQSQSIVDAILKGYKSYLDERIEKRRTMHVSAGYVW
                     TKANHIDGALATANLNFIDKFELKHASQSWEFLEFNSENQLLGRVCFVLKGLTRLRQV
                     FPTAVKSGKGYLFDYAQINTSYLENLSRHTDESTLFPVQMELFDDNLFELLNRSEQSI
                     NNFFFITYQADELHHLTNIQVIMPDSRTGKLIEIQNLSTFISTSNVDFNDEKYQELQG
                     LSEIDDVADFEIIPRLASEKEA"
     gene            822..1976
                     /locus_tag="AC1PIW_001710"
     CDS             822..1976
                     /locus_tag="AC1PIW_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HIP6606606.1"
                     /translation="MFNGSRLEELRLLNGLTRADLARELEVTEQAIWQFETGVTLPKM
                     KNKIAMARFFQVEVDYFDAVDKNGSFSLSRIAFRNADLEAKKNIHIQMVYLEKMNQMI
                     DYLENFVSIPNQIIYQLVDSIEQKLQQHESLEFIADHVREVLAVSVDNANLLYSLEKS
                     GIYISERLINGNADAYSAWSRNGRPFIVLGLGKSAVRHNFDLAHELGHLLLHNQVDFE
                     ELSKEELEEKEKEANQFASYFLLPRKQFLMDFVNIVGKRVSNPDQYILLKKRFGVSIQ
                     ALEYRAYKLGLLTPQQNSYFYRQISKKGYKAVEPLDLEIPLKKPSKIRSILDVILSNN
                     LLTLSDIERNQRVSKNFISQLFSFDVKFFDKYLLQLDTFDNVIPLFSRKQ"
     gene            1991..3049
                     /locus_tag="AC1PIW_001711"
     CDS             1991..3049
                     /locus_tag="AC1PIW_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HIP6606607.1"
                     /translation="MNKINSYKIHLPSLLPFGFLFANNCYTYREVFMEGQFEAVVEVD
                     EAGQLSSYVWDCEMEEVYTAHLVTAPSGAFVGQVRESYQSILDRVEEACCIALPFTKD
                     QSNRIAQLIKEQWGDLPDYPFAKLPTYGAFRHPNNNKWYALVSQIPRDKLDGSGSQEE
                     VEIVNLKVAGREIAELLSRSGIFPAYHMSKKTWVSVLLDDTVEDQTVFALLEKSRYLV
                     GPKSYKATQGPDYWVIPANPKVYDIDTEFAENKVVYWPQKSTIQVGDIVAIYVTAPVQ
                     AIRYVCRVLGENLENHGESDIPTEKKLMQVELLAQFSDDVLPRARMMDLGVKAVRGPR
                     RLTKELIDVLTSEVINIY"
     gene            3133..4125
                     /gene="asnA"
                     /locus_tag="AC1PIW_001712"
     CDS             3133..4125
                     /gene="asnA"
                     /locus_tag="AC1PIW_001712"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HIP6606608.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEIVEVQGPILSRVGDGM
                     QDNLSGVENAVTVNVKLIPDATYEVVHSLAKWKRHTLARFGFHEGEGLFVHMKALRPD
                     EDSLDPIHSVYVDQWDWEKVIPDGRRNLAYLKETVEQIYKAIRLTELAVEARYDIESV
                     LPKKITFIHTEDLVKNFPDLTPKERENVVAKEYGAVFLIGIGGELADGKPHDGRAPDY
                     DDWTSPSEAGYKGLNGDILVWNEALGSAFELSSMGIRVDEEALKRQVAITGDEDRLEF
                     DWHRALLNGLFPLSIGGGIGQSRLAMFLLRKKHIGEVQSSVWPQEVRDNFENIL"
BASE COUNT         1282 a          729 c          950 g         1241 t
ORIGIN      
        1 tttctttaaa aaaagaactg tatttgtatt aaaatttaat attttgtata aaaaatatga
       61 taaaataatg agaaatggag gtgcatatgg aaaaagaagt gtttactccc gatcaaagtc
      121 aatctattgt agatgctata ttgaagggat acaagtccta tcttgatgag cgaattgaaa
      181 agagaagaac aatgcatgtt tctgctggtt atgtttggac taaagctaat catatagatg
      241 gtgctcttgc gactgcaaac ctaaatttta ttgataaatt tgagttgaaa catgcgagtc
      301 aatcttggga gtttttagaa tttaactcag aaaatcaact attaggacgt gtatgttttg
      361 ttttaaaagg tcttactcgc ttgcggcagg tttttccaac agcagtaaaa agtggaaagg
      421 gctatttatt tgactatgct caaattaata caagttacct agaaaatctt tcaaggcata
      481 ccgatgaaag tacgcttttc cctgttcaga tggaattgtt tgatgacaat ttatttgaac
      541 ttttgaatcg gtctgaacaa tctatcaata actttttctt tatcacatat caggctgatg
      601 aattacatca cttgacgaat attcaagtta tcatgcctga ttcaagaact gggaaattga
      661 ttgaaattca aaatttatca acatttattt ctacaagtaa tgtcgatttt aacgatgaaa
      721 aatatcaaga gttacaaggc ttatcagaga ttgatgatgt agctgatttt gagattatac
      781 cacgtcttgc ttcagaaaaa gaagcttaga aaggcaaaaa aatgttcaat ggttcaagac
      841 tggaagaatt acgacttctg aatggcctta cacgtgcaga tttggcaaga gagcttgaag
      901 tgaccgaaca ggctatatgg caatttgaaa ctggagttac acttccaaaa atgaaaaata
      961 agatagcaat ggctcgtttt tttcaggttg aagttgacta ttttgatgct gttgataaga
     1021 atggttcatt ctcactaagt cgcattgcct ttcggaatgc agaccttgag gcaaaaaaaa
     1081 acatccatat ccaaatggta tatttggaaa aaatgaatca aatgatagat tatttggaga
     1141 actttgtatc cattcctaat caaataattt accagttagt tgatagtatt gagcaaaaac
     1201 ttcagcaaca tgaatcactt gagtttattg ctgatcatgt tagagaggta ttggcagtat
     1261 cagttgataa tgccaatctc ctctatagtt tagaaaaatc tgggatatac atttcagaga
     1321 gattaatcaa tggaaatgcg gatgcctata gtgcttggtc acgtaatggt cgacctttta
     1381 tcgttttggg actgggaaaa tcagcggtac gacataattt tgatttagca catgaactag
     1441 ggcatctact cttgcacaac caagtagatt ttgaggaact gtcaaaagaa gaattagagg
     1501 aaaaagagaa agaagccaat caattcgcct cctatttcct gttacctagg aaacaatttt
     1561 taatggattt tgtgaatatt gtaggtaagc gtgtcagcaa tcctgatcaa tatatcttgt
     1621 taaaaaaacg ttttggtgtc tcaattcaag cgcttgaata tagagcctac aagcttggct
     1681 tactgactcc acagcaaaat tcttatttct atcggcagat ttcaaaaaaa ggatataaag
     1741 cagttgagcc actagatttg gaaattccct taaagaaacc aagtaaaatc agaagtatct
     1801 tagatgtcat cctttcaaac aatcttttaa ctctgtcaga tattgaacga aatcagaggg
     1861 tgtcaaagaa ttttatcagt caattatttt catttgatgt gaaatttttt gataagtatt
     1921 tgcttcaatt agatacgttt gataatgtaa ttccattgtt ttctagaaaa caatgatggg
     1981 gagtagagta atgaacaaaa tcaattctta caaaatccac ctcccctctc tcctcccttt
     2041 cggtttcctt ttcgccaaca attgttacac ctaccgagag gtgtttatgg aggggcagtt
     2101 tgaggcggtg gtggaggttg atgaggctgg tcagctgtcg tcctatgttt gggattgtga
     2161 gatggaggag gtctataccg cccatctggt gactgcgcca tctggggctt ttgtggggca
     2221 ggtgagagaa agctatcagt ccattttgga tcgagtagag gaggcctgct gtatcgctct
     2281 accatttacc aaagaccaaa gcaaccgcat agcccagctc attaaggagc agtggggcga
     2341 ccttcctgac tatccctttg ccaagttgcc gacctatggg gcttttcgcc accccaacaa
     2401 taacaagtgg tacgccctgg tcagtcagat tccgagggac aagctggatg ggagcggttc
     2461 tcaagaagag gtggagattg tcaatctcaa ggttgccggt cgggaaatag cagagctgtt
     2521 gagtcggtcg ggcatcttcc ctgcctacca tatgtcgaag aagacctggg tgtcagtctt
     2581 gctggatgat acggtggagg atcagacggt gtttgctctc cttgagaaga gccgttatct
     2641 ggtaggacca aaatcctaca aggcgacgca agggcctgat tactgggtta ttccagccaa
     2701 tcccaaggtc tatgatatcg ataccgaatt tgcagaaaat aaggtagtct attggccaca
     2761 aaaatcaacc attcaagtcg gggacatcgt tgccatttat gtcacggcac ctgtacaggc
     2821 tattcgctat gtttgtcgag tcttggggga aaacttggaa aatcatggag agtcagatat
     2881 tccaacagaa aagaaactca tgcaggtgga attgcttgct cagttttccg atgatgtttt
     2941 gccgagagcg cgcatgatgg acctaggagt gaaagctgtc cgtggaccta gacgcctcac
     3001 aaaggagttg atagacgttc tgacatcaga agtgataaat atttattaaa ttatcagaat
     3061 atttaataaa aatctttaca tctgcaaaaa aagcggtata ataagactat taaaatttaa
     3121 ggaggagtag gtatgaagaa aagcttcatt catcagcaag aggagatttc ttttgtcaaa
     3181 aatacattta cccagtattt aaaagataag ctagaaatcg tagaagtgca aggaccgatc
     3241 ttgagtcgtg tcggggatgg tatgcaggat aatttgtccg gtgtggagaa tgcagttacc
     3301 gttaatgtga agctgatacc agatgctact tatgaagtgg ttcattcgct tgcaaaatgg
     3361 aagcgtcata ccttggctcg ctttggattt cacgaaggag aaggtctttt tgtacatatg
     3421 aaagccttgc gtccagatga ggattccttg gatccgattc actcggtgta tgtggatcag
     3481 tgggactggg aaaaggtcat ccctgatggt cgtcggaatt tagcttactt gaaagaaaca
     3541 gtagagcaga tttacaaggc tatccgtctg accgaattgg ccgttgaagc tcgttatgac
     3601 attgagtctg ttttaccaaa gaaaatcacc tttattcaca cagaggactt ggtgaagaac
     3661 ttcccagact tgacaccaaa agagcgtgaa aatgtggttg ccaaagaata cggtgcggtt
     3721 ttcctaatcg gtattggtgg agaactggca gatgggaaac cacatgatgg acgtgcacca
     3781 gactatgatg actggacaag cccatcagaa gcaggctaca aaggactcaa tggagacatc
     3841 ttggtttgga acgaagcact tggatcagcc tttgagttgt cctcaatggg gattcgtgtg
     3901 gatgaagaag cgctgaagcg ccaggttgct attacaggtg acgaggaccg tttggaattt
     3961 gattggcatc gtgctctttt gaatggtctc ttcccgcttt caatcggtgg cggtatcggt
     4021 caatcccgtc tggccatgtt cttgcttcgt aagaaacata ttggtgaagt gcagtcgagt
     4081 gtttggccac aagaagtgcg cgataatttt gagaatattt tataagagat tggcttcaat
     4141 ctcttttctt ctgctttcaa tctgctatct gaaaacattt acaacaagat tatctgtgat
     4201 tt
//
LOCUS       DBNFCK010000127         4178 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.127, whole genome shotgun sequence.
ACCESSION   DBNFCK010000127 DBNFCK010000000
VERSION     DBNFCK010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.127"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            52..549
                     /locus_tag="AC1PIW_001713"
     CDS             52..549
                     /locus_tag="AC1PIW_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HIP6606609.1"
                     /translation="MEKKIFQLLEWMASKTGQLVMGSFILLSVVTFSIFTIWDIAAAP
                     FNNARSHAVGVATEYADLQTVNDFSIYNGTETYFCVFGVTSQGEEVAVLIPEASSTVY
                     VYSLAQGISQEEAQAIAKKNGASQVERTILGLRDGKPIWEVKSGTAYYLVEFETGNFI
                     KREGL"
     gene            546..1727
                     /locus_tag="AC1PIW_001714"
     CDS             546..1727
                     /locus_tag="AC1PIW_001714"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002267262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIP6606610.1"
                     /translation="MSKLSKRVLEMEESVTLAAGARAKALKAEGRDILELTLGEPDFV
                     TPKNIQEAAIKSIEDGKASFYTVASGLPELKDAVNTYFENFYGYSIERNQVVLATGAK
                     FILYAFFAAVINPGDEVLIPTPYWVSYADQIKMNDGVPVFVTASEEHNFKVTVDQLEA
                     ARTDKTKVFLLNTPSNPTGMIYSREELEAIGNWAVEHDILILADDIYGRLVYNGNTFT
                     PISSISESIRQQTIVVNGVAKAYAMTGWRVGFAVGNPEIIAAMSKIVGQTTSNLTTAA
                     QYAAIEAFTGPQDTVETMRQAFEERLNTIYPLLAEVPGFEVIKPEGAFYLFPNVKKAM
                     EMKGYTDVTEFTTAILEEVGVAMVTGAGFGAPENIRLSYATDMDTLKEAVARLHTFMK
                     K"
     gene            1742..3088
                     /gene="asnS"
                     /locus_tag="AC1PIW_001715"
     CDS             1742..3088
                     /gene="asnS"
                     /locus_tag="AC1PIW_001715"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HIP6606611.1"
                     /translation="MSRKLITINQVKDYVGQEVTIGAWVANKSGKGKLAFLQLRDGTA
                     FFQAVAFKPNFIEKFGEEAGTEKFDVAKRLSQETSVYVTGIVKEDERSKFGYELDVTD
                     LEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAIQQIRNAIIYATYEFFEKNGF
                     IKFDSPILSGNAAEDSTELFETDYFGQLAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSFLSHEESLDLQEAYVKALIQGVIDRAPQALETLERDVD
                     ALKRYIAEPFKRVAYDDAITLLQEHEADKDTDYEHIEHGDDFGSPHETWISNYFGVPT
                     FVVNYPASFKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREDNYDALVAKMDEL
                     GMDKSEYEFYLDLRKYGSVPHGGFGIGIERMVTFVAGTKHIREAIPFPRMLHRLHP"
     gene            3360..3587
                     /gene="relB"
                     /locus_tag="AC1PIW_001716"
     CDS             3360..3587
                     /gene="relB"
                     /locus_tag="AC1PIW_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HIP6606612.1"
                     /translation="MGIVSLRLNDAEEEIFRSYAIHTGKSLSELFKTALAEQIEDQLD
                     YEIGIKALKKFQENPVTHSIDDVLAELEDGL"
     gene            3577..3846
                     /locus_tag="AC1PIW_001717"
     CDS             3577..3846
                     /locus_tag="AC1PIW_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE family
                     toxin"
                     /protein_id="HIP6606613.1"
                     /translation="MAYKLVLSDDALKQLKKMDRHVGMMLAKDLKKRLDGLENPRQFG
                     KALVGDYKGLWRYRVGNYRVICDIIDNKMVILALEIGHRKEIYKK"
BASE COUNT         1181 a          820 c         1001 g         1176 t
ORIGIN      
        1 atcacagaaa gaatggaaat atgctttaat ttgtagtata atgggactgt tatggaaaaa
       61 aagatttttc aactgttgga gtggatggct tccaagactg gtcagttagt gatgggctct
      121 tttatccttc tcagtgttgt gactttttca atttttacta tttgggatat tgctgctgcc
      181 cctttcaaca atgcgcgtag tcatgcagtt ggagtagcta cagaatatgc tgatttgcaa
      241 actgtcaatg atttttctat ctacaatggt actgaaacct atttttgtgt atttggtgtc
      301 accagtcagg gagaagaggt tgcagttttg attccagagg cttctagcac agtatatgtc
      361 tattctttag ctcagggaat ttcgcaagaa gaagcgcaag ctattgccaa gaaaaacggt
      421 gcaagccaag tagagcgaac tattctaggt ttaagggatg gcaaacctat ttgggaagtc
      481 aagtcaggta ctgcctacta tttagttgaa tttgaaacag ggaactttat caaacgggag
      541 ggcttatgag caagctatca aaacgtgtct tagaaatgga agaaagtgtc acgctggcag
      601 cgggtgcacg ggcaaaggcc ttgaaggcag aaggacgtga tattttagag ctgaccttgg
      661 gtgagccaga cttcgttaca ccgaaaaata ttcaagaagc tgctattaag tctatcgaag
      721 atggtaaggc gagtttttat acggtagctt caggtctgcc agaattgaag gacgcggtca
      781 atacctactt tgagaacttt tatggctact ctatcgaacg caatcaagtc gttcttgcca
      841 ctggtgccaa gtttatcctt tatgctttct ttgcggcggt catcaatcct ggtgatgagg
      901 ttctgattcc cacaccttac tgggtttctt atgcagatca aatcaagatg aatgatgggg
      961 tgcctgtctt tgtcactgca agtgaagagc acaatttcaa ggtaacagtt gaccagctgg
     1021 aagcagctcg gacagataag accaaagtat tcctactcaa tactccttct aatccgactg
     1081 gcatgattta cagtcgtgag gaattggaag ctattggcaa ctgggctgta gagcacgaca
     1141 ttctcatctt ggcagacgat atttacggtc gtttggtata taatggaaat accttcacac
     1201 cgatttcaag catttcagaa agcattcgtc agcagactat tgttgtcaat ggggtggcca
     1261 aggcctatgc catgacaggt tggcgggttg gatttgccgt tggtaatcct gagatcattg
     1321 cggctatgag taagattgtc ggacaaacga cgtcaaactt aacaaccgct gctcagtatg
     1381 cggccattga agcctttaca ggtccgcaag atacggtcga aaccatgcgt caggcatttg
     1441 aagagcgact caataccatt tatcccttgc tggcagaagt gcctggtttt gaagtgatta
     1501 agccagaagg tgccttctat ctcttcccaa atgtcaaaaa ggctatggaa atgaagggct
     1561 acacagatgt aactgaattt accacagcca ttttggaaga agtgggagtt gcaatggtga
     1621 caggtgctgg ttttggagct cctgagaata tccgcctcag ctatgcgaca gatatggaca
     1681 cgctcaaaga agcagtggct cgactacaca catttatgaa aaaataaaaa gaggaaaaat
     1741 aatgtctaga aaattgatta ccatcaacca agtaaaagat tatgtcggtc aagaagtgac
     1801 cattggtgcc tgggttgcta acaaatcagg caagggcaag ttagccttcc tgcaattgcg
     1861 tgacggaaca gccttcttcc aagcagttgc cttcaaacca aacttcatcg aaaaattcgg
     1921 tgaagaagct ggtactgaga agtttgatgt agcaaaacgc cttagccaag aaacgtctgt
     1981 ctatgtgaca gggattgtca aggaagatga gcgttctaag tttggttatg agttggatgt
     2041 gacagacctt gaagtgattg gtgagtcaca agattaccca atcacaccaa aagaacacgg
     2101 tacggacttc cttatggaca accgtcacct ctggttgcgt tctcgtaaac aggtcgctat
     2161 ccagcaaatc cgtaacgcca tcatctatgc gacttatgaa ttctttgaaa agaatggctt
     2221 tatcaagttt gatagcccaa tcttgtctgg aaatgcggca gaagattcga cagaattgtt
     2281 tgaaacagac tactttggtc agcttgccta cctcagccaa tctggtcagc tttacctgga
     2341 agctggtgcc atggcccttg gtcgtgtttt cgacttcggt cctgttttcc gtgcggaaaa
     2401 atcaaaaact cgccgtcact tgacggagtt ctggatgatg gatgccgagt attcattcct
     2461 cagccatgaa gagtcacttg acttgcaaga agcttatgtc aaagccttga ttcaaggtgt
     2521 tatcgaccgt gctccacaag ccttggaaac tcttgagcgt gatgtggatg ctctcaaacg
     2581 ctacattgca gagccattca aacgtgtggc ttatgatgat gctattaccc ttcttcaaga
     2641 gcatgaagct gataaagata cagactacga acacatcgag catggtgatg actttggatc
     2701 accacatgaa acttggattt caaactactt tggtgtaccg actttcgttg ttaactatcc
     2761 agcaagcttc aaggccttct acatgaagcc agttccaggt aatccagagc gcgtgctttg
     2821 tgcggacctc ttggcaccag aaggctatgg tgaaatcatt ggtggttcta tgcgtgagga
     2881 taactacgat gccttggtag ccaagatgga cgagctcggt atggacaagt cagagtacga
     2941 attttacctt gaccttcgta aatacggttc agtgccacac ggtggtttcg gtatcggtat
     3001 tgagcgtatg gtaaccttcg ttgctggtac aaaacacatc cgtgaagcca ttccattccc
     3061 acgtatgttg caccgcttgc atccgtaata cagtcgttca gtttttcctt tattacgtcg
     3121 acgtatgagg aaactccgtt tccttatttc caagttcaaa taatctcatc attatttgaa
     3181 ctcgttttgc cgtaccttag tacagtctgc agctcgttgc ctagtactaa aagtaaactg
     3241 aaagactgcg aaataaatat tcactgacag actacaaacg tggtctgttt ttctggtgtc
     3301 aattgacatt ttgaacgaat tgttttacaa tgtattacaa gaagaaagta ggtggaatga
     3361 tgggaattgt atccttgcga ttaaacgatg cagaagaaga aattttcaga agctatgcta
     3421 ttcatacagg aaaaagcctg tctgagctat tcaaaactgc acttgctgaa cagatagaag
     3481 accagttgga ctatgaaatt ggtatcaagg ctttgaagaa attccaagaa aatcctgtta
     3541 cacactcgat tgatgatgtt cttgcggagt tagaagatgg cttataagct tgttctcagc
     3601 gatgatgctt tgaagcagtt gaaaaaaatg gacaggcatg tggggatgat gcttgctaag
     3661 gatttgaaaa aacgtttgga cggtttggag aatccaagac aatttgggaa ggccttggta
     3721 ggagactata aggggctgtg gcggtataga gtggggaatt accgtgtaat ttgtgatatt
     3781 attgataaca aaatggtcat cctggccctt gaaatcgggc accggaaaga aatctataaa
     3841 aaatgaagtt ctaggtaact aggctcggta tcggtgtact tctcagagtt cgtgtcaaca
     3901 tctcagcgca gtggttgatt ggctttaaca gtccagtgga gtgaagcagt tctgggaact
     3961 gtttcagccc gagtcttgaa gcagaaaagc taggtaaaaa ggttggagat aaggtttgcg
     4021 aagcaaatct cagcaattcg tgttttccac tccaaatctg acctatacga ctgatgcgaa
     4081 ctttgttcgc cttatctcta acctccaaag gttccctgaa actttggagc tatgcggggg
     4141 tggagagatg aactcctttt tgacttatcg agttcttc
//
LOCUS       DBNFCK010000128         4080 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.128, whole genome shotgun sequence.
ACCESSION   DBNFCK010000128 DBNFCK010000000
VERSION     DBNFCK010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.128"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            134..346
                     /locus_tag="AC1PIW_001718"
     CDS             134..346
                     /locus_tag="AC1PIW_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HIP6606614.1"
                     /translation="MSEEKFDAKLEQLSGSAKEVIGKLTGDKEIEVEGLVEKGIGKAK
                     ELVEDAKDGLEGAINGIKNAFDKEEK"
     gene            516..950
                     /locus_tag="AC1PIW_001719"
     CDS             516..950
                     /locus_tag="AC1PIW_001719"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HIP6606615.1"
                     /translation="MKKIVFVCLGNICRSPMAEFVMKDMTSQFHIESRATSNWEHGNP
                     IYPGTQKIFQKHKISYDQDKSSQQMSQTDFEAFDIIIGMDSNNVRDLQKIAPAHAQNK
                     IFQFAEKSVPDPWYTGDFDETYEMVKKGCQDWLDRLQSFDQE"
     gene            1082..1504
                     /locus_tag="AC1PIW_001720"
     CDS             1082..1504
                     /locus_tag="AC1PIW_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606616.1"
                     /translation="MKEFKLPSIKWHDITSHFTRPKLEILSLIIILICALSVFTGRIA
                     SKQAMTFNNGALQYNGYVVANKMNGQGKLTFDNGDVYEGQFTNGIFHGQGTYTSASGW
                     VYTGQFKNGYADGKGKLTTEGQATYEGTFKQGIYQYEN"
     gene            1494..3293
                     /locus_tag="AC1PIW_001721"
     CDS             1494..3293
                     /locus_tag="AC1PIW_001721"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIP6606617.1"
                     /translation="MKIKWLSVIRVIGLVFVLLYHFFIKYFPGGFVGVDLFFTLSGYL
                     TTALLIDEFAKHKKIDIVSFFRRRFYRILPPLVLTILLVLPLALLVRNDFIANIGNQI
                     VAALGFMTNFFEILSGGSYENQFSPHLFVHTWTLAIEVQYYLVWAGLVWYRTRISKSV
                     GQLRGSIFLTSISLFSLSFLAMSISSFFVDNFSTIYYSSFTHTFPFFLGSILATIAGI
                     GNTTTAFQQQVNTLSIKQAIGLFLGGLGVELLLLFTLQFSSIWTYLIGFLLSSLATAT
                     MIFAARVLHEKTEDVKEPAIILFFASISYGIYLFHWPLLIIFTQLTNATIAAGLSFVF
                     STILSTISFYILEPYIAGKVGSLFGLAIDLKPYKKWLLSSFACLTIVGLGICLFGPKL
                     GSFEQESMVSNLYQAQTQLNTTRSAAENAKATSFEIQKGVIIFGDSVTVRASSAIQTA
                     LPDAQIDGTVSRNLSEIAKLINLYKQNNSLKETVVVALGTNTTDNYQELLDQLVKDFP
                     KGRRLIFVTPYDGNFTPSESISYQTGQYEKELAEKYDYISIADWYQVAKDNPPIWYNT
                     DLVHFNLESNGGEVFAQTIKNAVEAVADGPIKN"
     gene            complement(3520..>4080)
                     /locus_tag="AC1PIW_001722"
     CDS             complement(3520..>4080)
                     /locus_tag="AC1PIW_001722"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIP6606618.1"
                     /translation="LAGFDSTVQSAIRGNLPSGASQFWTSITVLGNTVIILAITMILA
                     AIFYYYKKWKIEAYFLLASFAAMGVASTALKYVYQRPRPSIEWLIDTIGYSFPSWHTA
                     STMMIGGAVVIIVHQRMKKGIARLVLQAMLILLAVLVAVSRIYIGVHYPTDIIGGWLL
                     AVTLLQMMYPFYDQKRFEWRFQSKQK"
BASE COUNT         1291 a          804 c          771 g         1214 t
ORIGIN      
        1 ctcctctgaa ttattggcag acaaattcct tgctatatta acttatatac gttagactag
       61 taagtaagaa tagtatgtga ttttcttttt agaaaatcat cgcccccata tgtgtatatc
      121 gaggagggat tttatgtcag aagaaaaatt tgacgcaaaa ctagaacagc tatctggatc
      181 tgcaaaagag gtaattggta agctgaccgg cgacaaggaa atcgaagttg aaggacttgt
      241 tgagaaagga attggcaaag caaaagagct agtcgaagat gcaaaagatg ggcttgaagg
      301 tgccattaac ggcatcaaaa atgctttcga caaagaagag aaataaacaa agagttcaat
      361 ataggtagga ttggtcgtgc actgtttgat aatgaacatt acacttaatt tggtctttta
      421 cctatagccc gaacaaaaat atgcaagaga ggggaactca gacatttacc tgtcgagtct
      481 accttctctt ttcttatgtc acgaaaggag tatctatgaa aaaaatcgtt tttgtctgtc
      541 ttggaaatat ttgtcgtagc cctatggctg aatttgtcat gaaagacatg acaagtcaat
      601 ttcacattga aagccgtgct acttcaaact gggaacatgg taatcctatc taccctggaa
      661 cacagaaaat tttccaaaag cacaagattt cctatgacca agataaatca tcacagcaaa
      721 tgagccagac agatttcgaa gcatttgata ttatcatcgg tatggacagc aataatgtcc
      781 gagatttaca gaaaatagca ccagcacatg cccaaaataa gatttttcaa ttcgcagaaa
      841 aatccgtacc agacccttgg tatactggag attttgacga aacctacgaa atggtaaaga
      901 aaggttgtca agactggttg gacaggttac agtcatttga tcaagaataa gattttctat
      961 gtaatatttt tgtaatattt gtaaaaaaat ggtaaactat ttctgactga actgataatt
     1021 tcttactgta tcctagaaag aactgattag catcggtcaa atcctatgaa agagtaaaac
     1081 gatgaaagag ttcaaattac ctagtataaa atggcatgat ataacaagtc atttcacccg
     1141 tccaaaactt gaaatcctct cccttattat tatcctcatc tgtgccctct ctgtatttac
     1201 agggcgaatt gccagcaagc aagccatgac tttcaataat ggcgccctgc agtacaacgg
     1261 ttatgtggtc gcaaataaaa tgaatggtca aggaaaattg acctttgata atggagatgt
     1321 ctacgaagga caatttacaa atggaatttt ccacggtcaa ggaacctaca catctgcaag
     1381 tgggtgggtt tataccgggc aatttaaaaa cggctatgcc gatggaaaag gaaaattgac
     1441 caccgaagga caagctacct acgaaggaac ttttaaacag gggatttatc aatatgaaaa
     1501 ttaaatggtt atccgttata cgtgtgattg gattagtctt tgtcctactc tatcatttct
     1561 ttatcaaata tttcccaggt ggctttgtgg gggttgacct cttctttacc ctgtctggat
     1621 atttgacaac cgcccttttg attgatgagt ttgcaaaaca taaaaaaata gatattgtca
     1681 gctttttcag aagaaggttc tatcggattc tcccccctct ggttttaacg attcttttgg
     1741 ttcttccact tgctctgctt gtccgcaatg atttcattgc caatatcggc aatcagatag
     1801 tcgctgcgct cggattcatg acaaacttct ttgaaattct atcaggtggt agttacgaga
     1861 atcaattttc acctcatcta tttgtgcata cttggacact agccatagaa gttcagtatt
     1921 accttgtctg ggctggttta gtttggtatc ggactcgcat ctcaaaaagt gtggggcaac
     1981 tacgtggatc aatttttcta acatctataa gcctcttctc gctaagtttt ttagcaatgt
     2041 ctatctcaag tttctttgta gataatttct caaccattta ctactcgagc ttcacacata
     2101 ccttcccatt cttcctcgga agcattctcg caaccatcgc tggtatcgga aatacaacaa
     2161 ctgccttcca gcaacaagtg aatacattga gtatcaaaca agctataggt ctgtttcttg
     2221 gaggactagg agtagaactt cttcttctct ttactcttca attcagttcc atctggacct
     2281 atctaattgg ttttcttcta tctagtttag ctacagcaac tatgattttt gctgctcggg
     2341 tacttcacga gaaaaccgaa gatgtcaaag aacctgcaat tatcctcttc tttgcaagta
     2401 tttcttatgg tatctacctc ttccattggc ccttgctcat tatctttaca caactgacaa
     2461 atgccactat cgctgcggga ttatcctttg ttttttctac tatcctatca actatttcct
     2521 tctatattct agaaccatat attgctggta aagtaggaag cttgtttgga ttagcaatag
     2581 acttaaaacc ctataaaaaa tggcttctta gcagctttgc ttgcttgacc atagttggct
     2641 taggtatctg tctatttgga ccaaaattag gaagttttga acaagagagc atggttagca
     2701 atctttatca agctcagacc caactaaata caacacgatc tgctgctgaa aacgctaaag
     2761 ctactagttt tgaaattcaa aaaggagtta tcatctttgg cgattctgtt actgttcgcg
     2821 ctagttctgc catacagacc gcgctaccag atgcacagat cgatggaact gtcagtcgaa
     2881 atctctctga aattgctaaa ttgataaatc tttataagca aaataatagc ttgaaagaga
     2941 ctgtagttgt agcgctaggt acaaatacta cggataatta tcaggaacta ctagaccaac
     3001 tagtcaaaga ttttcctaaa ggacgtcgcc ttatttttgt cactccttac gatggaaact
     3061 tcactccgag cgaatccatt tcttatcaaa ctggtcaata cgagaaagaa ttggctgaaa
     3121 aatatgatta tatttccatt gcagactggt atcaagttgc aaaggataat ccaccaattt
     3181 ggtacaatac cgaccttgtt catttcaatc ttgaaagcaa tggaggcgaa gtcttcgccc
     3241 aaacgataaa aaatgctgtt gaagccgttg ctgatggtcc aatcaaaaac tagatttata
     3301 catcatcatc ctctgagaat ttctcggagg atttttgcac gattttccga taagtccagt
     3361 gcgaactcta agaaatggcg ctgtgcctga gctcagtctc aataaatctt gtgaaataag
     3421 ccacttctac aaaggacgga aaagcgaagt aacgataata gaaagatgac aagttaaaaa
     3481 aactcctacc ttttacagta ggagttggtg tcaaattgac tatttttgct tactttggaa
     3541 acgccattca aagcgtttct gatcgtaaaa tggatacatc atttgtaaaa gagtgacggc
     3601 aagtagccaa ccaccgataa tatctgtcgg gtagtgaacc ccaatataga tacgggatac
     3661 agctaccagg acagcaagta gaatcaacat ggcttgtagc acaagtctag ctatgccttt
     3721 cttcatcctc tggtgaacaa taataacaac tgctcctcca atcatcatag ttgatgcagt
     3781 atgccagcta ggaaatgaat aaccgatggt atcaatcaac cattcaatac ttggacgcgg
     3841 acgttggtag acatatttca gggctgtgga tgccacaccc atcgccgcaa aactagcaag
     3901 taggaaataa gcttctatct tccatttttt atagtagtaa aagatagcag ctagaatcat
     3961 ggtaatagct aaaataataa cagtatttcc taaaacagta atagaggtcc aaaattgact
     4021 ggctccactt ggtaaatttc cacgaatggc agactggaca gtcgaatcaa aacctgctag
//
LOCUS       DBNFCK010000129         4045 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.129, whole genome shotgun sequence.
ACCESSION   DBNFCK010000129 DBNFCK010000000
VERSION     DBNFCK010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.129"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            220..438
                     /gene="nrdH"
                     /locus_tag="AC1PIW_001723"
     CDS             220..438
                     /gene="nrdH"
                     /locus_tag="AC1PIW_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HIP6606619.1"
                     /translation="MVTIYSKNDCVQCKMTKKFLDQHSVDYKEINLDEQPEFIDHVQG
                     LGFSAAPVIETENDVFSGFQPGKLKALV"
     gene            471..2630
                     /gene="nrdE"
                     /locus_tag="AC1PIW_001724"
     CDS             471..2630
                     /gene="nrdE"
                     /locus_tag="AC1PIW_001724"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HIP6606620.1"
                     /translation="MSLKELGDVSYFRLNNEINRPVNGQIPLHKDQEAVKAFFTENVL
                     PNTKQFDSILDKIAYLLEENYLEKEFLDQYSPEFIIKIDQFLKDQNFRFKSFMAAYKF
                     YNQYALKTNDGAYYLESMEDRVLFNALYFAEGDEELALNLANEMIHLRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGISLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLDVFHPDIISFLSTKKENADEKVR
                     VKTLSLGITVPDKFYELARKNEDMYLFSPYSVELEYGVPYSYLDITEKYDELVANPRI
                     RKTKIKARDLETEISKLQQESGYPYVINIDTANRSNPVDGKIIMSNLCSEILQVQTPS
                     ILNDSQEYLSMGTDVSCNLGSTNIVNLMKSPDFGRSVRTMTRALTYVTDHSHISAVPS
                     IEAGNSQAHSIGLGAMGLHSYLAQNLIDYGSKTAVEFTNIYFMLLNYWTLVESNNIAR
                     ERKATFHNFDKSKYADGTYFDKYVTGDYQPQSDRVKELFEGIFIPSGQDWAELREKVM
                     ADGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLTTETI
                     PFYKSAYDMDMRKVIDVYAAATEHVDQGLSLTLFLRSELPKELYEWKKENKQTTRDLS
                     ILRNYAFNKGIKSIYYIRTFTDDGEEVGANQCESCVI"
     gene            2676..2870
                     /locus_tag="AC1PIW_001725"
     CDS             2676..2870
                     /locus_tag="AC1PIW_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606621.1"
                     /translation="MFGFGKTKQENKKLKRENNVLKKAYSQTKKENNKLKKTSDRLAA
                     DGLRHGSPEAGKYLVGKRKK"
     gene            2897..3856
                     /gene="nrdF"
                     /locus_tag="AC1PIW_001726"
     CDS             2897..3856
                     /gene="nrdF"
                     /locus_tag="AC1PIW_001726"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HIP6606622.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLTAEEKDLVGKVFGGLTLLDTLQSETGVQALRNDIRTPHEEAVYNNIQFMESVHAK
                     SYSSIFSTLNTKSEIEDIFEWTNSNEYLQRKAKIINEIYETGTPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELSEEEQDKLR
                     DWMYDLLYQLYENEEGYTRSLYDAVGWTEEVLTFLRYNANKALMNLGQDPLFPDSADD
                     VNPIIMNGISTGTSNHDFFSQVGNGYLLGEVEAMTDDDYLYGL"
BASE COUNT         1265 a          892 c          771 g         1117 t
ORIGIN      
        1 gttatcaaac taaatagaaa cagctatgta aacgctattt ttcgacttgg cagcattgga
       61 cggatgtaca atatatggtg tcttatgaaa atattgtgca ctatattttg tgattttaaa
      121 taaacttcct tcttgacttt aaccttaaaa tcttgtatcc tagattggta acaaaattgg
      181 gattttttat taatccctca tggacaaagg agaaattgaa tggtaacgat ctattcaaaa
      241 aatgattgtg tacagtgtaa aatgaccaag aaattcttgg accaacatag tgtagattat
      301 aaagaaatca atctagatga acagcctgaa tttatcgatc acgtacaagg tctcggtttc
      361 tcagctgcac ctgttatcga aactgaaaat gatgttttct caggttttca accaggaaaa
      421 ctaaaagcct tggtttaact ccctactgtt tatagaaaga gattgcgact atgagtttaa
      481 aagagctagg cgatgtgtcc tacttccgtt taaataacga aatcaaccgt cctgtcaatg
      541 gacaaattcc tctccacaag gaccaagaag cggttaaggc cttctttaca gaaaacgtcc
      601 tcccaaatac caagcaattt gatagtattt tagacaagat tgcttatcta ttagaagaaa
      661 attacctcga aaaagagttt ttggaccaat acagtccaga atttatcatc aaaatcgatc
      721 aattcttgaa agaccaaaat ttccgcttca agtctttcat ggctgcttac aagttctaca
      781 accagtatgc ccttaaaacc aacgacgggg cttactactt ggaaagtatg gaagatcgcg
      841 ttctcttcaa cgccttgtat tttgctgagg gcgacgaaga attagcacta aacctggcca
      901 acgaaatgat tcatcttcgt taccagccag caacaccgtc tttcctcaac gcaggccgtg
      961 cccgtcgtgg tgagttggta tcgtgtttcc tcattcaagt cactgacgat atgaactcga
     1021 tcggacgttc tatcaactct gctctgcaat tgtcccgtat cggtggtggt gttggtattt
     1081 cccttagcaa cttgcgtgag gcaggtgctc caatcaaggg ctatgagggg gcagcttctg
     1141 gggttgttcc tgttatgaaa ctcttcgaag acagcttctc ctattctaac cagcttgggc
     1201 aacgccaagg ggctggtgtc gtttatcttg atgttttcca cccagatatt atttccttcc
     1261 tttcgactaa gaaagaaaat gccgatgaga aagttcgcgt gaagacctta tcactcggta
     1321 ttactgttcc tgataaattc tacgaattgg cacgtaaaaa cgaagatatg tacctcttca
     1381 gcccatactc agttgagttg gaatacggtg tcccttatag ctaccttgac atcacagaaa
     1441 aatacgacga gttggtagca aacccacgta tccgtaagac aaaaatcaag gcacgtgact
     1501 tggaaacaga aatttccaaa ctccaacaag agtctggcta tccatacgtt atcaacatcg
     1561 atacagctaa ccgtagcaac cctgttgacg gcaagattat catgtcaaac ctctgttcgg
     1621 aaatccttca agttcaaaca ccaagcattc ttaatgattc gcaagaatac ttgagcatgg
     1681 gtactgatgt ttcatgtaac ctcggttcaa ccaatatcgt taacttgatg aagtcgcctg
     1741 attttggacg ttctgtccgt accatgactc gtgctttgac ttatgtcaca gatcattcac
     1801 acatttcagc tgtaccatct attgaagcag gaaacagtca agcgcattca atcggtcttg
     1861 gagctatggg acttcattct tacttggctc agaacttgat tgattacggt tcaaaaactg
     1921 ctgtagaatt taccaacatc tacttcatgc tcctcaacta ctggactttg gtagaatcaa
     1981 ataacattgc ccgtgaacgc aaggcaacct tccataattt tgacaaatca aaatacgcag
     2041 acggcactta tttcgataaa tacgtgaccg gagactacca accacaatct gaccgtgtca
     2101 aagaactatt tgaaggaatt ttcattccaa gtggacaaga ttgggcggag cttcgtgaaa
     2161 aggtaatggc agatggtctc tatcatcaaa accgtctagc tgttgcacct aacggttcta
     2221 tctcttatat caacgacgtt tctgcttcaa ttcacccaat cacacaacgg attgaagaac
     2281 gccaagagaa gaaaatcggt aaaatctact atccagcagc tggcttgaca acagaaacca
     2341 ttccattcta taaatctgcc tacgatatgg atatgcgcaa ggtcattgat gtctatgctg
     2401 ccgcaacaga acacgttgac caaggtttgt ccctcaccct cttcctccgc agcgagctcc
     2461 caaaagaact ctatgaatgg aaaaaagaga acaaacaaac tacacgtgac ctctctatcc
     2521 ttcgtaacta cgccttcaac aaaggtatca agtctatcta ctacatccgt acctttaccg
     2581 atgacggcga agaagtcggc gcaaaccaat gtgaaagttg tgtaatctga tttacagact
     2641 aaaataaagc tattcaatat ttctggaggt tttttatgtt tggatttggt aaaacaaaac
     2701 aagaaaataa aaaattaaaa agagaaaata acgtactaaa aaaagcttat tcacaaacta
     2761 aaaaagaaaa taataagctc aaaaaaacga gcgaccgact agctgcggat ggattacgcc
     2821 acggtagtcc tgaagcagga aaatacttag tcggaaaacg caaaaaataa ttattgataa
     2881 aacgaaagaa agtaatatgg aaacctacta caaagccata aactggaacg ccattgagga
     2941 tgttattgac aagtcaactt gggaaaagtt gactgagcaa ttctggctcg atacacgtat
     3001 tcctttatca aacgacttgg atgactggcg taaacttact gctgaagaaa aagacctggt
     3061 tggtaaggtc tttggcggat tgaccctctt ggataccctt caatctgaaa caggtgtcca
     3121 agccctccgc aatgacattc gcacacccca tgaagaagct gtttacaaca atattcaatt
     3181 catggagtct gttcacgcca aatcttactc ttctatcttc tcaaccttga ataccaagtc
     3241 tgagattgaa gacatctttg aatggaccaa cagcaacgaa tatttgcaac gcaaggcaaa
     3301 gattatcaat gaaatctacg aaacaggtac accacttgaa aagaaagtag ccagcgtatt
     3361 ccttgaaacc ttcctcttct actctggttt cttcacgcca ctctactacc ttgggaataa
     3421 caaactggcc aacgttgcag aaatcatcaa actgattatc cgtgacgaat ctgtgcacgg
     3481 tacctacatt ggctacaagt tccagcttgg tttcaatgaa ttatcagagg aagaacagga
     3541 caaacttcgc gactggatgt acgacctgct ctaccaactt tacgaaaatg aagagggcta
     3601 tacacgctcc ctctatgatg cagttggctg gactgaggaa gtcttgactt tccttcgtta
     3661 caacgccaac aaagccctca tgaacttggg acaagatcca ctcttcccag attcagcaga
     3721 tgatgtcaac ccaatcatca tgaacggtat ctcaaccggt acatctaacc acgacttctt
     3781 ctcccaagtc ggtaacggct atcttcttgg tgaagtggaa gctatgacag atgatgatta
     3841 tttatatggg ttgtagatct taggaaaaat atcatttcta tttttccgtt ttatagtcat
     3901 tttgtttatc tttgacgtga ggctgggcaa aaagcccagc gccacttctt agagttcgtg
     3961 tcaacatctc agcgcagtgg ttgattggcg gatttgttcg tgttttacac tctaaatcca
     4021 acctaatcaa ctgtgcggca ctcca
//
LOCUS       DBNFCK010000130         3980 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.130, whole genome shotgun sequence.
ACCESSION   DBNFCK010000130 DBNFCK010000000
VERSION     DBNFCK010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.130"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            342..1037
                     /locus_tag="AC1PIW_001727"
     CDS             342..1037
                     /locus_tag="AC1PIW_001727"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HIP6606623.1"
                     /translation="MKLLITSGGTSEAIDQVRAITNHASGNLGKIIAEQALKRGHEVT
                     LVTTRQAVKPDSQKNLTIIEITNVDSLKETLEPLVKTHQALIHSMAVSDYTPVYMAGL
                     DELRATEDISSLLKKQNTESKISSKDDYQVLFLKKTPKVISYVKKWNPAITLFGFKLL
                     VNVPKEELFAVARQSIERNGADYILANDLKDIGENQHIAYLVDKTREIQAQTKDEIAQ
                     LILNTLEKGEQNG"
     gene            1030..1578
                     /gene="coaC"
                     /locus_tag="AC1PIW_001728"
     CDS             1030..1578
                     /gene="coaC"
                     /locus_tag="AC1PIW_001728"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HIP6606624.1"
                     /translation="MANITLAVTGSISAYKAADLTSQLTKIGHQVTVLMSRSAMDFIT
                     PLTLQSLSKNLVHTDVMIEENPTLIKHIDIAKETDVFLVAPASANTIAKLANGMADNI
                     ITATALALPIGTKKLLAPAMNTNMYLNPATQKNIKTLIEYGFEEIKPREALLACGDFG
                     TGALAEVTDILEKVSNTLDEKK"
     gene            1565..2134
                     /locus_tag="AC1PIW_001729"
     CDS             1565..2134
                     /locus_tag="AC1PIW_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIP6606625.1"
                     /translation="MKKNKSTQIATIAIFFAVMIVINILSSIIFNILPVPIKPTIVHI
                     PVIIASIIYGPRVGACLGALMGLMSMTHNTIILLPTSYLFSPFVENGNVYSIIIAVVP
                     RILVGITPYFVYKWIKNRKGLFIAGAIGSMTNTIFVLGGIFFLFSSVFDGNIQAMLAT
                     VLGTNSIAEMIISSVLTAALVPTLQKIQK"
     gene            2225..3943
                     /locus_tag="AC1PIW_001730"
     CDS             2225..3943
                     /locus_tag="AC1PIW_001730"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HIP6606626.1"
                     /translation="MTYQETYQTWLDFADLPDYLREELVAMDEKTKEDAFYTNLEFGT
                     AGMRGYIGAGTNRINVFVVRQATEGLAKLVESKGEEAKKRGVAIAYDSRHFSPEFAFE
                     SAQVLAAHGIKSYVFESLRPTPELSFAVRHYNAIAGIMVTASHNPKEFNGYKVYGEDG
                     GQMPPADADALTNFIRAIDNPFAVELADLEASKENGLITVLGEETDVKYLEELNDLNI
                     NPELIAEYGKDMKIVYTPLHGTGEMLARRALAQAGFESVQVVEAQATADPDFSTVASP
                     NPESQAAFALAEELGREVGADVLLATDPDADRVGVEVRQADGSYWNLSGNQIGAIIAK
                     YILEAHKQAGTLPANAALAKSIVSTELVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMFGFEESFGYLIKPFVRDKDAIQAVLMVAEIAAYYRSRGMTLADGIDEIFKE
                     YGYFAEKTISVTLSGKDGAEQIKAIMAKFRDNSPAQFNATDIAVFEDFALQTKTDKDG
                     NIEKLTTPPSDVLKYTLADDSWFAVRPSGTEPKIKFYIATVGETLAEAEEKISNIEKE
                     INEFVG"
BASE COUNT         1248 a          830 c          750 g         1152 t
ORIGIN      
        1 atattttttt actatttatt cactaacgta cggaattttc agaaaaacta caggtttcct
       61 tacttaaaca gatacaaatt tttaaaaact gcattctcat catagctcta cacttccatc
      121 taaggctggt tttagttagt tttttcaaaa gagagtcagt attcctttaa aaattgcctt
      181 gattaacaaa ccaacgcttt cttgcacaaa aacacttcac tatataatac aggcccttat
      241 attttttgta ttagcagcat cattttccag tgtcacagat tgattagcaa atagattagt
      301 acaaacttta cgaaaaatag aaattctagt aaaatatgaa catgaaacta ttgattacat
      361 caggcggaac aagcgaagct atcgatcaag tccgcgctat tacaaaccat gcttctggaa
      421 atcttggaaa aatcattgcc gaacaagcct tgaaacgcgg tcatgaagtt actcttgtga
      481 ctacaagaca ggctgttaaa ccagattctc agaagaattt aaccattatc gaaatcacaa
      541 atgttgatag tttgaaagaa acattagaac ctttagtcaa aacacatcag gctctgattc
      601 atagcatggc tgtatccgat tatacacctg tttatatggc tggtttagat gaactaagag
      661 caactgaaga catttctagt ctactaaaaa aacagaatac agaaagtaag atttcatcca
      721 aggatgacta ccaagttctt ttcttgaaaa aaactcccaa ggtcatttcc tatgtaaaaa
      781 aatggaatcc agctatcaca ctctttggct tcaaactatt ggttaatgtt cctaaggagg
      841 aattatttgc cgtagctcgc caaagcattg aacgaaatgg cgctgattat atacttgcta
      901 atgacttgaa ggatattgga gaaaatcaac acattgccta tttggttgat aaaacaaggg
      961 agattcaagc acagacaaaa gatgaaattg ctcagctcat tttaaatacc ctagaaaaag
     1021 gagaacaaaa tggctaatat cacacttgcc gttactggct ctatttcagc ctacaaagca
     1081 gcggacctaa ctagtcaatt gaccaagatc ggtcatcagg ttacagtcct catgagtcgc
     1141 tctgctatgg acttcatcac acctttgact ctccaatcct tgtcaaaaaa tctggtccac
     1201 acagatgtca tgattgaaga aaatcctact cttatcaagc atatcgatat tgccaaagaa
     1261 acagacgtat ttttagttgc ccctgcttct gccaatacga tagctaaatt agctaatggt
     1321 atggctgaca acattattac ggcaacagcg ctggctctgc caattggtac taaaaaactg
     1381 ctggcacctg ctatgaatac taacatgtac ctcaatcctg ctacccagaa aaatatcaaa
     1441 accctcatag agtatggttt tgaggaaatc aaaccgcgtg aagccctgct tgcttgtgga
     1501 gattttggaa ctggggcctt ggctgaggta actgatattt tagagaaagt gagtaacacc
     1561 cttgatgaaa aaaaataaat caactcaaat tgctacaatt gcaatcttct ttgctgtcat
     1621 gattgtcatc aatatattga gttcaattat ttttaatata ttgcctgttc caataaaacc
     1681 caccatcgta catatcccag tcattattgc ttctattata tacgggccac gtgttggggc
     1741 ttgtttgggg gctctcatgg gcttaatgag tatgacccat aatactatta tcttactacc
     1801 aaccagctat cttttctccc catttgttga aaatggtaat gtttattcaa tcatcattgc
     1861 agttgtccca cgtatattag tcgggattac accttatttc gtctacaaat ggataaaaaa
     1921 tcgtaaagga ttatttattg ctggtgcaat tggttcaatg accaatacta tttttgtact
     1981 tggaggtatt ttcttcctct tctcctctgt ctttgatggt aatattcaag caatgttagc
     2041 aaccgtttta ggtaccaatt caattgcaga aatgatcatt tcttctgtat taactgcagc
     2101 gcttgttcca actttacaga aaattcaaaa ataaatatca gaaaatcatg tgaaagcgtg
     2161 atttttcttt tcaaacatgg tataatgaaa gcgtttaata gaaaaatttt taaggagata
     2221 tgcgatgact tatcaagaaa cctatcaaac atggctcgac tttgcggacc ttccagatta
     2281 cttgcgtgaa gaattagtcg caatggatga aaaaacaaaa gaagatgcct tctatacaaa
     2341 tcttgaattt ggtacagctg gtatgcgtgg ctatattggc gctggtacca accgcatcaa
     2401 cgtatttgta gtccgtcaag ctactgaagg tttggcaaaa ttggtagaat caaaaggtga
     2461 agaagctaaa aaacgtggtg ttgccatcgc ttacgattca cgtcacttct cgccagaatt
     2521 tgcttttgaa tcagctcaag ttttggcagc gcatggtatc aaatcttatg tatttgaaag
     2581 ccttcgtcca actcctgagt tgtcattcgc tgtgcgccat tacaatgcca tcgcagggat
     2641 tatggtgact gcaagccaca acccgaaaga attcaacggc tacaaggttt acggtgaaga
     2701 cggcggacaa atgccacctg ctgatgcaga tgcattgacc aactttatcc gtgccatcga
     2761 caatccattt gcagttgaat tggctgacct tgaagccagc aaggaaaatg gtttgattac
     2821 ggttcttggc gaagaaactg acgttaaata tcttgaagaa ctcaatgacc tcaacatcaa
     2881 ccctgaattg attgctgaat acggtaaaga catgaagatt gtctatacac cgcttcatgg
     2941 tacgggtgaa atgttggcgc gtcgtgccct tgcacaggct ggttttgaat ctgtccaagt
     3001 tgttgaagca caagcaactg ctgatcctga cttctctact gtcgcttcac caaacccaga
     3061 gagccaagca gcctttgccc ttgcagaaga actcggccgt gaagtcgggg cagatgtcct
     3121 tcttgcaact gaccctgacg ctgaccgtgt tggtgttgaa gttcgtcaag ctgacggttc
     3181 ttactggaac ctgtctggta accaaatcgg cgctatcatt gctaagtaca tccttgaagc
     3241 ccacaagcaa gcaggtacac ttcctgcaaa cgctgccctt gctaagtcaa tcgtatctac
     3301 tgagttggta actaagattg ctgaaagcta tggcgctacc atgttcaacg tcttgactgg
     3361 tttcaaattc atcgctgaga aaatccaaga gttcgaagaa aaacacaacc atacttacat
     3421 gtttggtttc gaagaaagct ttggctattt gattaaacca tttgtacgtg acaaggatgc
     3481 tatccaggct gtgcttatgg ttgctgaaat tgctgcctac taccgttcac gtggcatgac
     3541 cttagctgac ggtatcgatg aaatcttcaa agaatacggc tactttgctg agaaaacaat
     3601 ctctgttacc ctttctggta aggatggtgc agaacaaatc aaggcaatca tggctaagtt
     3661 ccgtgataac tcaccagcac aattcaacgc tactgacatc gcggtctttg aagactttgc
     3721 ccttcaaact aagacagaca aagatggaaa cattgaaaaa ctcactactc ctccatcaga
     3781 tgtcttgaaa tacaccttgg cagatgattc ttggttcgct gttcgtcctt cgggaacaga
     3841 accaaaaatc aaattctaca tcgcaaccgt tggtgaaaca cttgctgaag cagaagaaaa
     3901 aatctccaac atcgaaaaag aaatcaacga atttgttggg taagagtcac ataaattaga
     3961 aagttcaaac cacccgtcaa
//
LOCUS       DBNFCK010000131         3971 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.131, whole genome shotgun sequence.
ACCESSION   DBNFCK010000131 DBNFCK010000000
VERSION     DBNFCK010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.131"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            114..785
                     /locus_tag="AC1PIW_001731"
     CDS             114..785
                     /locus_tag="AC1PIW_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIP6606627.1"
                     /translation="MIKILLVEDDLSLSNSVFDFLDDFADVMQVFDGEEGVYEAETGV
                     YDLILLDLMLPEKDGFQVLKELREKGVTTPVLITTAKESLEDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRAGKFNENTLTFGDVTVDLSTNMTMVNGEEVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRIWGFDSDTTISVVEVYVSKIRKKLKGTTFGENLQTLRSVGY
                     ILKNA"
     gene            778..2157
                     /locus_tag="AC1PIW_001732"
     CDS             778..2157
                     /locus_tag="AC1PIW_001732"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIP6606628.1"
                     /translation="MPKRIGKLVYTDKFSFFIRYFAVFTLIFGLMTAIIFQLMRSTMY
                     QNSDNTLKRIKEEPAMAVGFAIARTYEPNSVFILQDSPTSEETTSSSSDSMPVPKEQK
                     NTRDGDQLRLGVNTHVLLYSKSGEMVNPDTFTGLANLPLDKEKLGEIKETTVESSFGM
                     SEDYRYITIGLATDELGYYSSYDIKYATILVNVSQIKSSIESYESTVAIVMVSAWLIS
                     ILASIYLSNLSMRPILISYQKQKDFVENASHELRTPLAVLQNRLESLFRHPETTILES
                     SESIGSSLEEVRNMRLLTTNLLNLARRDDGLKVDMIDVQPNYFDEIFANYLMIAEENG
                     KTLTVNNLIHQPIRTDKVLIKQLLTILFDNAMKYTDDDGTIQIAANIKDKLVCFTVID
                     NGLGISDVDKKKIFDRFYRVDKARTRQKGGFGLGLSLAQQIIKNLDGEISVRDNQPKG
                     TIFEVRLPK"
     gene            2488..3717
                     /locus_tag="AC1PIW_001733"
     CDS             2488..3717
                     /locus_tag="AC1PIW_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HIP6606629.1"
                     /translation="MRKVSRGVLTILLSATVLSACSGGGQELSERLEAAKIEFSKEKK
                     QLEAELTALKEEVQGNFYYQQLDTDKERRVYLQFVNGLSKRMQVIDIDSVNDEIYSRV
                     YFSVAHDYPEFYWLTDEAAMSGGVDILDLEEPVYPTDLSPTRNKIEEEVNKILAQAPT
                     GSDYEKVKYFYEVIIKQTDYDLEALQTNSVTWRSQGITSVLLDKKSVCAGYSRTFQYL
                     CKKAGIDCIYVTGIAKNGQNGEFGHAWNLVKINGQYYGVDTTWGDPVFDQAISGEAHT
                     DISYDYLCVPDEILERSRIADTDLLDYWGEEQYYEPRALTYPKCTDNSLNYYVQKGVY
                     FTSFDEAAVLQSITDQRLQGTNKVVLQFGTAEAMQQMITLASTENNAIFQALGDVGEY
                     QYYYNDQTYTFELADWF"
BASE COUNT         1284 a          657 c          836 g         1194 t
ORIGIN      
        1 attcattttt cagatggtca tagtagtact ttatctaacc atttctctta aagaccgtga
       61 cctgtcccag gtctttggtt tgtttattaa accgaaacgt aaggaagtgt atgatgatta
      121 aaatattgtt agtggaagat gatttaagtc tatcaaattc tgttttcgat ttcttagatg
      181 attttgcgga tgttatgcag gtttttgatg gtgaagaggg cgtttatgag gcggaaacag
      241 gcgtttatga tttgattctt ttggatttaa tgttacctga aaaagacggc tttcaagtct
      301 tgaaagagtt acgtgaaaag ggggttacga caccagttct gattacaaca gccaaggaaa
      361 gtctagagga taaaggccat ggttttgaat tgggggctga tgactatttg acaaaaccgt
      421 tttatttaga agaattgaag atgcgaattc aagctctatt aaaacgagcg ggtaagttta
      481 atgaaaacac actaaccttt ggagatgtga cagttgacct atctacaaat atgacgatgg
      541 taaatggcga agaagttgaa ctgttaggaa aagaatttga tctactagtt tatttcttgc
      601 aaaatcaaaa tgttattttg ccaaaaacac aaatttttga ccgtatttgg ggctttgaca
      661 gtgatactac aatttcagta gtagaggtat atgtttctaa aattaggaaa aagttgaaag
      721 gaacgacttt tggggaaaat cttcaaaccc tgcgtagtgt cgggtacatt ctaaaaaatg
      781 cctaagcgaa tcggaaaatt agtgtatacg gataaatttt ccttctttat ccgttatttt
      841 gcagtattta ccctgatttt tggcttgatg acagctatca tttttcaatt aatgcgttca
      901 actatgtatc aaaattcaga taataccttg aaacggatta aggaagaacc agcgatggct
      961 gttggttttg cgatcgcaag aacctatgag cccaactctg tctttatact tcaagatagc
     1021 ccaactagtg aagaaacgac gagctctagc tcagatagca tgcctgttcc taaagaacaa
     1081 aaaaatacta gagatggaga tcagctgaga ttaggtgtca atactcatgt cttactctat
     1141 agtaaaagtg gagaaatggt caatccggat acttttactg gtttggccaa cctaccgtta
     1201 gataaggaaa aattgggtga aattaaggaa actacggttg aatcaagttt tggtatgtct
     1261 gaagattatc gctatataac gattgggctg gctacagatg aactaggtta ttattcgtcc
     1321 tatgatataa aatatgcgac aattttggtg aatgtcagcc aaattaaatc ttccattgag
     1381 agctatgaat cgacagttgc tattgttatg gtatcagctt ggttgatttc tatactagca
     1441 agtatttacc tatcgaatct tagcatgcgt ccaatcctaa ttagttatca aaaacaaaaa
     1501 gacttcgttg aaaatgctag tcatgagtta cgcacaccgt tggcagttct tcaaaatcgc
     1561 ctggaaagtc tatttcgtca tcccgagaca actattttgg aaagtagtga aagcatcgga
     1621 tctagtttag aagaagttcg aaatatgcga ctattaacaa cgaacttgct caatttggct
     1681 cgtcgtgatg atggcttaaa agtcgatatg atcgatgtcc aacccaacta ttttgatgaa
     1741 atctttgcta attaccttat gattgccgaa gaaaatggaa aaactctaac agttaataat
     1801 ttgattcatc agccaattcg aacagacaag gttttgatta aacagttgct caccattttg
     1861 tttgacaacg ctatgaagta taccgatgat gatgggacaa ttcaaatagc agctaatatc
     1921 aaggacaaac tagtttgttt tacagttatt gacaatggtt tgggaatcag cgatgtcgat
     1981 aaaaagaaaa tctttgatcg cttttaccga gttgacaagg ctagaacacg tcaaaaaggt
     2041 ggatttggtt taggtttatc tttagctcaa caaattatta agaatctaga tggtgagatt
     2101 tctgtgagag ataaccaacc gaaaggaact atttttgagg tacgtttacc caaataacta
     2161 accattcatc aagattagag gttgggtaaa aaatccagcc tttcttctca aagttcgctc
     2221 tatttccaat ttccaacttc caactgtctc ccaggtagtt ttgtagcgaa ctctgttcgc
     2281 tctatctcca gcctccaaag gtttggggaa ctgtttcagc tggtcaccta aaaataagaa
     2341 agtgaccata gaaaatcgaa cctaaatgtt tggaagtgag gggaattctt tttcgatcta
     2401 ttgaattctt tccactccct ttttgttttg tgtagttcgt cagaagtctc ctgattatgt
     2461 tatacttaaa caagaatgta ggtaaatatg aggaaagttt ctcgtggcgt tttaactata
     2521 ttattatcag cgacagtcct ttctgcttgt tcaggaggag gacaagagct gagtgaacgt
     2581 ttggaagcag ctaaaattga attttctaaa gaaaaaaagc aactagaagc agaattaaca
     2641 gcattaaaag aagaggttca aggaaatttc tactaccaac aattggatac tgataaggaa
     2701 cgtcgtgtct atctccaatt tgttaatggg ctcagcaagc ggatgcaagt gatcgatatt
     2761 gattctgtta atgatgaaat atattcgcgc gtctattttt ctgtcgctca tgattatcca
     2821 gaattttact ggctaactga tgaagcagca atgagtggcg gagttgatat attggattta
     2881 gaagagccag tgtatccgac tgatttatct ccgacacgaa ataaaataga agaggaagtg
     2941 aataagatac ttgctcaggc accgacaggg tcggactatg agaaggttaa atatttttac
     3001 gaggtgatta taaagcaaac ggattacgac cttgaagccc tacaaaccaa ctctgtcacg
     3061 tggagaagcc aaggaattac cagtgtgcta ttagataaga aatccgtttg tgctggctat
     3121 agtcgcacct tccaatatct atgtaaaaaa gcaggcattg attgtatcta tgtcacaggg
     3181 attgcaaaaa atgggcaaaa cggtgagttt gggcatgctt ggaatctagt caagataaat
     3241 ggacaatact atggtgtcga tacgacttgg ggagatccag tttttgatca agctataagt
     3301 ggagaagcgc atacagatat ttcatacgat tatctatgtg tccctgatga aatattagag
     3361 agaagtagaa ttgctgatac cgacctactc gactactggg gagaagaaca atattacgaa
     3421 cctagagcat tgacctatcc aaagtgtaca gataattcat tgaattacta tgttcaaaag
     3481 ggagtttatt tcacaagctt tgatgaagct gcagttttac agtccatcac tgaccagcgg
     3541 ctacaaggta cgaataaagt agtcttgcaa ttcggaacag cagaagcaat gcaacaaatg
     3601 attacattgg cctctactga aaataatgct atttttcagg ctttgggcga tgtaggagag
     3661 tatcagtact attataatga ccaaacctat acatttgaac tggcagattg gttttaagga
     3721 attagtttga atctccagga gcgtgttctt ccctaataat aaaaaatagg gagtgggaaa
     3781 gacatccatg atggctccgc ctcacccaca gaataatgaa aacgaacata aagccctcac
     3841 taggtgtata atcttagtga gggctttacg gttgacgagt taatgatgtt actcatcgag
     3901 ggaaaactga tccactttgt cccgcctcct tatgatggaa aaaataaaaa aaaaggaaac
     3961 ccaaaggttt c
//
LOCUS       DBNFCK010000132         3968 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.132, whole genome shotgun sequence.
ACCESSION   DBNFCK010000132 DBNFCK010000000
VERSION     DBNFCK010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.132"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            398..1204
                     /locus_tag="AC1PIW_001734"
     CDS             398..1204
                     /locus_tag="AC1PIW_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HIP6606630.1"
                     /translation="MQPTYNIDNPNLSYEAKQELWETGFGLQKVDGLTPSVYMEELAE
                     RQARGEYTYEQVYEEITKYHQSTDASTQEADIVSLRIVEMLSQNGFSLRPTTLLHIHK
                     ELFHDIFDSSIPVVEYRTVNITKNELVLKGDTVIYSDFPLIAATLDYDFQQERDFSYA
                     RLDKKAIVAHIQSFISGVWQIHPFREGNTRTITVFLIKYLRSLGFEIDNEPFQKHAKY
                     FRDALVLDNAKLVNKRSDFLTAFFENLLLNGQNDLSSERMYEELGLDEYQ"
     gene            1299..>3968
                     /locus_tag="AC1PIW_001735"
     CDS             1299..>3968
                     /locus_tag="AC1PIW_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOTE conflict system archaeo-eukaryotic primase
                     domain-containing protein"
                     /protein_id="HIP6606631.1"
                     /translation="MSYYYEKLTGKVAKHLARFPYYATDKILNLMQFQDNNQQFLISD
                     KHLYDYFEEQKHQLSTDEKLSILFNLFKGRIDVYAKSYIDENGKINYFPSYNYGWKKR
                     PVEKRTCQPLTKQVLLAHLRGEISIGIFPMSLSDTCSFLAIDFDKNNWREEVSILRDT
                     AEQHGFEGHIEISRSGNGAHLWFFFEEEIACQQARNVGKRLLELAMQESKDIRFSSFD
                     RMFPNQDILPKGGFGNLIALPLQGEAFKKGRTVFVDKHFQPFSEQWTYLQQVQKIGQK
                     KILDFLGQEFSDTVDDTVLDCSLSNVIRVEKRAISSKTNYLLRKLASFPNPEFYLKQA
                     TRQPTYQTPERIYLFEETNEALHLPRGILAKLQEIFETVTIRDNRNSLSPIQISFKGR
                     LRFEQELALADLLASENGLLCAETGFGKTVLGAALIAQRKCRTIILVHNRQLLEQWLE
                     RLGEFLEIEEEEAVRYTPSGRVKVIGHIGQYGASKKWRSKLVDVVMIQSLFQLDVISD
                     FLSDYDMMIVDECHHVTALQFEKVVAQFASQYLYGLTATPECKNGHQPIVFQRIGPIL
                     HTAQSGQYDFKKRLLLHLTSFGKLDLEQSNSTNFASLNDWLAKDLHRNTLIVQDIFKL
                     YQEKRNILVLVNRREHIELLEKLLIEKEMPNIFCLSGASKRRDTKELLKRISELDENS
                     PFVLISTGKFIGEGFDMPKLDTLILAAPLSWKNNLIQYAGRLHRPYQGKTEVRIVDYL
                     DIHVPYLERMYQKRQIAYRKMAYQVGEKEQTQVLFSGRDYEEKFREDLRNTRSTVYLQ
                     LHSFTSSRIQELLGLLRGKQVVIHVSKNHKLSEWLMGLNSDNVKVKLVPERIGTTAVI
                     LDSNLVWYGNLSPFTYQSDDQASLLRLESQSIAT"
BASE COUNT         1286 a          679 c          830 g         1173 t
ORIGIN      
        1 aaaacagttg aagagtatgt ggagttgatt gctgaaagag tttctggaat tgataatctt
       61 tcaattctac agaaaggaca aattatttcc aaattgaatt atattttatt tggtcaagat
      121 aataatatta aaataagaca aaaagaaaat atcgctataa ataaatttta agagctccat
      181 tggacataga aaaacttgag attttatcaa gcgttaccaa tctggaaatc gtctaaaaat
      241 aaaaacaagc tgactaccaa gcaaaatatg atgcgacaca ggagcggatt gaagagatga
      301 aaaaactggt taagtaggta gaatacaaca aatgcccagc ctaaaaaggc tgggttttgt
      361 ggtataataa ggttagaaat tcaaacagga ggagtctatg caaccaactt acaatattga
      421 taatcccaat ctttcctacg aagccaagca agaactctgg gaaacgggtt ttggtcttca
      481 aaaagtggat gggttgactc cgtctgttta tatggaggag ttggcggaaa ggcaagctcg
      541 tggagagtac acctatgagc aggtgtacga ggagattacg aaataccacc agtcaactga
      601 cgccagtacg caagaggcag acattgtctc tctccgaatt gttgaaatgc tctcccaaaa
      661 tggttttagc ctccgaccaa ccactctctt acatatccac aaagagctct ttcacgatat
      721 ttttgacagc agtattccag tagttgaata ccgcactgtc aatattacaa aaaatgaatt
      781 agtcttaaaa ggtgatacag tgatttattc agattttccc ttgattgccg ccactttgga
      841 ctatgatttc cagcaagaaa gggatttttc ttatgctagg ttagataaaa aagccatcgt
      901 cgctcatatc caatccttta tttcaggtgt ttggcaaatc cacccattcc gtgaagggaa
      961 tactcgaaca atcaccgtat ttctcatcaa ataccttcgc tcacttggtt ttgagattga
     1021 taatgagcct tttcaaaaac atgcaaaata tttccgtgat gcgcttgtac ttgacaatgc
     1081 taagttagtc aataaacgct cagatttttt aactgccttt tttgaaaatc tcctcttgaa
     1141 cggtcagaat gatttatcaa gtgagaggat gtacgaggag ttggggttag atgaatatca
     1201 ataaagacag gatagttttc aagtcggaaa tagaccagtg aatacttggg cttttgactt
     1261 ttcgttgata atctcgttag gcaatttgga gaaatcgtat gtcctattat tatgaaaaat
     1321 taacgggtaa ggttgcaaag catcttgctc gttttcccta ttatgctacc gataagattc
     1381 tgaatttaat gcaatttcaa gataacaatc agcaatttct gataagtgat aagcatttgt
     1441 atgattactt tgaagaacaa aaacatcaac taagtaccga tgaaaaactc tccattctgt
     1501 tcaacctatt taagggacgt atagatgttt atgcgaaatc ttatattgat gaaaatggaa
     1561 aaatcaacta ttttccttct tacaactatg gttggaaaaa acgtcctgtt gaaaagagaa
     1621 cttgtcaacc attgaccaaa caggtattat tggctcacct acgtggcgaa atcagtattg
     1681 gtatttttcc aatgagttta tcagacacgt gttccttttt ggcgattgat tttgataaaa
     1741 ataattggag agaagaggtc tccattctaa gagatacagc agaacaacat ggttttgagg
     1801 ggcatatcga aatctctcgt tcgggcaatg gtgctcatct ctggtttttc ttcgaggaag
     1861 aaattgcttg tcagcaggcg agaaatgttg gaaaaagact gctggaattg gctatgcagg
     1921 agtcaaaaga tattcgcttt tcttcatttg accgtatgtt tccaaatcaa gatattctgc
     1981 ccaagggagg atttggcaat cttatcgcac tgcccttaca aggggaagct ttcaagaagg
     2041 gaagaaccgt ttttgttgat aaacattttc agcccttttc tgaacaatgg acctatcttc
     2101 aacaggttca aaagattgga cagaagaaaa ttctagattt cttaggacag gagttttcgg
     2161 atactgtaga tgatacagta ttagattgta gcttatcaaa cgttattcga gttgagaaga
     2221 gggctatttc ttcaaaaaca aattacttat tgagaaagct ggcatctttt cctaaccctg
     2281 aattttatct aaaacaagcg accagacaac caacctatca gactccagaa cgcatctatt
     2341 tatttgaaga aacaaacgaa gccttgcatc ttccaagagg aatacttgct aagctacagg
     2401 aaatctttga aacggtaact attagggaca atcgcaacag tttatctccg attcagattt
     2461 ctttcaaagg gaggttgcgt tttgagcagg aacttgcttt ggcagattta ttagctagtg
     2521 agaatggctt gctatgtgct gaaacaggtt tcgggaagac agttttaggg gcagccttga
     2581 ttgcccagag gaaatgtaga acgattatct tagttcacaa tcgtcaactg ttggagcagt
     2641 ggttggaaag gttgggtgaa tttttagaga ttgaagagga agaggcagtt cgctatactc
     2701 cttcagggcg tgtaaaagta atagggcata ttgggcaata tggggcttct aaaaaatgga
     2761 ggagtaaatt ggtggatgtg gttatgattc aatccctctt ccaattggac gtgatttccg
     2821 actttttgtc ggactacgat atgatgattg tagatgaatg ccatcatgtg acagccttgc
     2881 agtttgaaaa agtagttgct caatttgcta gtcaatatct atatggccta acagcaacgc
     2941 cagagtgtaa aaatggacat caaccgattg tctttcagcg tattggtcca attttacata
     3001 ctgctcaatc aggtcaatac gattttaaaa agaggttact actccatcta acatcttttg
     3061 ggaaattgga cttggaacag tcgaatagta cgaactttgc atcccttaat gattggcttg
     3121 ccaaagattt acatcgtaat actttgatcg tgcaagatat tttcaaacta tatcaagaaa
     3181 aacgaaacat ccttgtctta gtcaatcgac gggaacatat agaattattg gaaaagctac
     3241 ttattgaaaa agaaatgccc aatatttttt gtctgagtgg agcgagtaaa agaagagata
     3301 cgaaagaact cctgaaaaga atcagtgagc tagatgagaa tagtccattt gttctcattt
     3361 caacaggaaa atttattgga gagggcttcg atatgccaaa gttggataca ttaattttgg
     3421 ccgctccttt gtcttggaaa aataatctca tacagtatgc aggacggctt caccgtccct
     3481 atcaaggcaa aactgaggtg agaatagttg actatcttga tatacacgtt ccctatcttg
     3541 aaagaatgta ccagaaacgt cagatagcct atcgtaaaat ggcctatcag gttggagaga
     3601 aagaacaaac ccaagtatta ttcagtggta gggactacga ggagaaattt agagaggatt
     3661 tgcggaacac acgttcaaca gtctatttac aattgcattc gttcacttct agtaggattc
     3721 aagaattatt gggactgctc cgaggaaaac aagtagttat tcatgtttcc aaaaatcata
     3781 agttaagtga gtggctaatg gggttaaaca gtgacaatgt aaaggtgaag cttgtaccag
     3841 aaaggattgg tacgaccgca gttatcttag atagtaatct cgtttggtat ggcaatttat
     3901 cccccttcac atatcagtcg gatgaccaag ctagtttgtt gagattggaa agtcaatcta
     3961 ttgcaact
//
LOCUS       DBNFCK010000133         3950 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.133, whole genome shotgun sequence.
ACCESSION   DBNFCK010000133 DBNFCK010000000
VERSION     DBNFCK010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.133"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..559)
                     /locus_tag="AC1PIW_001736"
     CDS             complement(<1..559)
                     /locus_tag="AC1PIW_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HIP6606632.1"
                     /translation="MALKDTFKNLFNYFEVDEVNEVEEQADAYSMPNDRPKMRVANTT
                     VAPVREQQPKVETRREARSESQLQRLHERQQELMTNNNEKEIVKTTIDIKFPKRYEDA
                     PEMVNLLLDNASILIDFQYMSEQQARRCLDYLDGARSVLSGNLKKVSNTMWLLTPVNV
                     TVNIEELRNAGTTTGVADSNFEFDIK"
     gene            complement(576..1247)
                     /locus_tag="AC1PIW_001737"
     CDS             complement(576..1247)
                     /locus_tag="AC1PIW_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HIP6606633.1"
                     /translation="MNFQKNKDAIFSAVAKAAEKAGRPSESVKVIAVTKYVDSSIANQ
                     LVNTGITHIGENRVDQFLEKYNALADKQLTWHLIGTLQRRKVKDIIPFVDYFHALDSV
                     KLAQEIDKRATKIVKCFLQVNVSGEESKHGFAVSEIDAALAEISLLENIELVGLMTMA
                     PIDATDDELDEIFSKMQEIQEELATRNLPRMPFTELSMGMSGDFERAIAHGATYVRIG
                     SAFFE"
     gene            complement(1254..2483)
                     /gene="ftsZ"
                     /locus_tag="AC1PIW_001738"
     CDS             complement(1254..2483)
                     /gene="ftsZ"
                     /locus_tag="AC1PIW_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIP6606634.1"
                     /translation="MAFSFEAAASHGAVIKVIGVGGGGGNAINRMIEEGVAGVEFIAA
                     NTDVQALSSSKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEEALTNVLTGADMVFI
                     TAGMGGGSGTGAAPVIARIAKNLGALTVAVVTRPFGFEGNKRGNFAIEGIEGLREQVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MENKGNALMGIGIGTGEDRVIEAARKAIYSPLLETTIDGAEDVIVNVTGGYDMTLTEA
                     EDASEIVNQAAGQGVNIWLGTSIDETMKDEIRVTVVATGVRQDTADKPARHRTEAVSP
                     RPSHRFDHSVASAPTRGAAQQTEAPKASAFGEWDLRRENLIRPTDTEPTSTVSVEKFT
                     MDQDEDELDTPPFFRNR"
     gene            complement(2509..3888)
                     /gene="ftsA"
                     /locus_tag="AC1PIW_001739"
     CDS             complement(2509..3888)
                     /gene="ftsA"
                     /locus_tag="AC1PIW_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIP6606635.1"
                     /translation="MARNGFFTGLDIGTSSVKVLVAEYIDNEMNVIGVSNVKSAGVKD
                     GIIVNIEVAAGAIKKAIEQAEEKSGIRIEKVNVGLPANLLQIEPTQGMIPVTTDSQEI
                     TDLDVENVVKSALTKSMTPEREVISFIPEEFTVDGFQGIKDPRGMMGIRLEMRGMLYT
                     GPRTILHNLRKTVERAGVQVENIVISPLALTRSVLNEGEREFGATVIDLGGGQTTVAV
                     MRGQELQYTNIYQEGGDYITNDISKVLTTSKSIAENLKYNYGIAYPQDASDKEKFTVE
                     VIGENAPVEVTERYLSEVIAARLRQIFDRVKQDLERTRALDLPGGIVLVGGGAILPGI
                     IELAQEVFGVNTKLFVPNQIGIRNPAFASVISFVEYVGELEDVENIAQHAVNGETTLR
                     HKPVEIPVARPRISQPIQREIVNELNTVDQVEEQVAPTYHDEEEEVRPAAKGNITDRI
                     RGLFGSMFE"
BASE COUNT         1086 a          955 c          683 g         1226 t
ORIGIN      
        1 gttttatatc aaattcaaaa tttgaatcag ccacaccagt agttgtacca gcattgcgca
       61 attcttcaat attgactgtg acatttactg gagttaacag ccacatggta tttgagacct
      121 ttttcaaatt tccagataat actgaacgag caccatccaa gtagtctagg caacgtcttg
      181 cttgttgctc cgacatatat tgaaaatcaa tgagaatact cgcattgtct aataaaagat
      241 ttaccatctc tggtgcatct tcataacgtt ttggaaactt aatatcaatt gtagttttga
      301 caatctcttt ttcgttatta ttagtcatca attcttgctg cctttcatgt aggcgttgaa
      361 gttgactttc tgaacgtgct tcccgacgcg tctctacttt tggctgctgt tcgcgcacag
      421 gtgcaactgt agtattggca acacgcattt taggacgatc attaggcatt gaataggcat
      481 ctgcctgttc ttcaacttca ttgacttcgt caacttcaaa gtaattaaat aagttcttaa
      541 atgtatcttt taatgccata ttttcctcct ctccattatt caaagaatgc tgagccaatt
      601 ctcacatatg tagctccgtg agcaatggcg cgttcaaagt ccccactcat tcccatactt
      661 aactctgtaa agggcatacg tggtaagttc cttgtagcta gttcttcttg gatttcctgc
      721 attttagaaa agatctcgtc taattcatca tctgtagcat caatcggtgc catcgtcatc
      781 aatccaacaa gttcaatgtt ctccaataaa ctaatttcag caagtgccgc atcaatctca
      841 gatactgcaa agccatgctt actctcctca ccggaaacgt tgacttggag gaaacacttg
      901 actattttag tcgcacgttt atctatttcc tgagctagtt tgacagagtc tagggcgtgg
      961 aaataatcaa caaatggaat aatgtccttg acctttcttc tctgtaaagt ccctatcaaa
     1021 tgccatgtca attgtttgtc tgctagggca ttatactttt ctaagaactg atcgactcga
     1081 ttttctccaa tatgagtaat tccagtattc actagctgat tagcaataga gctgtcaaca
     1141 tatttggtga cagctattac ctttactgat tcacttggac gacctgcctt ctcagcagct
     1201 tttgcaactg ctgaaaagat agcatcctta ttcttttgaa aattcatttt agtttagcga
     1261 ttacggaaga atggtggtgt atccaattca tcctcatctt gatccattgt aaatttttcg
     1321 actgaaacag tggatgttgg ttctgtgtca gttggacgaa tcaagttttc acgacgcaaa
     1381 tcccactctc cgaaggcaga agctttcggc gcttcagttt gttgtgcagc tccacgagtt
     1441 ggtgctgatg ctactgaatg atcaaagcgg tgagaagggc gtggtgaaac agcttccgta
     1501 cgatggcggg ctggtttatc agcagtatct tgacgaacgc ccgtagccac aacggtcaca
     1561 cgaatctcat ctttcattgt ttcatcaata gatgtaccca accaaatgtt aacaccttga
     1621 cccgcagcct gattaacgat ttctgaagca tcttctgctt ctgtcagagt catgtcatag
     1681 ccacctgtaa cattgacaat gacatcctct gcaccatcaa ttgttgtttc aagaagagga
     1741 gaataaatcg ctttacgagc cgcctcaatc actcggtcct caccagtacc gataccgata
     1801 cccatcaagg cgtttccttt gttttccata actgtcttca catctgcaaa gtccaagtta
     1861 atcaaaccag gatttgtaat caagtctgta attccttgaa caccttgacg aagaacatta
     1921 tcagcttcgc ttagtgcttc taaaagcggt gttttcttat caacaatttc aagaaggttg
     1981 ttgtttgaaa taattaaaag tgtatctact tgttcgcgaa gaccttcaat tccctcaatc
     2041 gcaaagtttc cacgtttatt tccttcaaaa ccaaacggac gagtcacaac tgcgacagtc
     2101 aacgcaccca agtttttagc aatacgtgca ataacaggag ccgcaccagt ccctgagcca
     2161 ccgcccatac cagcagtgat gaagaccata tctgcaccag ttaatacatt agtcaatgct
     2221 tcttcgcttt cctcagcagc tttacgacca acctcaggtt gacctccagc acccaaacca
     2281 cgagtcaatt taggacctag ttggataact gtttcagctt tagagctact tagcgcttgt
     2341 acatctgtgt ttgctgcaat aaactcaacc ccagcaacac cttcttcaat catacggttg
     2401 attgcgttcc cgccaccacc gccgacaccg attactttaa tgacagcacc atgactagct
     2461 gctgcttcaa atgaaaatgc catagtttaa atcacctttt cttttctctt attcaaacat
     2521 gctgccaaat aaaccacgaa tgcgatctgt gatgtttccc ttagcagctg gacgaacttc
     2581 ttcttcctca tcatggtaag taggagcaac ttgttcctct acctgatcaa ctgtgttcaa
     2641 ttcgttcacg atttctcttt gaattggctg actaatccga ggacgagcaa caggaatttc
     2701 aactggctta tgtctcaagg tagtttcacc atttactgca tgttgggcaa tattttctac
     2761 atcttctaac tcaccaacat actcaacaaa gctaatcaca cttgcaaatg ctggattgcg
     2821 gataccaatt tgatttggaa caaaaagctt agtgttaaca ccaaacactt cttgggccaa
     2881 ttctataatt cctggtaaga tagcaccacc acctacaaga acaatgcctc ctggtaaatc
     2941 tagagcacgt gtacgctcca aatcttgttt cacacggtca aaaatctgac gcagacgtgc
     3001 cgcaatcact tctgaaaggt agcgctcggt cacttcaact ggagcgtttt ctccaatcac
     3061 ttcgacagta aatttttcct tatcacttgc atcttgcgga taggcaatgc cgtagttgta
     3121 tttcaagttt tcagcaatac tctttgaagt tgtcaatact tttgaaatat cgtttgtgat
     3181 ataatctcca ccttcttggt agatgtttgt atattgcaac tcttggccac gcataacagc
     3241 cacagtcgtt tgaccgccac caagatcaat gacagtcgca ccaaactcac gttcaccttc
     3301 attcaataca gagcgagtta gtgccaacgg agaaattaca atattttcaa cttggacacc
     3361 tgcacgctcc accgttttac gaagattgtg aagaatggta cgtgggcctg tatacagcat
     3421 accacgcatt tccaagcgaa ttcccatcat tccacgaggg tccttaatgc cttggaatcc
     3481 atcgacagtg aactcttctg gaataaatga aatcacttcg cgttccggag tcatactctt
     3541 tgtcaatgct gattttacaa cattctcaac atccaagtct gtaatttctt gtgaatctgt
     3601 tgtaacagga atcatccctt gagtcggttc aatctgaaga aggttggcag gtaatccaac
     3661 atttaccttt tcaatgcgga tacctgactt ttcttctgcc tgctcgatcg cttttttaat
     3721 cgctccagca gccacctcaa tatttacaat aattccatct ttgacgccag cactttttac
     3781 attgctgacc ccaatcacat tcatttcgtt atcaatatat tctgcaacca agactttaac
     3841 ggagcttgta ccaatatcta atcccgtaaa aaagccgttt ctagccattc tatcagtcct
     3901 ctcttacttc caactttaac aaatcatctt ccattttgaa aaatagtata
//
LOCUS       DBNFCK010000134         3909 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.134, whole genome shotgun sequence.
ACCESSION   DBNFCK010000134 DBNFCK010000000
VERSION     DBNFCK010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.134"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(359..1294)
                     /locus_tag="AC1PIW_001740"
     CDS             complement(359..1294)
                     /locus_tag="AC1PIW_001740"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HIP6606636.1"
                     /translation="MSKFLVFGHQNPDTDAIASSYGWAHLEREVFGRDAEAVVLGTPN
                     EETAFALDYFGVTAPRVVESAKAEGVSQVILTDHNEFQQSIADIKEVEVAAVIDHHRV
                     ANFETANPLYMRLEPVGSASSIVYRAFKENGVIPPKEVAGLLLSGLISDTLLLKSPTT
                     HVTDPQVAAELAEIAGVNLEEYGLALLKAGTNLASKSAEELIDIDAKTFGLNGNDVRV
                     AQVNTVDIAEVLERQAEIEAAMTAASVANGYSDFVLMITDIVNSNSEILALGSNMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTDAFNA"
     gene            1433..3130
                     /locus_tag="AC1PIW_001741"
     CDS             1433..3130
                     /locus_tag="AC1PIW_001741"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HIP6606637.1"
                     /translation="MKFSDYTYVRPDYEAIKAQFTDLTDQLAQASDLETTRTLVAAVT
                     KLLNLVDTQYNLWMIRHTIDMNDEFYNEETKFWNEYSPLFEELTTNYYRIIVQTPYKE
                     ELSDILPQTFFMQAENKLKTFSSDVIPLLQKENELTDEYSKLIAGAEIDFQGQTYNLS
                     QMGPFGQSTDREVRKAASAATTTFFESKEADFDRVYDELVKVRTEIAHKLGFKDYVEY
                     GYLKMNRFDYNRDMVKVYREEILKHIVPIVQNLRQRQANRLQVPSLKHYDLNLEFLDG
                     NAVPQGDPDFIVSQAQDMYRELSAETGEFFDFMIEHELLDLVAKPGKNSGGYCTYIPD
                     FKSPFIFSNFNGTSGDIDVLTHEAGHAFQVYRSRWIQSPEVVWPTYETCEIHSMSMEF
                     MTWPWMDRFFKEQVDKYKFTHLASALLFLPYGVLVDHFQHEVYERPEMTPAERKATWK
                     KLQDLYLPDRDYSESEALNRGIFWYRQGHIFASPFYYIDYTLAQVCALQFWKRTQVDH
                     DENAWEDYIRICDLGGTKSFLQVVEAANLQSPFKEGALESTAKAAADWLNTVKDSDL"
     gene            complement(3240..>3909)
                     /gene="pflA"
                     /locus_tag="AC1PIW_001742"
     CDS             complement(3240..>3909)
                     /gene="pflA"
                     /locus_tag="AC1PIW_001742"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HIP6606638.1"
                     /translation="QYCHNPDTWDLVNPAATERTAEDVLNEALRFRMFWGKEGGITVS
                     GGEATIQIDFLIALFTLAKEKGIHTTLDTCALTFRKTERYLEKYSRLMEVTDLVLLDI
                     KEMNPEQHRIVTGHSNKTILECAQYLSDIGKPVWIRHVLVPNLTDRDEDLIELGKFVK
                     TLKNVERFEVLPYHNLGEFKWRELGRPYPLEGTKPPTKARVENAKALMETETYQEYLN
                     RIKG"
BASE COUNT         1124 a          919 c          778 g         1088 t
ORIGIN      
        1 atttctaagt tatggcacat tcaaagtaaa gaacaagcgc ctgaattttt ctatgatgca
       61 gatcattgtt tttatctcgg agtgctcgct ttaattcgtc aacttatact caatgaaaat
      121 caaactaggc gatgcatatg cagatagaac tgaagtttat caaatcaagt caacaatgtc
      181 tgattttgat tttcgaagag tattaaatag gagtgggttt atttgtcatg atgccacttg
      241 tttattttgt taactatcac ttcataaaac atgattgtcc tcacaataaa aaaagcaccc
      301 gaaggtgcta ggtaagatca tcctaccgac ttcacgttga ggtaggtaga aaaattcatt
      361 aggcgttgaa agcgtcagtc aactgtggca ccacttgttt cttacgtgaa acggcacctg
      421 caaggaaggc gtggttgttt tccaacttga agttgaaggc tgcttctacc ttgtccatgt
      481 tgctaccgag cgcaaggatt tctgagtttg agttaacgat gtctgtaatc atcaaaacaa
      541 agtcagagta gccatttgct actgatgctg ctgtcatagc tgcttcgatt tctgcttggc
      601 gttccaagac ttccgcaatg tccactgtgt taacttgggc tacacgcacg tcattgccat
      661 tcaagccaaa tgttttggca tcgatgtcaa tcaattcttc tgctgacttg ctggcaaggt
      721 tggtaccagc tttcaagagt gccaaaccgt attcttctaa gttgacacca gcaatttcag
      781 ccaattcagc tgctacctgt ggatctgtta catgagtagt tggcgattta agcaagaggg
      841 tatctgaaat caaacctgat aggagaagtc cagctacttc ttttggaggt attactccgt
      901 tttctttaaa ggcacggtaa acgattgatg atgctgaacc tactggctcc aaacgcatgt
      961 acaatggatt tgccgtttca aagtttgcga cacggtggtg gtcaatgaca gctgccactt
     1021 ccacttcctt gatgtctgcg atagactgtt ggaattcatt gtggtcagtc aagatgactt
     1081 ggctaacacc ttctgctttt gcagactcaa ccacgcgcgg tgcagtaaca ccaaaatagt
     1141 caagagcaaa agcagtttct tcatttggcg ttcctagaac tactgcttcc gcatcacgac
     1201 caaacacttc acgctccaag tgagcccagc cgtatgatga tgcaatggca tctgtatcag
     1261 gattttggtg accaaaaact aaaaatttag acatgtctac aacctcattt tttctaataa
     1321 tctcctacat tttaccataa atagaaagac ttgtgaacat ggacgggtcg ggatttgcca
     1381 aacgttttca aaaagagtaa gatagaaggt agcaatacaa aaggagaaac atatgaaatt
     1441 ttcagactat acctatgtcc gtccagatta tgaagctatc aaggctcaat ttactgacct
     1501 gaccgaccag ttggctcagg caagtgactt ggaaacaact cgcacacttg tggcagccgt
     1561 taccaaatta ctcaacctgg tcgataccca gtacaatctc tggatgatcc gtcatactat
     1621 tgacatgaac gatgagttct acaatgaaga aaccaagttt tggaatgaat attcgccact
     1681 ttttgaagaa ttgaccacca actactaccg tatcattgtc cagacgccgt acaaggaaga
     1741 actgtcagac atcttgcctc aaaccttctt catgcaggca gaaaacaagc tcaagacctt
     1801 ctcatcagac gtcattccat tattgcaaaa agaaaatgaa ttgaccgatg agtacagcaa
     1861 gctgattgct ggtgcagaga ttgatttcca aggtcagacc tacaacttgt cacaaatggg
     1921 gccattcggt caatcaaccg accgtgaagt ccgcaaggct gcttctgctg ccacaactac
     1981 cttctttgaa agcaaggaag ctgattttga ccgtgtttac gatgaattag taaaagtccg
     2041 cacagaaatc gcccacaaac ttggcttcaa ggactatgtg gaatacggct acctcaagat
     2101 gaaccgtttc gattacaatc gtgacatggt taaggtttac cgtgaagaaa tcctcaagca
     2161 tatcgtaccg attgtacaaa atctgcgtca acgccaagcc aatcgcttgc aggtgccaag
     2221 cctcaaacac tacgacctaa accttgagtt cttggatgga aatgctgttc ctcaaggtga
     2281 ccccgacttt atcgtcagcc aagcccaaga catgtaccgc gaattgtccg cagaaacagg
     2341 cgagttcttc gatttcatga ttgagcatga actcttggac ttggtcgcaa aaccaggcaa
     2401 aaacagcggt ggctactgta cctatatccc tgacttcaag agcccattca tcttctccaa
     2461 cttcaacgga accagcggtg atattgatgt cttgacccac gaagcaggtc acgccttcca
     2521 agtctatcgt tcgcgttgga ttcaaagtcc agaagttgtt tggccaacct acgaaacctg
     2581 tgaaatccac tccatgtcta tggaatttat gacctggcca tggatggacc gtttcttcaa
     2641 ggaacaagtg gataaatata aatttactca cttggcaagt gcccttctct tcttgcctta
     2701 cggtgtcttg gtagaccact tccagcacga agtctatgaa cgcccagaga tgacaccagc
     2761 agaacgcaag gcaacttgga aaaaactcca agacctctac ttgccagacc gtgactattc
     2821 cgagtcagaa gccctcaacc gcggtatctt ctggtatcgt caaggtcata tctttgctag
     2881 tccattctac tacatcgact ataccttggc ccaagtttgt gccctccaat tctggaaacg
     2941 tacacaggta gatcacgatg aaaatgcttg ggaagactac atccgcatct gtgatttggg
     3001 cggcaccaag tccttcttgc aagtggttga agccgcaaac ctccaatcac cgttcaaaga
     3061 aggtgccctt gaaagcacag ctaaagctgc tgctgattgg ttaaatacag ttaaggatag
     3121 cgacttataa aacacaaaaa tctgaatgaa tcaaatgact cattcagatt ttttgattat
     3181 atgatacttg tccctaacac attaaatctt tttcgcatca atctccgacc tcttcactat
     3241 tatcccttaa ttctattcaa gtactcctga taggtctctg tctccatcaa ggcctttgca
     3301 ttttccactc gagccttcgt tgggggcttg gtcccctcta gtggataggg tctgcccaac
     3361 tcacgccact taaattcacc aagattgtga taaggtagca cctcaaaacg ttcgacattt
     3421 tttaaggttt taacaaactt accaagttca atcaagtcct catctcggtc agtcaaattt
     3481 ggtaccagga catgacgaat ccaaacaggc ttaccaatgt ctgatagata ttgagcacat
     3541 tctaaaatag tcttgttact gtgacctgtc acaatgcggt gttgctcagg attcatttcc
     3601 ttaatatcaa ggagcaccaa atctgttact tccataagcc gactatattt ttccaagtaa
     3661 cgctctgtct tacggaaagt caacgcacag gtatccaaag tagtgtgaat ccctttttct
     3721 tttgcaaggg taaacagagc aattaaaaaa tcaatctgga tggttgcttc accacctgaa
     3781 acggtaatcc ccccttcttt tccccaaaac atacgaaaac gcagagcttc attcaagaca
     3841 tcttccgccg ttctctcagt cgctgctgga ttgaccagat cccaagtatc tgggttatgg
     3901 caatactgg
//
LOCUS       DBNFCK010000135         3789 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.135, whole genome shotgun sequence.
ACCESSION   DBNFCK010000135 DBNFCK010000000
VERSION     DBNFCK010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.135"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            52..840
                     /locus_tag="AC1PIW_001743"
     CDS             52..840
                     /locus_tag="AC1PIW_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606639.1"
                     /translation="MAVLKYSKVLLLVLLIATGLSCIGIYWLGKEQNRLLNEQCHALN
                     IRIINDLGTKIDAIGGPQNPRIIGFFQQDDTTAISQRIGTASEEELKIAKPDNLFQKE
                     WIVLYPQTRSSPFENASAYAVMKTSIKADWLHVTTSSETELDIFYEKADESLLTLEDL
                     VQDKESFRATLKTILVSAKNEAEIQVQKDILEMFESDDWSAIPFAYTEKSLILEKAVI
                     SISAFVDSLNPYYFSEQTLADLRLSEESRQALEDSVDKTIITYP"
     gene            868..1815
                     /locus_tag="AC1PIW_001744"
     CDS             868..1815
                     /locus_tag="AC1PIW_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606640.1"
                     /translation="MDVFPSFFISMFNELNIFVDLKNIFCYTCVMKTSTLGKLLELPN
                     FHHFYTVPIRENTESSGNYIIVFEQDKQPVFVDLKNETGHTREVRFASHFDSAGLLVG
                     LGAIPINYWSTCQILGVKKNNNRRGLSLSSLMDGYRRLPIFKEMAERYRARGQSTIYD
                     PYRYSYTIDEKKVIKDNQLTLIPKKEYEKIKDFHPFLQTKHGNVYAVPCFYQDRKRNQ
                     ITKIYIMGWYFYEKQLKISWKPSHSRKSRWRSSLVSVDKLSDRFFFQGELYKKGTYPV
                     FLEDLTIKNKAYQHRLDREFAAFLSIIEEQKQRAQLRVK"
     gene            complement(1836..2189)
                     /locus_tag="AC1PIW_001745"
     CDS             complement(1836..2189)
                     /locus_tag="AC1PIW_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HIP6606641.1"
                     /translation="MTFTSFEPTRNVQDFHLAAFAYYDGLDVIDQLKPGTPVQLVGEP
                     SNPHDSEAVAIFYQGTKLGYIPSDKNSLISRLIYFGHGDILEARIQMSNTENHPDRQF
                     RVVVKLKDNRKNDSR"
     gene            complement(2211..2960)
                     /locus_tag="AC1PIW_001746"
     CDS             complement(2211..2960)
                     /locus_tag="AC1PIW_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6287 domain-containing protein"
                     /protein_id="HIP6606642.1"
                     /translation="MKRKYLALALGLLTIMSLSACQTEKKGSSASTSTADTQVSNANT
                     SRSSTIQREISLSSMDIDALMQGNYDSILGTWQNSQGNSLVFNSKGLVADSQSLLGRG
                     KITDGIFETGYVDATIGDVTLLMIPKGTQANTTETDTTDSTRDRMIVISQETTADVVD
                     VYYRLTNSATSGIDPLKNTETGVQLDSGPKTIDYANSILGENNWRVIEGNYTRTESIP
                     YNILEGDDNARYTIYQNGVIINADYQIVYQP"
     gene            complement(2977..3735)
                     /locus_tag="AC1PIW_001747"
     CDS             complement(2977..3735)
                     /locus_tag="AC1PIW_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002934991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIP6606643.1"
                     /translation="MKRNVLITGASSGIGKAIAYVFAANGDNIIITGRRLDKLEEIKQ
                     DIEKQYHVQVSVHSFDVTNMEQTIVNCQKILEEVGQIHILVNNAGLALGLDKFQDYDL
                     TDMMTMIDTNIKGLLTVTRQLLPQMVENNEGHIINIGSTAGIYAYAGAAVYAATKSAV
                     KVLSDGIRIDTIDKNIKVTTLQPGIVETDFSQVRFHGDKERAATVYQGIDALQANDIA
                     SCTLFVANQPAHVQISDMTIMATKQATGFTIHRD"
BASE COUNT         1111 a          718 c          733 g         1227 t
ORIGIN      
        1 ttgatattta tatatgataa tatatgactg aaagcgtttt agaaaggtgt gatggctgtg
       61 ctgaaatact cgaaagtgct gttacttgtc ttgcttattg ctactggtct atcttgtatt
      121 ggaatctact ggctcggaaa agagcaaaat agactattaa atgagcagtg tcatgccttg
      181 aacatacgca ttattaatga tctgggaaca aaaattgatg ccattggcgg tccgcaaaat
      241 ccgcgtatca ttggattctt tcagcaagat gacactactg ctatcagcca gcggattggt
      301 acagcttctg aggaggaact gaaaatagct aaacctgaca atctattcca gaaagagtgg
      361 attgttctct atcctcaaac gcgcagcagt ccctttgaaa atgcttctgc ctatgcagta
      421 atgaaaacca gtatcaaggc tgactggctc catgtgacta cttcgtcaga gacggaatta
      481 gatattttct atgagaaggc agatgaaagc ttgttgacct tggaagactt ggtgcaagac
      541 aaggaaagtt ttcgtgctac actaaaaaca atcctagttt cagctaaaaa tgaggctgaa
      601 attcaagttc aaaaagatat tttagaaatg ttcgagagtg atgattggtc tgctattccc
      661 tttgcttaca ctgaaaaatc tctgatattg gagaaagctg tcatttcaat ttctgcattt
      721 gtagatagtc taaatcccta ctatttttca gaacaaaccc ttgcggattt gcgtttatca
      781 gaagaatcgc gccaggcttt agaagattcg gtagataaga caatcatcac ctatccttga
      841 ataaacggtg ggagtgggaa agtcgtcatg gatgtctttc ccagcttctt tatatctatg
      901 tttaacgaat taaacatttt tgtggatttg aaaaatattt tttgttatac ttgtgttatg
      961 aaaacatcaa cacttggaaa gttactagaa ttaccaaatt ttcaccactt ctacaccgtt
     1021 ccaattagag aaaatacgga gtcttctgga aattatataa ttgtttttga acaggacaag
     1081 caacctgtat ttgttgattt gaaaaatgag actggtcaca ctcgtgaggt acgctttgcc
     1141 agtcattttg acagcgccgg tcttctggta ggattaggag ccattcctat caattattgg
     1201 tctacctgcc agattcttgg tgtgaagaaa aataataatc gacgagggct tagtctatct
     1261 agtctgatgg atggttatag acgtctacct atttttaagg aaatggcaga gcgttataga
     1321 gcgcgtggac aatcaaccat ttatgacccc taccgttatt catatactat tgatgaaaag
     1381 aaggttatta aggataatca attaaccctt attccaaaga aagaatacga aaagataaaa
     1441 gatttccatc cttttttgca aacaaagcat ggaaatgttt atgcagttcc ttgtttctat
     1501 caagacagaa aacgaaatca gattactaaa atctatatta tgggctggta cttttatgag
     1561 aagcaattaa aaatatcttg gaaaccttct cattctcgta aaagccgctg gcgttcttct
     1621 cttgtttcag ttgataaatt atcggaccgc ttcttctttc aaggagaatt gtataaaaaa
     1681 ggaacctatc cagtgttcct ggaagatctc acaataaaaa ataaggccta ccaacatcgc
     1741 ctagataggg agtttgcagc ctttttaagc attattgaag aacaaaaaca gagagcacag
     1801 ctccgtgtta agtagaaaat gggtaggttg gtaaattacc gactatcatt tttacgatta
     1861 tcttttagtt ttacaacgac tcgaaactgg cgatcaggat gattttctgt attggacatc
     1921 tgaatacgtg cctctagaat atcgccgtgt ccaaaataga ttaagcgact gattagtgag
     1981 tttttatccg atgggatata tcctaacttt gtgccctgat aaaagattgc tacagcttct
     2041 gagtcatgtg ggtttgaagg ttctccgact agctgaacag gtgttcctgg cttgagttgg
     2101 tcgattacat ctagtccatc atagtaggca aatgctgcca aatgaaaatc ttgcacattg
     2161 cgtgttggtt caaatgatgt gaatgtcaca tctaacctcc tagagaatca ttatggttga
     2221 taaacaattt gataatctgc attgataata acaccgtttt ggtaaatagt gtaacgggca
     2281 ttatcatcgc cttccaaaat attataagga atagattcgg tgcgcgtgta gttaccttca
     2341 atgacgcgcc aattgttttc gcctaaaatt gagttggcat aatcaatcgt ttttggtcca
     2401 ctatccaact ggacgcctgt ttcagtattt tttaatgggt ctataccgct agtggctgaa
     2461 ttggtcaagc gataatagac atctaccacg tctgctgttg tttcttggct tattacaatc
     2521 attctatcgc gcgtagagtc agtagtgtct gtctcagttg tatttgcttg tgttcctttg
     2581 ggaatcatta atagtgttac atcgccaatc gttgcgtcta catagcctgt ttcaaaaatt
     2641 ccgtcagtta tttttccgcg gccaagtaag gactggctat ctgctactaa tcctttgcta
     2701 ttgaagacta gtgaatttcc ttggctattt tgccaggtgc ctagtatact atcgtaattg
     2761 ccttgcatta gagcatctat atccatagaa ctaagagaaa tctcacgttg gattgttgat
     2821 gacctgcttg tattggcatt agaaacctgg gtatcagcag ttgaggtact tgcggaactg
     2881 cccttctttt cagtttgaca ggcagaaagc gacatgatag tgagcaagcc aagagcgagt
     2941 gctaaatatt ttcttttcat tctagctatc tcccctctaa tctctatgaa tagtaaagcc
     3001 agttgcctgt ttggttgcca taatggtcat atcagaaatc tgcacatgtg caggttgatt
     3061 tgcaacaaat agagtacagc tggcaatatc atttgcttga agagcatcaa ttccttgata
     3121 gactgtcgct gctctctctt tatctccatg aaaacggact tggctaaaat cagtctcaac
     3181 gataccgggt tgtaacgtag ttaccttgat atttttatca atcgtgtcaa tacggatccc
     3241 gtctgaaagc actttgacag ctgatttggt tgcagcatag acagcagctc ctgcataggc
     3301 gtaaataccg gccgttgatc caatattgat aatatgaccc tcattatttt caaccatttg
     3361 tggcaataac tgacgtgtca cagttagtaa tcctttaata ttggtatcaa tcattgtcat
     3421 catgtcagtc aaatcatagt cttggaattt gtccaatccc aatgccaggc cagcattgtt
     3481 taccaaaata tgaatttgac caacttcttc taaaattttt tgacaattta caatcgtttg
     3541 ttccatattc gtaacatcaa atgaatgaac tgacacctga acatggtatt gcttttcaat
     3601 atcttgctta atttcttcta gtttgtccaa tcttcgtcct gtaatgatga tattatcacc
     3661 attagcagca aacacataag cgattgcttt gccaatacca ctacttgcac ctgtaatcaa
     3721 tacatttcgt ttcatcactc tgtctcctat tctctttatt atactacaat tttacctagt
     3781 ttgtaaaaa
//
LOCUS       DBNFCK010000136         3787 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.136, whole genome shotgun sequence.
ACCESSION   DBNFCK010000136 DBNFCK010000000
VERSION     DBNFCK010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.136"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            61..1341
                     /locus_tag="AC1PIW_001748"
     CDS             61..1341
                     /locus_tag="AC1PIW_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HIP6606644.1"
                     /translation="MTREFSFETLQLHAGQEVDSASKSRAVPIYQTTSYVFEDAQEAE
                     DLFALRKSGNIYTRITNPTVSTFENRVAALEGGIGALATSSGMAAITYTILALAHAGD
                     HIVAATTLYGGTFNLFKETLPRYGITTSFVDIDDFEAVEAAIKDNTKLVFIETLGNPV
                     INIPDLEKLAEIAHTYHIPLVSDNTFATPYLINVFSHGVDIAVHSATKFIGGHGTTIG
                     GVIVDSGKFDWAASGKFPQFVEEDPSYHNISYTRDIGVAAFIVAVRVQLLRDTGAALS
                     PFNAFLLLHGLETLSLRVERHVSNAEKIVDFLLNHPNVESVNYPSLPDSPYKALADKY
                     LPKGVGSIFSFQVKGGAEEARKVIDGLEIFSNLANVADAKSLVVHPATTTHAQLAPED
                     LLAAGVTPNQIRLSVGLENINDLIEDLQLALDKL"
     gene            complement(1422..2954)
                     /locus_tag="AC1PIW_001749"
     CDS             complement(1422..2954)
                     /locus_tag="AC1PIW_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIP6606645.1"
                     /translation="MLLTTHHLSLDHQKDLRNLVQDLNLIVNPGDKIAIIGEEGNGKS
                     SLLLTLMDSTLVADYLLQSGSIHRYFHSPVYIPQSLPLEIAELTLNDYFFADLDSDID
                     YSLLYLYADQLQFDSERFASQQLIESLSGGEKLKIQLIKSLATPSDIIFLDEPSNDLD
                     LDTLLWLENYTITSPKTILFVSHDEDFLSRTATKIIHLESVKKKTLAQTKVEELDYQD
                     YALRRQQAYQKQVQQARNDQKEFDKAMNKHLRQKSQVRHTLLNTHDATLGRLIAKKMK
                     NVLSREKRYERMKENLTQVPFHEDAISLFFSDISPLPARKQVLHLENFQLNVEERQLV
                     SNIHFNINGQEKIGIIGQNGIGKSSFLKLIYQKLRHRADINLGYMPQRYSEVLDESKT
                     PLDFLLENGNREQEQLILTHLASLQFTRQEVHHKICELSGGQKAKLLLLKMVLDQANF
                     LLLDEPSRNFSPTSQPYIRQLFADYPGGLVCVSHDRRFLKEVCQRIYRLTENGLEELG
                     QI"
     gene            3253..3744
                     /locus_tag="AC1PIW_001750"
     CDS             3253..3744
                     /locus_tag="AC1PIW_001750"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIP6606646.1"
                     /translation="MAITGIEQADILEIDKQVRLRRYDGQHDFSFEWHQDLELVWLID
                     GDQERYSLDLLNRMYDYLSKNGECYFIEIFEDNQFIPIGDVTLFADDFAIAIGDKRYW
                     KKGIGTKVLQRMIERAREVGLEEIPVEEIYDWNTGPRKLFEKCGFEAVEKTKKGWSYK
                     MIL"
BASE COUNT         1096 a          665 c          818 g         1208 t
ORIGIN      
        1 gtattagtca agatggtaaa aatgaggtag aatacagaaa aagaccaaag gagtaccttt
       61 atgactagag aattttcatt tgaaaccctt caactccatg ctggacaaga agttgattct
      121 gcttcaaaat cacgtgcagt acccatctat caaaccactt cctatgtttt tgaagatgct
      181 caagaagcag aagatttatt tgcattgcgt aaatcaggga atatctatac tcgtatcact
      241 aatccgacgg tctctacttt tgaaaatcgt gtggcagctt tagaaggtgg tattggtgcc
      301 ttggccactt cttcaggaat ggcagccatc acatacacta ttctagcatt agcgcatgct
      361 ggtgatcaca ttgtggcggc aacgacactc tatggtggga cttttaacct attcaaagaa
      421 acccttcctc gctatggtat tacaacaagc tttgtagata ttgatgattt tgaagcggta
      481 gaagctgcga ttaaagataa tacaaaactg gtatttattg aaaccttggg aaatcctgtt
      541 atcaatattc ctgatcttga aaaattggca gaaattgcac acacctacca catcccactt
      601 gtttcagata ataccttcgc aaccccatat ttgattaatg tattttctca tggtgttgat
      661 attgccgtcc attcagcaac caagtttatc ggtgggcatg gtacgaccat tggcggtgtg
      721 attgtcgata gcggtaagtt tgattgggcg gcatctggta agtttccgca atttgttgaa
      781 gaagatccaa gctatcataa cattagttat actcgtgata ttggtgtagc tgcatttatt
      841 gttgcagttc gtgtacaatt acttcgcgat acaggtgcag ccctttctcc attcaacgcc
      901 ttcttgcttt tgcatgggtt agaaaccttg tctctccgtg tagagcgtca tgtgtcaaat
      961 gctgaaaaga ttgttgattt cttgctcaat caccctaatg ttgagtcggt caattatcca
     1021 tcacttccag atagtcctta taaggctttg gcagataaat acctgcctaa aggtgttgga
     1081 tctattttct ctttccaagt aaaaggcgga gcagaagaag cccgaaaggt tattgatggc
     1141 ttggaaatct tttctaattt agcaaacgtt gccgatgcga aatcactagt agttcatcca
     1201 gctacaacca cgcatgctca gttagctccg gaagatttac ttgctgctgg ggtcacacca
     1261 aatcaaatcc gcctatctgt tggtcttgaa aatatcaatg atttgattga agatttgcaa
     1321 cttgctcttg ataaattata ggttgtgaat cgaatagctt ggcttcattc tttatgaata
     1381 aataataaaa ggaaatttgg cgtgtgtgcc attttccttt tttatatctg tcctagttct
     1441 tccagtccat tctcagttag tcgatagatt ctttgacaaa cttctttcaa aaagcgtctg
     1501 tcgtgtgata cgcataccaa accacctgga taatcggcaa atagttgacg aatatatggc
     1561 tgagaagtgg gagagaagtt tcgactgggt tcatctaaca agagaaaatt ggcttgatca
     1621 agaaccattt ttaacaagag taacttggct ttttgtcccc ccgaaagttc acagatcttg
     1681 tgatgaactt cttgtcgtgt aaactgcaga ctggctagat gtgttaagat aagttgctcc
     1741 tgctcacgat tgccattttc taataaaaaa tcaagtggag ttttagactc atcaaggact
     1801 tctgaatagc gctggggcat gtatcccaag tttatatctg ctcggtgcct gagtttctga
     1861 tagattagct ttagaaaact ggatttacca attccatttt gtccaatgat gccaattttc
     1921 tcttgtccat ttatgttgaa atggatattg ctaactaatt gtcgttcttc aacgttcaat
     1981 tggaagtttt ctaagtgaag cacttgtttt ctagctggta gtggtgaaat gtctgagaag
     2041 aagagggaaa tggcatcttc atgaaaagga acttgtgtaa gattttcctt cattctttca
     2101 tagcgttttt cacgtgagag aacatttttc attttcttag caatgagccg tcctagtgtg
     2161 gcatcatggg tgttgagcaa ggtgtgacga acctgtgatt tttgacgtag gtgcttattc
     2221 atggctttgt caaattcttt ttggtcgttc cgagcttgct ggacctgctt ctgataagct
     2281 tgctggcgtc tcaatgcgta atcctggtaa tctagttctt caaccttggt ctgtgcaagt
     2341 gttttctttt taaccgattc tagatgaatg atttttgtag ctgttctgct aagaaagtct
     2401 tcatcgtgag aaacaaatag gatggtttta ggactagtaa tggtatagtt ttcaagccaa
     2461 agtaaggtat ctaaatccaa gtcattggac ggctcatcca gaaaaataat atcagatgga
     2521 gttgctagag atttgattaa ctgaattttt agtttttcac cacctgacag gctttcaatc
     2581 aattgttgac tggcaaatcg ctcactgtca aactggagct ggtcagcata gaggtagagt
     2641 aggctgtaat caatgtcgct gtccaagtca gcgaagaagt agtcgttcag ggttagctct
     2701 gctatttcca gtggaagact ttggggaata tagactggtg agtgaaaata tcggtgaata
     2761 ctgcctgatt gaagcaggta gtcggcgaca agagtggaat ccatcaaggt gagtaataga
     2821 ctggattttc cgtttccttc ttcgccgatg atagctattt tatcgccagg gttaacaatg
     2881 aggtttaggt cctgaactag atttcgcaaa tctttttgat ggtcgagaga taggtgatga
     2941 gtggttagta gcataaggcc tcctttacaa gaaaaaacac actacctatt tagtagtgtg
     3001 tcgaaatctg ctcaaaaaga ccctattcat ggaacagaga aacagaaaaa tggacgcaag
     3061 aaaactacta aataagtata gaacttaaat tgagtttact tgttattgtc catttctcct
     3121 tacctcgcat gctttatttg ttttaatata gcatatcgat agaatcttgt caatcagcgt
     3181 gctataatat tcatagattt cgatagagat atatgttaaa atgaacttat tgtaaatgag
     3241 agaggatccg aaatggcaat cactggaatt gagcaagcag atattttaga aatagacaaa
     3301 caagtccgtt tacgtcggta tgatggtcag catgattttt cttttgaatg gcaccaagat
     3361 ctagaattgg tctggctgat tgatggtgat caagaacgtt atagtcttga cttgctcaat
     3421 cgtatgtatg attatttgtc taaaaatggc gaatgttact ttattgaaat ttttgaagac
     3481 aatcagttta tcccaattgg tgatgtgacg ctgtttgcag atgattttgc cattgccatt
     3541 ggagataagc ggtattggaa aaagggaatt ggaaccaagg tattacagcg aatgattgaa
     3601 cgtgcaagag aagtggggct tgaggaaata cctgtagaag aaatatatga ctggaataca
     3661 ggcccccgta aactgtttga gaaatgtggc tttgaagctg ttgaaaagac aaagaaaggt
     3721 tggtcctaca agatgatatt gtaaattaat tttgagatac caaaaaaaac ttgacgaaag
     3781 tcaaagc
//
LOCUS       DBNFCK010000137         3760 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.137, whole genome shotgun sequence.
ACCESSION   DBNFCK010000137 DBNFCK010000000
VERSION     DBNFCK010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.137"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(103..1476)
                     /gene="dnaA"
                     /locus_tag="AC1PIW_001751"
     CDS             complement(103..1476)
                     /gene="dnaA"
                     /locus_tag="AC1PIW_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HIP6606647.1"
                     /translation="MNQEQLFWQRFIELAKVNFKPSIYDFYVADAKLLGINQQVANIF
                     LNRPFKKDFWEKNFEELMIAASFESYGEPLTIQYQFTEDEQEIRNTTNTRSSIVHQVQ
                     TLEPATPQETFKPVHSDIKSQYTFANFVQGDNNHWAKAAALAVSDNLGELYNPLFIFG
                     GPGLGKTHILNAIGNKVLADNPQARIKYVSSETFINEFLEHLRLNDMESFKKTYRNLD
                     LLLIDDIQSLRNKATTQEEFFHTFNALHEKNKQIVLTSDRNPDHLDNLEERLVTRFKW
                     GLTSEITPPDFETRIAILRNKCENLPYNFTNETLSYLAGQFDSNVRDLEGALKDIHLI
                     ATMRQLSEISVEVAAEAIRSRKQTNPQNMVIPIEKIQTEVGNFYGVSLKELKGSKRVQ
                     HIVHARQVAMFLAREMTDNSLPKIGKEFGNRDHTTVMHAYNKIKTLLLDDENLEIEIT
                     SIKNKLR"
     gene            complement(1679..2443)
                     /locus_tag="AC1PIW_001752"
     CDS             complement(1679..2443)
                     /locus_tag="AC1PIW_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIP6606648.1"
                     /translation="MEELRTLNISEIHPNPYQPRIHFDEKELLELAQSIKENGLIQPI
                     IVRKSSIIGYELLAGERRLRASQLAGLTTIPAVVKELTDDDLLYQAIIENLQRSNLNP
                     IEEAASYQKLISRGLTHDEVAQIMGKSRPYISNLLRLLNLSSQTKQAVEEGKISQGHA
                     RQLVSFSEEKQAEWVQLILSKDLSVRTLEKLIAANKKKHTKLKQRDQFLKEQEDSLSK
                     TLGTATKIIKKKNGSGEIRISFNDLDEFERIINNFK"
     gene            complement(2504..3700)
                     /locus_tag="AC1PIW_001753"
     CDS             complement(2504..3700)
                     /locus_tag="AC1PIW_001753"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIP6606649.1"
                     /translation="MERIPYMKKYLKFAILFVIGFFGGLIGALSASFFQPQVQQANSA
                     ITSVSNVQYNNETSTTKAVEKVQNAVVSVINYQKSANNSLGVIFGNIESSDELAVAGE
                     GSGVIYKKYGQYAYIVTNTHVINNAEKIDILLASGEKISGELVGSDTYSDIAVIKISA
                     DKVTAVAEFADSDTIKVGETAIAIGSPLGSVYANTVTQGIISSLSRTVTSQSKDGQTI
                     STNAIQTDTAINPGNSGGPLINTQGQVIGITSSKITSSSANSSGVAVEGLGFAIPAND
                     AVAIINQLEKTGQVSRPALGVHMVNLTTLSTSQLEKAGLSNTELTSGVVIVSTQSGLP
                     ADGKLETFDVITEIDGEAIQNKSDLQSALYKHQIGDTITVTYYRNNQKQTVDIKLTHS
                     TEELSE"
BASE COUNT         1091 a          681 c          674 g         1314 t
ORIGIN      
        1 gtattctgtg gaaaagtgat gaaagaccaa taaaactgcg gaaattaact tgttcacaac
       61 ttgtggaaaa aaacagagtt ttttaaaaaa gttatacaca ggttaacgaa gtttattttt
      121 tatactggta atctctattt ctaaattctc atcatccaag aggagagttt ttattttatt
      181 gtatgcatgc ataacggttg tatggtctcg attaccaaat tctttcccaa tttttggaag
      241 ggaattgtct gtcatttcac gtgctaaaaa catagcaact tgtcgcgcgt gaacgatatg
      301 ttgaacacgc ttagaacctt ttaattcttt caagctgaca ccgtagaaat ttcccacttc
      361 ggtttggatt ttctcaatag gaataaccat gttttgtgga tttgtttgtt ttcttgatcg
      421 aatagcctca gcagcaacct cgacacttat ctcagacagt tgacgcatag tggctatcaa
      481 atggatatct tttaaggcac cttcaaggtc acgtacgttc gaatcaaatt gcccagctag
      541 ataggatagc gtctcatttg taaagttgta aggcaggttc tcgcacttgt tacgtaaaat
      601 tgcgatacgt gtttcaaaat caggtggagt gatttcactg gttaaccccc atttgaaacg
      661 tgttactagt ctttcttcca aattgtctaa gtgatcggga ttacggtcgc ttgtgagtac
      721 aatctgctta tttttttcat gaagcgcatt aaaagtatgg aaaaattctt cctgtgttgt
      781 tgctttatta cggagagact gaatgtcatc aattagaagt aagtccagat tgcgataggt
      841 ttttttgaaa ctttccatat cattgagacg gaggtgttct aaaaattcat tgatgaatgt
      901 ttccgatgag acatatttta tccttgcctg gggattatcg gctagaacct tatttccaat
      961 cgcatttaaa atatgagttt ttccaagacc aggaccacca aaaatgaata atggattgta
     1021 gagctcacct aggttatcag atacagctaa agctgcagcc tttgcccagt gattattgtc
     1081 tccttgtaca aaattagcaa aggtgtactg ggattttata tcagaatgaa ccggtttaaa
     1141 agtttcttga ggagtagccg gctcaagtgt ctgtacctgg tgaactattg aacttcttgt
     1201 gtttgtagta ttcctaatct cctgttcatc ctctgtaaat tgatattgga tggtaagagg
     1261 ctctccgtag ctttcaaaac tagcggcaat cattaactct tcgaagtttt tttcccagaa
     1321 atctttttta aatggacgat ttaagaaaat attggcaact tgctggttga ttccgagtaa
     1381 ttttgcatca gcgacataaa aatcataaat agatggctta aaatttacct ttgccaattc
     1441 aataaatcgt tgccaaaaaa gttgttcttg gttcatattt tccccctttc acattatatt
     1501 cgttagaata gtgtaccata aaaaacaaaa gttttccaca gataattttt aaaatccaca
     1561 ctgttaagaa aagttttcca caagatgtga ataaaagtca gaacccttgc tggtcctgac
     1621 tttttgtaat gattttagtg tggaaaagag tgtttataaa aataaattgt aaacaagtct
     1681 atttaaaatt gttgataatt ctttcgaatt catcgaggtc attaaagcta atccgaattt
     1741 ctccgctccc gtttttcttc ttgataattt ttgtagctgt tccaagagtt ttactgagtg
     1801 aatcttcctg ttcttttaaa aattggtcgc gttgtttaag cttagtgtgt tttttcttat
     1861 ttgcagctat taatttttca agcgtacgca cacttaaatc ctttgataaa atgagttgaa
     1921 cccattcggc ttgcttttct tctgaaaatg acaccaattg tcgcgcgtgc ccttgtgaaa
     1981 tttttccttc ttctacagct tgtttagtct gagatgatag atttagtagg cgcaataaat
     2041 tactgatata tggtcttgat tttcccatga tttgagcaac ttcatcatgt gttaaccctc
     2101 tactaatcaa tttttgataa gaggctgctt cttctatcgg atttaagtta gaacgctgca
     2161 gattctctat gatagcctga tagagtaaat catcatcagt cagttctttt accactgctg
     2221 gtattgtagt cagtccagct aattgactgg ctcgcaacct tctttctcca gctaataatt
     2281 cgtatccgat aatagaagat tttcttacaa taatcggttg aattaagcca ttttccttaa
     2341 tagattgagc gagctcaagt agctcctttt catcaaaatg aattcttggc tgatagggat
     2401 tgggatggat ttctgaaata tttagtgtac gtaattcttc catatatcct tattctagca
     2461 aaaaaaatga gactttacaa ttgtgtaaag tctcatttgt caattattcg ctaagttctt
     2521 ctgtagaatg tgtcaactta atgtcaacag tttgtttctg attattgcgg taataagtta
     2581 cagtgattgt atctccaatt tgatgtttgt agagagcgct ctggaggtca ctcttatttt
     2641 gaatagcttc tccgtcaatc tcagtaataa catcaaaagt ttctaatttt ccatctgcag
     2701 gtagcccact ttgtgtagag acaattacta caccggatgt taattccgta tttgataatc
     2761 cagctttttc taattgacta gttgacaagg tcgtcaagtt aaccatatga actccaagag
     2821 caggtcggct aacttgtcca gttttttcaa gctgattgat aatagctacg gcatcatttg
     2881 caggaatagc aaatcccaac ccttctacag ccacgcctga gctatttgca gaacttgagg
     2941 taattttgct agaggtaatg cctatcactt gtccttgggt attgattaac ggtccgccag
     3001 agtttccagg gttgatagct gtatcagttt gaatagcgtt agttgagatt gtttgtccat
     3061 cttttgattg tgaagtaact gtccgactta agctagaaat aataccctgg gtaactgtat
     3121 tggcgtagac gctacctaga ggactaccaa ttgcgatagc agtttctcca actttaattg
     3181 tatcggaatc agcaaattca gcaacagcag tgactttatc tgctgatatt tttataacag
     3241 ctatatcaga atatgtatcg gaaccaacaa gttcaccgct aattttttct ccagatgcta
     3301 aaaggatatc aatcttttct gcgttattaa taacatgcgt atttgtcaca atataggcat
     3361 attgaccata ttttttatag ataaccccag acccctctcc agcaacagct agttcgtcag
     3421 atgattcaat atttccaaag ataacaccaa gactattgtt ggctgatttt tggtaattaa
     3481 taacagacac aacagcattt tgtactttct ctacagcttt tgtggtggaa gtttcattat
     3541 tatattgaac attgctgaca ctagtgatag cagaatttgc ttgttgcacc tgtggctgga
     3601 agaaagaggc tgacaaggcc ccgataagac ccccaaaaaa tccaattaca aataaaatcg
     3661 caaatttcaa atattttttc atataaggaa tcctttccat ttctttaatt ttccttaaga
     3721 gtactatttc tttcttaatt ataacttaaa gttttcaaaa
//
LOCUS       DBNFCK010000138         3714 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.138, whole genome shotgun sequence.
ACCESSION   DBNFCK010000138 DBNFCK010000000
VERSION     DBNFCK010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.138"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..177)
                     /locus_tag="AC1PIW_001754"
     CDS             complement(<1..177)
                     /locus_tag="AC1PIW_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HIP6606650.1"
                     /translation="MEINTHNAEILLSAVSKAQYPGYDIPEIALAGRSNVGKSSFINT
                     LLGRKNLARTSGKPG"
     gene            complement(233..1459)
                     /gene="clpX"
                     /locus_tag="AC1PIW_001755"
     CDS             complement(233..1459)
                     /gene="clpX"
                     /locus_tag="AC1PIW_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIP6606651.1"
                     /translation="MAVKHTHELIYCSFCGKNQEEVKKIIAGNNVFICNECVELAQEI
                     IREELAEEVLTDLADTPKPQELLNILNNYVIGQDRAKRALAVAVYNHYKRINFQDSRD
                     ENDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVENIL
                     LKLLQAADFNIDRAERGIIYVDEIDKIAKKGENVSITRDVSGEGVQQALLKIIEGTVA
                     SVPPQGGRKHPNQEMIHVDTKNILFIVGGAFDGIEEIVKQRLGEKIIGFGHNNRAIDE
                     KESYMQHIIADDIQKFGIIPELIGRLPVFAALDQLTTEDLVRILTEPKNALVKQYQTL
                     LSYDDVELDFDEDALLAIAEKAIERKTGARGLRSIIEETMLDVMFEVPGQDNVKRVRV
                     TKAAVDGKEKPLLETA"
     gene            complement(1481..1654)
                     /locus_tag="AC1PIW_001756"
     CDS             complement(1481..1654)
                     /locus_tag="AC1PIW_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606652.1"
                     /translation="MERSLFGLFTAFLCGICVLCAIQAFRKKRFGLAILFLLNAFTNL
                     VNSIHAIYGFLFR"
     gene            complement(1657..2169)
                     /locus_tag="AC1PIW_001757"
     CDS             complement(1657..2169)
                     /locus_tag="AC1PIW_001757"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HIP6606653.1"
                     /translation="MTKKIVAIWAQDENGLIGKGDRLPWSLPADLAHFKKTTTGHTMV
                     MGRITFDGMGKRALPNRHTIVLTTDKTYQLESERITILHSVEDVLDWYNKQEQTLFVI
                     GGGQIFTAFAPYIETLIVTDIHGQFNGDVYFPKAFPMEKFQLQSANLRPKDEKNPYDF
                     TIKTYERRDG"
     gene            complement(2455..3294)
                     /locus_tag="AC1PIW_001758"
     CDS             complement(2455..3294)
                     /locus_tag="AC1PIW_001758"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HIP6606654.1"
                     /translation="MTKADIIFKENIRKIMEEGVFSENARPRYKDGNVANSKYVTGAF
                     AEYDLSKGEFPITTLRPIPIKSAIKEILWIYQDQTNDLSVLQEKYGVQYWNDWEVEGT
                     GTIGERYGAIVKKHDIISKILKQLEENPWNRRNVISLWDYEAFDQSAGLLPCAFQTLF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAEELLRRTPSEVEPRLVLNVPDGTNFFDIRAEDFELVDYNPVKPQLKFD
                     LAI"
     gene            complement(3347..>3714)
                     /locus_tag="AC1PIW_001759"
     CDS             complement(3347..>3714)
                     /locus_tag="AC1PIW_001759"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606655.1"
                     /translation="NLISSDIRYWLLYIWPLEGVVMNLTLLKRLNLVLYGIAIFLFVM
                     LFLPIGQWFDIVNVNFKLTFFIIPFFGLASLPTAIYTKNVRQILLSVLLVALYFILFS
                     LITALSGLFHLNFYSFFFK"
BASE COUNT         1105 a          783 c          689 g         1137 t
ORIGIN      
        1 tcctggtttt cctgacgtac gtgcaagatt ttttcttcct agaagagtat tgataaatga
       61 ggactttcca acgttagatc gcccggcaag ggcaatttct ggaatgtcat agcccggata
      121 ctgggctttt gaaacagcac tgagcaaaat ctcagcatta tgtgtgttga tttccatttt
      181 atacctttca taaataaggg tagaaaacaa tcagtttcca ccctttattt ttttatgcag
      241 tttctagtaa aggcttttct ttaccgtcta ctgctgcttt tgttacacga acgcgtttaa
      301 cgttatcttg acctggcacc tcaaacatga catccaacat cgtttcttca atgatagagc
      361 gaagtccacg agcaccagtt ttacgttcaa ttgctttttc tgcaattgct aacaaggcgt
      421 cctcatcaaa gtcaagctcc acgtcatcat aagacaagag tgtctggtat tgttttacaa
      481 gagcattctt cggttccgtc aaaatgcgta ccaaatcctc tgttgtcaat tggtccaaag
      541 ctgcgaatac aggcaagcga ccaatcaact ctggaatgat accgaatttt tgaatatcgt
      601 ctgcaatgat atgttgcatg tatgattctt tttcatcaat agcacgatta ttatgaccaa
      661 aaccgatgat tttttcaccg agacgctgtt tgacgatttc ttcgatacca tcaaatgcac
      721 cgccaacgat aaacaggata tttttggtgt ccacgtgaat catttcttgg tttggatgtt
      781 tacgtccgcc ttgtggtggt acgctggcca cagttccctc gataattttt aggagagctt
      841 gctggacacc ttcaccagat acatcgcggg taatggacac attttcacct tttttggcaa
      901 tcttatcaat ttcatctaca tagataattc ctctttctgc acggtcgatg ttaaagtctg
      961 cagcctgcaa aagtttgagg aggatatttt ctacgtcttc accaacataa cctgcctcag
     1021 taagagcggt cgcatcagca atagcaaacg ggacattcaa acttttagcc aaggtctgag
     1081 ctaggaaggt ttttcctgaa ccagtcgggc caatcatgag gatatttgat ttttgcaaat
     1141 caacatcgtt ctcatcacgg ctatcttgga aattgatacg tttgtagtgg ttgtaaacgg
     1201 ctactgctaa agcacgtttt gcacgatcct gtccgatgac gtagttgttc aggatattga
     1261 gtaattcttg tggctttggc gtatcggcca agtctgtcaa aacttcttct gccagttcct
     1321 cacgaatgat ttcctgtgct agttccacgc actcattaca aatgaagaca ttgttccctg
     1381 cgataatttt tttaacttct tcttgatttt ttccgcaaaa tgagcaatag attagctcat
     1441 gtgtatgctt aacagccatg tgtttcctct ttcatatctc ttatctaaat aaaaatccat
     1501 aaatggcgtg aatactattg accagattgg taaaagcatt cagtaaaaat aaaattgcta
     1561 aaccaaaacg tttcttccga aaggcttgaa tcgcacaaag aacacaaatc ccacagagaa
     1621 atgctgtaaa taaaccgaat aaacttctct ccatgcctaa ccatctcttc tttcataggt
     1681 ttttattgta aaatcatacg ggtttttctc gtcttttgga cgtaaattgg cgctttgtag
     1741 ctgaaatttc tccataggaa atgctttcgg aaaatacaca tcgccattaa attgaccgtg
     1801 aatatctgtc actattaatg tttcaatgta aggtgcaaaa gcagtaaaaa tttgtccccc
     1861 accaatcaca aatagtgtct gctcctgttt gttataccaa tctagaacat cttccacact
     1921 gtgtaaaatg gtaatacgtt cactctctag ttgataggtt ttatccgttg tcaaaacgat
     1981 ggtgtgacga ttgggaagag cacgtttacc catgccgtcg aaagttatac gtcccatcac
     2041 catcgtgtga cctgttgtcg tttttttgaa atgagctaag tcagctggca atgaccaagg
     2101 aagtctatca ccttttccaa tcaatccatt ttcatcttga gcccaaatgg caacaatctt
     2161 tttagtcatt ttctctcact ttccgtccta tcattctatc aaaaaaacat gaaaaatgca
     2221 cttaccaagt aggccaagcg caaattttta tcatgttgaa actatcaccg actcctcaga
     2281 gatagggaag attccgagat tcgtagaatg tgaatctcgc tggtgctgga aagcccccct
     2341 catcttaaat cgctaagtca aaattaagtt gcggtttaac tggcttcgaa agtctgagta
     2401 tagactgttg aaggttggaa atagcaagaa cgaagttctt cactgctaat ccagttaaat
     2461 tgctaaatcg aattttaact gcggtttaac tggattgtag tccaccaatt caaaatcttc
     2521 tgcccgaata tcaaagaaat tggtaccgtc aggaacattt aagaccaaac gtggctcaac
     2581 ttcagatggt gtgcgacgga gtaattcttc agcctgttca aactgattgt catagatgtg
     2641 aagattgttg atgaaataga aaaacttccc aaccttccag ccaaaatgtt tagcgatcat
     2701 catctgaagg gctacatact gcatagcgtt gatatgatgg gctaccaaca tatcatttga
     2761 ccgttgagtt agagttgcat ccagataaat ctccccatcc actcgccgaa cgtcaaacag
     2821 ggtctgaaag gcacatggca aaagacccgc ggattggtca aatgcttcat aatcccacaa
     2881 agaaatcaca ttacggcggt tccatggatt ttcctctaat tgcttgagga tttttgaaat
     2941 gatgtcatgc tttttcacga tagccccata gcgttcaccg attgtgcctg ttccttcaac
     3001 ttcccagtca ttccaatact ggacaccgta tttttcctgt aagacagaca ggtcattagt
     3061 ctggtcttgg taaatccaga gaatttcctt gatggccgac ttaattggaa taggtctaag
     3121 tgttgtaata ggaaactcgc ccttagacaa gtcatactca gcaaaggcgc ctgtaacgta
     3181 cttggaatta gctacatttc catccttgta acgtgggcga gcattttctg aaaaaacgcc
     3241 ctcttccata atcttacgaa tgttttcttt aaaaataata tctgctttag tcatggtctt
     3301 agtatagcaa attctagcaa aaaactccag ttttgcaccg gagttctcat ttgaaaaaga
     3361 atgaataaaa attaaggtgg aacaaaccag atagggcagt tattaggctg aacaatataa
     3421 aatacaaggc aaccaagaga acactcaaca atatctgacg aacattttta gtgtaaatag
     3481 ccgttggtaa gctggctagt ccgaaaaatg gaatgataaa aaatgttagt ttaaaattca
     3541 catttacaat atcaaaccat tgtcctattg gcaaaaatag catgacgaag agaaaaattg
     3601 cgataccata caaaactaga tttaatcttt ttagtaaagt caggttcata accactcctt
     3661 ctaggggcca aatataaagt agccaatacc gaatatcaga ggaaatgaga ttat
//
LOCUS       DBNFCK010000139         3597 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.139, whole genome shotgun sequence.
ACCESSION   DBNFCK010000139 DBNFCK010000000
VERSION     DBNFCK010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.139"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..476
                     /locus_tag="AC1PIW_001760"
     CDS             <1..476
                     /locus_tag="AC1PIW_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001213956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIP6606656.1"
                     /translation="VANERLFLEFNINSKETSQHLDILYKEVSVLSLGQKVVAMLDFL
                     LAYSGYSKDFRPLIIDQPEDNLDNRYIYRHLVQQFRDVKAQRQIILATHNATIVTNSM
                     TDQVVIMESDGAHAWIESQGYVSEKFIKNHIINQLEGGRDSFKHKMSIYETALSE"
     gene            814..1176
                     /locus_tag="AC1PIW_001761"
     CDS             814..1176
                     /locus_tag="AC1PIW_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013851909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1252 family conjugative relaxosome accessory
                     protein"
                     /protein_id="HIP6606657.1"
                     /translation="MTESYRTIRKEINLTLEEFEQIQLLMRKENAEQFSPFVRQKLLD
                     IVNNKDDIRDWFILWQSQKIEQISRDILQVATLAERGHQVTSEHLRIILTCVQELMTE
                     VEKAVPLSSEFRDKYMGG"
     gene            1179..1544
                     /locus_tag="AC1PIW_001762"
     CDS             1179..1544
                     /locus_tag="AC1PIW_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HIP6606658.1"
                     /translation="MENRYRTNLKKVFLTDQELANLNNHISQSSCQNFSAYARKVLLN
                     PNMTFLTIDTSNYQDLVFELRRIGNNINQIAKTVNQTKLLTVEQRDNLKTGIGQLIAE
                     VDKDFYVKCQKLREFYGSH"
     gene            1531..3414
                     /locus_tag="AC1PIW_001763"
     CDS             1531..3414
                     /locus_tag="AC1PIW_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1250 family conjugative relaxase"
                     /protein_id="HIP6606659.1"
                     /translation="MVVTKHFAVHGNKYRKSLIKYILNPAKTDQLKLVSDFGMSNYLD
                     FPNYEEIVEMYQANFVNNDRLYDSRNDRQQIKQNKIHAHHLIQSFSPEDNLTPEEINR
                     IGYETIKELTGGNFRFIVTTHTDRLHVHNHILINSVDLNSHKKLKWDYAQERNLRMIS
                     DRLSKEAGAKIITPNRYSHEKFVTYRKSNHKFELKQRLYFLMENSKDFDDFLSKAEAL
                     NVQIDFSRKYARFLMTDMPMKRVIRGKQLDKRQPYTEEYFREHFAKRAIEQCLDFLLP
                     RVRDLSQLLEFAREPNLAMSLKQKNVAFTLTKNSQSITVNNQKVSSKNLYDVQFFETY
                     FEKRGEVPDIDQSQLISDFEGYCEEQDKEKLAYEDLQEAYQAFKEKRDQVQEFDVVLA
                     DHQIDKLVKDGLFIRMNYGVKKDGLVFIPNRHLDIKETESGKHYHAFIRETAQFFIYN
                     KEASELNRYMRGRELIRQLTNDSQTISKRKRPTIDTLKKKIEEINLLIELGTENKSYQ
                     DIKDEIIKDIAQLDLTITEIQEHIDHLNKVAEVLLNLDNNDIENRRLAKYDYAKMNLT
                     AAIKLEQVEKEIEIYQIKLDKSIDDYEYLIRRLEKFVKILNEIDSSKDELKFEHSEKN
                     VKE"
BASE COUNT         1258 a          589 c          715 g         1035 t
ORIGIN      
        1 acgtagccaa tgaaagactt tttttggagt ttaacattaa ctcaaaagaa acatcacaac
       61 atcttgacat tctttataaa gaagttagtg tattaagttt gggtcaaaaa gttgtagcga
      121 tgttagattt tttactagca tatagtggtt attcaaagga tttcagacca ttaattattg
      181 atcaacctga agataatttg gataaccgct atatatacag acatttagtt caacagttta
      241 gagacgtgaa agctcaacga caaattattt tagcaaccca taatgcgaca attgtaacaa
      301 attccatgac cgatcaagtg gtaattatgg aatcagatgg ggctcatgct tggattgaat
      361 cacagggata tgttagtgaa aaatttataa aaaaccatat tatcaatcaa ttggaaggtg
      421 gaagagactc atttaaacat aagatgtcca tatatgagac agctctttct gaataatata
      481 aggaaatgaa aagtaggtta gaaatatcct actttttgtt ttgtgatacc ttttccaaat
      541 cacctctatt atcctaatat cattcctaaa aaaatcaatc catgattttt cactgggggt
      601 ttgggggcga agccaccaag ttatcttatc gtcggctatc aaaactcaaa ggttttggtc
      661 ggctggcgat aagatttttg ggatattgtg gacacaatat ctgagctcgc aaaagccgaa
      721 aagggaagat ttgaggcttt tagggagccc ccgtatcaga tatggtatcg gtgctaaccc
      781 gctagaggga aatgtgagaa ggagtgtatc atcatgacag aaagttatcg gacaattcgt
      841 aaggaaatca acttgacgct tgaggaattt gagcagattc aactactgat gagaaaggag
      901 aatgcggagc agttttctcc ctttgttaga caaaaactat tggacattgt caataacaag
      961 gatgacatca gagattggtt tattctgtgg caatctcaaa aaatagaaca aatcagtaga
     1021 gatattttac aggtagcaac tctagcggaa cgaggccatc aggtcacgtc agaacactta
     1081 agaattatat tgacttgtgt tcaagaactg atgacagaag tagagaaggc agtaccactc
     1141 agttcagaat ttcgagataa gtatatggga ggttagatat ggaaaatcgt tatcgaacca
     1201 atctcaaaaa agtcttttta acagaccagg aacttgcgaa cctgaacaat catattagcc
     1261 aaagttcttg tcaaaatttt tctgcttatg ccagaaaggt tttgcttaat ccaaatatga
     1321 cctttttaac aattgatacc agtaactacc aagacttggt ttttgaatta cgtcgaattg
     1381 gcaataacat taaccagata gctaaaacag tcaatcagac aaaacttctg acagtagagc
     1441 agcgagataa tttgaaaacg ggcataggtc agctgattgc ggaagttgat aaggacttct
     1501 atgtcaagtg tcaaaagttg agggaatttt atggtagtca ctaaacattt tgcggttcat
     1561 ggcaacaaat accgtaaaag tcttatcaag tatattctaa atccagctaa gactgaccaa
     1621 ctcaaattgg tttcagattt tggaatgagc aattacctcg acttccctaa ttatgaagaa
     1681 atagttgaga tgtatcaggc aaactttgtc aataatgata ggctttatga ttcgagaaat
     1741 gaccggcaac aaatcaagca aaataaaatt catgctcatc atcttatcca gtcattctca
     1801 cctgaggata atcttacacc tgaagaaatc aatcgtattg gttatgagac gataaaggag
     1861 ttgactggcg gaaactttcg ctttattgtg acaacccata cggataggtt gcatgttcat
     1921 aaccatatcc tcatcaattc tgttgacctc aattctcata agaaactcaa atgggattat
     1981 gctcaggaga gaaatttacg gatgatttct gaccgtcttt ccaaagaagc aggtgccaaa
     2041 attatcacac cgaaccgtta ttctcatgag aagtttgtga cctatcgcaa aagtaatcac
     2101 aaatttgaat tgaagcaacg tctttatttt ctgatggaga atagtaagga ttttgacgac
     2161 tttttatcca aggcagaagc attgaatgtt cagattgatt tttcaagaaa atacgctcgt
     2221 tttttgatga cggatatgcc catgaagcga gttattcgag gcaagcaact agataaacgt
     2281 caaccctata ccgaggaata ctttcgagag cactttgcca agcgagcgat tgagcaatgt
     2341 ttagattttc tcttacccag agttcgtgac ctttcacaac ttttggagtt tgcaagagaa
     2401 cccaaccttg ctatgtctct aaaacaaaag aatgtggcgt ttactctaac aaaaaatagt
     2461 cagagcatta cagtcaataa tcaaaaagtc agttccaaaa atctctacga tgttcaattt
     2521 ttcgaaactt actttgagaa acgtggagaa gttcctgata ttgaccagtc gcaacttatt
     2581 tccgactttg agggatattg tgaggaacaa gataaggaaa agctagctta tgaagacttg
     2641 caagaggctt atcaggcatt taaggagaaa agagaccagg ttcaagagtt tgatgtcgtc
     2701 ttagctgacc atcagattga taagttagtc aaagatggtc tctttatcag aatgaactac
     2761 ggcgttaaaa aagatggctt ggtctttatc ccaaaccgtc atttagatat taaagagaca
     2821 gagagtggta aacactacca tgcctttatc cgtgagaccg cacaattttt catttacaat
     2881 aaggaggcgt ccgaactcaa tcgctatatg cgagggcgtg aactcattcg tcagttaacc
     2941 aatgatagcc agactatctc taaaagaaaa agaccgacca ttgacacact taagaaaaag
     3001 attgaagaga ttaatcttct tattgagttg ggtacggaga ataagtctta tcaagatatt
     3061 aaagacgaga ttattaagga tattgctcaa ttagatttga ccattacaga gattcaggaa
     3121 cacatagatc atctcaacaa ggttgcggaa gtcttgctta acctggacaa taacgatata
     3181 gagaaccgcc gcttggccaa atatgactat gccaagatga atctaactgc ggctataaaa
     3241 ctggaacaag ttgagaaaga aatcgaaatc tatcaaatta agttagataa gtcaatagat
     3301 gattatgagt atttaataag gaggttggaa aagtttgtca aaattttaaa tgaaatagac
     3361 tcaagcaagg atgaactaaa atttgaacac agtgaaaaaa atgtgaaaga atagcatttt
     3421 tcggatttat tgataccaaa tattgagtac tctgaagaaa ttctgaaata aaattgataa
     3481 aaaggagtaa aaaactaagg agatgatatt atgaaaaaga taggcttatt gtcgaatgtt
     3541 tttatatcat tgtttattgt tatattagct agcggttctg tgtcatcagg agtaagt
//
LOCUS       DBNFCK010000140         3536 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.140, whole genome shotgun sequence.
ACCESSION   DBNFCK010000140 DBNFCK010000000
VERSION     DBNFCK010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.140"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(73..915)
                     /locus_tag="AC1PIW_001764"
     CDS             complement(73..915)
                     /locus_tag="AC1PIW_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HIP6606660.1"
                     /translation="MFTISNIRERARQTLNETPGIYQVAVIPVTISVIVQLISFSRNS
                     LAGVSAEELASPSYFISTSLFPVFYGLLLGMLSLSVIWTIFNIIKGGKTTTSFKDSLS
                     IFNHKDFWKIFRTYILKSFLLFLWGLLFYLGIGLLIGAGTMITIIAASSQSLDPDALP
                     MEILAILGLMAMGGILLMVLGIALYIPQIYAYSQVEYILFEQLEREEYTGAFSIIKSS
                     RKLMKGYKFKRFTLDLSFIGWFLLVIITFGLAGLYVWPYHYAAQMHFHEEILDDQAKK
                     MSYV"
     gene            1235..2377
                     /gene="tgt"
                     /locus_tag="AC1PIW_001765"
     CDS             1235..2377
                     /gene="tgt"
                     /locus_tag="AC1PIW_001765"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIP6606661.1"
                     /translation="MTDVPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTMSPEELKAMGSGIILSNTYHLWLRPGDELVARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNISEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFPGYSI
                     GGLAVGETHDEMNAVLDFTTPLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTNRGRLVVKNAQFAEDFTPLDPECDCYTCKNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLINLMKNVRQAIMDDNLLEFRQDFMEKYGYGKNGRNF"
     gene            complement(2416..3501)
                     /locus_tag="AC1PIW_001766"
     CDS             complement(2416..3501)
                     /locus_tag="AC1PIW_001766"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIP6606662.1"
                     /translation="MSKLHHVKSYLEANKMDLAIFSDPVSIYYLTGYHSDPHERHMML
                     FVMPDHDSLLFLPALDVERAVATVDFPVAGYMDSENPWQIIKSKLPQKSFSAICAEFD
                     NLNLTRYHGLQSIFSQPFSDITPLINTMKLIKSRDEIEKMLVAGEFADKAMQVGFNNI
                     SLDVTETDIIAQIEFEMKKQGISKMSFETMVLTGDNAANPHGIPSTNKIENNALLLFD
                     LGVEALGYTSDMTRTVAVGKPDQFKKDIYNLTLEAHMAAVNMIKPGVTAGEIDYAARS
                     VIEKAGYGEYFNHRLGHGLGMSVHEFPSIMEGNDLVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGYVTKNGFEVFTKTPKELLYFEG"
BASE COUNT         1096 a          682 c          742 g         1016 t
ORIGIN      
        1 attactatat tttttttctt aaatatagac ataaaagcgt atataaaaat aagagcaaat
       61 atcgctctta ttttatacgt aactcatttt cttagcctga tcatctaaaa tttcttcatg
      121 gaagtgcatt tgtgcagcat agtgatacgg ccatacatat aaaccagcca ggccaaatgt
      181 aataatgaca agaaggaacc agccgatgaa ggataaatca agtgtaaagc gtttaaactt
      241 gtaacctttc atcaactttc gactagattt aatgattgaa aaggcacctg tatattcttc
      301 gcgttccaat tgttcaaaca agatatattc tacctgtgag taagcataga tttgtggaat
      361 ataaagagca attcctaaaa ccataagtag gatacctccc atggccatta gccctagtat
      421 ggcaaggatt tccataggaa gtgcatcagg gtctaaggat tgactggatg cagctataat
      481 cgtaatcatc gttccagcac cgataagtag accgatacca aggtaaaaca gtagtcccca
      541 aaggaagagc aagaaacttt ttaaaatgta agttcgaaag attttccaga aatctttatg
      601 attgaaaatg ctcaaggaat ctttgaatga tgtggtagtt ttgccccctt tgatgatatt
      661 gaagatagtc catatgactg ataatgacaa cattcctagt agcaatccgt aaaatactgg
      721 aaacaaagag gtagaaataa agtagctcgg actagcaagt tcctcagccg acactccagc
      781 aaggctattg cgagagaaag aaattaactg aacgataaca gaaatagtga cggggatgac
      841 ggccacttga tagataccag gtgtctcgtt tagcgtttgg cgagcccgct cacgtatatt
      901 agaaattgta aacatctaca taacctcctt acccaacatt gtaacataat tctagatgac
      961 aagctaggct ttttttgata gactagaagg tgctgtggga atttgtcagt ttgttgtgat
     1021 gcgcctatct atttactatt aaataagtag aagaaaaggc aaatatcact gaaataagcg
     1081 agaacattaa agacattctg atagattaga ccatgcaaga ttgagaaact gtatttccag
     1141 gtaaagtgct tttatggatg gtatgaaaga gctgaactgt gaatccagaa ctaatatcct
     1201 aggactgttt ttcctagcgg gaggtaaata aaacatgaca gatgttccaa tcaagtatcg
     1261 cttgattaag aaagaaaagc acacaggtgc acgtttgggc gaaatcatta ccccacacgg
     1321 gacctttcca acccccatgt ttatgccagt tggaactcag gcaacggtaa aaaccatgtc
     1381 gcctgaagaa ttgaaagcga tgggttcagg aattatccta tctaatacct atcatttgtg
     1441 gcttcgtcca ggtgacgaat tggtggcgcg tgcaggtggt ttgcataaat ttatgaattg
     1501 ggatcaaccg attttgactg actcaggtgg tttccaggtt tattctttgg cggatagtcg
     1561 caatatctca gaagaaggtg tgaccttcaa aaaccatctc aatggtagta aaatgttctt
     1621 gtcacctgaa aaagctatct caattcagaa taatcttggt tcggatatta tgatgagctt
     1681 tgatgagtgt ccgcagttct accagcctta tgactatgta aaaaaatcca tcgaaagaac
     1741 aagtcgttgg gcagaacgtg gtctcaaggc tcaccgccgt ccgcatgacc aaggtttatt
     1801 tggtattgtc caaggtgctg gttttgaaga tcttcgtcgc caatcagccc atgatttggt
     1861 tagcatggat tttccaggat actctattgg aggattggct gttggtgaaa cacatgatga
     1921 aatgaatgct gtactggact tcacgacccc tcttttaccg gagaataaac cacgctatct
     1981 catgggtgtt ggagcaccag atagcttgat tgatggcgta attcgtgggg ttgatatgtt
     2041 tgactgtgta ttaccaacac gtattgcgcg taatggaacc tgtatgacca atcgtggacg
     2101 tcttgtagtg aaaaatgccc agttcgctga ggactttaca ccgttagatc cagagtgcga
     2161 ttgctacaca tgtaaaaact atacacgtgc gtatctccgc catttactta aggccgacga
     2221 gacgtttggt attcgtttga caagttatca caacctctac ttcttgatta atttgatgaa
     2281 aaatgtccgt caagcaatca tggatgacaa ccttttggaa ttccgtcaag actttatgga
     2341 aaaatacggt tacggtaaaa acggcagaaa tttctagaat aaacaacctc ctacagattt
     2401 gtaggaggct attttttatc cttcaaaata cagtaattct tttggtgttt ttgtaaagac
     2461 ttcaaagcca tttttggtaa cgtaaccaca atcctcgata cgaacaccaa cttttccagg
     2521 gatgtaaata cctggttcga cagagaaaca cataccttct tcgatgacca aatcattgcc
     2581 ttccataatc gatgggaatt catggacact cattcccaaa ccgtgaccga gtcggtggtt
     2641 gaagtactcg ccataacctg ctttttcaat cacagagcga gcggcatagt caatctcgcc
     2701 agcagtcaca ccaggcttaa tcatattgac agctgccatg tgagcctcca gagtcaagtt
     2761 gtaaatatct tttttaaact gatcgggttt accgacagcg acagtccgtg tcatatcaga
     2821 cgtatagccc aaagcttcca cgcctaggtc gaacaatagc agagcgttat tctcgatttt
     2881 gtttgttgag ggaataccat gtggatttgc ggcattgtca cctgttaaga ccatggtctc
     2941 aaagctcatt tttgagatgc cttgcttttt catttcaaat tcaatctgag caataatatc
     3001 agtttcagtt acatcgagtg agatgttatt gaaaccaacc tgcatagcct tatctgcaaa
     3061 ctctccagca accagcattt tttcaatttc atcacgagac ttgatcaatt tcatagtatt
     3121 aatcagaggt gtaatgtctg aaaatggttg gctaaagata gactgaagac catggtagcg
     3181 ggttaaattc aaattatcaa attctgcaca gattgctgag aaagattttt gtggcaattt
     3241 gctcttgata atctgccatg gattttctga gtccatatag ccggctacag gaaaatcaac
     3301 agtcgcaacc gcacgctcta catccaaggc agggagaaag agtagtgagt cgtggtcagg
     3361 cataacaaag agcatcatgt ggcgttcatg aggatcacta tgatagccag tcagataata
     3421 gatgctaact ggatcagaga agatggccaa gtccattttg ttggcttcta agtaggattt
     3481 aacatgatgt aattttgaca tagtggagac ctttctagca tgtagtggta aaaaaa
//
LOCUS       DBNFCK010000141         3529 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.141, whole genome shotgun sequence.
ACCESSION   DBNFCK010000141 DBNFCK010000000
VERSION     DBNFCK010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.141"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..868
                     /locus_tag="AC1PIW_001767"
     CDS             <1..868
                     /locus_tag="AC1PIW_001767"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIP6606663.1"
                     /translation="IDKINLPLPLYYSVEEAIEFSTREVVDYVFINLPSEYFDLKQLV
                     SDFELLGIDVGVDINSFGFTVLKNKKIQMLGDHSIVTFSTNFYKPSHILMKRLLDILG
                     AVVGLIICGIVSILLIPIIRRDGGPAIFAQKRVGQNGRIFTFYKFRSMFVDAEVRKKE
                     LMAQNQMQGGMFKMDNDPRITPIGHFIRKTSLDELPQFYNVLIGDMSLVGTRPPTVDE
                     FEKYTPSQKRRLSFKPGITGLWQVSGRSDITDFNEVVRLDLTYIDNWTIWSDIKILLK
                     TVKVVLLREGSK"
     gene            903..2072
                     /gene="cps2T"
                     /locus_tag="AC1PIW_001768"
                     /gene_synonym="wchF"
     CDS             903..2072
                     /gene="cps2T"
                     /locus_tag="AC1PIW_001768"
                     /gene_synonym="wchF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta 1-4 rhamnosyltransferase Cps2T"
                     /protein_id="HIP6606664.1"
                     /translation="MRTVYIIGSKGIPAKYGGFETFVEKLTEYQKDKSINYFVACTRE
                     NSAKSDITGEVFEHNGATCFNIDVPNIGSAKAILYDIMALKKSIEIAKDRNDTSPIFY
                     ILACRIGPFIYLFKKQIESIGGQLFVNPDGHEWLREKWSYPVRQYWKFSESLMLKYAD
                     LLICDSKNIEKYIHEDYRKYAPETSYIAYGTDLDKSRLSPTDSVVREWYKEKEISEND
                     YYLVVGRFVPENNYEVMIREFMKSYSRKDFVLITNVEHNSFYEKLKKETGFDKDKRIK
                     FVGTVYNQELLKYIRENAFAYFHGHEVGGTNPSLLEALSSTKLNLLLDVGFNREVGEE
                     GAKYWNKDNLHRVIDSCEQLSQEQINDMDSLSTKQVKERFSWDFIVDEYEKLFKG"
     gene            2076..3233
                     /locus_tag="AC1PIW_001769"
     CDS             2076..3233
                     /locus_tag="AC1PIW_001769"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIP6606665.1"
                     /translation="MKKILYLHAGAELYGADKVLLELIKGLDKNEFEAHVILPNDGVL
                     VPALREVGAQVEVINYPILRRKYFNPKGIFDYFISYHHYSKQIAQYAIENKVDIIHNN
                     TTAVLEGIYLKRKLKLPLLWHVHEIIVKPKFISDSINFLMGRFADKIVTVSQAVANHI
                     KQSPHIKDDQISVIYNGVDNKVYYQSDARSVRERFDIDEEALVIGMVGRVNAWKGQGD
                     FLEAVAPILEQNPKAIAFIAGSAFEGEEWRVVELEKKISQLKVSSQVRRIDYYANTTE
                     LYNMFDIFVLPSTNPDPLPTVVLEAMACGKPVVGYRHGGVCEMVKEGVNGFLVTPNSP
                     LNLSKVILQLSENINLRKKIGNNSIERQKEHFSLKSYVKNFSKVYTSLKVY"
BASE COUNT         1207 a          501 c          702 g         1119 t
ORIGIN      
        1 aatagataaa attaatttac cattaccgct ctattattct gttgaagaag ctatagagtt
       61 ttcaacaagg gaagtggtcg actacgtctt tataaattta ccaagtgaat attttgactt
      121 aaagcaatta gtttcagact ttgagttgtt aggtattgat gtaggcgttg atattaattc
      181 attcggtttt actgtgttga agaataaaaa aatccaaatg ctaggtgacc atagcatcgt
      241 cactttttcc acaaattttt ataagcctag tcacatcttg atgaaacgac ttttagatat
      301 acttggagca gtagtcgggt taattatttg tggtatagtt tctattttgt taattccaat
      361 tattcgtaga gatggtggac cagccatttt tgctcagaaa cgagttggac agaatggacg
      421 catatttaca ttctacaagt ttcgttcgat gtttgttgat gccgaggtac gtaagaaaga
      481 attaatggct caaaaccaga tgcaaggtgg gatgttcaaa atggacaacg atcctagaat
      541 tactccaatt ggacacttca tacgaaaaac aagtttagat gagttaccac aattttataa
      601 tgttctaatt ggagatatga gtctagtcgg tacccgtccg cctacagttg atgaatttga
      661 aaaatatact cctagtcaaa agagaagatt gagttttaaa ccagggatta caggtctttg
      721 gcaagtgagc ggaagaagtg atatcacaga ttttaatgaa gtcgttaggc tggacctaac
      781 atacattgat aattggacca tctggtcaga cattaagatt ttattgaaga cagtgaaagt
      841 tgtattgttg agagagggaa gtaagtaaga ctcctttaaa acaaagaata gtagtagggg
      901 atatgagaac agtttatatt attggttcaa aaggaatacc agcaaagtat ggtggtttcg
      961 agactttcgt agaaaaatta actgagtatc agaaagataa atcaattaat tattttgttg
     1021 catgtacaag agaaaattca gcaaaatcag atattacagg agaagttttt gaacataatg
     1081 gagcaacatg ttttaatatt gatgtgccaa atattggttc agcaaaagcc attctttatg
     1141 atattatggc tctcaagaaa tctattgaaa ttgccaaaga tagaaatgat acctctccaa
     1201 ttttctacat tcttgcttgt cggattggtc ctttcattta tctttttaag aagcagattg
     1261 aatcaattgg aggtcaactt ttcgtaaacc cagacggtca tgaatggcta cgtgaaaagt
     1321 ggagttatcc cgtccgacag tattggaaat tttctgagag tttgatgtta aaatacgctg
     1381 atttactaat ttgtgatagc aaaaatattg aaaaatatat tcatgaagat tatcgaaaat
     1441 atgctcctga aacatcttat attgcttatg gaacagactt agataaatca cgcctttctc
     1501 cgacagatag tgtagtacgt gagtggtata aggagaagga aatttcagaa aatgattact
     1561 atttggttgt tggacgattt gtgcctgaaa ataactatga agtaatgatt cgagagttta
     1621 tgaaatcata ttcaagaaaa gattttgttt tgataacgaa tgtagagcat aattcctttt
     1681 atgagaaatt gaaaaaagaa acagggttcg ataaagataa gcgtataaag tttgttggaa
     1741 cagtctataa tcaggagctg ttaaaatata ttcgtgaaaa tgcatttgct tattttcatg
     1801 gtcacgaggt tggaggaacg aacccatctt tacttgaagc actttcttct actaaactaa
     1861 atcttcttct agatgtgggc tttaatagag aagtagggga agaaggagcg aaatactgga
     1921 ataaagataa tcttcacaga gttattgaca gttgtgagca attatcacaa gaacaaatta
     1981 atgatatgga tagtttatca acaaaacaag tcaaagaaag attttcttgg gattttattg
     2041 ttgatgagta tgagaagttg tttaaaggat aagttatgaa aaagattcta tatctccatg
     2101 ctggagcaga attatatggg gcagataagg ttctcttgga acttataaaa ggcttagata
     2161 agaatgaatt tgaagcgcat gttatcctac ctaatgatgg agtcctagtg ccagcattaa
     2221 gagaagttgg tgcgcaagtt gaagttatta actatccaat tctacgtagg aaatatttta
     2281 atccaaaagg gatttttgac tacttcatat catatcatca ctattctaaa cagattgctc
     2341 aatatgccat agaaaataag gttgacataa ttcacaataa tactaccgct gtcttagaag
     2401 gcatttatct gaagcgaaaa ctcaaattac ctttgttgtg gcatgttcat gagattattg
     2461 tcaaacctaa attcatctct gattcgatca attttttaat ggggcgtttt gctgataaga
     2521 ttgtgacagt ttcacaggct gtggcaaacc atataaaaca atcacctcat atcaaagatg
     2581 accaaatcag tgtaatctac aatggggtag ataataaagt gtattatcag tccgatgctc
     2641 ggtctgttcg agaaagattt gacattgacg aagaggctct tgtcattggt atggtcggtc
     2701 gagtcaatgc gtggaaagga caaggagatt ttttagaagc agttgctcct atactcgaac
     2761 agaatccaaa agctatcgcc tttatagcag gaagtgcttt tgaaggagaa gagtggcgag
     2821 tagtagaatt agaaaagaag atttctcaat taaaggtctc ttctcaagtc agacgaattg
     2881 attattatgc aaataccact gaattatata atatgtttga tatttttgta cttccaagta
     2941 ctaatccaga ccctctacca acggttgtac tagaagcaat ggcatgcggt aaacctgttg
     3001 tcggttaccg acatggtggt gtttgtgaga tggtgaaaga aggtgttaac ggtttcttag
     3061 tcactccgaa ctcaccgtta aatttatcaa aagtaattct tcagttatcg gaaaatataa
     3121 atctcagaaa aaaaattggt aataattcta tagaacgtca aaaagaacat ttttcgttaa
     3181 aaagctatgt aaaaaatttt tcgaaagtct acacctccct caaagtatac tgattggctg
     3241 aagtgaatgc tttagtatag cgatttatcg tattatcatt cgataaaaca aatgttcaga
     3301 aacagttata agttatttct aaagggcacc tctataaact cccaaaattg cgaatttgga
     3361 gttacgaaag ccttgttaaa tcaacattta aattttagaa aattagtttt tagagctccc
     3421 ctaaaataga agataacaga agggagcctt caaaaacttc atttttaatt ggattgtaga
     3481 aaaactgtta aatcaatatt tagattttta ggagttcagt ttttggggg
//
LOCUS       DBNFCK010000142         3489 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.142, whole genome shotgun sequence.
ACCESSION   DBNFCK010000142 DBNFCK010000000
VERSION     DBNFCK010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.142"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(82..285)
                     /locus_tag="AC1PIW_001770"
     CDS             complement(82..285)
                     /locus_tag="AC1PIW_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HIP6606666.1"
                     /translation="MIEIHYLDAYKQERIQTFENKDAAILAFSGCLTLPDYYPVTSIT
                     QNGQALDYKGTIGDLYRYLQTLD"
     gene            complement(287..631)
                     /locus_tag="AC1PIW_001771"
     CDS             complement(287..631)
                     /locus_tag="AC1PIW_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HIP6606667.1"
                     /translation="MAFQQSVTLKDCNFTYHISPAIKKYTLRDNTFEQTKAGHYQLTR
                     LLEEIPNSNQGFLLKIIINQDLTGFKINITDKSGLHLVNIFKPESNKVIQEKFYFLMD
                     SLIDRDIFTKES"
     gene            complement(633..1748)
                     /locus_tag="AC1PIW_001772"
     CDS             complement(633..1748)
                     /locus_tag="AC1PIW_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIP6606668.1"
                     /translation="MIYLDNAATTALSPTALQRMMEVAQENYGNPSSIHQSGRKANQC
                     LRQSRQEIAQILSASPEQIIFTSGGSEADTLAIQGYALAHQSKGKHLITTAIEHHAVL
                     HTMQYLEDRFGFEVTYIQPVNQVITAQQILDALRPDTILVSVMFANNETGQLLPIKEI
                     GELLADHQAVFHVDAVQAIGKIAISPSDYGIDLLAASAHKFHGPKGMGFLYSKVHKFD
                     SLIHGGKQEQQHRAGTENLPAIAAMATALTEQYESLDTHHQHVSELRQHIIEGLSGLD
                     YYLNQAGPHLPHVLNIGFLGKLNEQLLMQLDLAGIAVSSGSACTAGVVQNSHVLEAMY
                     GKDSHRLKESIRISLSEINTKEEIDTLIIELKKILGG"
     gene            complement(1759..2736)
                     /locus_tag="AC1PIW_001773"
     CDS             complement(1759..2736)
                     /locus_tag="AC1PIW_001773"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIP6606669.1"
                     /translation="MVQPLGEHNIKLFSLNSNRDIAEKIADSAGIPLGKLSSRQFSDG
                     EIQINIEESVRGVDVYIIQSTSYPVNNHLWELLIMIDACKRASANTITAVIPYFGYAR
                     QDRTASPREPITAKLVANMLVKAGVDRVLTLDLHASQIQGFFDIPVDNLLTEPLFAAY
                     YMEKGLCGEDVVIVSPKNSGIKRARNIAEFLNAPIAIIDYAQDDSERSEGYIIGDVAG
                     KKAILVDDILNTGRTFSQASKIVQEGGATEIYAVASHGLFAGTAAQLLDEAPIKEILV
                     TDSVASKEQHPKNIAFLTASDLIADAIHRIQEHQPLSPLFKFTSPEKEN"
     gene            complement(2881..3447)
                     /locus_tag="AC1PIW_001774"
     CDS             complement(2881..3447)
                     /locus_tag="AC1PIW_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HIP6606670.1"
                     /translation="MKNHLEIEYKTLLTKSEYKRLLTDFSDVSPILQTNHYIDTPDLD
                     MKNHRFSLRIRTFEDSAELTLKIPQEVGNQEYNQVLDIQTAHDLLENFQLPAGQISDI
                     ISATEIPLDKLAVWGILTTKRYEKQTSIGLMALDENDYVGQKDYELEVEVTDADAGKI
                     LFDEFLKKKSIQFKYASSKVARTAAHMK"
BASE COUNT          997 a          619 c          757 g         1116 t
ORIGIN      
        1 acagttcttt attctattct agtccaagtt tgtgaaaaaa tcaacagatt gattttaaaa
       61 aaagattaaa caggaaactg tctaatctag tgtttgtaaa tagcgataga gatctccgat
      121 tgtccctttg taatccagag cttgtccgtt ttgtgtaatg ctggtaactg gataatagtc
      181 tgggagggtt aagcaaccgg aaaaagccag aatggctgca tccttattct caaatgtttg
      241 gatacgttct tgcttgtaag cgtcaagata gtgaatttca atcatactaa ctctcttttg
      301 taaagatgtc acggtcaatc aagctatcca tgaggaaata gaatttttct tgaataacct
      361 tgttactttc tggcttaaaa atgtttacca gatgaagacc agacttgtcg gtaatgttaa
      421 ttttaaaacc agttaggtct tgattgataa taattttcaa gaggaatcct tggtttgaat
      481 ttggaatttc ttccagaaga cgtgtaagct gatagtgacc tgctttggtt tgttcaaagg
      541 tgttatcacg gagtgtatat tttttgatag cagggctaat gtgataagta aaattacaat
      601 cttttaaggt aactgattgt tggaatgcca ttttaacctc caagtatttt ctttagttcg
      661 atgataagag tatctatctc ttcttttgta ttgatttcag ataggctgat acgaatggat
      721 tctttcagac gatgggagtc ttttccatac atagcttcta aaacatggct attttggaca
      781 acacctgccg tacatgcaga accgctagaa acagcaatac ctgccaaatc tagttgcata
      841 aggagttgtt cattaagttt accaagaaaa ccgatattaa ggacatgagg gagatgaggt
      901 ccagcctgat tgagataata gtctaagcca gacaatccct cgataatatg ttgtcgcagt
      961 tcactgacat gttgatgatg cgtatctaag gactcatatt gttctgttag ggcagtcgcc
     1021 atagcagcaa tagcagggag attctcagta cctgctcgat gttgctgttc ctgctttccg
     1081 ccgtggatca aggaatcaaa tttatgaact ttactataca ggaatcccat cccctttgga
     1141 ccatggaatt tatgggcaga agccgcgagc aagtcaattc cgtagtcaga gggagaaatt
     1201 gcaatttttc caattgcttg gacagcatct acatgaaaga cggcctgatg atcggctagg
     1261 agctctccaa tttccttgat gggaagtagc tgaccggtct cattgttagc aaacatgacg
     1321 ctgactaaaa tagtatctgg ccgaagggca tccagaattt gttgagcggt gattacttga
     1381 ttgactggtt ggatataggt tacctcgaac ccaaaccgat cctctagata ctgcatggta
     1441 tggaggacag catggtgttc gatagccgta gtaatcaggt gttttccttt ggattgatgc
     1501 gcaagtgcat agccttgaat agctaaagta tcagcttcgg agcctccaga tgtgaaaata
     1561 atttgttcgg gagaggcgga gagaatctga gcaatctctt gacgactttg tctaagacat
     1621 tgattggctt tccgaccgct ttgatgaata ctggaggggt ttccataatt ttcttgagcc
     1681 acttccatca tccgttggag tgcagtagga gatagagcag tggttgctgc attatctaaa
     1741 taaatcaaag agttactcct agttttcttt ttcaggacta gtaaatttga aaagtgggct
     1801 gagtggctga tgttcttgga tgcggtggat ggcatctgca atcaagtcac tagctgttag
     1861 gaaggcgata tttttaggat gctgttcttt gcttgcaaca gaatcggtaa ctaatatttc
     1921 tttgattggt gcttcatcta agagttgggc tgcagttcct gcaaagagac catggctggc
     1981 aactgcataa atttcagtag caccaccttc ttggacaatt tttgaagctt gtgagaaagt
     2041 gcgaccagta ttgagaatat cgtccaccaa aatagctttt ttacctgcaa catcaccgat
     2101 aatatagcct tcagaacgct cagaatcatc ttgagcgtag tcgataatcg caatcggtgc
     2161 atttagaaat tcagcgatat tacgcgcgcg tttgatacca gaatttttag gactcacaat
     2221 gacgacatct tctccgcaga gtcctttttc catgtagtag gctgcaaaaa gtggttctgt
     2281 aaggagattg tctactggaa tatcaaagaa accttgaatt tgtgaagcgt gcaaatcaag
     2341 agtcaataca cggtcgacac cagctttaac cagcatattt gcaactaatt tagctgtaat
     2401 tggttcgcgt ggagaagcgg tacggtcttg acgagcatag ccgaaataag ggatgacagc
     2461 tgtaatagtg ttggcactag cacgtttaca agcatcgatc ataatcaata attcccacaa
     2521 gtgattattt accggatagc ttgttgattg gataatgtag acgtctacgc cacgtacaga
     2581 ttcttcaatg ttaatttgaa tctcaccatc agaaaactga cgagaagaca gctttccaag
     2641 aggaattcca gcagaatcag caattttttc agcaatatca cggttgctat tgagtgagaa
     2701 taatttaatg ttatgttcac cgagtggttg taccatttta aagtaactcc ttttacacac
     2761 tgagctttta tgatgacata aaagtatttt ttacgataga tagttctatt ctatcaaaaa
     2821 aatgaaaaat tttcagtagt gaaactaaaa aatcaacctt agttggctga ttttttatag
     2881 ttatttcatg tgggcagcag ttcgtgcgac cttactacta gcatatttga attgaattga
     2941 tttcttctta aggaattcat caaaaagaat tttccctgca tctgcatcgg tcacttcgac
     3001 ttctaattcg taatcttttt gtcccacata atcattttca tcgagggcca taaggcctat
     3061 agatgtctgt ttctcatagc gttttgtcgt gaggattccc cagacagcaa gtttatcaag
     3121 tggaatttcg gttgcagaaa taatgtcaga aatttgtcct gctggtagtt gaaaattttc
     3181 cagtaaatca tgagcagttt ggatgtctag aacttggtta tattcttgat tgccgacttc
     3241 ttgtggaatt tttaaggtta gttctgctga gtcctcaaat gtacgaatac ggagtgaaaa
     3301 gcggtgattc ttcatatcta ggtctggggt atcaatgtag tggtttgttt gaagaattgg
     3361 gctcacatct gaaaagtcag taagcaggcg tttgtattcg gatttggtca gtaaggtttt
     3421 atactcaatt tcaaggtggt ttttcatgca gtaaaccctt tcaatgtgtt ataatagatt
     3481 agtctttgt
//
LOCUS       DBNFCK010000143         3454 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.143, whole genome shotgun sequence.
ACCESSION   DBNFCK010000143 DBNFCK010000000
VERSION     DBNFCK010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.143"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..313)
                     /locus_tag="AC1PIW_001775"
     CDS             complement(<1..313)
                     /locus_tag="AC1PIW_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HIP6606671.1"
                     /translation="MTVKKAKNGTWTVDISDGFHPVTKKRIRIIRKGFKTKKEALELE
                     QYIRVVELKEKRFDFLVTTDMLFDILEEEDRQNNRKISYISTQRNNYERHIKPYFINT
                     NL"
     gene            complement(303..509)
                     /locus_tag="AC1PIW_001776"
     CDS             complement(303..509)
                     /locus_tag="AC1PIW_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HIP6606672.1"
                     /translation="MIATVNKNDLVALGFSEGTSKRIIRQGKELLIARGFRVYQNKRV
                     GTIPASIATELLGFDVSLGAHHDS"
     gene            complement(570..1586)
                     /locus_tag="AC1PIW_001777"
     CDS             complement(570..1586)
                     /locus_tag="AC1PIW_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HIP6606673.1"
                     /translation="MNTVRIDYFAVTVKNIPPESVLTDILLIPLEDFTLNNWGINKYQ
                     RHYACSEIKVYFNEDRPSMGVFIELKGQGCRQYEEFLDGNENNWIALIGRLYQYSVNF
                     TRLDIAHDIFDGSLDVQRVYDYCKKGLCISKAKHFEYHEKSVLENGERVGETVAIGSR
                     GNQQWCIYNKRMEQLSKQELVEYPSWIRAELRCWQDKANIIAEQLFLKRPLSSIYFEA
                     INGHYRFVKPNATDTNRWRRKKVKWWLDYLKTENQTVLSVERTKPTLRQSEAWTEKQV
                     AKTLAKVYTAKYQAYDVQKAEDYIQGLLKEGLSRLTDNDEKDIEQYIREQNSSPLWGQ
                     KKTT"
     gene            complement(1591..2010)
                     /locus_tag="AC1PIW_001778"
     CDS             complement(1591..2010)
                     /locus_tag="AC1PIW_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606674.1"
                     /translation="MTYNMTDTTLKIGQLDLDMTQFEVPKKIVILSAKVNNRYNEVNG
                     EKIKTEEVTKITCTALDADKVKVLTEMGISTDDLKAINLEIVGNVDKVATLAQNESLL
                     NVPIELVKPKVRLAWNMARSNWAGVKLVCEDIKILGA"
     gene            complement(2346..>3454)
                     /locus_tag="AC1PIW_001779"
     CDS             complement(2346..>3454)
                     /locus_tag="AC1PIW_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HIP6606675.1"
                     /translation="VRESLFDMLIGLNLYEESDNVVTNSATLNFEILPNDIVMVEVPL
                     FGNKFLKLLKNLEEYLVPTLGLPLLSKYEFPDHVIYKLGKIEEIEQYIFNSQTLTRDF
                     FEDIPTPIVKLSNTQQFSLKQNTNLGVYGRTGTGKTIALQWYLFNALAKGCGIDTYLA
                     IVDGKGADLYALGALLQEELGKQISVGSSPTSLAKLSRQFVKIMDERFEVIKQNSSLN
                     ADAYDLGMTPNFLFIDELASIRDSCGSSKQGKELWNEILQNLGLIARKGRQAGCHLCL
                     STQDPNAENIPVELRNQISAVLYLGNIGDDRLKMAFSMCELENVPTISDRKGEALFYA
                     DGLNSVEPVLTIVPFVDVKTKQDFLQVVRNILPN"
BASE COUNT          991 a          732 c          563 g         1168 t
ORIGIN      
        1 ttaagtttgt atttatgaag taaggtttga tatgtcgctc atagttgttt ctttgtgtgc
       61 tgatataact gatttttctg ttattttgtc ggtcttcttc ctcaagtata tcgaagagca
      121 tatcggttgt taccaaaaaa tcaaaacgtt tttcttttaa ctcaacaaca cggatatatt
      181 gttctaactc taaagcttct ttcttggtct tgaaaccttt acgaatgatt cgtatccgct
      241 ttttggtaac tgggtggaag ccgtcagaaa tatcaacagt ccaagttcca ttttttgctt
      301 tcttaactgt catgatgagc acctaaagaa acgtcaaaac caagtaattc agtagcgata
      361 gaagcaggga tagttccgac acgcttattt tgataaaccc taaaccctct tgctattaga
      421 agctctttcc cctgtctgat gatacgttta gaagttcctt cagagaaacc aagggcaact
      481 aggtcattct tatttacggt tgctatcatt gaacactttc ctttcttttg ttataatcta
      541 gttagttaat ttttagaggc gacttgtttt caagtcgtct ttttttgtcc ccataaggga
      601 gaactattct gctctcgaat atattgctct atatcctttt catcattatc agttagcctt
      661 gacaagccct cttttagtag accttgaatg tagtcctctg ccttttgaac atcataggct
      721 tgatattttg ctgtatagac ttttgccaat gtcttcgcaa cttgtttctc tgtccaagcc
      781 tctgactgcc gtaatgtagg cttagttctc tcaacgctca aaacagtctg attttcggtc
      841 tttagatagt ctagccacca cttgaccttt tttcttctcc aacgattagt atctgtcgca
      901 tttggtttca caaaacgata gtgaccattg atagcttcaa aatagataga tgacagagga
      961 cgctttagaa acaattgctc tgcaataata ttcgccttat cctgccaaca tctaagttcc
     1021 gcacgaatcc atgagggata ttctacaagc tcttgcttgc taagttgttc catacgtttg
     1081 ttgtagatac accattgttg attacctctg gaacctatag caaccgtctc tccaactcgt
     1141 tctccatttt ctaagacaga tttctcatga tactcaaaat gctttgcttt cgagatacat
     1201 aaacctttct tgcaatagtc atatactcgc tgaacatcta aactaccgtc aaatatgtcg
     1261 tgagcaatat ctaagcgagt aaaattaacc gaatactgat acaaccgacc tatcaaagct
     1321 atccaattat tttcgttacc gtccaaaaat tcttcatatt ggcgacaacc ttgccctttt
     1381 aattcgataa aaacccccat tgaaggtcta tcctcattaa agtacacttt aatctcagaa
     1441 caagcataat gtcgctggta cttgtttatt ccccaattat ttaatgtgaa atcttctaaa
     1501 ggaattaaaa gaatatcagt taaaacactt tctggaggaa tatttttaac ggttactgca
     1561 aaataatcaa ttctgactgt attcataggg ttatgctcct agaattttaa tgtcctcaca
     1621 tactaattta acacctgccc agttacttcg tgccatgttc caagctaaac gaactttcgg
     1681 cttaaccaac tcaatcggaa cattcaaaag tgattcattt tgagcgagcg tagcaacctt
     1741 atctacatta cctacaattt ctaggttgat agctttcaag tcatcagtag aaattcccat
     1801 ttcagttaga actttcactt tatcggcatc aagggcagta caagtgattt tcgtaacttc
     1861 ttcagttttg attttctcgc cattaacctc gttataccta ttatttactt tagcagatag
     1921 aataacaatt tttttaggca cttcaaactg tgtcatatcc aaatcaagtt gcccaatctt
     1981 aagggtagta tctgtcatat tgtaagtcat aataattact ccattttcta ttcaaaaagt
     2041 cgtatcctac acgaaaagcg actaaatccc ttctcccaca agggtttcaa tgacttcggt
     2101 ataaccaaca agccgtaacc ccttacgtat caagggttta gagtacattt catgtatgtg
     2161 tgcaccccct attagagtac atttcatgta tgtgtgcacc ccctattagt aatgggggta
     2221 tttttctgac ctcctgcaaa gcagtcggtt ggctctttct cctagcgaaa agaggaacgc
     2281 tccgctcgat tctgccccct gggcaatcgt cccccctatt tcgctagtcc gaaagaccgt
     2341 cattattagt ttggcagtat atttctaact acctgtaaaa aatcttgttt tgttttaaca
     2401 tcgacgaacg ggacgatagt tagtacaggc tcaacagaat ttaaaccgtc agcataaaag
     2461 agtgcttcgc ctttcctgtc tgaaattgta ggaacatttt ccagttcaca catactaaaa
     2521 gccattttca atcggtcgtc ccctatattt ccgaggtata aaactgctga tatttggttt
     2581 ctaagttcaa caggaatatt ctctgcattt gggtcttggg tactaaggca gaggtggcaa
     2641 cctgcctgcc gtcctttgcg tgcaatcaag cctaagtttt gcagaatttc attccatagc
     2701 tctttacctt gtttactaga accacaacta tcacgaatag aagcaagctc atcaataaac
     2761 aaaaaattag gggtcattcc taaatcatag gcatcagcat ttagcgaact gttctgcttg
     2821 ataacctcaa aacgttcatc cataattttt acaaactgtc ttgatagctt tgctagtgaa
     2881 gtaggagaag agcctactga aatttgtttt cccagttctt cttggaggag tgccccaaga
     2941 gcatataagt cagctccttt accatcaacg atagctagat aagtatcaat accgcaccct
     3001 ttggcaagtg cattaaataa gtaccattgt agagctattg tcttccctgt acccgtcctt
     3061 ccatatactc ctaaattagt attctgtttt agagaaaatt gctgagtatt ggatagttta
     3121 actattggcg taggtatgtc ttcaaaaaaa tctctagtca atgtttggct attgaatata
     3181 tactgctcaa tttcttctat ctttccaagt ttataaataa catggtcagg aaattcatat
     3241 ttggatagta gaggtagacc caaggttgga acaagatatt cttcaagatt ttttagaagt
     3301 tttaaaaact tattcccaaa caatggtact tcgaccatta caatatcatt gggcaagatt
     3361 tcaaagttta atgttgcact attggttact acattatcac tttcttcgta taaatttagg
     3421 ccaatcagca tatcaaatag agattctcta acat
//
LOCUS       DBNFCK010000144         3446 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.144, whole genome shotgun sequence.
ACCESSION   DBNFCK010000144 DBNFCK010000000
VERSION     DBNFCK010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.144"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            38..727
                     /locus_tag="AC1PIW_001780"
     CDS             38..727
                     /locus_tag="AC1PIW_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIP6606676.1"
                     /translation="MNFSFLPEYWAYFNYGAIVTLIIAFFSVFFGSILGVLLAFAQRS
                     KYKVLAWTANIYVWIFRGTPMIVQIMIAFAMTHFVAPTFQLGILTVDLTRLIPGIIVI
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKQAMRYVIMPQAIKNILPALGNEF
                     VTIVKDSSLLSTIGVMELWNGAQTVQSTSYIPLTPLVFAASYYLIMTTVLTVIIQSFE
                     KKLNKGGQFHV"
     gene            720..1454
                     /locus_tag="AC1PIW_001781"
     CDS             720..1454
                     /locus_tag="AC1PIW_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019805208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIP6606677.1"
                     /translation="MSNAIISIKDLHKYFGKNEVLKGIDLDIQQGQVVVIIGPSGSGK
                     STFLRTMNLLEVPTKGTVTFEGVDITDKSNDIFKMREKMGMVFQQFNLFPNMTVLDNI
                     TLSPIKTKGIAKDEAEKKAKELLEKVGLPDKANAYPQSLSGGQQQRIAIARGLAMDPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMDGGVI
                     VEDGTPEEVFEHTKEERTKDFLSKVL"
     gene            1584..2432
                     /locus_tag="AC1PIW_001782"
     CDS             1584..2432
                     /locus_tag="AC1PIW_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HIP6606678.1"
                     /translation="MTVIDGKALGVKLQAALAEKTARLKEEKGLVPGLVVILVGENPA
                     SQVYVRNKERSALAAGFKSEVVRVPDTISESDLLDLIERYNQDDEWHGILVQLPLPAH
                     ISEEKVLLAIDPDKDVDGFHPTNMGKFWSGHPVMIPSTPAGIMEMFKEYQIELEGKSA
                     LVIGRSNIVGKPMAQLLLDADATVTIAHSRTKNLPDLARQADILVVAIGRGHFVTKEF
                     VKLGAVVIDVGMNRDENGKLIGDVKYDEVSEVASYITPVPGGVGPMTITMLMEQTYEA
                     CVRSAK"
BASE COUNT          995 a          549 c          779 g         1123 t
ORIGIN      
        1 ataatagact ctaaatgaca gaattggagt aagatgaatg aatttttctt ttttgcctga
       61 gtattgggct tattttaatt atggggcaat tgttaccctc attattgcat ttttctcagt
      121 attttttggt agtattttag gtgtgttgct tgcctttgct cagcgtagta agtataaagt
      181 tttagcttgg actgccaata tctatgtctg gattttccgt gggacaccga tgattgtcca
      241 gattatgatt gcttttgcga tgacacattt tgtagcacca acttttcagc taggaatttt
      301 gacggttgat ttgacgcgct tgattccagg tatcattgtt atttcgatga actcgggtgc
      361 ctatgtatct gaaactgttc gtgcaggtat taatgctgta ccaaaaggac agctggaagc
      421 agcttattcg cttggtatta gaccaaagca ggcgatgcgt tacgttatca tgccacaggc
      481 tatcaagaat attcttccag ccttgggaaa tgaatttgtg actattgtta aggatagttc
      541 acttttgtca accatcggtg tgatggaatt atggaacggt gctcagacag ttcaatccac
      601 ttcttatatt cctttaacac cacttgtatt tgcagcgagc tattatctaa ttatgacaac
      661 ggtgctaaca gtgattattc agtcgtttga gaaaaagttg aacaagggag ggcagttcca
      721 tgtctaatgc gattatttct atcaaggatt tacataagta cttcggaaag aatgaggttc
      781 taaaaggaat tgatttagat attcaacaag gtcaggtggt cgttattatc ggtccatcag
      841 ggtcagggaa atcgactttc ttacgtacaa tgaacctctt agaagtgcca accaagggaa
      901 ctgttacatt tgaaggtgtt gatattactg acaagtcaaa tgatattttc aagatgcgtg
      961 aaaagatggg aatggttttt caacagttca atctttttcc gaatatgacg gtattagata
     1021 acattacttt atcacctatt aagacaaagg gaattgcaaa ggatgaggct gagaagaagg
     1081 ctaaggaatt acttgaaaag gtaggattgc cagataaggc aaatgcctat ccacaaagtc
     1141 tttcaggtgg tcagcaacaa cggatcgcta ttgcacgtgg tctggccatg gacccagatg
     1201 tcctactttt tgatgaaccg acctctgcac tagaccctga aatggttggt gaagttcttg
     1261 ctgtaatgca ggatttggcc aagtcaggga tgaccatggt tatcgtgact catgagatgg
     1321 gatttgcgcg tgaggtagct gacagggtta tctttatgga tggcggtgtc atcgtggagg
     1381 atggaacgcc tgaagaagtc tttgaacata ccaaggaaga acggaccaag gatttcttgt
     1441 ctaaggtctt gtaataaatg aacaaataag tacgactccg atattgttcg gagttttttg
     1501 ctatatgcta tattctactt tgtacaagta tagtcagtcg gagaatttta gggtataata
     1561 gaaaaaacaa aaggagtgta ggtatgacag tcattgatgg gaaagcacta ggtgtaaaat
     1621 tgcaggctgc tttggcggag aaaactgctc gattaaaaga agaaaagggg ttggtaccag
     1681 gtcttgtagt tattttagtt ggcgaaaatc ctgctagtca ggtttatgta cggaataagg
     1741 agcggtcagc tctagctgct ggattcaaga gtgaggttgt tcgcgttcca gatacgattt
     1801 ctgagtcaga cttgttggat ttgattgagc gctataatca ggatgatgaa tggcatggta
     1861 ttttggtgca attgccctta ccagcccaca tcagcgagga gaaggtcttg ttggccattg
     1921 acccagataa ggatgtggat ggttttcatc cgactaacat ggggaaattc tggtcaggtc
     1981 atcctgttat gattccatct accccagcag gaattatgga aatgttcaaa gaatatcaga
     2041 ttgagctgga agggaagtct gctttagtta ttggtcgttc taatattgtt ggtaaaccga
     2101 tggcacagct gttattggat gccgatgcta ctgtaacgat tgcgcattct cgcaccaaaa
     2161 acttgccaga tcttgctcgt caggccgata ttcttgttgt tgccattggt cgtggtcatt
     2221 ttgtgaccaa ggaatttgtt aagctaggtg ccgtggtgat tgatgttggt atgaatcgtg
     2281 atgaaaatgg caagctaatc ggtgatgtca aatacgatga ggtatcagaa gtggcaagtt
     2341 atattacccc ggttccaggt ggtgttggtc cgatgactat taccatgttg atggaacaga
     2401 cctatgaagc ttgtgtcaga agtgcaaaat aagatagaga gtttaataaa aataaatata
     2461 tccatgcctg ctttggtaaa gccctgttct tagaacctgt atttacaagc gaagtactca
     2521 tattaagaga cagatttagc taatcaaggt aatttttttg ggggatattg attgtatttt
     2581 gaaaaaaact aatgatgagt agcttaaaaa ctagccttag aatagaagcg ttgaactgtg
     2641 aatacaggtt cttaaattat gcttgtagtt ttggtagcta acagtaatga gtgcagtccc
     2701 cgtatgaata agtctatttt taaacgactc tacttatttt attagtcata ttcaataaat
     2761 cagttttaag acggatgtga cttttagtaa tattggttat actataccca atcctaaata
     2821 tttttcataa tctcctgaaa agcctgatcg tgtgatcagg tttttttgta tgcaaaaatg
     2881 gatctgtaca aaaaaacagt actatttctc aaagtttgaa caacatccca gtgtagtaaa
     2941 aacgtttgga gacagattgt ttcagctcaa caactgggaa taaagattta ttgccgaact
     3001 cttttatact cttcgaaaat caaaatcaga cgttgttgac ttgatttgat gaacttcagt
     3061 tctatctgca tctgcgtcgc ctagtctgat tttcattgag tattatctgt ttagttcttt
     3121 ctctctttca ttttgtgttt ataatgtttt aggtgattgt gggacgtttt cagtgaaact
     3181 gctaatggaa cggttttact cctagcctta tctgagtaat aataggataa aactcatata
     3241 cccacataag agtgatagag gtttgtgaat agtatttagt tagaaacatt tgttatcaaa
     3301 tacaggttct acgttatatt taaagaactt ctctatcatt cgttaaatat ttcttctccg
     3361 atttatcgct agataggagc agtgtatgcg tttctaaaaa tctaaagttg gaggatgttc
     3421 aattttaaat aagttacaat ctgcaa
//
LOCUS       DBNFCK010000145         3359 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.145, whole genome shotgun sequence.
ACCESSION   DBNFCK010000145 DBNFCK010000000
VERSION     DBNFCK010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.145"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..3141
                     /locus_tag="AC1PIW_001783"
     CDS             <1..3141
                     /locus_tag="AC1PIW_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HIP6606679.1"
                     /translation="TIDKEQIITIKTPKNSKARYVRFRGTRVDDTPLRYSEIQIMGRG
                     KSYDESAPEYVPPKSEYDTLVWSDEFNGDKIDESKWQIIDGMWNHAAIYNRKAVSIKK
                     DGDKSYLSIRSTNHKDKKTLVEAVGHDRYDDKELPDKVTWSSGRLESKDKYSFQFGRM
                     AVRAKVNDSKGIWPAIWMLAQDETGHDEIDVLEYLGQNPWYAWTTNHFGILAKNKKSD
                     GSAYKNYEAWSQEFHVYEVEWTPDVIKWYIDGKFVFQTDRGKDRDGMHTRPMFPILET
                     QVGDGWVGDVDYKRNMTKQDSEYLVDWVRIYQKADQSKVRFDNLDDAKPNDYVIKPQQ
                     KLGHLVTVSNGKAAHENKNNFYYGGQPRYETSRLYADGDGENALVYKIANPEALHLTT
                     YYKTLEDYSVYNKEAGANEGKSVRKHLINAKDGEIDFTVYSSVDGKSWNKENVTVVDN
                     FVEATPAYARTTFDIRNIRKGTKFVKIVFPTVKGISYKLEDGTEKGLQASNVQLAKVT
                     FVATHPEAEPEKPIVDQEKLALSKVFSLDAGRKYFSVNQIKEIIDLISSYGYTDMNLL
                     LGNDGLRFLLDDMNLTVGDKVYDSEAVRKAIINGNKHYYDDPNGTTLSQSEMDDILAY
                     AKEKNISIIPTINSPGHMDAIVEAMEELGIKSPKFSYNGKTSARTVDLKNDQAVAFTK
                     ALIDKYASYFAGKSKIFNIGLDEYANDATNASGWHILQTQGDYGKFIQYANDLAAIVK
                     KHGLEPMAFNDGIYYNHDTSSGTFDKDIIASFWTGGWGGYDVASSKFLHEKGHKILNT
                     NDAWYYVIGREAEGLGWYNLDQGLRGTKSTRFDQVPKSENTKVPIIGSMVAVWADDPS
                     KPYSYDSIKKLLHSFTENNSDYFLAEYRDLREEVGKIPTDLNSYTPESVATLKEVLNK
                     IDWKLSRNNQAFVDGYLAEVKAAREALVPVTAKGDSVKAEPLPEGKLPLITAKGKSVQ
                     AEPRPAFKGYLTSNYGPSVTPLSLVSKPSSEQSNSKQAASQKKELPNTGVVDGLGFSL
                     LGMIGLAFASRRRKKE"
BASE COUNT         1105 a          567 c          729 g          958 t
ORIGIN      
        1 accattgaca aggaacagat tataacaatt aaaactccga agaactctaa agcgcgttat
       61 gttcgcttta gaggtacgcg cgtagatgat actcctcttc gatattcaga aattcagatt
      121 atgggacgag gaaaatctta tgacgaatct gctccagaat atgttccacc aaaatctgaa
      181 tacgatacat tagtctggtc tgatgaattt aatggcgaca agattgatga aagtaaatgg
      241 caaattattg atggcatgtg gaatcatgcg gctatataca atcgtaaagc agtttctatc
      301 aaaaaagatg gcgacaagag ctatttgtct atccgtagca cgaaccataa ggataaaaaa
      361 actttggttg aagcagttgg gcacgatcgg tatgatgata aagaattacc agataaagta
      421 acatggtctt ctggtcgcct agaatctaaa gataagtatt ctttccaatt tggtcgaatg
      481 gctgttcgag ctaaggtaaa tgactctaag ggaatctggc ctgctatttg gatgttggca
      541 caggatgaga ccgggcatga cgaaattgac gttttggaat accttggtca aaatccatgg
      601 tatgcatgga caactaacca ttttggtatt ttggcaaaaa ataaaaaatc tgatggctca
      661 gcttataaga attacgaagc ttggagtcag gaattccatg tctacgaagt cgaatggact
      721 ccagatgtca tcaaatggta tattgatgga aaatttgttt ttcaaacaga ccgtggtaag
      781 gatcgggatg gtatgcacac ccgccctatg ttcccgatac tggaaaccca agttggtgat
      841 ggctgggttg gtgatgttga ttacaaacgc aacatgacta aacaggatag tgagtacttg
      901 gttgattggg ttcgaattta tcaaaaagca gatcaaagta aggtacgttt tgataatcta
      961 gatgatgcca agcctaatga ttatgttatt aagccgcagc aaaaacttgg tcacctcgta
     1021 acagtctcta acgggaaagc agctcatgaa aacaaaaata atttctacta tggaggtcaa
     1081 ccacgttacg aaacaagcag gctctatgct gatggtgatg gcgaaaatgc actagtctac
     1141 aagattgcta atccagaggc tttgcactta acgacttatt acaaaacact tgaagactat
     1201 tctgtctata ataaagaagc aggggctaat gaaggaaaat ctgttcgcaa acatttgata
     1261 aatgcaaaag acggtgaaat tgacttcact gtgtattcat ctgttgacgg caaaagctgg
     1321 aataaagaga atgttactgt agtggataat tttgtagaag caaccccagc ttatgcacgc
     1381 actacatttg atattcgcaa cattcgaaaa ggtactaaat ttgtgaagat cgtctttcca
     1441 acggttaaag gtatttccta taagttggaa gatggaacag agaaaggctt gcaagccagt
     1501 aatgtccagt tagcaaaagt aacttttgta gctactcatc ctgaagcaga accagaaaaa
     1561 cctattgttg atcaagaaaa actagccttg tccaaagttt tttcactcga tgccggtcgc
     1621 aagtatttct ctgtaaatca gataaaggag attattgatt taatttcttc ttatggttac
     1681 acggatatga acctcttatt gggaaatgat ggtctgagat ttttattaga tgatatgaat
     1741 ttgaccgttg gagataaggt gtatgacagt gaagcagtta gaaaagcaat aataaatggt
     1801 aataagcatt attatgacga tccaaacggt acaaccttaa gtcagagtga gatggatgat
     1861 attcttgctt atgctaagga aaaaaatatt tccattattc caactatcaa tagcccaggt
     1921 catatggatg cgattgtaga agcaatggaa gaattgggga taaaatcgcc aaaatttagt
     1981 tataatggca aaacatctgc acgtacagtt gatttaaaaa atgaccaagc agttgctttt
     2041 actaaagcct taattgataa atatgctagt tactttgcag gcaaatctaa aattttcaat
     2101 attggcttag atgagtatgc taacgatgct acaaatgctt ctggatggca cattcttcag
     2161 acacaaggtg attatggtaa gtttattcaa tatgccaatg atttagcagc tatcgtcaag
     2221 aagcatggtt tagagccaat ggctttcaat gatggtatct attataacca cgatactagt
     2281 tcgggtactt ttgataaaga cattattgct tctttctgga caggaggttg gggaggatat
     2341 gatgttgctt cctcaaaatt ccttcatgaa aaagggcata aaattcttaa tacaaatgat
     2401 gcttggtact atgttattgg acgtgaagca gaaggtttgg gatggtataa ccttgatcaa
     2461 ggtcttcgag gaactaaatc tacaaggttt gatcaagtac cgaaatctga gaatactaag
     2521 gtacccatta ttggtagtat ggttgcggta tgggcagatg acccaagcaa gccttacagc
     2581 tatgactcaa ttaaaaaact tttgcacagt tttacggaaa acaattctga ttattttttg
     2641 gcagagtacc gagatttgcg agaagaagtt gggaagattc ctactgattt aaattcctat
     2701 acaccagaga gcgtggcgac tttaaaagag gtcttgaaca agattgattg gaaactaagt
     2761 agaaataacc aagcgtttgt tgatggttac ttggcagaag taaaagcagc gcgtgaagcg
     2821 ttagttcctg tgacagcaaa gggagacagc gtcaaagcgg agcccttacc agaaggcaag
     2881 ctacctctga ttactgctaa aggaaaaagt gtccaagcag aaccacgccc tgcctttaag
     2941 ggatacctga catcaaacta tggaccatct gtcacacctt tatccctcgt atcaaaacca
     3001 agcagtgagc agtcaaattc caagcaagca gcttctcaga agaaagaatt gccaaataca
     3061 ggagtagtag atggtcttgg attctctctc cttgggatga ttggtctggc atttgctagt
     3121 cgccggcgga agaaggagta atgtagccac ttgaattaag gttcagggct ttctaattgt
     3181 aattatatag ttatctagtt tttctttcac tcgctttgct gtacccaagt atagcatgta
     3241 gcttattgcc ttgtacaaaa agtaaactag aaagctatga ataacaggat aattttttat
     3301 cggctactaa aaatcccccg taataaatct tattacggga gattttcgtt tatgagtat
//
LOCUS       DBNFCK010000146         3304 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.146, whole genome shotgun sequence.
ACCESSION   DBNFCK010000146 DBNFCK010000000
VERSION     DBNFCK010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.146"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            166..348
                     /gene="rpmF"
                     /locus_tag="AC1PIW_001784"
     CDS             166..348
                     /gene="rpmF"
                     /locus_tag="AC1PIW_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIP6606680.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVAAPTVKFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            364..513
                     /gene="rpmG"
                     /locus_tag="AC1PIW_001785"
     CDS             364..513
                     /gene="rpmG"
                     /locus_tag="AC1PIW_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIP6606681.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVI
                     FTEVK"
     gene            complement(984..2174)
                     /locus_tag="AC1PIW_001786"
     CDS             complement(984..2174)
                     /locus_tag="AC1PIW_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIP6606682.1"
                     /translation="MTNREMQTFPVLAVVKNFKERIFFSRDEITSAAGLRLKVSESAK
                     PNNEGITIIGDSSPESDRTPEPRGDRTPDIDVPKAEVPKVEQPKPKAPEVQLPAPKAP
                     ESVPPKVMPPAPKVPDVKTPKVEYPDTDAPEIESPKAPETERPEVLPPTPKVPEVEVP
                     KVIPPTSKVPEAELPKVEQPKPKVPEVHLPAPKSPETEPPKVMSPAPKVPDVKTPKEA
                     PLPPKTPGVEVPKVIPPTPKAPEVEVPKVTPPTSKVPEVETPKVEQPKPKVPEVHLPA
                     PKSPEVEVPKVTPPTSKIPDVKTPKSPEVAHPEVEKPAGIQPEKGQPEVNKPNIIHSE
                     VLQSEVKKTESGVSMAPKVGIQKETLPNTGETSNTLVWLGGALATLASSLYLFKQHRQ
                     REEE"
     gene            complement(2304..3023)
                     /locus_tag="AC1PIW_001787"
     CDS             complement(2304..3023)
                     /locus_tag="AC1PIW_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HIP6606683.1"
                     /translation="MSKQKVVTNLLLSTAVLGGFLICHAPSVSAETGNLQEIPTESAT
                     VSSENQTATAVDGISPTDNQLPTSEEGDKQAPEEKLVSSDSTREESATQVATDPDAPS
                     QSLSVSGAEISTADSNIQQAEEKLIQESNYYRFDTHQGEQIRKAVSIEKISADNDEIK
                     WKVTFDRKNWTFSGEGSGYYFILPKGLQLTKIIDSQSEEDIFNKFPKDVNSEANSGGQ
                     PYRFFSREKNANNDERSFESQ"
BASE COUNT          887 a          695 c          621 g         1101 t
ORIGIN      
        1 ttaccataaa tgctctgcta ttttccaaaa aagtgaaaaa aatctgtcaa gtaactttac
       61 tttacaaaaa acttttgtta tactgttaaa gttggcgaaa gtcatacgca ctatttttag
      121 atttaaaaag ggcttgaccc tgacatttaa tggaggaaga aagaaatggc agtacctgca
      181 cgtcgcactt caaaagcgaa gaaaaacaaa cgtcgtactc actataaagt agcagctcca
      241 actgtgaaat ttgatgaaac tactggagat tactcacgtt cacaccgtgt atctttgaaa
      301 ggatactaca aaggacgtaa aattgccaag gctgcttcag ctgaataata gaagggagat
      361 accatgcgcg taaatattac acttgaacac aaagaatctg gtgagcgttt gtaccttact
      421 tcaaaaaaca aacgtaacac tccagaccgt cttcaattga aaaaatactc accaaaattg
      481 cgtaagcacg tgatttttac tgaggtaaaa taagacaaca tacagaaagc ctatgaaatc
      541 aacgtttcaa aggctttttt cttttgtttt tgacaaaata actgaatttt aactgattaa
      601 aataaatcaa atgataattt ctgctaaatc tagcatagcc tgttcaatct tacgttttcc
      661 tttttgtatt acatgaagat agattttcac tatctcctta ccatcctcag aatgtcctac
      721 aaattctcga ataacattca aactagtatt gataaaacaa aatatataac caactccttc
      781 atatcattgc aaacgataaa tttcaatctt gtctttgtgt cttgacctta caagaaaagc
      841 acctcaaaaa ttagatttca cctctaactt ttggggtgca gttcagattt tggaacgttt
      901 tctatttaca actgttctaa attttcttag tccatggtag ataagcctta ttggcgttcc
      961 aaaaatatag cattagcgta aaatcattcc tcttctcttt gcctgtgttg tttgaaaaga
     1021 tacaaacttg atgcaagggt agctagagca ccacctagcc atactagggt gttgctcgtt
     1081 tcacccgtat ttggtaaagt ctctttttgt attcctacct ttggagccat tgaaactcca
     1141 gattccgttt tcttaacttc ggattgaagc acctccgaat ggataatatt gggtttatta
     1201 acctcaggtt gacccttttc tggttggatt ccggcgggtt tctcaacttc tgggtgcgca
     1261 acttccggag attttggcgt cttaacgtct ggaatttttg aggttggtgg tgttactttc
     1321 ggaacctcaa cttctggaga cttaggagca ggaaggtgaa cttctggaac cttcggtttt
     1381 ggctgctcaa ctttcggtgt ctcaacttct ggaactttcg aggtcggtgg tgttactttc
     1441 ggaacctcaa cttctggggc ttttggggtc ggtggaatta ctttcggaac ctcaacgccc
     1501 ggagtttttg gtggcagagg cgcctctttt ggcgtcttaa cgtctggaac tttgggggct
     1561 ggggacatta ctttgggtgg ctcagtttcc ggagacttag gagcaggaag gtgaacttct
     1621 ggaaccttcg gttttggctg ctcaactttc ggtagctcag cttctggaac ttttgaggtc
     1681 ggtggaatta ctttcggaac ctcaacttct gggacttttg gcgtcggtgg taagacttct
     1741 ggtcgttcag tttcaggagc ttttggtgac tcaatctcag gagcatctgt atcagggtac
     1801 tcaacttttg gcgtcttaac gtctggaact ttgggggctg gaggcattac ttttggtggc
     1861 acagattccg gagccttagg agcaggcagt tgaacttcag gggcctttgg ttttggttgc
     1921 tcaacttttg gaacctctgc ctttggtaca tctatatccg gagtcctatc tccccgcggt
     1981 tcaggagtcc tgtcgctttc tggagatgaa tctccgataa tagtaatccc ttcattattt
     2041 ggcttagcac tttccgatac ctttaatctc aaaccagcag cagaagtaat ctcatctctg
     2101 gagaaaaata ttcgctcttt aaaattcttc accacagcta aaaccgggaa agtctgcatt
     2161 tctcgatttg tcacctgtgc cgtcagttca tatgtcgctt tttctttagc aacggaatct
     2221 cgctgtactt gtagaaaata gatgctggaa ctttcttcct tccatttttc tagctctcct
     2281 cctacacgtc caacagacca acctcactga ctttcaaaac tacgctcatc attattagca
     2341 tttttttctc tactaaaaaa gcgatacggc tgcccgccag aatttgcctc actattgaca
     2401 tccttaggga acttattgaa aatatcctct tcagactggc tatcaataat tttggttaat
     2461 tgtaagcctt ttggaagaat aaagtaatag ccactcccct ctccactaaa ggtccaattt
     2521 tttctatcaa aagttacttt ccacttaatt tcgtcattat ctgccgatat tttttctata
     2581 gatactgcct tcctaatctg ttcaccttga tgcgtgtcaa atctataata attcgattct
     2641 tgaatcagtt tctcttcggc ttgttgaata tttgaatctg ccgttgaaat ttccgcccct
     2701 gacacactta atgattgaga tggtgcatcc ggatctgtcg ctacctgcgt agcactttct
     2761 tctctcgtag aatcgctaga aactagtttt tcttctggag cctgtttatc accctcttct
     2821 gacgtaggta attggttatc cgtaggtgaa attccatcga ctgcggtcgc agtttgattt
     2881 tctgagctaa ccgtagcaga ttcagtaggt atttcttgaa gattgcctgt ttctgcgctt
     2941 acagatggtg cgtgacatat tagaaaacca cctagtacag ctgtactaag caatagattt
     3001 gtcacaactt tttgtttgct cataatgtgc tcctttactc tatttacttc aatttactaa
     3061 gaacctgtat ttacaggtta gcactcccat ctaaggcatt tttaagctac tcatcgttcg
     3121 ttttttcaaa atacagccag cattccctca aaaaattaac ttgattagcg aaaaactatc
     3181 tcttacataa aagctcttta cttgtaagta caggttctaa aatatggcga ttagattttt
     3241 attccaatct agtattttca aaaattgata ttattgtttt agcgttagta taacatccta
     3301 aact
//
LOCUS       DBNFCK010000147         3250 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.147, whole genome shotgun sequence.
ACCESSION   DBNFCK010000147 DBNFCK010000000
VERSION     DBNFCK010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.147"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..768
                     /gene="dprA"
                     /locus_tag="AC1PIW_001788"
     CDS             <1..768
                     /gene="dprA"
                     /locus_tag="AC1PIW_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIP6606684.1"
                     /translation="QEKHGKKLTLRNVAVVSECKNPVLFMENYKNLDTKACREEFNKY
                     PSFSILDDIYPLALKQIYNPPVLLFYQGDLELLEKLKLAVVGTRNASKNGIQSVQKII
                     KELSNRFVIVSGLARGIDTAAHIASLKNGGQTIAVIGTGLDNIYPKENKDLQTYIGKH
                     HLVLTEYEAGAQPLKYHFPERNRIIAGLVEGLVVCEAKMRSGSLITCERALEEGREVY
                     AIPGSIMDGKSDGCHHLIQEGAKCVTSGLDIISEYQF"
     gene            861..2954
                     /gene="topA"
                     /locus_tag="AC1PIW_001789"
     CDS             861..2954
                     /gene="topA"
                     /locus_tag="AC1PIW_001789"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HIP6606685.1"
                     /translation="MATKTVSKKKTTTKKNLVIVESPAKAKTIEKYLGKNYKVVASVG
                     HIRDLKKSSMSIDFDNNYAPEYINIRGKGPLINSLKKEAKNAKQVFLASDPDREGEAI
                     SWHLAHILGLDANDKNRVVFNEITKDAVKNAFQEPRAINHDLVDAQQARRVLDRIVGY
                     SISPILWKKVKKGLSAGRVQSVALKLIIDRENEISNFKPEEYWTIDATFKKGSKKFQA
                     SFYGIDGKKTKIENNEQVKEILARLDGDDFLVEQVERKERKRNAPLPYTTSTMQMDAA
                     KKINFRTRKTMMVAQQLYEGVSLGTGGTQGLITYMRTDSTRISPVAQAQAADFITERF
                     GATYSKHGNKVKNASGAQDAHEAIRPSNVNLTPESIAKYLDKDQLRLYTLIWNRFVAS
                     QMAAAVFDTMSVRLGQNGVIFAANGSQVKFDGYMAVYNDSDKNKMLPDMEQGDTVVRT
                     STNPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVKLAAKRFEPT
                     ELGEIVNKLIVEFFPDIVNVKFTADMEQKLDDVEIGKEQWQKVIDGFYQPFKVELFKA
                     EEQMEKIQIKDEPAGFDCDVCGHEMVIKLGRFGKFYACSNFPDCRNTKQITKEIGVTC
                     PVCQQGQVIERKSKRNRLFYGCDRYPECEFTSWDKPVGRACPKCDHYLVEKKVRGGGK
                     QVVCPNGDYEEDKIK"
BASE COUNT         1070 a          576 c          706 g          898 t
ORIGIN      
        1 caagaaaaac acggtaaaaa attgaccttg cgaaatgtcg ctgtagtgtc tgaatgtaaa
       61 aatccagtgc tatttatgga aaattataag aatctagata caaaagcctg tcgcgaggaa
      121 tttaataagt atccatcatt ttcaatttta gatgacatct atcctttggc gctcaaacag
      181 atttacaatc cacctgtact tttgttttat caaggggatt tagaattgct agaaaaatta
      241 aagctggcag ttgttggtac acgaaatgcc agtaaaaatg gtatccaatc tgttcagaaa
      301 attatcaagg aattgagcaa tcgttttgtc attgttagtg gtttggcaag aggaattgat
      361 acagctgccc acatcgccag tttgaaaaat ggaggccaaa ccattgctgt aatcggaaca
      421 ggtttagata atatttatcc aaaagaaaat aaagatttgc agacctatat tggaaaacac
      481 catttggtgc tgacagagta tgaagctggt gctcagccac tcaaatacca ttttccagaa
      541 cgcaatcgca ttatagctgg tttggtggaa ggattggtag tctgcgaggc aaaaatgcgg
      601 tctggcagct tgattacttg tgaacgtgcc ttggaagaag gaagagaagt ctatgctatt
      661 ccaggttcca ttatggacgg aaaatcagat ggttgtcatc atttgattca agagggagca
      721 aagtgcgtta catcaggatt ggacatcatt tctgagtatc aattttaaaa caagtcttcc
      781 tataggaaaa taaatatctt gacactgact gaaaaatagt atattctttg tgaggttaaa
      841 aatatttgta aggtataaga atggctacaa aaacagttag taagaaaaaa acaacaacca
      901 agaaaaatct cgttattgta gagtcacctg ctaaggcaaa aacaatcgaa aaatatcttg
      961 gtaaaaatta taaggtcgtt gcttcagtcg ggcatattcg agatttgaag aaatctagta
     1021 tgtctattga ctttgataac aactatgcac ctgagtacat caacatacgt ggaaaaggac
     1081 cactcatcaa ttctttgaaa aaagaagcaa aaaatgctaa gcaagtcttt ctcgcgagtg
     1141 acccggaccg cgaaggagaa gcaatttctt ggcatttggc tcacattttg ggattggacg
     1201 caaacgataa aaaccgtgtt gtctttaatg aaattacaaa agatgcagta aaaaatgctt
     1261 ttcaggagcc gcgcgctatc aatcatgact tggtagatgc ccagcaagct cgtcgcgtct
     1321 tagaccgtat tgtagggtac tccatatcac ctattttatg gaagaaagtc aaaaaaggtc
     1381 tgtcagcagg acgtgttcaa tcggttgctc tcaaattaat aatcgaccgt gaaaatgaaa
     1441 tcagcaattt caaaccagaa gaatattgga caattgatgc aaccttcaaa aaggggagca
     1501 agaaattcca ggcttctttc tacggtatag atggaaagaa aactaaaatt gaaaacaatg
     1561 agcaggttaa agaaattctc gcccgacttg atggggatga tttcttagta gaacaggttg
     1621 agcgtaagga acgcaaacga aatgcacctc tgccttacac aacttctact atgcagatgg
     1681 atgcggctaa aaaaatcaat ttccgtactc gtaaaaccat gatggttgcc caacaacttt
     1741 atgagggtgt cagtcttggc acaggtggta cccaaggttt gattacctat atgcgtacgg
     1801 attcaacacg tattagccct gttgcacaag cacaagctgc tgactttatc acggagcgtt
     1861 ttggtgctac ctattcaaag catggtaata aagtaaaaaa tgccagtggt gcacaagatg
     1921 cccacgaagc cattcgtcca tcaaatgtta acttaacacc tgaatcaatt gcaaaatatc
     1981 tggacaagga ccagttgcga ctatatacac tgatttggaa tcgttttgtc gcaagtcaga
     2041 tggctgcagc ggtctttgat accatgagtg ttcgtctagg tcaaaatgga gtgatttttg
     2101 ctgccaatgg tagccaagtt aaatttgatg gttacatggc agtctataat gattctgaca
     2161 agaataagat gttgccagat atggagcaag gtgatacagt tgtacgtaca tcaacaaatc
     2221 ctgaacaaca ttttacgcag ccaccagctc gctattctga agctactttg attaagactt
     2281 tggaagaaaa tggtgttggt cgtccgtcaa cctatgcacc aaccattgaa accattcaga
     2341 aacgctacta tgttaagtta gctgctaagc gatttgaacc gactgaactc ggcgaaatcg
     2401 ttaataagct gattgttgaa ttcttcccag atattgtcaa tgtcaaattt acagccgata
     2461 tggaacaaaa attggatgat gtagaaatcg ggaaagagca gtggcagaag gtcattgatg
     2521 gtttctatca gccattcaag gtagaattat ttaaggcaga agaacagatg gaaaaaatcc
     2581 aaattaagga tgaaccagct ggctttgact gtgatgtttg tggacatgaa atggtcatta
     2641 aactaggtcg ttttggaaaa ttctatgctt gtagtaattt ccccgattgc cgcaatacca
     2701 agcaaattac caaggagatc ggcgtaactt gccccgtgtg tcaacaaggc caggtcattg
     2761 aacgaaagag caaacgcaat cgacttttct atggttgtga ccgttatcca gagtgtgaat
     2821 ttacctcatg ggataaacct gttggtcgtg cctgtccaaa atgtgaccac tatcttgtcg
     2881 agaaaaaagt tcgtggtggt ggtaaacaag ttgtctgtcc aaatggtgac tacgaagaag
     2941 acaagattaa ataagattaa actgctattc tcaagggaat ggcagttgtt ttttatagtc
     3001 atgttagttt atcttagctt acttcgacaa gcgaggaaac ttcgtttcct tattttcaac
     3061 tttaaatgag gctggtcaaa aagtccaaag tcacttctca gtgcagtggt tgattgggtt
     3121 taacaggcca gtggagtgaa actgttcagt gaactgtttc agcctgagct tagaaatgaa
     3181 aaaatcgagg taaaaaggtt ggagataaga tttgcgaagc aaatcacagc aattcgtgtt
     3241 tcacattcca
//
LOCUS       DBNFCK010000148         3210 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.148, whole genome shotgun sequence.
ACCESSION   DBNFCK010000148 DBNFCK010000000
VERSION     DBNFCK010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.148"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            348..995
                     /locus_tag="AC1PIW_001790"
     CDS             348..995
                     /locus_tag="AC1PIW_001790"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIP6606686.1"
                     /translation="MNKMVIWFDLDGVIFDSYGVWDQVVQGILKHHGVAYTQQIREEL
                     WRIPMEDVNGYLLQLVENSLDENELERQKMNQLQSLYRDISLMDGVHELLSTLKAKGA
                     VMYAVTSNYLELAELGLSSHHLSNYFVKLFSSLNLFGSDKNREFYQFILHQERIDPAN
                     IVMVEDNKNNLCIGHELGIAGVYIEHPNSPFSDENTGIVSIKQLSELIPILEDRK"
     gene            992..1789
                     /gene="thiD"
                     /locus_tag="AC1PIW_001791"
     CDS             992..1789
                     /gene="thiD"
                     /locus_tag="AC1PIW_001791"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HIP6606687.1"
                     /translation="MTSLHVALSIAGTDPTGGAGIMADLKSFQARSVYGMAVVTSVVA
                     QNTKGVQLIRHLDQEILEEQLKSVFQDIVPDAIKTGMLAQVETIELVASYLAKQKCPY
                     VLDPVMVATSGDRLIEEDAIKALKTKLLPLATIITPNLPEAEVLVGRTLKSENDIMNA
                     GRWIRKELGVKNVVIKGGHLAKAASDYLFLEDGEISVISAARISTNHTHGTGCTYAAV
                     ITAELAKGRTVAKAVETAKRFITEAIQTALELGHGNGPVNHTTYRGE"
     gene            1794..2579
                     /gene="thiM"
                     /locus_tag="AC1PIW_001792"
     CDS             1794..2579
                     /gene="thiM"
                     /locus_tag="AC1PIW_001792"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HIP6606688.1"
                     /translation="MYLEKLRSQNPLTICITNDVVKNFTANGLLALGASPAMSEYPED
                     LEDLLPYTKGLLINTGTLTNETWELYKSALDIAEKYGVPTVLDPVAAGAGAYRKKVTL
                     DLLHHHTISLVRGNAGEIAALIGESIETKGVDSAQIDNVGELALRANQQLGIPVVITG
                     KKDAVAVNHQVRILENGSELMPLVTGTGCLLGAVLAAFIHLADEDSLLDCLEEVLSAY
                     SIAGEMAEQKTSLPGTFQIEFINSLYAIQSEEVEENKKVIHYE"
     gene            2572..>3210
                     /gene="thiE"
                     /locus_tag="AC1PIW_001793"
     CDS             2572..>3210
                     /gene="thiE"
                     /locus_tag="AC1PIW_001793"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HIP6606689.1"
                     /translation="MNRKMLQVYFICGTSDCPKGKFLDVLEKALQAGITCFQFREKGE
                     QGLTGADKLLLAKQVQHLCHRYQVPLIINDDVELARAIDADGIHLGQEDLSVVEARQL
                     FPGKIIGLSVGTKEEYLNSPIDLVDYIGSGPVFPTLSKDDASPAIGMDGLKQLRKLNS
                     DIPMVAIGGLSAKDCKEVLQAGADGIAVISAISHAEDPYKATKILVDGMQAMI"
BASE COUNT         1008 a          566 c          741 g          895 t
ORIGIN      
        1 tgtatcaaca aattttgttg acaagtacta tatttgtatt ctagtataga atactgtatt
       61 tactggtcat caatcatcgt aggtgtaact ccatttgata aaccatcaga tggagttttt
      121 tctattgtcg aatgaagagt tctcagaaat acggaattga gtgcaggtag ctcgaccagc
      181 ctagaggcta tactacgatg gaaataagat atgcgcaata agtcactttc gtagagttcg
      241 acaaggcgaa actgatgata aggctaatac aattaatagt attttcacaa atccccttag
      301 ccccttcaat ttacattttc accgagaaaa agagaaagga gatatagatg aacaaaatgg
      361 tcatttggtt tgaccttgat ggcgttatct ttgattccta tggggtctgg gatcaggtgg
      421 ttcaaggaat tttgaaacat cacggggttg cttataccca gcagataagg gaagaacttt
      481 ggcgtattcc aatggaagat gtcaacgggt atttattgca acttgtggag aattccttag
      541 atgagaatga acttgaaagg caaaaaatga atcaactgca atcactatat agagatattt
      601 ccttgatgga tggtgtgcat gaactcctat ctacattgaa ggcaaaaggt gcagtcatgt
      661 acgcagtcac ttcaaactat ctggagctgg ctgaactagg attgtcatcc catcatcttt
      721 caaactattt tgtgaaacta tttagcagtt tgaatctgtt tggaagcgat aaaaataggg
      781 agttttacca attcatccta catcaggaac ggatagatcc agccaatatc gtaatggtcg
      841 aggataataa gaacaatctt tgtattggac atgagttagg aatagcggga gtttatatag
      901 aacatccaaa ttcccctttt tctgatgaga atacaggtat agttagcatc aagcaattaa
      961 gtgaattaat accaattttg gaggatagaa aatgacatct ttacatgtag cattatcaat
     1021 agcaggaaca gatccaacag gcggagcagg cattatggct gatttgaaga gctttcaggc
     1081 tagatcagtt tatggtatgg cagtcgtgac gagtgtcgtt gctcaaaata ccaaaggggt
     1141 gcaactaatt cgtcatcttg atcaggaaat tttagaagaa caattgaaaa gtgtctttca
     1201 ggacattgtt ccagatgcaa taaaaactgg aatgttagca caagtcgaga ccattgagct
     1261 tgtcgcaagc tatcttgcaa aacagaagtg tccatatgtc ttggatcctg ttatggtcgc
     1321 aacgagtgga gaccgtttga ttgaagagga tgcaatcaaa gcgctgaaaa caaaattact
     1381 gccccttgct accatcatta caccgaatct accggaagca gaagttctag tcggtagaac
     1441 actgaaaagt gaaaatgaca tcatgaatgc gggccgttgg attcggaaag aacttggggt
     1501 aaaaaatgtg gtcattaagg gtggacatct ggcaaaggcg gcatctgact acttattttt
     1561 agaagatggt gagattagtg tcatcagtgc agcacgcatt tctacgaacc atacccatgg
     1621 tacaggttgt acctacgcgg cagtcattac tgcagagttg gcgaaaggtc ggacagtagc
     1681 aaaagcagtc gaaactgcca aacgatttat tacagaagca attcagactg ctctagaact
     1741 ggggcatgga aatggtcctg tcaatcatac cacctacaga ggagagtaac tagatgtatt
     1801 tggaaaaatt acgtagtcaa aatcccttga ccatttgtat tacgaatgat gtagtcaaaa
     1861 attttacagc taatggctta cttgctctcg gtgcctcacc tgcaatgagc gagtatccag
     1921 aagatttgga ggatctactt ccctatacaa aggggttatt gattaataca ggaaccttga
     1981 ccaatgaaac ttgggaacta tacaaatctg ccttggatat tgctgagaaa tatggtgtac
     2041 ctactgtctt agatcctgtt gcggcaggtg ctggtgccta tcgcaagaaa gttaccttgg
     2101 atctgttaca tcatcatact atttcactcg ttcgtgggaa tgcaggagaa attgcagcct
     2161 tgattggtga aagtattgaa acgaaagggg tagattctgc tcagattgac aatgtaggtg
     2221 aacttgctct cagagccaac caacaattag gaattcctgt tgtgattaca ggaaaaaaag
     2281 atgctgtcgc tgtgaatcat caggttcgga ttttggaaaa tggtagtgag ttaatgccac
     2341 tagtgacagg gacaggatgc ctgctgggag cagttctggc tgcctttatt cacctggctg
     2401 atgaggattc attattggat tgtttggaag aggtcctatc ggcttattcg atcgctggag
     2461 aaatggcaga gcagaaaacc tcactaccag gaaccttcca gattgagttt ataaatagcc
     2521 tctatgcaat ccaatcagaa gaggttgaag aaaataaaaa ggtcattcat tatgaataga
     2581 aaaatgcttc aagtttactt tatttgtggc acgtcagatt gtccaaaagg caaatttttg
     2641 gatgtactgg aaaaggcatt gcaggcagga atcacttgtt ttcaatttcg tgaaaaaggt
     2701 gagcaaggtt taacaggggc tgataaacta ctgttggcaa aacaagttca acacctgtgt
     2761 caccgctatc aggttccatt aatcattaat gatgatgttg agttagcacg tgccattgat
     2821 gctgatggaa tacaccttgg acaggaagat ttgtcagtag tagaagcgag acagctcttc
     2881 ccagggaaaa taattggact atcagttgga acaaaagaag aatacctcaa ttcgcctatt
     2941 gatctagttg attatatcgg aagcggacct gtttttccta ctctatctaa ggatgatgct
     3001 agcccagcca ttggaatgga tggattaaag caattaagga aactcaattc cgatattccc
     3061 atggttgcga taggagggtt atctgctaaa gattgtaagg aagttctcca agcaggtgca
     3121 gatggcatag ctgttatctc agccatttcc catgctgaag acccctacaa agcaaccaag
     3181 atattggtgg atgggatgca agcaatgatt
//
LOCUS       DBNFCK010000149         3171 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.149, whole genome shotgun sequence.
ACCESSION   DBNFCK010000149 DBNFCK010000000
VERSION     DBNFCK010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.149"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..472
                     /locus_tag="AC1PIW_001794"
     CDS             <1..472
                     /locus_tag="AC1PIW_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIP6606690.1"
                     /translation="NDVNYPALMVFNGLLGAFSHSKLFMNVREKESLAYTIGSQVSIF
                     SGMLKVYAGISRENRLRVMKLISKQLLDLKCGKFTEEELELTKNMLIHSATLAQDRQN
                     NLIEQVYNQVTLGNRNLSWLDWIEAIKSVSIDDVIQVGQMINLQAVYFMEGTEE"
     gene            469..1752
                     /gene="yfmH"
                     /locus_tag="AC1PIW_001795"
     CDS             469..1752
                     /gene="yfmH"
                     /locus_tag="AC1PIW_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P 5-aminopentanol modification-associated
                     protein YfmH"
                     /protein_id="HIP6606691.1"
                     /translation="MKLVEKKYPYMKESVFYGKVDNGLTVILLPKVDFHETYGILTTN
                     FGGIHTRFTLANGNSVVYPAGIAHFLEHKLFETENEEDIMNEFAKLGASANAYTSFRQ
                     TSYLFSTTQKVLESLSLLQSFVREPYFTEDNVEREQGIIEQEIEMYQDDADYRLFTGI
                     LSSLYPESPLAYDIAGTVESIAAITADDLHENFDVFYHPSNMNLFVIGNFDLEAVWKQ
                     ISSYQVAQMDNPAQSFELAGIQKLPIQEHLSEQFEVSTPKLAVGLRGNDEVDKETIQK
                     YRLSLQFLFAMLFGWTSKRYQQLYEQGKIDSSFQFQLEVTPEYHYLIISGDTQEPITL
                     SSILMKALRKFEDDADVTEDHLQLLKNEMYGDFIRSLNSLEFTASHFVSQYSEYENVF
                     DIPQMIQSLQLDDIREAGRRFIDHCDMTDFTIFPK"
     gene            1834..2691
                     /gene="rodZ"
                     /locus_tag="AC1PIW_001796"
     CDS             1834..2691
                     /gene="rodZ"
                     /locus_tag="AC1PIW_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HIP6606692.1"
                     /translation="MRQKSIGEVLRTAREGRGWTFVDLQRITKIQAKYLQALEYNDFD
                     FIANSDVVQSILKAYAEALELDADVLLDAYETNSLVKYYEDGEEELRSSELKRSYKVR
                     KRKKNSYLPLIYLLLATGLIIIFVIYIVHSRLKNRASITPTTTSYSIVEQSVSEASSG
                     TETSSTEQAQSNSTSSSSSATENVTISGSGSNIVATLKTVTYPVEVTVTAKNTTSWIS
                     LSDTLLEGGVTLTPDNPTVTTTIEEGVTSTTLILGVVKGVEVSVGGQKLELSALTADT
                     GSITINFEQ"
     gene            2701..>3171
                     /gene="pgsA"
                     /locus_tag="AC1PIW_001797"
     CDS             2701..>3171
                     /gene="pgsA"
                     /locus_tag="AC1PIW_001797"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HIP6606693.1"
                     /translation="MKKENIPNALTIGRILVIPIFILLLTVWNSTISHVLAALIFALA
                     SITDYLDGYLARKWQVVTNFGKFADPMADKILVMTAFIMLVELGFAPAWVVAIIICRE
                     LAVTGLRLLLVENGGTVLAAAMPGKIKTFSQMFAVIFLLLHWNVVGQITLYIALF"
BASE COUNT         1003 a          480 c          685 g         1003 t
ORIGIN      
        1 caacgatgta aactatccgg ctttgatggt atttaatggt ctactgggtg ctttctccca
       61 ttcgaagtta tttatgaatg ttcgtgagaa agaaagtttg gcctatacaa ttggtagtca
      121 ggtctctatt ttttcaggaa tgctaaaggt ctatgctgga attagccgtg aaaatagact
      181 cagggtaatg aagttaatta gtaaacaact acttgattta aaatgtggta agtttacaga
      241 agaagaatta gagttgacaa aaaacatgtt gattcattca gcaaccttgg ctcaagatag
      301 gcagaataat ttgatagaac aagtatataa tcaagttacc ttaggaaata gaaatttaag
      361 ttggttagat tggattgagg ctatcaaatc ggtatcaata gacgatgtca ttcaagtagg
      421 acagatgatt aatttacagg ctgtttactt tatggagggg acagaagaat gaagttagtt
      481 gagaagaagt atccttacat gaaagaatct gtcttttatg ggaaagttga caatgggttg
      541 acagttattt tgcttcctaa agtagatttc catgagacat atggaattct aacgacaaat
      601 tttgggggta ttcatactcg atttacattg gctaatggta atagtgttgt ttatcctgca
      661 gggattgcac attttttaga acataagctg tttgaaactg agaatgaaga ggatattatg
      721 aatgagtttg ctaaattggg agcaagcgca aatgcttata ccagttttag acagactagt
      781 tacttatttt caacgactca gaaagtattg gagtctctat cacttttaca atcttttgtt
      841 cgagaaccgt attttacaga agacaatgta gaaagagaac aggggattat tgaacaagaa
      901 atcgagatgt atcaagatga tgcagattat cgcttgttta cgggaattct atcatcgctt
      961 tatccagaaa gtcctttggc ctatgatata gcagggacag ttgaatcaat tgctgcaatt
     1021 actgcggatg atttacatga gaattttgat gtgttttatc atccaagtaa catgaattta
     1081 ttcgttattg gtaattttga tttagaggca gtttggaaac aaatttcaag ttatcaagta
     1141 gctcagatgg ataatccagc tcagtcgttt gaattggcag gtatccaaaa gttgccgata
     1201 caggaacatc tctcagaaca atttgaagta tctaccccaa aattggctgt tggtttacgg
     1261 ggaaatgacg aggttgataa ggagactatt cagaaatatc ggcttagttt acaattttta
     1321 tttgccatgt tgtttggttg gacttcaaaa cgctatcaac agttatacga gcagggaaaa
     1381 attgattctt ctttccagtt ccaattagag gttacacctg aataccacta tttgattatc
     1441 tcgggagata ctcaagaacc cattacgctt tctagtatac tgatgaaagc acttaggaaa
     1501 tttgaggatg atgcagatgt gacagaggat catttacaat tgttgaaaaa cgagatgtat
     1561 ggagatttta ttcgtagctt aaattctctt gagtttacag ctagtcactt tgtttctcag
     1621 tattcagaat atgaaaatgt ttttgatata cctcaaatga tacaaagtct tcagttagat
     1681 gacatcagag aggcaggtcg tcgctttatt gatcactgtg atatgacaga tttcacgatt
     1741 tttccaaaat aagtgggcgc aacccgtcga aatttccttg gaattttgtt agaatagtat
     1801 actgtacatg atgagcgata gagagagggt attatgagac aaaagagtat aggggaagta
     1861 cttagaacag ccagagaagg acggggttgg acatttgttg acttgcaacg gataacaaaa
     1921 atccaagcta aatatttgca ggcacttgag tacaatgatt ttgattttat tgcaaactca
     1981 gatgttgttc agtctatttt aaaagcatac gctgaggctt tggaattaga tgcagatgtt
     2041 ctactggatg cttacgagac caatagttta gtgaaatatt atgaagatgg agaagaagaa
     2101 ctacgctctt cagaattgaa aagaagctat aaggttcgta aacgtaaaaa gaactcttat
     2161 cttccgttga tttacttatt acttgcgacg gggttgatta ttatttttgt gatttatatt
     2221 gttcatagca ggctcaaaaa tagagcatct attacgccta ctacaacgtc atatagtata
     2281 gttgagcaat ctgtatctga agcaagtagt ggcacagaga cgagttctac tgagcaggcg
     2341 cagtcaaatt caacgagttc aagtagttcc gctacagaaa atgtgactat ttcaggatca
     2401 ggttctaaca ttgttgcgac ccttaaaaca gtgacttatc cagtagaagt aactgtaaca
     2461 gcaaaaaata caacgagttg gatcagcctt tctgacacct tgttagaggg gggcgttaca
     2521 ctaacacctg ataatccaac agtaactact actattgaag aaggagtgac ttctactaca
     2581 cttattcttg gtgttgttaa aggggttgag gtaagtgtag gtggtcaaaa gctagaacta
     2641 tcagctctga cagcagatac aggctcaatt acaataaatt ttgaacaata aaggttttat
     2701 atgaaaaaag aaaatattcc caatgcattg acgataggta ggattttggt aattccaatt
     2761 tttatcttgt tactgaccgt ttggaattca acaattagtc atgtcttagc agcacttatt
     2821 tttgcattag ctagtattac ggattatcta gacggctact tggctcgtaa atggcaggtt
     2881 gtaacaaact ttggaaaatt tgcggatccg atggcagata agattttggt gatgacagca
     2941 tttatcatgc ttgtagagct tggctttgct ccagcttggg tagttgcaat tattatttgt
     3001 cgtgaattgg cagtgacagg tcttcgtcta ctcttggtgg aaaatggagg aacagttcta
     3061 gctgcggcta tgccaggaaa aatcaaaaca ttttcacaga tgtttgcagt tattttcctg
     3121 ctcttacact ggaacgtcgt ggggcaaata acactctata ttgcactttt c
//
LOCUS       DBNFCK010000150         3170 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.150, whole genome shotgun sequence.
ACCESSION   DBNFCK010000150 DBNFCK010000000
VERSION     DBNFCK010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.150"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..184
                     /locus_tag="AC1PIW_001798"
     CDS             <1..184
                     /locus_tag="AC1PIW_001798"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606694.1"
                     /translation="AQEDGYGMKNIRLKLARVEKELEKWSNNKQFSSCSAIFLKNKNL
                     QNSLPKVRAYVTISKA"
     gene            299..1576
                     /locus_tag="AC1PIW_001799"
     CDS             299..1576
                     /locus_tag="AC1PIW_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HIP6606695.1"
                     /translation="MSTKANLLFDVHEKPAPLQGILLSFQHVFAMFGATILVPLILGM
                     PVSVALFASGVGTLIYQVATQFKVPVYLGSSFAYISAMALAIKEMGGDVSAAQTGILF
                     VGLIYVLIAALVKVIGTKWIDTLLPPIVIGPMIIVIGLGLANSAVTSAGFVADGDWKN
                     VVVAIATFLIAAFVNTKGKGFAKIVPFLIAIIGGYVIALCLGLVDFTPVLEAAWFELP
                     GFYLPFETGAFKSYNFYFGPEMIAILPIAVVTVAEHIGDHTVLSQICGRQFLKDPGLS
                     RTLIGDGVATAVSAFIGGPANTTYGENTGVIGMTRIASVSVIRNAALIAIAFSFLGKF
                     TSLISTIPSAVLGGMSILLYGVIASNGLKVLIESRVDFGQVRNLIIASSMLVLGLGGA
                     VLNIGAITLSGTALSAIVGIILNLILPKAEKAE"
     gene            1780..2049
                     /gene="rpsO"
                     /locus_tag="AC1PIW_001800"
     CDS             1780..2049
                     /gene="rpsO"
                     /locus_tag="AC1PIW_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIP6606696.1"
                     /translation="MAISKEKKNEIMAQYARHEGDTGSVEVQVAVLTWEINHLNDHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRRTDVNRYRELIHSLGLRR"
     gene            complement(2200..3027)
                     /locus_tag="AC1PIW_001801"
     CDS             complement(2200..3027)
                     /locus_tag="AC1PIW_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIP6606697.1"
                     /translation="MNRNILNHSIENLTPTENKLLDYIEENIHRIETMNIYQLADNAG
                     VSTATISRISKKMGYSNFNKLKIEIAKSKYDDLLNAHFTPSDSIDDISTKLIHSITIS
                     NQQTVSFLNLEELEAAVKLLQSASRIYLFGVGASGIVCSDFYYKLSRIGKTCIFAQDT
                     HIQMANIATAGNGDLAIGISYSGMTKEVVEPLRFAKENGVSTITITGTGKNQLADLAD
                     ITFRIPRHEHELRVGAITSRSNSLFLTDLLYLALIQDHKQEITAILEQTKHLTEVIK"
BASE COUNT          880 a          623 c          663 g         1004 t
ORIGIN      
        1 tgcacaggag gatggctatg gcatgaaaaa tattcgttta aagttagctc gtgtggaaaa
       61 agagttggaa aaatggagta ataataagca gttcagctcc tgctcagcca tttttctaaa
      121 aaataaaaat ttacaaaatt ccttgccaaa agtaagggct tatgttacaa tatcaaaagc
      181 gtaggttttt aaactcatcc cgtgaggtga gaaagacaac aggataattc attaagggcc
      241 tatgactatg tatatttaga aagtctgggt ctttttgtgt acccagaatg ggagatttat
      301 gagtacaaaa gccaatcttt tgtttgatgt ccatgagaaa ccagcaccgc ttcaagggat
      361 tttgttgagt ttccagcacg tgttcgctat gttcggagcg acaatcttgg tgccactgat
      421 tctcggtatg ccagtttccg tggctctctt cgcatcaggt gtcggaacct tgatttacca
      481 agtagcgaca cagtttaagg tgccggttta cctgggatct tccttcgcct acatctcagc
      541 tatggccctc gccattaagg aaatgggcgg cgatgtgtca gctgcgcaga caggtattct
      601 ctttgtcggt ttgatttacg tcttgattgc tgcgcttgtc aaagttatcg ggaccaagtg
      661 gattgatacc ctcttgccac cgattgttat cggtcctatg attatcgtta tcggtctggg
      721 tcttgccaac tctgccgtga cttcggcagg ctttgtcgca gacggtgact ggaaaaacgt
      781 cgttgtggcg atcgcaacct tcttgattgc tgcttttgtc aataccaaag ggaaaggctt
      841 cgccaagatt gttcccttct tgattgccat catcggtggt tacgtgattg cactctgtct
      901 tggtttggtt gacttcacac ctgttctgga agcagcttgg tttgaattac caggtttcta
      961 cctaccattt gaaacaggtg ccttcaagtc gtacaatttc tacttcggtc cagaaatgat
     1021 tgctatctta ccaattgctg ttgtaacagt agctgagcat atcggtgacc acaccgttct
     1081 cagccaaatc tgcggccgcc agttcttgaa agatccaggc ctcagccgta ccttgattgg
     1141 tgacggtgtg gcgactgccg tatctgcctt tatcggtgga cctgctaaca cgacttatgg
     1201 tgaaaataca ggggttatcg gtatgactcg catcgcatca gtatccgtta tccgtaatgc
     1261 agccttgatt gccattgcct tctcattctt gggtaaattt acatccctta tctctactat
     1321 tccaagtgcc gtacttggag gcatgtctat cttgctttac ggggttatcg cctcaaacgg
     1381 tttgaaagtt ttgattgaga gccgtgttga tttcggtcaa gtccgtaacc tgattattgc
     1441 aagctcaatg ctggtattgg gtcttggtgg agcagttctc aacatcggtg ccatcaccct
     1501 ttcaggtaca gccctctcag ccattgtagg tattatcctc aatctcatct tgccaaaagc
     1561 agaaaaggca gagtaaatgt gatatacaag cagaaagtca gcaaaagctg gctttttttg
     1621 aaaatagcag agctactcag ctagttttca aatcagttga caaaaaagac aatccttgat
     1681 agaataaatg aggtccaaaa ggacaaatct agctttttct tggttacagg tcgccaacct
     1741 gtcactcgga ttaggctcaa aaacaaagga gaacatatca tggcaatctc aaaagagaaa
     1801 aaaaatgaaa tcatggcaca atatgctcgt catgaaggcg acacaggttc agttgaagtt
     1861 caagtagcag tacttacttg ggaaatcaac caccttaacg accacatcaa acaacacaaa
     1921 aaagaccacg caacttaccg tggtttgatg aaaaaaatcg gtcgccgtcg taacttgttg
     1981 gcatacctac gccgcacaga cgttaaccgt taccgtgaat tgattcactc actcggactc
     2041 cgtcgttaat tagcaggtat agaaacaaaa aaacacagca ccagtatctt gtatactgag
     2101 ctgtgttttt tgtttttttc cctattgtat gagttgcttc aataaaagat tcgatcgagc
     2161 ttaggggatt tgctttttgt agtggattta tcaggtggtc tatttgatta cttctgtaag
     2221 atgttttgtt tgttctaaga tagctgtaat ctcttgttta tgatcctgga taagtgctag
     2281 gtaaagcaag tcggttagga aaagtgagtt gcttcgtgat gtaatagccc cgactcgaag
     2341 ttcatgctca tgtcgtggga ttctaaatgt aatatcggct aaatctgcta attgattctt
     2401 accagttcca gtgatagtta tcgttgaaac tccattttct tttgcgaaac gaagtggctc
     2461 aacgacttct tttgtcattc cgctataact aattccgatt gctaagtcac cgtttccagc
     2521 ggttgcaata ttagccatct gaatatgtgt atcttgtgca aaaatacatg ttttacctat
     2581 tctactcagc ttgtagtaaa agtcactgca aacaatacct gatgccccaa ctccaaataa
     2641 ataaattcga gaagcgcttt gtaataattt tacggcggct tctaattctt ccaagtttag
     2701 aaaactgacg gtttgttgat ttgaaattgt tattgaatga attaatttag tagaaatatc
     2761 gtcaatcgaa tctgatggcg tgaagtgagc attgagtaaa tcatcatatt tcgatttggc
     2821 tatttctatc tttaacttat taaagttaga atagcccatt tttttactga ttcgactgat
     2881 tgtagctgtt gatactccgg cgttatcggc taattgatag atattcattg tttcaatacg
     2941 atggatgttc tcttcaatat aatccaataa tttgttttcg gtaggggtta aattctctat
     3001 cgagtgattt aaaatgtttc tattcataat gattcccttc taacacagtc tgttataaag
     3061 tttctttaat ttctaaaaaa tattgaaata ataatacaaa agttgtataa tacgattata
     3121 gcaaacgttt acaaaaaaag aaaggagaat aagatggata ttgaacgatt
//
LOCUS       DBNFCK010000151         3123 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.151, whole genome shotgun sequence.
ACCESSION   DBNFCK010000151 DBNFCK010000000
VERSION     DBNFCK010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.151"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..275)
                     /locus_tag="AC1PIW_001802"
     CDS             complement(<1..275)
                     /locus_tag="AC1PIW_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HIP6606698.1"
                     /translation="MSKKEILLDFIEKNNGIVTNKDCKALGIPTIYLTRLEKEGIIFR
                     VEKGIFLTQNGDYDEYYFFQYRFPKAIFSYISALYLQQFTDEIPQYF"
     gene            445..1611
                     /locus_tag="AC1PIW_001803"
                     /pseudo
     CDS             445..1611
                     /locus_tag="AC1PIW_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167702.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative cross-wall-targeting lipoprotein signal
                     domain-containing proteiin"
     gene            1678..1761
                     /locus_tag="AC1PIW_001804"
     CDS             1678..1761
                     /locus_tag="AC1PIW_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HIP6606699.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            1886..2623
                     /gene="erm(B)"
                     /locus_tag="AC1PIW_001805"
     CDS             1886..2623
                     /gene="erm(B)"
                     /locus_tag="AC1PIW_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HIP6606700.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
     gene            2793..3053
                     /locus_tag="AC1PIW_001806"
                     /pseudo
     CDS             2793..3053
                     /locus_tag="AC1PIW_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
BASE COUNT         1143 a          544 c          583 g          853 t
ORIGIN      
        1 tcaaaatatt gtggaatttc atctgtaaac tgttgtagat acagtgccga aatataagag
       61 aaaatagcct taggaaaacg atattgaaag aagtagtatt catcatagtc tccgttttgg
      121 gttaggaaaa tacctttttc aactcggaag ataatgcctt ctttctctag tcttgtcaga
      181 tatattgttg gaatacctag tgctttacaa tctttgtttg tgacaattcc attgttcttt
      241 tctataaaat cgagtagaat ctcttttttt gacatacggc ctcccacttg ttgcttttac
      301 actacaattt tacaatattt gtggtataaa agcaacattt tcttttccct cattttattg
      361 tgataacaaa atcaagcctt cttttttatg atgaggaaag agtgacaatt tgttgctaca
      421 taaaaaagaa aagaaggact atttatgacc aaaacatgta atcatcactt tcttgtcaat
      481 caggaaaaag gcgagaaaca cgtctttcgc aagagtaaaa aatatcgcac tctatgttcc
      541 gttgcccttg gaaccatggt gacggctgtt gttgcttggg gaggaacggt tgcacatgct
      601 gatgaagtca catcatcagt tgacaccaac atccaacgaa cggagaatcc agccacaaat
      661 ttaccagaag cacaaccaac ccctgtttct gaacaaactg aaagtatggc atcaactgga
      721 caatctaacg gtgcaattgc tgtcaccgta ccacatgata cggtaaaaca agcagtggaa
      781 caggcaaagg ctgaaggtgt ttctacggtt gaagatagcc caatggattt ggggaataca
      841 acttctgcgt cagagaccag ccaacaaatt tcaaaagcag aagcagatgc ccaaaaccaa
      901 gttgtggcta tcaatgaagt tactgaaagc tacaaagctg acaaagcgac atacgaatcg
      961 aataaagccc gcattgaaca ggaaaatatg gagctgtcac aggtctacga aggggcaaac
     1021 caaactggta aagagacaaa tgcttgggtt gatactaaag tcaatgacct aaaatcccag
     1081 tatgcagatg ctgatgtgac agtaattgaa caagtagttt catcaggaaa tgggacatct
     1141 gtacttgact atacaaacta tggcaaggct gttgaaacca ttcaatcaac taacgaacaa
     1201 gctgtagcag attatctaac aaagaaaaca aaggcagatg atattgttgc gaaaaatcag
     1261 gccattcaac aagaaaatga agctggactt gctaaggcaa aggcagataa tgaagccatt
     1321 gaaaggcgta atcaggctgg tcaagcagct gttgatgctg aaaaccgtgc aggtcaagcc
     1381 gcagtagatc aagcgaatca ggagaaacaa caattagttt cagatcgtgc agctgagatt
     1441 gaagctatta caaaacgtaa taaagaaaaa gaagccgcag ccagaaaaga gaatgaagcg
     1501 attgatgcct acaatgccaa agaaatggaa cgctaccaac gtgatttggc cgagatttca
     1561 aaaggtgaag aaggttatat ttctgaagcc cttgctcaag ccctcaaaat tttgtataat
     1621 aggaattgaa gttaaattag atgctaaaaa tttgtaatta agaaggaggg attcgtcatg
     1681 ttggtattcc aaatgcgtaa tgtagataaa acatctactg ttttgaaaca gactaaaaac
     1741 agtgattacg cagataaata aatacgttag attaattcct accagtgact aatcttatga
     1801 ctttttaaac agataactaa aattacaaac aaatcgttta acttctgtat ttgtttatag
     1861 atgtaatcac ttcaggagag attacatgaa caaaaatata aaatattctc aaaacttttt
     1921 aacgagtgaa aaagtactca accaaataat aaaacaattg aatttaaaag aaaccgatac
     1981 cgtttacgaa attggaacag gtaaagggca tttaacgacg aaactggcta aaataagtaa
     2041 acaggtaacg tctattgaat tagacagtca tctattcaac ttatcgtcag aaaaattaaa
     2101 actgaatact cgtgtcactt taattcacca agatattcta cagtttcaat tccctaacaa
     2161 acagaggtat aaaattgttg ggaatattcc ttaccattta agcacacaaa ttattaaaaa
     2221 agtggttttt gaaagccgtg cgtctgacat ctatctgatt gttgaagaag gattctacaa
     2281 gcgtaccttg gatattcacc gaacactagg gttgctcttg cacactcaag tctcgattca
     2341 gcaattgctt aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa acagtgtctt
     2401 aataaaactt acccgccata ccacagatgt tccagataaa tattggaagc tatatacgta
     2461 ctttgtttca aaatgggtca atcgagaata tcgtcaactg tttactaaaa atcagtttca
     2521 tcaagcaatg aaacacgcca aagtaaacaa tttaagtacc attacttatg agcaagtatt
     2581 gtctattttt aatagttatc tattatttaa cgggaggaaa taattctatg agtcgctttt
     2641 ttaaatttgg aaagttacac gttactaaag ggaatggaga taaattatta gatatactac
     2701 tgacagcttc caagaagcta aagaggtccc tagcgcctac ggggaatttg tatcgataag
     2761 gggtacaaat tcccactaag cgctcgggac cccttgtagg aaaatgtcct aagtgtggca
     2821 acaatattgt attaaaaaaa tcgttttatg gttgttcaaa ttatcctgaa tgtaagttta
     2881 ctttagctga acattttaga aagaaaaaac taaccaaaac gaatgtaaaa gaattactgg
     2941 agggaaaaga aaccctggta aaaggaatca aaaacaaaga gaaaaagccc tacaatgccg
     3001 ttgtaaaaat tggggaaaag ggatatattg attttatctc tttttcaaaa taaacataaa
     3061 agccctttaa agagggcttt tatatattaa tcacaaatca cttatcacaa atcacaagtg
     3121 att
//
LOCUS       DBNFCK010000152         3119 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.152, whole genome shotgun sequence.
ACCESSION   DBNFCK010000152 DBNFCK010000000
VERSION     DBNFCK010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.152"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            23..856
                     /locus_tag="AC1PIW_001807"
     CDS             23..856
                     /locus_tag="AC1PIW_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIP6606701.1"
                     /translation="MSKIKIVTDSSTTIEPSLVEELNITVVPLSVMVDGVVYSDNDLK
                     EGEFLELMRGSKNLPKTSQPPVGLFAETFAELAKDGSQIIAILLSHALSGTVEAARQG
                     ATLSGADVTILDSSFTDQAEKFQVVEAARMAQAGASKEEILAAIETVKEKTELYIGVS
                     TLENLVKGGRIGRVQGMLSSLLNIRVIMEMKDHQLTPIVKGRGNKTFKKWLEEFTATL
                     ADKKVAEIGISYSGTADFANEMKSQLQEYVSKEISVLETGSIIQTHTGEDAWAILLRY
                     E"
     gene            849..1682
                     /locus_tag="AC1PIW_001808"
     CDS             849..1682
                     /locus_tag="AC1PIW_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIP6606702.1"
                     /translation="MNNRKLIQGFVFFFLTILGSIFLFHQLIPVASSRISRQELTVSE
                     KISFRYVALGDSLTEGVGDTTGQGGFVPLLAQSLTNDYGYEVDYKNFGVSGNTSNQIL
                     KRMKEDGELISYLKTADLLTLTVGGNDLRKAIIKNIANLKVSTFDKPAKDYGKQLDTI
                     IKTARKNNPNLPIYVVGIYNPLYLNFPELTEMQTIVDNWNTMTEQITEKYQHVYFVPI
                     NDLLYKGLEGEAGISQNGSQVTNNLLYEEDSFHPNNTGYEIMKKAILERMNETTESWE
                     P"
     gene            1654..2250
                     /locus_tag="AC1PIW_001809"
     CDS             1654..2250
                     /locus_tag="AC1PIW_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HIP6606703.1"
                     /translation="MRPRKAGNLNIWKWLFLAQLSLLIGCGIVLYTRIQTDREDLTKL
                     VQTSTEDTKVGTFSTNREQLNQTLTNYLKEYQTEEFSYQLFVTSQQVVFEGSYQIFGV
                     TIPLYIYFQPSKMEDGSILLRITEISVGSLSLPKAEVLAYLQKNYKLPAFVKIDSEQA
                     QVQVQLTELKNKFGLYGKANTIDLYNDQFIVDIYRKRQ"
     gene            2340..2615
                     /locus_tag="AC1PIW_001810"
     CDS             2340..2615
                     /locus_tag="AC1PIW_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIP6606704.1"
                     /translation="MANKQDLIAKVAEATSLTKKDSAAAVDAVFGAVADYLAAGEKVQ
                     LIGFGNFEVRERSARTGRNPQTGAEIKIAASKVPAFKAGKALKDAVK"
     gene            complement(2902..>3119)
                     /locus_tag="AC1PIW_001811"
     CDS             complement(2902..>3119)
                     /locus_tag="AC1PIW_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HIP6606705.1"
                     /translation="RKILFKCIHNIASASHTNPCHIADFYEKRKRQSQTTSTKPHTIA
                     SIHRLIRTMYYLIMHNKLYDYASTQNR"
BASE COUNT         1033 a          536 c          666 g          884 t
ORIGIN      
        1 gaagagaatt gtaggatgaa atatgtcaaa gattaagatt gttacggatt caagtacgac
       61 tatcgaaccc agtttggtcg aagaattgaa tataacagtt gttccattat ctgtaatggt
      121 tgacggagtc gtatactctg acaacgattt aaaagaagga gaattcttag agctcatgcg
      181 tgggtcgaaa aacttgccaa aaacaagcca gcccccagta ggactctttg cggaaacttt
      241 tgctgaattg gctaaagacg gcagtcagat tattgccatt ctcttgtcgc atgccttgtc
      301 agggacagta gaagctgcta gacaaggagc aaccctttct ggtgctgatg tgacaatact
      361 cgattcaagc tttacagatc aggctgaaaa attccaagtt gtagaagcag cacgcatggc
      421 acaagctggt gccagcaaag aagaaatcct tgcagctatt gaaacagtca aagaaaaaac
      481 ggaattatac atcggtgtat caaccctaga aaatctagtc aagggtggtc gtattggacg
      541 tgttcaaggg atgctgagta gcctactcaa tatccgtgtc atcatggaaa tgaaagacca
      601 tcagctgact ccgattgtca agggacgagg caataagacc tttaaaaaat ggctggaaga
      661 gttcacggca actctagcag ataaaaaagt agccgagatt ggtatttcct attcaggaac
      721 ggcagatttt gcaaatgaaa tgaagagtca attacaagag tacgtctcaa aagaaatttc
      781 tgttttagag acaggttcca tcattcaaac tcatactggt gaggatgcct gggctatttt
      841 gcttcgttat gaataatagg aagttgattc aaggctttgt tttctttttt ctgacaattc
      901 tagggtcgat ttttctattt catcagctca ttcctgttgc ctcatctcgc atctccagac
      961 aagagcttac cgtatcagaa aaaatcagtt ttcgatatgt ggcacttggt gattctttga
     1021 cagaaggtgt tggagataca actggacagg ggggatttgt tcccttgttg gctcaatctc
     1081 taactaatga ttatggttat gaagtagatt ataaaaattt cggagtttct gggaatacca
     1141 gtaatcagat tcttaaacga atgaaggaag atggagagct gataagttac ttaaaaacag
     1201 cagatctgtt aactctgaca gttggtggca acgatttgcg caaggctatt attaaaaaca
     1261 ttgccaatct caaagtttca acttttgata agcccgcaaa agattacgga aaacaattgg
     1321 atacaattat taagacagcg cgtaagaata atccaaatct tcccatctat gttgtcggga
     1381 tttacaatcc actctacctc aattttcctg aattgacaga aatgcagaca attgttgata
     1441 attggaatac gatgacagaa cagataactg aaaaatatca gcatgtctac tttgttccga
     1501 tcaatgattt gctttataaa ggattggaag gggaggctgg tattagtcaa aatggtagcc
     1561 aagttaccaa taatttactg tatgaggaag acagtttcca tcccaataat actggatatg
     1621 agattatgaa aaaagcgata ttggagagaa tgaatgagac cacggaaagc tgggaacctt
     1681 aatatttgga aatggttatt tttagcccag ttgtctttat tgattggttg cggaatagtc
     1741 ctctatacac ggattcaaac agatagagag gatttaacaa agctcgttca gactagtaca
     1801 gaagacacaa aggttgggac cttctccact aatagggaac agctcaacca aaccctcact
     1861 aactatctaa aagagtatca gacggaagag ttttcctatc aattattcgt aacgagtcag
     1921 caagttgttt ttgaaggcag ctatcagatt tttggtgtga ctatcccact ctatatttat
     1981 tttcaaccta gcaaaatgga agatggcagt attttgttac ggataacaga aatatcagtt
     2041 ggaagtttat ccttgccgaa agcagaggta ttagcttatt tacaaaaaaa ttataaacta
     2101 cctgcctttg taaaaataga ctcagaacaa gcgcaagtac aggttcaact gacagaactg
     2161 aagaacaaat ttggtctgta tggaaaggcg aatacgattg atttgtacaa cgaccaattc
     2221 attgttgata tctacagaaa aagacagtaa aatagcatta aaaacttgac caaacgccta
     2281 aaattcggta ctatatagaa gtgagtaata actcagaaaa ttattggagg acttagcaaa
     2341 tggctaataa acaagatttg attgcaaaag ttgcagaagc aacttcattg actaaaaaag
     2401 actcagcagc agctgttgat gcagtattcg gagcagtagc agactacctt gcagctggtg
     2461 aaaaagtaca attgatcggt ttcggtaact ttgaagttcg tgagcgttca gcacgtacag
     2521 gccgcaaccc tcaaacaggt gcagaaatca aaatcgcagc atctaaagta cctgctttca
     2581 aagctggtaa agcacttaaa gacgctgtaa aataaaacag tctggggata gactgtttta
     2641 tccctcgctt aaaaacaaaa gagcgaggct aagcatcaaa aagcccgtca tatcaagctt
     2701 tctagcttgt ccgacgggct ttttttctac tatttgtcaa gtccatcaag gtatttgacg
     2761 aaaaatattt tgagttcaat agtcaaaata aggaaattgt tttattttgg actacatttt
     2821 agtgtaaaaa gtgcatacaa aaccaacacc ttatgttgaa attttttgat aaggtgttac
     2881 aatgatagag cataaacagt tttaccgatt ttgggttgaa gcgtaatcgt aaagtttgtt
     2941 atgcataatg aggtaataca ttgtccgaat gagacgatgt atggaggcaa tcgtgtgcgg
     3001 cttcgttgaa gtcgtttgcg attgtctttt tcgtttctca taaaagtctg cgatatggca
     3061 aggattggta tgactggctg aagcgatatt gtggatacac ttgaacagaa tctttctag
//
LOCUS       DBNFCK010000153         3103 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.153, whole genome shotgun sequence.
ACCESSION   DBNFCK010000153 DBNFCK010000000
VERSION     DBNFCK010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.153"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            8..2206
                     /locus_tag="AC1PIW_001812"
     CDS             8..2206
                     /locus_tag="AC1PIW_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HIP6606706.1"
                     /translation="MKKKLLMLLASILPVFFIFTGIKADDTIDVVFDNAYAPFEFKDS
                     DQIYKGLDVDIINEVAKRSGWTMNQSFPGFDAAVNAVQAGSADALMAGTTITEARKKV
                     FTFSDPYFDTKIVIATTKANTISSYKDLKGKTVGVKNGTAAQNFLEENKDKYGYNVKT
                     FDTGDLMYNSLSAGAVDAVMDDEAVIQYAIQQGQDLSIDIEGEAIGSFGFSVKKGSQY
                     EYLVEDFNKALAEMKKDGTYEIIMNKWLGASTTSTESTDYSSRLSLTGNASAKATPVK
                     SSYTIVADSSFAPFEYQDESGNYVGIDMELIKAIAEHQGFTITIQNPGFDAALNAVQA
                     GQADAVIAGMSITDARKEIFDFSNAYYTSNILLAVKNGSDIASYEDLKGKTVGAKNGT
                     ASYSFLEENKSKYGYTLKAFDEASSMYDSLNSGSIDALMDDEAVLLYAIQQGRNFATP
                     IPGEKSGEYGFAVKKGANPELIEMFNNGLAALVESGKYDEILNKYFNSTEEASTTTST
                     VDETTIVGLLKNNYGQLLSGLGITIGLALLSFAIAIVIGIIFGMFAVSPVKALRVTSS
                     VFVDVVRGIPLMIVAAFIFWGIPNLIESMTGQQSPINDFVAGTIALSLNSGAYIAEIV
                     RGGIQAVPAGQMEASRSLGISYGTTMRKIILPQAGKIMLPNFINQFVITLKDTTIISA
                     IGLVELFQAGKIIIARNYQSFRMYAILAIIYLVVITLLTRLARKLEKGGK"
     gene            2206..2946
                     /locus_tag="AC1PIW_001813"
     CDS             2206..2946
                     /locus_tag="AC1PIW_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIP6606707.1"
                     /translation="MAQLKINVHDLHKSYGKNEVLKGITAKFYEGDVVCIIGPSGSGK
                     STFLRTMNLLETITSGQVTVDGYDLTDPKTDVDAFRSNVGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMDKSEAEKVGMELLEKVGLADKRDAMPDSLSGGQKQRVAIARALAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKDLAEQGMTMLIVTHEMGFARKVANRVIFTDGGEF
                     LEDGTPEQIFDNPQHPRLQDFLDKVLNV"
BASE COUNT          932 a          661 c          613 g          897 t
ORIGIN      
        1 tatggaaatg aagaaaaaat tactcatgtt attggcaagc attctgccag tattctttat
       61 ttttactggc attaaagctg acgatactat tgatgtcgta tttgacaatg cttacgcccc
      121 attcgagttt aaagactcag atcaaattta taaaggatta gatgttgaca tcatcaacga
      181 agtagccaag cgctcaggtt ggaccatgaa tcaaagcttc ccaggatttg atgcagctgt
      241 caacgctgta caggctggat ctgcggacgc cctaatggca ggtacaacta tcaccgaagc
      301 gcgtaagaaa gtatttactt tctcagaccc ttacttcgac accaaaatcg ttatcgctac
      361 cacaaaggca aataccatct cttcatataa ggaccttaaa ggcaagactg tcggtgtcaa
      421 aaacggtact gcagcccaaa acttcctaga agaaaacaaa gacaagtacg gatacaacgt
      481 aaaaactttt gatactggcg atttgatgta taatagcctt tctgctggtg ctgttgatgc
      541 tgtaatggac gacgaagcag ttattcaata cgctatccaa caaggtcaag acctaagtat
      601 tgacatcgaa ggcgaagcca tcggttcatt tggtttctct gtaaaaaaag gtagccaata
      661 tgaatatctt gttgaagact ttaacaaggc tttagctgag atgaagaaag atggcaccta
      721 cgaaataatc atgaacaaat ggttgggagc ttctaccact tctaccgaaa gtacagatta
      781 ctcatctcgt ttaagcttaa caggaaacgc atcagctaaa gcaacacctg tcaaatcaag
      841 ctacacaatt gtggcagatt cttcttttgc tccatttgaa taccaagatg aatcaggtaa
      901 ctatgtcggg attgacatgg aattaatcaa ggcaatcgct gaacaccaag gatttaccat
      961 tactattcaa aaccctggtt tcgacgctgc actaaatgct gttcaagctg gacaagctga
     1021 tgcagttatt gccggtatgt ctatcacaga tgctcgtaag gaaatctttg acttctcaaa
     1081 cgcctactat acttcaaaca tcctattagc tgttaaaaat ggtagtgaca tcgcttctta
     1141 cgaggactta aaagggaaaa ctgttggggc taaaaatggt actgcttcat atagtttctt
     1201 agaagaaaac aagtcaaaat atggttacac cctaaaagcc ttcgacgaag cttcaagtat
     1261 gtatgacagt ttaaactctg gttctattga tgctcttatg gatgacgaag cggttcttct
     1321 atatgccatc caacaaggtc gcaactttgc aacaccaatt cctggcgaaa aatcaggtga
     1381 gtacggattt gccgttaaaa aaggtgccaa cccagaatta attgaaatgt ttaacaacgg
     1441 cttagctgcc ctagttgaat caggcaagta tgatgaaatc cttaataagt atttcaactc
     1501 aactgaagaa gcaagcacaa caacttcaac tgttgatgag acaacgattg ttggtctttt
     1561 gaaaaacaac tacggtcaat tactctcagg tcttggtatc acaattggac ttgcccttct
     1621 atcatttgct attgcaattg tgatcggtat tatcttcggt atgtttgctg tcagcccagt
     1681 taaagcactc cgtgttactt catctgtttt tgtagatgtt gttcgtggta ttccgctcat
     1741 gattgtcgct gcattcatct tctggggtat tccaaactta atcgagtcga tgactggtca
     1801 acaatctcca atcaatgact ttgtcgctgg tacgatcgcc ctatccctca actctggtgc
     1861 ctatattgcc gagattgttc gtggcggtat tcaggcagtt ccagctggtc agatggaagc
     1921 atctcgcagt ttaggtattt cctacggaac aaccatgcgt aagattatcc tacctcaagc
     1981 tggtaaaatt atgttgccta actttatcaa ccagtttgtt attactctga aagataccac
     2041 tatcatttct gcgattggtt tggtcgaact cttccaagcg ggtaaaatca ttattgcacg
     2101 taactaccaa tccttccgta tgtatgcaat tcttgccatc atttacttgg tagttatcac
     2161 actcttgact cgcttagcac gcaaactcga aaaaggaggc aaataatggc tcaattaaaa
     2221 atcaatgtcc atgacttaca caagtcttat ggtaaaaacg aggtcctcaa aggtattact
     2281 gctaagttct atgaaggcga tgttgtttgt atcatcggtc cttctggttc aggtaaatcg
     2341 accttccttc gtacaatgaa cttacttgaa accattacaa gtggtcaagt aacagtcgat
     2401 ggatatgact taaccgaccc taagacagat gttgacgcct tccgttcaaa tgtcggaatg
     2461 gtattccaac acttcaacct cttccctcat atgtctgtac ttgacaatat cacatttgcg
     2521 ccaatcgaac acaaattgat ggacaagtct gaagctgaaa aggtcggcat ggaattattg
     2581 gaaaaagttg gattggcaga caaacgcgac gctatgccag acagcctttc aggtggtcag
     2641 aagcaacgtg ttgctatcgc tcgtgccctc gctatgaacc cagacatcat gctcttcgac
     2701 gaaccaactt ctgcccttga ccctgaaatg gttggcgatg tactcaacgt tatgaaagac
     2761 ttggctgagc aaggtatgac catgttaatc gtcacacacg agatgggctt tgcccgcaag
     2821 gttgctaatc gcgttatctt cactgatggc ggtgagttcc ttgaagatgg tacaccagag
     2881 caaatctttg ataacccaca acatccacgt ttacaagact tccttgataa ggtcttgaac
     2941 gtctaagcta aacgaaaagc cttgacttgc atcaaggttt cagattgaag aaaaagtcca
     3001 ttttggacga ttttcttcaa tcttttttct ttatgttgaa aataaaaaac tcttagaaac
     3061 gccgaaatat caatgtttct aagagtttaa tgacttgcta tct
//
LOCUS       DBNFCK010000154         3050 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.154, whole genome shotgun sequence.
ACCESSION   DBNFCK010000154 DBNFCK010000000
VERSION     DBNFCK010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.154"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            37..411
                     /locus_tag="AC1PIW_001814"
     CDS             37..411
                     /locus_tag="AC1PIW_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIP6606708.1"
                     /translation="MAWKFDNNIPIYIQISNTIKLQIVTNQLKSGDKLPTVRDLAEIA
                     GVNPNTVQRALSDLETEGFVYSVRTTGRFVTDNQELITQTRLHLAQKELENFVNNMLE
                     LGFRHDELTSQLDDYLKGVTYE"
     gene            404..1111
                     /locus_tag="AC1PIW_001815"
     CDS             404..1111
                     /locus_tag="AC1PIW_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6606709.1"
                     /translation="MSNAYTLVELQQVSKSYGGAVALNNVNLKLAAGKIIGLLGPNGS
                     GKTTMIKLINGLLQPEYGQVLINGRTPSPETKAIVSYLPDTTYLDEHMKVLEAVTFFE
                     DFYADFDKERALHLLQDLGIDLNSRMKHLSKGNKEKVQLILVMSRQAQLYVLDEPIGG
                     VDPAARDYILKTIVNNYSPTASVIISTHLISDVEQILDEVIFLHYGSVIRHGNVDDLR
                     IESGESIDELFRQDFKA"
     gene            1128..1910
                     /locus_tag="AC1PIW_001816"
     CDS             1128..1910
                     /locus_tag="AC1PIW_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6606710.1"
                     /translation="MFKKLLKYEFQSVGKWYLGIYIAAVVLSAVLGFWLQALALRTQA
                     GNTEPGGGEMVLLGTSFMTFGILIAALFLSTFVLVINRFRKNVYGRQGYLTMTLPVSS
                     HQIILSKLLASIVWYILAGITCLLSIGIILAVILAASEEAIMPEIQTFVQAIDWTILF
                     SQIVYYLIEITTGILLIYFSISVGQLFKDHRFLFAILTYIGILIVTGVFSTFFVLSNL
                     GTAYSTPFSLYPSPVLALVNIILAFAYYFGTHFIMTKKLNLQ"
     gene            complement(1954..2955)
                     /gene="add"
                     /locus_tag="AC1PIW_001817"
     CDS             complement(1954..2955)
                     /gene="add"
                     /locus_tag="AC1PIW_001817"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HIP6606711.1"
                     /translation="MLNVNELVKTELHCHLDGSLSLGVIRQLAQMAKITIPAEDEALR
                     KLVSVHGKVDSLMAYLKLFDFVRPLLQTATALELAAYDLVRQVASDKVIYIEVRFAPE
                     LSTDQDLTILEAVSAVLVGLNRGQEDFGVVAKLLVCGLKQTNTNQTKELFLAIADLAP
                     KGLVGFDFAGNEADYPTEELRDIIQFTQSLGYPMTFHAGECGCVTNVIQALELGIRRI
                     GHGTALTRNPEAIQAFVNSGATLEMCLTSNLQTGAADSIEYFPYRELVEAGANITINT
                     DNRTVSNTTLNREYQLFVEYFGTSKADFYRFNQNAIQASFASEEEKKVLLELLDQQY"
BASE COUNT          908 a          619 c          552 g          971 t
ORIGIN      
        1 acataaacac ggtgtcatcc tagaaaggaa atgattatgg cttggaaatt tgataataac
       61 atccccatct atattcaaat cagcaatact attaaattgc agattgtgac taatcaatta
      121 aaatctggag ataaacttcc cactgttcgt gacttagctg aaattgctgg cgtcaatcca
      181 aataccgttc agcgcgcttt gtctgattta gaaacagaag gatttgttta ctcggttcgc
      241 acaacaggtc gttttgtaac agataatcaa gaattgatta cacaaactcg cctacattta
      301 gcccaaaagg aactggagaa cttcgtgaac aatatgttag aattgggctt tagacacgat
      361 gaactcacta gccagttaga tgattatttg aaaggagtta cttatgagta acgcatatac
      421 tttggttgag ctacagcaag tttcaaaatc ttatggtgga gctgtcgccc taaataatgt
      481 taatttgaag ttagctgccg gaaaaatcat aggacttttg ggaccaaatg gttcgggtaa
      541 aacaaccatg attaaattga tcaacggcct gttgcagcca gaatatggtc aagtattgat
      601 caatggtcgc acaccatcgc cagaaaccaa ggcgattgta tcttatctcc cagataccac
      661 ttatcttgat gaacatatga aagtgctcga agcagttaca ttttttgaag atttttacgc
      721 tgattttgat aaggagcgtg ctctccatct tttacaagat ttgggtattg atttaaacag
      781 tcgtatgaaa catctctcca aggggaataa ggaaaaagtc cagctcattc tggtgatgag
      841 ccgtcaagca cagctgtatg ttttagatga gccaattggt ggtgttgacc ctgccgcccg
      901 agactacatt ttaaaaacca tcgtcaacaa ctattctccg actgcctctg ttattatctc
      961 tactcactta atttcagatg ttgagcaaat tttggacgag gttattttcc tacactacgg
     1021 aagcgttatt cgccatggga atgtagatga cttgcgaatt gaaagcggag aatctattga
     1081 cgaactcttc cgccaagatt ttaaggctta gttagaagga gattgctatg tttaaaaaat
     1141 tattgaaata cgaatttcaa tccgtaggta aatggtattt gggaatttat attgcagctg
     1201 tggtcctatc agcagtactt ggtttttggc tacaagcgtt ggccctccgt acacaggctg
     1261 gaaatactga acctggcggt ggagagatgg tcttattggg aacatccttt atgacttttg
     1321 gtattttaat tgccgcactc ttcttgtcaa catttgttct agtaatcaac cgttttagaa
     1381 aaaatgttta tggccgacaa ggttatctca ctatgaccct gccggtatct agccatcaga
     1441 ttatcttaag caaactactt gcctctattg tttggtatat cttggcaggt attacttgct
     1501 tactttcaat tggaatcatc cttgcagtca ttctagcagc aagcgaagaa gctattatgc
     1561 cagaaataca aacttttgtt caagctatcg actggacaat tctattcagt cagattgtct
     1621 attatttaat tgaaattacc actggtatcc ttttgatcta cttctctata tctgtcggtc
     1681 aattattcaa ggatcatcgc ttcctctttg caatcttgac ttatatcggc attctcatcg
     1741 ttactggagt atttagcact ttctttgtcc taagtaacct aggaacagct tattctacac
     1801 cattttctct ctatccaagt ccagtcttag cattagtcaa tattatccta gcttttgctt
     1861 actactttgg aacccacttc attatgacaa agaaattaaa tctacagtaa aaagctcttt
     1921 actggtctaa tcgaccggta tagagctttt ttcttaatac tgttggtcta ggagttccaa
     1981 caaaactttc ttttcttctt cgctggcaaa acttgcctga atagcatttt gattgaagcg
     2041 gtaaaaatca gctttgctag tcccaaaata ttctacaaat aactgatatt ctctattaag
     2101 tgtagtatta gaaacggtac gattatctgt gttgattgtg atgtttgctc ctgcctcaac
     2161 tagctcacga tatggaaaat attcaatcga atcagctgca ccagtttgaa gattcgatgt
     2221 taggcacatt tcaagtgttg ctccgctatt gacaaaagct tggatagctt caggattgcg
     2281 agtaagtgct gtgccatgac caatccttct aatcccaagt tcaagagctt gaatcacatt
     2341 ggtcacacat ccacattctc cggcatggaa agtcatggga taacctaaag actgagtaaa
     2401 ttgaataata tcccgaagtt cttccgtagg atagtctgct tcatttcctg cgaaatcaaa
     2461 tcccacaagt ccttttggtg ccaaatcagc aatagccaaa aagagttcct tcgtttgatt
     2521 cgtattcgtt tgctttaaac cacaaaccag aagtttagca accactccaa aatcttcctg
     2581 ccccctgttt agtccaacta aaactgctga gaccgcttct aaaatagtca agtcttgatc
     2641 cgtcgacagt tctggtgcaa aacgaacctc aatgtaaatg accttatcac ttgcaacttg
     2701 tcgtacaaga tcataggctg ccaattctaa agctgttgca gtttgtaaga gtgggcgaac
     2761 aaaatcaaat aactttaaat aagccattaa actatcaact tttccatgaa cactaactaa
     2821 ttttcttaat gcttcatctt ctgcaggaat agtgatttta gccatctgtg ccaattgtcg
     2881 aatgactccg agggacaatg agccatccaa atgacaatgc aattctgttt tgaccaattc
     2941 atttacattc aacactatta acacaacctc cgtctggtat attgttaact atcatatcat
     3001 atttttgaag aaaaacaaga attttaccat aaaacctgaa ctttcatttg
//
LOCUS       DBNFCK010000155         2981 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.155, whole genome shotgun sequence.
ACCESSION   DBNFCK010000155 DBNFCK010000000
VERSION     DBNFCK010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.155"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..466)
                     /locus_tag="AC1PIW_001818"
     CDS             complement(<1..466)
                     /locus_tag="AC1PIW_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HIP6606712.1"
                     /translation="MNKIESRHQLILSLIMEKKIHTQQELQELLEVNGVSVTQSTLSR
                     DIKMLNLVKVNEDDSSHYVINPIAPTRWEKRLRLYMEDALVMLKPIQHQVVLKTLPGL
                     ANSFGSILDAMEIPQIVATVCGDDVCLIICEDVEGAQACFEHLKQFTPPFFFS"
     gene            complement(515..1543)
                     /gene="queA"
                     /locus_tag="AC1PIW_001819"
     CDS             complement(515..1543)
                     /gene="queA"
                     /locus_tag="AC1PIW_001819"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HIP6606713.1"
                     /translation="MNTADFDFDLPEELIAQVPLEKRDSSKLLILDREKRSMVDSHFD
                     HIIDQLNPGDALVMNNTRVLPARLYGYKPETMGHVELLLLKNTQGDQWEVLAKPAKRL
                     KVGTTVAFGDGRLTATIVEELEHGGRIVEFSYEGIFLEVLESLGEMPLPPYIHEKLED
                     RERYQTVYAKENGSAAAPTAGLHFTQELLEKIEAKGVKLVYLTLHVGLGTFRPVSVDN
                     VDEHEMHSEFYNLSAEAAQTLNQVKDSGGRIVAVGTTSIRTLETIGNKFDGRLQADSG
                     WTNIFIKPGYTFRIVDAFSTNFHLPKSTLVMLVSAFAGREFTLDAYKHAVEERYRFFS
                     FGDAMFIQ"
     gene            1889..2593
                     /locus_tag="AC1PIW_001820"
     CDS             1889..2593
                     /locus_tag="AC1PIW_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HIP6606714.1"
                     /translation="MKIHVVNNQVEGATVALDILREKLNGGTKVLGLATGSSPLEFYR
                     LIRESDLDFSDVTSVNLDEYVGLGEESDQSYIHFMKENLFNTKPFKQSYLPNGLATDV
                     VAETERYNKILAEHPVDFQILGIGRNGHIGFNEPGAPFDGQTHLVELAPSTIEANARF
                     FDNPEDVPKQAISMGIANIMAAKTIVLMAYGQEKADAIKATVEGAVTEDVPASVLQNH
                     DNVILILDQAAASKLV"
BASE COUNT          917 a          550 c          651 g          863 t
ORIGIN      
        1 tactaaagaa aaatggtgga gtaaactgtt tcaagtgctc gaagcaagct tgtgccccct
       61 cgacatcttc acaaatgatg agacagacat cgtcaccgca gacggtcgct acaatttgag
      121 gaatttccat ggcatccaaa atagaaccga atgagttggc caatccaggt agggtcttga
      181 gcaccacttg atgttggata ggttttagca tgaccaaagc atcttccata tagagtcgca
      241 gtcgtttttc ccagcgagta ggagcaatgg ggttgatgac atagtgagaa gaatcatctt
      301 cgttgacttt gacaagattg agcatcttga tgtcacggga gagggtagat tgggtgacgg
      361 agactccgtt gacctctagt aattcttgca attcctgctg ggtatgtatt ttcttctcca
      421 tgattagaga taggatcagc tgatggcggc tttcaatctt gttcataggg ccctccaata
      481 aaatcagaat tcagaataat ttggtgataa cagcctattg aatgaacatt gcatccccaa
      541 aactaaagaa gcggtagcgt tcttcaacag catgtttata ggcatctaag gtaaattctc
      601 tacctgcaaa ggcagaaact agcataacca aagtagattt tggcaggtgg aaattggttg
      661 agaaggcatc aacgattcgg aaggtgtagc caggtttgat gaagatattt gtccaaccag
      721 aatcagcttg caaacggcca tcaaatttat taccgatggt ttcgagggtg cggatggatg
      781 tcgtgccgac tgcgacgata cgaccaccgc tatctttgac ctgattgagg gtttgagcag
      841 cttctgctga taggttatag aattccgagt gcatttcatg ctcatcaacg ttatcaacag
      901 agactggtcg gaaggtccca agtccaacgt gaagagtcaa atagactagc ttcactcctt
      961 tagcttcgat tttttcgagt aactcttggg tgaaatgtag tccagcagtt ggggctgcgg
     1021 cagaaccgtt ttccttggca taaactgttt gatagcgttc acggtcttct aatttctcat
     1081 gaatgtaagg tggaagtggc atttcgccta gactttccaa gacttcaagg aagatacctt
     1141 cataggagaa ttcaacaatt cttccaccgt gctctaattc ttctacgata gtagcagtta
     1201 atcgtccgtc accaaaagcg actgtggtac cgaccttcag acgttttgct ggtttggcaa
     1261 gaacttccca ctgatcacct tgggtatttt tcaaaagcaa aagctcaaca tgtcccatag
     1321 tttcaggctt gtagccatag agacgggcag gcagaacacg cgtattgttc atgaccagag
     1381 catcaccggg attcaactgg tcgatgatat ggtcaaagtg actgtcaacc atgctccgtt
     1441 tttcgcggtc gaggatcaat agtttagagc tgtcgcgctt ttcaaggggt acttgagcga
     1501 taagctcttc gggtaaatca aaatcaaaat ctgcagtatt catattcact ccttagacta
     1561 aaataaaata taggtaaaat aaatcaatag ggcaaatggc aaagccacca atagaccacc
     1621 aatcaggtag aaaataaact ttaggacctt tgatttagaa ataaaatagc cagtaaggca
     1681 gaacagaata ccaataaaaa ataagtaaaa aagcacatct gtcataaatg tattatatca
     1741 tagttagaca cttaagtctt gatggatggg aagagaatta aaattattcg tgctgatgta
     1801 atactctata agtataaatt cttgacaaaa attggtctat accatataat aaagaaaaac
     1861 aaaatggtat agaccaaaga ggtgtgttat gaaaattcat gtagtaaaca atcaagttga
     1921 aggagcaact gtagcattag acattttgag agaaaaattg aacggaggta ccaaggtatt
     1981 aggtttggca acaggctcca gtccgttaga attttacaga ttgattcgtg agtcggattt
     2041 agacttttcg gatgtgacat ctgtaaactt ggatgagtat gttggtttag gtgaggagag
     2101 cgaccaatcc tatattcatt ttatgaagga aaatcttttc aataccaaac cgtttaaaca
     2161 atcttattta ccgaatggtt tggcgacaga tgtagttgct gaaacagaac gttacaataa
     2221 aattttggct gaacatccgg ttgatttcca aattttgggt atcggtcgca acggccatat
     2281 cggtttcaac gaaccaggtg ctccatttga tggtcagacc catctggttg aattggcgcc
     2341 atcaaccatt gaagccaacg cccgcttctt tgacaatcca gaagatgtac caaaacaagc
     2401 tatctccatg ggcatcgcca acattatggc agccaagacc attgttctca tggcctatgg
     2461 tcaggaaaaa gctgacgcca ttaaggcgac tgtggaaggg gcagtgacag aagatgtccc
     2521 agcaagcgtc ctccaaaacc atgacaatgt catcctcatc ctcgaccaag cagcagcgag
     2581 taaattagtg tagtatgttg taaacgatta ttcttagaca taaagttggt tgtaacagta
     2641 caataagtat aatttctgaa tgaatctagg ttctttttcc attatgaatt tgacaaaaat
     2701 tgtgaaaagg catgtaactt atatttttaa gggcatgcct tttgctattc tgataatcca
     2761 tatcagttgt tatatatttg aatggattat atataacatt aagttggatg tgagagaaaa
     2821 tttcaacaag aggtattgcc atcctttatc atgttgaaaa ttgataggga taagtgaaat
     2881 gggcgaaata gtatcatttt ctctacgttt tgtataacgt tttcaatttt ttcaggtagt
     2941 tttctggttt ttatataatc tttttctaaa gttggttttt g
//
LOCUS       DBNFCK010000156         2948 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.156, whole genome shotgun sequence.
ACCESSION   DBNFCK010000156 DBNFCK010000000
VERSION     DBNFCK010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.156"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            130..1275
                     /gene="nagA"
                     /locus_tag="AC1PIW_001821"
     CDS             130..1275
                     /gene="nagA"
                     /locus_tag="AC1PIW_001821"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HIP6606715.1"
                     /translation="MTVFIKAKSIILPESEVESAYLEITDKGQFGRIVIEKPEGEIVD
                     YSDFHLAPGLVDTHIHGYASHDVMDNDFEGIKVISEGLLSCGVTSWLPTTLTDTTENL
                     DAVCETIGTYAGQETGAKIQGIFLEGPFFTEKYKGAQNPKYMSDPSIEKLDNWHSLSK
                     GLVNKIAIAPEREGVKEFIEFANSKAIHTALAHSDATYAQAEAAVEAGANIFVHVYNG
                     MSGLHHREPGMVGAALNLKNVYAEMICDGHHVHPAAAEIVVKARGAEETVLITDCMRA
                     GGMGEGESRLGEFEVVVKDGTARLKESGSLAGSILELIEAVQNVVKWGLVSLPDALRM
                     ASLAPARSVNIDHICGRIAEGRAADFIVVDDAGRLQATYLDGVKRFG"
     gene            1298..2299
                     /gene="lacD"
                     /locus_tag="AC1PIW_001822"
     CDS             1298..2299
                     /gene="lacD"
                     /locus_tag="AC1PIW_001822"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HIP6606716.1"
                     /translation="MTKLQLSQAKFNHMTRLSNSDQVIAALAIDQRGALKRLLAAAAG
                     GGEFGDDILIDFKKVISSDLTPYASSILLDAEYGVPASELRHADCGFIAAYEKTGYDA
                     STPGRLPDLLPNWSAKRIKELGADAVKILLYYDVDDKPEINDIKHAWVERIGSECIAE
                     DIPYFVEILTYDASDMDVTSREYAALKPHKVNGAMREFSKSRYNADVLKVEVPVNMNF
                     VEGYTDEEPVYTVEEAKAFFKEQTDSTHLPFIYLSAGVSAKLFQETLVFAKEAGSSFN
                     GVLCGRATWKDAVAIFASEGEEAAKAWLADQGRRNVEELNEVLAITARPWTEKVEVK"
BASE COUNT          881 a          538 c          679 g          850 t
ORIGIN      
        1 cttctcacag catccatttc ttataaagtt atggtaatta cttgacattt tctaatagaa
       61 gttctacaat atatttggta tatggttata accagtttga taagtatata tatcaataaa
      121 ggagataata tgacagtatt tataaaagca aaatctatta ttttaccaga gagtgaggtc
      181 gaatcagcct atttggaaat tactgacaag ggtcagtttg gacggattgt gattgaaaag
      241 cctgaagggg agattgttga ttattctgac tttcatttag caccagggct agttgataca
      301 catatccacg gctacgcttc tcacgatgtt atggacaatg attttgaagg gatcaaggta
      361 atttcagaag gtctcttgtc ttgtggggtg acctcttggc ttccaacgac attaacagat
      421 acgactgaaa atttagatgc agtttgtgaa acgattggga cttatgcagg gcaggaaaca
      481 ggcgccaaga ttcaaggtat tttcttagaa ggtcctttct ttacagaaaa atacaaggga
      541 gcacaaaatc ctaagtacat gagcgatcca tccattgaaa agttagacaa ttggcacagc
      601 ttatctaaag gtttagtcaa taaaattgct attgctccag aacgtgaagg cgtcaaagaa
      661 ttcatcgagt ttgctaacag caaggcaatt catactgcct tggctcacag cgatgcgacc
      721 tatgcgcaag cggaagcggc agtagaagct ggtgccaata tcttcgtcca tgtttacaat
      781 gggatgagtg ggcttcatca ccgtgaacca ggtatggtcg gtgctgcttt gaatttaaaa
      841 aatgtctatg cggaaatgat ttgtgatgga caccatgttc atccagctgc ggcagaaatc
      901 gtggtcaaag cacgtggagc tgaggaaact gttttgatta cggactgtat gcgagctggc
      961 ggtatgggtg aaggggagtc ccgcctcggt gaatttgaag ttgttgtaaa agatggaact
     1021 gctcgtttaa aagaaagtgg tagtttggca ggttctatct tagaattgat tgaagcagtg
     1081 cagaatgttg tgaaatgggg cttggtatcc ctacctgatg cccttcgtat ggcttctctg
     1141 gcaccagctc gctctgtcaa tattgaccat atttgtggtc ggattgcaga aggccgagct
     1201 gcagacttta tcgtcgtaga tgatgcagga cgcttacaag cgacctatct agatggcgtg
     1261 aaacgatttg gttaataaaa tccttaggag gaaactaatg acaaaattac aactttctca
     1321 ggctaaattc aatcacatga ctcgcttgtc aaacagcgat caggtgattg cggctttggc
     1381 gattgaccaa cggggggctt tgaaacgctt gcttgctgct gcagcaggtg gtggcgaatt
     1441 tggagatgac attttgattg acttcaagaa agtcatctca agcgatttga ctccttatgc
     1501 aagctccatt ctcttggatg cggaatacgg cgttccggct tcagaattgc gccatgcaga
     1561 ttgcggtttc attgcagctt atgaaaaaac aggttacgat gcgtctacac caggtcgttt
     1621 gccagacctt ttgccaaatt ggtcagccaa acgtatcaaa gaattgggtg cagatgccgt
     1681 aaaaatcttg ctttactatg atgtagatga caagccagaa atcaacgaca ttaagcatgc
     1741 ttgggtagaa cgcattggta gtgagtgtat cgcagaagat attccttact tcgtcgaaat
     1801 cttgacttac gatgccagcg atatggatgt gacttctcgt gaatacgcag ccctcaaacc
     1861 acacaaggtc aatggtgcca tgcgtgaatt tagtaagtca cgttataatg ccgacgtgct
     1921 caaggttgaa gtacctgtca atatgaactt tgtagaaggt tacacagatg aagagccagt
     1981 ctatacagtt gaggaagcta aggccttctt caaggaacaa acagatagca ctcacttgcc
     2041 attcatttat ttgagcgctg gcgtatctgc taaacttttc caagaaaccc ttgtctttgc
     2101 caaagaagct ggttcaagct ttaatggtgt tctttgtggt cgggcgactt ggaaggatgc
     2161 agtagccatc tttgcaagcg agggtgaaga agcagccaaa gcttggttgg cggatcaagg
     2221 ccgccgcaac gtagaagaac taaacgaggt tctagcaatt acagcgcgtc cttggactga
     2281 aaaagttgaa gtaaaataat tgttttagta gggtctgggc ttaggttcag actttttgta
     2341 acatcaaata tagtcattta tactcttcga aaatcaaaat cagtcgatgt tgacctggtt
     2401 ttgcaatccg ctcttatatc tctcaccttg aacagtccat ttactgtgca agcaatccca
     2461 gaaacagtta tagctgcggt tgttgacgca aaccttgttc cccttatttc caacctctga
     2521 ctatttgtga aatagttgga gcaagtacta aaagtaaact aaaaggctat ataaacaaga
     2581 tgacaggtcg ttttatagct aataatatag gggtctattt ttagaaaaat tcgaaaaaac
     2641 ttgaaaaatc tgaaaattta cgcaattccc tattgacaaa taatttgaaa acagttatga
     2701 tagttacaaa taaatgagaa agggaaacag agatgttaca aatgtgtcaa aatcattcag
     2761 ctaatcatac ttatttctac ggctatttta gatagtgttt gtagacatgg taaacatgga
     2821 tgcaaacacg ggaacgtaaa gtttgtatct atgtggctgt agctattttg acatgtgcct
     2881 catagatgat acatctaaat ggcacggata attcattctg ttttcctata cagatgaaat
     2941 ataaggac
//
LOCUS       DBNFCK010000157         2941 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.157, whole genome shotgun sequence.
ACCESSION   DBNFCK010000157 DBNFCK010000000
VERSION     DBNFCK010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.157"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..1808
                     /gene="ftsH"
                     /locus_tag="AC1PIW_001823"
     CDS             <1..1808
                     /gene="ftsH"
                     /locus_tag="AC1PIW_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIP6606717.1"
                     /translation="EITYQPNSSVIEITGKYKTEQEAKDELASSIKLFQVSSKVKYKN
                     FKSLILPSETNLSELQALANENGVKVAIKPESSNGLWLNIIFNLLPLVIAGVFFMMMM
                     NQGGGGARGAMNFGRNKAKALEQSNIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTK
                     LGARIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLF
                     EDAKKAAPAIIFIDEIDAVGRQRGVGMGGGNDEREQTLNQLLIEMDGFEGNEGIIVIA
                     ATNRSDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAADVDLKLVAQQT
                     PGFVGADLENVLNEAALVAARRNKTVIDASDIDEAEDRVIAGPSKKDRQVSAKEREIV
                     AYHEAGHTIVGLVLSNAREVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGL
                     MGGRVAEEIIFNTQTTGASNDFEQATQMARAMVAEYGMSDKMGPMQYEGSHAMFGGQT
                     TQKHISEQTAYELDNEVRDLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSVQIK
                     SLYETGKMPENIERDDEDVHPLSYEEVKEKINTKE"
     gene            2146..2616
                     /locus_tag="AC1PIW_001824"
     CDS             2146..2616
                     /locus_tag="AC1PIW_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIP6606718.1"
                     /translation="MEFEKVYASVKGIVNKARKEFYIKLWDRDDWEQEGMMTLFELLE
                     AQPWLVDEQVQLYCYFKVKFRNRIKDRIRKQESQKRKFDRMPHEDIYELSHAIQSPGL
                     INDELLMLRGALRDYRKNLSNDQLDKYEKLISGQCFNGRREMIRDLQIHLKDFR"
BASE COUNT         1020 a          500 c          637 g          784 t
ORIGIN      
        1 ctgaaattac ttatcaacca aattcaagtg ttattgaaat tactggtaag tacaaaacag
       61 aacaggaagc taaagatgag ttagcttcat ctattaagtt attccaagtt tctagtaaag
      121 ttaagtataa aaactttaag tcgctaattt taccgtcaga aacgaatttg tcagagctac
      181 aagctcttgc aaatgaaaat ggagtaaagg ttgctattaa acctgaaagt tctaatggac
      241 tatggttgaa cattatcttt aatttgttgc cacttgttat tgctggtgta ttcttcatga
      301 tgatgatgaa ccagggtggt ggtggagcac gtggtgcaat gaactttgga cggaataaag
      361 ctaaagcttt ggaacaaagt aacatcaagg tacgtttctc agatgtagca ggtgcagagg
      421 aagaaaaaca agaattagtt gaagttgttg aatttttgaa ggatccaaaa cgctttacta
      481 aacttggagc acgtattcca gcaggtgtgc tactagaggg tcctccagga accggtaaaa
      541 ccttgcttgc caaagcagta gcgggtgaag cgggtgtacc attcttctcc atttctggtt
      601 cagattttgt tgaaatgttt gttggtgtcg gtgccagccg tgttcgttca ttatttgaag
      661 atgcaaaaaa agcagcacca gccattattt tcatcgatga aatcgatgct gtcggtcgcc
      721 aacgtggtgt cggcatgggt ggcggtaacg atgaacgtga acaaaccctc aaccaactct
      781 taattgaaat ggatggtttt gaagggaacg aagggattat tgtcattgcc gcaaccaacc
      841 gtagtgatgt tttagaccct gcccttcttc gtccaggtcg ttttgaccgt aaggtattgg
      901 ttggtcgtcc ggatgttaaa ggacgtgaag caatccttaa agttcatgcg aagaataaac
      961 cactagcagc agatgtagat ttgaaattag tagcacaaca aacaccagga tttgttggtg
     1021 cagatttaga gaacgttctg aacgaagcag cccttgtagc tgcacgtcga aacaagactg
     1081 tcattgatgc ttcagatatt gacgaagcag aagatcgtgt tattgcaggt ccatctaaga
     1141 aagaccgtca agtttcagcc aaagaacgcg aaatcgttgc ctaccatgaa gctggtcata
     1201 cgattgtcgg ccttgtcttg tcaaatgcac gtgaagttca caaagtaaca attgttcccc
     1261 gcggtcgcgc aggtggatac atgattgccc ttcctaaaga agatcaaatg cttctttcta
     1321 aagaagatat gaaggagcaa ttggctgggc ttatgggtgg tcgtgttgcg gaagaaatta
     1381 tttttaatac acagaccaca ggagcatcaa acgacttcga gcaagctaca cagatggcgc
     1441 gtgctatggt tgctgaatat ggtatgagtg acaaaatggg accaatgcag tatgaaggca
     1501 gtcatgcaat gtttggtgga caaacaactc aaaaacatat ttctgagcaa actgcatatg
     1561 agttagataa tgaagtacgt gatttgttaa atgaagctcg caacaaagcg gctgaaatta
     1621 ttcagtctaa tcgcgaaact cataaactaa tcgcagaagc attacttaaa tacgaaaccc
     1681 tagacagtgt tcagatcaaa tctctctatg aaacaggcaa aatgccagag aatattgaac
     1741 gtgatgatga agatgttcat cccctttctt atgaagaggt aaaagagaaa attaatacga
     1801 aagagtagta taaaaatact cttggcaaga cattccctca ctctcaacca ctaaaataac
     1861 cacaaagcct tcgctaagta cataatcttg gcgagggctt ttttacatga tacacatcag
     1921 aaacagggca tttacaagaa aaacgaatca atagttattt atcacaatct tagaactata
     1981 ccaaatcact cccttcagaa gcagaatgca atatttgcca tagagtatca atagcaaatg
     2041 tactttaaaa aagaaaaatc aaataaagta tgctagacaa aaatgtcctg tagaaagaca
     2101 gcacttttcg ataaacttaa accatcaaca aaaggagatt aagtaatgga attcgaaaaa
     2161 gtgtacgcaa gcgtcaaagg tattgtaaac aaggctcgaa aagagtttta cattaaacta
     2221 tgggatcgag atgattggga acaagaagga atgatgacct tgtttgaatt gttggaagct
     2281 caaccgtggc tagttgatga acaagttcaa ttatattgtt attttaaagt caagttcaga
     2341 aatcgaatca aagatcgtat ccgcaaacag gaaagtcaaa aacgcaagtt tgaccgtatg
     2401 ccacatgaag atatttacga attatctcac gcaatacaat caccgggatt gataaacgat
     2461 gaattattaa tgttgagagg tgccttgaga gattatcgaa aaaatctgag caatgatcaa
     2521 cttgataaat acgaaaaatt aattagcgga caatgtttta atggtcgccg tgaaatgata
     2581 cgtgatttac aaattcattt gaaagacttt cgctaaagct agttactaac atcacgttaa
     2641 tcgaattaag aaattgggca gaagcaccat aaaaagttct gttcagtttc tttttagata
     2701 ggttatagca caggaagaag gaataggaga aaaaacaaag tatctacata gaactttcag
     2761 tgtaaaaaat cccaaaaaac cggttgacaa ttgccaaagt agatgataga ataaatgagt
     2821 tgtctcttga gggactagtt aacagaggaa cgaaaaaagt ttcgaaaaag tgttgacaag
     2881 accatcagaa gatgatagac taatagagtt gtctcttgag ggactggtta acagaggaac
     2941 g
//
LOCUS       DBNFCK010000158         2859 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.158, whole genome shotgun sequence.
ACCESSION   DBNFCK010000158 DBNFCK010000000
VERSION     DBNFCK010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.158"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..197
                     /locus_tag="AC1PIW_001825"
     CDS             <1..197
                     /locus_tag="AC1PIW_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIP6606719.1"
                     /translation="INSQLLNYPGFSEKVNNLVDNQLQFIPSKNNLDIEFLSFNIFRG
                     AIGAASLAVYNHFILENNSN"
     gene            complement(480..1118)
                     /locus_tag="AC1PIW_001826"
     CDS             complement(480..1118)
                     /locus_tag="AC1PIW_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HIP6606720.1"
                     /translation="MRWGIVVEQLTVLYQNLKRRYGEFHWWNDENPIKDLVSMILIQQ
                     TTEANAKRALEQLEGRLTIHSLLEMPVEDLQECIRPAGFFKQKSLYIRSVVEWANQFD
                     GDFSRLDRVETAVLRKELLSLKGVGNETADVILLYLCRRSVFVADQYALRLFNRLGLS
                     QSQDYLSLRQEFTEQIKDWSVKDAQELHALIDEHGKQFRLTKGQLDESWLMI"
     gene            complement(1166..2086)
                     /locus_tag="AC1PIW_001827"
     CDS             complement(1166..2086)
                     /locus_tag="AC1PIW_001827"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008443 - phosphofructokinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HIP6606721.1"
                     /translation="MIHLICPNPALDRTLLVEKIEKNIPLRPTEVRDYPGGKSFNVAY
                     ALRENGVTDYTIHTILGGQIGRYIQELNADGGNALRVVENSQNTRTCNIYVETSTGDV
                     VLFYEKGFELTEDLLNQFTQQIENSLQAGDILVFSGSLMKGMSDDYIQRFIEKYPEVL
                     TIVDTSGPALRAAYQARPALIKINNEELKDIYPELDEESPEAILGILKEVTPHENIII
                     TMGDKGSLAKIGQRFFRIQSPKKETRNPIAAGDFYLGLLVKGISQGQAPEIFLKEAAA
                     FATANCLNYFPEVEAEQFEAIVDTIVLEEL"
     gene            complement(2188..2814)
                     /locus_tag="AC1PIW_001828"
     CDS             complement(2188..2814)
                     /locus_tag="AC1PIW_001828"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIP6606722.1"
                     /translation="MKIAIIAANGQAGQTIAKEAVERGHQVTAIVRSENKSVAQEVIQ
                     KDAFALTKEDLAGFDVVVNAFGAWTPETLPDHARLAEHLATILAGSPTRLLVVGGAGS
                     LYLDESQTAMLKDTPDFPAEYLPIAEAMGTGLDLYRQATAVNWTYISPAADFDAEGQK
                     SGAYTLAGEVFQVNAQGESYISYADYAVALVDIAEKGGYQQERISVLA"
BASE COUNT          816 a          660 c          514 g          869 t
ORIGIN      
        1 ttattaatag ccagttatta aattatccag gtttttcaga aaaagttaat aatcttgttg
       61 acaatcaatt gcaatttatt ccaagtaaaa ataatttaga tattgaattc ttatctttta
      121 atatatttcg aggtgcaatt ggagcagcat ccttagcagt ctataatcat tttatcttgg
      181 aaaataactc caattaatat aaaaaaagag caaccaactg aaccgctggt tttactcttt
      241 tatagtcgtt tcgtttgctt ttagtacaag gcaacgatat gcaggctact tcaactgtcc
      301 agtggagtgt ttaaactcgg aaataaggaa atgagacttc ctcgcccttc agtaataaaa
      361 aatacactaa ttgatactca atgaaaatca aaatcaagct aggcgatgca aatgcagata
      421 gaactgaggt tcatcaaatc atgtcaacaa cgtctgattt tgattttcga agagtatgac
      481 tatatcatca accatgattc atcaagctgc ccctttgtta acctgaactg cttgccatgt
      541 tcatcaatca gtgcatgaag ctcctgagcg tcttttaccg accaatcttt tatctgctct
      601 gtaaattcct gtctcaaact caaataatcc tgagactggc taagtcctaa acgattaaat
      661 agccgaagag catattgatc cgccacaaaa acacttcttc gacacaaata gagcaaaata
      721 acatccgcag tttcattgcc aacccccttc aagctcagca gctcttttcg gagtactgct
      781 gtctcaacac gatctaggcg agaaaaatct ccatcgaact gattggccca ctcaaccact
      841 gaccgaatgt agagtgattt ttgcttgaag aatcctgctg ggcgaatgca ttcttgtaaa
      901 tcctccacag gcatctctaa aagcgaatga attgtcaacc gtccctccaa ctgttctaaa
      961 gcacgcttgg catttgcttc cgttgtttgc tgaataagga tcatagatac caaatccttt
     1021 ataggatttt catcattcca ccagtgaaat tcaccatatc gccgttttaa attttggtaa
     1081 agaaccgtca actgttccac tactatcccc catctcacct tgtttctcct tgccattata
     1141 gcacgatgag taaagggata caatactaca gttcctctag tactattgta tctacaatcg
     1201 cttcaaactg ctctgcttca acctctggaa aataattcaa acaattggct gttgcaaagg
     1261 ctgccgcttc tttcaaaaag atttctgggg cttgaccttg actgattcct ttgactagca
     1321 agcccaggta gaaatcccct gcggcaatag gattacgcgt ttctttcttt ggagactgaa
     1381 tacggaagaa gcgctgacca atcttagcta gactcccctt gtctcccatg gtaatgatga
     1441 tattctcatg aggagtcact tctttcagga tacctaaaat agcttcagga ctttcttcat
     1501 ccagctctgg ataaatatct ttcagctctt cattattgat tttgataagg gccggtctag
     1561 cttgataagc cgcacgtaaa gctggtccac tggtgtccac tatggtcaga acctctgggt
     1621 atttttcaat aaaccgctga atataatcat ccgacatccc cttcatcaga ctacctgaaa
     1681 agactaggat gtcgccagct tgcaggctat tttcaatctg ctgagtgaat tggttgagca
     1741 aatcctctgt tagttcaaaa cctttttcat aaaatagtac tacatcgcct gtactggttt
     1801 caacatagat attacatgtc cgagtattct ggctattttc aaccacgcgc agggcatttc
     1861 caccatcagc attaagctcc tgaatatagc gaccaatctg cccacctaaa atggtatgga
     1921 tagtgtagtc tgtcactcca ttttctcgaa gtgcataggc tacgttgaaa ctttttccac
     1981 caggataatc cctcacttcc gtcggtctca ggggaatatt cttttcaatc ttttccacaa
     2041 gaagcgtgcg gtctagggct ggattgggac aaatcaagtg aatcatgggg ttctcctttt
     2101 ctttttattt atattattat actatatgca agtaaaaaat aaaaagataa ttcctcgatg
     2161 gagctatctt ttagaccatt taatttttta tgcaagtacg gaaattcgtt cctgctggta
     2221 gccacctttt tccgcaatgt caaccaaagc cacagcatag tcggcataac taatatagct
     2281 ttcaccttgg gcattgactt ggaaaacctc gccagccaaa gtgtaggcac ctgatttttg
     2341 accttctgca tcaaaatctg ctgctgggct gatataggtc cagttgaccg ctgtcgcctg
     2401 acgatagagg tctagaccag tacccatagc ctcagcgatt ggcaggtatt ccgcagggaa
     2461 atctggtgta tctttcagca tagctgtctg gctctcgtct aaataaagac tgcctgcacc
     2521 accaactact aaaagacgag ttggactacc agcaagtatg gtcgctagat gctcagcaag
     2581 acgggcgtga tccggcaagg tttctggtgt ccaagcacca aaagcattaa ctactacatc
     2641 aaagcctgcc aaatcttctt tggtaagagc aaaagcatcc ttctggatta cttcttgggc
     2701 tacgctcttg ttttcagaac ggacaatagc cgtcacctga tgaccacgtt ctactgcttc
     2761 ctttgcaata gtttgacctg cttgaccatt cgctgcaata atagcaattt tcatattgat
     2821 acctcacttg ttttgttgta acaactttat ttacaacgt
//
LOCUS       DBNFCK010000159         2774 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.159, whole genome shotgun sequence.
ACCESSION   DBNFCK010000159 DBNFCK010000000
VERSION     DBNFCK010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.159"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(51..2702)
                     /locus_tag="AC1PIW_001829"
     CDS             complement(51..2702)
                     /locus_tag="AC1PIW_001829"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIP6606723.1"
                     /translation="MSKELSPKYNPAEVEAGRYQTWLDQDVFKPSGDAEAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLREQG
                     VTRYDLGREKFLDKVWEWKDEYAATIREQWGKLGLSLDYQRDRFTLDEGLSKAVRKVF
                     VELYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALQVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIVNKPIPIVADEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAGEFAGMDRFEARKATVAKLQELGALV
                     EIENRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTADKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNESGEMYVGEEAPAGDGWVQDEDVLDTWFSS
                     ALWPFSTMGWPDENAADFQRYFPTSTLVTGYDIIPFWVSRMIFQGIEFTGKSPFKNAL
                     IHGLIRDEEGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDAARENVAKVAAGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEAEKVMTRSVLLYTLDQI
                     LRLLHPIMPFVTEEIFAQYAEGSIVVAAYPVVNPAFENAEAHKGVESLKDLIRSVRNS
                     RAEVNVAPSKPITILIKTADSELETFFKANENYIRRFTNPEQLEISSSIAAPELAMSA
                     VITGAEIFLPLADLLNVEEELARLDKELVKWQKELDMVGKKLSNERFIANAKPEVVEK
                     EKEKQADYQAKYDATVARIEEMKKLVK"
BASE COUNT          676 a          688 c          636 g          774 t
ORIGIN      
        1 tataccacaa aacccagcca ttttaggctg ggcattttat cgtattctac ctacttaacc
       61 aacttcttca tctcttcaat ccgcgctact gtcgcatcat attttgcttg gtagtcggct
      121 tgtttttctt tttctttttc aacgacttct ggtttagcgt tggcaatgaa gcgttcgttg
      181 ctgagttttt tgccgaccat gtctagttct ttttgccact tgacgagttc tttgtctaga
      241 cgagccaact cttcttcgac gttaagaagg tctgctagtg gcaagaagat ttcagcaccg
      301 gtgataacgg ctgacatggc tagttctggt gcagcgatgc ttgagctgat ttccaactgc
      361 tctgggttgg taaagcggcg gatgtagttt tcatttgcct tgaagaaggt ttcaagctcg
      421 ctatctgccg tcttaatcaa aatggtaatc ggcttagaag gagcaacatt gacttctgct
      481 ctgctatttc gcaccgaacg gatcaagtct ttaagacttt ccactccctt gtgggcttcc
      541 gcattttcaa atgctgggtt gacaacaggg taggccgcta ccacgataga accctctgcg
      601 tactgggcga aaatctcttc cgtcacaaac ggcatgattg ggtggaggag gcgaaggatt
      661 tggtccagcg tgtagagaag gacagagcgg gtcatgactt tctcagcctc gttgtcgctg
      721 taaaggacct ccttggtcag ttcgacatac cagttggcga actcttccca gatgaagttg
      781 tagaggatgt gaccagccac accaaactcg aacttgtcaa agttttcagt gactttggca
      841 atggtttcgt tgaggttgtg gagaatccag cggtccgtca cgttgccagc ctcgccagct
      901 gcgactttgg caacattctc acgcgccgca tccaaggtca aaccttcatt gttcatgagg
      961 atatagcggg aaatgttcca aatcttgttg atgaagttcc aagatgcgtc catcttctca
     1021 taagagaagc gaacatcttg accaggggca gagccgtttg acaagaacca acgcaaagcg
     1081 tccgcaccgt atttctcgat aacatccatc gggtcaatcc cgtttccgag cgacttggac
     1141 atcttgcgac cttcttcgtc acggataagt ccgtgaatca gagcattctt aaatgggctc
     1201 ttgcctgtaa attcaattcc ttggaaaatc atacgagaca cccagaatgg aataatatca
     1261 tagcccgtta ccaaggttga ggttgggaag taacgctgga agtccgccgc gttttcgtca
     1321 ggccagccca tggttgaaaa tggccaaagg gcagagctga accaggtatc taggacatcc
     1381 tcatcctgta cccaaccgtc acctgctgga gcctcttctc cgacgtacat ttcgccagat
     1441 tcgttatacc atgctggaat ctgatgaccc caccaaagct gacgagagat aacccagtcg
     1501 tgtacatttt ccatccattg taggaaagta tcgttgaagc gtggtgggta aaattctacc
     1561 ttgtcagctg tatcttggtt ggcaatggca ttcttggcca attggtccat cttaacaaac
     1621 cattgagttg acaagcgtgg ctcgactacc acacctgtac gctcagagtg tccaacagaa
     1681 tgcacacggt tttcgatttc cacaagggca cccagttctt gcaacttagc aaccgttgcc
     1741 ttacgagctt caaagcggtc catgcctgca aattcaccag ccaactcgtt catagtaccg
     1801 tcgtcgttca tgacattgac ttgtggcaga ttgtggcgtt gtccaacgag gaagtcattt
     1861 ggatcgtgcg caggagtgat tttcacaacc cctgtaccaa attcaggatc cgcgtgctca
     1921 tccgcaacga ttgggattgg cttgttcaca attggaagga taacattttt accaatcaag
     1981 tccttgtaac gtggatcttc tgggttaact gcaaccgcaa cgtcaccgaa catggtttca
     2041 ggacgagttg tggcaacttg aagggcacga gaaccatctt ccaacatata attcatgtgg
     2101 tagaaagcac cctctacgtc cttgtggata acttcaatat cagaaagggc tgtgcgagcc
     2161 gctgggtccc agttgatgat aaattcacca cggtagatcc agcctttttt gtagagttct
     2221 acaaagacct tgcgaacggc ttttgacaag ccttcgtcca gcgtgaagcg gtcacgttgg
     2281 tagtccaaag acagacccaa cttgccccat tgttcacgaa tagtcgctgc atactcatcc
     2341 ttccattccc agactttatc gaggaatttt tcgcgcccca agtcataacg ggtcacgcct
     2401 tgctcccgca agcgttcctc aaccttagcc tgagtagcaa tccccgcgtg gtccatacct
     2461 ggaagccaga gggtatcaaa tccctgcatg cgtttttggc ggatgatgat atcttgaagg
     2521 gttgtatccc aagcgtgtcc caagtgtaac ttaccagtta cgtttggagg tggaatcacg
     2581 atagaataag gcttagcctc cgcatcgcct gacggcttaa aaacatcttg gtccaaccaa
     2641 gtttggtaac gaccagcctc aacctcggct ggattgtatt ttggtgataa ttcttttgac
     2701 attatttttt ctccttcagt attctttaaa aaaattggtt tatactatct tcaagctaga
     2761 aatagcccaa ttct
//
LOCUS       DBNFCK010000160         2728 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.160, whole genome shotgun sequence.
ACCESSION   DBNFCK010000160 DBNFCK010000000
VERSION     DBNFCK010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.160"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            13..1860
                     /gene="typA"
                     /locus_tag="AC1PIW_001830"
     CDS             13..1860
                     /gene="typA"
                     /locus_tag="AC1PIW_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002932699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HIP6606724.1"
                     /translation="MTKLREDIRNVAIIAHVDHGKTTLVDELLKQSQTLDERTQLDER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLTPIVVVNKIDKPSARPEEVVDEVLELFIELGADDDQ
                     LEFPVVYASAINGTSSLSDNPADQEETMAPIFDTIIEHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERKEIQEAKAG
                     DLIAVSGMEDIFVGETVTPTDAVEALPVLRIDEPTLQMTFLVNNSPFAGREGKWVTSR
                     KIEERLLAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IIKEIDGVKMEPFERVQIDTPEEYQGSVIQSLSERKGDMLDMVATGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPVVQGEIGGRHRGALVSIDQGKATTYSIMR
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITTAKQMTNVRSATKDQTSVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAERDKAAKKKKLATEE"
     gene            1878..2126
                     /locus_tag="AC1PIW_001831"
     CDS             1878..2126
                     /locus_tag="AC1PIW_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HIP6606725.1"
                     /translation="MFYLILAIMLVLFYIFIAPSNIKGTMNLIAAVFLLVALAIALVL
                     GFLRIMQFPKEIWVGLLMILIGFWAMRDIYQLNDDTKF"
BASE COUNT          802 a          540 c          580 g          806 t
ORIGIN      
        1 atagaggata ttatgacaaa acttagagaa gacatccgta acgttgccat tattgcccac
       61 gttgaccacg gaaaaacaac cctcgttgat gaattattga aacaatccca aacacttgat
      121 gaacgtacgc agttggatga gcgtgccatg gactcaaatg acattgaaaa agagcgtgga
      181 atcactatcc ttgctaaaaa cactgccgta gcttataacg gtactcgtat caacatcatg
      241 gacacaccag gacacgcgga cttcggtggt gaggttgagc gtatcatgaa aatggttgat
      301 ggtgttgtgc ttgtcgtgga tgcttacgaa ggaactatgc cacagactcg tttcgtattg
      361 aaaaaagccc ttgagcaaaa cttgactcca atcgtcgttg tcaacaagat tgacaagcca
      421 tctgctcgtc cagaagaagt agtggatgaa gtgcttgagc tcttcatcga attgggtgca
      481 gatgatgacc agttggaatt ccctgttgtt tacgcgtcag ctatcaacgg tacttcatct
      541 ttgtctgaca atccagcaga ccaagaagaa acaatggcac caattttcga taccatcatc
      601 gagcatatcc ctgcaccagt ggataactca gatgagcctt tgcagttcca agtatcgctt
      661 ttggactaca atgacttcgt aggtcgtatc ggtatcggtc gtgtcttccg tggtactgtt
      721 aaagttggtg accaagttac cctttctaaa ctagatggca caacgaaaaa tttccgcgtt
      781 acaaaactct ttggtttctt cggtcttgag cgtaaagaaa tccaagaagc aaaagctggt
      841 gatttgattg ctgtttctgg tatggaagac atcttcgttg gtgaaactgt gactccgaca
      901 gatgcagttg aggcacttcc agttcttcgt atcgatgagc caactcttca aatgactttc
      961 ttggtgaaca actcaccgtt tgcaggtcgt gaaggtaaat gggtgacatc tcgcaagatt
     1021 gaagagcgcc ttttggccga gttgcaaaca gacgtttctc tccgtgttga ccctactgac
     1081 tctccagata aatggactgt ttcaggtcgt ggtgaattgc acttgtctat cttgattgaa
     1141 accatgcgtc gtgaaggcta tgagcttcaa gtatcacgtc cagaagttat catcaaggaa
     1201 attgacggcg tgaaaatgga gccattcgag cgcgtgcaaa tcgatactcc agaagaatac
     1261 caaggttctg ttatccaatc cctatctgag cgtaagggtg atatgttgga tatggttgcg
     1321 acaggtaatg gtcaaactcg tcttgttttc cttgtgcctg cgcgtggttt gattggttat
     1381 tcaacagaat tcctttcaat gactcgtggt tacggtatca tgaaccatac tttcgaccaa
     1441 tacttgccag ttgttcaagg tgagatcggt ggtcgtcacc gtggtgccct tgtttctatc
     1501 gaccaaggta aggcaacgac atactctatc atgcgtattg aagagcgtgg taccatcttt
     1561 gtaaacccag gtactgaagt gtacgaagga atgattatcg gtgaaaactc gcgtgaaaat
     1621 gacttgacag ttaacatcac aactgcaaaa caaatgacca acgttcgttc agctaccaaa
     1681 gaccaaacgt ctgtaattaa gacaccacgt atcttgactc ttgaagaatc actagaattc
     1741 ttgaacgatg atgaatacat ggaagtaacg ccagaatcta tccgtcttcg taagcaaatc
     1801 ttgaacaagg cagagcgtga taaggctgcg aagaagaaaa aattagcgac tgaggaataa
     1861 tttctaggag aagaagcatg ttttatttaa tcctcgccat catgttagta cttttttata
     1921 tttttattgc acctagcaat attaagggga ctatgaattt gattgcggca gtatttttac
     1981 tggtagcatt agcgattgcc ttggtacttg gatttttaag aatcatgcaa tttccaaagg
     2041 aaatttgggt tggtctacta atgattctga ttggtttttg ggcgatgcgg gatatttatc
     2101 aattaaatga cgatacaaaa ttttagcaga gtctttgatt tttgaatacc gtaatacgat
     2161 attactaagg aattttatcc agctttttgg acagtagtgg caatctatta gctaaagaaa
     2221 ttatattctg tgatacatct tgaagaagct ctcgttattt ataataaatg acaagcgcat
     2281 tttcaaggtg tttttatgta gataaatttt aatactcaat gaaatcccca aaatagcaag
     2341 tttttagtac agtcatttaa attaactctg atacatcgcc gctttcagct tgccgcactc
     2401 tacgaaagtg actcgtttcg catgtcttat ttccaaccta gaacagcctc taggctgttc
     2461 tagctacctg cacatcggtt ccttgtctga aagctaattc attagaccat acaaggcaat
     2521 gagctgccgc cgccaacttt tatcaacatc ttagcaattt gcgttttaca gtctgaagct
     2581 gatctctata attgacggga gtgggaaaga catccataca ttaaagtaat taaagacaaa
     2641 ttaaataact atattagtca ttcaaaccta aatgagaggg aattgtttta taaaatattg
     2701 ctaacagttt tcccttagac aaactcat
//
LOCUS       DBNFCK010000161         2610 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.161, whole genome shotgun sequence.
ACCESSION   DBNFCK010000161 DBNFCK010000000
VERSION     DBNFCK010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.161"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(218..463)
                     /locus_tag="AC1PIW_001832"
     CDS             complement(218..463)
                     /locus_tag="AC1PIW_001832"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606726.1"
                     /translation="MKYFDTSKTTNQVVTCIRQLENTELEKELLQFIEECEKADSLSK
                     EYIERFFKIVDAFLEVFPGSSLDSQHLKKLLTLLHEK"
     gene            complement(478..2463)
                     /gene="uvrB"
                     /locus_tag="AC1PIW_001833"
     CDS             complement(478..2463)
                     /gene="uvrB"
                     /locus_tag="AC1PIW_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HIP6606727.1"
                     /translation="MINRNTENQFKLVSKYAPSGDQPQAIETLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVIARVNKPTLVIAHNKTLAGQLYSEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYS
                     DSVVSLRPGQEISRDQLLNALVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREIESLTGKVLGDVDHLAIFPATHFVTNDDHMETAIAKIQAELEEQL
                     KVFEAEGKLLEAQRLKQRTDYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PEDYLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYEMEQTETVVEQIIRPTGLLDPEVEVRPTMGQMDDLLGEINARVEKG
                     ERTFITTLTKKMAEDLTDYLKEMGVKVKYMHSDIKTLERTEIIRDLRLGVFDVLIGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADKVTESMR
                     KAMEETARRRQIQMAYNEEHGIIPQTIKKEIRDLISVTKAVTQDKEEVVDFNALNKDE
                     RKAMIKKLEGQMQEAAEVLDFELAAQIRDMVIELKNM"
BASE COUNT          678 a          661 c          516 g          755 t
ORIGIN      
        1 acgaaatata tcagtattag taaattgagt ctggttacag tatgactcac actttctcta
       61 ctttccaata gcgtcccaaa aatacttccc ctatcaacaa aatctctaat ttgatcataa
      121 gtgctaactc gaacaattaa ttccctaaat aatctagaat aagcactaat gttagaggta
      181 tctctataaa agatttccgc ccacacctct tctgttttta tttttcatgg agcaaagtca
      241 agagtttctt taagtgctgc gaatccaagg atgatcctgg aaacacctca agaaaagcat
      301 cgacaatttt aaaaaaacgt tctatatact ctttggatag agagtctgct ttctcacact
      361 cttcaataaa ctgtaacaac tctttttcta gctctgtatt ttccaattgt ctaatacagg
      421 ttaccacttg attagttgtt ttcgacgtat caaaatattt cataactacc tccatatcta
      481 catattcttc aactcaatga ccatatcgcg aatctgggct gccagttcaa agtcaagcac
      541 ttctgcggct tcctgcatct gaccttccag tttcttgatc atggccttgc gttcatcttt
      601 attaagagca ttgaagtcca caacctcttc cttgtcctga gtgacagcct tggtcacgct
      661 aatcaggtca cggatctctt tcttaatggt ctgtggaata atgccatgct cttcattata
      721 agccatctgg atttgacggc ggcgggctgt ttcttccatg gccttccgca tggactcggt
      781 gaccttgtcc gcatacataa tcacatgacc ttcagagtta cgagccgctc gaccgattgt
      841 ctggatgagc ccccgttcat tacggaggaa gccctccttg tctgcgtcta agatagcgac
      901 tagactaact tctggcacgt caatcccttc acgaagcagg ttaatcccta tcaaaacatc
      961 aaagacaccc aagcgaagat cacggataat ctccgtacgc tccaaggtct tgatgtccga
     1021 gtgcatatac ttgaccttga cgcccatttc tttcaggtag tcggtcaagt cctctgccat
     1081 tttcttggtc agggtagtga taaaggttcg ctcgcctttt tcaacacggg cattgatttc
     1141 acctaggagg tcgtccattt ggcccatagt tggacggact tccacttctg gatccaaaag
     1201 cccggtcggc cggataatct gctcgacaac ggtctctgtc tgctccattt catagtcacc
     1261 cggcgtcgca gatacataga caatctggtg gacatgactc tcaaattctt ccctgcgcag
     1321 cggacgatta tccaatgcac tcggtagacg gaaaccatag ttaaccagca tctccttgcg
     1381 tgagcggtca ccattgtaca tccccttaat ctgccccatg gtcatgtggc tctcgtcaat
     1441 cataatcaga tagtcctcag ggaaaaagtc cagcagggta taaggaggct ccccctcact
     1501 tcgcccgtcc atgtgtcgtg aatagttctc aactccgttg gtatagccca tctcccgaag
     1561 catctcgata tcgtagtcgg ttcgttgttt caatcgctga gcttctaaga gttttccttc
     1621 tgcctcaaag accttgagtt gctcttctag ttccgcctga atcttggcaa tggccgtttc
     1681 catgtggtca tcgttggtca cgaagtgggt ggcagggaaa atggccaagt ggtccacatc
     1741 gcccaaaacc ttgcctgtca ggctttcaat ctcacgaatg cggtcgattt catccccgaa
     1801 aaactccaca cgaaaggcat gttcgtcacg ggaagctggg aaaatctcca ccacatcgcc
     1861 acgcacacgg aaccgtcccc gttggaagtc gatgtcgttc cgctcaaact ggatatccac
     1921 caaggcattg agcaactgat cacgggaaat ctcctgacct ggtcgcaggc tgaccacgct
     1981 atctgaatat tccttgggtg aacccaaacc gtagatacaa gaaacagaag ctaccacaat
     2041 gacatcattg cgttctagca gagctgaggt cgcagagtga cggagtttgt caatctcatc
     2101 attgaccgaa ctatcctttt cgatataggt atcgctagac ggtacataag cttctggctg
     2161 gtagtaatca tagtaagaca cgaagtattc gaccgcattt tctggaaaga attccttgaa
     2221 ctcactataa agctgaccag ccaaggtctt gttgtgggcg atgaccaagg ttggcttatt
     2281 gacacgggcg ataacctggc tcatggtata ggtcttacca gtaccagtcg cccccatgag
     2341 aatctgggct ttttcgcccc cctcgatgtt atcaaccaag gtttcaatgg cttggggctg
     2401 gtcaccagac ggtgcatatt ttgacacaag tttaaattgg ttttcagtat ttcgattgat
     2461 catactccta ttttatcata agttcgacta atggagaaac attctaaacc attcacaact
     2521 atgtccaaaa tattaaattt tctcacattt taatgacttt gtatgtcaga aattctgaca
     2581 ttataaaaac ctcaattttg cattaatttt
//
LOCUS       DBNFCK010000162         2592 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.162, whole genome shotgun sequence.
ACCESSION   DBNFCK010000162 DBNFCK010000000
VERSION     DBNFCK010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.162"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..645
                     /locus_tag="AC1PIW_001834"
     CDS             <1..645
                     /locus_tag="AC1PIW_001834"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HIP6606728.1"
                     /translation="TTTHLSNPSAQRSYTVYIDPGHGGRDSGASYGGVHEKNLALSVS
                     NKLRENLLQYGINVLMTRTGDYDVDFKTERSRMTNASNADLFISIHFNATGAGVSNAT
                     GIETYWYQYNPEYQPKINKEMHNNPTRLAESEILANKVQESLIKETGAVNRGVRRETF
                     AVLRETAIPAILVELGFMDNPSELQVIKQDSYHTRLAKALAQGVMNWYGAVEGK"
     gene            723..1151
                     /locus_tag="AC1PIW_001835"
     CDS             723..1151
                     /locus_tag="AC1PIW_001835"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013954.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HIP6606729.1"
                     /translation="MEQSSKANSILSSVMLSQAILESAWGTSYLATHGNNLFGIKADA
                     AWTGATIEIITNEYRDGEKKQEKHLFRKYNSWNESVADYAKFFTSTPWRIKNYQSFRE
                     ATDYQQAILALRQSGYATDPKYGEKLRSIIENYKLYLLDN"
     gene            complement(1211..>2592)
                     /locus_tag="AC1PIW_001836"
     CDS             complement(1211..>2592)
                     /locus_tag="AC1PIW_001836"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HIP6606730.1"
                     /translation="TFYSSYDFKWPKRFSRILLGYITLITIVPMINKTVVSNANISLN
                     YWRMYVTYGQFGLPLSLASFYEDSKITKPEGYSVPKLNEVLEKYPPETEKQTIRPNII
                     FIQNESQSDFSNLQGLNMEPNPLSNQHALTDNTIHGTLNVSVFGGGTANTEYEVLTSN
                     PISLLSSNLFPYQQIITQDRPSFATYLKNKNYDTVALHPQSGNNYNRNAVYPLLGFNK
                     SYFLDSEPAISSLAPLTIDRGWPSDQFLFNGIKELYTQKGDQPLFSFVVTMQGHGGYP
                     STEEIYPREVSINGSTSEYLAETEFLTSMKRTDEAFADLITFFSTYKEPTVIVMYGDH
                     QPSLTQEFYAQFMDENNPAAKYSTPLVIWSNFDIRERESTTISPNYLVPYLMDILSES
                     DYALPRSPYQQFLSDMQIEAPIITSWGNIDNNGQQIEDLSTLPLYQTYLQLEYNSAID
                     KQPLTDLYE"
BASE COUNT          807 a          430 c          559 g          796 t
ORIGIN      
        1 acaacgactc atttatcaaa tccgtcggca cagcgatcct acacagtcta tattgaccca
       61 gggcacggag ggcgagactc gggtgcttct tacggtggcg tacatgaaaa aaatcttgca
      121 ttgtctgttt caaataagtt gcgtgaaaat ctcttgcaat atggaatcaa tgttttgatg
      181 acccgtacag gtgattatga tgtagacttc aaaacagaga ggtcacgaat gacgaatgca
      241 agcaacgcag acttatttat tagtatccat tttaatgcga cgggtgcagg agtttcgaat
      301 gcgactggta tcgaaacata ttggtatcaa tataatccag aataccaacc taaaattaat
      361 aaagaaatgc ataataaccc tactcgttta gctgaaagtg agattttagc caacaaggtc
      421 caggagagtt taattaaaga aacaggtgca gttaaccgtg gtgtaagaag agaaactttt
      481 gcagtccttc gcgagacagc aattcctgcc attctagtgg agctaggatt tatggataat
      541 ccttcggagt tgcaagttat caaacaagat tcttaccaca ctcgccttgc gaaagcattg
      601 gcacaaggtg taatgaattg gtatggtgct gttgagggaa agtagaaatt gcaggtacag
      661 gtattgaaac aggaaaactt tctaatgatc aaaaaagttt catagccaat ctagtacctg
      721 caattgaaca aagttcaaaa gctaattcta tcctttcgag cgtcatgtta tctcaagcca
      781 tcctcgaaag tgcatgggga acgtcatact tggcgactca tgggaataat ctttttggca
      841 tcaaagcgga tgctgcgtgg acaggtgcta caattgaaat cattaccaat gaataccgtg
      901 atggtgagaa gaagcaagaa aagcacttgt ttagaaaata taattcatgg aatgaaagtg
      961 tagcagacta tgctaagttc tttacctcta ctccgtggag aataaaaaat taccagagtt
     1021 tcagagaggc aactgattat caacaagcga ttcttgcgct aagacaatca ggatatgcta
     1081 ctgatccgaa atatggtgaa aaattacgat caattatcga gaattacaag ctttatcttt
     1141 tagataatta agttttttgg gtgattgatg ataaaatagc cttgctcaaa accgagcaag
     1201 gctattttta tcactcatat aaatctgtta aaggttgttt gtcaatggca ctattgtatt
     1261 ctagttgtaa gtaggtttgg taaagtggta gggttgatag gtcttcaatt tgttggccat
     1321 tattatcgat atttccccag ctagtaatga taggtgcttc gatttgcata tcactcaaaa
     1381 attgttggta aggtgatcga ggaagggcat agtcggattc ggataaaata tccatcaaat
     1441 atggaacgag atagtttggg ctgattgttg tagattcgcg ctccctaata tcaaaattcg
     1501 accaaatcac taaaggggta gaatatttag cggcaggatt attttcatcc ataaattgtg
     1561 cgtaaaattc ctgagtcaaa ctaggttggt gatcgccgta catgacgatg actgttggct
     1621 ctttgtaagt actaaagaaa gtaattaaat ctgcaaatgc ttcatctgtt cttttcatgc
     1681 tggtcaaaaa ttctgtttca gctaagtatt cagatgttga accattaatg ctgacttcgc
     1741 gaggataaat ttcttccgta cttggatagc ctccatgtcc ttgcatggta acaacaaagc
     1801 taaatagagg ctggtctcct ttttgggtat aaagttcttt tattccgttg aacaagaatt
     1861 gatcagatgg ccagccacga tcgattgtta gtggtgctaa gctactaata gcaggttccg
     1921 aatctaaaaa ataagattta ttaaacccca gtagaggata cactgcattg cggttgtaat
     1981 tatttccgga ttgaggatgt agagctacag tgtcgtaatt tttatttttt agataagttg
     2041 cgaaacttgg acggtcttgt gtgataatct gctgatatgg gaataaatta gaagataata
     2101 aactgattgg gttacttgtc agcacttcat actcggtatt tgcagtacca ccgccaaaga
     2161 ctgagacatt gagggtgcca tgaatagtat tgtctgttaa agcatgttgg tttgatagcg
     2221 gattaggctc catatttaag ccctgtaagt ttgaaaaatc actctgtgat tcattctgta
     2281 taaagataat attaggtctg attgtttgtt tttctgtttc aggagggtac ttctcaagaa
     2341 cttcattaag tttggggact gagtatcctt caggcttagt tattttacta tcttcataga
     2401 aagaggcgag tgagagtggt agcccaaatt gaccataggt gacgtacatt ctccagtagt
     2461 ttaatgatat atttgcatta gagacgacag ttttattaat cattggaacg attgttatta
     2521 aagttatata gcccagtaaa attctgctga aacgtttcgg ccatttgaaa tcataactag
     2581 aataaaacgt tc
//
LOCUS       DBNFCK010000163         2538 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.164, whole genome shotgun sequence.
ACCESSION   DBNFCK010000163 DBNFCK010000000
VERSION     DBNFCK010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.164"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            49..885
                     /locus_tag="AC1PIW_001837"
     CDS             49..885
                     /locus_tag="AC1PIW_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HIP6606731.1"
                     /translation="MMNIIEVKNLKYKYNQEDEQYTLNDVSFHVKQGEWLSIIGHNGS
                     GKSTTVRLIDGLLEAESGDIYIDGDALTIDNVWDKRRLIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGIPLEEMRSRVDEALELVGMTDFKTREPARLSGGQKQRVAIAGVVALRPK
                     IIILDEATSMLDPEGRLDLIKIVREIKERHGMTVISITHDLDEVALSDRVIVMKNGQV
                     ESISTPNELFMREDLVDLDLDRPFTTELASSLRQTGLDLPLRYFTEEELEETLWELIS
                     KK"
     gene            861..1703
                     /locus_tag="AC1PIW_001838"
     CDS             861..1703
                     /locus_tag="AC1PIW_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002267082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HIP6606732.1"
                     /translation="MGIDLQEVSYTYQAGTPFEGRALFGVDLEIVDGSYTALIGHTGS
                     GKSTILQLLNGLNVPTEGRVVIDDVVITATSENKDIKQVRKKVGLVFQFPESQVFDET
                     VLKDVAFGPQNFGVSQEEAETIAREKLALVGIDESLFERSPFELSGGQMRRVAIAGIL
                     AMEPSILVLDEPTAGLDPAGRQELMDIFKKLHESGMTIVLVTHLMDDVANYADFVYIM
                     EKGKLVRSGKPVDIFQEVDFLESIQLGVPKITKFAANLERRGFVFERLPITIEEFTGM
                     VTHG"
     gene            1696..2490
                     /locus_tag="AC1PIW_001839"
     CDS             1696..2490
                     /locus_tag="AC1PIW_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T family protein"
                     /protein_id="HIP6606733.1"
                     /translation="MDKLILGRYIPGDSIIHRLDPRSKLLAMFGFLLMIFWANNLVTN
                     ALLIAFVLGMVVLSRIRLSFFINGLKPMIGIILFTTFFQVFFTPGATILWEFWIFKLS
                     VEGLQQAAIIFVRFVLIIFFSTLLTLTTTPLSLADGIESGLAPLKKFKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQRARGVDFNEGNLIQKVKSVIPILIPLFASSFKRADAL
                     ATAMEARGYQGGDGRTKYRILEWKLADTLAILVMLALAGLLFILKK"
BASE COUNT          692 a          374 c          629 g          843 t
ORIGIN      
        1 ctatgattat ttcaagggtg cgtccttctt gtttaaagat acatttaaat gatgaatatt
       61 attgaagtaa aaaatttaaa atataaatac aaccaagagg atgagcaata tactctaaat
      121 gatgtttcgt ttcacgtgaa acagggagag tggctatcta ttattggtca taatggttcg
      181 ggtaaatcaa caacagttcg tttgattgat ggtttgttgg aagctgaatc aggtgatatt
      241 tatattgacg gtgatgcctt aaccattgat aatgtatggg ataagcgacg cttgattggt
      301 atggtttttc aaaatccaga taaccaattt gtaggcgcta cagttgaaga tgatgttgca
      361 tttggtttag agaatcaggg aatccctttg gaggagatgc gtagtcgagt tgacgaagct
      421 ttggaattag ttggaatgac tgatttcaaa acacgtgagc ctgctcgttt atctggtggt
      481 cagaagcaga gggtggcgat tgctggagtg gttgccttgc gtccaaaaat tattattttg
      541 gatgaggcta cgtctatgtt ggatccagaa ggtcgtctcg acttgattaa gattgtccgt
      601 gaaattaagg agcgtcatgg tatgacggtt atctctataa cacatgattt ggatgaggtt
      661 gcattgagtg atcgagttat cgtgatgaaa aatggacagg ttgaatcaat tagcactcca
      721 aatgaactat ttatgcgaga agatttggta gatttagact tggatagacc gtttacgaca
      781 gaactggctt catctttgcg ccagactgga cttgatttac cgctccgcta ttttacagag
      841 gaagaattag aggaaacttt atgggaattg atctccaaga agtaagctat acctatcaag
      901 ctggcactcc ttttgagggg cgtgcgcttt ttggtgtgga tttggagatt gttgatggct
      961 cttacacggc ccttattggg catacgggtt cgggcaaatc aacgatttta caactcttga
     1021 atggattaaa tgtaccaaca gaaggaagag tagttattga tgatgttgtg attacagcga
     1081 cgtctgaaaa taaggatatt aagcaagtca ggaagaaagt ggggttggtg ttccaatttc
     1141 ctgaaagtca agtgtttgat gaaacggtct taaaagacgt tgcttttggt ccacaaaatt
     1201 tcggagtttc tcaggaagag gctgaaacga ttgcgcgtga aaagttggcc ttggttggaa
     1261 ttgatgaaag cttgtttgag cgtagtcctt ttgaactatc tggtggtcaa atgcgtcggg
     1321 tagctattgc tgggattctg gcaatggaac caagtatctt agttttggat gaaccaacgg
     1381 ctggattgga tcctgcgggt cgtcaggaat tgatggatat tttcaaaaag cttcatgaat
     1441 caggcatgac aatcgttttg gtaactcatt tgatggacga tgtggctaac tatgctgatt
     1501 ttgtttacat tatggaaaag ggaaagctgg tccgttctgg taaaccagtc gatatttttc
     1561 aagaggtaga ttttttagag agtattcagt tgggagtgcc taagattact aagtttgcag
     1621 ctaatttgga gagaagaggc tttgtatttg aacgtcttcc tattacaatt gaagaattta
     1681 cggggatggt gacacatgga taaattaatt ttaggtcgct atattcctgg ggattcaatt
     1741 attcatcgct tggatccaag aagtaagctt ttagcaatgt ttggatttct attgatgatt
     1801 ttttgggcca ataatttggt caccaatgcc ttgttgatag catttgtttt aggaatggtt
     1861 gttttatcac gtattcgctt atcatttttt attaatggtt tgaaaccgat gataggaatt
     1921 attctattta caactttctt tcaggttttc tttacacctg gagcgaccat tctatgggag
     1981 ttttggattt ttaaattatc tgtagaaggg ttgcaacagg cggcgattat ttttgttcgc
     2041 tttgttttga ttattttctt ctcaacccta ttgaccttga cgacgacacc ccttagcttg
     2101 gctgatggta ttgagtcggg gcttgcacct ttgaagaaat ttaaagttcc tgtgcatgaa
     2161 attggtttga tgttgtccat gagcttgcgt tttgtaccga ccttgatgga tgataccact
     2221 cggattatga atgctcagag agctcgtgga gtggacttta atgagggaaa tcttattcag
     2281 aaggtaaagt cagttattcc tattttgatt ccgttattcg catcaagttt taagagggca
     2341 gatgctcttg caacggcgat ggaggcgcgt gggtatcaag gcggcgatgg gagaaccaag
     2401 taccggattt tggaatggaa gttagcagat acgttagcaa ttttagttat gttagcttta
     2461 gccggactct tatttatatt gaaaaaataa gtaaaaaacg ctagaaatag cgtttttatt
     2521 gtctaatctc aatttgtc
//
LOCUS       DBNFCK010000164         2488 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.165, whole genome shotgun sequence.
ACCESSION   DBNFCK010000164 DBNFCK010000000
VERSION     DBNFCK010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.165"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            35..1060
                     /gene="adhP"
                     /locus_tag="AC1PIW_001840"
     CDS             35..1060
                     /gene="adhP"
                     /locus_tag="AC1PIW_001840"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HIP6606734.1"
                     /translation="MKAVVVNPESTGVVVVEKELRPLEAGEALVQIEYCGVCHTDLHV
                     ANGDFGKVPGRVLGHEGIGIVTEIAPGVTSLKVGDRVSVAWFFQGCGMCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPEGLDPAQASSITCAGVTCYKAIKEAHL
                     EPGQWIAIYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLELAKEVGADVTINGLEVE
                     DVPGYIKEITGGGVHSTVVTAVSKVAFNQAIDSVRAGGYVVAVGLPSEYMDLSIVKTV
                     LDGIKVVGSLVGTRKDLEEAFHFGAMGLVVPVVQKRPVEDAEAVFNEMVAGTIQGRMV
                     LDFCHSH"
     gene            complement(1141..2424)
                     /gene="hisS"
                     /locus_tag="AC1PIW_001841"
     CDS             complement(1141..2424)
                     /gene="hisS"
                     /locus_tag="AC1PIW_001841"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HIP6606735.1"
                     /translation="MKLQKPKGTQDLLPQDSAKWQYVENFTRSIFKQYNYAEIRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGERHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PAKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSNNPATDVETIAMAYHFFEELGIKD
                     IRLHLNSLGNPESRAAYRQALIDYLTPLKEQLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDYLDEESTVHFEAVRSMLDNLGITYTIDTNMVRGLDYYNHTIFEFMTEVG
                     GNDLTICAGGRYDGLVTYFGGPETPAFGFGMGIERLILVLEKQGIELPLDTQLDVYIA
                     VLGQEANGGALDLVQAIRKQGFRAERDYLDRKLKAQFKSADVFGAKAIITLGGSEIES
                     GQVVVKNNQTRSQVETSLEALKTDFASILEELEKQ"
BASE COUNT          635 a          564 c          580 g          709 t
ORIGIN      
        1 atgtacataa aatattaaaa caggaggtct gcttatgaaa gcagtcgttg taaatccaga
       61 atctactggt gttgtcgttg tcgaaaagga acttcgtcca ctcgaagctg gtgaggcttt
      121 ggtccaaatt gagtactgtg gtgtttgtca cactgacctc cacgttgcca atggtgattt
      181 tggtaaggtt cctggtcgcg ttcttggtca cgaaggaatc ggtattgtta cagaaatcgc
      241 acctggtgtt accagtctaa aagttggtga ccgtgtcagc gttgcttggt tcttccaagg
      301 ctgtggtatg tgtgaatatt gtacaaccgg tcgcgaaact ctctgccgta ccgtaaaaaa
      361 tgcaggctac tctgttgacg gtggtatggc tgagcaatgt attgtaacag cggactatgc
      421 agtaaaagtt ccagaaggtc ttgatccagc tcaagcaagt tctatcactt gtgctggcgt
      481 tacttgctac aaagcgatta aagaagctca cttggaacca ggtcaatgga ttgctatcta
      541 tggtgctggt ggacttggaa accttgctgt acaatacgct aaaaaagtct tcaacgctca
      601 tgttatcgcc gtagacatca ataatgataa attggaactc gccaaagaag ttggtgcaga
      661 tgtgacgatc aacggacttg aagtagaaga tgttcctgga tatattaaag aaattactgg
      721 tggcggtgta cactctactg ttgtaacagc tgtttccaaa gtagccttca accaagctat
      781 tgacagtgtt cgcgctggtg gatatgtagt agctgtcgga cttccatctg aatacatgga
      841 cctcagtatc gtgaagactg tgctagacgg tatcaaagtg gtcggctcac tagttggtac
      901 tcgtaaagat ttagaagagg ccttccactt cggtgctatg ggactagttg ttccagtcgt
      961 tcaaaaacgc cctgtcgaag atgccgaagc agtcttcaat gaaatggttg ccggtactat
     1021 ccaaggtcgt atggtcttag acttctgcca ctcacactaa tgcgttctcc actattttca
     1081 agaagtgatt atccaatcgc ttcttttttg ctttctaaaa taaaaagaag cagctttaga
     1141 ctactgcttc tccaattctt ctaaaatact tgcaaaatct gttttaagtg cttccaagct
     1201 tgtttcaact tgacttcttg tctggttatt tttgaccaca acctgtccag attcaatttc
     1261 gctaccaccc agggtaatga tagccttggc tccaaagaca tctgctgact taaactgggc
     1321 cttgagcttg cggtccagat agtcacgttc tgcacggaaa ccttgcttgc gaatggcttg
     1381 aacaagatca agcgctccgc cattggcctc ctgacccaaa accgcgatgt aaacatctaa
     1441 ctgggtatcg agagggagct cgatgccttg cttttcaagg actaggatga ggcgctcaat
     1501 gcccatacca aagccaaaag ctggcgtttc tggtccacca aagtaggtca ccaaaccgtc
     1561 gtaacgtcca ccggcacaga tggtcaggtc attgccaccc acttctgtca taaactcgaa
     1621 aatagtatgg ttatagtagt ccaaaccgcg taccatgttg gtgtcaattg tgtaagtaat
     1681 gcctaggttg tccagcatgg acctgactgc ctcgaagtgg actgtacttt cttcatctag
     1741 atagtccaag atggacggtg cattttccac agcaaccttg tcttcttttt ccttggaatc
     1801 caacacacgc aaaggatttt cttctagacg gcgctggcta tctttagaca actgttcctt
     1861 gagcggcgtc agatagtcaa tcaaagcctg acggtaggca gcacggctct ctggattgcc
     1921 cagactgttg aggtggaggc gaatgtcctt gatccccaac tcttcaaaga agtgataggc
     1981 catagcaatg gtttctacgt ctgtcgctgg gttattggaa ccaaagcact ccacaccaat
     2041 ctggtggaac tgacggagac gacctgcctg cggacgctca tagcggaaca ttgggcccat
     2101 gtaatagaac ttagcaggtt tttgtacttc tggagcaaac agcttgttct ctacatagga
     2161 gcgaacaacc ggtgccgtcc cttctggacg gagggtgata tgacgctctc ccttgtcata
     2221 gaaatcgtac atctccttgg tcacaatgtc cgtcgtatcg ccaaccgaac ggctgatcac
     2281 ctcgtaatgc tcaaagattg gtgtgcgaat ctcagcgtaa ttgtactgtt tgaaaatgct
     2341 gcgggtaaag ttttccacgt actgccattt ggcagaatcc tgcggtagta aatcctgtgt
     2401 tccctttggt ttttgtagtt tcataataaa gtgtatagag ccaacttata tcgctcgaag
     2461 ttgtgcttct tcctttccaa attaagtc
//
LOCUS       DBNFCK010000165         2470 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.166, whole genome shotgun sequence.
ACCESSION   DBNFCK010000165 DBNFCK010000000
VERSION     DBNFCK010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.166"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            75..1772
                     /locus_tag="AC1PIW_001842"
     CDS             75..1772
                     /locus_tag="AC1PIW_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIP6606736.1"
                     /translation="MDEQNQFNWVNFYKEFAWKLVDYKDNRAELVEKVKAIYTKTKIH
                     MPTLEKDNRLIDIDPFTIFGLFNKQIKEENRIKILTAIAELFNLQTEIPSSFESIPVL
                     MNQNATFYYFIGDRDENDIDDLWELFIAALTYSKEPTAEKKEKFSHYFDLAINKKGNG
                     NSKITMALYWIAPDFFLSLDSQNEAYVYESGEIPTDIVQKLPKMKSKISADDYFYISD
                     SMHHYLNSAESKLKDFKELSSMAWKYSKQINQEEKERSAGTISPLADETDTGNEIGSE
                     EVVEDSYTKNHFLSEVYMTEEEYDQLTSILQMKKNIILQGAPGVGKTFIAERLAFSLM
                     GKPDLERVMTIQFHQSYSYEDFIMGFRPSTSGFELRRGAFYTFCKKAESDKSHNYFFI
                     IDEINRGNLSKIFGELFMLIENDKRGKSLQLLYSDEQFSIPENLYIIGMMNTADRSLA
                     LLDYALRRRFAFFDIKPGFSTTGFKDYQANLKNEKFDRLISCVEKLNQAISTEEVLGD
                     SFCIGHSYFCGLSAEVLEQGLLEAIVEYELIPLLKEYWFDEPTKIYDWSSKLRSAIQ"
     gene            1769..>2470
                     /locus_tag="AC1PIW_001843"
     CDS             1769..>2470
                     /locus_tag="AC1PIW_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methylcytosine restriction system specificity
                     protein McrC"
                     /protein_id="HIP6606737.1"
                     /translation="MIPVQNIYYMLSYAFKILHEQEYKRIATEEFKNAADLLAEIMII
                     SLSIQVKRGLGREYRSQTESLSALKGKINISESLTPPNWRRKQLVCQYDDFSLDSRMN
                     RIIKASIEILLKADISRDRKKKLRKLLVFFGEVSKINLHSINWNLQYNRNNQSYQLLM
                     SICYLVVNGLIHTEREGNKKLMNFLDERRESLLYEKFILGYYKKHYPQIQVTASQIPW
                     ALDDGFGEMLPIMQSD"
BASE COUNT          863 a          410 c          500 g          697 t
ORIGIN      
        1 gtttaatgat aaaatatagg taaattcgaa ctcataattt aataggtgta gatacaacac
       61 cgttggagga tactatggat gaacaaaatc aattcaattg ggtaaacttt tataaggaat
      121 ttgcttggaa attagtagac tataaagata atagagcaga gctggttgag aaagtaaaag
      181 ctatctatac caaaacaaag atacatatgc ccacgttaga aaaggacaat cgactgatag
      241 atattgatcc ttttacgata tttggactct tcaataagca aataaaagag gagaatcgca
      301 ttaaaattct gacagctata gcagaattat tcaacctcca aacggaaatc ccatcctctt
      361 ttgaaagtat accagtactg atgaatcaga atgcgacttt ctattatttt atcggagatc
      421 gtgatgagaa tgatattgat gacttatggg aacttttcat tgctgctttg acctatagca
      481 aggagcctac ggctgagaag aaagaaaaat tttcacacta ttttgatttg gctataaaca
      541 agaaaggaaa tggcaacagc aagattacga tggccttgta ttggattgct cccgactttt
      601 tcttgagctt agatagtcaa aatgaagcgt atgtgtatga gtctggggag attccaactg
      661 acattgtcca aaaattacca aagatgaagt caaaaatttc tgccgatgat tatttttata
      721 tctcggactc tatgcatcat tatttaaaca gtgcagagag caaactgaaa gattttaaag
      781 aattatcatc catggcttgg aaatactcta aacagatcaa tcaagaagag aaggaaagaa
      841 gtgctggaac aatttctcca ctggctgatg aaactgatac tggaaacgag ataggtagtg
      901 aagaggtagt agaagatagc tatacgaaaa atcacttcct atcagaagtc tatatgacag
      961 aagaagagta tgatcaatta acctccatac tccaaatgaa aaagaatatc atcttacaag
     1021 gagcgccagg agttggaaag acgtttattg cagaacgcct agcattttca ttaatgggta
     1081 aaccagattt agaaagggtc atgacaattc agtttcacca aagctattcc tacgaggact
     1141 tcatcatggg ctttaggcct tcgacaagtg gctttgagct gagaagaggg gcattctata
     1201 ctttctgcaa aaaagcagag tctgacaaga gtcataatta tttctttatt attgatgaaa
     1261 taaacagggg aaatttgagc aaaatttttg gtgaattgtt tatgctgata gaaaatgaca
     1321 aacgaggcaa gtctttacag ctcctctact cggatgagca attctcaatt ccagaaaatc
     1381 tatatatcat cggtatgatg aataccgcag ataggagttt ggccctactt gattatgcac
     1441 tgagacgacg ctttgccttc tttgacatca agccaggttt ttcaactacg ggattcaaag
     1501 attatcaagc aaatctgaaa aacgaaaaat tcgatagact catttcctgt gtagaaaagc
     1561 taaatcaggc catttcaaca gaggaagtac tgggagatag cttctgtatt gggcatagtt
     1621 acttctgcgg tttatcggca gaagtactgg aacaaggttt gctagaagcc attgttgaat
     1681 atgaactgat tcccctcttg aaagaatatt ggtttgatga accgacaaaa atttatgatt
     1741 ggagtagtaa gttaaggagt gccatccagt gatacctgtc caaaatatct actatatgct
     1801 atcctacgca ttcaaaatcc ttcatgagca ggaatataaa cggatcgcta ctgaagaatt
     1861 taaaaatgct gcagatctac tggcggaaat aatgataatc agcctctcga tacaggtgaa
     1921 aagaggactt ggtcgagagt atcgttctca aaccgagtca ctctcggcac ttaaaggtaa
     1981 aataaatatc tctgaatctt taacacctcc gaattggcgt agaaagcaac ttgtttgcca
     2041 atacgatgat ttttctcttg acagtagaat gaatcgcatt atcaaagcaa gcattgagat
     2101 actgctaaaa gctgatataa gcagggacag gaaaaagaaa ttgagaaaac tcttagtttt
     2161 ctttggagaa gtctcaaaaa ttaatctaca ctcaatcaat tggaatttac aatacaatcg
     2221 aaacaatcaa tcgtatcaat tactaatgtc aatctgctat ttggtggtga atggattaat
     2281 tcatacagag agggagggga ataagaaatt gatgaatttt cttgatgagc ggagggaatc
     2341 cttgctatat gagaagttca ttcttggata ttataaaaag cattatcccc aaatacaggt
     2401 taccgcctct cagatacctt gggcactaga tgatggtttt ggagaaatgc tccccatcat
     2461 gcagagtgat
//
LOCUS       DBNFCK010000166         2465 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.167, whole genome shotgun sequence.
ACCESSION   DBNFCK010000166 DBNFCK010000000
VERSION     DBNFCK010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.167"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            154..2332
                     /gene="yzpA"
                     /locus_tag="AC1PIW_001844"
                     /pseudo
     CDS             154..2332
                     /gene="yzpA"
                     /locus_tag="AC1PIW_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027584.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing zinc-binding protein
                     YzpA"
BASE COUNT          867 a          373 c          535 g          690 t
ORIGIN      
        1 aagtttttgc gaaatgtagc atgctattta ttacagacaa ctgtgaaaca gataaaaaac
       61 taatgaaaaa caatgttcta tgagatgttt tttttataat aaaactagga caatatgtgt
      121 cgttaattgt taatacagat ataaggagaa aagatgaata gcaagatttt ttcgttgaga
      181 aaaagtaaga tgggtttagt ttcggtggca attgcttttc tatggattgg tacagggatg
      241 aatatggaaa ccgcaatggc tgaggagact gacgctacag ctcttgaaac tcaattagaa
      301 agcacggaat catcgcttac caatacagtt tcggaaaatg ctgaagctga ggaagtgact
      361 gatgaggtac cgagtgaaga aaaaaaaatc ggaagagatg gaggatatgg aggaagaact
      421 ttcatttatt gaaaaccatc tagaggttgc tccgattcaa gctggagatc aaaccatctc
      481 agggaatacc actcctggtg gatatgtagc gattacgata gatggggaag caattacttc
      541 tattgagaat attttggaag cagatgataa gggagatttt tcttatcggt tgagtaaacc
      601 acttgctcac agccaaactg tggaaattag tgctttgccg aaacagtttt ggactctaga
      661 ggcagattct gaagagcgta aggtagtagt gaggactaat cgacatccag aggcttatga
      721 aattccagca aaaagattag aaaaaacatc taatggaatg catcaagtgt tcatcgaacc
      781 agtttttgaa catacgagca aagttattgg tcatacctct gtcaaaggtt cagtctatct
      841 atcaataaat ggtagttttg tctctgataa gacactaatt gatcctaaag atggacggtt
      901 tgaggttact ttttccgaga gtttagcagg ttcaaaattt aaagcagatg atagattggt
      961 tttgtctttt gtatcagaag atggtcagcc ggtgattact aatactattg taaaaccatt
     1021 ggtaaaagaa aaagtatctt cacaaatgac agtaaaacct ttatcaagtg cgacttcagt
     1081 tttggaagga actacatttc ctttaggtcg tgtgcatcta tacaatgctg atacttcaga
     1141 gtttatcatg gaagctattg ctgatgagac aggtcactat aagattgctt tgcctgccct
     1201 acaatccgag gataagtact acagattgac tcacaatcaa caagaggact tggtatctgt
     1261 ccatcttgat acagttgatg gttcaagtat attattggat aaatctgtta tggcatcttt
     1321 agcaacttat ttacaagatg cagatatgga tgaagcgaca gatgaagatc ctatcattgt
     1381 acctaaactg cataataaaa aagattatat tgtcggtcga acgatacatc ttaatgccta
     1441 cgttcgcatg gtttcgtcaa tcaaaggaaa acagtatcct ccagttcaag tggacgaatt
     1501 aggatttttt ggtttccaaa ttcaagattt acaattgcct tttgaaaaag gtgagagaat
     1561 tcgctttgaa atcattgacc cggttacaaa taatatcatc gctagtaagg aagaagttgt
     1621 agggcaatat ttggaagatg aagacgtgat ggatttacca tttcaagtag aaaaagtcac
     1681 tacagatcat ggttacatca gtggcaaaac agctccagat gtaatgattg aactagtatc
     1741 aactcagaat ggcgaggaga taattggtaa aacttcaact gattcaacag gtcgattcga
     1801 atttgatttg ggtagtcgtg ttttaaaaaa tggagaaacc ttatcgttta gagcgtttga
     1861 taaagaaggt gaacaggtag cttgggaagt cgtaacggtt caaaaaggaa atggtcaccg
     1921 cattaataaa ccagataaaa aggacgagaa ggaagagcaa cctagcaaag aaattaccaa
     1981 aaatatcgaa caatcaaaca cacttgagca aactactttg ccacctgtac gccaaacgct
     2041 gactgacaaa aaagtagagc aaaatgcaga gccatcaaaa gaagaaacgg tttctatttt
     2101 tgatgattcg aaaaaagata tgcctactaa gcaagagaaa atggctcgta ctgtaagaga
     2161 caaagggaca aaaggaaacg tatctgtgca tgatagtggt gagaataccc aagttcaaag
     2221 cctgcctaag accggtgaaa aaacgagctt ggttgccaat ataatgttat cgatcatatt
     2281 attcttattt gctttattca ttggcaagaa aaagataaca gaaagtgagt aaaaggcgat
     2341 gacaaagctc tctcaaaaat tcgtttgcga cagatgagag gcattgtgta gctttaaatt
     2401 aaaagagtta aaatttttct ttcaatgaat tatttgctta tagaggtgaa agtagagttt
     2461 gaaga
//
LOCUS       DBNFCK010000167         2453 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.168, whole genome shotgun sequence.
ACCESSION   DBNFCK010000167 DBNFCK010000000
VERSION     DBNFCK010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.168"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            26..1309
                     /gene="murA"
                     /locus_tag="AC1PIW_001845"
     CDS             26..1309
                     /gene="murA"
                     /locus_tag="AC1PIW_001845"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIP6606738.1"
                     /translation="MDTIIITGSKNGLKGQVEIEGAKNAVLPLLAATVLASQGQTKLT
                     QVPILSDVYTMNNVVRGLGVRVKFDEETKTIDIDATGKLGSETPYKYVSQMRASIVIL
                     GPILARNGYAKVSMPGGCTIGSRPIDLHLKGLQAMGAKIKQRAGYIEASAENLQGAHI
                     YLDFPSVGATQNLMMAATLAQGTTVLENAAREPEIVDLAIFLNKMGAKVKGAGTETIT
                     ITGVEELTGAEHQVVQDRIEAGTFMVAAAMTGGDVLVKDAVWEHNRPLISKMLEMGVE
                     VTEEAEGIRVRSQVDKLKPVTVKTLPHPGFPTDMQAQFTALMAVAKGESTMIETVFEN
                     RFQHLEEMRRMDLHSEILRDTAIITGGLALQGAQVMSTDLRASAALILAGLVAEGETI
                     VGKLTHLDRGYYRFHEKLAALGATIERVSGEDENG"
     gene            1302..1493
                     /locus_tag="AC1PIW_001846"
     CDS             1302..1493
                     /locus_tag="AC1PIW_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIP6606739.1"
                     /translation="MDKDNLSYVGKNLILVAVVLLIAILVFILGLMVGYGVIGDGDNV
                     FAVLSPAKWQELIGKFTGK"
     gene            1523..2362
                     /locus_tag="AC1PIW_001847"
     CDS             1523..2362
                     /locus_tag="AC1PIW_001847"
                     /EC_number="3.1.30.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HIP6606740.1"
                     /translation="MAKKNVKKQAMSLLSLLATIVVVAGGYLLSDNKSSNVADYSYYV
                     NQSKASEGTPNQELASSVLTEDVKKQLGNSIEWNSAGAFIINGNQTDLDASVASVPYG
                     DNKTKTVQGETVPTVANALLAKSTRQYKDREETGNGRTSWTPAGWHQIHDLTGEYDHA
                     VDRGHLLGYALIGGLSGFDASTSNPKNIAVQTAWSNQANDQTSTGQNYFESQIRRALD
                     KNKRVRYRVTLVYAESEDLVPVGSHLEAKAADGSLEFNVFVPNVQSGLSINYHSGEIA
                     VNQ"
BASE COUNT          731 a          399 c          631 g          692 t
ORIGIN      
        1 gatttaagta agaaaaggat aagaaatgga tacaattatt attacaggta gtaaaaacgg
       61 tttgaaagga caggttgaga ttgagggggc gaagaatgcc gttctgcctt tattggcagc
      121 aacagttcta gcaagtcaag ggcaaaccaa attgacccag gttcctattt tgtctgatgt
      181 ctatacaatg aataatgttg tacgtggtct gggagttcgt gtgaaatttg acgaggaaac
      241 aaagactatc gatattgatg caacaggtaa acttggtagt gaaactcctt ataagtatgt
      301 gagtcaaatg cgcgcctcta ttgttattct tggtccgatt ttggcacgca atggctatgc
      361 taaggtttct atgccaggag gttgtacaat tggtagtcgt ccaattgatt tgcacttaaa
      421 aggtttgcaa gctatggggg ctaaaattaa acaacgcgct ggttatattg aagcgagtgc
      481 agagaacttg cagggagctc atatctatct tgatttccca agtgttgggg caacgcaaaa
      541 tttgatgatg gctgcaactc tggcgcaggg tacaacggta ttagaaaatg cagcgcgaga
      601 acctgaaatt gttgacttgg ctattttcct aaacaaaatg ggggcaaaag ttaagggggc
      661 aggtacagag acgattacga ttactggtgt agaagaattg actggcgcag aacatcaagt
      721 ggtgcaagat cgtatcgaag cgggtacctt tatggtggca gcggcaatga ctggtggaga
      781 tgtcttggtc aaggacgctg tctgggagca taaccgtcca ctcatttcta agatgttgga
      841 aatgggagtt gaggtgacgg aagaagctga agggattcga gttcgttcgc aagtggataa
      901 gcttaaacct gtaacagtaa aaaccttgcc ccatccagga ttcccaacgg atatgcaggc
      961 tcaatttaca gctctaatgg cagttgcaaa gggtgaatct accatgattg aaacggtatt
     1021 tgagaatcgt ttccagcatt tggaagaaat gcgtcggatg gatttgcatt cggaaatttt
     1081 acgagataca gctatcatca cgggaggact tgctctgcaa ggtgctcagg ttatgtctac
     1141 tgatttacga gcaagtgccg ctttgattct ggctggtttg gtcgctgaag gtgaaacgat
     1201 tgtaggtaaa ttaacgcact tagatcgagg ttactatcgt tttcatgaga aattagctgc
     1261 tttaggtgca acgattgaac gtgtgagtgg agaagatgaa aatggataag gataatctta
     1321 gctatgttgg taaaaattta atcttagtag cagttgtttt gttgattgct attttggtgt
     1381 ttattttagg attaatggtt ggttacggtg tcataggaga tggagacaat gtatttgcgg
     1441 tgttgtcacc agctaagtgg caagaattaa tcggtaaatt tacaggaaag taggctggac
     1501 ctgctttttt gatggagaga aaatggcgaa gaaaaatgta aaaaaacaag caatgagtct
     1561 gctcagctta ttggctacga ttgttgtagt agcgggtggc tatcttttaa gtgataacaa
     1621 gagtagcaac gttgctgatt attcctatta tgtcaatcag tctaaggcaa gtgaaggcac
     1681 acctaatcag gaactggcta gttcggtatt gacagaagat gtaaaaaagc aactgggaaa
     1741 ttcgattgaa tggaatagtg ctggagcgtt tattattaac gggaatcaaa cggatttaga
     1801 tgcatcggtt gccagtgttc cttatggtga taataagaca aaaacagtcc agggagaaac
     1861 ggttccaaca gtagccaatg cgcttttagc taaatctaca cgtcagtata aggatcgtga
     1921 agaaacagga aatggtcgaa cttcttggac acctgcgggt tggcatcaga tccatgattt
     1981 aactggagaa tatgaccatg cagtggaccg tggtcacctc ctaggttatg ctttgattgg
     2041 tggattgtct ggcttcgatg cttcaaccag caatcctaag aatattgcag ttcaaacagc
     2101 ttggtccaac caagctaacg accaaacttc aactgggcag aattattttg aaagtcagat
     2161 tcggcgggcc ttggataaaa ataagcgtgt ccgctatcgt gtgaccttgg tgtatgctga
     2221 gagtgaagac cttgttcctg tcggaagtca tctagaggca aaagcagcag atggtagcct
     2281 agagttcaat gtgttcgttc caaatgtgca gagtggtctc agtatcaact atcatagtgg
     2341 ggaaattgca gtaaatcaat aaatagtcag acgaattctc cctcgttaaa tcaaatatat
     2401 cagacgattt tcacgtaaaa tcgtcttttt ttgcgtataa ttaaaaatat tta
//
LOCUS       DBNFCK010000168         2389 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.169, whole genome shotgun sequence.
ACCESSION   DBNFCK010000168 DBNFCK010000000
VERSION     DBNFCK010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.169"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..1677
                     /gene="ezrA"
                     /locus_tag="AC1PIW_001848"
     CDS             <1..1677
                     /gene="ezrA"
                     /locus_tag="AC1PIW_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HIP6606741.1"
                     /translation="IIAYVACLIVRKRNDNLLVALEERKEELFNLPVNEEVETVKALH
                     LIGQSQVSFREWNQKWVDLSLNSFADIENHIFEAEGYNNAFRFVSAKNAIDSIDSQID
                     LIEEDIASIRQGLMELKEQEEKNSGRVKHALNLFDSLQEAVRENPDSYGETLSELEKQ
                     LKNIEVEFSEFVMLNSSGDPIEASEILDKTEEHMIALNQIMDRIPSLIERVTKDFPEQ
                     LEDLESGYRKLVEQNYLFTEANIESQFQNIRVSIRENTALIVSFDLDAAEAENEGIQA
                     KIDHLYKVFNREIEANKEAVKISKNLPKFLEHVVQNTQLLDEESQRLNATYLLADSKL
                     SRINQLKARLESIEIVVTESVEDIENPQVAYSILEERLDHSLASLKEIEEEQLVLADY
                     LKSQELSENTARKKATLYINKLHTLKRYMEKRNLPGIPAEFLTNFFRTSDHVEALIAE
                     LDYKRINIEVVNRLLENATYDMNQLEELAYLIVQNATLTEQLLQYSNRYRSFDESVQK
                     AFNRSLSIFEKDFDYQAAFEEISFALETVEPGVTERFVRSYEKTREAIRY"
     gene            1729..2364
                     /gene="serB"
                     /locus_tag="AC1PIW_001849"
     CDS             1729..2364
                     /gene="serB"
                     /locus_tag="AC1PIW_001849"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HIP6606742.1"
                     /translation="MTTGLLVMDVDSTLILEEGIDLLGEEAGLGAQVAAITERAMRGE
                     LDFEEALRERVALLKGLPVSVFDRIIKKIHFTPGAAELVSELKMRGYKVAVVSGGFHE
                     TVDRLAAQLELDYVRANRLEVVDGVLTGQVLGEIVTKDTKKACLEEWAAENGLSLSQT
                     IAMGDGANDLPMIQRAGIGVAFCAKPIVQEQVPYQINEKNLYKLIEILDSK"
BASE COUNT          785 a          381 c          538 g          685 t
ORIGIN      
        1 atcattgcct atgtagcttg cctgattgtt cgtaaacgaa atgacaacct tttggttgca
       61 ttggaagaac gtaaggaaga gctatttaac cttccagtta atgaagaagt cgaaactgtc
      121 aaagcacttc atttgattgg tcaaagtcag gtgtcattcc gtgaatggaa tcaaaaatgg
      181 gttgatttgt cgctcaattc atttgccgat attgaaaatc atatctttga ggcagaaggc
      241 tataacaatg cctttcgctt tgtgagtgca aagaacgcta tcgacagtat cgacagccaa
      301 attgatttaa ttgaagaaga tattgcctct atccgtcaag gtttgatgga gttgaaggag
      361 caagaagaga aaaactctgg tcgcgtaaaa catgcgttaa acttgttcga tagtttacag
      421 gaagctgttc gtgaaaatcc agatagctac ggtgagacac tatcagaact tgagaaacaa
      481 ttgaaaaata ttgaagttga gttttctgaa tttgtgatgc ttaattcctc aggtgacccg
      541 attgaagctt ctgaaatcct tgataagact gaagagcata tgattgccct caatcaaatc
      601 atggatcgca ttccaagttt gattgaacga gttacaaaag atttcccaga gcaattggaa
      661 gatttggagt cgggttatcg taaactggtt gagcagaatt atttgttcac agaagctaac
      721 atcgagtcac agttccaaaa tattcgcgtt tcaattcgtg aaaatactgc tttgattgtt
      781 tcctttgatt tggatgccgc agaagctgaa aatgagggaa ttcaagcaaa aattgatcat
      841 ttgtataaag tcttcaatcg tgaaatcgaa gcgaataagg aagctgttaa gattagtaaa
      901 aacttgccaa aattcttgga gcatgttgtc caaaatacac agctcttgga tgaggaaagc
      961 caacgcctaa atgcaactta cttgctggca gatagcaaac tttcacgtat caatcagctt
     1021 aaagctcgtt tggaatcgat tgagattgtt gttacagaaa gtgtggaaga cattgaaaat
     1081 ccacaagttg catattctat tttagaagag cgtttggatc attcattggc aagcttgaaa
     1141 gaaatcgaag aggagcagct tgttttagct gactatctta aatcacaaga attgtcagag
     1201 aacacagcgc gtaagaaagc tacactttat atcaacaaat tgcataccct taagcgttat
     1261 atggaaaaac gcaaccttcc aggaattcca gctgaatttt taacaaactt ctttagaacg
     1321 agcgatcatg tggaagcctt gattgccgag ttagactata aacgaatcaa tatcgaagtt
     1381 gttaaccgtt tgttggaaaa tgcaacgtat gatatgaatc aattggaaga gttggcgtat
     1441 ttgattgtgc aaaatgcgac tttgacagag cagttgctac aatactctaa ccgttatcgt
     1501 tcattcgatg aaagtgtgca aaaagcattt aaccgttcat tatcaatttt tgaaaaggat
     1561 tttgattacc aagcagcatt tgaagaaatt tcatttgctc tggaaacagt tgaacctggt
     1621 gtaacagagc gttttgtgcg ttcatacgaa aaaactcgcg aagccattcg ctactagata
     1681 aaaagccatt tggcttttta tttttataca ttgaaaggaa tattggagat gacaactgga
     1741 ttattagtta tggatgttga ttcaacatta attttggaag aaggaataga tttacttgga
     1801 gaagaggccg gtttaggagc gcaagttgca gcaattaccg aacgtgctat gcgtggggaa
     1861 ttggattttg aagaagcctt acgtgaacgt gtggcgttgt taaagggatt gcctgtttct
     1921 gtgtttgatc gaattatcaa aaaaatccac tttacgcctg gagcagcaga attagtctca
     1981 gaattaaaaa tgcgaggcta taaggtagca gtcgtttcag gcgggtttca tgaaacagtt
     2041 gatcgactag ctgcacagtt agagttagac tatgttcggg ctaatcgatt agaagtagta
     2101 gatggagtat tgacgggaca agtgttgggt gaaattgtaa cgaaagatac taaaaaagca
     2161 tgtttagaag aatgggcggc agaaaatggg ctgagtctat ctcaaacgat tgccatgggg
     2221 gatggagcaa atgatctacc aatgattcaa cgagcaggaa ttggagtagc tttctgtgct
     2281 aaaccgattg tccaagaaca ggtgccctac caaataaatg aaaaaaatct ctataaacta
     2341 atagagattt tagatagtaa ataatgggag cgggaaagaa ctcgactaa
//
LOCUS       DBNFCK010000169         2376 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.170, whole genome shotgun sequence.
ACCESSION   DBNFCK010000169 DBNFCK010000000
VERSION     DBNFCK010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.170"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..770)
                     /locus_tag="AC1PIW_001850"
     CDS             complement(<1..770)
                     /locus_tag="AC1PIW_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P 5-aminopentanol modification-associated
                     protein YfmF"
                     /protein_id="HIP6606743.1"
                     /translation="MKLQEGVDLHFIDTDQFTTNRIRIRFAAEMSEATVAGRVLVANI
                     FEMGNQEFQTAQAVRRRLAELYGAQFSTSVSKRGRVHCVDVTISYVSPRHLPENEDIT
                     VEILDFLYTCIFRPLKKGRGFDSQIFEVEKTNLINFLQSEIEDNFYHVDVEMSKLFYK
                     DPSLQIPRVGRLDLVEKETAESTFQIYRNMLRMDKIDIFVLGKVDREQVKRKLEDFGF
                     TYRNPKLELEYHQEYSNITQEKIERKQARQSILELAYH"
     gene            844..1227
                     /gene="yaaA"
                     /locus_tag="AC1PIW_001851"
     CDS             844..1227
                     /gene="yaaA"
                     /locus_tag="AC1PIW_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HIP6606744.1"
                     /translation="MEFKLFDDYITLQALLKTTGILHSGGAIKGFLEENTILFNGEDE
                     KRRGKKIRVGDVITLPDHNTSITIVAPSAEEIKQHQEDQAEKERVAAIVKKLNQENKN
                     NKKQVKTPKKATKKTERKPVRFPGM"
     gene            1230..2324
                     /gene="recF"
                     /locus_tag="AC1PIW_001852"
     CDS             1230..2324
                     /gene="recF"
                     /locus_tag="AC1PIW_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638862.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HIP6606745.1"
                     /translation="MWLERLELQHFRNYNQLDIEFHKGLNVFLGENAQGKTNILESIY
                     VLALTRSHRTRTDKDLLQFQEKELSISGLLHRTSGKVPLDIHLTDKGRVTKVNHLKQA
                     KLSNYIGHMNVVLFAPEDLQLIKGAPALRRKFIDVELGQIKPLYLSDLSNYNHVLKQR
                     NTYLKSTDKIDENFLSVLDQQLAEYGSRVIQHRIDFLKKLEEFGNRKVQEISGNREEL
                     TIEYQTSIELTDDVNLIDKFLTELEKSRKRDLFKKNTGVGPHRDDVAFFINGMNAHYA
                     SQGQHRSLVLSLKLAEIELMKEVTREYPILLLDDVMSELDNNRQIKLLETITDTIQTF
                     ITTTSLDHLHKLPDSLKIFHIESGKVTESE"
BASE COUNT          815 a          479 c          395 g          687 t
ORIGIN      
        1 aaatgatatg ccaattccaa aatggactgc cttgcctgtt tacgctcgat tttttcttgc
       61 gtgatatttg agtattcctg atgatattct aactctaatt ttggatttct ataagtaaaa
      121 ccaaaatctt caagttttct ttttacttgt tctctgtcaa ccttccctaa gacaaatata
      181 tcaattttat ccatacgcaa catattccga taaatctgaa aggttgattc tgctgtttct
      241 ttttcaacca aatcaagcct accgacgcgt ggaatttgaa gagagggatc tttataaaaa
      301 agcttactca tttcaacatc tacatgataa aaattatctt ctatctctga ctgaagaaaa
      361 ttgattaaat tcgttttttc aacctcgaaa atctggctat caaatcctcg cccctttttc
      421 agtggtctaa atatacatgt gtataaaaaa tcaagaatct ccactgtaat atcctcattt
      481 tctggtaagt gacgaggact gacatatgaa attgtcacat ctacacagtg caccctacca
      541 cgtttcgaaa ctgaggtcga gaactgagca ccatacaatt ctgccaatct tctccgaaca
      601 gcctgagcag tctgaaattc ttggttaccc atttcaaaaa tatttgcaac taacacacga
      661 ccagcaactg tagcctcact catttcagct gcaaagcgaa tacgtatacg atttgtcgta
      721 aactgatctg tatcaataaa atgaagatct actccctctt gtaatttcat cgtcttcctc
      781 tttcaaacat tgtaccttcc attataccat tttgtgatat aatattctgt aacgaggtga
      841 cttatggaat ttaaattatt tgatgactac atcacattac aagcattgtt aaaaacgact
      901 ggtattctcc attcaggagg tgccattaaa ggtttcttag aagaaaatac cattcttttc
      961 aatggagagg atgaaaaaag acgcggtaaa aaaatccgtg tcggagatgt tatcacactc
     1021 ccagaccaca acaccagcat cacaatcgtc gctcctagtg cagaggaaat caaacagcac
     1081 caagaggatc aagctgaaaa agaacgtgtg gctgcaatcg ttaaaaagtt aaatcaagaa
     1141 aataaaaata ataagaaaca ggtaaaaact cctaaaaaag caactaaaaa gactgaaaga
     1201 aaacctgttc gtttccctgg tatgtagaca tgtggctaga acgattagag ttacaacatt
     1261 ttcgcaatta caaccaatta gatattgagt tccacaaagg gctcaatgtc tttttaggcg
     1321 aaaacgccca aggaaaaacc aatattctag aatctatcta tgttttagcc ttaacacgaa
     1381 gtcatcgaac acggacagat aaggacctac tacagtttca agaaaaggaa ttatctattt
     1441 ctggtttact ccatcgcaca agcggtaaag ttccccttga cattcacttg acagacaagg
     1501 gacgagtgac caaggtcaac catctcaaac aagccaagct ttccaactat atcggccaca
     1561 tgaatgttgt tctcttcgcc cctgaagatt tacagctgat aaaaggtgcg cctgctttga
     1621 gaagaaagtt catcgatgtc gagctcgggc aaatcaagcc actctatctc tctgacctat
     1681 cgaattataa ccacgtttta aaacaaagaa atacttacct caaatctaca gataagattg
     1741 atgagaactt cctatctgtg ttagatcagc aacttgctga atatggtagt cgtgttatcc
     1801 aacatcgtat tgactttcta aagaaattag aggaattcgg aaatagaaaa gttcaagaaa
     1861 tatctggcaa tcgggaagaa ttgacaatcg aataccagac ctcaatcgaa ttgacagatg
     1921 atgtcaattt aattgacaaa ttcttgacgg aactagaaaa gtctcgtaaa cgtgatttat
     1981 ttaagaaaaa tacaggagtt ggaccacatc gagatgatgt agctttcttt ataaatggaa
     2041 tgaatgccca ttatgctagc caaggtcagc accgcagtct cgttctgtca ctcaaactag
     2101 ctgaaattga gctgatgaaa gaagtcacac gagagtaccc aattctactc ttagatgatg
     2161 ttatgagtga gctggataat aatcgtcaaa tcaaactatt agaaactatt acagatacga
     2221 ttcaaacatt tattacaacg acatctttag accatttaca taagctacct gatagtttaa
     2281 aaatcttcca tattgagtct ggtaaggtta ctgagtcaga ataagattgt ttacactatc
     2341 gtcaataaag tttacaaaaa agtaaagacc ctgtca
//
LOCUS       DBNFCK010000170         2359 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.171, whole genome shotgun sequence.
ACCESSION   DBNFCK010000170 DBNFCK010000000
VERSION     DBNFCK010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.171"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            141..842
                     /locus_tag="AC1PIW_001853"
     CDS             141..842
                     /locus_tag="AC1PIW_001853"
                     /EC_number="3.4.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HIP6606746.1"
                     /translation="MWSPKKLTVLKWFDILILTIILFGDGIINSTLQYIALQNQTTTL
                     QENLTFSTMDNYKALALQLVWLTIAIFYLLLRNFDFSIWKKHIFITPWVPLQAVALFI
                     FSALCLDIYNLVSYQFLASNTPSMFQLFPNIDLSLILYSLLNGFYEEIFFLNLCLLVN
                     PKYAKWAFLYSLIIRCSFHTYQGLLSALGLGLILGTIFYLLYQKIKPKNLLPFFLAHA
                     VADVIGLTILSYILY"
     gene            complement(1307..>2359)
                     /locus_tag="AC1PIW_001854"
     CDS             complement(1307..>2359)
                     /locus_tag="AC1PIW_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIP6606747.1"
                     /translation="VRYSLNNDREKTAKYSSSKVSLNHETKKVLVPVPIEPKDTDKVV
                     DKSTGISKVENMTPMVTPEMPKQTEPNVPDSTLPMQPELPKETGKPGNNVETESPKPI
                     ENSTDDSANKPDSTTPKTTDNIIIPNPTLPMKPDSTEETEKPGNNVEPESPKIIEDST
                     DDSANKPDSTIPKATDNIIIPNSTFPMKSESPKEIGETENSVKPESPKPIEDTDSSAG
                     TQKLDIPKVMDTPDLSRPMEPESTEEIGKAENSDTPDSSKTIKDSDNSAPISTIKDKV
                     NAETVANQVELAMNQKSRLSDNVEKVSSVNRSTEKSNSLPSTGQADSPIYTLTALSLL
                     VGAQVLYRTKRKQESK"
BASE COUNT          663 a          426 c          397 g          873 t
ORIGIN      
        1 aatagagcac catgttctgc tatttcagct ttcttttgac ttcttttgtt taaaacagat
       61 aaaaaacaga cgaaaccgag agcatatata gatttgtttg ataaactgaa ctaaacaaaa
      121 atgaaaggaa ctatcccatt atgtggagtc caaaaaaatt aaccgtatta aaatggtttg
      181 atattctcat tctaacaatt attttatttg gagatggaat catcaactct actctccaat
      241 acatagcctt acaaaatcaa actacaactc ttcaagaaaa tttgacattt tcaacaatgg
      301 ataattataa ggctttggct ctacagttag tttggctcac aatagccatc ttctatctat
      361 tactccgaaa ttttgatttc tcaatttgga aaaaacatat ttttattact ccttgggttc
      421 cattacaagc tgttgcctta ttcatttttt ctgcattatg cttggatatc tataatctag
      481 tatcctatca gtttctagct tccaacactc catccatgtt tcaactattt cctaacattg
      541 atttatcact gatcttgtac tcattgttga acggatttta tgaagaaatt ttcttcctta
      601 acttgtgctt actggttaac cctaaatatg caaaatgggc atttttatat tcgttaatta
      661 ttcgatgtag tttccatacc tatcaaggtt tacttagtgc actcggttta ggcttaattc
      721 taggaactat tttctatcta ctttatcaaa aaataaaacc taaaaattta ttaccatttt
      781 tcctagcaca tgctgtggct gatgttatcg gtttaactat tctatcatat attctatact
      841 agttatctaa cagataattt gaacacaagc tatcctcata tcaccaaaga ctgagtggac
      901 ccctactagt catgaccacc cgtgaaaacg ggtggtttat tttgtgtaat ttaggaaaag
      961 agtgccaaaa aacgcataaa atcgtgttgc taagaacgtt gattttatgc gttttattat
     1021 atgacttatc tcaatatttt tattgaaatt tatatttatg cccaactata cttctcagaa
     1081 tcaatgtcca tatctcatct cagcctagta tagattgaca gatttattct acattcccag
     1141 ttcagcctct gcagctattg cgaatggctc gttttatatt caatctccaa agatttccta
     1201 aaacttggat ctgtacggag ggtaaaacaa ttccatcact attataggca taatctctac
     1261 tctattaacc atatgtatat acaagtagaa attacacgac caacatttac ttcgattctt
     1321 gcttacgttt tgtacgataa agtacctgag ctcctacaag aagagagagg gcagttaacg
     1381 tatagattgg agaatcagct tgtccagtag atggaagcga gtttgacttc tcggttgaac
     1441 gattcacact agatactttc tccacattgt cagaaagacg tgatttctga ttcatagcaa
     1501 gctctacctg atttgccacc gtttcggcat tcactttatc cttgatagta gaaattggag
     1561 cagagttatc tgaatcttta atagtcttag aagaatctgg agtatcacta ttttctgctt
     1621 taccaatctc ttcagttgac tctggttcca ttgggcgaga caaatctggg gtatccatta
     1681 cttttggaat atccaatttt tgtgtacctg ctgaactgtc tgtatcttcg ataggcttag
     1741 gtgattctgg tttaacactg ttttctgttt caccaatttc tttaggtgac tctgacttca
     1801 ttggaaaagt ggaatttgga ataataatat tgtccgttgc tttcggaatt gttgagtctg
     1861 gtttatttgc tgaatcatcg gttgagtctt cgataatctt aggtgattct ggttcaacat
     1921 tatttcctgg tttttcggtc tcttcagttg aatctggctt cattggaaga gtgggatttg
     1981 gaataataat attgtccgtt gttttcggag ttgttgagtc tggtttattt gctgaatcat
     2041 cggttgagtt ttcgataggc ttaggtgatt ctgtctcaac attattacct ggttttccag
     2101 tctctttagg taactctggt tgcattggaa gagttgagtc tggaacattt ggttctgtct
     2161 gtttaggcat ttcaggtgtc accataggtg tcatattttc tacttttgat atacctgttg
     2221 acttgtctac caccttatct gtatcttttg gctcaatagg aacaggaacc aaaactttct
     2281 tagtttcatg gttaagagac acttttgagg atgagtattt tgctgttttt tctctatcat
     2341 tattaagaga ataacgaac
//
LOCUS       DBNFCK010000171         2359 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.172, whole genome shotgun sequence.
ACCESSION   DBNFCK010000171 DBNFCK010000000
VERSION     DBNFCK010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.172"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            1..1209
                     /locus_tag="AC1PIW_001855"
                     /pseudo
     CDS             1..1209
                     /locus_tag="AC1PIW_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719507.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
     gene            1250..1948
                     /locus_tag="AC1PIW_001856"
     CDS             1250..1948
                     /locus_tag="AC1PIW_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIP6606748.1"
                     /translation="MYKILVVDDDFEIVKLMRTILEMKNYNVTTYQEVRLPIKISDFD
                     GFDLILLDVMMPNVDGLQICKQIRKSVSSPIIFVSAKDSEDDIVSGLNLGGDDYITKP
                     FSVNQLVAKVAAHLKREERYRNAESNESVIRELFPLTFYLQEKLVCVNGEAISLTKRE
                     YDILELLSSKTNKVYTREEIYECVYDDEADALFRSISEYIYQIRVKFGPYGINPIKTI
                     RGMGYKWNDEKVFN"
     gene            1929..>2359
                     /locus_tag="AC1PIW_001857"
     CDS             1929..>2359
                     /locus_tag="AC1PIW_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIP6606749.1"
                     /translation="MKRYSINKRILRTVLEIVIGTVANVIMLLVLSYVLIFTGQLNTY
                     GTDYHISISDSKSANGIKNQMDYSVFDYVLFDSDNGELVSGNYQKQDLQHYKEVFDTG
                     SSVSEGSILFTKYENSDFILVIRRPTMPEFVNRNFRHISYN"
BASE COUNT          838 a          283 c          462 g          776 t
ORIGIN      
        1 caaagtcaag gaattaaagt tactgataaa atacctgaaa taaattttta aactatttct
       61 accgaaaaaa gtgaagatac aataaaaaaa gaaaactcta attatattga tgacatagtt
      121 atagataata ctatgactgt aaagcctaat attacttatt tgtatgggga caaggtttat
      181 acaaatacta atttagattt ttggggaaat cagtgggata tgcaaaagag tattagtact
      241 ggaagtaaat ttcaacataa gtcttctggt aaagcaacaa ttggtgtaat agattctggt
      301 attacatatg ataatccaga tatttattca cacattattt cagtacaaaa tttcacatca
      361 gatttggaaa caggaatagt tgataaccaa aatgttgtag ataaaattgg ccatgggacc
      421 tctgttgtgg gacaaatttc atcaagtgga gaatatttag ggatagcacc tgacatgaaa
      481 atacgaatgt atcgtgtttt tgatgaaggg aatgctcaag accaatggat tctcaaagcg
      541 ctcattcaag cagcaaaaga tgatgttgat gttattaacc ttagtttagg agaatatttg
      601 ttaagagatt ccactgatga agatgatgat acagctttga tcaatatcta tcaacgggca
      661 attaattatg ctcataatca aggttccgtt gtcgttgctt cagtaggtga tgaagggatg
      721 gatttacaga atcaagatga attaaaaaac tatcttggta gtttaagagg aaaagattat
      781 agtcaagttg atggaactgt acaagatatt ccggctgaat tagacaatgt tgttacagtt
      841 ggttcagtag atgataatga tcttgtatct agtttctcaa atcaagggaa tggcgacatt
      901 gatatctatg caacaggagg tggaagtcaa gcgctttcta aagtaggata tgataaatgg
      961 gtagctgata aattatatga aaaagaatgg attcttgttc caacgcttga aggaaagtat
     1021 acatatgcat atgggacttc aatttcgact cctaaagtct ctgcagcttt aggattgatt
     1081 atagaaaagt ataatttgaa ggaccaacca gatgaagcta ttcgaatttt atatgataat
     1141 tgttttctta gtaataatgg aaacaatgga caaattaggc tactaaatat tacaaatttt
     1201 gtgcaataat agtgtaaaat agtagtatga gataaaagag gtacctagta tgtataagat
     1261 tttggtagtt gatgatgatt ttgaaattgt aaaattgatg cgaacaattt tagaaatgaa
     1321 aaactataat gtgacgactt atcaagaagt taggcttcct ataaaaataa gtgattttga
     1381 tggatttgat ttaatattgc ttgatgttat gatgccaaat gttgatggtc tgcaaatctg
     1441 taaacagatt cgtaaatcag tatcctctcc gattattttt gtcagtgcaa aagattctga
     1501 agatgatatt gtatctggct taaatctcgg tggtgacgat tatatcacaa agccatttag
     1561 tgttaatcaa ctagttgcta aggttgcagc gcatttaaaa cgagaagaac gttatagaaa
     1621 tgctgaatct aatgaatctg ttattcgaga attatttcca ctaacatttt accttcaaga
     1681 aaaactcgtg tgtgttaatg gtgaagctat ttctcttact aaaagggaat atgatatttt
     1741 agagctatta tctagtaaga caaataaggt ttatacaaga gaagaaatct atgaatgtgt
     1801 ctatgatgat gaagcagatg cattatttcg ttcaatttcg gaatacattt atcaaattag
     1861 agtcaagttt ggtccctatg gaattaatcc cattaaaaca ataaggggga tgggatataa
     1921 gtggaatgat gaaaaggtat tcaattaata agcggatttt aaggacagtt cttgaaatag
     1981 tgattggtac agttgcaaat gttattatgt tgcttgtatt gagctacgtt cttattttta
     2041 caggtcagtt gaacacatat ggaacagact atcatatttc aattagtgat agtaaatctg
     2101 ctaacggtat taagaatcaa atggattatt ctgtctttga ttatgtattg tttgatagcg
     2161 ataatggcga attggtctct ggaaattatc aaaaacagga tttacaacat tataaagaag
     2221 tttttgatac tggaagttcg gttagcgagg gttctatatt gtttacaaaa tatgaaaact
     2281 cagattttat tttggttatt agaagaccaa caatgcccga atttgtaaat cgaaactttc
     2341 gacatatttc ctataattt
//
LOCUS       DBNFCK010000172         2292 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.173, whole genome shotgun sequence.
ACCESSION   DBNFCK010000172 DBNFCK010000000
VERSION     DBNFCK010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.173"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            85..888
                     /locus_tag="AC1PIW_001858"
     CDS             85..888
                     /locus_tag="AC1PIW_001858"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIP6606750.1"
                     /translation="MKKILVLALASSILLTACTNNSTANSSSSTASSSSPSSTTTADT
                     TASSQYAKDLAYAINNPKATFPQLSNEIGENEAAIKIKTTEGDITIKLFPEQAPLTVE
                     NFLTHAKEGYYNGTIFHRVIKDFMIQGGDPLGNGTGGESIWAGKDTTIDAGNGFKDEI
                     SAFLYNIRGSLSMANAGAGTNGSQFFINQNTTDMSSQLSSSSYPGKIIDAYKNGGNPN
                     LDGKHTVFGQVIEGMDIVDKIASVETDNSDKPKTDVKIESIEILKDYTF"
     gene            1017..2273
                     /locus_tag="AC1PIW_001859"
     CDS             1017..2273
                     /locus_tag="AC1PIW_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HIP6606751.1"
                     /translation="MEQLNLITNFLRIKDKNITITDEYDMGTHLELHGYLDYMAPKCP
                     ECKGQMAKYDYQKASKIPYLETAGYPLLIRLKKRRFKCKECGKMAGAETPLVKKNHQI
                     SVAVSQKIAQLLIENQAMTHIAHRLSISTSSVMRKLNEFKFETDWNTLPEVMSWDEYA
                     FKKGKMSFIAQDFDSLNVIAILDGRTQATIRNHFLRYPRKVRNQVKVITMDMFSPYYQ
                     LAKQLFPNAKIVLDRFHIVQHLGRAMNRVRIQIMNQFDRTSQEYRALKRYWKLIQQDS
                     RKLSDKRFYPPMFRMHLTNKEILEKLLSFSEELRQHYELYQLLLFHFQEKNSDHFFDI
                     VELEIATVNPIFQTVFKTFLKDKDKVLNALELPYSNAKLEATNNLIKVIKRNAFGFRN
                     FENFKKRILIALNIKKERTKFVLSRC"
BASE COUNT          776 a          486 c          412 g          618 t
ORIGIN      
        1 tttttatact agataaaaat tgaaaaaatc catatttcat ggtagaatga gcataatttt
       61 gaacaagaca agaaaggatt ttcaatgaaa aaaatactag ttcttgctct agcttcgagc
      121 atcttgctaa ctgcctgcac aaacaactcg acagctaata gctcatcttc tacagcatct
      181 agttctagtc caagctcgac tacgacagct gatacaactg cttctagcca atatgccaaa
      241 gatttggcat acgccattaa caatcccaaa gctacattcc cccaactttc caatgaaatt
      301 ggtgaaaatg aggctgctat taaaataaaa acaaccgaag gcgacatcac tataaaactc
      361 ttccccgagc aagcaccact aactgtggaa aactttctga ctcatgcgaa ggaaggatac
      421 tataatggaa cgatcttcca tcgtgtcatt aaggatttca tgattcaagg tggcgatcct
      481 cttggaaatg gtactggtgg agagtctatc tgggccggaa aagatactac tatcgatgct
      541 ggaaatggtt ttaaagacga gatttcagct ttcctctata acattcgtgg ctcactttct
      601 atggcaaacg ctggcgcggg aacaaatgga agtcagtttt tcatcaatca aaatacaaca
      661 gacatgtcta gccaactctc tagttctagc tatcctggta aaatcattga cgcttataaa
      721 aacggaggca atccaaactt agatggcaag cataccgtat ttggtcaagt cattgaaggg
      781 atggacatcg tggataaaat cgcttcagtc gaaacagaca atagcgataa acctaaaaca
      841 gatgtaaaaa ttgagtctat tgaaattctg aaagattaca ccttttaaaa atgggttcta
      901 taatttctgt agtgggtaaa accaccgtgg agattatgga gcctttttga gtatagaaaa
      961 aaagtcccat atgacctata atgaaaagca accacactca cattagaaag actcatatgg
     1021 aacaactaaa tcttatcaca aattttctca gaattaaaga caaaaatatc actatcactg
     1081 atgaatatga tatgggaact cacttagaac tccacggtta cttggattac atggctccta
     1141 aatgtccaga atgcaaagga caaatggcta agtacgacta ccagaaagca tcaaaaatcc
     1201 cttacctaga aactgctggt tatccactcc ttatccgcct taaaaagcgt cgtttcaagt
     1261 gtaaagaatg tgggaaaatg gcgggcgctg aaactcctct tgttaagaaa aaccatcaaa
     1321 tctccgtcgc tgttagtcag aagatcgctc aattactcat cgaaaatcaa gcaatgacac
     1381 atattgcaca caggctatct atctcaacat catcagttat gagaaagctc aatgagttca
     1441 agtttgaaac ggattggaat accctacctg aagtgatgag ttgggacgag tatgccttca
     1501 agaaggggaa aatgagcttc atcgctcaag atttcgactc cctaaacgtc atcgccattc
     1561 tcgacggaag aactcaagca accatccgaa accactttct acgctatcct agaaaggtta
     1621 gaaatcaggt caaagtgatt accatggata tgtttagtcc ctattatcaa cttgctaaac
     1681 agctttttcc gaatgcaaaa atcgtactcg accgctttca tattgtgcaa caccttggcc
     1741 gtgctatgaa ccgtgttcgt attcaaatta tgaatcaatt cgacagaacc tcgcaggaat
     1801 accgagcctt gaaacgctac tggaaattga ttcaacagga tagtcgtaaa ctcagcgata
     1861 aacgatttta tccccctatg tttcgaatgc atttgactaa caaggaaata ctggaaaaac
     1921 tcctgtcttt ctccgaggaa ctacgacagc actatgaact ctatcaattg ctcttattcc
     1981 atttccaaga gaagaactca gaccatttct ttgacatcgt cgaactggaa atagccactg
     2041 ttaatcctat tttccagacg gtatttaaga catttctaaa ggataaagac aaggttttga
     2101 atgccctaga attgccttat tccaacgcca aactggaagc tactaataat ctcatcaaag
     2161 tcattaagag aaatgccttt ggtttcagga actttgagaa ctttaaaaaa cgcattttga
     2221 ttgctttgaa cataaagaaa gagagaacga agttcgtcct ctctaggtgt tagcttttca
     2281 tctacccact ac
//
LOCUS       DBNFCK010000173         2278 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.174, whole genome shotgun sequence.
ACCESSION   DBNFCK010000173 DBNFCK010000000
VERSION     DBNFCK010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.174"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..266
                     /locus_tag="AC1PIW_001860"
     CDS             <1..266
                     /locus_tag="AC1PIW_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606752.1"
                     /translation="REQFSKYLNLFALSDNITALKELNVFDEWDSVLKTLNKPGTRWG
                     QVQKTTINLAFARYLDSNNFVSSINERSNDIRLNSYKDKTIPL"
     gene            325..1416
                     /locus_tag="AC1PIW_001861"
     CDS             325..1416
                     /locus_tag="AC1PIW_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HIP6606753.1"
                     /translation="MSISGIIAEYNPFHTGHKYLLEQAEGLKIVVMSGNFMQRGEPAI
                     VDKWTRAQMALEHGADLVVEMPFLVSVQSADHFAKGAISILHRLGVEKLVFGTEEMLD
                     YQKIADIYVDKSEEMENFVKNLPDNFSYPQKTQAMWQEFAGLTFTGDTPNHILALAYA
                     KGVAGTGIQLSPVQRQGAGFHSEEVETSYASAIAIRKGADQLDLVRDFLPSASLFEEA
                     TKVSWRNYFPLLRYQITTHPDLSQVFQVNEELASRIRSAIGSVATVEELVEAVTTKRY
                     TKARVRRVLTYILINAVETPLPDAVHVLGFSARGQAYLKQVKERVGLVTRIGKEPWDS
                     LTQQADLVYQLGADAMAEQTYGRVPVRVE"
     gene            1512..2231
                     /locus_tag="AC1PIW_001862"
     CDS             1512..2231
                     /locus_tag="AC1PIW_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIP6606754.1"
                     /translation="MKTVKEMSQLSGISVRTLHYYDEINLLTPSFIAENGYRYYDNEA
                     FEKLQEILLFRELEFPLKEIKKIVGNAAYDRESALKDQIRLLELKKKHLEKVIKHAKS
                     LQQKGENYMNFEVFDKSDLLAFQEEAKERWGNTAAFQEFSAKTNQEGFAHISAEMSGI
                     MMEFGKMKSLSADDSKVHKQVEVLKAYISENFYNCTNEILASLGQMYIADNRFTQFID
                     QVGGEGTASFVSQAIAVYCKE"
BASE COUNT          687 a          400 c          580 g          611 t
ORIGIN      
        1 agcgagagca gttttctaag tatttgaatc tatttgctct atcggataat attacagctt
       61 taaaggaact caatgtattt gatgagtggg atagtgtatt gaaaacatta aataaaccag
      121 ggactagatg gggacaagtg caaaaaacaa caataaactt ggcatttgca agatacttag
      181 atagtaataa ttttgtaagc tcaattaacg aacgaagtaa cgatatccgc ttaaatagct
      241 acaaggataa aactatacct ttgtagacct atgttagact atgatagagt tgtcgaatta
      301 aaatacaaaa aataggagaa atgcatgtct atttcaggga taatagcaga atacaatccc
      361 tttcacacgg ggcataaata cctgctggaa caggccgagg ggctgaaaat tgtcgtcatg
      421 tctggcaatt ttatgcaacg gggtgagcca gccattgtgg ataagtggac acgggctcag
      481 atggctttgg aacacggggc agatcttgtt gtcgagatgc cctttttggt gtcggtccag
      541 tcggctgacc atttcgccaa gggagcaatc agcattttac acaggctagg tgtggaaaaa
      601 ctggttttcg gtacagaaga aatgctggat tatcagaaaa ttgcggatat ctatgtggat
      661 aagtcggaag agatggaaaa ttttgtgaaa aacttgccag acaatttctc ttatccacag
      721 aagacccagg ccatgtggca ggaatttgcg ggtctaacct tcacaggtga tacgccaaat
      781 catattctgg ctctggctta tgctaagggg gtggctggaa caggcattca gctcagccct
      841 gttcaacggc agggagctgg ttttcattcg gaagaggtgg aaacgtccta cgcctcggcg
      901 atagctatcc gaaagggtgc tgaccagctg gacttggttc gtgacttttt accgtctgcc
      961 agtctctttg aagaggcgac caaggtaagc tggagaaact attttcctct gctccgctac
     1021 cagattacga cccacccaga tctcagtcag gtctttcagg tgaacgaaga actagccagt
     1081 cgtatccgct ctgccattgg gagcgtagcg accgtggagg agttagtgga ggccgttacg
     1141 accaagcgct acaccaaggc gcgggtgcgg cgggtgttga cctacatcct gattaatgcg
     1201 gtggaaactc ccttaccaga tgcggtccat gtgctaggct tctcggctcg aggtcaggcc
     1261 tatctcaagc aggtcaagga gcgagtgggc ttggtgacgc ggatcggtaa agagccctgg
     1321 gacagcctga cccagcaggc ggatttggtc tatcaactgg gcgctgacgc aatggcagag
     1381 cagacctatg ggcgcgtgcc ggtgagggtg gagtgagaat ctgagttgaa aatagaaaat
     1441 ttttataatt tttcccttga ctctaacgct gggtcataga ttatactgct atttatgaga
     1501 ggagattatc catgaaaaca gtgaaggaaa tgagtcagct ttcaggtatc agtgtgcgca
     1561 cacttcatta ttacgatgaa ataaatttgc tgacacctag ttttattgcg gaaaacggat
     1621 atcgttacta tgataatgag gcgtttgaaa agttgcaaga aatcttactt tttcgtgaat
     1681 tggaatttcc tctcaaagaa attaaaaaaa ttgtagggaa tgcagcttat gatagagagt
     1741 cagctttgaa agaccagatt agattactgg agttgaaaaa gaagcacttg gaaaaagtca
     1801 taaagcatgc caagtcttta cagcagaagg gagaaaatta catgaatttt gaagtgtttg
     1861 ataagtctga tttgttggct ttccaggagg aagctaaaga aagatggggt aataccgcag
     1921 cttttcagga gttttctgct aagacgaatc aggaagggtt tgctcatata agtgcagaaa
     1981 tgtctggtat tatgatggaa tttggaaaaa tgaagagttt atcagctgat gattctaaag
     2041 ttcataaaca ggtagaggtt cttaaagctt atattagtga gaatttttat aattgtacca
     2101 atgaaattct agcaagtcta ggacaaatgt atattgcaga taaccggttt actcaattta
     2161 ttgatcaagt gggaggagaa ggtacagcgt cctttgtcag tcaggcaata gcagtttatt
     2221 gcaaagaata aacgaaagaa gtctagtgag aactaggctt ttttagtatt atgaaaac
//
LOCUS       DBNFCK010000174         2246 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.175, whole genome shotgun sequence.
ACCESSION   DBNFCK010000174 DBNFCK010000000
VERSION     DBNFCK010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.175"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            219..1505
                     /locus_tag="AC1PIW_001863"
     CDS             219..1505
                     /locus_tag="AC1PIW_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HIP6606755.1"
                     /translation="MSKTLKRPEVLSPAGTLEKLKVAIDYGADAVFVGGQAYGLRSRA
                     GNFTMDEMREGIEYAHARGAKVYVAANMVTHEGNEEGAGAWFRELRDMGLDAVIVSDP
                     ALIVICATEAPGLEIHLSTQASSTNYETFEFWKELGLTRVVLAREVTMEEVAEIRKRT
                     DVEIEAFVHGAMCISYSGRCVLSNHMSKRDANRGGCSQSCRWKYNLYDLPFGEERRSI
                     KGEIPEEFSMSAVDMCMIDHIPDMIENGVDSLKIEGRMKSVHYVSTVTNCYKAAVDAY
                     LESPERFEAIKQDLIDEMWKVAQRELATGFYYSPPSENEQLFGARRKIPEYKFIAEVV
                     AFDKETMTATIRQRNVINEGDQVEFYGPGFRHFETTIQDLHDSNGEKIDRANKPMELL
                     TIKLDREIYPGDMIRACKSGLINLYQEDGQSLTIRA"
     gene            complement(1921..>2246)
                     /locus_tag="AC1PIW_001864"
     CDS             complement(1921..>2246)
                     /locus_tag="AC1PIW_001864"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606756.1"
                     /translation="MKRFLEVIFEQFTIFLEHGKAPKSIEPDSKEEIKNNLELALYKK
                     AAVHVIYGDRSFTGDIVKYDTDRQRIIMKNFKRQVTSIIKIEDIKKVTLVPDSIKTSQ
                     QQTKG"
BASE COUNT          635 a          492 c          502 g          617 t
ORIGIN      
        1 aacgcctttg ggcgattttc taatctctgc cctaggtttc cgagtgaaac aacatcgttt
       61 acagtattct atcctggcag agttagctct gtcacttaca atgtaaggtc agagcacgaa
      121 aacgcctttg agcgattttc taaactctgc actaggtttc cacatggaaa agtatcgttt
      181 catgtggaaa ccgtcgcaaa tagaggagat tttacatgat gtcaaaaaca ttaaagcgtc
      241 ccgaggtctt gtcacctgct gggactttgg agaaattgaa ggttgccatt gattatggtg
      301 ctgatgcggt ctttgttggc ggccaggcct acggtctgcg tagccgagct ggtaacttta
      361 ccatggacga gatgcgggaa gggattgaat atgcccatgc ccgtggtgcc aaggtctatg
      421 tagctgccaa catggtcacc cacgaaggta atgaagaagg agcaggagcc tggttccgcg
      481 aattgcgtga catgggcttg gatgccgtta tcgtatctga cccggctctc atcgttatct
      541 gtgcaaccga agcgccaggc cttgaaatcc acctgtcaac ccaggcttct tccaccaact
      601 acgaaacctt tgagttttgg aaggaattgg gcttgactcg tgtcgtcctg gcccgcgaag
      661 tgactatgga agaggtggct gaaatccgta aacggacaga cgttgaaatc gaagcctttg
      721 tccacggtgc catgtgtatc tcctactcag gtcgttgcgt tctctccaac cacatgagca
      781 aacgcgatgc caaccgcggc ggttgctccc aatcttgccg ttggaagtac aacctctacg
      841 acctaccttt cggcgaagaa cgccgttcga ttaaggggga aataccagag gagttctcta
      901 tgtctgcagt ggacatgtgt atgatcgacc atattccaga tatgattgaa aacggggtcg
      961 atagcctgaa aatcgaaggc cgtatgaaat ctgttcacta cgtatcaaca gtgaccaact
     1021 gctacaaggc tgcggtagat gcctatcttg aaagcccaga aaggtttgaa gcgatcaagc
     1081 aagacctgat tgatgagatg tggaaggttg cccaacgtga attggcaaca ggattctact
     1141 atagcccgcc gagtgaaaat gagcaactct ttggagcccg ccgcaaaatt ccagaataca
     1201 aattcatcgc cgaagtagtg gcttttgaca aggaaaccat gactgcaacc attcgtcagc
     1261 gtaatgttat caatgaaggt gaccaagtag aattttacgg accaggtttc cgtcattttg
     1321 aaacgactat tcaggacttg catgattcaa atggagaaaa gattgaccgc gccaacaagc
     1381 cgatggaact cttaactatt aagctggacc gtgaaatcta tcctggtgac atgattcgtg
     1441 cctgcaaatc aggcctcatc aacctctacc aagaagatgg gcaatctcta actattcgtg
     1501 cataaacata tagacattta gtttttcttt tattacttcg acgtaagagg aaactccgtt
     1561 tccttatttc caacttcaaa cactccactg gattgtttga actcgctttg ccgtactctg
     1621 caggggtgac ttgctccgca agtgctatct ccaacctcca aaggttcccc aaacctttgg
     1681 agcgagtact aaaagtaaac taaaagacta taaaaaggct gaaatttcag cctcagaacg
     1741 tagataaact cttaaaaata taaaaaaatg aagaagtttg cgaaagatag caagtcatta
     1801 aactcttaga aacattgata tttcggcgtt tctgagagtt tttaattttc aacataaaga
     1861 aaaaagattg aagaaaattg tccaaaatgg actttttctt caatctgcaa ggctgaaatt
     1921 tcagcctttt gtttgttgtt gagaagtctt gatgctatct ggaaccaagg tgactttttt
     1981 gatgtcctcg atttttataa tgctggtaac ctgacgtttg aaatttttca tgataatccg
     2041 ttggcgatcg gtatcatatt tcacaatgtc acctgtgaaa ctacgatcac catagatgac
     2101 atggactgca gcttttttat ataaagccag ctccaagtta ttcttgattt cttccttgct
     2161 atctggctca atagacttag gagctttgcc atgttctaaa aagatggtga actgctcgaa
     2221 aataacttct aaaaacctct tcatgg
//
LOCUS       DBNFCK010000175         2204 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.176, whole genome shotgun sequence.
ACCESSION   DBNFCK010000175 DBNFCK010000000
VERSION     DBNFCK010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.176"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(76..618)
                     /gene="hpt"
                     /locus_tag="AC1PIW_001865"
     CDS             complement(76..618)
                     /gene="hpt"
                     /locus_tag="AC1PIW_001865"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HIP6606757.1"
                     /translation="MLDKDIKKILVSEEEIVAKCKELGQILATDYADKNPILVGILKG
                     SIPFMAELMKHIDAHVETDYMVVSSYHGGTESCGTVKIIKDLDNSVAGRYIIFVEDII
                     DTGRTLKELKELFALRQAASIKIATLLDKPEGRVVEIEPDYTCFTIPNEFVVGFGLDY
                     DENYRNLPYVGVLKEEVYTK"
     gene            complement(626..1894)
                     /gene="tilS"
                     /locus_tag="AC1PIW_001866"
     CDS             complement(626..1894)
                     /gene="tilS"
                     /locus_tag="AC1PIW_001866"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIP6606758.1"
                     /translation="MKDRFLKVTLDGHFFDKHKKVLVAVSGGLDSMNLFHLLYECKQV
                     LGIELGIAHINHGQREESVIEEKYLRQLAEESNVPFYLSYFEGVFSEEAARKWRYGFF
                     ATIMEKEGYTALVTAHHADDQAETVFMRLIRGGRLRHLSAIQPTQPFATGELIRPLLS
                     FKKADFEKLFHFEDCSNADFKYFRNRVRNDYLPKLKQENPKIELTLNNLAVDTNNLFQ
                     ALRDLTQDLSVTDVMSFQQQTPAVQSYLLEEYLEKFPDLQLSRPQFNEVLHILRSKAN
                     YYHLLKNDYMLEKDYHRFQIYKIGPETDSQPEKIMIESEGIFSYGSYIFSLNHPLEEA
                     DSILYFPTENPIVVRRRQAGDTILINGVNKKLRRWFIDNKISQKVRQNALIIEQNGEI
                     YGITNLVSSDLSKSVKNDIIKATLYIKMKE"
     gene            complement(1895..>2204)
                     /locus_tag="AC1PIW_001867"
     CDS             complement(1895..>2204)
                     /locus_tag="AC1PIW_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIP6606759.1"
                     /translation="TGSEWSMVTRETSAEIAGKMMEALYIQNGYVLESLLSTQFDNQR
                     ISKDISVPVAHKIGDADDVKHDVAIVYAGSPFVLSIFTDKSNYDEITQIANDIYRILK
                     "
BASE COUNT          685 a          420 c          338 g          761 t
ORIGIN      
        1 gagaataatg agaaaaggat tttttataaa tcctttattt ggttgattgt tcataatata
       61 gtaggaaacc tttttctatt ttgtgtaaac ttcctctttg agtacaccaa cataaggaag
      121 attacgataa ttttcatcat aatctaagcc aaatccaact acaaattcat ttggaatagt
      181 aaagcaagtg tagtctggct cgatttcaac aacacgacct tctggtttat caagaagagt
      241 ggcaatttta atagaagctg cctgacgcaa agcaaagagt tctttcaatt cttttaatgt
      301 gcgaccagtg tcaataatat cctctacgaa gatgatatat cgacctgcaa cactattgtc
      361 caaatccttg ataatcttaa cggtaccaca actctccgtt ccgccatggt agctagaaac
      421 aaccatataa tccgtttcta catgggcatc aatatgtttc attaattctg ccataaatgg
      481 gatagaacct tttaaaattc ctacaagaat aggattctta tctgcatagt ctgttgctag
      541 aatttgaccg agttctttgc attttgcaac gatttcttct tctgaaacaa gaattttctt
      601 aatatctttg tccaacatag ttgttctact ctttcatttt tatatataag gtagctttga
      661 ttatatcatt ttttactgat ttactcaaat cactgctaac aagattcgta attccataaa
      721 tttctccatt ctgctcgata ataagcgcat tttgtcgaac tttctgtgag attttattat
      781 caataaacca acggcggagt tttttattta ctccgtttat taaaatggta tccccagcct
      841 gccttctcct cactacaatt ggattttctg taggaaaata taaaatacta tccgcttctt
      901 ccaaaggatg attgagagaa aaaatatatg aaccgtaaga aaaaatcccc tctgattcta
      961 tcatgatttt ttctggttga ctatccgtct ctggtcctat tttataaatt tgaaaacgat
     1021 gataatcctt ttctagcatg taatcatttt tcaataaatg atagtagtta gcttttgaac
     1081 gcaagatgtg taatacttca ttaaattgtg gacgagagag ctgtaaatca ggaaattttt
     1141 ccaaatattc ctctaataga taactttgta ccgcaggtgt ctgttgttga aaactcatca
     1201 catctgtaac ggataaatcc tgtgtcaaat ctcttagcgc ttgaaataga ttatttgtat
     1261 caactgcaag attattgaga gtaagttcga ttttaggatt ttcctgtttt aactttggta
     1321 aatagtcatt tctgactcta tttcgaaaat atttgaaatc tgcattgctg caatcttcaa
     1381 aatgaaatag cttttcaaaa tctgcctttt taaaagataa aagtggtcga ataagttccc
     1441 ctgttgcaaa aggctgagtc ggttgaattg ctgataagtg acgtaaacgc ccgcctctaa
     1501 tcaatcgcat aaagacagtt tctgcttgat catctgcatg gtgggctgtc actagtgcag
     1561 tgtagccttc cttctccatg attgtagcaa aaaatccata acgccatttt cgcgccgctt
     1621 cttctgaaaa aacaccttca aaataggaca gataaaaggg aacattactt tcttctgcta
     1681 actgtctaag atatttttcc tcaatgacag attcttctct ctgtccatga ttaatatgag
     1741 caattccaag ttcaatccct aacacttgtt tgcattcgta taaaagatgg aataggttca
     1801 tagaatctaa cccaccagat acggctacta gtactttctt atgtttatca aaaaagtgac
     1861 catccagtgt cacttttaaa aatctatctt tcatttactt taaaatcctg taaatatcat
     1921 ttgcaatttg tgtaatctca tcataatttg atttatctgt aaaaatagat aaaacaaagg
     1981 gagaccctgc gtaaacaatc gctacatcgt gtttgacatc gtctgcatcg ccaattttat
     2041 gagcaactgg aactgagatg tctttcgaaa tacgttgatt atcaaattga gtagataaca
     2101 aactttctag cacatagcca ttttgaatat ataaggcttc catcattttc cctgcaatct
     2161 cagctgaagt ttcacgtgta accatactcc actcactacc tgtt
//
LOCUS       DBNFCK010000176         2180 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.177, whole genome shotgun sequence.
ACCESSION   DBNFCK010000176 DBNFCK010000000
VERSION     DBNFCK010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.177"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..687
                     /locus_tag="AC1PIW_001868"
     CDS             <1..687
                     /locus_tag="AC1PIW_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6606760.1"
                     /translation="LNQIGSMIEEPSYYPNLTGYENLLVFQKMVGFDKNNIRPTLELV
                     GLADEKNRKKLVKAYSLGMKQRLALAFALVKKPKILLLDEPTNGLDPAGIHEIRELIV
                     QLAKEKGLTVFISSHILPEIEHIADRVGIINHGRLVYEGEIEAIKSNTWIEIGGDFSQ
                     KNIVQTLVELGTVEILSASESQVTLRDLDNDRLADLVSYLIENGYRIFRVVRESETLE
                     DIFLNLTKEA"
     gene            689..1435
                     /locus_tag="AC1PIW_001869"
     CDS             689..1435
                     /locus_tag="AC1PIW_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6606761.1"
                     /translation="MNGVYIEWLKSRRTKSISIVIILMLVATLWNIATFISAFSSHPE
                     LRMAGTLFSNQNVNLLMFPIAVCVFASRIVGNEHEGQTFKLQIANGQSLTAIFRDKFF
                     FMLVSFVIMSAIEIMIIYLFGTQAGISISLQIIGIQFIGQILAIFSLICLYLTLAMIL
                     EKQGILLALGLLGGFLGIILNPKSYGFTSLLNPITGSGSLAPYKYQFLGDGTITYNLD
                     SQLLEKIVCYAIYCLILYLLAVLILRKKGN"
     gene            1437..2171
                     /locus_tag="AC1PIW_001870"
     CDS             1437..2171
                     /locus_tag="AC1PIW_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6606762.1"
                     /translation="MKDYFLAEWTKTRKIQLLVIGIAFLSFSSMIGLGIYFANRDVLI
                     DGTQSLVLWGQLTFYNSTLLYPPMLAIIAGLLLVPEFERKTLDMLKANQVSMRKLYLG
                     KLMSSFLLILPIQLLLLLIFIVAAKIDGISFDLSLATHVKWIALSLLSSFPIITLQSY
                     ITVKTRNFSKGVGVATIGSMLNFVLIFINESFTKFFPYSQPMIGLRSRALQDMTFLEF
                     ILFITVNIVFSFIFYQLTIKNLENSK"
BASE COUNT          654 a          335 c          412 g          779 t
ORIGIN      
        1 ctaaatcaga ttggatctat gatagaagaa ccgtcctatt atcctaattt aactggatat
       61 gaaaatttat tagtgtttca aaaaatggtg ggatttgata aaaataatat ccggccaaca
      121 ttggagctcg ttggattagc tgatgaaaaa aatcgaaaaa aattagttaa ggcctattct
      181 cttggtatga aacagcgact tgccttagca tttgctttag tgaaaaagcc gaaaatctta
      241 ttattagatg agcccacaaa tggtcttgat ccagctggta ttcatgaaat tcgtgaatta
      301 attgttcaat tagccaaaga aaaaggtctg actgtcttta tttccagtca cattcttccg
      361 gaaattgaac acattgcaga tcgagttggg attatcaatc atggtcgtct tgtctatgag
      421 ggagaaattg aagctattaa gtcaaatact tggattgaaa ttggcggtga tttttcacag
      481 aaaaatattg ttcagacctt agttgaactt ggaacggtag aaattttgag tgcatccgaa
      541 agtcaagtaa ctttaaggga tttggataac gatcggttag cagatctggt gagctattta
      601 atagaaaatg gataccgaat attccgagtg gttagagaaa gtgaaactct tgaagatatt
      661 ttcctaaatc taacaaagga ggcgtaagat gaatggtgtt tacattgaat ggctaaaaag
      721 tagacgaaca aaatctattt caattgtcat tattttgatg ttggttgcca cactttggaa
      781 tattgcaact tttatctctg ctttctcttc acatccagag ttaagaatgg ctggaacctt
      841 gtttagtaat cagaatgtca atctacttat gttcccgatt gcagtttgtg tgtttgctag
      901 tcgaattgta gggaatgaac atgaagggca gacctttaaa ctacaaatag ctaatggtca
      961 aagcctaaca gccatttttc gtgataaatt cttttttatg ctggtttcct ttgttattat
     1021 gtctgctatc gaaattatga ttatttatct tttcggaaca caggcaggaa tctctatttc
     1081 tttgcagatt attggtattc agtttattgg gcagatactt gcaatatttt cattgatttg
     1141 tctttatctg accttggcca tgattcttga aaagcagggg attctgctag cactaggact
     1201 tttaggtggt tttctaggaa ttatcttaaa cccaaaaagt tatggtttta ccagtttact
     1261 taacccaatc actggttcag gaagtcttgc gccttataaa tatcaatttc tgggtgacgg
     1321 cacaattacc tataatttag atagtcagtt gcttgagaaa atcgtctgtt atgccattta
     1381 ctgcttgata ctctaccttt tggcggttct aatattaaga aagaaaggga actaagatga
     1441 aagattactt cttagctgag tggacaaaaa caaggaaaat tcaattatta gtcattggga
     1501 ttgccttttt gagtttctct agtatgatag gattgggtat ctattttgct aatcgtgatg
     1561 ttttaatcga cggaactcaa tctttggttc tgtggggaca actgaccttt tacaatagta
     1621 ccttacttta tcctcctatg ttagccatta ttgcaggtct cttattagtt cctgaatttg
     1681 agcgaaaaac tcttgacatg ctaaaggcta atcaagtttc tatgaggaaa ttatatttag
     1741 gaaaattaat gagcagtttt cttcttattc ttccaattca actcttattg ctgctgattt
     1801 ttatagttgc ggcaaaaatt gacggcattt catttgacct tagtttagcc acacatgtta
     1861 aatggattgc tttatcgctt ttgtcttcct ttccaattat tacactccaa tcctatatca
     1921 ctgtaaaaac tcgaaatttc agtaagggag tcggtgttgc taccattggt agtatgctta
     1981 attttgtatt aatttttatc aatgaaagtt ttacgaaatt tttcccttac tctcaaccga
     2041 tgattggatt acggagtagg gcattgcaag atatgacatt tttagagttt atcttattta
     2101 ttacagtgaa tattgtgttt agttttattt tttatcagtt aactatcaag aatttggaaa
     2161 atagtaaata aaaacctttt
//
LOCUS       DBNFCK010000177         2166 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.178, whole genome shotgun sequence.
ACCESSION   DBNFCK010000177 DBNFCK010000000
VERSION     DBNFCK010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.178"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            81..1229
                     /gene="cps2T"
                     /locus_tag="AC1PIW_001871"
                     /gene_synonym="wchF"
     CDS             81..1229
                     /gene="cps2T"
                     /locus_tag="AC1PIW_001871"
                     /gene_synonym="wchF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta 1-4 rhamnosyltransferase Cps2T"
                     /protein_id="HIP6606763.1"
                     /translation="MKHVFIIGSRGLPAKYGGFETFVQQLVSHQQSEKICYHVACLSD
                     TSHHQHSTYLGADCFTINPPKLGPARVIAYDMMAITYALKIIKEQQIERPIFYILGNT
                     IGGFIVPFAKQIHSVGGTLFVNPDGLEWKRAKWAKPVQRYLKFSEKMMVKYADLIIAD
                     NEGIEDYIQSEYSATNTRFIAYGTDLSPTKLTKESEQVRQYFTNWNIKEKEFYLIIGR
                     FVPENNYVTAIREFMASNTRRDLVIVANHEGSAYFQKLKDETGFISDKRIKFVGTVYD
                     QELLKYLRQECRAYIHGHEVGGTNPSLLEALAQTNENLVLGVDFNRKVALEGARYWSK
                     ESGSLSQLINQIDENAEAMELGEIAKKHMKDEYTWEKIVQEYEELFLQ"
     gene            1226..2155
                     /locus_tag="AC1PIW_001872"
     CDS             1226..2155
                     /locus_tag="AC1PIW_001872"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002933134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIP6606764.1"
                     /translation="MKVNILMSTYNGQQFLAEQIRSIQEQSYIDWTLFIRDDGSSDRT
                     REIIKDFVEQDQRIHFIDVETDENLGVIKSFHRLVHYDTADFYFFSDQDDVWLPNKLE
                     VSLKKAQAYPTNLPLMVYMDLKVVNQNLEVMAESMVKSQSHHANTELVQELTENTVTG
                     GVAMINHHLAEMWQVTEDILMHDWYLALLASALGKLVFIDKPGELYRQHSDNVLGART
                     LSKRFKKWIRPHILFAVYWDLIKNSQKQARHLLQMPLSQSNRELIEAFVTIMDKPMLE
                     RFRILRKYGLRKNKTFHTLVFTTLIVTKFAYKE"
BASE COUNT          720 a          365 c          449 g          632 t
ORIGIN      
        1 taaccaaact aggatagaat taggaaaaaa aactaaaaaa tggtataatg aaaagaactg
       61 caatttgatg gagaggattc atgaaacacg tttttattat cggtagtcgt ggactgccag
      121 caaaatatgg tggctttgag acctttgtcc aacaattggt cagtcaccag caatctgaga
      181 agatttgtta tcatgtggct tgtttatcgg atacaagtca tcaccaacat tcgacctacc
      241 tgggtgcaga ttgcttcaca attaatccac caaaacttgg accggctcgt gtgattgctt
      301 atgatatgat ggcgatcact tatgccctta aaatcattaa ggaacagcag atagagcgac
      361 caattttcta tatcttggga aataccatag gtggcttcat agtacctttt gcgaaacaga
      421 ttcactctgt tggaggtacc ttgtttgtca atccagatgg tttggaatgg aaacgggcca
      481 agtgggctaa gccagtccaa aggtatctaa aattttctga aaaaatgatg gtcaaatatg
      541 ctgatttgat tattgctgat aatgagggaa ttgaggatta tattcaatct gaatactcgg
      601 ctaccaatac acgctttatt gcctatggta cagatttaag tccgactaag ttaacaaaag
      661 aatctgagca agttcgccaa tattttacaa attggaacat taaagagaaa gaattttatc
      721 tgattattgg tcgttttgta cctgagaata actatgtgac agcaattagg gagtttatgg
      781 caagtaacac gagacgtgat ttagtcatcg ttgcaaacca tgaaggctct gcctattttc
      841 aaaaattgaa agatgagaca ggctttatat ccgataagcg gattaagttc gtaggaacag
      901 tatatgacca agaactactt aagtatctgc gccaagaatg tcgagcttat attcatgggc
      961 atgaagttgg aggaaccaat cctagtctcc tagaagcgct ggcgcaaacc aatgaaaatt
     1021 tggttttagg tgttgatttt aaccgcaaag ttgccctaga aggtgctcgg tattggagta
     1081 aggagtcagg tagcctgagt caattaatta accaaattga tgagaatgct gaagcgatgg
     1141 aattaggaga aattgcgaaa aaacacatga aggatgaata cacctgggaa aaaatcgtcc
     1201 aagagtacga ggaattattt ttacaatgaa agtaaatata ctcatgtcga cctacaatgg
     1261 tcaacagttc ttagctgagc aaattcgctc tatccaagaa cagtcataca tagactggac
     1321 actattcatt cgtgatgatg gttccagtga tcgaactaga gagattatta aagattttgt
     1381 tgaacaagat cagcgaattc acttcataga tgttgagaca gacgaaaatc tcggtgtcat
     1441 caaaagtttt cataggctag ttcattatga tactgctgat ttctattttt tcagtgacca
     1501 agatgatgtt tggctaccaa ataaactgga agttagcctg aaaaaagcac aagcctatcc
     1561 aacaaatttg ccattgatgg tttatatgga cctcaaagtt gtcaatcaaa atctagaagt
     1621 tatggcagaa agcatggtaa aatctcaatc tcatcatgcc aatacggaat tggtgcaaga
     1681 gttgactgag aatactgtaa ctggcggtgt cgccatgatt aatcatcatc ttgctgaaat
     1741 gtggcaagta actgaggata ttttgatgca tgactggtat ttagctttgt tggcctcggc
     1801 tttgggtaaa ctggttttta ttgacaagcc tggcgaactc tatcgccagc attctgataa
     1861 tgttttaggg gctcgaacac tctcaaaacg ctttaaaaaa tggattcgcc ctcatatttt
     1921 atttgcagtt tattgggact tgattaaaaa tagtcaaaaa caagcccggc atttattaca
     1981 aatgcccctt tctcaatcaa atagagaatt aatagaagcc tttgtaacca ttatggataa
     2041 accaatgctt gaacgattca ggattttgag aaaatatggt ttaagaaaaa acaaaacctt
     2101 ccatacatta gtattcacaa cactgattgt gacaaaattt gcatataagg aatagttatg
     2161 aattta
//
LOCUS       DBNFCK010000178         2160 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.179, whole genome shotgun sequence.
ACCESSION   DBNFCK010000178 DBNFCK010000000
VERSION     DBNFCK010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.179"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            104..2056
                     /gene="gyrB"
                     /locus_tag="AC1PIW_001873"
     CDS             104..2056
                     /gene="gyrB"
                     /locus_tag="AC1PIW_001873"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HIP6606765.1"
                     /translation="MTEEIKDLQEKAQEYDASQIQVLEGLEAVRMRPGMYIGSTSKEG
                     LHHLVWEIVDNSIDEALAGFADKIEVYIEPDNSITVIDNGRGIPVDIQEKTGRPAVET
                     VFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVYKNGQVYFQEYKRGEVV
                     ADLEVVGETDRSGTTVHFTPDPEIFTETTEFDFAKLNKRIQELAFLNRGLNLSITDKR
                     EGVEQTKEYHYEGGIASYVEYLNENKDVIFETPIYTEGEMDDITVEVAMQYTTSYHEN
                     VVSFANNIHTHEGGTHEQGFRTALTRVINDYAKKNKILKENEDNLTGEDVREGLTAVI
                     SVKHPGPQFEGQTKTKLGNSEVVKITNRLFSDAFAEFLLENPQIARRIVEKGILASKA
                     RIAAKRAREVTRKKSGLEISNLPGKLADCSSNDAAKTELFIVEGDSAGGSAKSGRDRE
                     FQAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGADFDVSKARYQKLVIMTD
                     ADVDGAHIRTLLLTLFYRYMRPVVEAGYVYIAQPPIYGIKVGSEIKEYIQPGVNQEQE
                     LQDALERHSTGRSKPTIQRYKGLGEMDDHQLWETTMNPENRLMARVSVDDAAEADKIF
                     DMLMGDKVEPRREFIEENAVYSTLDV"
BASE COUNT          671 a          378 c          514 g          597 t
ORIGIN      
        1 attcgttaga atgatttttg ctctaaaaat atgtttcgct tttatccaga tggattggct
       61 tttctatttt taaggagcaa caaatagaaa ataaggaaaa gaaatgacag aagaaatcaa
      121 agatttacaa gagaaggctc aagaatatga tgccagtcag attcaagtct tggaaggtct
      181 tgaagctgtt cgaatgcgtc cagggatgta tatcggctca acatctaaag aaggtctcca
      241 tcacttggtt tgggaaattg tcgacaactc aatcgatgag gcgctggctg gttttgcgga
      301 taaaattgaa gtttatattg agccagataa ctccatcacg gttattgata atggacgtgg
      361 gattccggta gatattcagg aaaaaactgg tcgtccagcg gtagaaactg tctttacagt
      421 gctccatgca ggtggtaaat ttggcggagg cggttataag gtctcaggtg gattgcacgg
      481 cgtaggttca tcagtcgtaa acgccctatc tactcagcta gacgtccatg tctacaaaaa
      541 tggtcaggta tatttccaag agtataaacg aggtgaagtc gttgcagatt tggaggttgt
      601 tggagaaaca gaccgttctg gtacaactgt acattttaca cctgatccag aaatttttac
      661 tgagacgact gagttcgact ttgcaaaatt aaataagcgg atccaagaat tggccttcct
      721 aaatcgtggt ttaaatctct ctattacgga caaacgtgag ggagtagagc aaacaaagga
      781 atatcactat gaaggtggta ttgcttccta cgttgagtat cttaatgaga acaaggatgt
      841 catttttgag acccctatct atacagaagg tgaaatggat gatattaccg ttgaggttgc
      901 catgcaatat acgacgagtt atcatgaaaa cgtagttagt tttgccaaca atattcacac
      961 acatgaaggt ggtacccatg agcaaggatt ccgtacagct ctgacacgtg ttatcaacga
     1021 ctacgccaag aaaaataaaa ttctcaaaga aaatgaggat aatttgactg gtgaagatgt
     1081 gcgtgaaggt ttgacagcgg tcatttctgt taagcaccca ggtcctcaat ttgagggaca
     1141 aactaagaca aaacttggga atagtgaagt ggtcaaaatt accaaccgcc ttttctctga
     1201 tgcctttgca gaatttcttt tggaaaatcc acagattgct agacggattg ttgaaaaagg
     1261 tattttggca agcaaggctc gtattgctgc taagcgtgcg cgtgaggtaa ctcgtaagaa
     1321 atcgggtctt gagatttcaa acctcccagg taagttagcg gattgttcct caaatgatgc
     1381 agcgaagaca gagcttttca tcgttgaggg ggactcagcc ggtggttcag ctaaatccgg
     1441 tcgcgaccgc gaatttcagg ctattttacc gattcgtggt aagattttga acgtagaaaa
     1501 agccagcatg gacaagattt tggccaacga agaaatccgt agtctattta cagctatggg
     1561 aactggcttt ggtgctgatt tcgacgttag caaggcacgt tatcaaaaat tagtcattat
     1621 gacagatgcc gatgtcgatg gtgcccatat tcggacactc cttctgacat tattttaccg
     1681 ctacatgcgt cctgttgtag aagcgggata cgtatatatc gctcaaccac ctatttacgg
     1741 tatcaaggtt ggaagtgaga taaaagaata tattcaacct ggggttaatc aagagcaaga
     1801 attgcaagat gccttagaaa gacatagtac gggacggtcc aagccaacga ttcaacgtta
     1861 caaaggtttg ggagaaatgg atgatcatca gttgtgggaa actacgatga atcctgaaaa
     1921 tcgtctcatg gcacgcgttt cagttgatga tgcggcagaa gccgataaga tttttgacat
     1981 gttaatgggg gataaggttg aacctcgtcg agagtttatc gaggaaaatg ctgtttattc
     2041 aacattggat gtctaaaaac taaaaaaaca agtgcatata ctagagattt atggtataat
     2101 taagcgatat tttctagtaa ttactatgag ctaaggagat ttacatgcct acaggaacaa
//
LOCUS       DBNFCK010000179         2096 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.180, whole genome shotgun sequence.
ACCESSION   DBNFCK010000179 DBNFCK010000000
VERSION     DBNFCK010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.180"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            1..651
                     /locus_tag="AC1PIW_001874"
     CDS             1..651
                     /locus_tag="AC1PIW_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIP6606766.1"
                     /translation="MSRILVVEDDIVISQVVCEFLKEHGYQVESVFDGKVALERFQEE
                     QFDLIVLDIMIPSMTGLEVLKEIRKTSQIPILMLTAMGDEYTQLISFNQIISDYVVKP
                     FSPTILVKRIENILRGKGETDSIEIGTILIQPTSGAVYMEEEEVQLTKKEYEVLLYLA
                     KRRGKIVSRDNLMMGIWGYTELDSRVLDNHIKNIRKKLPSLPLKTIVGRGYQIEGT"
     gene            653..2041
                     /locus_tag="AC1PIW_001875"
     CDS             653..2041
                     /locus_tag="AC1PIW_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIP6606767.1"
                     /translation="MGIARKNFIIVAAMISFISVTLLGLLYYAMPIYYNQVKKQELRH
                     DYMTVAKQLDGMPEAKIISEIDDFDIKTPNIIISLFSSDRKIIYPDPNDEMAKKHENE
                     YLENGDFDEIGSWGAEITSAEGVTYYLLFNYGFHSLSDVSQTLVTFYPFILLLIIVLA
                     VSVAFVYSRLSTRRIARISETTRRMQSLESGISCAVAGTDEITILAQDINSLYSKLLS
                     SIDELRTENERAMAREKEKSDFLRITSHELKTPIASMLGLVEGMIYNVGPFKDHDTYL
                     KKCKEILQEQSELVHSILEATNLDMALKESREQIRLDQLIDSSLTSYQSLAEVKGYQF
                     EVELSPVLIEANPVYFLKAIKNILDNAFRYSCKEAIIRVVLRQNQLIIENQVERVLSD
                     EELDQVFRPFYRPDYSRDKKDGGIGIGLFIVQKILEKHGFPYTFYASDTQTMRFMIHL
                     QSQSTANSNELI"
BASE COUNT          696 a          280 c          442 g          678 t
ORIGIN      
        1 atgtcaagga ttttggttgt agaggatgac atagttatta gtcaagttgt ttgtgagttt
       61 ttaaaagaac atggttatca ggtagaatct gtttttgatg gaaaggttgc tttagaaagg
      121 tttcaagaag aacaattcga tttaattgtt ttagatatca tgattccatc tatgacaggt
      181 ttggaagtac tgaaggaaat tcgcaaaact tctcagattc caattttgat gctgacagcc
      241 atgggtgacg aatatacaca gcttattagt tttaatcaga ttataagtga ttatgttgtt
      301 aaaccatttt caccaactat attggtaaaa cggattgaga atattttgag aggaaaagga
      361 gagacagata gcattgagat aggaacaatt cttattcaac caactagcgg ggcagtttat
      421 atggaagaag aagaagttca attgacgaaa aaagaatatg aagttttact atatttagct
      481 aaacgacgtg ggaaaattgt tagtcgtgat aacttgatga tgggaatatg gggatatacg
      541 gaattggata gtcgtgtctt agataatcat atcaagaata tccgtaagaa attgccgtca
      601 cttcctttga agacaatagt tggtcgtggt tatcaaatag agggtactta atatggggat
      661 tgccagaaaa aattttatta ttgttgctgc gatgatttct ttcatctctg taacattgtt
      721 agggttattg tattatgcta tgcctatata ttataatcag gttaagaaac aggaattgag
      781 acacgattat atgactgtgg ctaaacaatt ggatgggatg ccagaagcta agattatatc
      841 tgagattgat gatttcgaca taaaaactcc gaatattatt attagtcttt tttcgagtga
      901 tagaaaaatt atttatccag accccaatga tgaaatggct aaaaagcatg agaacgagta
      961 tttagaaaat ggggattttg atgaaattgg ttcttgggga gccgagataa catctgcaga
     1021 aggagttaca tattatctgt tatttaatta tggatttcac tcgttatctg atgtcagtca
     1081 aacattggta actttttatc cattcatatt gctgttgatt attgttctgg cagtttcggt
     1141 agcttttgtt tacagtagac tgtccactcg tagaatagca agaatttcag aaacaacaag
     1201 acgaatgcaa tcgttagagt ctggaatcag ttgtgctgtt gcaggaactg atgaaataac
     1261 aatattggct caggatatta acagcctgta tagcaagtta ttgtcaagta tcgatgaatt
     1321 aagaacggaa aatgaacgtg ctatggcacg tgagaaggag aagtctgatt tcctccgtat
     1381 cacatcacac gaattaaaaa ctccaatagc tagtatgttg gggttagtcg aaggcatgat
     1441 ttataatgtg gggccattta aggaccatga tacatattta aaaaaatgca aggaaatctt
     1501 gcaggaacaa tctgagttag ttcattctat tttagaagcg acaaatcttg atatggcttt
     1561 aaaggaaagc agagaacaaa tccgattaga tcagttgatt gattcctctt taacaagtta
     1621 tcagagtttg gcagaggtta agggctatca atttgaagta gaactatcac cggttctcat
     1681 tgaggcaaat cctgtttatt tcttaaaagc tattaagaat attttagata atgcctttcg
     1741 gtactcatgt aaagaggcta ttattcgtgt agtactacgc caaaatcaat taataatcga
     1801 aaatcaggtt gaacgtgttt taagtgacga ggaattggat caagtctttc gtccattcta
     1861 tcgtccagac tacagtcgtg ataagaaaga tggtggcata gggattggtt tgtttattgt
     1921 acagaaaata ttagaaaaac acggctttcc gtacactttt tatgcttctg atacacaaac
     1981 tatgcgtttt atgattcatt tgcaatcaca aagtacagcg aatagtaatg aattgatata
     2041 ggtaaaatat agacaatatt tagtttttct tttattactt cgacgtaaga ggaaac
//
LOCUS       DBNFCK010000180         2006 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.181, whole genome shotgun sequence.
ACCESSION   DBNFCK010000180 DBNFCK010000000
VERSION     DBNFCK010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.181"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            243..1787
                     /locus_tag="AC1PIW_001876"
     CDS             243..1787
                     /locus_tag="AC1PIW_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HIP6606768.1"
                     /translation="MSLQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGNFAKSDWMDIEKQRGISVTSSVMQFDYAGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRNIPVFTFINKLDRDGREPLDLLQELEEVLG
                     IASYPMNWPIGMGKSFEGLYDLHNKRLELYRGDERFASLDEGDKLFGSNPFYAQVLDD
                     IELLAEAGNEFSEQAILDGDLTPVFFGSALTNFGVQTFLDTFLEFAPEPHGHKTTTGD
                     VIDPLNKDFSGFVFKIQANMDPRHRDRIAFVRVVSGEFERGMAVNLPRTGKGAKLSNV
                     TQFMAESRENVENAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAIQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMAPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
BASE COUNT          572 a          419 c          487 g          528 t
ORIGIN      
        1 gttcattatt ttatctttct ttttctagta tatagaatac atctcatact tattgtaaca
       61 tatttataat acaatagtat aggtctgtag acctattctt caatagtttg aaaaattccc
      121 ttatttccga tataataaga gagttgtcta gtaaagaata aggatgaatc caatgtaaat
      181 cgaataggat aagagctcag ctctgctatg aatcaaaata caaaaaatga aaaggaaaca
      241 ttatgtcact acaagaagaa atcaagaaac gccgcacctt tgcgattatc tctcacccgg
      301 acgcgggtaa aaccaccatt accgagcagt tgctctactt tgggggtgag attcgtgaag
      361 caggtacggt caaggggaaa aagactggta actttgccaa atctgactgg atggatattg
      421 agaagcaacg tggtatctct gttacctcat ctgtcatgca gtttgactat gcaggcaagc
      481 gggtcaatat cctagataca ccagggcacg aggacttctc agaagacact tatcggacct
      541 tgatggcggt ggatgcggct gtcatggtgg tggactctgc caagggtatc gaggcccaga
      601 ccaaaaagct ctttgaggtt gttaagcacc gcaacattcc tgttttcact tttatcaaca
      661 agttggaccg tgacggtcgt gagccacttg atttgttgca ggagttggaa gaagtcttgg
      721 gcattgccag ctatccaatg aactggccga ttggtatggg gaaatccttc gagggactct
      781 atgacctcca taacaaacgt ttagaactct atcgaggtga tgagcgtttt gcaagcttgg
      841 acgagggtga caagctcttt ggttctaacc ccttttatgc acaagttctg gatgatattg
      901 aacttctggc ggaagctggg aatgaatttt cagaacaggc gattttggac ggcgatttga
      961 cacctgtttt cttcggctca gccctgacaa actttggtgt gcagaccttc cttgacacct
     1021 tcttggaatt tgctccagag ccacatggtc acaagacaac gacaggggat gtcattgacc
     1081 cgctcaataa agatttttca ggctttgtct tcaaaatcca agccaacatg gaccctcgtc
     1141 accgcgaccg tatcgccttt gtccgtgtgg tttcgggcga atttgaacgc ggtatggcgg
     1201 ttaacctgcc tcgtacaggt aagggagcca agttgtccaa cgtgacgcag ttcatggccg
     1261 agtcccgtga gaatgtggaa aatgcagtag cgggtgacat tatcggggtt tacgatacag
     1321 ggacttatca ggtaggagat accttgaccg taggcaagaa caagtttgaa ttcgagccgc
     1381 taccgacctt cacccctgaa attttcatga aagtttctgc caaaaatgtc atgaaacaaa
     1441 aatccttcca caagggaatt gagcaactgg tgcaagaagg agctatccag ctctacaaga
     1501 actaccagac aggcgagtat atgctgggtg ccgttggtca gctccagttt gaagttttca
     1561 aacaccgcat ggaaggcgag tacaatgccg aagtagtcat ggctccaatg ggtaaaaaga
     1621 cagtccgctg gatcaagcca gaagacctgg atgaacgcat gtcatcaagt cgtaacattc
     1681 tagctaaaga ccgctttgac caaccagttt tcctttttga aaacgacttc gccctccgct
     1741 ggttcgcgga caagtatccg gatgtggagt tggaagagaa gatgtagcgg aaagacagaa
     1801 atcagtaatc aacaaagttg atttgatttc gtcgtctttc cctcgcaagg gcggcgtcca
     1861 aagaactcga gcaatgcgaa gagtgatttg ttgtgacgcc tactgcgtcg aggttcagtg
     1921 aactttttct agtggtagat gagagctgat ggtaatcagc ttaccttgat aaagataact
     1981 ataaaaggat atgatagaat acatga
//
LOCUS       DBNFCK010000181         1986 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.182, whole genome shotgun sequence.
ACCESSION   DBNFCK010000181 DBNFCK010000000
VERSION     DBNFCK010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.182"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(5..754)
                     /locus_tag="AC1PIW_001877"
     CDS             complement(5..754)
                     /locus_tag="AC1PIW_001877"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIP6606769.1"
                     /translation="MKSYKNYIFDFYGTLVDIRTDENKLEVWNQLTQIYNAFGCSYRP
                     RQLKNAYHRFVEEAESSLAETVSYQYVEIDLETIFIRLLTDAPNENQSTNKPTDLETF
                     GQVVATIFRVLSREKLEAYENTLTSLQTLKDREVRLFILSNAQRIFTQAEIEQTGCAD
                     LMEKIYISSDFKMKKPEPAFLQLVLEENNLDVEETVMVGNDLTSDIAIAHELGMDSIL
                     LNTFPYSQEEIDSYRRLGWNFEVVGDIAELL"
     gene            complement(879..1244)
                     /gene="rplL"
                     /locus_tag="AC1PIW_001878"
     CDS             complement(879..1244)
                     /gene="rplL"
                     /locus_tag="AC1PIW_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HIP6606770.1"
                     /translation="MALNIENIIAEIKEATILELNDLVKAIEEEFGVTAAAPVAVAAA
                     GGAAEEAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPAMVKEGVATA
                     EAEEIKAKLEEAGASVTLK"
     gene            complement(1310..1804)
                     /gene="rplJ"
                     /locus_tag="AC1PIW_001879"
     CDS             complement(1310..1804)
                     /gene="rplJ"
                     /locus_tag="AC1PIW_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HIP6606771.1"
                     /translation="MSEAIIAKKAELVNAVAEQMKAATSIVVVDARGLTVEQDTVLRR
                     NLRGAEVEYKVIKNSILRRAAEQAGLEGLAELFVGPSAVAFSNDAVAPAKTIYDFAKT
                     ADALEIKGGAVEGKVSSKEEIEALATLPNREGMLSMLLSVLQAPVRNVAYAVKAVAEK
                     EDAA"
     misc_feature    complement(1831..1965)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
BASE COUNT          580 a          444 c          330 g          632 t
ORIGIN      
        1 ttatttacaa tagctccgct atatctccaa caacctcaaa attccaacca agtctcctat
       61 aagagtcgat ttcttcctga ctatacggga aagtattcag caaaatggaa tccattccca
      121 attcatgagc aatagctata tcggatgtca aatcattacc aaccataacc gtttcctcaa
      181 catcaagatt attttcctct aaaaccagtt gaagaaatgc tggttcaggc ttcttcatct
      241 taaagtcaga agagatataa attttttcca tcaaatcagc acaacctgtc tgttcaattt
      301 ctgcctgagt aaaaattcgt tgagcattgg agagaataaa taatcgcact tctctgtcct
      361 ttaatgtctg tagactagtt agggtatttt cataagcctc taacttttca cgtgaaagca
      421 cgcggaaaat cgtggctact acttgtccaa aagtctctaa atcagtcggc ttattggtac
      481 tttggttttc atttggtgca tccgttaata gacgaatgaa gattgtttct aaatcaattt
      541 cgacatactg ataactcact gtttccgcga gagagctctc tgcctcttct acaaaacgat
      601 gataggcatt tttcaactgg cgcggtcgat aggaacaacc aaaagcattg taaatctgtg
      661 ttagctgatt ccagacctct agtttattct catctgtacg aatatcaacc aatgtaccat
      721 aaaaatcgaa gatatagttt ttgtaacttt tcatgtcttt ctcctaatct ttagacatca
      781 ttttttatat tataccccac aacgaaaacg tttgcaacaa tagaacgaaa aaacctacca
      841 cattaagtga taggttttta ttttgattgg aaactctatt atttaagagt aactgaagcg
      901 cctgcttctt ccaatttagc tttgatttct tcagcttctg cagttgcaac gccttcttta
      961 accatagctg gtgcaccgtc aacaagctct ttagcttctt taagaccaag accagtgatt
     1021 tcacgtacaa ctttgataac gccaactttt ttgtcgccag cagatgtcaa ttcaacgtcg
     1081 aatgaatctt tagcttcttc agcagcacca ccagctgcag caacagctac aggagcagcc
     1141 gcagttacac caaattcttc ttcgatagct ttaacaaggt cgttaagctc aaggatagta
     1201 gcttctttaa tttcagcaat aatgttttca atgttcaatg ccattgtgat tttcctccaa
     1261 tttaggtttt taaatagttt gtagcactaa gcagctacgc tctgacgcat taagcagcgt
     1321 cttctttttc tgcaacagct ttgacagcgt atgcaacgtt gcgaactggc gcttgaagta
     1381 cagaaagcaa catagaaagc atgccttctc ggtttggcaa tgtagcaagt gcttcgattt
     1441 cttctttaga agaaacttta ccttctacag caccaccttt gatttcaaga gcatcagcag
     1501 ttttcgcgaa atcgtaaatt gttttagctg gagcaactgc atcgtttgag aatgcaactg
     1561 cagatggacc tacaaacaat tctgcaagac cttcaagacc agcttgctca gcagcacgac
     1621 gcaagattga gtttttgata actttgtact caacttctgc accacgcaag ttacgacgaa
     1681 gaactgtatc ttgttctact gtcaaaccac gagcgtctac aacaacgata gatgttgcag
     1741 ctttcatttg ctcagcaacg gcatttacta attccgcttt tttagcgata atagcttcac
     1801 tcattttttt acctccgttt ttattttggg ctaggcacaa aaaaatcgca cctaaaccta
     1861 gacacgaaag tacaaatacg tttatctcaa taatcgtttt gtgcctcggt aggatgttta
     1921 tgagtccagc tcccctactg tcttaggtca gttttttcaa acctcaatta tcataacaca
     1981 gtattt
//
LOCUS       DBNFCK010000182         1985 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.183, whole genome shotgun sequence.
ACCESSION   DBNFCK010000182 DBNFCK010000000
VERSION     DBNFCK010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.183"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..747
                     /locus_tag="AC1PIW_001880"
     CDS             <1..747
                     /locus_tag="AC1PIW_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6606772.1"
                     /translation="KLRYQGSLIGHLWSILKPMLLFTIMYLVFVRFLRFDDGTPHYAV
                     GLLLGMVTWNFFTEATNMGMMSIVSRGDLLRKLNFSKEIIVFSSVAGAAINYAINLMV
                     VFVFAAINGVPFTWSVLVIIPLFFELALLATGIAFILSSLFVRFRDIGPIWEVFLQAG
                     LYATPIIYSLTFILQRGQVTFAKLMMLNPVAQIIQDLRHFIVYSGNTTVSELIENPVI
                     VAIPYILPVVVYLLGITVFRKNAKKFAEIL"
     gene            747..1961
                     /locus_tag="AC1PIW_001881"
     CDS             747..1961
                     /locus_tag="AC1PIW_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6606773.1"
                     /translation="MVKNNNIAVKVEHVSKSFKLPTESSQSLRTTLVNLFKGIKGYVE
                     YNVLQDISFEVEKGDFFGIVGRNGSGKSTLLKILSQIYVPERGTVAVDGKLVSFIELG
                     VGFNPELTGKENVYLNGAMLGFTAEEIDAMYDDIVEFAELSEFMNQKLKNYSSGMQVR
                     LAFSVAIKAQGDILILDEVLAVGDEAFQRKCNDYFLERKKSGKTTILVTHDMGAVKKY
                     CNKALLIEKGYVKALGEPDDVANQYSFDNVLASISEVTENEEPLHQSDIVKDLQINLV
                     SKNQIEPDESIEIEFRYTVLEDIETHVAFTFLDLERHFDVYNDNSMDLKTFGKGEKFF
                     RVKCKLPSINQAKLKMAVSVRNSNKQPLLFAKTSDTPAIFISRKFSTDNIAEEDAATG
                     FIQRNSQWELLD"
BASE COUNT          618 a          268 c          424 g          675 t
ORIGIN      
        1 aaattacgtt accaaggttc tctcattggg catctctggt cgattttgaa accgatgtta
       61 ctttttacaa ttatgtattt agtatttgtt cgttttttgc gctttgatga tggaactcct
      121 cattatgcag tagggctact attgggtatg gtaacatgga acttttttac agaagcaact
      181 aatatgggga tgatgtctat tgtttcacgc ggagatttgt tgcgtaaatt gaatttttcg
      241 aaagaaatta ttgtcttttc ttcagttgca ggagctgcaa ttaattacgc cattaactta
      301 atggttgtgt ttgtatttgc tgctattaac ggggttccct ttacttggtc cgttttagtt
      361 ataatcccat tgttttttga attggcgtta ctagcaacag gtatagcttt cattctatct
      421 tccttatttg taagatttag agatattggt ccaatttggg aagtgttctt acaggctgga
      481 ttgtatgcga caccaattat ttattcattg acatttattt tacagcgtgg gcaagtaaca
      541 tttgctaagt tgatgatgtt gaaccctgtt gcacaaatta ttcaagactt gagacatttt
      601 attgtttatt ctgggaatac tacagttagt gaattgatag aaaatcctgt tattgtggca
      661 attccgtata ttcttccagt cgttgtttac ctattgggta taactgtgtt tagaaaaaat
      721 gccaaaaagt ttgcggagat tttgtaatgg taaaaaataa taatattgct gtaaaagtag
      781 aacatgttag caagagtttt aagcttccaa cggagagtag tcagagtcta agaactactt
      841 tagttaattt atttaaaggg attaaaggct acgtagagta taatgttttg caggatatca
      901 gttttgaagt tgaaaaaggg gacttttttg gaattgttgg tcgcaatggt tctggtaagt
      961 cgacactgtt aaaaatttta tctcaaatat atgttcctga aagagggact gtagctgtgg
     1021 atggtaaatt agtttctttt attgaacttg gtgttgggtt taatccggaa ctaactggta
     1081 aggaaaatgt atacctaaac ggtgcgatgt taggatttac tgcagaagaa attgatgcaa
     1141 tgtatgatga tattgttgaa tttgcggaat tgagtgaatt catgaatcaa aagttgaaaa
     1201 actattctag tggtatgcag gtacgtttag ccttttcagt tgccattaaa gctcaaggag
     1261 atattctgat acttgatgag gttctagcag tcggtgatga ggcctttcag cgtaaatgta
     1321 atgattactt cctagagcgt aaaaaaagtg gaaaaacaac cattcttgtc acccatgata
     1381 tgggagctgt gaaaaagtat tgtaataaag ctcttcttat agagaaagga tacgtcaagg
     1441 cattaggaga acctgatgat gtagctaatc aatacagttt tgataatgtg cttgcctcaa
     1501 taagcgaagt tacagaaaac gaagaacctc tgcatcagag tgatattgta aaagatttgc
     1561 agattaacct ggtctcaaaa aatcaaattg aaccagatga atcaattgaa atcgagttta
     1621 gatatacggt tttagaagat attgaaacac atgttgcatt tacattttta gatttagaac
     1681 gtcattttga tgtatataac gacaattcta tggatttaaa aacatttggt aaaggcgaga
     1741 agtttttccg agtgaaatgt aagctacctt caattaatca ggcaaaatta aaaatggcgg
     1801 tgtcagtacg aaatagtaat aagcagccgt tattatttgc caagacatct gacacaccgg
     1861 ctatttttat tagtaggaag tttagtactg ataatattgc agaggaagac gcagcaaccg
     1921 ggtttattca gagaaatagc caatgggagc tgctagatta ataaaatata acggaggcag
     1981 aatgt
//
LOCUS       DBNFCK010000183         1965 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.185, whole genome shotgun sequence.
ACCESSION   DBNFCK010000183 DBNFCK010000000
VERSION     DBNFCK010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.185"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            77..370
                     /locus_tag="AC1PIW_001882"
     CDS             77..370
                     /locus_tag="AC1PIW_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HIP6606774.1"
                     /translation="MQKTLEKLKRSELFELLVEQAKEIESYQAKLVDLEKRIEYREAV
                     LGEIGSIAEASLAVTRIFEEAQLAANIYLEQVQKVTHKQSDNCEIGRTDEDGD"
     gene            360..962
                     /gene="lepB"
                     /locus_tag="AC1PIW_001883"
     CDS             360..962
                     /gene="lepB"
                     /locus_tag="AC1PIW_001883"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIP6606775.1"
                     /translation="MATNKNKDYVTISDLPSISIMSEELDKIRYKERYLETLKNTIFT
                     LVTVVALAVLIAMLWLPVLHIYVSSMSPTLKAGDMVATVKTNTLSTGDMVAFYYNNKV
                     LVKRVVATSGQWVNIDEQGNVYANGKTLDEPYVKDKAYGQTDIKLPYQVPEGQYFVMG
                     DHRSVSIDSRNTAIGSIGEEQLVGKLTFRIWPFHKMGVIK"
     gene            981..1367
                     /locus_tag="AC1PIW_001884"
     CDS             981..1367
                     /locus_tag="AC1PIW_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606776.1"
                     /translation="MKILWERLFKFVQQYFREKKTAKITMVLSIIMAFGISYLLILPA
                     ITLETDSQESVLQSVYNQPTTVEQPVQEEQAESQLAQVQSAGSLFAEHENITVQVNYE
                     EHTFEQPVRLQVLPSEDSTKYIGLVR"
     gene            complement(1368..1757)
                     /locus_tag="AC1PIW_001885"
                     /pseudo
     CDS             complement(1368..1757)
                     /locus_tag="AC1PIW_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558050.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1725..>1965
                     /locus_tag="AC1PIW_001886"
     CDS             1725..>1965
                     /locus_tag="AC1PIW_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HIP6606777.1"
                     /translation="MASQIRHDLSLLVFFLITGLLLLLYPTVSDYWNSHYQSEAVATY
                     SEKLLDISKEEKQKILENVSAYNNTLEKGTIPNLNI"
BASE COUNT          700 a          293 c          402 g          570 t
ORIGIN      
        1 tgttagaagt aaataagcat atattttgta gtgaaagcaa tataagcaaa aaggagagag
       61 gaagaagcaa tgttaaatgc aaaagacctt agaaaaactg aagcgttctg aattatttga
      121 actcttggtt gagcaggcaa aggaaataga gtcttatcaa gcaaaattag tagatttgga
      181 aaaaagaatt gagtatcgcg aagcggtttt gggagaaatt ggttcgatag cggaggcgtc
      241 tctagcagtt actaggattt ttgaagaagc acaattggct gcaaatattt atcttgaaca
      301 agttcaaaaa gttacacata aacaatctga taattgtgaa atagggagaa cagatgagga
      361 tggcgactaa taaaaataag gattatgtta ccatatcaga tttacccagt atatcaataa
      421 tgtcagagga gctggataaa atacgatata aagagcggta tttagagaca ttaaaaaata
      481 ctattttcac gcttgttact gtcgttgcac ttgcagtatt aatagccatg ttatggttgc
      541 cagttctaca tatttatgta agttccatgt ctcctactct gaaagcaggt gatatggtag
      601 caactgtgaa gacaaataca ctttcaactg gagatatggt tgcattctac tacaataata
      661 aggttttggt aaaaagagta gtagcaactt ctggtcagtg ggtaaatata gatgagcagg
      721 ggaatgttta tgcaaatgga aaaacattag acgaacccta tgttaaagat aaggcctatg
      781 gtcaaactga tattaaacta ccgtaccagg ttccagaagg tcaatatttt gtaatgggag
      841 atcatcgcag tgtatctatt gattctagaa atacagctat tgggtcgatt ggtgaggagc
      901 aactggttgg aaagttaacc tttcggatat ggccatttca taaaatggga gtaatcaaat
      961 aaaggaagga gatataaaac atgaaaatat tatgggaaag attgtttaaa tttgttcagc
     1021 aatactttcg agagaaaaag actgcaaaaa taactatggt gttgtcaata attatggctt
     1081 ttggaatatc atatttgctg attttaccag ccattacttt agagacagac agtcaggaga
     1141 gtgttctgca atcagtctat aatcagccaa caacagtaga acagccagtt caagaagaac
     1201 aggcggagtc acaattggca caggttcaat ctgctggaag cttattcgct gaacatgaaa
     1261 atataactgt acaagttaac tatgaggagc atacatttga acagccagtt cggttacagg
     1321 ttttaccctc agaagatagt acaaaatata ttggacttgt tcggtaacta catcatctca
     1381 tggttgatga taccacaaat caactcagtt cttaattcaa accgttttct acgattacga
     1441 tagggctgtg ccatgatttt aaaggtttta aatttggcat taaagtgttc aacttcaata
     1501 cggattttag ctatctctcg attcaaccgt ctatcctctt cggttaagag gtgatgttta
     1561 gaatgcttag caggaataaa ggtattttcg tgaaatttca agatgcctaa ataccctaaa
     1621 tctacgaaag caagggtatt ttggggcaaa ctatagccaa tactctcctt aaaaagggta
     1681 aaatcatgcg tatgaccatc tgaaaaagcc aattgacaag cacgatggct agtcaaatca
     1741 ggcatgattt gagtctgcta gtctttttct taataactgg cctattattg ttattatatc
     1801 caacagtaag tgattattgg aactcacatt atcaatcaga agcagtagct acttattctg
     1861 agaaattatt ggatatttca aaagaagaaa aacaaaaaat tttagaaaat gtcagtgcat
     1921 ataacaacac gctcgaaaag gggacaatac ctaatttaaa tatag
//
LOCUS       DBNFCK010000184         1949 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.186, whole genome shotgun sequence.
ACCESSION   DBNFCK010000184 DBNFCK010000000
VERSION     DBNFCK010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.186"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..>1949)
                     /locus_tag="AC1PIW_001887"
     CDS             complement(<1..>1949)
                     /locus_tag="AC1PIW_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhamnan synthesis F family protein"
                     /protein_id="HIP6606778.1"
                     /translation="AKFFWRIPKYIKRTLLALQRDKQRRNNYSDIKNRYLIYTIYEHQ
                     SSLQDYKVIFLEALAKISRDVLIVVNGKLPQADINRLAQFGKVLERDNEGYDVAAFRH
                     GIIHTGKEALQQYNQLILVNDTNIGPFRDLEEVFSEFNSDQLDFWGISMGEEQLDFTG
                     YNPYGKIPKHLQSYFVVIENSLLRYEGFYDYWEKLSDTDSRNKAIGKHETVFAKYFYD
                     RGFKYDALIKDTKDSALYIHPFKLLKQGCPLVKYSAFRNYDREQYFWHGLERESEIPD
                     LMEYIAKETDYPIEVVSSILEDFKTRENQSYILIIDGVENIIPQCTRYRVLNKAEQLR
                     ELGYTVRVINNSLVQLQDAQFASHIIIYRAPFNDMLKEICRAAHIKNRPVYFDIDDLV
                     FDTKFTDELEFTQGLSKREKKGYDTSVLAYKKMLSLCDYAITSTSKLKDELEQYKNKV
                     ILNRNVMSKELVERSLQVKKNSNDNKVKIGYFSGSITHNENFDLISQALLNLLQKYPQ
                     VELHIVGYLDIPKPFQKFKKQIVSHEYVDWRKLPILISQVDINLAPLVTTTFNEAKSE
                     IKWIEAAAVKVVTVASNLGAFEEMIQDGVTGVLADDNEWESKLERLILEQDLRAQIAE
                     NAFEFVMNHCTTANRINDFLKEELV"
BASE COUNT          618 a          388 c          266 g          677 t
ORIGIN      
        1 gactaactct tctttcaaaa aatcattaat tctatttgct gtagtgcaat gattcattac
       61 aaactcaaaa gcattttctg ctatttgtgc tctcaagtct tgttcaagaa tcaatctctc
      121 caatttactt tcccattcgt tatcatctgc tagaactcca gtcactccat cttgaatcat
      181 ctcttcaaat gctccaagat tacttgcaac tgtcacaact ttaactgctg ccgcttctat
      241 ccatttaatt tccgactttg cttcattaaa ggttgtggtt acaagcggcg ccaagttaat
      301 atcaacttgt gaaattaaaa ttggaagctt tctccaatca acatactcat gactcacaat
      361 ctgttttttg aatttttgga atggtttcgg aatgtctaaa tatcctacta tatgtaattc
      421 cacttgcggg tacttttgaa gtagatttag caatgcttga ctaattaaat caaaattttc
      481 gttgtgtgta attgaccctg agaaatatcc tattttaact ttattgtcgt tcgaattttt
      541 cttaacttgt aaactacgct caactaattc tttagacatg acgttgcgat tcaaaataac
      601 cttgttttta tattgttcga gctcgtcttt caatttcgaa gtcgatgtga tagcataatc
      661 gcagagagaa agcatttttt tgtaagctaa aacacttgta tcatagcctt ttttctctct
      721 cttgctcaaa ccttgggtaa attcaagttc atctgtaaat tttgtatcaa aaaccaaatc
      781 atcaatatca aaataaacag gtctgttctt tatgtgtgct gccctacata tttctttcag
      841 catatcattg aatggagcac gataaataat aatatgacta gcaaactgtg catcctgaag
      901 ttgtacaaga gaattattta tcactcgaac tgtataacca agttctcgca attgctcagc
      961 cttatttaac acacgataac gtgtacattg tggaataata ttttcgacac catcaataat
     1021 caaaatataa gattgatttt ctctagtttt aaaatcttct aatatagaag acaccacttc
     1081 gatcggatag tctgtttcct tagcaatata ctccatcaaa tccggaatct cggattctct
     1141 ttctaaacca tgccaaaaat attgttctct gtcataattt ctaaaggccg aatacttaac
     1201 taatggacaa ccttgtttta ataatttaaa cggatgaata tacaatgcac tatctttcgt
     1261 gtctttaatc aatgcatcat acttaaagcc tcgatcataa aaatattttg caaacacagt
     1321 ttcatgtttt ccaattgcct tattacgaga atccgtatca gagagctttt cccaataatc
     1381 ataaaatcct tcatacctaa gcaagctatt ttcaataaca acaaaatatg attgtaagtg
     1441 cttaggaatt ttaccataag ggttataacc agtaaagtct agctgctcct cacccatgga
     1501 aattccccaa aagtctagct gatcggaatt aaactcagag aaaacctctt ccaaatctct
     1561 aaaaggacca atattcgtat cattaaccaa gatgagctga ttatattgtt gtaacgcctc
     1621 ttttccagta tgtattatcc cgtgtcggaa agccgcaacg tcatagccct cattatctct
     1681 ttccaaaacc ttaccaaatt gtgctaaacg atttatatca gcctgtggaa gtttaccatt
     1741 tacaacaatt aaaacatctc gagaaatttt agccaatgcc tctaaaaaaa ttactttata
     1801 gtcctgtaaa gacgattgat gttcataaat tgtgtaaatc aagtaacgat ttttgatatc
     1861 tgaataatta tttctacgtt gcttatcacg ttgcaaagct agtagagttc gctttatata
     1921 ttttggaatt ctccagaaaa actttgcaa
//
LOCUS       DBNFCK010000185         1904 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.187, whole genome shotgun sequence.
ACCESSION   DBNFCK010000185 DBNFCK010000000
VERSION     DBNFCK010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.187"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            44..>1904
                     /locus_tag="AC1PIW_001888"
     CDS             44..>1904
                     /locus_tag="AC1PIW_001888"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIP6606779.1"
                     /translation="MTLVTNLKQHSHIITTALCLALILIGILLFQTGQGWAPILFISA
                     FVIGGYQSAKEGISELIFDKHLSVDLLMILAAIGSGLIGYWMEGALLIFIFSLSSTLE
                     ELAMKKSKNAIAALMNMTPPTARKIEENGDITVLDTAAIRIGDLLQVRKGDTVPLDAT
                     LISPQSIFDESMITGEPLPAEKMAGDAVIGGTINQGPTVTVQVTAEKGDALFDKIVQM
                     VENAQESKSKTATFIENMEDTYVKVVLVVVPLFILLAHFALGWDWLTAFYRGMILLTI
                     ASPCALVASSSPATLSAISRAARKGMIIKGGDIADNIANLEAIVFDKTGTLTIGKPEV
                     VGATYLGDEELIKQVVQAVEKQSSHPIAQALMTYTADSSAIALQSLEDVTGKGLVAGY
                     EGDSWKIGKAGFVADSLVSPLSAGLLAQIDEAESIGKTLVYVSQNDVLVAIFMVEDSL
                     KPESKQLIAQLKEMGVTPILLTGDQEKTARYVASQVGIDRVIANCLPTDKAAVIQELQ
                     TEFASVGMVGDGINDAPALAQANVSYAMGSGTDIAMESADIVLMEDLTRIPYSIRLSK
                     KMRGIIKQNIIFALSVIALLIISNLFQSINLPLGVVGHEGSTILVILNGLRLLY"
BASE COUNT          481 a          480 c          463 g          480 t
ORIGIN      
        1 acttagttaa atgataataa ttctaaataa ggaagagcaa actatgacac tcgtaaccaa
       61 cctcaaacaa cacagccaca tcatcacgac cgctctttgc ttggcgctca ttctaatagg
      121 tatccttctg tttcagactg gacagggatg ggctcctatc ctcttcattt cagcctttgt
      181 catcgggggc taccaatcag ccaaggaagg catttctgag ctgattttcg acaagcacct
      241 gtctgtcgac ctgctcatga ttttagctgc tatcggctct ggtctaattg gctactggat
      301 ggagggggct ctactcatct tcatcttctc cctttcttct actctggaag aattagccat
      361 gaaaaaaagc aagaatgcga tcgctgcttt gatgaacatg acccctccaa cagctcgcaa
      421 aatcgaggaa aatggtgaca ttactgtctt agacaccgca gccatccgca ttggcgacct
      481 cctgcaagtc cgcaagggtg acacggttcc actggatgct accctcatca gtccgcaatc
      541 catctttgac gaatccatga ttacaggcga gccgctccct gcggagaaaa tggctggcga
      601 tgctgttatc ggcggaacca ttaaccaagg tccaactgtg accgtacaag ttacggctga
      661 aaaaggcgat gccctcttcg ataaaatcgt ccagatggtt gaaaacgccc aagaatccaa
      721 atcaaaaaca gcgactttca ttgaaaatat ggaagatacc tatgtcaagg ttgtcttggt
      781 ggtggtacca ctcttcatcc tccttgctca ttttgcactg ggctgggact ggttaactgc
      841 cttctaccga ggcatgattc tcttaaccat cgcttctcct tgtgccctcg tagcttcttc
      901 ttcacccgca acactttctg ccatcagccg tgcggcacgt aagggcatga ttatcaaggg
      961 cggtgacatt gcggacaaca tcgccaatct agaggccatc gtctttgata agacgggtac
     1021 cctgaccatt ggtaaacctg aagttgtagg tgcgacctat cttggcgatg aagagcttat
     1081 caagcaggtt gttcaggcag ttgaaaaaca atcaagccac ccaatcgctc aggctctcat
     1141 gacctatacg gctgacagct ctgctattgc ccttcaaagc ctagaagacg tgacaggaaa
     1201 aggcttggtg gcaggctatg agggcgacag ctggaaaatc ggtaaggcgg gatttgtggc
     1261 ggacagcctg gtgagtcccc tgtctgctgg cttgctggca cagatcgatg aggcggaaag
     1321 cattgggaaa accctggtct atgtcagcca aaatgatgtg ctggtggcga tcttcatggt
     1381 ggaagatagt ttgaaacctg agagcaagca gctgattgcc cagttgaaag aaatgggcgt
     1441 gacgccaatt ctcttgacgg gtgaccaaga aaagacggct cgttacgtgg cgagtcaggt
     1501 cggtattgac cgtgtgattg ccaactgtct gccgacggat aaggctgctg ttatccaaga
     1561 attacaaacc gagtttgcgt ctgtcggcat ggtcggagac ggcatcaacg acgctcccgc
     1621 cctggctcaa gccaatgtca gctatgctat gggaagcgga acggacatcg ctatggagtc
     1681 agctgacatc gtactcatgg aagacctgac gagaattcct tactccattc gtctctctaa
     1741 gaaaatgcgg ggcatcatca agcagaacat catctttgcg ctatctgtca ttgcccttct
     1801 tatcatctcc aacctcttcc agtctatcaa cctcccactc ggtgtggtgg gccacgaagg
     1861 atcaacgatt ttggtgattt tgaatgggtt gagactattg tatt
//
LOCUS       DBNFCK010000186         1900 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.188, whole genome shotgun sequence.
ACCESSION   DBNFCK010000186 DBNFCK010000000
VERSION     DBNFCK010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.188"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..>1900)
                     /locus_tag="AC1PIW_001889"
     CDS             complement(<1..>1900)
                     /locus_tag="AC1PIW_001889"
                     /inference="COORDINATES: protein motif:HMM:NF012570.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HIP6606780.1"
                     /translation="SMTKIKIISNPFHKSIVFQKQQPITEVWEEITFESNPNSRLVRE
                     NIQKGVLSFHIQEIVDILVAEYDDGTGIDLLFEGNSDEFQELKTFVQSLNNSSISLKK
                     SAYSLTNARDILPEVLSIFDKTQVILPEELEHDAAIQKFQDATKPQIPLLVLGNYSAG
                     KSTFINSLVGYELLPASDQPTTAKIIQIEKFAEKWIEIEGTLLRNKVCIRIDEDGFEV
                     EGDRIDETIIKDLESIRQSLPQTTPQILARAVLAYLNAREDSDLNIITVKTAFTDSPL
                     SKSSHRYIIFDTPGSNSSTNMEHLQLLREAMAGMSNGLPIFISEYTSLDTVDNDKLYQ
                     ELEQIDGIDLRYTILVVNKADTAALSTFQDHQVLNQALPRKLSTKRVYFLSSLMGLGS
                     KTSGLFQEPDYERVYQKSLAEFTDASNPYYQQLYTYNILPTQQKLAQELQSKEERNLL
                     YANSGLYSLESEIEVFSTKYAAYNKCVQADQYLQHIISQTQSIIEDSKKTIEQEKDRL
                     EKKLEKDKLALVNDLKRQQADFFNSSSTVYQQNLNEKVRDEKHYAPLQLFIKERDSLW
                     SKAVDDFQLKSYQKEMAESPKKALNNIKNSLGKAWDKKNIEILGSAFVNGFNDTGKVF
                     SKVDDYNK"
BASE COUNT          552 a          376 c          308 g          664 t
ORIGIN      
        1 attttattat agtcatcaac cttcgaaaac acttttcccg tatcgttaaa accatttaca
       61 aatgctgaac ctaggatttc aatatttttc ttatcccagg cttttcctaa actattcttg
      121 atattgttta gggctttctt aggagattct gccatttctt tttgatagga tttcaattga
      181 aaatcatcaa ctgccttact ccatagagag tcccgctctt ttatgaacag ttgtaacgga
      241 gcataatgtt tctcatccct tactttctca ttcaagtttt gttggtatac cgtcgatgag
      301 gaattgaaaa aatcagcttg ttggcgtttt aaatcattca ccaatgccag cttgtctttc
      361 tccaactttt tctctaaacg atctttttct tgctcaattg ttttcttgga atcttcaatg
      421 attgactgcg tttgagaaat aatatgctgc aaatactggt ccgcctgtac acatttatta
      481 taagccgcat acttggttga aaaaacctca atttccgact ctaaactgta cagtccactg
      541 ttggcataga gaagatttct ctcctcctta gactgcaatt cctgagccag tttctgctga
      601 gttggaagaa tattataagt atacaactgt tgataataag gatttgaagc gtctgtaaac
      661 tcagccaaac ttttttgata aactctctca taatctggct cttggaaaag accgctggtt
      721 tttgacccca agcccattag agaggatagg aaataaactc tttttgttga taattttctt
      781 ggaagggctt gatttaatac ctgatgatct tgaaaagttg ataaggccgc agtgtctgct
      841 ttgttaacaa ctagtattgt ataccgtaaa tcaattccgt ctatttgttc taattcctga
      901 tagagtttat cattgtctac agtatctaag ctagtgtatt ccgatataaa aataggcaaa
      961 ccattgctca tacccgccat agcctcacga agaagctgca aatgctccat attggtcgaa
     1021 gaattggaac ctggagtatc aaatatgatg tagcgatgag aagattttga cagtggacta
     1081 tcggtaaacg ctgttttcac agtgataata ttcaaatcac tatcttccct agcattcaaa
     1141 taagccaaca cagcccgtgc tagtatttgc ggagttgttt gtggcaaact ctgacgaatg
     1201 gattctaaat ctttgataat tgtctcatca atcctgtcgc cttcaacctc aaagccgtcc
     1261 tcatcaattc ggatacaaac cttatttctt agaagtgttc cttctatttc aatccatttt
     1321 tcagcaaatt tctcaatttg tataattttc gctgttgtag gttggtcact ggcaggcaat
     1381 aactcatatc ctaccaatga attgataaaa gtagacttcc ctgcactata atttcccaaa
     1441 accaataatg gaatctgagg ttttgttgca tcttgaaact tttgtatggc tgcgtcatgt
     1501 tcaagttctt caggaagaat gacctgagtt ttatcaaaaa tagaaagaac ttcaggtaga
     1561 atatctcgtg catttgtcaa gctataagca gatttcttca aagaaataga agaattattt
     1621 aacgattgga caaatgtttt caactcctga aattcatcac tatttccttc aaataacaag
     1681 tcaatccctg ttccatcatc atattctgcc accagaatat ctactatttc ttggatatga
     1741 aatgatagca cccctttttg aatattttcc cttaccaaac gactatttgg attggattca
     1801 aatgtaattt cctcccaaac ttctgtaatc ggttgttgct tttgaaaaac aatactttta
     1861 tgaaatggat tgctgattat ttttatcttt gtcatactag
//
LOCUS       DBNFCK010000187         1870 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.189, whole genome shotgun sequence.
ACCESSION   DBNFCK010000187 DBNFCK010000000
VERSION     DBNFCK010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.189"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            177..>1870
                     /locus_tag="AC1PIW_001890"
     CDS             177..>1870
                     /locus_tag="AC1PIW_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy0128 family protein"
                     /protein_id="HIP6606781.1"
                     /translation="MNTKKWRTSLLIPGIVLFGTVALVNNVSAQEVKNTIISAKQPDG
                     GQATSKAVNVKIPAVVRLFGRELLENEFKFELREANGEELPVLDTAQNTKEGQVRFKN
                     LSFDKPGKYWYTISEVKDELGGIEYDSKYIVAKITVEDRNGQLQAMIEFIDNDNVFNN
                     FYTPAPAAASLSIKKVLEGRTLNTGEFEFVLKNEKGDEIEKVSNQADGSVNFSALTFT
                     KEGTYTYTVSEVDGGLGDIIYDKSDIKATVTVKDNNHGQLVSTVTYENSDQIFENILN
                     PGKLIAPTTDSVITDNEVSKEAMTGKEKGNIEPPKEQIANEEKDNIEASEKQMPSIVN
                     DMVVTPEKQMTNKENDKVVISEKQMPSVVNENAVTPEKQMTNKENDNIETSEKQMPSV
                     VNENAVTPEKQMTNKEKDNIETSEKQMPSIVNDMVVTPQEQMANKENDKVVISEKQMP
                     SIVNDMVVTPQEQMANKENDKVETSEKQMPVNEKDNAVTPEKQMANKEKENIETSKKQ
                     IPVNENNQNGTVEENSNTKPTTEKTDKQETSTFKTETAKQILPVTGEKGSLWLLTSG"
BASE COUNT          743 a          304 c          381 g          442 t
ORIGIN      
        1 ttttaaaagc agaaggacag cttgaatgtt ttttagaaga tgttataatt tacgaatggt
       61 gcacattctg gcactaagtg ttcaaatttt attgaggact ttagatgtaa aatttgcatg
      121 ttataacgac acagattagc tacataaatc atgctttgag aaaggaaaaa aggataatga
      181 atactaagaa atggagaaca tcgctcctaa taccaggaat agtattattt ggaactgttg
      241 ctctagtgaa taatgtatcg gcacaagaag taaaaaatac catcatcagc gcaaaacaac
      301 ctgatggggg acaggctact tcaaaggcgg ttaatgtcaa aataccagca gtagtacgac
      361 tatttggtcg tgagcttcta gaaaatgaat ttaaatttga gcttagagaa gcgaatggcg
      421 aggaactccc tgtccttgat acagctcaaa atacaaaaga gggtcaagtt agatttaaaa
      481 atctatcatt cgataagcct ggcaaatact ggtatacaat ttcagaagta aaagatgagc
      541 ttggtggtat tgagtatgat tcgaaatata ttgtagcaaa aataactgta gaagatcgaa
      601 acgggcaatt acaggcaatg atcgaattta ttgataatga caatgtcttt aacaatttct
      661 atacacctgc tccagctgct gctagtcttt cgataaaaaa agtcctcgag ggacgtacct
      721 taaacaccgg tgaattcgaa tttgttttaa aaaatgaaaa aggcgatgaa atcgaaaagg
      781 taagcaatca agcagatggt tctgtaaact ttagtgccct aacatttaca aaagagggaa
      841 cctataccta cactgtttca gaagttgatg gtggacttgg cgatattatc tatgacaaat
      901 cagatattaa ggccactgtt actgtgaaag ataacaatca cggacaacta gtctcaacag
      961 tgacttatga aaatagcgat caaatcttcg agaatatttt gaatcctggg aagttaatag
     1021 cgccaaccac ggatagcgtt attactgata atgaagtctc taaggaagca atgaccggta
     1081 aagagaaggg aaatatcgaa ccccctaaag agcaaatagc taatgaagag aaggataata
     1141 ttgaagcctc tgaaaaacag atgccaagca ttgtgaacga catggtcgta acacctgaaa
     1201 agcaaatgac taataaagag aacgataagg ttgtaatctc tgaaaaacaa atgccgagtg
     1261 ttgtgaacga aaatgccgta acacctgaaa agcaaatgac taataaagag aacgataata
     1321 ttgaaacctc tgaaaaacag atgccgagtg ttgtgaacga aaatgccgta acacctgaaa
     1381 agcaaatgac taataaagag aaggataata ttgaaacctc tgaaaaacaa atgccaagca
     1441 ttgtgaacga catggtcgta acacctcaag aacaaatggc taataaagag aacgataagg
     1501 ttgtaatctc tgaaaaacag atgccaagca ttgtgaacga catggtcgta acacctcaag
     1561 aacaaatggc taataaagag aacgataagg ttgaaacctc tgaaaaacag atgcctgtta
     1621 atgagaagga caatgccgta acacctgaaa agcaaatggc taataaagag aaggaaaata
     1681 tcgaaacctc taaaaaacag atacctgtta atgagaacaa ccaaaatggt acagtcgaag
     1741 aaaattcaaa cactaaacca acaactgaaa aaacagacaa gcaggagact tcaacattta
     1801 aaaccgaaac tgctaagcaa atcttaccag taactggtga gaaaggaagt ttatggttat
     1861 tgacaagtgg
//
LOCUS       DBNFCK010000188         1711 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.190, whole genome shotgun sequence.
ACCESSION   DBNFCK010000188 DBNFCK010000000
VERSION     DBNFCK010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.190"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(3..1496)
                     /gene="sly"
                     /locus_tag="AC1PIW_001891"
     CDS             complement(3..1496)
                     /gene="sly"
                     /locus_tag="AC1PIW_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin suilysin"
                     /protein_id="HIP6606782.1"
                     /translation="MRKSSHLILSSIVSLALVGVTPLSVLADSKQDINQYFQSLTYEP
                     QEILTNEGEYIDNPPATTGMLENGRFVVLRREKKNITNNSADIAVIDAKAANIYPGAL
                     LRADQNLLDNNPTLISIARGDLTLSLNLPGLANGDSHTVVNSPTRSTVRTGVNNLLSK
                     WNNTYAGEYGNTQAELQYDETMAYSMSQLKTKFGTSFEKIAVPLDINFDAVNSGEKQV
                     QIVNFKQIYYTVSVDEPESPSKLFAEGTTVEDLKRNGITDEVPPVYVSSVSYGRSMFI
                     KLETSSRSTQVQAAFKAAIKGVDISGNAEYQDILKNTSFSAYIFGGDAGSAATVVSGN
                     IETLKKIIEEGARYGKLNPGVPISYSTNFVKDNRPAQILSNSEYIETTSTVHNSSALT
                     LDHSGAYVAKYNITWEEVSYNEAGEEVWEPKAWDKNGVNLTSHWSETIQIPGNARNLH
                     VNIQECTGLAWEWWRTVYDKDLPLVGQRKITIWGTTLYPQYADEVIE"
BASE COUNT          491 a          367 c          284 g          569 t
ORIGIN      
        1 gattactcta tcacctcatc cgcatactgt gggtataacg ttgttcccca gatggttatt
       61 ttacgttgac caacaagtgg taaatctttg tcataaactg ttctccacca ctcccatgct
      121 aatcctgtac attcttgaat attgacatga agattgcgag catttcctgg aatttgaatg
      181 gtttcactcc agtgtgaggt cagatttaca ccattcttat cccaagcttt tggttcccaa
      241 acttcttctc cagcttcatt gtaagatact tcttcccaag taatgttgta tttcgcaaca
      301 taagcacctg aatgatccaa tgtcaatgca ctgctattat ggactgttga agttgtttct
      361 atgtactctg aattgctcaa aatctgagca ggtctattgt ctttgacaaa gttggttgaa
      421 tacgaaatcg gaacacctgg attgagtttt ccgtatcttg caccttcttc aataatcttc
      481 ttcagtgttt caatatttcc gctcacaaca gtagccgcgc tacctgcatc cccaccaaaa
      541 atataagcag agaatgaagt atttttcaga atgtcttgat actcagcatt gccactaata
      601 tcaacgcctt tgatggctgc tttaaatgcg gcttgaactt gggtactcct actgctagtt
      661 tctaacttga tgaacataga gcgtccataa gaaacgctgg aaacataaac aggaggtacc
      721 tcatctgtta tcccatttcg tttcaaatct tctacagttg tcccttctgc aaaaagcttg
      781 cttggagatt ctggttcatc aacactaact gtataataaa tttgtttaaa gttaacaatc
      841 tgaacctgtt tttcacccga attcacggca tcaaaattga tatctaatgg tacagcaatt
      901 ttttcaaaag aggttccgaa cttcgttttc aattgtgaca tactgtatgc cattgtttca
      961 tcatattgaa gctctgcttg ggtattgcca tactctccag catacgtatt attccattta
     1021 gacagaaggt tattcacccc tgttcgaaca gtacttcttg ttggagaatt tacaacagtg
     1081 tggctatccc cattggccaa accaggtaaa ttcaaactaa gcgtcagatc tccccgcgca
     1141 atactgataa gcgttggatt attatccaga agattttggt cagcacgcaa taaagcacct
     1201 ggataaatat ttgcagcctt agcatcaata acagcaatat ctgcactatt gttcgtaata
     1261 ttcttctttt ctctgcgaag tactacaaaa cgtccgtttt ctaacatacc agttgttgct
     1321 ggcggattat caatgtattc tccctcattt gtaagaatct cttgtggctc gtaagtcaag
     1381 ctttgaaaat actgattaat atcttgtttg gaatctgcaa gaacactcaa tggtgtgacc
     1441 cctacgagtg ccaaactgac tattgagctt aaaatcaagt gcgaactttt tctcataagt
     1501 tttatccttt catgttttca ctaatgaaat gaatagacat aaaaaatggt attaaaaaaa
     1561 caaacgtttg cgagttcatt ctgcaccttt ttagataagt tgtcaagact ccttgagatg
     1621 aaaaacagac aaaaaacaga ttacgcctct ctatttgttg tttttccaac aatttccgga
     1681 atatttctgt aaacaaccta ttcattttct g
//
LOCUS       DBNFCK010000189         1704 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.191, whole genome shotgun sequence.
ACCESSION   DBNFCK010000189 DBNFCK010000000
VERSION     DBNFCK010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.191"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..555
                     /locus_tag="AC1PIW_001892"
     CDS             <1..555
                     /locus_tag="AC1PIW_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HIP6606783.1"
                     /translation="LNITDTGIMGYVEIPKLQTTLPIYHGTDDAVLQVAVGHLAGTSL
                     PIGGQGSHAAISGHRGLPSAKLFSDLDQLVIGDTFMIQVLDNTLTYEIDKIITVTPDD
                     VSPLMIDPNEDFVTLVTCTPYGVNSHRLLVRGHRIENQLLARQVVSEGSIVKPILVSP
                     FIALCLLGLLWSLFFIKKEVNLFM"
     gene            685..849
                     /locus_tag="AC1PIW_001893"
                     /pseudo
     CDS             685..849
                     /locus_tag="AC1PIW_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003104139.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            938..1348
                     /gene="nmlR"
                     /locus_tag="AC1PIW_001894"
     CDS             938..1348
                     /gene="nmlR"
                     /locus_tag="AC1PIW_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stress response transcriptional regulator NmlR"
                     /protein_id="HIP6606784.1"
                     /translation="MNIKEVSDVTGLSADTIRYYERVGLIPKIARKSSGVRDFVENDV
                     AVLEFVRCFRSAGMSIERLIEYMGLVQAGDSTVEARIDLLKEEREVLQSRLSKIQQAL
                     ERLDYKIENYQTILRGAENQLFTDGSGSCKKDRE"
     gene            complement(1374..1685)
                     /locus_tag="AC1PIW_001895"
     CDS             complement(1374..1685)
                     /locus_tag="AC1PIW_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HIP6606785.1"
                     /translation="MPLRHYQSHEYQYEEYIPKEKKANFQTYQAKNANKERLKELLFF
                     VNIAFFSLITVIASYVYLSVGIPVFIAIILASATGFLGLRLVQIGLKKKFKPKQVIRK
                     N"
BASE COUNT          520 a          293 c          360 g          531 t
ORIGIN      
        1 ttaaatatca cagatactgg tattatgggc tatgtagaaa ttcctaagtt gcagacaacg
       61 ttacctattt atcacggaac ggatgatgcc gtattgcagg tagcagtagg tcaccttgct
      121 gggacttccc ttcccattgg cggtcaggga agtcacgctg ctatttcagg acatcgggga
      181 ttgccttctg caaaactgtt ttctgattta gatcagctgg ttatagggga cacatttatg
      241 atacaagttt tagataacac tctgacatac gaaattgata aaattattac ggttactcca
      301 gatgatgtat ctcctttgat gatagatccg aatgaggatt tcgtaaccct tgtgacttgc
      361 acaccttatg gtgttaatag ccaccgatta ttagttagag gtcatcgaat tgaaaatcaa
      421 ctacttgcta ggcaagtagt ctcagaagga tcaattgtaa aacctatact ggtttctcct
      481 tttattgctt tatgtttatt aggactactt tggagtttat tttttataaa aaaagaagta
      541 aatttgttta tgtgacttag ataatatcgc aaagaatgtc aaaccattcg actttgttct
      601 ttagctttta agcttaggaa agggaggttt ttcaactccc aatcatccaa aaggtgaaat
      661 agaagataaa ccaaatgacg atatgtcaat gtcctctata cgccagaaaa aggtggcaag
      721 cgagcggtcg gctacggcat tgaaattccc aaagaatttg aaggcaaatt caagtttgcc
      781 cgccaacgct ccaaactagc tggggaaatc cgtggcactt tcttcgttgt caagggtaac
      841 tatttatagg ggagaaaatc tggggaaact cagatttttt gtcgcatact tgacttcaag
      901 gtatataata gatatacaaa acgaagggag aatgaatatg aacattaaag aagttagtga
      961 tgtaacaggt ctatcggcag ataccattcg ctactatgag cgggtagggc ttattcccaa
     1021 aattgcaaga aaatcttctg gagtaaggga ctttgtggaa aatgatgtag ccgttttgga
     1081 atttgttcgc tgttttagga gtgctggtat gtctattgag cgcttgattg agtatatggg
     1141 cttggtgcag gctggcgata gtactgtgga agcccggatt gatttactaa aagaggaacg
     1201 ggaagtcctg cagtcacgct tgtcgaaaat tcagcaagct ctagaacgtt tagattataa
     1261 gatagaaaat taccagacta tattaagagg tgcagaaaat caattgttta cagacggcag
     1321 tggttcatgc aaaaaagaca gagaataact ttctctgtct tcatacttca ttattaattt
     1381 tttctgataa cttgtttggg tttaaatttc ttctttagtc caatttgaac caaacgtagt
     1441 ccaagaaaac ccgtcgcact tgccaatatg atggcaataa aaacaggaat gcctacagat
     1501 agatagacat aactagcgat gactgttatc aaactaaaaa atgcgatatt aacaaaaaac
     1561 aacaattcct ttagacgttc tttattggca ttttttgctt gataggtttg aaaattagcc
     1621 tttttctctt tcgggatata ctcttcgtac tggtactcat gggactgata gtgtcttaat
     1681 ggcatatcgt ctccttttct gctc
//
LOCUS       DBNFCK010000190         1687 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.192, whole genome shotgun sequence.
ACCESSION   DBNFCK010000190 DBNFCK010000000
VERSION     DBNFCK010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.192"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..334)
                     /locus_tag="AC1PIW_001896"
     CDS             complement(<1..334)
                     /locus_tag="AC1PIW_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HIP6606786.1"
                     /translation="MKHQSILFYSRCLLAILAITGTALEIIKYCIGMLMYYTVQSNLL
                     VSLFAVYMVYAMSKGVDLQTSRFLRIKAAVTMSIMITCVVYHFMLAPLADDFWRLENL
                     LCHYIVPLY"
     gene            complement(382..720)
                     /locus_tag="AC1PIW_001897"
     CDS             complement(382..720)
                     /locus_tag="AC1PIW_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HIP6606787.1"
                     /translation="METLPNCPKCNSEYVYEDGALLVCPECAYEWNPADVAEEESGPV
                     AIDANGNRLADGDTVTLIKDLKVKGAPKDLKQGTRVKGIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVKKI"
     gene            complement(801..>1687)
                     /locus_tag="AC1PIW_001898"
     CDS             complement(801..>1687)
                     /locus_tag="AC1PIW_001898"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIP6606788.1"
                     /translation="RVEVGGIRLDKALSDLTDLSRSVANEQIKAGQVLVNGQPKKAKY
                     SVQVGDVLTYQIPEVEEIDYVAEDIPLEIVFQDEDVAVVNKPQGMVVHPSAGHTSGTL
                     VNALLYHVKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHTKLAAELKDKKSLRK
                     YWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKEAVTRFQVLERFGDYTLVELT
                     LETGRTHQIRVHMAYIGHPVAGDEAYGPRKTLKGHGQFLHARTLGFTHPRTGEVVEFT
                     AEAPAIFQEALEKLRKAE"
BASE COUNT          498 a          406 c          302 g          481 t
ORIGIN      
        1 aatataaggg aacaatataa tggcagagta gattttcaag tcgccagaaa tcgtctgcca
       61 aaggtgccaa cataaagtga tagactacac aggtaatcat gatagacatg gttacagcag
      121 cctttatccg aagaaaacgt gacgtctgta aatccacacc cttactcatg gcatagacca
      181 tatagacagc gaacagagat actaataaat tagattgtac cgtataatac atgagcatcc
      241 cgatacaata tttaatgatt tctaaggcgg tacctgtgat ggctaaaata gctaacaggc
      301 atctactgta aaacaaaata ctttgatgtt tcataatatc ctttcaaaaa caggaaaaga
      361 ctggttaacc agtcctctct cttagatttt cttaacaaat tcagatttca acttcatggc
      421 accgaaacca tcgattttac aatcaatatt gtgatcacct tcaacgatac ggatgccttt
      481 aacacgtgta ccttgtttaa ggtccttagg cgcccctttc actttcaaat ctttgataag
      541 cgtcacagta tcgccgtccg ccaaacgatt accgttagca tcaattgcaa caggtccact
      601 ttcttcctca gctacatctg ctgggttcca ttcgtatgca cactcagggc aaaccaagag
      661 ggcaccgtct tcatagacat attcagaatt acattttgga caatttggta aagtttccat
      721 tcttactctc cttaacattt ttcatgttct agtatagcat ttgttgaaaa aatgggcaag
      781 atgataaggt tttcatacta ttattcagcc ttgcgcagtt tttcaagtgc ctcctgaaaa
      841 atagctggcg cttctgccgt aaattcaaca acctcaccag ttctcggatg ggtaaatcca
      901 agcgttctgg catgaagaaa ttggccatgc ccttttagtg ttttccttgg gccgtaagct
      961 tcatcacccg caaccggatg accaatataa gccatgtgga cgcgaatttg gtgagttcgt
     1021 cctgtttcca aggtcaattc caccaaagtg tagtcaccaa aacgctccag aacctgaaaa
     1081 cgtgtcaccg cttctttccc tttggcagtc accgcttgtt tcttgcggtc tttttcagaa
     1141 cgaccaatcg gcgcctcaat tactccgcga tcgtttggta gattgccgtg tacaatggcc
     1201 caatacttac gaagggattt cttatctttc aattcagcag caagctttgt atgggcatca
     1261 tcgtttttag caatcatgag caagccagat gtatccttgt caatccgatg gacaatacct
     1321 ggtcgcaaga caccattgat gcctgacaaa tcttttacgt gataaaggag agcattaact
     1381 aacgttccag acgtgtgacc agccgacgga tgaacgacca tcccttgagg tttatttaca
     1441 acagcaacat cctcgtcctg aaagacaatc tctaaaggaa tatcctcagc aacatagtcg
     1501 atttcttcta cttctggaat ttgataggtt agaacatccc caacctgcac actatattta
     1561 gcttttttag gctgtccatt gaccaaaacc tgaccagcct taatctgctc atttgcaacc
     1621 gaacgagaca aatccgtcaa atctgacaat gccttatcca aacgaatacc accaacctca
     1681 actctta
//
LOCUS       DBNFCK010000191         1633 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.193, whole genome shotgun sequence.
ACCESSION   DBNFCK010000191 DBNFCK010000000
VERSION     DBNFCK010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.193"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            41..799
                     /locus_tag="AC1PIW_001899"
     CDS             41..799
                     /locus_tag="AC1PIW_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6606789.1"
                     /translation="MKSNKEQKYFWLTFSLIVALMIWWFLSNYDRTSRSFPNIILVWN
                     ALLIMIDKGFIFEDIFSSLTSVFFGFTSGFLLSAPNALLMAWYSPYRNIVKPWIQFIR
                     NIPPLAYVPLVVISAGVGLLPQVIVIAIATFLTMTVTIYQGIINIDPNLIKAARILGA
                     SDFEIFKRILVPSSIPFIMTAVRLGTSISLTTIIAAESTGATSGMGMRIRSLNNSFDS
                     SGMLLYIILIGVIGLLLNKLVDLIERKLTGWQQK"
     gene            784..1566
                     /locus_tag="AC1PIW_001900"
     CDS             784..1566
                     /locus_tag="AC1PIW_001900"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6606790.1"
                     /translation="MATEVKKIKVKIEDVSKSFQTNHGEVVALNQINLEIFENEIVTV
                     VGPSGCGKSTLLNLVAGLEPVTSGKIYCDDNEIVGIGVERGVVFQQYALFPWMSVLQN
                     VMFGPEMQGKSKLEASEIANKYIKMVGLQEFSNHYPKELSGGMKQRVAIARAYAVNPS
                     ILLMDEPFGALDALTRIQLQEELIEMLGQEQKTCFFITHDIEEAVLLGNRVIVMSPRP
                     GEIKEIVNVDLPFPRNQLTKFEKNFMDIKNHIWSLVYKDFLI"
BASE COUNT          539 a          219 c          322 g          553 t
ORIGIN      
        1 gaaacatttt ttatgtattt gtaactatgg gaggatgaag atgaagagca acaaagaaca
       61 aaaatatttt tggttgacat tctcattgat tgttgcattg atgatttggt ggtttttatc
      121 gaattatgat cgaacctctc gtagttttcc aaatatcatt ttggtttgga atgctttatt
      181 aatcatgata gacaaaggat ttatctttga agatatattt tcaagtctaa cttcagtttt
      241 cttcgggttc acgtcgggct ttctattgtc ggcgcctaat gcactcttaa tggcctggta
      301 ttcaccttat cgtaatattg tgaaaccttg gatacaattt atcagaaata ttccacctct
      361 tgcatacgtc cctttagttg taatttcagc tggggttggg ctattacctc aagttattgt
      421 aatagcgata gctacttttc tgactatgac tgtaacaatt tatcaaggca ttattaatat
      481 cgatccaaat ttaattaaag cagcaaggat tttaggagca tcagattttg aaatatttaa
      541 aagaattttg gttccctcat ctattccatt tattatgaca gctgtcagat taggtacatc
      601 gatttcttta acaactatca tagcagctga atctactggt gcaacatcag gtatgggaat
      661 gagaatacgg tccttaaata actcttttga ttcctcggga atgcttttgt atatcatatt
      721 gattggagtg attggtttac ttttaaataa gttagttgat ttgatagaaa ggaagttaac
      781 tggatggcaa cagaagtaaa aaaaattaag gtcaaaattg aagatgtttc aaaatcattt
      841 cagacaaatc atggtgaggt tgttgcacta aatcaaataa atttagaaat ctttgagaat
      901 gaaatagtta cagttgttgg tccatctggt tgtggtaaat ctactctact aaacttggtt
      961 gcaggtttgg aacctgtaac ttccggaaaa atttattgtg atgataatga aattgttggc
     1021 attggagttg agcgtggagt tgtttttcaa caatatgcat tatttccttg gatgagtgta
     1081 ctacagaatg taatgttcgg acctgaaatg caaggaaaat caaagttaga agctagtgaa
     1141 atagctaaca agtatattaa aatggttgga ttacaagaat tttccaatca ttatccgaaa
     1201 gaattatcgg gtggaatgaa gcaaagggtt gctatagcga gagcttatgc tgttaacccg
     1261 tcaattctat taatggatga gccctttgga gcattagatg cactaactag aattcagctt
     1321 caagaggagt tgattgaaat gttaggacaa gaacaaaaaa cttgtttttt cataacacat
     1381 gatattgaag aggcggtact attaggaaat cgagtgattg ttatgagtcc acgccctgga
     1441 gaaattaaag aaattgtgaa cgttgattta ccatttccaa gaaatcagtt gacaaagttt
     1501 gaaaaaaatt ttatggacat aaagaaccat atatggtcac ttgtttataa agattttcta
     1561 atataaaaaa gtgaaacagt agaattattt tgtgagaggg taaaatggct aaatatatga
     1621 aatataaaaa ctt
//
LOCUS       DBNFCK010000192         1614 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.194, whole genome shotgun sequence.
ACCESSION   DBNFCK010000192 DBNFCK010000000
VERSION     DBNFCK010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.194"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..355
                     /gene="yihA"
                     /locus_tag="AC1PIW_001901"
     CDS             <1..355
                     /gene="yihA"
                     /locus_tag="AC1PIW_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HIP6606791.1"
                     /translation="PGYGYAKVSKTERAKWGKMIEEYLTTRENLRVVVSLVDMRHEPS
                     ADDVQMYDFLKYYEIPVIIVATKADKIPRGKWNKHESMIKKKLDFDKNDQFVIFSSET
                     RHGYDQAWDAILSNI"
     gene            378..791
                     /gene="ndk"
                     /locus_tag="AC1PIW_001902"
     CDS             378..791
                     /gene="ndk"
                     /locus_tag="AC1PIW_001902"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002934976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HIP6606792.1"
                     /translation="MERTFFIIKPDALKRGLAGQILSRIERRGFQIRDLKMVTATEEL
                     ISQHYEQLTDKPFFPQLVQYMTSGPMIAGIIEGPEIIKSWRDMMGATNPVNALPGTIR
                     GDFATAPVEGIVANVVHGSDSAEAAEREIGLWLGK"
     gene            895..1566
                     /locus_tag="AC1PIW_001903"
     CDS             895..1566
                     /locus_tag="AC1PIW_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HIP6606793.1"
                     /translation="MGQGTTISLIKEEIIQQEKQIEGIFLEIENLRIMKKQCKNWLFF
                     AITMLFFSVIVFKGMFLVIMVFLCFMYVVTSYFQSDRCDGLISHYKNEIDSIEEAINK
                     NREFIAKYKYFSHFYVAGTQYREDRFEPMRVLRCLTYGGETTDVKLVREPDNKYDPNA
                     VKVLVCGYFVGYIPKTASEEVSRLIDRGEKLNLSVDMERQGSYDKGYRAYYELTIYVL
                     NDEKL"
BASE COUNT          535 a          234 c          368 g          477 t
ORIGIN      
        1 tccaggttat ggctatgcta aagtatccaa aacggagcgc gctaagtggg gcaaaatgat
       61 tgaagagtat ttgacgacac gagaaaatct acgtgttgtg gtgagtttag tagacatgcg
      121 tcatgagcca tcagcagatg atgttcaaat gtatgatttc ttgaaatatt atgaaattcc
      181 tgttattatt gtggcaacga aggctgataa aatcccacga ggaaagtgga ataagcacga
      241 atcgatgatt aaaaagaaat tggattttga taaaaatgat caattcgtta tcttttcttc
      301 tgagactcgt cacggatacg atcaggcatg ggatgctatc ttaagtaaca tctaataaac
      361 agttcaggag aaaaatcatg gaacgtactt tcttcatcat caaaccagat gctcttaagc
      421 gtggacttgc aggacaaatt ctttcacgta tcgaacgcag gggatttcaa atccgagatt
      481 taaaaatggt tacagcgaca gaagagctaa tcagtcaaca ttatgaacag ttgacagata
      541 aacctttctt cccgcaatta gtacaatata tgacgagcgg gccgatgata gctggcatta
      601 tagaagggcc agaaattatc aaatcttggc gagatatgat gggagctacc aatccagtaa
      661 acgccctacc tgggactatt cgtggagatt ttgcgacagc gcccgttgag ggaattgtgg
      721 caaatgtcgt tcatggctca gatagtgcag aagcagccga acgcgagatc ggtctttggc
      781 tgggaaaata gcagagcaga cacctagttt tctaggtgtt tttctttgat aaattggtga
      841 aaaaactgat tagtggtata atgaaaaaaa gcaataatag agaggttatt caccatgggg
      901 caaggaacaa cgattagtct tatcaaagaa gaaattattc aacaggaaaa gcaaattgaa
      961 ggtatatttt tggagattga gaacttgcgg attatgaaga agcagtgtaa aaattggttg
     1021 ttttttgcta taacgatgct gttcttctct gtcattgtat tcaaagggat gtttcttgtt
     1081 attatggtat ttctttgttt catgtatgta gtaacatcat atttccaatc ggatagatgt
     1141 gatggattaa ttagccatta taaaaatgag attgattcta tagaggaggc tatcaataaa
     1201 aatagagaat tcattgctaa gtacaaatat ttttcgcatt tttatgtagc aggtacgcaa
     1261 tatagggaag atcgttttga gccaatgagg gtgttaagat gtctgacgta cggcggagaa
     1321 actacagatg tcaagttagt tcgtgagcca gataataagt atgacccaaa tgcagttaaa
     1381 gtacttgtat gtggttattt tgttggctat atccctaaaa ctgcttctga ggaagtttcc
     1441 cgactaatcg atagagggga aaagttgaac ttgtcagtag atatggaaag acaagggagt
     1501 tatgataaag gttatcgtgc ttattacgaa ctgacaattt acgttttaaa tgatgaaaaa
     1561 ttgtaattat taaattcatt aaaaatatat cgagtttatg tgggacaagt aata
//
LOCUS       DBNFCK010000193         1613 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.195, whole genome shotgun sequence.
ACCESSION   DBNFCK010000193 DBNFCK010000000
VERSION     DBNFCK010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.195"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(74..910)
                     /gene="mreC"
                     /locus_tag="AC1PIW_001904"
     CDS             complement(74..910)
                     /gene="mreC"
                     /locus_tag="AC1PIW_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIP6606794.1"
                     /translation="MNKFSKLVVVVSIFLLLSFSLLFVTFSKGLQVPYLNNIVRVVVT
                     PIQSVISVPTRFFSEQKDVLTDLMNAYEENKQLKETIMSLEGMAAENTSLKEENASLR
                     SSLGVVSDFPEKQLIPGSVLVRTPSSWSEHISINIGETSGVTYNALVVANGGLVGIVS
                     SLSSDSAVVTLFTNSDEFTKLPVKISVDSKEIYGILSGYDADTNSFIINQLNSADEIA
                     VGSNVVTSDLAGETPANVQIGKVLSVKSNSNSLNREVYVEPTASFSNIYSVLVVGQTN
                     AQ"
     gene            complement(970..1057)
                     /locus_tag="AC1PIW_001905"
     tRNA            complement(970..1057)
                     /locus_tag="AC1PIW_001905"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1021..1023),aa:Ser,seq:gct)
     gene            complement(1070..1143)
                     /locus_tag="AC1PIW_001906"
     tRNA            complement(1070..1143)
                     /locus_tag="AC1PIW_001906"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1107..1109),aa:Ile,seq:gat)
     gene            complement(1175..1247)
                     /locus_tag="AC1PIW_001907"
     tRNA            complement(1175..1247)
                     /locus_tag="AC1PIW_001907"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1212..1214),aa:Phe,seq:gaa)
     gene            complement(1263..1336)
                     /locus_tag="AC1PIW_001908"
     tRNA            complement(1263..1336)
                     /locus_tag="AC1PIW_001908"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1300..1302),aa:Met,seq:cat)
     gene            complement(1347..1436)
                     /locus_tag="AC1PIW_001909"
     tRNA            complement(1347..1436)
                     /locus_tag="AC1PIW_001909"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1398..1400),aa:Ser,seq:tga)
     gene            complement(1443..1516)
                     /locus_tag="AC1PIW_001910"
     tRNA            complement(1443..1516)
                     /locus_tag="AC1PIW_001910"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1480..1482),aa:Ile,seq:cat)
     gene            complement(1533..1606)
                     /locus_tag="AC1PIW_001911"
     tRNA            complement(1533..1606)
                     /locus_tag="AC1PIW_001911"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1570..1572),aa:Met,seq:cat)
BASE COUNT          530 a          388 c          281 g          414 t
ORIGIN      
        1 taccaacgtt gaaatctgtc catcaagtaa taaaataaag aacaagatgg gaaacatgaa
       61 tatttctatc attttattgc gcatttgttt gacctaccac taaaaccgaa taaatgtttg
      121 aaaaactagc tgtcggctca acatatactt ctctatttaa actgttacta tttgatttaa
      181 ccgataaaac tttaccaatt tggacatttg ccggagtctc accagccaaa tcactcgtta
      241 caacattgct acccactgca atttcatcag ctgagttcaa ctgattaatg ataaaactat
      301 ttgtatctgc atcatagccc gataaaatac cataaatttc tttagaatca acagaaattt
      361 tcacaggtaa cttcgtgaat tcatccgaat tcgtaaataa ggtaacaacc gccgaatctg
      421 aactcaatga actcacgatg ccaactaatc caccattagc aaccacaagt gcattatatg
      481 tcacaccact agtctcacca atattaattg aaatatgctc cgaccacgat gaaggagtcc
      541 tcactaaaac tgatcccgga ataagttgtt tttcaggaaa atcagatacc acacctaaac
      601 tgctacgaag cgatgcattc tcttctttca agctagtatt ttcagcagcc atcccttcaa
      661 ggctcataat agtttccttt aattgtttat tttcctcgta agcgttcatc aaatctgtca
      721 agacatcttt ctgctcagaa aaaaatctag taggtactga aataaccgat tgaattggcg
      781 ttacaacaac cctaacgata ttatttaaat atggtacctg taacccctta gagaaagtca
      841 caaacaaaag agaaaatgac agtaataaaa agatagaaac aactactact aattttgaaa
      901 atttattcat aatatatcct caccaagata caataaaacg agaatatcaa gcgataatct
      961 cgttcactac ggagaataag ggattcgaac ccttgcgcca gttacccgac ctaacgattt
     1021 agcaaaccgt cctcttcagc ctcttgagta attctccatc aatataatat gggcacgagt
     1081 ggaatcgaac caccgacctc acgcttatca ggcgtgcgct ctaaccatct gagctacgcg
     1141 cccaagataa taaacttgga caaagttaaa ttaatggcgc gagacggaat cgaaccgccg
     1201 acacatggag cttcaatcca ttgctctacc aactgagcta ccgagccaaa ttcttaaaga
     1261 aattgcggga gcaggatttg aacctacgac cttcgggtta tgagcccgac gagctaccta
     1321 gctgctccat cccgcggtag tagaaaagga ggatgtggga ttcgaaccca cgcacgcttt
     1381 tacacgcctg acggttttca agaccgttcc cttcagccgg acttgggtaa tcctccgata
     1441 tatggacctt gtaggacttg aacctacgac cgctcggtta tgagccgaga gctctaacca
     1501 gctgagctaa aggtccagca aacaagagta tatagcggcg aaggggatcg aacccccgac
     1561 ctcccgggta tgaaccggac gctctagcca gctgagctac accgccaaaa taa
//
LOCUS       DBNFCK010000194         1603 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.196, whole genome shotgun sequence.
ACCESSION   DBNFCK010000194 DBNFCK010000000
VERSION     DBNFCK010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.196"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            339..494
                     /locus_tag="AC1PIW_001912"
     CDS             339..494
                     /locus_tag="AC1PIW_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606795.1"
                     /translation="MNHPKFTLRKLSIGLVSLGAGVALSGTLYPQNTVTVYAETPVTE
                     STTLEKK"
     gene            563..>1603
                     /locus_tag="AC1PIW_001913"
     CDS             563..>1603
                     /locus_tag="AC1PIW_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIP6606796.1"
                     /translation="MKIRKERWLTPLLFSALPKSNAESASPEALTNENASEPTGQVAE
                     NTSSSEGKATEKEEPAVQYVEKEVDDYSTKVEKPNETVSSTVDKTPKELFTVSRTAEV
                     GQDGVVTVTTQIVPKEIDKGAEIVVLVDTSKKMDEEAKKAAKDNIVKLVKEMTDPTNN
                     HNSRNSVRVIGFNRKLSEAQEVNSITVEDTVEKLFTDAEQNYNWGVDMQGAIHEARRI
                     LESEKSGKRKHIVLLSQGESTFSYDLTDAAKTETKYKTINEDKVVHTNPLLPWPFTFD
                     VTVRRANLLKDAKSLIEFLNKLGITRFNEALDGVSTVAGLTDFIGKFFRNPLDYIDLA
                     DIDSSKLEEQKFN"
BASE COUNT          567 a          262 c          334 g          440 t
ORIGIN      
        1 agaaaaaaca aattctgttt ttaaattata tggtagaatg aattatgtag taatagactc
       61 gcacattttg tgatatggtc attgttaatg ataaatagga actttgagtt gtaatcatgc
      121 ttgcttgact tgataggtga agtaagcagt taacagaagg acttaggcac cttatgaata
      181 cacaaggtgc ccattctttt cccaaaagct ctacgcttct tgaacaaaat gatactaaag
      241 ttatttacca ttgacatcca tacttccttt tttgaaaata cctaaagaag ggcgaaaata
      301 cattctaaat ttatagagaa caaggagaag gaatcataat gaatcatcca aaattcactt
      361 tgcggaagct atcaattgga cttgtttcgc tgggagcggg ggtagcttta tcgggaacac
      421 tctatcctca aaatacagtg acagtatatg cagaaacgcc tgttacagaa agtactactt
      481 tagaaaaaaa atgaggagat agataatact tcttcgatta cacgtcctat tgctgttgaa
      541 gaccctaaag ttgtccaagc taatgaaaat aaggaaggag aggtggttga ctcctcttct
      601 attttctgcc ttaccaaaat cgaatgcaga atctgcttct cctgaggcat taacgaatga
      661 aaacgcttca gagccaacgg ggcaagttgc tgaaaacact agttcttctg aaggaaaagc
      721 aacagaaaaa gaagaaccag ccgttcagta tgtggagaag gaagtagatg actattctac
      781 taaggttgaa aaaccaaatg aaacagtatc tagtacagtg gataaaactc ctaaagagct
      841 ttttacagtt tctcgaacag cagaagtagg gcaagatgga gttgtcacag taacaactca
      901 aattgtgccg aaagaaattg ataagggtgc agaaatagtt gtcttggtgg atacatctaa
      961 aaaaatggat gaagaggcta aaaaagcagc taaagataac attgtcaagc ttgtcaaaga
     1021 aatgactgac ccaaccaata atcataattc tcgtaactct gtgcgtgtga tcggtttcaa
     1081 tcgcaagtta tctgaagctc aagaggttaa cagtattact gtagaagaca cggttgagaa
     1141 gcttttcact gatgctgaac aaaattacaa ttggggcgta gatatgcagg gagcaatcca
     1201 tgaagcacgt cgcattttag aaagcgagaa atcagggaaa cgcaaacata ttgtacttct
     1261 gtcacaaggg gagtctacat ttagttatga tttgacggat gctgcgaaaa cagaaactaa
     1321 gtataagaca atcaatgaag ataaagtggt gcatactaac cctctattgc cttggccatt
     1381 tacatttgat gtgactgtcc gcagagcaaa tttgttaaaa gatgccaaga gcttgattga
     1441 attcttgaat aaattgggaa ttacaaggtt taatgaggca ttagatggag tatctactgt
     1501 cgcaggttta actgacttta ttggaaagtt tttcagaaac ccgttagact atattgactt
     1561 agctgatatt gatagctcca aattagaaga acaaaaattc aat
//
LOCUS       DBNFCK010000195         1594 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.197, whole genome shotgun sequence.
ACCESSION   DBNFCK010000195 DBNFCK010000000
VERSION     DBNFCK010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.197"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..1097
                     /locus_tag="AC1PIW_001914"
     CDS             <1..1097
                     /locus_tag="AC1PIW_001914"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HIP6606797.1"
                     /translation="GTIGDVGIVPENFNNALLTENALKLMLTESINKMFLAHLLKSPL
                     VQKQFKEVYNQVAQPKLSIRSTNSTIIPLPPLAEQKRIVAQIERALEQVEVYAESYNK
                     LQELDRAFPDKLKKSILQYAMQGKLVAQDPNDEPVEVLLEMIRAEKQKLYEEGKLKKK
                     DLAEIMVEKGDDNSPYGKNKENIGFSNSTLFKLPSSWCYVKFGGLVLFNIGKTPPRSE
                     PNYWGDDIPWVSISDMSNNGHIFKTKEYLSDFAINQKKVKIASAGTLLMSFKLTIGKV
                     ALEVPASHNEAIISIFPYGDKENIIRDYLMRFLPLISTTGNSKDAIKGKTLNSTSISG
                     LLIPISSYREMKDIVTKVDLLFEKVAQLYY"
     gene            complement(1051..>1594)
                     /locus_tag="AC1PIW_001915"
     CDS             complement(1051..>1594)
                     /locus_tag="AC1PIW_001915"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HIP6606798.1"
                     /translation="PRNWTLLSVKDIFSITTGLSYKKTDLAIIQRGVRIIRGGNIEPL
                     AYKLLDNDYYIESKYITSESVYLKRNQLVTPVSSSLEHIGKFARIDKNYSDTVAGGFV
                     FQLTPFISSDTLSNYLLLCLSSPLFYKQLQSVTKLSGQALYNIPKTKLNDLRIALAPE
                     QEQERISNKVGQLFQKVNLL"
BASE COUNT          543 a          264 c          297 g          490 t
ORIGIN      
        1 ctggaactat tggtgatgtt ggaatagttc cggaaaattt taataatgct ctattaaccg
       61 aaaatgccct taaactgatg ctgacagaaa gtatcaataa aatgtttttg gctcatctct
      121 tgaagtctcc attagttcaa aaacaattca aagaagttta taatcaagtt gctcagccta
      181 aactgtccat tcgtagcaca aactcaacaa ttatccctct cccaccccta gccgaacaaa
      241 aacgaattgt tgcccaaatt gagcgagcct tggaacaagt tgaagtctat gctgaaagtt
      301 acaataaact ccaagagtta gatagagctt ttccagataa actgaaaaaa tctatcctcc
      361 agtatgctat gcaaggaaaa ttagttgcac aagatccgaa cgacgaaccc gtcgaagtct
      421 tacttgaaat gattcgtgct gaaaagcaaa aactctatga agaaggcaag ctcaagaaga
      481 aagatttggc ggaaataatg gtcgaaaaag gagatgataa ctctccttat gggaagaaca
      541 aggaaaatat tggttttagt aattcaactt tgtttaaact tcctagttct tggtgctacg
      601 ttaagtttgg tggtctagtt ttatttaata ttggaaaaac acctccaaga agtgagccga
      661 attattgggg agacgatatt ccttgggtat ctatttcaga tatgtctaac aatggtcaca
      721 tcttcaaaac aaaagaatat ctatcagatt ttgctattaa tcagaagaag gtaaagatag
      781 cttcggcagg caccttgttg atgagtttta agttaactat tggtaaagta gcattagagg
      841 tccctgctag ccataatgaa gcgataatct caatatttcc gtacggagat aaagaaaata
      901 tcataagaga ctatctgatg cgatttttgc ccctaatctc aaccactggt aattcaaagg
      961 atgctataaa aggaaaaacg ttaaatagta ctagtatttc aggattattg atacctattt
     1021 ctagctatcg agaaatgaaa gatattgtaa ctaaagtaga tttacttttt gaaaaagttg
     1081 cccaacttta ttactgatac gttcttgctc ttgttcagga gctaaggcaa tacggagatc
     1141 attcaacttt gtttttggaa tattatataa agcctgtcca gacagcttgg ttactgattg
     1201 tagttgtttg taaaaaagtg gcgatgagag acaaagtaat agatagtttg ataaagtatc
     1261 tgacgagata aatggtgtta gttgaaatac aaatcctcca gctactgtat ctgaataatt
     1321 tttatcaatt ctggcgaatt ttccgatatg ttctaacgat gaagagaccg gggttactag
     1381 ttgatttcgc ttcaagtaaa cagattctga tgtaatatat tttgactcaa tatagtagtc
     1441 attatctaat aatttatatg ctaagggttc gatatttccc ccacgaataa tccgcacacc
     1501 tctttggatg atagctaaat ctgttttctt ataagataac cctgtcgtaa ttgaaaaaat
     1561 atctttgaca gaaagaagag tccaatttct cgga
//
LOCUS       DBNFCK010000196         1524 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.198, whole genome shotgun sequence.
ACCESSION   DBNFCK010000196 DBNFCK010000000
VERSION     DBNFCK010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.198"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..1314
                     /locus_tag="AC1PIW_001916"
     CDS             <1..1314
                     /locus_tag="AC1PIW_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIP6606799.1"
                     /translation="IYLQLLILFLLTLFNAFFSASEMALVSLNRSRVEQKAADGERKY
                     IRLLQVLENPNNFLSTIQVGITFISILSGASLANDLGAVFAKWMGNSTTAQTAGYWLA
                     LAMLTFVSIVLGELYPKRIAMNMKENLAVVTAPVIIFLGKIVSPFVWLLSAATNLISR
                     ITPMNFDDADEQMTRDEIEYILTKSEKTLDAEEIEMLQGVFSLDELMAREVMVPRTDA
                     FMVDIEDDTATIMAAILKQNFSRIPVYDGDKDNIIGLIHTKKILSEAFSNGFDNLNIR
                     RIMQEPLFVPETIFVDDLLTSLRNTQNQMAILLDEYGGVAGLVTLEDLLEEIVGEIDD
                     ETDKTEIFVREIADNTYIVQGNMTLNDFNEHFEMELESDNVDTIAGYYLTGVGTIPSQ
                     DEKVSFEVDSKDHHLVLINDKVKNGRVTKLKLLITPLEKEETDKD"
     gene            1438..>1524
                     /gene="pflA"
                     /locus_tag="AC1PIW_001917"
     CDS             1438..>1524
                     /gene="pflA"
                     /locus_tag="AC1PIW_001917"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645131.1"
                     /note="activates pyruvate formate-lyase 1 under anaerobic
                     conditions; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HIP6606800.1"
                     /translation="MTEIDYRKVTGLVHSTESFGSVDGPGVRF"
BASE COUNT          454 a          262 c          341 g          467 t
ORIGIN      
        1 atctatttac aactgttaat tttattctta ttgactctgt tcaatgcctt cttttcagcc
       61 tcagaaatgg cccttgtatc cctcaatcgt tcaagagtag aacaaaaggc agcagacggt
      121 gagaggaaat atattcgctt gcttcaagtt ttagagaatc ctaacaattt tctatcaacc
      181 attcaggtcg gtattacctt catctccatc ctttcaggtg ccagcttggc taatgattta
      241 ggagctgtat ttgccaaatg gatggggaat tcaacaacag ctcaaactgc tggttactgg
      301 cttgcactgg ctatgttgac cttcgtttca atcgttcttg gcgaattgta tcctaagcgg
      361 attgccatga atatgaagga aaatctggca gtagtcactg caccagtgat tattttcctt
      421 ggaaaaattg ttagtccgtt cgtgtggtta ttatcagctg caacgaatct tatcagccgt
      481 attactccta tgaattttga tgatgcggat gagcaaatga cacgggatga aattgaatat
      541 attttgacaa agagtgagaa aaccttagat gccgaagaaa tcgaaatgct tcaaggtgtg
      601 ttctctttag atgagctgat ggcacgtgaa gtaatggttc cccgtacgga tgctttcatg
      661 gtggatattg aggatgatac agcaaccatc atggcagcta tcctcaagca gaatttttca
      721 cgtatccctg tttatgatgg cgataaggac aatatcattg gtctgattca taccaagaaa
      781 atcttgtcag aagctttcag taatggcttt gacaatctca acatccgccg cattatgcag
      841 gagcctctct tcgttccaga aacgattttc gtggatgatt tgttgacatc attgagaaat
      901 acccaaaatc aaatggccat cttgttagat gagtatggtg gagttgcagg tctcgttacc
      961 ttggaagatt tgctggaaga aattgttggg gaaatcgatg atgagaccga taagacagaa
     1021 atttttgttc gagaaattgc tgacaacacc tatatcgtcc aaggaaatat gaccttgaat
     1081 gacttcaatg agcattttga gatggagttg gaaagtgata acgttgatac gattgctggt
     1141 tattatctga caggagtcgg taccattccg agccaagatg agaaagtttc ctttgaagta
     1201 gatagtaaag atcaccacct agttcttatt aacgataagg taaaaaatgg tcgcgtaaca
     1261 aaattaaaac tcttgattac accactggag aaagaagaaa ccgataagga ctagggcaac
     1321 ctagtctttt agcttttttc ttgcggaaga tgatggttat gaaagaaaga aagtaaatga
     1381 tagaagcgtt ttcatttttg tgatatacta gtcttataaa atgatgagga tgagatgatg
     1441 acagaaattg attatcgtaa agtgacaggt ttggttcatt caacagagag ttttggttcg
     1501 gttgatggcc caggggtacg tttt
//
LOCUS       DBNFCK010000197         1507 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.199, whole genome shotgun sequence.
ACCESSION   DBNFCK010000197 DBNFCK010000000
VERSION     DBNFCK010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.199"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..427)
                     /locus_tag="AC1PIW_001918"
     CDS             complement(<1..427)
                     /locus_tag="AC1PIW_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HIP6606801.1"
                     /translation="MKSRIVSPKIIFIWNLLGSVSSAAISIFLLLLVTRLLTELEADI
                     FSFAYAVANLFVIIASFQVRDYQATDVSKKFSFSQYLATRLITITIMLLLALSYIFLS
                     QYEFQKSACIFLICLYRGSDALSDVFQGLFQQNARLDIAG"
     gene            complement(460..>1507)
                     /locus_tag="AC1PIW_001919"
     CDS             complement(460..>1507)
                     /locus_tag="AC1PIW_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhamnan synthesis F family protein"
                     /protein_id="HIP6606802.1"
                     /translation="FTEYPDRIYSLLHLIRMKTKYPVELIERHLRQIIIPGTSFIPQI
                     RVSQTTETVRSSTSVLLHVHIESVSIFEEYIEELCKIADRCQLLITLPEADFSNKCSI
                     VERCLFTYQLRAQIAKLTDELHFFEIVNNYMGDAKYLAHVTVKQTKEIKYSVEDIIDR
                     HQLRKMFFTSFDAVISNFESQSNLAVVIPDLTTNQRYDRQSLREGNPELIRQLNILYE
                     SLVRTKKVDFYKVPYIIGEEVSWYWIKTEHYKKIEEKFRNIDFSKEDRLLLVPILFIY
                     SAWDLSNDYAVVENTENVSPILEKISFSSERELRLIIEEKEFLQIGLRRTLKIISVGI
                     VSVFKMIKKSLLKN"
BASE COUNT          499 a          286 c          200 g          522 t
ORIGIN      
        1 tacctgcaat atctagccga gcattttgtt ggaacaatcc ttgaaaaaca tccgatagag
       61 catcacttcc acgatacaaa catattagga aaatacaagc agatttttga aattcatatt
      121 gagacagaaa aatatagctt aaggcaagta atagcataat agtgattgtt ataagccttg
      181 ttgcaagata ctggctaaac gaaaattttt tcgaaacatc agtagcttga taatctctta
      241 cctgaaaact agcaatgatt acaaaaagat ttgctactgc ataggcaaaa ctaaaaatat
      301 cagcctctaa ttccgtcaac aaacgtgtta ctagtaacaa taaaaatatt gatattgctg
      361 ctgaggaaac acttcctaat aaattccaaa taaatattat ttttggtgat actattctgc
      421 tcttcacact ttcttcccct tgtttgtcat ccgttatccc taatttttta atagagattt
      481 ctttatcatc ttaaacacgc ttacaattcc aactgatatt atctttagtg tccttctcag
      541 tccaatttgt aaaaattcct tctcttcaat aattaaacgc aattctcttt cactagaaaa
      601 actaatcttt tccaatattg gactcacatt ttcagtattc tcgaccactg cataatcgtt
      661 tgataaatcc cacgcgctat aaataaataa tattggaact agcaataatc tatcttcttt
      721 tgaaaaatca atgtttctaa atttctcctc aatcttttta tagtgttctg tttttatcca
      781 ataccaactt acttcttctc caattatata aggcacttta taaaaatcaa ctttcttagt
      841 tctcacaagt gattcataaa gtatattcaa ttgacgaata agttctggat tgccctctcg
      901 aagactttgt ctatcatatc tttgattagt tgttaagtct ggaataacta cagccaaatt
      961 agattgtgat tcaaaattag aaatcactgc atcaaatgat gtaaaaaaca ttttcctcaa
     1021 ctgatgcctg tcaataatat cctctacaga atattttatt tcctttgttt gtttgacagt
     1081 tacatgagca agatatttgg catctcccat atagttgttt actatttcaa aaaaatgcaa
     1141 ttcatctgtg agttttgcaa tctgtgctct caattggtag gtgaataaac atctctcaac
     1201 tatactacac ttatttgaaa aatcagcttc tggcaatgtt attagtaact ggcacctatc
     1261 ggctattttg cataactctt caatatactc ttcaaaaata gacactgatt ctatgtgaac
     1321 atgaagaagt accgacgtcg aagaacgaac ggtctctgtc gtttgcgaaa cccgtatttg
     1381 aggaataaaa ctcgtacccg gaatgattat ctgcctcaaa tgcctctcga ttaattcaac
     1441 tggatatttc gttttcatcc taataagatg taataaagag tatattctgt ctggatattc
     1501 ggtaaaa
//
LOCUS       DBNFCK010000198         1439 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.200, whole genome shotgun sequence.
ACCESSION   DBNFCK010000198 DBNFCK010000000
VERSION     DBNFCK010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.200"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            2..703
                     /locus_tag="AC1PIW_001920"
     CDS             2..703
                     /locus_tag="AC1PIW_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000452012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6606803.1"
                     /translation="MEVIIQNLRKSYKDKEVLKDVSFKIEEGTICGLLGVNGAGKSTI
                     MKILFGLERADSGEVIFNGKHKDIVEENRFDIGALIESPAIYLNLSAFDNLKTRALLH
                     DIPDERIYEVLQLVGLSDTGKKKAGHFSLGMKQRLGLGMAIITNPDLLILDEPTNGLD
                     PDGIKELLNLIRDLKKSGMTVLLSSHQLSEVSKIADQIVILNQGKIFYDDINKNSNDL
                     EKLFFRIVHGGDGND"
     gene            696..1433
                     /locus_tag="AC1PIW_001921"
     CDS             696..1433
                     /locus_tag="AC1PIW_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013851902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic ABC transporter permease"
                     /protein_id="HIP6606804.1"
                     /translation="MTNIFKSEWLKQKSSSDKKLLILIPLLAIFIAFILAGPLNLESF
                     SIYWWEGGFLFTLLGLLFLADYSAEENAGAFQNISLGRYTFTIYLAKILLKFKDVLIS
                     TFIFTIIFYGISFLFSGVISVDIGKDLICLLLIGVSSAWVLPLLYFLAKWINPYLLLA
                     TNSLICLLVAPLIAQTSVWFIFPYTYHYKIAYALLHLKPSGDLEAALSGVEISTILIS
                     VSLSILVFVSFLSLLKWRITNEQNSKK"
BASE COUNT          452 a          193 c          278 g          516 t
ORIGIN      
        1 aatggaagtt atcattcaaa atctaagaaa aagctataaa gacaaagagg ttttaaaaga
       61 cgtttcattt aaaattgaag aaggtactat ttgtggtctt ctgggagtaa atggggctgg
      121 gaaatccacc attatgaaaa ttctttttgg gttagaaaga gctgatagtg gagaagtcat
      181 tttcaatgga aagcataagg atattgtaga agaaaataga tttgatattg gagcgttgat
      241 agaatctcct gctatctatt tgaatctttc tgcatttgac aatttaaaaa cacgagcatt
      301 gctacatgat attccagatg aaagaattta tgaggtactt cagttggttg gtttatctga
      361 tactggaaaa aagaaggcag ggcacttttc attaggaatg aagcaacgac ttggtttagg
      421 aatggcaatt attaccaatc ctgatttatt aattttagat gagccaacta atggtctaga
      481 tccagatggg ataaaagaat tgctaaattt aattagggat ctaaaaaaat ctggaatgac
      541 agtcctcttg tccagccatc aactaagtga agtcagcaaa attgcggatc aaatcgttat
      601 actgaatcag ggcaagattt tttacgatga tattaataag aattcaaatg atttagaaaa
      661 actattcttt agaattgtcc atggaggtga tggaaatgac taatattttt aaatctgagt
      721 ggctgaagca aaaatctagt tctgataaaa aattactaat ccttattcct ttgctagcaa
      781 tttttatagc tttcatacta gcaggacctc tcaatttaga aagtttttca atttattggt
      841 gggagggagg attccttttc actctccttg ggttattgtt tttagctgat tatagcgcgg
      901 aggagaatgc aggtgcgttt caaaatatta gcttgggtag atatacgttt actatttatt
      961 tagcgaagat actgttgaag ttcaaggatg ttttgatttc aacctttatt tttacgataa
     1021 tattttatgg catctcattt cttttttcag gtgtgatatc agtagacatt ggtaaagatt
     1081 tgatttgttt attacttatt ggtgtttctt ctgcttgggt attaccacta ttatattttc
     1141 tagcaaagtg gataaatccc tacttgttgt tggctacaaa ttctcttatt tgcttattag
     1201 ttgctcctct aatagctcag acatcggttt ggttcatatt tccttatact tatcactata
     1261 aaattgctta tgctctttta catttaaaac cctcaggtga tttagaggca gcattaagtg
     1321 gagttgaaat atcgacaatt ttaatatcag tatctctttc aatcttagtt tttgtgagtt
     1381 tccttagttt gttgaagtgg aggataacta atgaacaaaa ttctaaaaag tgagttgct
//
LOCUS       DBNFCK010000199         1426 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.201, whole genome shotgun sequence.
ACCESSION   DBNFCK010000199 DBNFCK010000000
VERSION     DBNFCK010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.201"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            60..539
                     /gene="rlmH"
                     /locus_tag="AC1PIW_001922"
     CDS             60..539
                     /gene="rlmH"
                     /locus_tag="AC1PIW_001922"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HIP6606805.1"
                     /translation="MKIKLITVGKLKEKYLKEGIAEYSKRLGRFTKLDMIELPDEKTP
                     DKASQAENEQILKKEADRIMSKIGERDFVIALAIEGKQFPSEEFSQRISDIAVNGYSD
                     ITFIIGGSLGLDSCIKKRANLLMSFGQLTLPHQLMKLVLIEQIYRAFMIQQGSPYHK"
     gene            564..1247
                     /locus_tag="AC1PIW_001923"
     CDS             564..1247
                     /locus_tag="AC1PIW_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HIP6606806.1"
                     /translation="MNQKEYRVFEGLRIACSLTFISGYLNAFTFVTQGGRFAGVQSGN
                     VISLAYFLAKGDFAQVVNFSIPILFFVFGQFFTYLARRYFEKQTWSWHFGSSVMMLVL
                     ILLTIILSPIMPASFTIASLAFVASIQVETFRRLRGAPYANVMMTGNVKNAAYLWFKG
                     VIEKDSELRKTGRNILLTIIGFMLGVIISTHLSFQFEEYALIGLILPVLYINYELWQE
                     KRPTRGRSK"
     gene            complement(1245..1318)
                     /locus_tag="AC1PIW_001924"
     tRNA            complement(1245..1318)
                     /locus_tag="AC1PIW_001924"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1282..1284),aa:Arg,seq:tct)
BASE COUNT          460 a          218 c          274 g          474 t
ORIGIN      
        1 atcctgattt tctaaaaata ttttctgtcg tatgcttgtg gatatgttac aattatttta
       61 tgaaaataaa attgattacc gttggaaaat tgaaagaaaa gtacctcaaa gaaggtattg
      121 cagaatatag taaacgattg ggacgattta ctaagttgga tatgattgag cttcctgatg
      181 aaaaaacacc agataaagcc agtcaggcag agaatgaaca aatattaaaa aaagaagccg
      241 atagaattat gtctaaaatt ggagagcgag attttgtcat tgccttagcg atagaaggga
      301 aacaatttcc atcggaagaa tttagtcaaa ggatatctga cattgcagta aatgggtatt
      361 cagatataac ttttatcatc ggtggtagtt tgggtctcga ttcttgtatt aaaaaaagag
      421 ctaatttgtt gatgagtttt ggacagttga cacttcccca tcaactaatg aaattagttc
      481 tcatcgagca gatttatcgt gcatttatga ttcagcaggg aagcccatat cataagtagt
      541 ttcacgtgaa acaaggagtg aaaatgaatc aaaaagagta tcgtgttttt gagggattga
      601 gaattgcttg ttcattaacg tttatcagtg gttatttaaa tgcctttact tttgtgactc
      661 agggtggtcg ctttgctggc gtacaatctg gaaatgttat ttccctagct tattttttag
      721 ctaaaggtga ttttgcgcag gtagttaatt tttccattcc cattttattt tttgtattcg
      781 gacaattttt tacctactta gcaagaaggt attttgaaaa acaaacatgg tcttggcact
      841 ttggtagtag tgtaatgatg ttagttctta ttttactaac tatcattctc tcacctataa
      901 tgcctgcgtc ttttacaatt gctagtctag ccttcgtagc ctctattcaa gtagaaacat
      961 ttagaaggtt acgaggtgct ccgtatgcca atgtgatgat gacagggaat gtcaaaaatg
     1021 ctgcttatct ctggtttaaa ggagttattg aaaaagattc agaacttaga aaaacaggta
     1081 gaaacatctt attgaccatt atagggttta tgctaggtgt catcatatct actcacctat
     1141 ccttccaatt tgaagaatat gcccttattg gtctgatttt gccagtgtta tatattaatt
     1201 atgaattatg gcaagaaaaa agacctactc gaggtaggtc taaatgattc cagcaggatt
     1261 cgaacctgcg accgttcgct tagaaggcga atgctctatc cagctgagct atggaacctt
     1321 aaccatttta ttttacatta aacaaagcac tctgtcaata caataaaaaa tcaaaaaatg
     1381 tattgacaaa caaagtcaca tgtattatac tagttattgt tctaaa
//
LOCUS       DBNFCK010000200         1400 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.202, whole genome shotgun sequence.
ACCESSION   DBNFCK010000200 DBNFCK010000000
VERSION     DBNFCK010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.202"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            131..1135
                     /gene="ccpA"
                     /locus_tag="AC1PIW_001925"
     CDS             131..1135
                     /gene="ccpA"
                     /locus_tag="AC1PIW_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HIP6606807.1"
                     /translation="MLNTDDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLE
                     VIDRLDYRPNAVARGLASKKTTTVGVVIPNIANAYFATLAKGIDDIADMYKYNIVLAN
                     SDENDEKEINVVNTLFSKQVDGIIFMGYHLTDKIRAEFSRSRTPIVLAGTVDLEHQLP
                     SVNIDYAAASVDAVNLLAKNNKKIAFVSGPLVDDINGKVRFAGYKQGLKDNGIEFNEG
                     LVFESKYKYEEGYALAERILNAGATAAYVAEDEIAAGLLNGVSDMGIKVPEDFEIITS
                     DDSLVTKFTRPNLTSINQPLYDIGAIAMRMLTKIMHKEELENREVVLNHGIKVRKSTK
                     "
BASE COUNT          455 a          262 c          304 g          379 t
ORIGIN      
        1 aaaaaattgc aagtattatc aaaaaaatag agtttatctc ttgaaagtgg tttcaaaaag
       61 tgatacacta tacttgagtg aaagcgtaat tttcatagaa ccagaactat tgaaagggag
      121 tacataacgt atgttaaaca ctgacgatac ggtaacgatt tatgacgttg cccgcgaagc
      181 aggtgtatcc atggcgacag tatcgcgcgt ggtaaatggg aataaaaacg taaaggaaaa
      241 tactcgtaaa aaagtattag aagtcatcga ccgcttggat tatcgtccga atgctgttgc
      301 acgtggattg gccagcaaga agactaccac tgtgggggtt gtgattccaa atattgctaa
      361 tgcttatttt gcaaccttgg ccaaaggtat tgatgatatt gccgatatgt acaaatacaa
      421 tatcgtccta gcaaacagtg atgaaaatga tgagaaagaa atcaatgtgg taaataccct
      481 attctcaaaa caggtggacg gaatcatttt catgggctat catttgacag acaagattcg
      541 tgcggagttt tcacgctcac gtacaccgat tgttttagct ggtaccgtgg acttggagca
      601 ccaattacct agcgtcaata ttgactacgc tgccgctagt gttgatgcag tcaatctatt
      661 agctaagaac aacaagaaaa ttgcctttgt atcagggccg cttgtagatg acatcaacgg
      721 taaagttcgt tttgcaggct acaaacaagg cttgaaggac aacggaatcg agtttaacga
      781 aggattggtt tttgaatcca agtataaata cgaggaaggc tacgctctag cagaacgtat
      841 tttgaatgcc ggagcaactg cagcttatgt tgcagaagat gagattgctg caggtctatt
      901 gaacggtgtc agtgatatgg gcatcaaggt tccggaagat tttgaaatca tcacaagtga
      961 tgattcccta gtgactaagt ttacccgtcc aaacctgacc tctatcaatc agccactata
     1021 tgatattggt gcaattgcta tgcgcatgct taccaaaatc atgcacaagg aagagttgga
     1081 aaaccgtgag gtagttctta accacggaat caaagtacgt aaatcaacta agtgataaat
     1141 atgggagtag ccggataaga tatcttatta ctccgccccc tcactctatc aaacattata
     1201 atcatttagt ttatctttta ttactttgac gagcgaggaa atatttccaa cctagaacgg
     1261 cctttaggct gttctagcaa cctgtagcta ttgaggcaaa cttgcttcga cttatctcca
     1321 gccttcaaag gttcggagaa cctttgaagc gagtactaaa agcaaacgaa atggctatac
     1381 taaccgaaaa ctaaaagatt
//
LOCUS       DBNFCK010000201         1371 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.203, whole genome shotgun sequence.
ACCESSION   DBNFCK010000201 DBNFCK010000000
VERSION     DBNFCK010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.203"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..792)
                     /locus_tag="AC1PIW_001926"
     CDS             complement(<1..792)
                     /locus_tag="AC1PIW_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606808.1"
                     /translation="MKRDKQVLRDKTNIIVDNLLPENKAYFDEIRDYMILKSFFKDEK
                     AILEQIYSMVSDFKMAEVDGLTAVDFFGTDPKDMADQLLKNAPKAPLREMTITYFSAV
                     ATLYAIGTFLLFANTGQFQLEFILFLASCTNALLLCWLVFGLLPSVLFKPKASKMIIG
                     LIVLTIIILFNIIFNLPAMIGDKAIYLKFPEMFDWLFVIVTSLATIVFSVKVKVFRTF
                     ALPIFAFLLIGIIKKLLVMNLVSGDLWTVWLPIGLLCAAMILFYLQ"
     gene            complement(779..1114)
                     /locus_tag="AC1PIW_001927"
     CDS             complement(779..1114)
                     /locus_tag="AC1PIW_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIP6606809.1"
                     /translation="MKETQMLKGVLDGCVLQIISQKEIYGYELVQELRKQGFENMVGG
                     TAYPLLQKLEKNGYIIGEIKPSSDGPDRKYFTITQDGESYLRDFWREWSSLVEKVGTI
                     QKGIFNETR"
     gene            complement(1111..1275)
                     /locus_tag="AC1PIW_001928"
     CDS             complement(1111..1275)
                     /locus_tag="AC1PIW_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606810.1"
                     /translation="MKLEKKSRENWKLAGITLVTAIITGWALDSILILGILLGSGLLF
                     GFFTDDEEGL"
BASE COUNT          443 a          302 c          205 g          421 t
ORIGIN      
        1 ctgtagatag aataaaatca tggcagcaca tagaagacca atcggtagcc aaactgtcca
       61 taaatcacca ctaaccagat tcatcaccaa taattttttg ataatgccta tcagtaaaaa
      121 agcaaaaatc ggtaaagcaa aggttctgaa tactttcacc ttaacagaga aaactatcgt
      181 ggccaaactt gtcacaatca caaataacca atcgaacatt tctggaaatt tcaaatagat
      241 tgccttatct ccaatcattg ctggtaaatt gaaaataatg ttaaaaagaa taattattgt
      301 taggacaatc aagcctataa tcattttaga agcctttggc ttaaaaagaa cagatggtaa
      361 cagaccgaaa accagccaac aaagcaacaa agcattggtg caagaagcca aaaacaggat
      421 gaattctaat tgaaattgtc ccgtgtttgc aaacaaaagg aaggtgccaa tggcgtacaa
      481 ggttgcgacc gcactgaaat aggttatcgt catttccctc aagggagcct tcggtgcatt
      541 ttttaaaagt tgatctgcca tatctttggg gtctgttcca aaaaaatcta ctgctgttag
      601 accatcaacc tcagccattt tgaaatcact gaccatacta taaatctgtt ccaaaatcgc
      661 tttttcatct ttaaaaaagg atttcaaaat catatagtcg cgtatctcat caaaataagc
      721 tttattttca ggtaacaaat tatctacaat tatatttgtc ttatctcgca agacttgttt
      781 atcgcgtttc attaaatatt cctttctgaa tggttcctac tttttctaca agacttgacc
      841 attcccgcca aaaatccctt aaataactct ctccatcttg ggtaatcgta aaatatttac
      901 gatctggccc gtccgatgat ggttttatct cgccaatgat ataaccattc ttttctaatt
      961 tttgcaagag aggataggca gttcccccga ccatgttttc aaagccctgt tttctcaatt
     1021 cctgaaccag ctcataacca taaatctcct tctgactgat aatttgtaag acacagccgt
     1081 ctaggacccc ctttagcatt tgggtttctt tcataagccc tcctcatcat ctgtaaaaaa
     1141 tccaaacagt aaacctgatc ctaacaggat gcctaagatc aaaatagaat ccaaggccca
     1201 gcctgttata atggccgtca caagcgtgat tcctgctagt ttccaatttt cccgactttt
     1261 cttctccagt ttcatactgc ctccctacct agtttgtaat accgactagt ttgataaaaa
     1321 taaaagtgat tttctttgtc ttctatcact agtttgtttt acctattagc t
//
LOCUS       DBNFCK010000202         1307 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.206, whole genome shotgun sequence.
ACCESSION   DBNFCK010000202 DBNFCK010000000
VERSION     DBNFCK010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.206"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..>1307
                     /locus_tag="AC1PIW_001929"
     CDS             <1..>1307
                     /locus_tag="AC1PIW_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001213956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIP6606811.1"
                     /translation="TKENTRFIGVKNINSVEGSKNRLLTDFNCEPNFLLDNDSHDIDS
                     VGKNNMWIKGGKISFKMFQEALLDYTVSVSLFEPNFEQKSYIKGLYIQSRGGDRSFLT
                     GDKLEKDRDFFLTFSPSMNCLIGGRGTGKSTLIDMLQFVLSQDCDKQSKLEFLCNHAN
                     AFVLYVLEDAEYIIEVSLPDVLQENRDNILQYYGQNRENRYGYPYNYNSDSIKEWTRS
                     QYTKVYKVEGKFFKLVDKTRILEKMFDRRYSVNELVRTADGEKITEFISDLMLKNKNL
                     PRPNYGLRTQTLESFEAKLQELDKYRRVRKDSILKIIDDFNQTQVGKLRICYEQIDRW
                     EVPDFESTLFKSNSTLNFSFENYRISKRDVADILYLVYQELGIKGFVNVILKQNIPNR
                     YFILLKNISEENFAKHENKWRNNSEINDSNIPYLKTSIYSLIA"
BASE COUNT          494 a          156 c          234 g          423 t
ORIGIN      
        1 ttacaaaaga aaatacacga tttattggtg taaaaaatat aaattctgtt gaaggatcga
       61 aaaatcgttt attgacagat tttaattgtg aaccaaattt tttattagat aatgattcac
      121 atgatattga ttcagtcgga aagaataata tgtggataaa aggtggaaag atttctttta
      181 aaatgttcca agaagctttg cttgattaca ctgtatcagt aagtttattt gaaccaaatt
      241 ttgaacaaaa gtcttacata aaaggtttat acattcaaag tcgtggaggc gatagaagtt
      301 ttcttacagg tgataagtta gaaaaagata gagacttctt tttaacattc tcaccatcta
      361 tgaactgttt aattggaggg agaggtacag ggaaaagtac actaatagat atgttacaat
      421 ttgtactttc tcaggattgc gacaagcaga gtaaattaga attcctatgt aaccatgcaa
      481 atgcttttgt actctatgtt ttagaggatg ctgaatatat cattgaggtt agtttgccag
      541 atgtcttgca ggaaaatagg gacaatatct tacaatatta tgggcaaaat cgtgagaata
      601 ggtatggata tccttataac tacaattctg actctatcaa agagtggaca cgctctcaat
      661 atacgaaagt atacaaagtt gagggaaaat ttttcaaatt agttgataaa actagaatat
      721 tagaaaaaat gtttgatagg agatattctg ttaatgagtt ggttagaaca gcggatggag
      781 aaaaaattac agaatttatt tccgacttaa tgctaaaaaa taaaaattta ccgagaccaa
      841 actatggttt aagaactcag actctagaat cattcgaagc taaactacag gaacttgata
      901 aatataggag agttcgtaag gattcaatac tcaaaataat tgatgatttc aatcaaactc
      961 aagttggtaa gttaagaatc tgttatgagc aaattgatag atgggaagta ccagattttg
     1021 aaagcactct tttcaaaagt aattcaactc tcaatttctc atttgaaaat tatagaatta
     1081 gtaagcggga tgttgcagat attctatacc ttgtttatca ggagttagga ataaaaggat
     1141 ttgttaacgt tattttgaaa caaaatattc ctaataggta ttttatatta ttaaaaaata
     1201 tttctgagga gaattttgca aaacatgaga ataagtggag aaataattcc gagataaatg
     1261 attctaacat accctattta aaaactagta tttattcttt aatcgca
//
LOCUS       DBNFCK010000203         1275 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.207, whole genome shotgun sequence.
ACCESSION   DBNFCK010000203 DBNFCK010000000
VERSION     DBNFCK010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.207"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..880
                     /locus_tag="AC1PIW_001930"
     CDS             <1..880
                     /locus_tag="AC1PIW_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HIP6606812.1"
                     /translation="QIILLTIQVFIVALILWMLYTILSYAKKHDISLKEKFWTGFGIG
                     YVTDLLDTLGIGTFATTTTMFKLTKLVEDDRKIPATMTTAHIIPVLVEALLFITIVEV
                     DMVTLIAMAAAAFTGAFVGAKVTQKWDTKKVQRILGILLIIAACFMVYRMITNPGADL
                     TNEVKGLTGWKLAVGIAFDFMVGMLMSMGLGNYAPELIFFSLMGISPAVALPVMMLNA
                     AMIMTAGAKQFIQSGRVNWPGVPGIVVGGVLGVLTAAFFLSNLDINNLKILVVFIAAY
                     TGFTLLRSSFVTRIKK"
     gene            892..>1275
                     /locus_tag="AC1PIW_001931"
     CDS             892..>1275
                     /locus_tag="AC1PIW_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HIP6606813.1"
                     /translation="MDIHLRNEIDLDGQIEVVDQQFQVEVKEKDGNLYLIYSNDEAEK
                     VVIKCDEEELVMTRFSTPKSIMRFISGKEAIVTIPTPLGIQHFVTDTKRYQLNRSDQS
                     VQLQYELKGLENQQLFASYDLEISWK"
BASE COUNT          360 a          216 c          291 g          408 t
ORIGIN      
        1 tcaaattatc ttattaacta ttcaggtctt tattgtagcc ctgattcttt ggatgcttta
       61 cacaattcta tcctatgcta aaaagcacga tatttctcta aaagagaaat tttggacagg
      121 atttggtatc ggttatgtta ctgatttgct agacaccttg gggattggaa cctttgcaac
      181 gacaaccact atgtttaaac tgacaaagtt ggtggaagac gatcgtaaaa ttcctgcgac
      241 catgacaacg gcgcacatca taccagtctt agtagaggcg ctattgttta ttacgatagt
      301 tgaggttgat atggtcactc tgattgctat ggcagcagcg gcatttacgg gtgcctttgt
      361 tggtgccaag gtaactcaaa aatgggatac aaagaaagtt caacgaattc ttgggatttt
      421 actaattatt gcagcctgtt tcatggtcta tcgcatgatt actaaccctg gcgcagatct
      481 taccaatgaa gtaaagggct taactggttg gaaactggct gttggtattg cctttgattt
      541 tatggtggga atgctgatga gtatgggact gggtaactat gctcctgagt taattttctt
      601 ctcattgatg ggaattagtc cagcggttgc tcttccagtt atgatgttga atgctgctat
      661 gattatgaca gctggtgcca agcagtttat ccaatcagga cgagtaaact ggccaggagt
      721 tccaggaatc gttgtcggtg gggtgctagg tgttttgaca gccgctttct tcctatcaaa
      781 tttggatatt aataatttga aaattttagt tgtcttcatt gcagcgtata caggttttac
      841 tctgcttcgt tcatcctttg ttactaggat taaaaaatag taggaggaac tatggatatt
      901 catttacgaa atgaaattga tctggacggg cagattgagg tagtggatca gcaatttcaa
      961 gtggaagtta aggaaaaaga tgggaatctc tatctcattt atagtaacga tgaagctgaa
     1021 aaagttgtca taaaatgtga cgaagaagag ttagtgatga cacgcttttc cactccgaag
     1081 tctattatgc gattcatttc tggtaaagaa gctattgtga ccattcctac accgttagga
     1141 atccagcatt ttgtgaccga tacaaaacgt taccaattaa accgttcgga tcaaagtgtc
     1201 cagttgcaat atgaattaaa gggattggag aaccagcagc tttttgcttc ctatgatttg
     1261 gaaatctcgt ggaaa
//
LOCUS       DBNFCK010000204         1269 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.208, whole genome shotgun sequence.
ACCESSION   DBNFCK010000204 DBNFCK010000000
VERSION     DBNFCK010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.208"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..1234
                     /locus_tag="AC1PIW_001932"
     CDS             <1..1234
                     /locus_tag="AC1PIW_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIP6606814.1"
                     /translation="NLIKEGYKISVYDESLEAYNQYRLDNKYLTKIIAEKNPDLIIPL
                     DADEFLTADSNPRKLLEQLDLEKIHYVNWQWFVMTKKDDINESFIPRRMQYCFEKPVW
                     HHSDGKPVTKCIISAKYYKKMNLKLSMGHHTVFGNPNVRIEHHNDLKFAHYRAISQEQ
                     LIYKTICYTIRDIATMENNIETAQRTNQMALIESGVDMWETAREASYSGYDCNVIHAP
                     IDLSFCKENIVIKYNELSRETVAERVMKTGREMAVRAYNVERKQKEKKFLKPIIFVLD
                     GLKGDEYIHPNPSNHLTILTEMYNVRGLLTDNHQIKFLKVNYRLIITPDFAKFLPHEF
                     IVVPDTLDIEQVKSQYVGTGVDLSKIISLKEYRKEIGFIGNLYALLGFVPNMLNRIYL
                     YIQRNGIANTIIKIKSRL"
BASE COUNT          470 a          167 c          230 g          402 t
ORIGIN      
        1 taatttgatt aaagagggat ataaaatatc cgtatatgat gagtctttag aggcatataa
       61 tcagtatcga cttgataata aatatctaac gaaaataatt gctgaaaaaa atccagattt
      121 gataatacct ttggatgcgg atgaattttt aacagccgat tcaaatccac ggaaactttt
      181 ggaacaactg gacttagaaa agatacatta tgtgaattgg caatggtttg ttatgactaa
      241 aaaagatgat attaatgaat cgtttatacc acgtagaatg caatattgtt ttgaaaaacc
      301 tgtttggcat cattctgatg gtaaaccagt tactaaatgt ataatttccg ctaagtatta
      361 caaaaaaatg aatttaaagc tatcgatggg acatcacact gtttttggta acccaaatgt
      421 aaggatagaa catcataatg atttgaaatt tgcacattat cgagctatta gccaagagca
      481 attaatttat aaaacaattt gttacactat tcgcgatatt gctactatgg agaacaatat
      541 cgaaacagct caaagaacaa atcagatggc gctcattgaa tctggcgtgg atatgtggga
      601 aacggcgaga gaagcctctt attcaggtta tgattgtaat gttatacatg caccaattga
      661 tttaagtttt tgtaaagaaa atattgtaat aaaatataac gaactatcca gagaaacagt
      721 agcagaacgc gtgatgaaaa cgggaagaga aatggctgtt cgtgcatata atgtggagcg
      781 aaaacaaaaa gaaaagaaat ttctaaaacc tattatattt gtattagatg ggttaaaagg
      841 agatgagtat attcatccca atccatcaaa tcatttgacg atcttaactg aaatgtataa
      901 cgtcagaggc ttacttaccg ataatcacca aattaaattt ctcaaagtta attatagatt
      961 aattataact ccagattttg ctaagttttt accgcatgaa tttattgttg taccagatac
     1021 cttggatata gagcaagtta aaagccagta tgttggtaca ggtgtagact tgtcaaagat
     1081 tatttcttta aaagagtatc gaaaagagat aggctttatt ggtaatttgt atgcgctttt
     1141 aggatttgtt ccgaatatgc tcaatagaat ttatctatat attcagagaa acggtattgc
     1201 aaacactatt ataaaaatca agtcgagatt gtgagagttg tttactttta tttgtaattt
     1261 taaaagtaa
//
LOCUS       DBNFCK010000205         1215 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.209, whole genome shotgun sequence.
ACCESSION   DBNFCK010000205 DBNFCK010000000
VERSION     DBNFCK010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.209"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..966
                     /locus_tag="AC1PIW_001933"
     CDS             <1..966
                     /locus_tag="AC1PIW_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HIP6606815.1"
                     /translation="PYVCNACPKKRSNCGYQKQFYYAKRAQLDYEVKLSDSRTGVALN
                     KEEFYRMDEIVSAAIQKGQHLNHIIASNEMSASRASIYRYLEKGYLSTKPIDFPRVVK
                     FRKRRIRNLQPIPKIAKEGRSYEDFQRFLTEKGISYWLEMDTVTGRIGGKVLLTFNLS
                     FCNFIFAQLLDNKTANEVAKHLYAIKNDLHQKEMDFCEIFPVILTDNGGEFARVDDIE
                     MDVRGESKLFFCDPNRSDQKGRIEKNHTLIRDILPKRSSFDNLTQEDINLVCSHINSV
                     KRASFNGKSAYELFTFTYGEELATLLGISKIDPENVIQSPRLLDK"
BASE COUNT          389 a          267 c          230 g          329 t
ORIGIN      
        1 ccttacgttt gtaatgcctg tccgaaaaag agaagcaact gcggatacca gaaacagttc
       61 tactacgcaa aaagagctca gcttgattat gaagttaagc tctcagattc gagaacaggt
      121 gttgcactaa acaaggaaga attctatcgc atggatgaga ttgtttctgc tgccatccaa
      181 aagggacaac acctcaacca catcatcgcc tcaaacgaaa tgtcggcatc cagagcttct
      241 atctaccgat accttgaaaa aggctatctg tccacaaagc ccattgattt cccccgtgtc
      301 gtgaaattca gaaagcggag aatcagaaac ctacaaccca ttcctaaaat cgccaaagaa
      361 ggacggtctt acgaggactt ccaacgcttt ctcacagaga aaggaatcag ctattggctg
      421 gaaatggaca ccgttactgg acggatcggc ggaaaggtac ttctcacctt taacctctcc
      481 ttctgtaact ttatcttcgc tcaattactt gataataaaa cagctaatga ggtcgctaaa
      541 catctctacg ctatcaagaa tgacctgcat cagaaagaga tggacttttg cgaaatattt
      601 cctgtcattc tgaccgataa tggcggtgaa ttcgctagag tggacgatat cgaaatggat
      661 gttcgcggag aatctaagct attcttctgt gaccccaatc gttctgacca gaaggggaga
      721 attgagaaga atcacacact tatcagagat attcttccta aaagaagttc ttttgacaac
      781 ttgacacagg aggacatcaa cctggtttgt tcgcatatca acagcgtcaa acgagcttct
      841 ttcaacggaa aatcagccta tgaactcttt acatttacct acggtgagga attggcaaca
      901 cttttgggaa tctctaaaat tgaccctgag aacgtcattc aatcacctcg attattggat
      961 aagtaatcgc tagtttttat caaaaaataa tttcaaaata gaaaggaact tgttccatcc
     1021 aaaattccag atagctagaa cttactttga gacgtctcag agccagtaac tttagtgtac
     1081 tcttttttca acattttcaa ccctaaaact cactattatc aacattttta gtcaaaaaca
     1141 gaacttagtc tgagactaaa ttctgttgat gttatgcagt tttctcagag taacttctgg
     1201 aaggacggga actag
//
LOCUS       DBNFCK010000206         1214 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.210, whole genome shotgun sequence.
ACCESSION   DBNFCK010000206 DBNFCK010000000
VERSION     DBNFCK010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.210"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            159..914
                     /locus_tag="AC1PIW_001934"
     CDS             159..914
                     /locus_tag="AC1PIW_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606816.1"
                     /translation="MALGNVEKDTEGWIELINQYLQYCIEIGLSPYTQATYKVALAKV
                     LGVSSTNFIATQPRTRANRMNNRVLHKDYRLSNKNNDYWHKVVTSTGLRKSELIHVTG
                     DALQRGRDGRWYLNLAGHKHHTKGRRDRWSPIMATSQEEEEWLVAIFQRAGKKKVFHV
                     PKDLILDDFDGKKVPTALKSHKYPAEYAERVYRSVAREISKIRNRKEIIHLRKELVGI
                     SLDRKACKIVTKALGHNRPEEFPHSYAYILLKR"
     gene            1003..1191
                     /locus_tag="AC1PIW_001935"
     CDS             1003..1191
                     /locus_tag="AC1PIW_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIP6606817.1"
                     /translation="MLPQNNSPLLLNRQQVAELLGIDPKSFGKYIRSHPDFQCFMVGK
                     QERYLKSKLVKFIESHCD"
BASE COUNT          416 a          228 c          234 g          336 t
ORIGIN      
        1 ttttgcgcag ttcgcaaaac atcggttcgt tagaaccgac tgcaaggaaa aaagaaagca
       61 ttgagttaat ccatactcat cgtacactcc ataactattc aggttcatag cattgttttg
      121 caaaatgggt ttcaattaac atttcgccag aacagttaat ggcattaggt aatgttgaaa
      181 aagatacgga aggttggata gaacttatca atcaatactt acaatattgt atagagattg
      241 gtttaagtcc atatacacaa gctacctaca aagtagcatt ggccaaagtt ctcggagttt
      301 cctcaactaa tttcatcgct acacaaccac gtactcgtgc gaatcgtatg aacaatcgtg
      361 tattacataa agactatcgt ttatccaaca aaaataacga ctactggcat aaagtagtta
      421 cctcgacagg cttacgcaaa agtgaattaa tccacgtcac tggcgatgct ctgcaacgcg
      481 gccgtgatgg ccgttggtat ttaaatttag ccggccataa gcatcacaca aaaggacgca
      541 gagatcgctg gtctccaatc atggccacta gtcaagagga ggaagagtgg ttagtcgcta
      601 ttttccaacg agcaggaaaa aagaaagtct ttcatgttcc caaagattta atcctcgacg
      661 attttgatgg aaagaaagtt ccaacagcat taaagtcaca caaatatcct gcagagtatg
      721 cagagcgtgt ttatcgcagt gtagcacgcg aaatttccaa gattagaaat cgtaaagaaa
      781 ttatccatct gcgtaaagag ttagttggca tctcgctcga cagaaaagct tgtaaaattg
      841 taacaaaggc tctgggacac aatagaccag aggaatttcc acattcctat gcttatattt
      901 tattgaaacg ttaggaaaca ttgtacattt ttcaaaaatc aagcctcgat ttgttgcaca
      961 tgtggcataa tacttatatc tagaaaatga aaggaatttt atatgttacc tcaaaataat
     1021 tctcctcttc tgttaaatag acaacaagta gcggaactac ttggaataga tcctaaatca
     1081 tttggtaagt acattagaag tcacccagac tttcaatgct ttatggtggg aaaacaagag
     1141 cgctatttga agtcaaaact ggttaaattt attgaaagcc actgcgattg acggataatg
     1201 attgataaaa ttag
//
LOCUS       DBNFCK010000207         1202 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.211, whole genome shotgun sequence.
ACCESSION   DBNFCK010000207 DBNFCK010000000
VERSION     DBNFCK010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.211"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..759
                     /locus_tag="AC1PIW_001936"
     CDS             <1..759
                     /locus_tag="AC1PIW_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002943315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIP6606818.1"
                     /translation="IDKIKVNNLSFIYPGSVKKTLEDINVEFNKGELVAIVGKNGSGK
                     STLVKLLSGLYQPSEGQIYYNNESSDVLDLSYYQNNVSVLFQDFVKFELSVRENIGLS
                     DVNSISDSKIKKHIDNLKLNFLTAENNFNLNQRLGTWFNDSRQISGGQWQKVALARTF
                     FKNASIYILDEPSSALDPVSEKEIFDEFVTRSRDKIALFVSHNLMAASRADRIIVMQD
                     GRIIDEGKHDDLISKSKYYRELYYSEKYEEESDG"
     gene            752..>1202
                     /locus_tag="AC1PIW_001937"
     CDS             752..>1202
                     /locus_tag="AC1PIW_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="HIP6606819.1"
                     /translation="MDENKVIIDLSEKVFAKFDEQLKRYAEQPNYDLLTLSSGLPGLI
                     LLSSELTSLTSERKYSARTGKYVNFMVKQMRNYGVLSDSLFSGVSGIGISILHLVEEH
                     PEYHNLLISFNEYIKYYTLSKIENIDIKKISPTDYDIIEGVSGVLVYL"
BASE COUNT          430 a          147 c          227 g          398 t
ORIGIN      
        1 attgacaaaa taaaagtcaa taatctctct tttatctatc caggaagtgt caaaaaaact
       61 ttagaggata ttaatgttga gtttaataag ggtgaacttg tagcaattgt ggggaagaat
      121 ggttctggta agagtacctt agtaaaatta ctatcgggat tataccaacc ttcagagggt
      181 caaatatatt ataataacga atctagtgat gtcctagact tgagttatta tcaaaataat
      241 gtttctgttc tttttcagga ctttgtgaag tttgaattat ctgtgagaga aaatattggc
      301 ctaagtgatg taaacagtat atctgactcc aaaataaaga aacatattga taacttaaaa
      361 ttaaatttct taaccgcaga aaacaatttc aaccttaatc aacgtttagg gacctggttt
      421 aacgatagta gacaaatttc aggaggacaa tggcaaaaag tagcattggc taggactttc
      481 ttcaagaatg caagcatata cattttggat gaaccaagtt cagcgcttga tcctgtttca
      541 gagaaagaaa tatttgatga atttgtaact cgttctagag ataagattgc tttgtttgtg
      601 tcacataatc taatggctgc gagtagagca gatcgaatta tagttatgca agatggacga
      661 attattgatg aaggaaaaca tgacgacctt ataagtaaat ctaaatatta tagggaactt
      721 tactattcag aaaagtacga ggaggagtct gatggatgaa aataaagtta ttattgattt
      781 atctgagaaa gtatttgcta aatttgatga gcagttaaaa agatatgctg aacaaccgaa
      841 ttatgattta ttgaccttgt cttctggttt acctggattg atattattat cttcagaatt
      901 gacaagttta actagtgaaa gaaaatactc cgctagaacg ggtaaatatg ttaactttat
      961 ggttaaacaa atgagaaatt atggtgtttt atcggattct ttattttcag gagtttctgg
     1021 aattggaata agtatacttc atttagttga agaacaccca gaatatcata acttattgat
     1081 tagttttaat gagtacatta agtactatac gttaagtaag attgaaaata tagatattaa
     1141 gaaaatctct cctacagatt atgacatcat agaaggagta tctggagtat tagtttatct
     1201 tt
//
LOCUS       DBNFCK010000208         1191 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.212, whole genome shotgun sequence.
ACCESSION   DBNFCK010000208 DBNFCK010000000
VERSION     DBNFCK010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.212"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            32..1054
                     /gene="ilvC"
                     /locus_tag="AC1PIW_001938"
     CDS             32..1054
                     /gene="ilvC"
                     /locus_tag="AC1PIW_001938"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HIP6606820.1"
                     /translation="MTVTMQYEKDVTVAALDGKRIAVIGYGSQGHAHAQNLRDTGHDV
                     IIGVRAGKSFDKAKEDGFETFEVAEAAKQADVIMILAPDEIQADLYNEEIAPNLEAGN
                     ALGFAHGFNVHFEFIKVPADVDVFMCAPKGPGHLVRRTFEEGFGVPALYAVYQDATGN
                     AKHIAMDWAKGVGSARVGLLETTFKEETEEDLFGEQAVLCGGLTALMQAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLVYEGGFKKMRQSISNTAEFGDYVSGPRVITDQVKENMK
                     AVLADIQSGKFANDFVNDYKAGRPRMEAYRKEAENLEIEKVGAELRKAMPFVGRNDDD
                     AFKIYN"
BASE COUNT          371 a          220 c          281 g          319 t
ORIGIN      
        1 ctacaaataa aaatagaaaa gagaaaaaat tatgacagta acaatgcaat atgaaaaaga
       61 tgtaacagta gcagcacttg acggtaaacg tatcgccgtt atcggttatg gttcacaagg
      121 tcatgcccat gctcaaaact tgcgtgatac aggacacgat gtcatcatcg gtgtgcgtgc
      181 aggtaagtca tttgacaagg caaaagaaga tggttttgaa acttttgaag tagcagaagc
      241 agcaaaacaa gcagatgtta tcatgatctt ggctccagac gaaatccaag cagaccttta
      301 caatgaagaa atcgctccaa acttggaagc gggcaatgcc cttggttttg cccatggttt
      361 caacgttcat tttgaattta tcaaggtacc agcagatgtg gatgtcttca tgtgtgcacc
      421 aaaaggtcca ggtcacttgg tccgccgtac ctttgaagaa ggttttggag taccagccct
      481 ctatgctgtt taccaagatg caactggcaa tgcaaaacat attgcgatgg actgggcaaa
      541 aggtgttggt tcagcccgtg ttggtctttt ggaaacaact ttcaaagaag agacagaaga
      601 agatttgttt ggtgagcaag ctgttctctg cggtggtttg acagccctta tgcaggcagg
      661 ttttgaagtc ttgacagaag caggttatgc accagaattg gcttatttcg aagttctcca
      721 cgagatgaaa ctcatcgttg accttgtcta cgaaggtggc tttaagaaaa tgcgccaatc
      781 tatctcaaac actgctgaat ttggtgacta tgtatcaggt ccacgtgtga ttacagacca
      841 agtcaaagaa aacatgaagg cagtccttgc agatattcaa tctggtaaat ttgcaaatga
      901 ctttgtaaat gactataaag caggccgtcc acgtatggaa gcttatcgta aagaagcaga
      961 gaatcttgaa atcgaaaaag taggtgcaga gctccgcaaa gcaatgccat ttgtcggacg
     1021 caatgatgac gacgcattca aaatctataa ttaaacacta tcatggtgga taggaggata
     1081 gtcagcttta ctttgagtga tggcaataca tttaaggttt gagttggcgg atgaggggat
     1141 gtcattccct tttttcccta tctctgaaaa tgaataaaag agctggtttt t
//
LOCUS       DBNFCK010000209         1183 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.213, whole genome shotgun sequence.
ACCESSION   DBNFCK010000209 DBNFCK010000000
VERSION     DBNFCK010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.213"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            69..>1183
                     /locus_tag="AC1PIW_001939"
     CDS             69..>1183
                     /locus_tag="AC1PIW_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606821.1"
                     /translation="MLNKEWYFKNFNMVSELDISGEFIYTGISIVNNMDRIAPEMPTE
                     LFLALYNISVGVERLQKIILVLWEFDEVDSIEDFSESLKIHNQVELHSRIEKAINKKI
                     FSKQECQFLELLKEFYSNARYDRFSAVGNRWEYELISKFFSNNQISYEESVSDSQVIL
                     INDSIRKLIGRVLSRIVKNYYLLVEEGSNKSGTYSYELRNDSKAQKIFLHMENRRGLS
                     SIKIDERIALSEILIYLRNTSDKSSYLKFIEGIYPLDFDKMEISRYLSIILLEDRIPQ
                     DLIDEIEYIYAEKDIKVGERIENLDLLTKNNMQVTFDYPYLKEVYIILEEVKKQESIN
                     DDTISRLEESNCYIDDSDTQEIIESIIAVARQYRNNK"
BASE COUNT          460 a          123 c          218 g          382 t
ORIGIN      
        1 ctaactcaat atgataggga gaagatatta attcagttga ataaataaaa attttaggga
       61 gatatagggt gttaaataaa gagtggtatt tcaaaaattt taatatggtt tcagagttgg
      121 atatctcagg agaatttata tatactggta tttccattgt aaataatatg gatagaattg
      181 ctccagagat gcctacggaa ttatttttgg ctttatacaa catttctgta ggagtcgaaa
      241 gactacagaa aataatatta gtgttgtggg agttcgatga ggttgattcc atagaagact
      301 tttctgaaag tctaaagatt cataatcaag ttgaattaca tagcagaatt gaaaaagcga
      361 tcaataaaaa gatttttagt aaacaagaat gccaattttt agaactttta aaagagtttt
      421 attctaatgc gaggtatgat agattttcag cagtggggaa tagatgggaa tatgaactta
      481 ttagtaaatt tttctctaat aatcaaatca gttatgaaga atcagtttct gattctcaag
      541 taattttaat caacgatagt attaggaaat tgatagggcg agtgctctct agaatagtaa
      601 aaaactacta cctacttgtg gaggaaggaa gtaataaatc aggaacttat tcatatgaac
      661 tcagaaatga ttcaaaagct cagaagatat ttttacatat ggagaatcgc cgaggattaa
      721 gttccataaa aatagatgaa cggatagctt taagtgaaat tcttatttat ttgagaaata
      781 catctgataa atcgtcatat ttaaaattta ttgagggaat atatcctctt gattttgata
      841 aaatggaaat tagtagatat ttatcaataa ttttactaga ggatagaatc cctcaagact
      901 taattgatga aattgaatat atttacgcag aaaaagatat taaggtagga gagcgaatag
      961 aaaacttaga tttattaaca aaaaataata tgcaggttac attcgattat ccttatttga
     1021 aggaagtata tataatttta gaagaagtca aaaaacagga aagtattaac gatgatacaa
     1081 taagtaggtt agaggagtct aactgttata ttgacgatag tgatactcaa gaaattatag
     1141 aatctatcat tgctgttgct cgccaatatc gcaataataa aat
//
LOCUS       DBNFCK010000210         1164 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.214, whole genome shotgun sequence.
ACCESSION   DBNFCK010000210 DBNFCK010000000
VERSION     DBNFCK010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.214"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(751..>1164)
                     /locus_tag="AC1PIW_001940"
     CDS             complement(751..>1164)
                     /locus_tag="AC1PIW_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIP6606822.1"
                     /translation="FGVGKTTLANILHKRIENSYLYNPELLGDFLQHQLPQTVCPEDF
                     QDYSVWRQSTYKIVFDLATKTDKIIIIPMTIYKKEYYQEIIQQLIQDKIPLEHYILLA
                     DKTTILERLDNRVNEDNIWAKRHLDDCLYSMKIKI"
BASE COUNT          369 a          211 c          160 g          424 t
ORIGIN      
        1 gctcagcttt tttcttgtcc tgctctccag aaaacagcat gcctcttgat tctaacaaag
       61 caacttgttc ctcaaaactt ttatgggaaa aactactcat ttgaacactc cttaaacaaa
      121 aaaaccaccc atcagaacat gtctgtgcaa ttgaagcaag ggcggccatt gatatttcaa
      181 gttcattata tagtatattt gaatatacgt caagtttttt atcaagaaaa tctgagaaat
      241 tagctgattt tattattttt gctctacgac taacatctaa aaatgtacaa ttgctccaat
      301 ctcctcccct ctattttcta ctacattttg tacaattgga tttctaacac taatacaata
      361 atatgcttta atcctgtacc ttattgaatc ctaaaataat gtacaaaata aaaataaagt
      421 acattttttg aagaatgtac tttatgcttc tatctcagta ttatcaaggg ttcaggctat
      481 attaggtcaa ttgttcataa ttttttagtt ttcgagataa ctagaaaatt tttttcacgt
      541 ccaaaatcct aaaaatctag attatttctc ggtaaactca catagcatta gaatcttctt
      601 cgcaacctct tctggcttca aactgtctgt tttaaacctt tgtcctggaa tctgactttc
      661 aaaagatttt aatactcttc gaaaatcaaa atcagacgtt gttgacttgt tttgatgaac
      721 ttcagttcta tctgcatcta cgtcgcctag tcagattttg attttcattg agtataaaca
      781 atcatcaaga tgtcttttgg cccagatgtt gtcttcattg actcgattat ctaaacgttc
      841 caaaatggtt gttttgtcag ctaacaaaat ataatgttcc aaaggaatct tatcctgtat
      901 caattgctga ataatctcct gataatattc tttcttatag attgtcatag gaataataat
      961 aatcttatcg gttttagtcg ctaaatcaaa tactatttta taagtactct gtcgccaaac
     1021 ggaataatct tgaaaatctt caggacatac agtctgaggt aattgatgtt gtaagaaatc
     1081 acctagtaat tcgggattat agagatatga gttttctatt cgtttgtgta atatatttgc
     1141 taaagtagtc tttccgacac cgaa
//
LOCUS       DBNFCK010000211         1125 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.215, whole genome shotgun sequence.
ACCESSION   DBNFCK010000211 DBNFCK010000000
VERSION     DBNFCK010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.215"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(267..734)
                     /locus_tag="AC1PIW_001941"
     CDS             complement(267..734)
                     /locus_tag="AC1PIW_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HIP6606823.1"
                     /translation="MTIRKARQSDIPILNELLQDILQVHHQARPDIFKSAGQKFSEAE
                     LVALLENPDKPIFVFELEGQVVGHLFCELSTATGDVLEPIKTLFIDDLCVANSARGQR
                     IGEQLYEFALSYAKEQGCHNLTLDVWADNAGAVRFYERQGMKPQKFRMEQIIE"
BASE COUNT          346 a          236 c          205 g          338 t
ORIGIN      
        1 cacaaaaaaa aagactacta gggacgagct agtagcctaa cataaaaaat ttttcgcttt
       61 gattatatca tagggaggga gtttttgcat tcttttgagt tttcgtataa gaactcgacc
      121 aagataaaaa gagttcgtct tcccaccccc gcacagttga ttaggccaga tttggagtgc
      181 aaaacacgaa caaatctgcc aatcaaccac tgcgctgaga tgttgacacg aactctgagt
      241 agtggtcctg ggctttttgc ccagcctcac tctattattt gctccatacg gaacttttgt
      301 ggtttcattc cttggcgttc ataaaaacgg acagctcctg cattgtcagc ccatacatcc
      361 aaggtaagat tgtggcaacc ctgctcctta gcataggaaa gagcaaattc ataaagctgc
      421 tcgccaattc tttgaccacg cgcagaattg gctacacaca aatcatcaat aaacaaggtt
      481 ttaattggtt ctaaaacatc gcctgttgcg gtagataatt cacaaaaaag atgcccaacc
      541 acttgtccct ctaactcaaa gacaaaaata ggcttatctg gattttccaa aagtgctact
      601 aattcagcct cagaaaattt ttgacctgca cttttaaaaa tatctggtcg tgcttggtga
      661 tgcacctgta aaatatcttg aagcaattca tttaatatcg gaatgtcaga ttgtctagct
      721 ttacggattg tcataatact ctccttttga gtttttatta ttatagcaca tgttcatttt
      781 ttaacaaaat aggtctacag accgatgaag aatctgaaaa ttcgagtata ctataggtgt
      841 atcctaaaat ttttcataaa tctcctaaaa aatcagctgg ccaaggctgg ttttttgttg
      901 tcttaaaacg agaatgtgta gtgtagaaaa ctactaaagt ttctctctct ttgacgcaaa
      961 atgcttttgc ttcatttcca acctcccaga gtcactcccc tgactctggg agccccaaaa
     1021 cacagaaaca tactaagatt agtagtgagg aaatctccga cgggagaaag tactcactac
     1081 tttttcttta tgataaagta gaggtgtctt gttaagtcgt agggc
//
LOCUS       DBNFCK010000212         1065 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.216, whole genome shotgun sequence.
ACCESSION   DBNFCK010000212 DBNFCK010000000
VERSION     DBNFCK010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.216"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..326
                     /locus_tag="AC1PIW_001942"
     CDS             <1..326
                     /locus_tag="AC1PIW_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HIP6606824.1"
                     /translation="KLSRPSTFISKEEFVKIRKQPRIVHFLGEERPWRIGNKHRFRED
                     YVSALNQTPWRGTQFESGWQFYFFCFNLFNMVMKPFPMLRYKIITVLIPVFMKYRKIR
                     LQKGV"
     gene            329..>1065
                     /locus_tag="AC1PIW_001943"
     CDS             329..>1065
                     /locus_tag="AC1PIW_001943"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIP6606825.1"
                     /translation="MKFSVIIPAYNVADYLEECVYSVLNQTYEEFEILLVDDGSTDGK
                     TSNICDKLAAKDDRVKVFHQTNGGLSAARNTGIKNASGDYILFLDGDDFWTDICFLDE
                     VNREIISHDEVIDAVIYPFSYFYGKDDVAVRNFSKKLPFHQVITDTRLLVETGALITP
                     AWNKCVRRDKFTSALNFLSGYLYEDGIWCANLLKELNVFIVVDNTQCMYRQNRQGSIT
                     NNVTLRHVEHAFRGIEENLKNFKQLSN"
BASE COUNT          351 a          143 c          217 g          354 t
ORIGIN      
        1 aaaaattaag taggccttca acgtttattt caaaagaaga atttgtaaaa attcgtaagc
       61 aaccacgtat tgttcatttt ttaggtgaag aacgtccatg gagaatcgga aataaacatc
      121 gatttagaga ggactatgtt tctgcattaa atcagacgcc atggagaggt actcaatttg
      181 agagtggttg gcagttttac tttttctgtt ttaatctttt taacatggtt atgaagcctt
      241 ttccaatgtt gcgttataaa attattacag tcttaattcc tgtattcatg aaatatagga
      301 aaattagatt acaaaaagga gtctaggtgt gaagttttcc gttattatcc cagcgtacaa
      361 tgttgctgat tatctggaag aatgtgtgta tagtgtactg aatcagactt atgaagagtt
      421 tgaaatattg ttggtagatg acggttctac agatggtaaa acctccaata tatgcgataa
      481 attagcagct aaagatgata gggtgaaggt atttcatcaa actaatgggg gattatctgc
      541 agccagaaat acagggataa agaatgctag tggtgactat attttatttc tggatggaga
      601 tgacttttgg accgatatat gctttttaga tgaagttaat agagaaataa tttcacatga
      661 tgaagttatt gatgcagtta tctatccatt ttcctacttt tacggtaagg atgatgtagc
      721 agttcgaaat ttttctaaaa aattaccgtt tcatcaggtt attacagata cgcgcttact
      781 cgttgaaaca ggagccctaa tcacccctgc ttggaataag tgtgtcagaa gagataaatt
      841 tacctctgct cttaatttct tgagtggata tttatatgaa gatggtattt ggtgtgcaaa
      901 tctcttgaaa gagttgaatg tatttattgt tgtagataat acacaatgca tgtatcgtca
      961 gaaccgacaa ggaagtatta caaataatgt cacgcttcga catgtagaac atgcctttag
     1021 aggaattgaa gaaaatttaa agaattttaa acaactatcg aatga
//
LOCUS       DBNFCK010000213         1049 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.217, whole genome shotgun sequence.
ACCESSION   DBNFCK010000213 DBNFCK010000000
VERSION     DBNFCK010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.217"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..890
                     /locus_tag="AC1PIW_001944"
     CDS             <1..890
                     /locus_tag="AC1PIW_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIP6606826.1"
                     /translation="EFGQRVRQLRESASMTREQFCDDELELSVRQLTRIEAGASKPTF
                     SKIQYIATRLGMGLYELMPDYVSLPERYSKLKFDVLRTPTYGNEDLAEKRDAMMTEIY
                     DDYYDELPEEEKIAIDAIQSRIDTLESGTAGFGKEILEDYFEQIFRKRKYELNDLLIV
                     RLHLEYVRLSSCDSEIFRQFLKIIEHLHEQINIINSNDLFVLRDTLLSCVNILGSKKY
                     YEPIPKIFDSVDKIIQSTQDFQKKPIVSVLKWKYALFVDKDRDEAEKHYLDAVLFAKL
                     IENRELEQKIEEDWRVDNQ"
BASE COUNT          362 a          139 c          229 g          319 t
ORIGIN      
        1 aggaatttgg acagcgtgta cgtcaattac gagaatctgc tagtatgaca cgtgaacagt
       61 tttgtgacga tgaactggaa ctctctgtgc gccaattaac tcgtattgaa gcaggtgctt
      121 ccaagccgac tttttcaaag attcagtata ttgcaactcg tttaggtatg ggactttacg
      181 agcttatgcc agattatgta tctttacccg aaagatattc caagctgaag tttgatgtgc
      241 ttcgcacccc aacttatggt aatgaagatt tggcggaaaa gcgagatgcc atgatgacag
      301 aaatctatga cgattattat gatgaattgc ctgaggagga gaagatagca atagatgcga
      361 ttcaatcacg aattgatact ttagagtcag gtacagcagg ctttggaaaa gagatactgg
      421 aagactactt tgaacaaatt tttcgcaaac gaaagtatga attgaatgat ttgttgattg
      481 ttaggctcca tcttgaatat gttaggttat ctagctgtga ttcagaaata tttagacagt
      541 ttttgaaaat tatagagcat ttacatgagc aaatcaatat catcaactca aatgatttat
      601 ttgttttacg agacacgcta ttatcttgtg taaatatatt aggaagtaaa aaatattacg
      661 aaccaatacc aaagatattt gacagtgtag ataagattat acagtcgaca caagattttc
      721 agaaaaagcc cattgttagt gtattaaaat ggaaatatgc actttttgtg gataaggatc
      781 gggatgaggc agaaaagcat tatctagatg cggtgctatt tgcaaaattg atagaaaata
      841 gagagttaga acagaagatt gaagaagatt ggagagttga caatcaataa agtgacattt
      901 ttgtcctgtt gaagaattgt gaaatgcatt atactaaact cataaagatt aaaggagatt
      961 agtaacatgt ttaactattt gaaatttttt ggtcgtcttg gcggtaactg gggaacatgg
     1021 gttgaagaat aatattatta aaaagggag
//
LOCUS       DBNFCK010000214         1049 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.218, whole genome shotgun sequence.
ACCESSION   DBNFCK010000214 DBNFCK010000000
VERSION     DBNFCK010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.218"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..>1049
                     /locus_tag="AC1PIW_001945"
     CDS             <1..>1049
                     /locus_tag="AC1PIW_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606827.1"
                     /translation="INLPEYKSLSDAFQNGQRNDYENSMLYSKICFNYLDRGRIEDFS
                     HTKVEADDSKEREKQKLKAQKIQGLILSMNEEYRMAFMLYQLFYTDRTQWDENLAFYD
                     NNIQSIMKHGKQYHGYLFDQVKNIILSETDIDHRITESFLDKIWETKNETIMDFSWFD
                     QFGDQHQMSNFKILYVQWILSGATTSYLKSRINFERGFPIKVRNSLVGKIKGKENRKM
                     SVKDLKWRIRRKEERIGHLCRDYLLLTDQLTTIFTKVPSDKHNLQISVEYLLHSGKVN
                     LSDVIDNISVTSLLRLEWLLRWNYYHYHRESNYTSRTFFDEMTSNDRYYWSAGRGILE
                     FFIAKIVDNFYRDLY"
BASE COUNT          392 a          125 c          193 g          339 t
ORIGIN      
        1 atataaattt accagaatat aaaagtctat ccgatgcgtt tcaaaatggc cagcgaaatg
       61 attatgaaaa cagtatgctt tattctaaaa tttgtttcaa ttatttagat agaggaagaa
      121 ttgaagattt tagtcataca aaagttgaag cagatgatag taaagagcgg gaaaaacaaa
      181 aattaaaagc tcaaaaaata cagggattga ttctgagcat gaatgaagaa taccgaatgg
      241 catttatgct atatcaatta ttttatacgg atcgtactca atgggatgag aatctggcat
      301 tttacgataa taatattcaa agtataatga agcatggtaa gcaataccat ggttatttat
      361 tcgatcaagt gaaaaatatt attctaagtg agactgatat tgatcatcgt attacagaat
      421 cttttttaga caagatatgg gaaacaaaaa atgaaacaat tatggatttt tcatggtttg
      481 atcaatttgg ggatcaacat cagatgtcga attttaaaat tctttacgta caatggatat
      541 tatcaggagc gacaacttcc tatttgaaga gtagaattaa ttttgaaaga gggtttccta
      601 ttaaagtacg caattcactt gttggaaaaa taaaaggaaa agaaaatagg aagatgtcag
      661 ttaaagattt gaaatggagg attagacgta aagaagaaag gattggtcat ctttgccgag
      721 actatctatt attaacagat cagttaacga ctatttttac aaaagtacca tctgataaac
      781 ataatttaca aatatcagtt gaatatcttt tgcacagtgg taaagtgaat ttatcggatg
      841 ttatagataa tatatcagta acaagtttac ttcgcttgga atggcttttg agatggaact
      901 attatcatta tcatagggag tcaaattata ctagcagaac attctttgac gaaatgacct
      961 ctaatgaccg atattattgg tctgctggac gtggtatttt agaatttttc attgccaaaa
     1021 ttgttgacaa tttttatcgt gatttatac
//
LOCUS       DBNFCK010000215         1026 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.219, whole genome shotgun sequence.
ACCESSION   DBNFCK010000215 DBNFCK010000000
VERSION     DBNFCK010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.219"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            72..1001
                     /locus_tag="AC1PIW_001946"
     CDS             72..1001
                     /locus_tag="AC1PIW_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HIP6606828.1"
                     /translation="MEKIIITATAESIEQVKELLVAGVDRIYVGEADHALRIPTNFTY
                     DELREIAELVHSAGKELTVAANALMHQDMMDNIKPFMELMREIKVDYLVVGDTGIFYI
                     NKRDGYNFKLIYDTSVFVTSSRQINFWKDHGAVEAVLAREIPSEELFDIAKNLEMPAE
                     ILVYGASVIHHSKRTLLQNYYNFTKADETDLSRGRGLFLAEPSDPDSHYSIFEDKHGT
                     HIFINNDIDMMTKLSELDEHGYHNWKLDGIYCPGQNFVEIVKLFVQARDLIEKGDFTY
                     DQAFLLDEQVRKLHPAERGLDTGFYEFDRNKVK"
BASE COUNT          318 a          185 c          214 g          309 t
ORIGIN      
        1 tagaaaagtc tgaatattaa aacacggcta gtcacttatc agatatttct ctttttgata
       61 taattatcct tatggaaaaa attatcatta cagcaacagc tgaaagtatt gagcaggtca
      121 aagaactgct tgtagcaggc gttgaccgca tttatgtagg tgaagctgac catgctcttc
      181 gcatcccaac aaattttacc tatgatgaac tcagggaaat agcagagcta gttcatagcg
      241 ctgggaagga attgacggtc gctgccaatg ccctcatgca tcaagatatg atggacaata
      301 tcaaaccgtt tatggagcta atgagggaaa tcaaggttga ttatctggtt gtcggagata
      361 ctggaatttt ctatatcaat aaacgtgatg gctacaattt caagctaatc tatgatacat
      421 ctgtctttgt gacttccagt cgtcagatta acttttggaa agatcatgga gcagtagagg
      481 ctgttttggc aagagagatt ccatcagaag aactctttga tattgctaag aacttggaaa
      541 tgccagcgga aatcttggtc tacggcgcct ctgttattca ccattctaaa cgaaccttgt
      601 tacagaatta ctacaatttt acaaaagctg atgagacaga tttgtcacgt ggacgcggac
      661 ttttcttagc tgagccgagt gacccagata gccactattc catttttgaa gataagcatg
      721 gcacccatat ctttatcaac aatgacattg atatgatgac caagctgtca gaattagatg
      781 aacatggtta tcataattgg aaacttgatg gtatctattg ccctggtcag aactttgtgg
      841 aaattgttaa gctttttgtt caagcaagag atttgattga aaaaggcgac ttcacttatg
      901 atcaggcctt ccttttggat gaacaggtac gtaagctcca tccagctgaa cgtggcttgg
      961 atacaggctt ctacgaattt gaccgcaata aggttaaata ataatattta tagcagaatt
     1021 tattta
//
LOCUS       DBNFCK010000216         1003 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.220, whole genome shotgun sequence.
ACCESSION   DBNFCK010000216 DBNFCK010000000
VERSION     DBNFCK010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.220"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..195)
                     /locus_tag="AC1PIW_001947"
     CDS             complement(<1..195)
                     /locus_tag="AC1PIW_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HIP6606829.1"
                     /translation="MKLIKLNHAVRVRFNPYIITSKRLFLSRVGMDLFLGMLLINTWV
                     SPYSIPIKSVLSLIFIGIICS"
     gene            complement(152..388)
                     /locus_tag="AC1PIW_001948"
     CDS             complement(152..388)
                     /locus_tag="AC1PIW_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606830.1"
                     /translation="MKIYKFMFNITYPALVISLYIIFQMKGTMSPWFDTLFVAGGFLV
                     IPLLLLLLGYLSENDHGGTDNEANQAESCSTSEV"
     gene            complement(443..763)
                     /locus_tag="AC1PIW_001949"
     CDS             complement(443..763)
                     /locus_tag="AC1PIW_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606831.1"
                     /translation="MKTFKDIFLSEGMEMPNINGIKRVQSFNSDKSVNFTLDDESRDF
                     LKENLPIEGVIYEPTLKKLAENIIILNRQKHRISDEFRISLMNKEIYQGYRETSFYTS
                     IIEA"
BASE COUNT          343 a          180 c          137 g          343 t
ORIGIN      
        1 agaacaaatt attccaataa atatcaatga aagcactgat ttaataggga ttgagtatgg
       61 agaaacccaa gtgtttatca ataacattcc taaaaataaa tccattccga ctctgctcaa
      121 aaacaatctt ttagaggtta ttatataagg attaaacctc actcgtactg catgattcag
      181 cttgattagc ttcattatct gtccctccgt ggtcattttc tgataaataa cccaacaata
      241 acaataaaag tggaataact aaaaaacctc cagctacaaa caaagtatca aaccacggag
      301 acatcgtccc tttcatctgg aatataatat acaaagaaat aactagagca ggataggtta
      361 tgttgaacat aaatttataa attttcataa taatttcctt tcataaaacc aaagggggag
      421 gggttagctc ccccttatat cactaagcct caattattga ggtgtaaaaa gaagtttccc
      481 tgtagccttg gtaaatctct ttgttcatca agctaatcct aaattcatca gaaatacggt
      541 gtttttgacg attaagaata atgatgtttt ctgctaactt cttcaaagta ggctcataga
      601 ttaccccttc aattggcaaa ttttccttta aaaaatctct ggattcatca tctaaggtaa
      661 agtttactga tttgtctgaa ttaaagctct gtactcgctt aattccattg atatttggca
      721 tttccattcc ttcgctaagg aaaatatctt taaatgtttt catctattta tttctccatt
      781 ttttattttg ttgttacagt caatattctt aaataaattg gtgaagctct agccttcatt
      841 agtattctcc tctttgtgtt tatgtataaa tgtagtatca gtcataaaca acagtaatta
      901 agctacagtt cctcaatgtt accatgagaa actgtaatcc tctactgcat tatttcttta
      961 tagtaagcaa aaataacaag ttttagaaca tatgacaatc taa
//
LOCUS       DBNFCK010000217          986 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.221, whole genome shotgun sequence.
ACCESSION   DBNFCK010000217 DBNFCK010000000
VERSION     DBNFCK010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.221"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            250..933
                     /locus_tag="AC1PIW_001950"
     CDS             250..933
                     /locus_tag="AC1PIW_001950"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HIP6606832.1"
                     /translation="MHYKHLLFDLDHTLLDFSRGEEVALTQFLTAMEVEDIQAFKEVY
                     RPLNQSMWKDLEKGNITKKELINTRFSRTFAHFGRQVDGREMALRYQEFIGRQGQIFT
                     GADKLLQELTHRGYQIYAATNGVTYIQENRLLHSPIQSYFKEVFISEQMGTQKPATDF
                     YEKIAEQIAGFQFAQALMIGDSLTADIQGGNNAGMDTVWYNPTHMINNSKAVPTYTIH
                     SYQELLDLL"
BASE COUNT          337 a          162 c          203 g          284 t
ORIGIN      
        1 tagttgaatt caaaaacttt atcaataaga tgtctatact tgaatactta gttgtgctat
       61 ctatcaagat aatacacact gatataaggg ctatctgccg ataaaataca gaaagccaag
      121 atatatctga ggctaagcgg tttaaaaagg agcggttctt aaaaatgaat tttacgatga
      181 tcactataga ttagaaacga attgatttca ctcaaataga gggtgttttt agagaggaaa
      241 atcccaaaat tgcactacaa acatttatta ttcgatcttg accatactct tcttgatttt
      301 agccgtggag aagaagtagc cttaacccag tttttgacag ctatggaggt tgaagatatt
      361 caagctttta aagaagttta tcgaccgctc aaccagagca tgtggaagga tttggaaaaa
      421 gggaacatta caaagaaaga gctaataaac acccgctttt cacggacctt tgcccacttt
      481 ggtcggcaag ttgatggaag ggagatggcc ttgcgctacc aagagtttat tgggcggcag
      541 ggacaaattt ttacaggtgc agataagctt ttgcaggaat tgacacaccg aggctaccag
      601 atttatgcag cgaccaacgg cgtgacctat atacaggaaa atcgcttgct tcactcaccg
      661 attcaatcgt attttaaaga agtatttatt tctgagcaga tggggacgca aaagcctgcg
      721 actgattttt atgaaaaaat agcagagcag attgcaggtt ttcaatttgc ccaagcctta
      781 atgattggag acagtttgac agcagatatt caaggcggaa ataatgctgg tatggatact
      841 gtttggtata atccgactca catgataaat aatagtaagg cagttccaac ctataccatt
      901 catagctatc aagaattatt agatttgtta taagatattt taagaaactt ataaggattt
      961 tagtatataa ttatacagaa agaata
//
LOCUS       DBNFCK010000218          982 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.222, whole genome shotgun sequence.
ACCESSION   DBNFCK010000218 DBNFCK010000000
VERSION     DBNFCK010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.222"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..>982)
                     /locus_tag="AC1PIW_001951"
     CDS             complement(<1..>982)
                     /locus_tag="AC1PIW_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606833.1"
                     /translation="QVNLDSVDNEIKALVARYINENNDLISPNEDFTSFFKTNLKAIN
                     LKFTHFREPDDDAPRTTTFLELIDFIFGNSLYEINKENLRFFMRWFKGSTDWSEEDFK
                     HKNFELLHTNDNYSPLLEYSRLKITDYVYAYLEICEGTILDNSEYIVELLQRNEVYES
                     GFNLGTLEEERQIDRILQFLPDNSINFNSDDFRQLVEEDILEEGNYLTLLSLLVQTSK
                     AVVDTDIILSYFTENQNYDNSLINFINNARDLKFEEDIFNLLEKEEQRNFFTKTVECM
                     ELDDTKYELILSTLGWHYTDTFNKSGIESSKLRILIDLEIIQFNSKNIEFF"
BASE COUNT          320 a          179 c          134 g          349 t
ORIGIN      
        1 gaaaaattca atgttcttag agttaaactg tataatttcc aaatcaatta atattctaag
       61 cttacttgac tctattccac ttttattaaa tgtatcggta tagtgccaac caagtgtgct
      121 aagtatgagt tcatatttgg tgtcatctaa ctccatacac tcaacagttt tggtgaaaaa
      181 gtttctttgt tcttctttct caagtaagtt aaatatatcc tcttcaaatt ttaaatctct
      241 tgcattattg ataaagttga taagactatt atcataattc tgattctcgg taaaataaga
      301 taagataata tcagtatcca ctacggcttt agaagtttga actagtaaac ttagcaaggt
      361 aagataattc ccctcttcaa gaatatcctc ttctacaagt tgacggaaat catcgctatt
      421 gaaattaata ctattatccg gaaggaactg taaaattcta tcaatttggc gttcttcctc
      481 cagtgttccc aaattaaatc cagactcgta aacttcgttt ctttgaagta gttctacaat
      541 atattccgag ttatctaaaa tagtcccctc acaaatctct aaatatgcat agacatagtc
      601 tgttatcttc aatctactat actctagcag cggtgaataa ttatcatttg tatgaagcag
      661 ttcaaaattc ttgtgcttga agtcttcctc tgaccagtca gtacttccct taaaccaccg
      721 cataaaaaat cttaaatttt ctttatttat ctcatacaaa ctattgccaa agataaagtc
      781 gataagctcc aagaaggtcg tagttctggg agcatcatca tcaggctctc taaaatgagt
      841 gaatttaaga ttgattgcct taaggtttgt tttgaaaaat gatgtaaaat cttcatttgg
      901 tgaaattaaa tcattatttt cattgatata acgagctact aacgccttta tttcattatc
      961 aacactatct aagttcacct ga
//
LOCUS       DBNFCK010000219          906 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.223, whole genome shotgun sequence.
ACCESSION   DBNFCK010000219 DBNFCK010000000
VERSION     DBNFCK010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.223"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            66..>906
                     /locus_tag="AC1PIW_001952"
     CDS             66..>906
                     /locus_tag="AC1PIW_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIP6606834.1"
                     /translation="MPNFASLHDIITGINSGIFKEEGLEVELIEFADGPTIIASLESG
                     SIDIGNIGPGAHKLAIEGRAEVVAFSQLGNADELIARSDRGIDSIEDLRGKKIGTASG
                     TSAETILKLALLEADLSEKDVEIIDMDASAIVTAMLSGSIDVAATWSPNTTFIKRELK
                     EKVVVLADNSRYREQSPAIASYVTFPGYSEKNNEKISKFLKGLYRAMDYRAEHMKEVS
                     KWVATQIGSDITAVQDQLQDGEWLTSRQMIQLINTGILQQYYEKQQKNLIESEIVDTS
                     RNIN"
BASE COUNT          309 a          135 c          196 g          266 t
ORIGIN      
        1 gtagttctat tcaaacctcc tctagtggcc attcagcaaa agttgacaca attagaattg
       61 catatatgcc aaattttgca agtcttcatg acattataac tggaataaat tcaggtatct
      121 ttaaagagga ggggcttgaa gtcgaattga ttgaatttgc tgatggtccg actatcattg
      181 catctttaga gtcaggttca attgatattg gtaatattgg tccgggtgcc cataaattgg
      241 cgattgaagg tagagcagaa gttgttgcat ttagtcaact tgggaatgct gacgaattga
      301 ttgctcggtc ggatagagga attgattcaa tagaggattt gagaggaaag aaaattggaa
      361 cagcatctgg gacatcagca gaaacaattc taaagcttgc tttgttagaa gcagatttgt
      421 ctgaaaagga tgtagaaatt attgatatgg atgcctccgc tattgttaca gcaatgttgt
      481 cgggttcaat tgatgtagct gcaacctggt caccaaatac aacttttata aagcgagaat
      541 taaaggaaaa ggtcgttgta ctagctgata attcacgtta tagagaacaa tctccagcta
      601 ttgcttcata cgtaactttt ccggggtatt ctgaaaaaaa taatgaaaaa atttccaagt
      661 ttttaaaagg gttatatcgt gcaatggatt atcgagctga acatatgaag gaagtttcaa
      721 aatgggttgc tacccaaatc ggttcagata tcacagctgt tcaagatcaa ctacaagatg
      781 gagagtggtt aacaagtaga caaatgattc aattgattaa cactgggata cttcaacagt
      841 attacgagaa acaacaaaaa aatttaatag aatctgaaat agttgataca tccaggaaca
      901 ttaatg
//
LOCUS       DBNFCK010000220          861 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.224, whole genome shotgun sequence.
ACCESSION   DBNFCK010000220 DBNFCK010000000
VERSION     DBNFCK010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.224"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(10..834)
                     /locus_tag="AC1PIW_001953"
     CDS             complement(10..834)
                     /locus_tag="AC1PIW_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIP6606835.1"
                     /translation="MTWVTNQSVVLQALLGGLFTWFCTIVGSAVVFFFKTVSRRLLDT
                     MLGFAAGVMIAASFWSLLAPSIEYAESSYGNLAWIPAAVGFAAGGIFLRLVDAWVPHL
                     HLGNDKDKAEGGGEKDRKNLSKTALLFLAITIHNIPEGLAVGVIFGALASNYSPAAFI
                     GAIGLAIGIGIQNIPEGAALAIPIRTDGASRWKAFYWGSMSAIVEPIAAVIGAFAVTF
                     MTPILPYALSFAAGAMIFVVVEELIPESQTNGNTDIATLGLMAGFIIMMILDVALG"
BASE COUNT          290 a          215 c          154 g          202 t
ORIGIN      
        1 tttaacaaat tatcctagag caacatctaa aatcatcatg atgataaaac cagccattaa
       61 gcccagagtt gcaatatccg tattgccgtt tgtctgcgat tctggaatga gctcttctac
      121 gacgacaaaa atcatggccc ctgccgcaaa ggagagggca taaggtaaaa taggtgtcat
      181 aaaagtgacc gcaaaagcac cgatgactgc tgcaatcggc tcgacaattg ctgacattga
      241 tccccagtaa aaagctttcc acctagatgc gccgtctgta cgaattggaa tagccaaggc
      301 tgccccttca ggaatatttt gtataccaat cccaatagca agaccaattg ctccgataaa
      361 agctgctgga ctatagttag aagcaagtgc accaaatatc acaccgaccg ccaaaccttc
      421 aggaatattg tgaattgtaa tagctaagaa gagcagggct gttttggata gattttttct
      481 atccttctca ccaccacctt ctgccttatc tttatcattt cccaaatgca aatgcggaac
      541 ccaggcatct accaagcgta aaaaaatacc acctgctgca aaacctactg ctgctggtat
      601 ccaagccaga ttgccgtatg aactttcagc atactcaatc gatggggcta ataaagacca
      661 aaaagaagcc gcaatcataa caccagctgc aaagcctaac atggtgtcta gcaaacgacg
      721 actaactgtc ttaaagaaaa atacaacggc agaccccaca atcgtacaaa accatgtgaa
      781 taaaccacct agcaaagcct gtagaactac agactgatta gtaacccaag tcataactaa
      841 catcctatcc tgtttaatct t
//
LOCUS       DBNFCK010000221          841 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.225, whole genome shotgun sequence.
ACCESSION   DBNFCK010000221 DBNFCK010000000
VERSION     DBNFCK010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.225"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..744
                     /locus_tag="AC1PIW_001954"
     CDS             <1..744
                     /locus_tag="AC1PIW_001954"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIP6606836.1"
                     /translation="IRPTKPLKELTWIDLIIITTILCGNAIYTSTMQWIASFSATETV
                     ETGVLSFSPADNWWALANQGKLFLFALVYLLIRNYDFKQLKVKLEWTVLLWGPLIFIG
                     AGLISDLTFTAFSYIPGLSGGYNFLGYLPYYDWNIMTVLNRFLAVDYSTVIYSLFNGF
                     YEEFFFLGLLLSTDKKKRSLVLLFSTIVRISFHTYQGMVSALVIGVAFGLFYYYMYTR
                     KNDNLLPYFLGHALADMVGTSFFSLFIAG"
BASE COUNT          230 a          161 c          161 g          289 t
ORIGIN      
        1 attcgtccaa caaagcccct aaaagaactc acttggatag atttgattat tatcacaacg
       61 attttatgtg gtaatgctat ttatacctca accatgcaat ggatagcatc attttctgca
      121 acagaaaccg ttgaaacagg agttctgtcg tttagtccag ccgataattg gtgggctcta
      181 gccaatcaag gaaaattatt cctgtttgcc ctggtgtatt tattgattcg gaattatgat
      241 ttcaagcagc taaaagtaaa attagaatgg accgttctac tctggggacc actgattttc
      301 attggtgcgg gcctgattag cgatttgaca tttacagctt tttcatatat tccaggtctg
      361 tctggtgggt ataatttcct cggatacttg ccttactatg actggaatat catgacagtg
      421 ctcaaccgtt tcctagccgt tgactacagc acagttatct attccttgtt taatggcttc
      481 tatgaagagt tctttttctt aggattattg ttgagcacag ataagaaaaa gcgttcgctg
      541 gtcttactct tttcaacaat tgtccgtata tctttccata cctatcaagg aatggtatct
      601 gctcttgtta ttggtgtagc cttcggcctt ttctattact acatgtacac aaggaaaaat
      661 gacaatttat tgccatattt cttaggacat gctcttgcgg atatggttgg tacaagtttc
      721 ttctcattat ttattgcagg gtaaacaatg agaagaacgg acatcaagct aatcggataa
      781 aatttctttc tccctacagc tgacaaacgg atgatgatac gtagtttcta ttaacttggt
      841 a
//
LOCUS       DBNFCK010000222          840 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.226, whole genome shotgun sequence.
ACCESSION   DBNFCK010000222 DBNFCK010000000
VERSION     DBNFCK010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.226"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(52..837)
                     /locus_tag="AC1PIW_001955"
     CDS             complement(52..837)
                     /locus_tag="AC1PIW_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIP6606837.1"
                     /translation="MVLLATMTLSACSGNATQEDTSLKDVQEKGKLIVALNPEFPPFE
                     FRTLVDGKDTIVGADIELAKAIGQELGVEVEFSAMSFDNVLNNVQSGQSDIAISGISA
                     TEERAKVYDFSIPYYTSTNKVIINKEDLAQYTSLDSLAEANIGAQKGSIQEQIIKEQL
                     PSSTVIALASNGELINQLKSQKVEAVIFEEPIAKAYVAKNDDLVILDTEIKSSYSDAY
                     AIALPKGSTALKEKVDSVITKLVESGQMDQFVQEAYELSIANN"
BASE COUNT          250 a          169 c          133 g          288 t
ORIGIN      
        1 tatacatttg tcaagaaaat attattttta ttcactttaa ttgtataaaa tttaattatt
       61 agcaatggat aattcatagg cttcttgaac aaattgatcc atctgtccag attccactag
      121 ttttgtaatg acagagtcaa ctttttcctt taaagctgta cttccttttg gcaaggcaat
      181 agcataggca tctgaatagc tactttttat ctcagtatct aaaataacca agtcatcatt
      241 tttggcaaca taagctttcg caatcggctc ttcaaaaata actgcttcta ccttctgtga
      301 ttttaattga ttaattagct cgccgttcga agctagagca ataaccgtgg aggacggaag
      361 ctgttcttta ataatttgtt cttgaataga acctttttgt gctcctatat ttgcctcagc
      421 tagagaatca agcgatgtat attgtgcaag atcctcttta tttataataa ctttattggt
      481 tgaggtgtag taaggaatgg aaaaatcata cacttttgca cgctcttcag tggctgaaat
      541 tcctgaaatg gcaatatccg attgccctga ctggacattg ttcaaaacat tatcaaaact
      601 catcgctgaa aattcgactt ctacacccaa ttcttgacca atggcttttg caagctcaat
      661 gtcagctcca acgattgtat cttttccatc aaccagtgtt ctaaactcaa atggtggaaa
      721 ttctggattc aatgcaacaa ttagttttcc cttttcctga acatccttca aggaagtatc
      781 ttcttgagta gcatttccac tgcaagcact tagggtcatc gttgccaaca aaaccaatcc
//
LOCUS       DBNFCK010000223          833 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.227, whole genome shotgun sequence.
ACCESSION   DBNFCK010000223 DBNFCK010000000
VERSION     DBNFCK010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.227"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..128
                     /locus_tag="AC1PIW_001956"
     CDS             <1..128
                     /locus_tag="AC1PIW_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HIP6606838.1"
                     /translation="GLNEKTVAKYIREQEKNDIALDKLSVKEYEDSFSDSSFRTR"
     gene            complement(268..>833)
                     /locus_tag="AC1PIW_001957"
     CDS             complement(268..>833)
                     /locus_tag="AC1PIW_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HIP6606839.1"
                     /translation="VLSILTLIGTAIFLFWLYKIGILNDQNVLSDLIKSQGALGSLSF
                     LAIQIIQVVFPIIPGGVTTVVGFLVFHFWWGFFLNYLGISIGSIILFWLARRYGKKFC
                     LLFMSEDTFYKYESKIDNKRSYEIFFILCMLSPISPADIVVMITGLTSMSYRKFIMIT
                     LLCRPFSVVAYSFFWIYGSQWLQQLLG"
BASE COUNT          310 a          152 c          164 g          207 t
ORIGIN      
        1 ttggactgaa cgaaaagacg gtagcgaaat atattcgcga gcaggagaaa aatgacatcg
       61 cacttgataa gttaagtgtt aaagaatatg aagattcatt ttcagatagt agttttagaa
      121 caagataaaa gcccgttgtt acgggcatta gtcaagtaat tatagcacta acctgaacga
      181 agttcagcga acgtctttag acgtcgctgg agagaaacgg cttatagccg gtgtacaagc
      241 cacccgtttt cacgggtggt tatgatttta tcctaacaat tgctgcaacc attggctgcc
      301 atagatccaa aagaagctat aggccacaac tgagaaaggg cgacaaagta aagtaatcat
      361 aataaatttc cgataactca tgcttgttaa tccagtaatc atcaccacta tgtcagctgg
      421 agaaatggga gaaagcatgc agagaataaa gaaaatttca taactacgtt tattatcaat
      481 tttgctttca tatttgtaaa acgtatcttc agacatgaag aggagacaaa atttctttcc
      541 atagcgacga gccaaccaga aaaggatgat actaccgatt gaaattccaa gataattgag
      601 aaagaatccc caccaaaaat gaaatacgag gaaacccaca acagtcgtaa caccgcctgg
      661 aataatcgga aatacaactt gaataatctg aatagctaaa aaggagaggc tacctaaagc
      721 accttggctt ttgattaaat cgcttaagac attttggtcg ttcaaaatgc caatcttata
      781 gagccaaaat agaaaaatag cggtcccgat aagggtcaaa atacttaata ctt
//
LOCUS       DBNFCK010000224          820 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.228, whole genome shotgun sequence.
ACCESSION   DBNFCK010000224 DBNFCK010000000
VERSION     DBNFCK010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.228"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..804)
                     /locus_tag="AC1PIW_001958"
     CDS             complement(<1..804)
                     /locus_tag="AC1PIW_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIP6606840.1"
                     /translation="MILSKRFKNRRKELGFTQKELAEGICEQSLISRVEKLGVAPTSD
                     ILFALSQRLQVSMDYFFDESVSDKAPDITVFKRLVDKALFTRSYDQLAYLVEAEKQKE
                     AVHSQESSEYLTYLACIVDFHHYHKEDIAIGCMEELSHRISKKSSFYLDVYNSLVNFY
                     ALASRDEDLDGLYEGISEKLSHLDISNTECFHKYIKIRYNHAHYLFKRKRQSQAIDEL
                     TDLIETLRDKKSCYFLADMLCLIANVGEGFLSKDEILSYYREAECLFKFF"
BASE COUNT          264 a          179 c          115 g          262 t
ORIGIN      
        1 aaaaaatttg aatagacatt ctgcttctct atagtaggag agaatttcat cttttgatag
       61 aaatccctct ccgacatttg cgattaaaca aagcatatct gccaaaaaat aacaactctt
      121 cttatctcgc aatgtttcaa ttaggtctgt caattcatca attgcctgtg actgcctctt
      181 ccttttaaaa agatagtgag cgtgattgta gcgaatcttg atatacttat gaaaacactc
      241 tgtgtttgat atatccaagt ggctaagctt ttctgaaatc ccctcataca aaccatctaa
      301 atcctcatct cgagaagcca aggcataaaa attaaccaag ctgttgtata catccagata
      361 aaaactagat ttcttactga ttcgatgact aagttcttcc atacatccaa ttgcaatatc
      421 ctccttatga taatgatgaa agtccactat acaagccaga taagttaaat attcactgct
      481 ttcctgcgaa tgaacagctt ctttctgctt ctctgcctct acaagataag ccagttgatc
      541 ataggaacga gtgaacagag ctttgtccac caatcgttta aataccgtaa tatcaggcgc
      601 tttatctgat acactttcat caaaaaaata gtccatagac acctgtaagc gttgtgataa
      661 ggcaaataat atatccgatg taggagcaac tcctaatttt tcaactcgac taatcaaact
      721 ctgctcacaa attccctctg caagctcctt ttgagtaaag ccaagttcct ttcttctatt
      781 tttaaaacgt ttactaagaa tcattttcat aacctttctg
//
LOCUS       DBNFCK010000225          815 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.229, whole genome shotgun sequence.
ACCESSION   DBNFCK010000225 DBNFCK010000000
VERSION     DBNFCK010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.229"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..466
                     /locus_tag="AC1PIW_001959"
     CDS             <1..466
                     /locus_tag="AC1PIW_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIP6606841.1"
                     /translation="LFRPMITQLAIYRRAGDYNQFKQYQKRIVKMVVGLAVLVLVGGI
                     VLGIPALNILYGTNLNKYWLSFIITMLGGIASTFATICDNMLTVLRKQKYLVISFAIS
                     CLLSILISNPLVEYYGILGAAIAFVSSMWTWFLISLVIYLKLQKHLNWEVQE"
     gene            463..>815
                     /locus_tag="AC1PIW_001960"
     CDS             463..>815
                     /locus_tag="AC1PIW_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HIP6606842.1"
                     /translation="MKIEKLFLRLAIPFLLISIFVMPVTKVPDEATHAFMSWNILFDS
                     PTSRDTEAMNELRAADFTYSEPEIHAVDSTEYSSLFTKIRDFSKDKLSIDFSLKSLMS
                     TPQLIGLVVGKLLYPS"
BASE COUNT          244 a          115 c          161 g          295 t
ORIGIN      
        1 tttgtttaga cccatgataa ctcagttggc tatatataga agagctgggg actataatca
       61 atttaaacaa tatcagaagc gtatcgttaa aatggttgtc ggattggctg tgttagtttt
      121 ggttggggga atagtattgg ggattccagc attaaatatt ctctacggaa cgaatcttaa
      181 taaatactgg ctcagcttta ttataacaat gcttggggga attgcgagta cctttgcaac
      241 catttgtgat aacatgctta ctgttttgag gaagcaaaag tatctcgtaa tttcatttgc
      301 aatttcttgt ttattatcca tattgatttc taatccattg gtagaatatt atggaatatt
      361 gggggcagct attgcttttg ttagttcgat gtggacttgg tttttaattt ctttagtaat
      421 atatctaaaa ttgcaaaaac atttgaattg ggaagtacaa gaatgaagat agaaaaatta
      481 tttttaaggt tagcaatccc ctttttgtta atttcaatat ttgttatgcc tgtgacaaag
      541 gttcctgatg aagcaacaca tgcttttatg tcgtggaata tattatttga ttctccgaca
      601 agtagagata ctgaggcaat gaatgaatta cgggcagcag attttacgta ttcagaaccg
      661 gagattcatg cagttgattc tacagaatat tcaagccttt ttaccaaaat tcgtgatttt
      721 tcaaaggaca aattatctat cgatttttct ctgaaatcgt taatgagtac acctcagcta
      781 attggtctag ttgtaggtaa attgctttat ccgtc
//
LOCUS       DBNFCK010000226          759 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.230, whole genome shotgun sequence.
ACCESSION   DBNFCK010000226 DBNFCK010000000
VERSION     DBNFCK010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.230"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            28..657
                     /locus_tag="AC1PIW_001961"
     CDS             28..657
                     /locus_tag="AC1PIW_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HIP6606843.1"
                     /translation="MIKEFCSENHVGVMKALSLGAKRIELCDNLAVGGTTPSYGVVKH
                     VCQLAHENEATVMTMIRPRGGDFCYDKAEVEMMVEDCKAAIELGADGLVYGLLTEENW
                     LDEPALERLFAVSQDCQIVFHMAFDQIPRERQFEALDWLVEHGVTRILMRGSLTGSAL
                     NNVEWLQEIVAYAQGKIEILIGGGLTVDNVPTLLEALPINQVHGTRLFW"
BASE COUNT          229 a          111 c          204 g          215 t
ORIGIN      
        1 taaagaaaaa acttgaaagg aataaagatg attaaagaat tttgttccga aaatcatgta
       61 ggtgtgatga aggccctatc cctaggagct aaacggattg agctctgtga taatttggca
      121 gtcggaggga cgacgccgag ttatggagtg gtcaaacatg tttgtcaact ggcccatgag
      181 aatgaagcca cagtcatgac catgattcgc cctcgcggtg gagatttttg ctacgataaa
      241 gcagaggttg aaatgatggt tgaagattgt aaagcagcga ttgaactggg ggcagatggt
      301 ctggtttatg gtttgttaac cgaggaaaat tggttggacg aaccagcctt ggaaagactg
      361 tttgctgttt cgcaggactg tcagattgtc tttcacatgg cttttgacca aattcctcgc
      421 gaacgccaat tcgaggctct tgattggctg gtagaacatg gagtgacacg gattttgatg
      481 cgtgggagtc tgacagggtc tgccttgaac aatgtggaat ggttacagga aattgtcgcc
      541 tatgcacaag gtaaaattga gattttgatt ggtggaggat tgactgtgga caatgtgcca
      601 acgttattag aagcattacc aatcaatcaa gtacatggaa cgagattatt ttggtaaggt
      661 agctttgaaa gtgaaataaa ttacagagta ataatcgaag tttattcaga aatgtttaga
      721 agttaaggtt tgtttaagca ttggaagata caatatatt
//
LOCUS       DBNFCK010000227          734 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.231, whole genome shotgun sequence.
ACCESSION   DBNFCK010000227 DBNFCK010000000
VERSION     DBNFCK010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.231"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            141..>734
                     /locus_tag="AC1PIW_001962"
     CDS             141..>734
                     /locus_tag="AC1PIW_001962"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014735676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HIP6606844.1"
                     /translation="MNILFTLNDAFVPQVAACMGSIMRTLDEDDTCHFYLFSDGISQQ
                     NKENLHQFVTDGGNKLTIVELENLESYFDFEVDTNGWASVVLARLLVDKLLPEEVDRI
                     IYLDGDTLVLENIRELWEVDLEGKVLGMCPEPTASSERREGLNLGTYTYHNAGVLLID
                     LKRWRSKSIGTIIFDYYKEKNGELFANDQDALNGALKE"
BASE COUNT          246 a           92 c          169 g          227 t
ORIGIN      
        1 aaaagagcag gtaagtgtat cggtaaaaca gaagtatttg atacggttgc tcactggcac
       61 agtagtcatt aactttatca tgtatctatt tttgatagtg gattattatt gggtgtaaga
      121 agatagtgga agagggaaac gtgaatatat tatttacatt gaatgatgct tttgttccgc
      181 aagttgcagc ttgtatgggc tcaatcatgc gaacattaga cgaagacgat acatgccatt
      241 tttatctttt ctcagatggt ataagtcaac agaataaaga aaaccttcat caatttgtta
      301 ccgacggagg aaataaattg acaatcgttg aattggaaaa cttggaatca tattttgatt
      361 ttgaagttga cacaaatggt tgggcttcgg ttgtacttgc aagattactt gtagataaat
      421 tactgcccga agaagttgat agaatcatct atttagatgg agatacttta gttttagaaa
      481 atattcggga gctatgggaa gtagatttgg aggggaaagt ccttggaatg tgcccagaac
      541 cgacagcttc atctgagaga agagaaggct taaatttggg tacgtataca tatcataatg
      601 cgggagttct tctaatcgat ttgaaacggt ggcgttcaaa aagtatagga acaattattt
      661 ttgattatta taaagaaaaa aatggagagc tttttgctaa tgatcaagat gcactgaatg
      721 gagcattgaa agaa
//
LOCUS       DBNFCK010000228          733 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.232, whole genome shotgun sequence.
ACCESSION   DBNFCK010000228 DBNFCK010000000
VERSION     DBNFCK010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.232"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(105..178)
                     /locus_tag="AC1PIW_001963"
     tRNA            complement(105..178)
                     /locus_tag="AC1PIW_001963"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(142..144),aa:Arg,seq:acg)
     gene            complement(197..280)
                     /locus_tag="AC1PIW_001964"
     tRNA            complement(197..280)
                     /locus_tag="AC1PIW_001964"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(244..246),aa:Leu,seq:taa)
     gene            complement(288..359)
                     /locus_tag="AC1PIW_001965"
     tRNA            complement(288..359)
                     /locus_tag="AC1PIW_001965"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Gly,seq:gcc)
     gene            complement(372..444)
                     /locus_tag="AC1PIW_001966"
     tRNA            complement(372..444)
                     /locus_tag="AC1PIW_001966"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(409..411),aa:Thr,seq:tgt)
     gene            complement(459..540)
                     /locus_tag="AC1PIW_001967"
     tRNA            complement(459..540)
                     /locus_tag="AC1PIW_001967"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(504..506),aa:Leu,seq:tag)
     gene            complement(544..616)
                     /locus_tag="AC1PIW_001968"
     tRNA            complement(544..616)
                     /locus_tag="AC1PIW_001968"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(581..583),aa:Lys,seq:ttt)
     gene            complement(662..733)
                     /locus_tag="AC1PIW_001969"
     tRNA            complement(662..733)
                     /locus_tag="AC1PIW_001969"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(699..701),aa:Asp,seq:gtc)
BASE COUNT          189 a          217 c          154 g          173 t
ORIGIN      
        1 gaacctgcga caccttggtc ccaaaccaag tactctacca agctgagcta cttcccgata
       61 ctattaaaaa taaagctccc ctaacgtctc cattaagcga gtgatgcacc ctagaggatt
      121 cgaacctcta accgcctgat tcgtagtcag gtactctatc cagttgagct aagggtgcta
      181 actattataa tcttcatgcc gaggaccgga atcgaaccgg tacgatgttg ccatcgcagg
      241 attttaagtc ctgtgcgtct gccagttccg ccaccccggc ctctcaaagc gaacgacggg
      301 attcgaaccc gcgaccccca ccttggcaag gtggtgttct accactgaac tacgttcgca
      361 atctttccta atgccggcta catgacttga acacgcgacc ctctgattac aaatcagatg
      421 ctctaccaac tgagctaagc cggcctattt ccattctatg cgggttaagg gacttgaacc
      481 cccacgcccg aaagcgccag atcctaaatc tggtgcgtct gccaattccg ccaaacccgc
      541 atatgactcg tgctgggctc gaaccagcga cccattgatt aaaagtcaat tgctctacca
      601 actgagctaa cgagtcttta cttaattata ttacttgaaa catatgattg attcgtttca
      661 acggtcccga cgggaatcga acccgcgatc ttcgccgtga cagggcgacg tgataaccgc
      721 tacactacgg gac
//
LOCUS       DBNFCK010000229          726 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.233, whole genome shotgun sequence.
ACCESSION   DBNFCK010000229 DBNFCK010000000
VERSION     DBNFCK010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.233"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..>726
                     /locus_tag="AC1PIW_001970"
     CDS             <1..>726
                     /locus_tag="AC1PIW_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HIP6606845.1"
                     /translation="SFLYKDNDRSINDANKALKFFNDVDDEIEQYYTLGSLLGLLLVS
                     SNYERANQVKQEIETLSDKHNLPLYWKSKNNFVVLDFLSGMEGDFDYWKSRFESILTE
                     YELNDVSKHLMYTNLCAISLYYSKTKGYRSYKTILEELMDVEDLADLEDTSIDDFYRY
                     YFGWFEFCLLLLESKHRQAKNKYNQLKDFSPIIFNSNKKLLIEKHRRYKKIFESNIKT
                     GKEFSEFLSQSKFASREWNYFRR"
BASE COUNT          274 a           95 c          117 g          240 t
ORIGIN      
        1 aaagtttttt atacaaggat aatgatagat caatcaatga tgccaataaa gctttaaaat
       61 tttttaacga tgtcgatgat gaaattgaac agtattatac acttggtagt cttctaggat
      121 tattacttgt ttcttccaac tatgagcgag caaatcaggt gaaacaagaa atagaaacac
      181 tatcagataa acacaatctc ccactatact ggaaatctaa aaataatttt gttgtactgg
      241 actttctttc aggtatggaa ggagattttg attattggaa atctcggttt gaaagcattc
      301 ttactgaata tgagttaaat gatgtgagta aacacttaat gtatacaaat ttatgtgcaa
      361 tttctctata ttattctaaa acaaaagggt acagaagcta taaaacgata cttgaagaat
      421 tgatggatgt tgaggactta gccgaccttg aagatacctc aatagatgat ttttatcgtt
      481 attattttgg atggtttgaa ttttgcttgt tgttactaga gagtaagcat cgacaagcaa
      541 aaaataaata caatcaatta aaagatttct cacctattat tttcaattca aataaaaaat
      601 tattaattga aaaacatcgt cgctataaaa agattttcga atcaaatata aaaacaggaa
      661 aggaattttc tgaatttcta tcacaatcca agtttgcttc aagagaatgg aattactttc
      721 gaagag
//
LOCUS       DBNFCK010000230          684 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.234, whole genome shotgun sequence.
ACCESSION   DBNFCK010000230 DBNFCK010000000
VERSION     DBNFCK010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.234"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..240
                     /locus_tag="AC1PIW_001971"
     CDS             <1..240
                     /locus_tag="AC1PIW_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HIP6606846.1"
                     /translation="IEMLMSNQSAGTIMKNISQTTLSEIRIVIPNKEIQKVFSNEVRS
                     LLDVIENNLKQNQELTQLRDWLLPMLMNGQVKVEE"
     gene            261..>684
                     /locus_tag="AC1PIW_001972"
     CDS             261..>684
                     /locus_tag="AC1PIW_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606847.1"
                     /translation="MSELSKVTIRISGKAMDESKGYELKYLLKSLDSFSKLSEKTYLH
                     MISEDRLKKSESGNFALYIDNFQHGSFLADLVIVMNSYALPLLEHGQTIWGAITTVYD
                     FIKTISSAKEEGKSVSIENVGDGSIAVAVSDSSSVGDIT"
BASE COUNT          243 a           95 c          136 g          210 t
ORIGIN      
        1 attgaaatgc tcatgtctaa tcaatctgcg ggaactataa tgaaaaatat ttctcaaact
       61 acactttcag aaattcgcat tgtgatccca aataaagaaa ttcagaaggt attttcaaat
      121 gaagtcagaa gtcttttaga tgtaattgaa aataatttaa aacaaaacca agaactgacc
      181 caactccgtg attggctctt gccaatgctg atgaatgggc aggtgaaggt agaggagtaa
      241 cgaaaataga ggtttatgct atgagtgaat taagcaaggt cacgatcaga atttctggta
      301 aagcaatgga tgaatctaaa ggttacgaac ttaaatattt gttaaaatct ttggatagtt
      361 tttccaagtt atcagaaaag acgtatttac atatgataag cgaagatagg ctcaaaaaga
      421 gtgaaagtgg taattttgca ctatatattg acaattttca acatggttct ttcttagctg
      481 atttagtaat tgtaatgaat tcatatgccc ttcctttact tgaacatggt caaactattt
      541 ggggagctat cacaactgtt tatgatttta taaaaacaat ttcctctgcc aaagaagaag
      601 gtaaatcggt aagtattgag aatgtcggtg atggttcaat agccgtagcg gtaagtgaca
      661 gttcaagtgt tggagatatt acaa
//
LOCUS       DBNFCK010000231          647 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.235, whole genome shotgun sequence.
ACCESSION   DBNFCK010000231 DBNFCK010000000
VERSION     DBNFCK010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.235"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            64..>647
                     /locus_tag="AC1PIW_001973"
     CDS             64..>647
                     /locus_tag="AC1PIW_001973"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6606848.1"
                     /translation="MKLLKKTSSLNLIDFEQADTYNLIENIVQDSTYKPFQLFNAIIG
                     VITGFLSLLTSIIYVSTWNVAVSTFLLIIPVISLVIFMRVGQLEFLIQWERANSEREV
                     WYINYLLTHDFSFKEIKLNGISQYFIDKYGTLKRNFMNQDLNISKKKVLFRGSLDILL
                     NFINGIAIFMMIQSIRSGKLLVGNFVSLIQAITRV"
BASE COUNT          217 a           79 c          107 g          244 t
ORIGIN      
        1 ctaagtcagt taaactcata tataagtgag aaatttaata tgaacttgtc ctataatctc
       61 aatttgaagt tattgaaaaa aacatcatct ctaaatttaa tagattttga acaagcagat
      121 acatacaatc tcattgaaaa tattgtacaa gatagtacat ataagccgtt ccaacttttc
      181 aatgctatca ttggtgtgat tacagggttt ctatctttat taacaagtat tatttatgtg
      241 tccacttgga atgtggcagt atcgactttt ttactgataa ttcctgttat ttccttagta
      301 atttttatgc gtgttggcca gctagaattt ttgattcaat gggagcgagc aaattcggag
      361 agggaagttt ggtacatcaa ttatcttctt actcatgatt tttcatttaa agagattaaa
      421 ctaaatggta ttagtcaata tttcattgat aaatatggaa cattgaaaag aaattttatg
      481 aatcaggatt tgaatatttc gaaaaagaaa gttcttttca gaggaagttt ggatatttta
      541 ttgaatttta ttaatggtat agctattttt atgatgatac agtcaattcg aagcggtaaa
      601 cttttagtag gtaattttgt aagccttatt caagcaataa ctcgtgt
//
LOCUS       DBNFCK010000232          641 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.236, whole genome shotgun sequence.
ACCESSION   DBNFCK010000232 DBNFCK010000000
VERSION     DBNFCK010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.236"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            25..641
                     /locus_tag="AC1PIW_001974"
                     /pseudo
     CDS             25..641
                     /locus_tag="AC1PIW_001974"
                     /inference="COORDINATES: protein motif:HMM:NF019371.6"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
BASE COUNT          240 a           99 c          111 g          191 t
ORIGIN      
        1 tcaaaaattg tgacagttga tggagtgcgt caaataaatt accaaaacac cccattcaaa
       61 aaaattactg ttagatatta tcaagataaa cgattgcgtc tccatctatt ggatgctctc
      121 gaagacattg aggtagcttt aaaacccaag tagcatttgt tttaggttat aggagattag
      181 ggccatatgg atatttaaat ttctcacagt ggtgcaataa aaaagagtac tgcaagcatt
      241 acctaaaata tcgagaagag aaatttaaaa agcagctcaa agctgaattg aaaagaacaa
      301 cttctccaga aatcaaggaa aaattgaaaa ttgacaatat gaaatatcct ccaatttggt
      361 tggctattaa cctaatgacg ttcggtcaga taatcaacct tatcgaattg atgtcaacaa
      421 ataatattag acaaatagct aaacaatatg attgcagtga tgctgagtta ctttcttggt
      481 tgaaatgttt gaatttatta cgtaatatgt gtgctcataa ttcgaatatt attgatctta
      541 aaatgcatac aacaccaata cttcgtgaag aatggaagga gctcttatac gaactaaaag
      601 aaggtgttta ttcaaaccgc attgctctgc cgattattat a
//
LOCUS       DBNFCK010000233          610 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.237, whole genome shotgun sequence.
ACCESSION   DBNFCK010000233 DBNFCK010000000
VERSION     DBNFCK010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.237"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..>610)
                     /locus_tag="AC1PIW_001975"
     CDS             complement(<1..>610)
                     /locus_tag="AC1PIW_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HIP6606849.1"
                     /translation="GLIGSFFLYAHLIQNELGSTKNDGTIEIIAEQPNQVIDSIQING
                     RYIHSSEIIENQWGINDADLIPNFSSLGEENSLVIKSSSSVRTLSFEYFVSENPTIVK
                     IKVGGQLVESIDTSTGDKYKNLAFIELPYTLRITKDNQFWYLHLIVLALGLTCFILNG
                     ATWRIKRRDIRILTILLIVQYIFTTFTFPRLYRNELVLFNSN"
BASE COUNT          202 a           87 c          106 g          215 t
ORIGIN      
        1 aagttcgaat taaatagcac taattcattg cgatataaac gtggaaatgt aaaagtagtg
       61 aatatatatt gaacgattaa taatattgtt aagatacgga tatctcttct tttgattctc
      121 catgttgctc catttaagat aaagcatgtt agacctaaag caaggactat taagtggagg
      181 taccaaaatt gattatcttt agttatccgt aaagtatagg gaagctcaat aaaggctaaa
      241 tttttatatt tatctccagt tgatgtgtca atggattcaa ccaattgtcc accgacttta
      301 atttttacaa tagtcggatt ttcactaaca aagtattcaa atgataaggt tcttacagaa
      361 gaggaagatt taattacgag tgaattttcc tcacctagag aagaaaaatt gggaattaaa
      421 tcagcgtcat tgattcccca ttggttttct ataatttctg atgaatgaat ataacgtcca
      481 tttatttgga tgctgtcgat tacttggttt ggttgttctg cgattatttc tatagttcca
      541 tcattttttg tagaacctaa ttcgttttga atgaggtgtg catagagaaa aaaacttcca
      601 atcaatccga
//
LOCUS       DBNFCK010000234          608 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.238, whole genome shotgun sequence.
ACCESSION   DBNFCK010000234 DBNFCK010000000
VERSION     DBNFCK010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.238"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..>608
                     /locus_tag="AC1PIW_001976"
     CDS             <1..>608
                     /locus_tag="AC1PIW_001976"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIP6606850.1"
                     /translation="TSMDNGAEKSIYYLMKYLIQLGHTVINVTHAIGGEPQDKHEQLY
                     KMIGVKNYYLPTIKWWWPDAPGGEILNREEATSYYRQNVQDIAEIIEQNEIDLVISNT
                     VNVFQGAIAASCQKVPHFWLIHEFPKNEFAYYADKIDFIEEYSSELFSVTGELNNFLT
                     PLFNKKVHSFISYTEQETKVLKKGNQVRIVSVGRLTEGKNQL"
BASE COUNT          216 a           85 c          119 g          188 t
ORIGIN      
        1 actagtatgg ataatggtgc ggagaagtct atttattatc taatgaagta ccttattcaa
       61 ctgggacaca cagttataaa tgttactcat gcaataggag gggaaccaca agataagcat
      121 gagcaacttt ataaaatgat aggggttaag aattactatt tgccaactat taagtggtgg
      181 tggccggatg caccaggtgg agaaatcctt aatagagaag aggccacaag ctactatcgc
      241 cagaatgttc aagacatagc tgaaattatt gagcaaaatg agatagactt ggttatttca
      301 aatactgtta atgtttttca aggagcaatt gctgcctcat gtcaaaaggt tcctcatttc
      361 tggctaattc atgaatttcc taaaaatgag tttgcttatt atgctgataa aattgacttc
      421 atagaagagt attcttcaga attatttagt gtgactggag agttaaataa ttttttaaca
      481 ccactattta ataagaaagt ccattcgttt atttcttata ctgagcaaga aacaaaagtt
      541 ttaaaaaaag gcaatcaagt tagaattgtt tctgttggtc gattaacaga aggaaaaaat
      601 caattaga
//
LOCUS       DBNFCK010000235          605 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.239, whole genome shotgun sequence.
ACCESSION   DBNFCK010000235 DBNFCK010000000
VERSION     DBNFCK010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.239"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(248..592)
                     /locus_tag="AC1PIW_001977"
     CDS             complement(248..592)
                     /locus_tag="AC1PIW_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606851.1"
                     /translation="MKQANSKLVALTLITTLAVTSVIATTVHAEEKVTADQTVAGKHK
                     PITGEQLQKAIGDRNPNSIQIQAKFENTNFKNQGITFIGKAEIPSKSSEKSSWFWELW
                     TRFTTFVKGIFS"
BASE COUNT          190 a          116 c           89 g          210 t
ORIGIN      
        1 aacaattaca tcctaaactt ttcactcttt aatttatcta agaaaaatta caactgagac
       61 tcaagaaata tggaattcgt gtcaacatct cattcgcaaa aaatgtgagg tctattacat
      121 aatttcttgc acaatctcta gtttagatgc ctagatctct ccaatatttc atacgctttt
      181 catttgaata aaagctcaca tgaaccactg ttctctatcc atatgaagtc aacgaaaaat
      241 agaaacctta gctaaaaata cctttgacaa aagttgtaaa acgagtccaa agttcccaaa
      301 accatgaaga cttttcagaa gatttagatg gaatttcagc ttttccaata aaagttattc
      361 cttgattttt gaaattggta ttttcaaatt tagcttgtat ctgaatacta tttggatttc
      421 tatctccaat cgctttttga agctgctcac ccgtaatcgg tttgtgtttt cctgctacag
      481 tctgatcagc agttactttc tcttcagcat gcaccgttgt cgcaatcaca gaggtaactg
      541 cgagagttgt aatgagtgtt aaagctacta gttttgaatt agcttgtttc atgaaatgtc
      601 tcctt
//
LOCUS       DBNFCK010000236          598 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.240, whole genome shotgun sequence.
ACCESSION   DBNFCK010000236 DBNFCK010000000
VERSION     DBNFCK010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.240"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..487
                     /locus_tag="AC1PIW_001978"
     CDS             <1..487
                     /locus_tag="AC1PIW_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIP6606852.1"
                     /translation="HEQEEKANFFQNIKSEKNSGIIWGIKLISTDLLMVLLGVPVWIV
                     VGVEFNRLSYFAYVGVITWLLLVLLNHFHMLLSLIMGKGGNLVISFIECLFIIFATNK
                     VFLNNFWLPIVLPVNMILEIGKNEIFMILVYLIGFIILSYFCNLAVMNNVEIQKICKK
                     R"
BASE COUNT          204 a           65 c          106 g          223 t
ORIGIN      
        1 tcatgaacaa gaggaaaaag caaatttctt tcaaaatatt aaaagcgaaa aaaatagtgg
       61 aattatttgg ggaataaagc ttatttcgac agacttactt atggttttat tgggagttcc
      121 agtttggata gtggtaggag ttgaatttaa tagactctct tactttgcgt atgttggtgt
      181 gattacttgg ttattattag tcttacttaa ccatttccat atgttactta gtcttatcat
      241 gggaaaggga gggaatctag taatttcttt tatcgaatgt ctgtttatta tatttgcaac
      301 caataaagta tttttaaata acttctggtt gcctatcgtt ttacctgtta atatgatcct
      361 agaaattggg aaaaatgaaa tttttatgat tctagtatac ttaattggtt ttatcatatt
      421 atcgtatttt tgtaatttag ctgttatgaa taatgttgaa attcaaaaaa tatgtaaaaa
      481 gaggtaaatt ggttagaggt cactaaaaga gtagaataca acaaaattca atagataaaa
      541 agcgaatgac agcatatttt gaaatctaaa gattgcagat catccgcttt ttatgtct
//
LOCUS       DBNFCK010000237          596 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.241, whole genome shotgun sequence.
ACCESSION   DBNFCK010000237 DBNFCK010000000
VERSION     DBNFCK010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.241"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..407)
                     /locus_tag="AC1PIW_001979"
     CDS             complement(<1..407)
                     /locus_tag="AC1PIW_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NisI/SpaI family lantibiotic immunity
                     lipoprotein"
                     /protein_id="HIP6606853.1"
                     /translation="MRKILFSLVVLIGICTLVACRLVDNKTIEFHDQSFNQFTYKGND
                     YTIMNQIVDENDLGTVKESFFKTLVIDRNSQKVVTKSNSDTVSLAYSGLYNFSDGLAI
                     SINDSYYKVSLSSDVQSNNEMLSLEEFNNNSAGG"
     gene            complement(409..>596)
                     /locus_tag="AC1PIW_001980"
     CDS             complement(409..>596)
                     /locus_tag="AC1PIW_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lanthionine synthetase C family protein"
                     /protein_id="HIP6606854.1"
                     /translation="DMTYGFNTTAEFENIKTKDNLGYLDGIIGILLTMIELNNLKVTT
                     NWQRALLLFDDVIKEVK"
BASE COUNT          205 a          112 c           59 g          220 t
ORIGIN      
        1 ccacctgctg aattattatt gaactcttcc aagctcagca tttcattatt ggattgcaca
       61 tccgaactca aagaaacctt ataataagaa tcatttatag aaatagctaa accatcacta
      121 aaattataga gcccactata tgctaatgat actgtatcac tattactttt agtaacaact
      181 ttctggctat ttctatcaat tactaatgtt ttaaaaaaag attccttcac agtacctaga
      241 tcattttcat caacaatttg attcatgatt gtataatcat ttcctttata tgtaaattga
      301 ttaaatgatt gatcatgaaa ctctattgtt ttattgtcta ccaatctaca tgcaactaaa
      361 gtacaaattc caataagaac aactaagcta aataaaattt tcctcatgct acttcacctc
      421 ctttataaca tcatcaaata ataagagtgc cctttgccaa tttgttgtaa cctttaagtt
      481 atttagttcg atcattgtta acagaattcc aattattcca tctaaataac caagattatc
      541 ttttgttttt atattttcaa attccgcggt agtattaaaa ccatatgtca tgtctt
//
LOCUS       DBNFCK010000238          591 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.242, whole genome shotgun sequence.
ACCESSION   DBNFCK010000238 DBNFCK010000000
VERSION     DBNFCK010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.242"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..>591)
                     /locus_tag="AC1PIW_001981"
     CDS             complement(<1..>591)
                     /locus_tag="AC1PIW_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009909570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase Wzy"
                     /protein_id="HIP6606855.1"
                     /translation="GATMFTGAVEGIGFSQGFNGGLTHKNFFGITILMGFVLTYLAYK
                     YGSYKRTDRFILGLELFLILISNTRSVYLILLLFLFLVNLDKIKIEQRQWSTLKYISM
                     LFCAIFLYYFFGFLITHSDSYAHRVNGLINFFEYYRNDWFHLMFGAADLAYGDLTLDY
                     AIRVRRVLGWNGTLEMPLLSIMLKNGFIGLVGYGIVL"
BASE COUNT          234 a          125 c           71 g          161 t
ORIGIN      
        1 taaaacaatc ccatacccta ccagaccgat aaaaccattt tttaacataa tactcagtaa
       61 gggcatttca agcgttccat tccaacctaa aacgcgtcta acccttatag catagtctaa
      121 agttaaatcc ccatatgcca aatccgctgc accaaacatt agatggaacc aatcatttct
      181 ataatactca aaaaaattaa taagaccatt aacgcgatga gcgtaagaat cactatgtgt
      241 tattaaaaaa ccaaagaaat agtataaaaa aatagcacaa aatagcatgg aaatatattt
      301 aagcgtactc cattgtcttt gttctatttt gattttgtca agattaacaa gaaatagaaa
      361 aagcaatagt attaaataaa ctgagcgtgt gtttgaaata agaatcaaaa acaattctaa
      421 tcctaaaata aaacgatccg ttcttttata ggaaccatac ttatacgcca agtaagttaa
      481 tacgaacccc attaaaatag ttattccaaa aaagttctta tgcgtcaatc ctccattaaa
      541 accctgacta aaaccgatac cttctactgc ccccgtgaac atcgttgccc c
//
LOCUS       DBNFCK010000239          590 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.243, whole genome shotgun sequence.
ACCESSION   DBNFCK010000239 DBNFCK010000000
VERSION     DBNFCK010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.243"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..>590)
                     /locus_tag="AC1PIW_001982"
     CDS             complement(<1..>590)
                     /locus_tag="AC1PIW_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIP6606856.1"
                     /translation="IQLVTETLDKFSYLEFGALGIAVPGHFDSKSGHIISNNVKWIYF
                     DLAVIKQSISIPIFLENNINCMAIGSYLFHPESSPEQFLFIHIGPGLFCSFFDSEHIL
                     QNKNFYIGEIGHTVVDLNGPSCECGKRGCLQTYISDTWLINNARFLFENVQGSIIKTL
                     VEKPEDITLDVVYNAYRLGDGFIIEKIESGIDFLTT"
BASE COUNT          223 a           98 c           82 g          187 t
ORIGIN      
        1 ggtagttaaa aaatcaatcc ctgattcaat tttttcaata ataaaaccat ctcctagtcg
       61 gtatgcattg tatacaacat ctaatgtaat atcttctggt ttttcaacca aagtttttat
      121 gatagaacct tgaacattct caaataaaaa tcttgcatta ttgattaacc aagtatcaga
      181 aatataagtt tgaaggcatc cacgttttcc acattcacaa gaaggtccgt ttaaatcaac
      241 tactgtatga ccaatctctc caatataaaa gtttttgttt tgtaaaatat gttcactatc
      301 aaaaaatgaa cagaacaatc caggtccaat atgaataaat aaaaactgct ctggcgaact
      361 ttcgggatga aagagatatg aaccaattgc catgcaatta atattgttct ctagaaaaat
      421 aggaattgaa atactttgtt taattacggc aagatcaaaa taaatccact ttacattatt
      481 tgaaataata tgtcctgatt ttgaatcaaa gtgaccaggt acagcaatac cgagagctcc
      541 aaattcaaga taggaaaact tatctagagt ttcagttact aattggataa
//
LOCUS       DBNFCK010000240          582 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.244, whole genome shotgun sequence.
ACCESSION   DBNFCK010000240 DBNFCK010000000
VERSION     DBNFCK010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.244"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..483)
                     /locus_tag="AC1PIW_001983"
     CDS             complement(<1..483)
                     /locus_tag="AC1PIW_001983"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002936347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIP6606857.1"
                     /translation="MEKVSIIVPIFNTEKYLRECLDSIISQSYTNLEILLIDDGSSDS
                     STDICLEYAEQDGRIKLFRLPNGGVSNARNYGIKNSTANYIMFVDSDDIVDGNIVESL
                     YTCLKENDSDLSGGLLATFDGNYQESELQKCQIDLEEIKEVRDLGNENFPNHYMSGIF
                     N"
BASE COUNT          186 a          126 c           69 g          201 t
ORIGIN      
        1 attaaagata ccgctcatat aatgatttgg aaaattttca tttcctaagt ctcgcacctc
       61 ttttatctct tccaaatcaa tttgacactt ttgcagctca gattcttgat aatttccatc
      121 aaaagtagca agtaaccctc ccgacaaatc actatcattc tcttttaaac aggtgtataa
      181 ggactcaaca atgttgccgt caacaatatc atcagaatct acaaacataa tataatttgc
      241 tgtgctattt ttgataccgt aattccttgc gtttgaaaca ccaccatttg gtaaccggaa
      301 aagttttatt ctaccatctt gctctgcgta ttccaaacat atatccgttg atgaatctga
      361 agaaccgtca tctatcaaaa gaatctctag attagtatac gattgggaaa taatgctatc
      421 taaacactct cttaagtact tttccgtatt aaaaataggt acaataatgc tgactttttc
      481 catcctctaa ttccttttat gccgtaactt gctttgctat cataaaaatc atcatgtaaa
      541 aaatactatc ttctatcttg ccacctatag aacatatgct ct
//
LOCUS       DBNFCK010000241          576 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.245, whole genome shotgun sequence.
ACCESSION   DBNFCK010000241 DBNFCK010000000
VERSION     DBNFCK010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.245"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..514
                     /locus_tag="AC1PIW_001984"
     CDS             <1..514
                     /locus_tag="AC1PIW_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606858.1"
                     /translation="KYNLLEKEAKKHEYKKEALDFNKVDWKTVSNRYIELKQIVIDEK
                     IKDINQDEIERYYLANTENFERQDFISGNISIWNDGIIISTESVDITEETVRNITERY
                     PDLELELKNIEVGHQVSWGQNGVYYTFTCLSREEKGIIPLSEITDAVAMQCAEELVDN
                     WLNKEIDSLK"
BASE COUNT          231 a           58 c          106 g          181 t
ORIGIN      
        1 aaaatataat cttttagaaa aagaagcgaa aaaacatgaa tataaaaaag aggctcttga
       61 tttcaataaa gttgattgga agacagtctc aaatcgctat attgaattaa aacaaattgt
      121 tattgatgaa aaaattaaag atataaatca agatgagatt gaacgatatt acttagcaaa
      181 tactgaaaat tttgaacgtc aagattttat aagcggtaac atttctatat ggaatgatgg
      241 gataattatt tctactgaat cagtagatat taccgaggaa acagtaagaa atataactga
      301 aagataccct gatttagaat tagaattaaa gaatattgaa gttggtcatc aagtttcttg
      361 gggacagaat ggggtttatt atacctttac atgtttgtcg agagaagaaa agggaattat
      421 tccactttct gaaatcacgg atgcggttgc tatgcaatgt gcagaggaac ttgttgataa
      481 ttggttaaat aaagaaattg actctttaaa ataatccttg attattgaca atctagagtt
      541 aacaaaaatt ttttgttaat ttaaaaggaa ttggga
//
LOCUS       DBNFCK010000242          552 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.246, whole genome shotgun sequence.
ACCESSION   DBNFCK010000242 DBNFCK010000000
VERSION     DBNFCK010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.246"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..>552
                     /locus_tag="AC1PIW_001985"
     CDS             <1..>552
                     /locus_tag="AC1PIW_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serum opacification factor"
                     /protein_id="HIP6606859.1"
                     /translation="ALPTNTLSYIGKKIGLTNTSEKAIDLFANSLVDYIFYGRKNVFY
                     NHNLSAQAEAKIATEKGIHFYAVDVTKDKAGEEKNKFDDYLKKMSGDKGEFLNIDENV
                     AEKFKDVLTKVSLVDSLENDVEFAKKPDSSKVKITNGTSSLLWSFFSNSKTTITWNLD
                     KEDLIKAYQTNKPLSLTYQLQHKK"
BASE COUNT          205 a           87 c           98 g          162 t
ORIGIN      
        1 gcattgccta caaatactct cagttatatc ggtaaaaaga ttggtttgac aaatacctct
       61 gaaaaagcaa ttgacttgtt tgcaaattcc ctcgttgatt acattttcta cggtcgaaaa
      121 aatgtctttt acaatcataa cctctctgct caggcagaag caaagattgc tactgagaaa
      181 gggattcatt tctacgctgt tgatgtgacc aaagacaaag ctggcgaaga aaagaataaa
      241 tttgatgact acttgaaaaa aatgtctggg gataagggcg agtttttaaa tattgacgag
      301 aatgttgcag aaaaatttaa agatgtattg actaaagtga gcttagtaga ttcgcttgaa
      361 aatgatgttg agttcgcgaa aaaaccagat agttctaaag taaaaatcac aaatggtaca
      421 tctagtttat tatggtcgtt tttctctaac tcgaaaacaa ctattacttg gaacttagat
      481 aaagaagact taatcaaggc ttatcaaact aacaagccac tatctttaac atatcaattg
      541 caacataaaa aa
//
LOCUS       DBNFCK010000243          538 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.247, whole genome shotgun sequence.
ACCESSION   DBNFCK010000243 DBNFCK010000000
VERSION     DBNFCK010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.247"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            136..516
                     /locus_tag="AC1PIW_001986"
     CDS             136..516
                     /locus_tag="AC1PIW_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606860.1"
                     /translation="MSFINQFKSQSKTVLATVAISVAILGFAGGMSISKSSAGVSTQS
                     QVTLAQNGSVAKSGQVATQGMLPGQGGATQDISPGQGITPPDATSGATQSNSSTSSDE
                     VNALKKRNQEIKAQISGDSSSSNQ"
BASE COUNT          178 a           87 c          108 g          165 t
ORIGIN      
        1 taatggactt ttataaaaaa tgctgaattt ctttagcttg ttttaagttt tcaatactat
       61 actattatca tcaggtgaag aggagttatt taacaggtct cattctgata atattctatt
      121 gaaaagggga atttcatgtc atttattaat caatttaagt cacagtcaaa aacagtacta
      181 gctactgttg ctatttcagt tgctatcttg ggctttgcag ggggaatgag tatttctaaa
      241 agctcagcag gagtttcaac acaaagtcag gtaactttgg cgcaaaatgg ttccgtagct
      301 aagagtggac aagtagctac ccaaggcatg ctaccaggtc agggaggagc tacacaagat
      361 atatcaccgg gacaaggtat aactcctcct gatgcgacaa gtggagcaac tcagtcaaac
      421 tcgagtactt cttctgatga agtgaatgct ttaaaaaaga ggaatcaaga aattaaagct
      481 caaatttcag gagatagtag ttcttctaat caataaatat tgagatttcg atttttac
//
LOCUS       DBNFCK010000244          536 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.248, whole genome shotgun sequence.
ACCESSION   DBNFCK010000244 DBNFCK010000000
VERSION     DBNFCK010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.248"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..>536
                     /locus_tag="AC1PIW_001987"
     CDS             <1..>536
                     /locus_tag="AC1PIW_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606861.1"
                     /translation="LTENDKVYYLGVSKIRFMLDNSVWSQLTRAKLFHIILLVSVLIP
                     LFSKSNIEFNYQLQNIEYLWQTCYIISIVVYIFLLKLSVSIVYGVFRLNLENVSKTVK
                     KEDVKEKDSRHKEKIFNRFGITDKINISVQRSFKDEFWKIYKNQNQYRDDYIKVRLQE
                     RITLVEKADRDELIFTIF"
BASE COUNT          198 a           50 c           98 g          190 t
ORIGIN      
        1 tttaactgaa aatgataaag tttattactt aggtgttagt aagattcgct tcatgctaga
       61 taattcagtg tggtcgcagc taaccagagc taaattattt catattattc tattagtgtc
      121 ggtattaata ccgttgttca gcaaaagtaa tatagaattt aattatcagc ttcagaatat
      181 agaatatctg tggcaaacct gttacattat ttctatcgtg gtttatatct ttttgctgaa
      241 acttagtgtc agtattgtat atggggtttt tagattaaat ttagaaaatg taagtaagac
      301 ggttaaaaag gaagatgtta aggaaaaaga tagtagacat aaagaaaaaa tttttaatag
      361 atttggtatc actgataaaa ttaatatctc agtacaaagg tcctttaagg atgagttttg
      421 gaaaatttat aaaaatcaaa atcaatatag ggatgattat ataaaagtaa gacttcaaga
      481 gagaattaca ttggttgaga aagctgatag ggatgaatta atttttacta tttttg
//
LOCUS       DBNFCK010000245          513 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.249, whole genome shotgun sequence.
ACCESSION   DBNFCK010000245 DBNFCK010000000
VERSION     DBNFCK010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.249"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            68..>513
                     /locus_tag="AC1PIW_001988"
     CDS             68..>513
                     /locus_tag="AC1PIW_001988"
                     /inference="COORDINATES: protein motif:HMM:NF017000.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="HIP6606862.1"
                     /translation="MSKTESKLYPLGCLNFGLAHGLAGVGAMLSYSKLKGYSNEKSIA
                     AIKKIIMLYEKHELKNYMWKEGLSDIELKKTEKSNLQYEFIRDAWCYGSPGISLLYLY
                     SSLALEDKKLKSKACNILKASIRRSNGLEQSILCHGFSGAIEICLF"
BASE COUNT          181 a           72 c           97 g          163 t
ORIGIN      
        1 tttcttatct gagttttcac taaaaaattc tactttaaca ggcttctatg tggaatcaaa
       61 gaatcaaatg tccaagacag agtctaagct ttatccgtta ggatgtctaa attttggttt
      121 agcacacggc cttgcgggtg ttggcgcaat gttgtcttat tcaaaattaa aaggctattc
      181 taatgaaaaa tctattgcag caatcaaaaa aattataatg ttatacgaaa aacacgagtt
      241 aaaaaattat atgtggaaag agggattaag cgatattgaa ctaaagaaaa cagaaaaaag
      301 caatttacag tatgaattta ttcgagatgc gtggtgctac ggtagcccag gaattagttt
      361 actatatctg tattcaagtt tggctctaga agataagaaa ctaaaatcta aagcttgtaa
      421 catcttgaaa gcatccatca ggagaagtaa tggtttggaa caatctattt tatgtcacgg
      481 attttctgga gcaattgaga tttgcctatt ttt
//
LOCUS       DBNFCK010000246          510 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.250, whole genome shotgun sequence.
ACCESSION   DBNFCK010000246 DBNFCK010000000
VERSION     DBNFCK010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.250"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(72..159)
                     /locus_tag="AC1PIW_001989"
     tRNA            complement(72..159)
                     /locus_tag="AC1PIW_001989"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(123..125),aa:Ser,seq:gga)
     gene            complement(162..>510)
                     /gene="ssrA"
                     /locus_tag="AC1PIW_001990"
     tmRNA           complement(162..>510)
                     /gene="ssrA"
                     /locus_tag="AC1PIW_001990"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          146 a          124 c           92 g          148 t
ORIGIN      
        1 tttcatttgt tttcagttgt atcattcaca gacaaaaaaa gaacgactat cagtcgttct
       61 tcaataacat acggaagaca tgggattcga acccacgcac gctgttacac gcctaccgcg
      121 tttccaacac ggcctcttga gcctcttgag taatcttcca tatatggagc tggtgggagt
      181 cgaacccacg tccaaacacc tgctaactta ttcgtctaca accataggtt atgtcttgct
      241 ttaactttaa cttgaactcg atacataact caaacctaga ccaagcgagt cagtaaatct
      301 cttttggaac tgcctgactc aatcccaacg tagcttgcta ttttaagacc aatcagcaaa
      361 cacaagcaat tcgcgactgg tcacgcaggc aggttattaa gctgctaatg cgtaagtgtt
      421 tgtatttttt gcagttatat ttaactggca tttttacatc tgccgatgag ttgcagaaca
      481 agcctcataa tgcctgtcga atccgtaaca
//
LOCUS       DBNFCK010000247          503 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.251, whole genome shotgun sequence.
ACCESSION   DBNFCK010000247 DBNFCK010000000
VERSION     DBNFCK010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.251"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(136..>503)
                     /locus_tag="AC1PIW_001991"
     CDS             complement(136..>503)
                     /locus_tag="AC1PIW_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIP6606863.1"
                     /translation="IVNESHTRVIKGNRLNLDRALINILSNATRFCSEEGKVVLTISE
                     SSNFISFEIWNNGLPFTEESLKDGGKLFYTENKGRNDKHYGIGLSFAQKIAMRHQGQL
                     ILSNPQNGGAQVTLLIKKF"
BASE COUNT          160 a           88 c           71 g          184 t
ORIGIN      
        1 cagaaaaaaa ttgagcaaaa agtcgatatc tctttgattt tttcaaattt tcgaaataaa
       61 aaatcgcata aaatcaacgt ttttaaatga aagatttcat gcggttttgt ttatattagg
      121 ctttttgacc agcccctaaa attttttaat taataaagtg acctgtgcac caccattttg
      181 tggatttgag agtattaatt gaccttgatg tctcatagct atcttttgag caaatgaaag
      241 tcctattccg taatgcttat catttcttcc tttattctct gtgtaaaaaa gttttcctcc
      301 atctttaaga gactcttctg taaatggtaa accattattc caaatctcaa aactgataaa
      361 gttggaactt tcagatatag tcagtaccac cttgccctct tctgaacaaa atctagtcgc
      421 attagataag atatttatca aagctcgatc taagttgaga cgatttcctt taatcactct
      481 ggtatgggat tcattaacta ttt
//
LOCUS       DBNFCK010000248          502 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.252, whole genome shotgun sequence.
ACCESSION   DBNFCK010000248 DBNFCK010000000
VERSION     DBNFCK010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.252"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..312)
                     /locus_tag="AC1PIW_001992"
     CDS             complement(<1..312)
                     /locus_tag="AC1PIW_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SSU0592/SSU0593 family protein"
                     /protein_id="HIP6606864.1"
                     /translation="MKQLVTKRSYKIGLSAMALVLLMTTAGGATAHASTGTYSVETRG
                     DAKVYRLLETPAGSFTQGGIVLKPVNNSTTKPSETKSWFDSFWSSVKTFWEAICRGVG
                     WC"
BASE COUNT          177 a          113 c           71 g          141 t
ORIGIN      
        1 acaccatcca actccacgac agattgcttc ccaaaatgtt tttacactac tccaaaaaga
       61 atcaaaccat gatttggttt cagatggttt agttgtagaa ttattcacag gtttcaaaac
      121 tatgcccccc tgtgtaaaag accccgccgg agtttccaac agcctataca ctttggcatc
      181 acctcgagtt tcgacactat aggtacccgt acttgcatgt gcagtggcac cacctgcagt
      241 ggtcattagc aagaccaaag ccatagcact caaaccaatt ttataactac gttttgttac
      301 aagttgcttc attgttgaaa tatctccttt atacgatatt cgtctctaca aatgatagta
      361 tagcacaaac agacaactca aattccaact ttagaaaaag gttagataaa aaactataaa
      421 actccaagat atgcatatta acacaagaac aaaaatacac atgcaatatt atatttatgg
      481 ttcaaaaaac ttgtatcgaa ta
//
LOCUS       DBNFCK010000249          478 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.253, whole genome shotgun sequence.
ACCESSION   DBNFCK010000249 DBNFCK010000000
VERSION     DBNFCK010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.253"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            162..>478
                     /locus_tag="AC1PIW_001993"
     CDS             162..>478
                     /locus_tag="AC1PIW_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIP6606865.1"
                     /translation="MGKKEETIRNISGNILKTLFRHQKSSRTRISKSLKITPATITNM
                     ISYLVAEKKVIETGDEVRDYIGSGRSRRLITVNSQYRHYIGIEINANGIYLAVTDTIG
                     NLIT"
BASE COUNT          185 a           59 c           70 g          164 t
ORIGIN      
        1 attaaagata tcatgtaagc gcttaattgt caagttttta tttataaatt ttaattaaaa
       61 aatgaaattt atttatttgt ttagtgtttt tacttcattt tttataaaaa atatgttatt
      121 cttacattga aaaatactat tttccaatgt gaggtactaa aatgggaaaa aaagaagaaa
      181 ccattcgaaa tatttctgga aatatattaa aaactttatt tagacatcaa aaaagctcta
      241 gaacaagaat ttctaaatct ttaaaaatta caccggcaac tattacgaat atgattagtt
      301 acctggtagc tgaaaaaaaa gttattgaga ctggcgatga ggttcgagat tatattggat
      361 caggtcgtag tagacgactt attacagtaa actctcaata tagacattat attggaatcg
      421 agattaatgc caatggaatc tatttagccg tcacagacac tattggaaac ctaatcac
//
LOCUS       DBNFCK010000250          438 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.254, whole genome shotgun sequence.
ACCESSION   DBNFCK010000250 DBNFCK010000000
VERSION     DBNFCK010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.254"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..>438)
                     /locus_tag="AC1PIW_001994"
     CDS             complement(<1..>438)
                     /locus_tag="AC1PIW_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HIP6606866.1"
                     /translation="SHWNQFLTEITYSIIQAHTDQKSLSLYIPFEDSLSKSLAFDTIS
                     QELLESEIDGNGTVLYSDFGDNNITDEYKLSFAEKFFKPISVSVNNSIQGFGTAGLSL
                     KYDSTEMEKKILNNSNNYRLIFLIQHWEWLGSELKLFFIRLLN"
BASE COUNT          141 a           69 c           67 g          161 t
ORIGIN      
        1 tattgagaag gcgtataaaa aataatttta gttcactccc tagccactcc caatgttgga
       61 ttagaaatat taatctgtaa ttgttgctat tatttagtat tttcttctcc atctcagttg
      121 aatcatattt taatgacaga ccagctgttc caaatccttg tatcgagtta ttaacagata
      181 ctgaaatagg cttgaaaaat ttttctgcaa atgaaagttt atactcgtct gtaatattgt
      241 tatctccgaa atcactatac aatactgtac cattcccgtc aatttcgctt tccaatagtt
      301 cctgtgatat tgtatcaaat gctaaggatt ttgataaaga atcttcgaac gggatatata
      361 gtgaaagtga cttttgatct gtatgtgctt gaataattga ataagtaatt tctgttaaaa
      421 attgattcca atgagaca
//
LOCUS       DBNFCK010000251          436 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.255, whole genome shotgun sequence.
ACCESSION   DBNFCK010000251 DBNFCK010000000
VERSION     DBNFCK010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.255"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..>436
                     /locus_tag="AC1PIW_001995"
     CDS             <1..>436
                     /locus_tag="AC1PIW_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HIP6606867.1"
                     /translation="EELEAMAKTLYDYWFVQFDFPDENGKPYKSSGGKMVYNDQLKRE
                     IPEGWGVKQLGEICEFRNGINYEKSETGDTLSKIVNVRNISNSSTFVTTHDLDSITLD
                     RRRIESYLVTDRTILITRSGIPGATRIVSDIPVNTIYSGFIIG"
BASE COUNT          135 a           65 c          103 g          133 t
ORIGIN      
        1 gaggagttgg aggctatggc caagacgctt tatgattatt ggtttgtgca gtttgatttc
       61 cctgatgaaa atggtaagcc ctacaagtcc tctggtggta aaatggtcta taacgaccaa
      121 ctcaaacggg aaatcccaga agggtgggga gtgaagcagt tgggtgagat atgtgaattt
      181 agaaatggaa tcaattatga aaaatctgag acaggggata cgctttcaaa aattgtaaac
      241 gtacggaaca tttcaaattc tagtactttt gttactactc atgatttgga ctcaattact
      301 ttagatagaa gacgtattga aagctatctt gttacagata gaacaattct gataactcgc
      361 agtggaattc ctggggcgac taggatagtc tctgatattc cagttaacac aatttattca
      421 ggttttatta ttggtg
//
LOCUS       DBNFCK010000252          422 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.256, whole genome shotgun sequence.
ACCESSION   DBNFCK010000252 DBNFCK010000000
VERSION     DBNFCK010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.256"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..>422)
                     /locus_tag="AC1PIW_001996"
     CDS             complement(<1..>422)
                     /locus_tag="AC1PIW_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnan synthesis F family protein"
                     /protein_id="HIP6606868.1"
                     /translation="NSHVSKDIVQYLQSERLIDFFIQRDNIGYDFAAWKEGLNQVTFY
                     QYDSVTLMNDTCFGPLWDLEDYYSQFDSDVDVDFWGMTNHLETKIDSVVVPEHLQSYF
                     MVFKKRILQSQAFVGFWSSVSELTDIQDVIKLYESQLT"
BASE COUNT          137 a           90 c           56 g          139 t
ORIGIN      
        1 tttgtcaatt ggctctcata taattttata acatcttgaa tatctgttaa ctcacttacc
       61 gatgaccaaa aaccaacaaa tgcttgactc tgcaagattc gttttttaaa taccataaaa
      121 tatgactgta aatgttccgg gacaactaca ctatctattt ttgtctctaa gtgattggtc
      181 atcccccaaa aatctacatc aacatcactg tcaaattgac tataataatc ctctaggtcc
      241 cagagggggc cgaaacatgt atcattcatc agagttaccg aatcatattg ataaaatgtt
      301 acttgattca gcccttcttt ccatgccgca aaatcataac caatattatc tctttggata
      361 aaaaaatcaa tcagtcgttc cgactgtaaa tattgtacta tgtcctttga aacatggctg
      421 tt
//
LOCUS       DBNFCK010000253          416 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.257, whole genome shotgun sequence.
ACCESSION   DBNFCK010000253 DBNFCK010000000
VERSION     DBNFCK010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.257"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..>416
                     /locus_tag="AC1PIW_001997"
     CDS             <1..>416
                     /locus_tag="AC1PIW_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001213956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Spaf_1101 family AAA-like ATPase"
                     /protein_id="HIP6606869.1"
                     /translation="DIFFKNKKIKVDKTKFFGSFDKVIFSSPKEYISFLLLAEAILKN
                     EIEIVVVTDHNTTKGIKKLQTAVSIITRTYPTYNIHPHILHGVEISAADKLHIVCIYD
                     HEKESWVNQWLCENIISEKDGSYQHSLTIMKDFNNQ"
BASE COUNT          168 a           48 c           62 g          138 t
ORIGIN      
        1 tgatatattt ttcaaaaata agaaaatcaa agttgataaa acaaaatttt ttggtagctt
       61 tgataaagtt atcttttcta gcccaaaaga gtacattagt tttcttttgt tagcagaagc
      121 tatcttaaaa aatgaaatag aaatagttgt agtaactgat cataatacta ccaaaggtat
      181 taaaaaactt caaacagcag tttcaattat aacgagaacc tatccgactt ataatataca
      241 tcctcatatt ttacatggag tagaaattag tgcagcagat aagttacata ttgtttgtat
      301 atacgatcat gaaaaagaat cttgggttaa tcaatggtta tgtgaaaata ttataagtga
      361 aaaagatgga agttatcagc attcgttgac tatcatgaaa gattttaata atcaaa
//
LOCUS       DBNFCK010000254          409 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.258, whole genome shotgun sequence.
ACCESSION   DBNFCK010000254 DBNFCK010000000
VERSION     DBNFCK010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.258"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..370)
                     /locus_tag="AC1PIW_001998"
     CDS             complement(<1..370)
                     /locus_tag="AC1PIW_001998"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606870.1"
                     /translation="MRNISSGRGAGFDFRLVNISNIYHHLQLKDFRNEEVLNFDLIEY
                     MLKNLELQSREENLSAILLQEDNLDFLVELAKKLYDSKSEKYNLEQFGQLMEFWLSTN
                     PQRFIDYMKSTKVEYQAPLKN"
BASE COUNT          136 a           84 c           44 g          145 t
ORIGIN      
        1 tattctttag tggagcttga tactccacct tcgtagactt catatagtca ataaatcttt
       61 gtggattagt agatagccaa aactccatca actgcccaaa ctgttccaag ttatattttt
      121 cagacttact atcatacaat ttctttgcca gttcaaccaa aaaatccaag ttatcttctt
      181 gtaataagat agcactcaag ttttcttctc gactctgcaa ctccaaattt ttaagcatgt
      241 attcaatcaa gtcaaaattt aaaacttctt catttctaaa gtcctttaat tgcaaatgat
      301 gatagatatt tgaaatattt accagtctga aatcaaaacc tgcacctctt ccactggaaa
      361 tatttctcaa aaattcttta tcattcttag ttaagacatt gccataaaa
//
LOCUS       DBNFCK010000255          401 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.259, whole genome shotgun sequence.
ACCESSION   DBNFCK010000255 DBNFCK010000000
VERSION     DBNFCK010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.259"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            62..397
                     /locus_tag="AC1PIW_001999"
     CDS             62..397
                     /locus_tag="AC1PIW_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SSU0592/SSU0593 family protein"
                     /protein_id="HIP6606871.1"
                     /translation="MKRIVTKRSYKVGLCAMALALLMSTAGGVTAGAQGHNFTSSDSS
                     AMRNTQVLVKGNTSNRSSHDGPVKIAMPRAAGSKKQKTWYESLWSTIKKFFEQLRHGF
                     HLERLFKNG"
BASE COUNT          132 a           59 c           88 g          122 t
ORIGIN      
        1 atatactggc actacattgt ctaaaaaagg gcgaatattg tataaaggag atatttcaaa
       61 aatgaaacgt attgtaacaa aacgtagtta taaagttggt ctgtgtgcta tggctcttgc
      121 tctgttgatg agcactgcag gtggcgtaac tgctggtgca caggggcata attttacttc
      181 atcagatagc tctgcaatga gaaatactca ggtgcttgtt aagggaaata catcaaatcg
      241 gagttctcat gatggtccag taaaaattgc gatgccgaga gctgctggtt ctaaaaaaca
      301 aaaaacttgg tatgaatctc tttggagtac cattaaaaag ttttttgaac aacttcgtca
      361 tggttttcat ttagaaagac tgtttaaaaa tggatagtaa t
//
LOCUS       DBNFCK010000256          382 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.260, whole genome shotgun sequence.
ACCESSION   DBNFCK010000256 DBNFCK010000000
VERSION     DBNFCK010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.260"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..>382
                     /locus_tag="AC1PIW_002000"
     CDS             <1..>382
                     /locus_tag="AC1PIW_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HIP6606872.1"
                     /translation="VIRLLREFTKNFRLIEKISLNMGIPGMDIGRKETAILECNNILL
                     TLYQKDEELTKLLEMEKKDKEDLSFQVAALAHDIKTPLTVLKGNIELLEMTNLDEKQL
                     DFMSSMNHSVGIFEKYFNSMVNYSR"
BASE COUNT          150 a           41 c           68 g          123 t
ORIGIN      
        1 tgttattagg ctcttaagag aatttacaaa aaattttcgt ctaattgaaa aaatatctct
       61 gaatatggga ataccaggaa tggatattgg acgtaaagaa actgcaatct tagaatgtaa
      121 taacatttta ttgacgcttt atcaaaaaga cgaagagttg acaaagttat tagaaatgga
      181 gaaaaaagat aaagaagatt tgtcatttca agttgctgca ttagcacatg atattaaaac
      241 accattaact gttttaaagg ggaatatcga gttgcttgag atgactaatc tagatgaaaa
      301 acaattagat tttatgtcct caatgaatca tagtgttggt atatttgaaa agtattttaa
      361 ttcaatggtt aattactcaa ga
//
LOCUS       DBNFCK010000257          365 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.261, whole genome shotgun sequence.
ACCESSION   DBNFCK010000257 DBNFCK010000000
VERSION     DBNFCK010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.261"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            39..>365
                     /locus_tag="AC1PIW_002001"
     CDS             39..>365
                     /locus_tag="AC1PIW_002001"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIP6606873.1"
                     /translation="MLALLDDDIISDDEKTNIADHILKISIKNKQYSEKLTAHILENQ
                     FDEEDLSYIISSQFFDNQSLSLKELVKQIAIENLDTIAEEFYAQISNNLMRKLIAESQ
                     LNTLKKL"
BASE COUNT          152 a           41 c           59 g          113 t
ORIGIN      
        1 atctagatac taaaagtaaa atatataagg aagatgagtt gttagcatta cttgatgatg
       61 acattatttc ggatgatgag aaaactaaca ttgcagacca tattttaaaa atcagtataa
      121 aaaataaaca gtattcagaa aaattgactg ctcatatttt ggaaaatcag tttgatgaag
      181 aggatttgag ttatatcatc agtagtcaat tctttgacaa ccagtcactg agtttgaaag
      241 agctagttaa acaaattgca attgaaaatc ttgatactat agctgaagaa ttttatgccc
      301 aaattagtaa taatttaatg agaaagctaa tcgctgaaag tcaattaaat acattaaaga
      361 aatta
//
LOCUS       DBNFCK010000258          365 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.262, whole genome shotgun sequence.
ACCESSION   DBNFCK010000258 DBNFCK010000000
VERSION     DBNFCK010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.262"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            128..295
                     /locus_tag="AC1PIW_002002"
     CDS             128..295
                     /locus_tag="AC1PIW_002002"
                     /inference="COORDINATES: protein motif:HMM:TIGR03731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gallidermin/nisin family lantibiotic"
                     /protein_id="HIP6606874.1"
                     /translation="MNNEDFNLDLVTISKENNSGASPRVTSKSLCTPGCKTGILMTCA
                     IKTATCGCHFG"
BASE COUNT          128 a           51 c           58 g          128 t
ORIGIN      
        1 gtataaatat ttcatgcatt ctaaataaaa aatttgttca ttctctataa ttatgaacaa
       61 attctgaact aggtatctta caatatattt gttcgaacaa agaaaaaata attggaggaa
      121 ttttattatg aacaatgaag attttaattt ggatcttgtc acaatctcaa aggaaaacaa
      181 ctcaggagct tcacctcggg taactagtaa atcattatgt actcctggat gtaagacggg
      241 tattttgatg acctgtgcaa tcaaaactgc aacttgtggt tgccattttg gataaaattt
      301 atttgtttag taactatctt tttgcaaatc ttgactaaga aagaaaggga ctctatatct
      361 tttcg
//
LOCUS       DBNFCK010000259          364 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.263, whole genome shotgun sequence.
ACCESSION   DBNFCK010000259 DBNFCK010000000
VERSION     DBNFCK010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.263"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            36..>364
                     /locus_tag="AC1PIW_002003"
     CDS             36..>364
                     /locus_tag="AC1PIW_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SSU0592/SSU0593 family protein"
                     /protein_id="HIP6606875.1"
                     /translation="MKHIVTKRNYKVGACAMALALLMTTAGGAVVNAEQHLESSVGLV
                     QRGDVLLKLKESNQSFNEGEVGNLEPVNPTVPPMASSVQKSWFSSFWGGVKTFWNALC
                     CSVGLTGL"
BASE COUNT          108 a           60 c           87 g          109 t
ORIGIN      
        1 ggaagcaaat atctatacaa aggagatgtt tcatcatgaa acatattgta acaaaacgta
       61 attataaagt tggcgcatgc gctatggcac ttgcattgtt gatgactact gcaggtggtg
      121 cagttgtaaa tgcagagcag caccttgagt catcagttgg cttagtacag cgtggtgatg
      181 tacttctaaa attgaaagaa tcaaaccagt ctttcaatga aggagaggtt ggaaatttag
      241 agcctgtcaa cccaactgtg cctccaatgg cttcttctgt acaaaaatca tggtttagtt
      301 cattttgggg tggtgtaaaa acattctgga atgcattatg ttgttctgtg ggactaactg
      361 ggct
//
LOCUS       DBNFCK010000260          364 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.264, whole genome shotgun sequence.
ACCESSION   DBNFCK010000260 DBNFCK010000000
VERSION     DBNFCK010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.264"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..211)
                     /locus_tag="AC1PIW_002004"
     CDS             complement(<1..211)
                     /locus_tag="AC1PIW_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HIP6606876.1"
                     /translation="MRVVFGIDVSKASSEVAILVNGEKVHGYTISNDAIGFARLLGDL
                     RTVHKPEIIFEATGVYSRRLQTFLDD"
BASE COUNT           96 a           86 c           70 g          112 t
ORIGIN      
        1 tatcatctag aaaagtttgg agacggcgag aatagacacc tgttgcttca aagatgattt
       61 ctggcttatg gacggttctc aaatcgccaa gtagccgagc aaagccgatg gcgtcattgg
      121 atatggtata gccatgaact ttctcaccat tgactagaat ggccacttct gaacttgcct
      181 tactcacatc aatcccaaaa actactcgca tgatattacc tctttgtctt gaatgattcc
      241 ttgttttagc gatgtcattt tcaatactcg acgtctggcg tcccacatac tttgataaca
      301 ttctttctaa aacaggtgtc ttgccagttt ttgttgcgac gtctagcgtc aaaagcaccc
      361 tcga
//
LOCUS       DBNFCK010000261          361 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.265, whole genome shotgun sequence.
ACCESSION   DBNFCK010000261 DBNFCK010000000
VERSION     DBNFCK010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.265"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..>361)
                     /locus_tag="AC1PIW_002005"
     CDS             complement(<1..>361)
                     /locus_tag="AC1PIW_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HIP6606877.1"
                     /translation="RGGVVHFVKVMINSLFINNRNDHLNGILTVGIFGYFGWFVTQLP
                     LWLIFIDIFIFTLLLFNDGNLKISKTEGLASLFVLPVQVAIIVAGMYFAWTPTAIGPN
                     AMISQGAQGRYFTPFLVY"
BASE COUNT          137 a           73 c           53 g           98 t
ORIGIN      
        1 gatagactaa aaacggagta aagtatcttc cttgtgcccc ttgagaaatc attgcatttg
       61 gtccaatagc tgtcggtgtc caggcaaaat acatgcccgc tacaatgata gctacctgaa
      121 cagggagtac aaataacgac gcaagacctt ctgttttact tatctttaaa tttccatcat
      181 taaataataa taacgtaaaa atgaaaatat ctatgaaaat taaccagaga ggtagttgag
      241 tgacaaacca gccaaaataa ccaaaaatac caaccgttaa aataccatta agatgatcat
      301 ttctattatt gataaataaa ctgtttatca tcactttcac aaagtgcacg acaccacctc
      361 t
//
LOCUS       DBNFCK010000262          328 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.266, whole genome shotgun sequence.
ACCESSION   DBNFCK010000262 DBNFCK010000000
VERSION     DBNFCK010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.266"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..>328)
                     /locus_tag="AC1PIW_002006"
     CDS             complement(<1..>328)
                     /locus_tag="AC1PIW_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HIP6606878.1"
                     /translation="HISKAFKTISLSVIYLLVPIFSLFIIENSYSQFSTLSSNSLWNN
                     LIIIGVLYLILVFTTNLRFASLLILSASVFIGISNQLLIDSRGAPLLFYNLFQITDGL
                     NVASSVA"
BASE COUNT          127 a           39 c           71 g           91 t
ORIGIN      
        1 gctacgctag aagctacatt taatccgtct gtgatttgaa aaagattata aaataataga
       61 ggagcaccac ggctgtcaat caaaagttgg ttacttatac caataaatac tgaagcggat
      121 agaattaata ggctggcaaa tcgtaagtta gttgtaaaga ccagtattag gtagaggact
      181 ccgataatga ttagattgtt ccaaagacta ttggaggaaa gagtagagaa ttgtgagtag
      241 ctattttcaa taataaataa tgaaaaaatg ggaactagaa gataaattac tgatagacta
      301 atcgttttga aggctttaga gatatgac
//
LOCUS       DBNFCK010000263          323 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.267, whole genome shotgun sequence.
ACCESSION   DBNFCK010000263 DBNFCK010000000
VERSION     DBNFCK010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.267"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..>323
                     /locus_tag="AC1PIW_002007"
     CDS             <1..>323
                     /locus_tag="AC1PIW_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HIP6606879.1"
                     /translation="STPFGLFSETSIGSFSSTEKLNLNGKTLKKVLLDSEWLIRLVFK
                     IEKEYSRELAYKINPANYQFGDRVVQLFSINDTKIEEVNIKFTKVYQLIDELCCDKYV
                     YFNCI"
BASE COUNT          118 a           39 c           51 g          115 t
ORIGIN      
        1 gctcaacacc atttggttta tttagtgaaa cttcaatagg ttcattttct tctacagaga
       61 aattaaactt gaatggaaaa acattaaaaa aagtactctt ggattcagag tggttaatac
      121 gtctagtttt taagattgag aaggaatatt ctcgagaatt agcctataaa ataaatccag
      181 ccaactatca atttggagat agagttgttc aattgttttc tataaatgat actaaaattg
      241 aagaagtcaa cataaagttc actaaagttt atcaacttat tgatgagctt tgttgtgata
      301 aatatgtata ttttaactgt att
//
LOCUS       DBNFCK010000264          320 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.268, whole genome shotgun sequence.
ACCESSION   DBNFCK010000264 DBNFCK010000000
VERSION     DBNFCK010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.268"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..269
                     /locus_tag="AC1PIW_002008"
     CDS             <1..269
                     /locus_tag="AC1PIW_002008"
                     /inference="COORDINATES: protein motif:HMM:NF016419.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HIP6606880.1"
                     /translation="KRSIDMKQIKNRLKKHMFWIGLISLVLMLVDNVLFCYFNIECSR
                     TLNQIEQTICIILSILVFLGVLNNKPDVKVEDIMKNGMSSKNQK"
BASE COUNT          113 a           39 c           59 g          109 t
ORIGIN      
        1 caaaaaggag tatagatatg aaacaaataa agaatcgttt aaaaaaacat atgttttgga
       61 ttggactcat ttcgttggta ttgatgctcg ttgataatgt cctattttgt tattttaata
      121 ttgaatgtag tcgtacctta aaccaaatcg agcagacgat ttgtatcatc cttagcattc
      181 ttgtattttt aggagtgcta aataataaac cggatgtgaa ggtagaagac atcatgaaaa
      241 acggtatgtc atctaaaaat caaaaataat gtttgtttag aaactactga tgaggtatct
      301 agttgtactt acagttcagt
//
LOCUS       DBNFCK010000265          319 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.denovo.269, whole genome shotgun sequence.
ACCESSION   DBNFCK010000265 DBNFCK010000000
VERSION     DBNFCK010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.denovo.269"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            complement(<1..>319)
                     /locus_tag="AC1PIW_002009"
     CDS             complement(<1..>319)
                     /locus_tag="AC1PIW_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HIP6606881.1"
                     /translation="QDKDKINLGFELFFYVNKVDGKSVLSLTNTGCSKNLGASSGRFS
                     ILSDKLEHYHQTITQIVENNNHVSGYNSCEITFLPENLRHANVMRTTNVREKVLSLFT
                     NMD"
BASE COUNT          101 a           52 c           46 g          120 t
ORIGIN      
        1 ttatccatat tcgtaaaaag tgataatact ttttctctga catttgttgt gcgcataaca
       61 ttagcatgac gaagattctc tggtaaaaat gttatttcac aagagttata ccctgatacg
      121 tgattattgt tttcaacaat ttgagtgata gtttgatgat aatgttccaa tttatctgat
      181 agaatagaaa acctgcctga gctagctcct aaattcttag aacatcctgt attagttaag
      241 cttagtacgc ttttaccatc tactttatta acataaaaga acaactcaaa tcctagattg
      301 attttatctt tgtcttgat
//
LOCUS       DBNFCK010000266         2552 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.guided.163, whole genome shotgun sequence.
ACCESSION   DBNFCK010000266 DBNFCK010000000
VERSION     DBNFCK010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.guided.163"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..250
                     /locus_tag="AC1PIW_002010"
     CDS             <1..250
                     /locus_tag="AC1PIW_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HIP6606882.1"
                     /translation="DYLKHHCKVTYTNLLTSGRLNAYLADIDRQAQERFERLIEGMKQ
                     AQGITEQLKAENALEWTGCLNNIRACAREIVEKEIIFA"
     gene            617..2536
                     /gene="tet(O)"
                     /locus_tag="AC1PIW_002011"
     CDS             617..2536
                     /gene="tet(O)"
                     /locus_tag="AC1PIW_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(O)"
                     /protein_id="HIP6606883.1"
                     /translation="MKIINLGILAHVDAGKTTLTESLLYTSGAIAEPGSVDKGTTRTD
                     TMNLERQRGITIQTAVTSFQWEDVKVNIIDTPGHMDFLAEVYRSLSVLDGAVLLVSAK
                     DGIQAQTRILFHALQTMKIPTIFFINKIDQEGIDLPMVYQEMKAKLSSEIIVKQKVGQ
                     HPHINVTDNDDMEQWDAVIMGNDELLEKYMSGKPFKMSELEQEENRRFQNGTLFPVYH
                     GSAKNNLGIRQLIEVIASKFYSSTPEGQSELCGQVFKIEYSEKRRRFVYVRIYSGTLH
                     LRDVIKISEKEKIKITEMCVPTNGELYSSDTACSGDIVILPNDVLQLNSILGNEMLLP
                     QRKFIENPLPMLQTTIAVKKSEQREILLGALTEISDGDPLLKYYVDTTTHEIILSFLG
                     NVQMEVICAILEEKYHVEAEIKEPTVIYMERPLRKAEYTIHIEVPPNPFWASVGLSIE
                     PLPIGSGVQYESRVSLGYLNQSFQNAVMEGVLYGCEQGLYGWKVTDCKICFEYGLYYS
                     PVSTPADFRLLSPIVLEQALKKAGTELLEPYLHFEIYAPQEYLSRAYHDAPRYCADIV
                     STQVKNDEVILKGEIPARCIQEYRNDLTYFTNGQGVCLTELKGYQPAIGKFICQPRRP
                     NSRIDKVRHMFHKLA"
BASE COUNT          825 a          435 c          591 g          701 t
ORIGIN      
        1 ggattatctg aagcatcact gcaaagttac atacaccaat cttcttacaa gcggcagact
       61 taacgcttac cttgccgaca ttgacagaca ggcacaggaa cgctttgaaa ggctcataga
      121 gggtatgaaa caggcacagg gcataacgga acagctaaag gcagaaaacg ccttagaatg
      181 gacaggatgc ctcaataaca taagggcttg tgcgagggag attgtggaaa aggaaattat
      241 ttttgcataa acagatgatt agtggcaggg ggaaatcctg ccgctttttc tgctttagtt
      301 tgtcagcttg acaaataaag ggttaaggaa tataattaga ttcagtatta tacaaggagt
      361 taataaatat gcggcaaggt attcttaaat aaactgtcaa tttgatagtg ggaacaaaaa
      421 gtagcagtcc cgtttcactt ttaatatggg gcttagtttt ttgtacccag tttaagaata
      481 cttttatcat gtaattttat atgcccgaaa acatataagt gttttggggc tattggagtt
      541 atttacccag tgataggagt atttatcact gggtattttt atgccctttt ttgggtgttg
      601 ataggaggaa aatcacatga aaataattaa cttaggcatt ctggctcacg ttgacgcagg
      661 aaagacaaca ttaacggaga gtttattgta taccagtggt gcaattgcag aaccagggag
      721 cgtagataaa ggcacaacaa ggacagatac aatgaatttg gagcgtcaaa ggggaatcac
      781 tatccagaca gcagtgacat cttttcagtg ggaggatgta aaagtcaaca ttatagatac
      841 gccaggccat atggattttt tggcggaagt ataccgttct ttatccgtat tagacggagc
      901 agtattatta gtttctgcaa aggatggcat acaggcacag acccgtatac tgtttcatgc
      961 actacagaca atgaagattc cgacaatttt tttcatcaat aaaattgacc aagaggggat
     1021 tgatttgcca atggtatatc aagaaatgaa agcaaagctt tcttcggaaa ttatagtgaa
     1081 gcaaaaggtt gggcagcatc cccatataaa tgtaacggac aatgacgata tggaacagtg
     1141 ggatgcggta attatgggaa acgatgaact attagagaaa tatatgtcag ggaaaccgtt
     1201 taaaatgtca gaactggaac aggaagaaaa caggagattc caaaacggaa cgttatttcc
     1261 cgtttatcac ggaagtgcta aaaacaatct ggggattcgg cagcttatag aagtgattgc
     1321 cagtaagttt tattcatcaa cgcctgaagg tcaatctgaa ctatgcgggc aggtttttaa
     1381 gattgaatat tcagagaaaa ggcggcgttt tgtttatgtg cgtatatata gcggaacatt
     1441 gcatttgagg gatgttatta aaatatctga aaaagagaaa ataaaaatca cagagatgtg
     1501 tgttccgaca aacggtgaat tatattcatc cgatacagcc tgctctggtg atattgtaat
     1561 tttaccaaat gatgttttgc agctaaacag tattttgggg aacgaaatgc tgttgccgca
     1621 gagaaaattt attgaaaatc ctctccctat gctccaaaca acgattgcag taaagaaatc
     1681 tgaacagcgg gaaatattgc ttggggcact tacagaaatt tcagatggcg accctctttt
     1741 aaaatattat gtggatacta caacgcatga gattatactt tcttttttgg ggaatgtgca
     1801 gatggaagtc atttgtgcca tccttgagga aaaataccat gtggaggcag aaataaaaga
     1861 gcctactgtt atatatatgg aaagaccgct tagaaaagca gaatatacca tccacataga
     1921 agtcccgcca aatcctttct gggcttctgt cgggttgtcc atagagccgc tccctattgg
     1981 aagcggagtg cagtatgaaa gcagagtttc acttggatat ttaaaccaat cgttccaaaa
     2041 tgcggttatg gagggggttc tttatggctg cgagcagggg ctgtatggat ggaaagtgac
     2101 agactgtaaa atctgttttg aatatggatt gtattatagt cctgtaagta cccccgcaga
     2161 ctttcggctg ctttccccta tcgtattgga gcaggcttta aaaaaagcag ggacagaact
     2221 attagagcca tatctccact ttgaaattta tgcaccgcag gaatatctct cacgggcgta
     2281 tcatgatgcc ccaaggtatt gtgcagatat tgtaagtact caggtaaaga atgacgaggt
     2341 cattctgaaa ggagaaatcc ctgccagatg tattcaagaa tacaggaacg atttaactta
     2401 tttcacaaat gggcagggag tctgcttgac agagttaaaa ggataccagc cagctattgg
     2461 taaatttatt tgccaacccc gccgcccgaa tagccgtata gataaggttc ggcatatgtt
     2521 ccacaagtta gcttaacagc ttgcaaaagt ca
//
LOCUS       DBNFCK010000267         1967 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.guided.184, whole genome shotgun sequence.
ACCESSION   DBNFCK010000267 DBNFCK010000000
VERSION     DBNFCK010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.guided.184"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            <1..1566
                     /locus_tag="AC1PIW_002012"
     CDS             <1..1566
                     /locus_tag="AC1PIW_002012"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIP6606884.1"
                     /translation="AVFLNQSVQYMGMIILGAIGFYLSMRLLMHCANRIAENLRNQAY
                     DVMQRLPISYFDDKPAGKIATRIVNDTETLRTQFYGTLVNAFNNIVRLLFTYGVLFYM
                     NRSLGWLMLLLIPLYIGIQFAYKKMTDKPMKDFYDARSDVNTQVNETMNGASLIQLFG
                     QEERIMKEFEVTADKMRRADNKIIWAQSLATWNLSGFLQNLVIAAILTVVGYQFLAGQ
                     DGVTAGKLFVYVNYIEGVFIALGALVQQFPNMLRSFETGKRLMALLEEELEDDCDRIL
                     EVEQGQVVFEHVNFSYEENRPVLKDITIRAEKGETVALVGHTGSGKSSIMNLLYRFYD
                     PQEGCVLIDGKNIRDYSRESLRSHMGIVLQDPYLFTGTIASNVSMNEEEADRTRIMQA
                     LEKVGAGPMLSRLEKGIDEPVVEKGAAFSSGERQLIAFARTLYSDPKILILDEATSHI
                     DTETEEIIQHAMEVVKEGRTTFIIAHRLSTIQNADQILVLDQGRIIEHGKHEELVARG
                     GVYAQMHEIQAKV"
     gene            1620..>1967
                     /locus_tag="AC1PIW_002013"
     CDS             1620..>1967
                     /locus_tag="AC1PIW_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIP6606885.1"
                     /translation="MKALIFDVDDTLYDQIQPFERALERHIEVAREQIEPLYLSFRRY
                     ADEVFEATAIGKMSLKDSHIYRMKHALADFGYQVSDATALAIQIDYDYFQGQIELSPV
                     FPEIFSWCQVQGIA"
BASE COUNT          569 a          339 c          469 g          590 t
ORIGIN      
        1 gctgttttct taaaccagtc agttcaatac atgggaatga ttattctggg agctattggc
       61 ttctacttat ctatgcgttt actcatgcat tgcgccaatc gtattgctga aaatctacgt
      121 aatcaagcct acgatgttat gcaacggttg cctatttctt attttgatga taagcccgct
      181 ggaaaaattg caactagaat tgtcaatgat acagaaactc tgcggacaca attttatggg
      241 accttagtta atgcctttaa caatattgtt cgtttgctct ttacatacgg tgtcctattt
      301 tatatgaata ggagtcttgg ttggctgatg ttactcttga taccactcta tatcggtatt
      361 caatttgctt acaagaaaat gacagacaag ccgatgaagg atttttatga tgcaaggagt
      421 gacgtcaata cgcaggtgaa tgagaccatg aatggtgcaa gtttgattca actttttgga
      481 caagaagagc gcatcatgaa agaatttgaa gtgactgctg acaagatgcg tcgggcagat
      541 aataagatta tctgggccca gtctcttgcc acgtggaatc tgagtggttt tctgcaaaat
      601 ttggtcattg ctgcgatttt aacggtagtc ggctatcaat ttcttgcggg acaggatgga
      661 gttacagctg ggaaactctt tgtttatgtc aactatatcg aaggagtctt cattgctttg
      721 ggagcgctgg ttcaacagtt cccaaatatg cttcgctcgt ttgaaacagg taagcgtcta
      781 atggcccttc tagaagaaga gctggaggac gactgtgatc gtattttgga agttgaacaa
      841 gggcaagtcg tgtttgaaca cgtgaacttt agctatgaag aaaacagacc agttttgaaa
      901 gatattacca ttcgagcaga aaaaggagaa acagttgctc tggttgggca tactgggtct
      961 ggtaagtctt ctattatgaa tctcctctat cgtttctatg atccacagga gggatgtgta
     1021 ctgattgatg ggaaaaacat ccgtgattat tctcgtgaaa gcctgcgcag tcacatgggg
     1081 attgttttgc aagatcccta tctctttaca gggacgattg ccagcaatgt ttccatgaat
     1141 gaagaggaag cagatagaac aagaatcatg caggcgctag aaaaagttgg tgcagggcct
     1201 atgctatctc gcttagagaa agggattgat gaaccggttg ttgagaaggg ggcggccttt
     1261 tcaagtgggg aacgtcaatt gattgccttt gcgcggacac tctactctga tcctaaaatt
     1321 ctgatattgg atgaagcaac ttctcatatt gatacggaga cggaagaaat tatccaacat
     1381 gccatggaag tggtgaaaga aggtcggaca acctttatta ttgctcaccg actttctacc
     1441 attcaaaatg cagaccagat tcttgtctta gatcaaggtc gaattattga acatgggaag
     1501 catgaagaat tggttgcacg tggtggagta tatgctcaaa tgcatgagat tcaagcaaag
     1561 gtgtaataag cagttaaaaa ttccaagctt ttgctggaat tttttgctat aattgactca
     1621 tgaaagcctt aatttttgat gtagatgata cgctttatga tcagattcaa ccgtttgaac
     1681 gtgctttgga aagacacatt gaagtagcaa gggagcaaat tgaaccactc tacctttcct
     1741 ttcgcagata tgcggatgaa gtatttgaag cgacagcaat tggaaagatg agtttgaaag
     1801 acagtcatat ttatcggatg aaacatgctt tggcagattt tgggtatcag gtgtctgatg
     1861 caacagcctt agctattcaa atagattatg attattttca agggcagata gagctcagtc
     1921 cagtgtttcc agaaatattt tcgtggtgtc aggtgcaagg aattgca
//
LOCUS       DBNFCK010000268         1338 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.guided.204, whole genome shotgun sequence.
ACCESSION   DBNFCK010000268 DBNFCK010000000
VERSION     DBNFCK010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.guided.204"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            25..774
                     /locus_tag="AC1PIW_002014"
     CDS             25..774
                     /locus_tag="AC1PIW_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIP6606886.1"
                     /translation="MMGSYDSQAKSMAKTAKHLEKRIERLDKVEQPRKETWVKMEAKG
                     ALDTGLHSLFRLQAGQVLMDEVYLFDVPQIGMTFGEKLALVGANGSGKTSFVRKLFRK
                     ELAGYYNPKLKIAYFAQDLINLDEEKTAFENVSCTSLQDRMMILNLLGMLGLSCDKAQ
                     QKVANLSGGERVRLSLAKVLLADANLLILDEPTNFLDMAAIEVLETFLKEYEGSVPLI
                     SHDQQFVETSVHSQWEIVQACLVKKSQENIF"
     gene            851..>1338
                     /locus_tag="AC1PIW_002015"
     CDS             851..>1338
                     /locus_tag="AC1PIW_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HIP6606887.1"
                     /translation="MKKRKNKPLEHDGNFISLRLNILFSIVIFLFLVLILRLADMQII
                     NHDFYSNKLSTASQKIISNGSIRGQIYDAQGKPLVENEIQQVVSFTRPNKMSAQEMKE
                     VANKLLQWVNVSDVTITQRDKADYYLADEEVYRQVVEALPKDKKYDSDGNYLSESTIY
                     SNA"
BASE COUNT          406 a          212 c          313 g          407 t
ORIGIN      
        1 tcgtctgagt ggaaagtcag tgccatgatg gggtcctatg atagtcaagc caagtctatg
       61 gccaagactg ccaagcattt ggaaaaacgg atagagcgtt tggacaaggt tgaacaacct
      121 agaaaggaaa cgtgggtaaa gatggaagcg aaaggtgcct tggatacggg tcttcacagt
      181 ctctttcgct tgcaggctgg tcaagttttg atggatgaag tgtatctatt tgatgtccct
      241 cagataggga tgacgtttgg agagaagtta gccttagtgg gggcgaatgg ttctggaaag
      301 actagttttg tccgaaaatt atttaggaaa gaattagcag gctattataa tcctaagtta
      361 aaaattgctt attttgcaca ggatttgata aaccttgatg aagaaaagac tgcttttgaa
      421 aatgttagtt gtacctctct tcaagatagg atgatgattc tcaatttatt aggtatgtta
      481 ggactttctt gcgataaggc tcagcagaag gtagcaaatc tgtctggtgg tgagcgtgtt
      541 cgtctctcct tagctaaggt attgttggcg gatgcaaatc ttttgatact agatgaaccg
      601 acaaattttc tagatatggc agctattgag gttttggaaa cattcttgaa agaatatgaa
      661 ggaagtgtcc ccttgatttc ccatgatcaa cagtttgtgg aaacatctgt tcacagtcaa
      721 tgggagatag tgcaagcttg tctcgtgaag aaatcacaag aaaatatttt ctgaaaagga
      781 ggctctgtct aaaaaatatt ttttgcctgt ggtataataa gaaggatatt tctagaatga
      841 ggtctttgaa gtgaaaaaaa gaaagaacaa gcctttggaa catgatggaa attttatttc
      901 ccttcgttta aatattttat tttctatcgt gattttcctt ttcctagttt tgatattgcg
      961 tctggcagat atgcagatca tcaatcatga tttttatagt aataagctgt ctactgccag
     1021 tcagaaaatc atcagcaatg gctctattcg cgggcagatt tacgatgcgc agggaaaacc
     1081 tttggttgaa aacgagattc agcaggtcgt ctccttcaca cgtcccaaca agatgtctgc
     1141 tcaggaaatg aaagaagtag ctaataaact cttgcagtgg gtcaatgttt cagacgtaac
     1201 aattactcaa cgtgataagg ctgattatta tttggcagat gaagaggttt atcgtcaggt
     1261 agtcgaggcc ttgccaaaag ataaaaaata cgattccgat ggtaactact tgagcgaatc
     1321 aacaatttat tctaatgc
//
LOCUS       DBNFCK010000269         1309 bp    DNA     linear   BCT 09-APR-2026
DEFINITION  TPA_asm: Streptococcus suis strain DA1_18
            SAMN40446024-rid29426533.guided.205, whole genome shotgun sequence.
ACCESSION   DBNFCK010000269 DBNFCK010000000
VERSION     DBNFCK010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40446024
            Sequence Read Archive: SRR28339520
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus suis
  ORGANISM  Streptococcus suis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-APR-2026) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using the SAUTE guided assembler with
            antimicrobial resistance genes and Stx operons as baits to increase
            sensitivity and specificity for those genes. Note, contigs derived
            from the SAUTE guided assembly may overlap de-novo contigs from
            SKESA, and/or other SAUTE contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names, which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 09-APR-2026
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT003171460.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT003171460.1
            Genome Coverage       :: 36x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/09/2026 16:52:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2025-05-06.build7983
            Genes (total)                     :: 2,017
            CDSs (total)                      :: 1,969
            Genes (coding)                    :: 1,909
            CDSs (with protein)               :: 1,909
            Genes (RNA)                       :: 48
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 28 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 9 of 60
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Streptococcus suis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40446024-rid29426533.guided.205"
                     /strain="DA1_18"
                     /host="swine"
                     /db_xref="taxon:1307"
                     /geo_loc_name="USA"
                     /collection_date="2018"
     gene            27..764
                     /gene="erm(B)"
                     /locus_tag="AC1PIW_002016"
     CDS             27..764
                     /gene="erm(B)"
                     /locus_tag="AC1PIW_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HIP6606888.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
     gene            934..1194
                     /locus_tag="AC1PIW_002017"
                     /pseudo
     CDS             934..1194
                     /locus_tag="AC1PIW_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
BASE COUNT          499 a          217 c          216 g          377 t
ORIGIN      
        1 gatgtaatca cttcaggaga gattacatga acaaaaatat aaaatattct caaaactttt
       61 taacgagtga aaaagtactc aaccaaataa taaaacaatt gaatttaaaa gaaaccgata
      121 ccgtttacga aattggaaca ggtaaagggc atttaacgac gaaactggct aaaataagta
      181 aacaggtaac gtctattgaa ttagacagtc atctattcaa cttatcgtca gaaaaattaa
      241 aactgaatac tcgtgtcact ttaattcacc aagatattct acagtttcaa ttccctaaca
      301 aacagaggta taaaattgtt gggaatattc cttaccattt aagcacacaa attattaaaa
      361 aagtggtttt tgaaagccgt gcgtctgaca tctatctgat tgttgaagaa ggattctaca
      421 agcgtacctt ggatattcac cgaacactag ggttgctctt gcacactcaa gtctcgattc
      481 agcaattgct taagctgcca gcggaatgct ttcatcctaa accaaaagta aacagtgtct
      541 taataaaact tacccgccat accacagatg ttccagataa atattggaag ctatatacgt
      601 actttgtttc aaaatgggtc aatcgagaat atcgtcaact gtttactaaa aatcagtttc
      661 atcaagcaat gaaacacgcc aaagtaaaca atttaagtac cattacttat gagcaagtat
      721 tgtctatttt taatagttat ctattattta acgggaggaa ataattctat gagtcgcttt
      781 tttaaatttg gaaagttaca cgttactaaa gggaatggag ataaattatt agatatacta
      841 ctgacagctt ccaagaagct aaagaggtcc ctagcgccta cggggaattt gtatcgataa
      901 ggggtacaaa ttcccactaa gcgctcggga ccccttgtag gaaaatgtcc taagtgtggc
      961 aacaatattg tattaaaaaa atcgttttat ggttgttcaa attatcctga atgtaagttt
     1021 actttagctg aacattttag aaagaaaaaa ctaaccaaaa cgaatgtaaa agaattactg
     1081 gagggaaaag aaaccctggt aaaaggaatc aaaaacaaag agaaaaagcc ctacaatgcc
     1141 gttgtaaaaa ttggggaaaa gggatatatt gattttatct ctttttcaaa ataaacataa
     1201 aagcccttta aagagggctt ttatatatta atcacaaatc acttatcaca aatcacaagt
     1261 gattaatcac aaatcacaag tgatttgtga ttcttaatga tacaatatt
//
